LOCUS       DATRSG010000001        75741 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRSG010000001 DATRSG010000000
VERSION     DATRSG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 75741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75741
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.001"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..226
                     /locus_tag="VBD89_000001"
     CDS             <1..226
                     /locus_tag="VBD89_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO7134566.1"
                     /translation="FAKYPVAIYNNLLRWIISFVIPFAFTAYYPAAYFLQDRNVYFNI
                     GGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            851..1156
                     /locus_tag="VBD89_000002"
     CDS             851..1156
                     /locus_tag="VBD89_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO7134567.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            1156..2004
                     /locus_tag="VBD89_000003"
     CDS             1156..2004
                     /locus_tag="VBD89_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO7134568.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            2001..2723
                     /gene="fabG"
                     /locus_tag="VBD89_000004"
     CDS             2001..2723
                     /gene="fabG"
                     /locus_tag="VBD89_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO7134569.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            2716..3021
                     /locus_tag="VBD89_000005"
     CDS             2716..3021
                     /locus_tag="VBD89_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7134570.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            3005..3481
                     /gene="fabZ"
                     /locus_tag="VBD89_000006"
     CDS             3005..3481
                     /gene="fabZ"
                     /locus_tag="VBD89_000006"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7134571.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            3471..4400
                     /locus_tag="VBD89_000007"
     CDS             3471..4400
                     /locus_tag="VBD89_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134572.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            4393..5271
                     /locus_tag="VBD89_000008"
     CDS             4393..5271
                     /locus_tag="VBD89_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134573.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            5268..7271
                     /locus_tag="VBD89_000009"
     CDS             5268..7271
                     /locus_tag="VBD89_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO7134574.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            7268..8221
                     /locus_tag="VBD89_000010"
     CDS             7268..8221
                     /locus_tag="VBD89_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO7134575.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            8218..10413
                     /locus_tag="VBD89_000011"
     CDS             8218..10413
                     /locus_tag="VBD89_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO7134576.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            10418..11629
                     /locus_tag="VBD89_000012"
     CDS             10418..11629
                     /locus_tag="VBD89_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO7134577.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            11598..12173
                     /locus_tag="VBD89_000013"
     CDS             11598..12173
                     /locus_tag="VBD89_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO7134578.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
     gene            12516..12857
                     /locus_tag="VBD89_000014"
     CDS             12516..12857
                     /locus_tag="VBD89_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134579.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            12891..13406
                     /locus_tag="VBD89_000015"
     CDS             12891..13406
                     /locus_tag="VBD89_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134580.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            13458..13598
                     /locus_tag="VBD89_000016"
     CDS             13458..13598
                     /locus_tag="VBD89_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134581.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKQL
                     HS"
     gene            13697..16114
                     /locus_tag="VBD89_000017"
     CDS             13697..16114
                     /locus_tag="VBD89_000017"
                     /inference="COORDINATES: protein motif:HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7134582.1"
                     /translation="MKRYDAIINGVALDIQAQEIEKLKTIADVRRVYVSQEYVQTKPL
                     LSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFKHQALKIKEPNRAKYNKTSIEKL
                     IHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMHGMHVTGIVGANDDNQKLYGVAP
                     NAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKADVVNMSLGTPAGFVHEGKDYPE
                     LEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLAENYDTALIANPALDDNTLAVAS
                     MENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASKTIIGLAVDLGAEAPSELSKHFD
                     LSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILLYNNAKVKDDLGSQLLVESEAAK
                     FNIARITRSTYNNIKNNSNKIITILTERQAIDNSLAGQLSSYSSWGPTPDLRLKPEIT
                     APGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLKQYITDKKIPVDNAADFIKLLLM
                     NTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVTTVVATVTGTNDNNADGKLELRE
                     LKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEKGFRTQNSEHLVSKKADAVTRKV
                     TVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKDTEGVADLHLPFLGFYGSWTEQK
                     AIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFTTNGMLSLPIYNNTVFFSPNSPF
                     YDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRSHDVRKLYRLDYRNSFAMMPDSI
                     WDGKIKD"
     gene            16160..19496
                     /locus_tag="VBD89_000018"
                     /pseudo
     CDS             16160..19496
                     /locus_tag="VBD89_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19680..20765
                     /locus_tag="VBD89_000019"
     CDS             19680..20765
                     /locus_tag="VBD89_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO7134583.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIELKEKMLTPN"
     gene            20786..21604
                     /locus_tag="VBD89_000020"
     CDS             20786..21604
                     /locus_tag="VBD89_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO7134584.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            21601..21711
                     /locus_tag="VBD89_000021"
     CDS             21601..21711
                     /locus_tag="VBD89_000021"
                     /inference="COORDINATES: protein motif:HMM:NF013590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134585.1"
                     /translation="MRANIPVRNFQEFYDAFGVKKGDSMYLKPEKRLTLW"
     gene            21743..22774
                     /locus_tag="VBD89_000022"
     CDS             21743..22774
                     /locus_tag="VBD89_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7134586.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            22809..23808
                     /locus_tag="VBD89_000023"
                     /pseudo
     CDS             22809..23808
                     /locus_tag="VBD89_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            23843..24904
                     /locus_tag="VBD89_000024"
     CDS             23843..24904
                     /locus_tag="VBD89_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7134587.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            25028..26257
                     /locus_tag="VBD89_000025"
     CDS             25028..26257
                     /locus_tag="VBD89_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134588.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            26267..26902
                     /locus_tag="VBD89_000026"
     CDS             26267..26902
                     /locus_tag="VBD89_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7134589.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGGRHITPKDQRVIIV
                     HQAFAKTNHLKVGDKLMFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYP"
     gene            26906..27061
                     /locus_tag="VBD89_000027"
     CDS             26906..27061
                     /locus_tag="VBD89_000027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134590.1"
                     /translation="MLENTAFSDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKS
                     FIKKQSY"
     gene            27162..27290
                     /locus_tag="VBD89_000028"
     CDS             27162..27290
                     /locus_tag="VBD89_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7134591.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            27299..27619
                     /locus_tag="VBD89_000029"
     CDS             27299..27619
                     /locus_tag="VBD89_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7134592.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            27716..28387
                     /locus_tag="VBD89_000030"
     CDS             27716..28387
                     /locus_tag="VBD89_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134593.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(28444..29889)
                     /locus_tag="VBD89_000031"
     CDS             complement(28444..29889)
                     /locus_tag="VBD89_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO7134594.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            30150..30935
                     /locus_tag="VBD89_000032"
     CDS             30150..30935
                     /locus_tag="VBD89_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7134595.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            complement(31013..31651)
                     /locus_tag="VBD89_000033"
     CDS             complement(31013..31651)
                     /locus_tag="VBD89_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO7134596.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVASISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            31868..32524
                     /locus_tag="VBD89_000034"
     CDS             31868..32524
                     /locus_tag="VBD89_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7134597.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            32521..33294
                     /gene="fetB"
                     /locus_tag="VBD89_000035"
     CDS             32521..33294
                     /gene="fetB"
                     /locus_tag="VBD89_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO7134598.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            33458..34276
                     /locus_tag="VBD89_000036"
     CDS             33458..34276
                     /locus_tag="VBD89_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO7134599.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(34313..35197)
                     /locus_tag="VBD89_000037"
     CDS             complement(34313..35197)
                     /locus_tag="VBD89_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7134600.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGVRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            35390..36448
                     /locus_tag="VBD89_000038"
     CDS             35390..36448
                     /locus_tag="VBD89_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7134601.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            36462..37454
                     /locus_tag="VBD89_000039"
     CDS             36462..37454
                     /locus_tag="VBD89_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO7134602.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            37662..39212
                     /locus_tag="VBD89_000040"
     CDS             37662..39212
                     /locus_tag="VBD89_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO7134603.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            39279..40304
                     /locus_tag="VBD89_000041"
     CDS             39279..40304
                     /locus_tag="VBD89_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO7134604.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            40321..42120
                     /gene="uidA"
                     /locus_tag="VBD89_000042"
     CDS             40321..42120
                     /gene="uidA"
                     /locus_tag="VBD89_000042"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO7134605.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            42149..42820
                     /locus_tag="VBD89_000043"
     CDS             42149..42820
                     /locus_tag="VBD89_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO7134606.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            42937..43554
                     /gene="eda"
                     /locus_tag="VBD89_000044"
     CDS             42937..43554
                     /gene="eda"
                     /locus_tag="VBD89_000044"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO7134607.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGPGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            43571..44971
                     /gene="uxaC"
                     /locus_tag="VBD89_000045"
     CDS             43571..44971
                     /gene="uxaC"
                     /locus_tag="VBD89_000045"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO7134608.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            44989..46035
                     /gene="uxuA"
                     /locus_tag="VBD89_000046"
     CDS             44989..46035
                     /gene="uxuA"
                     /locus_tag="VBD89_000046"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO7134609.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            46159..46998
                     /locus_tag="VBD89_000047"
     CDS             46159..46998
                     /locus_tag="VBD89_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7134610.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            47151..47963
                     /locus_tag="VBD89_000048"
     CDS             47151..47963
                     /locus_tag="VBD89_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7134611.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            47979..49769
                     /locus_tag="VBD89_000049"
     CDS             47979..49769
                     /locus_tag="VBD89_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO7134612.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(49827..50912)
                     /locus_tag="VBD89_000050"
     CDS             complement(49827..50912)
                     /locus_tag="VBD89_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7134613.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            51122..52126
                     /gene="ccpA"
                     /locus_tag="VBD89_000051"
     CDS             51122..52126
                     /gene="ccpA"
                     /locus_tag="VBD89_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO7134614.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            52258..53724
                     /locus_tag="VBD89_000052"
     CDS             52258..53724
                     /locus_tag="VBD89_000052"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO7134615.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            53769..54767
                     /locus_tag="VBD89_000053"
     CDS             53769..54767
                     /locus_tag="VBD89_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7134616.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            54769..56103
                     /locus_tag="VBD89_000054"
     CDS             54769..56103
                     /locus_tag="VBD89_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7134617.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            56560..58503
                     /gene="thrS"
                     /locus_tag="VBD89_000055"
     CDS             56560..58503
                     /gene="thrS"
                     /locus_tag="VBD89_000055"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO7134618.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            58724..59428
                     /locus_tag="VBD89_000056"
     CDS             58724..59428
                     /locus_tag="VBD89_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7134619.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            59526..60545
                     /locus_tag="VBD89_000057"
     CDS             59526..60545
                     /locus_tag="VBD89_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO7134620.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            60712..61278
                     /locus_tag="VBD89_000058"
     CDS             60712..61278
                     /locus_tag="VBD89_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7134621.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(61327..61977)
                     /locus_tag="VBD89_000059"
     CDS             complement(61327..61977)
                     /locus_tag="VBD89_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7134622.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(61989..62684)
                     /locus_tag="VBD89_000060"
     CDS             complement(61989..62684)
                     /locus_tag="VBD89_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7134623.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(62697..63497)
                     /locus_tag="VBD89_000061"
     CDS             complement(62697..63497)
                     /locus_tag="VBD89_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7134624.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(63509..64264)
                     /locus_tag="VBD89_000062"
     CDS             complement(63509..64264)
                     /locus_tag="VBD89_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7134625.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
     gene            64604..65314
                     /locus_tag="VBD89_000063"
     CDS             64604..65314
                     /locus_tag="VBD89_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7134626.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            65307..66656
                     /gene="vicK"
                     /locus_tag="VBD89_000064"
     CDS             65307..66656
                     /gene="vicK"
                     /locus_tag="VBD89_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO7134627.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            66660..67469
                     /locus_tag="VBD89_000065"
     CDS             66660..67469
                     /locus_tag="VBD89_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7134628.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            67472..67840
                     /locus_tag="VBD89_000066"
     CDS             67472..67840
                     /locus_tag="VBD89_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO7134629.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            68015..68701
                     /locus_tag="VBD89_000067"
     CDS             68015..68701
                     /locus_tag="VBD89_000067"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO7134630.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            68709..72248
                     /gene="smc"
                     /locus_tag="VBD89_000068"
     CDS             68709..72248
                     /gene="smc"
                     /locus_tag="VBD89_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO7134631.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            72339..73136
                     /locus_tag="VBD89_000069"
     CDS             72339..73136
                     /locus_tag="VBD89_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7134632.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            73136..73960
                     /locus_tag="VBD89_000070"
     CDS             73136..73960
                     /locus_tag="VBD89_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7134633.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            73960..75570
                     /gene="ftsY"
                     /locus_tag="VBD89_000071"
     CDS             73960..75570
                     /gene="ftsY"
                     /locus_tag="VBD89_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO7134634.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
BASE COUNT        25746 a        11496 c        14986 g        23513 t
ORIGIN      
        1 ctttgctaag tatcctgtcg caatatataa taatctattg cgttggatca ttagttttgt
       61 cattccattt gcttttacag cttattatcc cgctgcttac ttcttacaag atagaaatgt
      121 ctattttaat attggtgggg ttatcttgat ttccttgatt tcttttatgg tatcactaat
      181 actttggcat aaaggagtag aagtatatga gagtgcgggt tcttgattag taaggcaagc
      241 cattaatttt tttgagtgtt tagaagtttt aggcttacta acttagcttt gttagtgatg
      301 atatactccc cttatagtgt ctagtgaatt tttgtttttt tactgtccac tataagggga
      361 gtatatcagg tgaagaggga ctattagtgt tgagaataat gtccctcata gaacaataat
      421 taccatatct ttaccaaaat aaagttaaaa acgctcttag atgtgctttc taagagcgtt
      481 ttttggtcta taaaaggtaa gcaaaaagca gatgtcattt atgaaagtgt tgacaaagat
      541 gaaatacttt gttactattt taacataata atgatatttt aattagagtg tgaatttata
      601 attaattttt acaatgttta ttttaaattt aaactaatgt ttatagcagt gttttactca
      661 tgattttgtg aattcaacac ttaagtcatg acctcatttt aaagagtttc agacgacaca
      721 gaaaggaaga aaacataaga aaggcaatgc acggcttgtc catagtagtg tttgaagtag
      781 atgtttagaa atattttatt ttccaacagt actgatatca gagcgaagat gattagttga
      841 aggtgataat atgggacgtt ctaatatttt gagtaggtgt gaacaatgtt ctgataatca
      901 atgtggcttg tataaaacgc ggcttaaaga caagttacta ttagtagtcc aactggttgg
      961 aaatattagg tattctgagt ttcttacgga aggtggtgta tatcataagg gagaaacctt
     1021 gttggaagtt ataaccttag gaatttctta tgaatataaa agttatgggg atatcaaagt
     1081 aatcaagaag tactgtgctg atggcagtgt cgttgattat aacatgccaa tactattaat
     1141 ggaaaggatt gaataatgaa aacagtaata atatatggag gacagggtaa tgttcagtca
     1201 aaacaccttt gtcaactagt taaaactcca aaaggtgaaa ttcttttttc aaagttacaa
     1261 gcaactgatt tagaatcata tcagttaatg atggatgtca ttaaccacaa ggtaagttta
     1321 gacaatgttc atgcgtctat gttagcaaat tttttatata atgattggct aacaaacgat
     1381 gaaaaaatag tatctgggat aggattttca gctcatagcg ccggtatttt caatgtgtta
     1441 tatgctagtg gtagtactag ttttgaaaac attattcact ttattaaacg tcgtgcccaa
     1501 cttatagaaa attgtcatgt gtctgaagag ctttggcttt tgatggttga aaacattgat
     1561 atgtttagta aggatgtttt aaaagaacag tctgagaaag taaaattagc tattattact
     1621 gatgatagag ctggtgtaat tgctatggat caagtcaacc gttctttctt tgatgacgtg
     1681 atgactaaca aaggttattt ttataaatta aagcagttag gcgtaaaagc accatatcat
     1741 actgattttt taaatgaatc taaagagcag tataaacaat tgattgaaag cctagatatt
     1801 tcgcaaaatg atgactatga ctatattttt cattgtgatc aattggtaga tgaaatttta
     1861 tatcagtgga ccaatctctt taactggcgt gcgattaagg aaactatcat agaaaaggat
     1921 gcggagatta ttgatatttc tcctaatagg tttatttcta gtcagttgca aaaaatgaaa
     1981 aagaggggga ggaaacagtt atgaaagtag caatagttac tggtggtacc agaggaattg
     2041 gaagggctat tacaaaagag ttatacaaag aaggttacaa agtcattgct atttataata
     2101 gcaatgatgc taaggcaaga gctttacaag aggagttacc aaaattagac gtttataaat
     2161 gcaatatttc agatgcaaaa gcagtacaaa aattggtgac taaaattttt agagaatatg
     2221 gcggtattga ttgtttagtt aataatgctg gtattgttag agatggcttt ttcttgatga
     2281 tgtctaagga aaaatggatg gatgtcatta acataaatat tatggggcta gttaacatga
     2341 gtaaagccgt gttaaaaatc atgaaggcta agcgtataca gggaaaggtt atcaatattt
     2401 cttcaactag tggtatagca gggcagatag ggcaagcgaa ctattcagca acgaaaggcg
     2461 ctatcatttc cattactaaa actctagcga aagaatttgc atcagatggt attacgatta
     2521 attgtgtaag tccaggattc attgaaacag atatgacaaa tgaattgcaa aataaagaag
     2581 agctaaaaga gcatcttatt cctctaaaaa ggtttggaca acctgaagag gtggcttggc
     2641 tcgtaagctt tttagcaagt gagaaggcaa actatattac aggaaaaaat attgtgattg
     2701 atggaggaat gattaatgat taactgggct gaagaacgta aaataataaa aacacgtcaa
     2761 gaagtagttg atgaaattaa aggtattttg attgactctt taatgttgga tattgataaa
     2821 gatctgatta tgaatgatca accattattt ggtcgtggat tagagctaga ctctattgat
     2881 gccctagaac tgtcaatagg gatttcaaca acgtttggtg ttgaactaaa tgatgatgat
     2941 atttcaatat tatcatcagt aaatagacta gcagattttg tgattgaaaa tagtgaggat
     3001 atagatgacc aagctaagta gtgaagaaat tataaaaatg ttacctcatc gctatccgtt
     3061 tctaatggtg gatagagtgc agagttataa aaaaggtatc attgaatgtc gtaagaatat
     3121 tactatcaac gaaccttact ttcaaggaca ttttccacaa cgtcctatag ttccgggtgt
     3181 tttgatgatt gagatggcag ctcaatctgc agctttactt tatattttag atgcacttga
     3241 tggtagcctc ccctctcttg atgaattaac agcagagaaa atagcagaaa aagtaggtta
     3301 tttagccagt gtgaagaatt tcaaattcaa gaaaattgtt actccaggag accagctaaa
     3361 cattacatgt aaatctcaat caaaattggg gcatctttta gaaattcaag tcgttattag
     3421 agatgagaat aaaaaagagg ttgcctcagg aaggatgttg gtatcagaaa atggaaatta
     3481 aactcaaaaa tattggaaaa aaatatggtt cttttgaagc attgagagac attaatctca
     3541 tctttgaaga gggaaaattt tatggccttc ttggacctaa tggagctggt aaaacaactt
     3601 tatttaacct tttaattcaa aattttaagc aaacgtcagg cgatattaag tgggaggtgg
     3661 atggaaaacc tctatctatt aaagattttt atcgtcacat tggtattgtc ttccaaagta
     3721 atcgcttaga tgataattta acggttgagg aaaatctaat ttctagaggt gctttgtacg
     3781 gtttatctaa atcacaagta cgtaatcgcc ttaaagatct tcaaacgtat ctagatatca
     3841 cagcaataaa aaaacaaaaa tatggcagtt tatcaggagg tcaaaaacga aaagttgata
     3901 ttgcacgcgc cttgctgcca caaccttctc tattattatt agatgaacca acgactggtt
     3961 tagaccctca atctcgtcgc gatttatggg atgctatcgc acaactaaat caacaatcgc
     4021 agatgacagt tgtactaatt actcattatc ttgaagaaat gtctgcttgt gatgttttaa
     4081 atgttcttat tgaaggaaac atttattatt cgggagatat taaaagcttt attgagcaac
     4141 attccacaac aaacttgaat gtggtattga aaccagaaaa gtcattagac caactttcga
     4201 tagctgattt tgttaataaa tgtcaagtcc tttcagaaag ggagatagta tttaaggata
     4261 tttctgttga ggaaatgatg caggttatct cagacaatca agataaatct attattgaga
     4321 catttaatgt tgaatattcg aacttagagg cggcgtatct caacttgctt agagaaaaag
     4381 aaaggaaaaa caatgtttga actaatttct agaaatcgta aagtttacac gcgagatcgg
     4441 ttagcgtttt tcatgtcttt tttatcagtt attatcttaa ttttggtcta tcagattttc
     4501 ttgggaaaaa ttcaattaga tgctataaaa gcagcattga ggagtaatac agtatccagt
     4561 gatactattc acatggttaa ttattggctc attgcaggtt tgacgactat tatttcaatg
     4621 actagtacat taggtgcctt tggagttatg gtgtccgaca aagaaaaaaa attaaatgaa
     4681 gattttaagg tcagtcctat atcaaatatc aaaattgaat tgtcctatgc tatatttgcc
     4741 gttttatttg gtattgtcat gacgatgttc tcttgcatct ttgctatcgg tatttttaac
     4801 ggctttaatt ccttgctaga gtattcagct atggattatt taactatttt agggatcgtt
     4861 tccttaggga ctgttttatc tgcggcgatg attttaccta ttttagcctt tattcatact
     4921 agttcagctt ttacgactct tagcacaatt gtagggactt ttattggttt tatttcagga
     4981 gtatatctct caattggctc ggtaggaaaa gcattacaac aggttatgac atggtttcca
     5041 ttgactcaaa tcaattctct tttgaaacag gtcttaatga agggttctat tgcgaaggta
     5101 tttgacaaag ccaacgaagc cactgtctct aactataaag aatcatatgg tgttgttttg
     5161 cgtaatgctg atggagaaag gctgagtaat cactttatgt tgatttatat cattgccctc
     5221 attcttattt tattggcaat tcattttatt attaaaaagg ttaaaaaatg aaagatgata
     5281 ataaattaaa gatttctgaa gcttccttag aagattattc tgaagtggtt catttattta
     5341 ataggaatca tgtttatcaa tttccagacg gtaggccttt aactgttgat gaccttgatt
     5401 taacgttaaa agttaaagaa gtgacacatt tatttttact gaaaaatcat ggtgttttga
     5461 ttggaacatc agctttcttt aaatttatta cctatggttg tttagattgg aatagtagct
     5521 ttagtggttt tcttttaatt gattcgaaaa gtcgtagtgg acaggcaatc acttatctat
     5581 ataaaactat cttaaagaag ataacaaaac tgaagttttc taatatctat accgaaatca
     5641 gtaactacaa taaaccctcc ttagctttat caaaattgaa tggcttcaaa gagtatgata
     5701 agacatatga agacatactg cattgtcgat cattgcgtag tcacctccct aagatattga
     5761 atacgtttcg tatttcgaat tattatggta aaacatacga catatcaact tttcaaatta
     5821 tggaggaaat tgaaaatccc ttggaagagg agacagaaat taggaccaaa gtctctgatg
     5881 aagaaatatt atttaaagca gaagatagtg cttcacttcc ttattattta aaaatgagct
     5941 tgtttcaaat ggaaattgct aagttagata accgttatgt tttacaagtt gactttttat
     6001 cggaacaagt aaagagggtt cgggttaaaa ctggaaaata tcacttggca aacttaacga
     6061 gggctcaccc tagcctaact ttaagtcgtt ttgccaatta ttattacatt caagcaactg
     6121 tggaaaccct ttatggtaat atcgatgttc aactggaacg tcgaaaaaag cattatagag
     6181 atgcaactat ttgtcttaag agaacttttc aaggttatga tttacttatc tctccaaatg
     6241 gcagccttat ttttgaaaag cagaaaagaa agatacttga agatagtttt ttgattttta
     6301 gccaaccttt ggacaagaaa ttagttgtaa aagaagaaga aaatcacatt acaatcaaat
     6361 gtttctatca aggtgcttta attgagaaaa tcatgacatt tacaagtgac gaagaaatca
     6421 cttgtgttta taaatgtaat caaaaggcta aagagatgtt cccaaaatta ttgaaacaga
     6481 ctttcaagtt acactgccaa gagcagttga ttagagatag tgaaggttat cttgtgaatg
     6541 ttccaggaag ttacccgatt gagcatgatg attttcttcg agcagataag tttgaagata
     6601 gacaattcca ttattacctc ccgaatgaag ataaaatgat ttcctattct cctcctggca
     6661 aagccagcaa tcaaatgcaa tttagaccct tatgtcttat tgatacagat agcctttctt
     6721 ttccactgac ttatcatttt aggatttcac aagcatcatc ggaggaggcg ttaatcaatc
     6781 taaaaaaaca gccaatatgg gacagcaact accaagcgtc agcaactgac cttttaaaac
     6841 atatctctaa cctaacatta gaagaagaga aggattatgg catcaagaga atgatagcta
     6901 acagaaagca ttatcctagt cacaaactgg ttttagccta taatcagatt gttttgccta
     6961 agaatgaaat tccaagagac agtgaattat attctatttc ttttgattat cgaattagag
     7021 gaaagtttgt ccaaatccgc caaggggaac atgtcaaata tgataataaa tcctatgttt
     7081 tggaaaatag tcaacaatta gtattgtatg tagctagcga tgataaatat atactaattt
     7141 ctgctaaaaa tggaatattt tattcttaca aagagaacaa tcatttaaaa ataaggtgca
     7201 tgtttaaaag gaactcacct tatgctacaa atgtgagcat tacagaatat agaaagtgtg
     7261 aagaaaaatg aatactattt ataatacatt gagaacagat aaaggttata aagtttatga
     7321 ggggtattta tatgaaatta ctggtgaaga atgtgaagaa gccttagacc ttgtgattcc
     7381 taagaatatt gtatttgcag atacagatac ttgtggctac acttttttac tcaatgaaga
     7441 tggaacagtt tatgatgatg tgactttcta caaatttgat gataaatatt ggttggctag
     7501 tcataaagct ttggattctt atttagacaa catcaatttt gactataccg taacagatat
     7561 ttctgacgag tataaaatgc tgcaaattga aggaagatat tcgggagaaa ttgctcagtc
     7621 attttatgaa tatgatattt caacacttaa ttttcgtact ttgatagaga tgacttataa
     7681 aggtgagaaa ggttatcttg ctagatttgg tttttctgga gaatttggct atcaattttt
     7741 cctaccatct tctatttttg ctacttttgt ttcggatgtc tgtgaaggta tagcagagta
     7801 tggggatgaa cttgatagat atttaaggtt tgaagtggga caacccatta ctgatattta
     7861 tcaacaagaa gaatattctt tatatgaaat aggttattct tggaatctag atttcacaaa
     7921 ggaagaattt agaggtcgcg atagcttgtt agagcacatc agatcagcaa cagttaaaag
     7981 tgttggattc tcaacgaagg aaaaactcgc ttcaggaaca ccagtgctat ttgatgacca
     8041 aattgttgga aagatttttt ggatagcaga cgagaaagac tcttcggaaa attacctagg
     8101 tttgatgatt gttaaccaaa catatgctca ttcaggagtt acttttgtaa cagaagatgg
     8161 ccaaattttg aaaacacaat caagccctta ttgtatccca gaaagttgga acaaagaatg
     8221 agcgtatatg ttagtggaat aggaattatt tcttctttgg gaaagaatta tagcgagcat
     8281 aaacagcatc tcttcgactt aaaagaagga atttctaaac atttatataa aaatcacgac
     8341 tctattttag aatcttatac aggaagcata actagtgacc cagaggttcc tgagcaatac
     8401 aaagatgaga cacgtaattt taaatttgct tttaccgctt ttgaagaggc tcttgcttct
     8461 tcaggtgtta atttaaaagc ttatcataat attgctgtgt gtttagggac ctcacttggg
     8521 ggaaagagtg ctggtcaaaa tgccttgtat caatttgaag aaggagagcg tcaagtagat
     8581 gctagtttat tagaaaaagc atctgtttac catattgctg atgaattgat ggcttatcat
     8641 gatattgtgg gagcttcgta tgttatttca accgcctgtt ctgcaagtaa taatgccgta
     8701 atattaggaa cacaattact tcaagatggc gattgtgatt tagctatttg tggtggctgt
     8761 gatgagttaa gtgatatttc tttagcaggc ttcacatcac taggagctat taatacagaa
     8821 atggcatgtc agccctattc ttctggaaaa ggaatcaatt tgggtgaggg cgctggtttt
     8881 gttgttcttg tcaaagatca gtccttagct aaatatggaa aaattatcgg tggtcttatt
     8941 acttcagatg gttatcatat aacagcacct aagccaacag gtgaaggggc ggcacagatt
     9001 gcaaagcagc tagtgactca agcaggtatt gactacagtg agattgacta tattaacggt
     9061 cacggtacag gtactcaagc taatgataaa atggaaaaaa atatgtatgg taagtttttc
     9121 ccgacaacga cattgatcag cagtaccaag gggcaaacgg gtcatactct aggggctgca
     9181 ggtattatcg aattgattaa ttgtttagcg gcaatagagg aacagactgt accagcaact
     9241 aaaaatgaga ttgggataga aggttttcca gaaaattttg tctatcatca aaagagagaa
     9301 tacccaataa gaaatgcttt aaatttttcg tttgcttttg gtggaaataa tagtggtgtc
     9361 ttattgtcat ctttagattc acctctagaa acattacctg ctagagaaaa tcttaaaatg
     9421 gctatcttat catctgttgc ttccatttct aagaatgaat cactttctat aacctatgaa
     9481 aaagttgcta gtaatttcaa cgactttgaa gcattacgct ttaaaggggc tagaccaccc
     9541 aaaactgtca acccagcaca atttaggaaa atggatgatt tttccaaaat ggttgccgta
     9601 acaacagctc aagcactaat agaaagcaat attaatctaa aaaaacaaga tacttcaaaa
     9661 gtaggaattg tatttacaac actttctgga ccagttgagg ttgttgaagg tattgaaaag
     9721 caaatcacaa cagaaggata tgcacatgtt tctgcttcac gattcccgtt tacagtaatg
     9781 aatgcagcag ctggtatgct ttctatcatt tttaaaataa caggtccttt atctgtcatt
     9841 tcgacaaata gtggagcgct tgatggtata caatatgcca aggaaatgat gcgtaacgat
     9901 aatctagact atgtgattct tgtttctgct aatcagtgga cagacatgag ttttatgtgg
     9961 tggcaacaat taaactatga tagtcaaatg tttgtcggtt ctgattattg ttcagcacaa
    10021 gtcctctctc gtcaagcatt ggataattct cctataatat taggtagtaa acaattaaaa
    10081 tatagccata aaacattcac agatgtgatg actatttttg atgctgcgct tcaaaattta
    10141 ttatcagact taggactaac cataaaagat atcaaaggtt tcgtttggaa tgagcggaag
    10201 aaggcagtta gttcagatta tgatttctta gcgaacttgt ctgagtatta taatatgcca
    10261 aaccttgctt ctggtcagtt tggattttca tctaatggtg ctggtgaaga actggactat
    10321 actgttaatg aaagtataga aaagggctat tatttagtcc tatcttattc gatcttcggt
    10381 ggtatctctt ttgctattat tgaaaaaagg tgataaaatg agtaaatttt tatttgctcc
    10441 tttcatgatg aatttaggag agagccaaag attatcaaaa ctggctaatt gtttatatga
    10501 acaaggacat gatattcata tccttggaga caattattat ccatttttat ttaataatga
    10561 tgcttatcgc tatcaccact gtgtagaaga taaatctgtt tataatagta agcgatatga
    10621 tgctttcttt tctctttcaa cagactttaa ttttttatca gaagaagaaa ttgaacgcat
    10681 ctgttctatt gaaagagact tgttacgcca agaaaaattt gatgctgtat tgacaggtta
    10741 ccgtctatct attgttacaa gttgtcgttt agaaagcata ccattaattt ggattatttc
    10801 aggggcaacg catatcagtg aaattgttga aaactcagaa ggaatattgc ctaatgggaa
    10861 gataagtaaa gctagtaagc ctcaaacaaa ggattttatc aaaagagtaa tcactactta
    10921 ttcaacaaat gttaaaacgt ggaataacta tattaaaaaa tatggtggca aaccttttaa
    10981 taatgcttta gaattattta ctggtgatct aaacttggta acagattact ctttgtttta
    11041 tgaatttgat aaagattcgt cctataaaac gataggacct attttgatag ataatgtagg
    11101 tttttcaaaa tgcagccaaa ttaatcaaga caataagact gtactgctca gctttggtac
    11161 ttcatttaaa cgagattggg tggaatcttt tttaaagaca ttaccaaggc attatcatta
    11221 tttactgaca agctgtggtg aggctataaa cattccagga agttatattg agatagttga
    11281 ttttattgat tttaaaacca tagataaaga gatttatttc gctattatac acggtgggca
    11341 aggaacagtt tatgcgatgg ctgcacaagg tattcccttt ataggtatcc ctttctttaa
    11401 cgaacaattt tggaatatta agaagttcag taaagaaaag tgcgcttatt tggtaaagaa
    11461 acctgatgta agagagatac gtaaagcaat tagctgtctt gaaagagact atgatatcta
    11521 taaaagttat atgataagac taagccaagg aattaaagaa gaagcagagg catctttaca
    11581 aaaagcggag gcagaaattg aatcctttat cagaaaaaga caaagttaac tatattatga
    11641 tttataaaaa taggcagtct acattggaga ataatttatc tggcgatcgg ttgatgagat
    11701 atcaacaagc aaatagacag gtaagcaaag ataaactgtt aacagggagt tattttgtgt
    11761 accaatgctt aaaaaagtta ggttttacca ataagatttg tcaggaatct ttttctagtg
    11821 ttagtagtta tttaattggt ttgccaaagg ggaaaattag ttactctaat tctggtgact
    11881 atcatattct aacctatgct cccagtggtt caactggggt tgatattgag aaatacaaag
    11941 atagatcaga acaaacctac caaaactacc taggagaatc agttagtagt gatatggatt
    12001 ctaaattatt attttataag gcatggttac aaaaagaaat ttcctataaa tgtgggaaat
    12061 ctatagatat tacctatcaa acaatgatag atggctatat ttatggttat gcttttgata
    12121 atacttttaa agtagaagcg gtttacttaa aagaattatt accagataac taactcgcaa
    12181 catgttgttc ctacatatgc atctataaaa agcactttga agtatttcaa gggcttttta
    12241 taataccttg ggtagtcttt aacaattatg gttagagttg atatgataaa ctgatcattt
    12301 ccacatttta tgagaaaatc agaaaataga ggtgtttttt tgtgataata attagaaata
    12361 ataaaaactg aaatattttc taatgtaaaa aaagataaaa gaacttattg ctgaaaacga
    12421 ttttaactta acatttgcca ttgacaagta cttagaggag tgctaaaatt tcgttagctg
    12481 gttaacaaac cggttaagta atgtgaggtt cttttatgag tgaattcctt tcagccaaac
    12541 ctattggtca taatgattca tctaatacta ttttaagaat tatagagaat acgaaatata
    12601 ttcatcacta tgatggaaaa gttgttgtcg aaattcctcc tcaaaaagag cctatcaaag
    12661 tcttttttat ggattcacgt cttcattatt tagaattttc agacatatgt cggtatgtaa
    12721 ctgttgtttt gaaaaatagt accgttgata atataaccaa tttagggcta gataatcata
    12781 ttgtttatga caatggtgat gatatggttg atccgttgcc gagtattcag catggattat
    12841 ttcttaatga tgattaaaga tgtagacaaa aatttagaaa cgaggaagcc atgacatttt
    12901 ataaaaagac ggacaaattt ggatttagga aaagtaaagt gtgccgcagc ctatgcggtg
    12961 ctttattggg tacagtagca gttgttagtc tagctacagc aagtacagag attcacgctg
    13021 atgaagcaac aacctcacct accactgtaa cgaaagtacc tcagccagtt caagcagaca
    13081 caacagctct aaatacctct aagactcaca gtacacaagc aactacaact cctgttgaag
    13141 ctaaggagaa taaggttgtc aaaagtgaaa ctgttcaatc tgagagccga gtaatgccta
    13201 gagataaggt agtagaaagg ccagaaacag taaaagctag tgttaactca gacgttagcc
    13261 aacctattac aaccacaccc ccaacaatta atgaaaaaac agttgagatt cctaacttag
    13321 ctcaagatac taagaaggta gctccaaaag taactgttac accagaagtt gttaatcagc
    13381 caaagcagtt gcctaaatac gagtaagaaa actcagagaa aggcaataag agtacagaag
    13441 caactaaggc agttactgtg actaaaccac agggggcggt tgctgagaaa gctactccgg
    13501 ctgtgccaaa gcctcaaaaa gtaagagtaa ttgttgaatt taataaggaa tctatcttag
    13561 attatgcgac agaacagaaa aaacagttgc acagttaaat caagctgatg ttgagaaaaa
    13621 attacaaagc atcaagcaag agcaagataa agttcttaag aatattgaaa aaagtgttca
    13681 ctttgattca agcaaagtga agcgttacga tgctattatt aatggtgtcg cacttgatat
    13741 tcaagctcaa gaaattgaaa aattaaaaac gattgctgat gttagaaggg tctatgtctc
    13801 acaagaatat gttcagacaa aacctttact atcttcctca gggcaactta ttggtttgcc
    13861 agaagtttgg aacaactccc agtataaagg tgagggaact gttgttgcag ttattgattc
    13921 aggggttgat ttcaaacacc aagcactaaa aatcaaagaa ccaaatcgtg ctaagtataa
    13981 taaaactagt attgaaaaac tgattcatga aaagaatctc aaaggaaaat tctattccga
    14041 aaaagtacct tatggttata actattatga ctataatgat aacctcaagg atagctacgg
    14101 tgtaatgcat ggtatgcatg taacaggtat cgtaggagct aatgatgata atcaaaaatt
    14161 gtatggtgtt gcaccaaatg ctcaaatttt agcgatgaag gtattttcag atgaccaaca
    14221 aaatcccaca accttcacag atgtgtggct aaaagctcta gacgatgcta ttttactaaa
    14281 agctgatgtt gttaatatga gtcttggaac acctgctgga tttgtacatg aggggaaaga
    14341 ctatcctgaa cttgaagtta ttgcaagagc gtgtaaggca ggaatcgtca ttgcagtagc
    14401 agcaggtaat gaaggaaata tcactgacgg taatacttat ggtgttaagc cgctagcaga
    14461 aaattatgat acagccttga ttgccaaccc agccctcgat gataatacct tggctgttgc
    14521 ctctatggag aaccttaaaa agcatgctca tgttttgaaa tttaaagata aaaaatctgg
    14581 aacagaagtt acagaagtca ttaatcttca tgttgcccca aacgcttcaa aaactattat
    14641 tggattggct gtagatttgg gagcagaagc tccttcggaa ttgtcaaaac attttgattt
    14701 aagtggtaaa atcgccatgc ttgaaattcc agaagataat aaatctaatg gatttttaga
    14761 aaaagtacag gcgattacta agttaaatcc agctgctatc ttgctttaca ataatgcgaa
    14821 ggttaaagat gatttaggca gtcaattatt agttgaatca gaagcagcta aatttaatat
    14881 tgctcgcatt accagaagca cgtataacaa tatcaaaaat aatagcaata aaattattac
    14941 tattttaaca gagcgtcaag ctattgataa tagtttagca ggacaattat cttcttattc
    15001 ttcttgggga cctacaccag acttacgtct aaaacctgag attacagctc caggtggtca
    15061 tattttctca actgtggagg ataatcaata tgctgataaa tcaggaacat caatggctgc
    15121 acctcaggta gctggggcag ctgcagtttt aaaacaatac attacagata aaaaaattcc
    15181 tgttgataat gctgctgatt ttattaaatt actcttgatg aacactgcac agccaattat
    15241 caataaacaa tcaaaagatg gcaaaacacc ttactttgtg cgccaacaag gtagcggagc
    15301 aatgaatctt gccaaagctc ttgttacgac agtcgttgca actgtaacag ggactaacga
    15361 taataatgca gatggtaagt tggagttacg tgaattaaaa gagaaaaaat ttaaagcacg
    15421 cattctactt cgtaactttg gaaaaactaa taaaacgtat atcatctcat cagaagcgat
    15481 tgcggaccca gttgatgaaa aaggatttcg tacgcaaaat tcagaacacc tagtatcaaa
    15541 aaaagctgat gcagttacaa ggaaagttac tgtagaagca ggtaagacat tagctgttga
    15601 ccttgatgtg gattattctg atgcagaggc tttaactcgc aataatttct tggaaggtta
    15661 tcttaactta aaagatactg aaggtgtagc cgatttacac ttaccatttt taggttttta
    15721 cggttcatgg acagagcaaa aagctattga tgcttttgaa ggcatttctg aaattggtaa
    15781 cggtgacaaa aaacgtcgcg ttcaattcta tgttaataaa gaaaccaaca agacctcatc
    15841 aacctttaca actaacggca tgttgagtct tccaatctat aacaatacag tatttttctc
    15901 tcctaatagt cctttctatg acaaagcagg agttcgtatt gcagctttgc gtaatatgga
    15961 gtatgttcaa tattctatta tagatccaga taccaataaa gaagttcgtg tacttggtag
    16021 atcacacgac gttagaaaac tttatcgcct agattatcgt aatagttttg ccatgatgcc
    16081 agattctatt tgggatggta agattaaaga ttaaattgca aaaggggata aacaatacat
    16141 ttatcaaatt aaagtacagt tgaataataa aggtgtcggt ggcgatggtg tccaaattta
    16201 tcaatactat atcaaaatgg acaacaataa accttactta agtcccaaag ataagactac
    16261 tgtagagaag ttagaagatc gctggaaaaa aattactttc aaagttcagg atactggcat
    16321 tggtttgaaa gacgtttatc ttcaatctgt taagtatgtt ggtggtggca ataataattt
    16381 agaccttatc acacctccag gatttaaaaa agaagataaa aaagttgaaa aaccaaaatt
    16441 agaccgtcca ccaggaattg atttaccagc accaacttca atgagaagtt ttgattattc
    16501 aaccccaccg ggaactaagc caagcaaacc caaagatagt ttatcaactc ctccaggttt
    16561 cccagattta aacacgccgc cggatgaagc caccaaagga tagtaaaaaa gacgctattg
    16621 aagataaatc aggagcaatt aaatatgcta agtctcttca acttagcttt gttgatgacc
    16681 ctattttagc tagcaaagta aatggcaaaa tattacaagt cgaatctgat ggcaaattag
    16741 tcattcctag aaatgctttg tcagctaatc aatttgatga cactagtctt aaaatttatc
    16801 gtaataataa tcgcaataaa gaaattacta tcacaacaga ttattttgca gatacaaaat
    16861 atgtcaatat cacagcggtt gactatttga gcaatactac ttttgagcaa ttagctactg
    16921 gtgaaacagt agattaccat gccattgtat tttcaagctt tgctgctatt aaagacaagg
    16981 gtggtaagat ttatgttaac gataaattgc aagaaacttc tcgtatagcg cttaaagata
    17041 aatctgttaa gattggtatt gaattaccaa atgatgtcag acatattgat agtttatctg
    17101 ttcgtcgttt gaatgaggtt aaaactgttg ataatatctt gaaaaatgat gaacaagaca
    17161 ttaatctcag caaaacttac caattaaaat acaacccgac aaatcgtcgt ctagagttta
    17221 ctattaataa cattaactca agttcagaaa tcatgaccac tttcaaagat ggaaagatgc
    17281 cagaattggt tgaacaaaaa gatgtttctt tggatataaa cgatatggac atgagtaagt
    17341 ttaaaactat tcgacttgga cgaaaggatt ctgaatttaa gggacaactt attgcaaaaa
    17401 ctggaacagt tgaattagat atgtttttca aacaatctca agacccagct tcaattatta
    17461 aaaaaatata ccttatccaa aatggtgttc caaatgaatt gaaaaaattt gactctagtt
    17521 ttggtttaac tgaaagtcag atagatggat actatattta taaagatgca attaacctta
    17581 aatttaaatt aaccagtggt gcaagtctta aagttgttta taaagggcaa gaagatccat
    17641 atagtcatca gaaagaagat atgactaaaa aaggtgaaca gctcagtcat tcaactcaag
    17701 ccaatgaaaa tacagcaaaa gtaacctttg ctaatattga ctggtcacat tatagtaagg
    17761 ttactgtgaa tggaaaagaa gttggtaaag gtagtgagtt acctttaact aaaggatgga
    17821 caacatttgt attacataaa acagaaaatt cattaaatgt taaaagtttg attatggaga
    17881 cgggtagtgt aagtaagaaa gttcaacaac ttcctttaag tcctagatta tctaaaaata
    17941 agcatatgag ggatatgcta cttactatgc aaaaagattc agcgtattac gaaacaagtg
    18001 acagtctagt ccttcgaatt aatctcactg cagatactaa acttaatttt aatgctgtta
    18061 aaggagcgag tgctcttact gaaaatatga tgatgagaca gtttgcagtt gctggaccac
    18121 aagatgatcc tgttagtgaa cataaatacc catcagtatt tctcttaact cctgccttat
    18181 tggaaactgc tagtgaggca actctaaatg gtaaggaaat cacagcatct ggtattatcg
    18241 gtcacatcaa ggatggtgat aaaagcaagc atgttgaagt caaaatggtg aatgaaaatg
    18301 gagacatgct aggaacccct gttattattc aaggtaaaga cttgactaat cgaacaaaac
    18361 cattaatgag tggacgtaga gtactttatg ccggtaaaca atatgagttc cgggctaaat
    18421 taccacttag tcgttttaac acttggatta gggttgaagt ggtaacagaa gcaggagaga
    18481 aagcaagtat tgttcgtcgc atgttctttg accaatcagt tccagagctt aacacagcag
    18541 ttgctaaacg tgatttgact tctgatactg ctcttatcca catcgttgcc aaagatgact
    18601 ctctaaaact aaaattatat caagatgatt cattacttga atctgttgat aaaaccggtc
    18661 tttatagttt tagaaatggt gtagaaatca ctaaagatat gacagtacca ctagaatttg
    18721 gagataatat tattaagtta tctgctgttg acttatcaaa ttatcgtcgt aatgagaccc
    18781 ttcatatcta tagaaaccgt tttgatgtta aagcaagcca aatgacagct gacaaaggag
    18841 ctaaagtaac tgtggatatg ttgatgaagc acttagttgt tccagaaatg gcaggagctt
    18901 atacattaac aatcgacgaa gctccaaaca caaatgaatc aggaatgtta acaaacgcta
    18961 aagtatcgat tcattatgta aatggtggtg ttgataaagt tgatgttccg attaaagtag
    19021 ttgacttaga agctattcgt aaagctgaag aagcacataa agctgacgaa gcacgtaaag
    19081 ctgaagaagc acgtaaagct gaagaagcac gtaaagctga agaagcacat aaagctgaag
    19141 aagtacgtaa agctgaagaa gcacataaag tcgaagaagc acgtaaagct gaagagggac
    19201 ataaaaccca agaagcacct atagttgaag aaggctacaa agttaataac gttcatcaaa
    19261 ctgatactac agttaaagcg tctgatttac caaagactaa gacagtttcc gcagttcata
    19321 tggctagaac agacaataaa cagataactt cacatcagac acatgttgaa aaacaaatta
    19381 aaaatacatt gccatccact ggtgacagca aacgtggtta ttatatcact ggaatggcta
    19441 tcgttatgct gagtgtatta tttagtttag ctaaaaagtt taaaagcaaa tattaatgat
    19501 aaaataaaat ctatttagca acattaattg tgatggataa aaggttttat ctcttaaatc
    19561 aaaagtagta gctcattagc tactactttt ttgtataagt gtaaaaaagt gaattcttaa
    19621 caagaattta acaccctact tgctatacta aaagtgaata agaaagagag gtgtcattta
    19681 tgcaaagaaa atcctattta aaatccatga gtgttcttac tttaacagct tgtcttatat
    19741 caggatatgt ggttaaagat attgctatgt tacatgcagt atctgccagt gagaagaaag
    19801 caaataatgt cagtccgaga gaaaatctct acagggctgt caatgataat tggctagcca
    19861 atacaaaact caaacaaggg cagactagtg ttaatagttt ttcagaaatt gaggataaat
    19921 taaagcaact gttagtgtct gatatggcta aaatggcctc aggaaagatt gaaacaacca
    19981 atgatgaaca gaaaaaaatg gttgcatact ataaacaagg tatggacttt aaaacaagag
    20041 ataaaaatgg tctcaaacct ctaaaaccag ttttacaaaa acttgaagca gtctcttcaa
    20101 tgaaagactt tcaaagtttg gcccatgatt ttgtgatgag tggttttgtt ttaccatttg
    20161 gtttgactgt ggaaaccaat gctcgagata atagccaaaa gcaattggtg cttcgtcaag
    20221 cacccgcatt acttgaatca cctgaccaat ataagaaggg caataaagaa ggtgaggcta
    20281 aattatcagc ttaccgtact tcagcaatgg ctttgcttaa acaagctgga aaaagtaaca
    20341 ttgaagatag aaaactagtt aaacaagcta tagcatttga tagactctta tcagaaaaaa
    20401 cgcaagttga tcaaagtaaa atcacagctg aaagtgagac agctgcgggg cgatataacc
    20461 ctgaaagtat ggaaacggtt cacaattacg ccaaggaatt tgactttaaa gaattgattg
    20521 aaaaactagt tgggccaacg aataaggcag tcaatgtaga agataaaact tattttaaac
    20581 aggttaatga tgttataaat agtaaacaat tagccaatat gaaagcatgg atgatgattt
    20641 ctatgctagt tgatcaatca gattttctag gagaacaaaa tcgtcaagca gcgagtgctt
    20701 ttaagaatgt tgcgtctggt ttgactcaga ttgaattgaa agaaaaaatg cttacaccca
    20761 attagaatat acttttggcc atattttggg tgattattat ggtaagaaat attttggtga
    20821 ggcagctaaa aaagacgtcg aacatatggc taagaaaatc attaatgtct ataaaacacg
    20881 gttaaaaaac aacacttggt tatcagaaaa tacaaaagca atggccatta agaaacttga
    20941 taacatgaga ttaatgattg gctatccaga agattatcct gatctttatc gtcagtacca
    21001 atttgatagt aaagcaagct tctttgaaaa caatgataac tacagaaaat tatcgaacaa
    21061 gaaaacattt gaagaattta accagtctaa tcaacgtgaa cattggcaaa tgagtgccaa
    21121 tgctgtaaat gcttataatg atcctaatac caattccata gtctttccag cagcgatttt
    21181 tcaatcacca ctctacgata aaactaaaac agttagtcaa aattatggag ctatcggagc
    21241 aattattggt catgaaattt cacactcatt tgatattaat ggtatgaaat atgacgagaa
    21301 agggaatctt cacgattggt ggactaaaga agatttaaaa cattataaga aaaaaacaca
    21361 agctatgatt gaccaatggg atggccttaa agcagatggc ggtaaagttg atggtaaatt
    21421 aactttagca gaaaatattg cagataatgg tggtgttatg gcatctctag aagctcttaa
    21481 gactgaaaaa atccaaacta taaagaattt tttgaatcat gggcaagtat ttggcgtcaa
    21541 aaagcaacca aagaacaaag taagtcctca attcagtcag atgttcatgc accatatgaa
    21601 ttgagagcta acatcccagt acgtaatttc caagaatttt atgatgcctt tggtgttaaa
    21661 aaaggcgatt caatgtatct aaaaccagaa aaacgtttga cactttggta aagctatgtt
    21721 ttccagacaa taaggagaat gcatgaagaa tataacaaag ctatcaactg ttgctttaag
    21781 cctactactt tgtacggcgt gtgctgcatc aaacacgtct acatctaaaa cacagtctca
    21841 tcatcctaaa caaactaaac tcacagataa gcaaaaagaa gaacccaaaa acaaagaagc
    21901 tgctgatcaa gagatgcatc cccaaggcgc tgttgatttg acaaaatata aggcaaaacc
    21961 ggtcaaagat tatggtaaaa aaatcgatgt tggtgatggc aagaaaatga acatttatga
    22021 aactggtcag ggaaaaattc caattgtttt tattcctggt caagctgaga tttcgccacg
    22081 ctatgcttat aagaatttaa ttgagcggtt aagtaaaaaa tataaaattt atacagtaga
    22141 accattaggc tatggtctca gtgatattcc tactaaacca cgtacgctag aaaacattac
    22201 aaaagaaatt cacacaggcc taaataaaat tggagttaaa aatttctacc ttgcagctca
    22261 ctcacttggc ggtatgtatt ctttaaatta tgctaaaaat tatccagaag aagtcagagg
    22321 atttattggt atggatacca gtacaccttg gatggaaggg gaacaaaaaa caaagtatga
    22381 ccctgaaagt gctaaatggg caatgaaatt accagatgtt gatgataaga ctaacgaaca
    22441 gtatctgtca attgcgaaaa aaattaacgg caataagtct caacgagatg aagatgagcg
    22501 cttaggtgat aatcttggta aattgaagaa tgtaaaattc cctaaaggga tgccaactaa
    22561 gtattttctt gctgatcaat ctgtggaggg aatcaagcta cgtcaagcag attttaaagg
    22621 tttgaaaaat tgggatcagc agcatattga tttatctgaa gatccaaaag atgcttccgt
    22681 tgaaattctt aaaggtagtc acaatatttt ccaaacacaa tatgaacata tggctaaagc
    22741 tattgatgga tttatccaat cccatcaaaa gtaatcgttg aatgaaatac ctaagtcaag
    22801 gagaaaaaat gaataaaata acgaaattat caaccgttgc cctcgctttg atgctttgcg
    22861 ttggatgttc tgccaataaa gataatcaaa aaactaaaac tgagcagcct aagcaagaaa
    22921 agtcaatcgc taagcaaaaa caagagagag aagcagaaaa agctatgatt gctaaaggtg
    22981 ctattgacct aactaaatat aaagcgaaac caattgaaga ttacggacaa aaaattgatg
    23041 tcggtgacgg taaaaaaatg aacatatata caatgggtca agggaaaatt ccgctagtag
    23101 tcttccctgg tcaagctgaa atttcaccac gttatgctta caaaaacttg cttgatagat
    23161 tagggaaaaa atataaaatt tatacggttg aaccgctcgg ttatgggctt agtgatatga
    23221 cagataagcc acgtacgatt gaaaatatta ccaaagaaag tcggtgtcaa aaatttctat
    23281 cttatggctc actcgttagg tggcatatac ggtctaaatt atgctaagac atattctaac
    23341 gatgtcaaag gtttcattgg catggataca agcacaccaa atatggaagg tgatggcaca
    23401 actggagaat cttcagcttc aaccttaaaa tgggcagaat ccattccaga agttgataag
    23461 aaaaccaatg aacaatactt atcaatcggt gctaaaaaat caggtaataa aacagagcgc
    23521 gatgaagata ggcgtgttag tgacaatctt cataaaatgg aaaaagttaa gttccctaaa
    23581 ggtatgccag ctaagtattt cttagctaat gagtcagcag aagatataaa aatgcgtaaa
    23641 aaaatattcc ctaagcttaa aagctgggaa caacaacata ttgacttatc tgaagatcca
    23701 aaagacgttt ctgttgagac actagatgct agccacttga tttatcatac gcaatataaa
    23761 catatggcga aggctattga cgagtggatt caatcgcatg aaaaataatt attcccccca
    23821 attattttac taaggagaaa agatgaataa aataacgaca ttatcaacca tcgccctgac
    23881 tttaatgctt tgcgttggat gttctgccaa taaagataat caaaaaacta aaactgagga
    23941 tcctaagcaa gaaaacgtaa ctgcaaatcc gaaggaagac aaggcaacag cagccaagcg
    24001 aatgaagtta acgaaagaac ttgaaaaata taataaaggc acagagagct ttattagtaa
    24061 caaacaatat caaaaatata tcaaaacacc tatcaaagaa tatggtcaaa aggttgaagt
    24121 caaaggcaag aagatgaatg tctatactgt tggagaaggt aaagtaccaa ttgtctttat
    24181 tcctggtcaa gggacggtga ctgccaaaca tcagtaccat aatttgatta gtaatttaag
    24241 taaaactcat aaggttgtgg tagttgagcc atttggttca ggtttaagtg atgtcattga
    24301 ccaaccacga aatctagcta atattacctc agatatccac gaagctttac aaaaagttgg
    24361 tattaccgga aaatatgtaa ttgcctctca ttcaattggt ggtgtttatg cccttaaata
    24421 catcagtact tatccaaagg aagtgctagg attaataggt ttagatacat caacgccggg
    24481 tatggaaggt ggtaagcaag ttgatttcgc tgctccagtg ttaaaagaat tacctaaaat
    24541 tcctaaagta tccgacgata ttaatgcaca attttttgct attggacata agatacttaa
    24601 taattctaat atgaaagaag aagcgaagaa ttcttctaat atgattaacg aaagtgccaa
    24661 ctataagatt ccaaagggaa ttcctgctat gtatttatta gcacgtgaaa gtgacttagg
    24721 gcttcaatta cgtagtttag atataccaat taaaggcagt tggcaagagc aacataagga
    24781 gttatcagaa gatccaaaag aagtctctat tcatacacta gacggaactc accaaattta
    24841 tgatactaac cttaaagaag taattgatta tactaaccaa tttatgaaaa catttgagaa
    24901 ataattgttt ttagaaacag tgctataaaa tcactaattg atagataacg tagaagtcga
    24961 tagagaatat ctaacgactt ctatcgttat tctagagatt attaagatag aaaaaggagg
    25021 gaaaatagtg aacctatgga cattaagttt agcttatttg aaacgtcaaa aaatgaaaac
    25081 agtaaccttg ttcttagttt ttctaactat aggcacatgt ttaatcagtc tgatgagtat
    25141 tcagcattct ttggaaaaaa atatcctaac aaaacaaggg aaaagcattt atttaacctc
    25201 caaagaaaag gcttattggc ctgaacaagc atacgaagct ttgaaaaaag caaaaatggt
    25261 tgagtcagta gaggcaagtt taagcattga tgttggtagc cgtttaaaat ctgtatcacc
    25321 atataactct tctaaagaag agaatcaagt tacattagca ggttatcaaa gtacagaaga
    25381 tttacgagct tttcagacaa aagcattggt cttaaagaaa ggaagccatt tagcggccga
    25441 taatacaaaa caagtactag ttccattgaa gttagcacaa aagaatcatt taagtgtggg
    25501 taacaaattg agattaggca aagaaaatgt tactattgca ggaatttatg atgcgaatag
    25561 tgctaagtct aaaaatactt ttaatcccaa tattgataat acattgattg ctcaggcaac
    25621 attggttagg aagattagta aacaaaaagg ttatcagacg gttgcagtgc gcttaagtga
    25681 taaacgttta gtggatacag ttatccaaaa catcaaacaa tggccgcttg atttcgggaa
    25741 attagatgtt caaacagcta aagagtttta tggagattct tatcgtaata tcgagacatt
    25801 gcatcgtcta gtgggacgaa tcattcttat tgttagtctc gtggcgatgg caatattagt
    25861 tgtcatgcta acattttgga ttaataaccg cattaaagag acgggaattt tactcgctat
    25921 cggaaaaact aagtttgaaa tcataggtca ttatctgata gaagtactgt tagtagcagg
    25981 tgctgccttc acactatcta ttattggtgg tgtctttctt ggaaaaacat ttgcagctgg
    26041 ccttttatca caagttaatg gtggtgtgag ttcacaaatt gtacaaaata gtagtctaat
    26101 tattgataga attgataacc tcgctgtttc tgttggagta atggatgtct ttagactcta
    26161 tgctcagggt gctttaattt gcttatttgc agttgtatta tctagttatt ctattcttaa
    26221 actacaacca aagcaaatat tgtcacgaat gtcataggag gttaatatga atttatttaa
    26281 acgttccttt ttatatgtta gccgtaaaaa gcgtaagagt atcactttat tcgtatgttt
    26341 atggctagtt gcttctacat taattagcgg tattgcggta aaaaatgctg gtcttactgc
    26401 taagaagaca ttttcaaggc aaactggatc aattttgcat atttcatcgg atagtactga
    26461 ccttgttggt gatggttacg gatctggaga aatccctgaa aaagcaatag ttaacatagc
    26521 aagtaatcca aatgttaagc gcgttaataa taatttgatg gcttatgctg gactaacctc
    26581 ggaaaaaatg gttacacgac caaatgataa agaacaatat aaagaacaag ttttacaagt
    26641 tcatggtaat agttactccg atacagatcc taaatacaca gcggggatga tttctcttaa
    26701 gggagggcga catatcacgc cgaaagatca gcgagttata attgttcatc aggcttttgc
    26761 gaagacaaat catcttaaag ttggcgataa gttaatgttt tcaaaagacc ccgttagaaa
    26821 cagtaaggat aaaacattag ttgaagcaac tattgttggc atatataaag gtaaaacggt
    26881 acaaaaatca aattaccctt aggagatgct agaaaataca gccttttcag atacgcaatt
    26941 gattaaggat ttatacggct attctaaagg taaggccttt tatcgccaaa caactgtata
    27001 tcctaaaaag aatgctgata tagaaactct taaaagtttt atcaaaaaac aatcctattg
    27061 attggaataa atatcaactg acaattacag caaccttgct aacaagttat gcaaaaagta
    27121 tagatgtctt gaatcaatta attacttctc tacaaggcgg catgttagta gtatctatta
    27181 tcctagtgat gatggctctg ctattctgga ttggtggtag aacccatgag acaggtattt
    27241 tattagctat tggtaagtct aaagcaatta tcattgtcag tacgcagtag aagtactaat
    27301 gattgctatt ccagcctttg ctttatcagt acttatcagt caatttattg gtcaaaaatt
    27361 agcagactcc ttagtgtatc aggcctctag aactgtcaga gcagacttta tgaaagggtt
    27421 tgggaatatg cagcttgggg ctgatagtga tacagatatg tttatgcaaa cgattaagac
    27481 gttaaatgta caagtgaagc ctgaggaagt ctggttagta attctagttg gtttagcaat
    27541 cgtcttattt gctattatta ctactagttt aattattgct cgctacaaac caaggggaat
    27601 cttatcaaaa ctgtcctaaa gtgccataag tataccgacc aatagtgaca tctatattaa
    27661 cggttaaaat gaatatgctc tgctattgaa tacatagctc aagaaagaga aatctatgct
    27721 cgaattaaaa aatattgctt atcgttataa ggggaacgac aataaaacat tagaaaatat
    27781 caattatagt tttcagtcag gggtatttta tactatatta gggaattctg gttcagggaa
    27841 aacaaccctt ctatccttga tggcaggtct tgattcgcct actgagggac aagtattatt
    27901 taacaaaaaa gatattaaag aagcaggtta tgctcagcat cgcaaaaaga atatagcttt
    27961 ggtctttcaa aattataacc ttttagacta tctaacccca ttggaaaatg ttcagttggt
    28021 aaaacctact gcagataaac aattattgtt ggatttaggt ttaaaagaag atatgttaac
    28081 gcgaaatatc ttgagattat caggagggca acaacaacgt gtagctattg cgagagcttt
    28141 ggttgtagga acaccagcta ttttacttga tgaacctacg ggaaatttag attttgacat
    28201 tgcgcgtgat attacgatgc gtcttaaaga ttttgcgcat aaggaaaaac gatgtgttat
    28261 tatggtgaca catagtagag agatcgctca tatggctgat actgctttac aattgatagg
    28321 agataactta aaagaattat caaaagaaag tatagcacag tctatattac aacaaaggaa
    28381 tttataataa aaaaatacct ccctttctta aataataaaa gaggaggtat tttttagaaa
    28441 tggttattta ttatcagaaa aggctcgctc tgccatttgt tgaattttaa ttgtagagtt
    28501 attctgataa acgacaccat taaccagtgc accatgcgca tataaacctg gcattggacc
    28561 atattttgga gacaaaactt gtgcattttc agttaaaata gtaatccctc cttgatcgtc
    28621 aatttgacaa aattcttgat tgatgagcga ttttagaagt ggattggctt ttgtccagtt
    28681 tttagtgttt aactggtaac cagtagcgtt aatgacgcga tcgacagata tatgattttg
    28741 tttttctttt tctattgaaa atccttcgtc attttttgta attgtcttga cgttattgat
    28801 cagagaaaaa gtcccttttt gtgcagcttt caaaattact ttagcagatt catcaggcat
    28861 aggatttcgc aagttaataa tcgcttttcc atagagtgct ttatactctt tcttatctga
    28921 taatgacatg agtggccaaa ggtcagttaa ccagacagtg attcgtgaag ctaattgttg
    28981 taatagatag agttgcttag ggtatttaaa ggctaacttg agaccatcaa ttcctgggga
    29041 taaaaaagtt tctgttgtat ttttccagtt tttaaagcca agatgctcca attcgccaga
    29101 catcaaatca ttaaaatcgg caatagtgaa ggtttttgaa ggatgtgata gaaatgcttt
    29161 aaggttatca ttggtaaaaa agtgccaagt aagtggtaaa tcattaacaa tacggacgct
    29221 agggaagtag ttggttcttg aaaatgcttt aacagaagat tgtgtattga gtagcaacca
    29281 ttttataaca tcaattgcag ctagaccagt cccgatgata gcaactgttt ttcccttcca
    29341 attttcctga gccaagtatt tgataggata agggtctttg atataatcgg tcttcccttc
    29401 caaatgataa ggttctataa caggcagagc accacaagct aagtgtaatt catcaaatgt
    29461 gacaggaaaa agcttatctt gaatatagac ttgccattgt tgctcatctg ataaatagtt
    29521 tagtttatcc actcttgttt taataaccgt gacagggagt ttatctaaaa gtaacttagt
    29581 tctttctgaa aggtattgtc cgtaaagtgt ccttgataca taggttagat ttgtgtcata
    29641 gtaattttct tctaaccaat ccatgaagtc agacatatgg cgataatcaa atgaaatatc
    29701 gttgattggg gaattgatta aagctgagtc aacgtcttct tgaaaaggaa ttcctcgtcc
    29761 aaagtggaca gtatcatcaa aacaaaaaat agctaactgt tttaactctt tctctttttt
    29821 acgagaaagt gctagtagaa cagctaaacc actaactccc atacctataa taccaattgt
    29881 ttttttcaca gaacaagcct ccttaccact atcatagcat aaattttgat ttaagttagg
    29941 ggatgaacaa agctattatt tgcagatttt ctaatttaac gagtaaatta aatttaaagt
    30001 aatattaaat taatataaga ttaaataaat gattgacatg cttaaataat aggcatatga
    30061 tatggttgtg acaaaaggag agcaattagc taaaacggat atcaatatgg tagacgcttg
    30121 tttaatgtaa ttagaaagag gtaaagctta tgaaaagatt acataaactg tttataaccg
    30181 taattgctac attaggtatg ttgggggtaa tgacctttgg tcttccaacg cagccgcaaa
    30241 acgtaacgcc gatagtacat gctgatgtca attcatctgt tgatacgagc caggaatttc
    30301 aaaataattt aaaaaatgct attggtaacc taccatttca atatgttaat ggtatttatg
    30361 aattaaataa taatcagaca aatttaaatg ctgatgtcaa tgttaaagcg tatgttcaaa
    30421 atacaattga caatcaacaa agactatcaa ctgctaatgc aatgcttgat agaaccattc
    30481 gtcaatatca aaatcgcaga gataccactc ttcccgatgc aaattggaaa ccattaggtt
    30541 ggcatcaagt agctactaat gaccattatg ggcatgcagt cgacaagggg catttaattg
    30601 cctatgcttt agctggaaat ttcaaaggtt gggatgcttc cgtgtcaaat cctcaaaatg
    30661 ttgtcacaca aacagctcat tccaaccaat caaatcaaaa aatcaatcgt ggacaaaatt
    30721 attatgaaag cttagttcgt aaggcggttg accaaaacaa acgtgttcgt taccgtgtaa
    30781 ctccattgta ccgtaatgat actgatttag ttccatttgc aatgcaccta gaagctaaat
    30841 cacaagatgg cacattagaa tttaatgttg ctattccaaa cacacaagca tcatacacta
    30901 tggattatgc aacaggagaa ataacactaa attaataaaa gagcaactaa ttgctctttt
    30961 tttgttgtca ttcttagtga ttaatagtgt taaatagaaa tttttggata cattacttag
    31021 tgttccgata tgatttgaca ccagaaataa ggacaggaat taaggtaaca aatacaattc
    31081 ccaagataat aagcgagaag tgctgcttca caaaaggaat attaccaaag agaaaaccag
    31141 aacctgttgc aataactgac caagataatg ctgcaatgaa ggcgcgtttg ataaatactt
    31201 ttggagggag ctgactaata cttgctacaa aaggaacaaa ggttctgata atagggatat
    31261 agcgccctaa aatgatggca gcagtacctt ttttctcaaa ataaagctca gcatctcgga
    31321 tatttttttc tttaatgaaa cgacgaaaga aaggatgttt gataaaacgg taaccaaatg
    31381 tacgaccaat caagaagttg caactatcac caataaatgc tgcaataaag aaaataataa
    31441 gaaaagttac aatattgaag gacatttttg gatttgcagc aagggctcca gcagcaaata
    31501 ataaactatc acctggtaaa aatggaaaga tgacagcccc agtttcaacg aaaataacga
    31561 ggaagagcaa tagataggtc caattaccaa cagtgttagc catagcatag atatgagtat
    31621 caatatgtaa aataaaatcg attaaaaaca taggagtctc ctttattgat caactaaagt
    31681 gcagagactt ggatgataac tatcttgttt ataaggcctc ttacaaagcc tattttatca
    31741 gaaaattttt aataagaggt aaaataagtt tacttattaa tttagaatta ttctaaagag
    31801 cttttttttg atttttatgt tagacttaat tataatgatt ataaataaga aataaaggag
    31861 cttaacaatg tcctatttaa cctttaaaca cgttgatttt aaaacggatg ataagttggt
    31921 tttaaatgat atcaactttg caattgatga aggtgatttt gtttctattg tagggccatc
    31981 tggaagtggt aagagtactg tattaaaatt agccagtggt ttaatgagtc ctactgcagg
    32041 tcatattttc tttgatggaa aagatttgaa ccaattagaa ccgattgaaa gtcgaaaaat
    32101 gatttcttac tgttttcaaa cgccacattt atttggtaat acagtggaag acaatattag
    32161 ttttccttac cacattcgac acgaaaaagt agattataga cgcgttgatg atttatttca
    32221 acgttttgaa atggaccagt cttacctgaa acaggatgtc aaaaaactat caggaggaga
    32281 aaagcaacgt attgctctga ttaggcaatt attgtttgaa cctaaagttt tactcttaga
    32341 tgaagttact tcggctttag acaatcataa taaagctatt gttgaaaagg ttattaaatc
    32401 attacatgac aaaggaataa cgattttgtg gattacgcat gatgaggaac agagtcgtcg
    32461 atttgccaat aaagtcttaa aagttgtcaa tggtagtatt gaagcaatgg aggttataaa
    32521 atgagcggtg caaattctat ttctttaatg tcattactaa tggcatcgtc attagttttg
    32581 attactttat ttttttctta ctggcaaaag ttaaacctag aaaaagaagt gattattagc
    32641 gctatccgag cagtgattca attattagca gtgggttttt tgctagacta tatatttggt
    32701 tatcaaaatc ctattttcac agctctttta atgctattca tgattattaa tgcttcttat
    32761 aacgcagcta agcgtggtaa agggattaat aaagggtttg ttatttcatt tatagctatt
    32821 gggtcaggga ctataatcac tttatctgtc ttgatttttt caggaatttt aaaatttgtt
    32881 ccgaatcaaa tgattcctgt aggtggtatg attattagta attctatggt agcaatcggt
    32941 ttgtgctata aacaattatt gtccgaattt agaagtaaac aagaggaagt ggagacaaaa
    33001 ttagctttag gagcagatat tttgccagct tcgattgata ttattcgtga tgttatcaaa
    33061 actggtatgg tacctacaat tgattcagct aaaacacttg ggattgtatc tttgccaggg
    33121 atgatgacag ggttaattct tgctggaact tcacctatcc aagcggttaa ataccaaatg
    33181 atggtgacgt ttatgctatt agcaacgact tcgatagctt cttttgtagc tacttatttg
    33241 gcttataaaa ttttctttaa taatcgtaag caattagtag taacaaaaga ttgattcatt
    33301 ttaataaaca gtatgttagt ctggtaagta tagtccaggc ttttttggta tcttaatctt
    33361 atttttgatc gaaaattgac caagttattg agtcgattac gtttaaatgc tactatttaa
    33421 tagaaattgt tgtagggatt atcaaaagga ggaagaaatg attttaaaaa tttgtcgtgc
    33481 agcatatagt ttacaatggg gaggtgttta ccaattagct ttgctggatt atcctcgaat
    33541 taaggcgttt gaattggaaa ggataggagc tttcatagct tacgagaaac aatataaaag
    33601 aaaaactgag atacaatgtg acgataaaca tctcctcgca aaaattgttc attttttaaa
    33661 atacaatagt tttacttttc cctatattcc caaatataga gaagcggcag ctacttttaa
    33721 tgaggatggt attagtttaa cttctgattt tttaagccat acatgtacga ttgaaactgc
    33781 aaaactaatt tttaaagaag gtaaaatctt atcagcagtt aaagccttta ataagcctgc
    33841 tgaagtactg gtaaaagata agaggaatgc tgctggagac cctaaagatt actttgacta
    33901 tgtgatgttg aactggtcaa ataccaattc tggttatcgt ttagtaatgg aaagattgtt
    33961 aggcaaagca ccatctgaac aggagttaac agtaggtttt aagccagggg tcagttttca
    34021 ttttacttat caagatatca tcaatcatcc tgattctatt tttgatggtt atcatcctgc
    34081 taaaattaaa aatcagcttt ctttagcaga acatttagtt gcatgtgtta tcccaaaaca
    34141 ttatcaagaa gattatcaaa gccttgtgcc caatgacttg aaacacaggg tttattattt
    34201 agattactgt aacgaaacac tttatgagtg gaatcaaaaa gtttatgatt ttctttgtca
    34261 tttggaaaat aaataaaacc aaaaagttta aaactttttg gtttaactgc tattaattgg
    34321 tctttaaaag acttaaaaat tcttcaatca taggactaaa agaagtatca tttcggtaag
    34381 caataccaat cggataaaaa ttatttttat ccttaagtgg gacccagcat aaatcttgaa
    34441 cttggctaat gctttcaaat tcacttggta aaatagtgac tgcattgagg tcttgtaaac
    34501 tatgaataag aacttcccag tcattgtgtt taaagacgat atgggggtca aatcctaaag
    34561 cacgacattt acgaggcaac atctctccta gcatataatg ttcgttgagt gaggcaactt
    34621 tataatctct taaatcatta agttcaattt caggaagtgt tgcaagagga tgcgattttg
    34681 gcatgacaat gcttacttta tatcctttag ttgaggtttg cagtggttcg atagtgatat
    34741 cattgcgggt acttggaaag gacaataagc caatatcgat ctttcctttt gctaacaatt
    34801 cttgtaattt acgggaacca tcttgaatca aagatacttc aacatttgaa tgtgtagcca
    34861 taaaagttga aatctgtttc ataaattgaa tagcaaaaag agaagtaagc cctacacgga
    34921 caccttcctt tttttcttga tttaattgct ggatctccgt aaccatttga ttaacattag
    34981 tcatcaacaa ttgtcctttc tcatagagaa tttctcctgc ttcagttaaa gcgagttgac
    35041 gaccattttg taagaataaa atagtattta attctgcttc taatttttta atggattgtg
    35101 aaagtgtagg ttgggtcaca aaaagacttt ttgcagcgcg tgaataattt tttgcttctg
    35161 caacagcaat aaagtaagta agttgtctaa tatccataat atctgtcctt ttttcttagt
    35221 aatagtatag cacaagtcta tcaatctgac ctttatgatt atttccacaa taatattagt
    35281 aaaagctata cttatataat tttttccaat tttccttttg caaatagcta tgatatagtt
    35341 aatatagtta gtgaaatcac aaacaatcat tattagtagg aggcatctta tgtcggatgt
    35401 tgtcgaaaaa caaacaacta aatcgtttat aatgaacgta ttaaatggtt tagctttagg
    35461 gactgttatt gtcttgatac caggagcaat ccttggagaa ttaatgaaag ctttactgcc
    35521 aatgtggagc ggctttgcaa ctttaatcgc tgcaacagca gttgcaacaa gtatgatggg
    35581 gcttgttatt ggtattatgg ttggtctcaa ttttaaattt aatcccattc aatcagcatc
    35641 actaggatta gcggttatgt ttgcaggtgg agctgcaacc ttccttaaag gcgctattat
    35701 gctaaaagga acaggcgata tcattaacat gggaattact gctgccttag gtgtcctttt
    35761 gatccaattt ttatctgaca agacgaaatc attcactttg attgtcattc caacagtaac
    35821 actcttacta gttggaggag taggtcatgt gttattacct tatgtcaaaa tgattaccac
    35881 catgattgga caagggattg cttcattact tggtttacaa cctgttttga tgtcaatttt
    35941 aattgcgatg attttctgtt tcttgatcgt atctccaatc acaacagttg ggattgcttt
    36001 ggcaattagt ttgtccggaa ttggttctgg tgctgctaat ttaggtatct gtgctgctag
    36061 ttttgggctt tgtatggctg ggtggtcagt caattctaag gggacagcct tagcgcatgt
    36121 tttagggtct cctaagattt cgatggctaa tgtattagca aaaccgaaga ttatgctacc
    36181 tatgatatct tcagcagcaa tactaggaat tctcggagct ctctttaata ttcaaggaac
    36241 accagctagt gcaggttttg gtatcagtgg tttgattgga ccgattaatg ctcttaattt
    36301 agcaaaaggt ggatggtcag ttatgaatat gttattgata attataatat ttgttgctgc
    36361 accaattata ttgaatttta tttttaatta cctctttata aaagtactta aaattattga
    36421 tccaatggac tataaattag atatttaagg agatattagt gatgaaactc aaagtattta
    36481 atgttagaga agaagaagca acattagcac aagattgggc aaaccgaaat catgtggaac
    36541 tttctatgag tgaaggacct ttgacattgg aaactgttaa tgaggtagaa ggttttgatg
    36601 ggattgctaa tgcacagatt gagcctttag atgatgctat ttatcctctt ctcaaagaaa
    36661 tgggaattaa gcaaatcgct cagcgcagtg ctggtgttga tatgtataat cttgagttag
    36721 ctaagcaaca tggtattatc attagcaatg tgccaagtta ctcaccggag tctattgcag
    36781 aatttacagt gacaatcgct cttaacctta ttcgaaaagt tgagcttatt agagctaatg
    36841 ttcgtgaaca gaatttttca tggaccttgc caattcgagg acgagtccta ggtaacatga
    36901 ctgtggctat aataggaact ggtcgcattg gtctagcgac tgctaaaatt tttaaagggt
    36961 tcggatgtcg tgttattggt tatgatattt accataatcc aatggcagat ggtatccttg
    37021 agtatgtcaa ttcggtagaa gaagctgttg aagaagctga tcttgtgtcc ttacatatgc
    37081 caccaactgc tgaaaatacg catctattta atttagatat gtttaaacag tttaaaaaag
    37141 gtgctatttt aatgaatatg gcacgtggag cattggtgga aaccaaagat cttcttgagg
    37201 ctttagatca agggcttttg gagggagctg gtattgatac ttatgaattt gaggggccat
    37261 atatacctaa aaattgtcaa ggtcaagaca tttcagacaa ggatttccta agactgatta
    37321 accatccgaa agtaatatac actcctcatg cggcttatta tacagatgaa gcggtcaaaa
    37381 atctagtaga gggtgcctta aatgcttgtg tagaagtgat tgaaacggga acaactacta
    37441 cgaaagtcaa ctaaaagatg cttaggcatc tttttttgtg gggtaacaaa acatatgatg
    37501 attatgacct ttaaagcaaa aaaacatctt atgtatttat agcttgaaag tcttttttat
    37561 aataaaaaaa gatatttttt ataaaaatat tgaaaaaaac atcagatgaa tagtattatt
    37621 taatcataaa agcgtttaca aaatacaagg agggttctct catggagaaa acaatggata
    37681 tggaacaacg tgaagaagtt gtatcttata accgagcgaa agtatggcag ttagttatgt
    37741 ttgccatgaa caatgcttct acaaatatat acttatttac gtttatgttt gttacctatt
    37801 tctcaactgg cgttcttggc tcggcagcca tttttgtcag tcaaattatg ggttatatcc
    37861 gtatttttga tggttttatt gatccagcta ttggtattat gattgataaa actgatacaa
    37921 aatttggtaa gtatcgtcct attttgatta ttgggaacgt tattacggct ttgtcattga
    37981 tttttcttct tgcattgaga ggtgtcgatg aaaatattcg tttcccatta tttatccttg
    38041 ttctaattat ccataaaatt ggatactcaa tgcaacagac aataactaaa gcagggcaaa
    38101 cagcactgac aaatgaccct aagcaacgtc ctatttttaa tatcgttgat gcagttatga
    38161 caacatcatt aatgacaggc ggtcagtttg tcgtttcagt tttcttagta ccaaaatttg
    38221 gtaattttac acctcaattc ttcaatgtcc ttatttttgg aacaattctt atttcagcta
    38281 ttttagcaat cgttgcaatt attgggattt gggcaaaaga cagaaaagaa ttctttggtt
    38341 taggagagaa tacccaaaag actgctctaa aagattactg gaaggtgttg aaaggaaata
    38401 aaccgcttca gatattatcc attgctgcag ctttggtaaa atttgccatc caattctttg
    38461 gcgattccgt tgttatggtg ctattgtttg gtattttatt tggtaattat gctttatcag
    38521 gacaattctc acttcttttc attgttcctg gtgttattat taatattttg ttctcaacaa
    38581 ttgctcgtaa aaaaggtctt cgtttttcat atgtcagagc aattcagatc ggtatgattg
    38641 gtttacttgc ttttggtgca gttctttacg ttggtaagcc tggtgacctt agtttaacaa
    38701 gcctaaatct ttatactatt ttatttatcg ttactaatat tattgcgaga tacgcttcac
    38761 aagcacctgc tagtttagta ttgactatgg gagcagatat ttctgactat gaaacttctg
    38821 aatccggtcg ttatgtatca ggaatgattg gaactatctt ctccttaaca gattcgattg
    38881 cttcttcatt tgctcctatg gtagtaggtt ttgtgttagc aggaattgga ttttcaaaat
    38941 catttccaac tatcgaaaca ccactgcccc ctgatttaaa aatggcagct attagtatcc
    39001 ttgtggccat tccatttata gctttgtcta tagctcttct tttaatgaaa ttttacaaac
    39061 tagataaaga agaaatggtt cgtattcaag agaagattca agttatgaaa gcagcaacta
    39121 ctaaggaacg tgctaaagat attgctcgta atgttccttt gacagatatg gattatgttg
    39181 atgtaacaaa atatcctatt gataaagatt aaaaagttta gctgctcttc aatagagcag
    39241 ctattttgtt aaataagagc taccattgga ggattcttat ggcaaaaatt atttcattag
    39301 gtgaggttct tttgcgatta tctccacctc aatatcacac actgatgcag gctaatcacc
    39361 taaaatgcca attcggtggt tcagaactta atgtcttagc aagccttgct caactaggtt
    39421 accatgtagg attggtctca gctttgcctg ataatgattt gggcaagatg gcaagtcaat
    39481 ttatattgtc acagcaaatt agtccagctg ctataattaa aaaagaagga cgactaggaa
    39541 tttattacta tgaacaagga ttttcggtaa gaacaaataa ggttatctac gatcgtaact
    39601 attcatcttt ttgggaaagt accttgagtg attatgattt tacttctatt tttaagggag
    39661 tggattggtt tcatgtttct ggaattactc cagcactaac caaggactta tatgaggtga
    39721 ctcggttcct tatgacaaaa gctaaagaag gaggtgtaaa agtatcgatt gatctcaatt
    39781 ttcgtgagag tttatggtca tcttttcaag aggcacgtga gcaattatcg ccactattag
    39841 gattattaga tgtttgcttt ggcttagagc cgatttattt agctggcgaa agtgaagatt
    39901 taaaagatga gttgggctta tccagacctt accttgatat tgaattactt gaaaagataa
    39961 cccaaaagat tgtccaagaa tatgggttag actatattgc ctttactcaa agggaaatgg
    40021 aatatactaa tcagtatatg ttaaaatcat atttgtatca taataatatg ttatatcaaa
    40081 ctgataaaac tggcgttgaa gtattagatc gtgtggggac aggagatgcc tttgctgcag
    40141 gtttaattca tgcgcttctg gaaaaagaaa ctcctcaaag ggccttagaa atagctatgg
    40201 ctacatttaa atacaaacat accattcaag gagatatcaa tatcatgaca cgagatgata
    40261 ttgcttacct cattgaaaaa gaaacaaatg atattaaacg atagtgtaaa aggagagtgt
    40321 atgttatatc cattattgac aaaaacaaga aatacttatg atttaggcgg tatttggaac
    40381 tttaaattag gagaacataa tccaaatgaa ttactacctt cagatgaagt catggttatc
    40441 ccgacttcat ttaatgattt gatggtaagt aaagaaaaac gtgattatat aggtgatttt
    40501 tggtatgaaa aagtcattga agtacctaaa gtatcagagg atgaagagat ggtgctgcgt
    40561 tttggctcag tgacacatca agctaaaatt tatgttgacg gtgtattggt aggagagcat
    40621 aagggaggtt ttactccttt tgaagttctt gttcctgaat gtaagtataa taatgagaaa
    40681 attaaggtat caatttgtgc taataacgtt ttagactata ccacgcttcc ggtcggaaat
    40741 tatagtgaaa tcattcaaga agatggtagc attaagaaga aagtacgtga gaattttgat
    40801 ttcttcaatt atgcaggtgt gcatcgtcct cttaagttga tgatacgtcc taaaaatcat
    40861 atttttgata ttacaattac aagtcgtcta tcagatgatt tacagtcagc agatttacat
    40921 tttttagtgg agactaatca aaaggttgac gaagttagaa ttagcgtttt tgatgaagat
    40981 aacaaactag tgggtgaaac gaaggatagc agattatttc ttagtgatgt ccatctttgg
    41041 gaagttttaa atgcctatct gtacacagcg cgtgttgaaa tttttgttga taatcaactc
    41101 caagatgtct atgaggaaaa ttttggtctt agagaaatag aagtgacaaa tggtcaattc
    41161 ctattgaatc gcaaacctat ttattttaaa ggatttggta aacatgaaga tacgttcatt
    41221 aatggcagag gtttgaatga agctgctaac ttaatggatt tgaatctttt gaaggatatg
    41281 ggggcaaact cttttagaac atcccattat ccttattctg aagaaatgat gcgtttagca
    41341 gatcgaatgg gagtgttagt gatagatgag gttccagcag tagggttatt tcaaaatttt
    41401 aacgcttcct tagatttgtc accaaaagat aatggtacgt ggaatttgat gcaaacaaaa
    41461 gcggcgcatg aacaagctat ccaagaatta gtgaagcgtg ataaaaatca tcctagcgtc
    41521 gtgatgtggg tagtcgctaa cgaaccggct agtcatgaag cgggagcaca tgattatttt
    41581 gagccattag taaaacttta taaagattta gaccctcaaa aacgtcctgt caccttggtt
    41641 aatattttaa tggcaacccc agatagagac caagtgatgg acctggttga tgttgtctgc
    41701 cttaatcgtt actatggttg gtacgttgac cacggtgatt taacaaatgc agaagtaggt
    41761 ataagaaaag agttattaga atggcaagat aaatttcctg acaaaccaat tatcataacg
    41821 gagtatggcg ctgatacgtt accgggatta cattctactt ggaatattcc atatacagaa
    41881 gaatttcaat gtgattttta tgaaatgagt catcgtgttt ttgatggtat tcctaattta
    41941 gttggtgagc aagtctggaa ttttgcagac tttgaaacta atctgatgat acttcgtgta
    42001 caggggaatc acaaaggtct cttttcaagg aatcgccagc cgaaacaagt cgtcaaagaa
    42061 tttaaaaaac gctggatgac tattcctcat taccataata aaaaaaatag tgtaaaatag
    42121 gggtagttat tttagaagag gtgatgatat ggctagaccc ttagtagaac aaacagcaga
    42181 tcgtctattg catttaattt tagaaagaga ataccctgtt ggtgctaagc tccccaatga
    42241 atatgaatta gcagaagatc tagatgttgg tcgcagcaca atccgagaag cagtcagaag
    42301 tttagcaacg cgtaatattt tagaagtcag acagggctct ggaacatata ttagttctaa
    42361 aaagggtgtt tctgaggatc cattagggtt ctctctaata aaggacacag ataggttgac
    42421 atcagatctc tttgaactac gccttttatt ggaacctcga atagcagagc tggtagctta
    42481 tagaattaca gatgatcagt tacaattact cgaaaagctt gttggagata ttgaagatgc
    42541 tgttcacgca ggcgatccga aacatttact tttagacgtg gaatttcatt ccatgctagc
    42601 taaatacagt ggtaatattg caatggatag ccttttgcca gtcatcaacc agtcaattca
    42661 tctgataaat gcgaattata caaatcgtca gatgaagtcg gatagtttag aagcacatag
    42721 agaaattatc aaagcaatcc gagaaaaaaa tccagtagct gcacatgatg ctatgcttat
    42781 gcatatcatg agtgttagac gttcagcgtt gaaagcttaa tataagaaga aaaatagcta
    42841 ttcttgaata gctatttttt tagtattctt cttgacaatt catcagatga cttatataat
    42901 atagttaaag ataacggtta cattttggga ggtattatgt taaatcaatt gaaaaacaac
    42961 tatttctttg ctgttattcg agggaaatca tctgaggatg cgctcgaaat cgcaaagcat
    43021 gctattttgg gtggtattcg gaatattgaa gtaacttttt cgactcctga agcaagcaaa
    43081 gtcatcaaac aattatcaga tgactttaaa aataataaag aaattattgt gggggccgga
    43141 acggtcatga caactgaact tgctaaagag gctattgatg ctggagctaa atttttggtt
    43201 agcccacact ttgattcaga tattgctaat ttagctaatg aaaacaaggt ttattatttc
    43261 ccaggctgtg caacggcaac agaaatcgtg gtagctagaa agtataaatg tcagattata
    43321 aagctttttc caggtggagt tgtgggtcca ggttttatca aagatattca tggtcctatt
    43381 ccagatgttg atttaatgcc atcaggtggc gtctctgttt caaatgtagt tgaatggaga
    43441 aaagcaggag cagttgcggt tggtgttggc tcagcattat ctagtaaagt agccacagaa
    43501 ggctatgata gtgttactaa aatagctaaa cagtttgttt ctgcacttga ttagtgagta
    43561 agagaagaat atggcgttta atactgaaac atttatgtta aaaaatcaag cagctataca
    43621 gctgtatgaa gaggtcaaga gacaacctat ttttgattat cattgccact tagatccaaa
    43681 ggacattttt gaagatcata tttttgataa tattgtggat ttgtggcttg gtggtgacca
    43741 ctataaatgg cgtcttatga gggctaatgg tatttctgaa gcagaaatta caggtccagc
    43801 atccaacctt gagaaattta aagcatttgc tagaacacta gaaagagcat acggtaatcc
    43861 tgtttatcat tggtctgcta tggaattgaa aaatatcttt ggtgttaatg agattttgac
    43921 agaatcaaat gctgaggaaa tttatcatcg tttaaatcat ttcttgaaag agcataaaat
    43981 aagtcctaga cgtttaattg cagatagcaa agttatgttt attggtacta cagaccaccc
    44041 tcttgatact ctcgagtggc ataaaaagtt ggctgctgat gagagtttta aaacagttgt
    44101 tgctccgaca tttaggccag atgaagcttt tattgaacat cgacattttg ttgattttat
    44161 tactaaactg ggtgacatta ctcaaaaaga gattacagat ttttcaacat ttatagcagc
    44221 aatggaagaa cgcattgcgt attttgctca aaatggttgt agggctagcg atatcagttt
    44281 tacggaaatc gtctttgaac aaactgatga attggagttg aacgatttat ttaataaagt
    44341 ttgtgaaggc tatatcccaa atcaatcaga aatcagcaaa tggcaaacag ctgtttttat
    44401 ggaactttgc cgattatata aaaaatatgg ttttgtaaca caggtgcatt ttggagcttt
    44461 gagaaataat cattctacta tttttgaaaa attgggagct gacgtgggtg tggattcact
    44521 tggtgaccaa gtggctttaa ctgttaatat gaaccgttta ttggattcct tggttaaaaa
    44581 agatagtctg cctaaaatga tttggtataa tctcaatcca gcttacaata tagcagtagc
    44641 taacactttg gcaaattttc aagctaacga acttggagtg agatcttacc ttcaatttgg
    44701 tgcaggttgg tggtttgcag atactaaatt agggatgatt agtcaaatga atgccttagc
    44761 agagcaaggc atgttggcga attttattgg tatgttaaca gattctagaa gttttctatc
    44821 ttatcaacgt catgactatt ttcgacgtat tttatgtacc tacttaggag aatggattga
    44881 agaaggagaa gttccagaag attatcaagc tttaggaagt atggctaaag acatcgctta
    44941 tcaaaatgca gttaattatt ttaagaacta aaaaggaaga gtgaaatcat ggaaatgtca
    45001 tttaggtggt atggtgaaga cgaccccgtt actttagaaa atattggtca aattccaaca
    45061 atgaaaggga ttgttacagc gatatacgat gtaccagtag gagaagtttg gtcacgtgag
    45121 cgcattcaac agctgaaaga aaaggtagag gcagcagggc ttaaaatttc ggttatcgaa
    45181 tcagtacctg ttcatgaaga tatcaaattg ggacgtccaa cacgtgatct cttgattgat
    45241 aattatattc aaaccgtcaa aaatttggct gcggaaggta ttgatactat ttgttataat
    45301 tttatgccag tatttgactg gacacgcact gatttagcat atcaatatcc agatggttcc
    45361 acagctttga tatttgatga gacggtttct aaaaaaatgg atccagtaaa cggtgaatta
    45421 tctttgccag gatgggatgc cagctatagt aaagaagaaa tgaaggctat catggatgcc
    45481 tatgctgaaa ttgatgagga aaaactttgg gaaaatctca cttattttat taaacgtatt
    45541 attcctgaag cggaagccgt aggcgttaaa atggccattc atccggatga cccaccatat
    45601 tctatttttg gtttacctcg cattattact gggttagagg ctattgagcg ttttgtgaaa
    45661 ttgtacgata gtaaaagtaa tggcatcact ttatgtgtgg gttcttacgc ttctgatccc
    45721 cagaatgatg ttttagagat ttcgcgacgt gcttttgagt tagatcgtgt aaactttgtt
    45781 catgctcgca atattaaatt aggtgatgga aagtcattta aagaatcggc tcatccatca
    45841 gaatacgggt caatagacat gtatgaagtt attaagttat gccatgaatt tgggtttgag
    45901 ggtgctattc gacctgacca cgggcgtatg atttggggag aaacaggtcg tccaggatat
    45961 ggcctatacg atagagcttt aggagccact tacgtttctg gtttatacga agctgtgatt
    46021 aaaggttcaa aataaggcga tgcttccaaa tttctcctgt tccaatctcc tgaaagagaa
    46081 atttggaaag ttattatcat aatatgattg tacttagcta agagtagaat acttatagaa
    46141 gttaatgaga ggaataagat gtcaaaaaca attacattta ccaataaagt tgtcgtcata
    46201 acaggtgcag gcggtgttct ctgtggttat atggctaaag aatttgcgaa agcaggcgct
    46261 aaagtggcat tattagattt gaatcaagaa gctgctcaaa catttgctga tgaaattgta
    46321 gaagaaggtg gtatcgctaa ggcttataaa gctaacgttt tgtctaaaga aaaccttgaa
    46381 gaagtgcatc aagccgtttt agaggatctt ggaccaactg atattttagt taatggtgca
    46441 ggaggcaaca atcctaaagc tacaactgat aatgaatttc acgaattaga cttaccttca
    46501 gaaacaaaaa ccttctttga gttagatgaa gctggtatta gctttgtttt taacttaaat
    46561 tatttaggaa cattattgcc aacgcaagta tttgcccaag atatggttgg acgtgaagga
    46621 gcgaatatta ttaatatctc ttcaatgaat gctttcaccc ctctgaccaa aataccagct
    46681 tattctggag ctaaagcagc tattagtaac ttcacacaat ggctagctgt tcatttctca
    46741 aaagttggta tccgttgtaa tgctattgca cctggtttct tggtaacaaa tcagaaccga
    46801 agcttgttat ttactgagga cggtcagcca actgcgcgtg ctgagaaaat tcttaataat
    46861 acaccaatgg gaagatttgg agaagcaagt gagctgattg gcggattgtt ctttttagca
    46921 gatgaaaaat catcaagttt tgttaatggc gttgtccttc cgattgatgg tggatttgct
    46981 gcttactctg gtgtttaatt tacaagataa tagccactta acacataaga gattattgag
    47041 ttgagtggtt ttttaaggaa atttgtttaa tgatcttacc actaaatgat gggtaggttg
    47101 ctaattggaa aatcctcata agttagataa atttagaaac tggagataat atggtagata
    47161 caaatcacaa aatcgctttt tgcatagatt ctgatggttg cgcaatggat acaatgacat
    47221 ataagcatca actctttttt ggtccaattg ctgcagatat atttgggata tcagaacaag
    47281 aaagaacaag ttttttaaaa gagtgggata gagtcaatct ttattcaaga actcgtggag
    47341 ttaatcggtt tgtgggattg gttatgggcc ttgagtttgc cggagtagaa catattgata
    47401 aattaaaaga atgggtcgaa acaacagact cattgtccaa taattcctta gagttagcgc
    47461 ttgccaacga ttttagtaga gatttagaga aggctttaga atggtccaat gaagtgaatc
    47521 gccaaatcaa aaattatgag ggagattctt tagcgttttc aggtgtgtta gaaagtttag
    47581 aacgattaag tcaatttggt aatgtttatg tcgtgagttc tgctaacaag gaagctgttt
    47641 ggcaagagtg ggaagaacag ggattgctaa agtatgttga tgatatctat tgtcaagata
    47701 gaggtaaaaa agaagatgtt atcgccactt taattgcaag tggttactgc cgtgaaacta
    47761 ttttgatggt aggagattcc ctgggtgatc tggctgcagc agaacaaaat ggtattgcat
    47821 tttacccagt acttgttggt aaggaagtca aatcatggga aattcttaga gaagatattg
    47881 gtgaagcttt tgcaaaaggt caattcgaac agcaaaggca aaaagaatct attaatacct
    47941 tttgggctaa tttagataac taatagaaag gataggctat gacacattta gttgacttaa
    48001 caaagaaacc cttcaatcta aatcaagaag caattgagtg gattgaaaaa acaattaatg
    48061 agatgacatt ggatgagaaa attgggcaac tttttttcaa tatgggagct agtcgttcag
    48121 aagaatattt aacagatgtt ttggatcggt atcatattgc tgctgttcgc tataatagag
    48181 gtagttcaag tgaaatctat gatcagaatt taattttaca gacaaagtcg aaattgccga
    48241 tgttgattgc tgcaaataca gaagctggtg gagacggtgc tgtcacggat ggaactaaag
    48301 ttggcgatga aataaaagtg gctgctacaa atgatccgaa atatgcttat gaaatgggaa
    48361 gaatcgcagg gatggaggca tctgctgtgg gttgtaatgc ttcattttct ccaattgtag
    48421 atttgacacg taattggcgt aatcctatca tagctagtcg aaattggggt gctaacgttg
    48481 atcaaattat ttcactttca aaagagtata tgaaaggaat catgcaatat aatattgtac
    48541 ctttcgctaa acattttcca ggggatggca ttgatgagcg tgatcatcat ttgtcatttg
    48601 cttccaatcc tatgagtaaa gaggagtgga tgtcgacatt tggccgcatt tatggtgaat
    48661 tggctgacgc aggtttacca ggagtcatgg caggtcacat acatttacca aacgttgaaa
    48721 aagaaatgca tccagagcga gaccttgatg atatgttacc agcttcattg aataaaacac
    48781 ttttagatga gttattgaga ggagagttag gttataatgg agctattgta acggacgctt
    48841 ctcatatggt tggaatgact gcttcaatgc caaggagaga tttgttgcca acggcgattg
    48901 aagcaggttg tgatttattc ttattcttta atgaccctga tgaagacatt caatggatga
    48961 aagaaggtta cgaaaaaggt attctaacag aggaacgtct ccatgacgct ttacgtcgta
    49021 ctctcggtct aaaagcgaaa ttaggcttgc ataactatga gggacgtcgc caagaactct
    49081 ttatgcctaa agataaggct atggctttaa tcaacacatt agagtcgcaa aaaatagctg
    49141 atgaagttgc tgataaagca gtaactctgg taaaagataa acaaaaagat atctttccag
    49201 taaatccaga acgttatcgt catatcttac ttgttaacgt tgaaggatat aagggaggtt
    49261 ttggtgcgat gattgctgga aacaagcaac gtgcatcgga ttacatgaaa gagttacttg
    49321 aggcacgtgg ccatgaagta actgtctggg agtctaccga agagcgtatt atgaaattgc
    49381 cacaagagga gcgggcagca gctatcgcta atgtttacgc acaaaaacag ccaatcgcaa
    49441 atctaacaga acactatgac ttgattatta acttggtaga tgtcaatgct ggaggaacaa
    49501 cacaacgtat tatttggcca gcagctaaag gcacccctga tcagcctttt tacgttcatg
    49561 aaataccatc tattgtgatt tcagtacaac atgcatttgc tttagcagat atgccacagg
    49621 tagggactta tattaatgct tatgatgggc ttcctagcac gataagtgca gtagttgcga
    49681 aattggctgg agaatcagaa tttacaggag tttcaccagt agatgcttac tgtggtttaa
    49741 ttgatacaca tttatggcgc caatgctaat agaaacaaga tgatgtactt ttatgtaaca
    49801 taaaaaaaga gcttaaagct ctttttttat ccttcaaaat agagtagttc cttgggtgtt
    49861 ttggtaaata cttcaaagcc ggttttagtc acataaccgc agtcttcaat tcgcacacca
    49921 actttatctg gtatataaat acctggttca acagagaagc acatgccttc ttgaatctcc
    49981 atatcgttac cagccataat agatggaaat tcatggacat ccatacctaa tccatgacca
    50041 aggcggtggt taaagtattg tccataacca gccttttcaa taacatttct tgctgctgcg
    50101 tcaacttcag aagcaaggac tcctggctta ataaaatcaa tagccgcttg atgtgcttca
    50161 agacataaat ggtaaatatc ttttttgaat tgatcaggtt tccccactgc tactgtacgg
    50221 gtcatatctg acgtataacc tagtgtttct acacctaaat caaataataa caaagcatta
    50281 ttttcgattt tattagttcc tgggatacca tgtggatttg ctgcattatt gccagtcaaa
    50341 accatagtgt cgaaactcat tttattgata ccttgttttt tcatttcaaa ttcgatttga
    50401 gcgataatat cggtttcagt gttgtttagc gagatattgt caaatccaac ttgaacagct
    50461 ttatcagcaa attctccagc gactaacatt ttctcaattt catctcgtga tttaattagg
    50521 cgcatgtttt gaatataagg tgttaggttt tcaaaatgac catcaaaaac ggtttggaga
    50581 ccttggaatt tagtaacatt aagattatca aattcggcta gaactttaga aacagaaaag
    50641 gatggtaagt tgctagctat tttttgccaa ggattttctg aatctatgta accaaagacg
    50701 ggaaatggta cactttgttt tgcacgcgat acttctagtg ctggtacaaa taaaatagga
    50761 tcgcgatctt catagacaaa taaaaacatt tgtctttcat ggggatcgca aaagaaacct
    50821 gttaaatagt tgactgtcac aggatcagaa aagacagcaa gttcagcttg gactgaatgt
    50881 aagtgatggc gtatacgatt aagttttgac ataataaccc ttcttcctaa aaggaactta
    50941 gtctagttag gctagtttct tatgcctatt atttttccaa aaaaatagga aaaaatcaag
    51001 tttagttagt attttgaaag tttttcataa aaaagcttta taaaagcttg aaagtgtttc
    51061 caaaaggtga taaaatatca gtgaaagcgt cattttcaaa tagaaaggaa gttaaatcaa
    51121 tatgaataca gatgatacga ttacgattta tgatgttgcc cgtgaagcag gtgtttcgat
    51181 ggcaactgtt agtcgtgtgg taaatggaaa taaaaatgtc aaggaaaata cacgtaagaa
    51241 agtccttgaa gttattgatc gtcttgacta tcgtccaaat gctgtggcgc gtggtttagc
    51301 aagtaaaaaa acgacaactg ttggtgttgt cattcctaat atcgcaaata gctacttttc
    51361 aattttagcg cgtggtattg atgatattgc agcaatgtac aaatataaca ttgtccttgc
    51421 ttcaagtgat gaagacgatg ataaagaggt taatgttgtt aatactttgt ttgctaagca
    51481 agtagacggt attattttta tgggacacca cttaactgaa aagattcgtg cagagttctc
    51541 tcgttcacgc acaccgattg ttttagcagg tactgtggac cttgagcatc aattaccaag
    51601 tgttaatatc gattacaaag cagcagcagt tgatgttatt gatattttag ctggcaatca
    51661 taaagacatt gcttttgtat caggaccact tattgacgat attaatggca aggttcgttt
    51721 ggcaggatac aaagaaggtc tcaagaaaaa tggcttaaac tttaaagagg ggttggtttt
    51781 tgaagccaac taccgttatg ctgaaggctt tgcattagct caacgtgtga ttaatgctgg
    51841 agcaacagca gcatatgttg ctgaggatga gttagcggca ggtttgttaa atggtctttt
    51901 tgaggcaggt aaacgtgttc ctgaagattt tgaaattatc acaagcaatg actcgccaat
    51961 cgcacaatat acacgtccta acttaacttc tatcagtcaa ccggtctatg atttgggtgc
    52021 tgttagcatg cgtatgctaa caaaaattat gcacaaagaa gagttggaag aaaaagaaat
    52081 agtgcttaat cacgggattg ttaaacgtgg cacaacaaat aattagtgtt agagtatgtt
    52141 atttgaaaaa tagtggtaga atggtagtag cttagcgctt aactttctga tacttaacat
    52201 gatatattga gaggattagg gtagcttagc ttcctaatct ttttttagag gtgtaatatg
    52261 acaaatgaat tgataatgca agcttttgag tggtatttac ctagtgatgg gaatcactgg
    52321 aagaaattag aggagtctat atcagacctt aaaaaacttg gaattagtaa aatctggtta
    52381 ccaccagcat ttaagggaac tagcagtgat gatgtaggat atggtgttta tgatctcttt
    52441 gatttaggag aatttgacca gaatggaaca attagaacaa aatatggtag gaaagaagag
    52501 tatctaaagc ttattaagtc gttaaaggca aatggcatta aaccgtttgc agatatcgtt
    52561 cttaaccata aagccaatgg tgatcataaa gaaaaatttc aagtcatcaa agtcaatcct
    52621 gaaaatcgtc aagaagcatt aagtgaaccc tatgagattg aaggatggac gggatttgat
    52681 ttcccaggta gacagggtga gtacaatgat tttaaatggc actggtacca ctttactggt
    52741 ttagattacg atgctaaaaa taatgaaaca gacatcttta tgattgtagg cgataataaa
    52801 ggttgggcag acgatgattt gattgatgat gaaaatggta atttcgatta tctcatgtac
    52861 aatgacattg attttaagca tcctgaagtt ataaaaaact tacaagattg ggctaagtgg
    52921 tttattgaaa ctacaggcat tgaaggtttt cgtttggatg ctgtgaaaca tatcgattct
    52981 tactttattc aaactttcat taatgatatt cgtacaaaaa taaaacccga tttagaggtc
    53041 tttggcgaat attggaaatc tgaccaaaca tcaatgaagg actatttaga ggcaacacaa
    53101 tttcaatttt ctcttgttga tgtcaccttg catatgaatt tttttgatgc cagtcaccaa
    53161 aaccgtgatt ttgatatgcg aacaattttt gatgatagtc ttgttattga taatcctgag
    53221 tatgctgtta cattcgtaga aaatcacgat acgcaaagtg gacaagctct agaaagtaga
    53281 gtggaagatt ggtttaaacc tttagcttat ggcttgatac ttttacgcca acaagggact
    53341 ccatgtcttt tttatggtga ttactatggc attcaaggag aatttggcca accatccttt
    53401 aaagaggtta ttgataaaat ggctgagcta cgccaaaact atgtatttgg cgaacaagtc
    53461 gattacttta cgcattcaaa ttgtattggt tggacttgtc ttggggatga ggaacataat
    53521 tcttgtttag ctgtggtcct aacaaatggt gatcagggat ggaaacatat ggaagtaggg
    53581 gaaatttatg caggcaaaac ttttgttgat tatttaggca attgtgaaca agaggttgtt
    53641 ataggagatg atggttgggg tgatttttta gttgcatctg cgtctatctc ggcttgggtc
    53701 cctaaaatag aggaagctaa ctgataaatc aggtgaaaaa aaaggtaaaa tgagatataa
    53761 taagacctat gaaagtttta ctgtatttag aggctgaaga atacttgaaa aagtcaggta
    53821 tcggtcgtgc tatcaaacat caagagaaag ctcttcaaat tgctgggata gattatacca
    53881 ctaatcctac agatgacttt gaccttgttc atatgaatac ctatggtatt agaagttggc
    53941 ttttgatgag taaagctaaa aaaactggga aaaaagtaat tatgcatggc cattccactg
    54001 aagaggactt tagaaattct tttattggct caaatttagt atccccgctt tttaaatggt
    54061 acctttgccg tttttatcaa aaagcagatg ccattataac cccaactgat tattctaaac
    54121 aactaataaa agcatatggc atcaagaaac caatttttgt tttatctaac gggattgatc
    54181 taagtcgcta tcagaggtca gaaaagaaag aagctgcatt tcgtcactat tttcacttaa
    54241 gtaaggatga taaagttgtt atgggggccg gtctttattt catgcgtaag ggaattgacc
    54301 aatttgttga agtagctgca aaaatgcctg atattcgttt tatttggttt ggagaaacaa
    54361 ataaatgggt tattcctcga aaggttaggc aaatcgttac caagcaacat ccctcaaatg
    54421 ttacttttgc tggttacata aaaggtgatg tttatgaagg tgccatgagt gctagcgatg
    54481 ctttcttctt tccgagtcgt gaagagacag aagggatagt agttttagag gctttagcta
    54541 gtcatcagca tgtggtttta agagatatcc ctgtgtatca cggttgggtg accgaagaca
    54601 gtgttgaatt agcgactgat gttgatggtt ttgttgaaaa gttggataaa gtcttatctg
    54661 gtaagagtga taagattaaa gaaggttatc gtgtggctga gagtcgaagt atcgaacgaa
    54721 ttgcccatga attggcaagt gtttatcaaa aagtaatgga gttgtaagat gcgtataggt
    54781 ctatttacag atacatactt tccccaagtt tcgggagttt caacaagtat ccgaacttta
    54841 aaggaagggt tggagaagga aggacacgaa gtttatattt tcacaactac cgaccgtaat
    54901 gtcaaacgtt ttgaagaccc tacaattatt cgtttaccaa gtgtcccttt tatttctttt
    54961 acagatcgtc gagtagttta tagaggttta atttctgctt ataggatagc taaagactac
    55021 gaacttgata ttattcatac tcaaacagaa ttcagtttag gacttttagg aaaattagtt
    55081 gcaaaagctt taagaatacc tgttgtgcat acttatcata ctcaatatga ggattacgtt
    55141 ggttatatcg caaaaggcaa attgattaag ccaagtatgg tcaaatatat catgcgtacc
    55201 tacctaagtg atttggatgg tgtcatatgt ccttctcgaa ttgttctcaa tcttctagat
    55261 ggttacggtg tcaaaattcc aaaacaagtt attccgacag gtattcctgt tgagaactat
    55321 cgtcgagagg atatctctga agaaacaatc aagaatctac gaacggagct tggccttgcg
    55381 gataatgata caatgctatt gagtttatca cgggtatctt ttgaaaaaaa tattcaagca
    55441 atcttgatgc atttatcagc tgtcgttgat gaaaatcctc acgtaaaatt agtgatagtt
    55501 ggagatggtc cttatctttc agatctaaaa gaacttgttc actctttaga acttgaaaat
    55561 tcagttattt ttacaggtat ggttgagcat agccaagtgg ctatctacta taaagcatgt
    55621 gatttcttta ttagtgcttc aacaagtgaa acgcaaggat taacttacat tgagagtctg
    55681 gcgagtggac gtccaatcat tgcgcaaagc aatccttatc tagatgatgt tatttcagat
    55741 aaaatgtttg gaactctgta caagaaggaa agtgatttgg cagatgctat cctagatgca
    55801 atagcagaga cacccaaaat gactcaagaa gcttatgaac agaaattata tgagatttct
    55861 gctgagaatt tcagcaagtc tgtatatgct ttttacctag actttctgat ttcacaaaaa
    55921 gctagtgtga aagaaaaagt ctctctgacg ataggaaata aggatagcca ttctactctt
    55981 cgttttgtca gaaaagcggt atatttacct aagaaagttt ttacctttac aggaagagcc
    56041 tctaaaaaag tggtaaaagc acctaaacgt cgtatttcat ctattcgaga ttttttagat
    56101 tgagacgaat aaaattagta aatgtcttga aattataaaa aatattgtta taataaaaaa
    56161 tgaagacatg ttaggttcta atagatttca agagcgagca ctggttggtg gaaagagtgc
    56221 aattagagaa cttagtacta cttccttgat tttatgtaaa cataaaaggg tggttccctt
    56281 atcgccacta gaggtctgac acaagtatac tcgagttttt gtgttggata aattgaggtg
    56341 gaaccacgta gtctttacgt ccttttggat gtaaaggctc tttttttata aaagaagtgg
    56401 ttgcataaaa gaggcagatt gcttttctaa tgttatactt attatcccta tctaggataa
    56461 tgtggaattc aatcaaaggt caatcccttt gtttattagt gaattaatgt gtttaggaat
    56521 tggtatcacc ctaacagtta ttaagaaaag gagaacaaga tgattaaaat cactttccca
    56581 gatggtgcaa ttcgtgaatt tgaatctggc atcacaacat ttgaaattgc acaatcaatt
    56641 tctaactctt tagctaaaaa agctttagct ggtaaattta atggtcaact tattgataca
    56701 acacgtgcta tcgaagaaga tggtagtatt gagattgtaa cacctgacca cgaagatgct
    56761 ttaggagttc ttcgtcactc agcagcgcat ttatttgctc aagctgcaaa acgccttttt
    56821 ccagacttat gtttaggtgt aggtccagct attcacgatg gtttttacta cgatactgat
    56881 aataaatcag gtcaaatttc aaatgatgat cttcctcgta ttgaagaaga aatgaaaaaa
    56941 attgttaagg aaaatcaccc ttgtattcgt gaagaaatca gtaaagaaga agcgttagaa
    57001 ctttttaaag atgaccctta taaagttgaa ttaattagtg aacatgctga agatggttta
    57061 actgtttatc gtcaaggtga atttgtagac ctttgtcgtg gacctcatgt cccatcaaca
    57121 ggacgtattc aagtctttca tcttctaaat gtcgctgggg cttactggcg tggtaacagt
    57181 gataatgcta tgatgcagcg tgtctatggt actgcttggt ttgataaaaa agatttgaaa
    57241 gcatatctta aacgccgtga agaagctaag gaacgtgacc accgcaaatt aggtaaagaa
    57301 cttgatttat ttatggttaa tcctgaagta ggtcaaggat tgccattctg gttgccaaat
    57361 ggagcaacta ttcgccgcga attggaacgt tacattgttg ataaggagat tgcttcaggt
    57421 tatcaacacg tttacacacc tccgatggca tctgttgaat tttacaagac ttctgggcac
    57481 tgggaccact atcgtgaaga tatgttccca acaatggata tgggagatgg ggaggaattc
    57541 gttcttcgtc caatgaactg cccacaccat attgaagttt ataaacatca tgttcactca
    57601 tatcgtgaat tgccaattcg tatcgctgaa cttggtatga tgcaccgtta tgaaaaatca
    57661 ggggccctca caggattgca acgtgtacgc gaaatgacac tcaacgatgc ccatattttc
    57721 gttacacctg agcaaattaa agatgaattt cttaaagctc ttaatctcat agcagaaatt
    57781 tatgaagatt ttaacttaac agattaccgt ttccgcttat cttatcgtga cccagaagat
    57841 aaacataagt attatgataa cgatgagatg tgggaaaatg cacaagctat gctaaaagag
    57901 gctatggatg attttggatt agattatttt gaagcagaag gcgaagcagc cttttacggt
    57961 cctaaattgg atatccaggt gaaaactgct cttggtaatg aagaaacatt atctactatc
    58021 caactagatt tccttcttcc agaacgcttt gatcttaaat acatcggtgc agatggtgaa
    58081 gaacatcgtc caattatgat tcaccgcggt ggtatttcaa caatggagcg atttacagct
    58141 attttaattg aaacttacaa aggtgccttt ccaacttggc ttgctccaca acaagtttct
    58201 gttattccaa tttcaaatga agcccacatt gattatgctt gggaagtggc tcgtgccctt
    58261 aaagatcgtg gtattcgtgc tgaagtggat gaccgtaacg aaaagatgca atataagatt
    58321 cgtgcggcgc aaacccaaaa aattccatat caattgattg ttggtgataa ggaaatggaa
    58381 gaaaaagccg taaatgtgcg tcgctatgga agtaaagcga ctgaaacaaa atctatagag
    58441 gaatttgtag aaagtatttt agctgatatt gcaagaaaat ctagacctga cgaagttaaa
    58501 taaattttat tagtcattat aaaaatcatt aataaatgat gtgaactgat tactgtatga
    58561 tgcagaatca gttttttaga tgctaatttt ttcttgatgt ttaattataa gctaattata
    58621 gataaataat ctcatataac attcacaaaa ttagattttt tggtataatt aatatgattg
    58681 acacataaag gaaaatagta ttaataaaaa ggggtatgga gcattgcgaa cagtattagt
    58741 agtgcaaggt gatgacgaaa caatagagtt attaaggtcg tatttagagg gagctcttta
    58801 taaggttgtg atggctagtg atggtgagga ggctttttct ttatttcagc aacatcagat
    58861 tgatttagca atcatagata ttacgctacc taaaatagat ggatatgagt tgacgcgact
    58921 cattcgtcaa gatagtcaga ttcctattat tatgctagca gctaagacaa cagatatgga
    58981 cagaatatta ggattgaata ttggagcaga tgattttatc acgaaaccgt ttaattcttt
    59041 ggaggtttta gctcgaatta attcgcaact gcgtcgttac tatgaattca atagtcttgc
    59101 caaacctaaa aatcaattta taaagatagg ggaattggaa ctagatgagg aacatgttga
    59161 actaactaaa aatggtaaac atatcaagtt gactgctaca gaatttaaga ttcttcatat
    59221 cttaatgtca tcaccaggaa agatttatac taaaacacaa ctctatgaga agattaatgg
    59281 taggtattta gaaggtgatg agacaacgat tatggtgcat atttctaata tccgtgacaa
    59341 aatcgaagat gatagtaaat atcctaaata tataaaaaca cttagaggag tagggtataa
    59401 aatcgaaaaa ccgcagtatc ttgtttaaaa acataatggc tagtgagagc cattttttag
    59461 tgaagatatt tcttggtttt aagctttcct ttttgctata ataagtaaaa ttggagggga
    59521 gagatatggc aaagaccgct cttaatattc cttttacagt tgaagggata gaaaattatt
    59581 atcaaaatat aaaaatgata ctgtctttag cagataaatg gagcatacct aaagatgatc
    59641 tattactctt tttaaaggga ctcaaggcta ttgatgatat aaaagttggc tctcctattt
    59701 ttaagtattt ttggacttct ttaagtttac aagcacccct caaagcttta gaattcgtgt
    59761 tagagcaggc taaaatgcca actgaattgt caggagaatt atcagaaaca caatacttgg
    59821 tcgcacagtt ttcagatgag ttagctccgc atgatgattt ctggatagcc ttatcacaag
    59881 ttatttatga ttcttttcct gggaatagtc tagccgaaga tactgtatta aataggaaac
    59941 tccatcaatt ccgctatttg atttctagtc agcaagcaca gtatgttcga cgatacttta
    60001 aagatgttgg tatgactgat agagatgctt tagtaaacta cctatcatgt ttaagagaac
    60061 cagatagtat cgcctactat gaatctgctc gcttgcataa taaacgtcgg agaaatggtg
    60121 aaatctttgg tttcccagat gatgagccgg ttattaatag taagctttta atttcattcc
    60181 atacggaatt tattattgat gacaaaggaa actttttaaa cgagattgac gcagaagtaa
    60241 tcacacgtaa tggcattatc aacggtgcta gctttaatta tgcttttaaa aataatacac
    60301 gtcataaaga attagatgtt gatcctgtaa agttagaccc aaaatttcga aacgatatga
    60361 ctagaggtta tagatcgcca aatctatcta gaaggaaatg gtttttcttt aaagaagagg
    60421 attacgattg tagttatttc aataaaaaag gatattacgc atttggaagg cgtagtgcta
    60481 agcaatcggt tgataaacaa gtcaaatact tgaagaaggc tgtgcagaaa atgcgcatca
    60541 actgatacat atactattaa aagataccat gatactatgg tatcttttaa tttgcggtat
    60601 taatgtcaaa tggtttttat tgaccacata tttttataaa cgagtggtca aaattgaaaa
    60661 agtgtctagc ttatgttaaa atacaattga ataatagaaa gaaggtgctg tatgattaag
    60721 aaaaataaag tttttttagg agttctttta gtccttgttg ttatccttgg aggtggtgta
    60781 ttattttacc aatcacaatt ccaaaagaca actaatcaag cgttagctat tgcttataaa
    60841 gatgctaaag tggctaagaa ggatgtcata catcaaaaaa ttgacaaaga atttgaaaac
    60901 tttagaggga gctacgaaat tgaattcaat accaaaagcg cagaatacag ctatcatgta
    60961 gatgctaaga ctggacaaat ccttgagcgg gacatggata ataatggctt ttcaaaatcg
    61021 acttctcagt caagttcatc gtcaagtcaa aaatctcata aaataagtca agaagaagcc
    61081 aaaaagattg catttaaaga tgctaatata gaggaatcag aagtcagtaa tcttaagatc
    61141 aaagaagaaa ttgaaaatgg aaaatcagtt tatgatattg actttgtgga cctgaaaaat
    61201 aaaaatgaag ttgattatca aattgatgca gaaactggaa aaatcattga acgttctaga
    61261 gatcatatga atgattaatt taaataagat ataaaaaaac gaaggtctaa aaggccttcg
    61321 ttttaattac tcagtagctt gctcccaacg ttttgaaagt ttacgtgaaa aacttgagat
    61381 gataaagttt gtaatgaaat agacagcagt tataatagcg tagagtgtaa aaacttgtcc
    61441 agcttcgaaa tatcttccca ttagaatttg actttttccg aagatttctt gaatagctat
    61501 aacagaataa agtagtgatg tatcttttat aaccgttaca aactgagaaa taatggctgg
    61561 caacattttg cgaaaagctt gtggaaaaat aataataatg aaaacttgca aataagtgaa
    61621 tccttgagaa agtccagctt ctgtctgtcc tttatcaact ccatttaaac ctccccgaat
    61681 aatttctgct aaagcagcgg aggtaaaaat tgtaaacgaa gtaataccag cgctgactga
    61741 tttcatctgg aatactaaga aaatgataaa aatccaaagt agatttggaa catttctgac
    61801 aaattcgata tagatactag ctattaattt ccagatagga tttttgctat ttctcataat
    61861 tgctaataga gttccaaaaa acattgatag taaaatggaa ataaaagata tatataaggt
    61921 taaaccgaac ccttgaagga gaaatagtat atttgttagt gttaagactg atgacatatc
    61981 aaacctcctt aagaataagt tttcttattt aattcttctt gttttcttgc ccagcttgct
    62041 agtgggaaac aaataacaaa atagaagata gcagctcctg caaaagctgg gatataatta
    62101 gtcgtatcat aagcccaggc cttagctaaa aacatgatat cggctccaga aatgattgct
    62161 acggtagagg tattcttgat taggttaaca acttggttgg tcattggtgg taaaattgtt
    62221 ctgatagctt gaggcaaaat gataagttgc atggtttgat tagctgtgaa tccttgagct
    62281 aaagcagcct ctgtttgtcc tttagggaca gcttcaattc ctgagcgaat aacttcagaa
    62341 atataggcac catggtatag gccaacgcac aaaacagcgg tgaaaaaagc actaatcata
    62401 atatgaccat tactaatgat tgctagtcca tagtagacaa aaaccatttg aaccaaaaga
    62461 ggtgtgtttt gaaaaacttc aacatagata cgtgatatca acttaccaac tttactttta
    62521 gaagttgata aaccaccaga gagaattcct aatatcagag caagtaggag tgcaccaaac
    62581 gaaataccta gagtatataa gaagccttta aagaataggt caaaatggtt aaagaaagcg
    62641 ccccaccttg aaatggcaaa aggactgatg tttaataaag tcataaaaaa tcctccctat
    62701 tcttttttta ctttagcggg tttaagctta aatttttgat aaatgtgttt taaactacca
    62761 tctttttccc atgataccag taacttatta acgtagggaa tcaagacttt gtcctgttta
    62821 ctggttgcaa taccgtattc ttgcgtattg aacccttctt ttaatatggt tgtatgaggg
    62881 ctaaggtagc cgctaaggat agatttatca actgaaaaag catcaatacg attagcgtac
    62941 agcgatatcg ctaattctgg gaaactccct aattgagcat agctaaattt tagcttgtgt
    63001 tctttagcat atttttctaa attgactttt gtagtggcac cttgagccac accaatgtgt
    63061 ttaccatcca attcttttat agttttgata tgactatttt tacgcaccaa aaatccaatt
    63121 tcatcgtgat aataagcttt agaaatatta tagttagctt ttctttcggg cgtgatagtg
    63181 tatgttgcga ttagaatatc aatttgccca ttgtccatca gaggctctct ggtttgtgca
    63241 gttgtcggta caaaaactgg tttgacgcct aaggatttgg caattttttt ggcaatatca
    63301 atttccattc cttcgtattg attcgtctca gcattataat aaccaaagtt aggcacatct
    63361 tgcttgactc caactttaag gacacccgat tttttgattt ctgataattg attagctgac
    63421 gttgagttgg gataaaataa caggaaaatg aaaatacagg ataatagata gagcgttaat
    63481 ctttttcgtc tcagcatatt tcgtcctcct aaaggtcagt actggtatgg ttgataatgt
    63541 ttcttaaaaa ttgtttggca cgtggttctg tgggattatc aaaaaagtct tgcacatccg
    63601 tagtatctac taaaatttcc ccgtcagcca taaaaataat acgatcagca acttctcgtg
    63661 cgaatcccat ttcatgagtt acaacgacca tattcattcc gtcatttgcc aatttttgca
    63721 tcactgaaag aacgtctccg atggtttcgg gatcaagggc agaggtaggc tcatcaaaga
    63781 ggaggagttt tggatgcatg gcaagccctc tagcaatagc aattctttgt ttttgaccac
    63841 ctgatagcat agatgggtag gaatcttttc tttcccacat attgacaaat tttaaatatt
    63901 tttcagcaat ttccatagct tcttttttag attgttttaa tactttaata ggagctaatg
    63961 taatattttc taacacggtt ttgtggggat aaaggttaaa atgttgaaaa accataccaa
    64021 cttctttacg gaggttgacc aattctttag atgaaatatt tgctaattca tgtccattca
    64081 cgaccaaact accatcgtca atagattcga gagcgttcat tgttctaatg agtgttgatt
    64141 tacctgatcc agaaggtcct aatagaacaa ccacttgccc tggctctatt tccaaattaa
    64201 tctgacgaag ggcgtgataa tctccataat acttgttaac attcttataa gatatgagtg
    64261 acataagaac ctcctaaaat aacagtttat tgttaggtta cctaacatta tagataagtg
    64321 tatcacctgt ttgtgagctt gtcaataaat tcagaatatt aaagagtagg attgatttaa
    64381 acattgctat ttttagagcg gtatttctag taaaaatatt acgttctcgt atcatttatt
    64441 tttcagttta ataggatgta ttgaaagttt ccaattaact aggaaatctg ttataattag
    64501 tgataatagt ggtgctttag gcccatatta tcatattttc ggtcactttt atttttcagt
    64561 ttatggatgg aagtgggaga ccagaaagta atagaggtaa aatatgaaaa aaatacttat
    64621 cgttgatgat gaaaaaccaa tttcagatat tatcaaattt aatttaacca aagaaggata
    64681 tgaaacggcg acagctttcg atggccgtga agctttagta caatatgcag aatttcaacc
    64741 agatttaatc attttggatt taatgctccc tgaactagat ggtttagaag tggctaagga
    64801 agttcgtaaa accagccata ttcctattat tatgctttcc gcaaaagaca gtgagtttga
    64861 caaagtcatt ggtttagaaa tcggtgcaga tgattatgtt actaaacctt tttcaaatcg
    64921 tgaacttttg gcacgggtca aagcacattt acgacgtact gaaaatattg aaacagctgt
    64981 agcagaagaa agtgctcaaa atgcttcttc agatattact attggtgagt tgcaaatttt
    65041 accagacgcc tttattgcta agaaacgtgg cgaagaaatt gaattaacgc accgagaatt
    65101 tgaattactt caccatttag cgacacatat aggacaagtc atgacacgcg aacatttatt
    65161 ggaaacggtt tggggatatg attattttgg agatgtccgt acagttgatg tgacggtgag
    65221 acgtttacgt gaaaagattg aagatactcc tggtcgtcca gaatatattt tgactcgacg
    65281 tggtgtaggt tattacatga agtcttatga ataatagtgc tgcaaatatt aggtcgtttg
    65341 agctagcctt acttttttta ctagtttttg ttgctgtcta ttttgtttat ctagcggtta
    65401 gagattttaa aatgtctaaa gatatccggc ttttgaattg gaaagtcaga gatttgatag
    65461 caggaaatta ttcagatagt attctaattc aaggtgatgc agatttggta gaattagggg
    65521 aaagtctaaa tgatttatct gatgtcttcc gaatggcaca tgataactta gaacaagaaa
    65581 agaatcgctt agcgagtatc ttaacttata tgactgacgg tgtgctggct acagatcgtt
    65641 caggaaaaat cgttatgatt aatgagacag cccaacagca gttcaatcta gcatatgatg
    65701 aagctttatc aatgaatatt gttgatatgt tagggtcggg aagtccatat tcgttccaag
    65761 atttagtctc taagacgcca gaggtagttc ttaatagacg tgatgaaaat ggtgaatttg
    65821 ttaccttacg gattcgattt gctttgaacc gtcgtgaaag tggttttatt tccggtttag
    65881 ttgcagtatc acacgatgct actgagcaag agaaagaaga gcgtgaaaga agactttttg
    65941 tatctaatgt ttctcatgaa cttcgtacac ctttgacatc tgtaaaatct tatttagaag
    66001 ctttagatga gggagcgctc aatgaagaag ttgctccaag cttcattaag gtatctctag
    66061 atgaaacaaa tcgtatgatg cgtatgatat cagatttatt aagtttatcg cgcattgata
    66121 atgaagtaac gcatttagat gttgaaatga cgaattttac agctttcatg acctcaattt
    66181 tgaatcgatt tgatcagatt agaaatcaaa aaacagtcac aggaaaagtt tatgaaattg
    66241 tcagagatta tcctcttaag tcaatttggg tggaaattga tacagataag atgactcaag
    66301 tgattgataa cattttaaat aatgcagtca agtattcacc agatggtggt aagattacag
    66361 ttaatctacg cacaactaaa acgcagatga ttttatcaat atcagaccaa ggcttaggta
    66421 ttcccaaaaa agatttacct ctcatttttg atcgttttta tcgtgttgat aaggcgagaa
    66481 gtcgtcaaca gggtgggact ggacttggtt tgtcaattgc aaaagaaatt gttaagcagc
    66541 ataagggatt tatttgggct aagagtgagt atggtaaagg gtctactttt acaatcgtct
    66601 tgccttatga taaagatgct gtaacttatg aagaatggga ggacgttgaa gattaaaaca
    66661 tgtcagaaat aggatttaaa tatagtatct tagcttcagg ttcaactgga aattgttttt
    66721 atattgagac accccaaaaa cgtcttttaa ttgacgctgg tttaacaggc aagaaagtga
    66781 ctagccttct tgcagaaatt aatcgtaaac cagaagattt agatgctata ctagttaccc
    66841 atgaacattc tgaccacatt aaaggtgttg gcgtattggc taggaaatat caccttgata
    66901 tttatgccaa tgagcagacg tggaaagtta tggatgagcg taacatgtta ggcaaagttg
    66961 atgttagtca aaagcatgtt tttggacgcg gtaaaacctt aactttcggg gatttagata
    67021 ttgagagctt tggtgtaagt catgatgctg tggatcccca attttaccgt atgatgaaag
    67081 atgataagag ctttgtgatg ttgacagata caggatatgt tagcgaccga atggcaagct
    67141 tgattgagaa tgcagatggt tatttgatcg agtcaaatca cgacattgag attctacgtt
    67201 cgggttctta tccttggaca cttaagcaac gtattttatc agataaagga catttatcta
    67261 atgaagatgg ttcagaaacc atgattcgca ctataggtaa ccgtactaaa cacatctatt
    67321 taggacactt gagtaaggaa aataatatta aggaacttgc tcacatgact atggaaaaca
    67381 atttaatgag agctgacttt ggtgtgggca cagatttttc tgttcacgat acttcacctg
    67441 attcagctac cccactgacg aggatttaag attgaaaaag attttttaca ttagtttagg
    67501 atttattagt ttagggatag gcattgcagg tattgtcttg ccagtagttc caacgactcc
    67561 attagtattg ctttcagctt tttgcttttc gagaagctct gaaaaatttg atatttggtt
    67621 gcgtcaaact aaagtttata aatactacgc tgcagatttt gttgaaagca gaagtattgc
    67681 cccagcgcgt aaaaagtcaa tgatttggca aatttatatc ttgatgggga tttcaatata
    67741 ttttgctcct ctaatgtggc tcaaactggg attattgatt ggaactattg taggaactta
    67801 tgtgcttttt tatgtagtac ctgataaaat tgatgaatag ttaacaagag actaggtgtg
    67861 tatcctagtc ttttgttgaa tattcaaata tgtaattaag gaaacgtttt ctttggaaga
    67921 taagtttaac ttccaaaatg tttgattatt tgtcaactgt ggtataatgg tatttgttta
    67981 atattttatc tatgattgaa atcaaggaga aaaaatgaaa gaattaagat caaagctgga
    68041 aaaagactat ggtattgttt ttgctaatca agaattatta gatacagctt ttacccatac
    68101 atcatacgca aatgagcatc gcctcctaaa catttcacat aatgaacgtt tggaattttt
    68161 aggagacgct gttctacaat tacttatttc acagtatctt tttacaaagt accctcaaaa
    68221 ggcagaaggt gatttatcaa aattacgttc aatgattgtt cgtgaggaaa gtcttgcagg
    68281 tttttcacgt ctatgtggct ttgatcacta tattaaactt ggaaaaggag aagaaaaatc
    68341 tggtggacgt aatcgtgaca ctattttagg agatttattt gaggcttttt taggagcttt
    68401 attattggat aaaggtgttg aagtggttca tgcctttgtt aacaaggtaa tgatccctca
    68461 cgttgaaaaa gggacctatg agcgtgttaa agattacaag acaagcttgc aagaattact
    68521 tcaatctcat ggggatgtaa aaatagatta tcaagttact aatgaaagtg gtccagcaca
    68581 tgcaaaggaa tttgaggtga ctgtatctgt caaccaagaa aatttgagcc aaggaattgg
    68641 acgatcaaag aaagctgctg aacaagacgc tgccaaaaat gctttagcaa cgttacaatg
    68701 aggaaatcat gtttttaaaa gaaattgaaa tgcaaggctt taagtccttt gcggataaaa
    68761 ctaaggtaga atttgatcaa ggagttactg ctgttgtagg tccaaatgga tcaggtaaaa
    68821 gtaatatcac agagagttta cgttgggcat tgggagaatc aagcgcaaaa agtttacgag
    68881 gaggtaagat gccagacgtc atatttgctg gtacagaaaa tcgtaagccc cttaattatg
    68941 cgcaagtgag tgttacttta gataacagcg accactttat cgaaaatata gctgacgaag
    69001 ttcgagttga acgtcgtatt tttcgaaacg gagatagtga atatttaata gatggacgca
    69061 aagttcgctt acgtgatatt catgatttat ttatggatac tggattaggg agagattctt
    69121 tttctattat ttcccaagga cgcgttgaag ctatttttaa tagtaaacca gaagagcgcc
    69181 gtgccatatt tgaagaagcc gctggtgttc tgaaatacaa aacacgtaag aaagaaacac
    69241 aatccaaatt agaacaaact caagggaact tagatcgctt agaggatatt atttatgaat
    69301 tggacatgca agtccaacca ttagaaaaac aagcaagtat tgctaagcgt tttttagtat
    69361 tagatgagga acgacaagga ctccacttat ctattttgat tgaagatatt ttacaacatc
    69421 aatcagattt aactactgtt gaggaaaagc tattgacagt aagagaagag ttagcaactt
    69481 attaccaaca acgtcagtcc cttgaagatg aaaatcaatc gttaaaacag aaacgtcacc
    69541 atttatctga agaaattgag gttaaacagt tagctctttt agacgttaca aagttaaagt
    69601 cagatttgga acgccaaatt gatctgattc gtttagagtc aagccaaaag gctgaaaaga
    69661 aagaagaggc aggtcaacgc ttagcagagt tagaagttaa agctaaagat tgtaatgacc
    69721 agattaccca aaaaaatata gagttaacta ctctgtcgga aaaaatagcc caaataagat
    69781 cagaaattgt tagtatagaa agttccttag agcgtttttc aactaatccg gatcaggtta
    69841 ttgaaaaact tcgagaagac tttgttacct tgatgcaaga agaagccgac acttcaaacg
    69901 ctttaacagc tttactagct gatatagaga atcaaaaaca agcttctcaa gctaaatctc
    69961 aagaaataca agaagttagc aaaaatttag aagtattaaa aagtaacgca aaggtagcat
    70021 tggaacgatt tgaggctgca aagaagaatg ttcgtcagtt attatctcac taccaagatt
    70081 taggtcaaac cttgcaaaat ctagaaggtg agtataagaa tcagcagtca attttatttg
    70141 atcatttaga tgaaatcaaa tcaaagcaag cacgtatttc gagtttagaa tctattttga
    70201 aaaatcatag taatttctat gctggagtga agtcagttct acaggctaag gaccagcttg
    70261 gaggtatcat aggtgctgtt agtgagcatc ttagctttga caaacattac caaacagcat
    70321 tggagatagc tttgggaggt agcagccaac acatcattgt tgaagacgag agtgctgcca
    70381 aacgttcaat tgctttttta aagaaaaacc gccaaggtag agcgacattt ctgcctttaa
    70441 ctacaataaa accaagagaa ttagcacaac attatctctc aaaattacaa agttctcaag
    70501 ggtttttagg aatagctagt gaattggtaa cctatgatca acgcttgtca aacattttta
    70561 aaaataactt agggttaact gctatttttg atacggttga taatgctaat gtagctgcaa
    70621 gacaattgaa ttatcaagta cgtttagtaa ccttagatgg aacagagtta cgccctggag
    70681 ggtcgtactc agggggagca aatcgtcaaa ataatactgt ttttatcaag cctgaattgg
    70741 ataatctaaa aaaagaattg aaacaggcac aaagtaaaca gttaattcaa gaaaaagaag
    70801 ttgccgcatt gttagagcaa ctcaaagaga aacaagaaac cttagcacaa cttaagaatg
    70861 atggagaaca agctcgtctt gaggaacaaa gagctgatat agagtatcaa caactatcag
    70921 aaaagcttgc tgatttaaat aaactatata atggcttgca attaagcagc ggggcgttgg
    70981 aacaaactac ttctgaaaat gaaaaaaatc gtttagaaaa agaacttgaa caatttgcta
    71041 ttaaaaaaga agagttgaca acttcaattg ctcaaattaa ggaagataaa gattcaattc
    71101 aagaaaaagt caataatcta acaacattat tgtcagaggc acagctagaa gaacgtgatt
    71161 tgctaaacga gcagaaattt gaacgtgcca actgtacacg tctagagatt accttgtctg
    71221 aaataaaacg tgacattagt aatttacaaa cgttactaag ccatcaggac agtcaattag
    71281 acaaagaaga gcttccacgt atagaaaaac agttgctaca agcaaataag agaagagaaa
    71341 atgacgaaga aaagcttgtg agtctccgtt ttgaattgga ggattgtgaa gctgctttag
    71401 atgatttagc tgcttctctg gctaaagaag gacaaaaaaa tgaaagcctt attcgtcagc
    71461 aagctcaatt agagtcacaa tgtgaacaat tatctcaaca attgatgata ttctcacgtc
    71521 aattatcaga agactatcag atgacattgg atgaagcgaa ggtaaaagca aatgtcttgg
    71581 aagatatttt gatggcgcgt gaacaattga aatctctaca agctaaaatt aaagctttag
    71641 gacctgttaa tattgatgca attgctcaat ttgaagaagt tcatgaacga ttgaccttcc
    71701 ttaacacaca gcgagacgac ttagttcatg ccaaaaattt attactagag acgattacag
    71761 atatggatga tgaagttaag actcgtttta aatcgacatt tgaagccatt cgtcatagtt
    71821 tcaaagaaac ctttgtacag atgtttggtg gaggttctgc gaaccttatt ctgacagaag
    71881 gtgatttatt atcagctggg gttgatattt cggttcaacc tcctggtaaa aaaatccagt
    71941 ctttaaactt gatgtcaggt ggtgaaaaag cattatcagc gttagcatta ttatttgcca
    72001 ttatccgtgt taagacaatt ccatttgtca tcttggatga agttgaggcc gctttagatg
    72061 aagcaaatgt taagcgattt ggcgattatc ttaatcgatt tgataaatca agtcaattta
    72121 tcgttgttac tcaccgaaaa gggactatgt ctgctgctga tagtatctat ggggtaacga
    72181 tgcaggaatc aggcgtttcc aaaattgttt ctgtaaaatt aaaagaagct caagaaatga
    72241 ccaattagaa aacagaaggg gatcccttct gttttttgga ttttcagcct tattttatgt
    72301 tacaatagta aatgtaaacc ctttagttag gagattccat gataaaatta gtagcaaccg
    72361 atatggacgg tacattctta gatgaaaacg gtacctatga taaaaaacgt ttagcaaatg
    72421 tgctaaaaaa atttaaagaa caaggaattg tctttacggc agcaagtggg cgttccttat
    72481 tatcacttga acaattattt gctgactttc gtgatcagat ggcttttata gcagaaaacg
    72541 gaagtgcagc agtcttattt aatcgcttgg catatgaaca gcacttgagt cgagaacaat
    72601 acttagatat tattgaccat ttgagtaaga gcccttatat ggagaataat gaatatgtct
    72661 tatctggcaa ggatggtgca tatattctaa gtgatgccaa tccagattat attgaattca
    72721 tcactcatta ttatgataat ttgcaaaaag tttctcactt tgaagatgtt gatgatattg
    72781 ttttcaaagt aacggctaac tttactgaag agactgttag acaagctgag gagtgggtaa
    72841 atcaagctat accgtatgcc acagcagtga caacaggttt taaatccatt gatattattc
    72901 taagctcagt taataaacga aatggtttag agcacctctg tgaacaatac ggtattagag
    72961 cagaagaagt cttatctttt ggtgacaata ttaatgattt agaaatgcta gagtggtcag
    73021 gaaaagctat cgcaacagaa aatgctagac cagaagttaa agagatagca gattgtatta
    73081 taggtcatca caatgatcaa gctgtaatgg cttatttaga aagtatggtg gattaatgtt
    73141 ggatattaaa atacttgcct tagatttaga tggcacttta tttacaacag ataagaaagt
    73201 ttcagaagaa aataaagtgg ccttaaaagc agctagagaa aaaggtataa aagttgttat
    73261 tacaacaggg cgtcctttaa aagcaattgg taatttgtta gaggacctag agttagtatc
    73321 tgatgaagat tacagcatta cttttaacgg tggtttagtt caacaaaata cagggaaaat
    73381 cttagctaaa actgcaatga cacgacaaga agtggaagat atccatgagg agttgtatca
    73441 agtgggtttg ccaacagata tcttaagtga gggaacggta tatagtattg ctaacaaggg
    73501 acaccattct caatatcatt tagccaaccc actcttagaa tttatagaag ttgatgattt
    73561 agagcaagtg ccaaaggatg tagtttataa taaaattgtt agtgttattg atgctacata
    73621 tcttgatcaa caaattgcta aacttcctga tagacttaaa gttgactatg aaatgtttaa
    73681 atcacgtgat atcattttag aattgatgcc taaaggtgtt cataaagcag tcggcttaga
    73741 actactgaca aaacaccttg ggttagatag tagtcaagtt atggcaatgg gagatgaagc
    73801 aaatgattta tcaatgctag agtgggcagg cttaggagtt gcaatggcta atggtattcc
    73861 agaagctaag gcaacagcaa aagcgacaac tatttgtaat aacgatgagt ctggagttgc
    73921 cgaagcagtt gggaaatata ttttaagtga ggaaaattaa tgggattatt tgaccgccta
    73981 tttggacata agaaaaaaga taaagaacta gaaattgaag catcagaatc tgttgtttta
    74041 gaaaatgaag attcagttat tgataaagag gaaagcagca acttttcgaa ggagtcaact
    74101 ttgaatcgaa cctctgaagt ccctgtcgca gaagatgatt ctttcttaga attagaaaga
    74161 gatacagcat tgtcagaatc acatcaacca gttacctctg aaattcatcc tttagagtca
    74221 gaagatacct ctgaaattcc tgtaaaagaa gacgatagtt ttttagaact agaagatagg
    74281 gctaagacaa aggtggcaga tacttctgag gtaggaaatg tagttcctga ttccacgact
    74341 ctttctgaca atgtttctgc taaatctgag gcatctttta gtgatgagga gcaatcaagt
    74401 gatagtcagg cttcagagca gttttcagaa actccccttc aagaagagat gtctagtgga
    74461 aaaactgagg tacaaacaga gtcagaggat acatctgcgg ctgatgcttt tttagcagat
    74521 tactatgcta aacgtaaagc aattgagaaa gaaatctcgt ctaattcgct gtcaacagat
    74581 gagtcagaac tttctgaggc acaggaagtt ttatcgcaat cacaggcaga cactataaaa
    74641 gctgagagtc aagaggaaaa atataatcgt agcctgaaaa aaacacgcac aggttttagt
    74701 gcacgcctta atgctttcct ttctaacttc cgtcgtgttg acgaagagtt ctttgaagaa
    74761 ttagaagaaa tgttaatctt atctgacgtt ggggtaaatg ttgcaacaca gttaactgaa
    74821 gaccttcgtt atgaagcaaa attagagaat gccaagaaat cagaggattt aaaacgtgtt
    74881 atcgttgaaa aattagttga aatctacgaa aaagatggta tttataatga ggctatcaat
    74941 ttccaagaag gtttaaccgt aatgcttttt gtaggtgtta atggtgttgg aaaaacgaca
    75001 tctataggta agttagctca tcaatacaag tctcaaggga aaaaagttat gttagtagca
    75061 gctgatactt tccgtgcagg tgctgtggct caactagtag aatggggacg ccgtgttgat
    75121 gtaccagttg tcactggaga aaaaaaagca gatccggcta gcgttgtctt tgatggcatg
    75181 gaaaaagccg ttgctcaagg cgttgatgtc ttgttaattg atacagcagg acgtttgcaa
    75241 aataaagaaa accttatggc agagttggaa aaaattggac gtattattaa gcgtgtcgta
    75301 ccagatgctc cccacgaaac tttgcttgct ttagatgcat ctacaggaca aaatgcttta
    75361 agtcaagcta aagagttctc gaaaattaca ccattaactg gccttattct gactaaaatt
    75421 gatggtactg ctaagggtgg tgtggtactg gctattcgtc aagaactaga tattcctgtt
    75481 aaattcattg gtttcggcga aaaaatcgat gatattggtg aatttaattc agaagatttc
    75541 atgcgtggcc ttctggaagg aattttataa gaaaaaagaa ctttcattta gaaagttctt
    75601 tttatagtac gacgggcatg tcgtacatct gaggtgcaag tcctctgtgg gcacccgcta
    75661 ccggtgaacc caatagcgac tcccaagctt gactatcgtg aggtagcggg gagaggaagg
    75721 gatagcgaaa tcatggctct a
//
LOCUS       DATRSG010000002        65619 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRSG010000002 DATRSG010000000
VERSION     DATRSG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65619
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.002"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..567)
                     /locus_tag="VBD89_000072"
     CDS             complement(<1..567)
                     /locus_tag="VBD89_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134635.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKITDTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYILIGKYC"
     gene            complement(588..899)
                     /locus_tag="VBD89_000073"
     CDS             complement(588..899)
                     /locus_tag="VBD89_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO7134636.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            1090..1797
                     /locus_tag="VBD89_000074"
     CDS             1090..1797
                     /locus_tag="VBD89_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO7134637.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVKKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQDVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            2075..3223
                     /gene="nagA"
                     /locus_tag="VBD89_000075"
     CDS             2075..3223
                     /gene="nagA"
                     /locus_tag="VBD89_000075"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO7134638.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKAVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            3341..3883
                     /locus_tag="VBD89_000076"
     CDS             3341..3883
                     /locus_tag="VBD89_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO7134639.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAVLTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVS"
     gene            4196..5110
                     /gene="glyQ"
                     /locus_tag="VBD89_000077"
     CDS             4196..5110
                     /gene="glyQ"
                     /locus_tag="VBD89_000077"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO7134640.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            5110..5751
                     /locus_tag="VBD89_000078"
     CDS             5110..5751
                     /locus_tag="VBD89_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7134641.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            5755..7794
                     /locus_tag="VBD89_000079"
     CDS             5755..7794
                     /locus_tag="VBD89_000079"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO7134642.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQHLADKAGLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRIGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDSSLFENNQEKALYQAILSLELTEDIHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            7806..8063
                     /locus_tag="VBD89_000080"
     CDS             7806..8063
                     /locus_tag="VBD89_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO7134643.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            8152..8415
                     /locus_tag="VBD89_000081"
     CDS             8152..8415
                     /locus_tag="VBD89_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO7134644.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            8530..10038
                     /gene="glpK"
                     /locus_tag="VBD89_000082"
     CDS             8530..10038
                     /gene="glpK"
                     /locus_tag="VBD89_000082"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO7134645.1"
                     /translation="MSSEEKYVMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            10051..11880
                     /gene="glpO"
                     /locus_tag="VBD89_000083"
     CDS             10051..11880
                     /gene="glpO"
                     /locus_tag="VBD89_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO7134646.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            11892..12590
                     /locus_tag="VBD89_000084"
     CDS             11892..12590
                     /locus_tag="VBD89_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7134647.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            12678..14015
                     /locus_tag="VBD89_000085"
     CDS             12678..14015
                     /locus_tag="VBD89_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7134648.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDTVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGLYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            14006..15436
                     /locus_tag="VBD89_000086"
     CDS             14006..15436
                     /locus_tag="VBD89_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7134649.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     LSDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHDISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            15561..17546
                     /gene="tkt"
                     /locus_tag="VBD89_000087"
     CDS             15561..17546
                     /gene="tkt"
                     /locus_tag="VBD89_000087"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7134650.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVAARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            17758..18063
                     /locus_tag="VBD89_000088"
     CDS             17758..18063
                     /locus_tag="VBD89_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7134651.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            18083..18817
                     /locus_tag="VBD89_000089"
     CDS             18083..18817
                     /locus_tag="VBD89_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134652.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            18821..20425
                     /locus_tag="VBD89_000090"
     CDS             18821..20425
                     /locus_tag="VBD89_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134653.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPISYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(20633..22019)
                     /locus_tag="VBD89_000091"
                     /pseudo
     CDS             complement(20633..22019)
                     /locus_tag="VBD89_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            22160..22963
                     /gene="proB"
                     /locus_tag="VBD89_000092"
     CDS             22160..22963
                     /gene="proB"
                     /locus_tag="VBD89_000092"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO7134654.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     KGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            22973..24226
                     /locus_tag="VBD89_000093"
     CDS             22973..24226
                     /locus_tag="VBD89_000093"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO7134655.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGTKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASETDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            24309..25256
                     /gene="rsmH"
                     /locus_tag="VBD89_000094"
     CDS             24309..25256
                     /gene="rsmH"
                     /locus_tag="VBD89_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO7134656.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGTYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDIAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            25271..25597
                     /gene="ftsL"
                     /locus_tag="VBD89_000095"
     CDS             25271..25597
                     /gene="ftsL"
                     /locus_tag="VBD89_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO7134657.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            25601..27859
                     /locus_tag="VBD89_000096"
     CDS             25601..27859
                     /locus_tag="VBD89_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7134658.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPVLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            27861..28871
                     /locus_tag="VBD89_000097"
     CDS             27861..28871
                     /locus_tag="VBD89_000097"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO7134659.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLVVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            28969..30312
                     /locus_tag="VBD89_000098"
     CDS             28969..30312
                     /locus_tag="VBD89_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7134660.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLVIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVEEKRRKERRASNRAKGRAERKAKKQSF"
     gene            30450..31262
                     /locus_tag="VBD89_000099"
     CDS             30450..31262
                     /locus_tag="VBD89_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7134661.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            31357..32160
                     /locus_tag="VBD89_000100"
     CDS             31357..32160
                     /locus_tag="VBD89_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7134662.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPHHLTDEIPGGVR"
     gene            32160..32903
                     /locus_tag="VBD89_000101"
     CDS             32160..32903
                     /locus_tag="VBD89_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7134663.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            33025..33249
                     /locus_tag="VBD89_000102"
     CDS             33025..33249
                     /locus_tag="VBD89_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO7134664.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIFWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            33318..34232
                     /gene="trxB"
                     /locus_tag="VBD89_000103"
     CDS             33318..34232
                     /gene="trxB"
                     /locus_tag="VBD89_000103"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO7134665.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            34390..35850
                     /locus_tag="VBD89_000104"
     CDS             34390..35850
                     /locus_tag="VBD89_000104"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO7134666.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            35847..36668
                     /gene="nadE"
                     /locus_tag="VBD89_000105"
     CDS             35847..36668
                     /gene="nadE"
                     /locus_tag="VBD89_000105"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO7134667.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKDLYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            36781..38115
                     /gene="pepC"
                     /locus_tag="VBD89_000106"
     CDS             36781..38115
                     /gene="pepC"
                     /locus_tag="VBD89_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO7134668.1"
                     /translation="MSELTQTFTDKLLADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDISDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(38162..40402)
                     /locus_tag="VBD89_000107"
     CDS             complement(38162..40402)
                     /locus_tag="VBD89_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7134669.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNNSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTADDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNNN
                     TAPNGN"
     gene            complement(40389..40988)
                     /gene="recU"
                     /locus_tag="VBD89_000108"
     CDS             complement(40389..40988)
                     /gene="recU"
                     /locus_tag="VBD89_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO7134670.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            41063..41581
                     /locus_tag="VBD89_000109"
     CDS             41063..41581
                     /locus_tag="VBD89_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO7134671.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            41702..42034
                     /gene="gpsB"
                     /locus_tag="VBD89_000110"
     CDS             41702..42034
                     /gene="gpsB"
                     /locus_tag="VBD89_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO7134672.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            42053..42421
                     /gene="rnpB"
                     /locus_tag="VBD89_000111"
     ncRNA           42053..42421
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBD89_000111"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            42504..43658
                     /locus_tag="VBD89_000112"
     CDS             42504..43658
                     /locus_tag="VBD89_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO7134673.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            43671..45158
                     /locus_tag="VBD89_000113"
     CDS             43671..45158
                     /locus_tag="VBD89_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134674.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDIEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVTIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQAKKTQEEQSSSNKVSDTQTTEQPNVTNGQSSSSAATINNQ
                     AAGTASGNLERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYIL
                     EPVNIINGNGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(45383..45865)
                     /locus_tag="VBD89_000114"
     CDS             complement(45383..45865)
                     /locus_tag="VBD89_000114"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO7134675.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQQINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            46054..47661
                     /locus_tag="VBD89_000115"
     CDS             46054..47661
                     /locus_tag="VBD89_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO7134676.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            47790..48686
                     /locus_tag="VBD89_000116"
     CDS             47790..48686
                     /locus_tag="VBD89_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134677.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELVATLKKEGDTIVYSSHYIEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAETVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            48673..49413
                     /locus_tag="VBD89_000117"
     CDS             48673..49413
                     /locus_tag="VBD89_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134678.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQGLSKLTPTYHLTQVILSPFANSFA
                     VFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            49410..50495
                     /locus_tag="VBD89_000118"
     CDS             49410..50495
                     /locus_tag="VBD89_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7134679.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSIIIRGESKYHMIAWFTMLTYIFYMTVFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATMVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVILKEV"
     gene            50497..51087
                     /locus_tag="VBD89_000119"
     CDS             50497..51087
                     /locus_tag="VBD89_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7134680.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSKQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            51137..51841
                     /locus_tag="VBD89_000120"
     CDS             51137..51841
                     /locus_tag="VBD89_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO7134681.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPTTMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYTKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGISGVKRLYKK"
     gene            52008..52637
                     /gene="gmk"
                     /locus_tag="VBD89_000121"
     CDS             52008..52637
                     /gene="gmk"
                     /locus_tag="VBD89_000121"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO7134682.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            52660..52974
                     /locus_tag="VBD89_000122"
     CDS             52660..52974
                     /locus_tag="VBD89_000122"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO7134683.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            53048..55438
                     /locus_tag="VBD89_000123"
     CDS             53048..55438
                     /locus_tag="VBD89_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO7134684.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEESQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHVIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRNCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDITQDKENRDLRLAIDHEPQNMM"
     gene            55485..56420
                     /locus_tag="VBD89_000124"
     CDS             55485..56420
                     /locus_tag="VBD89_000124"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO7134685.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            56410..57732
                     /gene="rsmB"
                     /locus_tag="VBD89_000125"
     CDS             56410..57732
                     /gene="rsmB"
                     /locus_tag="VBD89_000125"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO7134686.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            57770..58507
                     /locus_tag="VBD89_000126"
     CDS             57770..58507
                     /locus_tag="VBD89_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO7134687.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            58507..60462
                     /gene="pknB"
                     /locus_tag="VBD89_000127"
     CDS             58507..60462
                     /gene="pknB"
                     /locus_tag="VBD89_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO7134688.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            60621..61316
                     /locus_tag="VBD89_000128"
     CDS             60621..61316
                     /locus_tag="VBD89_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO7134689.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            61313..62332
                     /locus_tag="VBD89_000129"
     CDS             61313..62332
                     /locus_tag="VBD89_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7134690.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFKMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            62325..62966
                     /locus_tag="VBD89_000130"
     CDS             62325..62966
                     /locus_tag="VBD89_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7134691.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            63008..64408
                     /locus_tag="VBD89_000131"
     CDS             63008..64408
                     /locus_tag="VBD89_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO7134692.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            64410..64766
                     /locus_tag="VBD89_000132"
     CDS             64410..64766
                     /locus_tag="VBD89_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO7134693.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(64812..65588)
                     /locus_tag="VBD89_000133"
     CDS             complement(64812..65588)
                     /locus_tag="VBD89_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO7134694.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYRLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
BASE COUNT        21410 a        10274 c        13302 g        20633 t
ORIGIN      
        1 acaatatttt cctataagga tataagctat gataagcgaa agaattagaa acatatatcg
       61 aaagaaatat ttcttttctt tcgatttatc gaagttatat gtagcatata ttatggtatc
      121 agttatcttg acagcaataa tactgataaa gattgcaaaa agaacatcta atagagtaat
      181 agggattaga tctggtactg actgttgaag tgcttcagta atgagatatc ctcctaaaaa
      241 gataatcatt tggatagtta gtagtaaaat ggctaaatcc caacgttgta atgtttcttc
      301 tttcttagag attttaggtg tagatttaat aacttggttt ataaagtcgt gcctatcttc
      361 tccaaataca ctaactaaac ttgcttgtcg ctcttgtgct tctaataaca tattgacaat
      421 atcatttcga atgagctcct ggtggtaggg gctaatgcta gcacctcgca aatagacaat
      481 catatctgta taggtttggt tattagagtt atcaattctt ccttctaata gcgtattttc
      541 tttttgtaat tttttcagtt tatccatgct agtttcctct ccaattttta gagtagtttg
      601 tttaatgctt tactaagtga ctcccaattc tgtttaaatt gggctaattg ctccacgcct
      661 tcattggtta acgtataata tttacgttta ggtccttgag gggaaggctt ttgttgtcct
      721 tttatatatt tattgctttg tagccttaag ataatggggt agattgtccc ttcggaaatt
      781 tccccaaaac cataagaatt taattttttt gagatttcat aagcataagt ctcctcttgc
      841 tgtatgattt ttaggatagc tccttcaagt aatcctttta gcatttgcga tggaatcata
      901 ttatatcctt atctatattg taatacaagt tactggttaa ctacattgta atacaaagta
      961 gttgagatgt caaatataac taagttaccg gtagaaaata agagtgatag acgatgattc
     1021 ttctaaaata ggtttgttta tgtcctttag tttatgttat aataatgtga agaaacttta
     1081 ggagtgagta tgaaaataga taaaaaagaa cttttagcac ttattgctag tattatttta
     1141 ttaatttttg cttctgttac gtttttctta tttaaagatc atggcactac tcaaatggat
     1201 actgtagaaa gcagtgttaa ccatgtcagt gattctcaat taactgaggc acaagatatg
     1261 ttagataaat ttgagaagaa accttcagaa aaattattga aggatgtcga acttgcttta
     1321 aataaattgt ccaattcttc taaaaaagaa gctttgcaaa aacgttttaa gaaagcaaaa
     1381 gataagtatc ttaaagacga agcagataag aaagctacca aagatgctac agatttagta
     1441 gaaattctag aacaagcccc ttcagaagaa aatgtgttaa aagctgaagc agcagtcaaa
     1501 aagttaactg tcaaagaatc taaagaagct ttacaaaaac gtattgatac tgttaagaca
     1561 caatatggtt tgattgggaa tcaaactccc tctagttcag tagctgaaac tacagaacaa
     1621 gggacagcta atcctgctag tcaggatact tctagttacg ttaatcagga tgtagcacca
     1681 acttatgagc aaccgcaaac gaataataca ccagttactc caggggttaa caatactgtt
     1741 ccgactccag gaactggtac tgtacctgct actaatggga cgggtgttgc tcagtaaaat
     1801 cctgaaagtc tagttttact agactttttt gctatgcttt ttaattagcc attctcatca
     1861 aaaactataa gagaattagc aattgatagt tttggagaag gaaaagaatt atgagcaaca
     1921 gcaattaaaa ccttaaaagt acaaccaagt gaagtggttt ataacaagtg tatagttctt
     1981 tcttagaata aaccatgact ttttaaaaaa taggtatata ccatttacaa aaatacctta
     2041 aagcgttata atgagagtga atggggggaa gaagatgacg aagtacatca aggctgaccg
     2101 attcttttat gctgatcatg taaaggaaaa cggttattta gaaataaaag ataatcactt
     2161 tggtaaatgg atagaaaata tttctggaca agaggagatt ttagattatt caggttacca
     2221 aatagcacct ggtttagttg atacacatat tcatggtttt gctggcgctg atgttatgga
     2281 ttgtgattca gaaggaattt tgcgaatgag tgctggctta ctatctactg gagtcacatc
     2341 tttcttgccg acaaccctaa cttcagatac aaagcgacta gaagaggcgt ctaaagcagt
     2401 tgcggcagta gcgggcaaag aacaaggagc aaaaattcaa ggcatctatt ttgagggacc
     2461 atattttact gaggaatata aaggagctca aaatccgatt tatatgagaa atccaaactt
     2521 ggaagagttt gctcaatggc agaaggctgc aaaaggcttg attacaaaaa ttgctttggc
     2581 accagaacgt gagggagttg aggaatttgt ctcagcaatc acaaagcaag gtgttacggt
     2641 agctttaggt cattctaatg ggacatataa agaagctaaa aaggcagtga aggcgggtgc
     2701 ctcagtttgg gtacatgcgt ataatggtat gcgtggtttg acacaccgag aaccaggtat
     2761 ggttggtgcg gtttataatt tacctaatac ttatgctgaa ctcatttgcg atggtcatca
     2821 cgttgatcct gttgcttgtg acattttaat gacacaaaaa ggccataatc atgtggcatt
     2881 aataacagat tgtatggcag ctggaggtgc ccctgatgga gattatatgt taggggaatt
     2941 gcccgtggtt gtgtctaatg gaactgcgcg tttgaaagat ggagggaatt tagctggttc
     3001 tattcttaag ctcaaagatg gattaaaaaa tgttgtagaa tggggaattg ctactccgga
     3061 agaggcagtg cgtatggcga gttatgttcc agcagtgtct gtaggcattg ataatgtctg
     3121 tggacagata aaagaaggct atgcagcgga ttttattgta ttagataaac atctagattt
     3181 agttgcaaca taccttgatg gagaaaaacg ttggcaatct tgaaagaact agtttttact
     3241 agttcttttt aatttattaa tttttaatga aatatagttt gacaataaaa ctatttttct
     3301 tataatgttg taatataata caaaacaata ggagtttaca atgtcaatta ttattgggtt
     3361 aatggccgct atgtttatca ttcgtttagc ttatttaaag ctatctatag ctaatgaaaa
     3421 ggccttaaga aaaaatggtg ctaaagaata tggtgtaggt gtttcaaaag caatcacagt
     3481 tcttcatatt atcatttatt tttcatctgt aactgaggct gttctaacaa aagcaagttt
     3541 taattttgtt agcgtaatcg ggcttagctt gatgattttt tctgtgttta tgttacatac
     3601 agtaacacgt ttacttggtc gtatttggac agtgaaatta atggtagata aaaatcatca
     3661 atttgtagat cactggctat ttagggttgt taagcatcct aactatttct taaatattgc
     3721 tcctgaactt ttaggtgtaa ctttattgtg tcatgctaag tatacagcat tatttgtctt
     3781 gccaatttat gcttttgtta tttatttaag aattcgtgaa gaaaacttat tattaaaaac
     3841 gatcattatt ccaaatggaa tcaaaaagtc tagggtatct taagaataag ataaatagta
     3901 gacataaaag tcattttttg ttatactgtc tcagtaagaa gaggtgttca tcagctgttt
     3961 ttttagcgag ctcaaggtag tgaaagttgg gtagaatagg atgaatattg gcacttttta
     4021 gtgagtaaaa gtacaatcaa aaatgaagta ataaattagg gtggaaccgc gtctttgacg
     4081 cccctgtgct gttgggcata ggtggtgtag gacgtggttt ttatcatttg tccgttttaa
     4141 gtcaattgtt gtcaccttcc caacagctgt aataaagtat gaaagaagga aaatgatgtc
     4201 taagaaatta acttttcaag aaattattct aactttacaa cagttttgga atgaccaagg
     4261 ctgtatgtta atgcaagctt atgataatga gaaaggagca gggacaatga gcccttatac
     4321 tttcttacgt gctataggcc ctgagccttg gaatgctgca tacgttgaac catcacgtcg
     4381 tccagctgat ggccgctatg gtgaaaatcc aaaccgtctt taccaacatc atcaatttca
     4441 agtggtaatg aagccttcgc cgtcaaatat tcaagaactt tatttgaagt ctttggaatt
     4501 actaggtatc aatccacttg agcatgatat ccgttttgtt gaagataact gggaaaatcc
     4561 atctacaggt tcagctggat taggttggga agtttggctt gatggaatgg aaataacaca
     4621 atttacttat ttccaacaag taggtggact tcaaacaggt cctgtaactt ctgaagttac
     4681 ttatggacta gagcgtttag cctcttatat tcaagaggta gattctgttt atgatattga
     4741 gtgggcacca ggtgttaaat atggtgaaat ctttactcag ccagagtatg agcattcaaa
     4801 atattcattt gagatatcag accaagtaat gttacttgaa aactttgaga aatttgaaag
     4861 agaagcaaaa cgtgctttag aagaaggact tgttcatcca gcttatgact acgtccttaa
     4921 atgttcacac acctttaatt tattagatgc acgtggagca gtatctgtta ctgagcgtgc
     4981 aggctatatt gcacgtattc ggaatttagc acgtgttgtt gctaaaacct ttgttgccga
     5041 acgtaaaaaa cttggtttcc cacttttgga tgaggaaact cgtatcaaat tacttgctga
     5101 ggaagactaa tgaacacact cattgttaat agtcatcctg atttttcgaa tccgtactca
     5161 tttacaacta ttctacaaga aaagtttatt gaactttata atgagcattt ccctaaccat
     5221 cagttatcta ttttaaattt atatgattgt gttttgccag aaattacgaa agaagtacta
     5281 ctttcgattt ggtctaaaca aagaaagggt ttggaattaa cagccgatga gattgttcaa
     5341 gctaaaatct ctaaagattt acttgaacag tttaagtcac atcaccgtat tgtctttgtt
     5401 tctccaatgc acaattacaa tgtgactgct agagcaaaaa cttatattga taatattttt
     5461 attgcaggtg agactttcaa gtacactgaa aatggttcgg tcggattaat gacagatgac
     5521 tatcgtctgc ttatgttaga atctgcgggt tctatttatt ctaaaggaca atatagtcca
     5581 tatgaatttc ctgttcatta tttaaaagct atctttaaag attttatggc ttttgatgat
     5641 ttctgtgttg ttcgtgcaga aggtaccgat attttagata ggcaagttgt tcttgataag
     5701 atgaatcagg atttaagaga agcatttgaa gcgttttata gtaaggagta ggaaatgact
     5761 aaagatttat tattggagct tggacttgaa gagttgccag cttatgttgt gacaccgagt
     5821 gagaagcaat tgggccaaaa aatggtcaag tttttagagg atcatcgttt atcatttgaa
     5881 actgttcaaa ctttttcaac tccacgtcgt ttggctgttc gtgttaaagg attggcagac
     5941 cagcagacag atttaacaga agatttcaag ggtccttcta agaaaattgc tttggatgca
     6001 gaagggaatt tttctaaagc agcccaaggt tttgttcgcg gaaaaggttt aagtgttgat
     6061 gatatcgaat ttcgtgaagt taagggagaa gaatatgttt atgtcactaa gcatgaaact
     6121 ggaaaaagtg cgattgatgt tttagcgagt gtgacagagg tattaacaga attgactttc
     6181 ccagtgaaca tgcattgggc aaataatagt tttgaatata ttcgtccagt tcacacttta
     6241 gtggtattat tagatgatca agccttagag ctagacttcc ttgatattca ttcagggcgt
     6301 attagtcgag gacaccgttt cttgggttct gacactgaaa tttcatctgc cagttcgtat
     6361 gaagatgact tgcgacaaca atttgttatt gctgatgcaa aagaacgtca gcaaatgatt
     6421 gttaatcaaa ttcatgctat cgaagaaaag aaaaatattt cagttgaaat tgacgaagat
     6481 ttactaaatg aagtgcttaa tttagttgag tatccgacag cattcttggg ttcatttgac
     6541 gagaaatacc ttgatgtacc tgaagaagtt ttagtcactt caatgaaaaa tcatcaacgc
     6601 tattttgttg ttcgcgacag ggatggaaaa ttgttaccaa actttatttc tgtgcgtaat
     6661 ggaaatgctg agcatatcga aaatgttata aaaggcaatg aaaaagtttt agtggctcgt
     6721 cttgaagatg gtgaattctt ctggcaagaa gatcagaaac ttaatattgc agatttagtt
     6781 gagaaattaa aacaagtaac tttccatgaa aaaattggtt cactttatga acatatggat
     6841 cgtgtaaaag ttatttcaca acatttggct gataaggcag gtctttctga tgaagaaaaa
     6901 ttagctgtgc ttcgagctgc aagtatttat aaatttgacc tcttaactgg aatggttgat
     6961 gagtttgatg aattacaagg tattatgggt gagaaatatg ccttacttgc aggggaacag
     7021 cccgcagtag cagcagcgat acgtgaacat tatatgccga catcggccga tggagagctt
     7081 ccagaaacca gaattggtgc aattctagcg ttagctgata aatttgacac cttactttca
     7141 ttcttctcag ttggtttgat tccaagtggt tcaaatgatc catatgctct tcgtcgagct
     7201 actcagggga ttgtccgtat tttggaagct tttggttggg atatcccact tgatgaatta
     7261 gtaactaacc tttatggtct atcgtttgct agtttagatt atgctaatca aaaagaggtc
     7321 atggccttta tttcagctcg tattgaaaag atgattggct ctaaggtccc taaagatatt
     7381 cgagaagccg ttttagaatc agacacttat attgttagcc ttattctaga agctagccaa
     7441 gcattggtgc aaaagagcaa ggatgctcaa tataaagttt ccgttgaaag tttgtcacgt
     7501 gcatttaatt tagctgaaaa agttactcat tctgtatcgg tggactcttc attgtttgaa
     7561 aataatcagg agaaggcgct atatcaagct attttgtcac ttgaactgac tgaggatatt
     7621 catgacaact tagataagct tttcgcactt agtccaatta ttaatgattt ctttgataat
     7681 acaatggtaa tgacagatga tgaaaaaatg aaacaaaatc gtctggcttt actcaatagc
     7741 cttgttgcaa aggcaagaac agtcgctgct tttaacttat taaacacaaa ataaggaggc
     7801 attttatgga tcctaaaaaa attgctcgta ttaacgagct ttcaaaaaag aaaaaaacag
     7861 ttggattgac cggagaagag aaggttgaac aagcaaagct ccgtgaagag tatattgaag
     7921 gtttccgccg atcagtccgt caccacgttg aaggaattaa attagtggat gatgagggca
     7981 atgatgttac cccggaaaaa cttcgtcaag tccagagaga aaaaggacta catggtcgta
     8041 gtttggatga tccaaattct taatggtgag acaattttaa attaaagtca acacttgggt
     8101 ggggcaaaag ataatctttg tctcgccttt tttattagga aggaaaaaat aatgattgaa
     8161 ttactattag cattactgtt atggaatatt ttagtatttg caacctatgc tattgataaa
     8221 aggcgtgcaa ttagaggggc atggcgtatt agtgagaaaa cattgttgat agagagttta
     8281 ttattagggg gatttggtgc attcctaggt ggagaagttt ttcgacataa aacgactaaa
     8341 tggtatttta gactagtttg gtatatcgga atgattattg atttagctat tatttggcta
     8401 atttggtgtt attgatgata tcttatcaaa aattttaggg gcatattaaa tttttgatag
     8461 tgcaggatag actgatttag gtataataga attacaaaaa gaaaacgctt acttttaagg
     8521 aggaagttta tgtcttcaga agaaaaatac gttatggcga ttgaccaggg aacaacaagt
     8581 tctcgagcaa ttatttttaa taaaaaaggt gaaaaaattg cgagtagcca aaaagaattt
     8641 ccccaaattt ttcctcaagc aggctgggta gagcataatg ctaatcagat ttggaattct
     8701 gttcaatctg tcattgctgg agcttttatt gaatcaagca tcaaacctgg acagatagag
     8761 gctattggta ttaccaatca acgtgaaaca actgttgttt gggataaaaa aacaggtctt
     8821 ccaatttata atgctattgt atggcaatcg cgacaaacag ccccgattgc tgatcaattg
     8881 aagcaagaag gtcatactaa tatgattcat gaaaaaactg gtcttgtcat tgatgcttac
     8941 ttctcggcaa caaaagttcg ttggatttta gaccatgttc ctggagcaca ggaacgtgct
     9001 gaaaaaggag agttgctttt tggaacaatt gatacttggt tagtttggaa gttaactgac
     9061 ggtctcgtcc atgttacaga ctattcaaat gctgctcgta cgatgcttta taatattaaa
     9121 gaacttaaat gggatgatga aattttagag ttactgaata ttcctaaagc aatgttacca
     9181 gaagtaaaat ctaattctga agtttatggg aaaactactc cattccattt ttatggtgga
     9241 gaggtgccaa tttctggtat ggctggtgac caacaggcag cattatttgg acagttggcc
     9301 tttgaacctg gtatggtaaa aaacacttat ggtacaggtt ccttcattat catgaatacg
     9361 ggcgaagaga tgcaactgtc tcaaaataat ctattaacaa cgattggtta cggcattaat
     9421 ggtaaggttc attatgcact tgaaggatca atcttcattg caggaagcgc tattcaatgg
     9481 ttacgtgatg gtcttagaat gattgaaacg tcaagtgagt cagaaggcct tgctcaatca
     9541 tcaacaagtg acgatgaggt ttatgtggtt ccagctttta caggtttggg tgcaccgtat
     9601 tgggattcaa atgctagagg gtcagtattt ggattaactc gtgggacaag taaagaagat
     9661 tttgttaaag ctacacttca gtcaattgct tatcaagtac gagacgttat tgacactatg
     9721 caagttgatt caggaattga tatccaacaa cttcgtgtag atggtggagc tgcaatgaat
     9781 aatttattaa tgcaattcca agcggatatt ttagggattg atattgctcg tgcgaagaac
     9841 cttgaaacta ctgccttggg tgcagctttc ttagccggac tttcagttgg ctattgggaa
     9901 agtatggatg aactaaaaga actgaacgca acaggacaac ttttccaagc aacaatgaat
     9961 gagtctcgaa aagaaaaact gtataaaggg tggagaaaag ctgtcaaagc gacacaagta
    10021 tttgctcaag aggattaagg gggtgtagag atggaatttt cacgagaaac acgacgttta
    10081 gccttacaaa gaatgcaaga tcgtactctg gatttactta tcataggtgg gggtatcaca
    10141 ggcgctggtg tcgctttaca agcagcagct agtggattag atactggact tattgaaatg
    10201 caagattttg cagaagggac aagtagtcgc tcaacaaaat tggtccatgg tggtcttcgt
    10261 tacttgaagc aatttgatgt agaagttgtt tctgatacag tatcagagcg tgcagtcgtg
    10321 cagcaaattg caccacacat tcctaagcca gatccaatgc ttttaccggt ttacgatgag
    10381 ccgggttcta cttttagtat gtttcgcctt aaagtggcta tggatttata tgatttatta
    10441 gcaggtgtga caaacacacc cgcagccaat aaagttttaa gcgcagaaga tgttcttaag
    10501 cgtgagcctg atttacaaaa agaaggtttg ctaggcggag gggtctattt agatttccgt
    10561 aataacgatg cacgcctagt gattgaaaat attaaacgtg ctaataggga tggagcttat
    10621 attgccagtc atgtcaaagc tgaggacttc ttatttgatg acaacaatca aattatagga
    10681 gtacgtgcgc gtgatttgtt gactgaccaa gttatcgata taaaagctag acttgttatt
    10741 aatacaactg gaccttggag tgacaccgtt cgcaacttct ccaatgaagg taaacaaatt
    10801 catcaattac gtccaactaa gggagttcac ttagttgtag atcgccaaaa attaaatgtt
    10861 tctcaacctg tttatgttga tacaggtcta aatgatggtc gcatgatttt tgttttgcca
    10921 cgtgaagata agacttattt tggaaccaca gatactgatt accatggaga cttagaacat
    10981 cctacggtaa ctaaagaaga tgttgactac ctccttaata ttgtaaacaa acgcttccca
    11041 gaagcagagc ttactattga tgatattgag agttcatggg caggtctgcg tccactacta
    11101 tcagggaata gtgcctctga ctataatgga ggcaatagtg gaaaactaag cgatgaaagt
    11161 tttgaagaat taattgattc cgttaaagat tatattgcac ataaaaatca tcgtgaggat
    11221 gttgaaaaag ctatttctca tgtagaatct agtacttctg aaaaagaatt ggacccatca
    11281 gctgtttctc gaggatcaag ttttgagcgt gatgataatg gcctattaac acttgctggt
    11341 ggtaaaatta ctgattatcg aaaaatggca gaaggtgcca tggaaactat tattaacatt
    11401 ttggataaag aatataatcg caagtttaaa ctaatcaatt ctaaaaccta tcctgtttca
    11461 ggaggagaga ttaacccatc taatgttgat tctgaaattg aagcttatgc tcaattgggc
    11521 acactaagtg gtttaagtat tgaggatgca cgttatattg ctaaccttta tgggtcaaat
    11581 gcaccaaaat tatttgcatt gacacgccaa attacagaag cagaaggtct gagcttggta
    11641 gaaacgttgt cacttcacta tgctatggat tatgaaatgg cgcttagccc aacggatttc
    11701 ttcttaagac gtacgaatca tatgcttttc atgagagata atttagattc tttgattcag
    11761 cctgttattg atgagatggc taaacattat caatggtctg atcaagacaa aactttctat
    11821 gaagaagaat tacatgagac tctaaaagac aatgacttgg cagctttgaa ggataattga
    11881 aggagataat aatggatatt tttggagaat tcttaggaac agctctctta gtcttacttg
    11941 gtaatggtgt tgtagcaggt gttgttttgc ctaaaacgaa gaatcataat tctggatgga
    12001 ttgttatcac atttggatgg gggcttgctg tagctattgc agcgttggta tctggcaata
    12061 ttagccctgc ccacttgaat ccagctgtgt cattagcttt tgcgatcaaa ggtgatttag
    12121 cttggggaac agctattctt tatatgattg cacagattat tggtgcaatg ctaggttcac
    12181 ttcttgttta tttgcaattt agaccccatt atgaggctgc agaaaataaa gcagatatcc
    12241 ttggaacttt tgcaactggt ccggctttga aggataattt ttccaatttt ttaagtgaag
    12301 tattgggaac acttgttctt gtacttacta tttttgccat cggaaaatac aatatgcctc
    12361 caggagttgg tacgatgagt gttggtatgt tagtggttgg gattggtcta tcgctcggtg
    12421 gaacaacagg ttatgctatt aatcctgcac gtgattttgg tcctcgttta ttgcatgcct
    12481 tgttaccgat gaagaacaaa ggtgattctg attggactta ttcatggatt ccgatagtag
    12541 gacctatggt aggagctatt ttagcagcct taatatttgc tatgatgtaa gagatttttg
    12601 atgctgagac cgactttatg gtctcagttt tttgttaccg ttttcaaaaa taacttcttt
    12661 tgttataata agtaattatg acagaaaaat atgtaaataa agtaattgtc attttaggag
    12721 cttcttttgc tggtatgact tgtgcgcaga aattacgcca attaaatcct aattgggata
    12781 tcgttttgat tgataaagaa attcatccag attatgttcc aaatggctta aattggtatt
    12841 atcgtcacga aattagtggt ttaaatcagg ccatgtggca gacagaagaa gagcaacggt
    12901 tgcagaatat acgctgtcta tttggtctaa aagttgagaa aattaataaa gaagataggg
    12961 aattaatgtt atcagatggt tcttctgtct attatgatca attgatttgt gccatgggct
    13021 cccaagcaga atcaacttat attgatgggg ctgatgcgca aggtgtttta acaacaaaaa
    13081 cttatgcaac tagtcaaaat gctaagcagg tattagataa aagtcataag gtagcagttg
    13141 ttggggcagg tatcatcggt ttagatattg cttatagttt acatgaatca gggaaggcgg
    13201 tcactttgtt agaagctcaa gaaagaccag acttcaggca tacagatcct gatatgtccc
    13261 ttcctttact tgatgcgatg gctgaatcaa aactacattt tttccaaaat caaaaggttg
    13321 agaaaattac tgtaacccga gaagaaaaac tttgtttgcg aacgctaaca ggagacacct
    13381 ttacagttga tactgtcata ttagctgtta attttcgtcc ggatagtcgt cttttaactg
    13441 ggttggttga tttaagtgtt gataatagtg ttgttgtaaa tgattatttc caaacgagtg
    13501 accctaatat ctatgctatt ggcgatttga tttggtcgta ttttaaaggt cttaatagtg
    13561 cttattatat gcccttaatt aaccaagcaa tccgttcggc acaaatgttg gcataccatt
    13621 tatcgggaca tgctgttcca aaattaaaaa taacacgcgc tacaggatct aaacactttg
    13681 gctattatag agctaacatt ggattaactg agctggaagc tggtctttac gaggataccg
    13741 ttagtgttac ttatttccct aaagaacagt atgatttaag gataaaattg attgctaatc
    13801 aaaaaacggg ccatttattg ggggctcaat taatctcaaa ggaaaattgt ttggctactg
    13861 ccaatcagct tgttcaagct atatcgtgtg acatgacaga ttttgatttg gcttttcaag
    13921 atttcattta tacagctaga gaatctgaga tggcttatat gttacatcaa gcagctatca
    13981 acctttatga aaagaggatt gggctatgcg aatagaagct ctaatggaaa aagaaaggcg
    14041 tgttcaatat cggctacttt cttttttaag aggctctcct caggcaatag cactgaagtt
    14101 agctttatta gaaactggtt taagtcgagc aactttgctt aaatatatca ataatcttaa
    14161 tagctacttt gaacaagaaa aagttaattg tcgaatcgtt tattataagg ataagttatt
    14221 tttagaagag gattataact tatccaatca agaagtgttg aaagctctga tgaaagatag
    14281 tattaaatac actatcttaa tatctttatt taatcagcgt cagttcacaa tcgttggtct
    14341 ttctcaagaa ttgatggtta gtgaggctac tttaaatcgt catcttgctc atctcaatga
    14401 attgttagcg gaatttgata ttgctatttc acaaggaaaa cagattggtg atgaattgca
    14461 atggcgttat ttttattatg aattattcaa gcaactatgg tcttatgaca agtgccaaaa
    14521 tatgataaag aaattagact tagactcatt aattctatta atagagcgct tggctcaaca
    14581 tactttaact cgagaagctc agcaaaatct ggggctttgg tttagtattt gtcatcatcg
    14641 tctccttgct atggaaaaac tatctgataa tctcaagccc attgtggaac attatcaatg
    14701 taatgctttt tataaacggt tagacgcagc cttagttttg tatatgagtc gttttgctct
    14761 tgagtatagg gaaggagaag ttctggcaac ttttgctttt ctacattcac aaaatatttt
    14821 accaatcaat acaatggaat acatcatggg atttggaggc ccgattattg attgtgtaac
    14881 cgaaacaatc atctacttta aaaaggagag tattttagct gacgagacat cagatcaggt
    14941 tatttaccaa cttggtcagt tatatagcca ttattatttc tttaaaggtc atattttagt
    15001 tgaacaacct gatttagaac aaacgtatcg acttattgat cataatatga gggataagtt
    15061 gcatgatatt tcaaagaaga ttattgcaaa tgttaatcga attagacctt taacagaaga
    15121 tggttgttct ttgctgactt tacatttgtt ggaattgctt attttctcga aaaatagtca
    15181 aaaaatgccc tttaggattg gcctagatat gacaggtaat gctgtggaac agtcattgtt
    15241 ggaatatcgc attcgacaac atttctctgg taataattct attcaagtcg aaccttatga
    15301 cgaaggaaag ggatttgata tggttattta tcagagtcgc tcaagacctt ataaagccaa
    15361 actaacctat tgccttaata aaggtgcttc agagcgagaa ctacaggaaa tagattcact
    15421 tatttatgac aattaatccc attacttaat tgaatggttt taattcctcc agaaattgtt
    15481 aaaacgttta cgtattttat gttataatat caaagacgaa caattttaaa tttagttgtt
    15541 aaaaacagaa aggttttatc atgacatttg acaccattga tcaattagcg gttaatacag
    15601 tccgcacgct ttctattgat gctatccaag cagcaaattc tgggcaccca ggtcttccta
    15661 tgggagctgc gcctatggct tatgtgcttt ggaataaatt cttaaatgta aacccaaaaa
    15721 caagtcgcaa ttggacaaac cgtgaccgtt ttgtactttc agctgggcat ggttcagctc
    15781 ttctttatag cctacttcat ttagctggct atgatttatc aattgatgat ttaaaacaat
    15841 tccgccaatg gggatcaaaa acacctggac atccagaagt taaccatact gatggtgtag
    15901 aagcaacaac tggaccttta gggcaaggta ttgctaatgc tgttggtatg gcaatggctg
    15961 aagctcattt ggctgctaaa tttaataaac caggctttga tttagttgat cattacactt
    16021 atacacttca tggtgatggt tgtttgatgg agggtgttag ccaagaagct gccagtcttg
    16081 ctggacactt aaaacttggt aaattggtcc ttctttatga ttcaaatgat atctcacttg
    16141 atggtccaac gtctcaatca tttacagaag atgttaaagg gcgttttgaa tcatatggtt
    16201 ggcaacatat cttagttaaa gatggtaatg atttagaagc gattgctgca gcaattgaag
    16261 ctgctaaagc tgaaactgat aaaccaacca ttattgaagt taaaactatt attggatttg
    16321 gcgcagagaa acagggaaca tcttcagttc atggagcgcc acttggtgca gaaggtatta
    16381 cttttgccaa gaaggcttat ggatgggaat atccagactt tactgttcct gctgaagttg
    16441 cagctcgatt tgcctcagat cttcaagcgc gtggtgcgaa ggcagaagag gcatggaatg
    16501 atctttttgc taaatatgaa gttgagtatc cagaattagc agctgaatac aaagaagctt
    16561 ttgctggcca agctgagaca gttgaattga aagctcatga cctaggttca tcagtggcta
    16621 gtcgtgtttc aagccaacaa gctattcaac aattatcgac tcaattaccg aacctctggg
    16681 gagggtcagc agacctctca gcttctaata acacaatggt agcagcagaa acagatttcc
    16741 aagcaagtaa ctacgctggg cgtaacattt ggtttggtgt acgtgaattt gcaatggctg
    16801 ctgcaatgaa tggaattgcg cttcatggtg ggactcgtgt ttatgggggt acattctttg
    16861 tcttctcaaa ctacttgtta ccagcagtac gtatggctgc acttcaaaac ttgccaactg
    16921 tctatgttat gacacatgat tctattgctg ttggagaaga tgggccaact catgagccaa
    16981 tagaacaatt agcatcagtg cgctcaatgc ctaatttgaa cgttatccgt cccgcagacg
    17041 gtaatgaaac aaacgctgca tggcaacgag ctgtatcaga gacagatcga ccaacgatgc
    17101 ttgttttaac acgtcaaaac ttaccagttc ttgaaggaac atcagaatta gcccaagaag
    17161 gggttaataa aggtgcttat attttatcag aagctaaagg tgaacttgat ggtatcatca
    17221 ttgcaacagg ttcagaagtt aaattagctt tagatactca agacaaactt gagtcagagg
    17281 gcatccatgt tcgcgtcgta tctatgccag cacaaaacat ttttgatgaa caagaggctt
    17341 catatcaaga gcaagtgctt ccatcagctg ttacaaaacg tcttgctatt gaggcaggat
    17401 caagcttcgg ttggggtaaa tatgttggtt taaatgggct aacacttact attgatactt
    17461 ggggagcatc agcaccaggt aacagaattt tcgaagagta tggctttaca gttgagaatg
    17521 ctgtatcgct atataaagaa ctttaagagc atgaagaagt tgggaaaaat acctcaactt
    17581 ctttttgtaa gatataggat ggatttgaga atagttttga tagactgata agtgatggaa
    17641 agcactcttt tttgaaaacg tttgacaaac attccatagc gcagtataat tgataaaact
    17701 attgtatata taaaataata caactattat taattatctt tttagaaagg tttgtttatg
    17761 aagaaggagt gtcgtgattt ttatcggcag atacagcata cttacaatga tatatctgtc
    17821 cgtgaagatg cagtgctatc atcaatacta ttatcagcat cgaatggttt gataaaaact
    17881 agcgatgtac ctcgtgttgc ttatgagtta actcagcaat tggaaaataa tgagatagaa
    17941 aaatctttcg agtcattagc tacagttaag gaattaaaaa aatctgctaa aaaattgtct
    18001 gagagatatg agcaaaataa ggtaacaacg ggattgcttt ctaaagtatt tggaaatcat
    18061 taggagataa aagaagattt ttatgattaa gtttgaacat gtttcgaaag tatacgggga
    18121 aaaagaagct ctcagtgatt taaccctctc cattaaagat ggtgagattt ttggattaat
    18181 cggtcacaat ggagcaggaa aaacaacgac aattagtatt ctaacatcta ttattgatgc
    18241 aacttatggc caagtctata ttgatgacct acttttgaca gaacatcgtg accaaattaa
    18301 gaaaaaaata ggttatgtac cggactcgcc agatattttc ttgaatttaa cagcagagga
    18361 atactggtac tttttagcta aaatttatga tgttgcccca gaagatattg aagcacgtat
    18421 cacaaaattg gtagacatat ttgaactaga ggagcagcgc tataatccta ttgaaagttt
    18481 ttcacatggt atgcgtcaaa aagtcattgt tataggtgcc cttcttccaa atcctgacat
    18541 ttggattcta gatgagccat taactggttt agatcctcaa gcttcttttg atttgaaaga
    18601 aatgatgaaa gaacatgcta aaaatggaaa aacagttatt ttttcaacac atgtcttagc
    18661 agttgcagaa cagttatgcg acagaatagg tattttgaaa caagggaaat taatctttgt
    18721 cggtagctta ggtgagttaa aaatgcaata tccagataaa gatttggaaa ctatctattt
    18781 ggaattagca ggacgtcaag ctaataggga gggataaagt atgaattggt cacgtatctg
    18841 ggaactcgta aaaattaata tcctttattc aaaccctcag actctatcgg cactaagaaa
    18901 aaagcaagaa aagcatccta aaaaagaatt ttcagcttat aaatccatgt ttagaaatca
    18961 gttatttcag attttgctct tttcaataat ttatgtattt ctctttgtat cacttgattt
    19021 taaagaatat ccgggctatt tcacgttcta cattggtatc tttacactag tatccattat
    19081 ctactctttt attgcgatgt acagtgtttt ctatgagagt gacgatgtta aacaatatgc
    19141 ttatttgcct attaaatctg aagagcttta tgttgctaaa atttttgcaa catttgggat
    19201 gtctgtaact tttctaatgc caatcctaac cttaatgatt gtggcttatt ggcgtataat
    19261 aggtggccca cttgcggtcc tcctagcaat cataaatttt gctattttat ttttatcggt
    19321 aacggtgatt tcattgtata ttaatagttt aataggccgt gccattattc gaagtgctaa
    19381 tcggaaactt atttctacta tattaatttc actagctaca tttggagcga tagttccctt
    19441 attatttgtg aatatgacca gtcaaaagat ggtacaaggg aaattacaag atatcgctcc
    19501 aatttcttat gtcagaggtt attatgatat tgttacagcg ccgtttagta tggagagtct
    19561 tctcaactat tatctcccgc tcttaatcat tttattcttg ataggtgcta tttataaatg
    19621 ggttatgcca cgctactatc aggagttgtt gtatggacag gtcaagcaga gaaaagtcca
    19681 tcgtcaaatc gattttagta aacgtgagtc aattaataaa actcttgtta aacaccattt
    19741 atcaagttta caaaatgcga cattattgac aaatacattt ttaatgccac tattatatct
    19801 agcaatgttc attgttccaa ttttgaataa tggtaaagag attggtcgat tttttaatga
    19861 aaattatttt gggatagctt ttctagcagg aatattaata ggtagtttat gtgtgatgcc
    19921 agctagcatt gtaggggtag gtatttcttt agaaaaaagt aatttctact ttataaaaag
    19981 tttgcctata tcattttcgt atttcttaaa gcataaattt gtaactttga taacattaca
    20041 acttgcagtt cctactttta tttatttctt ggtaggattc tttctactta aactatcaat
    20101 cctcgtttta ttgagcttca ttttaggtct agtcttcatg ggacttatcg aaggccagtt
    20161 tatttataga cgtgactata aacatttgtt cttgaattgg caagaggtta cgcaactatt
    20221 taatagaggg ttaggccaat ggctgttagt tggttcattg tttggaatga tgataatagg
    20281 tagttttctt attggaataa gtattttttg gtcaatggta tggaatactg tagctgtcaa
    20341 tatcataatc cttatcattg ggttgctcat attatcaatt tgccaatact tactgctaaa
    20401 aaatttttgg aagaagcttg tttagtaaaa agattttaga aaaatcatac agtaagaata
    20461 atgcttaata actgagatag ttatagcgga taaaataaag gcttgttgtg gactttacgg
    20521 ttggaaggca gccactgtaa tcctttgttt aataacgcta gttttagctc taaaatattt
    20581 gatttttatt gctatctatt atatcttatc agacaaagag actagggata acctagtctc
    20641 tttgtctgat gctaacttgt ttttcgtaat attgtgctag aatgaatgtc aatatagcag
    20701 ttgtgcttac agtaatgata actgcaataa gtatggtaat aaaaagatgt gttgaagtat
    20761 gactgatata aagaaataga cctggtagac cagaagagcc tacagcgaag cggtgagcgt
    20821 tcattatacc ggcatagagt cctccaagac caccaccaat aagtgaggct attagaggat
    20881 aacgtttcgg aaggttgaca ccatataaga taggttcggt gattcccata taagctgtta
    20941 tggcagcagg tccagcaatt tctttaagct ttttttcttt tgttataaga gtcaccactg
    21001 tacctgcagt tgcttgtgcg atatttgagc agagcatgcc tggtccaaaa atagaatcaa
    21061 cccctaattt cgctagttgt gtgattccta atgcagcaat tccactatga acaccaaaca
    21121 tgattaggat aggagcaaat gctcctacta gaaaagcagg tgcccaactg gcgtatttgg
    21181 ataaaaaaag taaaaataac agctaaatat tggccagcga tagtacccat tggtcccaat
    21241 atagagaacg acaaaattcc catgctgaga aatattaaca taggaacaag cactaaacgt
    21301 agtgatttag gaataatttg atttaaatat ttttctatat aagcctgaac acaaataatt
    21361 agaataatgg gaatgacact tgatgcatag ttagtaagtg tgaatgggat cgtatggaat
    21421 aaggatactg gtttaccaga agctactaaa ttagcccaat tagggtgaag gagcatgaca
    21481 acagttccca aagctaaaat aggattagcc ttcaattttt gagcagctgt gatggcgatt
    21541 aagataggta aaaaatagaa aacaccatct gcaaaaagat ttagtaatat atatgtttga
    21601 gataacggtg tcaagatttt gaatactagt aataaagcta gcaatgcctt aatcatacca
    21661 gcgcctgata gagctggtaa aatcggttgg aaagtcccag aaacaaaatc aataattcga
    21721 ctaactgctt tggctttttt ggtttgttct gttttagtcg ttgagttagt ttcaatgagg
    21781 gagttaattt cttcaaacac ttttgcgaca tgagtcccga tcactatctg atgttgccct
    21841 ccgctgataa cgacagtttt tacctctttt aaagttttta attgctcaag gtttactttc
    21901 gtcggatcat tgagactaaa gcgaaggcgt gtttgacaat ggtaaacatt gttaatatta
    21961 ttttgcccac caaggtgttt gatgatattt tttgacaagg tgatgtattc tctagacata
    22021 atatctcctt aatacttata aaacgttttc cgattctaga ttatagcata gtaataaaag
    22081 aaaacacttg gaaatgcttg tgaattttca gtgatgtttt cagatagcat tcctaaaggg
    22141 tctatgatat aattttttta tgaaaagaca ttttgagaca actagaagaa ttgttattaa
    22201 agtaggaacc agttccctcg tacagacaag tgggaaaatt aatttaagta aaatagacca
    22261 tttagcattt gttatttcta gtcttatgaa taaaggtatg gaggtgattt tagtatcatc
    22321 tggagctatg gggtttggtt tagacatttt gaaaatggat aagcgtcctc aagaaatatc
    22381 acaacaacaa gcagtttcta gcgtaggaca agtagccatg atgagtcttt attctcagat
    22441 attttcgcac tatcaaactc atgtttccca aattttatta acacgtgacg ttgtggtttt
    22501 tccagagagt ttgcaaaatg taacaaactc ttttgagagt ctgctctcta tgggaatttt
    22561 gccaatcgtc aatgagaatg atgctgtcag cgtggatgag atggatcata aaacgaagtt
    22621 tggggacaat gaccgtctct ctgctgtcgt tgctaaaatt actaaagcag atttattaat
    22681 tatgctgtca gatattgatg gtctttttga taaaaatcct aatatttatg atgatgctgt
    22741 tttacgtagt catgtttctg agatcaccga tgacatcatt aagtcagctg gaggagcagg
    22801 aagtaagttt ggtactggtg gcatgcttag taaaattaaa agtgcacaaa tggtgtttga
    22861 taacaatgga caaatgatat tgatgaatgg cgccaatcct cgtgatattt taaaggtgtt
    22921 agatgggcat aatattggaa cttattttgc tcaaggaaag tgagattgag ttatgacgta
    22981 tattgaaata ttagggcaaa atgctaaaaa agctagtcaa tcagtagcac gattaagtac
    23041 agctagtaaa aatgagatac tcagggactt agcgagaaac atagtggctg atactgaaac
    23101 cattttgact gagaatgctc gcgatgttgc taaggcaaaa gataatggta tctcagaaat
    23161 tatggtggac cgtctacgac taaacaaaga ccgcatacag gcgatagcaa acggtattta
    23221 tcaggtagca gatttggcag atccgattgg tcaagttgtt agtggttata caaatttaga
    23281 tggtttaaaa atccttaaaa aaagagtgcc tttaggggtt attgcaatga tatttgaaag
    23341 tcgtcccaat gtatcggtgg atgcctttag tttagctttt aaaacaggga acgctattat
    23401 tttacgtggt ggtaaagatg ctattttttc aaatactgcc ttggttaatt gtatgcgcca
    23461 aacattgcaa gatacaggcc acaacccaga tatagtacaa ttagttgaag atactagtca
    23521 tgttgtagca gaggagttaa tgcaggcaac agactacgtg gacgtcttaa ttccaagagg
    23581 tggtacaaaa ttaattcaaa cggtgaagga aaagtcaaag attcctgtta tcgaaacagg
    23641 tgttggaaat gttcatatct atattgatga atttgcagat ttagatatgg cagctaagat
    23701 tgttattaat gctaaaaccc aacgccctag tgtctgtaat gctgctgaag ggttagttgt
    23761 tcatcaagct attgctaagg gatttttatc tcaattggag aaaatgctca aagaatcgaa
    23821 ccagtcagtt gaatttcgtg ccgatgagga agctttacaa cttttagaaa atgctgttgc
    23881 tgcaagtgag actgattatg ctactgaatt tttagactat attatgtcag taaaagtagt
    23941 agattctttt gaacaagcaa ttagctggat taataaatac agtagtcatc actcagaagc
    24001 tatcattact aataatatca gtagggcaga aatttttcag gatatggtag atgcagcggc
    24061 agtttatgtt aatgcttcaa cacgttttac agatggtttc gtttttggac tcggagcaga
    24121 gattggtatt tcgactcaaa aattacacgc gcgtggaccg atgggattag aagctttaac
    24181 aagtacaaaa tactatatta atggaactgg tcaagtaagg gaatagttga cctataatag
    24241 gagagattgc atttatcggt ctctctttta cttttctaaa gtttaggatt tatggtacaa
    24301 ttaaagttat gacaaacgat tttcatcata ttactgtgct tttgcacgag actgtggata
    24361 tgcttgatat taagccggat ggtacctatg tagatgctac tttaggtggg gcaggacaca
    24421 gcgaatatct tctttcacaa ttaggcccag atgggcacct ttacgcattt gaccaagacc
    24481 agaaggctat tgacattgct catatacgtt taaagaaata tgtggatacc ggtcaagtaa
    24541 cctttatcaa agataatttc agaaatctat cttctaactt aaaagcatta ggagtgtcgg
    24601 aaattaatgg tatttgttat gatttaggtg tatcaagccc acagctggac gagcgtgaac
    24661 gcggtttctc atataaacaa gatgcccccc ttgatatgcg aatgaataga gagcagagcc
    24721 ttacagctta tgacgttgtg aacacttata gttatcatga tttagtaagg attttcttta
    24781 agtatggcga agataaattt tcaaaacaaa ttgctcgaaa aattgagcaa gtacgagctg
    24841 aaaaaccaat atctacaaca acagaattgg ctgagattat caaatcgtct aaatctgcca
    24901 aagaactgaa gaaaaaaggt cacccagcta aacaaatttt tcaagctatt cgtattgaag
    24961 tcaatgacga actaggagct gcagatgaat ctatacaaca agcaatggac ttattagctg
    25021 ttgatggtcg tatatctgtc atcacttttc attctttaga agatcgtctt actaagcaac
    25081 tttttaaaga agcatcaaca gtagaagtac ctaaaggtct tccttttata ccagatgatt
    25141 tacaacctaa gatggagctc gttaatcgta aacccatctt accaagtcaa gaagaactag
    25201 aagcgaacaa tcgtgcccat tcagcgaaat tgagagtagc gcgtcgtatt cgataataag
    25261 aaagaaactt atgacaaatg aaaaacgaac tgaagcagta acacaaacct tacaacgtca
    25321 catcaagact ttctctcgca ttgagaaagc tttttatggc gctattgtaa ttactgctat
    25381 tatcatggca gtcggtatta tttacttgca aagtaatagt ttacaagtta aacaagaagt
    25441 aaatcaatta aatagtaaaa tcaacgataa acagacagaa tttgacaatg ctaaacaaga
    25501 agtaaatgag ctttctaatc gtgatcgaat tactaaaatt gctaaagatg ctggtttaac
    25561 aattcaaaac gacaatattt atcggaaggt ggactagtct gtgacttttt ttaaaaagct
    25621 aaaaaaaatc ttccttgatt atgtgatcca tataagagat aggcgttcgc cgcagaaaaa
    25681 tagggaaaga gtaggtcaaa atctgatgat tttaaccatt ttcctatttt tcatttttat
    25741 catcaacttt gtcatcatcg ttggtacaga tagtaaattt ggggttaatt tatccaagga
    25801 agccaagaaa gtctatcaac aaagtatgac agtacaggcg aagcgtggga caatttacga
    25861 ccgtaatgga aatcctatcg ctgaggatgc tacgacctat agtctctacg ctattatctc
    25921 aaagaactat acgacagcta caggtcaaaa actatatgta caaccttctc agtatgaaaa
    25981 agtagctagt atcttagaga ataaattggg catgaagaaa aatcttgtcc taaaacaact
    26041 caatcaaaag aaattgtttc aagtttcctt tggaagttca ggttcaggat tgtcttatac
    26101 caagatggct gacatcaaga aaacgatgga aaagtcagat atcaaaggaa ttggtttttc
    26161 taccagtccg gggcgtattt atccaaatgg gatatttgct tcacaattta taggatttac
    26221 tcttcctcag gatgatggag atggaaaaaa attagttggt aatacaggcc ttgaagcggc
    26281 acttaacaag gtattatcag gtactgatgg caaagtaaca tacgaaaaag accgtagtgg
    26341 gaatgttctt ttaggtactg caacaacaga acgtagggcg gttaatggta aggatatcta
    26401 cacaacttta tcagagccta ttcagactgt tttagagact cagatggatg ttttcgcaga
    26461 aaaaaccaaa ggaaaatttg cgagtgctac agttgtcaac gctaaaacgg gagaaattct
    26521 agcaacgagt cagcgtccta cttataatcc ttctaccctt aaaggttacg ataagaaaaa
    26581 tttgggtacc tataacacac tactatatga taattttttt gaacctggtt caacaatgaa
    26641 ggtaatgact ctagcctctg caattgattc taagcatttt aactctactg aagtgtataa
    26701 cagtgcacaa tataaaattg cagatgcggt tattcgagat tgggatgtca atgaagggtt
    26761 atcatctggt agttatatga cttttccaca agggtttgca cattccagta acgttggtat
    26821 ggtaaccttg gaacaaaaaa tgggacgaga taaatggtta aactatcttt ctaagtttaa
    26881 atttggttac ccaacacgtt ttgggatgct tcatgaaagt ggaggtttgt tcccatctga
    26941 caatgaagta actatcgcta tgagctcatt tggtcaaggt attggtgtaa cgcaagttca
    27001 aatgttgcgc gcatttacgt ctatctcgaa tgatggtgtt atgttacaac cacagtttat
    27061 tagcagtatt tatgatccaa atacagggac ttctcgtaca gcacgaaaag aagtcgttgg
    27121 aaaaccagtt tccaaagaag cagctagtaa aacacgtgat tatatggtta ctgttgggac
    27181 agatccttat tatggtacat tatacgcagc aggtgcccca gttattcagg tggggaatca
    27241 atcagttgca gtaaaatctg gtactgccca aatcgcccag gaaggtggtg gtggttatct
    27301 gcaaggaaaa aatgatacta ttaactcagt tgtggctatg gtgccatcgg aaaatcctga
    27361 cttcatcatg tatgttacca tccaacaacc cgaaaaattt agtatcacat tctggaaaga
    27421 tgttgttaat cctgttttag aacaagcaac agcaatgaaa gaaacgatat taaaaccggt
    27481 attgaatgac tcagaacatc agacaaaata taaactatca aaaatagttg gtgaaaatcc
    27541 aggtcatgta gcggaagaat tacgtcgtaa tctggttcag ccaattattt taggaaatgg
    27601 tagtaaagtt tctaaagtat ctaaaagacc aggagctaat cttgctgaga atgaacagct
    27661 actagtatta acaaataaac tgactgaatt acctgatatg tatggttggt ctaaagcaaa
    27721 cgttgaacag tttgctaaat ggacaggtat aaaagtgacc tataaaggtt caacctcagg
    27781 taaagtaaga aaacaaagta ttgatgttgg aaaatccatc aataaaatta aaaaaatcaa
    27841 aattacaata ggagattaac atgtttttaa gtataatggc aggtgtcata gcatttgtcc
    27901 tgacagttat tgccattcca cgcttcatta agttttacca attgaagaaa attggcgggc
    27961 aacaaatgca tgaagatgtc aaacaacatc tagccaaagc aggtacgccg acaatgggag
    28021 gaacggtatt tttggttgtt gctcttcttg tctcacttat ttttagtatt attttatcta
    28081 aagaaaatag tggtaattta ggtgcaactt ttggtatttt atctgttgtt ctcatctatg
    28141 gaattattgg ttttttagat gattttttga aaatttttaa acaaatcaat gagggcttga
    28201 ctccaaagca aaaaatgtcg ctccaactaa ttgcgggtct aattttttat tttgtccatg
    28261 tgttaccaag tggcacatct gcgataaata tttttggttt taaccttgaa gtaggatact
    28321 tatacgcttt ttttgttctt ttctgggttg ttggtttttc aaatgctgtg aacctaacag
    28381 atggaattga tggtctagct tctatttcgg ttgttatcag tttgattact tatggaatca
    28441 ttgcttataa tcaaacgcaa tttgatattt tattgattat cgttatcatg attggtgctt
    28501 tacttggctt ttttgtcttt aatcataaac cagctaaagt ctttatggga gatgttggtt
    28561 cacttgcatt gggtgctatg ttagcagcca tttcaattgc cttacgtcag gaatggacct
    28621 tgctttttat cggctttgtc tatgtttttg agacgtcttc agtgatgttg caagtagcat
    28681 actttaagta tactaagaag aaaactggtg ttgggaaacg tatcttccgc atgacaccat
    28741 ttcatcacca tttagaatta ggtggtgtgt ctggcaaagg taataaatgg agcgaatgga
    28801 aagtagatgc ttttttatgg gctattggta ttttcatgag cgctattaca cttgctattt
    28861 tatatcttta aactttatta ggggatgtgg tatggactgc cagattccct tttgctttct
    28921 tgtagccttt aatggtataa tgaacataat aatagtaagg aaatagacat gtcatttaaa
    28981 gattttaatt ttaaaccata cattcagagg gctctcgatg agttaaaatt cgtcgatcca
    29041 actgatgttc aagctaaatt gattccagtt gttcgttcag gccgcgatct tgtgggggaa
    29101 tcgaaaactg gttctggtaa aacccacacc tttttacttc ctatttttga gaagctagat
    29161 gaaagtagtg atgatgttca agttgttatt actgcaccaa gtagagagtt ggcaacacaa
    29221 atttatcagg caactaaaca aattgctgag cattcagagc aagaaatacg ggtggttaac
    29281 tatgttggtg gtactgacaa attacgtcaa attgaaaagt tgaaagtaag ccaacctcat
    29341 attgtcattg gaaccccagg acgtatttat gatttggtga agtcaggtga tttagttatt
    29401 cataaggcgc atacttttgt ggttgatgaa gcagatatga ctctggatat gggattcctt
    29461 gataccgttg ataagattgc agggtcttta cccaaggatg ttcaaatttt agtcttttca
    29521 gcaacgattc cacaaaaatt acagccattt ttaaagaaat atttgaccaa tccagtaatg
    29581 gaaaaaatca aaacagcaac agttatcgct gataccatcg ataactggct tttgtccact
    29641 aaaggacgtg ataaaaatgc acaaattttg gaactctcta aactaatgca gccttacctt
    29701 gcaatgatat ttgttaatac gaaagagcgt gcagacgaat tacactcgta tctttctagt
    29761 aatggcttaa aagtagctaa aattcatggt gggattgcac ctcgtgaacg taaacgtatt
    29821 atgaatcagg ttaaaaacct cgaatttgaa tatatcgttg caacagattt agcagcgcgt
    29881 ggtattgata ttgaaggtgt tagccatgtt atcaatgatg ccatccctca agacctatct
    29941 ttctttgtcc accgtgtcgg tcgcacaggc cgtaatggct tatctggtac tgctataact
    30001 ttatatcagc caagtgacga ttctgatatt cgtgaacttg aaaaactagg tattaatttt
    30061 attcctaaag tcattaaaaa cggtgaattt caagatacat acgaccgtga tcgtcgtaat
    30121 aaccgtgaaa agtcctatca aaaattagat acagaaatga ttggacttgt gaaaaagaaa
    30181 aagaaaaaaa taaaaccagg ttacaagaaa aaaattcaat ggaaagttga agaaaagcgt
    30241 cgcaaagagc gtagagcaag taatcgtgct aaggggcgtg cggagcgcaa agctaaaaaa
    30301 caaagttttt aaaattatta aagtctgaaa tcagttcgtt tcaggctttt ttattgatat
    30361 aaaatcttta tcacctgata aggaaatact attatacaga tagattttag agtgttagac
    30421 tagtaatatg atattttagg agaattctta tgtttaaaaa aataatatta tctattgcaa
    30481 ctatagccgc aaccgccagc ctagcagtat cagttcaggc gtcagagaaa gtagaactta
    30541 aagtagctac agattctgac acggcaccat ttacttatca aaaagacggg aaattcaaag
    30601 gttatgacgt tgatgttgtc aaagctgttt ttaaaggtag taagtacaaa gtaaccttca
    30661 agacagttcc ttttgatact atttcaacag gtattgatgc agggaaattt gatttatcag
    30721 ctaatgattt ttcatataat aaagaaagag cagaaaaata tctcttctca gatcctatat
    30781 cccgttcaaa ttatgccgta gtagggaaga aggggagcca ttacaaatca ttaagtgacc
    30841 tctctggaaa atcaacagaa gttttatctg gcgttaacta tgcacaggtt ctagaaaatt
    30901 ggaataaaaa tcatcctaat aaaaaaccaa taaaaatcaa atatgtttct gggacaactg
    30961 gtgttactag cagattaaaa aatattgaga gtggaaaaat tgactttatc ctatatgatg
    31021 ccatttcatc tgactatatt gtaaaagatc aatcattaaa cttaagcgtt tctcctttga
    31081 aaggtaaaat tggtaataat aaggatggat tagaatacct ccttttacca aaagataaaa
    31141 aaggtaaaac tctacagaaa tttataaata agcgtattaa agttttgaaa gaagatggta
    31201 ctttggcacg tttaagtaaa caatatttcg gtggagatta cgtttcaaac attgataaat
    31261 aaatcagtga gactataagt tttatctttt tacatgtgag agtttgcgtg atagaataac
    31321 gaagataaga aagtttagaa aaggaaagta gaaggaatga atcaatttat cttgacgggt
    31381 ggatggtctt ggtataataa tttggttagt caggttccag cggggaaatt atttagttgg
    31441 aaggctgttt tagatgcaat accctcaatt ttagagcgat tacccataac gctattattg
    31501 acagtagcag gagccttatt tggactcatt ttagctttga tttttgcagt agtgaaaatt
    31561 aaccgtgtca aaattttata tccgattcaa gctttatttg ttagtttttt acgtgggact
    31621 ccaatacttg ttcaattaat gctgagttat tatggaattc cacttttctt gaaattttta
    31681 aaccaaaaat atggttttga ttggaacatt aatgctatac cggcttctgt atttgctatt
    31741 actgcatttg cttttaatga agcggcttat acaagtgaaa ctatacgagc ggcgattttg
    31801 tcggttgatc aaggtgaaat tgaggcagca cgtagtttag ggatgacctc agcgcaagtt
    31861 tatcgtcgtg ttattattcc aaacgcagca gtagtcgcaa cacctacttt gataaacacg
    31921 ttaattggtc taactaaggg aacctcttta gcttttaatg ctggtattgt tgaaatgttt
    31981 gcccaagcac aaattatggg tggtagcgat tatcgctatt ttgaacgtta tatctctgtg
    32041 gcacttgttt actgggcggt tagtttctta attgaacaat tgggaaatgc tattgaacga
    32101 aaaatggcaa ttaaggctcc acaccattta actgatgaga ttccaggagg agtacgctaa
    32161 tgataaaatt gagacaattg actaaatcat tttctggtca aaaagtgtta gacaaacttg
    32221 atttagatat tgaaaaaggt caagttgttg ctctagtagg tgcttcaggt gcaggaaaat
    32281 caacctttct tcgtagtatg aattatttag aagagcctga ttatggaaca attgaaattg
    32341 atgattttaa ggttgatttt aaatcaatca gtaaagatga catactaaca ttacgccgta
    32401 agttagccat ggtttttcaa caattcaatc tatttgagcg tcgtacagct ttggataatg
    32461 taaaagaagg tttgaaaatt gtcaaaaaga tgtcagatca agaggcaaca cggattgcaa
    32521 gagacgaact ggcaaaggtc ggacttgcag atcgtgaaaa atattatcca agacatttat
    32581 caggtggaca gaaacaacgt gttgctttgg cacgcgcatt agccatgaaa cctgatgttc
    32641 ttcttttaga cgaaccgaca tcagcacttg atccagaatt agttggagaa gttgaaaaat
    32701 ctattgcaga tgctgctaag caggggcaaa ccatggtgct tgttagtcat gatatgaatt
    32761 ttgtttacca agtagcagat aaagttttat ttttggaaaa aggtcgtatt ttagagtctg
    32821 ggacgcctga acaattattt aaccatcctt tagaagaacg tacaaaagaa ttcttcgcta
    32881 gttataacaa atcgtaccta taagctaaaa tattttgata taatgaaggt tagaatgtct
    32941 aatcttcatt tttaattatt cttgagttag gctaagtata taagaaagct gcgatatgtt
    33001 ttgacaatcg cttgttggat agagatgttt ttagaaatag caaaagttta tttacaaggt
    33061 ttagggcttg ccagcataac tgttatttta gttggtatat tttgggtctt ttggcgtgcc
    33121 aagaaaaaca tagataaaac agctagagaa cgccaatctg ttctttatga tgtccttctg
    33181 atagctctta tgacagctcc aatacttgcc tttgcatttg tagctatcat tttaatgata
    33241 aaagcttagt cagattagtt ggaaatatag acgctttgct atagaataaa accataagat
    33301 tcaaaggaga aatagtaatg tatgatactt taataatagg ttcaggtcct ggtggtatga
    33361 cagcagcgct ttacgctgca cgttcaaacc ttaaagttgg gttaattgaa caaggtgctc
    33421 caggtggaca gatgaataat actgctgaaa ttgagaacta tccaggctac gatcatatct
    33481 caggtccaga attatcaatg aagatgtatg agccgttgga aaaatttgaa gtagagcata
    33541 tttatggtat agttcaaaga gttgagaacg atggtgatgt aaagcgtgtt atcaccgaag
    33601 atgaatcgta tgaagccaaa acagtaatcc ttgctaccgg agctaaaaac agccttttag
    33661 gagttccggg tgaagaggaa tatactagtc gtggcgtttc ttactgtgcg gtttgtgatg
    33721 gtgcattttt ccgtgatcag gatcttttag ttgtgggtgg cggtgattct gctgttgaag
    33781 aagctgtctt cttaactcaa tttgctaaaa gtgttactat tattcaccga cgtgatcaat
    33841 tgagagctca aaaagtactt caagaccgag cttttgctaa cgaaaaaata aaatttgtat
    33901 gggattcagt ggtcaaagag attaaaggaa atgagattaa agtatctggt gtaactgttg
    33961 aaaatctaaa gactggagaa atctcagaga tgactttcgg aggtgttttc atttacgttg
    34021 gtttgaaacc acattctagc atggtctcag aattaggtat tacggacgag actggctggg
    34081 tcttaactga tacaaatatg aaaacatcaa ttccaggact atatgctatt ggggatgttc
    34141 gtcaaaaaga tctacgtcag attgcgactg ctgttggtga aggtgctatc gctggacagg
    34201 gtgtttataa ctatataact gagcatttct aagaaatact aaaagtagaa aagactatcg
    34261 aaaaacgatt agtctttctt tttattttca tgaaagtaag gtgataaact gaactaccga
    34321 gatagaaata tgctataatg ttagacatat agccttatcc taggctcatg attaaaagga
    34381 gattgtctga tgtataaaga cgatagttta acactacata ctgatttata tcaaattaat
    34441 atgatgcaag tctattttaa taaaggtatt cacaataaaa aagcggtgtt tgaagcttac
    34501 tttcgcaagg ttccatttga aaatggttat gctgtttttg caggtttaga acgtattgtt
    34561 cgttatttag aaaatctgag cttttctgat agtgatttat cctatttaga agagttaggt
    34621 tatcctgaag aatttttaga ttacttaaaa aatttaaaaa tggaactgac tgtcaaatca
    34681 gctaaggaag gtgaccttgt atttgctaat gaaccattag ttcaaattga aggtccttta
    34741 gcacaatgtc agttagtaga gacagcgatt ttaaatatca tcaactacca gacacttgtt
    34801 gctacaaaag cagctcgtat tcgttctgtt attgaagatg aaccgctatt agagtttggg
    34861 acacgacgtg ctcaagaaat ggatgcagcc atttggggta ctagggcagc gattattggt
    34921 ggtgctaacg caacgagtaa tgttcgtgct ggtaaaatct ttaatattcc agtatcagga
    34981 acgcatgcac atgctttagt tcaaacttat ggcgatgatt accaggcatt taaagcttac
    35041 gcagaaactc ataaagattg cgtcttttta gtggatactt atgataccct tcgtgtaggt
    35101 gtgcctaatg ctattcgagt tgcaaaagag atgggcgaaa agattaattt cttgggagtt
    35161 cgccttgatt caggtgattt agcttatttg tctaagaaag ttcgtcagca gttagatgac
    35221 gccggttttc ctaatgcaaa gatttatgct tcgaatgatc tagatgaaaa tacaatcctc
    35281 aacctaaaaa tgcaaaaagc taaaattgat gtttggggtg tcggaacaaa attaattaca
    35341 gcctatgatc agcctgcgct aggtgctgtt tataaaattg tttctattga gactgacgcc
    35401 ggtagtatgc gagatacaat taaactatca aacaatgcgg aaaaagtatc aacacctggg
    35461 aaaaagcaag tttggcgtat caccagtcga gctaaaggga aatctgaggg tgattacatc
    35521 acatttgcag acactgatgt gacacaatta gatgaaattg acatgtttca tccaacttat
    35581 acttatatca ataaaacagt ccgtgatttt gacgcagtac ccttactggt agatatcttt
    35641 gacaaaggga aattagttta tcaattacca agccttcaag aaattcaaga gtacggacgc
    35701 aaagaatttg atcagctttg ggatgagtat aaacgtgttc tgaatccaca ggattaccca
    35761 gttgatttgg cacgcgatgt ttggcaaaat aaaatggacc tgattgatcg tatccgtaaa
    35821 gaagctttgg caaaaggaga agtaagatga ctttgcaaga tcaaattatc aaagaactcg
    35881 gtgtaaaacc tgttattaat ccaagtcaag aaatccgtcg ttccgtagaa tttttaaaag
    35941 actatctatt aaaacatagt tttctaaaga cctatgtact gggtatttca ggggggcaag
    36001 attcaacttt agcaggacgt ctagctcaac tagcagttga agagttgcgt gctgatacag
    36061 gggaaaacta tcaatttata gctattcgtt taccatatgg tattcaggcc gatgaagaag
    36121 atgcacaaaa agcattagat tttatcaagc cagatattgc tttgaccatt aatattaaag
    36181 aagctgttga tggccaagtt agagctctta atgcagcagg agtagagatt acagacttta
    36241 ataaaggaaa tattaaagct cgtcaaagaa tgattagtca atatgcagtt gcaggtcaat
    36301 atgctggagc ggttattggt actgatcatg ctgcagaaaa tattacaggt tttttcacca
    36361 aatttggtga tggaggtgcg gacttattac ctctctttag acttaataaa agtcagggaa
    36421 aacagttatt agctgaactt ggtgctgata aagacttgta cgaaaagatt ccaaccgcag
    36481 atttagaaga aaataaacca ggaatcgctg atgaaatagc acttggagta acctaccaag
    36541 aaattgatgc ttatcttgaa gggaaggtag tttcggacaa gtctcgaggt atcattgaaa
    36601 attggtggta taagggtcaa cataaacgtc atcttccaat cacaattttt gatgatttct
    36661 ggaaataaga aaaatagcta gaatattcta gctatttttg tttctctaat aaattacttt
    36721 cagacttgca gaaatattca gaatgtttat aatagtagct aagtataaaa ggaggaattt
    36781 atgtcagaat taactcaaac atttacagat aagttattag ctgattacca agcaaataca
    36841 aaatttagcg ctattgaaaa tgcagtaact cacaatggtt tattgaagtc tctagagact
    36901 cgtcaaagtg agattgagaa cgactatgtc ttttcaattg atttgaccaa agatgaagtc
    36961 tctaatcaga aacaatcagg acgttgctgg atgtttgctg cactgaacac atttcgtcat
    37021 aaactgattt cagattttaa actcgagaac tttgaattat ctcaggcaca tacatttttc
    37081 tgggataagt atgaaaaatc aaattggttt atggaacaaa ttattgcgac tgcaaatcag
    37141 gaattgagta gtcgtaaggt aaaattccta ttagatgtac ctcaacaaga tggcggacaa
    37201 tgggacatgg tggttgctct gtttgagaaa tatggtgtcg ttcctaaaac agtttatcct
    37261 gaatctgttt catcaagtgc tagtcgtgaa ctaaatcaat atttgaataa acttttacgc
    37321 caagatgcac agattctacg agaattaata gcgcagggag cagatggggc aactgttcaa
    37381 aataaaaagg aagaattgct tcaagaaatt tttaactttt tagcaatgaa ccttggcttg
    37441 ccaccgcaat cttttgattt tgcttatcgt gataaagaca atcattatca gtctgataaa
    37501 aatattacac cgaaagcatt ttatcaaaaa tatgttaatc ttgacatatc agattatgtt
    37561 tctattatca atgctccaac tgtagataag ccttatggac aatcctatac agttgagatg
    37621 cttggaaatg ttgtcggagg cccagctgtt aaatatttaa accttgatat gaaacggttt
    37681 aaagagttag cgattgcgca aatgaaatca ggagaaactg tgtggtttgg ttcagacgta
    37741 ggtcaagtat caaatcgtca aaagggtatc ctagcaacta cgacttatga ttttaattca
    37801 tctatggata ttaaacttag ccaagacaaa gcgggccgcc tagattatag tgaaagttta
    37861 atgacacatg ctatggtctt aacgggagta gaccttgatg aatctggaca acctttaaaa
    37921 tggaaagttg agaactcttg gggagagaaa gttggtaagg atggttattt tgtagcttca
    37981 gatgcttgga tggatgaata tacttaccaa atagtcgttc gtaaagaatt gcttactaaa
    38041 gaagaattag aggcttacaa cgcagagcca attactttag cgccgtggga tccgatggga
    38101 gcactagcta attagtaata gtaattataa attaaaaaac tggatatatt atccagtttt
    38161 tttaattacc gttaggtgct gtattgttat taccattacc atgtccagtt cctggcacag
    38221 tttgattgga attattaggg gtagtagcat taccattatt aggtgtttcg acttgtgggg
    38281 atgatgaatt ggcatcatct gctgtattct tatcctcttt ttgtttctca gaggatgttg
    38341 aactactgct ttgacttgac tgtccagaat tttgatagat attcttatag acagaagaag
    38401 agtaagtgcc ggttgtagtt gtcccattga tatacaagta gcttccactt cgatagagcc
    38461 cttctggcat tgtccaatct gcactgtatc cgccagttag gtaggacatc attgcacgat
    38521 aaacctcagt agcaatatcc agttggctac cataaagtgg tgtaaggcga tttttataac
    38581 cagtccaaat tgccattgtg tacttagaag tatagccgac aaagttttcg tcaggagcca
    38641 ttgtaccaac ggcgctattg tagataccag tagttgcttc aattttagct aactcatctt
    38701 ctgtatagtt ggaagtacca gtcttaccag cttgtgcaac accagggata gctgctttag
    38761 taccagtacc aaatgttagt actgttttca acatatccgt catcatgtag gcagtagtct
    38821 ctttcatcgc acggctacca gacgctgcat aagtatcatt ggttccatcg ctaaattcaa
    38881 ttttattaac atattgcggc ttatagtaag ttccgccgtt tgcaaaagcc gagtaagccg
    38941 ctgccatttt ctcactactt gcaccatatt ttgcatcact gctattgttg ttacttgaaa
    39001 tagcatttga atagttcatt tctggatagt atatgcctaa tttttcaagg aaagattttg
    39061 cttcgtctaa tccagcggct tcaagtgctc tgacagcagg gacgttacgt gattgttgaa
    39121 tagcggtctg catactcatc caacccatat attgacgatc ccagtcatat agttgagtag
    39181 aagtaccagg ccagtagtaa actgagtcgt ttaatgattg acctgttgaa ttatagacac
    39241 cactatcaat agcaggtgca taagctgaga taggtttcat tgtagaaccc caatcgcggt
    39301 ctgttaagac agattgattt gtcccaaatg aaatattttc attctgatga cgcccgccta
    39361 attgtgcaat gactttacca ttagtcgcat ccatgatggt agatgctatt tgtaattcat
    39421 tgtttggata agcgatgtaa gtatcactgt tgtagatgtc atatagttgt ttttgtgcat
    39481 cagtgttgat attagtatac acttttagcc cagcagtaaa gatatcttta ccagtttttt
    39541 gtttaacttc actaataact tgttttaagt agttatccat atattttgga taagtagatt
    39601 tttgctttaa ttcttttaag ccatcagtta ctggagttgc aacagcttgg tcgtattcct
    39661 ttttagaaat gtttttatct tgatacatct gctgaagaac ggtattacgt cttgtttgag
    39721 cagattctgg gtttttataa gggtcatatt gtgtaggtgc ttgaggaata ccagcgagca
    39781 aagcaagttg tgcaatagat aattccttta ggtctttacc aaagtatgat ttagctgttg
    39841 ttctcatacc ataattccca tttcccatat aaactttatt aatatagaaa gtaagaattt
    39901 cttctttggt gtatttacgc tccatttgaa gcgcaagcca aacttcctgt gatttacgtt
    39961 ttaacgtttg gtcagattta ttggtagaaa agtaagccag tttaatcaac tgttgatcaa
    40021 gggttgaacc accttgcgta ttactactaa caaggttatg ccaagctgca cctaaaatac
    40081 gataaatatc gacacctcta tgtttaaaga aacgtttatc ttctatagaa gtgatagcgt
    40141 taaccaaatt tagtggaatg ctatccgcac taacactttc acgcttttct gagcctaaat
    40201 cggcaataag tttattatta ccatcataaa ccaaactaga gttaacggat tttaaagctt
    40261 gatcggttaa tttcggagaa cgactaacgt agtatgcaaa taggagccca cctattacaa
    40321 tagctaagat aataaatccc attataatgc caaaagcata cttcaatagt ttgataacag
    40381 attccttttt aattgtaatc accgcctaat aatttttctt caataatatc taagtaaggg
    40441 acttgaggaa aggcactctc ctttacgaaa aatccatttt ttctgatata atcaataggc
    40501 attgatttat tgcctttatc aatctgataa aatgaaatta actcattagc agggagtaga
    40561 taggtttcct taagtgtgga aaaatgaagc aagacaaagc aaatcccttt ttgctgtaat
    40621 acatttgcca tgtgctctat ttggtgagca tgaaaatttt tcataggcat agcagttttc
    40681 tgccgggttt ctttggcttc aaagtcaata tagtaacctt tgtaaacacc agaataatca
    40741 gtagtagaag cttgtctaaa ataagcttct acaatcttgg cacgactacg tttaggataa
    40801 tctaccttga cgatttgtac tggcgttggt ttcttatgaa tgacagctaa ttcgtgagac
    40861 aaatagtagt cattggttgc attgatagca gcttcaaatg acataccacg gttggcaaaa
    40921 tcgattttct ttttctttga ttttgtaact gttgttttcc gaataagctg atgtggatag
    40981 ttaaccattt ttctccttgc attacaaata aaatgctaag ataagcaaaa ctattatacc
    41041 ataaaattag gatggacaac aaatgaagtc aactattctt gtaacaggtt acaaaaattt
    41101 tgaattaggg attttccagg ataaggatcc tcggattact attataaaaa aggctattga
    41161 taaggatttt agacgcttct tggaaaatgg agcagactgg tttattttta tgggaaattt
    41221 agggtttgag tactgggctt tggaagttgc tcttgattta caaaaagaat atgattttca
    41281 aattgcgaca atttttacct ttgagaacca tggccaaaat tggaatgagg ctaataaagc
    41341 taagttggca ctctttaagc aagttgactt tgttaaatat actttcccaa gctatgaaaa
    41401 cccgggtcaa tttaaacaat acaatcattt tctcattaac aacacacaag gtgcatatct
    41461 tttttatgat tctgaaaatg aaactaattt aaaatttttg ctagaaatga tggaaaaaaa
    41521 ggaagcttat gatatttctt tcctaacttt tgatcgctta aatgaaatat atgaggaatg
    41581 atcgtttttt cggatatgag aacgttttac gcttgatttt tccatttaat ttaggtatta
    41641 tttactttga tataaaggat aggacttata ttaaaacgat ttgactggtt tggagaatag
    41701 aatggcaagt attatttata gcccaaaaga tatttttgag caagatttta aagttagtat
    41761 gcggggatat gacaagaaag aagtcgatgt atttctggat gatgtcatca aagattacga
    41821 aaattatctt gagcaaattg agaagctcca aatggaaaat agacgtctac aacaagcttt
    41881 agacaaaaaa gaaagcgaag cttcaaatgt aagaaattca gggacagcta tgtataatca
    41941 aaaaccgatt gcccaatctg caaccaattt tgatatttta aaacgcatta gccgccttga
    42001 gaaagaagtt tttggaagac aaattagaga ataaggattt ctcaaatatg caatttttgg
    42061 ataatcgcgt agtattgata tactatgagg aaagtccatg ctagcactgg ctgtgatgcc
    42121 agtagtgttt gtgctaggcg aaaaaataag cctagggaga tagctagcta tcttacggca
    42181 agcaaaaggg ctaagtcttt ggatatgcct gaatagcttt gaaagtgcca cagtgacgta
    42241 gtttctaggg aaatctagaa agtggaacgc ggtaaacccc tcaagctagc aacccaaact
    42301 ttggtagggg catgggatag ttggaatgag aacaatctat cctgactgct ttgcagtaga
    42361 cagatgatta tcgaaagaaa caattcctag ttgtttctgg aacaaaacat ggcttataga
    42421 aaattgcata ataggttagt ggctgagata acatctcagc caatttttaa ctgtgatgaa
    42481 tacgatagtg taaaagagga aaaatgaaag aatcattcaa attaatagca actgctgctg
    42541 ctggtttaga agccattgtt ggtagagaaa tcagaaacct aggaattgac tgccaagttg
    42601 agaacggtag agtccgtttc cacggagaca tcaaaacaat cattgaaacg aacctatggc
    42661 tacgagctgc cgatcgtata aagattattg taggagagtt tccagcacgt acttttgagg
    42721 aactttttca gggtgtatat ggccttgact gggaaaatta tttaccgtta ggggcaaaat
    42781 ttcctattgc taaagcaaag tgtgtcaagt caaaattgca caatgaacct agtgtacaag
    42841 ccatttctaa aaaagctgtt gctaagaaac ttcaaaaagt ctttcaccgt cctgaaggag
    42901 ttcccttaca agaaaatgga gctgaattta aaatagaagt ttctatttta aaagataaag
    42961 cgactgtcat gattgatacg actggatcaa gtctcttcaa acgtggttac cgagcagaaa
    43021 aaggtggtgc gccaattaaa gaaaacatgg cagcagctat tattcaatta tctaattggt
    43081 tccctgataa gccattgatt gatccaactt gtggttcagg tactttttgt attgaagccg
    43141 ctatgattgg aatgaatata gctcctggtt ttaatcgtga ttttgccttt gaggcgtggc
    43201 catgggtaga tcaaagtcaa gtacaaaagg ttcgcgatga agctgagagt aaagcaaact
    43261 atgatattga tttagatatt tcaggttttg atttagacgg tcgtatggta gaaattgcta
    43321 gaaaaaatgc agaagaagca ggacttggag atgttatcaa attgaaacaa atgcgtcttc
    43381 aagatttaaa aacagataaa attaacggtg ttattatctc caacccacca tatggtgaac
    43441 gactcctgga tgacaaagca gtggacattt tgtataatga gatgggacaa acatttgcgc
    43501 ctttaaaaac ttggagcaag tttatcctaa ctagtgacga aggatttgaa aaaaaatacg
    43561 gctcacaagc tgataaaaaa cgtaagcttt acaatggtac tttaaaagtt gatttgtacc
    43621 aatattacgg tgaacgtgtg agacgtcaag taaaataacg gggagaactc atgtcagaag
    43681 atcaaaaaca cccatttttt gaaccagaaa aagaaactga tggtcttgaa tttaaagatg
    43741 ctaaagaaat gacagtagaa gaggctgtta gaaaagattc tgaaattaag gctggtatta
    43801 ccgaagaaga tagtattctt gataaataca tcaaacaaca tcgtgatgaa gtagcttcac
    43861 aaaagtttga gacaaaatca agcgattttg caaaccttga tacggcatct ttagatgatt
    43921 ttatcaagaa acagcgcgaa gaattatcag cgatgttagc tgcagaagag ctttctaaaa
    43981 agttggataa ttcagtctct caagaacaag atatagaagc taatgctgtc tcacctaaag
    44041 aagaatcttc acaagaacaa gaaaatagtg taactccagt tcctcctctg aatacggaag
    44101 cagaaccaac tgcaacagaa ccagattcga ctattgctga ttcagaggag tataaatcct
    44161 cttctaagaa gcgtggaggt attgtaggaa cattgattgc tcttatttta cttctaatag
    44221 taacaatctt tggttacaac tatttcaaga ataataattc gacaaattct caaactgcaa
    44281 caagtcaaag ttctagttct aaggctacga cgacttcatc agaagaagat aaaaaagcta
    44341 gtcagaactt agataatttc aacaaagctt atgctaactt ttttgttgat gataagaaaa
    44401 cccaactcaa aaatagcgaa tttgataaat tgtcagaatt agaaaagaaa gttgatgctt
    44461 taaaaggaac taaatactac ggtaaagtaa aagttaaatt tgatagtcta aaacgtcaaa
    44521 ttgatgctgt taaagctgtt aacgacaagt ttaagtcgcc tgctgtagtt gatggtaaga
    44581 agagtgaaaa attagaagta aaagatggag cgaattttga ctctttagat agtaaaactc
    44641 ttaatactgg taatgctagt ctagatagtt tacttcactc catcgtttca actggtcgta
    44701 atcaggtaaa acaatccgaa gaacaggcta aaaaaactca agaagaacag tcttcgtcta
    44761 ataaggtttc agacactcaa acaactgaac aaccaaacgt aactaacggt caatcttcct
    44821 catcagctgc tacaataaat aatcaggcag ctggcactgc atcaggtaat ttggaacgta
    44881 accgtagccg agtaccgtat aataatgctg ctattgcgga cacaggaaat cctgcctgga
    44941 tatttaaccc aggagtatta gagaaaatcg tggccacttc acaggcgcga ggctatttct
    45001 ctggaaataa ctatattctt gaacctgtaa atattatcaa tggtaatggt tattacaata
    45061 tgtttaaatt agatggcact tatttgttct ctatcaatgc caagacaggt tactttgtag
    45121 gtaacgctcc tggaagagct gatagtttag attattaata taaagagcaa aaccagtttt
    45181 ttgtctacac cctaaaagtt agaaaatcag tctaacgatt agggtgtttt ttgtattcgc
    45241 atttagatgt gtacgactgc tatgaggata ttagggaaaa attaaaagga ttttgctctc
    45301 tatgatacag aacaaaatcc tttgtttaaa ttaataagac aacttttgga attccaaaag
    45361 ttgactttta ttttttatta gatcagacaa tgtggcgctc aaaagcattg tcagaaatcc
    45421 cttgggaaag aattaatttc gcccattctt gagcagagtg gagactgtgg tctttataat
    45481 ttccacaaga ttcaatggtt gttccaggaa cgtcttccca agtgacccca ccagcaatag
    45541 cctctagact agatttgatg actttagcaa tttcagtagc atcctgtttt ccccacataa
    45601 tcatgtgaaa accagttcta caaccaaaag gagaacaatc aatcaaacca ttaatttgtt
    45661 ggcggattaa cttcgccaga aggtgctcga tagtatgtaa accagcagta tcaatagcat
    45721 tttcattagg ctgaataagg cggatatcaa agttagtaat gatgtctccg accggtccaa
    45781 cttcttctga aattaaacga acgtagggtg ctttaacgat ggtgtgatct aattcaaaac
    45841 tctctacgac aacttctttt gtcataagtt ttctcctttt gtattacatt ctagagacta
    45901 aaggagatgt tgaaaatttc tcctaatccc tttaaataac attctaatca tatcataaat
    45961 gtctataatt ttaaattgtg aaaacgcctt ttttatgata aacttaaaag tggactttct
    46021 atatgaaatc tagaaaaaaa ttgaggtata aacatgttta atattatatt agcaatggtt
    46081 tgtgcactca ttggtttgat tattggttat gtggctattt caatgaaaat gaaatcatca
    46141 aaagaagctg cagaactaac tcttttaaat gcagaacaag atgccgttga tttacgtggt
    46201 aaagcagaaa ttgaagcaga acacatccgt aaagcagcgg aacgtgaaag taaagctcac
    46261 caaaaagaat tacttttaga agcaaaagaa gaggctagaa aatatcgtga agagatcgaa
    46321 aaggaattta aatctgatag acaagagttg aaacagatgg aggcacgttt aacagatcgt
    46381 gcatcatctc ttgatcgtaa agatgaaaat ctatctaaca aagaaaaaat gttagatagc
    46441 aaagaacaaa gtctaaccga taaatctaga catattaatg agcgcgaaca ggaaattgct
    46501 actttagaaa caaaaaaagt agaagaattg tcacgcatcg cagagcttag tcaagaagaa
    46561 gctaaagata tcattttagc tgataccgaa aaagatttag ctcatgatat tgcgacacgt
    46621 attaaagaag cagaacggga agtaaaagat cgatcaaaca agatagcaaa agacttgcta
    46681 gcccaagcta tgcaacgttt agctggtgaa tatgttactg aacaaacaat tacaactgtt
    46741 catctccctg atgataatat gaagggacgt atcattggtc gtgaaggacg taatattcgt
    46801 actctggaaa gtttgacagg tattgatgtt atcatagatg atacgccaga agtcgttgtc
    46861 ttatcaggtt tcgaccccat tcgtcgtgaa attgctcgta tgactttaga atctttgatt
    46921 caagatggac gtatccatcc agctcgtatt gaagagttgg ttgaaaaaaa tcgtttggaa
    46981 atggatcaac gtatccgtga gtatggtgag gcagcagctt atgagattgg tgcaccaaat
    47041 ctgcatcctg atttgataaa aattatggga cgtttgcagt tccgtacatc atatggtcaa
    47101 aatgtacttc gtcattcagt tgaagttggt aaacttgcag gtattttagc tggagaacta
    47161 ggagaaaatg ttgatttagc tcgtcgggct ggcttcctcc atgatatggg gaaagctatt
    47221 gatcgcgaag ttgaaggcag tcacgttgaa attggtatgg aatttgctcg taagtacaaa
    47281 gagcatccga ttgttgttaa tactattgca agccaccatg gtgatgtaga acctgacagc
    47341 gttattgcag taattgttgc tgctgcagac gcccttagtt cagcacgacc aggtgcgcgt
    47401 aatgagtcaa tggaaaatta tatcaaacgt ctgcgtgatt tagaagaaat tgctaatggc
    47461 tttgagggag tgcaaaatgc ctttgcactt caagccggac gtgaaattcg tatcatggta
    47521 caacctggta aagtttctga tgatcaagta gttattatgt cgcataaggt acgtgaaaaa
    47581 atcgaacaaa acttggatta tccaggtaat attaaggtaa cagttattcg tgaaatgcga
    47641 gctgttgatt ttgcaaaata gtcaaaagga gagcttgtta gctctccttt tgttatagag
    47701 ttgacatttg tcatattttg aaatgacaaa aatatctaaa aagtttcctt ctgatttttt
    47761 atactatgat tatagtaaag gagaaccaaa tggcaaaaga tataattaag gttgatcata
    47821 tatttaaaag catcgggcaa aaaacgattt tagaagatat atcatttagt atagcttcta
    47881 accaatgtgt agcattgatt ggacctaatg gtgcagggaa aacaactctg atgtcaactt
    47941 tattaggtga tattagtatt tcttcaggag cattgactat ttttaatctg cctgcacacc
    48001 acaataggtt aaaatataag gtggccatct taccacaaga aaatgtattg ccctcaaagt
    48061 ttacagtgag agaactcatt gatttccaaa ggtgtttatt tccagaagtg ctgccaatgt
    48121 ctctgatttt agactatcta cagtggagtg atgcgcactt gcaacaattt acagagactt
    48181 tatctggagg gcaaaaacga cttttagctt ttgttttaac tttggttggt aagccccaat
    48241 tactattttt agatgaacct acttccggaa tggatacttc cacacgtcaa cgattttggg
    48301 agctggttgc gacactaaaa aaagaaggtg acacaattgt ctattctagt cattatatcg
    48361 aagaggtaga acatacagct gataggattt tagtacttca taaaggaaag ttattacgcg
    48421 atacaacccc ctttgccatg aagcaagaaa aaaccgaaaa gttattcacc gttccgctta
    48481 gttatcaaaa attattacct acctatttga ttacagagtg tgaagccaag agtgatagta
    48541 taacgtttgt tactggggag gctgaaactg tatggaaaat actggcagat aatggttgtc
    48601 ctattgaagc tattgagatg accaatagaa ctttgttaaa tcgtattttt gagactacta
    48661 aggaggtaaa acatgagaat ctttaaagca ttattgaaaa tggaatttct tttgacaaaa
    48721 cgtcaacttg ccaatctcat tatggctata ggtatgccag tggctttttt tctatttttt
    48781 tcaggattta tgggcgaagg actcactaaa gccatcgaag ctatctacgt tcgtaattat
    48841 atgataacga tggcaggatt ctcaagtctt agctttgctt tttttacttt tccattttct
    48901 atgaaagatg atcaactctc aaaccgaatg caattattaa gacactcacc agtaccaatg
    48961 tggcaatatt atctagcaaa aattattcgt attttatttt actattgcct agcaataact
    49021 gttgttttcc taacagggca tattttaagg caagttagta tgccaataga acaatggatg
    49081 cagagctttt tattgctatt aggaggagca acatgtttta ttccttttgg attgctcgtc
    49141 tcctacttta aaaatactga actaatgtca atggtagcca atatttgtta tatgtcatta
    49201 gctgttcttg gtggcatgtg gatgccaatt acaatgttcc caaaatggtt gcaaggttta
    49261 tcaaagttaa cccctactta ccatttgact caagttatcc ttagtccgtt tgctaatagt
    49321 tttgctgttt tttctcttat tattctgata ggctatggta ttataatgtt agttatcgct
    49381 tatctactat ctcagaagag gcattcaatt tgaaaaataa aaaaaataac tatgatttaa
    49441 tgtattttat tccgttagta tttttgattt accccatagg agggattctc tattatcact
    49501 accctttttg gactttattt tttacgctag cttttgtagg agcatattta tactctatta
    49561 ttatcagggg cgaatcaaag tatcacatga ttgcctggtt tacaatgttg acatatattt
    49621 tctacatgac agtttttata aatagtggtt tcatctggta tatttacttt ctgagtaatt
    49681 tactcgtcta tcgctttaga gataaattga agtcttttag attcattagt tttgcttgca
    49741 ctctagccac aatggtattt ttatgcttct ttaaggcatc agactttggt gatagaatca
    49801 tgtttctaat tgttcctatt ttttgtatcg gttacatgtg gattgctatt gaaaatagaa
    49861 atagcgaaga acaaagagaa aaaattgcag agcaaaatca gtatatcaat attttgtcag
    49921 ctgaaaatga acgtaaccgt atagggcgtg atttgcatga tagtttagga catacatttg
    49981 ctatgatgac actaaagaca gaactagcgt taaagttgtt agaaaaaagg aactatgata
    50041 aggtacaaaa agaactatca gaattaaatc atattagtca tcaatcgatg agtgaggtcc
    50101 gtcaaattgt ttctaacctc aagtatcgta cagtagtgga agaaattgat gagttgtacc
    50161 gcttgtttca attatctaac atcaagttaa cagttgttaa taagttagag acgtcacaac
    50221 taagtccagt aacacaatct acaatcacaa tgatattaaa ggaattatcc aataatattg
    50281 ttaagcatgc tgaagctgat agcgtggagt tgagtttagt aaggcaaggt gctactataa
    50341 atattgagat gattgataat ggttgtggtt ttacaaacct tgacggagat gagttgcata
    50401 gtattcaaga gagattaact atcgttgaag ggacactaac gattttgtcc cgaagtaaac
    50461 caactcatat tcaggtgata ttgaaagagg tataagatga aattacttgt tgctgaggat
    50521 cagtccatgc tacgagacgc tatgtgccaa ttacttttga tggaagaaag tgtttctact
    50581 attgatcaag ctggaaatgg tgaagaagct attgccattt tatccaataa agccatagat
    50641 gttgcaatat tggatgttga gatgccgata ttatcaggtt tagatgtctt agagtgggtt
    50701 agaaaatacc agaatgttaa ggtcataatt gtaacaacct ttaagcggtc aggttatttc
    50761 caaagggcta ttcgttcaaa tgtagatgct tatgttttga aagaccgctc agtagctgat
    50821 ttgatgaaaa cgattcaaaa agttttatct gggggtaaag aatattcgcc tgaattaatg
    50881 gaaaacgtta tatctaatcc tttgagtaag caagaaataa aaattctttc cctaattgct
    50941 caagggaaga ccagcaaaga aattgcatgc actttatttt tatcgaatgg tacagttcgt
    51001 aattacacgt caaatatttt tgataaatta ggagttaata atcgtttgga ggcacttaaa
    51061 atagcacgag aaaaggattg gctgtgatag tttttttcca gtcgagatgc cataatgtat
    51121 taagagggat atatgtatgc aaaaatttaa agaacgtttt gatacgctta gtgatgcaat
    51181 tttggccatt gctatgacga ttttagtttt ggagataaaa actccgacta cgatgggtga
    51241 tattggtgac tttacacgga acataggttt atttattgtt agctttgttg ttgtttttaa
    51301 tttttggtac gaaagggcac aaaatagcct agatgctcaa aagactaatg atgaaattat
    51361 agctcttgat atcattgaac acctaggtat ttgtttgatt cctctcttta ctaaatttat
    51421 gatttctttt gagaatcata attttgctgt tatggcttat ggactcttaa cgttattagt
    51481 tggtctcaca tcagacatta ttcggattcg tttggcaagc tatgatttag tgaccattcc
    51541 tagtgaattg aaagagcggg ttataaaagt catgacaact tttgccattc gttcagttgt
    51601 ggttcgcttt atcattatta tcctcgctta ttttctacct gaggtaggga tatttgcata
    51661 tttagtcata ccgctcttta tgttttggcg tcgctctaga gcaggaaaat actatactaa
    51721 aaagggcatt gaggcaccct cttatatcaa gctttttatc caaattggag agagagttcc
    51781 tgaggatttt actaattcaa gtagtggaat aagtggggta aaaagactct ataaaaaata
    51841 ataggagacc ttgaaaaaaa ggtttctttt tgttaaacta atgagaagaa actttattta
    51901 gggagcaatt tatgaattgc tataaaagtt aatagttaag gtaagaacca gattgttctg
    51961 gtctttttaa cacttaacac ccgatctatt aatttgagga gtgtgtaatg tctgaacgtg
    52021 gtttgctaat tgttttctca ggaccatctg gtgttggtaa ggggacagtt aggcaagaaa
    52081 ttttttcaac ccctgaccat aaatttgatt attccgtttc aatgactact cgtccacaac
    52141 gcccaggcga agttgatgga gttgattatt tcttccgtac acgtgaagag tttgaagcgt
    52201 tgattaaaga aggtcaaatg cttgagtatg cggagtatgt tggtaactac tatggaactc
    52261 ctttaagtta tgtcaacgag actttagaca aagggattga tgtttttctt gaaatagaag
    52321 ttcagggagc gcttcaagtt aaatcgaagg ttccggacgg tgttttcatt ttcttaacac
    52381 caccagattt agaggaatta gaagagcgtt tagtaggacg tgggacagat agtccagaag
    52441 tgattgccca acgtattgaa cgtgccaaag aagaaattgc tttgatgagg gagtatgact
    52501 atgctgttgt caatgatcaa gtgtcattag cagcagaacg tgttaaacgt gtcattgaag
    52561 cagaacatta tcgtgtggat cgtgttattg ggcgttatac caacatggtc aaagaaactg
    52621 ataaaaaatt aagttaaaaa tagaaaagag aattgcctta tgatgttaaa accttctatc
    52681 gacactttgt tggataaagt tccttcaaaa tattctttag ttattttaca agctaagcgt
    52741 gctcatgaat tggaagctgg tgagaaagcc actcaagatt ttaaatctgt gaaatcaaca
    52801 cttcgcgctt tagaagaaat cgagtcaggt aatgttgtaa ttcacccaga tccatcagca
    52861 aaacgtgcct cagttcgcgc tcgtatcgaa gctgaacgcc ttgctaaaga agaagaagaa
    52921 cgtaaaatca aagagcaaat tgctaaagaa aaagaagacg gcgaaaaaat ttaagcaagt
    52981 caaaagtgag aggttgaaac attctgtatc agcctctttt catattttag agaattaggg
    53041 agggtttatg gcaagaaaat tagcacaggt tattgttgat attcctctga tgcagacaga
    53101 taaaccattt tcgtatgcca ttcctaaaga tttggaagat ttggtacaag taggagcacg
    53161 cgttcatgtt ccttttggaa gagggaatcg tcttttgcaa ggctttgttg tcggtttcag
    53221 agatgatgat gaattagaga ctaaggatat tgcgggggtc ctagattttg aacctgtttt
    53281 aaatcaggaa caactagact tagctgatca gatgcgccat actgtctttt cttataaaat
    53341 atctattttg aaatcaatgt taccaagtct tttgaattcg caatatgata aactcttgtt
    53401 agcaacagac acattaccat cagaagatag agaagatcta tttggtcata aaacggaaat
    53461 tgtcttttca agtttgtctt ctcaagatgc caaaaaagca ggcagattaa ttcaaaaagg
    53521 tttcatagaa gttcaatatt tagccaaaga taagaaaaca ataaaaacag aaaagattta
    53581 caaaatcaac agaactctgt tagaagagag tcaaatagca gcaagagcga aaaagagact
    53641 ggagttgaaa gaattcctgt tagaaaatcc tcaaccaggg cgcctcacag ctttaaacaa
    53701 acaattttca tctcctgtag ttaatttttt cagagaagaa ggaatcattg aggtcattga
    53761 aaaagaagct agtagatctg ataattattt taaaggaatc ctaaaaacgg attttttgga
    53821 tttaaatcag gaacaagcta aagtagttaa aatagttgtt gaccgaattg gtaaggatca
    53881 gaataaaccc ttcttgttgg aggggattac agggtctggt aagacagagg tatatcttca
    53941 tgtcattgat aacgttttaa agttgggaaa aacagcgatt gttctcgttc cagaaatttc
    54001 tttaactccc cagatgacga atcgttttat ttcccgtttt ggaaagcgag ttgctattat
    54061 gcactcgggc ttgtcagatg gtgaaaaatt tgatgagtgg cgaaagataa aatcaggtca
    54121 agctaaagtt gtcgttggag ctcgttcagc aatttttgct cctttggaaa atatcggagc
    54181 catcattata gatgaagaac atgaatcaac ttataagcaa gaatctaacc caaggtatca
    54241 tgcgcgcgat gtggccttgc tacgtgcaga atatcataaa gctgtgttac taatgggatc
    54301 agcaactcca agtatcgaaa gccgagctag agctagtcgg gatgtttata aatttttaga
    54361 attaaaacat cgtgctaatc ctaaagcacg tattcctcag gttgaaatta ttgattttag
    54421 aaattttatt ggtcaacaag aagttagtaa tttcacacca tatttactag ataaaatccg
    54481 agatagatta gataagaaag agcaagtggt tcttatgctc aatcgtagag gttattctag
    54541 ttttattatg tgtcgaaact gcggctacgt tgatcaatgt cctaattgtg acatttctct
    54601 gactttacat atggctacaa agaccatgaa ttgtcattat tgtgggtttg aaaagcctat
    54661 tccaagaaca tgtccaaatt gcaatagtaa aagtatctct tactatggta ctggaaccca
    54721 aaaagcttat gaagaacttt tgaaagttat cccagacgca aagattttac ggatggatgt
    54781 tgatacgaca aggcaaaaag gcggtcatga gtctattttg aagcgtttcg gaaatcatga
    54841 agctgatatt ttattgggaa cacagatgat tgctaaggga ttggattttc caaatgttac
    54901 tttagttgga gtactaaatg cagatacttc tctgaattta cctgattttc gatcatcaga
    54961 gcgaactttt cagctattaa ctcaagtggc tggacgtgct gggcgtgctg aaaaagaagg
    55021 agaggttgtc attcaaactt ataatcctaa tcattatgct attcaattag cccaaaagca
    55081 agattttgaa gctttttatc aatatgaaat gaatatccgt cgacaacttg gttatcctcc
    55141 ttattatttt acagttggtt taacattatc gcataaagat gaagagtggt taatacgaaa
    55201 atcttatgaa gtattaagtt tattgaagca aggctttagt gataaagtaa agttgttagg
    55261 tccaacgcca aaaccaattg caagaacaca taacctttat cactaccaaa ttattatcaa
    55321 gtatcgattt gaggacaacc ttgaacttgt tttgaatcgc ttattagaca tcactcaaga
    55381 caaggagaat agagatcttc gcttagcaat agaccacgaa ccgcaaaata tgatgtagaa
    55441 tagtgctatg atataattga aaaaagaaag acgaggaaaa agcaatgaca aaattattat
    55501 tcatgggtac ccctgatttt tcagcgacgg ttttaaaagg tattttagca gatggtaagt
    55561 atgatgtttt agctgttgtg acgcaaccag atcgtgctgt aggtcgtaaa aaagaaatta
    55621 aaatgacccc tgtaaaagaa gtggccttgg aaaacaacat tcctgtgtat cagccggaaa
    55681 aactatctgg aagtccagaa ttagagcaac taatgaccct aggtgcagat ggtattgtaa
    55741 cagctgcttt tggtcaattc ttaccaacaa agcttttgga atcggtaggt tttgcaatta
    55801 atgtacatgc ttctctatta ccaaaatatc gtggtggggc accaattcat tatgccatta
    55861 ttaatggcga aaaagaagct ggggttacca tcatggagat ggttgcaaag atggatgcag
    55921 gggatatggt atcaaaagct tccgttgaga taactgatga agacaatgta gggacaatgt
    55981 ttgatagact cgccgttgtt ggtcgtgacc ttttattaga tacgcttcca ggttatctat
    56041 caggtgatat taagccgata ccacaaaatg aagaagaggt aagtttttct cctaatatta
    56101 gtccagatga agagagaatt gattggaata aatcatcgcg cgacattttt aaccatgttc
    56161 gagggatgta tccatggcca gttgcacata cccttttgga gggaaatcgt tttaaacttt
    56221 atgaagtaac aatgtcagaa ggaaagggct caccgggtca agtgatcgct aagacaaaga
    56281 actcattgac agtagcaact ggtgacggag ctattgaact caaatcagtt caaccagcag
    56341 ggaaacctcg tatggatata aaagactttt taaatggtgt tggtcgtaat ttagaaatag
    56401 gagataaatt tggcgaatga ttggaaaaag agtgcgcgtg gtttagcttt aatgacttta
    56461 gaagaagttt ttgataaagg agcttattct aatatcgcac ttaataagag tttaaaaaaa
    56521 tctagactat cagataaaga tcgtgcctta gtaacagaaa ttgtctatgg aactgtggct
    56581 agaaaaataa cgctagaatg gtatctatca cactttatag ttgatcgtga taagcttgaa
    56641 ttatgggtat atcatctctt gctactgagt ctttatcaac tactctatct ggacaacatc
    56701 ccagatcacg ctattgttaa tgatgctgtc actattgcta agaatcgagg caataaaaag
    56761 ggtgctgaaa agttaattaa tgccgtttta cgaagagtaa gttcggagac gctccctgaa
    56821 attgcatcaa taaaacgcca aaacaaacgt tactctgttg cttattccat gccagtgtgg
    56881 ctcgtaaaaa aattaattga ccaatatggt gagacaagag ctttagcaat tatggagagt
    56941 ttatttgagc gaaataaagc cagtcttcgt gtgactgact tatcgcaaaa acaaactatt
    57001 aaagaaactt taaatgttag agattcacat attgcagaga cagctttagt tgcagactca
    57061 ggaaattttg catcgacatc atttttccaa gatggcctca ttactatcca agatgaatct
    57121 agtcaattag ttgctccaac actcaaagtc tcgggtaatg accaagtatt ggatgcatgt
    57181 agtgcaccag gaggaaaaac aagtcatatc gcctcatatt tgactacagg ggcagtaact
    57241 gctttagatt tgtatgatca taaattggaa ttggttatgg aaaatgcaaa acgcctaggt
    57301 ctgtcagata agataaaaac taaaaagtta gatgcaagta aagctcatga gtattttcct
    57361 gaggatactt ttgacaaaat tttagtagac gcaccttgtt caggtatcgg gctaattcgc
    57421 cgtaaaccag atattaagta caataaagct aatcaagact ttgaagccct ccaagagatt
    57481 caattgtcta tcctatctag cgtttgtcaa acgctacgta aaggtggtat aataacctat
    57541 agcacttgta ccatttttga ggaagaaaat tttcaagtta ttgaaaaatt cttagaaaac
    57601 caccccaatt ttgagcaagt agaactgagt catacacaag aggatattgt aaagagaggc
    57661 tgtattagta taagtcctga acaatatcat acagatggct tttttattgg acaagtcaaa
    57721 cgtatcttgt agtgtctagg aggaaactga catcgtcaga aaaaaatata tggaaatatc
    57781 tcttttaaca gatattgggc aacgtcgttc caataatcaa gattttatta atcaatttga
    57841 aaataaagcc ggcgtcccac taatcatctt agcagatggt atgggaggac atcgtgcagg
    57901 aaatattgct agcgagatga ctgtaactga tctaggaagt gattgggcgg agacagattt
    57961 ttctgagtta tcagaaattc gtgattggat gcttgtctct attgaaactg agaatcgcaa
    58021 aatatatgaa ttaggtcagt cagatgatta caaaggtatg ggaactacca ttgaagcagt
    58081 tgccattgta ggagataata ttattttcgc acatgtagga gattcacgta tcggtatcgt
    58141 tcgtcaaggg gagtatcatt tattgacgag tgatcattct cttgttaatg aattggtaaa
    58201 agccggccaa ttaacagaag aagaagctgc tagtcatcct caaaaaaata ttattacgca
    58261 atcaattggt caagccaatc cagtcgaacc tgatttagga gttcatctac ttgaagaagg
    58321 tgactacctt gtagttaata gtgatggact aactaatatg ttgagtaatg ccgatattgc
    58381 tacagtacta acacaagaaa aaaccttaga tgataagaat caagatttaa tcactctagc
    58441 aaaccataga ggaggtcttg ataatatcac tgtggcacta gtttacgttg aaagtgaggc
    58501 ggtttaatga ttcagattgg caaattattt gctggtcgtt atcggatcct taaatcaatc
    58561 ggacgaggtg gaatggcgga tgtttatcta gctcgagatc taattttaga taatgaagag
    58621 gtcgcaataa aagtattgcg aaccaattat caaacagatc agattgcggt tgctcgcttc
    58681 cagagagaag cacgagcaat ggctgagctg acacatccta atattgtcgc tattcgtgat
    58741 ataggtgaag aagacggaca gcagttctta gtaatggaat atgttgatgg attcgattta
    58801 aaaaagtata ttcaagacaa tgcaccccta tcaaataatg aagttgttcg aatcatgaat
    58861 gaagtccttt cagccatgtc actggcacat cagaaaggta tcgtacaccg tgatttaaaa
    58921 cctcaaaata ttttattaac taaaaaaggg actgtaaaag ttacagattt cggtattgcg
    58981 gtagcgtttg ctgaaactag tttaacgcaa acgaattcta tgttaggtag tgttcattat
    59041 ttatcgccag agcaggcgcg tggttcaaaa gcgactgttc agagtgatat ttatgccatg
    59101 ggaatcatgc tttttgaaat gttaactggc catattcctt atgatgggga tagtgcagtt
    59161 actattgccc tacagcactt ccaaaaacca ctaccatcaa tccttgcaga aaataaaagc
    59221 gttccacaag ctttggaaaa cattgtcatt aaagcaacag ctaaaaagct aacagatcgc
    59281 tataagacaa cttatgagat gggacgtgat ttatcaactg ctctaagctc gacacgtcac
    59341 cgtgaaccaa aacttgtttt taatgatact gagagtacta agacactacc aaaagtaaca
    59401 tctacagtat catctttaac gacagaacaa ctgttgcgta atcaaaaaca ggccaaaaca
    59461 acagaaaaaa taactcctga ttctgcgagt aacgataaaa cgaagtctaa aaagaaggct
    59521 tctcatcgac ttcttggtac aatcatgaaa ttattctttg cactctgtgt tgtaggaata
    59581 attgtatttg cttataaaat tttagtaagt cctacgacta ttcgtgttcc agatgttagc
    59641 aacaaaacag tagctcaagc aaaaatgaca ttggaaaatt ctggattgaa agtaggagct
    59701 attcgtaata ttgagagtga tagtgtttca gaaggccttg tcgtaaaaac agaccctgct
    59761 gcaggacgtt ctcgtagaga aggggctaaa gttaatcttt atattgccac tccaaacaag
    59821 agtttcactc taggtaatta taaggaacat aattacaaag atatcctcaa agatcttcaa
    59881 ggcaaaggtg ttaaaaaatc tttaatcaag gttaagcgta aaattaataa tgattatact
    59941 actggtacaa tcttagcaca gtcgcttcca gaaggaacat cttttaatcc tgatggtaat
    60001 aagaaattaa cacttacggt tgctgttaat gatccaatga ttatgcctga tgtaacaggt
    60061 atgacagttg gagaagtaat agaaaccttg acagacctcg gattagatgc agataatctt
    60121 gtcttctatc aaatgcaaaa tggcgtttat caagcagtag taacacctcc atcttcaagt
    60181 aagatagcaa gtcaggatcc ttattacgga ggagaagttg gattaaggag aggagataaa
    60241 gttaagctgt atcttcttgg ttctaaaaca acaaataact catcatctac gccaatagat
    60301 tcttcggctt caagctcaac aggaacaact acctcggatt ctgtaagctc atcaacggat
    60361 gctagcactt ctgatagtag tagcacttct acaagtagtt caacactacc aagtgatagt
    60421 acaactaata caggaacagc taacaatcca ttaactcaat aaaattaaaa agaagatgtt
    60481 aaagtcttct ttttaatttg ctatggtaga gaccatgcct agaaaggttc tgccaatatg
    60541 ccaaattttt ctctcaataa tggtgcaaag tatagggctt tttgataaaa tacgtcatat
    60601 agagtaatgg aaggattatt atgaaaaaat ttcagttttt tctacttgtt gaagccgttg
    60661 tcttagttat gggattaatg aaaattttat cagatgactg gacaagtttt atttttattc
    60721 tcgctttgat tcttttagcc ttaagatttt ataacaacga tagtcgtcat aattttctgt
    60781 taacgacaag tttgttatta ctatttttaa tctttatgct taacccttac attattgcag
    60841 ccgttgtttt tgcagttttg tatgttctaa tcaatcattt ctcgcaagtt aagaaaaaaa
    60901 atcgttatgc tttgattcaa tttaagaacc atcaattaga tgttaagaca acacgaaacc
    60961 aatggttagg tactgatcag cacgaaagtg acttttatgc ttttgaagat attaatatta
    61021 taagaatctc tgggactgat acaattgatt taacaaatgt tattgtatct gggcaggata
    61081 atgttattat aattcaaaaa gtttttggtg ataccaaagt attggttcct ttagacgttg
    61141 ccgtaaaggc tgatattagt tctgtttacg gtagtgtaca gtactttgat tttgaggagt
    61201 atgatctaag aaatgaatct ataaaattgt cacaagagga agaatattac cttttgaaac
    61261 gtgtcaaatt agttgtaaac actatcgctg ggaaagttga ggttagtcgt aaatgaaaaa
    61321 gcaccactac tttttagctt tcttctatgg aagtgttatt atatttgcca tttgttttgt
    61381 cattattgat agtttgggtg ttaatttggt gcacctctat caaacgtctc gtttgtggct
    61441 gattgaacaa ctgattttct ctattttctt tctatcttta gcggtaacta tccttttatt
    61501 gctaacttgg tttttattag acgataatag taaacgtcaa attaaccata atttacgtcg
    61561 cattttaaat aaccaatcaa taaatgttac cgatgatggt actgaaatat cgaccaatat
    61621 tcaacggctc tctaaaaaaa tgaatttaat gacagcaagt ctacaatcta aggaaaatag
    61681 tcgtatttta aaaagccaag aaattgtgaa acaagaacgt aagcgtattg cacgtgattt
    61741 acacgacaca gttagtcagg atttgttcgc agcttccatg gttttgtcag ggattgcaca
    61801 gaatgtttct cagctagacg ttgatcaggt tggctcgcaa ttattagcag tagaggaaat
    61861 gcttcagcat gcacaaaatg atttgagaat tctgctactt catttgaggc cagttgaatt
    61921 agaaaataag accctatctg aaggattcaa aatgatattg aaagaattga ccgataagag
    61981 cgacattgaa gttgtttatc acgaatcaat tctaacctta cccaaaaaga ttgaagacaa
    62041 tatttttaga attggtcaag aatttatcag taatacttta aagcattcac aagccagtcg
    62101 tctcgaagtg tacctcaatc aaacagagaa tgaattgcag ttaaaaatga ttgataatgg
    62161 tattggattt gatatggatt ctgtctatga tttaagttat ggattaaaaa atattgaaga
    62221 tcgagtagaa gatttagcag gaaatttaca gcttctcagt cagcctggta aaggagttgc
    62281 tatggatata agattacctt tagtaaatca gagtgaggat aaaaatggat aaaataaaaa
    62341 ttgtacttgt tgatgaccat gagatggttc gtctcggttt aaaaagtttt ttaaacttac
    62401 aagctgatgt tgaagtaata ggtgaagcct caaatggttt agaaggcata aaaaaagcac
    62461 tggagttacg tccagacgtt gttgttatgg atttagtaat gcctgaaatg gacggtgtag
    62521 aagcaacctt agcattgttg aaggattggc cagaagctgc tattttagtc ttaacatctt
    62581 acttggacaa tgaaaaaatt taccctgtta ttgaagcagg tgccaaaggc tatatgttaa
    62641 agacctcaag tgcagcagag attcttaatg ccattcgtaa agttgcaagg ggagaacaag
    62701 ctattgaaaa tgaagtagat aagaaaatta aggctcatga taaatgtcca gctttacatg
    62761 agggcttaac agcaagggaa cgtgatatcc ttaatttatt agccaaaggt tatgacaatc
    62821 aaaggattgc tgatgaatta tttatctcgt taaagactgt taaaactcat gtttctaata
    62881 ttttgggaaa actaaatgtg gcagatcgaa ctcaagcggt tgtctatgct ttccaacacc
    62941 atttggtacc acaagatgat gaataagtga tataatagag acactatgtt aatgaaagtg
    63001 gttagtcatg gacgcaaaaa ctaaatataa agcaaaaaaa ataaaggcgg tgttttttga
    63061 tattgatgac acgcttcgtg ttaaagatac aggttatatg ccaccatcga tcttaaaagt
    63121 ttttaaagct ttgaaagata aaggaattgt tgtaggtatt gcttcaggaa gggcacgata
    63181 tggcgttcca aaagaagttc aagatttaaa tgccgattat tgtgtaaaat taaatggagc
    63241 ttatgttaag gacaaggata agaatatcat cttccatcgt cctattcctg ctgaatatgt
    63301 agagcaatat aagaaatggg cagacactgt tggtattaag tacggtttag ctggtcgtca
    63361 tgaggcagtt ttatcagatc gtgatgattt ggtaaatgat gcgattgata ttgtgtattc
    63421 tgatctcgaa gttaatccag atttcaataa agaacatgat atttatcaga tgtggacgtt
    63481 tgaagataaa ggagatagcc ttcatctacc tgaaccgtta gatgaacatc ttcgcttgat
    63541 acgttggcat gatcattctt cagatgttgt gttaaaagga acatcgaagg ctttaggtgt
    63601 ttctaaagtt gttgaacatt tgggcttaaa gccagagaat atcttggttt tcggagatga
    63661 gctcaatgat ttagaactct ttgattatgc tggactagct gtggcgatgg gagtgtcaca
    63721 cccagaagct caaaaaaaag cagattttat cacaaaaaaa gtagaagaag atggcatttt
    63781 gtatgccctg gaggaactag gtttgataga aaaagaatta acatttccac aagtagatat
    63841 tgaaaataca gaaggtcctg tggctgtcat taaaacaaat cacggggata tgactgtaaa
    63901 actcttcccg gatcatgctc ctaaaacggt agcaaacttt attggtttag ctaaacaagg
    63961 ttattatgat ggtattatct tccatcgtat tattccagat tttatgattc agggtggtga
    64021 tccaactggt acaggtatgg gtggtgaatc tatctatggg gaaagttttg aagacgaatt
    64081 ttccgaagaa ttatataatg ttcgcggagc tctttcaatg gctaatgctg gaccaaatac
    64141 gaatggcagc caatttttca tcgtccaaaa tacaaaaatt ccttatgcga aaaaggaatt
    64201 agagcgaggt ggttggccaa caccaattgc tgagttgtat gctggacaag gtggtacacc
    64261 gcatctagat cgtcgccaca gtgtatttgg acaattagta aaccaaagtt cttttgaggt
    64321 acttgatgag attgcggcag tagagactgg ttctcaagac aaaccattgg aagatgttgt
    64381 tattttaacg attgaggtta aagaataaaa tgaagattgg cgataagtta aagggaacag
    64441 tcactggcat taggccttat ggagcctttg tttctcttga ggatggtagg actggactta
    64501 tccatatttc tgaaattaaa acaggttaca ttgataatat ttatgatgtt cttagtgtag
    64561 gtgatgaagt ctatgttcag gttatagatg tagatgaatt tacacaaaaa gcaagtttat
    64621 cgcttcggac ccttgaagaa gaaagacatc atattcagca tcgtcatcga ttttctaaca
    64681 atcgtcttaa gataggtttc aaaccacttg aggaaaattt acctagttgg gttgaggaag
    64741 gtctagccta cttgaataat aaataaacaa aaacctagat ttgattaatg acaagtctag
    64801 gttttaaata tttaaaagta gcagtgaata ttatacttgc taaaaatagc ttgataatcg
    64861 agtagatcct ctgggtgtag tgcagcgatc tcttccattt catattgacg attaagtaat
    64921 cgatatttgt tttgtccaaa ttggtggaaa ggtaagagtt gcacttgacg gatatctagg
    64981 cgatcaaata aacaagcaaa ttcttcagca tcttctaatg aatcgttaaa attaggtatg
    65041 acaggaatac gtagcacaat cgttttaccg ttggcaaaag cataatgaat gtttttgatg
    65101 attgaagcat tcttaaccat cgtcttttct tggtgtttga ggctattgta atgttttaaa
    65161 tcggtgtaaa taaaatcaac gtattggata aggtctatga attgttcatg tctagtataa
    65221 gcagttgttt caatagccgt gtggattccc agtgatttag cacgttttaa aattgccttg
    65281 gcaaattcaa attgtgcaaa tatttcaccg ccagatagcg ttatgccccc accagattct
    65341 tcgtaaaaat caatatcttt taaaacctct tcaataatat catcaaccga tttttcttcg
    65401 cccacgatga cgctttcatt agtaatggca tctctcattg tttctggaac cattttttgc
    65461 gactcgggat tagcacacca aggacatcta agaggacaac cctttaagaa aacggttgtt
    65521 cgaatacctg gaccatcgtg aatactaaaa tgttgaatgt tgaacacaat acctttttca
    65581 tttgtcatat tattttcctc aatttcattt taagtatac
//
LOCUS       DATRSG010000003        59951 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRSG010000003 DATRSG010000000
VERSION     DATRSG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59951
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.003"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(151..2979)
                     /gene="uvrA"
                     /locus_tag="VBD89_000134"
     CDS             complement(151..2979)
                     /gene="uvrA"
                     /locus_tag="VBD89_000134"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO7134695.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDVDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVSIGTPEEVAENPKSYTGYYLKEKLAR"
     gene            complement(3111..3782)
                     /locus_tag="VBD89_000135"
     CDS             complement(3111..3782)
                     /locus_tag="VBD89_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO7134696.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            complement(3807..4751)
                     /locus_tag="VBD89_000136"
     CDS             complement(3807..4751)
                     /locus_tag="VBD89_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7134697.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     KNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            complement(4921..5160)
                     /locus_tag="VBD89_000137"
     CDS             complement(4921..5160)
                     /locus_tag="VBD89_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO7134698.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(5205..5696)
                     /locus_tag="VBD89_000138"
     CDS             complement(5205..5696)
                     /locus_tag="VBD89_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7134699.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            complement(5708..5995)
                     /locus_tag="VBD89_000139"
     CDS             complement(5708..5995)
                     /locus_tag="VBD89_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO7134700.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            7089..8213
                     /gene="mutY"
                     /locus_tag="VBD89_000140"
     CDS             7089..8213
                     /gene="mutY"
                     /locus_tag="VBD89_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO7134701.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            8390..8983
                     /locus_tag="VBD89_000141"
     CDS             8390..8983
                     /locus_tag="VBD89_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134702.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(9029..9343)
                     /gene="trxA"
                     /locus_tag="VBD89_000142"
     CDS             complement(9029..9343)
                     /gene="trxA"
                     /locus_tag="VBD89_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO7134703.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(9424..9924)
                     /locus_tag="VBD89_000143"
     CDS             complement(9424..9924)
                     /locus_tag="VBD89_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7134704.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(9928..12267)
                     /locus_tag="VBD89_000144"
     CDS             complement(9928..12267)
                     /locus_tag="VBD89_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO7134705.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(12352..12894)
                     /locus_tag="VBD89_000145"
     CDS             complement(12352..12894)
                     /locus_tag="VBD89_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO7134706.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(12897..13208)
                     /locus_tag="VBD89_000146"
     CDS             complement(12897..13208)
                     /locus_tag="VBD89_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134707.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            13319..14212
                     /locus_tag="VBD89_000147"
     CDS             13319..14212
                     /locus_tag="VBD89_000147"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO7134708.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            14228..14821
                     /gene="lepB"
                     /locus_tag="VBD89_000148"
     CDS             14228..14821
                     /gene="lepB"
                     /locus_tag="VBD89_000148"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7134709.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGSFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            14950..17370
                     /locus_tag="VBD89_000149"
     CDS             14950..17370
                     /locus_tag="VBD89_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO7134710.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
     gene            17484..17966
                     /locus_tag="VBD89_000150"
     CDS             17484..17966
                     /locus_tag="VBD89_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134711.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(18037..19146)
                     /gene="dinB"
                     /locus_tag="VBD89_000151"
                     /pseudo
     CDS             complement(18037..19146)
                     /gene="dinB"
                     /locus_tag="VBD89_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
     gene            19316..21628
                     /gene="pflB"
                     /locus_tag="VBD89_000152"
     CDS             19316..21628
                     /gene="pflB"
                     /locus_tag="VBD89_000152"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO7134712.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            21729..22102
                     /locus_tag="VBD89_000153"
                     /pseudo
     CDS             21729..22102
                     /locus_tag="VBD89_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(22137..23066)
                     /locus_tag="VBD89_000154"
     CDS             complement(22137..23066)
                     /locus_tag="VBD89_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO7134713.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(23063..23818)
                     /locus_tag="VBD89_000155"
     CDS             complement(23063..23818)
                     /locus_tag="VBD89_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO7134714.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESWGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            23943..24839
                     /locus_tag="VBD89_000156"
     CDS             23943..24839
                     /locus_tag="VBD89_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO7134715.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(24918..25766)
                     /locus_tag="VBD89_000157"
     CDS             complement(24918..25766)
                     /locus_tag="VBD89_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7134716.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAIFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGSTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(25934..26386)
                     /locus_tag="VBD89_000158"
     CDS             complement(25934..26386)
                     /locus_tag="VBD89_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7134717.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFESLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(26404..27606)
                     /locus_tag="VBD89_000159"
     CDS             complement(26404..27606)
                     /locus_tag="VBD89_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7134718.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            27762..28421
                     /locus_tag="VBD89_000160"
     CDS             27762..28421
                     /locus_tag="VBD89_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7134719.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            28445..30730
                     /locus_tag="VBD89_000161"
     CDS             28445..30730
                     /locus_tag="VBD89_000161"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO7134720.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     NWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTITFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            30734..31093
                     /locus_tag="VBD89_000162"
     CDS             30734..31093
                     /locus_tag="VBD89_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134721.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKNFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            31139..32119
                     /locus_tag="VBD89_000163"
     CDS             31139..32119
                     /locus_tag="VBD89_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7134722.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(32204..33952)
                     /gene="cydC"
                     /locus_tag="VBD89_000164"
     CDS             complement(32204..33952)
                     /gene="cydC"
                     /locus_tag="VBD89_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO7134723.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(33945..35663)
                     /gene="cydD"
                     /locus_tag="VBD89_000165"
     CDS             complement(33945..35663)
                     /gene="cydD"
                     /locus_tag="VBD89_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO7134724.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYVLLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(35663..36682)
                     /gene="cydB"
                     /locus_tag="VBD89_000166"
     CDS             complement(35663..36682)
                     /gene="cydB"
                     /locus_tag="VBD89_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO7134725.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(36683..38110)
                     /locus_tag="VBD89_000167"
     CDS             complement(36683..38110)
                     /locus_tag="VBD89_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO7134726.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(38213..39421)
                     /locus_tag="VBD89_000168"
     CDS             complement(38213..39421)
                     /locus_tag="VBD89_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7134727.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(39434..40333)
                     /locus_tag="VBD89_000169"
     CDS             complement(39434..40333)
                     /locus_tag="VBD89_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO7134728.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(40742..41347)
                     /locus_tag="VBD89_000170"
     CDS             complement(40742..41347)
                     /locus_tag="VBD89_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134729.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGVFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            42230..42529
                     /locus_tag="VBD89_000171"
     CDS             42230..42529
                     /locus_tag="VBD89_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO7134730.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQTQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            42689..43879
                     /locus_tag="VBD89_000172"
     CDS             42689..43879
                     /locus_tag="VBD89_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7134731.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(43949..44674)
                     /locus_tag="VBD89_000173"
                     /pseudo
     CDS             complement(43949..44674)
                     /locus_tag="VBD89_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(44801..45388)
                     /locus_tag="VBD89_000174"
     CDS             complement(44801..45388)
                     /locus_tag="VBD89_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO7134732.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(45432..45968)
                     /locus_tag="VBD89_000175"
     CDS             complement(45432..45968)
                     /locus_tag="VBD89_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO7134733.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            46103..47275
                     /locus_tag="VBD89_000176"
     CDS             46103..47275
                     /locus_tag="VBD89_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7134734.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(47319..48101)
                     /locus_tag="VBD89_000177"
     CDS             complement(47319..48101)
                     /locus_tag="VBD89_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO7134735.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            48260..48529
                     /gene="rpsN"
                     /locus_tag="VBD89_000178"
     CDS             48260..48529
                     /gene="rpsN"
                     /locus_tag="VBD89_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO7134736.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
     gene            complement(48890..49915)
                     /locus_tag="VBD89_000179"
     CDS             complement(48890..49915)
                     /locus_tag="VBD89_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO7134737.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(50035..51045)
                     /gene="tsaD"
                     /locus_tag="VBD89_000180"
     CDS             complement(50035..51045)
                     /gene="tsaD"
                     /locus_tag="VBD89_000180"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO7134738.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            complement(51121..51528)
                     /gene="rimI"
                     /locus_tag="VBD89_000181"
     CDS             complement(51121..51528)
                     /gene="rimI"
                     /locus_tag="VBD89_000181"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO7134739.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            complement(51530..52222)
                     /gene="tsaB"
                     /locus_tag="VBD89_000182"
     CDS             complement(51530..52222)
                     /gene="tsaB"
                     /locus_tag="VBD89_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO7134740.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVSEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            52401..52631
                     /locus_tag="VBD89_000183"
     CDS             52401..52631
                     /locus_tag="VBD89_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO7134741.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            52685..54364
                     /locus_tag="VBD89_000184"
     CDS             52685..54364
                     /locus_tag="VBD89_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7134742.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(54522..55031)
                     /locus_tag="VBD89_000185"
     CDS             complement(54522..55031)
                     /locus_tag="VBD89_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7134743.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(55179..56525)
                     /gene="glnA"
                     /locus_tag="VBD89_000186"
     CDS             complement(55179..56525)
                     /gene="glnA"
                     /locus_tag="VBD89_000186"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO7134744.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            complement(56559..56930)
                     /locus_tag="VBD89_000187"
     CDS             complement(56559..56930)
                     /locus_tag="VBD89_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7134745.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            complement(57010..57549)
                     /locus_tag="VBD89_000188"
     CDS             complement(57010..57549)
                     /locus_tag="VBD89_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO7134746.1"
                     /translation="MKTLKKLLSNYKFDIKKFKFGMRTFKTGLSVFLVLLVFHLFGWN
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            complement(57812..59008)
                     /locus_tag="VBD89_000189"
     CDS             complement(57812..59008)
                     /locus_tag="VBD89_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO7134747.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(59143..>59951)
                     /locus_tag="VBD89_000190"
     CDS             complement(59143..>59951)
                     /locus_tag="VBD89_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO7134748.1"
                     /translation="CQSNHSTKKADMSPDKVEQKTKFKNISLSSNKLLAKENTMSVLW
                     YQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSIILDLDETVLDNSPYQAKNIKDG
                     SSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKIYYVSDRTDAQVDATKENLEKE
                     GIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLFGDNLVDFADFSKSSSTDREQL
                     LTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQKQLKERQKMLHSYD"
BASE COUNT        19746 a        11662 c         9584 g        18959 t
ORIGIN      
        1 tagaaactat ttttttcaaa acatcattat ttcctttttt taaaatatct ccccactatt
       61 tttcctaata aagcgttctc catttaatga cttctacgca atgttagctt acaaaaagga
      121 actgagtaat attactcagc tcccaataaa ctatcttgct aacttctctt ttaaataata
      181 tcctgtatag gatttaggat tttcagcaac ttcttctggt gttccgatag atacaatttg
      241 tccgccacca atacctcctt cagggccaag atcaataatg tggtcagcag ttttaataac
      301 atcaaggtta tgctcaataa caagcactgt attaccatca tctacaaaac gatccaaaac
      361 cttaagtaaa cgagcaatat catctgcatg aagccccgtt gttggttcgt ctaatatgta
      421 aagactctta cctgtcgaac gcttatgtaa ttcacttgcc agcttcattc gctgagcttc
      481 tcctcctgat aaggttgtag ctggttgccc caaagtgaca tatcccaaac caacatcctt
      541 aatagtctgt aacttacgag caattttagg aatagctgca aaaaatgtaa ctgcatcatt
      601 cactgtcata tcaagaattt gtgcaatatt tttttctttg taatgtactt ccagagtctc
      661 actattatag cgcgtgccgt ggcatacttc acaaggaacg tagacatctg gtaaaaaatg
      721 catctcaatt ttgatgatac catcgccaga acaagactca caacgtccac ctttgacgtt
      781 aaatgagaag cgtccttttt tataacctcg aatctttgcc tcattagttt gagcaaaaag
      841 atcacgaata tcatcaaaaa cacctgtata ggttgcggga ttagaacgtg gagtccgacc
      901 aattggactt tgatcaatat caatcaatcg atcaacatat tcaatgcctt ctaaagaaac
      961 atatttacct ggtttatctg agttacggtt aagcttctgc gcaacagctt ttttcaaaat
     1021 actattaatt aaggttgatt ttccagaacc tgagacacca gttacggcaa taaatttacc
     1081 gagagggaac ttgacatcta aattttgtag gttgttttct gccgcacctt tgatttctaa
     1141 aaagcgacca ttacctactc ttcgttcaga tggcacagga atcactttct tacctgacaa
     1201 atattgtcct gtaatagatt tagtattttt agctacttgc ttcggtgtac cagaggccac
     1261 aatctcacca ccaaacgcac cagcgccagg tcccacatcg attaaccaat cagctgccat
     1321 cattgtatct tcatcatgtt caacaacaat aagcgtatta cccaaatcac gcattttctt
     1381 aagactatca atcaaacggt cattgtctcg ctgatgaagt ccaatggacg gctcatcaag
     1441 tacataaagg acacctgata aattagaccc aatttgagta gccaaacgaa tacgttgact
     1501 ctcgcccccg gaaagtgttc ctgcagaacg tgacagattt aaataattaa gtccaacatt
     1561 atttaagaaa cttagacggt catggatttc tttgataatt ggtcgagcaa ttaattgttc
     1621 atttgaagat aaacgcaatg tttctaaaag ttctaaatgg tctgctatcg ataaatctga
     1681 gacttgccca atattaagac cttcttctcc cccaactctt acgcaaagtg cttgatcatt
     1741 gagacgatag ccatgacaag tgttacattt tagctcgttc atatattctc gcataacatt
     1801 gcgtgtgtaa tcgctattag tttcatggta acgacgatta atattattga caaccccttc
     1861 aaaaggcaaa tcaatatttc tctcgcctcc aaaatcgtta atataatgga aatggaactc
     1921 tcgttcgcct gaaccatata gggctaggtc ctgctccgcc ttgcttaatt tttcaaatgg
     1981 cgtatccaca tcaacaccaa attgagtcat agcttgctct agcatcgttg gataataatt
     2041 tgatgaaatt ggattccaag gaactaaggc cccttctcta agggtcttac ttctatctgg
     2101 aataaccaaa tctatatcta cttctaactt aattcctaag ccatcacagg tcggacaaga
     2161 accaaaggga gcattaaaag aaaaaaggcg tggctctaat tcaggaacag taaaaccaca
     2221 ttcaggacaa gaataatgct ctgaaaacaa taactcattg ccatccattg tatcaataac
     2281 gacataacca tccgacaaac gtagagccgc ttcaacggaa tcaaataagc gggaacgaat
     2341 gccctcttta ttaattaatc gatctactac aatatcaata ttgtgcatct tactttttga
     2401 taattcaggc acctcagtaa catcgtgaat atcaccatca atacgaacac gaacgtaacc
     2461 atctttttgt attttctcaa atgttgattt gtgttgtcct ttttttcgac gtataattgg
     2521 ggctaaaatt tgcatctttg ttcgctctgg aagcgctagt accttatcca caatttgctc
     2581 tacagaagat gctgtgatgg ctccgtgacc attgatacaa tatggggttc caacacgagc
     2641 ataaagcaaa cgtaaataat cgttaatttc tgttacagtc ccaacagtag agcgaggatt
     2701 cttacttgtt gttttctgat caatagaaat agctggacta aggccatcaa tagaatctac
     2761 atcaggcttt tccatatttc ccaaaaattg gcgcgcgtaa gcagaaagac tttcaacgta
     2821 acgacgttgt ccttctgcat agatagtgtc aaaggccaaa ctagacttac ctgatcctga
     2881 tagtcccgta acgacaacta acttgtctcg tggaatgtcg acacttatat tttttaaatt
     2941 gtgagctctt gctcctctta tcattaattt atcttgcata atgttgtgcc tcagcactcc
     3001 tttttgaata gtgttgactt attatatcaa attttaataa agtcagtatt tttttagttt
     3061 taaatttcat agaaaaaaga gatgaactaa tacgttcata ctctttgtta ttatcttctt
     3121 tgttgtggtg ccactggtgc cattgagctt tgaatattat atttacgttg taaataaaag
     3181 cgcaaaccaa acgatgctaa agcaataata aacaaaacaa cctcaggaag tttaggatta
     3241 atagttgcag gtagaagagg tactactgca aataaggcaa accaaactaa cataactagt
     3301 gccagtactg cgaaagattt aagccagcct ggtctttcag attttggttt acccatatgg
     3361 cgataaatat agtaataagt aacgtacatg gccgcaccac caccaaaacc aagagttaaa
     3421 attgaaataa gccctgtatt gacattagat tgaccaaata gcaacattaa accattcaaa
     3481 gcagttacta agccaagaaa tagtaaggaa gtatctaacc acatcaacca aggattggta
     3541 tttttttgta cagatactct agctttatcc tcaggtcgct cagtgaatga ggctgcccaa
     3601 gtagttggtg ctcctaagag tgagcgtgcg gtaattcctt ttttctgatt atctataatc
     3661 tctgggagat gttcttctaa aatagctttg atttctgcat ctgattttcc atcagctaaa
     3721 agttgattag ttgcaatatg aacaaactct tgattctttt tagttaagtc ttgtaaagtc
     3781 atgtgaactc cttgattaat atactattaa aaccattttt tacgaataaa ataaaataca
     3841 acaaatgaac tcatcaaaaa ggccaaaaga acaacataga taaagccatg tgcgagacca
     3901 ttcaaaggca tccaattatt tttaaagttc atcccatacg ccgaaaaaat aaccgttgga
     3961 atatccaatg tcatagttac tagggctaag gttttcataa tggtattctg attattaccg
     4021 atgattgaag cagtcgtttc tgtcatggca tttaagacat tttcatagat atttgccatt
     4081 tcaatagcct gctgagtttc gatcaatgta tcttcaagca gatcttcatc ttcaatgtat
     4141 ttttttaaac tactggtact actggtcaat tttttgacga ttcgctcatt aaattttaaa
     4201 gaggccttta gatagacaat agatttttcc aattccatca tatcgatcaa ttgctcattt
     4261 cgagttgcac tctctagttg tgcttcaatt ttatcgctct gcctatcgat agtacgcaaa
     4321 gcctgtaagt ataattcggc attgcgataa agcaattgga aaacaaaacg tgtcttcata
     4381 aaggtataaa aatttttgac acgtcgtcta taaaagtgat caaacagcgt caaatgttct
     4441 aggcaagtag taataacagc gttgtcagta acgataatgc ccataggaat agtcatataa
     4501 taacttttgt tattgcgttc ttcataggta ggaacgtcaa caataactaa agtataatca
     4561 tcttcaaccg agatacggga agtttcttcc acatcgaggg gagcacgtaa atcatcaata
     4621 tcaataccaa attgatcagc tacttgttcg gattcttcct gggagggatt aaccagattt
     4681 atccaagcac ctggctcgaa tgtttcaatt tctttaaatt caattgccgt tgaaagaaac
     4741 atttgcttca tggcgattcc tcctttgtta taaaaaatcc ataccttagt attatacagg
     4801 aaaaccattt gttttgcacg gttaatacgg tggtatccta aatttttcta ttatttgact
     4861 cgtaaatacc aataataatg gtaaaaagaa taaaaaaata gccgctaggg ctattttata
     4921 ttaatcttca tttacgtaag gcataagagc cataacacga gcacgtttga ttgctgttgt
     4981 tactttacgt tggtttttag ctgaagttcc tgttacacga cgtggtaaaa tttttccacg
     5041 ttctgaaacg aaacggctaa gaagctctgt atctttgtaa tcaacatatt caatcttgtt
     5101 agctgcgata taatcaactt ttttacggcg tttgaatccg ccacgacgtt gttgagccat
     5161 gtttatttct cctttatagt tttaaattct gtccatctat tatattagaa tggtagatca
     5221 tcatctgaaa tatccattgg atttgagtta ccaaacggac tctcatcacg actaaaatta
     5281 ggtgtttgtt gagaagaagc tgaataacta ttatttgacg gagcattgtt atagccaccg
     5341 ttattataag agttaggtga accaccttct cgtgtagcac gactttccaa taattggaaa
     5401 ctttccgcaa caacttctgt tacatagaca cgttgacctt gttggttttc ataattacgc
     5461 gtttggatac gacctgtaat tccaaccaaa gcaccttttt ttgcccagtt agccaagttt
     5521 tcagcttgtt ggcgccaaat aacacagtta ataaaatcag cctcacgttc gccagattga
     5581 tttttaaaat tacgattaac tgcaagtgaa aaagtggcta ccgcttgatt acttggtgta
     5641 taacgaagtt ctgcatcacg ggtcatgcga cctacaagta ctacattatt aatcataaac
     5701 taccttctta gtctacttta acgatcatgt gacgaagaat gtcaccgttg atttttgaaa
     5761 gacgatcaaa ctcattaaga gctactgcat cttcagcttc aacgttaacg atatggtaaa
     5821 gtccttcagt gaaatcttgg atttcgtatg caagacgacg tttttcccaa tcttttgatt
     5881 caacaacagt tgcaccgttg tcagataaga tagaatcaaa acgtgctacc aaagcgtttt
     5941 tagcttcttc ttcaatgttt ggacgaataa tataaagaat ttcgtattta gccattgata
     6001 tgttcctcct tttggtctaa tgactcatga atttttcatg agtaagtgag gttatgctca
     6061 caagatatta ttatagtaga aataagttaa taatgcaagg tttttatgtt tttatttccc
     6121 tgatatcatt taaagacatt ttttattttt ctttattgaa atatgcctta tatcaaacct
     6181 atgtagccat tttcttttac tgacttatgt tcaatcccca ttctataatt caattaaaaa
     6241 ataaaaaggc gaggtttaca cctcaccttc aacatattat ttaaagaatt ctttattggt
     6301 ttaagttttc gttggagaaa aattcagtgt ctccgatttt atatttttaa atattaaaat
     6361 actatttagg gagtccctct ttatgtttta ttacctttgg taacaccaaa acatacacaa
     6421 acttttattc atatgtgtta ccattttaca tctttcatat tgagatattt gattagcaat
     6481 acctccttga ctaacaaaaa ttactaacac atctaggggt ataccctagt ttatatttgt
     6541 tcatgattac ctcaagcgag catgcgctaa tggtaatgaa ctattaccac attgtctgtc
     6601 ttccttaaat tatataagga cttatgtgtc accgttactg tagcgaccta catctaaacc
     6661 taacataagt gcccctccaa ctatttctag gaaatgaaga aaacttcaaa tccttgtgac
     6721 tgtttgcaac atcaatgcat tgataacaat tttagtgtat cgccattctt atagcgaccc
     6781 atattaaaaa ttaacataag cgctacctcc attaactaaa gttagcttgc ctaccaatct
     6841 ttcttttgga gactagtctc cattcttata cctagagata ttgtaataga tcatggataa
     6901 cctccttgat ttaaacttta tctttataat ttaattatag tgtaaacgct tccttaagtc
     6961 aagaaaaaaa tattgatgca acatttttct tcatcattaa gtacgatcta gtcaacatac
     7021 ctctttgtca aatcctcttt ctttgataca attaatctat gatagattta aaagactatg
     7081 gaattgagat gtggcctgaa gatagaatcg cttcttttcg tcgcacactt ttagggtggt
     7141 atgaccaaga aaaacgtgat ctcccttggc gaagaactac taatccatac tatatttggg
     7201 tttctgaaat catgttgcaa caaacacaag ttaatacggt aatcccttat tataaacgtt
     7261 ttctagagtg gtttcctcaa ataaaagatt tggcagacgc tcctgaagaa caattactaa
     7321 aggcatggga gggactaggc tattattctc gggtgcgtaa tatgcaaaag gctgcacagc
     7381 aagtgatggt tgattttggt ggcatatttc ctcacactta tgatgatatt gcttcattaa
     7441 aagggattgg tccttatact gcaggtgcta tcgccagcat ctcattcaac ttaccagagc
     7501 ctgctgttga tggcaatgtg atgcgtgtca tggctcgttt gtttgaagtt aattatgaca
     7561 tcggtgaccc taaaaatcgt aaaatttttc aagctattat ggaaatacta attgaccctg
     7621 acagacccgg tgattttaac caagcactta tggatctggg tacagacatc gaatccgcta
     7681 agacacctag acctgatgaa agccctattc gtttctttaa tgctgcttat ttgaatggta
     7741 cttatagtaa atatcccata aaaaatccta agaaaaagcc taaaccaatg cgaattcagg
     7801 cttttgtcat tcgtaaccaa aatggtcaat atctactgga gaaaaatact aaagggagac
     7861 ttctaggggg tttttggtca tttcctatta tcgaaacgtc acctctttct caacaattag
     7921 atttatttga tgacaatcaa tccaacccca taatctggca aacccaaaat gaaactttcc
     7981 aaagagaata tcaattaaag ccccaatgga ctgataatca ctttccaaat attaagcaca
     8041 cttttagtca ccagaagtgg acaatagaac tcatagaggg ggtggtcaaa gcaacagatt
     8101 tacctaatgc tcctcaccta aagtgggtag caattgagga cttttctctg tatccctttg
     8161 caacaccaca aaagaaaatg ctagagacct acttaaaaca gaaaaatgct taatcacaag
     8221 tgaactatga cggttcactt tttgtttaca attcctttac gccgctgtaa agttttcctc
     8281 agattatctt ttaataccct ataaatagtg gtctatccct aatgtcaaat acatttgata
     8341 gacccgttag cttatcatcc ttatactaat attatgaaag gagatattta tggaaattgg
     8401 tcagcaaatt attcgctacc gtaaacaaca agcgctctcg caagaagaat tagccgagaa
     8461 agtctatgta agccgtcaga gcatatctaa ctgggaaaat gacaaaactt acccagacat
     8521 ccatagcctc ttactcctaa gtcaaatttt tcaagtgagc ttagaccaac taattaaagg
     8581 agacattgag aaaatgaaat ataccatcac tcaggttgat aaaaagaatt ttgaacgaga
     8641 taccaaagta atggtgacat tgatgatact attaatgatt tcaagttatc cattagtcta
     8701 tttcttagag tggctaggac ttggcatttt tgttttatta tcgataatta ctatgactta
     8761 tgctaatcgt gtggaacgtt ttaagaaaaa atatgatgtg caaacttata aggaaatcct
     8821 agctgtttca agtggaaaat tattagacga aatcgaaaaa cgggaagaaa gagcaaaact
     8881 tccttatcag aaacccctta ttgtcactgt atttttcctc attactgttg ccaccttttt
     8941 tgcaagtcgt tttattttca catggttgtt ccatttaagt tagtagataa taaaataaag
     9001 ctcagcgtga cactgggctt tattttattt aactcaattc agcaataata gctttcagtt
     9061 ggtctttagt atgaacacct gcaacttgtt taaccacttc tccgtctttt ttaaacatca
     9121 atgttggaat tgacataata ccaaactgac gggcagtttc cggattctca tcaacatcca
     9181 ttttcaaaat tttgagttca tcttcatcga tttcttgaga taactgttct aagattggtg
     9241 cttgcatacg acatggacca caccaagttg cccaaaaatc aatgagtact aaaccttctt
     9301 tagtttcttc tacaaatgta gcatctgtta cttctaatgc catctttttt ctccatttca
     9361 tcttaatgat aagcctattg tacaagaatt tagttgtttt tgaaactatt tgctacgaac
     9421 tcgctaaata aataaacaaa gccctaaaat caagccaata aggtaaccta caataacatc
     9481 cttaggatag tgaatacctg ctatcacacg gcaaattgcc aatagaaccg ataatattaa
     9541 acaaactatt ccaaagtaaa cataatatcg taacaaacac atactaatca tcgtagctga
     9601 aaaaacatgt ctgctaggca ttgaccttcc ctcagtatcc ttataaatta aaggtttaat
     9661 attccatttt tcatatggtc gtggaaaatc aaggcgtttc ctaatgtaag acaaagctat
     9721 aaagccaaca gcagggataa ttaatagcct taaccataat tgaaatgtca tcccttgcca
     9781 aagagtaaat atcagaaaag atggatagag tatgtacatc attcctgtga ctatttttag
     9841 taaaaaattt aaaagaatta ttaactgtgg ggattttcta aaaggttgag atagtttggc
     9901 ataaaattgt tcatatgatt ccataattta ccctaacgtt acaattgtgg cgccagatcc
     9961 ccctgcattt tgtggggcat aagcaaaatg cttaacgtgc ttattacgac gaagatattt
    10021 tgtcactccc tcacggataa cgcctgtacc aataccatga atgatatcaa cttgtcccat
    10081 attgttaagc aatgcttgat caataaaatt atctaactct tgcatagctt cttcgtatct
    10141 tttacctcta agatcaagtc gagctcttgg tccagaacta tcagcctttt taaccacatt
    10201 aatctgttta cttttgactt tctgttcttc ttggactcta acaagggtaa attcatcttg
    10261 tgttaatgtc attttgataa ttcccacttg tgcttcccaa cgtccatctt ttaattgact
    10321 agttaaggta cctcgctgtc catagctagt cactataata tcatcaccaa ttctaggagc
    10381 acgagctgct ttaatttttt tagccttatt taagacttta ttttttgata aatcaacttg
    10441 aggtgctaat ttttttattt gtgccttagc atctataatt tcgtgaggtt ttaattggct
    10501 cttatcattg agttttttta agatagtatc actctcattc aaagccatat ctacaatttc
    10561 ttgagcttct tgatagattt tttctaactc tttatcgcgc tcatgtgaaa attcattata
    10621 gagtttctta accgcacgat tgaatttgag gttttcttgt tcaacttctt taatatgatc
    10681 cagtcttcta cgtgtctcaa gtgtctgtgc ctctaactgt tcaataatac ggttaacatc
    10741 tgagtcagaa tctgtcatct gcttagcttg tttaacaata aatggagcta aaccaaggcg
    10801 agaagcaatt tcaaatgcat ttgatcgtcc aggaactcct tgcataaagc gatacgtagg
    10861 gctaagcgtt tcggcatcaa attccatgct cgcattctct acaaaatttg tctcaatccc
    10921 ataagctttt aattctggat agtgcgtggt cgccatcgtt ttgatattac ttaacctaag
    10981 gtgttctaaa atagccatag ccaaactagc accttcttga ggatccgttc ctgctcccag
    11041 ttcatcaaag aggactaaac tattgtggtc agcctcgttt aaaatactga ctatgtgcgt
    11101 catatgacta gaaaaagttg atagactttg ttcaatagat tgctcatcgc caatatctgc
    11161 aaagatattg ttaaatactg caattttact acctttatcc gctaatactg gcaaaccaga
    11221 ctgtcccatt aattgtgcta aaccgagtgt ttttagcata atcgtcttac caccagtatt
    11281 gggaccagtg atgacaattg cagttaaatc ttgatcaaaa tgtaagtcat tagccacagg
    11341 gttacttaac agaggatgac gaacattgat taatgctaac gtgctgtcat tagaaatctc
    11401 aggtatcgtc gccttattat cagacataaa aagatattta gcccttacaa aatcaagatg
    11461 cccaagaatc catgcattat ttctaatagt ggcgacatgg ggtcttaaca agtctgaaaa
    11521 tgcgtgtaaa atacgacttt cttcatgacg ttcgtcagct ctaagctgcg ttatctcttc
    11581 gtttagtgta actacagcac gaggctcaat ataaacagta cttcctgaag aagagatgtc
    11641 atgaaccaca ccagaaatac gattccgata agtatttttt actggtagga cacttcgtcc
    11701 actacgacta gcgattagat tctctgataa aagctctgct ttttccttaa gcatatcctg
    11761 taaaatctga cgaacccgtc gttcactgtt tgttaattga cgacggatac gctctaattc
    11821 tggactcgca aaatgttcta aaaaaccacc atcattgata gcttgaaaag acccttgcag
    11881 attagggaat tgttccaaat tttcaaacaa acgatccaaa gattggaaag aaacattatc
    11941 caaatcagaa taaaagtgaa tcatatccga agaactctgt aaaacttttt tgatagccaa
    12001 aagttcttga atattaagat cagctgaaag ctctaagcgt tttaaactct cagagattga
    12061 acttaggctg actatgccaa atgagtgatg ttcaacaaaa atctgttcca tgtcagaaat
    12121 ttcattaaaa cttttttcta ttttaggagc ctctgtcatc ggctccagct ctgataattc
    12181 ttcttgtgat tgttctgtct tgagataagg taatatcaat tccttaactt tgttaaattc
    12241 taactgttct aaaatcttgt tattcatact cttcattata cctaaattta tcacaaaatg
    12301 acaacgttaa aaaggttgga actcttactc caaccttact aaacattata ttcaaagacc
    12361 taccgcccaa gtccttacca aaaactgtga gaagaatggt aagtcgttaa ttaaaaatct
    12421 aacaaacaga ctattccata acttttcttg aacaaatgac atagggactg ttgccagaat
    12481 agtcaaacac atactcataa ataagagaca aaccagaacg gctaaaaaac cactaataat
    12541 attattttgc aagctatcaa aatgttcaac tggtgctaaa tgtagtaaaa cacctaacaa
    12601 gcgacctact aagtaagata aactgtagat aaaaacaaag gcaacacctg cataataaac
    12661 atgactcaat tgaaatttgc taatatcttt aaaaaagtaa acttctactc cttgtacagg
    12721 attagaatac ggtacccata acgtaatctg actagctaat tcttggtaaa attgactagc
    12781 aatcatcaaa gataccattg acattaacac gtaaaagact tgtaaaaaaa tacctctact
    12841 gtagccaata taaaaatgcc aaataacaat aatcagcaaa agcaatgata gcatgattaa
    12901 tcctcactca ctttttcttt aatgtccgaa atcgttttgg agcgtaactt attgagctca
    12961 tcttccattt tttcaatgtc aatttctcga cttagttgca ctgataaagc attaattgcc
    13021 atgagaatag caatcgtttc attatcagca ttaggtaatt tttctttaat agcttcatac
    13081 ttttctgtag ctacacgctc aatctcttcc ataaaaaggt tgtctttatc tgtcgtcaat
    13141 gtgagaggct tatctccaaa gacaaattta taacgatttt tctgactatt atttattttt
    13201 gtttccatac tatacctctg ctagcattat accgtaaaat agggctttcg taaaattcta
    13261 ccccttatca ttacttgtct attcccttat tcctcttttt tatgatagaa tattgtctat
    13321 gaacacaatc gtcatgcaag cagataaaaa attgcaagaa aagataagaa ccgatttagc
    13381 tcaacatcat ataagtaata acaatcctta tgtggtattt tcagctaaaa tatcaggagc
    13441 tactgttctt ttatatactt ctggaaaact tgtcttccag ggttctaacg cttcacatat
    13501 cgcccaaaag tatggtttta ttgagcaaaa agaaagttgt tcctctgaga gtcaagatat
    13561 tcccataatt ggtactgatg aagtgggaaa tggctcttac tttggtggac tggcagtggt
    13621 agctagtttt gtaactccta aggaccatgc ttacctcaaa aaattaggtg ttggtgattc
    13681 aaaaacactt actgatcaaa aaattaagca aatcgcgcct cttttggaaa aagcaattcc
    13741 acacaaagca ttattgttat cgcctcaaaa atataatcaa gtggttagtc caaataacaa
    13801 acataatgct gtctccgtaa aagttgcttt acacaatcaa gctatcttcc ttcttcttca
    13861 agatggtttt gaaccagaaa aaatcgttat tgatgctttt actagctcca aaaactatca
    13921 aaattatttg aaaaatgaaa agaatcaatt taaacaaacg attactctgg aagaaaaagc
    13981 tgaaaataaa tatctagctg tagcggtcag ttctattatt gcacgtaatc tattcttaga
    14041 aaaccttaac aaattaagcg acgacgtagg atataaactt ccaagtggag ctggtcacca
    14101 atctgataag gtcgctagcc aattactcaa agcttatggt atctcttcgc ttgaacactg
    14161 cgcaaaactt cattttgcca acaccaaaaa agcacaagca ttattaaaat aaatagcagg
    14221 gaaaattatg aaagaattta ttaaagaatg gggtgtcttt atcctcatcc tctcactttt
    14281 tttactatcg cgtatctttt tatggcaatt cgttaaagtt gacggacact ccatggatcc
    14341 aactttagct gacaaggaac agctagtagt tctcaaacaa acaaaaatca atcgattcga
    14401 tattgtagtg gctaacgaag aagaaggcgg ccaaaagaaa aaaattgtta aacgtgtcat
    14461 tggtatgcca ggtgatgtca tcaaatataa aaatgacacc ttaactatta acaataaaaa
    14521 aacagaagaa ccttacctca aggaatatac taaattattt aaaaaggata aattacagga
    14581 aaaatattcg tataacccac ttttccaaga cctagcacaa agctctaccg ctttcaccac
    14641 tgacagcaat ggcagcagcg aatttactac tgtcgtgcct aaaggccact actatcttgt
    14701 tggtgatgac cgaattgtct ctaaagatag tcgtgccgtc ggttccttca aaaaatcaac
    14761 gattgtggga gaggttaaat tccgcttctg gccaattcgt cgttttggaa ctatcaacta
    14821 aaggaaatta aaagttcacc tgacaaaagt tgaaatagca tattatattg aaaataggga
    14881 gtgacaaatc catttaaaat agggatttac aatcactccc tttaactgtt tattaaaaag
    14941 gaaggtacaa tggaagtatt ttttacagga accattgaac gcattatatt tgaaaatgcc
    15001 agcaattttt ttaaaatatt acttctggaa atcgaagata cggatagcga ttttgatgat
    15061 gtagaagtaa ttattactgg tactatggcc gacgttatag agggagaaga atatactttt
    15121 tggggaacac taacccaaca tccaaaatac ggcgagcaac tacaaagtgt acgctatgaa
    15181 agagccaagc ctacttcagg tggccttgtg aaatactttt ctagcgaaca atttaaagga
    15241 attggtaaaa agactgccca acgcatcgta gaactctatg gagataatac cattgataaa
    15301 atccttgaat cccctgaaca gttaagtaca atctcaggac tatcaaaaat taatagagaa
    15361 gcattcatcg ctaaactgaa actcaattat ggaacagagc aagttttagc taaactcgct
    15421 gaatatgggt taagcaatcg tgctgctatt caaatatttg atcactataa agaagaatca
    15481 cttgaagtga taaacgaaaa tccttatcaa cttgttgagg atatccaagg tatcggtttt
    15541 aaaatagctg accaacttgc tgaacaagtc ggtattgaaa gtgattctcc taaacgcttt
    15601 cgtgctgcta ttattcatac tttagttgag tcttctatgg agcaaggaga tacctatatt
    15661 gaggctcgta ctctcttgga aaaaaccatt actctccttg aagaagctcg tcaaatcgaa
    15721 ctagatcctt caattgttgc taaggaattg acaaatttaa ttgcagaaga taaagttcaa
    15781 catattggaa ctaaaatttt tagtaatact ctattttttg ccgaagaagg catcaaaaaa
    15841 aacttacaac gaatactaaa tcagcctctt gataaacaac taaatcacaa agatattgac
    15901 cgtgaaatca gagatattca aaaatcttta aatatccact acgataatat ccaagaaaaa
    15961 gctattagag aagctttact aagtaaggta tttatcttga ctggtggccc aggaacaggt
    16021 aaaacaacag ttataaatgg tattattgaa gcctattccg agttacatca tattgattta
    16081 aacaaaaatg atattcctat tgtacttgct gctcctacag gtcgtgctgc tagacgcatg
    16141 aatgaactta ccggtttacc gagtgctact attcataggc atttgggatt aaacggagat
    16201 agtgactatc aaagtctaga tgactatcta gattgttcct taattatcat tgatgaattt
    16261 tcaatggttg acacttggct tgctaatcaa ttatttgacg ctttggactc gcacacacaa
    16321 gttattattg tcggagatag tgatcaatta ccgtctgtag gtccaggaca agttctagca
    16381 gatcttttaa atataaatgc acttccacac gttaaacttg aaaagatttt tagacaatca
    16441 gaagaatcaa ctattgtgac tttagctaat caaatgagac agggcttttt gccagaggac
    16501 tttacagcta aaaaagccga tcgctcatac tttgaagcat ctgctaatat catcccaaat
    16561 atgatttcta aaattgttca atctgcactt aagagcggta ttgaagctca tgaaattcaa
    16621 attttagctc ctatgtatcg cggccaagca ggtattaata atcttaacct tatcatgcaa
    16681 aatctcctca atccattaaa agataataac caatttactt ttaatgatat aaatttccga
    16741 ataggagata aagtgttaca tttagtcaac gatacagagc ttaatgtttt taatggggat
    16801 attggttaca taacggacct tataccagcc aaatatactg agagcaagca agatgagatt
    16861 tatatgactt ttgacggtca agaagttatc tatcaacgca aagaatggct taaaataaca
    16921 ctagcctatg ctatgagtat tcataaatca cagggttctg aatttcaagt tgttattctg
    16981 ccaataacga gacagagtgg tcgaatgtta cagcgaaacc ttatttatac agctattacg
    17041 agatctaaaa gtaaactaat acttttagga gaaattggtg catttgattt cgctgttaaa
    17101 aacgaggggg caaagcgcaa tacttatctg atagaaagat ttgaaaacaa gcaagaaata
    17161 gctaattctc aaaaaattga agatagctca atagatcaaa agattgacaa cacaataatt
    17221 aatacgtcca ttccaaaaac agcaacacca attgagcaga ctaatctttc aaaaataact
    17281 tatcgcctaa ctgaagaaaa ttatttaaca atcgatccaa tgattggtat taatcaacaa
    17341 gatataagtg caatttttga tagtaaataa attttttcat aaaatcttga aaactcttcc
    17401 aaatatgtta taatatcatt gtattaagaa cgtagttcaa atttaatact tttattaagg
    17461 tttatttaga aaaaggagat attatggttt catatgaaaa agtccgccgc tctctgcgca
    17521 cagctacaat cactatcatt gtattaaata gtttgtcttt agtattcaga ttgtttacag
    17581 gaatttcagt gcagctagcc aaaactgaaa ttaataaagg gaacacaggt aatcttccta
    17641 aagaacatat cgaagcagtc ttgagcgcca caacaccatt tatgctattt gtcactgctt
    17701 taatagtatt agtcaacatt gccattgtta ttttctgtat taaaaattta agagcaatta
    17761 aaagaaatca aatggttaat taccttcctt attaccttgg cttcgccatt actgtaggac
    17821 ttgttatttt gggttttcta actactaaag caccatgggc aatagctata aatatcgtct
    17881 tccaagctat tttcggtctt ctttatttcc atgcttatca aaaagcacaa aaactcaacg
    17941 aacgtgatct tgaagagact aactaaaagt aaaagcacta gaatctagtg cttttttctt
    18001 ttcaaagaga gttaaatata gtcatcgtcc caacttttaa aaatctaaat agatagcttc
    18061 atgttcattt tccagggtcg ttactgtaac acctaagagt cgtatgccaa aaactgactc
    18121 ttctaaggta tcaaatatcg ctttagcaac ttgatcaatg atttgaaaat cttgagtata
    18181 ctcttctaat gtcatacgtt tcgtcaatgt ttcaaaatcc gcatatctaa cttttaaaac
    18241 gattgtctta ccaactttct tatttttctc cagagaagca acaactctct gaacgttctt
    18301 tgaaatctca gctttgatat ctgcttcgtt atatagtaat ttcccatagg ttttctcact
    18361 tcctattgat ttacgaactc tattaggttt taccggggaa gcattaatcc ctcttgcctt
    18421 acgataaagg tcatacccga aacgaccaaa catatcaatt aaagaaattt cagataagga
    18481 aaggaggtcc tcaccagtat aaacccctaa agcatgtaat ttttcgacgg agcgtttacc
    18541 aacaccatga aatttctcta ttggtaaggg cttcaagaaa tcttgagctt gatctggtag
    18601 gatgaaggta agtccttttg gtttctcaaa gtcagaagcc aattttgcca aaaatttatt
    18661 gtaggaaata cccgctgaac aggtcaaatg gacatcattc caaatatcgt attgtatcat
    18721 tttagcaagt ttaacagctg attttattcc cattttattt tcagttacat ctaaataagc
    18781 ttcgtctata ctcatcggtt caaccaaatc cgtttacttt ttaaatatat ctcgtacttc
    18841 cattcctact tggcgatatt tttggtaatt tcccgaaata aagatagctt gagggcatcg
    18901 ttcatacgct tcttttgaag acatagctga atgaactcca aactttcgag cctcataatt
    18961 acaagtagaa acaacccctc tgccgccggt ttttctggga tcagaaccaa taataacagg
    19021 ctttccttta agagagggat tgtcacgctc ttccacagat gcaaaaaaag catccatatc
    19081 aatgtggata attttacgcg acgtatcatt tattaaagga aaaattaaca ttgttctctc
    19141 ccccattctc tctactccat tataacagat tacaacttaa tgatacagat taaaaaaact
    19201 gtattataaa agaatcataa ttttgtcaag aaatatgctt gcgaaaacgt ttactttttc
    19261 ctaaaatgga tatgtaaaca aaattaaatc tgtcacaatg tttaaggaga aaactatggc
    19321 aacagttaaa acaaacacag atatctttga acaagcttgg gaaggcttta aaggtgttga
    19381 ttggaaagaa aaagcaagta ttgctcgctt cgttcaagca aactatgcac cttacgatgg
    19441 agatgaaagc ttcttagctg gagcaactga acgttcactt cacatcaaaa aagtgattga
    19501 agaaacaaaa gctcactatg aagaaactcg cttcccaatg gatactcgtg ttgcttcaat
    19561 ttctgaatta ccagctggtt ttatcgacaa agataacgag cttatctttg gtattcaaaa
    19621 cgacgagctt ttcaaattga atttcatgcc taaaggtggt atccgtatgg cggaaactac
    19681 acttaaagaa aatggttatg aacctgatcc agctgttcac gaaatcttca ctaagtacgc
    19741 tacaacagta aatgatggta tcttccgcgc ttatacttct aacattcgtc gtgctcgcca
    19801 tgcacacact gttactggtc ttccagacgc ttattcacgc ggacgtatca ttggtgtata
    19861 tgctcgtcta gctgtttacg gtgctgacta cttgatgcaa gaaaaagtta atgactggaa
    19921 tgcattgaat gatattgatg aagagtcaat tcgtcttcgt gaagaaatca accttcaata
    19981 ccaagctctt ggtgaagttg taaaacttgg tgacctttac ggcgtagatg ttcgtaaacc
    20041 agccatgaac actaaagaag ccattcaatg ggtgaacatt gctttcatgg ctgtatgtcg
    20101 tgttatcaat ggtgctgcaa catcacttgg acgtgttcct attgtccttg atatctttgc
    20161 agaacgtgac ctcgctcgcg gtacattcac agaatcagaa atccaagaat ttgttgatga
    20221 ttttgtcctt aaactccgta ctgtaaaatt tgctcgtact aaagcttatg atgcccttta
    20281 ctcaggtgac ccaaccttta tcacaacttc aatggcaggt atgggagctg atggacgtca
    20341 tcgtgttacc aaaatggact atcgtttctt aaatacactt gacaatatcg gtaactcacc
    20401 agaaccaaac ttaactgttc tttggtcaga tcaattacca tatgctttcc gtcgctactg
    20461 tatgtcaatg agtcataaac actcttcaat ccaatatgaa ggtgtatcaa caatggctaa
    20521 agaaggctac ggtgaaatgt catgtatttc atgttgtgta tctccacttg atccagaaaa
    20581 cgaagacaaa cgtcacaacc ttcaatattt tggtgctcgt gttaacgtta tgaaagcact
    20641 tcttactggt cttaacggtg gttatgatga tgttcataaa gattacaaag tatttgacat
    20701 cgacccaatt cgtgacgaag tactcaactt tgacactgta aaagctaact ttgaaaaatc
    20761 tcttgactgg ttgactgata cttatgttga tgcgatgaat atcatccact acatgactga
    20821 taaatacaac tatgaagctg tacaaatggc attcttacct tcacatgtac gtgctaatat
    20881 gggatttggt atctgtggtt ttgcaaatac tgtcgattca ttatctgcga ttaaatacgc
    20941 tactgttaaa ccaatccgtg acgaagatgg ttacatctac gactacgaaa cagttggtga
    21001 cttcccacgt tatggtgaag atgatgatcg tgttgactca atcgctgaat ggttacttga
    21061 agccttccat ggacgccttg ctaaacacaa actttataaa gatgctgaag caactgtttc
    21121 tcttcttaca atcacatcaa acgttgctta ctctaaacaa actggtaact caccggtaca
    21181 taaaggagta tatctcaatg aagatggtag tgtaaacctt tctaaagtag aattcttctc
    21241 accaggtgct aacccttcta acaaagctaa aggtggctgg ttacaaaact tgaattcact
    21301 ttctaaactt gatttcgctc atgcaaatga tggtatttca ttgacaactc aagtttctcc
    21361 acgcgctctt ggtaaaacat ttgatgaaca agttgataac ttggtaacag tccttgatgg
    21421 ttactttgaa aacggtggtc aacacgttaa cttgaatgtc atggacctta aagatgtcta
    21481 tgacaagatt atgaatggtg aagatgttat cgttcgtatc tcaggttact gtgtcaatac
    21541 taaatatcta actcctgagc aaaaaactga gttaacacaa cgtgtcttcc atgaagtact
    21601 ttcaatggac gatgcattaa ctaactagtt tgtattcgat gaagtaggtt tgattatatc
    21661 aaacctactt ttatctatat tatcaatagt actaattact tctttatata acctcaagga
    21721 ggggcctaat gttaaatcac aaatttcttc aagttctaaa atatgagggt gtcgtttcga
    21781 taacaagctg gaccgaatta gctcctcacg ttacaaacac ttggaatagc tacctgacta
    21841 ttacagatga ccagcgtata ttagctcctg cagcaggaat gactcatctt gaaatgatct
    21901 caataataat tctaaaatta ttatgactct gggcagccgt gaagtcgagg gtcgtgatgg
    21961 ttatcaaggt acaggcttta gaattgaagg aactgctaag ctattagaag caggaagcga
    22021 ttttgaaatt gttaaagaaa aatacccttt tctgcgtaaa gtattggaag taactcccat
    22081 aaatgttatc caacttttat aaaaaacttc tcctagaaaa ttctaggaga agttttttat
    22141 tcaaagtacg atgttatcgc ctcttgtaca tgtcgtcgtt tcttaatcca aagtggacgg
    22201 tcatcatagg aaaaggttct gtttgtcaaa aaaatagctg ctgcctgctt ttgataattt
    22261 agtgcaataa aaggaccagt ataaccagta tggtttatcc aatccttatc tatatcccaa
    22321 gctaatgatc ttgatttact caaactataa ttattccaca aaggcttcgc aaaatcatct
    22381 tttaaataat gaatagaaaa tctttgtaaa tcatcaatag ttgaaaataa acctgcagaa
    22441 ccagtatgct ttcctaatac cttagcttta ggatcgtgta caataccatc gttaataccg
    22501 gctacagttg gaacagcttc tactctagga ccaaaagagg tttctttcat ctgaaaagga
    22561 gtgaaaatat aacgtttgaa aagtttatcc aaactatccc ctaatacctc ttctaacata
    22621 aaaccaagta aaagaaaatt aatatcagtg tatttaaacg atttatcttc caaaaccttg
    22681 atatgattaa tggcatcttt cagctgagta gcattcaact gatcacgatt tggaataaaa
    22741 gggtcaattc cactgctatg tgtagctaat tgccttacag ttaacgtctg atgatgaaac
    22801 gtagggtaat aataccttaa tggcttatca atatcaatag tcccttgata aactaacttt
    22861 gctagtaaag ttcccacgcc gacaactttt gatacactgg ctagatcata gaccaatccc
    22921 gctttaactt tctcattgcc atcaattgtc cccaagtaat gttcgtgcca ctctccattt
    22981 tcataaatag ctaagcttgc accatgataa agattatctt tgatttgctc cttaatgagt
    23041 tctaaaacgg tattagtttt aatcattctt ttgaaatcca aatctcaata ttactagtgt
    23101 cagagatgac taaaatcttt tctttagaat caaggtaaac atcaagtccc catgattcaa
    23161 aagttgattt caagtcattt aaattagtat cttcattaac acagcattct agtatctcaa
    23221 tatcccaagt ttcatttgga gcaatttgca aatcttgtcc ctgtgcttct ttgaaagata
    23281 aattaattgg gaacttccca gcaaatactt tatcgtaaaa agcttcagct ttcacggcat
    23341 caggtacatt caaactaata gattggaaag caaagtttga tagacctgta gtgtcatctt
    23401 ttttttctaa ctctgggcgc tcaattgcca ctaattgtga caaatcatct tcagcatgaa
    23461 gtaagaaaag gtctccctcg ggcgaaactg tttcataggc ataaccattt tgaccttgat
    23521 atacttgaat ggcttgcgca ccatttgcca aaagcttttc aatatctttt gcatcttgag
    23581 acttaacaat aatctttgct aacttcttag taccattcac cgcacgagta cgataagttg
    23641 gggattcctc aataataaag ctagcctctt tgttctgcca tgctgagaaa actgcgattg
    23701 cattttcttc acttatcaat ttaaatccaa gagactcctg ataaaatgca atattcaagt
    23761 ctctattatt aacacgtaat actgggtgtt taaatgtcaa tgaatgaaat aacgtcataa
    23821 attcctcctt gttcagtcta gtctatttta gataatttta gataatttag caagaatctt
    23881 tgtgctctca ataccataaa gtcaaattga cagtttcact atttttttgt agactggata
    23941 ttatgaatga aacactacta ttacatggga ttcagcttat tctgattatc gccatgatta
    24001 tcacttttta tcagattgtt agacatatta ggtctcaaaa aataaatcct ttcaaacgat
    24061 ttttcactgg tttgtggatc ggttttgtaa ctgatgctct cgatactctt ggtatcggct
    24121 cttttgcaac aacgaccaca ttttttaaac taactaagct tgttgaagac gatcgtaaga
    24181 tacccgcaac aatgacagct gcacacgtac ttccagtttt gttgcagtca ctctgtttta
    24241 tttttgttgt aaaagtagag gctttaacac taataacaat ggctggtgca gcttttattg
    24301 gggcttttgt tggagctaaa atgactaaaa attggcacgc tcctactgtt caacgtattc
    24361 taggaacact gctcatcaca gctgctatta ttatgctcta tcgtatgatc actaaccctg
    24421 gcgctggtat ttcagattca gtgcatggtc tacacggcat ttggttattt gttggtatcg
    24481 tcttcaattt tattattggt gtattaatga ctatgggact aggtaattat gctcccgaac
    24541 ttatcttctt ctccctaatg ggactgagtc ctgcagttgc tatgcctgtc atgatgctag
    24601 atgcagctat gataatgaca gcttctagta cacaatttat caagagtggt cgtgttaatt
    24661 ggaacggctt tgccggtctg gttactgggg gaatattagg cgttattgtc gctgttctct
    24721 tcttaactaa ccttgatctt aacagcttga aaacactggt tgtaggtatt gttttattta
    24781 ctggagcaat gttaatacgt tcttccttta aaaaaactat gcccaaaagt catcattaaa
    24841 cgaaatcagc tagcaagact ggctgatttt tatatttaaa acaaaaaaag accggtaaac
    24901 cgatctttct ttaactatta aagatataat agtttgaaca aggctacagc gagtatactt
    24961 gctacgattg gtgctaaaac tggcacccat gcataccacc attttgaatc ttctttagct
    25021 tgtccaagaa tttgttttgg taataagcgg tgaacaatac gtggaccaag gtcacgcgca
    25081 gggttcaatg caggtccagt cgatccacct aaagaagcaa ctagagtcat taccaagaaa
    25141 ccaataccta agtgagctac agcaagtgaa ccagttacgt atggtgaaat ccttgtcgct
    25201 gctgtcgttt ggtcataacc agcttgaaca agtttaccaa caagttctac accaaaatag
    25261 ttcttcgtta aagccaaagc tccaaagaaa agaacaaaag aaccaacgaa ttcatttaag
    25321 aaaccattaa tgtaagccgc tttacgactt gatttctgtc catcatctaa tgcagaaata
    25381 gttgaaaatg atccaagaac atgatttgga ttttctgtct taacaaagta aggttgataa
    25441 accataacga caactaactg tccaaatatt gctcctaaaa tttgtgcaag aatgtattgt
    25501 ccaacgtgtg cccaagggaa aagacctgaa aatgccaatc caagtgtgaa agctgggtta
    25561 atatggtttc cagagacatt accaaacata agcgctggca tcataacacc taaaccataa
    25621 ccaattgcaa taataatcca acctgaatta tttccttttg tgccttttaa gtcaacgtta
    25681 gctacagccc catttcctaa gatgataaga aaagcggttg cgataaattc tgtgatatac
    25741 ttcacagtcc aagtgatttc gatcaatcct tgtttcctcc ccaaaaaata tttacaacat
    25801 taaccattct accaagtaag cttaatattg taaagcttat cacaaaataa aattaataaa
    25861 atttttaacc aaatatattt gataaaaaat agagtactag gttcggacta cctagtactc
    25921 tattttattt agtttataag gttttcttac tatcacgaac gactagcata tcaactttcg
    25981 aatgacgtaa aatgtattct gaagttgagc cgatcaatag gctttcaaaa gtgtttaatc
    26041 ctgttgcgcc taccataatt aaatctgctt tttcccttat ggggatatcc tttgccaaaa
    26101 gagtcttagg atttccgatt tcagtaattt ttttaatctt tgtaaggcct tttgaatgtg
    26161 ctaatttctc aaaataggct agcacatctg ctgactcttg ttcttgccta tcaaaaacat
    26221 agtcactaaa gactccctca ctttggtaag cgtgagcatc tataacatgc gttagcagta
    26281 attcagcatc attacgaaga gcaacattta tccctttttc taccgccaat tctgactcgt
    26341 aagacccatc aatagcaata agaatacgct catatttttg tgtcatagct taattcctcc
    26401 cttttatata gttttagttt caacttgtct agatgatttt aagaaatata gaataccaca
    26461 aacaacagga ataatcgctg ctatccagaa taattctcta aaggattgca caccagctcc
    26521 aagaaaacca tctttaacaa gtcccaaaat gtaaggtcca gcacctaacc ctaaatcaag
    26581 acctatcatg taagttgaca tggctgtatt gaaacgatgt tcctcaacac ctttgattga
    26641 tgctgcttgg ccacaagaca taaaggtgcc ataaccaaaa ccaatcaaag cacctgatag
    26701 aaggtaggta acacttccca tagcactccc taataaagca agtcccaaag ttaagaacag
    26761 ataacttgga taaagcaccc acttatctcc cttagcgtcc attaatcttc ccatagatgg
    26821 tctggttaaa gtgatgacaa gtgcataaac aataaagaaa taagctccta ctgtcatcaa
    26881 attaatttct gttgtatata atttttggaa acctaacacg gaagcatagg agatacccat
    26941 caaaaatgca ataattgtga taaaaattgc ttttttctca atgaaactat caatagtcca
    27001 tgattttgat ttagctaact gttctggatt taaagtaata tttttgactg ggaaaacaaa
    27061 tgctcccaac actacaatcg caattaaaat actacataat acaataacca ttttaaagtt
    27121 aatatgaagg ttgtctagca taaatgttcc tacaaaagga ccaatagctg cggctaaact
    27181 tgttgataga ccgtaaaagt taatcccctc acctctttta tcagctggta tataggctgt
    27241 tacaatagta ttagttgctg ttgacacgac gccataacca aaaccattta ggaaacgaac
    27301 taaatacatt actccgatac ttggcatata aaaataagct aaagttgtta gtaagtaaaa
    27361 aatagcccct ccacgtaaaa ctaacttacg tcctagaact tctaattgct taccaaatat
    27421 aagacgagca atcaaagtcc ctacaatata aatccccgtt gctaatcctg cttggctagt
    27481 gctaacacct aactctttag tcgcaataaa agctatgata acggtaaaca aataatagac
    27541 catataaaca ataaagttta aaatagtaat gcctataaaa tgtttgttga ataatttgtc
    27601 ttccaatgca aatggctcct tttttcaatt tcattttcat aaacatttta caaaaatatt
    27661 agaagatacg atatttcaat ggtataatat aagtgctagt atcagactgc attaacaaat
    27721 gttaaggtgg tctatcaaac ctaaaacatt tggaggaagt catgaatcat caaattttac
    27781 aaaattacat taatagtcat aatcttccaa ttatcgaaaa agactaccat aaatacctta
    27841 catttgaaag tttagaagaa gactatactt atatcctaaa agatggtatt gttaaacaaa
    27901 gtgttctctc taaatatggt actgagttca atctgcgtta tgtgacaggc ttagaaatca
    27961 cttctatcct aaatacagac tactcccaac acatgggaga accttacaac gttcgaatcg
    28021 aatccgaaac tgcccacttt tacaaagtac gtcgctctac cttcctaaaa gatatcaata
    28081 atgatattga actgcaagga tatgtgaaag atttctatca taaccgcctt gaaaaatcta
    28141 tgaaaaaaat gcaatgtatg ctcactaatg gtcgaattgg tgctatctcc acacaattat
    28201 atgacctcag taagatgttt ggtgaagaac gagataacgg tgatatctat attaattttg
    28261 ttatcacgaa cgaagaactt ggtaaatttt gcggaatatc cacaggaagt agcgtgagcc
    28321 gcattttgaa acaactaaaa gacgatcata ttattcgtat tgaaaaacaa catatcatta
    28381 ttacaaatgt tgagaaatta aaagatcata ttgtatttta atataacaat agaaaggata
    28441 tattatgcgt tataatcaat tcagctacat tccaactaaa cctaacgaag cttttgaaga
    28501 gctcaaagga ctaggttttc cattaaacaa aaagaattct gataaagcta atttggaagc
    28561 ttttctcaga cattcttttt taaatcaaac tgatactgat tacgctctta gtctccttat
    28621 cgttgatgca aaaaccgatg ctctaacctt ttttaaatca aatagtgact taacactaga
    28681 aaatttacaa tggatttatt tacagttatt aggctttatc ccttttgtag actttaaaga
    28741 ccctaaagca ttcttacaag atattaactt cccagtctca tatgacaata tttttcaaag
    28801 tctacatcac ttactcgcct gtcgtggaaa atctggcaat acattaatcg accaattagt
    28861 tgctgatggt ttacttcatg cagataatca ctaccatttt ttcaatggga agtctctggc
    28921 cactttcaat actaaccaat tgattcgcga agttgtctat gttgaaacat ccttagatac
    28981 tatgtctagt ggtgaacatg atttagtaaa agttaacatt atcagaccca ctaccgagca
    29041 tactatccct acgatgatga cagctagccc ctatcatcaa ggtatcaatg atcctgccgc
    29101 agaccaaaaa acataccaaa tggagggtgc gctagcagtt aaacaaccta aacacataca
    29161 agttgacaca aaaccattta aagaagaagt aaaacatcct tcaaaattac ccatcagccc
    29221 tgcaactgaa agcttcacac acattgacag ttatagtctc aatgactatt ttctttctcg
    29281 gggttttgct aatatatacg tttcaggtgt gggtactgct ggctctacgg gtttcatgac
    29341 cagtggggat taccaacaaa tacaaagctt taaagcagtc attgattggt taaatggtaa
    29401 ggttactgca ttcacaagtc ataaacgaga taaacaagtc aaggctaatt ggtcaaatgg
    29461 ccttgtagca accacaggta aatcttatct tggtaccatg tcaactggtt tagcaacaac
    29521 tggcgttgag gggctgaaag tcattatcgc tgaagccgca atctccacat ggtatgatta
    29581 ttatcgagaa aatgggcttg tgtgtagtcc aggcggctac cccggtgaag atttagacgt
    29641 tttaacagaa ttaacatact cacgaaacct cttagctggt gattacatca aaaacaacga
    29701 ttgctatcaa gcattgttaa atgaacaatc aaaagcaatt gaccgtcaaa gtggggatta
    29761 caaccaatac tggcatgacc gtaattacct aactcacgtc aataatgtca aaagtcgagt
    29821 agtttacact catggactac aggattggaa tgttaagcca agacatgtct ataaagtttt
    29881 caatgcattg cctcaaacca tcaaaaaaca ccttttttta catcaaggtc aacatgtgta
    29941 tatgcataat tggcagtcga ttgattttcg tgaaagcatg aatgccttac taagccaaga
    30001 actacttggc attgacaatc atttccaatt agaagaggtc atttggcaag ataatactac
    30061 tgagcaaact tggcaagttt tagatgcttt cggaggaaac catcaagagc aaattggttt
    30121 aggtgatagt aaaaaactta ttgataacca ttatgacaaa gaagcctttg atacttattg
    30181 taaagacttc aatgtgttca aaaatgatct tttcaaggga aataataaaa ccaatcaaat
    30241 cactattaat cttcctctaa agaaaaatta tctcctgaat ggacagtgca aactccatct
    30301 acgtgttaaa actagtgaca aaaaggccat tttatcagcc caaatcttag actatggtcc
    30361 taaaaaacga ttcaaagata caccaaccat cacattctta aacagccttg ataatggtaa
    30421 aaattttgcc agagaagctt tacgtgaact cccgtttact aaagatcatt atcgtgtcat
    30481 cagtaaaggt gtcttgaacc ttcaaaatcg tacagactta cttacaattg aggctatcga
    30541 gccagaacaa tggtttgata tcgagtttag cctccaacca agtatatatc aattgagtaa
    30601 aggtgataat ctaaggatta tcctttatac aactgatttt gaacatacca ttcgagataa
    30661 tgctagttac tctataacag tagatttaag tcaatcttat ttaactatcc caactaatca
    30721 aggaaattaa cttatgaaac ttctcactaa agaacggttt gatgattctc aacacttttg
    30781 gtaccagatc aatttattac aagagagtaa cttcggagca gtttttgacc atgataataa
    30841 aaacattcca caggttgttg caactattgt tgatgattta caaggttccg gaagttcgaa
    30901 ttatttctgg tattttggca atactactga tacttccatc cttatgattg ctcatttaaa
    30961 tcgaaaattc tatattcagg ttaatttaaa gaactttgac tttgcactca atttaatagc
    31021 tataaataat tggaagagtc tcctccaaac tcaactcgaa gctctaaacg ataccctagc
    31081 aatatttcaa taaataaggt agaatggagt gacaaagcaa cgcgagggag actgattaat
    31141 gtcatcttat tggaataact atcctgaact taaaaaaaat attgatgaaa ccaatcaact
    31201 aattcaagaa agaatacagg tcagaaataa agatattgaa gcggcgctaa gccaactcac
    31261 agctgcggga ggaaaacagc tcagaccagc attcttttac cttttttctc aacttggtaa
    31321 taaggagaat caagatactc agcaactaaa gaaaatcgct gcttctttag aaatccttca
    31381 cgttgctaca ttaatccatg atgatgtcat tgatgactca ccactaagac gtggaaatat
    31441 gaccattcaa agcaagtttg gcaaagacat cgcagtttac actggggatt tacttttcac
    31501 agtctttttc gatcttattt tagaatctat ggctgataca ccatttatga ggattaatgc
    31561 aaaatctatg cgtaaaattc tcatgggaga attggaccag atgcaccttc gttacaatca
    31621 acaacaaggt atccatcact atttacgtgc gatttcaggt aagacagccg aactctttaa
    31681 attagctagc aaagaaggag cttactttgg tggtgcagag aaggaggttg ttcgtctagc
    31741 aggccatatc ggatttaaca ttggtatgac attccaaatt ttggatgata tcctggatta
    31801 tactgcagat aaaaaaacat ttaacaagcc tgtcttagag gatttagcac aaggcattta
    31861 cagccttcct ctacttcttg ccattgaaga aaatcctgat attttcaaac ctattttaga
    31921 taaaaaaaca gatatggcta ctgaagacat ggaaaaaatt gcttatctcg tcgtttccca
    31981 tagaggtgtt gacaaagctc gccatctagc tcgtaaattt actgaaaaag ctattagtga
    32041 cataaataag ctaccccaga gctctgcaaa aaaacagttg ctacaattaa ctaattacct
    32101 tttaaaacgc aaaatttaaa taataaaaaa acattccaca atgctagaaa agcagttagg
    32161 gaatgttttt ttattatcat ttatttatcg cacctatcaa tcatcataga tcaccatcat
    32221 cagcggcttt cagctgacgg taacgttggc tactttgaga caattcttga ggagaacctt
    32281 ccaactctaa ttgcccattt tctataaata agatacgatc agcatgttca atacctttta
    32341 agtgatgtgt aatccaaact aaggtcttac cttccaattc tttcataaat acccttagta
    32401 aggcttgttc agtaatagga tcaagtccaa cagttggctc atctaagata acaattggga
    32461 catcttttag taagattcta gccaaagcaa ttctatgcct ttcgccacct gaaaacctaa
    32521 gtccagcttc atcaaccatt gtatagagac catctgataa atcagtgacc atctctttca
    32581 atccaactcg ttcaagaact ttccatacat cttcttcact agcatcttgg tttccaatgc
    32641 gaatgttatt tagcagggtt gtattaaaaa ggtagggcgc ttgttgtatc actccaatat
    32701 agttagaaat gcaatcacca actattgaaa catcagcacc gcctagggta atctcccctt
    32761 gacttgcttt caagtcgcca cgaagtagac tggctaaggt actcttgcca gaaccactac
    32821 gccctaaaat agcaattttt tctccttctt taatatccaa atctaaatga tgcaaaaccc
    32881 atttctcttg tggcttatac tggaaactta aattcttgac ggaaaaatcg tatggcttat
    32941 taggcaactg attttgagtt gtttcaaaat acgtttctga taattcatta agacgatgaa
    33001 ttgaatcaga atatttattt gtttcttgag cagcagcaga caaaccagca aatgcctctg
    33061 acaagggaaa tactgttaga acaaatgctg caatccagtt agcttcgcca cctctgtgac
    33121 caataaactg attactagcc caaataataa ccaaaatagc tagaaagcca aaaactaatt
    33181 ccacaataag agctctacga ttatcaaagg aacgaatttt tttctgaaca gccatgagtt
    33241 cactttccga acgctcatgc aaggcgacgt actcttgacc acgttgacta aaaatccagt
    33301 ctgaaatacc taagacatta tccgtcaaat cagtataaag atgatttttt aattcttttt
    33361 cacgagtctg acgagcacca ttagctaaaa tcgaccaaag tggaaaaaga aaaatcataa
    33421 tagctaaata aagcaacatc attaaagcaa accatagaga aaagaagcct gttgcaataa
    33481 taataaagct atataatatc cacgctataa tcgttggaaa tattgtccta agatacaaat
    33541 tttgcaaata attaatatcc tccgctaaaa gccccataac atctcctaaa cgaaaatcac
    33601 gcttcataaa gatagcattg ctttctaagg aatggtaaag cttcaagcgc agctgtgaag
    33661 tcattcgtaa tacccaattg tgactcgtta ggcgttctat ataacgaaaa acaggacgtc
    33721 caatgccaaa agcacgtgtt agtacaattg gaacatatac tagaagaata ttactcggta
    33781 gtgaagctga cttactaatc agatagcccg aattaaacat aagagcactg gcagaaaaga
    33841 aagtcataaa accaaggaaa agagcaatta ctaaggaaac tttatattgt ttgaaaaatg
    33901 gttttatcca ttgatctgtc tttaattctt taaacaaagg gattttaaac atcatttcct
    33961 cccatagcat gcttcagttg atagagatat ccttttttag ctaataattc ctgataagag
    34021 cctacttctg caactctccc cttctccata acgactataa catccatttg gttcaaccag
    34081 tgaagacgat gagttgcaaa gataactaaa cgatctgaca tcagtggcaa cattttctct
    34141 tttaattcta actctgtttc aatatctaaa tgagcagttg gttcatcaaa aaccataata
    34201 cgacgatttt gatcaagaaa agcacgagcc agagctatac gctgggcttg tcctccacta
    34261 agcggacgcg ctccattacc aatacgcgtt tctaaaccgt caggtaactc gcttagcaag
    34321 ctatctaaac ctaccatatg aatcgctctt accacctctt cgtcactagc attgggagta
    34381 tagaaggtaa tattatcacg taatgacatt tcaaaaacat aaggagtttg aggaatatag
    34441 atcatctgtt tatgccaatc ttcttgatca aggttagtca cttctctacc atccactttt
    34501 aaagaaccat tatcaggccc taaaaaacca ctcagaaggt taattaagct agacttacct
    34561 gaaccactaa ctcctactaa agcaaccttt tgatgtcctc taaatcgtaa ggttacatcc
    34621 tcaacaacac gtttttggtc gtacgcaata gcaatgtttt ctaaatccaa acgactctct
    34681 ttagaccaat catctatgac taattgctca cctttaatac cagttttgtt tagaactttt
    34741 tgaatcgctt gaaatgcatt tttcccgtct aacgttgcat gataatcact agaaaaatct
    34801 cttacgggta aaaagtattc aggggataaa atcaaaattg ttaaagccgg taaaaggtaa
    34861 atctgctcat ttaataatcg taaacctaga aaaacagcaa cgatagcaat ggataaagtt
    34921 gtaaaaaagt ctaaagcaaa cgttgataaa ataccaattt tcaaagtact cattgttgct
    34981 ttacgaaaag attcactagt ttgataaatg cttttaccat aacgtttact aagaccaaaa
    35041 tatttgagag tatcaatgcc tcgtaatgaa tctaaaaaat gattagaaag tacttgatag
    35101 gattcatatt gtttatctgc tttagcttga gccgcatagc ctaaaataat cataaagatg
    35161 ataattaaag gaaaaacgat aagtaaaatt gcacctgatt caatatctaa atagaaaata
    35221 taagctagaa taatccatgg gataatagac atgttaatca tcttattcaa gactaaacgt
    35281 aaataatttt caactaaaga aacgccatct aatgccattg taacaacatt accagttcct
    35341 tgttcttgaa cgactttagg acctagatca aataacttat ctaataattg acgtctcaaa
    35401 agactagatt gtgctgttga aaagtcatca agttttctat ttttgatgta atcaataaca
    35461 tgacggccta aatatgagac cataaataac aaaacataaa ccgtttgaga agaaagtttt
    35521 tgcccttccc acaaaccagt tatagaaaga ctcaaataat aagcttgtcc gataataaag
    35581 attgcttgaa gaacatcaag tcccgcaagc aatcctaaca acttatgaat acctgacaag
    35641 cgcataaccg ctttatctag cattattttt ctcctgtcac aatcactggc aaggtaatac
    35701 gttttctaaa gatataatat gcccatgctg tataagctag aacaaaggga actagagtaa
    35761 ttgcgacaat cgacattact tttaaagtat atggcgttga ggatgcattt tgaatcagca
    35821 aatcatattt agggctaatt gatgaaatca taactcgtgg gaatagacct tggaacaaca
    35881 gaacaaccac tgaaacaaga ctgagaccac tcgctaaaaa tgctgtcatc tctgctcctt
    35941 taaaagttga agcatgcgct aatacagcta atacaacaat aaccaaaacc ataattgtcg
    36001 taactatcgg atgattgcta aagaaatctg tcttgaaata caagagaact gcaaacaagg
    36061 ctaaaccaag atagagtacc caataaagca actgagcata attacgggca cgctctctaa
    36121 ttggcccttc tgtcttcaat gcaatgtaat ttaaaccatg aagataggct agcaatacca
    36181 ttgcaactcc acccaccaac gagaatatat taaagtaatc actgaattga gctgataagt
    36241 taccactggc atcaagtggc ataccttgaa tcaaacttat aaacataatc ccaaagaaga
    36301 atggaacaat agcggaacca attgttaagg tccagttcca aaactgcttt ttctctgcag
    36361 gaactttatg tctaaattcg aaggaaacac cacgaataat taatccaaag agaatcgtaa
    36421 ggagaatcaa atagtaacca ctaaatagtg aagcatacca gtatggaaaa gaagcaaaca
    36481 ttgctccacc accggtaaga agccaaactt cattaccatc ccaaactggg ccaatggttt
    36541 ctactacttg atctttttca tgctcattat gtgttaaagt ttgaacagcc atcccaaccc
    36601 caaagtcgaa tccttcaagg aagaagaatc ctgaaaatag gaggccgatt aggaaaaacc
    36661 aaaagaattg taaagcactc atttagtatg ctcctttctc aaagggatca attgaagtat
    36721 atgcaccatg atgtccgtgt tcatcttcat actctggccc tttcttcaat tctcttattg
    36781 ttaggcttat catcatcacc gctaaaccac caaacaacaa gaaatacaca gtattggaaa
    36841 caaataatga tgcaactgaa acgtttggag agacagagtc cttaatcttg aaaagaccat
    36901 agacagtcca agggtatcgt ccttgttctg tcacaatcca accaaatgta ttggctaaga
    36961 atggtgcaaa ggttgttaaa gcaacaatcc aaagcatcca tttgtgttta taaagaattg
    37021 gttttttctt acgagttaaa aagagtccta aaacagaaac acccataata gctgttccca
    37081 tcgccgccat tgtacggaaa ccatagaaaa gtaggttaac cattggatag tagttatctt
    37141 taccatactt agcaaccaat tctttattag ctgtatccat acctttcaca gaaccagatg
    37201 gtttcccgta agataaaata ctcaacatat aaggaatctt aatcccaaaa acttgtttgt
    37261 gttctgcttc atttgcccaa gcaactacac tccaagctgc aggatcaccc gaatcttcat
    37321 aatcaccttc catagcagca aatttcattg gttgatcgtg aatcaaggct ttcatttgta
    37381 aatccccaac tcccataact gaaattgaac caagaagtgc tactactaaa ccaatacgaa
    37441 ccgatttctt ataaagttca accgttgtat ctttcagctg ttctgatttt aagaggcgga
    37501 aggctgacat accagcaacc actgtaccac ccatcgtaat ggcaccaaaa ataacatggg
    37561 taaattcata gaagaactgt ggatttttta caagtgcaag aaaatcagtc atttgcgctc
    37621 taccgtttac aacttcataa ccaacaggat gttgcatgaa gctattagca gtaagaatcc
    37681 acatcgctga cattaatgag ccaaaaacca ctagccaaat gaatgtcaca tgtaattttt
    37741 tactaatttt tttgttatcc catgtaaaca tccataaacc aaggaaagta gattccataa
    37801 agaaagctaa taaagcttca atagccaatg gagctccaaa aatatcacct acgaaacgtg
    37861 aatagtctga ccagttcatt ccaaattgga actcttgaat gatacctgtc acaacaccaa
    37921 ccgcaaaact aaggagcatg atattacccc aaaattttgt caatttttta tactcttcat
    37981 tttttgtgat aacatacatt gtctccataa tagcaaccac taggcatgta ccaattgtaa
    38041 atggtacaaa gaaaaagtgg aaaactgtag tcatcgcaaa ttgaaaacga gctaaagttt
    38101 caattgtcat acaaaacctc ttattttgtt ttaatcattt cttttataga aatatctgca
    38161 agagctagtt tcctagcttc gtaacaattg gtaaataact tactttaata tcttaatgat
    38221 ataaatcaaa acgtccctta gctagaagtt ctttaagtcc acccatatca acaagtgatt
    38281 tgttcatgat aatcttcttc ataaccgacg cagggtattt tttaatttta gtttttccaa
    38341 caacaccaag tccatgcgta tttcctactg aagcaactgt gccttgcgga ctatacgaaa
    38401 agtcttctgt agcttcacct ttgatttgat gtaaaaggtt tttagcaaca tgagctccca
    38461 tacgagttgc aatttgagct gttgttggga atgggcgacc agactcagtg tccataaatg
    38521 cagacacatc accgataaca taaacattat cgtattttgg atcacgcaaa tcactgttga
    38581 ccattacacg tccacgacgt tgatcaaatc ctgattctcc cataaccgga cttccactaa
    38641 cacctgttgt ccaaatgata gtacctgcgg taatagattt gagttcttca tcctctttac
    38701 tagtaccata aacaacttca cccggtttga tctccttaat catacttcca agcatcaaat
    38761 taatacctaa atccttaatt aagttgacac catattgcgc taattcatca tcaaacattg
    38821 gtaggatgcg agttgctgcc tctacacaaa taatttcaat ttgttcaggt tttacacccg
    38881 ctaaagcagc atattttggt cgctcatcaa ccatagctcc ggctaactca atacctgtaa
    38941 aaccagcacc acatactaag agtttgagaa gattttcatc ttttgtctca cggtacaact
    39001 tcattgtatt aacaatgtga ttatggatgt tttctgcttg cgaaatattg gtcatctgca
    39061 aagcattctc catagcaccc ttgatgccaa aagtttcaga tacaaagcct aaggcaacta
    39121 cgacataatc ataatggagt tcaccgttat ttttaactgt tactgtttta ttttcagcat
    39181 cgactctaag cacctcatct tgcatgaagt taaccttatt agtgttgata acatccttaa
    39241 ttgggaatgt aattttttct tttggctgag agcctgcagc tacttcatgt aattctgtag
    39301 cttcgtagtg ataatcattc atgtcaacaa gggtaatatg aaaatcacct gattgttttt
    39361 gtaaattacg aactgctttc aaaccggcat aaccagcacc taaaactagg atttctttca
    39421 tgataaaact cctctaaaaa atgattccaa ttagatacgt taagacttgg gttgcagatc
    39481 ccagtgctag aatacgtacc gcacaaacaa atgtttctct cttaacctgt ttttgccaca
    39541 atagttttgt ctgcttataa ataaatggta ttaataacaa actcaagagt actgtaacag
    39601 gcgcaagtcc aaaaagaaat tcaaaaacaa ttgccaataa agctatacta ttagaaatag
    39661 caaacagcaa gagtccacct cttatccctg tataatgtac aagggtatag cgatgatttt
    39721 tttcatcttc ttctttgtcg cacagattat tcgccaacat taggttggca atccataagg
    39781 tgttcggcaa ggaaatcaag aaaattttgc ttaagttgag gatatcccac gaaaagtttt
    39841 gataagtgtt tagatataca caaattaaac taatcataaa tcccattgtc aagccagaaa
    39901 atacttctcc taagggcaga ctagataaag gtcttggacc aaaggaatat aaaacaccaa
    39961 ttccaaaaca aaaaagtccc atccataaaa gaggcagacc gacttgggaa actaaaacta
    40021 aaccaatcat actagaagta atgactaaac ttgccataat aacttctatt tggcgtaggg
    40081 ataggttctc acgaccaata atgttagtgt cattcttata atccaaatcc acagcattcc
    40141 gataatcatt gtaattattc caaatgtcaa cgaacatatt gaataaaaac atagcaacga
    40201 agaataaacc gacatataca gggtgtactg aattgtagta ataagcacta aaacacaaac
    40261 caatcaaaaa tggtaaaaca ctagctgttt tggctttcat ttctaccaac tctagaaaga
    40321 ttggtaatgt cattattaaa gtcctctcta tacataatct tcaacaatac tattataatg
    40381 caatttctac catttttaaa acaatagggc ttgatttttg attaaaaata caaaaaattt
    40441 ttcttaaaat gagttaatca cacttagaac actaaatact attttgagga gtcctattca
    40501 tcacactatg ccatgtaata ttcggaaaaa tagtattgca accctcaact atcccttatg
    40561 ttggcaccag cctttgaaag tcctctgatt gcactgctta gttagtattt tcatcagctg
    40621 tatgatctct aagagccatc agcatactaa aaaaactagt attacacttt gaaaagtgta
    40681 acactagctc caatttgttt taaatagctt aactatttaa tcattaatct actttatata
    40741 tttaagcatt taaagttgaa tgttggttga ggatacctga gagagaacct tgaatataat
    40801 ttggcaatac ttgtttttga gcacgttcat agatttctga atctgttaac tgacgttctg
    40861 atttggggct tgatacttgc ggtgtcactg ctgcattttg ttgtttgtaa agtgttgaat
    40921 gctggttaag gatacctgag agagaacctt ggatatattt tggtaatact tgtttttggg
    40981 cactttcata aagttctgaa tctgttaact gacgttctga tttggggctt gatacttgcg
    41041 gtgtcactgc tgcattttgt tgtttgtaaa gtgttgaatg ctggttaagg atacctgaga
    41101 gagaaccttg gatatatctt ggcaatactt gtttttgggc acgttcataa agttctgaat
    41161 ctgttaactg acgttctgct tttggaggtg ttgcttgcgg tgctgtgaaa gttgaagctg
    41221 aaacaccttg tgtagaacca agagctacga atactcctcc agcaaacatt gctgagacaa
    41281 gaccaagttt caaatagtta gcagttgaga cgttgttgac ttttttttct gtaataagtt
    41341 tgttcataag aattacctct ttcttttggg ttatttattt tatgaactaa gtataacaac
    41401 aagaaataaa gtaaggttaa actaaatata aaataattct taagtcattc ttaggaaacc
    41461 ataaaatcat acactaacaa aaaatttggt atctagctca aaagtaaaag tagaataaag
    41521 gcttttactt ctgagctagt aatgaataaa ttatatttgc atctagtttg gaattagccc
    41581 tagtgtatta taatgatccc tacaaatatg tgccaaacac cttcttcata tatacaagat
    41641 tgatttaaca ttcctatacc cttaatggtg ggcacattct cttcatgttc catatagaaa
    41701 tcaacagata attccgtatt gtccctatta ttaaattttc cagttaatga taaattatat
    41761 catccacctt gaggtgtcct attaccaatc tgttcaacct ttacacttga cgaatcgaat
    41821 tgaacagatt ctgtcttatc attctgtgat ttagaacatc aacaatttct tgttcatgtt
    41881 ctttgaggta agctagttgt tcttctttaa cgtgctttta tctttcgtac ttactgatac
    41941 ttgagagcat ttttttgacc ctacttctta ctaatcagct gacgtcccta taagatgaat
    42001 cataaacaca tagctgccac taaccatttt aaccttttat gcatacataa ttcttcttga
    42061 tatcccatac cattatcttt agtataaaca cttaataaaa caagcattta acaactaagg
    42121 ttaataaaat tcaataaatc taatccttct acttacttta gtttatcact tttttctctc
    42181 atcctatgct ataatagtaa ggtatcaaaa tttaggagaa tatagtctta tgatgaatat
    42241 gcaaaatatg atgcgtcaag cacaaaagct tcaaaaacaa atggaacaaa cgcaagctga
    42301 tttagcagct agccagttta ctggaaaatc tgctcaagag ctcgttaccg tgacatttac
    42361 aggcgataaa aagctaattt caattgatta caaagaagct gtggtagacc ctgaagatat
    42421 tgaaacacta caagacatga caactcaagc tatcaatgac gctttaagcc aagttgacga
    42481 tgctacaaaa aaaattatgg gagcatttgc gggtaaaatg ccattctaaa aaataaatca
    42541 catttactga atcacctgta aatgtgattt ttatttcaaa cttctttcat tttttcacga
    42601 aaacaagttt tattttcaaa aaatatttgc aaaaaatcag cagagaggta taataacatt
    42661 gaaaatatta gagagaaggt gactaaccat ggttatggat agtctaatca taaaacagtt
    42721 gattaagagt acatttgata tacccttaca agtcacttat ccaaatggga atattgaaac
    42781 ttataatggt agcaatcccc acgtcaaact taaactaaac aaaaatttct ccgtctcgga
    42841 gctatcaaaa gacccttcca tagtattggg tgaagctgtt atggatggag acattgaaat
    42901 ttatggttct atacaagaat taattttatc agcttaccgc tgtggagaca gctttctacg
    42961 taatagtaaa tttagcaaat tgattcctaa acaatttcat gataaaaaac acagtaaatc
    43021 cgacattcaa aagcattatg acatcggaaa tgatttctat aaactgtggc ttgatgatac
    43081 catgacttat tcatgcgcct attttaaaca tgaaaatgat agtctagagc aagctcaact
    43141 aaacaaagta caccatattc ttaataaatt aaatgctcag ccgggaggga aattactaga
    43201 catcggttgt ggctggggga cacttatcat tactgctgct aaagaatatg gcttaaatgc
    43261 gacaggtatc accttaagtg aagagcaggc ttctttcatt actaagcgta tcaaggaaga
    43321 agggttggaa aataaagtaa cagtcctcat taaagattat cgtgacattc gtgaaacata
    43381 cgattatatc actagtgttg gtatgtttga acatgtagga aaagaaaatt tatctcaata
    43441 cttccaaacc atttctaaaa gactcaacat caatggtcta gctcttattc atggtattac
    43501 tggacaagtg ggtggtaatc atggtagtgg aactaattct tggattaata agtatatctt
    43561 cccaggagga tatatccctc gtttaactga aaatttaaat catatagcta gtgctggact
    43621 tcaaattgct gacttagaac cgcttcgtcg tcactatcaa aagactctcg aattatggac
    43681 aaaaaatttc cacaatgccc ttcctgaagt tcagaaaact catgataagc gttttatcaa
    43741 tatgtgggac ctctatcttc aatcttgtgc ggcctctttt gaatctggca atattgatat
    43801 ttttcaatac ttgctttcaa aaggtgtttc aaaagacact atgcctatga cacgcgatta
    43861 tatgtattct gctaattgat atagttaata ttatagtatt ccaacttatt ctaaataaaa
    43921 aggactctct tgaattcaag ggagttcttt aacgttcttc tctggctaac tctatacaat
    43981 gtgacttttg gtgacaatgt ggacagaata cttttcgcgt cttaggggta tgtttcgcta
    44041 gattaaatgc ttgatatgtc ggtgtaaagg tttgatgaca atttggacaa agataaacaa
    44101 cctgttgttt aaaataccaa accataccat tcatagctat taagaatact gttactgcta
    44161 tccacataag ccatctttga tttagagaaa ctgctgctat tattaacgta atatagacca
    44221 taataatcat aacgacttgt agcactatgc ttttttgtag tataccccat tttcttttgt
    44281 tttcttccat aagcacaatg tcagaaagat agcctaatgt tttactatct tgtttttcta
    44341 cagtttttag taggttgaca gctttgtcaa gtttgtcttt tttactatca atagctagcc
    44401 ttaattctct tatctacact tgtaaaaaaa gctctaaaat ctggctagca ttgtcttcag
    44461 taaatagatt tttgatatta tcaattgaaa aatctaaatc tcgtaaataa ataatttgtc
    44521 taagcttttc taaatcagca atactataaa ccctcctacc tccttcagat agttctgatg
    44581 gggataaaat tcctcttttg tcatagtact gaacagtacg aacagtaact cctgcttctt
    44641 tagctaagtc accagtggaa tatttttctg acatgtttca cacctccttt aatatagtaa
    44701 acacttcttt atcagaaaca cctttactat atcaaatgac ctaaggtaat aagcaagtaa
    44761 taacgctaag tatttttaac tttaaataaa actacaacaa ttaatcaaac aaatttccta
    44821 aagtcgcaaa tgggctatca actgctacct ctttttgctg ttttaaatag attttatttt
    44881 catccaagtc cagaaatttt tcataaacac gcgcagtcat acgggcatcc tctaagctat
    44941 tatgtgcttt accctttatt cctaaaaagt cagcaacagt agtcaattta aggttgacaa
    45001 taccatttaa atctgtactg cgtctcacaa aagcttcatc atataagtcc acctgatatt
    45061 gactagttag atccaaacca ttttcaacta aaaggggtaa atccgattta tacccattat
    45121 aaccaatcag tggtgtatct ccaacaaaac tctgaaagtc tgtcaaaact atctcaattt
    45181 taggagcccc tataatatct ctagctgtaa tacctgttaa accattaata aagctttgta
    45241 atggaacttt tgtgtgaaca taagtatcaa ataaagcaat ctcttgatga ttagaatatt
    45301 taacggccga cacttgaata atatggctat gctcaccaac cgtgttaaac tctaaatcaa
    45361 aagctatata ttcttgtaat tgtttcatta tttctcctaa aaagcgcaag gaaactcctt
    45421 acgcttttat tctatttttt attgaagaaa cgtccaaaga aacttttttt aacagcttct
    45481 aattcttgaa ttttttcttc tttggcttct tgaatctcct caacctctgc tttagcttca
    45541 tttaactcaa gttgcagacg ttgcgtatct tccatagcag ttaaagtcaa ttgctgttgt
    45601 tgatctaatt gcttatcttt ctctgctatt tgaatatctt ttacatgtaa ctgctcgtct
    45661 tttgaagcaa tctgaatatc ttttgctttt aactgttcat ataaacgtgt gatttcagta
    45721 ttcttctcat caacgagaat ttctaaaagt tctcgtcttg atgcttcttc gtcaattggt
    45781 tcctcttcca aaatagtttt tttatagatt tcttccaatt taatcaagcc atcacgatta
    45841 acaactgtaa cgcctttact attcttttcc acacattctt cagggagtgt tttaactctg
    45901 ttattcatcg cttgacgact aacccctaaa atttcagcta attcactaac cgtcttttca
    45961 actgtcataa cttcctcttt tgtactgatt ttcttataaa agaatatcac aatcctaatc
    46021 tagtgtcaat ttactgtcaa cttaaagtgt caaacaagac attttgacgt ctatttctct
    46081 atttatatgc tagaataaga ttatgaagca ttatgacaca ataattatag gtggtggacc
    46141 tgctggaatg atggcagcta tttcctcaaa tttttacggc aacaaaacac ttcttattga
    46201 gaaaaataaa cgtttaggca aaaaactagc tggaacaggt ggtggccgct gtaatgttac
    46261 caataatgga aaccttgatg agctcttagc tggtatacct ggaaatggcc gtttcctata
    46321 tagcgtcttc tcacaatttg ataaccatga tattattaac ttctttcaag acaacggcgt
    46381 cacacttaaa gaagaagatc atgggcgtat gttccctact actgataaat ctcgtactat
    46441 cattaacgcc cttgaaaata aaataaaaga acttggtggt caaattatga ctgacacaga
    46501 agttgtctct gtcaaaaaga taggagattc attctatatc aaaactaaag atacacagtt
    46561 cgcatccgat aaacttatcg taacaactgg tggtaaatcc tatccgtcta caggttctac
    46621 tggtttcggt catgacattg ctcgtcactt taaattagaa gtcacagata tggaagccgc
    46681 cgaaagccct cttttaactg attttcccca taaaaaacta caaggtatct ccttagatga
    46741 tgtcactttg tcatttgaaa agcatattat tacacacgat cttctcttta ctcattttgg
    46801 attatcagga ccagcagcct tgcgaatttc tagctttgtc aaaggcggag aaactatata
    46861 cctagatgtt ttgcccaata tctctgttaa agaattggaa atacacttcc aaaatgaaag
    46921 agaaaaatct ttgaaaaacg ctcttaaaat acttcttcct gagcgtttag ctgaatttta
    46981 cgctgaagac ttacccgaaa aagtaaaaca agtatctgta aaagacttag aaatgttaat
    47041 tcaaaaactt aaaaaacttc ctattttagt aactggcaag atgtctctag caaaatcctt
    47101 tgtaacaaaa gggggagttg atcttaaaga aataaacccc aaaacacttg aaagtaaaaa
    47161 ggtagctggt ttacattttg ctggtgaagt cctagacatc aatgctcata cgggagggtt
    47221 taacattact tctgccttat gtacaggctg ggtagctggt agcttacact attaatacat
    47281 gaaagctgaa caattccgtc agctttttta tatagaattt aaagataata tttggctctt
    47341 aaggcttctt tgattttttc caattcatct ggtaaattta cctgatgaat agcataccca
    47401 atctgataag gggatactat aaaacgagga acaattttac aaataagacc cccatcatgc
    47461 ttatagaaaa agagattata agatttacag cgccctccca agaattcatt taaaatataa
    47521 tgacacaaaa ttggtaaact atctgcaatc aaaatagcgt tttccaattt ggatacttta
    47581 attgtgaact ctgtgaaatc aagcatagga cgattggcaa tactaatctc tgcttgttga
    47641 ctcgtttccg aacaaacctc gataccatca aaccaatcta agttcaacgt ttcataagca
    47701 tctacgtttt ttaagccaac aatttggcta tgaggatgtc gtaaacttcc gccagataag
    47761 ggcccaaaat ttcgataaag aacgaccgaa cggtaatgac cactttcttc cattaattgc
    47821 cattttgaca aaagataatc taacaattgg cgcatctttg actcactata aactgaaata
    47881 tccccattgt gatcatttga ttcaatcacc aatgtttgat aggtttcttc taaagttggg
    47941 tatttatttt ctacccagat catatcgtct ttcacgtcta ggatttttcc taattggctt
    48001 ttatcacaaa atggacattt gtctttattg ctgggttttt gtttcgcgat attagggtta
    48061 aatcggatat atctttcatt catactgtta ttgtaacaca ttcatcacca ttacaatata
    48121 atacttatcc agttaacatt ttccttgcat tcttagctga aaagagtata atagaatcat
    48181 gtcttgttta ccggttaatt accagttaac taggcaccct gttttgggat agaaaaatct
    48241 ttttgggaaa ggagaaatca tggctaagaa atcaaaaatt gctaaatttc aaaaacagca
    48301 aaaactcgta gaacaatatg ctgaactacg tcgtgagtta aaagaaaagg gtgattacga
    48361 agcacttcgt aaacttccta aagattctaa ccctaaccga cttaaaaatc gtgatttaat
    48421 cgatggccgt cctcacgctt acatgcgtaa attcggtatg agtcgtatta acttccgtaa
    48481 cctagcttac aaaggccaaa ttcctggcat taaaaaagca agctggtaat tcgtgaagac
    48541 aaatagttac tttttatgac tattctattg agtgtggacg aatagttaat acattgtaat
    48601 tatttaacct gttgagtatt tactcagcta ttacaaactt cttcaaaatt ttattatcat
    48661 aggaatgtag gatctcatca atccttaaat gactaataat aaaagatgaa aaaatttttc
    48721 ttatctcaaa tcatagatgc caatttattt attttcaaaa agcccggtat gccaagatac
    48781 cgagcttttt attattataa atactgataa aattctttta gttaatccca aaaacaagac
    48841 tattattttg taatagtcac tactgaataa tatttctttt attaaaaact cacaagacat
    48901 ttcttaaagc agttacatct tcgcgactag ttgaccaact agttgctaga cgtattacag
    48961 tatgatggtc atcatatttt tcccaaaaac tataagctac attctttgct aaatctgcta
    49021 gttttgtatt ttctacaata ataaattgtt gattagttgg tgattttaaa tagaatgagt
    49081 aacctttttc ttctaaaatg atttttaatt cctctgctaa gtcgatcgca tgctttccaa
    49141 tttttaagta aaggttatcc gtaaagaaac gatcaaaagc aagccccagc aatcttccct
    49201 tggctaataa cgcaccatgc tgctttacaa ttgtagtaaa ctgttttgga cgatttttct
    49261 ttgtgaaaac aactgcttcc ccagcaagag cccccatttt ggttccacca atataaaaaa
    49321 catctgataa ggctgcaatc gttgggaaat caacatctgt atctttcgca gccaaaccat
    49381 accccagacg agcaccgtca ataaataagg ggatttgata atgtttacaa atcttcgata
    49441 actcttctag ttctgcttta ctataaagtg ttccatattc tgttggatgt gatatataaa
    49501 ccattcctgg aaaaaccatg tgttggtaat taccatcagc ataaaaagtc tcaatataag
    49561 ttgcgacttc gcttgcgagt aatttcccat tatgagaagg taaagtcaat accttatgcc
    49621 ctgaaaattc tatggctcct gcttcatgac tactcacatg acctgtttct gcagcaataa
    49681 ctccttcata agaagcaagc atactactga taactacttg atttgtttga gtaccaccca
    49741 ctaaaaactc aacttcagct tgtggacagg aacagacttc tcgaatttta tctgccgctt
    49801 gttgacagta actatctgtc ccatatcccg atagaccttg atcattagtt tcaataaggg
    49861 catttaaaag ctctggatga gctcctttat tataatcatt ttcaaaatgt aacattcctt
    49921 aactccttta ttagttaaaa aaatagcgac aatatcattt gctaatattt ttgaatattc
    49981 aatcattata acaaataaaa aatatcaaaa caagcgtttt gatattatga acacctataa
    50041 actctcaaaa gctaaactag gtttggcatt caattttaaa gaagcaaaat tctctttttc
    50101 aaactctatc gctgctgcta atgcaatcat tcctgcatta tccccacata atctaagagg
    50161 aggaataacc acatcaatat caggagaaat atcagtagcc aagcgttccc gaagtccttg
    50221 attagctgca actccaccag caacgactaa agttttcact ggatacttta acaaagcttt
    50281 ttgagttttg gccaataaaa tatccaaaac agccgcctga aaggaagcac ataaatcttc
    50341 aagaaccaat gcttcaccct tttgttctgc attatgatgt aaattgataa atgcagattt
    50401 taatccagaa aaagaaaatt caagatgatc ttctttgatc atcgctctag gaaaatggta
    50461 agtatcttga cccttatgag ctaactgatc aatctcgcga cctgctggat aagttaagcc
    50521 cataacacgg cctactttat cataagcttc tccaacagca tcatcccgag tttctcctac
    50581 tattttgtaa tctcccggtt cagaaacata tactaattct gtatgtcccc cactgacaag
    50641 caaagctaac aatgggtatt gaagttcctt aacgtcacgt gctgccatta aatgacctgc
    50701 catgtggttt ataggaatta gaggtaattt atttgcccaa gcgaaagctt ttgctgcagc
    50761 catacctacc aataaggctc ctactaatcc cggaccatat gttacagcaa cagcatccaa
    50821 atcgctagca acaatacctg cttcttgaag agcatcctca aaacaggttg ttactacttc
    50881 aacgtgatga cggcttgcca cttcaggaac aacaccacca aaacgtttgt gactttcaac
    50941 ttgacttgca ataatattag ctagtaactc tttatcattt tttaaaatag caacactagt
    51001 ttcatcacat gaactttcca ctgctaaaat atatctatct ttcataatga tattaatcca
    51061 tcaatatcgt cttttccatt agtattgagg actattcata tcctttctat catcaactat
    51121 ttatcccttg cgcatcacta tagcatcttc aattggatca tggtaataat ttttgcgctt
    51181 tcttaaaact acaaaaccac acctctcata aagtttttgt gctacgatat ttgaataacg
    51241 aacctcaaga aaaactggca cctctatatc ggcgatcatt gacattaact ggtaagcata
    51301 acctttacct tgataatttt ttttaacagc aatattagta atttctactt cacctaccaa
    51361 ttgttgaaga gccaaaaaac caatgacttc accgtcatta taaacaaaaa aataatctgt
    51421 tgaatctttt tttaaatctt ctgaaatctg cgataaactc cacggagatt tgtcatagac
    51481 atcagatagt acgctccata tagccagtgc ttgttctgat gattccatgt caaactctct
    51541 taatatattc ttctgtattc gtttcacagt ggttttttaa ccaattttcc tcagcttcaa
    51601 cacgttttaa gtatcgtgga acaaacgcat ctacattaac gcttttcatt ttttgtcctt
    51661 tgcgcccaat tgctactgca caaggtaaag tttctgtaat tttagcatgt ggtaaggctt
    51721 tcttaatctg atcaaaaaat gctgcaacct ctccgacaaa atgaacattg gctttattcc
    51781 ccacctcttg taagacttct tcaagagaag tgtgacagtc tggtttaaca gtatcaccat
    51841 tttgatagaa accaacataa acattgttac gtcgtgcatc tataagtggt accaataaat
    51901 cattttctga aaatccattt gttaaagcgt acaggctaga tactccaact aagtcaatct
    51961 taagcgtata agctagcatt tttgctgtag caacagctac acgtaagccc gtataagatc
    52021 ctggaccctc tgatactacg atacggtcca aatcttgagg ttctaaatca attgattgca
    52081 ttaaaaaatc aatggctggc atcaaattaa tgctatgatt ctttttgata ttgatagtga
    52141 cagtcgctaa acattccata ttgtttagta cagccactga tagtgctttg cttgaagtat
    52201 caaaggctaa aactttcatc atttaacttc ccttacttga ttctacctct tattttatga
    52261 tataatcatg ataattgcaa cgaatcaagg ttaattctat tactctataa aaacttaata
    52321 atggtttgtc attgtctcac ttaacggcat tgacccattt ttttatagtt agaaaaaccg
    52381 agagataaga aaggattatt atgatttata aagttttcta tcaagaaaca aaagaacgca
    52441 acccacgtcg tgagcaaacc aaaacacttt acgttactat tgatgcagct aatgaattag
    52501 aaggtcgcat tgctgcacgt aaactagttg aagaaaatac agcttacaat atcgaattca
    52561 tcgagttact ttcagacaaa caccttgaat atgaaaaaga aactggtgtt ttcgagctca
    52621 ctgagttcta gaaaggagtt aaacataata gctagagtaa tctacccatt atgataaact
    52681 tattatgtca aatatcaact taaaacccga agaagttggg gtttatgcga ttggtggtct
    52741 cggtgaaatc ggaaaaaata cttacggtat cgaatatcaa gacgaaatta ttattgtaga
    52801 cgctggtatt aaattccctg aagatgatct tttgggaata gattatgtta tcccagacta
    52861 ttcatatatc gttgaaaaca ttgaccgtat caaggccctt gttataactc atgggcacga
    52921 ggaccacatc ggtggtattc cttttctatt aaaacaagct aatttaccga tttatgcagg
    52981 tcctctcgct cttgctctaa tcaaaggtaa gttagaagaa catggtttac ttcgtgacgc
    53041 tacattatac gaaatccacg ctaatacaga gctgactttt aaaaacttaa gcgttacatt
    53101 tttccgcact actcactcca ttcctgaacc attaggtatt gttattcata cccctcaagg
    53161 taaggttatt tgtacaggtg acttcaaatt tgactttaca cctgttgggg agcccgctga
    53221 cttacatcgt atggctgctc taggtgaaga tggtgtcctc tgccttttat cagactcgac
    53281 aaatgctgaa gttccaacct tcactaattc cgaaaaaatt gttgggcaat ctattatgaa
    53341 gattatcgaa ggtatcgagg gacgtattat tttcgcctct tttgcttcaa acatcttccg
    53401 actccaacag gctgctgaag ctgctgttaa aactggtcgt aaaattgctg tttttggacg
    53461 gtcaatggaa aaggcaattg ttaacggtat tgaacttggc tatattaaag ttccaaaagg
    53521 taccttcatt gagccaagcg aacttaaaaa tcttcatgct agtgaagttt taattatgtg
    53581 tacaggtagt caaggtgagt ctatggcagc acttgctcgt atcgctaatg gtactcatcg
    53641 tcaagttact ttgcagcctg gtgatactgt gattttctca tcaagcccaa tccctggtaa
    53701 tacaacaagt gtcaataaat taatcaatac cattcaagaa gctggtgtcg atgttattca
    53761 tggtaaaatt aacaatatcc atacctccgg ccatggtggt caacaagaac aaaaattaat
    53821 gttacgcttg attaaaccaa agtatttcat gcccgtacac ggtgagtatc gcatgcaaaa
    53881 agtccatgct ggtttagcag ttgacacagg tattcctaaa gagaatatct tcattatgga
    53941 aaacggtgat gttttagctt taacgagcga ctctgctcgt attgctggac atttcaatgc
    54001 tcaagatatc tacgttgatg ggaatggtat tggcgatatt ggtgcggcag ttctacgtga
    54061 ccgccacgac ttatctgaag atggtgttgt ccttgccgtt gctaccgttg atttcgattc
    54121 aaaaatgatt ttagctggac cagacatcct tagccgaggc tttatctata tgagagaatc
    54181 tggggacctt attcgcgaaa gccaacatat cttattcaat gctattcgta ttgcactgaa
    54241 aaataaagat gcaagtatcc aatcagtaaa tggagctatt gttaatgcct tacgcccatt
    54301 cttatatgaa aaaactgagc gtgagcctat cattattcct atggtcctta cccctgataa
    54361 ataaacaaaa gaacggaaaa tactttctcc gttcttttta attgcctata ttagcaaaat
    54421 aagttaatat atcaaattac ttctgggata actatagaat ataaaagaac tccaaacagt
    54481 atcgtacttt tataccgtct atgtgaggag ttctttatag attaattcgg ataaatgtag
    54541 ctaactaccc ctggtgttac tgtattttgt ggatcaaacc aaccacgata attatcaatc
    54601 cattgatgac ctgcataatt agattctaaa acctgaattt tattagtgac aggatctaca
    54661 gctgtaacat aagccacatg tccataacta ccatcagtcc aacatacgat agaacccacg
    54721 cgcggttgtg tccctactgt ataaccagct gatgcggcgc tcgcagccca atcaccaccg
    54781 tttccccacc agttacctgc ccatgttgcc atctccttta caccccatgt gcattgacct
    54841 agtggaaacg tattactagc tggatgtgct tgagcaattt cctccgcact tgctaggtag
    54901 tctacatcta ctgcatagtt atatgtattc gcatctgcat ttgttgaagc aaatgaaaaa
    54961 gcggacacca aagccactgt tccaagcatt gttttagact ttaattgttt tatgttcatg
    55021 ttttcctcca tctgatgact cctagtttat caaatccata taacagacaa atgaaaacct
    55081 gtaacaagtt ttaggcaatt ttttacaata atttacaaaa aaactagttt tagactttac
    55141 aaaataaagc ctaaaactag ttataaacat tcttatgatt aatataaatc taggtaatta
    55201 tcaatttccc attgtgaaac ataagtcgca taacttgccc attcaatacg cttagcgtct
    55261 aagaaattag tatagatatg agttcctagg gccgctttta ccacttcatc ttcttcaagt
    55321 gctttaagag cattatgaag agtagatggt aaatcaacaa tacctgcttg acgacgttct
    55381 tctacagtca tagcatagat atttgtctca attggttctg gtgcttcaat tttattctcg
    55441 attccctcaa gtcctgatcc caaaagtact gataaagcaa gataaggatt tgctgttggg
    55501 tcaaccgagc gcaattctag gcgtgttccc atacctcgag atgcaggtac acgaataagt
    55561 ggcgaacgat tgcgacctgc ccaagcaatg taaactggtg cttcgtatcc tggtaccaaa
    55621 cgtttataag aattaacagt tggattaatg atagctgtgt aattgtaagc gtgtttcatt
    55681 aacccaccta aaaagtagta ggcatcctct gataattgca ttccacgtgg atcttctgga
    55741 tcaaagaaag cattgttccc ttcattatca aaaagagaca tattacaatg catacctgaa
    55801 ccattgatac caaacttagg ttttgccata aaagtagcat ataaaccatg cttacgagca
    55861 attgttttaa caaccaattt aaataattga atgttgtcac aggcttttaa aacatcatca
    55921 tatttaaagt caatttcatg ctgtccaact gctacttcat gatgactagc ttcaacttca
    55981 aagcccattt gtgttaagac attaacaatt tcacgacgtg tattatctgc taagtctgtt
    56041 ggagctaagt caaagtagcc tcctttgtca ttaacatcaa gtgttgggtt accattttca
    56101 tccattttaa ataggaaaaa ttcaggttct ggtcctaagt taaatgactt atagcccatc
    56161 tcttgcatac gtttcatatt acgcttaaga tttccacgtg gatctccggc aaaaggttct
    56221 ccttctgctg tatagatatc acagattaga cctgcaacag ctccattttc atctccccaa
    56281 ggaaagacaa tccaagtatc taaatcagga tataaataca tatcagattc attgatacga
    56341 acaaagccct caatagaaga tccatcaaac attgctttat ttgataaaac tttgtctaat
    56401 tgttcatctg tagccggaat ttcgacattt ttcattactc ctaagatatc tgtgaacatc
    56461 aaacggagga aggtaacatt tttttcctta acttcgcgac gaatatcttc tgctgtgata
    56521 gtcatatagt ctccttaata tctagcttga ctgcatgttt acaaatgcat atttccaatg
    56581 tgctgtgaag gacttgaaaa acgcccttga ttacggagct catcttgtaa aagtcgacga
    56641 acctcggcat ctgataatga cttttgtttc tctttggact tatgttgtct ttgcatgtat
    56701 tcatttttaa tatcagaaat atgcaaacca tcgctaatga agtcttttat ttccagtaag
    56761 cggtccatat cattcagtga aaacatgcgg cggttacctt cagtacgttc aggtgtgatt
    56821 aacccttgat cctcataata gcgaatttgt ctggcagtca aatctgtcaa cttcataaca
    56881 gctccaatcg ggaatacagc cattgtacgc ctaagttctc tttctttcat gatggcctcc
    56941 tttcattaaa ccattataaa ctttaaaaag tttgatgtca actctcatgt gagattttat
    57001 gacatcaaac tataatttgt tattttttag ttttttccga agtatttcaa tatctgtatt
    57061 aaccatcatt gcaataaaaa caccacaaaa agtttcaaaa atacgtgcaa aaacatatag
    57121 aattgtctct ccgcttggga tagataaagt tataataagt aatgcggctg ttcccccaat
    57181 aatacctgat ttattattac aagcaacatt aatcatgata cataacatgg tacaaatagg
    57241 aacaattaat aaagtgaccc aaaaagcttg atgaaatatc tcattgaatg caaaaaatac
    57301 taaggagagt aagccaccta tactattacc aataattcta gaaaaaccaa agtgcacgct
    57361 tttatcaaag tcttctctta aactaaatac agcagttaga gcaccaattt gaagcccgtt
    57421 ccaaccaaat aaatgaaaaa ccaataaaac taaaaagacc gataagcctg ttttaaatgt
    57481 tctcattcca aatttaaact ttttaatatc aaacttgtaa ttagataata atttttttaa
    57541 cgttttcata cctatccctt cactatatat tctaacatat caatccataa aacccttttt
    57601 gtaaacgttt tatttattac tagtatatct tttataaacc aatttatatc aaatcgtcat
    57661 aaacttttga aaattgcagt gtcaaccaaa taccactata tctaaaaact agtaaatgat
    57721 aaatgaagtc aaggaaagtt caataactct acattatcaa ctaaaaaaag acatgcatca
    57781 gcatgtcctt taatataaat tataatgact attatttttc agtcaatgct gccaaacctg
    57841 gtaatacttt accttcgagc aattccatgc ttgctccacc accagtagag atccatgaga
    57901 atttgtcagc acgaccaagg ttgatagcag ctgctgctga atcaccacca ccgatgattg
    57961 atttaacgcc tggttgttta acgatagcgt ccattacacc gattgtacca gcttggaagt
    58021 cagggttttc aaagacaccc ataggtccgt tccatacaac tgttttagca ccagtaagtg
    58081 cttcatcaaa tttagcgatt gatttaggac cgatgtcaag accaaggaac ccttctgaaa
    58141 ctgcttcacc ttcagtgtcg cgaacttcag tataaccagc aaatgcgttt gcttcttttg
    58201 agtcaactgg caagatcaat ttaccgtttg atttttcaag gaggtcttta gcaacatcca
    58261 atttgtcttc ttctacaagt gagttaccga tttcgatacc ttgagctttg tagaatgtgt
    58321 aagtcatacc accaccgata agaactttat cagctttttc aagaaggttt tcgataacac
    58381 caatcttatc agaaactttt gagccaccaa gaatagctac gaatgggcgt tctggagttt
    58441 caactgcttc ttggatgtaa gcaatttcgt tttcaagaag gaaaccagct acagcttttt
    58501 caacgtttgc tgaaatacct acgtttgatg catgagcacg gtgtgctgta ccaaatgcat
    58561 cgttaacgaa gattccatct ccaagtgaag cccagtattt accaagttct tcgtcattct
    58621 tagattcttt cttaccgtca acatcttcaa aacgagtgtt ttcaaccaaa agaacttgtc
    58681 catcttccaa agcattgatt gcttcttcta attttgcacc acgagtaaca cctgggaata
    58741 caacatcttg accaagttta gcagctaaat cagcagctac cggtgcaagt gattttcctt
    58801 ctttgtcagc ttcttcttta acacgtccaa ggtgagagaa gaggatagca cgaccacctt
    58861 gttcgatgat atacttgatt gttggaagag ccgcagtgat acggttgtcg ttagtgataa
    58921 cgccgtcttt caaaggcaca ttaaagtcaa cacgaacgag gactttttta cctttcaaat
    58981 caacgtcttt aacagtcaat ttagccatta gtaaaactcc ttatataatt tgatattatt
    59041 tattataaca tatttttgtg aacagttgta attattgatt actttttcac aaatagattt
    59101 catagatttt cctagttgtt tttaagagaa ttcgtatact tattaatcat acgaatgcaa
    59161 cattttttgt cgttctttca attgtttttg aacatccaga tgttttcctt gataaatagc
    59221 actttcccaa gaaccgtaca taggatttgg gaaaacaata aatttactac caaactcact
    59281 ttgaagttta gttagtagtt gttctctatc tgtactagat gatttagaaa aatcagcaaa
    59341 atcaactaaa ttatctccaa aaagcataat taaattggta tctttttgaa ctgcctgacg
    59401 gcgactctct ttagatttca ttcctttttt aaggaaaagc aagtggtctt tcccttgaac
    59461 aggtatacct tccttctcta aattttcttt agtcgcatca acttgagcat ctgtacgatc
    59521 tgagacataa taaattttta ttcccttttc attagcatac ttcaaaaatt ctttggcacc
    59581 cgcaacagcc ttagctgatt tcttttgcac ccatttatcc caactctctg gcgtgaaact
    59641 agagccatct ttaatattct ttgcttgata tgggctatta tctaaaactg tttcatctaa
    59701 atctaagata attgaatatg gtttttcact gggcttttgt aaccaatcat ctaacttcat
    59761 tttagcaaca ttataacctt gtaaatataa agccttggct tctgcagaat tttgatacca
    59821 aaggacagac atagtgtttt ccttagccaa cagtttatta gaacttagag aaatattttt
    59881 aaatttcgtc ttttgctcca ccttatctgg agacatatca gcttttttag tactgtggtt
    59941 tgactgacat g
//
LOCUS       DATRSG010000004        58683 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRSG010000004 DATRSG010000000
VERSION     DATRSG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58683
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.004"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..1226
                     /gene="fbsB"
                     /locus_tag="VBD89_000191"
     CDS             <1..1226
                     /gene="fbsB"
                     /locus_tag="VBD89_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-binding surface protein FbsB"
                     /protein_id="HEO7134749.1"
                     /translation="ISIDDDDYEKGNKELLREKLNIILNLSKRDYRVTPYYEVNKLHT
                     GLILLEDVPNLKIAKDKLFSLENSLKEYKGEKVNYEELRFNTEPLTSYLENKEKFLVP
                     NIPYKNKLILREEDKYSFEDDEEEFGNELLSYNKLKNEVLPVNITTSTILKPFEQKKI
                     VEDFNPYSNLDNLEIKKIRLNGSQKQKVEQEKTKSPTPQKEAVKEQTEQKISGKTQNV
                     EKKSETVATPQQSSTAQTSVQQPAPVQPVVQESKASQEEINAAHDAISAYKSTVNIAN
                     TAGVTTAEMTSLINTQTSNLSDVEKALGNNKVNNGAVNVLREDTSRLENMIWNRAYQA
                     IEEFNVARNTYNNQMKSETVPVDNDIEAILAGSQAKISHLDNRIGARHMDQAFVASLL
                     EVTEMSKSISSRIKE"
     gene            1293..1838
                     /locus_tag="VBD89_000192"
     CDS             1293..1838
                     /locus_tag="VBD89_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134750.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      1989..2078
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2234..2800
                     /gene="thiW"
                     /locus_tag="VBD89_000193"
     CDS             2234..2800
                     /gene="thiW"
                     /locus_tag="VBD89_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO7134751.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            2797..3351
                     /locus_tag="VBD89_000194"
     CDS             2797..3351
                     /locus_tag="VBD89_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7134752.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            3355..4641
                     /locus_tag="VBD89_000195"
     CDS             3355..4641
                     /locus_tag="VBD89_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7134753.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKWLIRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4629..5297
                     /locus_tag="VBD89_000196"
     CDS             4629..5297
                     /locus_tag="VBD89_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO7134754.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MIFK"
     gene            5381..6061
                     /gene="tenA"
                     /locus_tag="VBD89_000197"
     CDS             5381..6061
                     /gene="tenA"
                     /locus_tag="VBD89_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO7134755.1"
                     /translation="MLKDEMIAKGTKYIQSIYQDPFIQGIVKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKAVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            6063..6860
                     /gene="thiD"
                     /locus_tag="VBD89_000198"
     CDS             6063..6860
                     /gene="thiD"
                     /locus_tag="VBD89_000198"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7134756.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            6862..7632
                     /gene="thiM"
                     /locus_tag="VBD89_000199"
     CDS             6862..7632
                     /gene="thiM"
                     /locus_tag="VBD89_000199"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO7134757.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7646..8317
                     /locus_tag="VBD89_000200"
     CDS             7646..8317
                     /locus_tag="VBD89_000200"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO7134758.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            8444..9703
                     /locus_tag="VBD89_000201"
     CDS             8444..9703
                     /locus_tag="VBD89_000201"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7134759.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9805..10359
                     /locus_tag="VBD89_000202"
     CDS             9805..10359
                     /locus_tag="VBD89_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7134760.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            10352..11635
                     /locus_tag="VBD89_000203"
     CDS             10352..11635
                     /locus_tag="VBD89_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7134761.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11658..12518
                     /locus_tag="VBD89_000204"
     CDS             11658..12518
                     /locus_tag="VBD89_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO7134762.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLAKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12520..13440
                     /locus_tag="VBD89_000205"
     CDS             12520..13440
                     /locus_tag="VBD89_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO7134763.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(13457..13912)
                     /locus_tag="VBD89_000206"
     CDS             complement(13457..13912)
                     /locus_tag="VBD89_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO7134764.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQLQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      13983..14096
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14094..14603
                     /locus_tag="VBD89_000207"
     CDS             14094..14603
                     /locus_tag="VBD89_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO7134765.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELTSLLVGAVLVDK
                     LSRHVDFTK"
     gene            14743..16701
                     /gene="ligA"
                     /locus_tag="VBD89_000208"
     CDS             14743..16701
                     /gene="ligA"
                     /locus_tag="VBD89_000208"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO7134766.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            16713..17732
                     /locus_tag="VBD89_000209"
     CDS             16713..17732
                     /locus_tag="VBD89_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7134767.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            17736..20036
                     /gene="pulA"
                     /locus_tag="VBD89_000210"
     CDS             17736..20036
                     /gene="pulA"
                     /locus_tag="VBD89_000210"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO7134768.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQDPFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
     gene            20241..22109
                     /gene="glgB"
                     /locus_tag="VBD89_000211"
     CDS             20241..22109
                     /gene="glgB"
                     /locus_tag="VBD89_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO7134769.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLDVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKIQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            22151..23290
                     /locus_tag="VBD89_000212"
     CDS             22151..23290
                     /locus_tag="VBD89_000212"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO7134770.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            23280..24413
                     /gene="glgD"
                     /locus_tag="VBD89_000213"
                     /pseudo
     CDS             23280..24413
                     /gene="glgD"
                     /locus_tag="VBD89_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            24410..25840
                     /gene="glgA"
                     /locus_tag="VBD89_000214"
     CDS             24410..25840
                     /gene="glgA"
                     /locus_tag="VBD89_000214"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO7134771.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLGLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            26172..26372
                     /locus_tag="VBD89_000215"
     CDS             26172..26372
                     /locus_tag="VBD89_000215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO7134772.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            26404..27120
                     /gene="atpB"
                     /locus_tag="VBD89_000216"
     CDS             26404..27120
                     /gene="atpB"
                     /locus_tag="VBD89_000216"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO7134773.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            27138..27635
                     /gene="atpF"
                     /locus_tag="VBD89_000217"
     CDS             27138..27635
                     /gene="atpF"
                     /locus_tag="VBD89_000217"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO7134774.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            27635..28171
                     /locus_tag="VBD89_000218"
     CDS             27635..28171
                     /locus_tag="VBD89_000218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO7134775.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            28187..29692
                     /locus_tag="VBD89_000219"
     CDS             28187..29692
                     /locus_tag="VBD89_000219"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO7134776.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            29708..30589
                     /locus_tag="VBD89_000220"
     CDS             29708..30589
                     /locus_tag="VBD89_000220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO7134777.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            30663..32069
                     /gene="atpD"
                     /locus_tag="VBD89_000221"
     CDS             30663..32069
                     /gene="atpD"
                     /locus_tag="VBD89_000221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO7134778.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            32082..32495
                     /locus_tag="VBD89_000222"
     CDS             32082..32495
                     /locus_tag="VBD89_000222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO7134779.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            32560..32790
                     /locus_tag="VBD89_000223"
     CDS             32560..32790
                     /locus_tag="VBD89_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO7134780.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            32853..34124
                     /gene="murA"
                     /locus_tag="VBD89_000224"
     CDS             32853..34124
                     /gene="murA"
                     /locus_tag="VBD89_000224"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7134781.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            34126..34317
                     /locus_tag="VBD89_000225"
     CDS             34126..34317
                     /locus_tag="VBD89_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7134782.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            34392..35249
                     /locus_tag="VBD89_000226"
     CDS             34392..35249
                     /locus_tag="VBD89_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7134783.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            35540..36580
                     /gene="pheS"
                     /locus_tag="VBD89_000227"
     CDS             35540..36580
                     /gene="pheS"
                     /locus_tag="VBD89_000227"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO7134784.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            36663..37183
                     /locus_tag="VBD89_000228"
                     /pseudo
     CDS             36663..37183
                     /locus_tag="VBD89_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            37237..39642
                     /locus_tag="VBD89_000229"
     CDS             37237..39642
                     /locus_tag="VBD89_000229"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO7134785.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(39711..40613)
                     /locus_tag="VBD89_000230"
     CDS             complement(39711..40613)
                     /locus_tag="VBD89_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO7134786.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            40791..44024
                     /gene="rexB"
                     /locus_tag="VBD89_000231"
     CDS             40791..44024
                     /gene="rexB"
                     /locus_tag="VBD89_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO7134787.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            44014..47637
                     /gene="addA"
                     /locus_tag="VBD89_000232"
     CDS             44014..47637
                     /gene="addA"
                     /locus_tag="VBD89_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO7134788.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNYLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQ
                     SEDIVHAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSIKAA
                     IQIEKINYFFQENSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIERIDKYLILLGGKQLEVV
                     KMD"
     gene            47650..48567
                     /locus_tag="VBD89_000233"
     CDS             47650..48567
                     /locus_tag="VBD89_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7134789.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(48599..49975)
                     /gene="mnmE"
                     /locus_tag="VBD89_000234"
     CDS             complement(48599..49975)
                     /gene="mnmE"
                     /locus_tag="VBD89_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO7134790.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARHG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            50103..52013
                     /locus_tag="VBD89_000235"
     CDS             50103..52013
                     /locus_tag="VBD89_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7134791.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            52163..53131
                     /locus_tag="VBD89_000236"
     CDS             52163..53131
                     /locus_tag="VBD89_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO7134792.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            53206..54204
                     /locus_tag="VBD89_000237"
     CDS             53206..54204
                     /locus_tag="VBD89_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO7134793.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            54331..55719
                     /locus_tag="VBD89_000238"
     CDS             54331..55719
                     /locus_tag="VBD89_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO7134794.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            55779..57536
                     /gene="lpdA"
                     /locus_tag="VBD89_000239"
     CDS             55779..57536
                     /gene="lpdA"
                     /locus_tag="VBD89_000239"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO7134795.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            57634..58623
                     /locus_tag="VBD89_000240"
     CDS             57634..58623
                     /locus_tag="VBD89_000240"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7134796.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT        19192 a         9123 c        11898 g        18470 t
ORIGIN      
        1 atatctctat agatgatgat gattacgaaa aaggaaataa agaattgcta agggaaaaat
       61 taaatattat tctaaatctt tcaaagagag attacagagt aactccatac tatgaagtga
      121 ataaactaca tacagggctt attttattgg aggatgtccc taatttaaag attgctaagg
      181 ataagttgtt ttcattagag aattctttaa aggaatacaa aggagagaaa gttaattatg
      241 aggaactaag attcaatacg gaacctttaa ctagttactt agaaaataaa gaaaaatttt
      301 tagtccccaa tattccatat aaaaataaat taattttaag ggaagaagat aaatatagtt
      361 ttgaagatga tgaagaagag tttggaaatg aacttctaag ttacaataag cttaagaatg
      421 aagttttacc tgttaatatt acaacttcta ctatattaaa accgtttgaa cagaagaaaa
      481 ttgtggaaga ttttaatcct tattctaatt tagacaattt agaaataaaa aaaataaggt
      541 tgaatggctc ccaaaaacaa aaagtagaac aggaaaaaac taaatcgcca actcctcaaa
      601 aagaggctgt aaaagaacaa actgagcaaa aaatatctgg gaagactcaa aacgtagaaa
      661 agaaatctga aactgtggca actccacaac aaagttcaac tgcgcaaact tctgtccaac
      721 agccagctcc ggttcaacca gttgttcaag aatctaaagc ttctcaagag gagattaatg
      781 cagcacatga tgccatttca gcgtataaat caacagtcaa tattgctaat acagccggtg
      841 taacaactgc ggaaatgacc tcgctcatta atactcaaac ttctaatctt tctgatgttg
      901 agaaagcttt agggaataat aaggttaata atggtgcagt caatgtattg agagaagata
      961 catctcgtct tgagaatatg atttggaatc gtgcttacca agctattgaa gaattcaacg
     1021 tcgctcgtaa tacttataat aaccaaatga agtcagaaac agttccagtt gataatgata
     1081 ttgaagctat tttagcaggt tctcaagcta aaattagcca tttggacaat cgtatcggag
     1141 cgcgccacat ggatcaagct tttgtagcta gtttattaga agttactgag atgagtaaat
     1201 caatctcatc gcgtataaaa gaatagacac tgctatcaag gcgatcttaa acttttgtat
     1261 tgagctaacc taaaagatag aaagagacta atatgaaaaa aataacaact ttaatcttag
     1321 ctagtagctt attactagtt gcaacgacat cggttaaagc tgatgataac tttgaagtac
     1381 caacgcgtta tgttaaaatg agtgaaaaat caaaagcatt ttatcaaaga ttacaagaaa
     1441 aacaacgtaa aacacatact actgtgaaga cttttaataa ttcagaaata aggcatcaac
     1501 tacctcttaa acaagaaaag gctaggaatg atatctacaa tttaggcatt cttatttctc
     1561 aggagtctaa agggttcatc caacgtattg ataatgccta ttctttggaa aatgtctcag
     1621 atattgttaa tgaagctcag gctttgtata aacgcaacta tgatttattt gaaaaaatca
     1681 aatctacacg tgataaggtt caagtcttac ttgcatcgca tcaaggtaat acagacttaa
     1741 aaaactttta tgctgagttg gacgatatgt atgaacatgt ttatctcaac gaaagtagag
     1801 tggaggcgat aaacagaaat atccaaaaat ataattagtt tctaaactaa caaacattcc
     1861 taaatataag acattaaacc ctacttattg attagtgagt agggttttac tgttttaaat
     1921 agctttctgc tcagaatgta agccttgtca tttcaaagga actatgttat tattcttaag
     1981 taaattaaat aggacatttg gggtgcgtaa cagctgagat tatacccatt gaacttgata
     2041 cagttagtac tgtcgaaggg aaatgtgtgc cattacaagt tttttgtaat ggaaaagggc
     2101 gtagtgtctt cttaggagac agctagctct agttaatcag ataataaact ccgtttgctt
     2161 gcagatggag ttttttgtcg tcaaaatttt ttataggttg ttgcccctta tctgtcatag
     2221 agaaggagtt atgatgacta atcataaaac aaaaaaatta acggttttag caattatgat
     2281 tgctttagat gtttttctaa cgcccatttt tagaatagaa ggcatggcct ttatgtctag
     2341 tgtggttaat attatcgctg gagttataat gggaccaatc tatgcattag ttatggcact
     2401 tgtaacggct tttattcgta ttttaactca agcaggagta gcatctgttg cacctttagc
     2461 tattctagga gctggtccag gagctttctt agcgggttgg ttttatcggt tttatgcgaa
     2521 agactggatg tcttgggtgg gtgagtttat aggaacaggg ataattggag cacttttatc
     2581 agctccaatt atggtctggt tttggacttg gacagcggat ggaaatcacg aagtgttagc
     2641 taaagcatct gcagctcaat ccatttttct ttttatgcca aggtttatag gggcgactat
     2701 aattggcggt gttattgctt tgttgctact tcagggatta aagcgggttc ctgtatttat
     2761 gacaatacaa gcattattta aaggagaaaa agtaaaatga aattgggttt aaaagatgta
     2821 ttattgattg ctttattagc ggttgttctg ggcgttgttt actttggagc aggctatatt
     2881 tcaaatgctt ttgtaccttt tgtaggtcct atagcgcatg aagttattta tggtatttgg
     2941 tttgtggcag gtccaatggc tctttatata ttgaggaaac caggaaccgc tattgtagca
     3001 gaattgttag ctgctttgat agaggtttta attggatcaa tttatggacc aagtgtactc
     3061 gttattggta cccttcaagg tttagggtca gaattaggct ttacgctgtt taggtatcat
     3121 aattacaagt tgccagcttt tatattgtct gctattttga catcaatttt tagttttgca
     3181 tggagttttt atgccaatgg acttagtgca ttttcatttt catataacat tctgatgctt
     3241 atagtaagaa ctgtttcatc tattattttc ttcttattaa caaagaatat atgtgaccaa
     3301 ttgcatagaa gtggtgtact gaatgcttat gggattcatg aagggaagta gcctatgcta
     3361 tctgttgaaa aattagctta cacccatggc gatagtcatt atttatttga tgaagtgact
     3421 tttagtctta acccaggaga acgaatttta attagtggct attcagggtg tggtaagagt
     3481 acattggctc ttttactatc agggttaaaa gaaagtggga aaggtcaagt tttattaaac
     3541 ggctcattaa tagaaccaag cgatgtgggt tttctttttc aaaatcctga tttgcaattt
     3601 tgtatggata ctgtggcgca tgagttgtat tttattttag aaaatttaca aatagaaccg
     3661 gagcaaatgc aagatagaag tgagtttgta ttggcacaag ttggtttaaa aggatttcag
     3721 aatcgtttga tttatacttt atctcaaggt gaaaaacagc gattagcatt ggcgactatt
     3781 tttttaaaat ctcctaaact tattatttta gatgaagctt ttgcgaattt ggatcaagag
     3841 agtgccagtc aactattaca attagttctt aattatcaag caaacaatca atcaatgctg
     3901 attgtaatcg atcatttgat tacttattat caagatataa tggatcacta tttttggttg
     3961 gaaaaatggt tgataagggt cgattttgat tatatgctca atcgtttgaa tgtcttcgaa
     4021 ttagaaaaga aaagtcataa tacaggtgac aaactgttat caattaaaga tttccaagtt
     4081 aaactttcaa aaaacaaatt catctcttac cttgattttg atttagcaag tggggagagg
     4141 ttgtgcttag atggaccaag tggcgtgggg aagtctagtt tctttatggg attgttaggg
     4201 ctctatagga ctaaaggaaa gaaacaattt actcatagaa agcaaattcc tataagcttt
     4261 ttatttcaaa atcccttaga tcaatttatt ttttcaacag tttatgacga aatttttcag
     4321 gtttgcaagg atagtaacaa agcgagggat attttggaaa ccataaatct ttgggataaa
     4381 aaacagtttt caccatttca actaagtcaa ggccaacaga ggcgattagc cattggcagt
     4441 attttagcta gtgatagtaa acttctatta ttagatgaac caacttatgg acaagatgcc
     4501 tatcacgcta atatgattac gactcttctt ttgagttatt gtcacaaaaa tcattgtggg
     4561 gttattttta ctagccatga tcctcactta aaagagtgtt ttgcaacacg tttcttggag
     4621 gtgtcatctt gttcaatttg aaaaactatc atccagctat caccttccta tgtttattaa
     4681 taccaacctt ttcgtttagt ttcacattga ggctaagaac gtctttgtta tttctaatta
     4741 tagtagtaac actgcaatgc ttcgtaaagg tttctttaaa aacttgggcg aaggtcaatt
     4801 tgatctcatt tgtgatggga ctctctttat ttttagggac gtatttttgg gggaaattac
     4861 cccatcaatt cgtcttagct agtctagtgg cctgtcgtcc gcttattttt atgaacgtgg
     4921 gtttgttatt tcatgcatca cattctaatt atgattttat agaatctctt tatcaaactt
     4981 tcaaagtgcc tagtcatttt gcatatggta tttttgcggt ctttaatctt ttaccattaa
     5041 ttaaattaca ataccaacgt aatcgactcg cattccgact gaagaatcag gtaacttggg
     5101 cattatcgcc tagactaata ttgtcagttt tattaaagac gatatattgg gtagaacagt
     5161 tagagttagc aatgctttct aaaggttttg aagtgggtaa agagcgaaca catgcttcaa
     5221 cttatcccgt tcgttttcga gattatagtt tacttggtat gagtatttta ttggctatag
     5281 gaatgatttt taaataagta caattagttt agtaatacaa ttgtgcattt atatagagtg
     5341 agcatgttct gctcatgaga tcataacaaa ggagattact atgttaaaag atgagatgat
     5401 tgctaaagga acaaaatata tacaatccat ctatcaagat ccatttattc aaggaattgt
     5461 aaaagggcgt ttagaccatg acgtaatctg ccattattta caggcagata atatctacct
     5521 tgggaaattt gctgatattt atgccctgtg cttggcaaaa tctgataatt tacgagataa
     5581 acaatttttc cttgaacaag ttgactttac attaaatcgg gagttagcag atggtgaagg
     5641 tccacatcaa gccttggcag cttatactaa tcgatcttat caggatatta tcgaaaaggc
     5701 tgtatggtac ccgtcagcag atcattacat taaacatatg tattttcatt tttacgaaaa
     5761 tggtattgct ggtgctttag ctgcaatgag cccatgtcca tggatttacc atcaattggc
     5821 taaaaaaata attgaggaga accagttttt gaatggtaac ccttttaata attggataac
     5881 tttttatgct aacgatacag ttgaagagtt aatggaaaac tattttcgta tgatggatta
     5941 ttatgcgcag aatttgtcaa aagagaagca agctgatttg gtagatgctt ttgtgaaatc
     6001 ttgtcagcat gagagacgtt tttttcaaat ggcaataaat caagaaaaat gggaggatta
     6061 atatgactta tttagcacca gttttaacta ttgcaggaac tgatcctagc ggtggagcag
     6121 ggattatggc agatttaaag acatttcaag ctcgtcggac ttacgggatg gctgttgtga
     6181 ctagtgttgt tgctcagaac acttgtgggg ttagaggcgt tcagcatata gaaacagcta
     6241 tcattgatca acaactagct tgtgtttatg atgatattaa gccaaaggcg gtcaaaacag
     6301 gaatgttagc ggagagggaa acgattagtt tagtcgcttc atatttaaaa aaatatccgc
     6361 aaccttatgt attagatcct gttatggtgg caactagcgg acatcgttta attgattcag
     6421 atgccgtaga agcattaaaa gaagacttat tgccattagc aactattatc acaccaaatt
     6481 taccagaagc tgaggtttta gtgggatatg acttatcaga tgaagtaagt attattaaag
     6541 caggttacga tattcaaaaa caatattctg ttagaaatgt tcttataaag ggaggacatc
     6601 tagatggttt ggctaaagat tatttatttt tggaaaaaga agggttgata actcttagta
     6661 atcaacgtat caatactatt catactcatg ggactgggtg tacatttgca gcagtcgttg
     6721 cagcggagtt agctaaaggg caatcaattc taaacgcggt aagtaccgct aaatcattta
     6781 ttacttcagc tatcgaaaca gcgcctgagt tgggtcttgg aaacggtcct gtcaatcata
     6841 ctagctatca aggagactaa aatgtattta gaacaactaa aagaggtaaa tcctttaacc
     6901 atttgtatca ctaataatgt ggtcaaaaac tttacagcca atggtttatt agctttagga
     6961 gcttctccgg ctatgagcga gtgcatagag gacttagaag acttgttaaa agttgcagat
     7021 gcattattaa ttaatatagg aacactaaca aaagaaagtt ggcaacttta ccaagaagca
     7081 atcaaaatag caaataaaaa ccaagttcca gttgtattag atcctgtagc ggcaggagca
     7141 agtcgttttc gattagaagt gtcacttgat ttattgaaga attatcctat tagtttactt
     7201 agaggaaatg gtagtgaaat cgctgctttg gttggtgaaa aacaagcgtc taaaggggca
     7261 gatgggggaa aagtggctga tttagaatca attgctgtaa aagccaatca agtttttgat
     7321 gtgccagtgg ttgtcacagg agaaactgat gctatcgctg ttagaggaga agttcgctta
     7381 ttacaaaatg gtagtccttt aatgccactt gttacaggaa ctggatgtct attaggggca
     7441 gtattggctg cttttattgg tagttctgac agaagcgatg atcttgcttg tctgacagag
     7501 gccatgactg tttataatgt tgctggagaa attgcagaga aagtggcaaa aggtaaggga
     7561 gtgggaagtt tccaagttgc atttttagat gccctttctc agatgaaatc agaaatgatt
     7621 atggacaaat aggcggtgtc agtagatgaa agatacattg aaattatatt ttgtttgtgg
     7681 gactgtggat tgctctagga agaatatatt gactgttgtt gaagaagcct tacaagcagg
     7741 tataactcta tttcaattta gagaaaaagg ttttacagca ttacaaggta aggaaaaaat
     7801 tgctatggct aagcaactcc aaatactttg taagcaatat caagttcctt ttattattga
     7861 tgatgacatt gacttagttg agttaattga tgctgatggt cttcatattg gacaaaatga
     7921 tttacctgtt gatgaggcac ggagacgttt accagataaa atcatcggtt tatctgtgag
     7981 tacaatggct gaatatcaga aatcacaatt atctgttgtt gattacatcg gaattggtcc
     8041 ttttaatccg acacaatcaa aagcagatgc taagcctgct gttggcaata ggacaacaaa
     8101 ggcagttaga gaaattaatc aagatattcc aatcgttgca ataggcggaa tcactagtga
     8161 ttttgttcat gatattatag aatcaggcgc agatgggatt gctgttatat cggctatttc
     8221 aaaagcaaat catattgtag atgcaaccag acaattgcgc tatgaagttg aaaaagctct
     8281 ggttaatagg caaaaacaca gtgatgtaat caaataaggc tacatcgtta attataagaa
     8341 tttttaagat attatatcat cacacaatac tggtatttga aaaagaattt aaactatgat
     8401 aatatagtag agttgaattc tagttaaaga aagatttgaa agtatgcgta aaattattat
     8461 taacggtggc aagcaactga cgggagaagt tgctgtatcg ggtgccaaaa atagtgtagt
     8521 tgccttgatt cctgccacta ttcttgctga tgatgttgtg gtcttagatg gagttccggc
     8581 tatttctgat gttgacagtt tagttgacat tatggaaact atgggagcta agattaagcg
     8641 ttatggtgag acgcttgaaa ttgatccacg tggcgttaaa gatattccaa tgccttatgg
     8701 caaaatcaat agtcttcgtg catcctatta tttctatgga agcttattgg ggcgttatgg
     8761 tcaagcaaca ttaggtttac ctggtggttg tgatttaggc ccccgtccga ttgatttaca
     8821 tttaaaagct tttgaagcga tgggagctag tgtttcttat gaaggtgatt caatgcgtct
     8881 agcaacaaac ggaaagcctt tgcaaggagc aaatatttat atggatactg tcagtgttgg
     8941 tgcgacaatt aacactatga tcgcagcagc taaggcaaat ggtcgtacgg ttattgaaaa
     9001 tgctgcgcgt gaaccagaaa ttattgacgt ggctactctt ttaaataata tgggagctca
     9061 tattcgtggt gctgggactg atgttattac aattgaaggt gtgaaatcgc tccatggcac
     9121 gcgtcatcaa gtaattccag atcgtattga agcaggaact tatattgcta tggcagcagc
     9181 tatcggacga ggaataaaag tgaccaatgt gttatatgaa caccttgaga gtttcattgc
     9241 taagttagat gagatgggag ttcgaatgac agttgaagag gattcaattt ttgtcgaaga
     9301 acaagaaaag ttaaaagctg tatccataaa aacctcacct tacccaggat ttgctacaga
     9361 tttacaacaa cctctaaccc ctttattact aacagcagaa ggaaacggga gtttattaga
     9421 tactatctat gagaaacgtg ttaatcatgt tcccgaatta gcacgtatgg gagctaatat
     9481 ttctacccta gggggcaaaa tagtttatag tggtcctaat caattatctg gtgcacctgt
     9541 aaaagcgaca gatttgcgtg caggcgctgc tttagttatc gctggcttaa tggcagaggg
     9601 acgaactgaa attacgaata tcgagtttat tttacgtgga tattctaata tcatcgagaa
     9661 actaacatca ttgggtgcgg atatccaatt ggttgaggaa tagtatatat aacaaagagt
     9721 tgagaatatt tcttagctct tttcttcttg ttttagatta agtcacttat aggtatttga
     9781 aatctcttgt ggtataatat tatcatggat atatggacaa atttaggacg gtttgcattc
     9841 attgaaacag aacatgttaa cctacgtcct gttgcctaca ctgataggga agctttttgg
     9901 cgaatagctt caaaaaggac aaatttacaa tttatttttc ctgttcaaac cagtaagaag
     9961 gaaagtgatt ttcttttagt tcattctttt atgaaagagc cgttgggagt ttgggcgatt
    10021 gaagataaag ttagtcataa aatgtttgga gttatccgtt ttgaaaacat tgatttatct
    10081 aaaaagacag ctgaaattgg ctattttttg aaagaaagtt cttggggtca aggcattatg
    10141 actgaatgtc taaagacact ttcttttttt gcttttcaag agtttgggat ggacaaattg
    10201 atcatagtaa cacataaaga gaatattgct agtcagaagg ttgctttgaa ggcgcatttc
    10261 aaacaatcta ggagttttaa aggaagcgac cgttatacac gccgtataag agactatatt
    10321 gagtttcaat taactagggg tgattatcgt tatgagtaaa catcaagaaa ttttggagta
    10381 cctagaaaat ttagctgttg gtaagagggt tagtgtacgc agtatttcaa atcatttaaa
    10441 ggtgagtgat ggcactgcat accgcgctat aaaagaggca gaaaataggg gtattgttga
    10501 gactagaccg cgtagtggga cagtacgtgt tgctcaaaaa gcaaaggtta atattgaaaa
    10561 actgacttat gctgaaattg caaggattag tgactcacaa gttgttgcag gaacagaggg
    10621 gctcagcaag gagtttagta aattttctat aggtgcaatg actcacagaa atattgaaaa
    10681 atatttggtg caaggagggt tattgattgt tggcgaccgt gatgaaatac aacatttagc
    10741 cttacaacat caaaacgcta tcttagttac aggtggcttt aatgtttccc catctgtttg
    10801 tcgtttagca gataaacttc aaattcccgt aatggtaaca cactatgata cattcacagt
    10861 atcaacgatg attaatcaca ctttgtcaaa tgccaaaatt cgtactgatt taaagaccgt
    10921 tgaacaagtc tatcagtcgc aaatggatta cggtttctta gcgcaggatg ataccgtaaa
    10981 agaatttaat cttcttgtta aacaaactaa aaatgttcgt tttccaatag tcaaccaagc
    11041 taatgttgtc gttggtgttg ttagtgtgca agatattctt ggaaaagata aagaagtaaa
    11101 attggcaaca gtcatgtcaa aaaatatcat tgttgctaaa cctcgaatga gtctagctaa
    11161 tatcagtcaa aaaatgattt ttgaagattt gaatatgatg ccagtagtat cggatgactt
    11221 tgaattatta ggcgttatta caagacgaca agctgtagaa aatttatcga tgtcgcaagg
    11281 gacagcttta tacacctata gtgatcaaat cttgtctaat ttacaaattg aagatggtca
    11341 cttttcgttt ttagtagaac ccgctatgat tgaccataca ggtagcttaa cacaaggagt
    11401 tttgacggag ttcttaaaag aaatttgtat tcgtgtctta actcgtaagc atcaacgcag
    11461 tattgttgtt aaacaaatga cactttattt cttgcaacct gttcaaattg atgaaataat
    11521 aatggtaacg ccaacaatta tttcagaaaa acggcgcgaa gctacactcg atttagaatt
    11581 aaaattagaa aataaaatta ttgctaaagc tatgatagcg gtaaaaatta attagatata
    11641 aggagaataa atagaaaatg attacattaa agtcagcacg cgaaattgaa gcaatggata
    11701 gagcaggtga ttttttagcc agcatccaca ttggacttcg tgatattatc aaaccaggag
    11761 ttgatatgtg ggaagttgaa gaatacgttc gtagacgttg taaagaagaa aacgtattgc
    11821 cattgcaaat cggggttgat ggtgcagtta tggattaccc atatgctact tgttgtgggc
    11881 ttaatgatga agtggctcat gcctttcctc gtcattatac cctaaaacaa ggtgaccttt
    11941 taaaagtaga tatggtttta agcgaacctt tagctaaatc aattgttgat gtttcatcgt
    12001 taaactttga caacgtggct caaatgaaaa agtacacgga aacatatagt ggtggtttgg
    12061 cagactcatg ttgggcttat gctgtaggtg aagtttcaca agaggtgaaa gacttaatgt
    12121 ctgtgacaag agaagcaatg tatattggga ttgaaaaagc agttatcgga aaccgtattg
    12181 gtgatattgg agctgctatt caggattatg cagagagtcg tggttatgga gttgttcgtg
    12241 atttggttgg tcacggtgtt ggtccaacaa tgcatgaaga gcctatggtg cctaactatg
    12301 gtacagcagg tcgaggtctc cgtcttcgtg aggggatggt tttaaccatc gaaccaatga
    12361 ttaatactgg tacttgggaa attgatacag acatgaaaac tggttgggca cataaaacct
    12421 tagatggtgg tctttcttgt caatatgaac atcaatttgt tatcactaaa gatggtcctg
    12481 ttattttaac gagtcaagga gaagaaagaa cttactaaaa tgaaattaaa aaaattcttt
    12541 gaggatttgc tggctaaact agaatataga cccattcaag tttttatgcg tcattttcaa
    12601 agtgcagaga tggacttatc agccatagca gttgcttatt atctattagt gacagctttt
    12661 ccattactag ttattgctgc taatattttt ccttattttc atattaatgt gtcggattta
    12721 ttgtcactta tgcaaaaaaa tcttccgaaa aatatttatg aacctgcctc acgcttagca
    12781 gtagatgctt tttcgaaacc gtctaccggc attttgggat tcgcgtcatt aacagctttt
    12841 tggacaatgt ctaaaagttt gacctctctt caaaaagcca ttaataaagc atacggtgtt
    12901 gaccaacaca gagactttgt gattagtcgt ttagtgggtg tggggactgg gctgatcatc
    12961 ctatttttac tgacttttgt tttaattttt tcaacctttt caaaaccagt tttacagata
    13021 attgtaaaca tgtatgattt gggagatact ctaacggcgt ggcttttaaa tcttgcacaa
    13081 ccagttacat ttttgactat atttttaggt attggaattt tatactttat tttacctaac
    13141 gcacgaatta gaaaagtacg ttatgtcatt ccagggacat tattttcaac gtttgttatt
    13201 ggttttttta gtaatttaat tagccaatac gtcttaaata gggttgaaaa aatggtagat
    13261 attaaaacgt ttggttcggt cgttatcttt atcttaatgc tatggtttat tttcttagcc
    13321 cacattatga ttttaggtgc aatactgaat gccagcgttc aagaaattgc gacaggaaaa
    13381 attgaatcaa ggcgtggaga tattatgtct cttatccaaa aatccaaaga agaaaaataa
    13441 gaaaagacag ctcttgttag agttgccttt ttttgtcttt aaaaataaca aatttgctca
    13501 agatgtagtt gagtaaaata attaatattt gagatacaag gctttcaatg gtgttgattt
    13561 tatttaaatt gtgctgaacg aattgtccga taatagaagg aaattgatca acaaaaataa
    13621 cagctaatat caaatctaat cctaatgtgc ctaatctagc gatgacaaat gtttgtaagc
    13681 gttgaagctg tccaatttta gtttgtttaa aaacaaatcg atcatttgta aaaaaagcaa
    13741 aaagtatagc tattatgtta gcaactattg cagagaatgg agcgtcctgc gagaaattaa
    13801 aaatcccttg tcgaataact aaataaagaa gggatgttaa tagtccgaac agtaaatata
    13861 agctgacttc attttgaata ctttttttca gtaacgattt taatttaacc atatttttat
    13921 tatatcacgg atgcttcaaa gtgatgagga aatctcccat aagcttggct ttactttttg
    13981 tttgtgatat acttagtaag ttgttttaca caacccttga tgcttagttc cctttcatca
    14041 agcatattac aaacgttaga agtgttaact gtctaaacgt taaaggagaa attatgaaca
    14101 catttacaac tcgcgattac gctcacatgg ctattgtaac agctatctat atcgttttaa
    14161 ccataacgcc accttttaat gctattgctt atggtgctta tcaattccgt gtttcagaaa
    14221 tgctaaattt cttggccttt tatcaccgaa aatacctttt tgcggtaacg ttaggatgta
    14281 tgatttctaa tctttatagt tttggaatga ttgatgtctt tgtaggtggt ggttcaactt
    14341 tactttttgt ttatcttgga accattttat tcaagcaata tcaaaaagat tatctattta
    14401 atggccttat taacaaagca ttctttttct tttctttctt ctttgctgct tcaatgatta
    14461 cggtagcagt tgaattgaaa attgtagcag gattaccttt attattaaca tggttaacga
    14521 cagctgttgg tgaattgact tcacttttgg ttggagcagt ccttgtggat aagttgagta
    14581 gacacgtcga ttttacaaaa taaatatatt ataaagcgcg agacattgat ctcggctttt
    14641 ttgtttggtt ttatagttag catagctaga aaatgctgca acttttatga gaaatgctcc
    14701 aatttttatt aatatcctta gtctttgtga taaaatagaa atatggaaaa tcgtatgaat
    14761 gaattagtat ctttgttgaa tcagtacgca aaagagtatt atacacaaga taatcccaca
    14821 gtttctgaca gtcaatatga ccagttatat cgagagttag tagaattaga agagcaacat
    14881 ccagaaaaca ttttgccaaa tagtcccaca catcgagtgg gtggtttagt tttagaaggc
    14941 tttgaaaaat accaacatga atatcctttg tattcattgc aggatgcttt ctcaaaagaa
    15001 gaattgatag cctttgataa acgtgttaaa gcagagtttc caactgcttc ttatatggca
    15061 gagctgaaaa ttgatggtct atctgtctcg ttgacttatg taaatggtat tttacaggtt
    15121 ggggcaacac gtggtgatgg caatattgga gaaaatatta ctgaaaattt aaagcgtgtt
    15181 catgatattc ctctacattt agaccaaagt ttagatatta ctgttcgcgg agagtgctac
    15241 cttcccaaag aatcttttga agctattaac atagaaaaac gtgcaaatgg tgagcaggaa
    15301 tttgctaatc ctaggaatgc agcagcagga accttgcgcc aattaaacac agggattgtt
    15361 gcgaaacgta aattagcaac ttttctttat caagaagcta gccctacgca aaaagaaaca
    15421 caagatgatg ttttaaaaga gctagaaagc tatggctttt cagtcaatca tcatcgttta
    15481 attagctctt ctatggagaa aatttgggat tttatccaaa ctatagaaaa agatagaatt
    15541 tccctccctt atgatatcga tggtattgtt attaaggtta atagcttagc aatgcaagaa
    15601 gagttgggct tcactgtaaa ggcacctcgt tgggcgattg catataaatt ccctgctgaa
    15661 gaaaaggaag cagaaatatt atctgttgat tggacagttg ggcgaacagg agttgtaaca
    15721 ccaacagcta acctaactcc agttcaacta gcagggacaa ccgttagtag ggccactctg
    15781 cataatgtag attatattgc cgaaaaagat atccgtattg gagatactgt tgttgtatac
    15841 aaagcagggg atatcatccc tgcagtctta aatgtcgtta tgagtaagcg aaaccagcaa
    15901 gaagtcatgc ttattcctaa actatgtcct tcttgtggta gtgaattagt tcattttgaa
    15961 gatgaggtgg ctttacgttg tattaatccg ctgtgcccca accaaatcaa agaacgttta
    16021 gctcattttg ctagccggga tgctatgaat attacaggtt ttggtccatc cttagtcgaa
    16081 aaattatttg atgctcattt aatagcagat gttgcagata tttatcgttt aagtatagag
    16141 gatttattaa ctttagatgg tattaaagag aaatctgcaa ctaagattta tcatgcaatc
    16201 caatcttcaa aagaaaattc tgctgaaaaa cttctttttg gcctaggtat tcgtcatgtg
    16261 ggaagcaagg cgagccgcct tttattggag gaatttggta atttgaggca actaagtcaa
    16321 gcaagtcaag aaagtattgc ctcaattgat ggactaggag gggttattgc caaatcactt
    16381 catacgttct ttgaaaaaga agaggtagat aaactactag aggaattaac ttcctataac
    16441 gttaatttta attatctagg aaaacgagtt agtacagatg cacaactttc aggtttaaca
    16501 gtcgttttaa ccggaaaact tgaaaagatg actcgtaatg aagcaaaaga gaaattacaa
    16561 aaccttggtg ctaaggtgac aggtagcgtt tctaaaaaga ctgatttaat tgttgcagga
    16621 agcgatgcag gaagtaaact aaccaaggct caagatttag ggattacaat acaagatgaa
    16681 gactggttac tcaaccttta agtgaggaag aaatgaagaa acaaaaaaaa gcgcgtttaa
    16741 tttataaccc aacttctggt caagaaatta tgaaaaaaaa tgtagcggaa gttcttgata
    16801 tacttgaagg atttgggtat gaaacatcag cctttcaaac aacgccaact aaaaattctg
    16861 ccagagatga agctgctaga gctgctcaag ctggttttga tttgattgtt gcagcaggtg
    16921 gagatggtac tattaatgaa gttgttaatg gtattgctcc tttgaaacgt cgtcctaaga
    16981 tggcaattat tccaactgga acaactaatg attttgctcg tgccttaaaa ataccgcgag
    17041 gtaatcctat tgaagcaact aaattaattg gtaaaaatca aatcgttaag atggacattg
    17101 gtcaagctca agaggacaat tattttatta atattgcagc tgcaggtagt ttgacagagt
    17161 taacttatag tgtaccaagt caattgaaaa caacgtttgg ctatcttgct tatctagcaa
    17221 aaggtgttga attattacct cgagttcgaa aagtaccagt caaaattact cacgataagg
    17281 gtgaatttat cggtgatgct tcaatgattt ttgttgccat cacaaattct gtaggagggt
    17341 ttgaacaaat tgcacctgat gccaaattgg atgatgggaa atttaccctt attttagtaa
    17401 aaactgcaaa tcttattgag attatgcatc tcattcgttt ggtgctagca ggtggaaaac
    17461 atattaatga taaacgagta gagtatatta aaacgtctta tctaactatc gaacccctat
    17521 cagacgagcg tatgatgatt aacttagatg gagaatatgg aggagatgct cccattactc
    17581 tagcaaacct taaaaatcat attaggttct ttgctaatac tgatgaaatt tcggatgatg
    17641 ctttagtttt agataaagat gaattagcga tagaagctat tgcacagaag tttgctaatg
    17701 aggtaaatga tttagaagga ttaggagatt gatgaatgga taatactgtc attgtacatt
    17761 accatagccg aaagaaaaat tactttaatt taagtttatg gcagtggcgc gatggctcag
    17821 agggtcaaga tgctcatttt tcacggtttg acagtttcgg tgctgttgcg atactaaaat
    17881 atcccttgcc atatttttta agccattatt atgtgattgt taaggataaa aattggcatc
    17941 ataaaacaat agattatcgc attgattgta ctaaaggtgg tgcacgtaaa gaagtttgga
    18001 ttgtagatgg ggatgacaat ctgtattatt ctcgtcaagc agcagttagt agccatcatt
    18061 atagtagacg ccaacctcat gcctacgata tggcaatcaa tagtagagcg tttgataaaa
    18121 aatggggatt tgatggttgg ttaggttttg aatattcaga aactaaaacg gcttttagat
    18181 tgtgggcccc tactgcggaa cgtgtagaac tgattttata tcattcgaca gatgaaacag
    18241 caagtgtatc aaaagttctt tctatgaaaa gagggacagc cgtcaattat aaaaatcata
    18301 aagaaaacac ccatggcgtc tggtttactg aacttgaagg aaactataat taccaagcat
    18361 atacttatcg agtctactat agacgacgta cctttaaaat tacgagagac ccttattcca
    18421 ttgcaacgac tgcaaatggt aaaaggtcta ttgttattgc tccagaagca ttaactccgg
    18481 aagggtttaa aataagtcat ggaaaagaag ctaagtggcg gctcgaaaac cccaatcaag
    18541 cagtcattta cgaaatgcat gtgcgtgatt tttcgatatc tgagacttca ggagttaaga
    18601 cagattatca aggaaaattt aagggattgc atcaaaaaga tacagttaat cagcatggtg
    18661 ataaaacgac ctttgattat gtgcaagact taggagttaa ctatatccaa cttcaaccaa
    18721 ttttcgatca tcatcagacg tttgatgatg atggtcacta tgcttataat tggggatatg
    18781 atcctgaaaa ctacaacgtt ccagaagcaa gcttctctag taatccacat gaacctgcaa
    18841 cgcgtatttt agaattaaaa tctgctatcc aagcgtacca tgatgcaggc attggtgtta
    18901 ttatggatgt tgtctataat catacttttt catcaactga ttctgcattt caactaacgg
    18961 ttccagatta ttattatcga atgaatcaca atggaacatt tcaaaatggt tcaggctgtg
    19021 gtaatgaaac agctagtgaa aaagaaatgt gtcgtaagta tattcttgat tcagttttgt
    19081 attgggtgaa agaatataat atcgatggtt ttcgatttga tttgatggga ctacatgatg
    19141 tagaaacgat gaacattata agaaatgagc taaataaaat cgaccctcgt attttggttt
    19201 atggagaagg atgggacatg ggagctggtc tcacgcctca aaataaagct aaaaaagata
    19261 atgcttatca gatgcctgga attggctttt tcaatgatga tgtaagagac gctgtcaaag
    19321 gtgcggaaat ttacggagaa tttaaaaaag gcttagtttc tggaaacagt acagaagata
    19381 ttgttgcaaa aggcatttta ggaagtgatg agttagtatc atacattgat cctagtcaag
    19441 ttttaaatta tgttgaagca catgacaact acaacttaaa tgatttgcta tgggaattac
    19501 atcctaatga caacgagaaa caacatattt accgagttga agtagctagt gctatgaatc
    19561 tattgatgca agggatggct ttcatgcaat taggtcagga gtttcttcga acaaagtgtt
    19621 atcctacagg tgataaaggt caacttactc aagcagataa agaaagagct atgaattctt
    19681 acaatgcccc agaccaagtt aatcaggtca actgggataa tgttaccttt cataagagta
    19741 ctattaattt tattaggaaa ataattactt tgaagacaaa ctctccttac ttttcttact
    19801 ctagctttga agagattcgc aaacatgttt ttgtagagtc tgctcaatac cattcaggtt
    19861 tcataagttt tacagtcgaa gaacataaga aaataaaaat aattttcaat attaaaggaa
    19921 aacgcttgca ggatcctttc ataggtgata taataatatc aaatcatgaa gaaggaaaag
    19981 cgatagattc attagatgat gagctttctg taatgatcct tgatatcaca aaataacctg
    20041 aaaacattca ggttattttt gtgtatatta aggaatttga tttgaatttg agtttcttat
    20101 attttagtta atttttcgga aatatagagg gtgaaatatt ttcagatgcg tctgtatgag
    20161 gaaaatgcat tcctcttttt ttgtaccatt gaggaaatat ttttgtctta tattgactag
    20221 tagacctgtg aggtgagtag atgtccaata aagatgagtt atatactttt ggaataggtg
    20281 aaaattttca tctccagaat tatttagacg tacatagcga aaatggaagc ttttgtttca
    20341 gagtttgggc tcctaatgct gaaaatgttc aagttattgg agattttaca gattggcgaa
    20401 atcgacctct ccaaatgaac aaaattcaag caggagtttg ggaagctaac agtttggatg
    20461 cgcgcgaagg tgatttatat aaatatttgg ttactcgtaa aggtggtcaa gtagttgaga
    20521 aaatcgatcc tatggcagtt tatatggaaa gacgtcctgg aactgcaagt gttattaaag
    20581 ttcttagaaa taaaaagtgg gaagatggtc tttggatggg aaggcgcaaa cgtctgggtt
    20641 ttcagaagcg tccaattaat atttacgaag ttcatgctgg ttcatggaaa aaagatgatt
    20701 ttggacatcc tatgactttt tctcagttaa aagattactt aatcccctac cttgttgaga
    20761 tgaattatac tcatgttgaa tttatgccct taatggcaca tccgcttgat atgagttggg
    20821 gatatcaatt aatggggtat tttgcttttg aacataccta tggtactcca gaagaatttc
    20881 aagactttgt agaagcatgt cataaaaata atataggagt tcttgtagat tgggttccag
    20941 gacactttat tcagaatgac gatgctttag cctatttcga tggaactgct acttatgagt
    21001 atcaaaatca tgatcgtgcg cataactatc gatggggagc tctgaacttt gatttaggaa
    21061 aaaatcaagt acaatccttt ttgatttcga gtgccttgtt ctggattgaa cattatcata
    21121 ttgatggtat tcgtgttgat gcggttagta atatgttata tctcgattat gatgaaggac
    21181 catgggaagc taaccaattt ggggataatc gtaatttgga aggatatcac tttttaagga
    21241 aacttaacaa agtaattaaa gagcgtcatc ctaatgttat gatgattgct gaagagtcaa
    21301 cagcttctac cccaataact aaagacttag agtctggagg attgggtttt gactttaaat
    21361 ggaatatggg ctggatgaat gatatcctgc gtttttatga agaagatcct ctctataggc
    21421 aatatgattt taatttagtg acttttagtt ttatgtatat attcaatgag aactttgtac
    21481 tagctttttc acatgatgaa gtagtacatg gtaaaaagag tatgatgcat aaaatgtggg
    21541 gtgaccgtta taatcaattt gctggtttac gtaatttata tgcatatcaa atgtgtcatc
    21601 ctgggaaaaa acttctcttt atgggaagtg agtttggtca atttttagaa tggaaatata
    21661 atgatcaact agaatgggaa aacttaaatg atgatatgaa ccaaaagatg caacgctata
    21721 ctaaacaatt gaatcaattt tataaagatc ataagtgcct atggcgtatt gatgattcct
    21781 ttgacgggct agaaattatt gatgcggata ataaatctga aactgtttta tcttttatac
    21841 gaaaagatga taaaggcgat ttgttacttt gtgtctttaa tatgactcct gtagaacgcc
    21901 ctaactttac tattggtgtt ccgcaagcgg gtatttatga agaagtttta aatactgaaa
    21961 tggaagagtt tggaggtgtt tggaaaaacc acaatcctgt aacgaaaaca caagtggcta
    22021 catggaaaga ttatgatcat actttaagtt ttacattacc agcccttggg gcaagtgttt
    22081 ggagaataaa acgtcggctt cgtaaataag tctgcaattt agtaacctca ttttgaaagg
    22141 aaagaataat atgaaaaatg aaatgttagc actcattttc gtcggaggac aaggcactcg
    22201 actcggaaaa ttgactcaga gcattgcgaa acctgctgtt caatttggag gtcgttaccg
    22261 tattattgac tttgcgcttt caaattgtgc gaattcaggt attaataatg ttggagttat
    22321 tactcagtat caaccgctgg aactcaatac tcatattgga aacggatcta gctggggact
    22381 agatggtatc gattcgggag taactgtatt acagccatat tctgcaacag aaggtaatcg
    22441 ctggtttcaa ggtaccagcc acgctatcta tcaaaatatt gactacattg atcgtattaa
    22501 tccagaatat gtactcattc tctctgggga ccacatctat aaaatggact atgatgacat
    22561 gctacagacg cataaagata atttagcaag cctaacagtc gctgttttag atgttccttt
    22621 aaaagaagca agccgttttg gaattatgaa tactgattca aacgaccgta ttgtcgaatt
    22681 tgaagaaaaa cctgaacatc ctaagtcaac aaaagcttca atgggaatat acatttttga
    22741 ctggaagaga ttacggacag ttttaattga tggtgaaaag aacggcatag acatgtcaga
    22801 ctttggtaaa aatgttatac cagcatacct tgaatcgggt gaacgtgttt atacctataa
    22861 ttttgatggg tattggaaag atgttggtac tattgaatcc ctttgggaag ctaatatgga
    22921 atatattgga gaagataaca aattacatag tcgtgatcgt tcatggaaaa tctattctaa
    22981 aaatttgatt gcaccaccta atttcatgac agaagatgca aatgttaaag attctttagt
    23041 tgtagatgga tgctttgttg caggaaatgt tgagcactcc attttatcaa ctaatgtcca
    23101 ggtaaaacca aatgctatta ttaaagactc ctttgttatg agtggcgcga ctatcggtga
    23161 aggtgcaaag ataaatcgag ctattatcgg tgaggatgct gttattggtg atggtgttgt
    23221 gattgatgga agtaaagaag tagaagttat tggttataaa gaagtagcgg gggttccaaa
    23281 tgaagattga taaatatact gcgattttag gaaatactgt tagttaccca gatatgcaca
    23341 gtttgacgga taatcgtccg cttgcttctc taccttttga tggtaagtat cgtttaattg
    23401 attttcaatt atcctcttta gctaatgcag gcgttagaag tatttatggt atttttcgtg
    23461 ggaataatgt tcgctctgtt tttgaccata ttagaagtgg tcgtgagtgg ggcttaggta
    23521 cactgttgag tcactattct ttaggattct atgatacaga cgatgatact cgtacagtca
    23581 atggggatta ttacgattaa attttaactt acttgaggcg ttcgggtagc gaccagacga
    23641 tatatatgag ttgtgatatg ctttgtaata ttgacttaag tcaagtaatc cacttgcata
    23701 atgcaaatgg tgtgcaagcc accgtggttt ataaaaaaat gccgagatca gctattgcag
    23761 aggctaatca agtcttgacc attgatgaat ctgatcatgt agtgagtcta ggtaaagctg
    23821 tagataatga cgataaccaa aaaatgtctg ctgagattta tgttatcgat acaccttggt
    23881 taatcgaaaa aatggaagag gaagctcaaa ataatgaacc acgtaaactc cgcttcttac
    23941 ttcgtgattt aattgttgaa agtaatgctt tggcttttga atacactggt tatttgtcaa
    24001 acatttcttc tattaaatcc tattatgatg ctaacatgga catgttaaca ccaaataaat
    24061 tttattcgct cttcttttca aaccaaaaag tttatactaa ggtgaaaaat gaggaagcta
    24121 cttattttga taaacaatca aatgtctcta attcgcaact agcctatgga agtattatta
    24181 aaggttattt agaccattct atcgtttcta gaaattgtct ccttgaaaaa gggactcgag
    24241 tagtcaatag tattatcttt ccaaaagtaa aaatcggaga aggggcaacc attgaaaata
    24301 ctattattga caaatgcgtt aaggttgcat ccggagttac attgaaaggt agtttagata
    24361 aaccgttagt gattcctaaa ttttcagaga ttaatgagga tattatacaa tgaaaataat
    24421 gtttgttgcg gcagagggtg ctccctttgc taaaacaggt ggtttgggag atgtgattgg
    24481 cgcactccca aaatcactta gtaaaaaagg tcatgatgtc gctgttgtca tgccatatta
    24541 tgatatggta gaccaaaaat ttggagacca aatagaaaac ctcatgtatt tctatacaga
    24601 tgttggctgg cgtcatcaat atgtaggagt taagagactt tctcaagata atgttacgtt
    24661 ctactttatt gataatcaat attattttta tagaggtcat gtctatggtg attgggatga
    24721 tggggagcgt tttgcttatt tccaattagc tgctttagag ttgatggaga aaatagattt
    24781 tattcccgat gtcttacacg tccatgatta tcatacagca atgattcctt tcttattgaa
    24841 agagaagtac cactggattc aggcctataa taatataaga gcagttttta ctattcacaa
    24901 tattgaattt caagggcagt ttggtccaga aatgttagga gatttatttg gagttggcgc
    24961 tgagagatat gaagatggaa ctcttagatg gaataattgt cttaattgga tgaaagcagc
    25021 aattctttac tcagatagag tgacaacggt atcaccaagc tacgcaaatg aaataaaaac
    25081 tccagaattt ggtaaaggtt tagaccaaat tatgcgtatg gaagcaggta aattatctgg
    25141 aatcgtaaat ggtattgaca gcgatttgtt aaatccagaa acagatgctt ttttgccata
    25201 tcatttttca aaaagcaact tagagggcaa aattaaaaat aagttggctt tacaggaaaa
    25261 tttgggcttg cctcaagata aaaatgtacc attaattggt attgtttcac gacttaccga
    25321 tcaaaaagga tttgatatta ttgcttctga attggacaat atgcttcaac aagatattca
    25381 aatggttatt ttaggaacag gctatcacca ttttgaagaa acgttctctt attttgctag
    25441 tcgttaccct gaaaaactat ctgctaatat tacttttgac ttaggattag cgcaacagat
    25501 ttatgcggct tcagatatct tcatgatgcc aagtgctttt gaaccttgtg gtctctcgca
    25561 gatgatggct atgagatatg gtagcttacc cttggtacat gaagttggag gattaaaaga
    25621 tactgtggta gccttcaatc aatttgatgg tagtggtaca ggtttttcat ttaatcattt
    25681 ttcaggctat tggttaatgc aaactctaaa actagcatta gaagtttata atgattatcc
    25741 tgaagcgtgg aaaaagttac aatggcaagc tatgtcaaaa gatttttcat gggatacagc
    25801 atgtgttgct tacgaacaac tgtatcagca actacaataa tcactttatt ttagtgatta
    25861 gttgataagc ttatcgaatc tctatgctaa acgtttctta tgtaaaacca ctttagtata
    25921 gagattttat tatcttataa ggtaaaatat tattaatgta aagagacact atatcaaaaa
    25981 taaagtgttg tcatatctta ataagggatt aaattaaagc taaaaaaact aaaaattgtt
    26041 atttgaaaag gattgtgtaa accatctcaa actatttcat gaaaacgtaa tcaaatgagg
    26101 aaattcattg acatgcacaa gaaataggaa taaaataacg gagtaaatat tttttaaagg
    26161 agaattctat tatgaattta gctattttag cacttggttt cgccgtgatg ggtgtttcaa
    26221 tcggtgaagg aattttggtt gcaaatattg ccaaatcagc ggctcgtcag cctgaaatgt
    26281 ttagcaaact gcaaacattg atgtttacag gtgtggcctt cattgaaggt accttcttcg
    26341 ttctatttgc gtttacattc ttagtaagat agacgacaat ttagaaaaaa ggagggatga
    26401 ggattggaat ctacaagtaa tccaaccgta tcttttttag gcatcgattt tgatttaacc
    26461 atcctagcta tgtctctctt gacaattact attatcttta tcttagtctt ctgggctagt
    26521 cgtaagatga caataaaacc aaaaggtaag cagaatgtct tagaatatgt ctatgagctt
    26581 gttaataata caatctcaca aaatttagga cattatacta aaaattatag tttattgatg
    26641 tttattttat tcagttttgt cttcattgct aacaacttag gattgatgac atcattgaaa
    26701 acacatgagc ataatttttg gacatctccg accgctaatt ttggtgtaga tattacatta
    26761 tcattattag tagcatttat ttgtcatatt gaaggtattc gcaaaaaagg aattggtggt
    26821 tatctcaaag gtttcttatc accaacacca gcaatgcttc cgatgaattt attggaagaa
    26881 gttactaatg ttgcttcatt agcattgcgt ctttttggta atatcttttc tggtgaagtt
    26941 gtcactggat tacttttaca acttgcagtt ttatcaccat ttacaggtcc actagctttt
    27001 gcattgaata tcgtttggac agcattttca atgtttattg gtttcatcca agcgtatgtg
    27061 ttcattatct tgagctcaag ttatattgga cacaaagttc atggtgacga agaagaataa
    27121 gaaaagagag gagaaatatg tcaatactta ttaatagtac aacgattggt gatatcatta
    27181 tcgtatctgg ctctgtgctt ctcttattta tcttaattaa gacttttgca tggaaacaaa
    27241 tcacaggtat ttttgaagcc cgtgaacaaa agatagctaa tgatattgat actgctgaac
    27301 aggcacgtca acaagctgaa gcttttgcaa ctaagcgtga ggaagaacta tctaacgcta
    27361 aaactgaagc taatcaaatc attgataatg ctaaagagac aggtcttgca aaaggtgatc
    27421 aaattatttc agaagcaaaa actgaagctg atcgattaaa agaaaaagca catcaagata
    27481 ttgcccaaaa taaggcagaa gcactagcag atgttaaggg tgaagtagca gatttaacag
    27541 ttttattagc tgaaaaaatt atggtatcaa atcttgataa agaagctcag agtaatctca
    27601 ttgatagcta tattaaaaag ctaggagatg cctaatgaat aaaaagacgc aggctctaat
    27661 tgagcaatat tcaaaaagcc ttgttgaagt tgccattgaa cataaaattg ttgaagaaat
    27721 ccaacaagaa gtagcagctt taattgatat ttttgaaaca agcgagcttg agggtgtcct
    27781 gtcaagtctt gccgtttcac atgatgaaaa acaacatttt gtgaagacat tgcaaacatc
    27841 atgttcaact tatcttgtta atttccttga ggttattgta caaaatgaac gtgaagcact
    27901 tctatatcct attttgaaat cagttgatca ggaacttatc aaagtgaatg gtcaatatcc
    27961 aattcaaatt acaactgcag ttgctttgtc tccagaacaa aaagaacgtc tttttgatat
    28021 tgctaaaact aaattagctt taccaaatgg acagttagtt gagcacattg acccatctat
    28081 tgttggtgga tttgttgtca atgcaaacaa taaagtaatt gatgcaagtg ttcgtaatca
    28141 attacatcaa tttaaaatga aattaaaata gaaagtggtg tgacttttgg caattaatgc
    28201 acaagaaatt agcgctttaa ttaaaaagca aattgaagac ttccagccaa attttgacgt
    28261 cacagaaact ggtattgtta catatatcgg tgatggtatt gctcgtgctc gtgggctaga
    28321 taatgctatg agtggagaac ttcttgaatt ttcaaatggt gcgtacggta tggctcaaaa
    28381 ccttgagtct aatgacgtgg gtatcattat tttgggtgat ttctcagaaa ttcgtgaagg
    28441 tgatgtggta aaacgtactg gtaaaattat ggaagtacca gtaggggaag ctatgattgg
    28501 acgtgtcgtc aatccgcttg gtcaaccagt tgatggacta ggtgaaattg agacgacagc
    28561 aacacgccca gttgaaacac cagcacctgg tgttatgcaa cgtaaatcag tttttgagcc
    28621 tcttcaaaca ggtctcaaag cgattgatgc tttggttcct atcggacgtg ggcaacgcga
    28681 attgattatc ggtgaccgcc aaactggtaa gacgtctgta gcgattgatg caatcttgaa
    28741 tcaaaaaggt caagatatga tctgtatcta tgtggctatc ggtcaaaaag agtctacagt
    28801 acgtacacaa gttgaaactt tgcgtaaata tggtgccctt gattacacaa tcgttgtgac
    28861 agcttcagca tcacaaccat ctccattact tttcattgct ccatatgcag gagtagcaat
    28921 ggctgaggaa ttcatgtata acgggaaaca cgttttgatt gtttatgatg atttatcaaa
    28981 acaagccgta gcttatcgtg aactttcgct tcttcttcgc cgtcctccag gtcgcgaagc
    29041 ttacccaggg gatgttttct atcttcatag tcgtcttttg gaacgttcag ctaaagtatc
    29101 agatgcttta ggtggaggtt cgattacagc gcttccgttc attgaaacac aagcaggtga
    29161 tatctcagca tatattgcta caaacgttat ttcaattact gacggccaaa ttttcttgca
    29221 agaaaatctc tttaactcag gtattcgtcc ggcgattgat gcaggttcat cagtatctcg
    29281 tgttggtggt gcagcgcaaa ttaaagcgat gaagagagtt gcaggtacgc ttcgtctaga
    29341 tcttgcttct tatcgcgaac ttgaggcatt cactcaattt ggttcagacc ttgatgcagc
    29401 aacacaagca aaactaaatc gtggtcgtcg cacagtggaa gtgttaaaac aaccattaca
    29461 caaaccattg ccagttgaga aacaagttgt gattttatat gctctaacac atggattcct
    29521 tgatgatgta cctgtaaatg atattttagc ttttgaagaa gctttgtatg attactttga
    29581 tgctcattat gataatctct tcgaaacaat ccgcacaaca aaagatttac cagaagaagc
    29641 agaattagat gctgctattc aagcatttaa agatcaatca caatttaaat aataggaggg
    29701 ttctaatatg gcaggctctc ttagtgaaat taaagacaag attctttcta cagaaaaaac
    29761 aagtaagatt actagtgcga tgcagatggt ctcatctgcc aagttggtta aatctgaaca
    29821 agctgctaga gattttcaag tgtatgcctc taaaattcgt caaattacta ctaacctctt
    29881 gaagtcagac ttagtatctg gatcagataa ccctatgtta gcttcacgtc cggtgaagaa
    29941 gacaggttat attgttatta cttctgataa gggtttagtt ggtggttaca attcaaaaat
    30001 cttaaaagcg atgatggaca ctataactga ctatcatact gagaatgatg attatgccat
    30061 tatttcaatt ggtagtgttg gttctgattt tttcaaagca cgtggaatga atgtttcttt
    30121 cgaattacgt ggtttagagg accaaccaag ttttgatcaa gttggtaaaa ttattgctca
    30181 agcagttgaa atgtacaaaa atgagttgtt cgatgaatta tatgtttgct acaatcacca
    30241 tgttaacagt ttaactagcc aagtcagaat gcaacaaatg cttcctatca aagaattgga
    30301 tgcagaagag gcttctgagg atagagtgat tactggtttt gaacttgagc caaaccgtga
    30361 agttatattg gagcaattat tgcctcaata tactgagagt ttgatttatg gagctattat
    30421 tgatgctaaa acagcagagc atgcggctgg tatgacagct atgcagactg caaccgataa
    30481 tgctaaaaat gttatcaatg atttaactat tcaatataac cgtgctcgtc aagcagctat
    30541 cacacaggaa attacagaaa ttgttgcagg tgctaatgca cttgaataat taattagtct
    30601 atagaaaaaa tagtgaacaa aggtatttta aatcctttgt atcatatgat aaggagaaat
    30661 acatgagctc aggcaaaatt gctcaggtag ttggacctgt cgttgacgtt gtatttgcaa
    30721 gtggcgacaa acttcctgag attaataatg cattgattgt ctataaaaat ggcgataagt
    30781 cacaaaaagt agtacttgaa gttgctcttg aacttggtga cggcctcgtt cgtacaatag
    30841 ctatggaatc aactgatggg cttacacgtg gtttggaagt attagatact ggtcgcgcaa
    30901 ttagtgtgcc ggttggtaag gatactttgg gtcgtgtctt caacgttctt ggagatgcta
    30961 ttgaccttga agagcctttt gcagaagatg cagaacgtca accaatccat aaaaaagcac
    31021 catcgtttga tgaattatca acatcatcag aaatcttaga aacaggtatt aaagttattg
    31081 acttattagc accttactta aaaggtggta aagtcgggct tttcggtggt gcgggtgttg
    31141 gtaaaaccgt tcttattcaa gaattaatcc acaacatcgc ccaagaacat ggtggtattt
    31201 cagtatttac tggtgtagga gaacgtactc gtgaagggaa tgacctttat tgggaaatga
    31261 aagaatctgg cgttattgaa aaaacggcta tggtctttgg tcaaatgaat gaaccaccag
    31321 gagcacgtat gcgtgtggca cttactggtc ttacaatagc tgaatacttc cgtgatgtag
    31381 aaggacaaga tgtgcttctc ttcattgata atatcttccg tttcacacaa gcggggtcag
    31441 aagtgtcagc gcttttaggt cgtatgcctt cagccgttgg ttatcaacca acacttgcta
    31501 cagaaatggg acaattacaa gagcgtatca cttcaactaa aaaaggttct gttacatcaa
    31561 tccaagctat ttatgtgcca gcagatgact atactgaccc tgcgccagct acagcctttg
    31621 ctcacttaga ttcaacaact aaccttgaac gtaaattgac acaaatgggg atttatccag
    31681 ccgttgaccc attagcttca agttctcgtg cactcacacc agaaatcgtt ggagatgagc
    31741 actatgaagt tgcaacagaa gtacagcgtg ttcttcaacg ttaccgtgag ttacaagata
    31801 tcattgcgat tttaggtatg gatgaattat cagatgagga aaaaacgtta gttggacgtg
    31861 cacgtcgtat ccaattcttc ctttctcaaa acttcaatgt tgcagaaaca tttactggtc
    31921 aaccaggttc atatgttccg gttgaagaaa ctgttcgcgg attcaaagaa atcctagatg
    31981 gtaaacatga tcaaattcca gaagatgcct tccgtatggt tggtggtatt gaggatgtta
    32041 tcgcaaaagc agaaaaaatg aactattaat gaggtgatgc tatggcacaa ttgactgttc
    32101 aagtagtaac atcagatggt attagatatg accatcacgc tagtctcatt actgttagaa
    32161 cgccagatgg tgaaatggga attttaccag gtcacataaa tttgattgct ccccttattg
    32221 ttcatcagat gaagattaat cgttctcatc aagaaggtgt agactgggta gctgttaatg
    32281 gtggtataat tgaagttaat gaggatcagg tgacaattgt tgcggattca gcagaacgag
    32341 cacgtgatat agaccttaac cgtgcagaac gggctaagga acgcgctgag cgtgcattag
    32401 aaaaagctca aacaactcag aatattgatg agatgcgtag agctgaggtt gctcttcgtc
    32461 gcgcaattaa ccgtattagc gtcggtaaaa agtagaaaaa tggtctaatt agttttgtga
    32521 ctaattaggc ttttttagtg tataatagaa ggcatgaata tgatattaaa tatagtaact
    32581 atagttagta attttatttt tattgccctt ttttatcaac tttttgtaga tttatttgac
    32641 tggtctaaaa tgattaaaat gtcccctcaa aacatctcaa aattaaaagt ttttattttg
    32701 ttgataagta tagttggtgg ctatgtggtt agtcattttt tattggaagt gattcagttg
    32761 tgtcaatcaa tcttttgggc tctccaataa aggaaatatt agctgaacta tgatataatc
    32821 aacttactat tgttataaga atggagattc aaatggataa aattattgtg gaaggtgggc
    32881 aaacgcggct tcaaggtcaa gttgttattg aaggagctaa aaatgcagtt ttacctcttt
    32941 tagcagccac aattttacca agccaaggga aaacattatt gaccaatgtt cctatcttgt
    33001 ctgatgtatt tacaatgaac aatgttgttc gtggtttaga tattcaagtt gactttaatt
    33061 gcgataaaaa agaaatctta gtggatgcct caggtgatat tttagatgtt gcaccttatg
    33121 aatttgttag tcaaatgaga gcttctattg ttgttttggg acctatttta gctagaaatg
    33181 gccatgctaa ggtttcaatg ccaggcggtt gtacaattgg tagccgacca attgaccttc
    33241 atcttaaagg tttagaagct atgggagcta cgattacaca aaatggtggt gatattactg
    33301 cccaagctga aaagcttaag ggggctaata tatacatgga ttttccctca gtcggtgcca
    33361 ctcaaaactt gatgatggct gctacacttg cttcaggcac aacaacgata gagaatgctg
    33421 cacgtgaacc agaaattgta gaccttgcgc agttactaaa taaaatggga gctaaagtca
    33481 agggtgctgg tacggaaaca ttaacgatca ttggtgttga tgctttacat ggtacagaac
    33541 atgatgttgt ccaagatcgt attgaagctg gaacctttat ggtggcagct gctatgactt
    33601 ctggaaatgt tctagtaaag gatgctattt gggaacataa ccgtccttta atctctaaat
    33661 tgatggagat gggtgttgaa gtgtcagagg aagaagatgg tattcgtgta aaagctgata
    33721 ctaaaaaatt gaagccagtg actgtgaaaa cactccctca tccaggattt cctactgata
    33781 tgcaagctca atttacagct ctaatggcag tagttaatgg cgaatcaact atgattgaga
    33841 cagtttttga aaatcgtttt caacatttgg aagaaatgcg tcgcatggga ttacaaacgg
    33901 aaattttacg agatactgca atgattcatg gtgggagagc cttacaaggt gcaccggtca
    33961 tgtctactga cttacgagct agtgcagctt taatcttggc aggaatggtt gcgcaagggc
    34021 aaacagtagt tggccaatta acccatttag accgtggtta ttatcaattc catgaaaaat
    34081 tagcggctct tggtgctaat attaaacgtg taagtgaggc gtaatatggc atctagtggt
    34141 tggaaatatg tactaaaaca aatcggtcta atagtattag tcatactgtt agccttgctg
    34201 tttttagcgg ttggattgat gttgggttat agtgtttttg gtgacggaga gcatgcttac
    34261 tctattttat cgctggataa atggcaaaat attattggaa aatttttagg aaaatagaaa
    34321 gagccactat aagttatttg atgcttatag tggtttcctt tgagagttaa ttttagcagt
    34381 agaataatac aatgacaaaa aaacaaaaac aaataacagc ttcgattgta actttgcttt
    34441 tttcattact ggcaataggg ttaggtgaaa gtaaatcttt gcctaatgat cacattttaa
    34501 aacaggtgac agatttaatt attagtaatg aatctggcaa aaatacttcc cttaaaaata
    34561 tatcaggaac ccctagtaga gagttatctg aatctgtttt gactagcaat gttaaaaaac
    34621 aattgggaac aaatatagct tggaaccagt caggtgcttt tatcattaat caaaataaaa
    34681 cagatttgaa tgctaaagtg tcaagtgcac catatgctat taatgaaata aagaaggtta
    34741 ataaccaaat tgttccaact aaagcaaatg ccttattaac aaaagcaact cgtcaatatc
    34801 gaaatcgaga agaaacaggg aatggtagaa cttattggaa accagctgga tggcatcaga
    34861 taaatggatt aaaagggagc tataaccacg ctgtggacag aggccattta attggttatg
    34921 ctttagtagg gagtttaaga ggatttgatg cctccacaag taatcctaaa aatattgcca
    34981 cacaagcagc ttgggcaaac caagctaata gcaaccaatc aacagggcaa aattattatg
    35041 aaactttagt tcgtaaagca ttggatcgtc ataaaactgt taggtatcgt gtgactttaa
    35101 tttatgacag agataatctt ttatcttcgg gatcacatat tgaggctaaa tcttcagatg
    35161 gaagtttaga atttaatgtt tttattccta atgtacaatc gggattatta tttgattatg
    35221 caacaggaaa agtgaagcag acaaagtaat ctttatctaa tagtgcttgt taaagactga
    35281 aaaatttgat aaaatagtcc ttaattgaat agaggtctgt gagactagta aaccaaggga
    35341 agctgcttag ggagttcagc cgtgactgca agctgaatca gtgaaagagg gtggaattca
    35401 ctcacaaata ggactataat taaggtctga ttggtcgatc agatagaaaa cggatggtac
    35461 cgcgtgtcaa cgctccgatt aagggggagt ggcacgcttt ttttgttttc aaagctactt
    35521 attttgactt ggaggaaaga tggatttaca aaaacaatta gaagagttga aaatatcaac
    35581 tcaagaaaag cttaaagaaa tgactgggaa ccacactaaa gaattacaag acttacgtgt
    35641 acaagtttta ggaaaaaaag gttctctaac agaattacta aaaggtctga aagatttatc
    35701 taatgattta cgaccagttg ttggaaaaca agtcaatgaa gtacgtgata ttttaacaaa
    35761 agcgtttgag gaacaagcta aagtagtgga agctgcgaag attcaagcac agttagagtc
    35821 tgagagtgtc gacgttacct tgcctggtcg tcaaatgaca ttaggtcacc gtcatgtctt
    35881 aacacaaact agcgaagaga ttgaagatat ctttttaggt atgggattcc aagtggttga
    35941 tggttttgaa gttgagaaag actactataa ttttgaacga atgaatttac caaaagatca
    36001 tccagcccgt gatatgcaag atacttttta tattacagaa gaaatcttgc tacgtactca
    36061 tacttcgcct gttcaagcac gaacaatgga ccaacatgat ttttctaaag ggcctttaaa
    36121 aatgatttct cctggacgtg ttttccgtcg tgatactgat gatgctactc atagccatca
    36181 attccatcaa attgaaggct tagttgttgg agaaaatatt tcaatgggcg atctcaaagg
    36241 aacacttcaa ctgatttctc aaaaaatgtt tggagcagag cgtaaaatcc gacttcgtcc
    36301 ttcatatttc ccattcacag aaccttcagt tgaagttgat gtctcttgct tcaagtgcgg
    36361 aggtaaggga tgtaatgtct gcaaacagac aggttggatt gagatattgg gtgctggtat
    36421 ggttcaccca agtgttttgg aaatgtctgg tattgattct gagaaatact ccggttttgc
    36481 ctttggctta ggtcaagaac gtattgcaat gttacgctac ggtatcaatg acattcgtgg
    36541 cttctatcaa ggtgatgttc gcttcacaga tcaattttaa gattttagat agcaagtagc
    36601 atcattggtg tgatgataaa tttatgttaa cgctattacc ttactcaata ggaggttttc
    36661 taatgtctca aattactttt aaaaaggttg gccttgataa tgtgaatatt ttacagaatc
    36721 ttgctataga aacctttcgg cagacgtttt ctcatgataa tagtgaggaa caacttcaag
    36781 catttttcaa tgagtcttac actttaccag tattaaaatc ggaaattacc catgctgaat
    36841 ctgatactta ttttgtttat cttgatacag atttagtggg ttatttaaaa gtaaactggg
    36901 gaagtcagca aactgaaaaa aacttgataa agcatttgaa attcaaagga tttatcttct
    36961 agatgcctac caagggaaag gtataggtaa ggctactttc gaatttgctt tggatttagc
    37021 ttataaatca ggcttagatt gggcttggtt aggtgtttgg gagtttaatc ataaagctca
    37081 ggcattttac gctaaatacg gatttgaaaa attttctgag caccaatttt cagtaggtga
    37141 taaagtggat acggattggt tattaagaaa aagtttacac taaaaactac aaagcacttg
    37201 tataaaaata aaagaaaata aatagagaga aattacatgt tagtatcgta taaatggtta
    37261 aaagagttag tggatgttga tgtcacaaca gcagaactag ctgagaaaat gtcaaccact
    37321 ggtattgagg ttgaaggtgt ggagacacca gcggaaggtt tatcaaaatt agttgtaggt
    37381 catattgttt cgtgtgaaga tgttccagat acccacttac atttgtgtca agttgataca
    37441 ggggacgatg aacttcgtca agttgtctgt ggtgctccaa atgtcaagac tggtattaat
    37501 gttatcgtag cggttcctgg tgctcgtatt gcagataact ataagattaa aaaaggtaaa
    37561 atccgtggta tggagtcact tggaatgatt tgttcacttc aagagttggg cttatcggaa
    37621 agtatcattc caaaagagtt ttctgatggt atccaaattt tacctgaggg agctatacct
    37681 ggtgatagta ttttctctta tcttgactta gatgatgaaa ttattgaatt atcaattaca
    37741 ccaaaccgtg cagatgctct ttcaatgcgt ggtgttgccc atgaggtagc agcaatttat
    37801 gggaaaaaag ttcattttga agaaaaaaat ctcattgaag aagcagaaag agcagctgat
    37861 aagatttctg ttgtcattga atcagataaa gttttatctt actcggctcg tattgttaag
    37921 aatgtaacag ttgcaccaag tccacaatgg cttcaaaata aactgatgaa tgctggtata
    37981 cgtccaatta ataatgttgt tgatgtgaca aactatgttc ttttgactta tggtcaacca
    38041 atgcatgctt ttgattttga taaatttgat gggacaacta tagtagcacg taatgctgag
    38101 aatggtgaaa agttaattac tcttgatggt gaagagcgtg acttaatagc tgatgacttg
    38161 gtcattgcgg taaatgatca accagttgct ttggcaggcg ttatgggagg acaatcaact
    38221 gaaattgata gttcttctaa gacagtggtc cttgaagcgg cagtatttaa tggaacaagt
    38281 atccgcaaaa caagtggaag actcaacctt cgttcagagt catcttctcg ctttgaaaaa
    38341 ggtatcaatt atgatacggt ttcagaggct atggattttg cggctgcaat gttacaagaa
    38401 ttggcaggca gtcaagttct ttctgggcaa gttacagaag gagttcttcc aactgagccg
    38461 gtagaggtat caacgactct tggttacgtt aatactcgtt tgggaacaga acttacctat
    38521 actgatattg aagaagtatt tgaaaaactc ggctttgcta tttctggttc agaagtgaaa
    38581 tttactgtgt tggtaccgcg tcgtagatgg gatattgcca ttcaagctga cttagttgaa
    38641 gagattgccc gtatttatgg ttacgaaaaa ttaccaacaa cattgccaga agccggagcg
    38701 actgcaggag aattaacgag tatgcaacgc ttacgtcgta gagtccgtac ggttgctgaa
    38761 ggagtaggat tatctgaaat tatcacctat gctttaacga ctccagaaaa agcagtgcag
    38821 ttctcaactc aagcaactaa tataactgaa cttatgtggc cgatgactgt tgatcgctca
    38881 gcacttcgcc aaaatgttgt ttcaggaatg cttgatacaa ttgcttataa cgttgctcgc
    38941 aaaaacagta accttgcagt ttatgagata ggaaaagttt ttgaacaaac tggtaatcca
    39001 aaagaagatt taccaactga agtagagacg tttacttttg ccttaacagg tctagtagag
    39061 gaaaaagatt tccaaacaaa agccaagcca gttgatttct tctacgccaa aggtattgta
    39121 gaggcgctct ttattaaatt aaaattagat gttacttttg tagcacaaaa aggtttagca
    39181 agtatgcacc caggtcgtac agcaactatc ttattagatg ggaaagagat tggttttgtt
    39241 ggtcaagtac acccacaaac tgcaaaacaa tatgatattc ctgaaactta tgttgctgaa
    39301 atcaatctat caactattga aagtcaaatg aaccaagcgt tgatttttga agatattacg
    39361 aagtatccgt ctgtatctcg cgatattgca ttattacttg cagaatctgt tagtcatcat
    39421 gatattgtgt tagcaattga gtcatcaggg gtaaaacgtc taacagcgat taaactgttt
    39481 gatgtctacg ctggaaataa tattgcagaa gggtataaat caatggctta tagcttaact
    39541 ttccaaaacc ctaatgacaa tctaacggac gaagaagtgg ctaaatatat ggaaaaaatc
    39601 actaaatcac tcgttgagaa agttaatgca gaaataagat aatttatatc ttacgttgaa
    39661 aaataaataa aaaacagcat caattaagat gctgtttttt tatttaaaat ttatagatgt
    39721 tctgcggaga aagtatcacc gtgttggatg tcaccatatt gaaagccttt gttaaaccag
    39781 cgttggcgtt gttgagctgt tccatgggta aagctatcag gcactaattt tccgtaggtt
    39841 tctttctgaa gggtatcgtc tccgacggcg tgggcagcat tcatggcctc ttcaaagtct
    39901 ccttgttcta agagattttt tcccctgatg tagtgagccc atacccctgc ataataatct
    39961 gcttgaagtt ctagccgaac atttaaagca tttgcttctt tcttagtaag tccgtgtcgc
    40021 attctattat acttatccat aatgcctaac tctgtttgaa tgtggtgacc aacttcatgg
    40081 gcgatgacgt aggccatagc aaaatcacca gtagcaccat atttatgtga taattcattg
    40141 taaaaagaaa tatcaagata gattttttta tctgctgaac aataaaatgg ttctgaagca
    40201 gattcaccta taccacaacc tgtttgaatc gaattggtgt aaagaacaag ttttggttcc
    40261 ttataatttc caaaaccttg ggtttggaat tcttgtgacc agaaatcctc agttgatcca
    40321 aggactttat taacgaaatc gatttgttct ctcgttgcgc tattatcaac agaacgtgag
    40381 acattctgag attggtaact agaaggtgag gatgagtcat taaaaatgct ggttagtccc
    40441 cctccgccaa gtagcagtaa gatgattaaa accacaagct tggttttcca actccctcgc
    40501 atcaataaaa gttgaagaat cggtaatcca cttcctccgc tagagaatga acctccgcta
    40561 ctggagcgac gatcttcaac attgtcgctt tttcttaggt catctatttt cataagtatc
    40621 tccaaagtgt tttataggct cattataaac tattttaata agaaatgctt gaatttttaa
    40681 ctaaatatct tcccctagat ttgaagctat ttttggtata atattaataa actgtaaaga
    40741 ataagttgaa gaaggtttat ttctcagttg acttataaga taggagattc atgaaattac
    40801 tttatactga tattaatcat gatatgacag aaatcttagt caatcaggcc gcgcatgctg
    40861 ctgaagctgg atggcgtatt ttttatattg ctccaaactc tttatctttt gaaaaggagc
    40921 gtgcagtatt agagaccctt cctcaagaag cctcttttgc tattacaatt actcgctttg
    40981 ctcaattggc acgttatttt actttaaacc agcctaatca aaaagaaagt ttaaacgata
    41041 ttggattagc aatgatcttt tatcgtgctt tagcaagttt tgaagatgga caattaaaag
    41101 tttttggacg attgaagcaa gatgcttcct ttatcagtca attggtagac ctatacaaag
    41161 aattgcagac agcaaatctg agtattttag atttaaaata tttacattct cctgaaaaat
    41221 ttgaagatct attagctatt tttctagtgg tttcagatct tttaagagaa ggagaatacg
    41281 acaatcaatc taaaattgcc ttttttacag aacaagtaag gtcaggacag ctagatgtgg
    41341 atttaaagaa cactatcctt atagtcgatg gttttacccg tttctcagct gaagaagaag
    41401 ctttaattaa aagtctgagt tcacgttgtc aagagattat catcggagct tatgctagcc
    41461 aaaaagcgta taagactaac tttaccaatg gaaatattta ttctgcaggg gttgattttt
    41521 taagatatct agcaacgaca tttcaaacaa aaccagaatt tatcttgtct aagtgggaaa
    41581 gtaagtctgg ttttgaaatg atttcgaaaa atatcgaagg taaacatgat tttacaaata
    41641 gtagtcacat tttagacgac actgctaaag attgtataac aatttgggag tgcattaatc
    41701 aaaaagatga agtggaacat gtggctagag ctatacgtca gaaactttat cagggatatc
    41761 gctacaaaga tattttagtt ttactaggag atgtagatag ttataaacta caattaagta
    41821 agatttttga acagtatgat attccttatt atttcggtaa agctgaaaca atggctgcgc
    41881 accctttagt tcattttatg gattccttgt cgcgaataaa acgttatcga tttagagctg
    41941 aagatgtttt aaatcttttt aagacaggta tttatggaga gattggtcaa gatgatttag
    42001 attattttga agcttatatc tcatatgcag atattaaagg tcctaagaag ttctttacag
    42061 attttgttgt gggggctaaa aaatttgatt tagggcgtct taacactatt cgtcagtccc
    42121 tccttactcc actggaaagt tttgttaaaa cgaaaaaaca agacggtata aaaacactca
    42181 accaattcat gtttttcctt actcaggtag gtttgagtga caatttatcg cgattagttg
    42241 gtcaaatgtc tgaaaatgaa caagaaaaac atcaagaagt ttggaaaaca tttacagata
    42301 ttttagagca gtttcaaaca atatttggtc aagaaaaatt aaatttagac gaatttttat
    42361 cgttattaaa ttcaggtatg atgcaagccg agtatcgtat ggtccctgct actgttgatg
    42421 tggttactgt taagtcttac gacttggtag aaccacatag taatcaattt gtctatgccc
    42481 taggcatgac acaatcacat tttccaaaga ttgctcaaaa caaatcttta atatctgata
    42541 ttgaacgtca gcttataaat gatgccaatg acactgatgg tcattttgac attatgacgc
    42601 gagaaaattt gaagaaaaac cattttgctg ctctctctct ttttaatgct gctaaacaag
    42661 cattagtgtt gactatacca caattattga atgagtctga agatcagatg tcaccatatc
    42721 ttgtcgaact aagagatata ggtgttcctt ttaatcataa aggacgccaa tcgttaaaag
    42781 aagaagctga taatattggg aattacaaag ctttattatc gcgtgttgtt gacttgtatc
    42841 gctctgctat tgacaaagag atgactaaag aagaacaaac tttttggtca gttgctgttc
    42901 gttatttgcg tcgtcaatta acttctaaag ggatcgagat accaataatt acagatagtc
    42961 ttgatactgt gacagtttca tcagatgtga tgaccagacg cttcccagaa gatcacccac
    43021 ttaaattatc ctcatctgct ttgacaactt tttataataa tcaatataag tattttttac
    43081 aatatgtgtt aggtttggaa gaacaagact caattcatcc agatatgcgg caccacggaa
    43141 cgtatcttca tcgtgttttt gaaattctga tgaaaaacca agggatagaa tcttttgaag
    43201 agaaactcaa ctctgcaatt aataaaacca atcaagaaga tgttttcaaa tccttgtatt
    43261 cagaagatgc agaaagtcgt tattctttag aaattttaga agatatcgcg cgtgcaactg
    43321 caaccatatt acgacaagat agtcagatga ctgttgaaag tgaagaagaa cgatttgaac
    43381 taatgattga taatactata aaaattaacg gtattattga ccgtatcgat cgtttatctg
    43441 atggttcttt gggtgtcgtg gactataaat caagcgctca aaaattcgat atccaaaaat
    43501 tttataacgg tctaagtcca caattagtca cctacattga tgctatcagt cgtgacaagg
    43561 aagttgaaca aaaaccaccg atttttggag ctatgtactt acatatgcaa gaacctagac
    43621 aagacttgtc taaaattaaa aatttagatg atttagtaac aaaaaatcat caagctttaa
    43681 cttataaagg attattttca gaagccgaaa aagaattctt agcaaatggg aaataccatc
    43741 tcaaagattc cctttattca gaggctgaaa ttgctatttt acaagcacat aatcaatcgc
    43801 tttacaaaaa agcatctgag actattaagt caggtaagtt tttgatcaat ccatatacag
    43861 aagatgctaa aacagttgac ggtgatcagt ttaagagtat tacggggttc gaggcggata
    43921 ggcatatggc acgtgccaga gcgctttata aattacctgc taaggaaaaa cgtcaaggtt
    43981 tcttaacatt aatgcagcaa gaggaggaaa atgatgacct ttaagccttt cttaaatcca
    44041 gaagatattg ctgttattca gacagaagaa aaaaattctg ataaaaaaca aaagcgaacg
    44101 cctgaacaaa ttgaagcaat ttatactttt ggtaacaatg tcctggtatc agcttcggct
    44161 ggttctggaa agacttttgt aatggttgag cggattttag ataaactttt aagaggggtt
    44221 ccgattgagt ctttgtttat ctcaacattt acagtaaaag cggcaggtga gctcaaagaa
    44281 cgtttagaaa agaaaataaa cgagtctttg aaatccgcag aatcagatga tttaaaacag
    44341 tttctcaccc agcaacttgt cggcattcaa acagctgata ttggtactat ggatgctttt
    44401 acgcaaaaaa tagttaacca atatggttat actttgggca tatcacctat atttaggatt
    44461 ttacaagata aaaatgagca agacgtcata aagaatgagg tctatgctga tttattttct
    44521 gactacatga ctgggaagaa tgcagcttca ttcataaaat tagtcaaaaa tttctcaggt
    44581 aatcgtaaag atagcaaagc ttttcgagaa atggtttata aagtttatgc ttttagccag
    44641 tccatcgata accccaagcg ctggatgcaa acagtttttc taaaaggtgc acaaacttac
    44701 actgattttg aagctattcc agatcaagaa gtttcaagcc ttttaaatgt aatgcaaaca
    44761 actgctaatc aattacgtga tttaacggat caagaagatt ataagcaatt aactgctaaa
    44821 ggggttccga cagcaaatta caaaaaacac cttaaaataa ttgaaaattt agtccattgg
    44881 tctcaagact ttaatttgct gtatggaaaa aaaggattgg ctaatttggc acgtgatatc
    44941 actaatgtga ttccttcagg caatgatgtt acagttgctg gcgttaaata tcctatcttt
    45001 aaacaattgc ataatcgtat cgttggactt aagcacttag aagttatttt taagtatcaa
    45061 ggagaatctc ttttcttgtt agaattattg caatcctttg ttttagattt ctcagaacaa
    45121 tatttacagg agaagattca agaaaatgct tttgaatttt ctgatattgc ccattttgca
    45181 attcaaatat tggaagaaaa tcatgatatt aggcaaattt atcaagataa gtatcatgaa
    45241 gtaatggttg atgagtatca ggacaataac catacccaag agagaatatt agaattattg
    45301 tctaatggac ataatcgttt catggttgga gacataaaac aatcaattta ccgttttcgt
    45361 caggctgatc cacaaatttt taatgataaa tacaaagctt accaagacaa tccaagtcaa
    45421 gggaaattaa ttattctgaa agagaatttt cggagtcaat cagaggtttt agacagtaca
    45481 aatagtgtgt ttacacatct tatggatgaa gaagtaggag acattcttta tgatgaaagt
    45541 caccaattaa aagctggtag tccaagacag caagaaagac atccgaataa taaaacacaa
    45601 gttttactat tagatactga tgaagatgat atagatgaca gtgatagtca acaatatgat
    45661 attagcccag ctgaagcaaa gttagtcgca aaagaaatta ttcgattgca taaagaagag
    45721 aatgttccct ttcaagatat caccctctta gtttcttcaa ggacaaggaa tgatggtatc
    45781 ttacaaactt ttgaccgtta tggtattcct ttggtaactg acggtgggga gcaaaattac
    45841 ttaaaatcag tcgaagtgat ggtaatgctt gatactttgc gatcaattga taatccctta
    45901 aatgattatg ctctagttgc tcttttgaga tctcccatgt ttggctttaa tgaagatgat
    45961 ttaacaagaa ttgctattca tgatgtcaaa atggcttttt atcataaagt gaaattgtct
    46021 tatcataaag aaggtcatca tagtgattta attactccag aattatcttc taaaatagat
    46081 tactttatga agacattcca gacttggcgt gattttgcta aatggcattc gctttatgat
    46141 ttaatatgga aaatatataa tgaccgtttt tattatgatt atgtaggagc tctgcctaaa
    46201 gctgagcaac gtcaagccaa tctatatgcc ttggcacttc gagcaaatca gtttgaaaaa
    46261 acaggtttca agggtttatc acgttttatt cgaatgattg ataaagtttt agaaaatgaa
    46321 aatgatttgg cagatgttga ggtggcatta ccgcaaaatg ctgttaacct aatgactata
    46381 cacaaatcaa agggtcttga gtttaagtat gtatttattt taaatattga taagaaattt
    46441 tcaatggtag acataacttc ccctcttatt ttaagccgaa atcaaggaat tggtattaaa
    46501 tatgttgctg atatgcgaca tgaactcgaa gaagagatac tgcctgcagt aaaggttagc
    46561 atggaaacac ttccttacca acttaataaa agagagttac gcttagctac tttatcagag
    46621 caaatgcgtt tactttacgt agctatgaca cgtgcagaga aaaaattata tctggttggt
    46681 aaggctagtc aaaccaaatg ggctgatcat tatgacttag tatctgaaaa taattatctc
    46741 ccattagcta gtcgtgagac ttttgtgacc tttcaagatt ggttactggc tgttcatgaa
    46801 acctataaga aacaagagtt gttttatgac atcaacttcg tatcattaga ggaattaaca
    46861 gatcaccaca tagggatggt taatcctagt ctacctttca atcccgataa taaggcagag
    46921 aatcgtcagt ctgaagatat cgtgcacgcg attagcgtgt tagaatctgt tgaacagatt
    46981 aatcaaactt ataaagcggc tattgagtta ccgacagtaa gaacaccaag tcaagtcaaa
    47041 aaattctatg agcctattct agatatagaa ggcgttgatg tgatggaaac tattactaaa
    47101 acatcagtag attttaagtt acctgatttt tctacaagta aaaagcaaga cccagcagct
    47161 ttaggatctg ctgtacatga attaatgcaa cgcatagaga tgtcatctca tgtcaaaatg
    47221 gaagatattc aaaaagcctt gacagaagtg aatgctgaaa caagtatcaa agcggctatt
    47281 cagattgaaa aaatcaacta ttttttccaa gaaaactcac tcggtaaata tatacaagaa
    47341 gaagtagaac atctacatcg agaggcacct tttgccatgt taaaagaaga tccggagagt
    47401 ggtgagaaat tcgttgtacg tggtattatt gatgggtatc tactattaga aaatcggata
    47461 attctatttg attataagac tgataaattt gtcaatcctc tggaattaaa agaacgctat
    47521 caggggcaaa tggctttata tgcagaagct ttaaaaaaat cttacgagat agaaaggata
    47581 gacaaatact taattctttt aggtggtaaa cagctagaag tggtaaaaat ggattaaaga
    47641 aaggaaaaaa tgatagtcga acaaaaattt ggaaatggtt ttacttggat taacattgaa
    47701 gcagagcaat tgcgaacaga aacatcggaa attcaagcaa aatatttgga tagtgagatt
    47761 attacatatg ctctggatga ttatgaacga gcctttatgg aatgttctca tattaaagga
    47821 aaagaagtct tgacgattat ttttaatact attgatctaa agcagaaaga aagttattat
    47881 gagaccgttc cgatgacctt ttgcttatca cacgaccgtt tgataacggt gacacggtct
    47941 cgaaatagtt atatgctaga actattgcaa aaatatctag accgtaatcc agatgtctct
    48001 cctaaaaaat ttttatttgc agctttaact ttgattacaa aacaatattt taatgttgta
    48061 tcgaaaattg atagagaaaa ggatattctc aaccgtcaat tgagagaaca aacaaccaat
    48121 aagcgcttat tagccatgag tgacctagag acaggctccg tttatctttt gacagctgcc
    48181 aatcaaaatg cattggtatt agagcaatta gatgttcatc ctagtcagcg atttaattct
    48241 gaagtggaaa aagaacagtt atcagatgct ctcattgaag cgcatcagtt agtcagtatg
    48301 acacaattaa acagtcaggt actttcacaa ttatcatcta cttttaataa tgtcttaaat
    48361 aataacttga acgaaaactt aacaggtttg aatatcattt cgattaattt agcgattatt
    48421 gcagcaatta ctggcttttt tgggatgaat attccattac ctcttacaga atcacggagt
    48481 tcatggttaa ttgttattgc tacttcggtc ttactttggg ttattattgc tcaaatttta
    48541 aaacgagttt ttaacttaag aaaataaaaa tgttcagtgc gtaagcaccg aacatttttt
    48601 atttacctaa gcagaattgg ctgaagagct gagttattag ctcatctgga gctgcatccc
    48661 cagtgatttc tccaagtatt tcccaagttc tagtcatatc gacttgtaag agatcaacag
    48721 gcattcctaa ttccaagccc tcattgacag cttttaaact atcaacggct ttttcaatta
    48781 gtgagatatg gcgagcattc gatagataag tagcgtcatg ttctaccata ccagcattgt
    48841 caaagaaaat atcgttaata cgttcttcga ttttatcgat gttttcattt tctaaaaccg
    48901 aaattggaat aacattttca ggtaattcgt taacttctat tttttgagga agatccgttt
    48961 tattgagtaa tacgatacga ttactttctt tacttagttc tagaagggag cggtcttgta
    49021 atgttagtgg ttcggaacta ttgagaacca acaacacaag gtcggcttct tctaaagcct
    49081 tctttgaacg ttcaacacca attttttcaa cgatatcatc tgtatcacgg ataccagcag
    49141 tgtcaactag ttttaaagga actcctttaa tattgacgta ttcttcaata acatcacgag
    49201 ttgttccttc aatatctgtc acaatcgcct tttcttcacg taagaggtta tttagtaagc
    49261 tggatttacc gacatttgga cgaccaatga ttgctgttga aagtccttct cgaagaattt
    49321 ttccatgtcg ggcagttcga agtaaatttt ccataagtgc ttgaaactct tgtgtttttt
    49381 cacgcatcag tgttgtagtc atctcttcaa cgtcatcata ttcagggtaa tcgatattaa
    49441 cttcaacttg ggctagagta tttagaattt cttgtcgagt gttattgatt agtgtcttta
    49501 aagaaccatc taattgctta acggcaatat ccattgcttt atctgtttta gctctaataa
    49561 gatccataac agcttcagcc tgagtcaagt caactcgacc gtttaaaaaa gcacgtttag
    49621 taaattctcc tggttcagcc attcgtgctc catgtcgtaa aattaactga agaatttcgt
    49681 tagtaactgc aattcctcca tgcgtattaa tttcaataac atcttctctt gtgaatgttt
    49741 ttggagcaag cataacgcct aacataactt catccaagat ttcattttta tctgggtcca
    49801 caatatgacc atagtttaag gtatgggatt ggatagcgga tagatctttt ccacgataaa
    49861 tttttgatgc tatctttaag gcatctgtac cagatatacg gacaatacca atagctccct
    49921 caccgagagg agttgaaata gctgcgatgg tatcaaattc ttttgtaata gacataaagt
    49981 ctctctcttt cttgggaatt actaacattc attgtaacct aaagccctaa aaggtgcaat
    50041 catttgatta aaagagaaga tttccataat ggttttattt gaaggtatgc tataataaaa
    50101 ctatgattat actacaagga aacaaaattg agcgctcatt ttcaggtgat gtgctttttg
    50161 ataatattaa tattcaagtt gatcaacgtg atcgtattgc tcttgttgga cgtaatggtg
    50221 ctggcaaatc aacgcttctt aaaattttag ttggtgaaga ggctccgaca aaaggagaaa
    50281 taaataaaaa gcgtgatttg agtttgtcct acttggcaca agatagtcgt tttcaatctg
    50341 aaaatacaat tttccaagaa atgttacaag tttttgattc attaagagaa gtagagaagc
    50401 gcttacgtga attggaatta caaatgggac aagtttcagg aagtgacctg gaacaattga
    50461 tgaaaacata cgatattttg tcagaagagt ttcgtgaaaa aggtggcttt acctatgaat
    50521 cagatataaa ggctatttta aatggcttta agtttaacag tgatatgtgg gaaatgccta
    50581 ttagtgaatt gtctggtggg caaaatacac gattagcgct tgcaaaaatg ctacttgaaa
    50641 agccagaatt acttgtttta gatgaaccaa cgaaccattt agacattgat acgattgctt
    50701 ggttagaaaa ttatcttgta aattatcaag gagcccttat tattgtcagc catgaccgct
    50761 attttttaga caaggtagct acagtaactt atgatttaac gacacattct ctagaccgct
    50821 atgtaggcaa ttattcgaaa tttatggatc ttaaggcaga gaaaatagcg actgaagaaa
    50881 agaattttga aaagcaacaa aaagaaattg ctaagttaga agattttgta caacgtaaca
    50941 ttgttagggc ttcaaccact aaacgagcac aagctcgtcg taaacaatta gaaaagatgg
    51001 aacgactcga caaaccgaat gtagagcaaa aatcagctaa tatgactttt catgctggga
    51061 aagtctcagg gaatgttgtt ttaacacttg agaatgcagc tattggttat gaaggtgttt
    51121 ctttatcgga gccaatcgat ttggacgtga agaaatttga tgctattgca attgtaggtc
    51181 caaatggtat cgggaaatca actttaatca aatcgcttgt tgggcaaatt ccttttatca
    51241 aaggagaagc aaaattagga gcaaatgtcg aaactgggta ttacgatcaa agtcaaagta
    51301 atttaacaaa aacgaatact gttttagatg agttatggaa tgctttttca acaactccag
    51361 aagttgaaat tagaaaccgt ttaggagctt ttcttttttc aggtgatgat gttaaaaaat
    51421 cagtttcaat gctatccggt ggtgaacgag cgcgtttgct attggcaaaa ttatctatgg
    51481 aaaataataa tttcctcata ttagatgagc caacaaacca cttggatatt gatagtaaag
    51541 aagtcttaga aaatgcactc atcgaatttg atggcacatt actttttgtt agtcatgatc
    51601 gctattttat taatcgtgtg gcaactaagg ttctcgaaat ttctgataag ggaagtactc
    51661 tctatctagg agattatgat tattacctca ctaagaaagc cgaactagag gaattagccc
    51721 gtttaaatga agaagaagtg agtgcttcaa aaacagaaat cgacgttaca tcagattatg
    51781 agacacaaaa agcaaatcaa aaggaattta ggaaaattac ccgtcgcgtt gcagaaattg
    51841 aggcacgttt ggaagttctt gaaaatgatg aaaacaatat caatggttta atgttagaaa
    51901 ccaatgacat cggaaaactc tctgatttac aaaaagagct ggaatcaatc caagaagaac
    51961 aattgctatt aatggaagaa tgggaaaatc taaatatgag actagatttg tgagaaaatt
    52021 tcaaaaacaa tagtttgtga aaaaaagtac attttaaatt gagatgcctt ttacgaaaac
    52081 aaagaaagcg gttcaaattc ttgcatcttg tgaaaaaaac ttatacaatg attaagtaat
    52141 aaagattttt ttggaggtta gaatggtaac tctatctaaa gaacaacact tggatatgtt
    52201 tctaaaaatg caacgtattc gtgatgttga tatgaaattt aacaaattgg ttcgtcgtgg
    52261 tttcgtccaa gggatgactc acttctctgt tggtgaagaa gctgcttcag taggtgctat
    52321 ccaagattta acagattcag atatcatttt ctcaaaccac cgtggacacg gacaaactat
    52381 tgcaaaaggt attgatattg gtggaatgtt tgctgaactt gctggtaaag caacaggaac
    52441 atcaaaaggt cgtggaggct caatgcactt agcaaacctt gaaaaaggta actatggaac
    52501 aaacggtatc gtcggtggag gatatgcgct tgcggtaggt gctgccctta cacagcaata
    52561 tgaaggaact gataacattg ttatcgcctt ttcgggtgac tcagcgacta acgaaggctc
    52621 attccatgag tcagttaact tagctgctgt ttggaactta ccagttatct tctttatcat
    52681 caacaaccgt tatggtatct caacagacat cacatactca actaaaattc ctcaccttta
    52741 catgcgtgct gatgcgtacg gtattccagg gcattatgtt gaagatggta atgatcttat
    52801 ggctgtttac gagaaaatgc atgaagttat caattacgtg cgttcaggaa atggaccagc
    52861 tattgtagag gttgaatcat accgttggtt tggtcattca actgctgatg ctggggtata
    52921 ccgtactaaa gaagaagttg attcatggaa agctaaagat ccagtaaaac gctaccgtgc
    52981 atacttaatt gaaaatgaaa tcgctactga agaagagctt gcagctattg aagcacaagt
    53041 gattaaagaa gttgaagaag gggttaaatt cgctgaagaa agtccattcc ctgatatgtc
    53101 tgttgcattt gaagacgttt ttgtggacta aaataaccta aagtagatga gatttatctc
    53161 gttctattat tcaattgatt aaaaaacgga tattcggagg aaataatgag cgaaactaaa
    53221 gttatggctt tgcgcgaagc gattaatgtc gctatgagcg aagaaatgcg caaagatgaa
    53281 aaagtatttt taatgggtga agatgtaggt gtatatggag gagacttcgg tacatctgtt
    53341 ggtatgttag aagagtttgg tgctaagcgc gttcgcgaca caccaatctc agaggctgcg
    53401 attgcaggtt cagctatcgg tgctgcacaa acaggtttgc gtcctatcgt tgatttgaca
    53461 ttcatggatt tcgtaacaat cgcaatggat gctatcgtta accaaggtgc taaaacgaat
    53521 tatatgtttg gtggaggtct atcaactcca gttactttcc gtgtggcatc agggtcaggt
    53581 attggttcgg ctgcacaaca ctcacaatca ttagaagctt ggttaacaca cattccaggt
    53641 ctaaaagttg ttgcacctgg tactgttaat gagtcaaaag cacttttgaa atcttctatt
    53701 cttgacaata acccagttat cttcttagag ccaaaagctc tctatggtaa gaaagaagaa
    53761 gttaatatgg atcctgattt ctatattcca cttggtaaag gggatattaa gcgtgaaggt
    53821 actgacttaa caattgtttc ttatggccgt atgcttgagc gtgttatgca agctgctgaa
    53881 gaagttgctg aggaaggtat taacgttgag gttgttgatc cacgtacact tattccgctt
    53941 gataaagaat taattatcga ttctgttaag aaaactggta aattgatctt ggttaacgat
    54001 gcttataaaa ctggtggttt cactggtgaa attgcaacta tggttgctga aagtgaagca
    54061 tttgattacc ttgatcatcc tatcgtgcgt cttgcaagtg aagatgttcc agtaccgtat
    54121 tcacgtgtac ttgaacaagg aatcttacct gatgtagcaa aaattaaaga tgctatctac
    54181 aaagtagtta acaaaggtaa ataattcata aaacaaagag ggactgagga atgagtgaaa
    54241 acttagcctc tttccttgtt tataggtttt aatttatatc gttagatgaa acatatattt
    54301 gacgatatag tttagtgtga aaggaaaact atggctgtcg aaattattat gcctaaactc
    54361 ggggttgaca tgcaagaagg cgaaatcctt gagtggaaaa aacaggtagg tgatgttgtt
    54421 aatgaaggtg acgtcctact tgaaattatg tcagataaaa caaatatgga gattgaagca
    54481 gaagactcag gtgttttact aaaaatcaca catgggaatg gagatgttgt tcctgtaact
    54541 gagacaattg gctgtatcgg tgctgaaggt gaagaagtaa cagaagcatc ttcttctgaa
    54601 aatacatcag ttgaagaaaa cgcgacacaa gtaacttcag aaccagaaaa agttgaagaa
    54661 acatcagaac caagtgtgcc agcagctaca agtggtgaaa aagtacgtgc cactcctgca
    54721 gctcgtaaat tagcaagaga gatgtctatt gaccttgcac tagtatctgg aacaggggca
    54781 aatggtcgcg ttcacagaga agatgttgaa agcttcaagg gtgctcaacc acgtattaca
    54841 ccattggcac gtcgaattgc tgaagatcaa ggagttgata tagcagaaat cactggcagt
    54901 ggtattcgtg gtaaaattgt taaaaatgat gttttggctg ctatgtcacc gcaagcagca
    54961 gaggctcctg taaaaacgaa agctacacca acaacagaag aaaaacaatt accagaaggt
    55021 gttgaagtca ttaagatgtc tgcaatgcgt aaggctattt ctaaaggtat gactaactct
    55081 tacctcactg ctccaagctt tacacttaac tatgatattg atatgactga aatgatggct
    55141 cttcgcaaga aattaattga tccaatcatg gctaaaacag gcttgaaagt cagctttact
    55201 gatttgattg gaatggccgt tgttaagaca ttgatgaaac cagagcatcg atacctaaat
    55261 gcttcattaa ttaacgatgc acaagaaatt gagcttcata attttgttaa tattggaatc
    55321 gcagttggtc ttgatgatgg tttgattgtt cctgttgttc acaatgctga tcaaatgtca
    55381 ctttctgact ttgttatagc atctaaagat gttattaaga aaactcaaga aggtaaatta
    55441 aaatcagctg aaatgtcagg ttcaacattc tcaattacta acttaggtat gtttggtact
    55501 aagacgttta acccaattat caatcaaccg aactcagcaa tccttggagt aggtgctact
    55561 attccgactc caacagtggt tgatggtgaa attgtagctc gaccaatcat ggcaatgtgt
    55621 ttaacaattg accaccgtat tgttgatgga atgaatggtg ctaagtttat ggttgatctt
    55681 aagaacttaa tggaaaaccc atttggttta cttatttgat tccgtaggtc ttaatttgat
    55741 ataataagtt attcgaaaaa cgaaagagga aataaaaaat ggcttttgat gtaattatgc
    55801 caaaacttgg ggttgacatg caagaaggcg aaatccttga gtggaaaaaa aatgaaggtg
    55861 acaccgttaa tgaaggtgat gtccttcttg aaatcatgtc tgataaaacc aatatggaaa
    55921 ttgaagcaga agacactggt gtacttttaa aaattgtaca tcaggcagga gatgttgttc
    55981 ctgtcactga agtgattgcc tatatcggag aagaaggtga agaagtaggt acgtcatcac
    56041 cttcggctga tgcaactatc acagctgaag atggtcaatc agtttctgga cctgctgctc
    56101 cttcacaaga gacagttgca gcagcaactc ctaaagaaga acttgcagct gatgagtacg
    56161 atatcgtagt cgtaggtggt ggccctgcag gttattacgc agcaatccgc ggtgctcaac
    56221 ttggaggaaa aattgccatt gttgagaaaa ctgaatttgg cggtacgtgt ttgaatgttg
    56281 gttgtatccc tacaaaaaca tatcttaaaa atgctgaaat cctagatggt ttgaaagtag
    56341 cagctggacg tggtatcaac cttgcttcaa ctaactatgc aattgatatg gataagactg
    56401 ttgcctttaa aaattctgta gttaagacat tgactggtgg tgtacgtggt ctcttgaaag
    56461 cgaacaaagt tgaaattttc aatggacttg gacaagtaaa ccctgataaa tctgttgtta
    56521 ttggcgataa agttatcaaa ggtcgtaacg tcgtccttgc gactggttct aaggtatctc
    56581 gtattaatat tccaggtatt gaatcaccat tagtattaac atcagatgat attcttgatt
    56641 tacgtgaaat tcctaagtca cttgctgtta tgggtggtgg tgtcgtaggt attgagcttg
    56701 gtcttgtatg ggcttcatat ggtgttgacg taactgttat tgaaatggct gatcgcatca
    56761 ttccagcaat ggataaagaa gtatcgctag aacttcaaaa aatccttgct aagaagggaa
    56821 tgaagattaa aacatcagtt ggtgtttctg aaattgttga agccaataat caattaacgc
    56881 ttaaattaaa caacggcgaa gaagttgttg ctgataaagc tcttctttca attggacgtg
    56941 ttccacaaat gaatggtctt gaaaatcttg agcctgaact tgagatggaa cgtggacgta
    57001 ttaaagttaa tgcttatcaa gaaacttcaa ttccaggtat ctatgcacca ggtgatgtta
    57061 atggaactcg tatgttagct catgctgcat accgtatggg tgaagtagct gctgaaaacg
    57121 cactcggcgg taataagcgt aaagcacatc tagatttcac tccagcagct gtttatactc
    57181 atcctgaagt agcaatggtg gggatgacag aagagcaagc acgtgaacaa tatggtgaca
    57241 tattagttgg taaaaatagc tttacaggca atggtcgtgc tatcgcttca aatgaagcac
    57301 atggttttgt aaaagtaatt gctgaaccta agtataaaga gattcttggt gttcacatta
    57361 ttggtccagc tgcagcggaa ttgattaatg aagcttctac gattatggaa aatgagttga
    57421 ctgtctatga tgtagcacaa tctatccatg gtcacccaac attctctgaa gttatgtacg
    57481 aagctttctt agatgttctc ggtgaagcta ttcacaaccc tccaaaacgc aaataatttt
    57541 gtttaatata tttgagattg agcttttata gcttcaatct cttttcacgt atgtattgtc
    57601 tgaatattat aaaatattag ttaggaattt aaaatgaaat atatcgttaa cacgtctaat
    57661 gatccggctt acaatgttgc tttagaagct tacgcctttc aaaagttgac agatatcgat
    57721 gaaatcttta ttctttggat taatgaaccg gctatcatta ttggacgcca tcaaaataca
    57781 attcaagaaa ttaacaaaga gttcattgat aaaaatggca tccatgttgt ccgtcgtttg
    57841 tcaggaggcg gagcagttta tcatgacttg aataatttaa actacactat tatttctaat
    57901 aatacacaag aaggtgcttt tgatttccaa actttttcaa aaccagttat tgatactctt
    57961 gctaaattag gtgttaaagc agaatttact ggtcgtaatg atttagaaat caatggacaa
    58021 aaatttgcag gaaatgctca agcttattac aaaggtcgta tgatgcatca tggttgtctt
    58081 ttatttgatg ttgatatgtc agtcttaggg caagcactca aagtatctaa agacaaaatt
    58141 gaatctaaag ggataaaatc tgtacgcgca cgtgttacaa atattgttga ccatctttca
    58201 gataaaatta cagtacaaga atttagcgat gccattttag ctcaagtgaa ggaagaatat
    58261 ccagagatgg atgaatatgt tttgagtgat gccgagttat cagaaatcca agctatgcgc
    58321 gataatcaat ttgctacatg ggattggacg tatggtaaag cacctgaata caccattgaa
    58381 cgtggtgtcc gctatccagc tggaaaaatt acgacttatg ctaatgttga aaattcaacg
    58441 ataaaatctg tcaaaatctt tggtgacttc tttggagtga aacctgtaga tgatattgaa
    58501 aaaatgttgg aaggtgtccg ctacgattac aaagatgttt tagcagcgct taaaacagtt
    58561 gatacaagtc aatacttctc acgtatgaca cccgaagaga ttacaaaagc tattgtagac
    58621 taaaatcaaa aaacctagtc aaagtctttg cctttggcta ggttttttgt atggttaagt
    58681 ggc
//
LOCUS       DATRSG010000005        57891 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRSG010000005 DATRSG010000000
VERSION     DATRSG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57891
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.005"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(5..250)
                     /locus_tag="VBD89_000241"
     CDS             complement(5..250)
                     /locus_tag="VBD89_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO7134797.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            complement(350..940)
                     /locus_tag="VBD89_000242"
     CDS             complement(350..940)
                     /locus_tag="VBD89_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO7134798.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(1065..1694)
                     /gene="upp"
                     /locus_tag="VBD89_000243"
     CDS             complement(1065..1694)
                     /gene="upp"
                     /locus_tag="VBD89_000243"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO7134799.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(1804..2973)
                     /locus_tag="VBD89_000244"
     CDS             complement(1804..2973)
                     /locus_tag="VBD89_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7134800.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKGKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(3109..3657)
                     /locus_tag="VBD89_000245"
     CDS             complement(3109..3657)
                     /locus_tag="VBD89_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO7134801.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(3830..5182)
                     /locus_tag="VBD89_000246"
     CDS             complement(3830..5182)
                     /locus_tag="VBD89_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO7134802.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            5388..6737
                     /gene="trkA"
                     /locus_tag="VBD89_000247"
     CDS             5388..6737
                     /gene="trkA"
                     /locus_tag="VBD89_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO7134803.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTNFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            6742..8181
                     /locus_tag="VBD89_000248"
     CDS             6742..8181
                     /locus_tag="VBD89_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7134804.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(8205..8456)
                     /gene="yidD"
                     /locus_tag="VBD89_000249"
     CDS             complement(8205..8456)
                     /gene="yidD"
                     /locus_tag="VBD89_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO7134805.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(8456..9178)
                     /locus_tag="VBD89_000250"
     CDS             complement(8456..9178)
                     /locus_tag="VBD89_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7134806.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(9168..9752)
                     /locus_tag="VBD89_000251"
     CDS             complement(9168..9752)
                     /locus_tag="VBD89_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO7134807.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(9749..10456)
                     /locus_tag="VBD89_000252"
     CDS             complement(9749..10456)
                     /locus_tag="VBD89_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO7134808.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(10456..11196)
                     /gene="xerD"
                     /locus_tag="VBD89_000253"
     CDS             complement(10456..11196)
                     /gene="xerD"
                     /locus_tag="VBD89_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO7134809.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     YQQSLSEFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQTKSQIMDLS
                     NFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPE
                     DLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILK
                     QKENGKTMTELSRLLGLKTPITLERYYR"
     gene            complement(11186..11659)
                     /locus_tag="VBD89_000254"
     CDS             complement(11186..11659)
                     /locus_tag="VBD89_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7134810.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(11656..12177)
                     /locus_tag="VBD89_000255"
     CDS             complement(11656..12177)
                     /locus_tag="VBD89_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7134811.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(12159..13133)
                     /locus_tag="VBD89_000256"
     CDS             complement(12159..13133)
                     /locus_tag="VBD89_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO7134812.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(13130..13924)
                     /gene="racE"
                     /locus_tag="VBD89_000257"
     CDS             complement(13130..13924)
                     /gene="racE"
                     /locus_tag="VBD89_000257"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO7134813.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(14099..14338)
                     /locus_tag="VBD89_000258"
     CDS             complement(14099..14338)
                     /locus_tag="VBD89_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO7134814.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(14496..15038)
                     /locus_tag="VBD89_000259"
     CDS             complement(14496..15038)
                     /locus_tag="VBD89_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134815.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(15100..15621)
                     /locus_tag="VBD89_000260"
     CDS             complement(15100..15621)
                     /locus_tag="VBD89_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO7134816.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(15689..16378)
                     /locus_tag="VBD89_000261"
     CDS             complement(15689..16378)
                     /locus_tag="VBD89_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO7134817.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(16401..16904)
                     /locus_tag="VBD89_000262"
     CDS             complement(16401..16904)
                     /locus_tag="VBD89_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7134818.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(16942..17685)
                     /locus_tag="VBD89_000263"
     CDS             complement(16942..17685)
                     /locus_tag="VBD89_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO7134819.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            17717..17995
                     /locus_tag="VBD89_000264"
     CDS             17717..17995
                     /locus_tag="VBD89_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO7134820.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            18080..19012
                     /gene="yidC"
                     /locus_tag="VBD89_000265"
     CDS             18080..19012
                     /gene="yidC"
                     /locus_tag="VBD89_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7134821.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLILTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(19212..19877)
                     /locus_tag="VBD89_000266"
     CDS             complement(19212..19877)
                     /locus_tag="VBD89_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7134822.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(19901..20758)
                     /locus_tag="VBD89_000267"
     CDS             complement(19901..20758)
                     /locus_tag="VBD89_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7134823.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSNKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(20919..22379)
                     /locus_tag="VBD89_000268"
     CDS             complement(20919..22379)
                     /locus_tag="VBD89_000268"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO7134824.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(22483..22965)
                     /gene="greA"
                     /locus_tag="VBD89_000269"
     CDS             complement(22483..22965)
                     /gene="greA"
                     /locus_tag="VBD89_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO7134825.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(23038..24840)
                     /gene="mltG"
                     /locus_tag="VBD89_000270"
     CDS             complement(23038..24840)
                     /gene="mltG"
                     /locus_tag="VBD89_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO7134826.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKI
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            complement(24927..25430)
                     /locus_tag="VBD89_000271"
     CDS             complement(24927..25430)
                     /locus_tag="VBD89_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7134827.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(25516..26847)
                     /locus_tag="VBD89_000272"
     CDS             complement(25516..26847)
                     /locus_tag="VBD89_000272"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO7134828.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(26857..27474)
                     /locus_tag="VBD89_000273"
     CDS             complement(26857..27474)
                     /locus_tag="VBD89_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134829.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(27639..30737)
                     /locus_tag="VBD89_000274"
     CDS             complement(27639..30737)
                     /locus_tag="VBD89_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7134830.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(30892..32202)
                     /gene="der"
                     /locus_tag="VBD89_000275"
     CDS             complement(30892..32202)
                     /gene="der"
                     /locus_tag="VBD89_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO7134831.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(32250..33152)
                     /gene="dnaI"
                     /locus_tag="VBD89_000276"
     CDS             complement(32250..33152)
                     /gene="dnaI"
                     /locus_tag="VBD89_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO7134832.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(33149..34324)
                     /locus_tag="VBD89_000277"
     CDS             complement(33149..34324)
                     /locus_tag="VBD89_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO7134833.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKLQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(34324..34803)
                     /gene="nrdR"
                     /locus_tag="VBD89_000278"
     CDS             complement(34324..34803)
                     /gene="nrdR"
                     /locus_tag="VBD89_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO7134834.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(34952..36457)
                     /locus_tag="VBD89_000279"
     CDS             complement(34952..36457)
                     /locus_tag="VBD89_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO7134835.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(36447..37136)
                     /locus_tag="VBD89_000280"
     CDS             complement(36447..37136)
                     /locus_tag="VBD89_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7134836.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(37371..37904)
                     /locus_tag="VBD89_000281"
     CDS             complement(37371..37904)
                     /locus_tag="VBD89_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO7134837.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(38009..38899)
                     /gene="htpX"
                     /locus_tag="VBD89_000282"
     CDS             complement(38009..38899)
                     /gene="htpX"
                     /locus_tag="VBD89_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO7134838.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(38920..39474)
                     /locus_tag="VBD89_000283"
     CDS             complement(38920..39474)
                     /locus_tag="VBD89_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO7134839.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            39602..40315
                     /gene="rsmG"
                     /locus_tag="VBD89_000284"
     CDS             39602..40315
                     /gene="rsmG"
                     /locus_tag="VBD89_000284"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO7134840.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            40330..41709
                     /locus_tag="VBD89_000285"
     CDS             40330..41709
                     /locus_tag="VBD89_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7134841.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLSHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            41722..42393
                     /locus_tag="VBD89_000286"
     CDS             41722..42393
                     /locus_tag="VBD89_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO7134842.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINTPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(42428..43258)
                     /locus_tag="VBD89_000287"
     CDS             complement(42428..43258)
                     /locus_tag="VBD89_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7134843.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(43251..44927)
                     /locus_tag="VBD89_000288"
     CDS             complement(43251..44927)
                     /locus_tag="VBD89_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134844.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(44944..45492)
                     /locus_tag="VBD89_000289"
     CDS             complement(44944..45492)
                     /locus_tag="VBD89_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO7134845.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMIKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(45661..46869)
                     /gene="sstT"
                     /locus_tag="VBD89_000290"
     CDS             complement(45661..46869)
                     /gene="sstT"
                     /locus_tag="VBD89_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO7134846.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            47045..48412
                     /gene="brnQ"
                     /locus_tag="VBD89_000291"
     CDS             47045..48412
                     /gene="brnQ"
                     /locus_tag="VBD89_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7134847.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVTYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMTLKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            48526..49581
                     /locus_tag="VBD89_000292"
     CDS             48526..49581
                     /locus_tag="VBD89_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7134848.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(49630..50322)
                     /locus_tag="VBD89_000293"
     CDS             complement(49630..50322)
                     /locus_tag="VBD89_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134849.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(50319..51389)
                     /locus_tag="VBD89_000294"
     CDS             complement(50319..51389)
                     /locus_tag="VBD89_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO7134850.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(51382..52758)
                     /locus_tag="VBD89_000295"
     CDS             complement(51382..52758)
                     /locus_tag="VBD89_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO7134851.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(52892..53716)
                     /locus_tag="VBD89_000296"
     CDS             complement(52892..53716)
                     /locus_tag="VBD89_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7134852.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            complement(53857..54690)
                     /locus_tag="VBD89_000297"
     CDS             complement(53857..54690)
                     /locus_tag="VBD89_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7134853.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            complement(54792..55481)
                     /locus_tag="VBD89_000298"
     CDS             complement(54792..55481)
                     /locus_tag="VBD89_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO7134854.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(55636..56352)
                     /locus_tag="VBD89_000299"
     CDS             complement(55636..56352)
                     /locus_tag="VBD89_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7134855.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(56778..57482)
                     /locus_tag="VBD89_000300"
     CDS             complement(56778..57482)
                     /locus_tag="VBD89_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134856.1"
                     /translation="MRLVWKFLSEKLVCKIMDSSYGVLILFFICLSITAVGGYTLVYN
                     LSGYSSMDAQKVFGIIVLTIILLVFIVGTIFYAIGSAQLIMSILSKKKISFDEKDAPL
                     DGATHVGETVFQGKEVSLYSKQDKFFLWSRKWQKGFSKYQYYHIRPVDQTEISEVYQV
                     ERCCFYCDHIFTQFFIREGKNREKIVELCTIDVQIGNDRDLGFKQVNRGEFEKTVPFK
                     DVEIKEIKKLIDLKQI"
BASE COUNT        18375 a        11386 c         8889 g        19241 t
ORIGIN      
        1 tatcttaacg atataagttt ccaacaaaat cctgatcaat atcatcaatt gctgataacc
       61 gaacatcctt aacaaatttt aaatgtttta tctctttact aacgctaaca atatcctctt
      121 tattaacata aatgactaga tatcttaatc tacgagaatg ataatgaaca tctccgtatt
      181 tatataattt acgagcgtca cgattatagt aaagataaac gctcattcct aaacgctctt
      241 gtttattcaa taattttttc ctttctgcgt catatctgta attactaaga ttataacata
      301 attccaacaa ataaaaaaag agttggtaaa ctcaactctt ttaaatctat tattgtaaat
      361 tattattttc cataatagca tcaataaagc cataatcaag tgtttcttga gcactcatcc
      421 aacgatcacg ctctgcatca tcatggactt tttcaataga ttgaccagaa ttatcagcta
      481 agattttttc taaagtatga cgcgttttta aaagatgctc agcagcgata gccatatcag
      541 attgctgtgt acctccgcct gttccgccca ttggttggtg gatcatatat tctgcattcg
      601 gtaacataaa acgttttcct ttagcaccac ttgaagcaat aatggttccc atcgaagcag
      661 ccatccccat aacaatcgtc tgtacgtccg atttaatgaa gttcatggtg tccacaatag
      721 caagtccagc cgatactgaa ccacctggtg tattgacata aaggtaaata tcctttgtat
      781 tatcttgtgc atcgagaaac aataactgtg caatgatact attggccata ttatcctcaa
      841 cttggcctgt caacataata atacgatctt ttaaaagacg tgagtaaata tcataagaac
      901 gttcaccacg acttgtttgt tcaataacta ctggaatcat atctttctcc atttcttttt
      961 atcactgtca ctattataat catttggtca acaaaggtca aataataact tttttaagaa
     1021 aaggctttct gagttcttca gaaaagcctt attcatctca aatcttattt tgtaccaaat
     1081 aagcgatccc cagcatctcc gagtccagga acaatgtagc catgttcatt gagtttttca
     1141 tccaaagcag cggtataaat atctacatct ggatgtgctt cttgaagtgc tgccactcct
     1201 tctggagctg ctactaaaca aacgaatttg atactagctg cgccacgttt ttttagagaa
     1261 tctactgcta aaatagcaga cccacctgta gcaagcattg gatcaactac aaaaatctga
     1321 cgttgatcaa tatcttcagg taacttaact aaatattcga ctggttggaa agtctcttca
     1381 tcacgataca taccgatatg accaacttta gcagctggca caaggcttaa gaaaccatca
     1441 accataccaa tacctgcacg taaaattgga acaattgcta actttttccc agctaattgt
     1501 ttttgtacag ttgtcgctac tggtgtttgg atttcaacat cttctaaagg caaatcacga
     1561 gacacttcat agcccattaa catcgcaatc tcatcaacta actcacgaaa atctttagta
     1621 gatgttgtag tgcgacgtaa gattgacagt ttatgttgaa taagtggatg tgaaatgact
     1681 tgaaattttc ccattataat gaccgctttc tatttaattt tgagcattaa aaacattcta
     1741 atgcttgttt acattcaaag tattataaca taaaaaccct ttccatgcga ctttttttat
     1801 agtctagtca ttttttctaa agtttgtcac taatcgcaaa actgcctcct caataacaga
     1861 cctaggagct gcgacattga ttctagcatg ttttttccct tcttttccaa aagttaaacc
     1921 atcatttaat attaatttgg catcatatcg aagtttttct tgaatttcta aatgcgttaa
     1981 attataagca gaaaaatcta accaaacaag ataagttccc tctggtttca tcactttgat
     2041 atgagttttt tgcgttaatt gctcataaag gtattctatt gatccttcta actccatttt
     2101 tagggccttt aaccattgtt tcccttttgt aaaggcaact tctgtagcta ataaccccaa
     2161 tgaagaaatt tcttgttgat tgttagctat ttgcctcttc ttaaaatccg agcgtaactt
     2221 ttcattttca attattgcaa aactattttt agtccctgca atattaaacg ttttagttgc
     2281 actagataaa ataacactca attctttaaa actagaatca acagtattaa agctatggtg
     2341 tacattatca aataatacca aatcttgatg tatttcgtca gaaacgagga taacattata
     2401 tcgtttacag atatctccta ttttttgaat ttctcctttt gtccagacac gcccaccggg
     2461 attgtgtgga ctacaaaaaa tataaagctt tacattattt tcaatgatat ctttttctaa
     2521 ttgtttaaag tcaatctcaa aatactgatt attattaagt aaactatttg aaactaaatg
     2581 acgattattg tattttatag ttctagcaaa gggcggatag actggggtat taattagaac
     2641 agcatctccc tcctttgtga aggcttgtat agcaactgaa atcgctggta caaccccatc
     2701 aattaataat aatgattttt tatcaaattg ataaccatgt tcttgcactt cccaattaga
     2761 gatagcttgg tataaactgt ctttaggact atcatagcca aaaaccattt ggtgtgaaaa
     2821 atcttcaata gcctccgaca tttctggaaa gataggaaaa tccatatccg caatccaaag
     2881 gggaaggatt tcttgatctt tttgaaccgc cttccatttt atggcattac ttgaaaaacg
     2941 ttctggtaaa cttgtgaaat catatgtaat cataattact cctgactatt ctacagctaa
     3001 aaatatccct tgtcgccttg gtgactattt taccatttgt ttttgatact atatactaat
     3061 taacattcta aagataaaag tgaccatttt aaacgatcac tttctttatt attttaattt
     3121 atcattttca taagtatggc ttaattctaa attaggaaag ctttgctgcc taagggcttc
     3181 ataaacaatc atacaaacag tgtttgaaac attcaagcta cgcacatgct catcgttcat
     3241 gggaattcgt aaggcctttt cggcatgctc gcgcataaaa gtttctggca atcctttatc
     3301 ttcacgacca aacataaaat aatggtcttg atcatcattg taattttcat ctgagtaaac
     3361 cttgtcagca aatttgctaa ttaggtgaac tttccctcga catgacaaca taaattcttc
     3421 caaaccatca tagaaactaa catctagctt atcccagtaa tccagtcccg ctcgtttcat
     3481 ttttttatca tcaataggaa atcccatcgg tcgtatgatg tgcaaaggag catttgttgc
     3541 cgcacaagtt ctagcaatat taccagtatt tgctggaatc tgtggttcaa ataaaacaat
     3601 atgatttcgt ccgctatcgg aacgatgatt tttctgcgtt agtgtttcaa tattcatctc
     3661 atcatcactt tctagaactc attctgactc gcaaaaaaac cacactgctc ggagtcaatc
     3721 tcagcaaaca atgtggttta tggtggtttg ttaacttaaa tcataacagg tttgatttaa
     3781 actaacaaat cgttgacact attatagcac ttcagtctac aaagtcaacc tattcatgag
     3841 tttttgggtt atttttttga tagatataat gacaaatcaa acagaataat aagcaaaaac
     3901 attggaaaat taaaaccgta aagaaattac ttaacaaact cccgataagg aggatcaaac
     3961 cgcctaaaat tgctagaatc ggtgaaacta agccggtaaa tttattttta atatcttttt
     4021 tgagataaag gctgagtact aaaacataaa gaatgattaa actggtatta ttaaacacca
     4081 cagcaatttc actaatatca ctacccggca ataagttaaa ttttgtaacc ataaaatgaa
     4141 caaaaagcca aaaaatagct acagccgtca ccgtcaaact agctggcaat gacatttgat
     4201 acttcaaatt tatgttagcc atcctctcac ttttgatcca tccgcgttcc gcaaaagctt
     4261 gaggtagacg catcgtccca agtaaaagtc cattggcaac acctaataca gatagaataa
     4321 caatgaaaga taataaacgc ccaacactag gaccgaaaat aatattagct gcataattaa
     4381 tagcatcatt accggttgtc ataataaagc tagcacctaa aatttgtgtc aaaccataga
     4441 aaaatgccaa atatgataat aagataagcg ctggaccaat gacaaaggca agtggtaaat
     4501 tcttcttggg attcttgact tctggtgcaa tattaacaaa aattgtccaa ccatcatatg
     4561 caaaatacaa tggtaccaga cctgacaccc aactccatcc aacatttgac ggttgaatag
     4621 ctgttaaatg ttgtggaagt tgtggagctt tatcagacca aaaagcacca attaaagcaa
     4681 ttagaactaa aggaatcatt tttacaaagg tcgttaaaga ttggaatccc cctgcaatac
     4741 gcttagcaaa gatattcatg agactaagcg ctaaaatata aacagcagct aaaataatct
     4801 gtgattccaa acttgatgat tcacctaaag tataaaatgc agctacccat gaaacgattg
     4861 ctgttaaagt tggaagatac aaaaaagacg caaacaagcc taaagttgct gctaaagctg
     4921 ggctaacata tttttcgtag taagaaaaaa taccaccact ttcactcgtt ctaatagcta
     4981 gctcagaaat tgacaaactt ccaaaaacaa tcgaaaaaga acctagtact aaaattacca
     5041 tgcctaaaaa gacatctcca ccagtaaatt taaggatgtc atcaacttta aaataaatgc
     5101 ctgaaccaat cactacacct acaatcatag caattgtagt cattaatcca taggtctgtt
     5161 taatagaatt atgattctcc ataatatctc catttctact gtacgcttaa aggttaatta
     5221 taacctaatt tagggcttgt cacaagttta tctgtgttat ttttcaccag attctgataa
     5281 gagaatcata ttactcttat cagaaagatg aaaacgtttt ttaaccataa ttctcgtgca
     5341 tttttactaa aaaaaaggta tcatagagtt tattattgga ggtaaaaatg agaataattg
     5401 ttgtcggcgg aggtaaagtc ggaaccgcac tttgtcgctc acttgttgct gaaaagcacg
     5461 atgttgtttt aattgagaaa aaagaaaacg tccttaaacg tgtcactaaa cagcatgata
     5521 ttatgggaat tgttgggaat ggagcaaact ataaaatttt agaacaagcc gaagtcaaaa
     5581 attgtgatat ttttatcgct atcactgatc gagatgaagt taatatgatt tcagcagttc
     5641 ttgccaaaaa aatgggggca aaagagactg ttgttcgcat gcgtaatcct gagtattcta
     5701 atccttactt caaagataaa aactttcttg gcttctcttc agttgtcaac cctgaattgt
     5761 tagcagcaca atatatcgct aataccatag aatttccaaa tgcaacttca gttgaacact
     5821 tcgccaacgg gcgtgtgatg ttaatggaat ttaaaatttt agaaggaaat aaactctgtc
     5881 atacttcaat gagtcaaatt cgtaaaaaat ttggtaatat tgttatttgc gcaattgaac
     5941 gcgatggcaa attgattatt cctgatggcg atgcaactat ccaagtcaaa gataaaatct
     6001 ttgtaactgg aaatcgtatt gaaatgatac ttttccataa ttacgttaaa aataaagttg
     6061 tcaaaaactt gatggttatc ggtgcaggac gcatcgccta ctacctactc aatatcctta
     6121 aaaataccaa tacacacgtc aaactagttg aactcaacca agagcaagct gaatatttta
     6181 gtcaagaatt tcccaatgtt cctgtcgttc atggggatgg tactgctaaa aatattttgt
     6241 tagaagaaag cgttacaaat tttgacgcag ttgctacttt gactggtgtt gatgaagaaa
     6301 atatcattac ctcaatgttc ttagaaagta tcggtatccc taaaaacatt accaaagtta
     6361 atcgaactag cctactagaa atcattgacg ataaacaact atcaagcatt atcacaccca
     6421 agcgtattgc cgtagaccat gtgatgcact ttgtacgcgg gcgtgtaaat gctcaggatt
     6481 ccaacctaga agctatgcat catattgcta acgatcgcat tgaaacctta caatttgaaa
     6541 taaaagaaac tagcaaatta gcaaatcgta gccttgcatc tttaaaatta aagcaaaata
     6601 ttttaattgc agcgattatt cgcaataata aaacgatttt cccaactggt gaggatgttt
     6661 taacagtggg tgatcgtatc gttgttatca ccttattaaa aaatattact agaactagtg
     6721 atatgttagc gaggtaggaa tatgaataaa agtatgattc gtttccttct ctcaaaactt
     6781 ttgctaattg aagctgctct tctagcgatt cctctaactg ttggacttat ttaccgtgaa
     6841 ccgcaaagtg tcatgatgag tatagtcatt accatgatta ttttaatcat ccttggatta
     6901 ctgggttctc tctttaaacc taaaaattat catatctaca ctaaagaagg catgttaatt
     6961 gttgcactct gctggatact ttggtccttc tttggcgccc ttccatttgt tatttcaggg
     7021 caaatcccta atattatcga tgctttcttt gaagtatctt ctggttttac gacaacagga
     7081 gcaactattc ttgatgacgt cagtgtctta tcacccgcgc tcttattttg gcgtagcttt
     7141 acccatctta tcggtggaat gggggtttta gtatttgccc ttgccatcat ggaaaattct
     7201 aaaaatagcc atttagaggt tatgagagct gaagttcctg gacctgtatt tggtaaagtt
     7261 gtctcgaaac ttaaaaaaac tgcacaaatt ttgtatttgt tatatcttct tatgtttgca
     7321 gtatttgcag taatattata ttttgcaggt atgcctttct ttgatagtat tatcatagcg
     7381 atgggaactg caggtacagg tggttttgca gtttacaatg atagcattgc tcattataat
     7441 agtcctctga taactaactt agtttctatt ggtatgctta tcttcggggt taactttaat
     7501 ctttactatc ttcttttact ccgaaaaatt aaagcattct ttggtgatga ggaactaaaa
     7561 acctatctcc gcatcgtggc aatagcaact ttcatgattg ctctaaacgt catcggtatg
     7621 tatgataatt ttcgacaagg cctcgaacat attttctttg aagtatcagc tattattacg
     7681 acaactggtt ttggcgtgac tgatatcact cgttggcctt tgttttcaca ggttattttg
     7741 ctattcttga tgtttatcgg tggttctgct ggctctacag ctgggggctt taaagtcatg
     7801 cgtagcttaa tcctagcaaa gattgcacgt aaccaagtat tgtctacact ctatcctaat
     7861 agagtcatgt cactacacat taataaaagc gttctagata aaaatacaca acatggtgtt
     7921 ttaaaatatc taactatcta tttagctatc ttcatggcac tcgttcttgt tttaacgctt
     7981 gatactaatg attttttggt tgttatcagt gctgcagcct catgttttaa caacataggc
     8041 ccactcttag gtagcaatga aactttctca ttctttagtc cattctcaaa actccttctt
     8101 tcatttgcta tgatagcagg acgtttagaa atataccctg ttttattgat gtttattcct
     8161 aaaacatggt caaaactata attacaaata ataaaacgga tatatcaatc tgatatatcc
     8221 gttttattac gtcttaagct aaaatgatca gggacaggat catttcctcc gtgggctaag
     8281 ggatgacatc gcaaaatacg tgcaatcccc atcaacacac cttttagacc atgtttttga
     8341 atagcttcta tcatatacgt agagcaagtt ggacgataac gacagctagc tgggaaagct
     8401 ggagaaatat ttttttggta aaagcgaact aagaaaatca aaaaagattt aagcattaag
     8461 atttattaat agccgcatta tgcaactgac taatttcttt tttatttaat cgtcgtgctt
     8521 ccccaggacg gagacccgtt aaatctaagg taccgaattg tgtacgagat agcttgtcca
     8581 ctaataaccc aacttgttca aacattttct taacttgatg attacgacct tcatgaatag
     8641 ttaattcaac tactgaacga tttttttcat gatccacttt tatgatagta tagcgagcag
     8701 gttttgtttt cttaccatcg ataacaacgc cacgcgttaa tggtcgaagg ttttctttag
     8761 tagctattcc tttaacacgt gctaaataaa ctttatcaat ttcattacga ggatgaatca
     8821 ttttatcagt aaaatcacca tcattagtca aaattaaaag tccagttgta tcccaatcca
     8881 agcgaccaac aggatatatc cgttctttga cttgtggcaa caagtcaata actgtcttac
     8941 gtcctttatc atctgagaca cttgaaataa caccacgtgg tttatttaag agataatata
     9001 ccttctcttc gttgtaaatt ggacttcctt caatttcaac cagatcacca gcctttactt
     9061 gggtcgccaa ttcattaacc acttgaccat taatcgtgac cattccttgc ttgattaatt
     9121 cctcagcttt tctacgactt gcaatccctg catgcgcaat atatttatta attctcattg
     9181 ttttccttat cttctgtatt aattgaatcc attgaaaata acgatacttc ttcgtcaact
     9241 aattcaattg aagaggcgtc aattaagtca tctaactgat taattcccat ataatctaag
     9301 aaataattag ttgtctcata aaggttagga cgtcccaaca cttctttctt tccagcctct
     9361 ttaattaaac caaaagctat cagtctcgtt atagctccac tggaattcac gccacgaata
     9421 tcatcaattt caatcctagt aataggttgt ttataagcaa taatagatag aacttccaaa
     9481 cttgcccgag atagactctg attaatcgga gccttcgcat aatctctcaa caaagtcata
     9541 taactatcct ttgttaccaa tttataagtt tgtgctgtct caagcaataa tagactagaa
     9601 tcgtcatctt cttcatatcg tttagccaac ttttctagtt gctgtattaa tgctgatggt
     9661 gtcaaagaca ataattctgc catttgacgc aaactcagtc catcttcacc tgcaacaaaa
     9721 agaagagctt ctattgagcc taaataagtc atttctcttc ctttcttaag ataacttgac
     9781 taaaattact gtcttgttct actgtgattt gatgtaattt tatcaactct aacattgcca
     9841 aaaataaagt tatcatttca gattttgttt ggcaatccgc aaacacttct tgtaatgtcg
     9901 ttttcttttt aagattaaaa tgtctttcaa taacgatcat catatcttca atacggtagt
     9961 cttctttctc aattaccgtt tgactatttg acaattcttt ttgtttttgg gacatcattt
    10021 gagaaaaagt caaaaaaaga tccatcaccg atttatcgtg tagaagaatt gcatcttcaa
    10081 agataacttc ttgttttggt tttgaaaaat atttggcgcg ttcctgatgc tgattagcta
    10141 actcttcact tagcacttta aaacgtctat attcctctaa ttggctcaac aattccattt
    10201 ctggatcatc ttcaatagga tttgactcca ctactttagg taataagtta cggcttttta
    10261 tcaacatcag ctgacttgcc ataagcatat actcaccagc tacttctaga cgcatcgcct
    10321 gtaatgttgc tatataagca aggtactgct caataacttc cacaatcggt acatcgtata
    10381 tatcaacctc atatttagat acgaggtgca agagtaagtc taggggcccc tcgaaatctt
    10441 ttaacttaat atccattatc tataatatct ctctaaagtg ataggagttt ttaagcctag
    10501 aaggcgactg agctccgtca tcgttttgcc attttctttt tgctttagaa taaattgttc
    10561 tcgcagaagt tgtgcagtta gttgttgctc attcttctca tttaagaaat ctgttaattg
    10621 attaaaaaac cattgtctag aataaggctt accgttatgc tcaaagacta agtcaccatc
    10681 ttcctctaaa gattccaaaa gaaaaggaag caagtcttct ggaagtttta aaatacgctt
    10741 catctgcgat ttttcaatac ttaagatatt aaaagttgta tcaaaatcag ccttcttcaa
    10801 attagcaatt tcagccggag ttaaacctaa agataaaatc aacaaagcaa tcaaacgacc
    10861 actcggataa tcagtatctt gatagaaatt tgataaatcc attatttgag actttgtctg
    10921 agccaaagtg attttttcag tctcttgtag tctgtaaaat tccttcagcg ttcctctatt
    10981 atataaaaag aaaagaaatt gattgactgc agataacttt cgcttacgag ctgttaactt
    11041 aaattcactt agagactgtt gatacaatgc cagtttttca gaatttacat gtccaccaat
    11101 tattttataa aattgcttta aatcatagtg ataggactta cgcgaattga gagataattt
    11161 cttactctca ataaaattat taatatcatt tatcattgtt tttaggagtg atagtatatt
    11221 cgtctgtaaa atcatgtagg aggctattaa tagcttttaa aatcgattta cgggttataa
    11281 tgcctctaaa atcattttga tcactgataa caggtaaaaa tggataatct acaatcttat
    11341 gcataattgc tgtcagagta gctgcctcat taatcggctc gatttttgta ttaaccatct
    11401 caacaatatc agtctgtgcc atctcccaat ctgttaattg acctttagat tggtagccca
    11461 taatgtctga gatactaata gtacctacat attttttctc tttagtgata actggtacac
    11521 gtgaaaaacc attacttgct aatagtaaca taacgtggtc tgcattgtgt gtatccacaa
    11581 aaatagcaac gtcttcagct ggaattagat agtggtccaa atgatttagc aaaaacgact
    11641 caaattcttt agcaatcatc ttgaaaattc ctttgtcaag ttaggatagg gttggtgttg
    11701 gcgagtataa tactctacca aaaccttact atctgttata gttacaacag cgtaaagctt
    11761 ctcatttatt ggtcctcttg gctgcaaaac actacctgga ttgataaaaa tagttttacc
    11821 atttttccaa gcatctgcac gatgaagatg accatataaa caaatatccg cgtcttcttg
    11881 ctgagctaat aaatccaatt tatcccaggt aaaattaatc tgatggagat gaccatgggt
    11941 ttgaacaata acagcattat ctatttttgt aaccaaaact tccggataac ctgaatcata
    12001 atcacaattt cctgtgacca catgaatgcc ttcccatata ggatctgagc taggaagttc
    12061 ggaatcccca ttgtgaaaaa tagcatccac ttttcccaaa taatgattct ttatatcttt
    12121 tacaatgtca cgatctccgt gagaatcact cattacaact aattttctaa ttgccatttt
    12181 ggaaatacct ccatcaattt acggacagct tgcccacgat gtgatagatt atttttttct
    12241 tgtgcgctca attcagctgc tgtcctacta ccttctccga caataaagag cggatcataa
    12301 ccaaagccat tttccccctt aggttccgta ccaatataac ctggccactc tgcttcaact
    12361 accaaacttt ctttatttgg agctgacacg actaaggttg tatgaaactg tgctgaacgt
    12421 ctttctttgt caaataccat ggctaattca tgcaataatt tggcattatt acgagcatct
    12481 gtcgcgtcag gacctgaaaa acgagcagac catacccctg gcaaaccacc taaagcatct
    12541 acttttaatc cggaatcatc tgaaattacc atttttccag ttaattttga aatagtctct
    12601 gctttaagtc gagcattttc ttcaaaggtc ataccagtct cttcaacttc tggtaaatct
    12661 gggtaatcat ttaaattttc aactttgagt cctaatttcc caaataattc tctaaattcc
    12721 ttcgtttttc cttcattgtg agtcgcaatt aatatggtat ccccaaaatt attatcattc
    12781 tttaaatcaa agaatttttc taaagaagtg ccctctttag gtaagtgaac taagaacaac
    12841 tgatcatctt cactaactaa gatggcccct ttatgttgag tcactttgat atgccttgaa
    12901 tactcttgat taatcatatc aacgaggaat gctaataatt gaaaatccga agtcatctgc
    12961 acaacgtgaa gctgtagacc ttcaactttc aaagttgata atagacgatg aaaaacatct
    13021 tggatagctt cgtaagattc atcattacca aaacaagtaa aataattaaa agcttggaaa
    13081 ctacctaaaa accaattgcc ttcagtttta ctttcaaaaa ttgtttttgt cataatacga
    13141 tacgctccac atttatttct tgactaagcc attgttctgc aatttcttta aaaccttttg
    13201 ggctggcggt tgtgtaaaag tgatgaccac cgtgtttatt ttgccaatta tggtttatct
    13261 caaaatagtt caataaaaca gaaatatcac gaacggtttc tgcgccacta tcaattaatt
    13321 taacctcagc ccccataaca ttttgaatga tgggacgtaa taggggataa tgcgtgcaac
    13381 ctaaaattaa agtatctaat ttaccaacta atggggacaa cgtttcataa accacctttt
    13441 tggctaaact agaagacatc tgatttgatt ccacaattgg aacaaatttc ggacaagcaa
    13501 gggataccac agcagtattt ggagacaaag cttgaatttt ttgacgataa gcatctgatt
    13561 taacagtcat gggagtacct ataataccaa ctttccctaa attagttgat ttgatagctg
    13621 cgctagctcc tggtaaaata acgcctaaaa cagggatgtc tagtttttct ttaatttctt
    13681 gccaggcaac tgcagttgct gtattacaag ctataacaat catcttaaca tttttagtca
    13741 ataagaagtt aaccatctgc caggtaaact ctctaatctg ttgagcaggt ctaggaccat
    13801 acggagctct agcctgatct ccaatgaaga ttacttcctc ttctggaagt tgacggaaca
    13861 tttccttaac aaccgttaaa ccacctacac cagaatctag aaagccaata ggcctactat
    13921 ccatttttat tcctccaata ttagtgacta aaagaggctg gacaacagtc ctagcctctc
    13981 tatacaataa ccaatcagtc aaatctacac ttgaccaagt ttgcccaaat cttgtctaga
    14041 aatctgatgg actatcactc actatttcat cactaaacgc ttcaatgcta aaacactctt
    14101 attttttagt tttgatagct gtttttgcgt gttttacaat attacggtat gtttgttgaa
    14161 ctttagcttc actaggtttt tgtcccattt gactcatcat ttcacgaata gcatccggtg
    14221 ttaaacgagg gtgctctcca atctcttttt caatttgttt acgagcaata aaaatgcctc
    14281 ccacgagacc accaaacaaa gcgacgataa ttaataaaat ccaaatagtt gttgacatct
    14341 attcattctc ctcatttatt tacatgactc attatagcaa aaaaagaaag agtttacaaa
    14401 tatatgtaaa caaaatcaaa gcctctatcg tccttgtcac gtcaatacat tattaaattt
    14461 tcactagagc atggttaagc acatagtcga gtcctttatc ttatcctaag tgttttcccc
    14521 aacttttgtg tcatccattt ccaaatgaag agagctatgt aaaaatctac caatccatgt
    14581 agcaaagagc ctagtcctat caaagaaaat agtgaccata aataagttga actactgtta
    14641 gaagtcattg tgattatata aacagtaata aattcgagca gaccatgaat tatgttaata
    14701 gctacagcaa ataacaatgt ttgccattta ctcatcaata aagagggctt tgacttaatc
    14761 aagaaagccg caattaatgc aaatccgata tgtgataaag ctctaagaac gattacaatc
    14821 ggaaaaccag ctagtagaaa tccaagtcct gtccctaaag ctaccaatat tgcaactgga
    14881 acagatataa aaatacttaa aaataaaggt acatggcttg ctaaggtgaa agaagcaggc
    14941 ccaataatta ttttagcagg catgattata ggaatcatga ttgcaaaagc tactaaaata
    15001 gaagtataag aaaccatttg tgttgttttt tttgacattt ttatctcctt tgaagctatt
    15061 atacaaaata accttacagg tgtaaagaca cctgtaaggt tatttgtcta tgtataaaat
    15121 tcctgctttt tttagagcct cacatatttg gtcaaaaact gactgatctt gacaagaaat
    15181 catatgtgta tgtagaccat ctgtcaaaga ggatagcaat tctgcattgg aaacttttaa
    15241 tttagcagta aaattatcaa tgtctttttg gctttgaatg tttaagggag cagtcagcat
    15301 cccataaata gggtgctcta cctccacatt cataatgatt ccttgatacc gcaaaataat
    15361 ttccagttct tcttctgtct gttctatgcc atgctgacaa accaatctag ctgtgaattg
    15421 atgagtactt agagcactgg acataaggta tccttttggg gtagaaataa tatcacattg
    15481 ttgtgcacgc aataaggcaa tatctccaac aatcacctgc ctactaacac taaaaatctt
    15541 tgctaatgtg cttgccgata tagcttcttt tgttcctttt aaagtcgtta aaatattttc
    15601 tcgacgctcc tgtgccttca tataacaaat tccttctttg gtgttatttc acaattaaaa
    15661 actggctaga atatctaacc agttagcctc aatcatttcg tgcaaaaaga cgaagaatat
    15721 ttaagaatag attaataaaa tcaagataaa ggcttagtgc catcgaaact gcccaaccat
    15781 ctgcgacttg gccgccttgg ctattgtaga catatttaat catttggtta tcgtaagcaa
    15841 tcaatccaga aaagatgatg acacatacga tactaatgat ataactcata cccccactac
    15901 cgataaataa attaacaaga cttgcaatta aaatcccaat taaagcagcc attaacgctt
    15961 tacgcatacc tgataaatct tttttagtct tagctccaat caaagccatt gcaaagaaaa
    16021 cagccgcaga tgttataaaa gcttgaagaa ctgtagtttg cgtgtacctt gcgataatga
    16081 aacttaaagt aaacccattc aaagctgaat aaatcaaaaa taatggtaat gcagctggtg
    16141 tgtttcgacg cgccgctcca ctagcaacaa agactaaaac caattctaag atgattgctc
    16201 caaaatacaa ccctggcatt ttttgcatga tggcaatcat attttgtgga aacatataca
    16261 gcataatcgc agaaacagca gctgacaaac ctactcctat ccctacaaga ccgtaaatct
    16321 tagcaaaaaa ttgattcagc cctgaatcag attgtgtgta aatgacgtta tcattcatat
    16381 gtattttcct attctaaatt ttaatgatta tgactaccta agaatttgcg actaaaacgg
    16441 ataggtttct taaaaatacg tttgagtgga cgagaagcct ctcgtactgc ccaatactta
    16501 tcaaccatag tcacaatata actttcttta taacgtggcg gtgcaatagt cgctccccac
    16561 atgtgcttca aaataatatc ttcttctcta gcgtttaaat cagttaattt tcgcgcatta
    16621 cgaaccgcta tccttggatg aacccaggca tggctcttgt taaatttcgt tacacgccaa
    16681 tcataataaa agaaatcatg tagtaaacca cctcgagcag tactactcgc atcccaacca
    16741 aacttcttag caattttata acttgtataa ctaacattta tagaatgctc taagcgagtt
    16801 gagtgatgat gttggacaat agcttcgagt ttttggaaac gtggatggtc aataaggtga
    16861 ccgaccaaat ccatgaagtc cttatcgtct tgatactttt tcattataat cacccctctg
    16921 gggatattat aacatatgaa cttaaattaa gctgaaaatg actactcccg cagctactgc
    16981 tacattaaga ctttctgctt gtccaggcat ttcaatatgt gctaaaacat ctgcttcttc
    17041 tgtcatagtt ttggaaattc cctgtccctc atttcccatg attaaagcaa aataatcata
    17101 tttagggaga ttcttataat ttaccgattg ttcagataaa gttgtcgcaa tcatagttat
    17161 cccttctgct ttacaaaagt taacaaactg ctctatttct accctaaata ctggtaaatg
    17221 aaagtgactg ccttgcatag aacgaagtgt tttttggtta tacaaatcag cagatttctg
    17281 agataaaaaa acagcatcaa aattcgctgc atctgcagtt cgaaccattg ttcctaggtt
    17341 tcctggatcc tgcacatctt ctaatacaag aaaacgtcct tttttaatat ctgtccatct
    17401 tgtctcttga aaggcaatct cagctacaat cccttgagga gtctgtgagt cagttaattc
    17461 tttcaaaaca tcatctgtaa caacaatagc tctctcgggt tttctcaaac gatctattgc
    17521 cgtttctgtc acaaaaatat ttaaaaattg tgcaccatat ttttcagctt cctcaaataa
    17581 atgccagcct tcaataagat atgaagattt ccgatatttt ttttgtaaca actttttcgt
    17641 ttttttaatg agattatttg attttgaagt tataatagta gtcataaacc tattatatca
    17701 gaaaagaggt caagttatga aaaaagttca tttaattgtc tcagggcgcg ttcaaggagt
    17761 cggatttcgc tatgcaactt attccttagc tttagaaata ggtgatattt acggtcgcgt
    17821 ttggaataac gatgatggaa ctgttgaaat tttagcacaa tccacagatt caaataaaat
    17881 gacacaattt attcagaaaa ttcgaaaagg accttcaaaa tggtctaaag taacttatgt
    17941 tgatattaaa ctagataact tcgatgattt taatgacttt aaaatgagta attaaaaatc
    18001 attctgcgga agtattgttt aattcttaaa aaaaaagtaa aatagatagg tatattaaaa
    18061 attagaggaa ctaaaaacat tgaaaaaaac attgaaacgt atcttattct ctagcttatc
    18121 tttgtctatg ttattattat tgacaggatg tgttagcgtt gataaggctg gaaaacccta
    18181 tggagttatc tggaatactc ttggagtgcc tatggccaac ctcattactt atttcgctca
    18241 acaccaaggg cttggttttg gtgtcgctat cattattgta accgtcatcg tacgtgtcgt
    18301 tatcttacca cttggacttt atcagtcttg gaaagctagc tatcaagctg aaaaaatggc
    18361 gtactttaag cctttatttg aacctattaa tgaacgacta cgcaatgcta aaacacaaga
    18421 agaaaaatta gcagctcaga cagaattaat gacagctcaa cgtgagaatg gccttagcat
    18481 gtttggcggt attggctgct tgccattact cattcagatg ccatttttct cagctatttt
    18541 ctttgctgca cgctatactc ctggagtctc tagtgcaacc ttcttgggac ttaacttagg
    18601 tcaaaagagt ctgatactta ctgtcattat cgccattctt tattttgtac aatcttggtt
    18661 gtcaatgcaa ggtgttccag atgaacaacg tcagcaaatg aaaacaatga tgtatctgat
    18721 gccaataatg atggtattca tgtctattag tttacctgct agtgttgcgc tttattggtt
    18781 tattggtggt attttcagta ttatccagca gctagttacg acttatgtac ttaagcctaa
    18841 actacgccga aaagtagaag aagaatatac taaaaatcct cctaaagcct acaaagctaa
    18901 taacgcacga aaagatgtta caaattcaac taaagccact gaatctaatc aagcaattat
    18961 tacatctaaa aaaacaaatc gtaacgccgg aaaacaaaaa agacggggat aaaacaagca
    19021 tactaaataa tggtgtgttg tcctaaacag ttaaagatta ttatgtaatt caagtaaaaa
    19081 agacttagtt ctatgtccta catagctaag tctttttagt tttgcctaac ttttcatata
    19141 aaaagcaccc taaaagtcag ataaaaatct agcttttaaa gtgcaagtaa tctagctaac
    19201 agactacctt attaataaag taatcgtttt tcccaccagt cttgaataat tgcataaagc
    19261 gtacaaatta cccaatagat aagggctact aagatatagg cggtcatata gtcggagtag
    19321 gttcccccaa ctattttagc attattaaag atatcaggta ccgtaatcat cgcagtcaat
    19381 gaggaaccct tgaccatatc tagcaaaaca ttacttaaag aagggagtgc aattcttgtt
    19441 gcttgcggaa taataatgcc tctataaatc gtcggtgttt ttaaccctaa agctctagca
    19501 gcttcccact gaccatgatc aacagctaaa agagctgctc tgataatttc tgatatatat
    19561 gcactagaca tcatggtaaa acctacgata gaagcagtca cagcatctaa ctgtatccct
    19621 ataaaaggca aaccaaagta gataagaaag agtagaacca ttaaaggaat cccacgcatt
    19681 aaggaaatat gaatattagc tagatacctc agtaagcgga aatgagacat cttcattaga
    19741 gctacaaaga atcctacgat agctcctaaa ataaagccaa ccaaggataa tccaatggta
    19801 tatggtaacc cctgaataac acttggaaaa gccttaacag ctagttccaa attaaaaatg
    19861 gcatcccagt ttatcactta tctactcctc tcgaaatttt ttatttaact ttaattactg
    19921 gaattttatc cgttccagca ggttcttttg tgagatcttg accagcataa tatttttcag
    19981 agattttttt caaagaaccg tctttcttca tatccttgat agctgcatca atttttgttt
    20041 tgaggctctt atctttttta ctcatcacaa taccttgctc agtcggatta tacttcacat
    20101 cacccatttt tactttgata tctggatatt gttttgttac atattttaca gcaatgactt
    20161 gtgtgtagta atcattcgga atgaaatctg tacgtccagt tgatacatct cgaaggtaaa
    20221 cgtcattagt tacattgtca taaataacag gttcagcacc ttgctgtttt gcaattttca
    20281 tatattgcgt tccagcgcca ccaccagctt ttttaccttt ccagtcagat aaatcttttg
    20341 ctgtaatctt tgaagaacca tctgcacgta caatcatacc tccaacagaa tatttataag
    20401 gttgagagaa attgtatttt ttcaaacgct cgggagttgt gtcaaagtta ttgacagcaa
    20461 catccacctt accactatct acagaagtaa atgattcagc aactccaact tcaacaaact
    20521 taacttttat cttaagtttt ttagcaatcg ctttcatcat atcaatctca taaccagtca
    20581 attttttatg gtcatcataa taacttgttg gataaagcgt tgcaggggta gcaaccttaa
    20641 ggacaccatc ttttttaata ttgtcccatg ttgtttcttt gttagattga ccatttgagc
    20701 aagctgctaa tgtcattgca gataaaccga ctaaaccaag taaaattgtc tttttcataa
    20761 gtactcctgt agattaaatt atattaaaat ggtttggcgt actctatcga aaattaaatt
    20821 taatgaaaat taatacataa atttacgata ataatacaac cattgttaag agtaccatat
    20881 tgtatccgtt ttcgcaaaaa tattgtttac ttttcaaatt aatggtaaac aggccctttg
    20941 aattgcttct ctttttcaaa caactctgca acagcaagca ataaatcttc tctcccttta
    21001 gccgctgtca attgtacacc taaaggtagc ccctctttag ttagatgagt tggtatggct
    21061 aaagaaggtt gccctgtcaa gtttggctga tgtgtaaaag gtgtccaggc cattgagtct
    21121 tcaaacattt tccaaattaa atcctgttgt tcacttactg agaattctcc catatgcctc
    21181 aattgtttct ggagtgtctc gtctaaatca aattgtccat gaaaaggagc tggttgatta
    21241 gttgctgctg ttaaaattaa atcataattt tcatgaaacc tcgccatggt agctgcaaat
    21301 tgatcccatg aatctaagag tttactgtaa tctttagcca ataccctttg tccactctga
    21361 tacatagccc aggtcattaa ttccatatcg ctaaattcca ttgaacgccc taaactcttt
    21421 tcgatgtcat caaacattgc tgctgtttca acgctattca tcatacaata agttttcata
    21481 gaatgaatac catctaaagg ttgttctact aattctaaat gattcccttt ttgagacaga
    21541 aaatctgcgg cttctttaat agctgcttta gcctcacttg atacctttgt ttttaaggga
    21601 gaatccatca atactgctat ttttaggggt ttactaacgc taaactcgaa aagactctct
    21661 ttgctgagtt ttttcagggg aaatgggctt tctacttgat atgattgtaa ataatataat
    21721 agtcgttttg tatctctaac tgactttgtt aaagcaaaat gactagaagc cccttgccaa
    21781 cctctatagg atgaaggtcc aactggtata cgtcctcgac tcggttttaa accaatcaaa
    21841 ccattaaaac ttgctggaat tctgattgac cctcctccat cactagctcc tgcaattgga
    21901 accattcctg aactaacagc tgcggctgca ccaccactag agcctccagc atttcttgaa
    21961 tggtcaaatg gaagattaac attcccgtga agttgaccat cactaatatt cttaaaacca
    22021 aattcagggg tatttgttct tcctaaaata ataaacccta atttttcaaa ggactgcact
    22081 aaatagtccg tttgtttagc atgatagtgt ttaaataatt gtgaacctga ggttgagagt
    22141 tgtccttttt gattctgtcc caaatctttc agaagaatag gaactcctgc aaaaggagca
    22201 tttgattcat ttgcgtattt ggctgcttga cgcgcctcat tatattgctt gctgacaaca
    22261 gcattaacgc ttacattctg ttcctcgatc ttgtagattg cttgctctac taattcttgc
    22321 gacgaaatct tatgctgttt tatcgcttgt accatagcgg tagcatcttt aaaaaccata
    22381 taaacacctc ataaaatgta tttttattat aagataacaa aaaatactta aaaaaaatag
    22441 aataacgtct agactctaga tattattcta tgatttatac tattaatttg ttttttcaac
    22501 actaatgatt tcaacttgat agctaccagc aggtgattca attgtagcta agtctcctgt
    22561 ttttttacca attaaagcat gagcaattgg gctttcattc gaaatttttc ctgaaaaaat
    22621 gtcagctcca gcagcaccaa cgatatggta agtatcttta tcatttgtac caacttcttg
    22681 gacaaggaca gttttaccaa ttgcaacctc atctttagca actgcatcac tatcaataat
    22741 ttcagcataa cgaatttttg tttctaaaat ttgaatttga ccctcaacaa atgcttgttc
    22801 gtcttttgct gcatcatatt cgctattttc tgataagtca ccataagaac gagcaatctt
    22861 gatacgctct acaacttctg gtctacgaac taattttaac tcttcaagtt ctttttcaag
    22921 ttgatctttt tccacttgtg tcattgggta agttttttct gccattttat ttctcttttc
    22981 ttttcatttt ttcaagtcaa agatataaac atgatgactc gcaccatgtt tataagctta
    23041 gttaatttga ctattgatat atttttcaac gttagccttg tgtgtttcaa aatctttagc
    23101 gtagtataca tttccggtct tcacatcagc gacaaaataa aggtaatctg ttgaagcagg
    23161 ttttatagtc gcttcaattg ctgagatact tggactatca actggtccag gcatcaaacc
    23221 agtattagta tagatgttgt atggtgattt aattgaagta ttaatttgag catcttcagc
    23281 taaacttgtt ttatcaccta atttccccat agcatacaaa attgcaatgt tactttgaag
    23341 agcttgaccg gctgataatc ggttataaaa gacacttgct attttacggc gatcttcgtc
    23401 tgttgatccc tctttttcaa caagagatga taatgtcaaa acatcattta cagacatatt
    23461 cttcgcttta atagtgttat agtaaggtgc tatcttagta ttcattgttg aaatcatatc
    23521 ttcaacaagt ccttctaagg ttgtatcttt ataatagttg taggtagctg ggaataaata
    23581 tccttccaat tgataaatag cttttgattt atcaggtaaa cttcctagta aatttggata
    23641 tttagctacc atttttttaa taaacgtttc atcttgaaca agtttcaaga aatcatcagc
    23701 tttataaggt gtacttgtag tctttgtatc aattttatta gactcaatag ctttagcaat
    23761 ctgtttaata gtgtaaccct cggtaattaa tatcttacct aaagctggtt ttgtcggttc
    23821 tgcagttccg cctttttcca attcagcagc aatctgatct aaagtcatac tttttttaag
    23881 attgtagtat ccactttgaa aattactata gtttttgaac ttggaatagt aattaaagac
    23941 tgtggcgctc ttaatgactc ctgctttttc taaaatttga ccaattaatt tattcccaga
    24001 tcctatcgga atttcaactt gtacaaattt atttgaattg gaatcaacag gtttaatagc
    24061 actatctaca aaacgataac ctacaaatgc tgtcaagagc aatacaagta tgataattga
    24121 tatcagaata cgtgcaattc gttttgccac attatctgct ttctgacgtc tttcacgctt
    24181 gttatgtttg ttcttggctg acaaagctat accctcttct tcttttgaca aatcctctac
    24241 tcggataggt tcctttgcta cgggctctaa ctctgatgtc tcaccaagtt ttacattttc
    24301 accttctgta ggaatatctg ttgctctcac aagaaagatt tcttccgttt cttgattagg
    24361 cacagcagtt gcaacatcag tcttatctgc ttttaaatct atgtcaggta cattttcagt
    24421 tgtcctaaat tcactagatt tggagccgct atcaagctct cttgaaggtt ccatcccttg
    24481 attgataact tgttcttctt gggaagctaa tgcttgttcc gcctgacgtc tttcttcttc
    24541 tcgagcagct tctgccagaa gtttcgcctc aatccgagcc ttctcttgct cttctaagcg
    24601 ttgcttagtc tctagcgcct ttgctctagc ttcttgttcg tctttcaaac gttgcgcttc
    24661 ataatctgcc atcaattgtg ctgttctgcg agcagcttct ttggcttctt gctctttttg
    24721 atagagttct tcttcacgca actttctcaa acggttagct tcttctaact ctgccagaat
    24781 ttgttcttta aaacttttct gatcatggtt agaatgctga tcgtcattaa attcggtcaa
    24841 agagatttcc tcctaataat aatcacaggc aattatatct taaatagtct ataaaatcaa
    24901 caaaactttg acaatgttat ctacttttaa caaattgatc gtgttggatt attccaaatc
    24961 atattatacc aaagcgaacc accatgttgg gagtcagatt ctccctcatc tttaaatcca
    25021 ttcatttcat agaaggaaat taaatcatca tggcaagtta aacttatacc atctcgttct
    25081 tgagaaacaa ccaggtcttt catcgctgct aataatgctg taccaatccc ctgaccttta
    25141 aaatcaggat gtattgatag actagtaatt cctatgaaac cacctaccct tacagggaat
    25201 tcagaaactt tatggaataa atcgtccgtt aagtaccgac ctttaataac cggtccttca
    25261 atgtaacctg ctaaacgacc attaatctct gcaactaaaa aagtatcagt cattatcgtt
    25321 aaacgttctt gcattgcttt tttactcgca gcctctgctt ctgaaaaatt tatttgttca
    25381 atactaatga cttgctgtaa atcctctagg caaccatttc taataatcat actcatctcc
    25441 ttaatttctt tatgaaattc tcttgataaa gtcagataga ggactgagaa acttctctca
    25501 gcccttacca tcaacttatt gtgtattttt agttaggtta gctaataatt cttcaaaaga
    25561 gcgctcatac aattgaatgt ctccagcacc cataaagaca tagacagcat tatcatgatt
    25621 gagtaaaggc gagacatttt cgactgtcac taaatctgag tgtttgacaa tcttagcagc
    25681 taaatcttct accttcacct caccattatc tacttctcta gcagaaccat atatttgagc
    25741 gagataaacg ctatccgctt gactcaaggc atgggcaaat tcgtctaaaa gagctatcgt
    25801 acgagtgaac gtatgcggtt ggaaaatagc tacaatttct tttgacgggt atttttgtcg
    25861 agcagcatct aatgtcgcaa taatctcagt aggatggtga gcaaagtcat caataatgac
    25921 agtatcgtca ataatcttct cagtaaaacg acgctttacc cctgaaaatg tcttcaaatg
    25981 ctcagctact aatgccatat caattcccat tatgtaaagg ttagcaataa cagcagttgc
    26041 atttaagata ttatgtttac cgtatgctgg tacatgaaac tgaccaattt cttcttggtt
    26101 atagaaaacc ttaaagtcag aaccattaac agttcgagtg atgtcttttg ctataaaatc
    26161 atttgaatct tcaaaaccat aataatatat tggtgcctca gaagtgattt catgaagttt
    26221 tggatcttct ccataaatga ataaaccttt ttgaacttgc ttagcatagt cattaaaggc
    26281 attgaatacg tcctctaggc ctgtaaaata atcaggatgg tcaaaatcaa tattggtaat
    26341 aattgagtat tctggatggt acggcataaa atgacgttcg tattcatcag cttcaaacac
    26401 aaagtaatta gcattagcag aaccacgtcc tgtaccatct ccaattagga aagaagtgtc
    26461 tgtaatattt tttaaaacat gagctaataa acctgtcgtt gaggtttttc catgtgcccc
    26521 agctacacct agactagtga actgacgcat aaaatctccg agaaattcat gatatcgttt
    26581 aaaatgatag cccttttcaa taacataagc caactcttca ttgttatctg gacgaaaagc
    26641 atttcctgca ataatctcta aatcctcact gatattattc ggtgagaaag gtaatatagt
    26701 tacacctgct tgctctaaac cacgttgggt aaaataatat ttgtcaacgt cacttccttg
    26761 gacgttatgt cccatttgat gaagcatcag tgctagggca ctcattccgg atcctttaat
    26821 accaataaaa tgataagttt ttgacatggt ctctccttaa ttaatgtctt taaaacgtgt
    26881 cagatttaat tcttgagcct tggctctttc tttatgaaac tgattgtctt gtttattgta
    26941 aacttgactg cgtttcaaaa aatcaaaatt atttttcttt gtttttgggt tcggtgtatt
    27001 atcaggttct tgaaatatga ttggaatttc tgctaatata tagctttcct gctgtaaacg
    27061 atcagaaaga tgacttagtt cagtcacctt tcgctgagtt tgttttttcg gtactgttgc
    27121 cttcaattgt tgctgaaaag cttcctttga tggcgcctta aattcctgtt tcgcatagta
    27181 agcttgtcgt ttctcccgaa cagacctctt tgcctcttgt cgagatttgc tcaactctgt
    27241 cgcttttttt acaaaactct gcggtggtac aggatcattt tcaattacct taggatgtga
    27301 tatttcttca tgttcataat tgcttttgac catgctttga tatgtttttt cttgataaaa
    27361 gcctcgaatg ttgttaatta aatcttcatt atcataaaga ttcatcttta caagcggaga
    27421 cattactggt tcatcatctg ctatcaacgg aaatcgtcta gagcctctat tcacattatc
    27481 acttcctttc tacaacagac ctattataaa ctaatttttc tgttttttca aagttttcaa
    27541 ctaagtaaag cttatcagat tggtattttt cattcattta tataaaaaag aaagcgagag
    27601 accactttct tttttatatt atagtcacat catcatattt atttagaaac tccaagtatc
    27661 tctcgaatat catctacgct catgctagca tgggtttcat ttccatccag aactgttgta
    27721 accaaatgtt ttttagtttc ttgcatttct agaatttttt cttcgattgt gcctctagta
    27781 atcaagcgat aaacctcgac attctctttt tgccccaacc tatgtgcgcg actaatcgcc
    27841 tgcatttcaa cagctggatt ccaccacaag tcaatcagaa caacggtatc agctcctgta
    27901 aggttgagac caacgccacc agcttttaaa gaaatcaaga aagcatcttt tgatcctgca
    27961 ttaaatgctc tggtcatttc atgacgttca tttgctggtg tagaaccagt aattttataa
    28021 gttgtaagcc ccatagctac catctcacgt tctgcgatat ctaacatacc cctgaactga
    28081 gaaaaaatca gagcacgatg tccattttcc ttaatttgtg ttaaaagttg acgtaggctt
    28141 tctaacttac cgctttcacc atcataatcc ataaaaagac gtggagtgtc acaaatttga
    28201 cgtaatcgtg tgatgccaga taaaatttca attttacgac gatttaagtc agcgtcacta
    28261 gaatgttgga tactttcttg aatttggcgt aattgcgcca aatagataac tttttgactg
    28321 tctgtcattt catttggata gttcatttca atcaaatccg gtaattctgg gagaacttct
    28381 tcttttcgac gtctcatcac aaatggtttg atataacgag caacttgttt agggttcaac
    28441 ttcaaaaact ctttcttacc aggtaataaa ccaggtaaaa tgatctggaa aatggaccaa
    28501 atttctaaca gtttattctc aataggtgtt cccgataatg caaaacagtt tttgacctca
    28561 aaagatctca agctatgggc tattttagtt tgagcatttt tcataacctg tgcttcatcc
    28621 aaaattaaat aatcataatt tccttcagaa taagtctcaa aatcctgcct aaatgatgaa
    28681 taacttgtaa tgattacttg atgacgttca gcaataattt catctctacg aatcttagaa
    28741 ccataggcaa ctgctacatc tacgtctggc gcaaatttgt ggaattcatc ttgccaatta
    28801 taaattaaac ttgatggtga taaaataagc acacgagaat ctctagttag ttttgtactt
    28861 aaaaatgaaa tcgtttgaag tgtttttcca agccccatat catcagctaa aatccctgca
    28921 aagccataat gatctaacat acttaaccaa cggacacctg taagctgata atcgcgcaag
    28981 gaagctgaaa cagatagacc aggtatttta aacttttctg ggtgtctcaa atcttcaatc
    29041 aattggtaaa aatgttgtga aaagctaaca ttatcttgat ttgcaaacag ttctgataat
    29101 tgaaatgctg caatattatc taattcaata tgcccattct ttgctcgtct agctctcaac
    29161 ccttgcaaag ttgcacttac tttcttagtt tcttcatcaa aaataactaa ttgccctgat
    29221 ttattcacaa aataaggatt attgtcaaac aaagctacca gagcttgatc aacatcgttt
    29281 tccagtaaat cagagaaatc aaaagaaata tctagcaggc ttcccttagc ttcaatatca
    29341 atttgcggtc gttctatgag acgataactt tccaattttg cggataagct cactgttccc
    29401 atttttttaa attgtggaag catataggta taaaaatcgt ataattcttg tgcactgtat
    29461 gctggatggc tggttgaaaa gtggggtgca aagccatatt tttctaagag tttaaatact
    29521 ttttcttcat gtttgaagtg actagcaaat gttaactgtt ctagttcttg acgattatga
    29581 acagttaaat cattaccata atcaaatacc atttgcaata aaatttcatt ttgactatta
    29641 atatcgaact caaaatttac cttaaaatca tgaatagaga agctccgtgg agcatccact
    29701 agtccaatct ccttaaaatc cagtaaatga gctgctaact tatcttgatc atctaaatca
    29761 aaatggatat gttttgctaa atctccctca atgggaagac tacgaatagc cgttaccatc
    29821 ttttcttgct tcaaattaag atggtagaaa gtatcgcgat aaaacaaata agaattcgca
    29881 aatagtggtt ttaagtcttt ctctttgatt tctaattcaa ttgattgcct atggacaata
    29941 accttaaatt gataaagatg atcctcacct tctaattcct taaagtagag gtgattaaat
    30001 tgatgaaaat cactttcaaa tgaaaaattt tctaaagcat tcatcaaagt aacaccctct
    30061 tcaaaaaaac cacgagtgag actaaggtga cgaccttgat ttggtaaaat aaactctaga
    30121 tcaattttgc ttgaatgact tggaatgagc ctccataaaa attctatcaa ttcttgactg
    30181 gtctcatcaa actgtatcaa agacaaagtt tcaaagtatt gttttccaat ctgataataa
    30241 gcttcttttc gaacagtatt taaaaaggct ttgatgtcac gaataacata acttctatca
    30301 tcaggcagtc gtctaatttt taaagtccac cagatatcat tagcataagg attttgttct
    30361 ccttgagccg ataattgata ttttatagta tcatcttcat taataattag actatctaaa
    30421 aaaacactac caaaagaggt tttcttctta gtttcttgtt gactttcttg ttgaacttga
    30481 actttagata agattgcttt accttctgga tcgtttttca agaaatgctc cagagcagct
    30541 aaatgttgac aatagccttt tcttgcaaaa aaatcacaag aacattttgt ttcttctgtt
    30601 actaatgaat attcaatctg acaatcccca actttagctt ttaatagatt accttcttga
    30661 gaaatcaagg acaccaaacc ttgttcgtag agttctatac cttgatttcg aatacgtcct
    30721 ggaatcattc tactcattat gctaccacct tcatcttatc ccttaattat aacaaaattc
    30781 aagtccaatt tcttgtaaaa atactaaatt gattgttttt tgatacagta acaaataaaa
    30841 aaggctaaaa caactgtcct aaccttctat ttcaatttca acaataagat gttatttacg
    30901 tttacgtgct attagattaa ttggtgttcc ttcaaaaaca aatgcttcac ggatttgatt
    30961 ttctaagaaa cgaagatatg aaaagtgcat caattcttct tcgttaacaa aaacaacgaa
    31021 agttggtggt ttaacagcaa cttgagttgc gtagaaaatc ttaagtcgtt ttcctttatc
    31081 agttggtgtt ggattaatag caatagcatc cataatcaca tcatttaata cagcagacgg
    31141 aatacgtttg ttttgacttt cgctaatacg tttaatcata tctggtaact tgtgcaaacg
    31201 ttgctttgtc tctgcagaaa caaagataat tggcgcataa gacaagaatt ggaagttatc
    31261 acgtatatca gcttcccact gactgacagt atgattgtct ttttcgatag tatcccattt
    31321 attaactaca ataataatac ctttaccagt ttcatgtgca aaccctgcaa tacgcttgtc
    31381 atactcacga ataccttcct ctgcattaat caccataaga acaacatctg agcggtcgat
    31441 ggcgcgcata gaacgcataa cagaatattt ctctgtgttt tcatatacct taccagattt
    31501 acgcatacca gcagtatcaa tcatagtata ctcttgcccc tggctatcaa cgaaattagt
    31561 atcaatagca tcgcgcgtag taccagcaac tgggctagca ataacacggt cttccccaag
    31621 gatggcatta attaaactag atttcccaac atttggacgt ccaatcaaac taaaacgaat
    31681 aatatctgga ttttcatttt cctcttctac cggaagattt tccacaatag catccaatat
    31741 atcaccagta ccaataccat gaactgatga caagggataa ggatccccta aaccaagaga
    31801 gtagaaatcg taaatatcat tgcgcatctc aggattatct accttattaa cagctaaaat
    31861 aaccggttta ttggtcttat aaagaatacg tgagacatac tcatctgcat cagtcacacc
    31921 ttctttaccc gacacaacaa acacaataac atcagcctct gtcatagcaa tgtctgcttg
    31981 gtgcttaatt tgctccataa acggtgcatc aacatcatca atcccaccag tatcaattaa
    32041 tgaaaactta cgatttagcc actcacccgt tgtatagata cgatcacgag tgactccttc
    32101 aacgtcctca acaatggaaa tacgttctcc tgcaattctg ttaaataatg ttgattttcc
    32161 aacattcgga cgaccaacaa tagcaactgt aggtaatacc ataattctcc tctcactatt
    32221 gggaatactc ccaattccat tctattttat taacggcgat tcggtccttc taagtggaat
    32281 tctatcgcta aataccgtac gcgctccata actcgttttg cttgccaagt ttcgtcgctt
    32341 ccttttatat tagcccattt tctttccaaa tcgttaaaac tataatttga cgtaaagaaa
    32401 gtgggcaact cttctagcat acgatgttgt aatataactt gtaaaatctc gtcacgaacc
    32461 caggacgttg cctgttcagc cccaatatca tctaaaatca aaatcggaac acttttcaca
    32521 gcatcaattt catcttttac agttccgctt gaaatagcat ttttgacatc aatagcaaaa
    32581 ctagggaaat gaagaagagt cgttgacaca ccctttctct ctgataattc cctcgccatt
    32641 gctgccatta aataggattt cccaaccccc atatctccat ataaataaag ccccttgtga
    32701 ttatatgaag gataagtctc tacaaaatca agcagttgtg acatagcttt cattcgactc
    32761 tcgttattaa tatcaaagtc agtcattctg atattccgat aggacttggg tagattaact
    32821 aaattaatac gatcactgat agcttgtttt ttttgtgctt caattaactc tcgtgtttct
    32881 aaataagaaa catctgcata accttcattc ataaccaaaa taggctcata gcctttagca
    32941 atatattgac tatccttatt tttaaatttc tgacgctcaa tcaaaaattg attaaattta
    33001 gacatgctga tatttatttc acgctgagaa agttggtggg ttttaatgaa ctccgctacc
    33061 tgagcatctg ctaaaatcat ttgaataagc tcatcatttg tattacgagg cactctccct
    33121 tgattttcta atgcttgtcc cactgatttc atgattcacc atcctttcct aatcgttcta
    33181 aacgcttgag ggcatctgtt ttaaactgtt ctaattcaat ttctttttct gggctaacct
    33241 catctttata atcaggatta ctccattcag gaacattcga ttgtttcttc ttcgtttctg
    33301 ttttagtcct tactttttga tctgaaaaat cacgaatttt aagcacagca tcttcggccg
    33361 tcataacatt ctgaaaggca aaatcattag caactttcat gatataggcc ttatttaagt
    33421 tagcagattt tgtcttattc aaggtatata aaaccataac attaatgact tcgtctagga
    33481 aattcatttt tgccaaatcc tcaagtaaag tcttctcacc acttgttgtc actgccttac
    33541 ggcttctctt gattttttca aggaacacta gagcactatc atttttagat tcccttagta
    33601 tgaccttttc attatttgta aaactctgat tatctttaat atgctgcccc tcattttgtt
    33661 gaagtttcat tcgctgaggc gcaatcattc cattgatagc tgtttgtttt gctaattgat
    33721 aggtatcata ccaatttaaa tgataaagtt ctgatacact ataaatacct aagacatcat
    33781 ctttttcatt gttaaaacgt aagccatctc gcatcatcaa acgtttgaaa ctttccaagt
    33841 caaaaacatt tttactccgg tttacctctt gttttggttg tcctaaatcc gagaaaacat
    33901 ctgtaaattt tttagagata tctctagcat ttttaggtat tttcatatct aattctgcca
    33961 ctgctacctc tcctatacgt ttttctaaca aacgacgata gatgggatta gataaaaata
    34021 actcatttga catagcttga ttcaacttaa caaggtaagt acctgtggct tggtaaacag
    34081 tcaacaaatc caaagctgtt aacattacca atgcatcttg aaatcttggc atcccgtatt
    34141 gcagatgatt gagaacctct gaaaatttat gacgtcgtat tccgtcatca aaaaaatgaa
    34201 caaaataatc atagagtgcc atggcatcac ttcccataat tgggaaatac aattgtatta
    34261 aactattact atcataaaca atcttatttt gcttaatata tgtaaactca tctattggac
    34321 tcatcacacg ctaccactct ttttactccg tacgcgcttt gtaatttgtt gaagtagttc
    34381 ttcaatttca tctacatctt taaaagattt ataaacacta gcaaagcgca cataagtaat
    34441 ctcatctaat tcggcgagtt catccatcac taaatttccg atagtgatac tagaaacctc
    34501 atcttcatac tcactcctaa ttttacgttc gatacgtaag atacaatttt caatatcttc
    34561 gctcgaaaca gggcgttttt gagcactctg aataatacca ttcaaaattt tatctcgaga
    34621 aaattgctcg cgagtaccat ctttctttat gactaaaaga ggcaattctt ccaaacgttc
    34681 aaatgttgta aagcggttgc cacatttttc gcactctctc cggcgtctga tagtcgtacc
    34741 ttcttcagct tgtcggctat caaccacact agatttatta tagccacatt ttggacaacg
    34801 catgtcaaca cccctattct atcatatcat ttaatcatct tattttaaca caaattcagt
    34861 aataacaaaa gaagcgcctc tcttatcacc tcatcatatc aaaacacaaa ataatcctta
    34921 tgccttttat cattaagcat aaggattttt cttatatttc tttagtttct tcaaattttt
    34981 ttataggaat gcgaacaatg aattcagtcc cttgatttag ttcactttta atatccacct
    35041 ttaaatggta agcatccata atttgtttaa acactgaaag cccgatacct aaaccagctt
    35101 gtgtactttc tctgttacgt gatttatcag ttcgataaaa acgatcaaag ataaactcaa
    35161 tatcttcatc tgagatacct tctcctttat cttttacaac tactgtcgca aaatcattgt
    35221 ctactgataa aacaacactg acttctttat aactaggaga gtacttgata gcgttatcaa
    35281 ttaagatcat gagagcttgc tcaaaatgta tcttatatat ctttccccaa acgatatctg
    35341 aaatattatt ttcaaaaata aattgaaaat cttctctcaa aatacggaaa ttttcaataa
    35401 caactgatat agagcttgat aaatctgtca cttcatcttg atgaagttcc aagcttcctt
    35461 ggacgcgaat catatttaac atgtcattaa tcattaagga catacgatct gcctcatggt
    35521 atgccgctgc taaactttct tcaagaattt ctggatcatc tttcccccaa cgttgtaata
    35581 aaccaatatg accctttaca acagcgacag gcgttcttaa ttcatgacta acatcactaa
    35641 tgaattgtga ttgtcgacga gtatagtctt ccaactgatc caacatccca tcaaaaatcc
    35701 tagacaattc tccaatttcg tcttctgaac ggaccttact tctaatttct aaattactag
    35761 gattttcaga tatctgatgc attaagtcgt gtaaattttt aaccggacga acaatacgct
    35821 tagtagcact attaataacg accaaggcta aaacagtccc taaaacttcc atcataatca
    35881 agaaaacaat gatgttttgc ctcatactat aataacgacc taaattatag aaaatttgaa
    35941 cgtatccaag caactgtcct gttttttgag agtagattgc ttttgtacca acaaaacctt
    36001 ccatatctgc aactttagag atgataactt ttcccatgga tttaccaata gttggcatac
    36061 ccgttctcag tgtagaaaaa actggtttcc tgttgacatc gtatagggtc atttcttgat
    36121 taacataaag aatactatcg attgactcgc ccgttctaac aattccattt tgacgcttat
    36181 gaggatagat gtcatccgca aaaacctggt tgtacaggat ctctaaaata tcatcggatg
    36241 tcaaactaga gttcgcttgc gacaaacgaa ctctagtttt ttctaatgac ctaccgacat
    36301 taattttttc cctgtgaagg acgtatcttg ttgccgaagt ataaactaaa acagaaaata
    36361 ctgtaaatag gctaaaaaat aagataaaaa ctaattgcga taattttttt gacagaggtt
    36421 gttttacccc tataaactga tcttttttat ttttcacgaa tcacatagcc cattccgcga
    36481 acagtttgaa tatatgattc acggcctggg atatcaattt tacctcttag gtaacggata
    36541 taaacatcaa caacgtttgt ctctgctgcc acatcgtatt tccaaacatg ttctagcaac
    36601 tcttctcgtg tcataacacg attcatattt gtcatcaaga cattcaacaa atcaaattca
    36661 cgttttgtta gtgaaatttc ctcatcacca cgcatcgctg aacgattatg agtatttaat
    36721 gacaaatcac ggaaactacc actatcacct ttttcttttg tcttcgtttc aatttcttgg
    36781 cgtcggaaaa tcgctctaac acgtgctaat aattcttcga ttgcaaacgg cttaacaata
    36841 taatcatctg ctccacgatc aagaccagct acaatatcca taacagaatc acgtgctgtc
    36901 atcatcatga tataggttgt tttttcagcc tgcaggcgac gtgtgatttc gaaaccatcc
    36961 atctctggaa gcattaaatc cagtagaatc aaatcaaaat ctttttctaa tgctgtgtcc
    37021 aatccttcac gaccgtttgt ttcaacaaca acatcatatc cttcatgtag tagttctaac
    37081 gagacgaagc gagctaaatt tttctcatct tcgattatta agatcttttt acccatctac
    37141 accaaccttc ttattcttta cttaacacta tcaattattt tttagaatat ctagataaaa
    37201 aacaagatag cccacatttt actaacgttt ctttaaataa ataaatcgcc atcccaaaca
    37261 attgttcgat agcgagactt aatttttatt attgagtagt gtcctctgtc acttttcaat
    37321 tctactaaaa aaaaattaaa atatcaactg atgttattaa taatttttat ctaatcacta
    37381 tcaaacatac cttctaaggc tgcgaaagga ttagattttt ctttttcttt agcttgtttt
    37441 tcttcatact gcttttcagt cattaacgac caatttttac ctgataaatc cgcttctacc
    37501 ccaacctcat cagctgccag aacacgaaga ggtatattta acaagatatt atcgataacg
    37561 ctttcttcta aattaatatc atcttcctct aatattaaga ccaactcctg atcaacgagc
    37621 tcttttttag tgctaacatt ttcagcttca ataaaaactt cattaatagt ttgcttttca
    37681 gataaaacaa ctggtttcat agaacgacta gacggtaatg taatatcgta agccatatta
    37741 tagtcaagta gatagagacc atcttcatac accacatgac ctgaaacttg aatatcagaa
    37801 atatccatta tctctgaatg acgctccata agcgactctt tgatatctat ttttttatca
    37861 aaataaagcc cctctggtga ttttttgatt tctgtaagta acacgtgcca tctcccttta
    37921 tagtatatgt catattatat caagttttac aggctttgtc ttaaaaaaac aaaacgcaag
    37981 gcatagctac cctacgtttt gttaacgact acatatgacg caaacgctca atacgatctg
    38041 caattggtgg atgcgtataa aataaagact ttaacccttc ttttttcgtt gggtcattga
    38101 tatagagagc cgcactagca tcatctactg gatgccccat aggttcagaa cgatcaagtt
    38161 tttctaaagc agagatcatt ccttgtggat ttcttgttaa ctcaacagaa ctagcgtctg
    38221 ctaagtattc gcgctgtctt gaaatagcta attgaactaa actagcggct aatggtgcaa
    38281 ggattagtga taaaatagag aaaatcaaaa ccaagatgtt tccaccatct tctctgtcat
    38341 catcacgcct acgacctccg ccgtaaaata acatacggct accaatgctt gatattagag
    38401 ttacagcact cgctaaagct actgcaatgg ttgaaatacg gatatcataa ttacgaatat
    38461 gactaacctc atgtccaata acaccctcta gctcttcgcg gttcataaca gctaataggc
    38521 ctgtcgttgc tgcaacagca gcattttctg gcttagagcc tgtggcaaag gcatttaggg
    38581 agtcatcttc aacaatgaaa acgcgtggca taggtatctg agctatcatt gccatatctt
    38641 caacaatatg aaaataattt ggcgcctcat cttctgtaac ttcacgcgca ttgttcattg
    38701 acataacaac attagttgat tgaaaaatca tacttactgc atagataacc ccaataatta
    38761 gagctagaac aagcccaaat tggtagctgc ctaataccaa ataacctaca gctgctccaa
    38821 tagctgctaa aagacagaag aagacaatta acaagacaac tgtttttctc ttattacttg
    38881 caatttgttg atacagcaaa agcctctctc ccttctcaat taaaagtcaa aggaaacttt
    38941 tggcacttct ttttcttctt caggagtttc taagaattgg cttggtttga acccaaatag
    39001 ttttccaaca atattacttg ggaaagtttc taatttcaca ttataattgg atgttgttgt
    39061 gttatataac tgacgtgaat aagaaatttt attttccgta ttagttagtt cttcttgaag
    39121 tttaacaaag ctattgttgg cttttaaatc tgggtaattt tcagcaaccg ctaaaatact
    39181 tgaaagctgt cttgttagct cattacttgc tgtcattgct tcagcaggtg tgttagcttt
    39241 agcaacttga gcacgcagtt ctgcaatctt ctcaagtgtt ttcccttcgt acgcagcata
    39301 gcctttaaca gtttcgatta ggtttggaat taaatcgtta cgacgtttca attgaacgtc
    39361 tatttgactc catgattctt tagtatgcat gcgacttcta actaaactgt tataagcaac
    39421 aatcaaccaa ataacaaaca aagcaataat tgctattaaa atcatagttc ccatagtagt
    39481 agctccttta cattttttcc attatagcaa ttttctttag attaggaaag tcctgttatt
    39541 cccattatta gtatttgtga taaaatagag tcattatacg atatagaaat tgaggtttac
    39601 tatgacaccc caagcatttt atcaagttct gatagagcat ggtattacac ttactgacaa
    39661 acagaaaaaa caatttgaaa cttattttag acttcttgtt gaatggaatg agaaaattaa
    39721 cctaacagct attacagata aggaagaggt ttacttaaag cacttctatg attctattgc
    39781 gccgatttta caaggttata ttgacaatag tcctcttagc attttagata ttggtgctgg
    39841 tgcaggtttt cctagtattc ctatgaaaat cctttatcct gaaattgata ttactattat
    39901 tgattcactg aataaacgca ttaattttct aaacatatta gctaatgaac ttgaactttc
    39961 aggagttcac ttcttccatg ggcgcgctga ggactttggt caagataaag tatttcgagc
    40021 taaatttgat atcgtaacag ctcgtgcagt agctagaatg caggttctag cagaattgac
    40081 aatacctttt cttaaagtta atggtcgctt gattgcactg aaagctgcag ctgctgaaga
    40141 ggaattaatt tctgcagaaa aagctttgaa aacactattt tcacaagtaa ctgtaaataa
    40201 aaattataag ttacctaatg gtgatgaccg aaatatcaca atcgttagca agaaaaaaga
    40261 aacgcctaat aaataccctc gtaaagctgg tactccaaac aagaagcctt tgtaaagttg
    40321 ggagctagta tgaaacattt ctttgattac aagacaatgt ctgtcgcacg taaactcagc
    40381 attagtttta tcgctgttat cctattggga agtattttac tttcacttcc aatttttcaa
    40441 tatgctaacg cccctaaaac acattacatt gatcaccttt tcacgactgt ttcaatggta
    40501 tgtgtaactg gtttatctgt ttttcctatc agtaaggttt ataacggttg ggggcagatt
    40561 gtagctattt tactcatgca aacaggggga cttggtttag taacactaat gtcgttgtct
    40621 tactataccc taagacgtaa aatgtcgtta aatgatcaga cccttttgca gtcagcgata
    40681 acttataaca gttcgacaga tttaaaaaaa tatctctata tgatttttaa agtaacttta
    40741 actttagaag ttttagcagc cagcatttta gccatagatt ttattcctcg atttggtttg
    40801 agtcatggta tctttaatag tatattctta gctgtctcag ccttttgtaa tgccggtttt
    40861 gataatctag gggcaacgag cttagctcaa tttaaactaa atcctttggt taacattatc
    40921 gtttgtttct tgattatttc aggaggactt ggatttgccg tatggaaaga tttaattgaa
    40981 gctactatac aaacatctca caagggacca aaattaatca agacatttcc aaaacgcctc
    41041 agcaaccatt ctaaactggt tttaaaaaca acaaccatta ttttactaac aggaactttg
    41101 ctttcttggt tgttagagtt tggaaatttt agaacaattg ctaatttatc tctacctaaa
    41161 caactaatgg tcagcttttt ccaaacagtt acgatgcgta ccgctggatt ttcaactatt
    41221 gattataccc aaactgattt cgcaaccaac cttgtttata ttatccaaat gttaattgga
    41281 ggagctcctg gtggaacggc aggaggattt aaagtcactg ttatcgccat tttactctta
    41341 ctatttaaag ccgaactgag tggccaaagc caagtgactt ttcattatcg aacaattcct
    41401 tccagtatta ttaaacaaac actatcgatt ttaactttct tttttatcat acttattagc
    41461 ggctatctcc ttcttctgga actcaatccc catatagatc ctttttctct tttctttgag
    41521 gcatctagtg ccttagcaac cgtgggtgta acaatgaaca ctaccaatca attaactctg
    41581 ggcggtcgaa ttgtaattat gttcctcatg tttattggtc gagtcggtcc aattaccgta
    41641 ctgctatcaa ttcttcagaa aaaagagaag gaaattcact atgctgaaac agaaattatt
    41701 ctaggttaga aaaggtcttt tatgaaaaca aaaattattg gtgtacttgg tctaggaata
    41761 tttggacaaa cactcgcaca agaactaagt aactttgaac aagatgttat tgctattgac
    41821 agcaatcctg aaaatgtaca agctgtcgcc gaagttgtta caaaagcagc tatcggagac
    41881 attactgatt tagctttcct aaaacacatc gggatcagtg actgtgatac tgttattatt
    41941 gctacaggaa acagtttaga gagctcagta ttggccgtaa tgcactgtaa aaagttaggc
    42001 gtcccacaag ttattgctaa agctcgaaac cttgtatacg aagaagtact ttatgaaatt
    42061 ggtgctgatt tggttatctc tccggagcga gaatctgggc aaaatgttgc tgcaaacctc
    42121 atgagaaata aaattacaga tgtcttccag attgaatctg atatttctgt cattgaattt
    42181 aaaattccca aatcatgggt agggaaaact gtcgaacaat taaatatccg tcacaagttt
    42241 gatcttaacc tcataggtat cagaaaagca aaaaacaaac ctgtcgatac cgaagttcca
    42301 atcaatactc cacttgaaga aggtattatt ctagtggcta tcgctaactc agatgccttc
    42361 caacgctatg actatttagg atactttaac taaagaaaat aaaaaactga ctattaaagt
    42421 cagtttttta ttgccatggg ttatagaaac gtccatgatt aaggtgaatt aaataaaggc
    42481 ttacaaacag actagctaga gcaagtatga ttaagacaat gtcacttttt tccaaagatt
    42541 gcttagagta ccaagtacgt cttttatttt taccaaatcg tcgaagttcc atagcagttg
    42601 ctactgtatc aatacgttct agagagctaa aaattaatgg tgtgacaatc tgcaaattcc
    42661 ccttaatgcg tgctactaag ttcgattttt ttgataattc tattcctctt gcctcttgtg
    42721 ctcgacgtat tgtataaaat tcttcttgaa catcaggtat atatctaagg gtcaaactaa
    42781 cagcataagc tattttatat gataaaccta attgatttaa acttgatgcg aactgactag
    42841 gattagttgt catcaaaaac aaaagagcta aaggcactgt acaaaagtat tttaaaatca
    42901 gattaaagag gtaaaacaac tcttggctag tcacatcata tgggccaatt ccttttaaca
    42961 ataaagaaga actaccataa atcttatcac cataatgggg agcaaaaaga tagaccatta
    43021 taatatttaa aacagcgaat acaacaacta aagtcataac aaaggacacc tgttgccatt
    43081 tgatttttgc taatctaaat aagactagcg atcccaatga aacaacaatt aaaaatcttg
    43141 tatcataagt ggtcatgcaa gcaacagaaa ctaaaataaa aaatagtaac ttactggcac
    43201 ctgatagcct gtatagaaag ccataaccct cttgataacc aattaattgt ttagccattc
    43261 ttacctcctt gattatcaat gtaccattgt gttagtttct gtggagatat ccctaatctg
    43321 tctgctaaag caaataaact cgttttcttt aaatgagtct ttttcaaaag ctctgtattt
    43381 gaaaggatgt taataggatg atcatctgca acaatcagac cattgtcgat tacaattgca
    43441 cgatctgtat actctaacat aagctgcata tcatgtgtaa tcataacaat ggtatgtcca
    43501 tcacaactta gtttatcaag gaaagacatc atttcagtat aatgcttcat atcttgacca
    43561 gctgttggtt catccaaaat aataacttct ggatttaaaa tcagtatcga agcaatcgta
    43621 acacgttttt tctgaccgaa tgatagagcc gatataggcc aattcctaaa ttggtagagc
    43681 ccacaaacct ttaatatatc gtagacacga ctttctatat cattatcact aaaacctctt
    43741 aaacggagac ccagagcaac ttcatcaaaa accatcgctt gacttatcat ctgattggga
    43801 ttttgcaaaa catagccaat tcgttctgct ctttctttta tagagtcttg actgacatct
    43861 tcattattat aaattagagt agcattatta gggataaatt gacataacgc tttagctaaa
    43921 gtagatttac ctgcaccatt tttcccaacg atagctaacc gctgaccttt atataaatca
    43981 aacgaaacat catctaaagt attattctct aaatcatatg acaccgacaa atgctgtact
    44041 gataataatt tatctgtatg tttatctaaa tctaatgctc gcattttagg tatcaatagt
    44101 tcactgaaat caaagtcatc aatagacgtc attgtatttt gtttttcaaa gtcatatcct
    44161 aaatttttga gagcagaaat atacagtggt tctcgaatac catattcaga taaaaaatta
    44221 gtttttaaaa gttgatccgg ctccccatta taaataattt gtccatcact aaaaagaact
    44281 acacgatcta ctcgctcata aagaacatct tccaatcgat gttcaataat aatagtggtc
    44341 gcatcttgct cttgatgaat acggtcaacc aatgccatga tatcttgtcc cgattgtgga
    44401 tctagattag ccaaaggctc atcaaataat agaattgggc tatcgtctac taaaactcca
    44461 gctaaactaa cccgttgttt ttgtcctccc gacaagtctt gtggacgttt agacaaaaga
    44521 ggagctattt ctaaacgttt tgcccacatt tcaacgatag aggccatttc ttcctgtgct
    44581 acaacatcat tttctaatgc aaaagcaata tcttctgcta ctgtcaatcc tataaattgg
    44641 ccatctggat cttgtaaaac cgtagaaact aaatgagact tgtcatatat tgacaagtca
    44701 aaagcatttt tatggttaat cgtaaaaata ccactatatt ggcctttatg agtatttggg
    44761 ataataccat ttaaacaatg accaagcgtt gacttccccg accctgaagg tcctaatatc
    44821 aagacctttt cacctttagg aatgcttaag ttaatccctt ttaaagttgg ttcagattga
    44881 acgtcatatt gaaaggtaaa atccttccat tcaataaaat ctttcataga ttactctctt
    44941 atcctaatct ttggataaac tacctttttg tggtcgtgat ttagcgtatg ctagcaacaa
    45001 aagcgtacca cctactccaa tagtaatgga attcacaagg cttgataaga aaccttgtgc
    45061 aaaaactttg cttgcaggtt cgctataaaa gaaaatatca ccatacggtg caacgacaga
    45121 ccaacctata aggttagcaa taacttgaac tacattaaat agaagcaaat ccttcttaat
    45181 catgccagat aattgaatag ctaatttttt agcaaaaaag cctatcatca aacctaaaag
    45241 accactaact aaaacccaag tccaccacgg agaaccatat tgaatcgaat ctttcaatgc
    45301 gtgtccgata aaaccagtaa aaaaaccaac tcctggtcca taaataactg caaataatgc
    45361 taaaactgca tactgcaatt gaatattagt atttggaatt ggtgttggta tattaactag
    45421 catacctata atgataaaaa gtgcagctcc aattccagtc gctacaacct tttttattgt
    45481 gtttgtattc ataaaaacct ctctgaaaaa caagcttaaa cagtcactta caatctgttt
    45541 tattttatca tatttcaagc tgatttgaaa ccttattgac aagaagaact gaaaaacgat
    45601 tgaaaataat attaaatacc aaaaaagatc tcagatagaa tacctaagac cttcattagt
    45661 ttattttttt ccaaaaactg atttttcagc tacagccgtg aacagaacgt ctgtcgatga
    45721 atttaaagct gtctcacacg aatcctgtat cacccctaca atgaaaccaa ctcctacgac
    45781 ttgcattgca acgtcatttg aaataccaaa taagctacat gccaccggaa tcaataaaag
    45841 tgaaccacca gttacaccag aagctccaca tgctgataca gcagcaacca cacttaataa
    45901 gaatgctgtt ggaaaatcaa cagtaattcc taaagtattt actgctgcta aagtcaggat
    45961 attaattgta atagctgcac cagccatatt gatagctgca ccaagcggga tagatactga
    46021 gtaagtgtct ttatctaatc ctaaatcctc acatagtctc atattaacag gaatattcgc
    46081 cgcagaacta cgtgtaaaga aagctgtaat acctgaatcc tttaagcatc gtaataccaa
    46141 agggtaagga tttttacgca tcataacaaa agcaatgatt gggttaacga caagagctac
    46201 aaataacatc gtacccacca aaactaatat taaaagacca taatcaccta gtataccaat
    46261 accgttttct gaaatgctag tgaaaaccaa ccccataata ccaattgggg ccaaattgat
    46321 aatccatttg acaacttgtg aagtagcatc agcaaaagtt tgcattaaat cttttgtacg
    46381 ctgattactg cttcgcatcg ccaaaccaaa aataaccgcc caaaatagaa tactcatata
    46441 attggcctcg gtaatggcat gaatgggatt atcaacaatt ttcaacaaaa gagattgaaa
    46501 gacttctgaa atccccttgg gaggtaataa gtctgtcttt gctggcgttt tcaatatcag
    46561 tgtcaaaggg aaaatatagt tcacaacaac agcgattagg gccgctgcaa aagttgcagt
    46621 gatatacaaa ccaattatcg ttgacatatt ggtcttaccg ccctcacggt gttgcgacaa
    46681 ggcactaata actaaagtga aaactaaaag aggggcaata gctttcaacc ctcctacaaa
    46741 aagttgacca agtaaaccaa tcgcagaggc tttagggact aaaataccca agaataagcc
    46801 aataacgact ccaattgtta ttttttttat caagcttgtt tttctccaag cttttaaaaa
    46861 tcgtttcata ctaaactcct aaaaaattct taaagtctat gatatgataa aaaaaaagat
    46921 tcgtcaaata ccttttttat tttttagagg ttgacgaatt ctaaattttt ctgatataat
    46981 tactttttga aatattcaat caatttcgaa aataaaatat ttttttgtac agaaaggtac
    47041 attgatgaaa ctaaaaaaca cttatatcat tatcggcttg atgctttttg cgctgttctt
    47101 tggagccggt aatcttattt atccagccta cttaggtatc tactctggtt ctaattgggc
    47161 gtgggctatc attgggttct gcttaacagg cgtaacttta cccctacttg gtgttgcagc
    47221 tgtgacgtac tcaggagcaa ccgatgttga aagcttcacc aaacctgtct ctaaaacata
    47281 tgctattttc tttgcagtag cactatacct atctattgga cctttctttg ccattcctcg
    47341 aacaggggca acctcattct cagttggtat tcaacctatc ttaggcgaca gctccttagt
    47401 caaaattatc tatggtttaa ttttctttgg tatttcttat ttcttagcta ttagacctag
    47461 tcagattgta gatcgtattg gtaaatatct aactcccttt ttactaactg ttattagtat
    47521 tttaattatt gcctcactca tccatccagc tgctatcgtt ggaacaccac ataacgcttc
    47581 aacgaatgtc agcgatgctt tcaaggattt accattccta gcaggactga ttcaggggta
    47641 tggtacaatg gatgcccttg catcaattgc ttttgctatt atcgttattc aagcaagtaa
    47701 acagtacgga gcaattacca aaaaagaaat tacttctatg acacttaaat ctggtgctat
    47761 cgctacattt cttttagctt ttatttatat ttttgtagga cgtattggtg caacttcaca
    47821 aagcttgttt aaatttgcaa atggttcctt cttacttcac aatactccaa tagacggtgg
    47881 gcatgttctt agtcaatcag ctaacttcta tttaggaatt gtgggacaag ctattttagg
    47941 tacggctatt tttcttgctt gcttaacaac agctactgga ttaatcactg cttgtgcgga
    48001 atacttccac aaattacttc caaagattag tcacattact tgggctacaa tctttacttt
    48061 aattgctatt acgttctatt ttggtggttt atcagagatt atccgttggt cactaccagt
    48121 tctctacctt ctctatccat taactatagt cttaattttc ttagtctttt ttgatcaaaa
    48181 atttgaaagt agccgcattg tctaccaaac ttctattgcc gccactgctg tcgctgcttt
    48241 atacgatgcc ttatcaaaac tcggcgaaat gactggttta tttaccattc catccgcttt
    48301 gaccacattc tttactaaag ttgttccttt aggtgaatat tctatgggat ggattagttt
    48361 tgcaatttgt ggtgtcttag ttggtcttat tctaaaaaaa gtaaaagcat aataatttaa
    48421 agcagtacga aaattactgc ttttatttta aaataattat tgtaaccgct ttcttttggt
    48481 ggtataatag ccttgaaaat aaataatttt tggaggaact ctaatatgag agctgcaact
    48541 tatctttcag ctaatgaatt atcacttact gataaggcta aacctcaagt aataaaaccc
    48601 acagatgcgg ttgtaagact tgtcaaaaca accatttgtg gtactgatct acatatcctt
    48661 ggtggcgatg ttcctgcttg taaaccagga acaactttag gtcatgaagg cattggtatc
    48721 gtcgaagaag ttggttctgc tgtcaatcat tttaaagtcg gagataaagt aattatttct
    48781 tgtgtcactg cctgtaatac ttgctattac tgtaagcacc agttgccttc acattgtgaa
    48841 gatggcgggt ggattttagg acatcttatt gaaggtactc aagcagaata tgttcatatt
    48901 ccacatgcag atggtagcct ttatcatgca cctgaaggtg tttgtgacga tgcattggtt
    48961 atgttatctg atattttacc aacttcatac gaaatcggcg tcttaccttc tcacattaaa
    49021 ccaggtgata ctgtttgtat tgtaggcgct ggaccaattg gactatctgc cttactaact
    49081 gcacaattct attctcctgc caaaattatt atggttgatt tatctcaaaa acgacttgaa
    49141 gcctctaaaa aatttggtgc aacacacacc attctatcaa cgtctactca agaagttaaa
    49201 gaagaaattg ataaaattac caaaggccgt ggcgttgatg tcgtattaga atgtgttggt
    49261 tatcctgcaa catttgatat ttgtcaaaat gtagtttcta ttggaggaca tatcgcaaat
    49321 gttggtgttc atggtaaacc tgttgaattt aacctccaag atttatggat aaaaaatata
    49381 acccttaata ctggattagt taatgctaat acaactgaaa tgctccttga agttcttgag
    49441 acaggcaaaa tcgatgcaac acaactagta acacatcact tcaagctatc tgaaatcgaa
    49501 gaagcttata aggtatttaa agctgctgaa gaaaacaata ctctcaaagt tatcatcgaa
    49561 aacgatatta ctccagcctg ataattttta aacaaatgaa aatgtgagaa gaatagcctt
    49621 ctcacatttt tatttatgac tcaagcgacg tgttaaaaaa tcacctaaaa attggataaa
    49681 gaaaattaat aataaaatca gaatagtcgc tactaaagta atatcatcac gagaatagtt
    49741 atatccttta gtaatagcaa cagaacccaa tcctcctgca ccaatagcgc cagccatagc
    49801 tgtttcacct actaaagaaa tcaaagtaac cgttgatact cgaattaaat ctggtagacc
    49861 ttcacgaaga taaactacaa taatatccca aagtgttcca cctgaggctt gtgcagcctc
    49921 aataactcca ccatcaagtt cagctaaaac aacttgaact tgacgagcaa aaaatgggaa
    49981 aactgccaaa gaaagaggta ccaaagctgc tggtgaacca agtgttgttc ctacaattac
    50041 gcgagttact ggcgcaatca aagcaagaag aataatgaag ggcagagctc taaaaacaga
    50101 aacaacttta tctaaaactc caaatactaa tttattagca ataactcctc taggactagt
    50161 taaaacaagg aataaacctc ctaacaatcc cattaaacct ccaataagga acgaccaaaa
    50221 agtcatataa agggtttgta caatagctgt ctgccagccg taagcacctt cccaacccat
    50281 ttgatataca tttggtaaat gtgtttgaat ccactctatc atgctttacc accttctttc
    50341 aataacgtta attgaacttg cgaatctgta attgcttggc aggcttgtct taatttttct
    50401 tcgtcacctg ataagataac taccatttct ccaactggaa taccatctaa aatttcaata
    50461 ttcccataga ggatgttagc agttacttca aattctttat aaatttgatt taaaagcggc
    50521 tcatccgtgc tatgcccagc gtatttcagt tgaactaatt tgctacctac tggtagattt
    50581 ttgacaactt cttgttgctc aatcttaagc attgcctcat caatcccagt agctattttt
    50641 ataaaatctt gtgtaagact ctctctagga tgtgaaaaaa tgtctaaaac tgatccttct
    50701 tcaatcaatt taccattttg cataacagca acgcgattag caatgtcttt gacaatttgc
    50761 atctcatgtg taatcaaaac aatcgtaagt cccaattttt tattcagatc ttggagaagg
    50821 gcaagtattt gttttgttgt tttagggtca agtgcagagg tagactcatc tgaaattaag
    50881 attttaggat cgttagccaa tgcacgcgca atggcaacac gctgtttttg ccctcctgat
    50941 aattgtgaag gatagttttg agcacgatcg gataatccaa caagttctaa taatttagca
    51001 accttagctg ctttcgcctc ttttgataaa cctgaatgtt taagagcaaa agctacattc
    51061 tgttctgctg tcaactgtga cattaggtta aaatgttgaa aaatcattcc aatttcacgg
    51121 cgctgttcgc gtaattgtgt gctagtcagt gtcaccttgt tgtcgtaaat aacttggtca
    51181 tcaatagtaa ttttacctgc tgaaggttct tgtagcaaat taataacacg aacaagggta
    51241 gattttccgg ctcctgaata accgacaatt ccatatatat caccttgatt aatatggata
    51301 gtaacatctt taacagcatt aatctcacgt ttcttttggt ggaaagtaac atcaatgttg
    51361 tctaatttaa taattggttc tttactcata tgattctatt aaagcctcca ctaattcaat
    51421 atgggtgtaa taatcagcaa tactaacatt ttcatcaacg ccatggtctc gactattggc
    51481 atgtcctatg ccaatagctg caattggcgc tcttagagct tgatgaacta aatacattgg
    51541 tcctgtacct ggtgaggtag gtaacagaga tatcccttct ggataaaatt gctcagctaa
    51601 atcaacaacc tttaggatag atggcgctga catatcacta cgataagaca tttcaccaag
    51661 ggtataggtt agctcaatgt ctttaaagcc attctctttc aaatgatttt gtattgaatc
    51721 tagtacacct ttcggggtta accctggtac taacctaacc tctgctttac atttagcata
    51781 agcaggtaaa attgttttga cgccttcacc ttgataacct gaggttatac cttcaatggc
    51841 tatagaaggt tcaaaataaa gctttctcaa aaaagtttta tgactatctg ctaaactggg
    51901 caaaactaat tggtaagcac cttctagcgc ctttgcagat ctataagaat atttttcaac
    51961 taattctaac tctctcttag tagggggaat aactttatca taaatacctt ctaccaatat
    52021 atgacctttg ttatccctta acgtattgag ggcttgaatt aaataccatg tcgaggaatc
    52081 aacaacccca ccaaaggatg aatgaatatc aacatccgct gaacttactg ataaatcaaa
    52141 agtgacaatg cctttatttc caccagcaat ttccaactgt cctttaggat ttttagggcc
    52201 atcttcccac acgatcaaat cagccccttg tagctgctct tgatactttt ctaaataata
    52261 atcaaggcct actgaagctg attcctctgc tccttccaca ataaaagtga tatctagagg
    52321 aagttcccct ttgtgtcttg ataaatattt tttaactgct gataaacgtg cggtaatgtg
    52381 acctttatca tcatctacac cacgtccata catcttacca tatcgaagtg aaagtgtaaa
    52441 agggtcctca gtccactgtt ctacctcatc tgctggtaca gtatcataat gattgtaaaa
    52501 aataatacgc ttagcatcta cttttgatga tttgaaattt gccacgataa agggagcgct
    52561 atagctgtca tctataatga cttctgcgcc agcttttctg aaaacttctc caagatagct
    52621 tgataagtca tttaaaccaa ctttctgagc aaaaattgac ttaattgcaa ttaaatctct
    52681 taaaatattg atatcatcct gaacaatagt atcagataga aacttttgaa tttgattttc
    52741 ttcgtttgtt aaagtcatct ttcatccacc ttccttcaaa aacaatccaa gagcctggca
    52801 cattgtattc gtcccaggcc ctttatggct atatggctga taagttgtat cctattagga
    52861 ttccctgtta aacttataca gtcaattcac gttaccattg tggaatatca gctgaagtat
    52921 ctttgataac ttttttcact tcatctgtgt gataagcatc caagatagct tggatagctt
    52981 tagcgttctt ttgctttttc caatttttac gtcccgcaat gatattaatc cattgttttg
    53041 aatttttatc tgatttctca acaaagatag catctgaagg ttttaaatta gcttgctcaa
    53101 tgtatgtatt attaataata gctgcatcta catctttgag tgcacgtggt gtttgactcg
    53161 catctaactc ctgaatatta atatcctttt tattagatgt gatattagca actgttgcaa
    53221 ccttcttacc agaaacattc aatttgatta aacctgctga ctgaaggaca tacaatgcac
    53281 ggctaccatt tgttgcatca tttggaattg caatagtggc tccttttttc aattttttaa
    53341 gagattttac cttctcagaa tagatacgaa ttggagctaa gtaagtcttt tcaagtggaa
    53401 ttaagttttt cttattttcc ttattccagt tttctaagaa attgtaatgt tgaaaggcat
    53461 taatatccac atccttattg gctgtcgctt gatttggttg tgtataatct gtaaattctg
    53521 taaatttgat tttagcttta tcacctacta gcttttcaat tttatcccaa cgtgcttttt
    53581 cagtgtcaga aaaggtcata acaccaactt taacaacttt acttgaagtt gagcttgctg
    53641 aaacttcttg attactaaat gtgactccta aaagtgttgt taaggctaat cctaagacaa
    53701 tatgttttaa cttcataata aattccctca atttttgtga taaagatatt atcccatagg
    53761 aagttattag aatcaaattg ttataaactg tctcttgtta tagttttaaa ctatataaca
    53821 aaaaactaag gattgacatc tccttagttt ttattactac ttctccttag gagtaacgag
    53881 atctttctca tttggtacgt aatcacctcc aaggtattct ttagctattt ttgaaagtgt
    53941 cccatcttta cgtaattgtt taaggcgatt attcacatac ttttgcaagt ctttttgatc
    54001 ctgaccaaag atgaaataaa tataaggctg atctctcata gtcaaaggga tggtttttaa
    54061 attcgtgaga ccttgattct ttatgatagc gttaacagtt ggtccgtcaa aaagtttaaa
    54121 atcagctttt ccatcactca aattcgttag aatctgtgta atattttcat tagtatattt
    54181 tagagtaaca ggatttttct gatgctcttt attgaaattt tctaattgct tggcagttgt
    54241 cgttccttgg acaacctgtg ttttatgacc actaatatca ttataagctt tgatattact
    54301 attcttagga actgctaaaa ctgaaggagt agagcctatt gggtatgaaa ataaatattt
    54361 ttgagatctc tctgatgaat aagaaatatt atttccaccc atttgaaact ttcctgaatc
    54421 aatgccggta aataccgatg accattctgt ttttttaaaa gtgactttat agttatctga
    54481 acctttaaaa acagctttgg ctacttcaat atcaaagcca gtcagtttcc catccttcac
    54541 ataagaaaaa ggtgccgttg ttccaacagt tgcaaaatta acttcttttt tgcccgtctt
    54601 agtagtatga gattttgagc tacaagcagc tagagacgct actgtcacta aaataattcc
    54661 tgttacactt aacacttttt ttattgtcat cttttttctc ctctttaatt gtacacgaca
    54721 tcataacatc tttaagtttt taactaatac ttatcttcta tcgtctgaga gtttttgcta
    54781 ttatatggat gctacgtttc attaacaaaa taaccaaata aggtttggtt ttcaatttgg
    54841 tgaagcatta attctggatg ccattgaaca cctataatac attgttctgt tcgactttca
    54901 taagcttcta ctgtcccatc tctaggatct ctagcactaa ctatcagacc ttcagctaac
    54961 agtttaacac tttgtctatg aagagaattg atcttaaaat cacctgatat aaagccttct
    55021 agaaaactcc ctttttccac atttaaataa tgagcaaatc caatgtaagg ctcttgatag
    55081 tgattatcaa tagattgatt aagtgtacca ccaagcgcca catttactaa ctgcatgcca
    55141 cggcaaatcg caaagattgg tttattttgt ttcaacgctt cttcgactaa tgcaaattca
    55201 aaaatatcac gcgcaagtga ataatcatca ctctcaatta tcttctcttc accataatag
    55261 gatggtaaaa cattttggcc accagatatg atcagcttat ctatcatctc aacataagct
    55321 ttggcacttt ctgcttcact tattggtagg ataactggta acccgcctgc gtttttaact
    55381 ccttccgaaa tatgcctaga taccgaatca taataataac ctggaatatc tgacatttct
    55441 ctttcatttc ctgtaatacc aataataggc tttgttaaca ttttgctccc tcatcaacca
    55501 ataactgtct aataattatc attttacacc taaaaaaact ttttgaataa tattttttac
    55561 ctatcacttc tttaacaata cctatcaact ctcacaaaaa taaaaaccat agtattaata
    55621 ctacggtttt attttttaga aatctgctac attatgataa actttttgaa catcatcatc
    55681 tgattcgagt gcgtcaataa gcttttcaaa agtttcaagg tcatcacctt ccaatactac
    55741 ttctgattga ggaatcattt caagttcagt aacttggaat tcttctacac cattatcgcg
    55801 aagtgcttgg atacctttat gaagatctgt tggggcggta taaactgtta ttgttccctc
    55861 ttctgcttca acatcatcta cgtctacatc cgcttcaagt aattgttcga agacagtgtc
    55921 agcatcatca ccagcaaaaa cgatgacacc ttttttatca aataagtagg ataccgatcc
    55981 tgaagctccc atattgccac cgttcttacc gtaagcagta cgtacatttg ctgccgtacg
    56041 gttaacattt gatgtcaaag tatccacaat aatcattgaa ccatttggac caaaaccttc
    56101 atagcgtccc tctacgaaag tttcatctgt gtttcctttg gctttatcaa tcgctttatc
    56161 aataacatgc tttggaactt gtgcttgctt agcacggtcc aaaacgaatt ttagagctga
    56221 gtttgactcg gggtctggtt caccttgctt tgcagcaaca tatatttcaa caccgaattt
    56281 agcgtatact tttgagttag caccatcttt agcagtcttt ttggcaacaa tattggccca
    56341 cttacgtccc attggaaaat ctcctttgat ttcattttta ataacctgct ggctcatatt
    56401 ggcaagcaat cttaatttta actctattat tatagcactt tgatagcaat tggtaaaatg
    56461 gaaattctat cacggtatgc agtagaattt tttagaattg tcaattttct attaaggaga
    56521 acctaccgag ttccttttat aaaaagactg aacaaatttt cctttgccca gccttgacag
    56581 attcggtgtt atcctgtatc ttacgataaa gactaagtaa gggaacgttg taattggact
    56641 tactcggtaa agcgtagtgg ttaccataac tgcgcttctt ttattcgatt aattcaatag
    56701 aattgtagca cactacaaaa cgaataacga gctttttaaa gaaaaatata agaaaagccc
    56761 ttttaaaggt gcttttctta tatttgtttc aaatcaatca atttctttat ttctttaatt
    56821 tcgacatcct taaaaggaac cgttttttca aactcgcctc tattaacttg tttaaatcct
    56881 agatctctgt cattaccaat ttgtacatca atagtgcata actcaactat tttctctcta
    56941 ttttttcctt ctctaataaa aaattgtgta aatatatggt cacaatagaa gcaacatctt
    57001 tccacttgat acacctcact aatttcagtt tgatcaaccg gtcgtatgtg ataatattga
    57061 tatttgctga aacccttttg ccactttcta ctccatagga aaaatttatc ctgcttacta
    57121 tataacgaca cctcctttcc ttggaaaact gtctctccca catgagtcgc accatcaagt
    57181 ggtgcatctt tctcatcaaa cgaaatcttt ttcttagaca aaatcgacat tatcaactgt
    57241 gcagaaccta ttgcataaaa aattgttcct acaataaata ctagtaaaat tattgttagt
    57301 acaatgattc caaaaacttt ctgtgcatcc attgaggaat acccagacaa attatatacc
    57361 aatgtatatc cacctacagc agttattgat aggcaaataa aaaataatat caacacaccg
    57421 tacgaagaat ccatgatctt acatactaac ttttctgata gaaatttcca tacaaggcgc
    57481 acaacacttc cctccctaaa tatattaata taatagctat acacattaaa gtgaccatat
    57541 tcggatattg atcatctagt ctcatgtcat caaaaatcaa aaactcaata aaattctatg
    57601 ctacatggct ataattgaag taagctgagt tattttagat tcgtataaca ttacagtaga
    57661 tatatatatt gcaacaagca aagccctaaa aaatagtaaa cctttgtgat ttactatttc
    57721 taaaaatatt ctattcaatt aatcaagcta gaagctatcg ttctactttc taatgcaatt
    57781 ttctgtgaaa gttttcttca actcccgttc agtcgtaagt attaacacaa ttaaaatagt
    57841 tattcgtata gaaactacaa ttaagctaat aatacctatt tcatcatctg t
//
LOCUS       DATRSG010000006        57719 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRSG010000006 DATRSG010000000
VERSION     DATRSG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57719
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.006"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(308..694)
                     /gene="rplQ"
                     /locus_tag="VBD89_000301"
     CDS             complement(308..694)
                     /gene="rplQ"
                     /locus_tag="VBD89_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO7134857.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(709..1647)
                     /locus_tag="VBD89_000302"
     CDS             complement(709..1647)
                     /locus_tag="VBD89_000302"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO7134858.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1697..2080)
                     /gene="rpsK"
                     /locus_tag="VBD89_000303"
     CDS             complement(1697..2080)
                     /gene="rpsK"
                     /locus_tag="VBD89_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO7134859.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2098..2463)
                     /gene="rpsM"
                     /locus_tag="VBD89_000304"
     CDS             complement(2098..2463)
                     /gene="rpsM"
                     /locus_tag="VBD89_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO7134860.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2481..2597)
                     /gene="rpmJ"
                     /locus_tag="VBD89_000305"
     CDS             complement(2481..2597)
                     /gene="rpmJ"
                     /locus_tag="VBD89_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO7134861.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2623..2841)
                     /gene="infA"
                     /locus_tag="VBD89_000306"
     CDS             complement(2623..2841)
                     /gene="infA"
                     /locus_tag="VBD89_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO7134862.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2957..3595)
                     /locus_tag="VBD89_000307"
     CDS             complement(2957..3595)
                     /locus_tag="VBD89_000307"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO7134863.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTQ
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPTCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3690..4994)
                     /gene="secY"
                     /locus_tag="VBD89_000308"
     CDS             complement(3690..4994)
                     /gene="secY"
                     /locus_tag="VBD89_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO7134864.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5015..5455)
                     /gene="rplO"
                     /locus_tag="VBD89_000309"
     CDS             complement(5015..5455)
                     /gene="rplO"
                     /locus_tag="VBD89_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO7134865.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5580..5759)
                     /gene="rpmD"
                     /locus_tag="VBD89_000310"
     CDS             complement(5580..5759)
                     /gene="rpmD"
                     /locus_tag="VBD89_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO7134866.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5774..6268)
                     /gene="rpsE"
                     /locus_tag="VBD89_000311"
     CDS             complement(5774..6268)
                     /gene="rpsE"
                     /locus_tag="VBD89_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO7134867.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6287..6643)
                     /gene="rplR"
                     /locus_tag="VBD89_000312"
     CDS             complement(6287..6643)
                     /gene="rplR"
                     /locus_tag="VBD89_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO7134868.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6744..7280)
                     /gene="rplF"
                     /locus_tag="VBD89_000313"
     CDS             complement(6744..7280)
                     /gene="rplF"
                     /locus_tag="VBD89_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO7134869.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7390..7788)
                     /gene="rpsH"
                     /locus_tag="VBD89_000314"
     CDS             complement(7390..7788)
                     /gene="rpsH"
                     /locus_tag="VBD89_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO7134870.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7943..8128)
                     /locus_tag="VBD89_000315"
     CDS             complement(7943..8128)
                     /locus_tag="VBD89_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO7134871.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8146..8688)
                     /gene="rplE"
                     /locus_tag="VBD89_000316"
     CDS             complement(8146..8688)
                     /gene="rplE"
                     /locus_tag="VBD89_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO7134872.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8712..9017)
                     /gene="rplX"
                     /locus_tag="VBD89_000317"
     CDS             complement(8712..9017)
                     /gene="rplX"
                     /locus_tag="VBD89_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO7134873.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9097..9465)
                     /gene="rplN"
                     /locus_tag="VBD89_000318"
     CDS             complement(9097..9465)
                     /gene="rplN"
                     /locus_tag="VBD89_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO7134874.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9490..9750)
                     /gene="rpsQ"
                     /locus_tag="VBD89_000319"
     CDS             complement(9490..9750)
                     /gene="rpsQ"
                     /locus_tag="VBD89_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO7134875.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9776..9982)
                     /locus_tag="VBD89_000320"
     CDS             complement(9776..9982)
                     /locus_tag="VBD89_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO7134876.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(9992..10405)
                     /gene="rplP"
                     /locus_tag="VBD89_000321"
     CDS             complement(9992..10405)
                     /gene="rplP"
                     /locus_tag="VBD89_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO7134877.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGTPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10409..11062)
                     /gene="rpsC"
                     /locus_tag="VBD89_000322"
     CDS             complement(10409..11062)
                     /gene="rpsC"
                     /locus_tag="VBD89_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO7134878.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11075..11419)
                     /gene="rplV"
                     /locus_tag="VBD89_000323"
     CDS             complement(11075..11419)
                     /gene="rplV"
                     /locus_tag="VBD89_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO7134879.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11435..11713)
                     /gene="rpsS"
                     /locus_tag="VBD89_000324"
     CDS             complement(11435..11713)
                     /gene="rpsS"
                     /locus_tag="VBD89_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO7134880.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11812..12645)
                     /gene="rplB"
                     /locus_tag="VBD89_000325"
     CDS             complement(11812..12645)
                     /gene="rplB"
                     /locus_tag="VBD89_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO7134881.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCSATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12663..12959)
                     /locus_tag="VBD89_000326"
     CDS             complement(12663..12959)
                     /locus_tag="VBD89_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO7134882.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(12959..13582)
                     /gene="rplD"
                     /locus_tag="VBD89_000327"
     CDS             complement(12959..13582)
                     /gene="rplD"
                     /locus_tag="VBD89_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO7134883.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13606..14232)
                     /gene="rplC"
                     /locus_tag="VBD89_000328"
     CDS             complement(13606..14232)
                     /gene="rplC"
                     /locus_tag="VBD89_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO7134884.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14305..14613)
                     /gene="rpsJ"
                     /locus_tag="VBD89_000329"
     CDS             complement(14305..14613)
                     /gene="rpsJ"
                     /locus_tag="VBD89_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO7134885.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14821..16101)
                     /locus_tag="VBD89_000330"
     CDS             complement(14821..16101)
                     /locus_tag="VBD89_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7134886.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTSLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGILTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVRYHRYIQKM"
     gene            complement(16206..17696)
                     /locus_tag="VBD89_000331"
     CDS             complement(16206..17696)
                     /locus_tag="VBD89_000331"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO7134887.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKHGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQLINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRMVVPTSNMRETVEECLGLKQ"
     gene            complement(17816..18832)
                     /gene="adhP"
                     /locus_tag="VBD89_000332"
     CDS             complement(17816..18832)
                     /gene="adhP"
                     /locus_tag="VBD89_000332"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO7134888.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(19011..21653)
                     /gene="adhE"
                     /locus_tag="VBD89_000333"
                     /gene_synonym="adhC"
     CDS             complement(19011..21653)
                     /gene="adhE"
                     /locus_tag="VBD89_000333"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO7134889.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMKHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21922..23700)
                     /locus_tag="VBD89_000334"
     CDS             complement(21922..23700)
                     /locus_tag="VBD89_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO7134890.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLVSMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23697..24077)
                     /locus_tag="VBD89_000335"
     CDS             complement(23697..24077)
                     /locus_tag="VBD89_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134891.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24084..24521)
                     /locus_tag="VBD89_000336"
     CDS             complement(24084..24521)
                     /locus_tag="VBD89_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO7134892.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVSDGCKQWLTLINNSRD"
     gene            complement(24673..25671)
                     /gene="ruvB"
                     /locus_tag="VBD89_000337"
     CDS             complement(24673..25671)
                     /gene="ruvB"
                     /locus_tag="VBD89_000337"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO7134893.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNITEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            complement(25968..26879)
                     /locus_tag="VBD89_000338"
     CDS             complement(25968..26879)
                     /locus_tag="VBD89_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7134894.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYLILRIPTYADAERLKLRECQF
                     DHIFEEFYDNLPEDECLAIDSLQAKFEVYQTGDINFGVEVLCECFDKVKYKEKYTLND
                     LIIIDLFLTCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNHVLLNCVFV
                     GLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKSAENAYQQS
                     IMFSKMIDDKHLIKKLELEWQEDITGH"
     gene            complement(27032..28330)
                     /gene="purB"
                     /locus_tag="VBD89_000339"
     CDS             complement(27032..28330)
                     /gene="purB"
                     /locus_tag="VBD89_000339"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO7134895.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEKYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            complement(28348..29193)
                     /locus_tag="VBD89_000340"
     CDS             complement(28348..29193)
                     /locus_tag="VBD89_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134896.1"
                     /translation="MIQDILKSVSREDSNTLETDTLAIKDNAIIVKNSSIQITSISMV
                     SRSNFSSPITLLDYVILFVLFIIGLIPPFIGLIFLVGYAYLVYKKHKQHLNSKYYIIF
                     NLGSSRDYFLYFKDSNFRDKVFDVITKSFNNKNQNIKIDIKNQNIKGDNYESGSSRND
                     VSGSGNIVGTNIDNGSQVSLSGDISNGSTIIKDSNINNADIHKSVEIPWTDMVEQLNL
                     IINTQLSTADKAIYSELLEISEAEDERKLKNFVNKMSSFFKNDYVQNTITGVLGNVIS
                     SILMK"
     gene            complement(29325..30569)
                     /locus_tag="VBD89_000341"
     CDS             complement(29325..30569)
                     /locus_tag="VBD89_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO7134897.1"
                     /translation="MGLFGFGKPKDEALEAKISSLESKLKDKQSILDDLTRQIKVAKD
                     ILKLDDELKKRQSELKALQKEVIVLNDDLDLQEYGFFERKYKFSDSTKYKEELETLRK
                     RQKQLVKDKKAGVIVRPMLLDNSKSKGRAMQNQLIKAMIRGFNGEVDAHLVKVTATNL
                     SKKVDAVVKSFNQLNKMYSRNLVCLSNDYLELKISELQLAVEYELQKLEEKELLREQR
                     AKEREDKKLQAELAAKRKQIEKDRKHFKQMLDNVEELLKTANEEEKEKLNLQLAEYQD
                     KLSELDELEEDIDYREGHATAGYVYIISNIGAFGEDVYKIGVTRRLDPMERIRELGSA
                     SVPFQFDVHALIFSEEAFSLEKELHNRFEKNKINKVNGRKEYFKVPFSDIKAVLEEHK
                     ELAIELTEKAEAFEYRQSLMTK"
     gene            complement(30579..31652)
                     /gene="purK"
                     /locus_tag="VBD89_000342"
     CDS             complement(30579..31652)
                     /gene="purK"
                     /locus_tag="VBD89_000342"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO7134898.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHDPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFVEDADEVEEF"
     gene            complement(31639..32127)
                     /gene="purE"
                     /locus_tag="VBD89_000343"
     CDS             complement(31639..32127)
                     /gene="purE"
                     /locus_tag="VBD89_000343"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO7134899.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            complement(32413..33675)
                     /gene="purD"
                     /locus_tag="VBD89_000344"
     CDS             complement(32413..33675)
                     /gene="purD"
                     /locus_tag="VBD89_000344"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO7134900.1"
                     /translation="MKLLVVGSGGREHAIAKKLLVSKGVDQVFVAPGNDGMTLDGLGL
                     VNIVISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTYSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKCAY
                     DGDMGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPILEGMITEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENGKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            33957..34760
                     /locus_tag="VBD89_000345"
     CDS             33957..34760
                     /locus_tag="VBD89_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO7134901.1"
                     /translation="MTIYDQIESALDLMTDLEHEIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(34783..35760)
                     /locus_tag="VBD89_000346"
     CDS             complement(34783..35760)
                     /locus_tag="VBD89_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO7134902.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     SQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(35768..36649)
                     /locus_tag="VBD89_000347"
     CDS             complement(35768..36649)
                     /locus_tag="VBD89_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7134903.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEINRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTIIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(36666..37583)
                     /locus_tag="VBD89_000348"
     CDS             complement(36666..37583)
                     /locus_tag="VBD89_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO7134904.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAMGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(37580..38242)
                     /locus_tag="VBD89_000349"
     CDS             complement(37580..38242)
                     /locus_tag="VBD89_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO7134905.1"
                     /translation="MKKANQLIAAIFDLNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVPFLKIICYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(38262..38705)
                     /locus_tag="VBD89_000350"
     CDS             complement(38262..38705)
                     /locus_tag="VBD89_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO7134906.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGYEVIKLGKGDKVEVEEYLGDIGFVK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            complement(38718..39548)
                     /locus_tag="VBD89_000351"
     CDS             complement(38718..39548)
                     /locus_tag="VBD89_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO7134907.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(39558..40445)
                     /locus_tag="VBD89_000352"
     CDS             complement(39558..40445)
                     /locus_tag="VBD89_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7134908.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(40533..41849)
                     /locus_tag="VBD89_000353"
     CDS             complement(40533..41849)
                     /locus_tag="VBD89_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7134909.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(41896..42594)
                     /locus_tag="VBD89_000354"
     CDS             complement(41896..42594)
                     /locus_tag="VBD89_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO7134910.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLATLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPKQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            complement(42841..44145)
                     /locus_tag="VBD89_000355"
     CDS             complement(42841..44145)
                     /locus_tag="VBD89_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7134911.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVFVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQVAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQL
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASAKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(44292..45191)
                     /locus_tag="VBD89_000356"
     CDS             complement(44292..45191)
                     /locus_tag="VBD89_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO7134912.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(45384..46931)
                     /gene="purH"
                     /locus_tag="VBD89_000357"
     CDS             complement(45384..46931)
                     /gene="purH"
                     /locus_tag="VBD89_000357"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO7134913.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGSPEMMDGRVKTLHSNIHGGILARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTNDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEVTALEFAWKAIKYVKSNGIIITNDHMTLGLGVGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(46951..47703)
                     /locus_tag="VBD89_000358"
     CDS             complement(46951..47703)
                     /locus_tag="VBD89_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7134914.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRVIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVIYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            complement(47726..48274)
                     /gene="purN"
                     /locus_tag="VBD89_000359"
     CDS             complement(47726..48274)
                     /gene="purN"
                     /locus_tag="VBD89_000359"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO7134915.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPDVLDSLGIKRK"
     gene            complement(48441..49463)
                     /locus_tag="VBD89_000360"
     CDS             complement(48441..49463)
                     /locus_tag="VBD89_000360"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO7134916.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVSRTERAGVMGALGGFG
                     GVFDLSKTGVREPILVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVQAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(49491..50945)
                     /locus_tag="VBD89_000361"
     CDS             complement(49491..50945)
                     /locus_tag="VBD89_000361"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO7134917.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEETSFYIQKVK"
     gene            complement(51179..54904)
                     /locus_tag="VBD89_000362"
     CDS             complement(51179..54904)
                     /locus_tag="VBD89_000362"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO7134918.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFDQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            complement(55027..55731)
                     /locus_tag="VBD89_000363"
     CDS             complement(55027..55731)
                     /locus_tag="VBD89_000363"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO7134919.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(55855..56094)
                     /locus_tag="VBD89_000364"
     CDS             complement(55855..56094)
                     /locus_tag="VBD89_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7134920.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(56105..57097)
                     /gene="plsX"
                     /locus_tag="VBD89_000365"
     CDS             complement(56105..57097)
                     /gene="plsX"
                     /locus_tag="VBD89_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO7134921.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DIVVTDGFTGNAVLKTMEGTAMNIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(57175..>57719)
                     /locus_tag="VBD89_000366"
     CDS             complement(57175..>57719)
                     /locus_tag="VBD89_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7134922.1"
                     /translation="IYICHWLSGFVEISSSNSFSFGPFFFLLLAYFIQSLTEEILFRG
                     YVMTTVTKFKGSFAGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNI
                     LFVTGVHTAWNFTMGCVLGNKVSGGYSPVSLFRITENSSFAIWSGGDFGFEGGVLCSL
                     ILVGCLAYFSLERVKVSVRY"
BASE COUNT        17371 a        12227 c         9893 g        18228 t
ORIGIN      
        1 attttataaa tttagtatag acataactat tcctctatat tcaattaaga gaaattatat
       61 aaccactatt gagaaatgta gtctagctat taaactcttt gctcatcgaa aatatccaat
      121 aaatataata cataaaacgc ctgcttacga aatataaaca aaattgtttg catttcgtaa
      181 acaagcgtta cctatttaac aatatatgat gagtgttccc gctgagaata attctcagcg
      241 gtagaccaga gctagactaa gaatcgattg attccatcat cataacactc aacaaaattg
      301 ataaaaatta tactaattca ataattgcca ttggggcagc atcgccacgg cgtggttctg
      361 ttttaagaat acgagtgtat cctccgttac gttcagcata acgaggtgcg atgtcgtcaa
      421 aaagtttttg aagagctgtt gttgatgtat atttatcaga agcttcatca tagttttctg
      481 atgcaatttc attacgtaca taagcagcag cttgacgacg agcatgtaaa tcaccacgtt
      541 tacctagagt aatcattttt tcaactgttt tacggatttc tttagcacgt gcttcagtag
      601 ttacaattga ttcgttgata agaagatcgg ttgtcaaatc acgaagcatt gccttacgtt
      661 gtgagctagt gcgtcctagt ttacggtaag ccattatgtc ctcctatttt atttatcgtt
      721 ttttaatcca agacctagat cggcaagttt gattttaact tcttcaagac tcttacgtcc
      781 taagtttcgg actttcatca tttcaggctc agttttttct gttaaatcaa atactgtatt
      841 aattccagca cgttttaaac agttatatga gcgcactgac aaatcaagtt cctcaattgt
      901 gcggtcaagc actttctcat cgttcacttt ctctgtttcc ttcattactt cagttgcttt
      961 agcaacctct gttaaatcag taaacaagtt taagtgttca attaagacgc gagctgaaag
     1021 accaagagca tcctcaggaa tgattgtgcc atttgtcatg atttcaatag ttaatttatc
     1081 aaagccatca ttactaccta cacgagcagg ttcaacttga taattaactt ttttcactgg
     1141 tgtatagatt gaatctacag ccaatgttcc tactggtgca tcatcttttt tatttccttc
     1201 tgctggaaca taacctcggt ttttagcaac ggtcattgtt gctttcaaac tgtgaccttc
     1261 agcaattgtg aaaagataat ggtcagggtt tacaatttcg atatcactat ctgttaaaat
     1321 atctccggca gtaatttctg caggaccttc aacgtctagt tcgataatct tttcgtcttc
     1381 gacgtaagat ttcacagcaa gtccttttac attaaggatg atttgcatca catcttcacg
     1441 tacacctggg atagtatcaa attcgtgtaa tactccatca attttgattg atgtgactgc
     1501 tgcacctgga agtgaagaca gaagcacacg acgaagagag tttcctagag ttgtaccgta
     1561 gccacgttct agtggttcga tgacaaatct gccgtaatct ttattttcat caatttttgt
     1621 tattattggt ttttcaaact caatcattta gttactccct cttaaacgaa aagttgtgta
     1681 ctataaaatg ctatgattat acacgacgac gttttggagg acgagcacca ttatgtggta
     1741 caggagtcac atcacgaatt gcagttactt caagaccagc tgcagcaagg gcacggatag
     1801 cagattcacg acctgaacca ggacctttta cagtaacttc aacagtttta agaccgtgtt
     1861 cttgtgcaga tttagcagca gcttcagcag ccatttgagc agcaaatgga gtagatttac
     1921 gagaaccttt aaatccaaga gcaccagctg atgaccatgc aagagcgtta ccatgcacat
     1981 ctgtaatcat aacaatagtg ttattgaatg tagcgtgaat atgagcaaca ccagattcga
     2041 tgttcttttt cacacgacgt ttgcgtgttg gtttagccaa ttgttttacc tcctatttta
     2101 ttttttctta cctgcaatcg caacagcttt acctttacga gtgcgagcgt tatttttagt
     2161 attttgtcca cggacaggaa gtccacgacg gtgacggatt ccacggtatg aaccgatttc
     2221 catcaaacgt ttaatattca agtttacctc acgacgtagg tcaccttcaa ctttgattgc
     2281 atcaacttca cgacggatag cgtcctcttg atctggtgtc aaatctttaa cgcggatatc
     2341 ttcagaaata cctgcagctg ctaagatttt tttagatgta gaaagaccaa ttccgtatac
     2401 ataagtaagt gaaattacta cacgtttgtc atttggaata tcaactccag caatacgagc
     2461 cattttatct cctttctata ttatccttga cgttgtttgt gttttggatt tgtcggacaa
     2521 attaccataa cacgaccgtt acgacgaata actttacagt attcgcaaat tggtttaacc
     2581 gatggtctta ccttcatgtt ttaatccctc caattatttc gactatttaa agcggtatgt
     2641 gatgcgtcca cgtgttaaat cgtatgggct catttcaaca gtaacacgat cacctaccaa
     2701 aatacggatg tagtttttac ggatttttcc tgatacagtt gccaaaattt ggtgtccatt
     2761 ttccaactca acagtaaaca ttgcgttagg catagtttca acaactttac cttcaatttc
     2821 aatcacatct tcttttgcca cgcaaaagta ccccctaaat ttcgatttga tgtcctctat
     2881 aaagaggtaa caattataag tcagactaac ttagtatatc attactaatc tacttatgca
     2941 agtagattag cataatttat ttgagttcta gcaacgcttt ttcaacatct gcaaaaactt
     3001 ctgttatttc ttgattacct tcaatatctg taacaagacc aagcttacga tagtgttcaa
     3061 gaataggttc tccttgagca atattaacgt ccaagcgacg tttgacagtt tcaggcttat
     3121 catcttcacg ttgatagtaa tcttcttctt tataatctac tggtgggttg aacactttgt
     3181 ggaaagtttc accagtttta cgattgataa tacggccact caaacgctct ataaggcatg
     3241 ttggatccac tttaatatta ataacaccat ctaagcgtag tcctagttct tcaagcgtag
     3301 catctaaggc gtgtgcttgc tcaatagtac gtggatatcc atcaagtaaa aaaccttttt
     3361 ctgcgatatc atcctcagct aagcgctctt ttacaatccc gtttgttact tcatcaggaa
     3421 ccaattcacc tttatcaata taacttttag ctaaacgtcc catttgggtt tgattagcca
     3481 ttgcggcgcg gaacatatcc cctgttgaga tgtgagcaac accaaattct tcaacaatct
     3541 tagctgcttg agtaccttta ccagcaccag gcaaacccat aattaaaaga ttcattatag
     3601 tctccttatt ttgtttttaa ataaatccaa cacagcatgt cggacttatt aaaaaacaaa
     3661 atagaaggtt gacagagtca acctatattc tattctgttg tattcatgaa tccaacatag
     3721 cgtctcttca aaagatagcc ctcaagttgt ttcatacctt caatacctgt tgcaatcaaa
     3781 atgagtaagc ttgtaccacc taacgcaata cttgatgaca aatgcaacgc ttgttgagca
     3841 ataattggta acaaggaaat aaatgctaga aatactgaac caattgttgc taattttttc
     3901 aatagcgatg acatatactc ttctgtttct cgtcctggtc gaatacttgg aatgtaggac
     3961 gaattttttt gaagattctc tgctgtcttt tcagggttta cttgtacaaa agtatagaag
     4021 aaagagaaca gaataatcaa gatagcatag ataatcatac cgactggggt ttgataatta
     4081 agtaattctt ggagtttagt aagccaagga atttctttac cattttgaaa gaaaggaata
     4141 atcgtacttg gaattgttgt aatcgagcta gcaaagataa cgggaataac accagctgga
     4201 tttactttta gaggaagata tgaacttgta ggagctcctt gaacaagttt tgtatattgg
     4261 attggaattt tgtattcagc ttgttgaata aatgttgtaa aaaatacaat tgctaatact
     4321 gctacaataa gaatcccgac aaagatgtaa gaatttgtaa ttgctgaaga gcgtacattt
     4381 acaaagaaat cttcgtagat agttgtaatt gcacttggaa tggaagaaat gatccccgca
     4441 aagataatca tagacactcc atttccgaat cccttatcag taatttgttc acctagccaa
     4501 gtaaccacca tactacctgt tgtaaggatt gcaccaataa gcaaatatgt ttgtacattc
     4561 ggagttttaa ctaaagcaac acttgataga gtgttaaaac cagcagtaat accaattgat
     4621 tgtacgaatg caaggaacaa agaaatataa cgtgtagctt ggttaagctt acgacgacct
     4681 acctctcctt gttttcccca ttcaacaaac ttcggaagaa tatccatttg aagtagctga
     4741 acaacaatag acgcagtaat gtatgggcta actcccatag agaataccga aaagtttctc
     4801 attgcattac cactaactaa gttaagcata tttagaaatg gaagttcacc catttgttct
     4861 aaactcttaa cattgatgcc cggtactgtg atatgcgttc cgatacgaaa tacaagaagg
     4921 ataaagatag taaataaaat tttatttctt accatcttta ctttgagtgc atctctaagt
     4981 aattttaaaa acataatgag ttacctctca ttaattagat gacttcgatt gaaccacctt
     5041 tagcagtgat agctgcttca gcagatttag aaaatttagc tgctttaaca gacaatttct
     5101 tggttaattc accattacca agaattttaa cgcctgattt ttcagcacgc acgattccag
     5161 cttctttaag aacaactgga gttacttcag taccatcttc aaagacattt aattggtcaa
     5221 ggttcacaag agcatattct tttgcattga tgtttgagaa cccacgtttt ggcatacgac
     5281 ggaacaatgg agtttgtcca ccttcaaaac caaggcgtac tccaccacca ctacgcgctt
     5341 tttgtccttt ttgaccacgt ccagaagttt taccattacc tgaagatgta ccgcgaccca
     5401 cacgattgcg gactttacgt gatccttctg caggttttaa ttcatgaagt ttcattattt
     5461 tttctcctta tttgtaaaat gctaacgcct ttacgagggg aaaggtactc cccgcaaaga
     5521 ctcgcctata cgatttgttt aaatcgcacc aataacaatc agtgcgattt agcttttaat
     5581 taagcttctt caacagttac taagtgagag atagcgttaa ccataccacg gatggcagca
     5641 ttatcttcct taacaactga agagtttaat ttaccaagtc caagggcaac aactgtttta
     5701 cgttgttctg gtttgcgacc gattggagac ttagtcaaag taattttaat ttgagccatt
     5761 ataatcccct ttcttatgct aagtcagaaa cagaaatgcc acgtaaagca gcaacttctt
     5821 ctgcacgttt taattgtttc aagccctcaa cagttgcgcg aacaatgttg attggagtgt
     5881 ttgaaccaag tgatttagat gtaatatctg caacacctgc taattcgatg acggcacgaa
     5941 cagcaccacc agcggcaacc ccagcaccct caacagctgg tttcaacaat actttcgctc
     6001 caccaaattc tgaacgaact tcgtgaggaa ttgttgtacc gaccatagga acttcaacca
     6061 tgtttttctt agcagcttca acagctttgc gaatagcttc tggtacttct tgagctttac
     6121 ctgtaccaaa accaacacga ccattacggt caccaacaac tacaagagct gcaaaacgta
     6181 gacgacgtcc acctttaaca acttttgtaa cgcggttaat cgcaacaacg cgctcttcaa
     6241 gttctactgc attatcttta aatgccatta ttaagtgtcc tccctattag aatttcaatc
     6301 cgttttcacg agctgagtca gccaaagctt ttacacgtcc gtgatagaga tatccaccgc
     6361 ggtcaaacac cacttcagaa atacctttag ctactgcgcg ttcagcaaca agtttaccga
     6421 caacaacggc ttgttctgtt tttgttccgt tagaaacttc tttatcaaga gttgatgcac
     6481 ttgcgagcgt tacacccgct acgtcatcaa ttacttgagc gtagatgcct gtattagaac
     6541 ggaaaatgtt caaacgtggg cgatcagcag ttccagagag tttaccgcga acgcgacggt
     6601 ggcgtttttg gcggatttta tttttatctg gtttcgaaat cacaattttc acctcttaaa
     6661 tatattgtta tgtcaaaatg acaatattaa aatgaaaaga tagagaattt gcagaaaaga
     6721 ttctacaaaa tctatcaatc ctattattta cctgttttac cttctttacg gcgtacgaat
     6781 tcaccaacgt aacgaatacc tttaccttta tatggttctg gtgaacgtaa gctacgaacg
     6841 taagcagcag tttgtccaac gctttctttg ttgataccga ttacgttgat tgttgttgga
     6901 gttggcactt caaaagtaac accttctgga gcttcaactt cgtcttggtg agacttacca
     6961 acagaaagaa caagttttga tccttgaagt tgtgcacggt aaccaacacc gcgcatttca
     7021 agagcttttt tgaaaccttc agaaacacca acgaccatgt tgttcaagtt cgcacgagtt
     7081 gtaccatgaa ttgttttcat ttcttttgag tcgttaggac gagtaactgt tacttcagtc
     7141 ccttcaacag tgattccaat atttttatta aactcacgag tgagttcgcc tttaggtcct
     7201 tttacagtaa caacattatc cttgttaatg atttcaacac ctgcaggcaa tgtaattact
     7261 ttattaccaa tacgtgacat gtttattttc tcctgttaaa ttgtcaagct gcttgagcaa
     7321 ctagttttca cggggactca tgagaccaca ggattatact gtgactcaat gagcatttta
     7381 ggtaaaattt taccaaatat atgcaagtac ttctccacca acattttttt ggcgagcttc
     7441 tttgtcagtc aaaagacctt cagaagttga tacaattgca ataccaagtc cgttaagaac
     7501 tttaggcata tcttcatgtt tagtataaac acgaagacct ggttttgaaa tacgtttcaa
     7561 gttagtgata acacgttcac cattttgtcc atatttaagg aacacacgga tgatgccttg
     7621 tttgtcatct tcaataactt caacgttttt tacaaaacct tcacgtttaa ggatatcagc
     7681 aatccctttt ttaatgtttg atgcaggtac ttctaacact tcatgttttg cttggttagc
     7741 attacgaata cgagttaaaa agtctgcaat tgggtcagtc ataaccattt tttaattctc
     7801 ctcttattag cagtttgaat ttaatcactt gctaattaat gagtccttac ggacgttgta
     7861 agactaggtc ttaatatatt tgcgtttaaa aatcagtgcc attatcaatg acacctttta
     7921 aacacaaagc atcattaatt tattaccaag atgctttagt tacgcctggt acttgacctt
     7981 tataagccaa gtcacggaag caaacacggc aaagttggaa tttgcggtaa actgagtgtg
     8041 gacgtccgca tttttcacaa cgagtgtaag cttgcgtaga gaacttcgct gggcgtttgt
     8101 tcttagcaat catagatttt ttagccaatt tatttacctc ctagattact ttgcaaaagg
     8161 cattccaagg ccttttagca attcacgtga ctcttcatca gtgttagcag tagtaacgat
     8221 aacgatatca agaccacgta ctttatcaac gtcatcaaag ttgatttctg ggaagatcaa
     8281 ttgttcttta actccaagag tgtagtttcc acgtccatca aatgatttcg ttggaacacc
     8341 atggaagtca cgtacacgtg gaagtgaaac agaaactaat ttatccaaga attcgtacat
     8401 acgttcacca cggagggtaa cttttgcacc gatcgctaca ccttcacgaa gacggaagcc
     8461 agcgattgat ttcttagctt tagtaataag tggtttttga cctgaaataa gtgctaactc
     8521 agcagcagct ttctcaagat ttttagcgtt tgatacagca tcaccaacac ccatgttaag
     8581 aacgattttc tcaacttttg gcacagccat aacagatgaa tagttaaatt tttctgtcaa
     8641 cgcaggaaca acttcattag tatatttttc ttttaagcga tttgccatta tgcttctcct
     8701 ttccttcgtg attaatcaag cacttcgcct gattttttgt tgtaacgaac ttttttgccg
     8761 tcaacaactt tgtaaccaac acgtcctgct acaccatttt tatcaagaac ttgaacgttt
     8821 gacacatgga ttggagcttc tttttcaacg atagcgcctt gagggttttc gttatttggt
     8881 ttttgatgtt tcttgatgag ggctacgcct tcaacaacaa ctttgtttac ttttggaaga
     8941 gctttaagaa ctacagcttc agtgcctttg tccttaccag caataacgcg aactttgtcg
     9001 ccttttttta caaacatttg agtttttctc ctatttttct tacgcctcga aaggcaccct
     9061 aggaaatcct aggggactac gtttgttaat ctgagattaa agtacttctg gtgccaatga
     9121 aacgatcttc atgtagccac cttcacgcaa ttcacgtgca actgggccaa agatacgagt
     9181 tccgcgagga gttttatcgt cacggatgat tacagcagca ttgtcgtcaa atttgatgta
     9241 tgaaccgtct gggcggcgtg caccagtttt tgtacgaacg ataacagctt taaccacatc
     9301 acctttttta actgctccac caggagtagc ttgttttaca gaagcaacga ttacgtcacc
     9361 gatgttagcg aatttacgtc ctgaaccacc aagtactttg atagtcaaga tctcacgagc
     9421 accgctatta tcagcaactt tcaagcgagt ttcttgttga atcatttcaa ttttctcctt
     9481 ttagtttgat tagataataa cagctttttc cactacttcc acaagacgga aacgttttgt
     9541 agctgaaagt gggcgagttt ccatgatacg aacgatatcg ccttctttag caacgttgtt
     9601 ttcatcatgt gctttatatt ttttagaata gttgatacgt ttaccataga ctgggtggtt
     9661 acgtttagtt tcaactacaa ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc
     9721 gtaaagagtt ttacgttgat tacgttccat ttagaatttc tcctttccca atctattatt
     9781 tcatttctga ttgcacagtc ttaacacgtg caatttgttt cttaacttcg tcaaggcgag
     9841 cagtcttttc aagttgacct gctgcagctt ggaaacgaag atcaaacaat tcttttttga
     9901 gttcgttttc tttcttagca agttcttctt gagacaatcc acgaagctct ttaacaaaat
     9961 ctttaatttc ttgaagtttc atgtcttctc cttattctgc ttcacgtttt acgaatttgc
    10021 atttaactgg taatttgtgg ctagcaagac gtaatgcttc acgtgcaact tcttcagaaa
    10081 caccagcaat ttcaaacatc actttaccac gtttaactgg tgctacccaa ccttcaggtg
    10141 tccctttacc agaacccata cgtacaccga tagcttttgc agtgtatgat ttgtgtggga
    10201 agattttaat ccaaacttta ccaccacgtt tcatgtaacg agtcatcgca atacgggcag
    10261 cttcgatttg acggtttgta atccagtggc tagttgtagc ttgaagtccg tattcaccga
    10321 atgaaacttc tttaccacct ttagcctcac cgcgcatttt tccacggaat tcgcgacggt
    10381 gtttaacacg tttaggtact aacatttgtt atttgcctcc tttagtgttt ttacgagctg
    10441 gaagaacttc tccacggtaa atccaaactt taacgccaag tttaccataa gttgtgtcag
    10501 cttcttccca agcatagtcg atatcagcac gaagcgtgtg aagtggaaca gttccttctg
    10561 aataaccttc agcacgagcg atatcagcac cgttcaaacg accagaaact tgagttttaa
    10621 tacctttagc tcctgcacgc attgtacgtt ggattgcttg tttttgagca cgacggaaag
    10681 caacacgttg ctctaattga cgagcaatat tttcaccaac aaggtgagca tctaaatcag
    10741 gttgtttgat ttcaatgatg ttgatgtgta cttgttttcc agttaatttg ttaagttgac
    10801 cacgaagagc gtcaacgttt gctccacctt taccgataac catacctggt ttagcagtgt
    10861 gaagtgaaac aataacttta tttactgcac gttcaatttc aatagttgaa actgatgcgt
    10921 cagctaattc tttattaatg aatttacgga ttgcaagatc ttcatgaagg taatccgcgt
    10981 attctttttc agcataccat ttcgcatccc agtcacggat gattccgaca cgcataccaa
    11041 ttggatgtac tttttgaccc acgattttac ctccttattt ttctgataca actacagtta
    11101 cgtgagttgt acgtttgttg attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt
    11161 tcattgttgg tccttcgtta gcgaaagttt cagatactac caagtttgct ttttccaaac
    11221 caaagttgtt ttctgcattt gcaattgctg agttaagagt tttctcaata acacgagctg
    11281 ctttgtttgg agtgaatttt aagattgcga ttgcgtcagc aacattctta ccacggataa
    11341 gatcaagtac taaacgtgtt ttacgaggtg aaacacgcac tgtacgagcc attgctttag
    11401 ctgaagtaat ttctgccatt gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag
    11461 ctgcgtgacc tttgtaagta cgtgttggtg caaattcacc aagtttgtga cctaccatgt
    11521 cctcttggat ataaacagga acatgtttac gtccatcata aactgcgatt gtgtatccga
    11581 tgaaacttgg gaaaatcgtt gaacgacgtg accaagtttt gattactttt ttcttttcgt
    11641 catttgcttg agcttcaact tttttcatta aatgctcgtc gacgaaaggt ccttttttaa
    11701 gactacgtcc cattttgtag ttttctcctt taaataatgt accacaacgg cctgtacact
    11761 atagtgtact accgagctgg cggatttgta taagattatg ctattaacct attatttttg
    11821 gttacgacga cgaacaataa gtttgtctga tttagctttc ttattacgag ttttaagtcc
    11881 aagcgcaggt ttaccccatg gagtagatgg tgctttacga ccaactggtg ctttaccttc
    11941 accaccaccg tgtgggtgat cgttagggtt cattacagaa ccacgaactg tagggcgaac
    12001 acctttccaa cgattacgtc ctgctttacc aatgttaaca agtgattgct gttcattacc
    12061 aacagtacca attgttgcac tgcaagtacc aaggatcata cgtacttcac ctgattgaag
    12121 gcgaacaaga acatatttac cttcttgacc aagtacttga gcagaagcac cagcggcacg
    12181 aatcaattca gcacctttac ctggttgcaa ttcaatatta tggataactg taccgactgg
    12241 aatgttagca agcggaagtg cgttaccaac tttgatgtct gcttctggac cagaaataat
    12301 gcgttgacct acttcaaggc ctttaggcgc aaggatgtaa gctttaacac catcagtata
    12361 gtgtacaaga gcaatatttg cagtacgatt tggatcgtat tcaattgttt taacaactgc
    12421 ttcaacacca tctttattac gtttgaagtc aatcaaacgg taatgacgtt tgtgtccacc
    12481 accttggtga cgaacagtaa tacgtccgtt gttgttacgt cctgctttat tctttagtga
    12541 aacaagcaat gatttctcag gagtgtttgt agtgatttct gcaaaatcca aagaagtcat
    12601 attacgacgg ccatttgtcg ttggtttata aactttaata cccacgttat ttcctcctta
    12661 gattattcag cttcagcagc aaacaactcg attgcttttg aatcagctgt aagagtgata
    12721 atagcttttt tagtttttga agtaaaacct gtgtaacgac caacacgttt tgctttgggt
    12781 ttaactgtca cagtgtttac acttgcaact tttactccgt caaatgcagc ttcaacagct
    12841 tgtttgatca aaagtttgtg tgcacgtgtg tcaacttcaa atgtatattt ccctgcttca
    12901 agagcaacca ttgatttctc agtgataaca ggttttttga ttacgtcata caaattcatt
    12961 atgcaagaac cccctcgatt gtagagattg cttctttagt aacaagaagt ttgtctgcgt
    13021 taacgatatc aagaacactt gcagtagttg cagtagcaac tgtaacgttt ggaaggttac
    13081 gcgctgaaag tgcagcaaat tcgttacctt cttcaaggat tacaagtact tttgaatcaa
    13141 tgctaagtgc tgaaagaact gacgcaaatt cagcagtttt tggagctgca aatgaaaggt
    13201 tttctacagc tacaaattta tcttcagcaa cttttgctga ataaactgat ttcaacgcaa
    13261 ggcgacgaac tttttgtgga agtttgtagc cgtatgaacg tggagttggt ccaaagacaa
    13321 caccaccacc acgccattgt ggtgagcgga tagaaccttg acgagcgcgt ccagttcctt
    13381 tttgacgcca tggtttacgt ccaccaccag atactgctga acggttttta accgcgtgag
    13441 taccttggcg aaggctagcg cgttggctga ttacaacatc aaaaacaact gattcgtttg
    13501 gttcgatacc gaagatagct tcgttaagct caactgagct aacttcttta ccagtttggt
    13561 caaatagttt aacgtttgcc attttaactg ttttctcctt tcttattatt tagcagcttt
    13621 aactgctgat ttgatagtga taagagattt cttagcacct ggtacgttac ctttgatgag
    13681 gacaacgttt ttctctggga taacttgtac aatttcaagg ttttgaactg ttacacggtt
    13741 accacccata cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg caacaggtcc
    13801 catagaacct ggacggcgat ggtaacgaga accgtgagcc ataggaccac gtgattgacc
    13861 atggcgtttg ataacacctt ggaaaccttt accttttgat gtccctgtga cgtcaacaac
    13921 atcaccagct tcaaattgtt ctacagataa ttctgcacca acttctaagc cttcaatgtt
    13981 tttgaattca cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat ggcctttggc
    14041 aggtttgtta ctcaatactt cacgtttgtc atcaaaacca acctgaactg cttcgtaacc
    14101 gtctgtttca acagttttaa cttgaagcac aacgtttgga gttgcttcaa tgacagtaac
    14161 agggatgaat tcgcctgatt cagtgaagat ttgagtcatt cccacttttt tccctaagat
    14221 tccttttgtc atgagaaaat atttcctttt cttattttat ttcaaaaagt tttttacgag
    14281 cgttttttgt gctcagaaat caaattaaag tttgatttct acgttgacac cacttggaag
    14341 atcaagtttc atcaaagcat caacagtttt ttgtgttggg ttgatgatgt ctaccaaacg
    14401 tttgtgtgta cgcatttcaa actgttcgcg agaatcttta tatttgtgag tcgcacgaat
    14461 aattgtgtaa agactgcgtt cagttggaag cggaactggt ccagcaactg tagcacctgt
    14521 acgtgttgca gtttcaacga ttttttctgc cgctgtatca agtgtacggt gttcgtacgc
    14581 tttcaaacgg atacggattt ttttgtttgc catcttttct cctctcgtct atttacgata
    14641 ataggctagc tcctcaagaa aaccaacacg agttgcgtgg caatgcaacc tagcgtgtcg
    14701 caacctcttg catcatagct aaagctgttc atacacagca ccagtatatg atatcaaaaa
    14761 aaaacagcac acgcaagcat tattttattt ttttttcaaa aaaaatgcaa ccatttctgg
    14821 ttacattttt tgaatatatc gatgatagcg aactttcaaa aacagccacc taaagatatt
    14881 atctgctatc gtagccatcc aaaccccaag caaacctaat tcaaaaacta ttcctaataa
    14941 ataaccaaga actactctga ttaaccacat gcctatagtc gttgtgtaaa atgggagttt
    15001 agcatttcct aatccttgcc aagctgctgt ataaaccaag gtcccaatag tagcaggtac
    15061 tcctacgaaa gatagcaaaa taacaatcaa acttccttta atggcagaag gattatttgt
    15121 aaataactgt gtcaatggtt tgccggcaag aagcataaaa cttgatataa ggatcattag
    15181 aactaagcca atataatacg aactttgaat agtttcttct atagattctc tatttttttg
    15241 tcctacatat tttgcagtta ggataattgt agcagttgcg atgccaagtc ctggcatata
    15301 attaaattgt gtcaaggttt ctccaatagc atttcccgct acaacatttg tacctaactg
    15361 aacaacaata gcaactataa caacatctcc agctcgcatc atcagacgtt ctcctgctga
    15421 tggtaaggca agattccaaa tttgtgcact gattttccaa gtcattcttt ttattatttt
    15481 tttaatagga agatacttcg caaggataca aataccaatt aatcttgcga taagagttga
    15541 tactgccact ccgaccaaac catagcgcca ctcaaaaata gcaaaacctg ataagactgc
    15601 attgaggaaa ttgacaaaaa tactgacata cattggtaaa cgaggttgcc cttgcacacg
    15661 taaaaatgag cctagggtgg ttaatattcc taaagtaaca acacctccac ccacaattgc
    15721 taaatataaa ccaccaactt gtgcgacact ttttgttgtc cctaaaagtt taaagaaagt
    15781 ttgtccaaaa acaatcgaga taattcctaa tactgcccct atcagtgatg ttagaaaaat
    15841 ggcttgagaa catactgaaa ttgcatcatc cttctcacta cctgctaacg acttggccaa
    15901 tagacttgct atactcgccc ctaaagctat aaaaatagct tgataaatag taattatatt
    15961 attagcaact gaaacacctg atactgctac aacacctaac tgagccacta gataattatc
    16021 aactactccc atgagcattt gtaagatatt ttcaatcata gctggtatag ctaactgaat
    16081 aatctcttta ctataacgca cagtatctcc tttctattac ccaggatcat taaaaagaga
    16141 gtgatgggaa aatttctaaa atgtttcttt ctcgctctaa atattttaaa acaatcgtat
    16201 ttttattatt gtttcaaacc tagacactct tcaaccgttt ctcgcatatt tgaggtaggt
    16261 actaccattc tatggattac ttcggccttt tgtagatctg taaccgcctt aggtaaagaa
    16321 acacctgaca aatcgtttaa gattgaaata gcagcgaaat cctgtatctc ttctttattt
    16381 gtaatagcct ttgtaacaac acacgggaac ttataaggac ttgctgttga cacaattact
    16441 gttggagttt gatctttagt ttctgtctga taagcttgat aaacagctga tgctacagct
    16501 gtatgcggat cctctatata ctgatactgg tcaaataaac gtttgatgtc taattcaaca
    16561 aattgttctg tcgcaaatcc cgctacaaag ctctctaaaa tatttgcttg cctagcctct
    16621 aaggcatact ccccagtagt cactaaatct tccattaatt ttttagtcgt ttctgcatca
    16681 tcgcctaaaa ggtgaaagat taatcgttct agattagagg agaccaaaat atccatagag
    16741 ggacttgatg tcactttaaa ttctcgattt ttatcgtagg tttgtgtctt aaaaaaatct
    16801 gttagaacgt tattatcatt tgaggcacaa atgagtttgg taacaggtaa tccaatttga
    16861 ctagcataat atgctgctaa aatattacca aaatttcctg taggtacact aaaattaatt
    16921 agttgaccaa ttgagatttc ctttgactta actaattgag catatgcata tatatagtaa
    16981 acaatttgag gaacaaggcg accgatgttc attgaattgg ctgatgataa ctgcatatgc
    17041 tgctgtgata attttgatcg aagaagggaa ttattgaaca tttctttaac cgaagtctgt
    17101 gcatcatcaa aatttccttc aatcgcaaca acatgggtat tttgcccggc ttgtgttatc
    17161 atctgcaatt cttgaatata actaacacca tgtttaggat aaaatacaat aatctcagtt
    17221 cctggaacat ctgcaaaacc tgccatcgct gccttaccag tgtcacctga tgttgctgtt
    17281 aaaataacga ttttatcatc aaccccttgt tttttggctg ctgttgttag caaataagga
    17341 agaatggata aagccatatc tttaaatgca attgtaggac cgtgaaataa ttcaaggtga
    17401 taccggtctc caatctttac tattggggct atttcagttg tatcaaattt agtatcataa
    17461 gcttgactaa tacagtaatc caattcttgt tcagtaaaat catcaaaaaa agctgacaaa
    17521 acgagtttag ccacttcttg gtaggaagca tccttaagtt tagtgaaatc taaatcaact
    17581 tttggaaatg tgatgggggt aaagagtcct ccatcgtcag ctaatccctt tagaatcgct
    17641 tgactagctg tcactttatt actagcatca cgtgttgatt gatatactat tgccataaga
    17701 atccctcttt atataaatat cttttagtta ttgtaacaca atttaataac atgtccattg
    17761 ttcgttatat aattaacccc cctttattaa aataaagggg ggcgtaatat cattatcact
    17821 ttgtaaagtc gagaacttta cgtccttgaa tcaatcctct ttccatttca tcgaatactt
    17881 gaggagctgt atccacaggc actttttcaa ccactggtac taccaaacct tctgctccaa
    17941 atgcgaaggc ttcttctaaa tctttacgag ttccaactaa ggaaccaacc acacgaatcc
    18001 catcaagtac tgtttttaca atactcaatt ccatatattc tgatggaaga ccaacagcta
    18061 ccactgtacc tccagcacga acgctgtcaa tcgcctgatt aaacgcaact ttagaaacag
    18121 cagtcacaac cactccatga caaccgcctg tcttttcttg aatataggcc gcaacatctt
    18181 ttatttcttt gccattaact gtcaaatctg ctccaacctc tttagctaat tgaagtttat
    18241 ctgcgttaat atcaacagct acaacatgag cattgaatac tttttttgca tattggactg
    18301 ctaagtttcc aagaccacct gcaccataca ctgcaatcca ctgaccagga gcagctccag
    18361 cttctttgat agccttgtat gttgttactc cagcacaagt gattgatgat gcttgagctg
    18421 gatctaatcc ctcaggaacc ttaaccgcat agtccgcggt aacgatagcg tattcactca
    18481 taccaccatc aacactgtat ccagcatttt taacactacg acaaagcgtc tcacgtcctg
    18541 tagtacagta ttcgcaatga ccgcaccctt caaagaacca tgcaatagag acacgatcac
    18601 caaccctcaa agacgttacg ccttctccaa tttcttctac tataccaatg ccttcgtgac
    18661 ccaaaatgcg tcctggtacc tggccgaaat ctccatgtgc aacgtgtaaa tcagtatggc
    18721 acacaccaca gtattctact tttacaagtg cttcaccatg accaactttt ggaagatcat
    18781 gctcaacaac ttcaacacct gtactagctt gattaacaac aaccgctttc atgataaaac
    18841 ctcctagaaa taaatatatc ttatttgtaa tcgtttacat ttttatattg tattattatt
    18901 cacaaactat gtcaagtgat tttcagaaaa ttttttgaaa atattattaa ctggttaatc
    18961 tttttgaaac taaaaatccc actcaataga gtgagattct tcgactgttg ttacttacgg
    19021 cgtcctggac gttcctcata accataataa gcatcttcga tgatttcttg catgtgatct
    19081 accattggaa ggcgtggatt tgctggtgaa cattgatctt catatgctaa gaaagctagt
    19141 tcacgagatt tttctttcca ttctttttca tcaatgcctt ggtcacggaa attcatctta
    19201 atacctaaac gtgttccaag gtcatacaca gcttttgcat atgattcgac agcttcttct
    19261 ggtgttgctg ctggtaagcc gagtaattta gcgatatctt gatacttttc atcagcacga
    19321 tagtaattgt acttaggcca tgttgctgtt ttagctggac gagttccatt atagcggata
    19381 acatacggca aaagaattgc atttgtacgt ccgtgtacgg tatggaattg tgcgccaatt
    19441 ttatgcgcca ttgagtgaga aatacctaag aatgcattgg cgaaagccat acctgccata
    19501 gttgacgcat tgtgcatctt ttcacgtgct tcaaaatcag catcttttac tgagcgttct
    19561 aagtagtcaa atacaatttt aattgcttga agagcaagtc cgtctgtata atcatttgcc
    19621 atctgtgaaa cataagcctc tgttgcatgt gtcaatacat ccatacccgt atcagccgca
    19681 ataaaaccag gaacagtcat aacaagggcc ggatcaacga tagctacagt tggtgttaat
    19741 gaataatcag cgattggata tttacgatta tttgctttgt cagagataac ggcaaatggt
    19801 gtcacttcag aacctgttcc agaagttgtt ggaatagcaa caaattttgt tttcttacca
    19861 agttctggga atttgaacgc acgtttacgg atatccatga atttttgaac gaggtcatgg
    19921 aaatcaactt ctggttgttc atagaagagc cacataactt tcgcagcatc cataggagaa
    19981 ccaccaccta atgcaataat agtgtctggt ttgaaggtac gcattaaatc agtccctttc
    20041 attactgttg taatgtctgg atctggttct acttcagcaa aaatctgata aacaacttta
    20101 ttgcgacgaa gatcaagttg ttcaatgata cgatccaaga aaccaagctc aaccatggcg
    20161 tgatctgtaa caatcatgac acgttcaaca tcgcgacatt tttgcaaata ttgaatagaa
    20221 tcacgttcga agtatgtttt tgaagggact ttaaaccatt gcatattatt tctacgtctt
    20281 cctacttttt tgatattcaa gagatttata gcactaacgt tatcaccaac tgagttacgt
    20341 ccatacgaac cacaaccaag agtcaatgat ggcaagaaag cattgtaaac atccccaata
    20401 ccaccaaaag tagaaggaga attccaaata acacggatag cacgaattct agttccaaat
    20461 tcacgagcta agtctgcatc ttttgtatga atagcagctg agtgccctag accattaaac
    20521 tcaaccattt gacgagcttt ttcaacacca tcttctgtag attcagcttt caatactgca
    20581 ataacaggtg atagtttttc acgtgtcaaa ggttcttttt cactaacttc agtacattct
    20641 gcggcaagaa tatttgttcc ttctggaaca gtaaatccag cttgttcagc aatccaaact
    20701 gctgattttc caacaatatt tggatttaat tttgcaccag cacagttttt gctatttgct
    20761 tttgcaccaa aacagaattc ttcaagaaga gctttttctt tcttattaac gaaataggta
    20821 tgataagatt taaattcttc aacaaattct ttataaattt ctttatcaat aataacagcc
    20881 tgttctgacg cacataccat accgttatca aatgatttag acataacaat atcgtgagca
    20941 gcttgacgga tattagctga tttttcaaca taagctggaa cattcccagc acctacacca
    21001 agggcaggtt tcccacatga gtaggcagct ttaaccatcg cattaccacc tgtcgcaagg
    21061 atagtggcaa taccatcatg tttcatcaaa gcattagttg catcaatcga tggttgttca
    21121 atccattgga cacaattttc aggagctcca gcagcgatag cagcatcacg aacaatacgt
    21181 gctgcatgtg ctgatgattc ttgtgctgat gggtggaagg caaaaataat tggattacgt
    21241 gttttaagag agattaatga cttgaaaata gcagttgaag tcgggttggt tgttggcgta
    21301 ataccacaga caactcccac tggttcagca attaatgtca agcctgtgac atcgtcttct
    21361 tcaataactc ccactgtctt tgtatgacgc atgttgttaa cgacgtgttc acatgcaaat
    21421 aaattttttg tcgctttatc ttcaaagaca ccacgaccag tttcttctac tgcatgaaga
    21481 gcaagttctc catgggcatc caaggctgca acagaagctt tagctacaat ataatcaact
    21541 tgttcttgat taagttgacg catctcatca agtgctttta aaccgttttg aactaatgta
    21601 tcaattgcaa gagcaacatc tgtagtttct accgctttag ttttttcagt catttgagac
    21661 tccttttgat ttgttaaatg tttcacaaat ttgtttacga gattattata gcatatttaa
    21721 aaactttgta aatagtttca caactttttt gtgaactttt tttcaatctt tcggaaacat
    21781 ttcttgaaag cgctttaatt aaatttgttt tttcattact aattttcaga ttaaagccaa
    21841 tcgatttcat ttgtatcacc ataaacggag gtatctatta ttcgttttca caaaaaagcc
    21901 gagtacatga ctcggacttg tttattttgc agctcgttga gctgattttt gaatagcttt
    21961 tttgacgtta ctaacatata aatcagcccc tgtagtatcg ctatcactat aatgcacacc
    22021 atctgtattt ccccagattt ctggatgttc tgtcgcaacc ttataccagt cagcaaccgt
    22081 tatgtaatta tattttttca ttagcgagag ttcataatca cgcactgtcg taacttgact
    22141 catattttta gcattataag gcgttactat aatcaaacgg tgtcccttag gcaaagattt
    22201 aataaattgg ctcaagtctg tcttgtagtt atctaatgaa ttcacaccaa ctgccaaaac
    22261 aacaattttg gacaaggctt tattttgaat acggttttca aaaatatcaa atgatttact
    22321 aaaattacga ctcaccgcag catctaattg tacttctggt aacaatttat taaaagcagc
    22381 acttgacctt aaggcaactg aatctccgat taccgtcaca tcactaagag cacctgcatc
    22441 ccctgcggct agtgtatgtg ttcggttagt atttgttcta gcttgttgta acgaattctg
    22501 caataattct gtctcaaaac ttcctatact tggagctgtc aacattgtca ctacagtaat
    22561 aagagttagt acccctccga tactaaataa ccactttttg taaggcaaca agtcaaattc
    22621 ataatcaagg aatttaggtt ttctacctaa aataaaaggt tcaatgatat aaaatgataa
    22681 tgttgagaag actgctgata atactactgt caaaataaca gctatccagt ttggggataa
    22741 acgactaaaa ataatataaa atggccaatg aaaaaggtac ataccgtaac tgatatcagc
    22801 aagataagta atcacgaaag gttctgaaat atctggtgta tgctcgtgta aaatacgtgc
    22861 attgtaaatc attacactag caaaaatgct agacaaaaca aaaccaaaaa gatatgttag
    22921 ccgattatca aaatcaagtg caaaagttaa aatcattaga ccagcaaaag cactacccat
    22981 catgattaaa ttatgcttca aagtcagatt tttgatattt ttcttgaatc gtccggtaat
    23041 ctctcgaata ccagatattg tagccaccat cgctcctaaa aagaacggaa atatatgtga
    23101 taatgtagag aaatatatcg ttgaaaaatt atcaacgaaa aaggctctaa caaacatggt
    23161 aaggaagctg actccaaaaa ctcccatgga aacaaggaac aaagtccccc tcaattgctt
    23221 ttgatctttt gatcttttcg ataagagcca taccgttaag ccccataaaa cataaaaatg
    23281 gacttcaata gaaagactcc aagtatgcac aaataagtga gggataaatt gactttcgta
    23341 gttaccacca gtcaagattt cgtaaaagtt gctggtaaaa cctaaagcag tcataatttg
    23401 agagccaata cttgctctaa aatcgctttt aaccagaaat acaaaaggta ttgtcaccaa
    23461 aaccatcaat actaatggtg ggaaaatacg gtaaaaacgt ctacgacaaa aactaacaaa
    23521 atcaattttt ttcgttttag aaaattcatc aatcaacaag gccgtaatca gaaaaccaga
    23581 aaatgtaaag aagatgtcaa caccaacaaa tcctcctgga aaactattct taaagaaatg
    23641 gtaaagtaaa acaagtagta gtcctgttat acgtaccaaa gaaaaccatt ttattctcat
    23701 ttttgataaa tcccctgttt aaatgtcccc ttatagactt tcttattttt agcattgagg
    23761 cgaccttgtc catcaggttg acctttttta aagtcacctg tataagacca gccatgaact
    23821 gaaacaaatg ttcctttccc ttcaaagacc ccatttacaa aatcgccctt atagtaatcc
    23881 ccattctcat aggttaattt acctttacca gtcatatggt gattaacaat agagcccaca
    23941 tattttatct taccgccatc atagctaagg actccttgac tagaagtcgt taaggtaaat
    24001 actgataaac caaaaacaac aataattatc aaagataata tttctagatg agttcgtgtt
    24061 attttaaaat tcttcatttc tacttaatct ctactattat ttattagcgt taaccactgt
    24121 ttacagccat cactgactag acgataggtt tcctcaaaat caccagtata ccatggatca
    24181 ggaacaccac catcacaaaa aaggctaatc ttatcatcat aggttccttg tgatagagct
    24241 tttaaatcct tgacattttg ctcatccata ccgatgatat aatcaaaata tgctaaatcg
    24301 tctttagaaa taggttgtga ccctttatcg ttagtgtaag gaatttgata acgctgcaaa
    24361 atcgcttgcg ttcccctatg tataggattt ccatgctccc atgttgaagt cgcgcgactc
    24421 tcaaccgaga ggctatcctc cttatctaaa ttcgccataa caaactcagc catagggcta
    24481 cggcagatat ttccaagaca aacaaaacag actttcttca tagcaaactc cttttggtct
    24541 attataacag aaaaaaggta cttataatac gtttaactgc tcaaagaata actacaacat
    24601 atctaatact taacattgca tgaaaattaa aaaattaaga ctagataccc tagccttaaa
    24661 atgacttatg ttttatttat caaatcgctg ataacctaaa tgttcgtatg ccttatccgt
    24721 agctacacga ccggtacggg tacgcataat aaaacctttt tgaattaaat aaggttcgta
    24781 catgtcttca acagtatctc gttcttcagt aatattaacg gatagagttc ctaaaccaac
    24841 aggacctcca ttatacattt caatcatggt tcttaagatt ttttgatcga cgtaatcaag
    24901 cccctcgtga tcaacatcta acatcgttaa tgctttatct gtaatattgt catctatcaa
    24961 accatctccc atgatttgag cataatctcg aacacgtttc aataaacggt tagcgatacg
    25021 tggcgttcca cgactgcgac gcgctaattc agaagcagct tcataagtaa ttttcatttc
    25081 aaaaatatct gctgtacgct caataatttc tgtcaaatcg ttttcttcat aatactccat
    25141 atgccctgta ataccaaagc gagcacgtaa gggattagat aacatacctg cacgtgtcgt
    25201 tgcaccaatt aaggtaaagg gcggcaaatc taaatgaaca cttctactag tttctcctgc
    25261 accgatcata atgtcaatat agaagtcttc cattgcacta taaagtacct cttcaaccgc
    25321 catcggcata cgatgaattt catcaataaa aagaacatca cctggttcta aatcatttaa
    25381 aatggctact aaatcccctg atttttcaat tgcgggacct gatgtttgtt tgagattgac
    25441 acccaactca ttagcaatta caaatgccat ggttgttttc cctaaaccag gagggccaaa
    25501 taataacaca tgatccaatg actcatcacg caatttagca gcttcaataa atatttttag
    25561 ctgatcttta accttatctt gtccaatata ctctcttaaa tactgcggac gaagtgtacg
    25621 ttctaccaat tcttcgtcac ccattgcatc actatctaaa aatcttgtca tgatattatt
    25681 ataacatttg tataggaaaa gttaagaact aagttggtgt attagtatct cccttattta
    25741 aaggaagaat aaactgatct tatagttaat ccaacctcaa gaaatcacag aacagcttac
    25801 ttagcccata ttccaccaac ccattcctaa aaacaaatca aatgaaccta aatttgataa
    25861 tagaacaagt aatactatta aaaaacattt tttaatcact ataataaact cctaaataat
    25921 attttgtacg tttattataa atttttagac ctcagagtca caggacatca atgtcctgtt
    25981 atatcttctt gccactccaa ttctaatttt ttaattagat gcttgtcatc aatcattttt
    26041 gaaaacataa ttgattgttg gtaagcattt tctgcagact ttatatcatt gtcaattgta
    26101 atgaagtatt tccattggta cataaaatat aaaggcattt tatgaaaatc atgtgttgac
    26161 accatcgttt gtcggctaac ttctaacatt tctgcaagac actcttcact atttaaacaa
    26221 agtccaacaa atacacaatt caggagtaca tgattaaacc aaaacaaatc ttctgctgta
    26281 agtttgtggt tctgacttat caaagtttta catatagttt gaaatacttc tttagtaaat
    26341 gctctattgt taaatttaga tacaactgca caagttaaaa acaaatctat gataatcaaa
    26401 tcattaagag tatatttttc tttatatttt actttatcaa agcattcgca taaaacctcc
    26461 acaccaaaat taatgtctcc tgtttgatag acttcaaatt tagcttgtaa actgtcaatt
    26521 gctaagcact cgtcttctgg aagattatca tagaattctt cgaaaatatg atcaaactga
    26581 cattctctta attttaatcg ttcagcatct gcataagttg gaattcttaa aatcaaatac
    26641 ttcaactctt tataccgttt aggtaattct aagctctctc ctcctgttaa aatatctaat
    26701 tttacattca attgtttagc tatgtagtga gctttagtca agtttgggat tgactgtcca
    26761 ctttcaatcc tagctaattg tcttacagat aactcagttt catcaccaca aaaatcttcg
    26821 cgagtaatat ttctagttac tctcaaattt cttactttac taccaaaatg ttttaacatt
    26881 ttcctttaaa aactaactcc taaatttttc ttcttattat atattatcat aataaactca
    26941 ttttaagtat gtttttttaa tattaattcc ttttaaatca ataaaaattc cattactggt
    27001 taaaaaaacg ttaaccagta atggaatttt tttatttttc taatcctagt ctttcaaaaa
    27061 tatcatcaac acgttttgtg taataaatcg gattaaatag ttcatcaatt tcttcctgtg
    27121 taagacaaga ggtaactttg gtgtcttctt ctaaaagtgg tttgaaatcc acttgattgt
    27181 cccaggaata agctgtctta ggttgaacta agtcataagc ttcttctcgt gtcattcctt
    27241 tttcaatcaa tttgagcata acacgttgac tataaatcaa accaaaagtt gattccatat
    27301 tgcgcatcat attttccggg aagacagtca agttcttaac gatattgcca aaacggttga
    27361 gcatgtagtc aatcaagata gttgtgtctg gtgtgatgat acgctcagct gatgagtgcg
    27421 aaatgtcacg ctcatgccaa agtgacacgt tctcataagc tgtcaccatg tgaccacgaa
    27481 tcacgcgcgc tagccctgtc atgttctctg aaccgattgg gttgcgtttg tgaggcatag
    27541 cagagctacc tttctgacct ttggcaaaga attcttcaac ttcacgttgt tctgattttt
    27601 gcagaccacg aatctctgtc gccatacgtt cgatagatgt tgcgatgctt gcaagtactg
    27661 caaaatattc tgcgtggagg tcacgtggaa gaacctgtgt tgaaatttct tgcggacgga
    27721 tgcccaattt gtcacagaca tatttttcca caaatggagg aatgttggca aagttaccaa
    27781 cggcacctga aatcttacca gcttccacac ctgcggcagc atgttcaaaa cgctcaatat
    27841 tacgtttcat ctcgctgtac caagtcgcca acttgagacc gaaagtcgtt ggctcggcgt
    27901 gaacaccgtg ggtacgaccc atcatgatgg tgaacttgtg ctccttagcc ttgtcagcta
    27961 cgatgtttgt gaaattctca agatcacgac ggataatgtc gttagcctgc ttgtagaggt
    28021 aaccgtaggc agtgtccacc acgtcagtcg acgtcaaacc gtagtgcacc cacttgcgct
    28081 cctcaccaag cgtctcagaa accgcacgag tgaaagccac cacatcgtga cgcgtgtcct
    28141 gctcaatctc aagaatgcgg tcaatgtcaa aatccgcctt ctcgcgaatc ttagccacat
    28201 cctctttagg aatctcaccc aactcagccc atgcctcgtc agccaaaatc tcgacctcca
    28261 accaagcacg gtatttattt tcctctgtcc aaattgccgc catctcaggg cgtgaataac
    28321 gtgaaatcat tgtttgtctc ctttaactta tttcattaaa atactagaaa tgacattgcc
    28381 taacacccca gttatggtat tctgaacata atcatttttg aagaaactag acattttatt
    28441 gacaaaattt ttcaattttc tttcatcctc tgcctcacta atttccaaca attctgaata
    28501 tatcgcttta tccgcagtag atagctgagt gttaataatt aaatttaact gctcaaccat
    28561 atcagtccat ggaatttcca cacttttatg tatatcggca ttattaatat tagaatcttt
    28621 tatgatagta gaaccatttg atatatcccc agataaacta acttgactac cgttatcaat
    28681 atttgttcca actatattac cactacctga gacatcattt cgactagatc cagattcata
    28741 attatcgccc tttatatttt ggttttttat atcaattttt atattttgat ttttgttatt
    28801 gaacgatttt gtaattacat cgaaaacttt atctctaaaa ttcgaatctt taaaataaag
    28861 aaaatagtcc ctgctagagc ccaagttaaa aataatataa tattttgaat ttaaatgttg
    28921 cttatgcttt ttataaacta ggtaagcata gcctaccaaa aatataagtc ctataaaagg
    28981 aggaatcagt cctataataa ataatacaaa taaaatcaca taatctagca acgttattgg
    29041 agatgaaaaa ttacttctac ttaccattga gatagacgtt atctgaatac tgctattttt
    29101 tactattatt gcattatctt tgattgctaa tgtatctgtc tctaatgtat tagaatcttc
    29161 tctacttaca ctttttagaa tatcttgaat caattcttat ttctcctttt attttttgaa
    29221 attaattttt ggaatgaaag atgcctacaa ataagtcaca attatctttt caacaaagct
    29281 acaactatat tctgttatat acaaatacag tcttttctat ataattattt tgtcatcaga
    29341 ctttgtctat attcaaatgc ttctgctttt tcagtcaact cgatagcaag ttctttatgc
    29401 tcctctaaca ctgctttgat gtcagaaaat gggactttaa aatactcttt tcgcccatta
    29461 actttattaa ttttattttt ttcaaatctg ttatgtaact ctttttctaa tgaaaaagct
    29521 tcctcactaa aaattaaggc atgtacatca aattgaaatg gaacagatgc actccctaat
    29581 tcgcgaatac gttccatagg atctagacgc cttgtaacac caatcttgta aacatcttct
    29641 ccaaatgcac caatatttga aatgatataa acatatccag cagttgcatg cccttctcga
    29701 taatcaatat cttcttctaa ctcatctagt tctgataatt tatcttggta ttctgctaat
    29761 tgaagattta atttttcctt ttcttcctca ttagctgttt taagtaattc ttcaacgtta
    29821 tctaacatct gtttaaagtg ttttctatct ttttcaattt gttttctctt agcagctaat
    29881 tctgcttgaa gtttcttatc ttctctttcc ttcgcacgtt gttcacgaag aagttctttt
    29941 tcttctaatt tctgaagttc atattctact gctaattgta actctgaaat ttttagctca
    30001 agatagtcat tagatagaca taccaaattt cttgaataca tcttattaag ctgattaaat
    30061 gatttaacaa ctgcatctac ttttttactt agattagtag ccgttacctt taccaggtga
    30121 gcatcaactt ctccattaaa acctcttatc atcgctttaa taagttgatt ttgcattgct
    30181 cgtccttttg atttactgtt atccagcaac ataggtctta caataacacc cgctttttta
    30241 tcttttacta actgtttttg cctctttctc agagtttcca attcttcttt atacttagta
    30301 ctatctgaga acttatattt tctctcaaaa aatccgtact cttgaagatc taaatcatca
    30361 ttaagaacta ttacctcttt ttgtaaagct ttaagttctg attggcgttt cttaagttca
    30421 tcatcaagtt ttaaaatatc tttagcaacc tttatctgcc ttgttaaatc atccaaaata
    30481 gattgtttat cttttaactt actttctaaa ctactgattt tagcttccaa agcctcatct
    30541 ttaggctttc cgaatccaaa tagacccatt tattccccct aaaactcctc cacctcatcc
    30601 gcatcttcca caaacaccgt tacatgtccc attttgcggt tatgtttcgc ttctagttta
    30661 ccatacatgt ggaggtgagc gctagggttt tgggcaacat agtcaatcgc ctgctggaca
    30721 tgttgtccaa ggacattaag cataacggct gggtcatgga gcttgattgg tgggagaggt
    30781 tgccccagta cacccaagat gtgggtgtca aactgtgaaa agtcacaggc ttcgatagaa
    30841 tagtgccctg agttgtgggg gcgtggggcg atttcattga cgatgatatc atctgcggtc
    30901 acaaacattt ccacacagag ggttcctgat agctggagtt ccttggcaat ctgtaccgcc
    30961 attttcttag ccttgtcagc tagttggtct gagatgcgag ctggtacgat agtttttgat
    31021 aggatattgt tgcggtggat attttcctga acgggaaaga ccgtcacatc cttgccattt
    31081 ccagacacga tgactgagat ttcaaggtca aagtcgacaa actcttccaa gacacactca
    31141 gctgagttgg ctaattgttg cgcctctggc aggtcttctg ctgagcggat aaccttttgc
    31201 ccatgaccgt cataaccacc tgtcgctgtc ttgaggacat aggtcttggt caagtcaagc
    31261 ccctctaggt caaggctaga cgtcaccacc ttatagggag cgacagtcac tccagcctta
    31321 tttgccagaa agtctttttc aaagatacgg ttttgagaaa tgcggagcag atcagtcccc
    31381 tgcggtagct gaccagctga cacaacggca tccagaccat cggcatcaac attttcaaac
    31441 tcataggtca aaacatcgca acgcgcagct aattgtccca aagcctcaac gtcatcgtaa
    31501 ggcgccacaa tcacctcgct aacgcgggag gcagggcagt cgctagcagg atccagcgta
    31561 atgaccttgt ggcccatgta gatagccgca atcgccatca tctgccccag ctgaccacca
    31621 ccgataatcc caatggtctt aaatgagttc attgcttgac tcctctgcga tttttccttg
    31681 ttcctcatgg aaatgagcta gcgcatcagc cagattttgg tcctcgatag agagaatacg
    31741 gagggctgtt agggcagcgt tagtcgctcc cgcttctccg atagccatgg ttgccacagg
    31801 cacaccgcca ggcatctgca caatggaata gagcgaatcc aagcctgaaa gggcacgtga
    31861 tttgacaggc acaccgataa caggcagggt tgttttagct gctaccatac caggcaagtg
    31921 ggctgcccca cctgctccag caatgatgat tttgatacca cgaccacgcg cctcctcagc
    31981 atgcttgaac atgaggtctg gcgtacggtg ggcagagacg actttcttct cgtaagcaat
    32041 gccaaagtta tctagaactt cagccgtttt ttgcatggtt gtccagtcgg atttggaacc
    32101 catgataata gaaataattg gttgcataat attaagatac aaagggacgt ttcgacttgt
    32161 cgaaaatttt cgtagaaaaa tatgcggtaa gtccgaaatc tttgatttcg aagacgcacc
    32221 cacgtcaggg cgactaggtg ctttagcagc agacgcctac gacgcaaaaa tctgacgaag
    32281 atgctttagc atctaggaag atttatcttt ttcactagaa aattagtccc gtgttcagtt
    32341 cctttcttct ttctttttgg agcgaccttc ggtcgctgat tgctgacgct aatatttgaa
    32401 tgagtagtct tattacttaa tagctttgct tccgatgtcg tttcggtaga agaggcctgt
    32461 ggtgtcttgt tgggctagtt gggtatagat tttgtcctgc cctgctttga cgctgtcttc
    32521 tgtggtgaca agcatataga ctcgtccacc gtttgagagc agtgctttgc cattttccgc
    32581 aaacttagct ccggcatagt aggtgatgat gtcgccgtcg gtcttttcag gcagtggcac
    32641 acctttctcg taatcgagcg gatagccttc tgaggcaacg acaacgccca aagtcacgcc
    32701 gtccttctgc caagtgatgt aaggctcgat gcccatcatg atgtcgtcga tgttctgagc
    32761 aaaatcagaa gtcaggcgag ggaggataat ctgagtttct gggtcaccga aacgtgagtt
    32821 gaactcgata accttcggac catcagccgt caggataagc cccgcataga ggacgcctag
    32881 ataaggacgc ccttcagtaa tcataccctc aaggataggc ttaacgatgg tttcaaccgc
    32941 cgtatccact acgctctgag gcaggtgggg aactggcgca taggcaccca taccgccagt
    33001 gtttggtccc atatcaccgt cataggcaca cttgtgatcc tgagctgtcg gcatgatgta
    33061 aaacttgtcg ccattagcga aggcaaagag ggagaactct tcaccatcca aaaattcctc
    33121 gataaccaca cgcgctcctg agtcgccaaa cttgttgtcc aaaagcatct cttgcgccgc
    33181 ctctaccgcc tgctcaacgg tttcagccac gaccacgccc ttgcctaacg ccaagccatc
    33241 agccttgacc acgattggtg caccctgctc ttcgatgtag gctttagctt tttcaaaatc
    33301 tgaatatgtg ccataggctg ctgttggaac gttgtattta accatgattt ccttagcaaa
    33361 gtcttttgac cactctagct ccgctgctgc ctttgttggc ccaaaagctc taagtccagc
    33421 actgttgaaa tcatcaacga taccagccgc tagcgcatca tcaggaccaa taaaagtcca
    33481 agcaatctca ttctccttag caaagtcaat cagcctagaa tgttcggaaa ttacgatatt
    33541 taccaagcct agaccatcca aggtcatacc atcattacca ggtgccacaa aaacctgatc
    33601 tacaccctta gacactaaca acttcttagc aatcgcatgc tcacgaccac cagaaccaac
    33661 aacaagcaat ttcatatcag aataactcct attaaataat ataaaaagaa atttgcagta
    33721 aaaacgtatt atttataaaa ttataacaca tttgttcgct tttaagtttt ttgttgcatc
    33781 ttttttgaaa aattaataac ataatatata cttcacatat attatgttat taatccttca
    33841 tattgctatt taaaacttat cattgctgtt tcttagaact tagtttggcc cctcgctaac
    33901 tttatgttat actgaatatg ttaagcgctt tcttaaagag gagaggaccg tgcattatga
    33961 ctatttatga ccaaattgag tctgctcttg atttaatgac tgatttagaa catgaaattg
    34021 cttgttattt tatggggcag cccatttcaa aagacgctct tgcttcttcc attgtcacta
    34081 agcaactaca catttcacaa gcagccttaa cgcgttttgc taaaaaatgt gggtttaaag
    34141 gctatcgtga atttgttttt gaatatttaa aaagccatga gaccatcagt caacaattat
    34201 acggtcttca aaatgacaac actaagaaag tatttatgaa ttatcaagag atggtttcta
    34261 aatccgctga tattattgat gaagaacagc ttctagaagt atcacatatg attgagcaag
    34321 ctgatcgtgt ttatttttat ggcaaaggga gctcatcgct agtagctaaa gaatttaaaa
    34381 tacgccttat gaggctggga gtgatttgtg aggctctgga tgatacagat agtttctcat
    34441 ggactaatag tattgttaat gatagatgcc tcgtcattgc cttctcactt tctgggaaca
    34501 ccaactctgt tatcggtgcc ttaaaaatcg caagttgtca cggagctaaa accgttctat
    34561 ttacaaaaca acctcacaca attgactatg cttttgacaa gattattcag gtggcctctg
    34621 ctaggcattt agactacggc aatagaattt cgcctcagat cccaatgtta atcatggtag
    34681 atattatcta tgcacaattt ttagatatta ataaaataga aaaagagcga atttttagag
    34741 aaaccattat tcaacgctaa atgaaaatcg agtagaaata atctactcga ttttttgctt
    34801 tgtaaattga cttcctgtca gttgatcaaa aatatagtct tttacttgta ctgtcattgg
    34861 atcatgtcca tagtctggta ataaaagatg ctttttagtt gatgtcaaac ggttaaaaat
    34921 ggcaaattga cttgatggag gacaaatatc atctttcaag gctgttagta aaacaacggg
    34981 gcaagaaatc ctatgtgcaa aatttttaac atcaatataa gccaaagttt ttaacacatt
    35041 attttctgtt ttatgaaaag gatcactata tttaaaatag cggaagagtt cgtcataagg
    35101 ctcactgaca ttccctaaat ctaatactcg tctaaagtct gacaaaaatg ggtaaacagc
    35161 aacggtcttg acgattttgg gattaagagc tgcagctatc aaagcaagtg ctccaccttg
    35221 agaccagcca taactataaa gttgattact atcaacactc tctaatgtag caatgatatc
    35281 aatcaattgg aaaacatcaa gataaatgtc tttataaaaa agatgatttg gtcctgagat
    35341 cattcctcgc acaatctggc ccttaactgt aatgccatca aaatgcccct tgtcttgaga
    35401 ctgtccagct tgacctcgaa catccattga aacaacgccg taacctgctg ctacataatt
    35461 aagttggtca ctccagtcag gactttggtt ttggtaacca tgaaaataaa aaactacagg
    35521 atagggttta tttgttttag ggaataaaca tttggcatag acaatagagt tattgctgcc
    35581 tttaaaatga agatcataag cttgtgcaaa agtaatattg aaatcttttt tgtctagacg
    35641 atactcaata ttcccttgat atttcattgt ttgttttttc cagaagtcat caaaatcctc
    35701 gggaatttgg tcttgaccta ggtattctct catgtcatct agcgacattg tttcaatcat
    35761 atacccgtca ccccctgttt ttaaaatggt agtaagctcc tagcatacca gcttggtttt
    35821 cgtgttgtgc aaaaactatt attgtttttt cagcaagact tgtcacaaga tttcgtttca
    35881 aagattcact aagtttatct tgtaaataat ctttttgagc catgattcca ccacctaaaa
    35941 caactttttc aggatttacc acataaacca tattagcgat tccttgacca aggtagttta
    36001 tcatacggtc tatacttgca atacatttct cattgccttc tttagcctcc tgaaaaatac
    36061 gacgtccatc ccatcggtta atttcatctc catgcgcttt ggcaacatca gcaatcagtg
    36121 cagttgtaga ggctaaatct tgaaaatcac catctgaaag gtgcatatag ccaacttcac
    36181 aagcagaatt actaaagcca tgaaaaactg ttttatcaat aatcagacaa ccaccaatac
    36241 ctgttccaat agtaagacaa agcgctattg aactatcttt ggcactacct gagacagctt
    36301 ccgctaatcc tgcacaatta acatcatttt caatttctgt gcgtacttga taagtgtctt
    36361 ctaaaacttt cttaaattgt gtcccagcat agtttggaat ttgaggacca gagtaaaata
    36421 tgcatccttc atctggatct accatgccag cagatgagat agcaattcca tcaatgctcc
    36481 cttctgttag atagttatcg ataatttgac aaactttttg taaaatgcct ggaccacctt
    36541 tgtaggcttc tgtggcaagc tcacttgcct caacaataca acctaaatta tccacaatgc
    36601 catgcttaat cattgtaccg ccaatatcaa ttgctactgt tctggtcata attatctacc
    36661 tcactttaac aaaactgttt tttagcatgt ctaatcattt ctgctgcctc tttaataata
    36721 ggtttatctt cttctgttac agatgctaaa ggtaaccgaa cggaacctat agttagttgc
    36781 tcattgattt ccaatacagc tttgatcaca gcatacatat taccatgacc cgaacaaagc
    36841 tttgtgataa tatcgttaat tgtaaattga agttcacgcg ctttttctaa atctttatca
    36901 acaattaact gatttaaagt caagtataat tctggcatta cgccataggt accaccaata
    36961 cctgcagctg ctcccatgag acgaccaccc aaaaattgtt catctggacc attaaaaaca
    37021 atatgatttt ctcctccaat agccacaaaa ttctgaatgt cttgaactgg catggaagaa
    37081 tttttaacac caattacttg cggattttgt aacatttttc gatataaatc cgatgtcaaa
    37141 gcaacccctg ccaattgagg aatattgtaa ataataaaat cagtttgtgg tgctgcttga
    37201 ctaatagtat tccaataatc tgctatggca tactctggta atcgaaaata aattggtgga
    37261 attgctgcaa tagcatcaac acccatagct tcagcatgca tagccaactc gacgctatcc
    37321 ttagtattat tacatgctac atgcgctatc actgttagtt tacccttagc aacggacata
    37381 acattttcca gtacaagttt acgatctgct acactttggt aaatacattc tcctgatgaa
    37441 ccattaacat aaagtccttg aacaccttta tcaataaaat aattggtcag ggcttttact
    37501 cgttcgggac aaatatcacc cttatcatca tagcaagcat aaaatgcagg aataattcct
    37561 tgatattttt gtaaatcttt cattgataat ccccttgttg tttgctgaaa taggcaaatt
    37621 gtttttgtaa aataataatt ttaaagtaag caaaggcggt ataagcaaaa ataaatatag
    37681 ctgacactgt aagcataaat aagagactag actgcaccat aataaaccaa gtcatgatta
    37741 ataaaaacat caatacacca gtccatttca agtttaaaca tgccattata aagctctttt
    37801 tcacggtatc taaaagagac atttcatagc gggcagccat aggaaatagg tatatgctcg
    37861 tcatcacagt caataacaaa aaggcgtaac aaattatctt caagaaaggc acaatgaaac
    37921 ctatttgata atgaagaatt gttaaatcaa atatgatcac aaccaaaata cttagttcaa
    37981 ccaaacctaa aagcatacct tggaaccact ctttttttag gtgttttata tacaatatta
    38041 aaagatttgt ctgatcacct tctaattttt gccacaacgt tcgatacaaa ctaattttag
    38101 cagcaccaat agttaccaat ggtaggcaag ttatcataaa gagaaggttt agcaatgcca
    38161 gatcaaaaac aacattgcaa ccttgcataa aggggttgtt aagatcaaaa atcgctgcta
    38221 ttaattgatt tgcttttttc acaatatact ccctgtattt cttacgccat taacactttc
    38281 aatacccctt ttttgacata attacctaga cttcccatac catttggctg atgagcttct
    38341 tctgggaaaa agaaggctat ataattatct ctcaaatcaa aagaagtctc ttctgagcac
    38401 ttgacaaatc ctatatcgcc taagtattcc tctacctcta ccttatcacc ttttcccaat
    38461 ttgataactt catatccttc aatgacataa tgtatatcaa ggtatttttt atggtattca
    38521 aaaatatgat caaatgactc actaagttga tttgattgga ccatgagtat cacttctggg
    38581 ctaatatgat aggtgcctat atctaaattc cttaaatcat gacttagtag ataatcaatc
    38641 gctaaatcta aattaggatt aatatcttta taatgcgtta aattcaataa atgatcgtaa
    38701 atcataataa atcctaccta tcctttaaca gctcccattg taatcccctg agtaaaggat
    38761 ttttggaaaa ctaagaatac tgtcacaata ggaacagcag ctaaggctgc acctgccata
    38821 attaagccat agtttgttgc catttcagcc tgcatagtag ctaccccaag agaaatggtc
    38881 agattatttc tagaagttag cataactaat tgcataaaat agtcattcca agtattgata
    38941 aatgtgaaaa tagctagggc agcaaatcca ggtttgacaa ttggaaatgc tacattaata
    39001 aatgttcgaa tctctccaca accatcaatt ttagctgatt ccagtaattc tgttggaata
    39061 ttttcagaaa actgtttcat caagaagacc ccaaaaggcc aaccaacaag aggtaaaata
    39121 accgcccata aggtatcatg tatccccata aaattgataa ttctaactaa tggcactaaa
    39181 acaacttgtt taggtaaagc catagcagca ataaaaaggg agaacaatat tttttgaccg
    39241 taaaatcgtt tcttagctaa aacatagcct gccattgacg atgtacagca aactaggaac
    39301 attgtcataa ttgagataaa gacactattc caaagccatc tcaaagctgg gttttgtaca
    39361 gttaaagctt taaaattttc taaggtcggt gctttgggcc accattgtgg ggggatgata
    39421 atagtatcag gttgagattt aaaagctcct gtcataatcc aataaaatgg aaagataaaa
    39481 agcactgtca aaagacaaag gataagagcc gttaaaaagt tataagcact gaatgttttc
    39541 tttttcatgt tttcctccta atattccaca tcatttccta agatcttaaa ttgagcaaaa
    39601 cttattaaag caatcattac tgctaggaat actcccatag tattagcata gccatattct
    39661 gatagtttaa aggctttttc ataaagataa tacatcaaag tgcttgttga ataatttggt
    39721 ccaccagatg ttaggagctg gattaaggca aaacattgaa atgaattaat tgttgttata
    39781 actgcaatat agagtgtagt tggtaagaga ctcggccatt taatttgcca aaaaacttgc
    39841 atttcattgg ctccatcaac acgcgcagct tcgcaaagag aattatcaat atttcccatc
    39901 gccgcaatat atagaataat aggttgacca actgaggtcg ttagcaaaat aataataata
    39961 gcaagtaatg cccagtgctt atcaccaagc cagctaatat tctgttcaat gacatgacct
    40021 gatttcaaga tgtaatttaa aattccggac atagggtcat aaatccattt ccaaacaact
    40081 gtgactgcaa cactaccagt tactactggc aagaagaaga cacaacggta gaaagagcgt
    40141 gaaaaaacat ttttttcata agtattggca gcaacaaaaa gtgagaagaa aaccactact
    40201 ggcacagagc caatcacaat gattactgta tttatcaagg atttcatgaa aatagaatca
    40261 tgaaacatac gattgtaatt agctaaccca ataaaagtga actgtgtcat agaataatta
    40321 aaaaaactag tgacaaagcc cataaccatc ggtgcaaaaa caaaaattaa gaaaaataag
    40381 agaataggcg ctagaaaagc ataagctatc atcgtttccc tcatctttaa cttattagta
    40441 cgcatacggt actctccttt acatcttaac ttgactaaaa actcctcttc tatcccaata
    40501 tgtgattgac catagaaaag gagtctcctt aattatttag ctgctttttt aatggtatca
    40561 tttgcttttt gagtaaagtc tttcaaagca tctgctggtt tttcatcacc attggataca
    40621 gattgaacca ttgggaacca taaggttctc atttcagaaa atccatcgat agtgttgtaa
    40681 tatggtgaat aatattgagt ccattttgaa atcttcatca tacgtttatc acctttataa
    40741 agatccccaa atgatgttct aactgggaaa gcacctgtac gtataacatc ttttggtccc
    40801 cattttttat catcagcaat aaaagtgata aatttcttag aggcttttac tttgttttca
    40861 tctttattat taaagaccgc aaaaccatta acaaggtatt ctaaatctgg ttttccatct
    40921 tctgatggga atggcacttc aaggtaatcc actttacttg actctaataa ttttgcttga
    40981 gtttttggtt gagctggagc ccataggata gtgaaagcag tttgtccatt ggcgaagttt
    41041 tgaatgtcag ctgagccatc atactgagac ccattcatca agtagccttt cttaatccat
    41101 tcaactattt ttttcattga ttttacagaa tttttagtgt cagtggtata ttttgttact
    41161 tctttatctg ttattggagc actataaaga ttagcaaaaa atgcacgtgg tccttgatct
    41221 cctccttgcc cgtttgcaaa gaatgaacct ggtgtatagc ctttattttt tagtgctttt
    41281 agtacttttt caaaatcact agtagtccaa ccttctttta caagtttcaa aactcctgca
    41341 tctttaagca tttttttatt gaacgccata taaaatgggg cagaacttat tggatacatg
    41401 taggctttat cgccagactt agaagcttga atgatgttct tattattgac atccttaata
    41461 aattggtctg taaataaatc attcaaatct gctaatttac catttttacc atattgaata
    41521 attcgccctg gtgcatcaaa aagcacatca ggtgctgtcc ctgcttcaat tgctgtagtg
    41581 attttttcag gtccagatgt gaaatcaatt gtctctagtt ttacttttat attaggattt
    41641 ttcttttcaa aagccttgat gacttttttc tcataagtac ctactccatc cttagccttt
    41701 tcttgagtaa aggttggaaa agcccaccac gtaatttcga ctttatcctt ctcggcagcc
    41761 ataacagatt gactactgtc tacacaagca aacattgata atgctgcagc tccgaggcaa
    41821 aaaccaatca cacttttttt gatactcata actattcccc ttttttattt ttattttgat
    41881 ttttgagtaa cacttttaac taagtcctga gatgaaacgc tccgctattt cttttggtct
    41941 agtgatagca cccccaacta caattcctgc aacacctata tgattaattt cattagcctg
    42001 cttaggagta tgaattttac cttccgcaat cacatctata ccggcttgac aaagcttatt
    42061 aaggagttct atatccggtc cttcttcttg gcggctgtaa tctgtgtatc ccgatagagt
    42121 tgtacccaca aaatcaactc ctgcttcaaa agcatttttg ccttcttcaa aagtacttat
    42181 atcagccatt aacaactgtt taggatattt cctttttatc ttttgaataa actcagctac
    42241 actcaaacca tcatgacgct ctctaagtgt acaatctaag gctattactg caatatctaa
    42301 agtagctaat tgatccacct ctgtcatcgt agcagtgata aatggttctt gtggaggata
    42361 ttcacgttta ataatgccga tgataggtaa attagtaact tcttgaattt ccttaatgtc
    42421 gcggacacta ttggctctta taccaaccgc tcctgcttct tgagctgcca aagctaaaag
    42481 aggcataaca cctccacttt cagtataaag aggctcccca ggcaaagctt gacatgacac
    42541 aataatgcca ttttttattt gctttttaaa agcttcttta cttagatgtg gcatattctc
    42601 acttccttat cttttttaat tttagatatc tacaaacctt attatatcta tttttaaagc
    42661 gttttcaata atctttacag acttagattt tagttttaac gttttgattt agtcacggtt
    42721 aacgtgaaag tagtttcaga cttatagctt aatctcctca atttaataaa aattatataa
    42781 gaaccttgaa agtctattca aggcatataa aaaagaagcc aaatagcttc cttttttata
    42841 ttatttgtta aatgatacgt gaacgtggtc atagtggttg gcagtaacgc caccacgatc
    42901 tggcattgca ttccaagtat tagcaggtcc ataaatacta tttgtatttg aataaaactt
    42961 ttgttgccag ataacatatg aaatgttatt tgctgccata ttttgtgtag agtactgtgc
    43021 aacttcatta ccaagtgctt ggtttttacc tacaataaag tcaactgcta aacctttacc
    43081 atgatcacct ggatctcccg cacggtatgt actgaattca ttaactccat aagttgacgc
    43141 tactttttct ttataagctg caacatgagg ttggagccct gcattttcag gatgtgcagc
    43201 tactgcattt gttgttgaag ctggttgtgc taccggtgtt gctgttggag ctttttgtgc
    43261 taccggaacg ctcttaactt cagtcgcttg taacttactg tctgtagctg gtgaagtcgt
    43321 agtcacagga actgctggag ctgatacatg ctctggtgat gcaccagttt ctactttagg
    43381 agtgactact ttagcacttg ccactctagg ggctgctaca gttcttaccg gtgctacttt
    43441 agctactgga gctggtgttt cagcggcaac agaagctggt gatactgttg ttaactgact
    43501 gactgacgtc tgagttggtt taacttcctc tttagctgct ggaacttctg aagtaatcga
    43561 cttcacagga gctggtgata cctgttcatt agctgctact tgactaacag cttgctcttg
    43621 tgctaatact tcttttgatt tcaaagctgg cgcagaagaa tatgtcttca ttggcgaaac
    43681 aatcgttgtt gctgcttctg gtgtcatacc ttccgaaatt gtattgagag aaactttttg
    43741 gtctgcaaca aaaacttgat tggttttcaa atcgacagta gctgttgttt gaccagcagc
    43801 atttgttgct ggtgtttcta ttttcattga agtggcagta tgactcttct gatcgtaagt
    43861 tactgtcagt gttgtctcag gataaataag attgatatct gcaatgttat taatttttgc
    43921 taagacattc atatcaattg acattgcttc tgaaataacg cttagtgtat caccatattt
    43981 cacagtatat gatgatttat tgtcttgctt taccaaatca gcctttacct ctgaaacagt
    44041 acgtgctgtc cacgtcgtat ctgtttcttg tgcttgaaca cttgcgactg ataatagcga
    44101 agctgccatt gtcgatgtca atagtacctt tttattcatt ttcattgctt caattcctat
    44161 ttccttttta ttagtccgag actataatat cacactttac tggactataa aattattttc
    44221 agacggttta aagaaattgt acttagttag taacaaaact gtcatttaaa acaatgaatc
    44281 aatacctcaa actatttgta aagcagttct tgactgttgt caacgcgaag ccaaactgaa
    44341 ctaccattag caaagcgtgt cttagcccaa gttaaaccag ctgttccttg gatgggtttt
    44401 tctacagtct taagagtttt aggattaaag ataaagtaac cacctttttg aagaacctgg
    44461 tcagctgtca aattaccatt agcatcaacc tcatcaattt ctgatgctgg tataccatta
    44521 tcgttccaat caaacccagt cggcgttagg gtgttatttt tgactaacca aacaccatta
    44581 accttttgta attcatctac tcgatacact tttaatttac tagattgatt ctgtacagga
    44641 gctgattgta atggcttaat gcttggagct gatgcttgcg tttttccact aaaggtcgca
    44701 acgttagcaa ttagtgacgt tggattgata cgtccatgga aaccattttg aaaattaggg
    44761 ttagctggta aaaattcaaa atgaaggtga ggtcccgtcg ccataccagt tgctcctacg
    44821 taaccgatga tatctccttg tttgactttt tccccagtcc tagccaccac acgtgacata
    44881 tgagcgtaac cactatgcat tccatccgca tgttgaatca tgacacaatt tcctgctaag
    44941 tctgtcatcc aagaaaagtt ggctccagct cctgcaaatt tcacagtacc atctgccact
    45001 gccctaataa tcgttccagt cggaacagca taatccaccc cacaatgtcc aggataacca
    45061 ttgaaacctg ttgtaattct accattatca attggacgga cataagtatc cgctaaaact
    45121 cgggaacccg cagataaaac cataccacct aaaacaacta aggaacttgc cttaactaac
    45181 catttattca agaaaaacct ccaaatagaa ttaatataat gaagtgctca aacacttgca
    45241 attaacttat tcgaaaattt aaatcaaaat atcaaaaaaa gtactcacct tgatgagtac
    45301 ttaggaggtt taataaaaag aaaagttttt aggatatacg tagtatacct gagttaactt
    45361 aagaaaacct aaactactat tttttaatgt ctaaaatgtc tcacgcctgt gaagatcatg
    45421 gtcaagccat gtttgtttgc ggcgtcaata gattcttgat cacgaactga accacctggc
    45481 tggatgattg ctttgatgcc tgcggcagct atttcttcaa tgttgtccgc aaatgggaag
    45541 aaggcatctg atgctagaac ggcaccgtca aggtggtcct tagcctgctc gatagcaatc
    45601 ttgactgagc cgacacggtt ggtttgacct acacccagtc caagcgtcat gtgatcgtta
    45661 gtaataataa tcccgttaga cttaacatac ttgatagcct tccaggcaaa ctcaagggca
    45721 gtcacctctt gttctgttgg ctggcggtct gtcaccactt gccagtcaga tggattttca
    45781 gccacaacgt cttggttttg caccaaaagt ccaccaacta cgccagtgta ctcagcttcc
    45841 acttcgctgg cagcttgggc atcaaacggc aactcaagga tacgcaagtt tttcttttta
    45901 tttgtgagaa tagctagcgc ttcttctgag tatgatggtg cgatgatgat ttccaagaag
    45961 atagggtgca tcttctcagc tgtcgctgcg tcaacttcac ggttaaggac aacaattcca
    46021 ccaaagattg aaactggatc agcttcataa gcgtaatccc aagctgtctc aatatcatca
    46081 gcctgtccga taccacatgg gttcatgtgt ttgagggcaa caaccgttgg actgtctttg
    46141 aaatcgcgga taatacggat tgctgcatca gcatcacgga tattattgaa ggacaattct
    46201 tttccattga gctgtttagc tgaagcgatt gagtagtctg ttggtaaggc tttttggtag
    46261 aaatcagcgt cttgctgtgg attttctccg taacgcatag cctgtttaag atcataagtg
    46321 atggtcaatt tttcaggctt agcctctccc acttgagctg tgaagtactc agcaatcaaa
    46381 gcgtcgtagg ctgccgtatg acggaaggcc ttagctgcca agcgttgacg agttttaaat
    46441 gtcgtctgac tagcgtcagc caattctccc aaaacagtgg catagtcagc tggatcaacc
    46501 acaacggtta cgctagcgtg gtttttagca gctgagcgaa gcattgatgg accgccgatg
    46561 tcgatgtttt ccaccgccaa atcgttggtc acgtctgggc gaaggatagt ctccttgaag
    46621 ggatagaggt tgacaaccac gaggtcaatc aactcgatgt tgttgtcctt agcagcctga
    46681 aggtggctgt cagcgtcgcg acgagccaaa atcccaccgt gaatgtttga gtggagggtc
    46741 ttaacacgac catccatcat ttctggggag ccagttacat cgtcaatagc aatggtctca
    46801 acaccagcat cgtcaagggc aaccttagtc ccaccagttg agataatatc ccaacccaag
    46861 tttttcaatt cttttgcaaa gtcaataatt cctgatttgt cagaaactga gattaaagca
    46921 cgtttagtca tgagttctcc ttttcttact tcaatctatt tcataggcta tgtattcatg
    46981 agaaaattca taaccgagtt tttcagataa attcaatgaa gtccttgtat gagcatccca
    47041 gctaggataa attcccttat ctaaacaagt tagtatcaac tgagctgcaa ctattgttgc
    47101 taaaccacgc cgacgaaaat ctggatgcgt atctacttct atctcgatcc catttttata
    47161 ggttgaataa gatgaagctc ctgcaatgat attcccctga taatatataa cataacctat
    47221 accttgtttt ttatagtact gataagtagc gtaatttgct actaaatcct gtgaccattc
    47281 cttttctaag caagagttgt aaactttctc atcaataaca cgtaattcaa aaccatttgg
    47341 caactgagta acaaacttct ctaacctact tcgctcaaac aaagtatctt ttttcgtcgc
    47401 ataacgctta aaagaatggg cattctggcc ataagttgat tcaatcaaat ctgaccatcc
    47461 tttatgctga ggaacaagga taatatcctc cccagagcaa acttctagta gaaataaagt
    47521 aggctgacct gctagaaagc caaaagatga tttccttccc aattttgcca aagaagattt
    47581 tggttggtct aaactatcta caaaaacttc tcccataata ccttggacac acgaccagat
    47641 tatggtctcg tcccaatcac caaaaataga ctctactatc tctaaatttt tcactagttt
    47701 cattagctta ttataacaaa ttttactatt ttctctttat gcccaaacta tccaaaacat
    47761 ctggatagag ttgatattcg gtttcatgga tgcgggtttc aaagctttct aggctatcgt
    47821 ctgctaggcg tggcacgcgc acttgttgga tgacctgacc ggtatccaca ccagagtcca
    47881 cccaatggat ggtcacacca gactggtcaa cacctgcctc ccaagcatcc ttgataccgt
    47941 gagcacctgg aaattcaggc agataggctg ggtgaatatt gatgatacgc ccttcatagg
    48001 ctgagagcaa ggtttctccg acaatcttca tgtagcccgc cagacagacc aagtcaatct
    48061 cgtgtttgtc caacaaatca acgacggctt gctcgtaagc cgccttattc tcaaactctt
    48121 tgagttcaaa agcgaagctt ggaatggcta agttctgagc acgctctaaa acataggcat
    48181 cacgatgatc tgaaaagaca aaactaactg gaaactgctc tgctatgacc tgaaagttgg
    48241 aaccattagc agaagcaaaa acagcgattt tcatattcga tacaaaggga tgattcggct
    48301 tgccgaaaaa tttctagaaa aataggaaaa cgaacaacga cgcttgcgtc taagtcgttt
    48361 tatctttttt cccagaaatt taatcccgtg ttcagttagg caaactaact aaacactcct
    48421 ttcttataaa atttagtgac ttatttaatc accacactat cgtctgcttt cttgatgata
    48481 cgaccgattt cataaactgg ttcgtccaag agctctttga cgcggttaac attttcagga
    48541 ctaacagcta gcataagacc gacacccata ttgaagattt caaacatttc ttcgtgcttg
    48601 atgtcaccat atttttcaag cgccttgaaa atcggaagta ctggcacctt atcctcatcg
    48661 atttccgcag ccaaatcatc cgcaaacata cgaggaacat tctcgataaa gccaccaccc
    48721 gtgatgtggc cgataccgtt gactagttct tccttgatta atggcagagc tgcttgaaca
    48781 tagatacgag ttggctcaag aaggacatcc ttgagttgtt taccttcaag ctctggaagc
    48841 agctctttac cagtatagtc agcaaagaca cgacgcacca gcgaataacc gtttgagtgg
    48901 ataccacttg aagcaagtcc caaaagaata tcgccttcct ttactttgga accatcaatg
    48961 agctgagatt tttcagccac accaacagca aagccagcaa ggtcataatc atcttcgcca
    49021 tacatcccag gcatttcagc ggtctcacct ccaatgagtg ctgctcctgc ttgaacacaa
    49081 ccttctgcaa cgccagaaac aacttcttca agtttgactg ggttattttt tcctgtcgca
    49141 atataatcta aaaaatataa tggctcagca cctgctgcaa taatatcatt gacacacatg
    49201 gcaacacaat cttggcctat ggtgtcatgc tgttgatatt taatcgcaag catgagtttt
    49261 gtgccaacac cgtctgttcc tgaaaccaaa ataggctctc taactcctgt tttactcaag
    49321 tcaaacaccc caccaaagcc acctagagct cccatgactc ccgcacgttc tgtacgagaa
    49381 acgtgttttt ttattctttc aaccacttca taaccagctt caacatcaac accagatttt
    49441 gcataagcat ttttttcaga catgttttct ccttatataa ttagcagagc ttatttcact
    49501 ttttgaatat aaaaacttgt ttcttcttct aaacttctga ggtattcttc ttcataatca
    49561 taaagcggtg ttggataatg tccatcaaag taagctacgc ataaaccacc atttggtgct
    49621 tttgtttcga gtccaattga ttcaatcaac ccatctagtg agagataggt cagactatct
    49681 gcgccaataa tatcacacac ttcgtcagca gcgtgatttg ctgaaattaa ttctcgtctt
    49741 gtttgaatat caataccata aaaacaagga tactttaatt ctggactagc tatagcaaca
    49801 tgtacttcac tagctcctgc ttctcttaat aatccgacaa tccttctaga agtcgttcct
    49861 cttacaattg agtcatcaat cataacaacg cgctttcctt tgacaacacc agataccgct
    49921 gatagtttca ttcgaacacc ttgttccctt aattcttgtg tcggttgaat aaaggttcgc
    49981 tgcgtatact gattttttac aagacccatc tcatttggta atccggattc ttcagcaaag
    50041 cccatagcag ccgataagga cgaatttggg acaccaatta caatatcagc atcctgttta
    50101 aattcttgtg caagacgctt tcccatattt tttcgagccg tatgaacatt aacaccatgt
    50161 atagttgaat ccggccttgc aaaatagaca tattccatcg aacaaattgc cagttgcgtt
    50221 tcatcagtat aacgatcaca ttgaatacca ctatcgtcta taaggataac ttcgccaggt
    50281 tcaacatctc taacccattt tgcgcctacc acctcaaaag cacaggtctc actggaaata
    50341 acccaagcac cattttgcat ttgtccaatt gacaaaggac gaaaggcatt agggtcaaga
    50401 gcagcaatta atttatcttc tgtcatcagt agataggcga agcctccctt tacagtgctt
    50461 aaagcttctt ttaccttccc cataaaactt gggttatggc ttcgacgaat caagtgcatc
    50521 aaaatttcag tatctgagga ggcattgaaa attgcacctt gcttttctaa ttctttcctt
    50581 aaagaaatag catttgtcaa attaccatta tggcataaag caaattgccc gtcatgaaat
    50641 ttataaagaa aaggctgaat attgcgaata tctgcagaac ctgcagtagc ataccgaaca
    50701 tgtccaatag ccgcattccc agttaaatta tctaattcag attgattctt aaaaacttca
    50761 gaaaggagcc caacatttcg ataaccatag agtttcccat tatcattcga aacaatacca
    50821 gcaccttctt gaccgcgatg ttgaaggcta tgaagcccaa agtaagtgac ttgagctgcc
    50881 tgaggatgcc cccagatacc aaagactcca cattcttcat ttagagattt tacttcgtat
    50941 gtcattcttt tttcctaatt ctttttgtat ctcatcttaa gtagctttga tgattttctc
    51001 aattttggat ctatcgtcac caatgccaac atcaaaatag aaaatctatc aagctatttt
    51061 caagctaatc attgagtctt agcctcaacg gctcccaaat accactacga cagaaagata
    51121 cagttaagct cgcttcgctc ttgtttcctt atctgtttta tgaaaaaatt tgaaaaaatt
    51181 attttcctgt aaaatatttt actgcactag caaacaaggc ttggtctttg ttaccaggga
    51241 tattttggaa gaggccgtct tcccagcgtt ctgagtgccc catcttaccg atgatttgac
    51301 cattcttgct ggtaatccct tcgatggcat tgacagagcc atttggattg tatttagaat
    51361 ccatagatgg ttgtccgtca aagtccacat attggctcca gatttgacca ttgtctctta
    51421 gctctgcaaa ttcagaagcg ctgacaacaa atttaccttc accgtgtgaa actgggatgg
    51481 catgaatatc gccgacctca actcctacca accaaggtga gttggtattt gcgatacgag
    51541 tctcaaccat cttggcaacg tgctggttgg catcgttata gaagagggtt ggacttgtct
    51601 caccagcttc ctcgaagttt ccgtatggaa gaagacctga tttaacaagg gcttggaaac
    51661 cattacagat accgatgata aggccacctt tttcgatgaa gctgtcaata gctgcgcgaa
    51721 ccttcttgtt aagcaagata ttgacgataa acttagcaga cccatctggt tcatccgctg
    51781 ctgagaatcc tccagcaaag aagatgatat ttgccttagc aatattagcg accattgtgt
    51841 caactgagtc agcaatagca gcctcattca aggttacaaa tggtaccaag ttgacactag
    51901 ctccaacctg ttcaaaggcc ttagctgaat catattctga gttggtacca gggaagactg
    51961 gaatgtaaac cactggtttt tcaattgttt ccttagcctt gataacagta tctgatacca
    52021 cagcaggaac ttcttcaaga gcgtctgcct gttcaaactc tgttgggtaa acctcttcca
    52081 atttgccttc gaagactgat agaaggcttg ccccagcaag gtcatttcca ttgacagtaa
    52141 gtgtaaaggc tgcctgagtt tgaccgattt tcaccgagtc agcaatttcc tcagctgatg
    52201 taaagacaaa gcctccgagt tgagctgtca agctactgtc aagctctgca atttcaacag
    52261 aggccccgat acggttacca aaagtcatga gagcaagact ttctaggaca ccaccgtatt
    52321 taacagctga agcagcagta atcttatgtt gagcttgaat agtctcgaac tggctaaagt
    52381 tagccttgat aaggtcaaaa tcaatatctt ctgaaatagc ttgacctgga atgtagtaga
    52441 tattctcacc agccgcttta aactcaggag agagaacctt gcggctgtcc gctgttgtca
    52501 caccgaaagc taccaaggtt ggcggtactg tcaattcttc gaaagtacca gacatagagt
    52561 ccttaccgcc gattgatggc aatccaagtt gaatctgagc ctcaatagaa ccaagaagag
    52621 ctgatactgg ctgatcaaaa cgctctgcct gtttatccat acgctcgaaa tactcttggt
    52681 aagagaaacg tgcacgagac cagtcagcac ccgttgctac caagcgagct gtcgcttcaa
    52741 tgacagcata ggcagcaccg tggtaaggtg accattctgc aatataagga ttgtaacctt
    52801 gagccataac agatgcagtt gttgtgacac cgtgttgaac tggcaatttt tgaacagagc
    52861 tttctgttgg tgtgatttgg taacggccac cgattgggtg gttaacggtt gagcgaccaa
    52921 cagatgagtc aaagatagtt tggagacctt tttgactagc gtggttgagg tcagacaaga
    52981 ccttaagcgt atccgcttca agtgtctctg ctgatgttgt gcgtacctct ggaactgtca
    53041 agtctttgtc aacgacttta gcatcaacaa cgacacggac accgttggta tcaaggaaac
    53101 ggcgttccaa gtcaacaatt gtttcaccat cccaagtcat gacaaggttt ggttttgcag
    53161 taatagtagc aaccacaacg gcatcgatat tttccttgtt gcaggctgcg atgaaggtat
    53221 ccacgtcact aggacgaaca acgactgaca tacgctcttg tgattctgag attgcaattt
    53281 cagtaccatt aagaccttgg tatttaagcg gtaccttgtc caaatcgatt tcaagaccgt
    53341 cagccaattc accgatggca acacagacac cacctgcacc gaagtcattt gatttcttga
    53401 ttaggcgagt gacattacca tcacggaaaa ggcgttgaat cttacgttct tcgatggcat
    53461 tccctttttg tacctctgcg ccagctgttt ccacagattc aaccgtttga accttagatg
    53521 aacctgtcgc accgccgaca ccgtcacgac ctgttttacc accgagcaag atgaccacat
    53581 cgcctgcttc tggtttttca cgaaccacat tttccttagg tgcagcacca accacagctc
    53641 caagctccat gcgtttggct acaaagccag ggtgaaagta ctcacgcaca taagttgtcg
    53701 caagcccaat ctggttacca tatgaagaat agccgtgcgc cgcagtttta gaaataacct
    53761 gttgcggcaa tttaccagca cgtgtttccg caatcggagt cgtaatatcg cctgcgcctg
    53821 aaatacgcat agcctgataa acgtatgaac gtcctgacaa cgggtcacga atggcaccac
    53881 cgatacaagt tgctgcgcca ccaaatggct caatttctgt tgggtgattg tgagtctcat
    53941 tcttaaacat gaggagccaa ggctctttta caccatcaac atctacttca atttcaactg
    54001 agcaggcatt gatttcatct gagacttcca tatcatccag tcgtccattg gcacgttcat
    54061 aacgaccaaa gatagtcgcc atatccataa gtgtctgcgg cttttcagaa cgaccaagtt
    54121 catcacgcat ggcgatatat ttgtcataag tcgcctgcaa ttgtttttgg aatttagaag
    54181 ctgaaaagtc gatgtttttc aactcagttt caaaggttgt gtgacggcag tggtctgacc
    54241 agtaagtatc caaaactttc aactcagttt cagttggcac acgcccgatt gatttgaaat
    54301 aatcttggat gaagagaaga tcatcgacct ccatagccaa gccctgctct gccttatagg
    54361 ccgcaaaatc gtcagcctta taagtttcaa agaaatcaag actagggatt gtcttatcag
    54421 acacagagaa agcctgctct tcaagcggca aggtaatgtc cttgaaacgt gaatcaacag
    54481 gattcaaaag atagttctta acggcttcaa gctctgcttc tgcaatatcc ttattgacca
    54541 agtaaagctg ggctgtattg accttaacct gactgtcact tccaagcaat agcaaagctt
    54601 cttgcgaact agcagcacgt tggtcaaatt gaccaggaag cgcctcaatg gcaaagaagg
    54661 caaccttatc aagctccgca gtgatttcag tttccgtcaa aagacagtct gtcacctgct
    54721 cagagaaaat atgcttctca gcacgcgcca gcaaatcctc agccaaattg aagacatcat
    54781 agacctgcac aatacgcaaa tccttcaaag aagtcagttg tagattatgc gtcaactctt
    54841 tcacaagact agccgattta atgtcaaagt cagccttttt ctcaacaaaa atacgtttat
    54901 tcatataaga taccaagtcc gacaagggag cgactgaaaa ataggaggat atctgaaatt
    54961 gagatttcag taagcccaat cttttttctg agtttcccga acggattcaa ttccttcctc
    55021 ttctaattac aatgctatca acttctcaag tacaacttga tagacatcgg tcaagctacc
    55081 aagatctcta cgaaaaacat ccttgtccat atgatttcct tcagcatccc atagacggca
    55141 gttgtcaggt gagaactcat cagccaagat gattttgcca tccttgtcaa atccgaactc
    55201 caacttgaag tcaatcaagt taagcccaat ctgagcaaac caatctttca agagttcatt
    55261 aatacggcga gtctctcctt tcagataagc aatttcttca tcattaacaa tacccaaaaa
    55321 cttcatatgc tcatcattga taaaaggatc attcagatca tcatttttgt agtaaaattc
    55381 cacaattgga gtttcaagaa cgcgtccttc ttccactcca aaacgttttg aaaatgagcc
    55441 tgctgtcaca ttccgtaata ccacctcaag tggaataata tcaacttttt tattcaattg
    55501 ttcgtttttt gaaatttgct caatataatg tgtcaccaca ccagccatat ttaatttctc
    55561 aaaaataaga gatgaaatct gattatttag tgctcccttg ccatcaatag tctccttacg
    55621 agctccattc aacatagtag cttggtcttt atagaccgtt ctaatcacat tttcatcttt
    55681 tgtactataa atatctttcg cttttccttt ataaataagc tggttagtca cttgattatt
    55741 cgcctttctc ttattttaag agttattcta acacacaaca ttatctaata caacaaaaac
    55801 tttatttaaa atcaaaaaaa agaagaaacg ttcgtatttt cttccttttt attattactt
    55861 atgttcaatg atataatcta atagttgttg gacgcttgac atgtgttcga cagcctcatc
    55921 tggaatctct agatgaaaca catcttccaa attgataata aactctgtta aagcgataga
    55981 gtcaattgct aaatcatcat gtaacgatga ctctggtgtg atatctaact gtgggtcacc
    56041 aagttgctgc cttaataatt ccagcatctt ttcaaaaact tcatctcttg acatgagttc
    56101 actcctattc atctgtaaag gcatcaacta attgatcaac tacttgagtt tccaacattg
    56161 tacgaacttg cttcaaagta gaataaacag ctttagaatc acttgatcca tggcatttta
    56221 ctattggagc ttttaaacca aaaagaaccg cacctcccgc gcttgaatag tccatactat
    56281 cttttagctg atacaggcta tcttttaata gaagtgctcc taatttagct ttcacgccac
    56341 ctgatttaat tgaagatttt agacttccca taatattcat agctgtacct tccattgtct
    56401 ttagaacagc atttccagta aaaccatcag tgacaacaat atctgctaca ctagacatca
    56461 aatcacgtgc ttcaatattt ccaataaaat taatagaagg ctctgctgct aacagttcat
    56521 aggcttcttt atgaagagaa tcgccttttg tttcttccgt tccgttattt aagagaccta
    56581 cacgaggaac ttcaatacca cggacatttt ttgcataaaa cgaccctaaa atagcatatt
    56641 gatgaagatg agaagctgtg ttttctgcat tagctcctaa atcaagcata tcaaaaccca
    56701 caccatctaa cgtaggcata gttgacatta agcctggtcg atcaacacct tttatacgtc
    56761 ctactacaaa caaaccagca gctaataaag ctcctgtatt ccctgcagaa ataaaggcct
    56821 gtgcaacacc atctttgact gccttagctc ctaagaccat cgaagactgt tttttacgac
    56881 gaacagcttt agcaggctca tcatcagaat taattttttc ttcagtatga ataatactaa
    56941 ctcgctccgt tactgttaaa tatttctcaa tttttttctg atcgccatag agttgtactt
    57001 caatatcact aaaatctgaa atagcttgat taacaccctc tacaatagct ttgggagcgt
    57061 aatctccacc catagcatct acagcaattt tcttcatgaa tgaactcctc ataaaaactt
    57121 agtggctata ttatagcatg tttctttatt tttttcacaa gaaggcaata atagctaata
    57181 tctaacagat actttcactc tttccaaaga aaagtatgct aaacaaccta ctaatatcaa
    57241 agaacatagt actccccctt caaaaccaaa atccccacca ctccaaatag caaagctaga
    57301 attttcagta attctaaaaa gagaaacagg agaatacccc ccactaacct tattacctaa
    57361 tacacatccc atagtaaaat tccatgcagt atgaactcct gtaacaaaaa gaatattttt
    57421 ggtcatatta aaaagaatag aaaaaataat accaagtaaa aataaattaa aaagtgctat
    57481 tgctgtgata ccatagtttc taaagtggat aaaggaaaac aacatagaat tgcataaaac
    57541 acctgcaaaa ctacctttaa atttggtaac tgttgtcata acatatcctc taaataaaat
    57601 ttcctctgtc aagctttgga taaagtatgc aagtaataaa aagaaaaaag gtccaaaaga
    57661 aaaggaattg ctggaagaaa tttccacaaa acctgataac cagtggcaaa tataaataa
//
LOCUS       DATRSG010000007        52046 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRSG010000007 DATRSG010000000
VERSION     DATRSG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52046
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.007"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            278..448
                     /locus_tag="VBD89_000367"
     CDS             278..448
                     /locus_tag="VBD89_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134923.1"
                     /translation="MDKELTPQEKANKKWAENNREHRTYLSKRSTARSFINKNATKED
                     LLELKQLIESKL"
     gene            complement(489..689)
                     /locus_tag="VBD89_000368"
     CDS             complement(489..689)
                     /locus_tag="VBD89_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7134924.1"
                     /translation="MSQEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
     gene            complement(844..1803)
                     /locus_tag="VBD89_000369"
     CDS             complement(844..1803)
                     /locus_tag="VBD89_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEO7134925.1"
                     /translation="MAENFQHKSYREQVELLESRGIVFSGRKAKSKAEYSLSVISYYK
                     IKEFAKPFAKIQKDGQKKKIDYQGTKFEIVISRYYQDKNLRLNLLHAIEDIEVAIKTK
                     IAYVLGKNGLGSYGYLDFSKWCNKEEYCKHYLSYSENNFKKQLKRELRKASSSELDEK
                     LKLDRQKYPPIWLAVNMLTFGQMVNLLELMSTTNLTQISSTFSCSNPELISWLKCINL
                     ARNICAHNSNIIDFKFITVSKLKEEWKDFLFEYKEGVFSNRIALPFLIVLEMMGKINP
                     KYHFRDIIDSLHKLIKDEDTAKYYGFASMNTIKEIKKKKNFRG"
     gene            complement(2142..2861)
                     /locus_tag="VBD89_000370"
     CDS             complement(2142..2861)
                     /locus_tag="VBD89_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7134926.1"
                     /translation="MVINIEQAIAWMASRKGKVTYSMDYRNGPSSYDCSSSVYFALRS
                     AGASDNGWAVNTEYEHDWLIKNGYVLIAENTNWNAQRGDIFIWGKRGASAGAFGHTGM
                     FVDPDNIIHCNYGYNSITVNNHDEIWGYNGQPYVYAYRYSGKQSNAKVDNKSVVSKFE
                     KELDVNTPLSNSNMPYYEATISEDYYVESKPDVNSTDKELLVAGTRVRVYEKVKGWAR
                     IGAPQSNQWVEDAYLIDATDM"
     gene            complement(2863..3201)
                     /locus_tag="VBD89_000371"
     CDS             complement(2863..3201)
                     /locus_tag="VBD89_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO7134927.1"
                     /translation="MNEFTTQIITVSVPIFGIIAGILTHEVKKLLIKKGGEKAVKIAE
                     IVARNAVEAVEQISAEVGGIKGQDKLTEAKTAVIDGLSQYNINLTQTQLETFIEAAVK
                     RMNDEWKKGQ"
     gene            complement(3203..3499)
                     /locus_tag="VBD89_000372"
     CDS             complement(3203..3499)
                     /locus_tag="VBD89_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134928.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNNNKEK"
     gene            complement(3509..3670)
                     /locus_tag="VBD89_000373"
     CDS             complement(3509..3670)
                     /locus_tag="VBD89_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134929.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDVVLELLS
                     QKVTDKESE"
     gene            complement(3733..4149)
                     /locus_tag="VBD89_000374"
     CDS             complement(3733..4149)
                     /locus_tag="VBD89_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO7134930.1"
                     /translation="MKQWNIVGKPFPIYQDGVVVKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIRLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            complement(4160..4312)
                     /locus_tag="VBD89_000375"
     CDS             complement(4160..4312)
                     /locus_tag="VBD89_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134931.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            complement(4321..8148)
                     /locus_tag="VBD89_000376"
     CDS             complement(4321..8148)
                     /locus_tag="VBD89_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7134932.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFNGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWTDQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNADNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVTCNDIGTIKKQTVAVSQLSSK
                     INNNQEILDNAVFKANQALASADGKNTNYYGTEMPVDDPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKNLSDSAKKDATEALTNLISESKKLTDKMSALSKTEVEHYSTTNLQLTQLDG
                     TVKGLQTSYEALLKKDGDITQTLANYKQTIDQNSTSITANKKTVDGTLSSLQTQVTQT
                     ANEITTRLSQTNIEALITSKSAVIADNKVKETADSFSREITRVNNTIDNLKIGTVNLL
                     SGTKNWTKNWFNRNNWQSDDDNLTIGNFSLTVLKRKAMWSGISQLYAVKSGENYVFSA
                     YAKSSIDNDVVNLYLLPNDQSQQAKISISFKSITLNTDWQRIVIKFNVTSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVTSDYSESPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVATVSQLDNNILPGTKKFTGWQTE
                     GAVLEDLTQSPYPFVFKKWVSGNKVSPLIEYDVKKDQEYTFTAYISREQAGNLYFYLY
                     DLWNHHITSTTPRETIIKDVTQTIRRFKITFIPTRDGKIRPRFAMLASDPGWFMVGGF
                     MLSKGTADLPWSESKQDIKDNVTAINTIVAQTANSWAIKNLTSNGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLTDLNTGQIEFYTDNPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKIKVGDIWLWKNTSTYTSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            complement(8161..9003)
                     /locus_tag="VBD89_000377"
     CDS             complement(8161..9003)
                     /locus_tag="VBD89_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO7134933.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSHTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            complement(9016..13242)
                     /locus_tag="VBD89_000378"
     CDS             complement(9016..13242)
                     /locus_tag="VBD89_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEO7134934.1"
                     /translation="MAGTFDGAIFADIGANTKDYEAAMARIVSTTQNAFRKAQDAAVN
                     NSNKMIQMIGQIMAQLANNGQSLGQRLGNAYSTGLKLSLGQIQRIAASIGEKIPEPIK
                     NGFNKAFSIIQNSVQKLSSAIPQPIKSAFTSATSAVVSFSSKITSAVSAAFNSVSSKA
                     STVADKISSSFGGRITSAVTNLATKLSTGLSNSFSRMSSSATSALNGISQRFASASSA
                     GEKLRSTVGSIISAFSLMAIAQKGIQAITGAMDGAISRVDTMNRFPKTMALFGYSAQQ
                     SKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTELALAFNNAMIGYGATTE
                     GAEQALRQFNQSLGSGKIYAEEFNSVSEAAPGLMSKMAESFGFGKNGVQDLKAALSDG
                     SITAQEFADKMIELNKAQGGFAEMAQSSAGGIRTAWKNVHTAVVKGVAGMISAFDEAA
                     KANGMKTIAESINSIKPAINSAFATVNSIMPNLVAAFARISKMVNIDFSPLTSGIKSA
                     FGLLNTALGEFAKTGEVSSATIDRIKSKIASIGPTAIAAWALLNPASAIATIMPLISI
                     IGKIGVALGTVGSALGSFGAMVASGLSTASGIIGAFAATVSGLPAIFATAAARGVAIL
                     GAMTQAMGAVVSVAMAAIGPAAILGLVVAGLGLVNNQFGTQIDKLLNIAITRGSQIIT
                     NLVNGITSQIPQLIASGAELISKFANAITVLLPVIIQAGVQLISSLVQGVGQNATSLI
                     SSAIQIIGTFISSIASALPQLLAVGMELIVNLVQGIVQNIPLIIQYAQQIINNFGASL
                     QANMPSIVNNGIAILTNLVQGIIQMLPAVVQIATQVITGFISGIAQYLPQVIQGGIQI
                     IIMLVQGIIQNLPQVIQAAMQIIQSLISGLSQALPQIISAGLQLVVQLAVAIIKGLPQ
                     IISAGIQLIIGLGKAMLEAIPNALSGVWEGIKGGFSSMWDQITGKSSTSAAKVTSDMS
                     TMTMNVGMQTTTMANQTNIDTTTMLNSISQNTGLANLNATTNATQMATNVNAQTASMA
                     NQGLLNAAGFNEALSSNMTLANTNATNQANQMTANVNNATSQMSLGSLPNFLELSQQA
                     TSNMQTAQANATTSANGMSSGVTGATAGMASGVANNMSGITSTVSTGMNQAANAAKSG
                     GANISNSVNSSFTQAKSTVSSSMSGMNSTVTSGMNSINNTVKSGGNNIKSTFTSSFNS
                     AKSTVSNAMSSIRSSISSGMNSAVSVTRSAMNSVVSAMNAAASGARSAGYNTGIGFAN
                     GLASTAGTIMAIANSIAANVAATIRSAMDIHSPSRVTRELGAYTGEGFALGMQDWMDD
                     INVIGKEYANAVTDQRWETDSQLATSANISSAGVSSSLDNLSDEVRNQQLTSPTFEVH
                     NELVGDEIYTTIKSKEARESNKDNYFNFAT"
     gene            complement(13271..13501)
                     /locus_tag="VBD89_000379"
     CDS             complement(13271..13501)
                     /locus_tag="VBD89_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134935.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNISNDFAERVAKLNLHFQNL"
     gene            complement(13576..14031)
                     /locus_tag="VBD89_000380"
     CDS             complement(13576..14031)
                     /locus_tag="VBD89_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134936.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDSAVVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEIENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            complement(14082..14714)
                     /locus_tag="VBD89_000381"
     CDS             complement(14082..14714)
                     /locus_tag="VBD89_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO7134937.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     TLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLKDREVYPAKFGYGKIDEIEYSEGVEDLVEASYALNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSSPGAVPEKLSEGH"
     gene            complement(14726..15085)
                     /locus_tag="VBD89_000382"
     CDS             complement(14726..15085)
                     /locus_tag="VBD89_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134938.1"
                     /translation="MIYSPSTLYLNEVKNRLGTLNIPIYFKLPKSDVLEPFIVIGSNV
                     SDTSKTAQTGAVVGDFGLNIDAFLPATSRTDAEEVKSRMIMSLGRNKRINAQLLMDNS
                     VGREVYHIAINITEIVI"
     gene            complement(15089..15433)
                     /locus_tag="VBD89_000383"
     CDS             complement(15089..15433)
                     /locus_tag="VBD89_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO7134939.1"
                     /translation="MSLTYRMRGLDRFLRSVERKQKSARIAVDKKLSKSAARIERQAK
                     ILAPVDTTWLRSQIYNEQQKLLHYRVVSPVLYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            complement(15430..15708)
                     /locus_tag="VBD89_000384"
     CDS             complement(15430..15708)
                     /locus_tag="VBD89_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134940.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            complement(15719..16075)
                     /locus_tag="VBD89_000385"
     CDS             complement(15719..16075)
                     /locus_tag="VBD89_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO7134941.1"
                     /translation="MDKELLLVEVKIFKGIAKNDASQDELIKLAIDESIERILAKLNE
                     YSDEELLEIPRRLTFIARDVAIKRYNRLNSEGAKADSEEGRSFSWEDYLTEYDSILRN
                     VAYGKRCRGKGVARFI"
     gene            complement(16081..16977)
                     /locus_tag="VBD89_000386"
     CDS             complement(16081..16977)
                     /locus_tag="VBD89_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO7134942.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRREELT
                     SDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAEAIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGAGLQKALSLSWAKLATFNEFE
                     GSQLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKVYST
                     AVDNLVFASLNVRGGDLGGLFADYTDETGLIAAARQRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEADKEVPGAVPGG"
     gene            complement(16996..17565)
                     /locus_tag="VBD89_000387"
     CDS             complement(16996..17565)
                     /locus_tag="VBD89_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO7134943.1"
                     /translation="MAEENVATETTEQVDTQKEAVEQAKQEHERTFTRADISKMMTAE
                     RAKWEAEQSDAIEKARTEGERLAKMSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     QTLLVEKGLPLEFIDVVLGTTAEEVKTNIDNLQIIFDKAVEKRVNNRLTQKPPRTGNG
                     SVGMTKAEIMAVEDDDERMRLIAENRNLF"
     gene            complement(17708..17911)
                     /locus_tag="VBD89_000388"
     CDS             complement(17708..17911)
                     /locus_tag="VBD89_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134944.1"
                     /translation="MNKRIKKKRKLERAIVMLIAENAMQAEALRNQHKRIEQLEQIVE
                     HNAQATNNELDYIKNNYKKKWKK"
     gene            complement(17916..18104)
                     /locus_tag="VBD89_000389"
     CDS             complement(17916..18104)
                     /locus_tag="VBD89_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134945.1"
                     /translation="MGVRPPSFTTISIHQYSRIIKEEDMFIWRMVLVALGVLVLILII
                     GIAAIAVKSIIAEVKKEG"
     gene            complement(18132..19580)
                     /locus_tag="VBD89_000390"
     CDS             complement(18132..19580)
                     /locus_tag="VBD89_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEO7134946.1"
                     /translation="MLMDKKNQQQDHLDYWQKRQEDIVSYLDRADLDIFNELSRVYSN
                     QSLSLQKDLYDFYNRFAEDNKITYQDAVKKLRHEDLSDYAANANRYREQAEKDPELLK
                     RINEQYVSAQATRLDMLNLELIHRAGTMRGILDGLFSDHLRKAASYAYQKSVGGRSGT
                     INGPALEQLIKTPFDGYNYSEQLWGNVDNLVKDLQEAFKKGFVRGLGPREMARELGKR
                     YDVARHRAETLIRTDGTMIINNATAKRYIDAGIKYYRDLVQLDDRTTQICRSKARENK
                     KKLLSEIQPGINAAPYHFNCRTTIIPDEDELNTDVEPIADKSTKYFKDVTSDWIDGQE
                     YESQISMLNEYTKGGGTYRVDGHNVVLDHSNYEYRVANWLSKKTGLQVEMVPRVNFPE
                     NINTPDYLVDDVPFDLKEITGSGKNVIDGNLRKAKKQAPNIIFDITKTPLSFEEILGQ
                     LEHIYKIDRRGLNISIIKNKDEVLAVVEKERR"
     gene            complement(19540..21069)
                     /locus_tag="VBD89_000391"
     CDS             complement(19540..21069)
                     /locus_tag="VBD89_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO7134947.1"
                     /translation="MFERKFLSGTRFNENANRQFIMLQEDFDAIEYNSQKWIDQLKKY
                     ISSFETEQLDRLKELKRYYLGDNNIKYRPAKTDEYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNEDSDIQKNINLMSVRNNEDYHNVAIKTDLSIYGRAYELLTVEEVDNGTEI
                     KLYQLPAEQTFVIYDDTYQHNSLMAVHFYDIDYGAGKRKQIVKVYTSNMIFTYEDFNQ
                     ETKGMSLKDEEEHYFSGVPVNEYSNNDDRAGAYESVLDNIDAYDLSQSELANFQQDSV
                     DAILVLSGNPYTGADENDFFEDGRINPNGRLGISIGFKKAKVIVLDDNPNPDGAKPDA
                     YYLKKEYDMAGSEAYKNRLVSDILRFTFTPDIQDLKFSGVQSGESMKYKLMASDNYRE
                     KQERLFKKGLMRRLRLAANIWSIKGNESTVYSQVNDTSIVFTPNVPQNDNEIVSAAQS
                     LYGIVSDQTIFEILNAVTGVDAEAELKRLKEEADEKQVMPEPRNVGDANGQEEPTAGP
                     S"
     gene            complement(21084..22364)
                     /locus_tag="VBD89_000392"
     CDS             complement(21084..22364)
                     /locus_tag="VBD89_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO7134948.1"
                     /translation="MTTKQRPKINIVIKYPSKVFNKHIYDKLYNYDNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGVLDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQVYLMFNPVSKANWVYKAFFIKSPKNTVVYQTTYKDNRFLDDVTRENIEELAN
                     RNEAYYKIYALGQFATLEKLIFPKYEKRILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKSLGYAKEEIRGDSAEKKSNQELRNLGIQ
                     RMIDVKKGAGSVMQGIQYMLQYDIVVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSSDRNKRLNNAKYYFR"
     gene            complement(22354..22785)
                     /locus_tag="VBD89_000393"
     CDS             complement(22354..22785)
                     /locus_tag="VBD89_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO7134949.1"
                     /translation="MSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARSIGQENLT
                     KPDIKAYIDERIEKLESAKIATQEEVLQYLTSIMRGDQQEKTLISIGELGQEIVDIDV
                     GAKDRIKAAELLGKRYRLFTDKIEMDISSDVTISVGEWDDD"
     gene            complement(22906..23319)
                     /locus_tag="VBD89_000394"
     CDS             complement(22906..23319)
                     /locus_tag="VBD89_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7134950.1"
                     /translation="MSKLSNAQLKAFDEWLFDYRDIERKIAIRKLELQTPVSTDINVG
                     GGKSNLVAKETEDIVIKWSKDGKIKSYENFRESVDRVKALLDDELNYIFDLRWGTGSN
                     NTWEEIAYKIHTSRAGTYRKRDRILTLFAQQIGKL"
     gene            complement(23397..23729)
                     /locus_tag="VBD89_000395"
     CDS             complement(23397..23729)
                     /locus_tag="VBD89_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134951.1"
                     /translation="MSLIDEVKECGSESHKKWFDRYFDGLKIEEKLKQSAMKGFSGYK
                     ISIPKNDEYLARRLDSQKTIDLLKYRLGDGFDVEIKNLESSYKIFGKPVVLSRDLFIF
                     WRTGDKNG"
     gene            complement(23732..23962)
                     /locus_tag="VBD89_000396"
     CDS             complement(23732..23962)
                     /locus_tag="VBD89_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134952.1"
                     /translation="MNEQNNKLHKQSIVIVVLTVAVSVLLTTNIALYNYYQPQITGLQ
                     QQLIRTQYRLKESSEQNQRQTKRIAELTGNGG"
     gene            complement(23959..24195)
                     /locus_tag="VBD89_000397"
     CDS             complement(23959..24195)
                     /locus_tag="VBD89_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO7134953.1"
                     /translation="MTGELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLVEYWLLESVNE"
     gene            complement(24192..24407)
                     /locus_tag="VBD89_000398"
     CDS             complement(24192..24407)
                     /locus_tag="VBD89_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134954.1"
                     /translation="MKIDYIDFFERVVPGWMRESNKKMQELGFGTEQYWVWVNMSIVD
                     ICESYGNDDLVNGQFHMIWEWLERKTK"
     gene            complement(24404..24802)
                     /locus_tag="VBD89_000399"
     CDS             complement(24404..24802)
                     /locus_tag="VBD89_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO7134955.1"
                     /translation="MTIEFFIPMKKIPTTTHQQKKVRVVNGKPQFYEPDSLKEARAMF
                     MDKFAQHVPKEKLNGPIRLTTKWLFPKIKKSVNGQYKDTKPDTDNLNKLPKDCMTELG
                     FWNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            complement(24829..25026)
                     /locus_tag="VBD89_000400"
     CDS             complement(24829..25026)
                     /locus_tag="VBD89_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134956.1"
                     /translation="MKEYKKRMIVKYQELKSRAEKLSVLINKFYIDSLDFKLQCPIEL
                     LVAQWHAMGAYLKILEQRAAI"
     gene            complement(25396..27675)
                     /locus_tag="VBD89_000401"
     CDS             complement(25396..27675)
                     /locus_tag="VBD89_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7134957.1"
                     /translation="MAENDFNLLPLLDYINPATVDYQTWVQVGMALKHEGYTAMDWDV
                     WSQSDSRYKKGECFAKWDSFQGNGFGTITGATITQLAKDNGWTSSEYRNSDDAHELSW
                     DDTIDRDYKIVDKNWIESKEIQEPRNWNPVQDLITYIDTLFESTDKVGYVTETYPITL
                     DTGEIVYKPTKGAYDRTAGQLIESLQKNPTDLGAVFGDFKEEAGAWIRFNPLDGNGVK
                     NDNVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSLHAIVKVDARDYQEY
                     RKRVDYIYQICKKNGLDIDTQNRNPSRLSRMPGVTRNGHKQFLIDTNVGKTNYEEWYQ
                     WIEDLNDDLPDPETLADEWDNMPELAPELIKGVLRQGHKMLIAGPSKAGKSFALIELS
                     IALAEGKEWLGWQCEQGKVLYVNLELDRPSALHRFRDVYEAMSLPPANIKNIDIWNLR
                     GKTVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQMAHFTNQFDKVATEL
                     GCSVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELELTEELIKSRSEKAA
                     AKIYQQALQEKALAYYQQEVTLDDLESRYQMQQHFDKAIKDIMIKQPYLEAVKKAQYE
                     VEISTAWRVEGTLREFAKFQPVNMWFSYPVHDVDTTGVLADISLEDNVPTWKKNFEKK
                     SPRESREKKSQKVETAINSLNDGIEPVTIDNLIEYFSTEDKPVSEKTIRRWIKENGKF
                     EVKNKQILPIEEPKTNKSL"
     gene            27746..27997
                     /locus_tag="VBD89_000402"
     CDS             27746..27997
                     /locus_tag="VBD89_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134958.1"
                     /translation="MPEIKITFSDGSIVIFHEEQSFQSVVKSNDAMSLSKIYSLWNHV
                     HDGLIPSFLELIANSQFFFDLENPQIYYASNAVIKIEAI"
     gene            complement(27990..28187)
                     /locus_tag="VBD89_000403"
     CDS             complement(27990..28187)
                     /locus_tag="VBD89_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134959.1"
                     /translation="MENINEQIEKFISNFADEAIEKSETYSEAILYVDKHSSLTEFGQ
                     VVKKAIQEKIRDIALNSRIIK"
     gene            complement(28196..29779)
                     /locus_tag="VBD89_000404"
     CDS             complement(28196..29779)
                     /locus_tag="VBD89_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO7134960.1"
                     /translation="MKLREYQEEARSAVQQEWEQGKKKTLLVLPTGCGKTIVFSKIIE
                     DRVKLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAESTSLGSWYRVVVGSVQTL
                     QREKRLSKFPTDYFDTIVVDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLTLDLSTVSQQAGDFKASEIGTALDPYL
                     EQIADEMVKQCSNRKTVVFLPLVKTSQKFRDILNQKGFKAAEVNGESKDRAEVLEDFD
                     NDKYNVLCNSMLLTEGWDCPTVDCVVVLRPTKVRALYSQMVGRGTRLAPGKEKLLLLD
                     FLWHTERHELCRPAHLIAKTPEVAQKMVENMTDETEVAFELLEAEEVASKDVVAEREE
                     ALAKQLAEMRKRKRALVDPLQFEMSINAEDLVDYVPEFGWEMAPASEKQLKTLEKFGI
                     FTDEIGNAGKAAKLLDRLDKRRQSGLTTPKQIRMLERYGFKNVGMWKFEVASNMINRI
                     AASGWRLPKGINAREYQPE"
     gene            complement(29785..30393)
                     /locus_tag="VBD89_000405"
     CDS             complement(29785..30393)
                     /locus_tag="VBD89_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134961.1"
                     /translation="MGKAKDYTGQRFGRLVVIERLPQDPYTKAKYKCQCDCGKLTIVN
                     SSNLATGHSTSCGCIVTKHGFARKERLYNIWVGMRQRCRDKNSNDYIKYGGRGITICD
                     EWEEYIQFKQWASSNGYRDNLSIDRIDVNGNYEPSNCRWANSTEQANNQRNNHLITFK
                     GKTQTIKQWSSELGISNITLLTRLNRGWSITKALTTPTQKGV"
     gene            complement(30393..30998)
                     /locus_tag="VBD89_000406"
     CDS             complement(30393..30998)
                     /locus_tag="VBD89_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134962.1"
                     /translation="MTEYNNNFEREFGWDDTIQEDAKEFITLQPGDYVFTVTDFERAR
                     HTPNPQKTGKLPACNKAVISLEIETDEGVASLKHNLFLHSSTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVVGATGVCSVKNRTYNDNVYNEVKSMIYADSVDWTKVLNANTQPQAPQ
                     PTYQQPAQQTTNFAPQQQPAPQSYQQGQMQASQQQGGWGGF"
     gene            complement(31094..32167)
                     /locus_tag="VBD89_000407"
     CDS             complement(31094..32167)
                     /locus_tag="VBD89_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7134963.1"
                     /translation="MQITRGKRARAQRVIIYGPEGIGKSSFAAQFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAFVKANPTCCKTLVIDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNMLQELIELGINVVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTADNNKKKATGGTRVLYTQHHPAWDAK
                     NRHGLQEEMPLDYAGIAHIFAQAPSQPVAETPPVQQPVPTQPIHEPVHEQQAQMSGPT
                     AATEYPSVLPKALTDLMTPEQVTPDELVAVANIRGHFPPLTPIENFPPDYWNMIVANW
                     GATLEVIKTQVRATEQPFTVEGS"
     gene            complement(32182..33501)
                     /locus_tag="VBD89_000408"
     CDS             complement(32182..33501)
                     /locus_tag="VBD89_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7134964.1"
                     /translation="MTITINKLEIENVKRIKAVKVEPSSKGLTIIGGNNNQGKTSVLD
                     SIAWVLGGNKFKPSQAAREGTMVPPTLKITMSNGLIVERKGKNSSLKVIDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDGTPKEKADVLLEIIGVGDQLAELELKEKELYNQRHTIGV
                     IADQKEKFAKEQPYYNDAPKELISIADLIQQQQEVLAKNGENARKRQNLSVIQQNHQF
                     KQSEVEHLKQQLATAEKQLQELSSDLEIAQRDTMSLIDESTAEIEENISNIEEVNRKV
                     RANLDKDKAEEDAKHQREQYNILTNDIESIRQQKRDLLINADLPLEGLSVDDGKLLYL
                     GQEWDNMSGSQQLIVATAIVRKLKPDCGFVLIDKLEQMDNITLEQFGKWLEQEGLQAI
                     ATRVSTGEECAIIIEDGYSVENKAHSFKTAEGGSFAETVAPTWQGGF"
     gene            complement(33485..33715)
                     /locus_tag="VBD89_000409"
     CDS             complement(33485..33715)
                     /locus_tag="VBD89_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134965.1"
                     /translation="MIEVERQPKNTYSRIRCSNNIYDEIANIANECDLTLKEVTDALL
                     NYGLANAQIISSEKVVTESKLVIGDFDNDNHN"
     gene            complement(33708..33935)
                     /locus_tag="VBD89_000410"
     CDS             complement(33708..33935)
                     /locus_tag="VBD89_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134966.1"
                     /translation="MLKEVLLENELLRDENRRLTNEMADYYFTNVAKANLLDIIVNEG
                     YILDSTLEKCIAKLDNVDRQTLEKNWSDDND"
     gene            complement(33929..34078)
                     /locus_tag="VBD89_000411"
     CDS             complement(33929..34078)
                     /locus_tag="VBD89_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134967.1"
                     /translation="MKKLFKKLFVKKNQVIETRQKWTIETHGWEANARRYDNMIERNN
                     RGRTC"
     gene            complement(34083..34394)
                     /locus_tag="VBD89_000412"
     CDS             complement(34083..34394)
                     /locus_tag="VBD89_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7134968.1"
                     /translation="MPKAELVYRPAKQSEKADYGDYVHLCQIWEGLTVGTAKVWAAEM
                     RNHPDFKQFINNPTHRIVFINYEGFKLFVTWKSRNRYKPKKETLAEMLENIKKERQLG
                     V"
     gene            complement(34474..34665)
                     /locus_tag="VBD89_000413"
     CDS             complement(34474..34665)
                     /locus_tag="VBD89_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7134969.1"
                     /translation="MPDIAVGREKVVTFLKSNNIKKSDLAAAYGLNRQEVTNILSGST
                     RGPKANQFILRVIADYSIE"
     gene            34716..35504
                     /locus_tag="VBD89_000414"
     CDS             34716..35504
                     /locus_tag="VBD89_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134970.1"
                     /translation="MSRYSDYKYANTILKSIEERPEKYLIIHYSCESFYNLGSKSPRI
                     ASISVRQFNNAQTNNFSIHQYSEILDTPITDDTYETIEKELLTDFFTFVDRNTDKNWI
                     HWNMRDTVFGFHAIEQRFKVLGGTPIIIDDDKKIDLAYLFKKMYGGGYIDNPHIEKLL
                     ELNNLTPHRFLTGKQEADAFSSRQYHELSMSTSSKVNSFSTFLTLAINDDLKTNTPKW
                     KMRGTNLSGLLSAFQETTSGKVIIGIINLIVGGIVGAIIAKYIQ"
     gene            complement(35455..35700)
                     /locus_tag="VBD89_000415"
     CDS             complement(35455..35700)
                     /locus_tag="VBD89_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134971.1"
                     /translation="MKKNIVDLQKRNEHFQWVADSLEGKENELYVERDWYDNPTLISK
                     EDAKKEVEQVQQKLILLQKKSFIEYILQLLHQLFRRQ"
     gene            35996..36343
                     /locus_tag="VBD89_000416"
     CDS             35996..36343
                     /locus_tag="VBD89_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134972.1"
                     /translation="MFPTFEKIKELCKQRGITLIQLEETLGYSRNTLYKLKTQKPNAE
                     RIAEIADYFNVSTDYLLGRTDNPNIAKDSQEYTSDDLRKMAEKAKTFDGKPLTESDID
                     AIQNIIEIYLKGR"
     gene            36347..36733
                     /locus_tag="VBD89_000417"
     CDS             36347..36733
                     /locus_tag="VBD89_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO7134973.1"
                     /translation="MTIEEIVDCHKVNLAYFDNELWPRPGIYIDDIKVIFVNKSLSLD
                     AQKRVILHELGHCERNIGDYKTLREKHELEADRYMISHLIAEALSQLEDKREFNYLQF
                     MQYHQLTTTADECMVIDEYYNLLKVV"
     gene            36751..37302
                     /locus_tag="VBD89_000418"
     CDS             36751..37302
                     /locus_tag="VBD89_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134974.1"
                     /translation="MDINKIKEFAVSTSEKAKDGIIKANEMRKRASQESKIVLPPTIN
                     LGFNTPTAIRKTVEGDYYIGFYSEKPDLFKFVDFQFDGSRIIEKTVTKGKTTQKGRSG
                     STIIGGLIGATINPTAAVLGGLAGANRGKKGTINSTSTTITEEIPGKAQLTLRNLKTN
                     DIKIIKTKLTQAQANNIQNFFSK"
     gene            37417..38559
                     /locus_tag="VBD89_000419"
     CDS             37417..38559
                     /locus_tag="VBD89_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7134975.1"
                     /translation="MWSEKHKSGKVNFVERYKDPYTNKWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTNLIDEWWIFYQQEIKRSSIVTLKGNIREIRAEFGIN
                     IPVVNIDPRYVQNYLDNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPKI
                     KKTLNDWKKIEEKYLEEEEIKRLLKELFRRPSTHRLGFLSEFMSLNGCRIGEAISIEP
                     DNIDFKNKTLQLHGTYDRTNGYINGEKTSPKTLASYRETIMSKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTRNGVPIQINSFNLALKKANERLEQPINKNITSHIFRHTLVS
                     RLAENNVPLKAIMDRVGHADAKTTVQIYTHITKKMKSNIADIMENY"
     gene            complement(38647..38922)
                     /locus_tag="VBD89_000420"
     CDS             complement(38647..38922)
                     /locus_tag="VBD89_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO7134976.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(39025..39627)
                     /locus_tag="VBD89_000421"
     CDS             complement(39025..39627)
                     /locus_tag="VBD89_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO7134977.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVLQYIKSSYKLPNFVDIKPK
                     KSVININLQDLKNKEGIYLKATAIDLVNDNFSFDIFKKKP"
     gene            complement(39602..40441)
                     /locus_tag="VBD89_000422"
     CDS             complement(39602..40441)
                     /locus_tag="VBD89_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO7134978.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(40434..41270)
                     /locus_tag="VBD89_000423"
     CDS             complement(40434..41270)
                     /locus_tag="VBD89_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7134979.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(41383..43041)
                     /gene="recN"
                     /locus_tag="VBD89_000424"
     CDS             complement(41383..43041)
                     /gene="recN"
                     /locus_tag="VBD89_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO7134980.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSIEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(43053..43526)
                     /locus_tag="VBD89_000425"
     CDS             complement(43053..43526)
                     /locus_tag="VBD89_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO7134981.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(43513..44340)
                     /locus_tag="VBD89_000426"
     CDS             complement(43513..44340)
                     /locus_tag="VBD89_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO7134982.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(44333..45205)
                     /locus_tag="VBD89_000427"
     CDS             complement(44333..45205)
                     /locus_tag="VBD89_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7134983.1"
                     /translation="MVTIEKIDEAIHRHYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(45205..45420)
                     /locus_tag="VBD89_000428"
     CDS             complement(45205..45420)
                     /locus_tag="VBD89_000428"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO7134984.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(45398..46738)
                     /locus_tag="VBD89_000429"
     CDS             complement(45398..46738)
                     /locus_tag="VBD89_000429"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO7134985.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLLPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATTVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRLHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(46864..47700)
                     /locus_tag="VBD89_000430"
     CDS             complement(46864..47700)
                     /locus_tag="VBD89_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO7134986.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNRTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(47697..48551)
                     /locus_tag="VBD89_000431"
     CDS             complement(47697..48551)
                     /locus_tag="VBD89_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO7134987.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(48690..50384)
                     /locus_tag="VBD89_000432"
     CDS             complement(48690..50384)
                     /locus_tag="VBD89_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7134988.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENIYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADVLLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQNPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(50671..51405)
                     /locus_tag="VBD89_000433"
     CDS             complement(50671..51405)
                     /locus_tag="VBD89_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7134989.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKKVFEQTKEIRTRDFLSKVL"
     gene            complement(51398..>52046)
                     /locus_tag="VBD89_000434"
     CDS             complement(51398..>52046)
                     /locus_tag="VBD89_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7134990.1"
                     /translation="GVMVTIMISTCVVFFGTIIGVLIALVKRTNLHFLTILANFYVWV
                     FRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIIIISLNSGAYISEIVRA
                     GIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNEFITIIKDSALLQTIG
                     VMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQMEKYLGKGVKIDG"
BASE COUNT        15318 a        10619 c         8155 g        17954 t
ORIGIN      
        1 tagaaaggag gaagatatga gaatatcaga aattgctgat ttgcttactt caatcggaac
       61 tctgttggtt ggtatagcaa gcataatcac agcaataaaa aaagaaccta aaaagaaaaa
      121 ccggccacgg agattcaaat aaggttctag tagtagtttg gggctcaagc cccttgccac
      181 tactgatagt atatcatatc taagacaaat atgaaatatt tgattatttt cgcgatttgt
      241 ttagttgtat tttactttat taataaggat gattgaaatg gataaagaat taacacctca
      301 agaaaaagca aataaaaagt gggcagaaaa caatagagaa catagaacct atctatcaaa
      361 acgatctact gctcgtagtt ttattaacaa aaatgctaca aaagaagact tattagaatt
      421 aaaacaatta attgaaagca aactctagac acacaaaaaa ccgccctcaa ttagagagcg
      481 gttaatattt atttcagttt ttctttgaca gcatctactg cctcttcaac agcatcttta
      541 gcatcatctg ctagttcttt gcctttagca attgtttttt cgacaaatcc ttttgcttct
      601 aactctttat caccggttag cttccctgca ccttctttaa gactgcctga cgcttgttca
      661 acttttgctt ttagtttttc ttgtgacata atgtggctcc ttattatttt tattctatgg
      721 taacattttg attatttcta ggcaaataaa aaagcaagaa ccgctagtgt caggcgattc
      781 ttgctagtgt gattatctca tgattatgcg agggtgtcaa taaggggaag agaaatccaa
      841 gcgtcagcct ctaaagtttt tctttttttt gatttccttt atagtgttca ttgatgcaaa
      901 gccataatat tttgctgtat cctcgtcctt aataagttta tgcaaagaat caatgatatc
      961 tcgaaaatgg tatttaggat ttattttacc catcatttcc aagacaatta agaaaggtag
     1021 agcaatcctg tttgaaaaaa caccttcttt atactcaaac aaaaaatctt tccactcttc
     1081 ttttaactta gagacagtta taaatttaaa atctatgata tttgaattgt gggcgcaaat
     1141 attacgggct agattaatac attttaacca agaaattaat tcaggattac tacatgaaaa
     1201 agtgcttgaa atttgagtta aattagttgt tgacatcaat tctagcaaat taaccatctg
     1261 cccaaatgtt aacatattta ccgctagcca aataggagga tatttttgtc tgtctagttt
     1321 taatttttca tccaattcag aggatgatgc ttttcttagc tctcttttta actgtttttt
     1381 aaagttattt tcactatagg aaaggtagtg tttgcaatac tcttctttgt tacaccactt
     1441 agaaaaatct agatagccat agcttcctaa gccgtttttc cccagaacat aggcgatttt
     1501 tgtttttatt gctacttcaa tatcttcaat cgcatggagt aaatttaatc tcaaattctt
     1561 atcctgataa tatctagata taactatctc aaatttcgta ccctgataat ctattttttt
     1621 cttttgtcca tctttttgaa tttttgcaaa aggctttgca aattctttta ttttgtagta
     1681 agaaatcaca gataaactat actctgcttt acttttagct tttcttccac taaagacaat
     1741 acctctagat tccaaaagtt caacttgttc tctgtaactt ttgtgttgaa aattttccgc
     1801 catgaaacac cctcaatttt ttagcaaaaa aaccccccat cagaacatgt ctgcgcgtat
     1861 gcgagggagg ccattgatat ttatatatac attatatatt tttatatagc ttattgtcaa
     1921 tgattttccc tttatataat taaacttttt aataaaatat atttttcatc cagtaaaagg
     1981 cacctaaata cctcctgaaa aatctgttca aaataataaa cagcccccgc aaaagcgagg
     2041 gcatttgtct tatctaaagg aattttacct cctaaattgt ttttttagtt gcggtgtaac
     2101 ttacaccatt aacagaccga catttatatc gctctgttgg tttacatatc tgttgcatca
     2161 attaagtaag catcttctac ccactgatta gactgtggag cgcctatcct tgcccagcct
     2221 tttacttttt cgtaaactcg aactctagta ccagcaacaa gcaactcctt atcagtgcta
     2281 ttgacgtcag gcttggactc aacgtaataa tcttctgaaa ttgttgcttc gtaatatggc
     2341 atatttgaat tacttagcgg tgtgttaacg tctaactctt tttcaaattt agatacaact
     2401 gatttattat caaccttagc atttgactgt ttcccagagt aacgataagc gtaaacatac
     2461 ggttgaccat tatatcccca gatttcatca tggttattca ctgtaattga gttataacca
     2521 taattacagt gaataatatt gtctggatca acaaacatac cagtatgtcc aaaagctcca
     2581 gccgaagccc cacgtttacc ccaaataaaa atatcacctc tttgcgcatt ccaattagta
     2641 ttttctgcaa taagaacata accgtttttt atcaaccaat cgtgctcata ttctgtattt
     2701 actgcccagc cattatctga tgcgcctgct gagcgtagag caaaatagac agagctagag
     2761 caatcgtaag atgaaggacc atttcgatag tccatcgagt aagtaacttt accttttcta
     2821 gatgccatcc atgcgatagc ttgctcaata ttaattacca tattattgac ctttcttcca
     2881 ctcatcattc atgcgtttaa ccgcagcttc gataaaagtt tcaagttgtg tctgtgtaag
     2941 attaatatta tattgcgata aaccatcaat aacagcggtt ttagcttctg ttagcttatc
     3001 ttgtcccttg ataccaccaa cttccgcaga tatttgttca actgcctcaa cagcattacg
     3061 agcgacaatt tctgcgattt taacagcttt ttctccacct ttttttataa gtaatttttt
     3121 tacttcgtgc gtgagaatac ctgcgatgat accaaaaatt ggtactgata ctgtaatgat
     3181 ttgtgttgta aattcgttca tgttatttct ccttattatt attaaataat gttttgattt
     3241 gctctttatt gatgatgata tcatcctcga cacgacctaa acgctcctcg tgacgatcaa
     3301 taacagcttt tgtaatctct cggtctctat ctatattttt gagctcgtat gctagttctt
     3361 tgagtgagtc cttgagctga gccatagcaa tctcgttagc ttccattgct tttttaaagg
     3421 gattaacaat aaatccccat actcccaaaa tagataaagc agcacctgca aaagcgccaa
     3481 cttgcacaaa atctatcatt aagttacctc actctgattc tttatcagta actttttgac
     3541 taagtaactc aagcacaaca tctcgtaaat taaataattt aggtactgct tcgaaatcac
     3601 aaataccatt cgcaacacga cggtaccaaa cgtcaacaat aagatgatct tttttaaaag
     3661 tatatgtcat attaaattct ccttttttta ttaaataata tagtgttata acggtattca
     3721 acgttaattc cattagcttg attccatcga tgtagacggc acggaaaggc tagatacact
     3781 tgatgtcact aagtcagtta actcgacaac tgctttatca actttaagct gcatatcttt
     3841 tgttgcttgc gacatcttag aaattgcctc atcagcctct gcaatttttt tgattaaagt
     3901 tactttgtca gcttttagtt gctcaatatc ttttacagac tcaagcattg cgaaatcaac
     3961 gacattttct tttgcaaaag tttcaagtac aagtcttatc agtgattgat tatccttatc
     4021 aacatgatta ccaattagct cttgtggtaa atatgtacca tcgtcgcctt gtaagcgtac
     4081 atccgttttt acaaccactc catcttgata gattggaaat ggttttccaa caatgttcca
     4141 ttgtttcatg ttactcacct cactttgctc ctggcttagt tatctcatct aattgctgat
     4201 ttaactcagc aatttgtaat tgtaattgct cattttgagc ttgtagagtt gctttatcta
     4261 aagataattg cgctatttgt aaagctaaat ttgattttat tttttcttcc attttttctc
     4321 ctaaatcttt cctggaatag tataactgta gcctttttca gtcgttttat tattgtgtag
     4381 tagctgcaag ttatcaataa tcatattaat tgtatctttc atgcttgtat aagttgatgt
     4441 atttttccat agccaaatat ctcctacttt tatcttagac gttacacgat gatttatgtt
     4501 acgtgcatct attgatagct gatttggcaa agtaatgatt tcccaaccat cagcgttagt
     4561 gtacgcagag cttgctaagt gaatactatc gccaactaaa tcaagagtat cgatgtcttt
     4621 accattccaa gcgcgaatac ctacgaaacc accgtcgttt gacgattcac ttccccatcg
     4681 attagaacct ataaccgtta cgccagccgc gccctttcct tcaacattac ctgttgcaaa
     4741 tttaacgaat tggttaggat aacctgcagt aatacgtttg atagctggat tatctgtgta
     4801 aaattcaatt tgtcctgtat taagatcggt taacattgca ccgttttgcg atattaaaac
     4861 gccgctacgt atcaggtccg ctgacattct tccgctggta atattactag catctaaatt
     4921 aatgacattt atacgattag catcaattgt tcctgctgta atcttatctg cagttaaatc
     4981 ttttatcatc gcattagtaa taatgccatt atcaataata gattgacctg acaaatgtat
     5041 taacttacct tccagtttta cgttaccatc agtcaaatta agctgactca atacagtacc
     5101 gttagaagtc aaatttttaa tagcccaact gttcgctgtt tgtgcgacta ttgtattaat
     5161 cgcagtaaca ttatctttga tatcttgctt ggattcagac cagggtagat cagcagtacc
     5221 tttagataac ataaaaccac caaccataaa ccagcctgga tcactggcca acattgcgaa
     5281 ccgtggtctg attttaccgt ctcgagttgg aataaatgtt attttaaatc gtctaatggt
     5341 ttgagtgacg tctttaataa tagtttcacg aggagttgtg cttgtaatat gatgattcca
     5401 aagatcatac aagtagaaat atagatttcc tgcttgttct ctagaaatgt atgcagtaaa
     5461 cgtatactct tgatcttttt tgacatcata ttcgattaaa ggtgacacct tattaccgct
     5521 tacccatttt ttaaaaacaa atgggtaagg cgattgtgtt agatcctcta gtactgcacc
     5581 ttctgtttgc catcctgtga atttttttgt gccaggtaaa atgttgttat caagctgact
     5641 aactgttgca actataccgt cagccgtttg ggtaactcgt gacaagcgtc catccacatt
     5701 agatactgtt gattgtatat tatcaacttt ttgtattgta gctgtcagac tctcgttttg
     5761 tttaccaatc agttgactat gactattaac tgtatcgttg attttattaa attcaacagt
     5821 cattttatct tcttgatccg ctggggactc tgaatagtca cttgtaacat ttccatgttc
     5881 aagctggtaa cctgcaatat acaaccaacc attttcatta aagcgttcga agcgtggtaa
     5941 aatatagcca tcagaagtta cgttaaattt aataacgata cgttgccaat ccgtgtttaa
     6001 agtgatcgac ttgaaactaa tacttatctt agcctgttgc gattgatcat ttggtaacaa
     6061 atataaatta acaacatcat tatctatgct tgatttagca tatgcactaa aaacgtaatt
     6121 ctctccgctt tttacagcat acaattgtga tataccactc cacattgctt tgcgctttag
     6181 cactgttaag ctaaaattac caatagtcaa gttatcgtca tcagattgcc agttattacg
     6241 attaaaccaa tttttagtcc aatttttagt accagatagt agattaactg tacctatctt
     6301 taaattatca atagtattat taacacgagt tatttctctg ctaaaactat ctgcagtttc
     6361 cttgacttta ttatcagcga taacagcaga cttacttgta atcaacgctt caatatttgt
     6421 ttgagataat ctcgttgtaa tttcattagc cgtttgtgtg acttgggtct gtagactgct
     6481 aagcgtacca tcaacagtct ttttgttagc agtaatactt gtactatttt gatcaatcgt
     6541 ctgcttataa ttagcgagtg tttgagtgat atctccatct ttttttaaca atgcctcgta
     6601 gctagtttgt aatcctttga ctgtaccatc aagttgtgtc agttgcagat tagttgttga
     6661 ataatgctca acttctgttt tagataacgc agacatttta tccgtcagtt ttttagattc
     6721 actaataagg tttgtcaatg cttctgtagc atcttttttg gcagaatcag acaaattctt
     6781 tgcttgatta attaattcgt cttggctaac atttttagca acaattgcat tatatttttc
     6841 agttaactgt tcatcagcaa gtttcattgc tgcattaacc tctatgattt tattagtaat
     6901 ttctttcttt acaaaatcaa tatcgtcgct aaaagaattg ataatccatt cagcaccatt
     6961 ccagaaatac attctggtag tatcgccaac agttaaaaaa agcaaatcac cctttcttag
     7021 cgtgcctttt ggatcatcta ctggcatctc cgtaccatag tagttagtgt ttttaccatc
     7081 agctgatgct agtgcttgat ttgctttaaa tactgcatta tctagtattt cttggttatt
     7141 attaatctta cttgataatt ggctaacagc tacagtttgt tttttgattg taccaatatc
     7201 gttacaagtg actttatggt taatcagctg acctgttaca tcatactcac tttcaaacga
     7261 tactattcgg attttttcct taaatcctag tgtctcattg atagccataa tataatcacc
     7321 ggcaataggt tgagtgtact gataacctgc ttttgtaaga tcttcaatgt caatttttac
     7381 agcgatattg taagacgaat ctacattttg ttttagtact gattttaaat tgtcagcgtt
     7441 agtgtaccgc tcatcaacaa caggctcacc ctcaattcgt ccatattcct ttgcaagtgg
     7501 actttcatat tctacttcga gccttccttt ggaatgatcc tcttggtcag tccacgctcc
     7561 aaaacccttc tgatatgtaa taaaatcatt gattttcttt tcaattatga tttcattcat
     7621 attgaaattt ttacgtacga ttgttgataa atctgttcct gttttttgta gaattcgaac
     7681 aaccttgcca cgaacaaaaa attcaactcc tgcactctta atgatgtcat taaataaatc
     7741 aagtcgcgat ttatatccaa acgattgctt ttcgaaagat ttgacaacta cttctaaact
     7801 gtaagtatac ccgctgccat taaaaataaa gttcaaatat gcttggaaag tatgtgaacc
     7861 atcatttaat tgcttatgta gtgatgattt atcaaaatca taaaaaaatt gatgaactgc
     7921 atcaaattca acttgaattt tgttaccagt atcagtaggt ttagcataaa taatcttgta
     7981 atactcatca tcaaactcaa gtttccaacc tttatcgacc ttgtttagta cctcttcgtt
     8041 agtataaatt gttcctgata aagacctttc tccgttaact gcattagttg ttttaacaac
     8101 agcttgagct tcaaactcat tatctttatg atctaaaaaa gttgtcaatc tatcacttcc
     8161 ttacttgtaa agttctttaa agtttaaaaa cttgattgtg ccttgaaaat ttgtgctgta
     8221 gagaatagtc ttgttgactt tttgctttaa aacaaaatat tcatgattgg ttctagcagt
     8281 cacatttatc ttattcaatg ttgtggatat accagtgatt acaataacgt cgccttgatt
     8341 aagtggtgca ctatattgat aagcccagcg cctcccatcg atttcaagat aaaaactact
     8401 ttgtccacca gttgaagtta actctacaga ccatgggtat tctaactggc taaaaggtgc
     8461 agtaccagaa taattaaacg ttccgttagt aatgactata ttttgtgcaa tagtttcacc
     8521 aaatggaagt tctgcagtct caaatgcaat tgtaaagttg tatttgagac cttcactaga
     8581 cttaccaaca aaagtgtact caggttcact tgagacataa actttccaac gatagtgcca
     8641 cgctgtatga gattgattta ataagtctaa atcacctgct ttcatcccag gcaattcgaa
     8701 atcatataaa ttttctttct gaggatacat tttagtaatg taaaaaggct cactatccac
     8761 tagtaagcca ttcacatcat ctttttttgt cataaaatct ttaatagaag ctacaactaa
     8821 gcgcccatta accttaactt tttttgtgcc atacttagca ctagcaaaga ttttaccgct
     8881 tcgacctttc actgttcgac tatcaacctc aagagatgat gaacggtcat cgacatcaag
     8941 tattcgtaga ccaatctctg aaaatcgaaa ggattgatca ctcttttgta ctaataaatc
     9001 catttttctc ctctcttatg tcgcaaagtt gaagtaatta tctttattac tttctcttgc
     9061 ttctttagat ttaatagttg tataaatttc atcaccaaca agttcattat gaacctcgaa
     9121 cgttggacta gtcaattgtt ggttgcgtac ctcgtcgctt agattatcaa gtgatgatga
     9181 tacacctgca cttgatatgt ttgcagatgt cgctaattgt gaatctgttt cccatcgctg
     9241 atcagtcaca gcattagcgt actctttacc tattacattg atatcatcca tccaatcctg
     9301 cataccaagt gcaaaacctt caccagtata agcaccaagt tctcgcgtta ctcgcgaagg
     9361 cgaatgaata tccattgcgc ttctgattgt tgcagcgaca ttagcagcaa tactattagc
     9421 aatagccata atagttcctg cagtgcttgc taggccattt gcaaatccaa tcccagtatt
     9481 ataacctgca gatctagcac cactcgctgc tgcattcatt gcagatacaa ccgaattcat
     9541 tgcacttctt gttacactca cagcgctgtt cattccactt gaaattgacg aacgaatact
     9601 agacatagca tttgaaactg tagacttagc agaattaaag gaacttgtaa aggtggattt
     9661 aatattattt ccacctgatt ttacagtatt attaatacta ttcattccac tagtaacagt
     9721 gctgttcatt cctgacattg aacttgaaac tgtagattta gcttgtgtaa aactgctatt
     9781 cacactgtta gatatatttg caccgcctga ttttgcagca tttgcagctt gattcatccc
     9841 tgtagataca gtgctagtta tacctgacat gttattagct acaccactag ccataccagc
     9901 tgtagcacca gtaactccag aactcattcc attcgcggaa gtggtagcat ttgcttgtgc
     9961 agtttgcata ttacttgttg cctgttgcga taattccaag aaattcggta gagaccccag
    10021 actcatttgg ctagtagcgt tattaacatt agcagtcatt tgattagctt gattagtagc
    10081 atttgtattt gctaaagtca tgttcgaact caacgcttca ttaaatcctg ccgcattcag
    10141 caaaccttga tttgccatag atgctgtttg tgcatttaca tttgttgcca tctgagtagc
    10201 atttgttgtc gcatttaaat tagcaagacc agtattttga ctaatgctat ttagcatagt
    10261 agttgtgtct atgttagttt gattagccat tgtggtcgtc tgcataccaa cattcatagt
    10321 cattgttgac atgtctgatg taactttagc ggcacttgtt gagctctttc ctgtgatttg
    10381 gtcccacata gaactaaatc cacctttaat gccttcccat acccctgaaa gagcattcgg
    10441 aatagcctca agcatagctt tgccaagtcc tataatcaat tgaataccag ctgaaataat
    10501 ctgtggcaac cctttaataa ttgcaacagc caactgcaca actaactgta gccctgcaga
    10561 tataatttgt ggtaatgctt gtgataaacc agatatcaat gactgaataa tctgcatcgc
    10621 tgcttggatt acctgtggta aattttgaat aataccttgc actaacatga ttataatctg
    10681 gatgccgcct tggattacct gtggcaaata ctgagctata ccggaaataa aacctgtgat
    10741 gacttgggtt gcaatttgta caactgcggg taacatttgg ataatgcctt gcactaaatt
    10801 agttaagata gcaatgccat tattaacaat gcttggcatg tttgcttgta agcttgcccc
    10861 aaaattgtta ataatttgtt gagcatattg aataattagt ggaatatttt gcacaattcc
    10921 ttgaactaaa ttaactatca attccatacc aactgctaaa agctgaggta atgcacttgc
    10981 aattgaacta ataaatgtcc ctataatttg tattgctgaa ctaattaaac tcgtagcatt
    11041 ttgtccaaca ccttgaacta aactagatat taattggaca cctgcttgaa taataacagg
    11101 taacaataca gtaatagcat ttgcaaattt agatataagt tcagcaccac tagcaattaa
    11161 ttgaggtatt tgtgatgtaa tgccatttac caaattagtg ataatttgag aacctctagt
    11221 aattgctata tttagcaatt tatcaatttg agtaccaaat tgattgttta ctaaaccaag
    11281 acctgctacg accaatccta aaattgccgc tggccctatt gctgccatag caacgcttac
    11341 cacagctccc atggcttgtg tcattgcacc gagtatagca acacctctcg ctgccgcagt
    11401 tgcaaaaatg gcaggtaaac cacttactgt tgctgcaaaa gcaccaatta ttccgctagc
    11461 agttgacaat ccactggcta ccattgctcc gaatgatccc aacgctgaac caacagtccc
    11521 aagagcaaca ccaatcttac caataattga tattaaaggc ataatagttg caatagcact
    11581 tgcaggattt aataaggccc aagctgctat tgcagtagga cctatagatg ctatctttga
    11641 tttgattcta tcaatagttg ctgacgatac ttcaccagtt tttgcaaatt cacctaaagc
    11701 agtatttaat aatccaaaag ctgatttaat tccactagtt agtggactaa agtcaatatt
    11761 caccatcttg gagattctag cgaatgctgc aacaaggttt ggcattattg agttcacagt
    11821 tgcaaatgca gaatttatag ctggcttaat actattaatg gattctgcaa tggtcttcat
    11881 accattagct ttggcagctt catcaaaggc tgaaatcata cctgctacgc cttttacgac
    11941 ggcggtatgt acgttcttcc atgcagttcg gattccacca gccgatgatt gggccatctc
    12001 agcaaaacca ccctgcgcct tgtttagttc aatcatttta tcagcgaatt cttgggcagt
    12061 aatactgccg tctgaaagag ctgcttttaa gtcctgaaca ccatttttac caaatccgaa
    12121 tgactctgcc attttagaca ttaagccagg ggcagcttca gacacagaat taaactcttc
    12181 ggcatatatc ttaccagacc ccaacgactg gttaaactgt ctaagtgctt gctcagcccc
    12241 ttcagtagtc gctccataac caatcattgc attattaaat gctaatgcta gctctgttcc
    12301 tttatctaag ctacctgtag ttatagctaa ttgttgggca cttttaacag cactgtctag
    12361 tggtgtagga agtccttcaa ttcctttaga taatttatct attgatgctt tagactgttg
    12421 tgcagaatac ccaaatagcg ccatcgtttt cggaaagcgg ttcatggtat ctacacgact
    12481 tattgcaccg tccattgctc ctgtgattgc ttggattcct ttctgagcaa tggccatcaa
    12541 actaaaagca gaaatgatac ttcccacagt gcttcttagt ttttcaccag cagatgatgc
    12601 gcttgcgaat ctctgactaa taccatttag ggcactagtt gcagaactgg acatcctgct
    12661 aaaactattg ctaagacctg tgcttaactt agtcgctaaa ttagtaaccg ctgaagtgat
    12721 tctaccccca aaactactac tgattttatc tgcaacagta ctcgctttag aactaacaga
    12781 gttgaaagca gctgagacgg cacttgtgat cttagacgaa aagctaacta ctgcacttgt
    12841 tgcacttgtg aatgcggatt ttatgggttg agggatggcg ctcgataact tctgtacgct
    12901 attttgaata attgaaaaag ctttattgaa cccattcttt atgggttcgg gaattttttc
    12961 accgatagat gctgctatcc tttgaatttg accaagagat aacttgagac ctgtactata
    13021 tgcattaccg agtctttgcc ctaatgattg accattattt gctaattgag ccataatttg
    13081 accaatcatc tgtatcattt tgttgctgtt gttaactgca gcatcctgcg cttttctaaa
    13141 agcattttgt gtagtactaa caattctagc catagctgcc tcataatctt tagtatttgc
    13201 accaatatca gcgaatatag ccccatcaaa tgtacctgcc attaaatccc tcctttcttg
    13261 ttatatcttg ttataaattt tgaaaatgta aattgagctt cgctacacgt tctgcgaaat
    13321 cgtttgaaat attagttaga tcttgtttat tttgataagt tcgtttaatt ttgttacgtt
    13381 gtttatctaa tttgagctta ttagcattga cgtttttagc atttaacgtg tatctgagat
    13441 ttaaagcaag ttcagagagg ttttcacgtt ctgcaatttg cttgtactgc aaaccttcca
    13501 taatcgcatc taattccgtt cttgtacact tgtaaataat atcaatgtcc gtcaggccaa
    13561 gttgagcaca attaattaag agattgcgtc tttcattttc ccaatcatgt cttttacgat
    13621 ttggatttga gcttcgtccg catcttctcg agcttctaaa tatttcactg atttctccat
    13681 gctttcgata tatttcgaaa tcttctcttt gaaaaaacca gattcaacca tgtcattctc
    13741 aatttcttta aataaggctt ctgtcgaccc ttcttcttcg actagctctg cgatagaatc
    13801 aagcacttca tcttctgaca atgctttttt accgctgcct acagacagct tgactaaatc
    13861 tactacagct gaatcatcac gctctaagat tttataaaat agcgtaccga cgccgtcagc
    13921 gttacgttcc cctgtctctt tattcacagt cccaagtcta ttatttatct taaacattgt
    13981 tttaaaatca aatttaattt caatactgcg ttttgctact gttaattcca tttatgtatt
    14041 tctccttaaa aataaaaggt agcgaaatgc taccttataa attagtgtcc ttctgataat
    14101 ttttcaggaa ctgctcccgg tgaacttcct gaaacttctt ttttctggat attgtcataa
    14161 tcgccagtag tttcgcctgg gttttgatat gcataaacac ttgttagcaa agcgatatct
    14221 tctgccgtca gtggaaattt gccatctttt aaactatcga taatattcaa tgcatatgat
    14281 gcttctacaa gatcttcaac accttctgaa tattcaatct cgtcaatttt tccatatcca
    14341 aacttagcag gataaacttc tctatctttt aaagttcctg aacctttttc tttcaaagat
    14401 tcgtctgcga taacacgcca aactttgaca gatttaccag ttgttttagc tatttctaca
    14461 atttcaattg ctttgtcttt cggtgtgtag tatgatgtca aatcaattga gtgctcatct
    14521 gttgcttttt caagcacacg tccttgttga gtttgttcat caataaaatc tccaccgagc
    14581 gtcgttgtcc catctgtgcg atacgctggc agaattgctt tactacctaa ttcagcatca
    14641 acagattgaa taaaataaaa tatattttta cctttaatag gcttagctgt agtaatttca
    14701 actgtagttt ccatttaatt ctcctttaaa ttactatttc tgtgatattg atagcaatat
    14761 ggtagacctc acgacctaca gaattatcca tcaataactg tgcattaatc cgtttgtttc
    14821 gtcctaagga cataatcatt ctagacttaa cctcctctgc atctgttctg ctagtggcag
    14881 gtaagaatgc gtcaatattt agcccaaaat ctcccacaac agctcctgtc tgcgctgttt
    14941 ttgatgtatc cgatacattg gaacctatga caataaacgg ctctaaaacg tccgattttg
    15001 gtaatttaaa atagattggg atattaagtg ttcccaatct attttttacc tcattcagat
    15061 ataaagttga tggtgagtat atcattgatc acctcttaaa catttttttg aggttagcca
    15121 ttaacactgg ccattctttt ctcagggcag ggtctaaaaa tggttgagct tccattttac
    15181 gagtacctaa ctcaagataa atagaatata aaacaggaga aaccactcta tagtgcaaga
    15241 gtttttgttg ttcattgtag atttgacttc tcagccatgt agtatcaacc ggggctagta
    15301 ttttagcctg tctctcaatc ctagctgctg atttgctaag ttttttatct acagcgattc
    15361 gtgctgactt ctgcttgcgc tcaacactgc gtaaaaacct gtccagtcct cgcatacgat
    15421 aagtaagact catacataaa taaccgtgct atttttatgg tgcgctagtc cctttatcaa
    15481 cctcggtttc cccttgtaga taatcttgct aaagccatcg taatggcctt gcaagtgcaa
    15541 cttaaaagcg ttcaaatcat atttaccaaa taaccccatc tgttcagatt ttgtgaaagt
    15601 attttccatg caggggattt ttccgctagg tttttcaaca gttcttttat ctagaaaatc
    15661 gtcctgctca tcgacataca ctaagataac tctatctttg taaatcattt atgacctcct
    15721 aaataaatct agcgacacct tttccacggc accttttacc ataagcaaca tttctaagaa
    15781 ttgagtcata ttctgtgaga tagtcttccc aactaaaaga ccgaccttct tcagaatctg
    15841 ccttggctcc ttctgaatta agccgattat atcttttaat agctacatct cttgcgataa
    15901 atgttaaacg tcgtggtatt tctaacagct cttcatcaga atactcattg agtttcgcaa
    15961 ggatacgttc aatactttcg tcaatggcaa gtttaatcaa ttcgtcttgt gaggcatcat
    16021 tcttagcaat acctttaaaa atttttacct caactaaaag tagttcctta tccatttatt
    16081 ttaccctcct ggaactgctc ctgggacctc tttatctgct tcaatagttg cctctacaac
    16141 tccttcagga atttcagcaa atagtacatt cgctccaaaa aatactgact cataagttaa
    16201 gttagataat tgacgttgac gagctgcagc aatcaagcca gtctcatctg tgtagtctgc
    16261 aaatagacca cctaaatctc caccacgtac attcaacgaa gcaaaaacaa gattatcaac
    16321 tgcagttgag taaacttttc cttctggcac agatggcata acgataacat tctgcatccc
    16381 taaaaagttc tttagtaacg tcatcccaaa aacattagaa gcatctgcac ctactttagt
    16441 atcaccaagg taatttgcta catctaaagg agatacaaaa gaaacaagtt gagacccctc
    16501 aaactcatta aaagtagcta gttttgccca tgataaagat agtgccttct gaagaccagc
    16561 ccctttaact tttgttggtt ttgttttgag gaatgtaaag aacgcatcct taattccatt
    16621 ctgaagctca cgcataatgc gcttatctgc ttcagtgata gcacgagctg caccatggcg
    16681 agcaattgcc tcagctgtag ttgcacgacg tttcttgaac cacttcactg tatagtcttt
    16741 atctttagtt cgagtaacct tagacagagg gattgtttcc ccttctccag gatcagtttg
    16801 atctaaagtc acttcccact tataggtctg aatttttaaa tcactggtta actcttcacg
    16861 acgagtaacc cctagtagtt tcagtaagtc attaatgttt ttggaaaact tgttaacaaa
    16921 atcaatcgat ttaatgtctc ccaagtctgc catggtgttt aaattttttt cagccataat
    16981 attcctttct tattcttaaa ataagttacg attttcagca attaaacgca tacgttcgtc
    17041 gtcgtcttct actgccataa tctcagcttt agtcatgccg acagagccgt tgccagtccg
    17101 tggtggcttc tgagtcaaac gattattaac gcgcttttca acagccttat caaaaataat
    17161 ttgtaaatta tcaatattag tcttaacttc ttctgcagta gtacctaaaa caacatcaat
    17221 aaattctaat ggcaaacctt tttccacaag gagcgtttga gtctcaatac gcatttcacg
    17281 ttctgcgact gctttttctc gttctgcgat tgcatcaaga cgtttttgtt cttcttcctt
    17341 agcacgttca tccttggaca ttttggccaa tcgttcgccc tcagtacgtg ccttttcaat
    17401 agcgtcagat tgttctgctt cccatttagc tcgctcagca gtcatcattt ttgaaatatc
    17461 cgcacgagta aatgtacgct catgttcttg cttagcttgt tcaacagctt ctttctgagt
    17521 gtcgacttgc tcagtagttt ctgttgctac attttcttct gccataattt tctcctttag
    17581 tggttacgcc accaaccgat agtctagttt tacgtcctgc gacgaaacag tgtagctttt
    17641 agtgtcatca acaagtttgg acataataaa aagccgtatc gctacgacta atctaaatga
    17701 ttagtaatta tttcttccat tttttcttat agttattctt tatataatca agttcgttat
    17761 ttgtcgcttg agcattatgt tctacaattt gttcaagttg ttcaattctt ttatgttgat
    17821 ttctaagagc ttctgcttgc atggcattct ctgcgataag catcacaata gctcgttcga
    17881 gtttacgttt cttcttaatt cgtttattca ttgctttatc cttccttctt cacttctgca
    17941 ataattgact taactgctat tgctgcaatg ccaataatca atatgagcac caatacaccc
    18001 agtgctacta ataccattcg ccaaataaac atatcttcct ccttgataat tctgctatac
    18061 tggtgtatac ttatggtagt gaaagacggt ggtctaacgc ccatgaagca gttttctgtg
    18121 gaggcggtgg gtcatcgtct ttctttttct actacagcta acacctcgtc tttgtttttg
    18181 ataattgata tattgagacc cctgcgatct atcttataaa tatgttctaa ctgccccagt
    18241 atttcttcaa aagatagtgg ggtttttgtg atatcaaaaa tgatattagg tgcttgcttt
    18301 tttgcttttc gtagattacc atcgatgacg tttttacctg aacctgtgat ttctttcaag
    18361 tcaaatggaa cgtcgtcaac taaatagtct ggagtgttaa tattttcggg gaaattaacc
    18421 ctcggaacca tttcaacttg taatcctgtc ttcttagata accagttagc aactctatat
    18481 tcgtagtttg aatggtcaag tactacgtta tgaccatcaa ctctgtaagt tccgcctcct
    18541 ttagtgtact cattcagcat agatatttga gactcatact cttgcccatc tatccaatct
    18601 gatgtgacat ccttaaaata cttagtactt ttatcagcga taggttcaac atcggtatta
    18661 agttcatcct cgtcaggaat aattgtagtc ctacaattaa agtgatatgg agcagcatta
    18721 attccgggct gtatttcaga taagagtttc tttttatttt ctcttgcctt acttcgacat
    18781 atctgagtag ttctatcatc tagctgaact aagtctcgat aatacttaat acctgcatca
    18841 atgtatcgct tggctgtagc attattgata atcatcgtgc catcagtacg aataagcgtt
    18901 tcagccctat gtctggctac gtcgtaacgt ttgcccaatt cacgagccat ttctctagga
    18961 ccaagacctc tgacaaatcc ttttttgaat gcttcttgta aatcttttac tagattatca
    19021 acatttcccc agagttgctc tgagtaatta tagccgtcaa atggtgtctt gattaactgt
    19081 tctagagctg gcccattaat tgttcctgag cgaccaccta cagatttttg gtaagcataa
    19141 ctagctgcct ttcttagatg atcgctaaac aaaccatcta agatacctct catagtacct
    19201 gcacgatgta taagctctaa attaagcatg tcaagacgag tagcctgcgc tgatacatat
    19261 tgttcattaa tacgtttaag tagctctggg tctttctcag cttgctctct atatcgattg
    19321 gcatttgctg cataatcact taaatcttcg tgtctaagct tcttaacagc atcttgataa
    19381 gttatcttat tatcttcagc aaaacgatta taaaaatcat ataaatcctt ttgtaaagac
    19441 aatgattggt tactatatac tctagataat tcattgaaaa tatctaagtc tgctctatct
    19501 aaataactaa caatatcttc ttgacgtttt tgccagtaat caagatggtc ctgctgttgg
    19561 ttcttcttgt ccattagcat cacctacatt tcttggttct ggcattactt gcttctcgtc
    19621 agcctcttct ttcagacgtt ttaattcagc ttcagcatcg acaccagtta ctgcgtttaa
    19681 aatttcaaaa atagtttgat cgctaacaat tccataaaga ctctgtgcag ccgaaacaat
    19741 ttcattatca ttctgcggca cattaggagt aaatactata ctagtatcat taacttgact
    19801 atataccgtc gattcgttcc ccttgattga ccagatattg gcagctaaac gtaaacgtct
    19861 catgagtcct tttttgaaca accgttcttg cttttcacgg taattatctg aagccattag
    19921 cttatacttc attgactcgc cagattgaac accagagaat tttaaatctt gaatatcggg
    19981 agtaaaagta aagcgtaaga tatctgacac tagtctgttt ttatacgctt cacttcctgc
    20041 catatcatat tcttttttta gataatacgc atctggctta gcaccatccg ggttaggatt
    20101 gtcatcaagg acaataactt ttgccttctt gaatccaata gatataccta aacgaccgtt
    20161 tggatttata cgtccatctt caaaaaaatc attttcgtct gctcctgtat acggattacc
    20221 agagagaact aggattgcat ctacagaatc ttgttgaaag ttagcaagct ctgactgtga
    20281 taagtcgtat gcatcaatat tatctaagac tgattcataa gcgcctgctc tatcatcatt
    20341 attagaatat tcattaactg gaacaccact aaagtaatgt tcttcttcat ctttcagact
    20401 cattcctttt gtttcttgat taaagtcctc gtaagtaaat atcatatttg aagtatagac
    20461 cttaacaatt tgcttacgtt tacctgcgcc gtaatcgatg tcgtagaaat gtacagccat
    20521 caaagaatta tgttgataag tatcatcata aataacaaat gtctgttctg cgggtagttg
    20581 atagagttta atttctgtcc cattatctac ttcttcaacg gttaaaagct cgtaagcacg
    20641 accgtaaatg gataagtccg ttttgattgc tacattgtga tagtcttcgt tgttacgaac
    20701 actcataaga ttaatgtttt tttgaatatc actatcttcg ttcttgtact cgacaggcac
    20761 accaagcata taaccttgct caaagactgt aatgtactta gcaaaatcac tagcaatacg
    20821 attgtcagct gcatattcat cggtctttgc tggacgatat ttgatattgt tatcaccaag
    20881 ataatatcgc tttaactctt tcaatcggtc tagttgctca gtctcaaaac tagaaatata
    20941 tttttttaac tgatcaatcc atttctgcga attatattca atagcatcaa aatcttcttg
    21001 caacatgata aattgcctgt tcgcattttc attaaatctt gttcctgata aaaattttct
    21061 ttcaaacaag ttcaaatccc tctctatcta aaataatatt tagcattatt tagtcgttta
    21121 tttctgtcag atgattgata tattctatct tgtactgcat atctgatagc atcaatgcaa
    21181 tggttatagc tgtcaactgg ctcattgata tactcgttag ttttcttatc ttttttccaa
    21241 gtgtaattct ccagctcttc aattgtttta acacaccttt cgtcaacaac gatatcgtac
    21301 tgtagcatgt actgaattcc ttgcataact gaaccagctc ctttttttac atcaatcatt
    21361 ctttgaatac ctaaatttct caactcttgg ttagatttct tttcagctga atcccctctg
    21421 atttcttctt tggcatatcc gaggctcttt atagcattag ctattttatc attggtcaaa
    21481 ttctttctga catattcctc caagatgtat agcttcttgt ttgcatcatc gattttgaca
    21541 tgcaaaaatg ccgaaggatc attaatgaat ccgtagtcca aaccaaaaaa agaaggcaag
    21601 tgtgataact tgtctttgtt tagtattctt ttttcatact ttggaaatat gagcttttct
    21661 aaagtcgcga actgacctag agcataaatc ttgtaataag cttcgtttct gtttgctagt
    21721 tcctctatat tttctctagt aacatcatct aaaaacctat tatctttata tgtagtttga
    21781 taaactacag tgtttttagg tgacttgata aagaaagctt tataaaccca gtttgcttta
    21841 ctcacaggat tgaacatgag atagacttgc ttctctagat gtttcttatc ccttaaacgc
    21901 aaagtcaact gtgtgtaatc atcaagtgta aactcactag cttcttccat tacaacatca
    21961 gatatccctt taattgactt aatcttttct ggattatcca tgcctttgaa aataaattcc
    22021 gagccattcg gcaatgttat tctaaaagct gacatattta tcttgcattt atccaagacg
    22081 ccaaaatacg acaagttaga cataatatca gcaaatacag agtctctgac agttgcacca
    22141 acttttcgaa ggacaagaat ctttctcgga tgtttaaact ttggattgag tgcttttaag
    22201 ataatttttt ggaatacacc gtgtgatttt ccagaagatg cacctccata gtgtacctct
    22261 gtgaagttat cgtaattata tagtttatcg tagatatgtt tattaaagac tttgctagga
    22321 tacttgatga caatattaat tttagggcgt tgcttagtcg tcatcccact cacctacact
    22381 gatggttaca tctgaactta tatccatttc aattttgtca gtgaataatc tgtaccgctt
    22441 acctagtagc tcagctgcct tgattctatc ttttgcacct acatcaatat cgacaatctc
    22501 ttgtcctaac tccccgatgc tgatcagtgt tttttcttgc tgatcgcctc gcatgattga
    22561 tgtaaggtat tgtaaaactt cttcttgcgt ggcaatcttt gcagattcaa gcttttcaat
    22621 cctttcatct atataagctt taatgtcagg tttggtcaag ttttcttgac ctattgacct
    22681 cgctgtcttt ttactgtaac ctgctttaat agccgctgct gtcgcgttag ctgagatgat
    22741 gtactcatct gcaaaacgct tttgttttag tgttaattta ctcaattttc catcacctcc
    22801 ttcgtgacat aataaaaaga caagacacta tttgccttgc cttcaaactc atactaccaa
    22861 tttatcatca aaaaagtgag aaaaccaccc ttttttgtct caaacttaca attttccaat
    22921 ttgttgtgca aatagagtca aaattctatc cctttttcgg taggttcctg ctctagacgt
    22981 atgaatctta taagctatct cctcccaggt attattagaa ccagttcccc accttagatc
    23041 aaaaatataa ttcaattcat cgtcaagcag tgctttaacc ctatcaacac tttctctgaa
    23101 gttttcataa cttttgattt taccgtcctt tgaccacttt atgacaatat cttctgtctc
    23161 tttagctact agattagact tccccccgcc tacattgata tctgtactaa caggcgtctg
    23221 cagctcaagt tttctaattg caattttacg ttcaatatcg cgataatcaa acaaccactc
    23281 atcaaaagcc tttaactgtg cgttagataa tttactcatt ccgtcacctc ttctctaaac
    23341 tgccgataat aaacggtaaa aataatatta aaagtatcaa aacaactgct gtgttatcat
    23401 ccattcttat ctcctgttct ccaaaaaata aataaatctc tacttagtac aactggttta
    23461 ccaaagattt tatatgatga ctcaagattt tttatttcaa catcaaaacc atcaccaagt
    23521 cggtatttta gtaaatctat tgttttttgg ctgtcaagtc gtctagctaa gtattcatca
    23581 ttttttggaa tagagatttt ataaccagag aatcctttca tcgcagactg tttaagtttc
    23641 tcctcgattt ttaaaccatc aaagtaccta tcaaaccatt ttttgtgaga ttctgaaccg
    23701 cattctttta cttcatcaat taatgacaac tttatccccc atttcctgtc aactccgcaa
    23761 tccgctttgt ctgtctctga ttttgctctg acgactcttt caaacggtac tgcgtgcgta
    23821 ttagttgctg ttgtaaacct gtgatttgtg gttggtagta gttgtacaat gcaatatttg
    23881 ttgttaacaa cacgctgact gctacagtta atacaacaat cacaattgac tgtttgtgta
    23941 atttgttgtt ttgttcgttc attcgttcac actttctagt aaccaatatt caacgagacg
    24001 ttttgtatat tcgagtgctt tttctaaatc ctgagtcgca ttgcctttgt atctatgtcg
    24061 tttaatgtat ttatcaatgt gactttccat gattgcgaca aactggtccc atgggtatgt
    24121 cttataccac gactcaatca aatcaatttc cccatgatta taatgctttg gtttatgtac
    24181 taattcacct gtcattttgt tttcctttct agccactccc aaatcatgtg gaattggcca
    24241 ttcaccaaat cgtcgttgcc ataactctcg caaatatcaa cgattgacat attaacccat
    24301 acccaatatt gttcagttcc aaaaccaagc tcctgcattt ttttgttact ttctctcatc
    24361 cagccaggaa ccactcgttc aaaaaaatca atataatcaa ttttcataac tcttccacct
    24421 tcacatatat tcctactgta tcagcccaga acttctcaat aatttccgaa gcaacctgcg
    24481 catcatcatt ccaaaatccc aattcagtca tgcaatcttt aggtaacttg ttcaaattat
    24541 ctgtgtcggg tttagtatct ttatattgtc cgtttactga ttttttgatt ttcggaaata
    24601 accacttggt tgttagtctt attggaccat ttaatttttc tttagggaca tgttgagcaa
    24661 acttatccat aaacattgcc ctagcttcct ttagtgaatc tggttcatag aattgtggtt
    24721 tcccatttac cacacggacc tttttttgct gatgtgttgt ggttggtatt tttttcattg
    24781 ggataaaaaa ttcaattgtc atgatcaaca ttatccaagt ttattttttc aaattgctgc
    24841 acgttgttct aaaattttta gataagctcc cattgcatgc cattgtgcta ctaataattc
    24901 aataggacat tgtaatttaa aatctagact atcaatataa aatttattaa ttaatactga
    24961 taatttttct gcacggcttt ttaattcttg atatttaaca atcattcttt ttttgtattc
    25021 tttcattttt gtcacttccc atcttttttt attttcgcgc atagtcaatg tcaagggaca
    25081 tggttacagg gttacaaggg gcggatgcat agcccccttg ttcctgttca tgtacccatt
    25141 gaccaattgg gacatttcct aaatattctt ctttcggaga aagagaatat tctgtccctc
    25201 aattttgtcc ctcaaaagtc aaggacattt tcgagaaatt ttcgagtttg tccctatttt
    25261 tgtccctgaa aatcatgtaa tagggacacg gacattttcg agtttgtccc tatttttcag
    25321 ggacattttc gagggacact ttcgaatttg accctcgatt ttgtctgtcc ctgtccctgt
    25381 ccctctgtga aatattcaca aacttttatt ggttttaggt tcttctattg gtaaaatttg
    25441 tttattttta acttcaaatt taccgttttc ttttatccat ctacgaatag ttttttcaga
    25501 aactggctta tcttcagtag aaaaatattc gattaagtta tcgattgtga ctggctctat
    25561 tccatcattc aatgaattaa ttgcagtctc tactttttga gatttctttt ccctggactc
    25621 tcttggactc ttcttctcaa aattcttctt ccaagtcggc acattatctt ctagtgatat
    25681 atcagccaag acacccgttg tgtctacatc atgcactgga tagctaaacc acatgttaac
    25741 tggttggaat ttagcaaact cgcgtaaagt cccttcaact cgccaggcag tggaaatctc
    25801 cacctcatac tgggctttct tgaccgcttc cagatagggc tgtttaatca tgatgtcctt
    25861 gatagccttg tcaaaatgtt gctgcatctg ataacgactt tccaaatcat ctagcgttac
    25921 ttcctgttga tagtaggcta gcgctttttc ttgcaaggct tgttggtaaa tcttagcggc
    25981 tgctttttct gagcgtgact tgatgagttc ctcagtcaat tctagctcta ctaggtcaat
    26041 cagtgcatca gggtcacggg caaacactcc tgaaccactt gcacggtcca tagatttttt
    26101 accgccttgg ctcccctttg agtgatggtg gcagtaaatc acactacaac ctaactcagt
    26161 agctactttg tcaaactgat tggtaaagtg agccatttgg tccgcactat tttcatcacc
    26221 agtcagcacc ttatagatag ggtcaatgat gaccgcttgg taatttttct ttaagctacg
    26281 acggataagc ttaggcgcta acttgtccat gggaacggtc tttccacgta ggttccaaat
    26341 atcaatattc ttgatgtttg ctggtggcaa gctcatagct tcgtatacat cacggaagcg
    26401 atgcaaagct gacggtctat ccagttccag attgacatac aagaccttac cttgttcaca
    26461 ctgccagcct aaccattctt taccttcagc cagcgcgatg gatagctcaa ttaatgcaaa
    26521 cgacttacca gcttttgatg gtccagcaat caacatcttg tggccttgac gcaaaactcc
    26581 tttgatgagt tccggtgcca attctggcat attatcccat tcgtcagcta gcgtctctgg
    26641 gtcaggcaaa tcgtcattta aatcttcaat ccattgatac cattcttcgt agttggtttt
    26701 acccacatta gtatcaatca agaattgctt gtgcccattc cgagtcacac caggcatgcg
    26761 tgataatcga ctagggttgc ggttctgtgt gtcaatatca agtccattct ttttacaaat
    26821 ttgatagata taatcaaccc gtttgcggta ctcctgataa tcacgagcat ctactttgac
    26881 aatagcgtgt aatgattttt taccgctatg gactaaggtt gctattggca attctagttc
    26941 tttaaacaaa gcatactgtt taccaagttc catgctgtcg gattcaacca aggcatatct
    27001 aaagtctgtt acattgtcat tcttgacacc atttccatct agtggattaa aacgaatcca
    27061 tgcaccagcc tcttctttga agtctccaaa tactgctcct aagtcagtag gatttttttg
    27121 gagtgattca atcagttgac cagcagtcct gtcatacgct ccttttgttg gtttataaac
    27181 aatctctccc gtatcaaggg taattggata ggtctctgtt acataaccaa ctttgtcagt
    27241 tgattcaaat aaggtatcta tgtaagtaat taaatcttga actggattcc agtttcttgg
    27301 ctcttgaatc tctttcgatt caatccaatt tttatcgaca atcttataat cacgatcgat
    27361 tgtatcgtcc cagctgagtt catgagcatc atcgctgtta cgatactctg atgatgtcca
    27421 tccattatct ttagctagct gtgtgattgt tgcccctgtg atagtcccaa agccattgcc
    27481 ttggaaacta tcccattttg caaaacactc acctttttta tatctactat cagattgtga
    27541 ccaaacatcc caatccattg ctgtataacc ttcatgcttt agggccattc ctacttgaac
    27601 ccatgtttga tagtctaccg tggcaggatt gatataatcc agcaacggta gcaaattaaa
    27661 atcattctct gccatgttat cctttctgca catgttatcc tttctgcatt tgatataatt
    27721 aacctaaaga aaggaggtca actatatgcc tgaaataaaa attacttttt cagatggttc
    27781 tattgttata tttcacgaag aacagagttt tcaaagtgta gtaaaatcaa atgatgctat
    27841 gtccttatca aaaatatatt cattatggaa ccatgttcat gatggattaa ttcctagctt
    27901 cttagaatta attgctaact cgcaattttt ctttgactta gaaaacccac aaatttatta
    27961 cgcttcaaat gctgttataa aaattgaagc tatttaataa ttctgctatt caaggcaata
    28021 tctctgattt tttcttgaat agcttttttt actacttgcc caaactcggt gagacttgag
    28081 tgtttatcga catataaaat tgcctccgaa tatgtttcgg atttttcgat tgcttcatca
    28141 gcaaaatttg aaataaactt ttcaatctgt tcattaatat tttccatttt ttctcctatt
    28201 ctggttgata ttctcgagca ttgatgcctt ttggtaggcg ccatccgcta gcagcaatac
    28261 gattaatcat gtttgatgct acttcaaatt tccacatacc tacatttttg aaaccgtatc
    28321 gctcaagcat tcgtatttgt tttggagttg ttagacctga ttgacgacgt ttatccagtc
    28381 tatctaataa tttggcagcc ttaccagcat tcccaatttc gtcagtaaat atgccaaatt
    28441 tctcaagtgt tttaagttgt ttttcagaag ctggcgccat ctcccaacca aattccggta
    28501 catagtccac taagtcttca gcgttgatag acatttcaaa ttgcaacgga tcaactaatg
    28561 cacgtttacg tttacgcatt tctgctaact gcttagcgag tgcttcttct cgctctgcaa
    28621 caacatcttt gctggctact tcttcagctt ctaacaattc aaaagctact tcagtttcat
    28681 cggtcatgtt ctcgaccatt ttttgtgcaa cttctggtgt ttttgcaatt agatgagcag
    28741 gacgacaaag ctcatggcgt tcggtatgcc ataaaaaatc taataacagt agtttttctt
    28801 tccccggcgc taatcgtgtg ccacgaccga ccatttgact ataaagagct cttacttttg
    28861 tgggtctcag tacaactacg caatcgactg tcgggcaatc ccatccttca gttaaaagca
    28921 ttgaattgca tagtacatta tatttatcat tatcaaagtc ttctaggact tcagcacggt
    28981 ctttcgattc tccattgacc tctgctgctt tgaatccttt ttggtttaga atatcacgga
    29041 atttttggga agttttaaca agtggtaaaa atacaactgt tttgcgatta gaacattgct
    29101 taaccatttc atcggcaatt tgttctaggt acgggtcaag cgccgtccca atctcacttg
    29161 ctttaaaatc tccagcttgc tgactcactg tagataggtc caaagtcaaa ggaatagtaa
    29221 ctgctgtaat ttttgataaa taaccagatt tgatagcatc taccaaagaa tactcataag
    29281 ctaaactatc gaaatattta cctagatttt gtttgtcacc acgatctggt gttgctgtaa
    29341 ctcccaaaac attgctacta tcaaagtgtt gtagaactct ttggtaaccg tctgaaatag
    29401 catgatgcgc ttcatcaaca acaattgtgt cgaagtaatc tgttggaaat ttactcaaac
    29461 gcttttctcg ctgcaacgtc tgcacagaac caacaaccac tcgataccat gagccgagcg
    29521 atgtactttc tgctttttct aaagccgtgc ccaatcctgt tgctgtcatt agcttatcac
    29581 ttgcttgttc cagcaactct gaccgatgag ccaatactaa aacacgttcg cctagtttaa
    29641 ctctatcttc aataatcttt gagaagacaa tagtcttacc acaaccagta ggaaggacaa
    29701 gaagggtttt cttcttgcct tgttcccact cttgttgcac tgctgacctt gcctcttctt
    29761 gatattctct aagtttgatg taaatcacac tcctttttgt gtaggtgtcg ttaaagcttt
    29821 tgtaatactc caaccacgat ttaatcttgt aagtaaagta atattagaaa taccaagttc
    29881 acttgaccat tgttttattg tctgagtctt acctttaaat gtgattaaat ggttatttct
    29941 ttgattattt gcttgttcgg ttgaattagc ccatctacaa ttagatggtt cataatttcc
    30001 gttaacatca atacggtcaa tagataaatt gtctcgataa ccattcgatg atgcccattg
    30061 tttgaattga atatattctt cccattcgtc acatatagtt attccacggc caccgtattt
    30121 aatataatca ttggaatttt tatctctgca gcgttgtctc attccaaccc aaatattata
    30181 aagacgctct tttctagcaa aaccatgttt agtaacaatg catccgcaac tggtactatg
    30241 accagttgct agattactac tattaacaat cgttaactta ccgcaatcac attgacattt
    30301 atattttgct tttgtatatg gatcctgagg taaacgctca atcactacaa gcctaccaaa
    30361 tcgttgacca gtgtaatctt tagcttttcc cattagaatc ctccccatcc accttgttgt
    30421 tgagatgctt gcatttgacc ttgttgatat gactgaggtg ctggttgttg ttgaggtgca
    30481 aaattagttg tttgctgagc tggttgttga tacgttggtt gtggcgcttg tggttgagta
    30541 tttgcattca atactttagt ccagtcaacg ctatcagcat aaatcatcga tttcacttca
    30601 ttataaacat tgtcgttata tgttctattc ttaacactac atacacccgt cgcaccaact
    30661 actgtgttcc aattcatttt taatggctca ccgtgttttt tctgaccaat tgccccaaag
    30721 aatgctgata acattccttc ggtagagcta tgtaaaaata gattatgctt caaactagca
    30781 acaccttcat cagtttcaat ttcaagagaa attactgctt tattacatgc tggcaatttc
    30841 cctgtttttt gtggatttgg tgtgtggcgt gcacgttcaa agtctgttac tgtgaagaca
    30901 tagtcaccag gttgtaatgt aataaattct tttgcatctt cttggattgt gtcatcccat
    30961 ccaaattcac gttcaaagtt gttattgtat tcagtcattt tatttctcct attttaattt
    31021 taaaaagtat tgttagctat tggaataaat ccaatttttg ttatattttg ctattatttc
    31081 taattcccaa aatctatgag ccttccactg taaagggctg ttctgttgct cggacttgtg
    31141 ttttaataac ttccaatgtg gctccccaat ttgctacaat catgttccag taatctggtg
    31201 gaaagttttc aataggagtc aatggaggga agtgtcctct aatatttgct actgctacta
    31261 actcatctgg tgtaacttgt tcaggcgtca tcaaatccgt caaagccttt ggtaggacag
    31321 atggatactc tgttgctgca gttggtcccg acatttgcgc ctgttgctca tgtacaggtt
    31381 cgtgtatagg ttgagtaggc accggttgct gtactggcgg tgtttctgct actggttgac
    31441 ttggtgcttg tgcgaatata tgtgcgatac cagcgtaatc aagtggcatt tcttcttgta
    31501 atccatgacg gttttttgca tcccacgctg gatgatgttg tgtatataag acacgggtac
    31561 cgccagttgc tttcttcttg ttgttatcag ctgtcatcac tacagtttta taattagcga
    31621 acagaaccat gtcagcccat tctttaacta atggtgcagt ctgtgagctt gtctttttac
    31681 ctagcttgag ttcccagcga tcataagctc ccatttcatc aggttgctca aatttacgca
    31741 tttgggcatg agcagtaagg acgacattga tacctaactc tatcaattcc tgcaacatat
    31801 ttaggaaacg tccaacttct tcttttgtgt aagtgtaacc attaccccaa ccaaaatctt
    31861 caattccttt ttttccatgt tgagcacaga catcatctac tattaagctt tctgcccagt
    31921 caatagtgtc aatgacaagt gtcttacagc atgtagggtt agcttttaca aaagcaattt
    31981 gattttttag catagtgtag cttgtaggct tgtccatgcg tgccacgtcc atgttgtcag
    32041 ttgaaccctc tgtgtctata aacaacggct ctggaaattg cgctgcaaaa cttgacttcc
    32101 caattccttc tggtccatag ataatgacac gttgtgccct tgcccgtttt cctctagtaa
    32161 tttgcatatt tacctctctt tctaaaatcc accttgccaa gtcggtgcta ctgtttcagc
    32221 aaaactacca ccctctgctg ttttaaagct atgggcttta ttttccactg agtatccatc
    32281 ttctataatg atggcacact cttcgccagt tgatacacgg gttgcaatag cttgcaaacc
    32341 ctcctgctct agccatttac caaattgttc aagtgtgatg ttgtccatct gttcgagttt
    32401 atcaatcaaa acaaagccac aatctggttt tagtttacgt acaatagcag ttgctactat
    32461 tagttgttgg ctgcctgaca tgttatccca ctcttgacca aggtaaagta actttccatc
    32521 atctactgac aagccttcaa gtggcagatc tgcattaatc aataagtctc gtttttgttg
    32581 acgaattgac tcaatatcat tagtcaatat gttatattgt tcacgctgat gtttagcatc
    32641 ttcttcagct ttatctttat ccagattagc tctgactttg cggttgactt cttcaatatt
    32701 agaaatgttt tcttctattt ctgcagttga ttcatctatt aatgacattg tgtctctttg
    32761 tgcaatttcc aagtcactag ataactcttg taattgtttc tctgctgtgg ctaattgttg
    32821 ttttaaatgt tcaacctctg actgtttaaa ttgatgattt tgttgaataa cggatagatt
    32881 ctgacgtttt ctagcgtttt ccccattttt tgctaacact tcttgttgtt gctggattaa
    32941 atcagcgata ctaattaatt cttttggggc atcattatag tatggttgct ccttagcaaa
    33001 cttttctttt tggtcagcaa taacaccaat agtatgacgt tgattataga gttctttttc
    33061 tttgagttct aattctgcca actgatcacc aactccaatg atttcaagta agacatctgc
    33121 tttttctttt ggagttccat ccataaattt tggtaagtta atagctaact cttctacaaa
    33181 gctatcaagc aattgttgac cacctttttg cccgttagga tcaataactt tcaaactaga
    33241 attcttaccc ttacgctcaa caatcaaacc atttgacatg gtaattttaa gcgtaggtgg
    33301 taccatagtt ccctctcgtg ctgcttgact aggtttaaac ttattaccac ctaataccca
    33361 agcaatacta tccagaacgc tagttttacc ctgattgtta ttgccaccga tgatggttaa
    33421 tcctttggat gatggttcaa cttttacagc tttaatgcgc tttacgtttt cgatttctaa
    33481 cttattaatt gtgattgtca ttgtcaaaat ctcctattac taatttactt tcggtaacta
    33541 ctttttcaga actaataatt tgagcgttag caagaccata gtttagtagt gcatcagtta
    33601 cttcttttag tgttaaatca cattcatttg caatattggc gatttcatca taaatattat
    33661 ttgaacatct aattcgtgag tacgtatttt ttggttgtct ttcaacttca atcattatca
    33721 tcactccaat ttttttctag tgtttgtcta tcaacgttat ctaacttagc aatacatttt
    33781 tccagtgtgc tatctaagat gtaaccttcg ttgacgataa tatccaataa atttgctttt
    33841 gctacgttcg taaaataata atctgccatt tcattagtta gtcgtctatt ttcatcacgc
    33901 aacagttcat tttctagtaa tacttctttt aacatgtacg ccctctatta ttacgttcaa
    33961 tcatattgtc gtaacgtcgt gcattagctt cccagccatg tgtttcgatt gtccattttt
    34021 gacgtgtttc tataacctga tttttcttta caaataattt tttaaataat ttcttcatta
    34081 tgttatactc ctaactgtct ttccttttta atattttcca acatttctgc cagcgtttcc
    34141 tttttaggtt tatatctatt tctagacttc caagtaacaa acaacttaaa tccctcgtaa
    34201 ttgatgaata caattctatg cgttggatta ttgataaatt gtttaaaatc tggatgattt
    34261 ctcatttctg ctgcccaaac ctttgccgtt ccgacggtta atccctccca gatttggcaa
    34321 agatgcacat aatcgccata gtcggctttt tccgactgct tagctggtct gtagactaat
    34381 tcagcttttg gcatttctta cctccaatct gtgatataat gtaagtagaa tttttgataa
    34441 ggggctgact gcaatcagtt cctttttttg catttactca atgctgtaat ctgcaatcac
    34501 tcgcagaata aactggttag cctttggtcc tctagttgag ccactaagaa tgttagtgac
    34561 ctcttgacga ttgagaccat aggctgctgc taaatcactc ttcttgatat tgttactttt
    34621 tagaaaggta acaacttttt ctcgtccaac tgcaatatct ggcatatttt ctcctttctt
    34681 ttctgatata ataaaaataa aaacttgagg taaaaatgtc tcgatactct gattataaat
    34741 acgctaatac tatccttaaa agtattgaag aacgaccaga aaaatatcta attatccatt
    34801 actcctgcga aagtttttat aatctaggta gtaaaagtcc tagaatagct tccatttcag
    34861 ttcgtcaatt caataatgcc caaaccaaca acttttccat tcatcagtat tccgagatat
    34921 tagatacccc aattactgat gatacttatg aaactatcga aaaagagctg ttaacagatt
    34981 tcttcacatt tgttgacaga aataccgaca aaaattggat tcactggaac atgagagata
    35041 ctgtctttgg ctttcatgct attgagcagc gattcaaagt tctaggcggc acaccaataa
    35101 tcattgatga tgacaaaaag atcgatttag cctatctgtt taaaaagatg tacggtggag
    35161 gatatattga caatccgcat attgaaaaat tacttgaatt gaacaatcta accccacatc
    35221 gttttctgac aggtaaacaa gaagctgacg ctttctcttc gagacaatac catgaactaa
    35281 gtatgtcaac atcaagcaag gtcaatagct tttcaacatt cctcacttta gctatcaacg
    35341 atgatttaaa aactaacacc cctaaatgga aaatgagagg aactaaccta tctggattac
    35401 tttccgcttt tcaagaaaca acctcaggaa aagtgataat cggtataatc aaccttattg
    35461 tcggcggaat agttggtgca ataattgcaa aatatattca ataaatgatt tcttttgaag
    35521 tagaataagt ttttgttgta cttgctctac ttcttttttt gcatcctctt tggaaataag
    35581 agtcggattg tcgtaccaat ctctttccac gtaaagttcg ttttctttcc cctctaagct
    35641 atcagcgacc cactgaaaat gctcattacg cttttgtaga tcaacgatgt tttttttcat
    35701 tttccctcct tgttttaaaa tctgtaaaca agaaacaact aaattttcaa ctttttagta
    35761 tataaccact tgacaactca cacctaaaaa gttagaatta aagcatatta aagaacctta
    35821 acaaatcaac taaaacccgt tcgccaaaac tgttttatag ttgtttgctt agttgttttt
    35881 ttagttatct cttgcttaca aaaaatatga tacacctatt tggttgaatt gtcaacattt
    35941 ttcacccaaa aagttgaaat attttttgtc atgccttaga aaggttgata aatcaatgtt
    36001 tccgacgttt gaaaaaatca aagaattgtg caaacagcgt ggaattacac ttattcaact
    36061 agaggaaacc ttaggatata gccgaaatac actctataag ttgaaaactc aaaaacccaa
    36121 cgctgaaaga attgctgaaa tagcagacta cttcaatgtc tctacagatt acttattagg
    36181 tagaacagat aatcccaata tagctaaaga tagccaagaa tacacttctg acgatcttag
    36241 aaaaatggca gaaaaggcta aaacatttga tggcaagcca cttactgaat cagatattga
    36301 tgcaatacaa aatattattg aaatttattt gaaaggaaga tagtctatga ctattgaaga
    36361 gattgtagac tgtcacaaag ttaatctcgc atactttgat aacgaattat ggccacgacc
    36421 tgggatttat atagacgata ttaaagttat ttttgtcaat aaatcactat cgttagatgc
    36481 tcaaaaacgg gttatcctgc atgaactcgg acattgtgaa agaaatattg gtgactataa
    36541 gacactacgt gagaaacacg agctagaagc tgacagatac atgattagtc atttaatcgc
    36601 tgaagcactt tctcaattag aagataaacg agagtttaac tacctacaat ttatgcaata
    36661 tcatcaacta acaactactg cagatgaatg catggtaatt gatgaatact ataatttact
    36721 taaggtcgtg taatatagtg aggagattat atggatataa ataaaataaa ggaatttgct
    36781 gtgtcaacat cagaaaaggc taaagacggc attattaaag caaacgaaat gagaaaaagg
    36841 gcatcacaag aaagcaaaat agtcttaccg cccacaatta atttaggttt taatacacct
    36901 actgctattc gtaaaacagt ggagggtgac tactatattg gtttttattc tgaaaaacct
    36961 gatttattta aatttgtgga ttttcagttt gatggttcac gaattattga aaaaacagta
    37021 acaaaaggaa aaacgactca aaaaggtcgt tctggtagta caattattgg aggattaata
    37081 ggtgcaacta tcaatcctac tgctgcagta cttggcggat tagctggtgc aaatcgtggt
    37141 aaaaaaggaa ctattaacag tacctctact actataaccg aggaaattcc gggtaaagct
    37201 caattaacac tcagaaattt aaaaacaaat gatataaaaa taattaaaac aaaactaact
    37261 caggctcaag ctaacaatat tcaaaatttt tttagcaaat agaaaaagcc ccactctagc
    37321 cggcaagcaa tgaagtgagg caaactgtac aataaattag gcattaaaaa gcccttttta
    37381 ttgtacccat tttatcaaga aatgaggtaa aaatcaatgt ggtcagaaaa acacaaaagt
    37441 ggaaaagtaa attttgtaga aagatataaa gatccatata ctaataaatg gaaacgtaca
    37501 tcagtactga tggaaaaaga tactcctcga attagaaaag aagctcaaag aatattagat
    37561 gctaagatag ctgatatcgt aagaaagttg caaacctctg acatgctatt tacaaatcta
    37621 attgatgagt ggtggatatt ttaccaacag gaaattaaaa gatcgtctat tgtcacttta
    37681 aaaggcaata ttcgagagat tagagcagaa tttgggatca atatacctgt agttaatatc
    37741 gaccctagat atgtccaaaa ttatctggat aatctcgatt gctctagaaa caaaaaagag
    37801 cgaaacaagt ctatgcttaa tcttatcttt gattatgctg ttagtctaga tatcatcaag
    37861 gataatcctg caagacgcgc taaactgcca aaaatcaaga aaacacttaa tgattggaaa
    37921 aagattgaag aaaaatatct tgaagaagaa gaaatcaagc ggttgttaaa agaattattt
    37981 agaagaccta gcactcaccg cctgggattt ctatcagaat ttatgagtct taacggatgc
    38041 cgtattggtg aggctattag catagagccg gataatattg attttaaaaa caaaactcta
    38101 cagttgcacg gaacttatga tcgcactaat ggttacataa atggagaaaa gacctctcca
    38161 aaaacccttg cgtcctatcg tgaaaccatt atgagcaaac gtgaaatgga aattttacaa
    38221 gagatggagt ttattaacga actcgaaaaa aatacaaata aacgatacag agacatgggg
    38281 tatatattca cgactaggaa cggtgttcct atccaaataa attcctttaa tttagcattg
    38341 aaaaaagcaa acgagcggct tgaacaacca atcaataaaa atatcactag ccacatcttc
    38401 cgtcatactt tagtcagtcg tcttgctgaa aataacgtac cactaaaagc aatcatggat
    38461 cgtgttggac atgctgatgc aaaaactact gtacaaattt atacccacat tacaaagaaa
    38521 atgaaatcca acatagctga tattatggaa aactactaac tttatgcccc aaatatgccc
    38581 caaatataaa aaaagaacct tgtcgctatc aataaacatt gatatgacaa ggtttttcta
    38641 gacgaattat ttaacagcgt ctttaagagc tttaccagct ttaaatgctg gaacttttga
    38701 tgctgcaatt tcgatttctg caccagtttg tgggttacga cctttacgag ctgcacgttc
    38761 acgaacttca aagttaccaa aaccgattaa ttgtactttt tcaccttcag caaggtaatc
    38821 tgctacagct gcaaaaactg catctactgc tgctgctgaa tcttttttag ttaattctgt
    38881 agcttctgct acttttgcaa ttaaatcttg tttgttagcc atgttaacaa atcctccaaa
    38941 taaattctaa acttatacaa cataagtttt aacagattat atgatatcta aaaaaaggct
    39001 taaggtcaag tatttaacgt cattttaggg ctttttctta aaaatatcga aactaaaatt
    39061 atcgtttact aaatcaattg cagtcgcttt caaataaata ccttctttat tttttaaatc
    39121 ttgcaaatta atattaataa ccgatttttt aggttttata tctacaaagt ttggcaattt
    39181 ataagatgat tttatatatt gtaacacatc tttttctgga agcggaagag ttcctactga
    39241 aaatgaggtc actttaagtt gaacagcgcc atttgttaaa cgataaggtt caaaatagat
    39301 gtataaaggc acttcataac ctaataattg ataagagcct tcaaatagta tagaagatga
    39361 agcagcataa atcttataat tcatcttctt agtttgatat tgttttaaat aaagtgcaat
    39421 tgtcttattc agttgcgact tattggtcgt aaaagtacct acttttactt tatcttgtac
    39481 cccagtcgaa atttttcctg tattaggctc acgtacttga attaaacgac ttgcaatcac
    39541 tgctgtgaag gaaaggttaa ttgccaacaa taagagaaat gcccatttcc aaaaatttaa
    39601 attacggcca gttttttctt gtttcattta ttttctccat aacggcgtta gacatgattt
    39661 gatagccaat attattggga tgaaaatggt ctccagtaaa gagagcatca ttagtgatac
    39721 ttgcctgact atttgatgac tctgtaatac cctctttacc atttattccc ttataaaggc
    39781 ggtcattaat tgggacaaaa taaacatttt ctgaagcatc aactacttct tttgtagctt
    39841 tattccaatt atcaataacg gtttgcattt tagttaattg tggaaagttt aggtaaaaag
    39901 gattataaat gcctaaaaca taaataggca atttaggatt atcttgtctt gcttttgcaa
    39961 gaatttcttt caaacgttcc ttatatgctt ctgctggttt ctcaaaggaa tttagtgata
    40021 aatgactgag ctctttacga ataacagcca agacatcatt accaccaaca gttagcgtca
    40081 ataaatcagc tttctctaaa tctttttcga tttgaggatc tgtcgtcata cgttttaaaa
    40141 tttgttgact agtattccca gacacaccat aattaacaga agtcacttgg taagagtatc
    40201 gattatggag tgattctgat agcagtggga caaaaccacc ttgagaggtt gtatccccca
    40261 caccttcggt cagagaatct ccaagagcaa catagttaag tgggataact ttctttgtta
    40321 ggaagtcttt ttttgttaat ttaggatttg attttggaat gatcaaggaa aaaatcccaa
    40381 aagataaaag aagacttacc agaaaaaaag aaagtccggt aagtattttt ttcttattca
    40441 taacgaacca taacagcaaa tgcatcctca ccagtatgag tttggatgat tgagccagtt
    40501 tctaatacgg atataggagc acctagaaca gctagttttt ctttaaagtt attagccata
    40561 tcagctttac cacagtagga gatgccaatt tcagcaattt ttcgtgtttg ggcactttca
    40621 acaaaattat ctaaccattt gctgaaggtc tttagaccgc gacctttaac aatcggtact
    40681 aattcatggt ttgttagctc cataattact tttatgttaa gcaatgaact taggagacct
    40741 gttacacgtc caatacgacc accttttact aaattttcaa gggtcgatac gccaataaat
    40801 aattctgact tctggcgtac ttcttccaca cgagccaaaa ttgtatctaa atcagctccc
    40861 tctttagcta atttcgcggc ttctacaacc tggaattttt gacactggtc tgtaaaagta
    40921 gaatcaataa ctgtaacatc tgcaccagca atattagctc cctggcgtga tgcttcaata
    40981 gttcctgata gtgtatgcgt taaatgaatt gcaatgatat gttccgcacc ttcattcatt
    41041 aatttttcat aaatttctgc aaaaacacct acaggtggtt gacttgtttt tggcaattcc
    41101 ttacttccac gcattaggtt caagaattcc ccttgtgctt ttaagtcatt gtctgaatac
    41161 aaggtaccat ctatcataac tgacaaggga acaactgtaa tgtcaagctc tttaataagt
    41221 tctggttcaa tggtaatgga agaatccgtt acaattttga ttttactcat atcctatcct
    41281 attctatact ttaatatttc aataccctta ttataacaaa atttaagtgt atcaacatta
    41341 atttctccta acaatgttag ctttttaaag acgttaccaa agctaactac caagtaattc
    41401 tttagcttga gtacgtgcag tatcagttac attgtttcct gccaacattt tagcgatttc
    41461 ttctacacgt tcttcatagc ttaaaagcct taccgttgaa actgttgaag agtcactgga
    41521 aattttttca ataaaatatt ggtaatccgc tattgcaata acctgagcta aatgagaaat
    41581 tgctaaaact tgtccatgac taccaatctt gtgaattttt tgagcaatag cctgggccac
    41641 gcgaccagaa actcctgtat ccacctcatc aaatacaatg gaagttttat cttccttacg
    41701 tgaaaaagca gatttaatag ctaacatcaa tcgagataac tctcctcctg aagccacttt
    41761 taccaatggc ttaaagccct ctccgggatt cgttgaaata taaaattcaa cgatttcgtt
    41821 cccttcttta ttaaattttc cttttgtaaa ttgaacttgg aaatcagctt tctccatata
    41881 aagctctgtt aattcttgtt tgatttcatt ttccaattgt ttagctagct tatgacgctc
    41941 taaacttaat tgatttgctg attcaatcaa atcgtgttct aaaatcttta attctttttc
    42001 taaagcatct gaggactctt cactacctgt caatagacta tattctttag taatgttatc
    42061 aaaatagtct aaaacatcat tcacatcacc gccatatttt cgcgttattg tgttaataac
    42121 atctagtcta ttttcaattt cttgtagtaa accagcgtca aaatctaaat catcgataac
    42181 gtcacctaaa cgtttagtaa cttcttcaat aacgtagtaa gcttctgaaa gattggtgga
    42241 aagatcttta tattctcgat caaattcttc taaagccata aggtcattca ttgcagaacg
    42301 aacattcgat aaacttgaaa actcttcgtt atctaacata agatatgcat ttgtcaaagt
    42361 atctgcaata ttcttatgat tcattaattt atcacgttgc ttgagtagcg tttggtcttc
    42421 atctgattta agggctacag actcaatttc tgctatttga aattctagca tttcaatacg
    42481 tgatttattc tcttgttcat ttttttgctt atccaataca cgtttacgaa gctgacgata
    42541 agcatcaaaa agactttgat aacgctcttt tatgacatta aattctgtat taccaaattc
    42601 atctaacatc agaatgtgca tgttaggttt cattagttcc tcttggtcat gctgaccatg
    42661 aatgtctacc aaataatgcc ccacagcttt tagtgttgac aaattcacca tttgcccatt
    42721 aattcgacta acgctgcgcc cattttgaaa gatttctcgg cgaataatta attcatctgc
    42781 taattcaatg ccattttctt ccaataattg gactaatgat tgattttttt caatagagaa
    42841 aaatccttca atttctgctt tgttagcacc atggcgaatc acttcaacac tagcacgggc
    42901 tcctaacatc atattcatag catcaataat gatagatttc cctgcaccag tctcaccagt
    42961 taaaacagtc attcctgttt caaaattaag tgaaatttct tcgataatag cgaaattctt
    43021 aatagaaatt tctaaaagca taaggtcctc tactaatctt ttttctccaa cattgcaatc
    43081 attgattgac gaatgtgatc agcatctgct tctgattttg caatcaaaag gagactatta
    43141 tcgtcagctg tcaaactaaa aatatgttct tgacaatgtg acattatgaa ggttttaatt
    43201 aattgactgt tacctggtat gacattaata ttgataaatt gctctaaacc tagtagctta
    43261 tctgatattg ataaaatact tgttttaata ggctttgcca cagcagttgt aaagataggg
    43321 tcgttttcat ttgacaaacc gtaaatatag cgaccttttg ctgatggcac ttttataatg
    43381 ccaatttcat tcatatcacg tgaaatagtt gcttgagtta gagttacccc ataagcttcc
    43441 aatctgcgta acaactcatg ttgtgtttca acagcatggt taagaacaat ttgcttaatt
    43501 aaatttaaac gttcactctt cttcattttt cttaaattcc ttatgtgctt tttctataac
    43561 atcttgtatt tggtcaagca caagattttg tggatcttga cacttttgca aatgcattaa
    43621 aaactcaata tttccatgtc caccttgaac gggcgaaaaa tcaagatgtt taaccgtata
    43681 tccataatct ttcgtgaaat tggtcactgt tgtcaaaacc ttttcatgaa ccaacttgtc
    43741 tttgacaata ccatttttac caatttgctc acgacctgct tcaaattgtg gtttaattaa
    43801 tgccactact tgtccaccat ccactaaaat ttcttttaga gctggtaaaa tcaaattaag
    43861 agagataaat gagacatcta tcgatgcaaa ttcaggcagt ccctccttga aatcttcttt
    43921 ttgggcatac ctaaaattat attgttccat agaacgaaca cgatgatcct gacgtaactt
    43981 ccaaactaat tgatttgttc ctacatctac tgcgtaaact aaacgcgctc ctgattgtag
    44041 cataacatca gtaaaaccac ccgtagaggc gccaatatct atagttagct tatctgcaac
    44101 tgaaatttca aaaacttgta aagctttttc taatttcaat ccacctctac taacatattt
    44161 tagtttttca ccttttagtt ttaattcagt atcgtctgca accttttcgc ctggtttatc
    44221 ataacgttct ccattgataa cgttaatcac cagtcctgcc ataacaccac gtttcgcttg
    44281 ctctcgtgta tcaaaaagtc cctgtttata ggcaagaaca tctaccctct ctttagccat
    44341 tcaaccgtaa tccctctatt atatctatta tttttctagc attaaagtta gcttttttct
    44401 ctagattttg aaaaattgcc tgagcttttt tcaaagtatc gtcaagtatt tcctgtgact
    44461 tatccaaacc caataaattt ggataagttg tcttttctgc tacaatgtct ttatttggtg
    44521 tcttacccaa ttcttcaaaa ctagcagtca catctaaaat atcatcacgt acttgaaaag
    44581 catgaccgat taaaaggcca gcttgatgca gttttccttt tacttcttcc gacttctcag
    44641 ctaaaatacc tgctgcaaca aagggataag ctaacaggcg tcccgtttta ttaatatgaa
    44701 tcaagcttaa atcgtcaata gataaaactt tgttttctcc cttcatatct aacatttgtc
    44761 caccaaccat gccaaaacta cccgcagaca tagacaataa ctctattaac ctaacagtaa
    44821 catcagcttt aaaaccagct tttacaacta aatcaaatgg gtctaaaaaa agggaatctc
    44881 cagctaaaac cgctgtagct tcatcaaact ttttatggtt tgttaagcgg ccacgacgaa
    44941 aatcatcatt atccatcgcc ggtaaatcat catgaattaa agagccagtg tgaatcatct
    45001 ccaaagcagc agccacatca taatgaccat ctattagttc aactccaaat ccctctaaaa
    45061 tttctaaaag gatgcgtgga cgaatacgtt tcccacctcc atcaactgag tagaggatag
    45121 ccttaattaa atcaggagaa acgacactat gtgtctgttt gtaatgacga tgaatagctt
    45181 catcaatttt ttcaatagta accatcaagt atccatttct acttccgttc cgtcagcttg
    45241 catcactttt acaagcgtct cttcagcttc ttttaaagtt ttttgtaact cttttgaaat
    45301 aagcattcct ttttgaaatt cagcaatggc atcttctaag gcaacatcac ctgtttctaa
    45361 acgtgagaca attgtctcta attcctgcaa attttcttca aatgttttct tgtctgacat
    45421 ccttcacctc aacttctaaa agaccatctt gcatcttaac ttgcaaatga tctccttcat
    45481 ttattccatt agtagtagat actaaagtat ggtttttttc gataatagcg taaccacgcg
    45541 ctacaattct agaactatcc aatgatataa gcgcatcttg agccttttca aaacgtgcta
    45601 gcttgctgtc atactgacta gtcattgtac ttgataatag tcgtctactt tgcacaacac
    45661 gctcttggta agtatgaagc cgctgtttta aatctatgcc ttgcagtttt tgatgaagca
    45721 aaccttgctt ttgtcttact gtttgtagtt tatcacgcat agaatatgtt aactgctgat
    45781 ttaggttatc taatttttga aggaaaccat catatagacg ctctggttgc ctaaaaataa
    45841 ctgattgctt tgatttttgc aacctctcct cttttgtcct gataagacga aggctagact
    45901 gatacattct attttcacgt tcagtaatcc aagacaagat atcaatttta gtaaccgttg
    45961 tagcaagctc agctgctgca gttggagttg ctgctctgcg atcggctaca aaatctgcta
    46021 aagttgtatc agtttcatgt ccgacactag aaataacggg aagacgagat tcaaaaatag
    46081 cttctacaac acattcttca ttaaatgccc ataagtcctc aatagaacct cccccacggc
    46141 caacaattaa tagatccaaa tcctttttct cgttagctaa agcaatagtt tgtgctatct
    46201 cctgagctgc gccctctcct tgaacttttg tggggaaaag taatatttcc actcctggga
    46261 atcgacgaga tactgttgta ataatatccc gaataacagc tccacttgga ctagtcacaa
    46321 ctcctatttt tctaacaaat tgaggaagta attgcttatg acgatcatca aagtaacctg
    46381 cttgagagag tttcttcttt agttgttcaa attggatagc aagagctcct ataccatccg
    46441 gttctgcttt ttcaacaata atagagtagg agccgcttgg ttcataaagt tgaacacggc
    46501 caactacatt aaccttcatc ccttcctcta attcaaaacc cagttttttg aattgacctg
    46561 accacattgt cgcctgaatg actgatttat catcttttaa agagaagtat tgatggtttg
    46621 ggcgacgacg aaaattagac acttgcccag ttaaataaac cctctctaaa taagggtctt
    46681 tgtcaaattt taattttaaa tacttagtca gtgtcgacac ggataaatag tctgacatag
    46741 cacctcctca tgagagtttt attaacttca ttataccaaa aaaacttaaa aaatacctat
    46801 ttgctctaca aatagctcga taaagcaaaa gaagtgtcat gctaacacct ctctacttat
    46861 tttttattta gatagtctag ccatttcttt tggaatctca tcactaatcg ttgtcggtag
    46921 aacaacgtag gcttctttag ataattcttg agcaattgcc gaatgtagat aaactcccac
    46981 tgatacctta tctagaggag aggcttcttt aaattgcgct aacatacctg caatcatacc
    47041 acacaaagta tcccccatcc ctccagtcgc ctggtaagga cctcctacta taatttcttt
    47101 ttcgtctaaa tcttgaaaaa tacgcgtatg cgaactctta gcgactaaga tcgtcccttt
    47161 gggaaaagat ttaagagcgg tttgggtatt ttcttttgtc tgttgcgata cagcaatacc
    47221 tgacaatcgc tcccattctt tttgatgagg tgtcaacact aaattcttag cctttgtttg
    47281 cggaaacgca ccttctgata acagagtcaa ggctgatccg tcaatgatga cactttgatg
    47341 tgattgaatc ttctccatgg tccggttcaa aatttttcga gatgattctg atactcctaa
    47401 tccaggacca attacaataa catcactttg aacaattgat ttttccaacc atttgtaatc
    47461 atcactatca aacgccatta cctctggtag ttgactatgc aaagagggga tattgcaact
    47521 ttgggttgct acagtaacta atcctgcacc agttttgaca caggccaaag ctgccattat
    47581 aatagcacct ccatagggat aaaaacctcc tatcagaaga acactaccat aatcaccttt
    47641 atggcttgat ttagggcgtg gcttaattaa cgccctcgct tcttgttcac caacaatcat
    47701 agactcacac ttctaagagc tgcttgataa gtttgttcca aaagcattgt aatcgtcata
    47761 ggtcctacgc ccccaggaac aggtgttatc atactagcaa cttctgccac ttgttcaaat
    47821 acaacgtctc caatcaattt accattttca tcgcgattca taccaacatc aatcacaaca
    47881 gcaccttctt taacgaagtc ttttgtaaca aagtgcccct gaccaatcgc aacaataagg
    47941 atatcagcct cctttgttac ctcagaaagg tttcgagttc ttgaatgtgt caaagtaact
    48001 gttgcgtttt tatcaaggag aagttgtgcc attggcttac caacaatatt ggaccttccg
    48061 atgataacag catgttttcc ctctaaatcc acatggtact ctctaaacat ctccataata
    48121 ccagctggcg tacaaggtac catcatcggt cgtcctgacc aaagatgacc cgtattcatt
    48181 gggtggaaac catcaacatc tttcttagga tctattgcta aaataatttt tttatcgtta
    48241 atatgttgtg gtaacggaag ttgaactaaa ataccatgga tacttttatc ttcattgtat
    48301 tgatggataa tatcaatcag ttcttcttga gaaatggatt cagataatcg taaagtctca
    48361 cttttaaaac cagcttctaa tgcagagcgt tctttatttc gaacgtacac ttggctagct
    48421 ggattatccc caacaaggat tacagctaaa ccaggtatga taccatgctg ttcctttaac
    48481 ctttcaactt ttcgccctaa ctcagcctgc attttttgtg ataaggcttt accatctatt
    48541 aattcagtca ttgatactcc ttcttcatcc gttttttcta tattatatca aaaaatagcg
    48601 attatgctat tttatatcgc tattcatttt gtatcaattt taatcaatag aaaaggcaat
    48661 gaatccctca tcgccttttt acatatgact tatttagtac tattcatttt agcacgacaa
    48721 gccgactcaa ttgcattaag tttcgataaa ctatctgctt ctgtacaacc aatcgtataa
    48781 aggtaacatt ttatcttagg ttctgtccct gacggcctta aagcatacca tgaaccctca
    48841 ttaaaataat attttaaaca attttgcttt ggaaaatcct tataaccatc cttgaaatca
    48901 atagaattct ccaatgtcat ctcacctact tgtaatattg ggttctgtct aaaatcctcc
    48961 ataatacgac taatacgttc ttgcccctca gcaccctcta actcaagaga aaattggcgc
    49021 tcgttgtaat agccaaattc atcgtaaatg gtttgcaaaa cgtctaaaag tgtttgccct
    49081 cgttctttat aataggcagt catttctact accatcattg aagcactcac agcatcttta
    49141 tcacgtacaa aagtgccata gcaaaaacca atactttctt catagccaaa gagataagtt
    49201 ttatcctttg agatatcata ttcattagct tttccacaaa tatttttaaa tcctgttaaa
    49261 gtttcaacag tttcaatatt atatttatct gcaataactt ttgatagatc accagttaca
    49321 atggatttta ccaatacagg atgatgtggc aaattcccta aggcacatcg ttgtgaaaaa
    49381 atataatagg aaagaagtgc ccctatctta ttaccattta agaaaatata ttctccctta
    49441 ctatctttga cttccaatgc tactcggtca caatctggat ctgtggcaag taagacatct
    49501 gcatcaactg actttcctag agattctgaa taggcaaatg ctttaggaac ttcagggttt
    49561 ggatagccaa ctgttgtaaa atcaggatcg ggcatttcct gctcaggtac tacataaata
    49621 ttttcaaaac cacggcgtct taaaacttcg cgcacaggta aatttcctac gccatttaaa
    49681 ggagtataaa ctactcggac tgacttatca atgttagtat cattaatagt taaaccaagc
    49741 acttcttttt tatatgcttc ttcaatactc tcatcaatat aacttgccaa accacttgcc
    49801 agagcctctt caaagggtat ttgcttaatt tgctgataat cggttatagc atccatatga
    49861 ttggcaattt ggtcagcaat atcatctaaa atctgagatc cttctttcca atatgcctta
    49921 taaccattat aagcttgagg attatgacta gcagtaacca tcacacccga aacacatcct
    49981 agagcacgaa tagcatatga gcacattggt gttgggcgaa tccctttata aatataagac
    50041 ttaataccat ttgctgccat aatggaacaa gttaattctg caaattcctt agattgataa
    50101 cggacatcat aactaactgc aattccacgt gcaatagctt cagggccatg atcaataatc
    50161 gtattagcta atgcttgtgc tgctttcccc accatataag tattcatacg attggttcct
    50221 gctccaagtt tacctctcaa tcccgccgtt ccaaattcta atgttttgta aaaacgatcc
    50281 tgaatttcag attcatctcc tttaatagct tctaaatctg acttaatatc tttaccgagt
    50341 gagtcgtttt ctaaccactc ttgataaatc tctttataat tcatatgaga catcctattc
    50401 taagaccaaa atggtaaaaa tcagctccat gataagagat tttagttctt caggagctaa
    50461 ctgaatagta tcaaattaac cttataaagt caacttattt tataaattaa ctacacttaa
    50521 tatatttaaa gcatctaaat ttttttcatc accctatcat tcttaatcta cattatatat
    50581 agacttacca acttccaaac aatacagtaa caatttactc ataacaatgg aataataaaa
    50641 gtctaaaaca agttgtttta gacaatgaaa ttataatact ttacttaaga agtctcttgt
    50701 gcggatttct tttgtctgct caaatacttt cttaggggtc ccttgctcaa caataatccc
    50761 tgcatccata aaaatgacac gatccgctac ttcacgtgca aaacccattt catgagtgac
    50821 aataaccatc gtcataccag atttagctaa atcttgcata acagtcaaga cttcacctac
    50881 catttcagga tcaagagctg aagtaggttc atcaaaaaga aggacatcag gattcattgc
    50941 aagacctctt gcaatagcaa tccgttgttg ttgtcctcca gataagcttg ctggataagc
    51001 attagccttc tctttgagtc caactttttc aagtagctcg tatgcttttg tctgagcatc
    51061 aagcttagaa agtccctttg tcttaatagg tgataaagta atattttcta gtacagtcat
    51121 attgggaaat agattgaact gttgaaaaac catgcccatt ttttcgcgca ttttaaaaat
    51181 atcattcttt ttgtctgtta tatcaatccc ttcaaaagtc actgttccct ttgttggtac
    51241 ttccaagaga ttcattgttc ttaaaaatgt tgacttacca gagccagaag ggccaataat
    51301 aaccaccact tctccttgat gaatatccaa gtcaatgcct tttaaaacct catttttccc
    51361 aaaactttta tgaagtcctt gtattgaaat aattggctca accatctatt tttacccctt
    51421 tcccaagata tttctccatt tgttttaaca aagctgagag aatcgttgtc aacattaaat
    51481 agtaaaatgc tgcaaataat aacggtgcaa ctggtgagta agtagccgtt acaactgatt
    51541 gtgctccgtt ccataattcc atgacaccaa tagtttgaag gagagcacta tctttaataa
    51601 ttgtaataaa ttcattccct agagcaggta aaatattttt aaaagcttgg ggtaagataa
    51661 catagcgaag tgtattttta ggtcgaatcc ccaacgagta agctgcttct atttgtccag
    51721 atggtacagc ctcaatccct gcacgtacaa tttccgaaat ataggcacca ctatttaagg
    51781 aaatgataat gataccaggt aaaagtcgtg taaaatctaa atctaaaaca ccaaagctaa
    51841 ttgttggtaa attgttaaaa tgcatccatg cgaaagcaat cataatttga actaccatcg
    51901 gtgtcccacg aaatacccat acatagaaat tagctaatat tgtgagaaaa tgtaaattag
    51961 tacgctttac taaagcaatt aacacgccta taatagttcc aaaaaaaaca acacatgttg
    52021 aaatcataat ggttaccatc acacca
//
LOCUS       DATRSG010000008        46361 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRSG010000008 DATRSG010000000
VERSION     DATRSG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46361
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.008"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            153..2222
                     /locus_tag="VBD89_000435"
     CDS             153..2222
                     /locus_tag="VBD89_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO7134991.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFVKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELIRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSRF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLKNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELESYSLRDSQSSIPY
                     QLLDLKK"
     gene            complement(2366..3433)
                     /locus_tag="VBD89_000436"
     CDS             complement(2366..3433)
                     /locus_tag="VBD89_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7134992.1"
                     /translation="MAYYRKRDNGWEYRISYKDESGKFRQKSKSGFKTKKLAQAAARD
                     IEKKLSQNILTDGEVTLYDFVKTWSEVYKRPYVKDKTWETYTKNFRHIKTYFKDIKVK
                     DITPLYYQKRLNEFGEKYAQETLEKFHYQIKGAMKVAVREQVIHFNFADDAKVKSQIE
                     SRAEENDFLEESEYKALLSLTRENIQYVSYFTLYLLSVTGLRFSEVMGLTWSDIDFKN
                     DILDINKSFDYSNTQDFADLKNESSKRKVPIDSNTIDILREYKKNHWQANIKNRVCFG
                     VSNSACNKLIKKIVGRKVRNHSLRHTYASFLILNGVDIVTISKLLGHESPDITLKVYT
                     HQMEALAERNFEKIKNIFLVA"
     gene            complement(3557..3961)
                     /locus_tag="VBD89_000437"
     CDS             complement(3557..3961)
                     /locus_tag="VBD89_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEO7134993.1"
                     /translation="MALFGGKDKEIEVELFTSEPNERVFEFKKSKTIVRIDDYFIRIA
                     RKSNMSNMLLHGLDGEKSILLSEITAYQLKEPSSTVGYLQLVYPGSSDTKGGVFDAVK
                     DENTVTFSKDEKIAILELKNAIENALKNKVKK"
     gene            complement(3983..4729)
                     /locus_tag="VBD89_000438"
     CDS             complement(3983..4729)
                     /locus_tag="VBD89_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO7134994.1"
                     /translation="MKYTPLSREQYFDYHNKAYQIISSIDKMKENVSYQDVIKHFEQN
                     YPILFNFLDYDEMKERYPELPDYHPTDKDIKYRGLVSNRTVTYTDRVLCESCAGLTVP
                     DLDLGRYIIYINQHTNTKGRVIFTILHELSHIHCHLNQTENQPIYMSLMSKNASEKYP
                     KELIPIEKEADTVASILYLTDERLKKALTTRESFEDIQRETDISKPALHNRLMNYLIY
                     NLQYAESYALKLVLNYRQGGGQIFNILRLQ"
     gene            complement(4738..5118)
                     /locus_tag="VBD89_000439"
     CDS             complement(4738..5118)
                     /locus_tag="VBD89_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134995.1"
                     /translation="MFFTFEKIKELADKQGISLNQLEEQLDFPRNTIYNMKKSTPNVE
                     RVSKIADYFNVSTDYLLGRTENPKIADGQDNTAVDDLTQQAIVLFRKETEGLSASEKE
                     RFNSALEGLMKTARQLIQDDSNWK"
     gene            5406..5570
                     /locus_tag="VBD89_000440"
     CDS             5406..5570
                     /locus_tag="VBD89_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134996.1"
                     /translation="MGIGSMTVTVKVTNMDKFIELSKEFNKKARELEELAHELKSFDF
                     EGEVVSTDSN"
     gene            complement(5553..6023)
                     /locus_tag="VBD89_000441"
     CDS             complement(5553..6023)
                     /locus_tag="VBD89_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134997.1"
                     /translation="MNLNQIRIIEACHKFLIGITNFEEELQDDVLVYRYKGNLVSFET
                     YQEYEQRSFVDYNLKYGYLDDTRTYIDDRADLIAAFPSEEHLRALQRVNDAEQARVQI
                     FKLLSQVNLDSLSEKNSHIKKDNFGYDFFNFATKEEYPVYLFSDDESFELVAIS"
     gene            6084..6296
                     /locus_tag="VBD89_000442"
     CDS             6084..6296
                     /locus_tag="VBD89_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134998.1"
                     /translation="MSQQQKKWIQLVKDKLNEEQMTQTHLARACGVAKATISELLKYG
                     KGSVRLKNKVSDILHIDESWTDLEED"
     gene            6296..6637
                     /locus_tag="VBD89_000443"
     CDS             6296..6637
                     /locus_tag="VBD89_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134999.1"
                     /translation="MPITREMTITEINVLNAIKNSATYDLPIQARELRQQLGLSKRSL
                     EAVIENLRVIYKQPIVAKKKQPSGYYLPRNEEERNDGLAPYKRQILTEQNNLAAIMSV
                     NLEEYWATEKA"
     gene            6684..7214
                     /locus_tag="VBD89_000444"
     CDS             6684..7214
                     /locus_tag="VBD89_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO7135000.1"
                     /translation="MNEVWKDIPIVKGYYQVSNLGRVRSIGRTVNAKQRTRKTKGRIL
                     KQSLSSGYAIVTLSVNGLRKSIRVHRLVAEAFIPNPINKRTINHIDENKLNNRVDNLE
                     WATDKENANHGNRTTKSSLGRCKPVEQFTLEGEFINTFDSIKSASMKTGISSQRITAT
                     AMGHQKQTHGYKWRYA"
     gene            7214..7498
                     /locus_tag="VBD89_000445"
     CDS             7214..7498
                     /locus_tag="VBD89_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7135001.1"
                     /translation="MNDLMIQMLDQFEAGLMDRTMKVMHVVTDEKYRFPLELNKSQCA
                     LMLLGTKDTGTFDERFNCHKDFPRIPNAREKYPRDEVIEWYHKNWQRTVI"
     gene            7522..7740
                     /locus_tag="VBD89_000446"
     CDS             7522..7740
                     /locus_tag="VBD89_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135002.1"
                     /translation="MNNLQIIAVGTLISVTLIESLISNIKLRKALKNKQTAGTVIAQY
                     IPQRGFIDYKTGRRVDIDPETKKEVFVD"
     gene            7749..9080
                     /gene="dnaB"
                     /locus_tag="VBD89_000447"
     CDS             7749..9080
                     /gene="dnaB"
                     /locus_tag="VBD89_000447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO7135003.1"
                     /translation="MDELKVLPHDIQAEQSVLGSIFIKPEKMIEVAEYLKPNDFYRPA
                     HKILFKAMVSLADRGEAIDIVTIKSTLESTDELGMVGGISYIAEIVNAVPTSSHAEHY
                     AKIVAKKAQLRSIIDNLSDSIGNAYDEDMDIDEIIAKAERSLIEVSQASNKSSFRPIH
                     DVLLENHSKIEERSNNTSQITGIETGFYDFDKLITGLHEDQLIVLAARPAMGKTALAL
                     NIAQNVATKSNKAVAVFSLEMGAESLVERMLSAEGTIINHHIRTGNLTVNEWQRLIYA
                     QGQLAEAPIFIDDTAGVKITDIRARARRLSQETDGLGLIVIDYLQLIQGSRSDNRQQE
                     VSEISRQLKIIAKELKVPVIALSQLSRGVEQRNDKRPIMSDLRESGSIEQDADIVAFL
                     YRDAYYQDKKEGQPENDITELIIRKNRHGNLGTVKLYFHKEYTKFSSVEEE"
     gene            9082..9684
                     /locus_tag="VBD89_000448"
     CDS             9082..9684
                     /locus_tag="VBD89_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135004.1"
                     /translation="MKKFDHYFSNHIAVKNESDTVTIVDYYNPEKGYEYNLRYIFDRK
                     NSSLAITGDFGELVARNFYNMGEWDTFYNHYTGHLGYFLEKLLCSSRPVHYYDTDKAK
                     REILNEFFDGADYDDLDFDDEFLLDQLFDNFDEDKGFTYILPEAFERFSEDMEEYEVY
                     EFLSRAGRYVNDVFELYLDAYKRAYEYLTKEVDDGTTQNV"
     gene            9662..10408
                     /locus_tag="VBD89_000449"
     CDS             9662..10408
                     /locus_tag="VBD89_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HEO7135005.1"
                     /translation="MAQRRMFSRKITETDRFLEMPLSSQALYFHLNMGADDEGFIDKA
                     KTIQRTIGASDDDMKLLIAKGFLIPFDSGVVVIRHWRIHNYIQSDRFQSTLYQSEKAQ
                     LEYDKSKTSSLKPIGNCIQNVSKMETQVRLSKGSLDKDSLTTYPTVSDNEEEDIPYKE
                     IISYLNEKANRNYRPNIQKNKTLIKARWSEGFRLDDFKHVIDTTVKDWSGTKYEKYLR
                     PETLFGSKFEGYLNQAPRIKTETIDGRLGF"
     gene            10409..11230
                     /locus_tag="VBD89_000450"
     CDS             10409..11230
                     /locus_tag="VBD89_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7135006.1"
                     /translation="MNPFKNFETRQVLEETCEVHGCQLWLTKVPIKGRLEELKQCPEC
                     TKAAINIFENKLNSQSKINSKLADTYAVFERDSLVSDKLRGKSLENYEIKDDIDQQAI
                     NYAKRMEQFYRQDRTGNAIVTGPSGVGKSHLTYGLAKFMNEQFKAYESPKSVLFISLV
                     SLFTKIRESFKADNGYRQAEMIELLTSVDYLFLDDLGKESRKGDSQNNEWTHQILYEI
                     LDNRSNTIINTNLSSKEIKALYADNYGNGALSSRILEGVTGNSFAYPKDMEDRRY"
     gene            11230..11478
                     /locus_tag="VBD89_000451"
     CDS             11230..11478
                     /locus_tag="VBD89_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135007.1"
                     /translation="MSYKATFMLYGYKAVPIGLFDTKELAAQAIVDHIIENSGIPRPR
                     VVATMKIRSKEIRIDYGSKTCYYLITLINNKTEEKQHD"
     gene            11471..11965
                     /locus_tag="VBD89_000452"
     CDS             11471..11965
                     /locus_tag="VBD89_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO7135008.1"
                     /translation="MTKTMTLEEKVEQWFIDRNLHEANPVKQFQKMMEETGELYSGIA
                     KDKSEIIYDSLGDMQVVLIGIEQQIKNGAQIEASPQDMELLLLASSLGEMAQKLYKHI
                     FHNETKTPLIRPELSLLHSTIHAIAIHNLTTADDCLSIAYEEIKDRKGKLINGIWVKE
                     EDLK"
     gene            11962..12291
                     /locus_tag="VBD89_000453"
     CDS             11962..12291
                     /locus_tag="VBD89_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEO7135009.1"
                     /translation="MKNMNWKAFFNNLKAFASVIIITVFVLGFNYLFYEIGYQKGHQE
                     ADRVIIYVADNAGAEMFGKITDKEIIEGRHTVTAGAYGKFLVTEEQYNEITVGDDIPD
                     YLKGRGN"
     gene            12291..12710
                     /locus_tag="VBD89_000454"
     CDS             12291..12710
                     /locus_tag="VBD89_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO7135010.1"
                     /translation="MTKCNFEAGHKPIDTIGQLAKEVLDGKYGDGEERRRNLGCLYDL
                     VQTRVNYMVHERDSKFTGIKIYDNVNKPSHYQGKNGMEAIDVVRNFIGQLTGVSAFYW
                     GNSMKYLLRFQGKNGLEDLKKARKNLDWLIEEMEKEK"
     gene            12707..12913
                     /locus_tag="VBD89_000455"
     CDS             12707..12913
                     /locus_tag="VBD89_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135011.1"
                     /translation="MKKSIGILEFKEFDAFVGGKVMPVYLHSANPNVPTTNKNEAMRV
                     TKKRYRKEFSDKYIFRKVGEVDDQ"
     gene            12903..13319
                     /gene="ssb"
                     /locus_tag="VBD89_000456"
     CDS             12903..13319
                     /gene="ssb"
                     /locus_tag="VBD89_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7135012.1"
                     /translation="MINNVVLIGRLTRDVELRYTPSNIANATFNLAVNRNFKNAAGDR
                     EADFINCVMWRQQAENLANWTKKGMLIGITGRIQTRSYENQQGQRIYVTEVVADSFQI
                     LEKRDNSTNQASMDDQLPPSFGNSQPMDISDDDLPF"
     gene            13900..15219
                     /gene="ltrA"
                     /locus_tag="VBD89_000457"
     CDS             13900..15219
                     /gene="ltrA"
                     /locus_tag="VBD89_000457"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO7135013.1"
                     /translation="MIAQKAINIFEKVQVFQRKIYLSTKADNKRKFGVLYDKVYRKDI
                     LKVAWFYVKRNKGSAGIDDFTIEEIEAYGVQKFLDEIEDQLRNKKYQPKAVKRVYIPK
                     ANGKKRPLGIPTVRDRVVQTAVKIVIEPIFEADFQEFSYGFRPKRSANQAIREIYKYL
                     NYGCEWVIDADLKGYFDTIPHDKLLLLVKERVTDKSIIKLLSLWLEAGIMEDNQVRSN
                     ILGTPQGGVISPLLANIYLNALDRYWKNNRLEGRGHDAHLIRYADDFVILCSNNPKKY
                     YQYAKQRIDKLGLTLNEEKTRIVHATEGFDFLGYTLRKSKSHKSGKYKTYYYPSRKSM
                     KSIKGKVKDVIQTGQHLNLPDVMERLNPMLRGWANYFKAGNSKQHFKSIDNYVIYNLT
                     IMLRKKHKKSGKGWREHPPSWYYNYFGLVCLRKLSTNINDDSQRYGR"
     gene            15352..15612
                     /locus_tag="VBD89_000458"
     CDS             15352..15612
                     /locus_tag="VBD89_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135014.1"
                     /translation="MNEYKKPTYIVIQEAMAERIRFLEDELYNRAYKDIERLEVENDR
                     LKVRCLDLQLENADYVWDDMCRSAIARAKSENRRPHRRWRGR"
     gene            15609..15956
                     /locus_tag="VBD89_000459"
     CDS             15609..15956
                     /locus_tag="VBD89_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135015.1"
                     /translation="MKLRLEELRNKLGLSRRDVHEATGISMNTLLHYEKGGTPSIGQV
                     ENIAKAYNINPAWLLGWIDEGQQPQPLETVVEKIIYVEKEGCRLPPYWNNDNNGRIIK
                     WKESRRAFQRRTI"
     gene            15953..16417
                     /locus_tag="VBD89_000460"
     CDS             15953..16417
                     /locus_tag="VBD89_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135016.1"
                     /translation="MTKKTASKTRRDFLEFELEAKYLKIDKLIGQRRHELERIYAVKN
                     LTMPGIDDSGASRSGTSTNTSENLAVAYASDPMILKLEEFQNAISELLEVLEPDDKKI
                     FHLRWGEHTGYDWIQVWHIMENGETGYLYRHSKQIYRRREVILDTLAKLLFM"
     gene            16527..17069
                     /locus_tag="VBD89_000461"
     CDS             16527..17069
                     /locus_tag="VBD89_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7135017.1"
                     /translation="MNIVEPLRDKDDIQAMKDYLSSWNEKYYMLFLLGINTGFRVGDI
                     LKLKVKDVQGWHIKVREQKTGKYKSIKMTRPLKNELREFVKDKELHEYLFQSRVGKNK
                     ALSYKTVYWFLKRAAEDLGIDNVGTHTMRKTFGYHYYKKYKNVADLMSLFNHSSPAVT
                     LIYICVRQDELDTKMSNFSL"
     gene            17359..17646
                     /locus_tag="VBD89_000462"
     CDS             17359..17646
                     /locus_tag="VBD89_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135018.1"
                     /translation="MKDENAFDGIRLEAGSTLEIYLTKNDLEHIANGYEVTLDIKPNE
                     TVNKIVIKPSFVNNILNPLINYDKRIVSEADLKFRDISREVARDSFALGSM"
     gene            17646..18005
                     /locus_tag="VBD89_000463"
     CDS             17646..18005
                     /locus_tag="VBD89_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO7135019.1"
                     /translation="MGYRNPKHSDWFRAWQIKFYNSKPWRTLRNKIRKAKRMRCDMCG
                     RLIHGKSIVDHIIEIDETNYQDESITLNEENCQLLCLECHNTKTFQSKINLNLDNRNI
                     NLFLIFLFLNFCGLPPF"
     gene            18093..18578
                     /locus_tag="VBD89_000464"
     CDS             18093..18578
                     /locus_tag="VBD89_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135020.1"
                     /translation="MILKINEVLEKLGISRATLTRYRKKLGIFEETRSNITKSQFKEL
                     EKLANQRQKYTRQERVELSRKTFKLIPKEKMLEISDNDSVVLKNLKTQYNHNQKVIEN
                     FQLEINKVINDGELPDKYLLDGMEKYQKLNMQIMSTIEKQSPQGDSLKEMIQEKLARY
                     G"
     gene            18571..20283
                     /locus_tag="VBD89_000465"
     CDS             18571..20283
                     /locus_tag="VBD89_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO7135021.1"
                     /translation="MVEMRYFDKYAQLIYTGKIRICKLTMKSIRRVERYKEQYLFKQE
                     EADKRIEFIEEECSNTKGLAGKLRLALPQKVWLETTWGFYHTVEVTKTNPDTLEEYTD
                     YEERRLIHEVPIIVPRGTGKTTLGSAIAEVGQIIDGEWGADIQLLAYSREQAGYLFNA
                     SRAMLSNEESLLHYMREADILRSTKQGILYETTNSLMSIKTSDYESLDGTNAHYNIFD
                     EVHTYDDDFIKVVNDGSSRKRKNWITWYISTNGTKRDKLFDKYYNIWVDILDDKIIND
                     SVMPWIYQLDDVSEIHDPDMWQKAMPLLGITTEKETIARDIEMSKNDPAQQAELMAKT
                     FNLPVNNYLAYFSNEECKGWSDKFDESLFVGDDERNARCVIGIDLSDVNDICSISFMV
                     VRGEERHYLNKKFMPRHTIETLPKELRDKYTEWELSGMLHVHELDYNDQAYIFEELRQ
                     FMSDNRILPVAVGYDRYNARELIRLFNDYYGDICHDIPQTVKSLSNPLKVYKEKAKMG
                     KIIFDDPVATWNHANVRVKIDANNNIFPNKEKAKEKIDVFASQLDAFICYENFKEDLS
                     YYFD"
     gene            20292..21434
                     /locus_tag="VBD89_000466"
     CDS             20292..21434
                     /locus_tag="VBD89_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO7135022.1"
                     /translation="MNNYLKNLKEVFARIFRPNNRKSTRTYLQRSISYWRRNSIYLDN
                     IYNKISTDTAQLRFKHVKITRNPGGVDSMVWYEHSDLAEVLTVSPNPLEVPVVFWSNV
                     TRAMLRDGVAVVVPRWKNGRLVEIWLAKKTVTWTAESVELMLDDVAVELPLTDVWVFE
                     NPKLNVTAQLNQITELIDINLNALTEKLSDGNSSLRGFLKLPTKAADEHLKQQARDRV
                     DSMLDLAKNGGIAYLEQGEEFQELSKDYSTASKEELEFLKSQLYNAHGINEKLFTCDY
                     TEEQYRAYYSSVMKLYQRVYSEEINRKYFTKTARTQGNKLLVFFDMADMISFKDLVEG
                     GFKSKYAGLMNSNEFRETYLGLPGYEGGEVFETNLNAVRIGAEESE"
     gene            21484..22026
                     /locus_tag="VBD89_000467"
     CDS             21484..22026
                     /locus_tag="VBD89_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEO7135023.1"
                     /translation="MEKLKTFVVKSVEDESADFHFEAYASTYDNTDREGDVMAKGCFD
                     NTLKSKAVVPMCLNHDRNCVIGKHELSVDEKGLRTRSTFNLSDPEAKKTYDLMKMGAL
                     DSLSIGFFIKDYEPIDAKQPYGGWIFKEVEIFEISVVTVPANPQATVDNIKEFDMSAV
                     DKRIAQVNMRQEIMSKLAKI"
     gene            22037..23299
                     /locus_tag="VBD89_000468"
     CDS             22037..23299
                     /locus_tag="VBD89_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO7135024.1"
                     /translation="MKKSLIELLEARQKATDELAEVKLKKATIEAKMKSSTIEDDDLE
                     QLKTDAESLVSQATAIKETIAGLDSDIEETEEELSKAAKIIKEKQKGNTPMDYLKTKA
                     AALDFVRILMDNEGSANSARKAWEANLVEKGVTNLTKILPEPVLIAIQDAFTNYNGIL
                     NHVSKDPRYAVRVALQTQVSQAKGHKAGKTKKDEDFTFLDFTINSATVYIKYAFEYSD
                     LKKDTTGAYFNYVMKELAQGFIRTIERAVVIGDGKSNSAEDKITEIKSIAEETEANLF
                     ETQEINVAGVFDNAVLETLVAGIDKMVPNTTPILVTSKAIARKLKLVKDAEGRYIDPQ
                     PFAPIATNGNVIAGFQVYIYDWMDGATNPIIAFADQAYKMIGDEAAADRFDDYDVTVN
                     RRHIELASVMGGRLAQYKSAVKFTNQAG"
     gene            23320..23655
                     /locus_tag="VBD89_000469"
     CDS             23320..23655
                     /locus_tag="VBD89_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135025.1"
                     /translation="MTILKTIKEMVEVDVEEDIFDVQLLRYINSGISYLQRNAIPVIR
                     IDKETELEGWTDIKEEDRETVLDWLHLRCVQRFDKSLMTGSAATMEWIDGEMTNLLYQ
                     LKAIYEVKT"
     gene            23652..23957
                     /locus_tag="VBD89_000470"
     CDS             23652..23957
                     /locus_tag="VBD89_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEO7135026.1"
                     /translation="MKSSRVAIILCYDERVEVEKGVWEKKTVEKKVKAKKEKIYQRRL
                     DKAMADGQVLTARFSVRSNYVTDTLDYVKYNGKEYKVNIGTESDDGHYTIIELGELK"
     gene            23957..24304
                     /locus_tag="VBD89_000471"
     CDS             23957..24304
                     /locus_tag="VBD89_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135027.1"
                     /translation="MAKKFFTRQDIQSILETNPLKAKVFYMEREEKSSPDNVILYYRL
                     TPGSSITADDRVHMRKVTLQVSHYHKKKLDSIENLMLSNFMCEPNQLNLKQPDTDYLL
                     TTYRLEVFTNGRW"
     gene            24291..24635
                     /locus_tag="VBD89_000472"
     CDS             24291..24635
                     /locus_tag="VBD89_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO7135028.1"
                     /translation="MGGGSVKMTPLKVDIKNQVLENIKKAAQSTENDIRTKSPRRNGV
                     YEKGWTHDIIDDAAVVHNNGKEKSLSHLLENGHATKNGGFVAPREHIRPAYIKNKEKF
                     LNDMKSIKITPK"
     gene            24650..25333
                     /locus_tag="VBD89_000473"
     CDS             24650..25333
                     /locus_tag="VBD89_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7135029.1"
                     /translation="MTYQYDTREVTHGNANGFYAKIAKTDAGALDLQKPYPFTGLRST
                     SFETSQESNAYYADNVEHVRLQGKKSTEGSITTYQIPKQFMIDHLGKKLTNSTPPALI
                     DTGVNTNFIWGYAETVTDEFGAEIEEFHIWTNVKASAPKGSTSTDETSATPKEIEIPC
                     TASPNNFIVDSEKKPVSEIVWRDDSKGTVRGKFDKLFADKSPGKLIDFINEALGTTTT
                     PTTTVSGGS"
     gene            25333..25809
                     /locus_tag="VBD89_000474"
     CDS             25333..25809
                     /locus_tag="VBD89_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135030.1"
                     /translation="MIKKELSFTAFDSYGEEREHTETVRFLYSLPAIKMYEQRTGRNF
                     FDDNQKALTAYTQLALATGVNGNLSDLTDEEKIKMMPLLMEPDFMNFLTEVIPCLYGE
                     VENGRLVQNELTAETASLAPWFGDLIDIGFFSDLFYEFNRSRAKVPQDKKKPFQKL"
     gene            25995..28730
                     /locus_tag="VBD89_000475"
     CDS             25995..28730
                     /locus_tag="VBD89_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO7135031.1"
                     /translation="MTETFEGLYVKFGANTVEFDRSVKGINTALSSLKKDFNNINRQL
                     KMDPDNVDLLNRKLVNLQEQARVGAMKIAELKKQQKALGESEVGSAQWNKLQLEIAKV
                     ESQMKIVDKAMESTKKHIEDVGDPKSILNLNKELDNVAKELDIVNQKLELDTDNVELA
                     EQKMKLLGKQSELVGDKVQELKKKQAALGDEKIGTEEWRQLQNEIGQAEVEVLKIDRA
                     MDILGESSRSATGDIKEATSYLRADVMMDVADKAGQIGQKMVDAGKMTVDAWSEIDEA
                     LDTVTTKTGLTGDALAELQEIAKDIATGMPTSFQNAGDAVGELNTQFGLTGEKLKSAS
                     ELLIKYAEINETDISSSAISAKQAIEAYGLTAEDLGMVLDNVTKAAQDTGQSVDTIVQ
                     KAIDGAPQIKGLGLSFEEGAALIGKFEKSGVDSSAALSSLSKAAVIYAKDGKTLTDGL
                     NETVSAIQNSTSETEALSIASEIFGSKAAPRMVDAIQRGAFSFDDLAEAAKSSSGTVS
                     TTFDETLDPIDKLTQYSNQAKEGMAELGGKLLETVIPALEPLMGMLESSVNWFTSLNE
                     TDQQTIVILGLVTTAVMMLLDAIAPLVIAIGAIGAPVGIVVAAIVGAIAVITLIIQAI
                     MNWGAITEWLQSTWDSCAAWLSELWTNIVTTATTAWSNFTAWLSGLWSSVVSTGQSLW
                     SSFTSSLSNIFSSLITGAQSLWSSFTSTLSNLWSGLVSTGSNLFNNLSSTISGIFNGI
                     LSTASNIWNSIKSTISNAIDGAKNAVSNGVNAIKNLFNFQIKWPHIPLPHFRVSGSAN
                     PLDWLKGGLPSIGIDWYAKGGIMTKPTLFGMNGNRAMVGGEAGAEAILPLNKSTLGAI
                     GQSIANTMNTSNNINVNFSGVTIREEADLNRLANVVGNRIAEELQRKTNLRGGMA"
     gene            28727..29449
                     /locus_tag="VBD89_000476"
     CDS             28727..29449
                     /locus_tag="VBD89_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7135032.1"
                     /translation="MTKINELTIDGVKTSSFKCDVLVETRPNVIVSSSKTALLEHDGI
                     SGAVVQSNRHRGLIEKPYHITLIEPSDEEIYRFSALLNREKFWLENEQEPTIRLWCYK
                     VDSFEIGKDEFGAWVVDVTFICHPTKFFKTTDTQTLTGNGVLRVQGSALAFPKITVVG
                     QSASETSFTVGNQVIKLEKLSESLVMTNDPDNPSFKTASGKLIKWAGDFITVDTAKGQ
                     NVGVVLGAGITSLKFETVWGWA"
     gene            29450..33124
                     /locus_tag="VBD89_000477"
     CDS             29450..33124
                     /locus_tag="VBD89_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001918212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7135033.1"
                     /translation="MLFLLDKDVRTVKWNGIPLHEASSAIVKEETNSDFYLTVRYPIT
                     DSGIYQLIKEDMLIKAPTPVLGAQLFRIKKPIENDDSLDITAYHVSDDIMKRSITPVS
                     VVGQGCAMALSQMVQNAKTGLGDFSFTSDIMDSRTFNTTETKTLYSVLMDGKHSIVGT
                     WEGELVRDNFALSIKRSRGADRGVVITTHKNLKSYQRTKNSQGVVTRIHARSTFKPDG
                     AEDEVTLRVSVDSPLINSYPYINEKEYENNNAETVEDLRKWAEAKFTNEGIDKVSDAI
                     EIEAYELDGQVVNLGDTVNLKSRKHSADLYKKAIAYEFNALTEEYISITFDDKPGVGG
                     SGVSSGLSNVADAILVASATAQDVAVQRAVKNANAAFDAEFGKTKTKINDDIEIAKAK
                     VESFKSELSNRMDNQLLPLATEAKNLASQAQADLTRKEIELRAELNRQVTSTEAVKIS
                     LTNLSHNMDIIKQKALNDLRDAETRLKEADSVQQLATKRVEDKLTGLSTKLESFSVGG
                     YNYVIDGGEPKELMANFYGKTYDINPQLLERTSQATLSFSYEAESTSRLEVRLYKKMH
                     TGDTSKITIIVMPNFDLSPGKGFISQSFDLGGVMPDPRNQAWLVMRGTNANPLTLSKV
                     KLERGTVATDWNNRDETLKASFAEYKQTVDENLANLRTSTETLAGQLTSAESSIRQTS
                     ESFSNRLVSLETYKDSESNRASRYFEASKSETAKQLSALRTEVNGSYVDKSTYEENAR
                     GLTRRFESLSSGTQNYALGTERDVYLNVSGTTVYDLYSFGTTLAIKGIEMGDTVTIAF
                     NYFADSRTAFGSYELELYGHAGKIERVGEVQRTISNRGTYTASFVANNANFRANSLKI
                     RFTDSRLKFRVTTLRVTKGTIPADWSPSPDDLKAYSDTKLEQTANEIKASVTSLDHKT
                     LKQTDITMTSEGIVLRAGKTSNDVARAIGSYFKVTPDAIALFSSLIKVSGNMLVDGSV
                     TSRNLVTGAVETGHVKAGAITGVLLAAEAVTAEKLKVDQAFFNKLMANDAYLKQLFAK
                     SAFITQVQSVTISASQISGGVIKALNNAMEIQMNSGQILYYTDQAALKRVLSGYPTQF
                     VKFATGTVSGKGNAGVTVIGSNRYGTESTNDGGFVGVRAWNGSNIDSLDLVGDKIRLA
                     SSAFDNSDGWDVRTLDSGLKITPHNRAAERNSRIEVGDVWILKGNGSYSSLRDILNSF
                     NENFSKGPNADSYTYYHSGF"
     gene            33141..33371
                     /locus_tag="VBD89_000478"
     CDS             33141..33371
                     /locus_tag="VBD89_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135034.1"
                     /translation="MNQEQQLNQALRLTVNELTAQLANESTTKNLLAIQLTEVDQEKQ
                     QLTQQNAELQARVSELEGLLDEQTQPEIIEGE"
     gene            33374..33712
                     /locus_tag="VBD89_000479"
     CDS             33374..33712
                     /locus_tag="VBD89_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135035.1"
                     /translation="MTTTENNLLDLETITEPFDLATALKYMKENGELIRCKNAVNDFY
                     MYRDMQKRPVIVNGRRQFKDVETVWAFNQWGGTTPTINMADLFNLEYYIMTFDENGNP
                     DWTEPHLEDK"
     gene            33747..34157
                     /locus_tag="VBD89_000480"
     CDS             33747..34157
                     /locus_tag="VBD89_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135036.1"
                     /translation="MPPWLIDSTVVVAMVTVLGGLFSTIITTSANRKDQLIKHQYEDI
                     KEDLSGLIDKVKTIDHTTTETKKISEITKDGTLKIQRYRLFHDLTKEISQGYTTIEHF
                     RELSILFESYQLLGGNGEIEALFEKFKQLPIEED"
     gene            34160..34489
                     /locus_tag="VBD89_000481"
     CDS             34160..34489
                     /locus_tag="VBD89_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO7135037.1"
                     /translation="MTQITDIIISSAMGILTILAGIAVQAVKVYLIKKGGEKAVLITE
                     ILAKNAVNAVEQVATETGFKGADKLTSAKAQILAELQKYNIHMSDDDLTLFVESSVKQ
                     MHDAWKE"
     gene            34493..35899
                     /locus_tag="VBD89_000482"
     CDS             34493..35899
                     /locus_tag="VBD89_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO7135038.1"
                     /translation="MEINTETAIAWMSARQGKVSYSMDYRDGPNSYDCSSSVYYALRS
                     AGASSAGWAVNTEYMHDWLIKNGYELIAENVDWNAVRGDIAIWGMRGHSSGAGGHVVM
                     FIDPENIIHCNWANNGITVNNYNQTAAASGWMYCYVYRLKSGASTQGKSLDTLVKETL
                     AGNYGNGEARKAVLGNQYEAVMAVINGKTTTNQKTVDQLAQEVIAGKHGNGEARKKSL
                     GSQYDAVQKRVTELLKKQPSEPSKAQEVNKPTETKTSQTELTGQATATKEEGDLSFNG
                     TILKKAVLDKILGNCKKHDILPSYALTILHYEGLWGTSAVGKADNNWGGMTWTGQGNR
                     PSGVTVTQGSARPSNEGGHYMHYASVDDFLTDWFYLLRAGGSYKVSGAKTFSEAIKGM
                     FKVGGAVYDYAASGFDSYIVGASSRLKAIEAENGSLDKFDKTTDIGDGSKDKIDITIE
                     GIEVTINGITYELTKKPV"
     gene            36106..36291
                     /locus_tag="VBD89_000483"
     CDS             36106..36291
                     /locus_tag="VBD89_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO7135039.1"
                     /translation="MTERELKKIAKKQGFSKTNFGKGSHEVWKHPDGRTVTIPKPKEK
                     DYRPGTLNNILKVLYGE"
     gene            36352..36756
                     /locus_tag="VBD89_000484"
     CDS             36352..36756
                     /locus_tag="VBD89_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO7135040.1"
                     /translation="MKYTYLALFEVDKENGGYNISFPDFPGAFSEADSLNEAIFNARE
                     VLEIYTIMFEDEGKEFPKASSFKALASNLASDEDVIQAISVDTELVRERERSKIVNKT
                     VTLPSWLVEVGKENKVNFSQLLQKAIREELQV"
     gene            complement(36970..37040)
                     /locus_tag="VBD89_000485"
     tRNA            complement(36970..37040)
                     /locus_tag="VBD89_000485"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37006..37008),aa:Cys,seq:gca)
     gene            complement(37174..38706)
                     /gene="ahpF"
                     /locus_tag="VBD89_000486"
     CDS             complement(37174..38706)
                     /gene="ahpF"
                     /locus_tag="VBD89_000486"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO7135041.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQTDLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGQESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKNVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(38724..39284)
                     /gene="ahpC"
                     /locus_tag="VBD89_000487"
     CDS             complement(38724..39284)
                     /gene="ahpC"
                     /locus_tag="VBD89_000487"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO7135042.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            39561..40331
                     /gene="rpsB"
                     /locus_tag="VBD89_000488"
     CDS             39561..40331
                     /gene="rpsB"
                     /locus_tag="VBD89_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO7135043.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            40425..41465
                     /locus_tag="VBD89_000489"
     CDS             40425..41465
                     /locus_tag="VBD89_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO7135044.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(41624..42085)
                     /locus_tag="VBD89_000490"
     CDS             complement(41624..42085)
                     /locus_tag="VBD89_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO7135045.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            42258..42722
                     /locus_tag="VBD89_000491"
     CDS             42258..42722
                     /locus_tag="VBD89_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO7135046.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            42719..45166
                     /locus_tag="VBD89_000492"
     CDS             42719..45166
                     /locus_tag="VBD89_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7135047.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTIGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKHKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
     gene            complement(45386..45877)
                     /locus_tag="VBD89_000493"
     CDS             complement(45386..45877)
                     /locus_tag="VBD89_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO7135048.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARASYDWTCELSIYLDKDVRSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
     gene            complement(45891..>46361)
                     /locus_tag="VBD89_000494"
     CDS             complement(45891..>46361)
                     /locus_tag="VBD89_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO7135049.1"
                     /translation="LNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNFKNGNVTKTEL
                     DIYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDY
                     YKQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
BASE COUNT        14931 a         8265 c         9835 g        13330 t
ORIGIN      
        1 cttaagtaaa gtaaagaaga aaaagaagaa attaataata cttgaaaaag aaagatgtcg
       61 ctgtcgattg gagagagaag atactaaaaa aatcgatgat aaaattgaga aattgaagaa
      121 agaattgata gatgttatga aggaggaata atatggaaga aactattcta atagtcagct
      181 ttctcctgtt tttgatttta tctaatgtta tcaataggat atttcctaaa ttaccattac
      241 catttatcca gttggtcttt gggattctct ctggcttagt ttttcataag tcacaagtcc
      301 atatcgatcc tgagttattt cttgcctttg ttattgctcc tttgaacttt agggaaggtc
      361 aagaaagtga tattggtagt tttgtaaagt atcggactat tattctttac ttaatcttac
      421 caacggtatt tttaacagct attgtcgttg gttatgtagc agggcacctt ttgccagtta
      481 gtttaccact tgcggcatgc tttgcgcttg gagcagcctt aggcccaaca gatgccgttg
      541 cctttatatc gattgctaag aggtttcaat ttcctaaacg agcagagaat atcctcaaat
      601 tggaaggtct tttaaatgac gctagtggtc tagtttcctt ccagtttgct ttaacagccc
      661 ttgtaacagg ttatttctcc ttagcaaaag ctagtctcaa gttagcattg gccataatgg
      721 gtggtttttt aattggcttg ctttttgctt ttctaatgag gttatgtctc actgtcctag
      781 agaaatttga tgctgctgat gttacagggg ctctactttt ggaattaacc ttaccttttg
      841 ttgcttattt cgttgctgat ttgttgggat tttcagctat tattgcggtg gtagtggcag
      901 gagtaatgca agcaaatcgt ttgaaaaaag tgactctctt tgatgctcaa gtagatcgcg
      961 ttacctctgt tatttgggaa accttaaatt ttattttaaa tggtttagtt ttcttaatat
     1021 ttggaagaga actgatacgt atcattggtc ctcttttaac aagtaacgct tatagtaatt
     1081 ttgatcttat tagtattgtt gttttagtta cttgcacact ttttctagtt cgctttctag
     1141 cagtttctcg cttttatgct tggagatctt tcaaatacca taaatcattt aaaaagtatt
     1201 ggagagagat tcaactgttg actttttcag gagttaaagg aagtgtttcc atagcaacaa
     1261 tactcttatt acccaagcac tcagtcatag gagagttggg gtatagtttg attcttttca
     1321 ctgttggtgc agtaacgtta atgagttttt taacgggact tttagtatta ccaaagttag
     1381 cgcctccctt acaggtgaaa gatgattatt taatacgtct atcgatttta acaaaagtat
     1441 tatctgtctt agaagaagac ggtaagagtt ctgaaaatca agcttcattt tatgcagtta
     1501 tagataatta taatagccgt attcgtcatc tcattttaga acaagaatct agtgacatta
     1561 agaaggactt agctgaattg caactgatga tgcttagtat agaaagcgac gggcttgaag
     1621 ctgcttatcg ttatggcaat atttctatca aagaatatcg tatttaccag cgttatttaa
     1681 aatacttaga acgtcaagtt aatcgtagct ttgtgtcaaa tatcacttat agtttaacga
     1741 ttcttatgcg tgttattcgt ggtattgttc atgagttttt aacatttgga caaggaatac
     1801 gaaatcggaa aaaacatttc aaaaacagga aacggttgtt agcagaagtc aatcgtcagc
     1861 acttaacaga tttgtactta tcaaataatg acctcatttt agaaacgcta gaagatatgg
     1921 aaggttatta taatgcaact ttgatagatt tcttaaaaaa tgaacgcatt cgagacgcag
     1981 aattaattaa atcaggttta tttgtagaac gtgtcattgc acgcataacg ccagatattt
     2041 taaatgaaac cctaagagga tattacttgg aacgcagaac aatctctgaa tttgaaaggg
     2101 acggtttaat tagttctaaa gagtctcaaa ttttaaggga taatgtcaat gaattagaaa
     2161 gctattcatt aagagatagt caatcttcta taccttatca attattggat ttaaaaaaat
     2221 aattctgata acaaaaaaat caacgaacaa tcgttgattt ttttgttatc tttggataaa
     2281 atcttttata tttaaggcgg tagacggatt tgaaaacctt ggtttatcaa ggtttttggt
     2341 tgtttcgccc caaattcgcc ccaaattatg cgactaaaaa aatatttttg attttctcaa
     2401 aatttctctc agccagtgct tccatctgat gtgtataaac tttcagagta atatctggac
     2461 tttcatgtcc aaggagctta gaaattgtca caatgtcaac cccgttcaaa atcaaaaacg
     2521 aagcgtatgt gtgccgtaat gagtgatttc tgactttcct acccacaatt ttcttaatca
     2581 gtttattgca agctgaatta gatacaccaa agcacactct attcttgata ttagcttgcc
     2641 aatgattttt cttatattct cgaagaatat ctattgtatt tgaatcaatt ggaacttttc
     2701 tttttgatga ctcattcttc agatctgcaa aatcttgagt attcgagtaa tcaaaactct
     2761 tgtttatatc caaaatgtca tttttgaagt ctatatcgct ccaggttagt cccatgacct
     2821 ctgagaaccg caaaccagtg acagaaagaa ggtagagagt gaaataggat acatactgga
     2881 tattctctct tgtgagggat aaaagagcct tatactcact ttcttctaag aagtcatttt
     2941 cctcagctct tgattcaatt tgtgatttaa cctttgcatc atcagcaaag ttaaaatgaa
     3001 taacttgttc cctcacagct actttcatag ctcccttgat ttggtagtgg aacttctcaa
     3061 gagtttcttg agcatatttc tccccaaatt catttagccg tttttgataa tagagtggtg
     3121 ttatatcctt aacctttata tctttaaaat aggttttgat gtgtctaaag ttctttgtgt
     3181 aggtttccca ggtcttatcc tttacataag gacgtttata aacttctgac caagttttga
     3241 caaaatcata tagagtaact tctccatcgg tcaggatatt ttgagatagt tttttctcaa
     3301 tatccctagc agctgcttga gcaagttttt tggttttgaa accactctta gacttttgac
     3361 ggaacttacc gctctcatct ttatatgaaa tgcgatattc ccagccatta tcacgttttc
     3421 gataataagc catttgtttt tcacctcatt ttttgataaa atgggtatag taaagagacc
     3481 tactgctaaa gcaggtttct cactatccac aattgcctta cgcttgcacc gaccaaagtt
     3541 gagcgtaggg ctttttttat tttttaactt tatttttcaa cgcattctcg atagcatttt
     3601 taagctcaag aatagctatt ttctcatctt tactgaaggt tactgtattt tcgtctttta
     3661 ctgcatcgaa aacaccgccc ttagtatcag atgaaccagg ataaaccaat tgtagatagc
     3721 caacagttga actaggttcc ttgagttgat aagctgtaat ttctgataat agaattgact
     3781 tttctccatc taagccatgt agcaacatat ttgacatgtt gctttttctt gcgattctga
     3841 tgaagtaatc atcaatacgt acaattgttt ttgatttttt gaattcaaaa acacgttcgt
     3901 taggctctga tgtaaagagt tctacttcaa tttctttatc ttttccgcca aataatgcca
     3961 ttattgttgt ctccttttgt ttttattgta accgtaaaat gttaaaaatc tgccctccac
     4021 cttgacggta gtttaaaact aacttgaggg cgtagctttc agcatactgc aagttataga
     4081 tgagatagtt catcagtctg ttgtgtagtg ctggtttgct aatatctgtt tcacgttgaa
     4141 tatcttcaaa gctctctcta gttgtgaggg cttttttgag tcgttcatct gttaggtaga
     4201 ggattgaagc cactgtatca gcttctttct caattggaat cagctctttc gggtacttct
     4261 cactagcgtt tttactcatg agagacatat agattggctg attttctgtt tgattaaggt
     4321 gacagtgtat gtgactaagt tcgtgtaaaa tcgtgaagat aacacgtcct ttggtgtttg
     4381 tatgttgatt gatatagatg atgtagcgtc caaggtctag gtcagggaca gtcaatccag
     4441 cacagctttc gcagagtacc ctatcagtat aagtaacggt tctatttgat accagccctc
     4501 tgtacttgat gtctttatct gtgggatgat aatcaggtaa ttctggataa cgttctttca
     4561 tctcatcgta atcaagaaag ttaaagagaa taggatagtt ttgttcaaaa tgtttaatga
     4621 catcttgata actaacgttc tctttcattt tatcaattga gcttattatt tgataagcct
     4681 tattgtgata gtcaaaatac tgttcccttg atagcggtgt gtatttcaat aagtcaccta
     4741 cttccagtta gaatcatctt gaatcaattg tctagctgtc ttcattagac cttcaagagc
     4801 agaattgaac cgctcttttt cagaagctga aagtccttct gtttccttac gaaaaagaac
     4861 aatagcttgc tgagttagat catcaactgc agtattatct tggccatcgg caatcttagg
     4921 attttctgtt ctaccgagaa ggtagtcagt gctgacattg aaataatctg ctatttttga
     4981 gacacgctca acatttggtg tagatttttt catgttgtat atggtatttc taggaaaatc
     5041 caactgttct tcaagttgat tgagtgaaat accttgcttg tcagccaatt ccttgatctt
     5101 ttcaaatgta aaaaacattg atttatcaac ctttctaagg catgacaaaa aatatttaat
     5161 aaatttacta caaaactgtt gacaggtttt aataaattta ctacaatatc gtttgtaagc
     5221 taagtcgtta gcgaacaaga caagtaaaaa ataaagccta aacaactgat tggcgtcggt
     5281 tttatgggtt aagccttagc tttttagcag gtcttttctc tatgctttga ttttaataaa
     5341 tttatttatt gttgtcaaga agttcgctaa ctttttagca aaaataataa aaggagggta
     5401 gatgaatggg aatcggttca atgacagtga cagttaaagt tactaacatg gataaattta
     5461 ttgaacttag taaagagttc aataaaaaag ctcgcgagct agaagaacta gctcacgaac
     5521 tgaagtcatt tgattttgaa ggcgaagttg tatcaactga tagcaactag ctcaaatgat
     5581 tcatcatctg aaaaaagata aacagggtat tcttctttag ttgcaaaatt gaagaaatca
     5641 tatccaaaat tatctttttt gatgtgggaa tttttttcgg agagagagtc aagattgacc
     5701 tgagatagta gcttaaagat ttgcacacga gcttgttcag catcgttaac tctttgcaag
     5761 gctctcaagt gctcttcgct agggaaagct gctatcaaat cagctctgtc atctatgtaa
     5821 gttctagtgt catcaagata gccatatttt agattatagt ctacaaatga ccgctgttca
     5881 tattcttgat aggtttcaaa gctgacaagg ttacctttgt aacgatagac gagaacgtca
     5941 tcttgtaact cttcttcgaa atttgtgatg ccaattagaa atttatggca tgcctctatg
     6001 atacgaattt gattcaaatt catggcttga cctcctttca taaaagattt caagtcaatt
     6061 ataccaaact agaaaggaaa aatatgagtc aacaacagaa aaaatggatt cagctagtga
     6121 aagataagct gaatgaagag caaatgacac agacacatct agcccgtgct tgtggagtcg
     6181 caaaagcgac aatctcagag ctactaaaat acggaaaagg tagtgtccgt ctgaaaaata
     6241 aagtctcaga cattcttcac attgatgaaa gctggacgga cttggaggaa gattgatgcc
     6301 gataacaaga gaaatgacaa tcactgaaat caatgttctc aatgctatca agaatagcgc
     6361 tacttatgat cttccgattc aagccagaga gctaaggcag cagctaggac ttagcaaacg
     6421 atcacttgaa gcagtcattg agaacctgag agtgatttac aagcaaccaa tcgttgccaa
     6481 aaaaaaacag cctagcggat actatcttcc aagaaatgaa gaagaaagaa acgacggctt
     6541 agccccatac aaaagacaga tattgactga gcaaaataat cttgctgcta ttatgtctgt
     6601 caatctggaa gaatattggg caacagaaaa agcctgacgg caatcaggct cacaaaatat
     6661 tattacaaga ggattatatc acaatgaacg aagtttggaa agatatacca attgttaaag
     6721 gatattatca agtttccaat cttggtcgtg ttcggtctat tggtagaaca gtcaatgcaa
     6781 aacagagaac aagaaaaaca aaaggacgca tcttaaaaca gagcttatct tcaggctatg
     6841 caatagtgac actttcagtc aatggattaa ggaagtcgat aagagttcat agactagtag
     6901 ctgaagcatt tataccaaat ccaatcaata agcgaacgat aaatcatatt gatgaaaata
     6961 aacttaataa tagagtagat aatttagaat gggcgacaga taaggaaaat gctaatcatg
     7021 ggaatagaac aacgaaatca tctttaggtc gttgtaaacc tgtagaacaa tttacacttg
     7081 aaggggaatt cataaacacg tttgactcaa taaaatctgc atcaatgaaa acaggtatct
     7141 cgtcacaaag aataacagca acagcaatgg ggcaccaaaa acagactcac ggatataaat
     7201 ggaggtacgc ataatgaatg atctaatgat tcaaatgctt gaccagttcg aagctggtct
     7261 aatggacaga acgatgaaag taatgcacgt tgtgacagac gaaaagtata gatttccttt
     7321 agaactcaac aagtctcaat gcgctttaat gctcttaggt actaaagata ctgggacatt
     7381 tgatgaacgc tttaactgtc ataaggattt tccgaggatt ccaaatgctc gtgagaaata
     7441 ccctcgtgat gaagtcattg aatggtatca caagaattgg caaagaactg ttatctaaga
     7501 aagaacaaaa ggaactatat catgaacaat ttacaaatta tcgcagtagg cacacttatt
     7561 tcagtaaccc tgattgagtc tcttatctca aatatcaagc tcagaaaagc tctcaagaac
     7621 aaacagacag ctgggactgt cattgctcaa tatatcccac aacgaggctt cattgactac
     7681 aagactggaa gacgtgttga cattgaccct gaaacaaaga aagaagtctt tgttgactga
     7741 ggaaagctat ggatgaatta aaagtattgc ctcacgatat ccaggctgag cagtcggttc
     7801 ttggttcaat ttttatcaaa cctgaaaaaa tgattgaggt ggctgagtat ctgaaaccaa
     7861 atgactttta caggccagct cacaagattc tatttaaggc catggtgagc cttgctgatc
     7921 gtggcgaggc catcgatatt gtcacaatca agtcaacgct ggaaagtacg gatgaacttg
     7981 gcatggttgg cggtatcagc tacatcgctg agattgttaa tgcagtgcca accagctcac
     8041 acgctgagca ttatgccaag attgttgcta aaaaagcaca gctacggtct atcattgaca
     8101 acttgtctga ctcaattggc aatgcctatg atgaggacat ggatattgat gagattatcg
     8161 caaaagctga gcgatcactt atcgaagtta gtcaagccag taacaagagt agtttcagac
     8221 ctatccatga tgtccttttg gaaaatcatt caaagattga ggaacgctca aacaatacca
     8281 gtcaaatcac tggtattgaa acaggatttt atgactttga caagctgata acaggcttgc
     8341 atgaagacca gctaattgtc ttagcggcac ggccagcgat ggggaagaca gccctagctc
     8401 ttaacatagc tcagaatgtg gcaactaagt ccaataaggc tgtggccgtc ttctcgcttg
     8461 aaatgggggc tgaaagcctt gttgagcgga tgttgtcagc tgaaggaaca atcatcaacc
     8521 atcatattag aacaggaaat ttgacggtta atgagtggca acgtcttatc tatgcacagg
     8581 gtcagcttgc tgaagctcca atctttatag atgacacagc tggagtcaag attactgaca
     8641 tcagagcaag agctaggagg ctatctcaag agactgacgg acttggattg atagtcattg
     8701 actatcttca actcattcaa gggtcacggt ctgacaaccg acagcaagag gtatctgaga
     8761 tttctaggca gttaaagatt attgccaagg aattaaaagt gccagtcatt gcactatcac
     8821 agcttagccg tggggttgag caaagaaacg acaaaaggcc tatcatgtcc gacttaaggg
     8881 aatcaggcag cattgagcaa gacgctgata ttgtcgcctt cctctatcgt gatgcttatt
     8941 accaagacaa aaaagaaggt cagccagaaa acgacatcac tgagctgatc atcagaaaga
     9001 acagacacgg aaaccttggc acagtcaagc tgtatttcca caaggaatat accaagtttt
     9061 ctagcgttga ggaggaatga aatgaaaaaa tttgaccact atttcagcaa ccatattgct
     9121 gtaaaaaacg aatctgatac agtcaccatt gttgactatt acaatccaga aaagggctat
     9181 gagtataact tacgttacat tttcgataga aaaaattcat cccttgctat cacaggagac
     9241 tttggggaat tagttgcacg aaatttctac aatatgggtg aatgggacac attctacaat
     9301 cactatactg gtcatctggg gtattttctt gaaaagttac tatgttcaag taggccagtt
     9361 cattactatg atactgacaa ggctaaaaga gaaattctca atgaattctt tgatggtgct
     9421 gattatgatg atttagattt tgatgatgaa ttcctacttg atcaattatt tgacaatttt
     9481 gacgaagata aaggatttac ttatattctt ccagaggctt ttgaacgttt tagcgaagat
     9541 atggaagaat atgaggtcta tgaatttcta tcaagggctg gccgatacgt caatgatgtc
     9601 tttgaacttt acttagacgc ttataagaga gcttatgagt atctaacaaa ggaggttgat
     9661 gatggcacaa cgcagaatgt ttagtagaaa aatcactgag actgaccgtt ttttggaaat
     9721 gccactatca tcccaagctc tctatttcca tttgaacatg ggagctgatg atgagggctt
     9781 tatagataag gcaaagacga ttcagagaac tattggcgct agtgatgatg atatgaaatt
     9841 actgattgca aaagggttct tgattccctt tgatagtggt gtagtcgtta tccgtcattg
     9901 gcggattcac aactatatcc agtctgaccg ctttcaatca accttatacc agtcagaaaa
     9961 ggctcagcta gagtatgaca agtcaaaaac atctagtctt aaacctattg gaaattgtat
    10021 acaaaatgta tccaaaatgg agacacaggt taggttaagt aagggtagct tagataagga
    10081 tagcttaact acctatccta cagtttcaga caatgaggaa gaagatattc catacaagga
    10141 aattatttct tacttgaatg aaaaagctaa tagaaattac agacctaaca ttcagaaaaa
    10201 caaaactctt atcaaggcta gatggtcaga aggattcagg ttagatgatt tcaaacatgt
    10261 gattgatacc acggtcaagg attggtcagg cactaagtat gaaaagtatc tcagacctga
    10321 aacgctcttt ggttctaagt ttgaaggcta tttgaatcaa gcaccacgaa tcaagacgga
    10381 aacaattgac ggaaggttgg gcttttagat gaatcctttt aagaattttg aaactaggca
    10441 agtcttggaa gaaacctgcg aggttcacgg ttgccagctc tggctgacta aagtaccgat
    10501 taagggacgg cttgaagaac tcaagcagtg tccagagtgt accaaggccg ctatcaacat
    10561 ctttgaaaat aagttgaaca gtcagagcaa aatcaacagc aagcttgctg atacatatgc
    10621 tgtctttgaa agagatagct tggtatcaga caagctaaga ggcaagagcc ttgagaacta
    10681 tgaaatcaag gatgatattg accagcaagc tatcaattat gctaagcgta tggaacagtt
    10741 ctaccgacag gatagaacag gcaatgccat tgtaactggc ccttctggtg ttggaaaaag
    10801 tcatctgact tacggcttgg ccaagtttat gaacgaacaa ttcaaagcct acgaatcacc
    10861 aaaatcagtc ctcttcatct cactggtgag cctttttacc aaaatcagag aaagcttcaa
    10921 agctgacaat gggtaccgac aggctgagat gattgagctg ctaactagtg tggactacct
    10981 ctttctggac gatctaggca aggagagtcg caaaggcgac agccagaaca atgagtggac
    11041 tcatcagatt ctctatgaga ttttggacaa tcggagtaac acaatcatca acacgaatct
    11101 gagcagtaag gagattaagg ccttatatgc tgacaattac ggtaatggtg ctctttctag
    11161 tcggattctt gagggagtga ctggcaatag ctttgcttac cctaaggata tggaagatag
    11221 gaggtattga tgagctataa agccacattc atgctatatg gttacaaagc tgttccgatt
    11281 ggtctatttg atacaaagga gttggccgct caagcaattg ttgaccacat cattgaaaac
    11341 tctggaatac ctagacccag ggtagttgcc actatgaaaa tccgaagcaa ggaaatcaga
    11401 attgattatg gttctaaaac ttgctactac ttgatcactt taattaataa taaaacggag
    11461 gaaaaacaac atgactaaaa caatgacact tgaagaaaag gttgaacagt ggtttattga
    11521 ccgtaactta catgaggcca atcctgttaa gcagtttcag aagatgatgg aggaaactgg
    11581 ggagctttac tctggtattg ctaaggacaa gagtgaaatt atctatgatt cgcttggaga
    11641 tatgcaagtt gtcctgattg gcattgaaca acaaatcaag aatggcgctc agattgaagc
    11701 aagtccacaa gatatggagt tgttgctatt ggcttcaagt ttgggggaaa tggcacagaa
    11761 actctacaag catatcttcc acaatgaaac aaaaacaccg cttatccgtc cagaattaag
    11821 cttgcttcac tctactattc atgcaatagc tatccacaac ttgacaactg ctgatgactg
    11881 cctatcaatc gcttatgaag aaatcaaaga cagaaaaggc aagttgatca atggcatttg
    11941 ggttaaggag gaggatttga aatgaaaaac atgaattgga aagcattctt caacaatctc
    12001 aaggcctttg caagtgtcat catcattact gtatttgttc ttggattcaa ctatctgttt
    12061 tatgagattg gctaccagaa aggccatcag gaagctgaca gggtgattat ctatgtcgct
    12121 gataatgctg gcgctgagat gtttggcaaa atcactgata aagaaatcat tgaaggccgt
    12181 cacacggtta cagctggcgc ttatggaaaa tttctggtca ctgaggaaca atacaatgaa
    12241 atcaccgttg gtgatgacat accagactac ctgaaaggac gtggaaacta atgacaaaat
    12301 gtaattttga agcaggacac aaaccaattg acacaattgg acaactagcc aaagaagtcc
    12361 ttgatgggaa atatggcgat ggtgaggaac gaagaagaaa tctaggctgt ttatacgatc
    12421 tagttcaaac aagagttaat tatatggttc atgaacgtga cagcaagttc acaggtatca
    12481 agatttacga caatgtgaac aaacctagcc attatcaagg aaagaatggc atggaagcca
    12541 tcgatgttgt cagaaatttc attggacagc tcacaggagt atctgctttc tactggggaa
    12601 actcaatgaa gtacctgtta cgcttccagg gcaagaacgg ccttgaagac ctcaaaaaag
    12661 caagaaagaa ccttgactgg ttgattgaag aaatggagaa agagaaatga aaaaatcaat
    12721 aggaatcctt gaatttaaag aatttgatgc ttttgttggt ggaaaggtta tgcctgtcta
    12781 tcttcatagt gctaacccaa acgtgccaac gactaataaa aatgaggcta tgagggttac
    12841 caaaaagaga tacagaaaag agttctctga caagtacatt ttcagaaagg ttggtgaggt
    12901 tgatgatcaa taatgttgta cttattggcc gcttgacaag agatgttgag ctacgttaca
    12961 cgccgtcaaa catcgcaaac gctactttta acctagcagt caatcgaaat ttcaagaatg
    13021 ctgctggtga tcgtgaagct gatttcatca actgtgtgat gtggcgacaa caagctgaaa
    13081 acttggccaa ttggacgaaa aaaggaatgc tgattggtat tactggacga atccagacaa
    13141 gaagctacga aaatcagcaa ggtcagcgta tctatgtgac tgaggttgtt gctgacagct
    13201 tccagattct tgaaaagcgt gataattcaa cgaaccaggc aagcatggat gaccaattgc
    13261 caccatcatt tggaaatagt cagcctatgg atatttcaga tgatgatcta ccgttttgag
    13321 tgtttcgcta agcttctctc aatctaacaa gtgaaagtcc tgtcctaaca attaaccagt
    13381 tcggttaggt agctatggca tacgtatgcg gagtaatcca aatgcggaag caatgccgac
    13441 taacgttatc cccagtaggc tgatattcat tggtcttatc tcagtccgtg ggagcgagca
    13501 aacctacagt ccgtaacgaa agtgaacttc cgctaagtgt cgttaaggtc attccaatga
    13561 gtataactga gagagttgga aatagagttg tcgagccttt agaatggggg cgaagactgg
    13621 aattgttgtc agttctaact ggtatatact ggcaataaag ctctcggcat atagaagatg
    13681 gaatgtaggt aaagatgagc gaggaacgaa agagaccctt ttctatcata catatcccat
    13741 gtatgtaagg ttagatataa ggaaaacacc gaagttctaa ttgacgtagg aatagggagt
    13801 cggagaggtt catagtaccg ataatcatac gagacaacaa aactcgatgt agagaaggag
    13861 ccttgccttt atcggtctta ccgatgaaag aggaactaga tgattgctca gaaagctata
    13921 aacatctttg aaaaggttca agtatttcaa cggaagatat atctatcgac caaggcagat
    13981 aataagcgaa agtttggtgt tttatacgac aaagtatatc gtaaagatat cctgaaagtt
    14041 gcgtggttct atgtaaaaag gaataaaggt tcggcaggca ttgatgactt cacgattgaa
    14101 gaaattgaag cctacggtgt acagaaattt cttgatgaaa tagaagacca gttgagaaac
    14161 aagaaatatc aacctaaggc agtaaagcga gtttatattc caaaagcgaa tggtaaaaag
    14221 agaccgttgg gaattccgac agtccgagat agagttgttc aaacagctgt gaaaatagtt
    14281 atagaaccga tattcgaagc ggactttcaa gaattttctt atggttttag acctaagcga
    14341 agtgctaacc aagctataag agagatatat aaatatctca attatggttg tgagtgggtt
    14401 attgatgccg acttaaaagg ttactttgac acgatacctc atgataagtt actactttta
    14461 gttaaggaac gagtaactga taagtctatt atcaagttac ttagtttatg gctagaagca
    14521 ggtatcatgg aagacaatca agtgagaagt aatattttag gtactccaca aggaggtgtc
    14581 atatcaccgt tgttagcgaa tatctaccta aatgctttag acagatattg gaagaacaat
    14641 cggttagaag gaagagggca tgatgcccac ttgatacgtt atgcggatga ctttgttatt
    14701 ctatgctcaa ataatccgaa gaaatattat cagtatgcga aacagcgtat tgataagcta
    14761 ggattaacat tgaacgaaga aaagacaaga attgtgcatg cgacagaggg atttgatttt
    14821 ctaggctata cgcttaggaa atcaaaatct cacaaaagtg gtaagtataa aacctactac
    14881 tatccttcaa gaaaatctat gaaatcaata aaaggcaaag taaaagatgt tatccaaaca
    14941 ggacaacacc tgaaccttcc tgatgtcatg gaaagattga atccaatgtt gagaggatgg
    15001 gctaattatt tcaaagctgg gaactctaag caacacttca agagtataga taactatgtc
    15061 atatataatc taacaattat gcttagaaag aagcacaaga agtctggaaa gggatggagg
    15121 gaacatccgc cgtcatggta ctataactac tttggactgg tttgtttaag gaagttgagt
    15181 accaatatca atgatgatag tcagagatat ggtagataac ttgtgaatgg ctataacgtc
    15241 gaagaaagaa gatattggga aagccgtgta agggaaaacc ttacgcacgg tttgacgagg
    15301 ggtttctgag gagatggctc aaatcagcga cctactctac aggaggtgct tatgaacgaa
    15361 tataagaaac ccacctacat cgtcattcaa gaggcaatgg ctgagcgtat tagatttctt
    15421 gaagatgaat tatataacag agcatacaaa gacattgaac ggctagaagt tgagaatgac
    15481 cgtctgaagg ttagatgcct tgatttgcag ttggaaaatg ctgactatgt ctgggatgac
    15541 atgtgtcgct ctgctattgc tcgtgcaaaa tcagaaaatc gaagaccaca cagaagatgg
    15601 aggggaagat gaagttaagg cttgaagaac taagaaacaa acttggcctc agtcgcagag
    15661 atgtgcatga agccactggc atttctatga acacgctgct acattatgaa aaaggcggta
    15721 caccatctat tggtcaggtt gaaaatatcg ctaaggctta caatatcaac ccagcctggt
    15781 tgcttggttg gattgatgaa ggccagcaac cacaaccact tgaaaccgtt gttgaaaaga
    15841 ttatctatgt ggaaaaggaa ggctgtagat taccgccata ttggaacaat gataataatg
    15901 gcagaatcat caagtggaaa gaatcaagaa gagcattcca aaggagaaca atttgacaaa
    15961 gaaaacagca agtaagacaa gacgtgattt ccttgagttt gagcttgagg ctaagtatct
    16021 aaaaattgat aaacttattg ggcagcgccg tcatgaactg gaaagaatct atgcagtcaa
    16081 aaacctgaca atgccaggaa tcgatgattc aggagctagt cgcagtggaa cttcaactaa
    16141 tacttctgaa aatctagctg ttgcttatgc cagtgaccca atgattctga aactggaaga
    16201 gttccaaaat gcgatatcag aattactaga ggtacttgaa cccgatgata agaaaatttt
    16261 ccatctgagg tggggagaac atactggata tgattggatt caagtttggc acatcatgga
    16321 aaatggcgag acaggttatc tttacagaca tagcaagcag atttatagaa gacgtgaagt
    16381 catccttgat acacttgcaa aattattgtt catgtaactt gtcaaaaaaa catatagaat
    16441 tgacagaaac aatctgatag attgatagtg tcgctaagca ccgagaaatc cttggtgctt
    16501 tatttttttg agaaaggagc aaagcgatga atattgttga accgctacga gacaaagacg
    16561 atatacaagc catgaaggac tatctatcat cttggaatga aaagtattac atgctatttc
    16621 ttttgggcat caatacaggt ttccgtgttg gagatattct caagctaaag gtcaaagatg
    16681 tccaaggctg gcacatcaaa gttagggaac aaaagactgg taagtataaa agtattaaga
    16741 tgacaaggcc actcaaaaac gaattgaggg aatttgtcaa agacaaggaa cttcatgagt
    16801 atctatttca gagtcgtgtc ggaaagaaca aggcgctcag ttataagacg gtttactggt
    16861 ttcttaaaag agctgctgaa gacctcggca ttgacaatgt tggaactcat acaatgcgaa
    16921 aaacctttgg ctatcattac tacaagaagt acaagaacgt tgctgacttg atgtcactat
    16981 tcaatcattc aagcccagca gttacactaa tctacatttg tgtaaggcaa gatgagcttg
    17041 atactaagat gagtaatttt agcctctaat atttttttga ttttttcaac tatccataac
    17101 gaggaagttt ctagtctata ttttgaagtg agcttaaagc cttgtcctaa ttagttttct
    17161 agtgtgaaac aaaattggat aaaatataag atatagatag ttcagcatgg ttattttaca
    17221 taatttcagc catagaaaaa taatcttgtc aaaaaaatgg gtattattga caaaaacaat
    17281 ctgatatatt tgtaacatga gaaaaatcca gagagcaagc ttaggcatgt tctttttttt
    17341 gttgggggtg acgatgccat gaaagatgaa aatgcatttg atggaattag gcttgaagct
    17401 ggtagcactt tagaaatcta tctcacgaag aacgacttag aacatattgc aaatggatat
    17461 gaggtgactt tagatattaa gcctaacgag actgtaaata aaatcgtaat aaagccaagt
    17521 tttgtaaata atattctaaa tccgttaatc aactatgaca aaagaatagt gagtgaagca
    17581 gatttaaagt ttagagacat ttcaagagag gttgcaagag atagttttgc attggggtca
    17641 atgtagtggg ctatagaaac cctaaacact ctgactggtt cagagcttgg cagattaaat
    17701 tctacaactc gaaaccttgg agaactctga gaaataaaat cagaaaagct aagcgtatgc
    17761 gttgtgacat gtgcggacgt ttgattcacg gtaagagtat tgttgaccac atcatagaga
    17821 ttgacgaaac taattatcaa gatgaatcta tcactctcaa cgaagagaac tgtcagttac
    17881 tttgtcttga atgtcacaac acaaaaactt ttcaaagtaa aataaattta aatttagata
    17941 atcggaatat aaatttattt ttgatttttt tatttttgaa tttttgtgga ctccccccat
    18001 tttgaatttt gacagcgcca aaataataac ggtgtcaatc ctcttgtgta cctctccccc
    18061 aaaattgacg aaaattgata caagaaagga gcatgatttt gaaaatcaat gaagttttag
    18121 aaaagctagg aataagtcgt gctaccctta ccaggtatcg aaaaaagctg ggcatatttg
    18181 aagaaactag gtcaaatatc accaaaagtc agttcaaaga gttggaaaag cttgccaatc
    18241 aacggcaaaa gtacacaagg caggaacgtg ttgagctatc tcgtaagact ttcaagctga
    18301 ttccaaaaga aaaaatgctt gaaatcagtg ataatgattc agttgtgttg aaaaatctca
    18361 aaactcaata caatcacaat caaaaagtga ttgaaaactt ccagcttgaa atcaataagg
    18421 tcatcaatga cggtgagcta cctgataagt acctacttga tggaatggaa aagtatcaaa
    18481 agcttaacat gcagatcatg tcaacgattg aaaagcaaag tccacagggt gacagtctca
    18541 aagaaatgat tcaggagaag ttagcacgtt atggttgaga tgagatattt tgataagtat
    18601 gctcagctca tctatactgg taagattcgt atttgtaagc tcacaatgaa atcaattaga
    18661 cgtgttgagc gatacaaaga gcaatacctc ttcaaacagg aggaagctga caaacggatt
    18721 gagttcattg aggaagagtg cagcaatact aaaggccttg ctggtaagtt acgcttagca
    18781 ttaccacaaa aggtttggtt agaaacaacg tggggctttt atcacacggt tgaggttact
    18841 aagaccaatc ctgatacctt ggaagaatac acagattatg aagaaaggcg tctcattcat
    18901 gaggtgccta ttattgtgcc tcgtggcaca ggtaagacta ctcttggttc tgctattgct
    18961 gaggttggtc aaatcattga cggtgagtgg ggtgctgata ttcagcttct tgcttacagt
    19021 cgtgaacagg ctggctattt gttcaatgcc tcaagggcga tgttgtcgaa tgaagaaagc
    19081 ttgctgcact atatgcgtga ggctgacatc ctacggtcaa ccaagcaagg tatcttgtat
    19141 gaaacaacta acagtcttat gtctatcaag acttctgact atgaaagcct tgacggtact
    19201 aatgctcact acaatatctt tgatgaggtg cacacttatg atgatgactt catcaaggtt
    19261 gtgaatgatg gttccagccg taagcgtaag aattggataa cctggtacat ttccacaaat
    19321 ggaactaagc gtgacaagct ctttgataag tattacaaca tctgggtaga tatccttgat
    19381 gacaagatta tcaatgattc tgtcatgcct tggatttatc agttggacga tgtgtcagag
    19441 attcatgacc ctgatatgtg gcagaaagct atgccattac ttggtatcac gacagagaaa
    19501 gaaaccatcg ctcgtgatat tgagatgagc aagaatgatc cagcacaaca agctgagctg
    19561 atggctaaga ctttcaatct tcctgtcaac aactatcttg cttacttcag caatgaagag
    19621 tgtaaaggtt ggtcagataa gtttgatgag agtttgtttg tcggagatga tgaacggaac
    19681 gcccgttgtg tgattgggat tgacttgtca gatgtcaatg acatctgctc tatctctttt
    19741 atggttgtgc gtggggaaga acggcactat ctaaacaaga aattcatgcc acggcatacc
    19801 attgagacat tgccaaagga actgcgtgat aagtacacgg agtgggaatt aagtggcatg
    19861 ctgcatgtgc atgaattgga ctacaatgac caagcctata tctttgaaga gttacggcag
    19921 tttatgagtg acaacagaat tttgcctgtg gcagtcggtt atgaccgcta caatgcaagg
    19981 gaacttattc gcttgtttaa cgactactac ggggatattt gtcacgatat tccccagacg
    20041 gtcaaatcgt tatcaaatcc gctcaaggtt tacaaggaga aggctaagat gggcaaaatc
    20101 atctttgatg atcctgtggc gacatggaat catgccaacg tccgtgtcaa aattgatgcc
    20161 aataacaata tttttccaaa caaggaaaag gcaaaagaaa agattgacgt ctttgctagt
    20221 cagctagatg cctttatctg ttatgaaaat ttcaaggaag acttgagcta ctactttgat
    20281 tgaggtgaag aatgaacaac tatttgaaaa atttgaagga ggtttttgct aggattttcc
    20341 gtccaaacaa tcggaaatct acaagaacct atcttcaaag aagtatctcc tactggcgta
    20401 ggaactccat ctatttggat aacatctaca ataagatttc aactgacaca gctcagttaa
    20461 ggtttaagca tgtcaagatt actcgtaacc caggcggtgt cgattcgatg gtttggtatg
    20521 agcatagtga tttggctgag gtgctaacag tttcaccaaa tccactagaa gtgcctgttg
    20581 tcttttggtc aaatgtaact agggccatgc tgcgtgacgg tgtggcggtt gttgtaccac
    20641 gttggaagaa tggccgactg gttgaaatat ggcttgctaa gaagacagtg acttggacag
    20701 cagagagcgt ggagcttatg cttgatgatg ttgctgttga gcttcctctt actgatgtgt
    20761 gggtatttga aaatcctaag ttgaatgtca cggctcaact caatcagatt actgagctta
    20821 ttgacattaa cttgaatgct ttgacagaaa agcttagcga tggcaattct agcttgagag
    20881 gtttcttaaa gctaccaact aaggcagctg atgaacattt gaagcaacaa gccagggacc
    20941 gtgttgatag tatgcttgac ttggccaaga acggtggcat tgcttatctt gagcaaggcg
    21001 aagaattcca ggaacttagc aaagactatt ctactgcttc taaggaagaa ttagagttct
    21061 tgaaatcaca gctttataat gctcatggta tcaatgagaa attatttacc tgtgactata
    21121 ctgaggaaca atatagagcc tactattcta gcgtcatgaa gttatatcag cgtgtctatt
    21181 ctgaggagat taacagaaaa tacttcacga agacagcacg gactcagggc aataagttac
    21241 tggtcttctt tgatatggct gacatgattt catttaagga tttggttgaa ggtggcttca
    21301 agtccaaata tgctggattg atgaactcaa atgagttccg tgaaacctat cttggtttac
    21361 ctggctatga gggcggtgaa gtatttgaaa ccaacctgaa tgctgttcgt atcggtgcag
    21421 aagaatctga gtagaatcta aagggtgggt ggttggcata tctttcacga aaggaggtaa
    21481 gcaatggaaa aactaaaaac ctttgttgtg aagtcggttg aggatgaatc agctgacttt
    21541 cattttgagg cttacgcttc aacctatgac aatacagaca gagaaggcga tgtgatggcc
    21601 aaggggtgtt ttgataacac tctgaaatct aaggctgttg tccctatgtg tcttaatcat
    21661 gaccgtaact gtgttattgg taagcatgaa ttgtctgtgg atgaaaaagg cttgcgaaca
    21721 cgttcaacgt ttaatcttag tgatccagag gctaagaaaa cttatgacct catgaaaatg
    21781 ggggcattgg atagcctgag tattgggttc tttattaaag attatgagcc tattgacgct
    21841 aagcaacctt atggcggatg gattttcaag gaagttgaaa tctttgaaat ttctgttgtg
    21901 accgtgccag ctaaccctca agcaactgtt gacaatatta aggaatttga tatgtcggcg
    21961 gttgataagc gaatcgctca ggtgaacatg aggcaagaaa tcatgagtaa acttgcaaaa
    22021 atttaaagga gacatgatga aaaaatcact cattgagctt ttggaagctc gtcaaaaagc
    22081 aactgatgaa ctggctgagg taaagcttaa aaaagccact attgaagcaa agatgaaatc
    22141 ttcaaccatt gaagacgatg acttggaaca gttaaaaact gatgcagaaa gtttggtttc
    22201 tcaagcaaca gctattaagg aaacaattgc tgggctggat tctgacattg aagaaactga
    22261 agaagaactc agcaaagctg ctaaaatcat taaagaaaaa cagaaaggta atacacctat
    22321 ggattatttg aaaaccaaag ccgctgctct tgactttgtc cgtattctga tggacaacga
    22381 aggtagcgca aacagtgccc gtaaagcgtg ggaagctaac cttgttgaaa aaggagtgac
    22441 taacctcact aagattcttc cagaacctgt tcttatcgct attcaagatg cctttactaa
    22501 ctataatggg atcttgaacc acgtttcaaa agatccacgt tatgcggtgc gtgttgctct
    22561 tcaaactcaa gtatctcaag ctaaaggaca caaagctggt aagactaaga aagatgaaga
    22621 cttcacattc ttggatttca ctatcaactc tgcgacagtt tatatcaaat atgcctttga
    22681 atattctgac ttgaagaaag atacaacagg cgcttacttc aactatgtga tgaaagaact
    22741 tgctcaaggc ttcatccgaa ctattgaacg tgctgttgtc attggtgatg gaaaatctaa
    22801 cagtgctgaa gacaaaatca ctgaaatcaa atcaattgct gaagaaactg aagcgaatct
    22861 ctttgaaaca caagaaatca atgttgctgg tgtatttgac aatgctgttc ttgaaacgct
    22921 ggttgctggt attgataaga tggttccaaa tactactcca attcttgtca cttcaaaagc
    22981 cattgctcgt aaattgaaat tggtcaaaga tgcagaaggt cgctacatcg atcctcaacc
    23041 atttgctcca attgcaacca acggaaatgt tattgctggt tttcaagttt acatctatga
    23101 ctggatggac ggtgcaacta acccaatcat tgcatttgct gaccaagctt ataagatgat
    23161 tggtgatgag gctgccgctg accgctttga tgattatgat gtaacagtca accgccgtca
    23221 tattgagctt gctagtgtta tgggtggtcg tttggctcag tacaaatcag cggttaagtt
    23281 tacaaaccaa gcaggttaaa tatagaaagg ggagtctgaa tgacaatcct taaaacaatc
    23341 aaagaaatgg tagaagttga cgttgaagag gatatctttg acgtccaact tttacgatat
    23401 atcaacagtg ggatttcata tttacaaaga aatgctattc ctgttatccg tatcgataaa
    23461 gaaacggagt tagagggatg gacagacatt aaggaagaag atagggagac agtgcttgac
    23521 tggttacatc tgagatgcgt ccaacgtttt gacaaatctt tgatgacagg cagtgctgct
    23581 actatggaat ggattgacgg agagatgaca aatcttttgt atcaactcaa ggcaatctat
    23641 gaagtgaaga catgaaatct tcaagagtag ctatcattct ttgctatgat gagcgtgttg
    23701 aggttgaaaa aggtgtctgg gagaaaaaga ccgtagaaaa gaaagtcaaa gctaagaaag
    23761 agaaaatcta tcagcgaagg cttgataaag ctatggcaga cggtcaagtt ctgactgcta
    23821 ggtttagtgt tcgttccaac tatgtgactg acactcttga ctatgtcaaa tataatggca
    23881 aagagtacaa ggttaacatt gggacagaat cagatgatgg tcattacact atcatcgaac
    23941 ttggagaact gaaataatgg ccaagaaatt tttcactaga caagatattc aatctatcct
    24001 tgaaacaaac cctttgaagg ctaaggtctt ctacatggaa cgtgaggaga aatcttctcc
    24061 tgacaatgtc atcttgtatt atcggttaac accaggcagt agcattactg ctgatgatag
    24121 agtgcacatg agaaaagtga ctttgcaagt tagtcactac cacaagaaga aactagacag
    24181 cattgaaaat ttgatgctgt ctaattttat gtgtgagcca aatcaattga atctaaaaca
    24241 acctgacact gactatctac tgacaactta cagacttgag gtgtttacaa atgggcggtg
    24301 gtagcgtgaa gatgacaccg ctaaaggtgg atatcaagaa tcaagtttta gagaacatta
    24361 agaaggctgc ccaaagtaca gaaaatgaca taagaaccaa aagccctaga cgaaacggtg
    24421 tttacgaaaa aggttggaca cacgacatca tagatgacgc cgctgttgtt cataataatg
    24481 gcaaagaaaa atctctatcc cacctattag agaatggtca tgccactaaa aacggtggct
    24541 ttgttgcccc tagagagcat atcagaccag cctacatcaa gaataaggag aaattcctta
    24601 atgatatgaa atccattaaa atcacaccta aataagaaag gagtcttaca tgacttatca
    24661 atacgataca agggaagtca ctcacggtaa tgcgaatggt ttttatgcca aaattgctaa
    24721 aactgatgct ggtgccttag acttgcagaa accttatccc tttacagggc tacgaagcac
    24781 atcctttgaa acttctcagg aatcaaatgc ctactatgca gacaatgtgg aacatgttcg
    24841 tttgcaaggt aaaaaatcca cagaagggtc tattacaacc tatcagattc ctaaacagtt
    24901 tatgattgat cacttaggta aaaagcttac gaactctaca ccaccagctt tgattgatac
    24961 tggtgtcaat actaacttca tttggggtta tgctgaaact gttacagatg agtttggagc
    25021 tgagattgaa gagttccaca tttggacgaa cgttaaggca tctgcaccta aaggcagcac
    25081 atcaactgat gaaacatcag cgacaccaaa agaaattgaa atcccttgta ctgcctctcc
    25141 aaataacttc attgtggatt ctgaaaagaa acctgtatca gaaattgtat ggcgtgatga
    25201 cagcaaagga accgtccgtg ggaagtttga caagttgttt gctgacaagt caccaggcaa
    25261 gctgattgat ttcatcaatg aagcgcttgg aacaaccact acacctacca caactgttag
    25321 cggaggctct taatgattaa aaaagaacta tcattcacag cgtttgatag ttacggtgaa
    25381 gaaagagagc acactgaaac agtgcgcttt ctttactctt taccagctat caagatgtat
    25441 gaacagcgaa ctggtcgtaa cttctttgat gataaccaaa aagcacttac agcatataca
    25501 caacttgctc ttgcaactgg tgtaaacggt aacctatctg atttaactga tgaagagaaa
    25561 atcaagatga tgcctctact tatggaacct gatttcatga acttcttgac tgaagtcatt
    25621 ccttgccttt atggtgaggt tgaaaatgga cgtttagtac aaaatgagct taccgctgaa
    25681 acagcttcat tagctccttg gtttggtgat ttgattgata ttggtttctt ctcagacctc
    25741 ttctatgaat ttaaccgaag cagagcaaag gtgccgcaag ataaaaaaaa gccttttcag
    25801 aagttgtaac ttccgaaaag gtttataagg ttatctttga aaatcggatg gatgtttttt
    25861 gggcagaatc ccaacacttt aactatttga tgggaacact tcaccaaatg agcatcaatc
    25921 aggaagagaa gaaaacctta tccaacgctg atttacttag cgtgatgtca gattaaaaag
    25981 aaaggaggaa atctatgact gaaacgtttg aaggcttata cgtcaaattt ggtgctaata
    26041 ctgttgaatt tgataggtct gtaaaaggta tcaacactgc cttatctagt ttgaaaaaag
    26101 acttcaataa catcaacaga caattgaaga tggatccaga caatgttgac ttgttgaatc
    26161 gtaagttggt taacttgcaa gaacaggctc gtgttggtgc tatgaaaatt gctgaactca
    26221 aaaagcaaca gaaggcactg ggagaatctg aagttgggtc agcacagtgg aataagcttc
    26281 aacttgaaat tgctaaggtt gaatcacaga tgaagattgt tgataaggca atggagtcaa
    26341 caaagaaaca cattgaagat gtaggagacc caaagtctat tctgaatctt aacaaagaac
    26401 ttgataatgt tgctaaagag cttgatattg tcaatcaaaa gcttgagcta gacactgaca
    26461 atgtcgaact agcagagcaa aaaatgaaac tacttggtaa acagtcggaa ttggttgggg
    26521 ataaagtcca agaattaaag aaaaaacaag ctgcccttgg cgatgagaaa ataggtacag
    26581 aagaatggcg tcaacttcaa aatgaaatcg gtcaagctga agttgaagtt ctaaagattg
    26641 accgtgcaat ggacattctt ggtgagtcaa gccgttctgc aactggagac atcaaagagg
    26701 caaccagcta tttaagagct gatgtcatga tggatgttgc agataaggct ggtcagattg
    26761 gccagaaaat ggttgacgct gggaaaatga cagtagatgc ttggtctgag atagatgagg
    26821 ctctggacac cgtcacaacc aaaactggtc tgactggtga tgccttagca gagcttcagg
    26881 aaattgctaa agacattgct actggtatgc ctaccagctt tcagaatgct ggtgatgccg
    26941 ttggggaatt gaatactcag ttcggtttga ctggggaaaa gctgaaatca gcatctgaat
    27001 tacttatcaa gtatgctgag attaacgaaa cagacatttc aagctctgcc atttctgcaa
    27061 aacaagctat tgaagcttac ggtttgacag ctgaagactt gggaatggtc ttagacaatg
    27121 tgaccaaagc cgctcaagat acaggacagt cagttgacac gattgttcaa aaagccattg
    27181 acggtgctcc tcagattaaa ggtttgggac tttcttttga agaaggtgct gcactgatcg
    27241 gtaagtttga gaaaagcggt gtggattcat ctgctgctct atcctctcta tcgaaagctg
    27301 ctgtcatcta tgctaaagat ggtaagactc tgacagatgg attgaatgag actgttagtg
    27361 ctattcaaaa ttctactagt gagacagagg ctttaagtat tgcctcagaa atctttggta
    27421 gtaaggctgc tcctagaatg gtcgatgcta ttcagcgtgg tgcttttagc ttcgatgact
    27481 tagctgaagc agctaaaagt tcctctggta ctgtctccac cacatttgat gagacgcttg
    27541 acccaataga taagttgact cagtattcta accaagcaaa agagggaatg gcagaacttg
    27601 gcggtaaatt gcttgagact gtcatcccag ctttagaacc tttgatgggt atgcttgaat
    27661 cttctgtcaa ttggtttact agcctaaacg aaactgatca acagactatc gtgattcttg
    27721 gcctagttac aactgctgtg atgatgttgc ttgatgcaat tgcaccgctg gtcatcgcca
    27781 taggggcaat aggtgcgcct gtcggaattg tagtggcggc aatagtaggg gctattgccg
    27841 tcataacact tatcatccaa gcaatcatga actggggagc cataactgaa tggcttcagt
    27901 caacgtggga ttcttgtgct gcctggcttt ctgaattgtg gactaacata gtcacgactg
    27961 ccaccacagc gtggtcaaat ttcactgcct ggctttctgg cctttggtct tcagtagtct
    28021 caactggaca gtctttgtgg tctagcttta ctagttcctt gtccaatatt ttctcaagtt
    28081 tgattacagg tgctcagtct ctgtggtcaa gtttcacttc cactctttcc aatttgtggt
    28141 ctggactggt ctcaactggg tcaaatttgt ttaataattt gagtagcacg atttcaggaa
    28201 tttttaatgg gatactttca acagcaagca atatttggaa ttccataaaa tccactattt
    28261 ccaatgcaat agatggggcg aaaaatgcag tgtccaacgg ggtcaatgcc atcaagaatc
    28321 tgtttaactt ccagattaaa tggcctcata ttccactacc tcacttccgt gtgagtggtt
    28381 ctgctaaccc tctggattgg ctaaaaggtg gcttaccaag tatcggcatt gactggtatg
    28441 ccaagggcgg tatcatgacc aaaccaaccc tatttggcat gaatggaaac cgtgcaatgg
    28501 ttggcggtga ggctggcgct gaagccatct tgccattgaa taagtcaacc ctgggggcaa
    28561 ttggtcaaag tattgctaac acgatgaata catcgaacaa tattaacgtt aacttctctg
    28621 gcgtcactat cagggaagaa gctgacctta acagactagc caacgtggtt ggaaatcgta
    28681 ttgctgaaga attgcaacgt aaaactaatt tgagaggagg aatggcatga caaaaatcaa
    28741 tgaacttacc attgacggtg tgaaaacatc atcttttaag tgtgatgtat tggttgaaac
    28801 tagaccaaat gtcattgtct ccagctccaa aacagctcta ttagagcatg atggtattag
    28861 tggtgcagtt gtgcaatcga atagacaccg tgggctaatt gagaagcctt atcatatcac
    28921 tttaattgag ccaagcgatg aagaaattta tcgcttttct gctcttttga accgtgaaaa
    28981 gttttggttg gaaaatgaac aggaaccaac tattaggctt tggtgttata aggttgatag
    29041 ctttgagatt ggaaaagacg aatttggtgc ctgggtggtc gatgttacct tcatctgcca
    29101 ccctaccaag ttttttaaaa caactgacac ccagactttg actgggaatg gggttttgag
    29161 ggtgcaaggg tcagctcttg ctttcccgaa gattacagtg gttggtcaaa gcgcttctga
    29221 gacatcgttt acggtgggga atcaagtgat taaacttgaa aagctctcag aatcgcttgt
    29281 gatgaccaac gatcctgaca atcctagctt taagacggct agtggcaagc ttattaagtg
    29341 ggctggtgat tttatcacag ttgatactgc taaggggcag aatgtcggtg ttgttttagg
    29401 tgctggcata acgtccttga aatttgaaac agtttggggg tgggcatagt tgctcttttt
    29461 acttgataaa gatgttagaa cggtcaaatg gaatggcatt ccacttcacg aggccagctc
    29521 tgccattgtc aaagaagaaa ccaacagtga tttctacctg actgttcgct atcctatcac
    29581 ggattcaggt atctatcaac ttatcaaaga ggatatgctg attaaggcgc ctacgcctgt
    29641 gctgggtgct cagctgttta gaatcaagaa acctattgag aatgatgata gcttggatat
    29701 cactgcttat catgtttctg acgacatcat gaagcggtct atcactcctg taagtgtagt
    29761 tggtcaaggt tgtgctatgg cactgtctca gatggttcaa aatgctaaga ctggtctagg
    29821 tgatttttcg ttcaccagcg atattatgga cagcagaacc ttcaacacga cagagacgaa
    29881 aacactgtat tcagtcctta tggacggtaa acacagcatt gttggaacgt gggagggtga
    29941 gcttgtccgt gacaattttg ctctttctat caagcgtagc cgtggggctg atcgtggtgt
    30001 tgtcatcaca acacacaaga acctcaaatc ctatcaacga accaagaact ctcagggtgt
    30061 tgtcacaagg attcatgcac ggtcaacctt caaacctgat ggagctgaag atgaagtgac
    30121 gctcagagtg tctgttgaca gtccactaat aaactcttat ccctacatca atgagaaaga
    30181 gtatgagaac aacaacgctg aaaccgttga agaccttaga aagtgggctg aggctaagtt
    30241 tacaaatgag ggcattgata aggtttctga tgccattgag attgaagcct atgagcttga
    30301 cggtcaagtt gtaaatctgg gcgacacggt taacctcaag agtagaaagc acagtgctga
    30361 cctctacaag aaggccattg cctacgagtt taacgcttta acggaagagt atatctccat
    30421 aacttttgat gataagccag gagttggtgg ttctggggtg tcaagtggct tgtctaatgt
    30481 tgctgatgct atacttgtag caagtgccac agctcaggac gttgctgttc aaagggctgt
    30541 gaaaaatgct aacgctgcct ttgatgcaga gtttggtaaa acaaaaacaa aaattaatga
    30601 tgatatcgaa atcgcaaaag ccaaagtaga aagctttaaa tcggaactat ctaatcgcat
    30661 ggataatcag cttcttcccc ttgcgacaga agctaagaat ttagccagtc aggcacaagc
    30721 agacctgacc agaaaagaaa tagagcttcg agctgaatta aatagacaag tcacaagcac
    30781 agaagctgta aaaatctctc taacaaatct tagtcacaat atggatatca ttaagcaaaa
    30841 agccctaaac gaccttagag acgcagagac aaggctaaag gaagctgata gcgtccaaca
    30901 acttgcgaca aagcgggttg aggataaact gacaggactt agcacgaaac tggagtcatt
    30961 ctctgttggt ggctataatt atgtcattga cggcggagaa cctaaagaac taatggctaa
    31021 cttctatggt aagacctatg atattaatcc ccaactactc gaacgaacta gtcaagcgac
    31081 cttgagtttt agctatgaag cagaatcaac tagtcgttta gaagttagac tttataaaaa
    31141 gatgcacacc ggggacacta gtaagattac aattattgtc atgcctaact ttgacttatc
    31201 tccagggaaa ggatttatct ctcagagctt tgatttaggt ggtgttatgc ctgaccctag
    31261 aaaccaagcc tggcttgtga tgcgggggac aaatgctaac cccttgacgc tctcgaaagt
    31321 taagctggaa cgtggtaccg ttgctacaga ctggaataat cgcgatgaaa ccttaaaagc
    31381 tagcttcgcc gaatacaagc agaccgttga tgaaaactta gccaacctta gaacaagtac
    31441 ggaaaccttg gcaggtcaac tgacaagtgc tgagtctagc attaggcaga cgtcggaatc
    31501 ctttagcaat cgtttggtta gtcttgagac ctataaagat agtgaatcaa atcgagctag
    31561 tcgttacttt gaagcaagca agtctgaaac cgctaagcag ctatcagcct tacgaacaga
    31621 agttaatggc tcttatgtgg ataagtcaac ctatgaggaa aatgcaagag gtttgacaag
    31681 acgctttgaa agtctgtcat cagggacaca gaactatgct ttggggactg aaagagatgt
    31741 ctatctaaac gtttcaggga ctaccgtcta tgacctttat agcttcggca ctaccttagc
    31801 aataaaaggg attgaaatgg gagatactgt aactattgca tttaactatt ttgcggatag
    31861 tcggacggct tttggttctt atgaactgga gctatacgga catgcaggca aaatcgaacg
    31921 ggttggtgag gtacagagaa cgatatcaaa tcgtggtacc tataccgcat cttttgtggc
    31981 aaacaatgcc aatttcaggg caaactccct taaaattaga tttacggata gccgacttaa
    32041 gtttagagtg acaacacttc gtgtgactaa ggggacaatt cctgcggact ggagtccatc
    32101 tccagatgac ctaaaagcct actcggatac taagcttgaa cagacagcta atgagattaa
    32161 agctagtgta acaagtcttg atcataaaac tcttaagcaa actgatatta ctatgacttc
    32221 tgaagggatt gtcctaagag caggcaagac gagtaatgat gtggcaagag ctattggctc
    32281 ttactttaag gtcactcctg atgctatagc tctgtttagc tcactgataa aagttagcgg
    32341 gaacatgttg gttgatggtt ctgtaactag tcgtaatttg gtaacaggcg ctgtcgaaac
    32401 gggacatgtt aaggctggag caatcacagg agtgctttta gcagctgaag ctgtaacggc
    32461 agaaaaactt aaagtcgacc aagctttctt taacaagttg atggccaacg atgcctactt
    32521 gaagcaacta tttgccaagt cagccttcat cactcaggtt caatccgtga caatatctgc
    32581 cagtcagatt tcgggcggtg ttattaaagc tttgaataat gccatggaaa tccagatgaa
    32641 cagtggtcag atactttact acactgacca agctgcactg aaacgggttt tgagtggcta
    32701 tcctactcag tttgttaagt ttgcaactgg tacagtatct ggtaaaggaa atgctggtgt
    32761 tacggtcatt ggttctaacc gctacggtac agaatcaacc aatgatggtg gatttgttgg
    32821 agtccgtgca tggaacggct caaacattga ctcacttgac ttagttggtg ataaaatccg
    32881 attagcaagc tctgcctttg acaattcaga tggttgggat gttcggacac ttgattcagg
    32941 cttgaaaatc acaccacaca atagggctgc tgaacgcaat agccgaattg aggttggtga
    33001 tgtctggatt ttaaaaggaa atggatccta ttcttctctc agagatattt tgaactcatt
    33061 caatgagaac ttctcaaaag gcccaaatgc tgactcttac acttactacc attcggggtt
    33121 ctaactagaa aggaacaatc atgaaccaag aacaacaact caatcaagcg ctacgcttga
    33181 ccgtcaatga attgacagcc cagttggcca atgagtcaac cactaagaac ttgctggcta
    33241 tccagctgac tgaagttgat caggagaaac agcaactgac tcagcaaaat gctgaacttc
    33301 aagcaagagt ttccgaacta gaaggcttgc ttgatgaaca aactcaacca gaaatcattg
    33361 aaggagaata accatgacta caacagaaaa taatttactt gaccttgaaa caatcacaga
    33421 accctttgac ttggcgacag cactcaagta catgaaggaa aatggtgagt taatccgttg
    33481 caagaatgct gtcaatgact tctacatgta ccgtgacatg cagaaacgtc ctgtcatcgt
    33541 caatggtcgc cgtcaattca aggacgtgga aactgtctgg gcatttaacc aatggggcgg
    33601 gacaacaccc acaatcaaca tggctgatct ctttaatctt gaatactaca tcatgacctt
    33661 tgatgagaac ggaaatcctg actggactga gcctcacttg gaggataagt aagctcatct
    33721 aaaataaaca ggataggaga tagtgaatgc caccatggtt aatagatagc actgtggttg
    33781 tggcgatggt gacggtgctg ggcggtctgt tcagcactat tatcaccact tcagccaatc
    33841 ggaaagacca gctgataaaa catcagtatg aggatatcaa ggaagatttg tctggcttga
    33901 ttgacaaggt taagacgatt gatcatacaa ccacggaaac aaagaaaatt tcagaaatca
    33961 caaaagacgg tacgcttaaa atccagcgtt accgtctttt tcacgatttg acaaaggaaa
    34021 tttctcaagg ctacacaact attgaacatt tcagggagtt gagcattctc tttgagagtt
    34081 atcaactgct tggcggaaat ggggaaattg aagccttgtt tgagaaattc aaacagttac
    34141 caattgagga ggactaaaca tgacacaaat tacagatatt atcatcagct cagctatggg
    34201 aattttgact attttggcag gaattgcagt gcaagctgtc aaggtttatt tgattaaaaa
    34261 aggcggtgaa aaggccgtct tgattacaga aatcttggcc aagaacgctg ttaatgctgt
    34321 ggagcaagtg gccactgaaa ctggcttcaa aggagctgac aagctaactt ctgctaaggc
    34381 tcagatttta gctgagttgc aaaaatacaa tatccacatg tcagacgatg atctcacttt
    34441 gtttgttgaa tcatctgtca aacaaatgca cgatgcctgg aaggagtaac ctatggaaat
    34501 caacacagaa acagccattg cctggatgtc tgcacgtcaa ggcaaggtca gctattccat
    34561 ggactaccgt gacggaccta acagctatga ctgttccagc tctgtctatt atgctctcag
    34621 gtcagctggt gcaagctcag caggttgggc ggtcaacact gagtacatgc acgattggct
    34681 gattaaaaac ggttatgagc ttattgctga aaacgtggat tggaatgctg tccgtggtga
    34741 tatagcaatt tggggcatgc gagggcactc aagcggagct ggtggtcatg tagtcatgtt
    34801 tattgaccct gaaaatatca ttcactgtaa ctgggcaaat aatggcatca cagtgaacaa
    34861 ctacaatcag acagcggctg ctagtggctg gatgtattgc tacgtttacc gtttgaaaag
    34921 cggagcttct acccaaggaa aaagccttga taccttggtc aaggaaaccc ttgctggtaa
    34981 ctacggtaat ggcgaagcac gcaaggcagt gcttggcaat caatatgagg ctgttatggc
    35041 agtcatcaat ggcaaaacta cgactaatca aaagactgtt gaccaacttg ctcaagaggt
    35101 aatcgctggc aaacatggca acggtgaagc tcgtaaaaag tcgcttggta gtcaatatga
    35161 tgcagttcag aaacgagtga cggaattgct caaaaaacag ccctctgagc cgtctaaggc
    35221 tcaagaggta aacaagccca cggaaaccaa aacaagccaa acagagctaa ctggacaagc
    35281 cacagccacc aaagaagagg gcgacctctc tttcaatggg actatcttga aaaaagcggt
    35341 gctggacaag attctgggca actgtaaaaa gcatgatatt cttccaagtt atgcactgac
    35401 tatcctacac tatgaaggtc tttggggaac ttcagcagtt gggaaggcag ataataactg
    35461 gggcggtatg acctggactg gtcaaggcaa ccgtccaagc ggtgtcactg tcacacaagg
    35521 ttccgcacgt ccttcaaatg agggcggtca ctatatgcac tatgcaagcg tggatgactt
    35581 tctgacagac tggttttatc ttctaagggc tggtggctct tacaaggtgt ctggtgctaa
    35641 aaccttcagt gaggctatca agggaatgtt taaagttggc ggtgctgtct atgattatgc
    35701 tgcaagcggc tttgatagct atattgtcgg agcttcaagc cgcctcaagg ctattgaggc
    35761 agaaaatggt tcactggaca agtttgataa aactaccgac attggtgacg gtagcaaaga
    35821 caagattgac attactattg aaggtattga agttaccatc aacggtatca cttatgaact
    35881 gactaaaaag ccagtttaga attagatacg ataagacaaa ataagccctc agcaattgcc
    35941 gggggctgtt tttccgttat aacggacttt cctcaaaata tcggttataa tggacttttt
    36001 ttgataaaaa ttttaaaaat atttgttaaa aacaagtgtt ttctattgac aagtgtttaa
    36061 aacaagtgta taatacaatt aaagataagg aaaggagata agccaatgac agagcgagag
    36121 cttaagaaaa ttgctaagaa gcaaggtttt agtaaaacta actttggcaa agggtcacac
    36181 gaagtttgga aacatccaga cggacggaca gtgacgatac ctaagccaaa ggaaaaagat
    36241 tacagaccag gcacactgaa caacattctc aaagtcttgt atggggagtg aggacgctcc
    36301 tccccgtacc cctaaaaggg gatactctga tcattggctt aatctaatat tatgaaatac
    36361 acttatttag cattgtttga agtggataaa gaaaacggtg gctataacat ttctttccct
    36421 gattttcctg gagcatttag tgaagctgat agcttgaacg aggccatttt taacgctcgt
    36481 gaagtccttg aaatctatac aattatgttt gaagacgagg gcaaagagtt tcctaaagca
    36541 tcatcattca aggctcttgc aagcaattta gcaagtgacg aagatgtgat tcaggctatt
    36601 tcagttgata ctgagcttgt ccgtgagcgt gaacgctcta agattgtcaa caagactgtc
    36661 acactgccaa gctggcttgt ggaagttgga aaagaaaaca aggtcaactt tagccagctg
    36721 ttgcaaaaag caatccgtga ggaattgcaa gtataagaaa gagcggtcaa gatggccgct
    36781 tttttctgta taaaaaatta tgaaaataga tagtaatact tttcaaaaat tgacctatga
    36841 aaaaccgtcc gccccaaatt cgccccaaat tatttttaaa gttagcctga tttaacctga
    36901 tggaaaatca aaaaagccta taaaatcagg cttttcattc ggttaattcc tgttaattca
    36961 ggtactgaaa ggcggtagac ggatttgaac cgacgatcaa gcttttgcag agccgtgcct
    37021 taccacttgg ctataccgcc ataacagata atattctatc gaaaaaaaaa agattcgtca
    37081 agtctctttc gtaaaatagc caattttagc ccttaaactt cctcatatta ccaaaaaaag
    37141 agattgatac aatgggtatc aatctctttt agattattgt ctgattaaat aatcaaatgc
    37201 gccaatggca gctgtggcac cagaccccat ggaaataatg atttgcttgt aggcagcatc
    37261 tgtacagtca cctgcagcaa aaataccagg gatatttgtt gagccaaatt tatcaacgac
    37321 aatttcttga cgttcattga gttcaatgcc gctatccttg agccaagaag tacttggtac
    37381 aagaccaatt tgtacaaaaa caccttctaa atcaatgtgt ttttcttcgt ttgtatcacg
    37441 atcggtatag tttaaaccag tgacatgatc ttcaccaacg atatctttag tagcaacatt
    37501 cttaagaata gttaaattgt cagttttagc tgctcgttct tgcagtactt ggtctgcttt
    37561 gagctcaggt aagaattcta ggactgtaac atgtttagtg acaccggcta gatctaaagc
    37621 agcttccata cctgaattac caccacctat aacagcaaca ttttttcctt cgaaaagagg
    37681 gccatcacaa tgtggacagt aagtaacacc tttattgcgg aattcttcct cgccaggaac
    37741 gttgatattt cgccacttag cacccagagc taggatagca gttttagctt gtaaaatagc
    37801 tccgttagcc agtgttacct caactaattc ttttttctca atgccagttg ctaattgact
    37861 cttgataata tcaatatcgt aagatttggt atgctcttca atttgagcca ttaatttagg
    37921 gccttcggtg tacagtgtac caatcatgtt ctcaatacca actgtttcga taacttgtcc
    37981 accaaaagtt tctgctaaaa tacctgtttt taacccttta cgagcggcat aaattgcagc
    38041 actgttacct gcaggaccac caccaataac tagaacatca tagacacctt tgtcagcaaa
    38101 tgcttcagca tcaagaggtc catctagttg ttctaaaagc tgttcaattg tagcacggcc
    38161 agaagtaaat tcttcttggt ccttataaac agtaggaaca gacatgatac cttttgattt
    38221 gacttcgtct tgatacatgc ctccttcaat catagtatga gtgatatttg gattcaaaac
    38281 agccattata ttaaaggctt gaacgacatc tggacaatta tggcaagtta atgaaacata
    38341 agtttctaga ttgatagttt tctcaatccc cttaattcgt ttgataatat cttcatcaac
    38401 ttttggtgcg cgaccagaga cttgcaacaa agctaaaatg aaagatgtga attcatgccc
    38461 cattggcaag cctgaaaaaa taacgcgact ttcttgccct ttttttgcaa tcccaaaact
    38521 aggttgacgt tttaaatgtg ttgattcaag gctgatacga tcagacatag cgacaatttc
    38581 atctagaaaa tcctttactt tttgagaatt atcgttatct cctaaatcag tttgtaatac
    38641 aatatcagat tctagcaaat ctaaatattg cgctaattga gccttgattt ctttatctaa
    38701 gaccatggat tacctcctgg ttattaaatt ttacctacaa ggtctaaact aggagttaaa
    38761 gtttcagctc cttctttcca tttagctgga caaacttcac ctggatgttg gcggatgtat
    38821 tgagcagcac ggactttatc aatgagagta cttgcatcac gtccgatgcc atcagcatta
    38881 atttccatca tttggatgac accgtctgga tcaatgataa atgtaccacg ttgtgctaga
    38941 ccatcttgtc caaggacatc aaaaccttgt gaaataaggt gtgaagggtc accaatcata
    39001 ggatatgtga tagtaccaac gacatctgag tcatcatgcc atgctttatg aacaaaatga
    39061 gtatcagtag agacagaata aacttctaca tcaagagatt taagtgtttc gtattgctct
    39121 tgaaggtcac caagttcagt tgggcaaaca aatgaaaagt ctgctgggta gaaacaaaaa
    39181 actgcccatt ttcctttaac gtcttcattt gtaacagtga tgaattttcc atcgtgataa
    39241 gcttgagctg aaaattcaat aatttctttt ccgactaatg acataataat gtcctccttt
    39301 attttttaat agcctaatta taaacgaaat acttttaaat ctccaataaa aacgtttaca
    39361 tttttctaag aaaagtatga tttttctaat agagtggaag attttatagc ttgtcaaatc
    39421 taactttttt tgataaacta ctatagttgc ttaaagtgac aaatactcat cttagatcaa
    39481 ttgtggtctt gttgccgaaa ggttaggctg caagtcgaag tctgagagag gaaaaaacat
    39541 ttttaaagga gaacttactc atggcagtaa tttcaatgaa acaacttctt gaggctggtg
    39601 ttcactttgg tcaccaaact cgtcgctgga accctaagat ggctaagtac atcttcacag
    39661 aacgtaatgg tatccacgtt atcgaccttc aacaaactgt aaaattagct gaccaagctt
    39721 acgaatttgt tcgtgatgct gcagcaaacg atgcagttat cttgtttgtt ggtactaaga
    39781 aacaagcagc tgaggctgtt gctgaagaag ctaaacgtgc tggtcaatac ttcattaacc
    39841 accgttggtt aggtggaact cttactaact ggggaactat ccaaaaacgt attgctcgtt
    39901 tgaaagaaat caaacgtatg gaagaagaag gaacttttga acttcttcct aagaaagaag
    39961 ttgcattgtt aaacaaacaa cgtgcacgtc ttgaaaaatt cttgggtggt atcgaagata
    40021 tgccacgcat cccagatgta atgtatgttg ttgacccaca taaagagcaa atcgctgtta
    40081 aagaagctaa aaaacttggt attccagttg tagctatggt tgacactaac gctgatccag
    40141 atgatatcga tgttatcatc ccagcaaacg atgacgctat tcgcgccgtt aaattaatta
    40201 cttctaaatt agctgatgca gttattgaag gtcgtcaagg tgaagatgct gacgttgact
    40261 tcgctcaaga agcacaagca gattcaattg aagaaatcgt tgaagtagtt gaaggatcaa
    40321 acaacgacta atatttaatt tattgtaaat cctaaaacgg ggcagatgca aaccgctctg
    40381 tctcgttttt ttaatcatta tttgaaatta agaggagaac gaaaatggca gaaatcacag
    40441 ctaaacttgt aaaagaattg cgtgaaaaat caggtgcagg cgttatggac gctaaaaaag
    40501 cattagtaga aactgatggt gaccttgata aagcgattga attacttcgc gaaaaaggta
    40561 tggctaaagc agctaaaaaa gcagaccgtg ttgctgctga aggtttaaca ggtgtttatg
    40621 ttgatggtaa cgttgcagca gttattgaag ttaacgctga gactgacttt gttgctaaaa
    40681 acgaccaatt tgtaacatta gttaatgaaa ctgctaaagt tattgcagaa ggacgtcctt
    40741 caaacaatga agaagcactt gctttaacaa tgccttcagg tgaaacttta gaacaagcat
    40801 ttgtaactgc aactgctaca attggtgaaa aaatttcatt ccgtcgcttc gctttagttg
    40861 aaaaaactga tgagcaacat tttggtgcat accaacataa tggtggacgt atcggagtaa
    40921 ttactgttgt tgaaggtggc gacgatgcac ttgctaaaca agtatcaatg cacgttgcag
    40981 ctatgaagcc aactgttctt tcatatactg aacttgatgc acaatttgtt catgatgaat
    41041 tagcacagtt gaaccacaaa attgagcaag acaacgagag tcgtgctatg gttaacaaac
    41101 cagctcttcc attccttaaa tatggatcaa aagctcaatt gactgatgaa gttattgccc
    41161 aagcagaaga agacatcaaa gcagaattag ctgcagaagg taaaccagaa aaaatctggg
    41221 ataaaatcgt tccaggtaaa atggatcgtt tcatgcttga caacactaaa gttgaccaag
    41281 agtacacact ccttgctcaa gtttacatca tggatgatag caaaacagtt gaagcttatc
    41341 ttgagtcagt aaatgctaaa gcagttgctt tcgtacgttt tgaagttgga gaaggtattg
    41401 agaaagcttc taacgacttt gaagcagaag ttgcagctac aatggcagca gcacttgaaa
    41461 aataattaac actcgcttta tattataatt tggcagcaaa atgttgtcag taaatatatt
    41521 ttaaaataag acacttggct taagtcaagt gtcttatttt gctatcgaat tggtggattg
    41581 gcaaatatta aaaaggagag acagtcatct caccttaact tttctagaat aggttgaagc
    41641 cgataatgga taatgttccc atgatgcatc cagctagtat aacacctagc ataacagcaa
    41701 taacactatg tttaaattcc caatctagaa ggctagctgc aagtgtgcca gtccatgctc
    41761 ctgttccggg taagggaatg cctacaaaca atagcaaggc aataaacaat cctttttctc
    41821 cagctacttt ttcaagtttt tgaccaccgc tatgtccttt tttaaggcac catgtgaaaa
    41881 acttaccagt ataaggttta tctgctcccc attctagaac tttgcgagca aagaagaaga
    41941 tgataggtac aggtagcatg tttccaacaa caccgatagc tagcgcttcc cacaaaggta
    42001 ttccattagc gattgcaaat ggcacagctc ctcgtagttc cactaaggga atcattgaaa
    42061 tcaaaaatgt aataatgtaa ttcattaaat aaaacctttc tatggtatta ttccatttta
    42121 tcttatcttg ctagtaatga caactaaaat ccgcggtcat tctagtttta ctatccttag
    42181 atagatttca tttccgtatc ttttaaaaat ttgatataat aatagtcagt tatagtcaaa
    42241 gaggaaggag tgaatttgtg gccataaaaa atacttccga taatattgaa gaatatataa
    42301 aatcattatt agaacaatca ggaattgctg aaataaagcg ttccaattta gctgatactt
    42361 ttcaagttgt tcctagtcag attaattatg tcattaagac acggtttact gaaagccgtg
    42421 gttatgtggt tgagagtaaa cgaggtggtg gaggttacat ccgcattgct aaggttcatt
    42481 tttctgacca acaccaatta tttggaaata tgttatcaac gattggcgag cgtatcagtg
    42541 aacaagtctt tgatgatttg attcaattat tattcgatga agagataatt acagaacgtg
    42601 aaggaaattt gattctggcg acatcaggtg atgacgtctt aggagagcaa gcctctgtta
    42661 ttagagctcg tatgctccgt aaattattac aacgattaga tagaaaagga ttccatttat
    42721 gagtcattat tccataaaat tacaagaagt attcagattg gcgcaatttc aagcagcacg
    42781 ctatgaaagt cactatttag aaagctggca tcttctttta gcaatggttt tagttcatga
    42841 ttcagtagct ggtttgacat ttgctgaata tgagtctgaa gttgcaattg aagagtacga
    42901 agcagcaaca attttagctt taggtagagc accgaaagaa gagattacca attatcaatt
    42961 tttagagcaa tcaccagctt tgaaaaagat cttaaagtta gcagaaaata ttagtatcgt
    43021 agtaggagct gaagatgtgg gtactgaaca tgtcttgctt gctatgttgg ttaataaaga
    43081 tttattagcc acacgtatct tagaattagt tggctttaga ggtcaagacg atggtgaatc
    43141 agttcgtatg gttgatttac gtaaggctct agaacgtcat gccggtttta caaaagatga
    43201 tatcaaagca atttatgaat tacgtaatcc taaaaaagca aagtcgggtg cttctttttc
    43261 tgatatgatg aaaccaccaa gtactgctgg tgatttagct gatttcacac gtgatttatc
    43321 acagatggcg gttgatgggg aaattgaacc tgttattggg cgtgataaag aaatttcacg
    43381 tatggtccaa gtcttaagtc gtaaaacaaa gaataatcct gtcttagttg gagatgcggg
    43441 tgtcggtaaa acagctttgg cgtacggtct agcacaacgt attgctaatg gaaatattcc
    43501 ttatgagctt agagatatgc gtgttctaga gttagatatg atgagcgttg ttgctggaac
    43561 tcgttttcgt ggtgattttg aggaacgtat gaatcaaatc attgcagata ttgaagaaga
    43621 cggtcatatt attcttttca ttgatgaact gcatactatt atgggttctg gctctggtat
    43681 tgatagcaca cttgatgctg ctaatatttt aaaaccagct ttggctcgag gcactttacg
    43741 cactattgga gcaacaactc aagaggaata tcaaaagcat attgaaaaag atgctgcttt
    43801 atctcgtcgc tttgcaaaag ttttagtaga agaaccaaat cttgaagacg catatgaaat
    43861 tctacttgga ttgaagccag cgtatgaagc ctttcataat gttaccattt cagatgaagc
    43921 agttatgact gctgtaaaag tggctcatcg ttatttgaca agtaagaatt taccagactc
    43981 agctatagac ttacttgatg aagcttctgc cactgtgcaa atgatgatta agaaaaatgc
    44041 tccatcgctt ttaacagaag ttgaccaagc tattttagat gatgatatga aatctgcttc
    44101 taaagctcta aaagcaagtc ataaggataa aaagcataag cctattgccg taaccgaaga
    44161 tcatattatg gcaactctca gccgtctatc tggtattcct gttgaaaaat tgacacaagc
    44221 tgatagtaaa aaatacctca atttagaaaa agaattgcat aaacgtgtta tcggtcaaga
    44281 tgatgcagtt actgccatta gtcgtgctat ccgtcgcaat caatctggca ttcgtactgg
    44341 taagcgtcct attggttcct ttatgttctt gggaccaact ggtgttggta agacagagtt
    44401 agcaaaagcc ttggcagaag tgttgtttga tgatgaatca gccctaattc gttttgatat
    44461 gtcggaatat atggaaaaat tcgcagcttc tcgcctgaat ggggcacctc caggttatgt
    44521 tggttatgat gaaggaggag agctgactga aaaagtacgc aataaaccat attcagtttt
    44581 actttttgat gaagttgaaa aagctcatcc agatattttt aatgtattat tacaagtgct
    44641 tgatgatggc gttttaacag atagtcgagg acgaaaagtt gacttttcaa atactataat
    44701 cattatgact tctaatttag gagcaactgc attacgtgat gataaaacag ttggttttgg
    44761 agctaaggat atcagccatg attatactgc tatgcaaaaa cgtattatgg aggagcttaa
    44821 aaaagcttat cgaccagagt ttatcaatcg tattgatgag aaagttgtat tccatagtct
    44881 cagtcaagat aatatgcgtg aagttgtgaa aattatggtt aaacctttaa ttttagcgct
    44941 taaagataaa ggtatggatc tgaaattcca gccttcagcc ttgaaacact tggcagagga
    45001 tggctatgat atagaaatgg gtgctcgtcc actgcgtaga actattcaaa cgcaagtaga
    45061 ggatcattta tcagaactac tattggcaaa tcaagtgaaa gaggggcaag taattaagat
    45121 tggcgtatcc aaaggcaaat tgaaatttga tattgcaaaa tcataaaaca ttccagtacc
    45181 gatgggtact ggaattttaa tataaaaaga aaatgtccaa aatattttgg acattttctt
    45241 atagatttat gaaaagtaaa aggatctata tctaacaatt gcacttgtag atataggtac
    45301 gactgaaagt cgttgaaaaa ttttaggata aatatattat aatatcctaa tcttttgcta
    45361 acttaaaata aatctaaaga ttaccttaag atttcaatcg cttttgtaac catacaatgt
    45421 catgccattg ttcaaattta aacccgattt ttgggaaatg tgcgacttga ttaaatcctc
    45481 tttttttgtg gaatgcaatg cttatatcat ttggtaaact gatacaagcg agtagattaa
    45541 catacccttg gttaactaat tctttctcca atgtatcata tagttggcta ccaatttttt
    45601 tgctacgtac atctttatca aggtaaatag aaagctcgca agtccagtca taggaagctc
    45661 tagcgtagta agttgaagca tatgcataac ctaatatttt cccattttct tcagcaacta
    45721 aatatgggaa agatttgctg atatttttga tacgattagt gaattccgat atactaggaa
    45781 tatcgtattc aaacgttatt gtagtgtcag taacgtaggg agcatagata gcaagtaatt
    45841 ctttagcatc tgagggtagg acaggtctta taatcataga tatcacctaa ttatagtaaa
    45901 tcaagttttt gcaattcgga atcaatagtg tcaaggacgt gttgaagatc ctctggattt
    45961 ttgacgaaat caagtttgtt accatcgatc cgaattttag gtgaaacgtc gtaattttca
    46021 taccattctg gatattcaga gtggacttgt ttgtagtagt cgtataattc tgggttagaa
    46081 tctacttgct caaagctgcg tcctcgtttg tcaatacgtt ctaacatttt atcaaatgaa
    46141 acatcaatgt agactagcaa atcaggtctc ttcttaggca taccttctaa ttcttccaac
    46201 atatttgcaa gaagttcttt atagatatct agctctgttt tagtcacatt tccattctta
    46261 aaatttagag tgaggaatag ttcatcttca aaaatagaac gatccaaaac attgttgtta
    46321 gccttatagg cttcttttat agattggaat cgtttattta a
//
LOCUS       DATRSG010000009        41139 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRSG010000009 DATRSG010000000
VERSION     DATRSG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41139
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.009"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            26..2092
                     /locus_tag="VBD89_000495"
     CDS             26..2092
                     /locus_tag="VBD89_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7135050.1"
                     /translation="MDDIFNSLMGNMGGYNSENKRYLINGREVTPEEFSQYRQTGKLP
                     GQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNKEIQETAEILARRTK
                     NNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDISSLEAGTQYRGSFEE
                     NIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPALSRGELTVIGA
                     TTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLYEQHHNVVLPDSVLK
                     AAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEKEIAEQRDKQEKAVN
                     TEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERLTGVPVSNMGASDIE
                     RLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSFLFVGPTGVGKTELA
                     KQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDNSNTLTERIRRNPYS
                     IVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIATSNAGFGNEAFTGD
                     SDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLMLDEVNQTIGKKGID
                     LVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDHTDVKHLKANLQDGQ
                     IVISER"
     gene            complement(2185..3129)
                     /gene="mmuM"
                     /locus_tag="VBD89_000496"
     CDS             complement(2185..3129)
                     /gene="mmuM"
                     /locus_tag="VBD89_000496"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO7135051.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(3134..4510)
                     /locus_tag="VBD89_000497"
     CDS             complement(3134..4510)
                     /locus_tag="VBD89_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7135052.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILCLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            complement(4640..5290)
                     /locus_tag="VBD89_000498"
     CDS             complement(4640..5290)
                     /locus_tag="VBD89_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO7135053.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            complement(5302..5951)
                     /locus_tag="VBD89_000499"
                     /pseudo
     CDS             complement(5302..5951)
                     /locus_tag="VBD89_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            6043..6591
                     /locus_tag="VBD89_000500"
     CDS             6043..6591
                     /locus_tag="VBD89_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7135054.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKN"
     gene            complement(6653..7249)
                     /locus_tag="VBD89_000501"
     CDS             complement(6653..7249)
                     /locus_tag="VBD89_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO7135055.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(7260..8486)
                     /gene="clpX"
                     /locus_tag="VBD89_000502"
     CDS             complement(7260..8486)
                     /gene="clpX"
                     /locus_tag="VBD89_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO7135056.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(8704..9198)
                     /locus_tag="VBD89_000503"
     CDS             complement(8704..9198)
                     /locus_tag="VBD89_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7135057.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(9278..10117)
                     /locus_tag="VBD89_000504"
     CDS             complement(9278..10117)
                     /locus_tag="VBD89_000504"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO7135058.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            10166..11443
                     /locus_tag="VBD89_000505"
     CDS             10166..11443
                     /locus_tag="VBD89_000505"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO7135059.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            11445..12728
                     /locus_tag="VBD89_000506"
     CDS             11445..12728
                     /locus_tag="VBD89_000506"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO7135060.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            12820..13269
                     /locus_tag="VBD89_000507"
     CDS             12820..13269
                     /locus_tag="VBD89_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO7135061.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQIFAIQEISQTLELLTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            13262..13918
                     /locus_tag="VBD89_000508"
     CDS             13262..13918
                     /locus_tag="VBD89_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO7135062.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            14005..14919
                     /locus_tag="VBD89_000509"
     CDS             14005..14919
                     /locus_tag="VBD89_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7135063.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            15020..15874
                     /locus_tag="VBD89_000510"
     CDS             15020..15874
                     /locus_tag="VBD89_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO7135064.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(15919..16137)
                     /locus_tag="VBD89_000511"
     CDS             complement(15919..16137)
                     /locus_tag="VBD89_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO7135065.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(16205..17200)
                     /locus_tag="VBD89_000512"
     CDS             complement(16205..17200)
                     /locus_tag="VBD89_000512"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO7135066.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(17197..18189)
                     /locus_tag="VBD89_000513"
     CDS             complement(17197..18189)
                     /locus_tag="VBD89_000513"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO7135067.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(18182..19126)
                     /gene="mvaD"
                     /locus_tag="VBD89_000514"
     CDS             complement(18182..19126)
                     /gene="mvaD"
                     /locus_tag="VBD89_000514"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO7135068.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(19108..19986)
                     /gene="mvk"
                     /locus_tag="VBD89_000515"
     CDS             complement(19108..19986)
                     /gene="mvk"
                     /locus_tag="VBD89_000515"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO7135069.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(20102..21331)
                     /locus_tag="VBD89_000516"
     CDS             complement(20102..21331)
                     /locus_tag="VBD89_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7135070.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(21331..22017)
                     /locus_tag="VBD89_000517"
     CDS             complement(21331..22017)
                     /locus_tag="VBD89_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7135071.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYIFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(22019..22645)
                     /locus_tag="VBD89_000518"
     CDS             complement(22019..22645)
                     /locus_tag="VBD89_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7135072.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            complement(22753..24507)
                     /locus_tag="VBD89_000519"
     CDS             complement(22753..24507)
                     /locus_tag="VBD89_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135073.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(24497..26314)
                     /locus_tag="VBD89_000520"
     CDS             complement(24497..26314)
                     /locus_tag="VBD89_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135074.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIGGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(26361..26801)
                     /locus_tag="VBD89_000521"
     CDS             complement(26361..26801)
                     /locus_tag="VBD89_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7135075.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            27070..29142
                     /locus_tag="VBD89_000522"
     CDS             27070..29142
                     /locus_tag="VBD89_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO7135076.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSIPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(29179..29589)
                     /locus_tag="VBD89_000523"
     CDS             complement(29179..29589)
                     /locus_tag="VBD89_000523"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7135077.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(29659..31008)
                     /gene="gdhA"
                     /locus_tag="VBD89_000524"
     CDS             complement(29659..31008)
                     /gene="gdhA"
                     /locus_tag="VBD89_000524"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO7135078.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            31176..31685
                     /locus_tag="VBD89_000525"
     CDS             31176..31685
                     /locus_tag="VBD89_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO7135079.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(31726..33495)
                     /locus_tag="VBD89_000526"
     CDS             complement(31726..33495)
                     /locus_tag="VBD89_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135080.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(33500..35239)
                     /locus_tag="VBD89_000527"
     CDS             complement(33500..35239)
                     /locus_tag="VBD89_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135081.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(35306..35779)
                     /locus_tag="VBD89_000528"
                     /pseudo
     CDS             complement(35306..35779)
                     /locus_tag="VBD89_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(35776..37644)
                     /locus_tag="VBD89_000529"
     CDS             complement(35776..37644)
                     /locus_tag="VBD89_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7135082.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVSELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDMMILENTITRIEDDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSGLDT"
     gene            complement(37655..38863)
                     /locus_tag="VBD89_000530"
     CDS             complement(37655..38863)
                     /locus_tag="VBD89_000530"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO7135083.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHAPLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(39048..39896)
                     /locus_tag="VBD89_000531"
     CDS             complement(39048..39896)
                     /locus_tag="VBD89_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7135084.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(39896..40276)
                     /locus_tag="VBD89_000532"
     CDS             complement(39896..40276)
                     /locus_tag="VBD89_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO7135085.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQIAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(40348..40881)
                     /locus_tag="VBD89_000533"
     CDS             complement(40348..40881)
                     /locus_tag="VBD89_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135086.1"
                     /translation="MTMKTIEEIYKNYLNIPYISPERDLAEINFSKVVPRKNMEETSE
                     GLLPGDIILLWRIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSL
                     DHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLA
                     ALSNNQEVIDRHPKKKF"
     gene            complement(40932..>41139)
                     /locus_tag="VBD89_000534"
     CDS             complement(40932..>41139)
                     /locus_tag="VBD89_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO7135087.1"
                     /translation="VALILLHKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLC
                     VFDVIMIILTWLEYQKMKLNFSKR"
BASE COUNT        13387 a         8195 c         6637 g        12920 t
ORIGIN      
        1 ttataatcgt gacccttttg gaaatatgga tgatattttt aattctctaa tgggaaatat
       61 ggggggctat aacagtgaga ataagcgata cttaatcaat ggacgggaag ttactcctga
      121 agaattcagt caatatcgtc aaactggaaa actccccggt caagagctaa ataaccaaaa
      181 cactcctaca aaccaagtct ctgcagatag tgttttgact aaattaggaa ctaatttaac
      241 cgatcaagca cgtcagcatt tgctagatcc agttattgga cgaaacaaag aaattcagga
      301 aactgcagaa attctagctc gtcgtactaa aaataatcct gttctggttg gtgatgcagg
      361 tgttggtaaa acagctgtta ttgaaggttt ggcacaagca attataaatg gtgatgtccc
      421 tgctgctatc aaaaataaag aaatcatttc gatagatatt tctagccttg aagctggcac
      481 ccaatatagg ggtagttttg aagaaaatat tcaaaatatc ataaaagaag ttaaggaaac
      541 aggaaatatt attctcttct ttgatgaaat acaccaaata ttaggagccg gttcaactgg
      601 tggtgatagt ggctctaagg ggctcgcaga tatacttaag cctgcacttt cacgtggtga
      661 gttaacggtt ataggtgcaa ctactcaaga tgaatatcgt aacaccattc tcaagaatgc
      721 tgccttagca cgtcgcttta acgaagttaa agtcaatgca ccttctgcac aagatacttt
      781 caatatcctt atggggataa gaaacctcta tgagcaacat cataatgttg ttttacctga
      841 ctctgttttg aaagcagctg tagatttatc tattcaatac atcccacaaa gaagtctccc
      901 tgataaagca attgacctta ttgatatgac tgctgcacac ctagctgcgc aacatcccgt
      961 aactgacctt aaatccttag aaaaagaaat cgctgagcaa agagacaagc aagaaaaagc
     1021 tgtcaatact gaagattttg aagaagctct taaggtcaag acacgcattg aagagttaca
     1081 aaaccagatt gataatcata ccgaaggaca aaaagtcact gcaactatca acgatatcgc
     1141 tatgtcaatt gagcgtctaa ctggagttcc tgtatcaaat atgggtgcta gtgatattga
     1201 acgcttaaaa gaacttggca atcgtctaaa aggtaaagtc attggtcaaa atgatgctgt
     1261 tgaggcagtt gcgagagcta ttcgccgaaa tcgtgctggt ttcgatgatg gtaatcgtcc
     1321 gattggtagc tttctatttg ttggaccaac tggagttggt aaaaccgagc tagcaaagca
     1381 attagctttt gatatgttcg gatcaaagga tgctatcgtt agacttgata tgtctgaata
     1441 taatgatcgt acagcagttt ctaagctaat tggagcgact gctggttacg ttggttatga
     1501 cgacaatagt aatacactaa cagaacgtat ccgtcgaaat ccttattcta ttgttcttct
     1561 tgatgaaatc gaaaaagctg acccgcaagt tatcacgctt ctccttcaag ttctagatga
     1621 tggccgttta actgatggtc aaggaaacac tattaacttt aaaaatactg ttatcattgc
     1681 aacctcaaat gcaggttttg gaaacgaagc atttacaggt gacagcgata aagacttgaa
     1741 aattatggaa cgaatttctc catatttccg tccagaattt ctaaatcgtt tcaatggtgt
     1801 tattgaattc tctcacctaa gcaaagatga cttaagcgaa attgtagatt tgatgcttga
     1861 tgaagttaac caaacaattg gcaaaaaagg aattgacctt gtggtagatg aaaatgttaa
     1921 atcacactta attgaactgg gttatgacga agcaatggga gtacgtccat tgcgccgtgt
     1981 catcgagcaa gaaattcgag atcgcatcac agactactat ctcgatcata cagacgttaa
     2041 acacctaaaa gctaatttgc aagatggcca aatcgtcatt tctgaaagat aaaaacaaaa
     2101 cccgtttgga agaatccaag cgggttttct agtggattga aaaaggaaat caaacttacc
     2161 ctaaccttct tcaagatact ttacttattt taaatgtgca gagaggtcag caatatcagc
     2221 tggtctagtt cggcaacaac ctccgacaac ttgggcgcca agtttttgcc agtcacttgt
     2281 attttcaagt agggtgtgtg aatgatcagg gctagattgc caagattggc ttgccccatc
     2341 ataaacctct ccagagtttg gataggtcac aagaggcttg ttagtctgct ctgctattgc
     2401 ttgcaagaaa tcagcaacta acgatggact tgaacagtta atgcccagcg ccacgacctg
     2461 tgggctaaca tcaatagctt tcgccaagtc tgcaacagca ctaccatctg agatggtctt
     2521 accatcttga gaggtgaatg acatataagc ctcaacctga gggaaatcct cggcaagtag
     2581 ctcaataaga gcctcagcct cctgagcgtt tggaatggtt tcaagtgcca gaatgtcaac
     2641 accctcatct aaaagaagct caatacgatg gcgatggaag tttttaagac cttgcttatc
     2701 aatatcgtag agtccagtgt actcagagcc atccgctaaa aaggcagcgt agggaccaac
     2761 atccccagaa atcaaaggat agattctctc tgatttttct tcttttgtca aacttttcca
     2821 aacttgctca cgcgcagctt ttgccaactg aacagtcaag cgaatcaaat cttctgcctg
     2881 actttcagag acaccaactt gagctagtcc ttgtaaagtc gcctgataag ttgatgtcgt
     2941 cacaatatca gcgccagcac gaatataatc ttcatgaatg gtctgaatag ctgctggatc
     3001 ctcaatcaga tatttggctg accaaagttt cccagaaaca tcacaacctc tactctcaag
     3061 ctcggtcccc aaggcaccat gtaggatgag agttttttta ctctctaaaa gttctttaaa
     3121 tcttcccata agatcaccat ttcttgtaac gtagtttgtg ataaatatag cagaaaataa
     3181 taaatggaac accgaagtaa agcccagcac gttgtgagct atcccaagca atccctacaa
     3241 tcgaaatcaa gagcaaaatg attgtgatat aagggagaac tggtgtaaaa ggtgttttgt
     3301 aagacaaatc ttctagttga ttctctttca agaattcctt gcggaaattg atttgtgcga
     3361 ctggaatggc tagccatacg acaacaacag caaagcccgc gattgaaacc aaggctagat
     3421 aaactgtgtc tgcagcgtaa atacttgaaa agagcgacag cactgctcct gccattgaca
     3481 agagaagagc acgcattggg acaccgtgtt tattgatttt cacaacagat tttgacaaca
     3541 taccttcatt ggcaagggac cagagcatac ggcttgatgc gtagagacct gagttaccag
     3601 cagacaggat ggctgtcaag ataacgaagt tcatgatatc cgccgtaaaa gggattccca
     3661 tcttgtcaaa gacatcaacg aatggtgctg tggatacgcc tgcctctttc attggcaata
     3721 gcgaagctag gacaacaatt gtcagtacaa agaaaacaac caagcgaccg attgtcgttt
     3781 taatagccct tggtacagct tctttgggat tatccgtttc accagccgca ataccaatta
     3841 actcagtacc agagaaggca tagttaacag ccaacatgac tgagacaact gcaacgatac
     3901 catttggaaa ggcaccattg gcagtcagat gagtgaagag aatagccttg tgctgacctt
     3961 caaaggaaac tagaccaaac atagcaccta agcacaagat aataaagata atgatagcaa
     4021 taaccttaat acttgagaag aaagactctg cttctgcaaa aaagcgtacg ctaagagcat
     4081 ttaaaccaaa aatcacaagg gcaaaaaagg aagcaaatgc ccaagccggc acatttggga
     4141 accagcgctg catcagcatg gcagcaccta aaaattcagt ccccaaggcg accgtccaac
     4201 aaatccaata tagccaagca acagtaaaac ctgttccagg actgataaac ttagtggcat
     4261 aagtgtggaa tgaccccgtc accggcatgg caaccgccaa ttccccaagt gatagcatga
     4321 ccaaataaac cacaacggca ccaatcaaat aagacagcac agctccaagc ggaccagcct
     4381 gtgcaatggt ataacctgaa ctcaagaaaa gcccagtccc gataacacca cctagcgata
     4441 acataaagag atgacgactg gtcatcttac gctggaattt accttcatct tcataacgat
     4501 gattttccat aacaacctcc aagaaactaa tacttttatc ctaccacaat ctagtcgtat
     4561 tggggaatag gtattagcta tcatatatga taaagaaaaa ctattacaaa aaatcagcga
     4621 atcataaata gcggtaacct tattttttta tccttacaaa attaacgacc ttatgaaaac
     4681 ggtccatttg ccaaggacta ataatataat tggtctcctt atccttcaat agctgtacag
     4741 gtgcaataat gctagttagt acacagacaa tcaacatgat aaaaccacct agccaatctc
     4801 cccaagtgag cattccttct gcactagctt gatagatgta tgcgcaagct attggtacta
     4861 ggagaaatac tgtcgttgct agaccaggat tataccaagt tttaagttta atattcatct
     4921 gaaaaccatg acccaagagc tgcgttagac taaagaacat gactcctaat ccaagccaat
     4981 aagcttgagg aaaagcaata ctagcaatgt aaagcaacca agcaatagta ttaaccaaca
     5041 taatagactg agtattgcct ggaaaacaat ccatcagctc ttcttcatca taaacaacat
     5101 agttaatgat aggtgatgcc ccaccgggaa actgatattc ttcaaattga tgcgctagaa
     5161 gtgccataaa gtaaaaatac aatagatagt gaagcattga ccagtcaggt ctaaaaacaa
     5221 gcaatattac tgctaaaata ataaataaaa ttgctccaaa acgataccag taatttctaa
     5281 aatatgacat gataatacct cctatttaaa ctgtgtttca cgaaaatgtg ttaaaaatgg
     5341 ttttgagcgt gaaagatcaa catcactaaa tgcgtaattt gaatgtcgac ctaaacgctt
     5401 atagattggg gaacgaaagg ctataaagta gttaagaaca atccatatca gcctaaaaac
     5461 caatcaagtc cagaatacaa cttccaatca tgagctaaat aatagacaga aacagggaca
     5521 agacctacta aggtcgtaag aagtcctgga ttataccact tcttcaagga aagattaaaa
     5581 aagaaaagat gcatggcaag ttctgcaaat gcaaaaatca tgactgcaag tgttaaaaat
     5641 ggtatattgg gacaaaaaat cggcaaaaga taaacaatca ctgcaaacca ctgattcccg
     5701 aaaaaggctg atagatggtt taagtcccac ttactagtat cagtttcaac cgatttaagt
     5761 tcaactagca tagtgataag cggaaaacct ccagggaaac caaattcttc gaaaaagtgt
     5821 aattgaataa aaagtcccga aataagtagt gactgcactc gtaaatctca atttccaaaa
     5881 aataatgcta aggcaaaaag agaagcaaga tagggacaaa agaggtacca ttttgttatc
     5941 aatgctttca taagatgacc tccaagattt gtgacatttg ataccatata gtataatcaa
     6001 gtgaacctat aatatcaata cctttggagg ttttatgcac aaatgactga aagtgtgaca
     6061 gttaataaaa agagaataaa acaaatcact caaacacaag aggatatctt aacagctttt
     6121 ggccaacttt tggaagagta tccctatgag aaaattcaga ttagccaaat tgccaagaaa
     6181 tctggctatg ctagaaggac cttttaccga cactttgaca gtcgagatga cctacttacc
     6241 cttttcatcg aaagactaac attaaatctc tttaagcaac ttggtcaatt ggagcaacct
     6301 acttttagcc aagttttcca aaattttttt agttattggt ctgattataa atcccttctt
     6361 ctcattctta gaaagaatga cctactccct caatttcaac aatcctggtt ccgacatatt
     6421 gatttgatag agttaggtcg tggtgattta tcttccaata cctatgctca gcgatttgcg
     6481 attggtggta ttttcagtgt tctaattgag tggattcatc aggactgcca aacaagtatc
     6541 gaagaattaa ctcaagtttc cttcgatatt atcaatcatt taaaaaatta aaaagagctt
     6601 tgcagtagtc tcaacagcta ctgcaaagct ctttttgtca taaactctca aattataact
     6661 cagataaaat agtatcccat gattcatcaa gacctgtgcg gtcaacagat gaaaagacga
     6721 tgaaatggtc ctttttatca aagtttaatt tcttcttaat agacgactca tgtttattcc
     6781 acttgccacg aggtatttta tctgcctttg tcgctacaat aattactgga atctcatagt
     6841 acttaagaaa ttcatacatt tgaatatcgt ccgcacttgg atcatgacga aaatcaacta
     6901 aactaacaac aacacgaagg ttatcacgtg ttacaaggta ctcttcaatc atcttgcccc
     6961 acttagcacg ctcagtttta gacaccttag cgtagccata tccagggaca tcgacaaaac
     7021 gcagtttgtc atcaatgtta taaaagttga gcagttgtgt cttacctggc ttactcgacg
     7081 tacgcgctag gtttttacga ccaagaagcg tgttgataaa gcttgattta ccaacatttg
     7141 aacgaccagc gagagcaact tctgggagat catcctgagg ataatgtgac ttgttagctg
     7201 cactcaataa aatagacgca ttatgagtat ttaaaaattc ttctgccatc tctatctccc
     7261 taagccgtct ctaaaacagg cttgtcagta ccctcaacag cagcctttgt gatacgaact
     7321 tttgttacat cttcttggct tggaatttca aacatgatat caagcatcgt ttcttcaata
     7381 atagaacgta aaccacgtgc accagtcttg cgctcgatag ccttatcagc gatagccaat
     7441 agagcatcct ggtcaaattc caattctaca ccatcataag ataataaggt ttggtattgt
     7501 ttaaccaaag cattgcgtgg ttctgtcaga atacgaacca gatcttctgc agtaagaagt
     7561 tctaacgctg caactactgg taaacggcca ataaactctg gaatcagtcc aaacttttga
     7621 atatcctcag aaattatctc ttgcatataa gaagcgttgt catcaatttt acggcttgtc
     7681 tgtccaaaac caataacttt ttcgcctaaa cgttgcttca caaggtcttc aataccatca
     7741 aaagcaccac cgacaataaa aaggatgttc ttggtattaa tttgaatcat ttcttggtta
     7801 ggatgtttac gcccaccctg tgggggaaca cttgctaccg taccctcaat aattttaaga
     7861 agggcttgct gtacaccttc accagacaca tcacgtgtga tagaaacatt ttcgcctttc
     7921 ttagcaattt tatctatttc atcaacgtag ataataccac gctcagcacg ttcgacatta
     7981 taatcagcag cttgaatcaa tttaagaaga atattctcaa catcttctcc aacgtatcct
     8041 gcttcggtca atgaagtcgc atctgcaata gcaaacggta cattaaggct tttagccagt
     8101 gtttgtgcta agaaggtttt tcctgagcca gttggaccaa tcatcaaaat gttggatttt
     8161 tgcaaatcta catcatcgtc actactctcg gtataactaa cacgcttgta atgattgtag
     8221 acagcaactg ctaaagcacg tttagcacga tcttgcccta caacatattg atttaatatt
     8281 tctaatagtt ccttaggttt tggtacttct gctaaatcag ccagtacttc ctcagctaat
     8341 tcttccttaa taatttcttg tgataaggcc acacattcat tacaaatgaa aacaccatta
     8401 cctgcaataa ttttttttac ttcatcttgg cttttgccac aaaatgaaca atagacattc
     8461 atatcgttat tacgattccc agccattttt tcctcaactt ttctattcat ttaatttaaa
     8521 ataatgttcc ataaaaggca tggattgtgt tgacaaggtt agtgaaagca ttcatcaaaa
     8581 aaactgttcc taatccatat ctttttttct ttatagcagg aattgcacat aaaacacaaa
     8641 tcacagctaa aaatgcggta aagaccccaa aaaggctacg ttgcattgaa tagtattcct
     8701 tccttatttt tcaaaatatg tcacagtaaa agcatgagca ttctgctcat ccttttcaaa
     8761 ataatctcta gagataactt taaactcaga tagattaaca tcaggaaaat aggtatcacc
     8821 tttgaatttt ccatgaactt tagtttttat gattgcttca caataaggca gaaatgcttt
     8881 ataaatactt gcaccaccta caataaataa ggtcttatta tgttcctgaa accattttat
     8941 aacttgttca acactattta ggactgtcac tccatctgct tggaattgtt catcttttgt
     9001 taagatgatt gtctctctac caggtaaaac acgacggttc attccatcaa aggtctttcg
     9061 tcccataagc aaagcctgcc ccatggtcgt ttctttgaag tgatgtaact ctttaggaag
     9121 cctccatggt aaaccgccat taactccaat caaatggtct tcatcttcag cccaaatggc
     9181 aattatttgc tttgtcatga cttttttcct cactaactta tctctagata ttctaacaaa
     9241 ttttacctca aattgcttgt taaaaaacat gagatagtta gattgctaaa tcaaaacgta
     9301 attgaggttt aattgggtca taatcaacta acgcaaaatc atctggttta atatcaaaaa
     9361 agtctgttcc atctggaaca ttcaatacta gtttggggtt acactcagac ggttggcgtt
     9421 ttaagagttc ctgagcttgt tcaaactggt tatcataaat atgaagatta ttgataaaat
     9481 agaaaaactt cccaactctc caaccaaaat gctttgcaat catcatctga agtgctacat
     9541 actgcatagc attaatatga tgtgcaacta acatgtcatt agaacgttgt gttaatgtgg
     9601 catccaaata aagctcacca ttaacgcgtc tcacatcaaa catcgtttga aaagcacaag
     9661 gtagtaggcc ttctgtttct tcaaatgctt catagtccca taatgaaata acattacgac
     9721 gattccaagg gttatcttca agctgtttta agagttttga tataatatta tgttttttaa
     9781 caactgcacc gtagcgctga cctatcgtgc cagtatggcc aacttcccaa tcattccagt
     9841 atgtcacacc atatttatca ttcaaaactg ctaaatcatt tgtctgatct tgataaatcc
     9901 agaaaatttc tttaatagca gattttattg gaattggacg tagagttgta ataggaaatt
     9961 caccctttga taaatcatac tctgcaaagg cacctgtaat atacttagaa ttagccattt
    10021 ccccattttt atatctaggt ctggcttgct cgctaaatac accttcagac ataattttcg
    10081 tgatattgtc tttaaataat aaatcggctt ttgtcatgag atacctccaa agtcttattt
    10141 tatcagattt taaaaagata ttctagtgag aaacaagact ttgaaggtta aagctttttt
    10201 tcatattttt agcttcttta tggtaaaatg gaatttagca aaaatgactt ttattcagaa
    10261 aggtttacca atgaaaatag gaattgataa gattggtttt gcaaccagtc aatatgtcct
    10321 tgaaatgact gatttggcta ttgctcgcca agttgaccct gaaaaattca gcaagggatt
    10381 attgttagat tcacttagta ttacgccagt tacagaagat atcgttacac ttgcagcttc
    10441 tgcagctaat gatattttat ctgatgagga caaagaaact attgatatgg tgattgtcgc
    10501 tacagaatca agtatcgatc agagtaaggc ggcttctgtc tatgtacatc aacttttaga
    10561 gattcaacct ttcgcaaggt cattcgagat gaaagaagca tgctatagcg caactgctgc
    10621 tcttgattac gctaaattac atgtagaaaa acatccagat tctaaagtat tagtgatagc
    10681 ttcagatatt gctaaatatg gcattaaatc tactggagaa tccactcaag gtgcaggcag
    10741 tatcgctatg cttattagtc aaaatccatc cattctagag ctaaaagagg accatctagc
    10801 gcaaacccgt gatattatgg acttttggcg accaaattat tctgatgttc cttatgttaa
    10861 tggcatgttt tcaacaaaac aatatctaga tatgctaaaa actacttgga aagtatatca
    10921 aaaacgtttt aatactagtc taagtgacta tgctgctttt tgtttccata ttccttttcc
    10981 aaaattagct ttaaagggct ttaataaaat tctagataat aacttagatg aacaaaagaa
    11041 agctgaacta caagaaaatt ttgagcactc tattacctat agtaagaaaa ttggaaattg
    11101 ctatactggc tcattgtatc taggacttct atcgttacta gaaaatagtc aaaatttaaa
    11161 agcaggtgat caaattgcct tcttttcata cggtagcgga gctgttgctg aaatttttac
    11221 cggtcagtta gtagacggct accaaaataa acttcagagc gatcgtatgg atcaactcaa
    11281 taaacgtcaa aaaattacag taactgagta tgagaaactc ttctttgaaa aaacaatcct
    11341 agacgaaaat ggaaatgcta acttcaacac ctaccggact ggcacttttt ctctagactc
    11401 tatttgcgaa catcaacgta tctacaagaa aattaataat taatatgaca aaaatttcct
    11461 ggacaggatt ttcaaaaaaa tcccctgaag aacgtattca ttaccttgaa gaacaagatt
    11521 ttttagctga ttcttcccta gaaatagtga cgaatcagga cttactctct ttatcactag
    11581 ccaatcaaat ggctgaaaat gtcatcggtc gaatagcctt accattttca cttgtcccag
    11641 atgttttggt taacggtaaa gtctatcaag tcccatatgt cactgaagaa ccgtcagttg
    11701 ttgctgctgc aagttttgct gctaaaatta taaaacgatc tggtggtttt ctgacaaccg
    11761 tccataatcg taaaatgatt ggacaagtag ccctttatga tgtacaagat agtcaacaca
    11821 ctaaagaatc tatattaaat caaaaacagc aattattaga aatagccaac gctgctcatc
    11881 catcaatagt caagcgtgga ggcggtgctt gcgacctaac tattgagatt aaagaagact
    11941 ttttgattgt ttaccttatg gttgatacaa aagaagccat gggtgctaat atggttaaca
    12001 ccatgatgga agcattatct tcgccattag aagacatttc aaaaggcaaa agcttaatgt
    12061 caattttatc taactatgct accgaatctt tagtgactgc tacatgccga gttgatttaa
    12121 gatttctaag tcgccaaaaa gaggaggcca tcaagttagc tcaaaaaatg acgatggcaa
    12181 gtcaattagc tcaagtagac ccttatcgtg ctagcacaca taataaaggt atttttaatg
    12241 gcattgatgc tatcgtctta gctactggaa atgattggcg agctattgaa gctggagcgc
    12301 atacctatgc tgtcaaagat gggcaatatc gaggattatc tagatggtct tataaggttg
    12361 atgataattg cctagaaggt actctgacgc taccgatgcc tgttgcaaca aaaggtggat
    12421 ccattggtat taaccctagt gtccacctag ctcacgatct actaggtcgt cctaatgcta
    12481 aagagttggc aagtattatt ctttcgattg gactagctca aaattttgcg gctcttaagg
    12541 cattggttag cacaggaatt caagctggtc acatgaaact tcaagcaaag tcgttggcac
    12601 tacttgctgg agctaaagaa gaacaaattt cagaagtcgt taaacagctt ctagactcta
    12661 aacacatgaa tctagagaca gcacaaaaaa ttgtcaataa acttactaaa agtaaaaaaa
    12721 gaatttgaga aaaatcatct caaattcttt ttttacttga cttgaatttc cataagtgta
    12781 taatctagtt ataaagacta gattatatgg agttttaaaa tgaataaaaa tcaataccca
    12841 aaatgggaag aattacctga actagatttg tatcttgacc aagtacttct ctatgtgaat
    12901 caattaatta atcctaaaac aataactaac gataaactac tcaccgcttc gatgatcaac
    12961 aattatgtca aacataatta tatatccaaa cctataaaaa aaaaatacaa tcgccgtcaa
    13021 gtagcacgat tgattgttat tacagctttt aaacaaattt ttgctattca agaaatcagt
    13081 caaacattag aacttctgac agctgacaat cattctgaag aagcatataa cggatttgct
    13141 gcttgtatga acaaagaaga ggttcacgat ttaccaccag ttgtcattag tgcctgtcag
    13201 acgttgaacc tttatcaaga aacacaaaaa ttagttttag aactaggagg aaaaaataaa
    13261 aatgcttaaa gaaatgtcta tcaaattaag tcctcaactt tcctttggcg aagagcttgc
    13321 aaatagtatc actcacgctg taggagcttt gcttatgcta atcctattac caatcaccgc
    13381 tgtctatagt cacaaccact tcggattaca agcagcactt ggtacctcaa tattcgtcac
    13441 ttctttattt ttgatgtttt tatcatcaag tatttaccac tccatgactt ataacagtct
    13501 tcaaaaatat gttctacgca tgattgatca ctctatgatt tatattgcta ttgcaggtag
    13561 ttatactcct gtcgcattgt cacttattgg cggctggtta ggatatctca tcattttttt
    13621 acaatggggg attacattat ttggtatcct ttataaaatc ttcgctccca aaatcaatga
    13681 taaatttagc cttgtacttt acctcattat gggatggctg gttatcttta ttttccctgc
    13741 tattatcaca aaaactggac cagctttttg gggattactc ttggctggtg gtatttgcta
    13801 tactatcggt gcactctttt atgctcgcaa acgtccttat gatcacatga tttggcacct
    13861 ttttattcta ttagcttcta ttttacaata cattggaatt gtctacttca tgctataaaa
    13921 tttaactaca ttttattgat tatttttttg ctaaaccatg atataataat aatcatctaa
    13981 atcttagaga gaaggcgtgg acatatgaaa agtgcttata tcttttttaa tccaaaatct
    14041 ggtaaagatg aacaagcatt ggcaaaagaa gtaaaatctt atttaataga acatgatttt
    14101 caagacgatt atgttcgtat tattacacct tcatctgttg aggaagctgt cgccttagca
    14161 aaaaaagcat ctgaagacca tatcgacttg gttattcctt tagggggaga tggcactatt
    14221 aataaaatct gtggtggtgt ctacgctggt ggtgcttatc ctactatcgg actagtacct
    14281 gccgggaccg tcaataactt ttcaaaagct ttgaacatcc cgcaagagag gaatctcgct
    14341 cttgaaaacc ttctaaacgg tcatgtaaag tccgtagata tctgtaaagt taatgacgat
    14401 tacatgatta gcagtttaac acttggtctc cttgctgata ttgctgctaa tgtcacttct
    14461 gaaatgaagc gtaaattagg cccttttgct ttcttaggtg atgcctaccg tatattaaag
    14521 cgtaatcgat catattctat cactcttgct tacgataata atgtacggtc tttacgcacg
    14581 cggttactcc ttattaccat gaccaattcg attgccggta tgccagcctt ctctccagaa
    14641 gcaacaattg atgatggact ttttagagtc tacactatgg aacatattca cttcttcaaa
    14701 ttattgttgc atctaagaca atttcgtaag ggagatttta gtcaagcaaa ggagataaaa
    14761 cattttcata ccaataatct gactatttca acttttaaac gtaaaaaatc agctatccct
    14821 aaagttcgta ttgatggcga tccaggtgac caattacctg taaaagttga agtcatccct
    14881 aaagccttaa agtttatcat tcctaactca ttaccataat ataaaaggac ctagacataa
    14941 gtattagagt aaatactatc tgtctaggtt cttttttgtt atacttaaag caataaatat
    15001 cattgaaagg aagtatacga tgacaacatt tactgctaaa tttattgacc aagagtggga
    15061 agtcccagta gaatcaggtc gctaccatat gattgttggg gaattttgtc cttatgcaca
    15121 acgtccgcag attgcgcgcc aactgcttgg tttggataaa catatttcta tcagttttgt
    15181 ggatgatgtt ccaagtgata taggacttat ttttagccaa ccagaacagg tcactggtgc
    15241 taagtcgctt cgtgatattt atcatttgac tgacccgacc taccaaggtc cttatactat
    15301 cccaatttta attgacaaga ccgataatcg tattgtctgt aaggaatctg cagatttgct
    15361 tcgacttttt actacagatt tctctgattt gcatcaagag gatgctcctg tccttttcag
    15421 ccaagaaact gcttcactta ttgataatga cattaaagac attaataaga acttccaaag
    15481 cttaatgtat aagttagctt ttcttgataa acaagcagat tatgatactt atagtaagga
    15541 attcttcact tttctagatc aaaaagaaca cttactagga caacgtcctt tcttgctcgg
    15601 agataatcta agtgaggttg atattcattt ctttacacca ttagtccgat gggatattgc
    15661 tgggcgtgat cttctactac ttaatcagaa agcgctagaa gactacccaa atattttttc
    15721 ttgggctaaa actttataca atgactttaa tttaaaaaca ttaaccaatc cacaatccat
    15781 aaaaaataac tattacttag gcaaatttgg aagagctgtt cgtcatcata ccatcgtccc
    15841 aacaggtcca aatatggcaa aatgggaaaa ataaagctaa aaagctaagc aattcacagc
    15901 cttagctttt tagctatact agtccaaagt ggaccaatca aattcaataa aagtcttgaa
    15961 gagatctcca taagtaccac tgtaagcaat atctttatct ttataagtca ttttcgtaat
    16021 aggataattg tcaggaacag taacacatcc cattaaaact cgaataaaat catttggatt
    16081 ttcaaaaact tggcatcgtt cagtgtcgta agcatcttta taatacagtt taatcatctt
    16141 attacctctg acgttttaca aggaaacttt gacaggaagc tatatctact tcacaaaaat
    16201 aatatcattt agcattggct tccttcaatt gaccatacaa tatataattg acttgtcgta
    16261 aatcagttat ttttttacaa ttcagtgcac acattatcat tcttaaatct tctttccaag
    16321 aattgagaat tgcaataaca tcatctacag gataacgctc aactaattct aaaactgttc
    16381 tagaaagtcc aacagcttta gcacccaaca ctaaacattt taccatatct aaaggatgac
    16441 gaatgccacc agatgctagg atatccattt tgtccatcat agattgagcg tttattaagg
    16501 attgagctgt tgtttgcccc caggtattga gatacgaacg gtcgcgtcca cgttgatttt
    16561 cgatgtaagc aaaactagtc cccccacgcc ccgaaatatc tacagttgtg atacctatat
    16621 cgtaagcatc ctttattgat tgtaaatcca tcccaaaacc aacctctttt aaaataagtg
    16681 ggcattggat attgtcgacg tattctttta agtgagaacg ccacatgtga aattcacgct
    16741 ctccttcagg catcaaaagt tcttgcatca cattaacatg aacttgtaaa aaaatggggt
    16801 tcatagcctt aacggttgac tctgctgctg gtacaggctt gtctaaccct atatttgtcg
    16861 ccagttttaa atcaggatac aagtctgttg ttgggtagga atcatcttca tcgtttttga
    16921 gagctgccga atatgaacct gtcaccataa caatccctgt agcttgagca acctgtgcca
    16981 atttatgatt gactgctttc cctttttcac taccgcctgt catagcattg atataaaaag
    17041 gaaattcaaa gctttgacca gcaaaatgtg ttgataaatc gatatcgttg acattatatt
    17101 ttggtaagga tgaatgtatt aactcaatat cgtcaaaact attataatgc gactgatatt
    17161 taagagcata ttttatgtga tcatcttttc gatttgtcat gacagtgtct ccttactaag
    17221 tagttcaata ccagcactct cccaacgctt gattagagtt tgattatcat ttttattaaa
    17281 ggatatggct ataccacaat cgcctcctcc agatcctgaa gattttgtga ctatatctag
    17341 gcctttgctt gcctctttaa gcttttgaag tttatcggtg taaatagatg gatgaagatt
    17401 aaataatagc aagctaattt cttctaaaca tctcttaatc gcttctttat ctccttcttt
    17461 aaaggctgaa agtaactgga caacttgata ctttgtatca tctaaaaaac gctgcgtaat
    17521 agttgattta actatattta tcatttcttt tgaaatggac ggttgcattg tccatcccac
    17581 taaaaaatcc atctccaata aagcttgtat tttggtaata cgataacccc attcagcttc
    17641 caatacttgt tccaaaggtt ttgtttcaat catttttgaa acagccctac gatcaaaagc
    17701 tgaataagaa attaaatgct cataagcaat acaagcaata tctcccattg agccattatc
    17761 acctcttttt aagagaacat aggcagataa tttaaaaagc aagtcgcttg gcatctctat
    17821 ctcataaagc gcagccatag ctttaatagt caaaacgaca acacttccgc tagagcctat
    17881 accaaattta agtccatagc gctctaattt tcccgtaatt tcgaggtgaa tcggttttat
    17941 atttttgcca aaattaatca accattcttc cataagcaat atcgtttcct gaatgagact
    18001 atattgctta tctggctgta agcttgccgt atagttaaac atatcagaat aaaggctgta
    18061 gttatctgca aaagtagcta atgcagtcat ataaatagga acgttcttta aaatagctac
    18121 ttgcccagga tacagtatcg cgtattcacc tgcaatatat aatttgccac ctgtttgcac
    18181 cttaaccatc tgctaattcc tttgtcgttg aaacaataat acgataatct ttctccaaga
    18241 tagcagctaa tgcctcaagg tcttgacgta agcaaagtac tttaacattt ggacccgcat
    18301 ccatcgtata ataacattca tgaccttttt cgcgaagttt cttcacaaca tccatagctt
    18361 tatatgtctc ttctgtcaaa tatgaaaagg ctggagttgc tgttttagtt gttgagtgca
    18421 ttgcaagtgc attacgctct gtcagttgac ctaccttttg aaaatcatta ttttttaggt
    18481 atactaacat atcttgataa tcttgttcag attgccttac ccattcatta aaagtagttg
    18541 aagtttcggt gcagattttc ataccttcac ggcttgaaat cggctttctt ttgtcattta
    18601 agaccagcat aatcatagct aaatctaaat tagtgtgaac tttatagata tcaccactat
    18661 ctttatccca agcagcaaca ggtccaaaaa aagagcgtga agaggagcct gaagcaaact
    18721 tggcttcttg agctagctgt gattggctaa ggttagtacc aaaaaaatca ttgcaggcct
    18781 ttactaaggc tgataaacca cttgatgacg atgataagcc tgcagctgtt ggcatattat
    18841 tagttgtttc aatctttaca aagccggacc tattttgtcg aaagcgatcc aagatagctg
    18901 acattttgtc atgttcatga tcattttgaa gaacaccaga gatgtaaaat tcatctttct
    18961 tagcatcctt cccaagggca gtcaaacgtg tttcagtgta catgttttca agtgttagag
    19021 aaatactact ggtagctgga atcatttttt cagcatctgc ttttccccaa tatttgataa
    19081 tagcaatgtt agcataagac ttaacactta tactttttcc gtccatgtgt tgacagctcc
    19141 ttctctttct aattgctgac ttaagcgctc agcttctctt ttttctttta caagtgcgat
    19201 aatacaacca cctaatccgc cgccactcat tttagcgcct agagcaccac aactaatagc
    19261 tacctctacc agatgatcag cttctaaact tgagacaccc aattgtttta aactttgatg
    19321 tgctttagtc atcaagctac caagttgttt ggaatcactt ttatgaatag catcctctga
    19381 tgcctctgct aaataaccta attcttttaa aaagggtagt actgcctcgc cagatgattt
    19441 caccttatcc actgcttctt tagtatgccc atgaattcct gtatcagcaa taactaaata
    19501 agcattcaaa tgcatgggta ctgttgaaaa accaatattg cggataaatt taatagtatt
    19561 ttcactaaga caagtttttg catctaatcc actcggattt gaatgtgcta tcatttctgc
    19621 tcgattaaca aggcattcta acaaatctgc ctccaaatct tcatcaaaat aatcaaaaac
    19681 tgcacgaata gctgcaatag caactgcagc tgatgatccc ataccacgtc tttctgggac
    19741 ttgtgactca atatgatatg caattttaga agaagtttta cctaaatagt ccaaggcagc
    19801 aaaaatggct gtcgacaaag gatcagtcat atctaatgct attaattgag gagcctcttc
    19861 aataaggcaa gttacttcaa tattttttaa aggaatagca atggctggat aaccatatac
    19921 tactgagtgc tcacccatca agataatctt gctatgagcc tttcctatac caaacttttc
    19981 tttcatcttt ttctctttac aattctatta ccttactatt ttaatataaa tccttaaaaa
    20041 aagcgacttt aacgtgataa taacataaat taaaggaact acaattgcag tccctttaat
    20101 cttaaatgta aatgacaaag gtaatggtat catgtttata attcacatcg atacttcctt
    20161 taaatagatg caccaaacta gttacgatag aaagaccaat accatatccc gattttttgt
    20221 tattatgtga ctcatcctct cgataaaatc gttcaaaaaa cttgctatag tctatgtctt
    20281 taccattctt ataagtattt gaaacttcaa gcttagctcg tctcaaacga cttgaacgtg
    20341 ataacttaac tgtcacagtc cccataggat cacaatactt attagcatta tcaaccaata
    20401 atgtaaccaa ttcaaataat gatttttctt cagctttgac atgaatacct ggttggatac
    20461 tcattataaa gtctttacca tctttaatga ttggaccttt aaaatcttct gcagcatctt
    20521 ttgtaatatg tgaaaagtct aagtcaacca tacttatatc aggctgttct tcaaagcggg
    20581 caagactgac catgccatta attagacccg ttaaacgttg gatttgatca tttgtagatt
    20641 tagtccactc tgattcacca ctcatcattt ccaccaattc attattagcg gagatgatag
    20701 caagtggagt ttttagttca tgtcctgcat tggtaataaa acgcctttgc ttttcatagt
    20761 tagcaacaaa tggtaagata actctccctg ataaaactga aacaattacc atgaagacga
    20821 taaaacttac caaggacatc catattgaaa gttgcaataa tctttgatta tcaagatatt
    20881 ggttggttgt atctaaaaat accactaaat aatcattttt accaaccttt tttagtagat
    20941 aagaataagt actaaaatta taggtgatat gacctgaatg actattttgt gatacgatac
    21001 gcttggcata attttttatt tgcctatcgc ttacttcaaa gatatttcta gaatcaaaac
    21061 tagtcacgtt accttttgca tctgttcgcg cactgaaata acggaattgg aaaatagcgt
    21121 ccgttgatag gtcatcacct aattctttac taaattcaag tttattcggt atacgtccat
    21181 tgttgtctgc aaggatagtt aacacacgat aaatttcatt cttcgtttga tagtgattag
    21241 cagagttgag gacaccgact actgaaaata agacaacaag aatagctagc gcagctattc
    21301 cgataaaacg aagtcgtaaa tttctaaaca ttagccacca tttatttcac ttaatttata
    21361 gctactacct tcttgaccaa taatctccaa gttagcacca attgcctgca atttttgtct
    21421 caaataagaa acatagataa agatgtatcc ctcatctaca tcttcttggt ccttatctgc
    21481 tccccagaca tgttcgaaaa gttgttgtgt agataattct ttatcatggt tcaacatgaa
    21541 aaatgctaac attttagctt ctttacccgc tagacgaata gtgttttcac attgcaactc
    21601 ttgttcaccg actgataagg ttactcttcc caatgacaat acattaggtg tgaaatcttc
    21661 caatcttcgg gacatagagc gaagccttgc taataattct tttagagaga agggttttgt
    21721 taaataatca tctgcaccag catctagacc tgttactcta tcatcaattt ctgccatggc
    21781 tgttagcata ataatgtgag atttatttcc tgattgtcta atttctttga cagcctcaac
    21841 acctgtttta acaggcatca tgacatccat aaccatgaca tcatatgcat tttgattagc
    21901 taaatcaata gcagtctgac catcgtaggc gacatcaaca acataacctt gatgactaat
    21961 tgccgtacta aggacacgtg acatctgttc ttcatcttct gcaattaata ctctcatttt
    22021 attccttctt tgttccactc tctatcaatt gacgaatcgt ttgcatcgtt aaatcaacag
    22081 atgctatttc atcagcacaa cgttttaatt ctcttacaat tcgttcggga ttttcaatat
    22141 catgcttgta tttcatctga tgttctaaac tagcccaaga atcttgcgca attgtacgca
    22201 actgaatctc catgtaatat ttaccttctg aattacccaa acagtccgga tagggagtat
    22261 caatttctaa aatcacatgg taagagcgat acccatttgg cttaacatgc tggatatagt
    22321 ctttttcaac tacaatacga caatcatcaa atgtcttaat acgttctaca atttgataaa
    22381 tgtcgttaac aaaaccacaa ataatacgga ctccaatact atctctaata acttttagtg
    22441 cagaatctac tgttacaggt aactgtttac gacgacattt ctctatcata ctatctgatg
    22501 tcttaatacg accaatgata tgttcgtaca atttatagcc tgtttcaacc ttagctttat
    22561 cattttctaa ttggatacgc tgactgaaat cttctaaaat taatggtaga tatcgaccat
    22621 aatctccata aatatttgtt tccataagaa accttcctat tctctcttaa gctccattat
    22681 atcatagaaa cttaaggcaa actaaaaagg aatgagaatt ttttcttccc atttccttct
    22741 aaaatgttta tgttaagctg attcgaattg agaattatac agatcagcat aaaaaccact
    22801 ttgagacatc aaaacatcat gattgccttg ctcaatgata ttcccatctt tcatgaccaa
    22861 aatcaagtct gcgtttttaa tcgttgataa acgatgtgcg ataacaaatg atgttcttcc
    22921 ttccataagt ttgtccatag ctttttgaat taactcttct gtacgtgtat caacagatga
    22981 agttgcttca tctaagataa gtaagggaga atttttcaaa agagctcgag caatggttaa
    23041 taattgcttt tgtccaactg acaatgtcac agcgtcatca aggacagtat catacccttt
    23101 aggtagtgtc attataaaat gatgtatacc gactgcctta gcagcttctt gaacctcatc
    23161 atcactgatt gtcgtctggt taaaaatcaa attttcttta atagttccct caaacaacca
    23221 tgtatcttga agaaccatcg agaaagcatc atgaacttga ctacgtttca tgtcttggat
    23281 attgatgtca tctatggaaa tactacctga attaacatca taaaaacgca tcaataaatt
    23341 aacaattgtc gttttacctg cgccagttgg tcctacaata gcaactttct gaccaggctg
    23401 tgcctccgaa gtaaagtcat gaataatagt cttgttatca ctataaccaa acttgacatg
    23461 ttcaaaggac acatgcccct taaacttagg aagatttaac actttgtgtt tatcctcttg
    23521 catatttgtt tcatttaaaa attcaaaaac acgtcccatg gctgcactag cagactgtaa
    23581 ttgtgtaatt ccttgagcaa tctgaccaat aggctgtgta aaaatacgaa cataggtcat
    23641 aaaagcaaca attgtaccca tagtaattgt tcctttgata gctaacatgg caccaaccac
    23701 acacaccatc acataaccaa aattaccaat aaactgcatc agaggcatca taattccaga
    23761 gaaaaattgt gatttccaca tggaactgtg aagtttatca tttaatagtt gaaaatctga
    23821 cttagcctgc tgtcttgcat tatgactagt aacaatgttg tgtcctgagt aaatttcttc
    23881 tacatatcca gatatattag ccaaattcac ctgttgcttt ttaaataaag gctgactctt
    23941 caccataatg attgaagaaa gagcgaatcc tgaaaatacc gatataatcg ccgttgttgc
    24001 taaatgccaa tctgttttga acatcataat taatgaacca accaataaca ctgtagatgc
    24061 aaccatagta actaaacttt ggttaaaaga ctgtgtcatc aaatccacat catttgtaac
    24121 acgagataaa gtatcacctt gagtatggct atcaaaataa ctcaaaggta ttttattgat
    24181 tttatcagca atagcattgc gcataccttg agcaaatttt tgaataatag tcgtcacaat
    24241 aaaacttgct agataggata ctaatgcacc taaaacatat aatactgcca attgccctgc
    24301 aatatgaccg attttggcta agtcaatatt accagccaag ccttttacca taagatccgt
    24361 catctcttta agacggtctg gtcctataac tgttgtcaag cttgataaag ttgctaacac
    24421 aattgctaac gcaaaaagaa gtttgtaacc tttgaggtac ggagataaat tagagaaaaa
    24481 agacgttttt tgtgatttag ttttcatttt ccaattcctc cttagacaat tgagaatagg
    24541 ctatctcttg atatacttca ttatttgata acaattcctt atgtgttcct tgcccaacaa
    24601 ctttaccttg atctagcact aaaatgagat cagcatccat gatagttgaa atgcgttgag
    24661 cgacaattaa tttcgtcata gcagctgttt tttctttcag cacatggcgt aaaatatgat
    24721 ctgttttata atctaacgct gaaaatgagt catcaaaaat gagaatttca gggtgacgtg
    24781 ctaaagcacg tgcgattgcc aagcgttgtt tttgtcctcc tgaaaagtta ctaccacctt
    24841 gagcgacttc tgtatctaat cctttttctt tagcttctat aaattcttta gcttgcgcta
    24901 attcgagtgc ttgccaaatt tgttcatcac taagttcctc cctgccactc tctccaaaaa
    24961 tcatattaga acgtactgtc ccactaaaga gaacagcttt ctgtggaata tagccaattt
    25021 tattattcaa atcattgtga gagtattcct ttatatcaat gccatcaagt ttaattgacc
    25081 cctctgtggc gtcataaaaa cgtggaacta aattgactaa agtggatttt ccagaacctg
    25141 tagacccaat aaatgccact gtctgaccag tatctgcttt gaaagataca tgttcaataa
    25201 ccgcttcaga atctttacta taatgaaatg acacatcttg aaactcaatt tgaccttttg
    25261 gtgcactttg gatatgacta ttttctttaa aagttactgt tggggctaaa gttagtacct
    25321 cattaatacg tctagccgat actaaagcac gaggcatgat gataaaaata gctaccatca
    25381 tcataaatcc cataacaact tgcatagcat aagatgaaaa aacaaccatg tttgaaaaaa
    25441 gatccactcg atcagtgata gctgatttag cagccaatac agatacactt gttcctgtaa
    25501 ctttaggcac agaaatatca ttaatcaaat aagagccaat ccaataaata gctaaagtca
    25561 atccagatga aaccactgtc atcacaggat tcataagcga catcaatcta taaacaaaaa
    25621 ggttcaattt tgttaaatcg tcattttcac gtttgaactt atctgtttga tacttttcag
    25681 cattgtaggc acgaataact ctaacaccag agagtgcctc tctagtagtt gcattaagcg
    25741 catctgtaaa accttggatt tgacgttgtc tcggaaaagc aagtaagagt agaacgctaa
    25801 gtaatatcat aacgataata accgctactc caactgcccc agtccattga tcgcttttac
    25861 ccccaatttt agtcaaggcc catattgcca taattggtcc acgggtaaca acctgcattc
    25921 ccattacaat aaggatctgt agctgagtca agtcgttagt tgtgcgcgtt aatagagagg
    25981 ggatagaaaa tcgcttaatt tctgcatcag aataatccat cacatggtta aaaatatctg
    26041 aacgtaatcg actagtaaaa ctagctgctg tcctagcacc taaaaaacct accattactg
    26101 ctactaaaaa actaccaaat gaaaaagcaa gcatttttag tccaggatcc caaagagcct
    26161 ctaactttgt acctttagtt tgcaacatac gtgtgatatc agacatatat tctggagtct
    26221 ttaggtccat ccaaaccgct agacacacta atatagtact cgccacaatc atcaaccatt
    26281 ctttaccatt gagtcgcttg aaaatattaa acatcgcaaa ctccttttac tttcatattt
    26341 atcctattat cctaaaatag ttaatcaaaa tcttccatcg ccatattttt agctaatgtt
    26401 ctaataacct ttttggtagt acgtatctct tctttagtaa tacctgctaa catggtactg
    26461 tgcaattttt ctaagaaaat ttcaaattgt gtagcctttt gtttacctag atgagtcaaa
    26521 taaaggtatt tcacacgctt atctgtctta gatggaacaa tcgcaataaa tccatttttt
    26581 tccatccgtt ttaccaaatt agacgctaca gacttagaaa tgtgtaaaat ttcttcagca
    26641 tcttttatag acatatcctt gtcaggatgt ttatagagat gcatcactaa gtgtccttgt
    26701 ggacccgcta aatgttcaac atcatactct tttgcataac gatctaaata cttttcaagt
    26761 tgattaacaa gtattcgtgc tttttgaaga ggattctcca taataatact cctttaatag
    26821 ttatcgtgag aactatttta aaacaacctt tattaattgt caattaaaag ttaccgtgag
    26881 aactattttt acattaatat ttctaaaaaa cacctatcat tcgtcaaata aaaaatgatt
    26941 tcgcttactt tttaatatta aaaatatatg ttcgcttttt atatttttaa ttataaattt
    27001 atattgattt ttaatgtaaa tgatagtaaa atacaaatgt aatacttatt taatttacag
    27061 gagatttaaa tgaaaaagaa aattattttg aaaagtagtg tccttggttt agtcgctggg
    27121 acttctatta tgttctcaag cgtattcgct gaccaagtcg gcgtccaagt tataggcgtc
    27181 aatgactttc atggtgcact tgacaatact ggaacagcaa atatgcctga cggaaaagtt
    27241 actaatgctg gcactgctgc tcaattagat gcttatatgg atgatgctca aaaagatttc
    27301 aaacaaacta accctaatgg tgaaagcatt agagttcaag ctggtgatat ggttggagca
    27361 agtccagcta actcagggct tcttcaagat gaaccaaccg ttaaaacatt taatgcaatg
    27421 aatgttgagt atggcacatt aggtaaccat gaatttgatg aaggtttggc agaatacaat
    27481 cgtatcgtta ctggaaaggc ccctgctcca gattctaata taaataatat tacgaaatca
    27541 tacccacacg aagctgcaaa acaagaaatt gtagtggcaa acgttattga taaagttaac
    27601 aaacaaatcc cttacaattg gaaaccttac actattaaaa atattcctgt aaataacaaa
    27661 agtgtgaacg ttggctttat cggaatcgtt accaaagaca tcccaaacct tgtcttacgt
    27721 aaaaattatg aacaatatga atttttagat gaagctgaaa caatcgttaa atacgccaaa
    27781 gaattacaag ctaaaaatgt caaggctatt gtagtccttg ctcatgtacc tgcaacaagc
    27841 aaggatgata ttgctgaagg tgaagcagca gaaatgatga aaaaagtcaa tcaactcttc
    27901 cctgaaaata gcgtagatat tgtctttgct ggacacaatc atcaatatac aaatggtctt
    27961 gttggtaaaa ctcgtattgt acaagcgctc tctcaaggaa aagcctatgc tgatgtacgt
    28021 ggtgtcctag atactgatac acaagatttc attgaaaccc cttcagctaa agtaattgca
    28081 gttgctcctg gtaaaaaaac aggtagtgcc gatattcaag ccattgttga ccaagctaat
    28141 actatcgtta aacaagtaac agaagctaaa attggtactg ccgaggtaag tggcatgatt
    28201 acgcgttctg ttgatcaaga taatgttagt ccggtaggca gcctcatcac agaggctcaa
    28261 ctagcaattg ctcgaaaaag ctggccagat atcgattttg ccatgacaaa taatggtggc
    28321 attcgtgctg acttactcat caaaccagat ggaacaatca cctggggagc tgcacaagca
    28381 gttcaacctt ttggtaatat cttacaagtc gtcgaaatta ctggtagaga tctttataaa
    28441 gcactcaacg aacaatacga ccaaaaacaa aatttcttcc ttcaaatagc tggtctgcga
    28501 tacacttaca cagataataa agagggcggg gaagaaacac catttaaagt tgtaaaagct
    28561 tataaatcaa atggtgagga aatcaatcct gatgcaaaat acaaattagt tatcaatgac
    28621 tttttattcg gtggtggtga tggctttgca agcttcagaa atgccaaact tctaggagcc
    28681 attaatcccg atacagaggt atttatggcc tatatcactg atttagaaaa agctggtaaa
    28741 aaagtgagca ttccaaataa taaacctaaa atctatgtca ctatgaagat ggttaatgaa
    28801 actattacac aaaatgatgg tacatatagc attattaaga aactttattt agatcgacaa
    28861 ggaaatattg tagcacaaga gattgtatca gacactttaa accaaacaaa atcaaaatct
    28921 acaaaaatca accctgtaac tacaattcac aaaaaacaat tacaccaatt tacagctatt
    28981 aaccctatga gaaattatgg caaaccatca aactccacta ctgtaaaatc aaaacaatta
    29041 ccaaaaacaa actctgaata tggacaatca ttccttatgt ctgtctttgg tgttggactt
    29101 ataggaattg ctttaaatac aaagaaaaaa catatgaaat aataaaaata ggtttggata
    29161 aaatatccaa acctattttt atatcaatat gccttccaaa tgatctaact cgtgttgaca
    29221 aatttgagct ggaaaatctg tcaacttgat ttgctgttct ttccaattga tatcacgata
    29281 agaaatcgta atgtgatgat atcgttgtgt acttctgcaa cctacgagag aaaggcaaga
    29341 ttcttctgtc tcatatggat ccgattttgc aactaataca gggttaaaca ttactaaatt
    29401 tgtaataccc acattgatga taatgacacg cttcagacta ccaatcatat tggctgccat
    29461 acctacacaa taattttgat tagcatgcaa agtatcctgt aaatcttttg ctaagctcac
    29521 atctgctcta ctagccattt gtgatttttg ttgtaaaaag aaagtatctc taacaattgg
    29581 ttttatcatt tattttaacc taactctatt atgataatac tagtatacca taggttagct
    29641 aaggactatg ataagagatt aaacaatacc ttgtgcagac atagcttgag caacttttaa
    29701 aaagcctgca atgttagcac ctagaaccag gttaccacta tcgccaaatt catcagccgc
    29761 tttggaagca ttcacaaaaa tagttttcat aatacgttgc aattcttgat caacttcttc
    29821 aaaagtccat gccctacgac tagaattttg tgccatttca agcgctgaaa cagctactcc
    29881 tcctgcattg gcagctttag caggaccaaa agcaacccct gccttttgga aaactttaat
    29941 agcacccaag gtagatggca tatttgctcc ttctgttact gccaagacac cattttcaac
    30001 taataactta gcgtcttctt cgtctaattc gttttgagta gcacacggga aagctaaatc
    30061 tgctttaata gaccagatac taccttgatc tgctggtgtg aaattagctt ttggatgttt
    30121 ctccgtatat ttaacaatac gtgctctttc aacctctttt atctgtttca atgtttcaag
    30181 gtcaataccg tctggatcat aaacataacc tgaagaatct gacacagcta caacagttgc
    30241 acccaactct tgtaattttt cagtagcata aatagccacg ttaccagatc ccgaaaccag
    30301 tgcaactttg ccagttaaat cttgaccacg cgctgccagc atttctttag caaaatagac
    30361 tgccccataa ccagttgctt ctgtacgagc caaagagcca ccataagtca gtccttttcc
    30421 ggttaaaacc ccattttgat aaccatttaa acgcttatat tgaccataaa gataaccaat
    30481 ttcacgtcca cctactccaa tatcgccagc tggcacatct aaatctggtc caatgtattt
    30541 ttgaagttcg gtcataaaac tttgtgtgaa acgcatcact tcattatctg atttcccttt
    30601 tggatcaaag ttcgaccctc ctttgccacc accaattggt agacctgtca gtgaattctt
    30661 aaaaatttgt tcaaatccta aaaatttgac aatagattgt gttactgtag ggtgaaaacg
    30721 taaaccacct ttataaggac caatagctga tgaaaattgt acacgatatc ctcggttaac
    30781 ttgaacttgg cctttatcat ctacccatgg aactctaaaa gaaatcacgc gctcaggctc
    30841 aaccaaacgt tctaaaagat tttcttcaat gtactgaggg tatttatcaa aaactggtac
    30901 tagtgactca aaaacctctt caaccgcttg tagaaattct tcctcatgct cattttgctt
    30961 cttcactttc tctaaaacac ttgctacata tgtctttccg cttgtcattt attttcctcc
    31021 gtattgtttt aatatagtta gcattataaa ttgtcagaat tttcttgtca actatttgct
    31081 atggtatcat tattaggagc aattttaaag gtaattattt gacttttact gactttcgtt
    31141 ctataatgag acaaatattt gattggagta tctatatgaa atggttccca cttgttggag
    31201 gtataattgc cttattagct ggcttctatc tctttataaa tcctgtaacg gttgttgcaa
    31261 ctctgggttg gttcattgct ttatttatct ttatttatgg catttcaaat ttagtacgct
    31321 acttgcaagc tgattcttat caccgtagcc cttggtatct ggttcagagt atccttgcta
    31381 ttatttttgg tatcatttta ctatccagct cagcaatgtc actttcaaat actgtcatga
    31441 ctattttagc atactggcta ctggtatctg gtattttacg acttttgggt ggtttccaaa
    31501 tgcaaaaaat taatttccca ggtgccaaat cctataaacg ttcggcttat attgcactca
    31561 ttttaggtat tattttactg gttcaaccta ccttatcagc agccatcatt ggaagatttg
    31621 tagcctttat attaatagca attggagctt cggctattta cacttttact cgtattaatt
    31681 attaaaagga catgtttcgt gtcctttttt tatctattga tgacgtcatt tactatttga
    31741 aaattggcta ttatataatt cgtaataaaa tcctttatct gctaggagtt tctggtgatt
    31801 accttgttcg atgatttgac catcctttag aactaaaatg ttgtctgcct cttgaatcgt
    31861 tgatagacgg tgtgcaatga caaaactagt tcttccttcc atcaattttt tcatagcttt
    31921 ttgaataagc aattccaacc ttgtatcaac agatgaagtc gcttcatcta aaattaaaat
    31981 tttaggatct gctaataaag cacgtgcaat agtcaacagt tgcttttgtc ctaaggagat
    32041 attgcttgat tcttgattca tcaccatatt gtagccacca ggtaacgttc gaataaagtg
    32101 gtcaacattc gcagctttgg cagcagcaac tatatcctca tcactggctt caagattacc
    32161 gaatctaaga ttttctttta ttgtaccttc atataaccaa gcatcttgca acaccatgcc
    32221 aaattgttgc cggaaatctt ggcgtgatag gtgtctaata tcgtgaccat ctacggtgat
    32281 agcaccttca ctaacatcgt agaatctcat gagtaagttt ataagtgttg tcttacctgc
    32341 acctgttggc cctacaattg caatcatttg ccctggttct acatctaagt taaagtcgcg
    32401 gataagtggc ttatccggag aatagtgaaa atcgacacca tgaaagctga cttgaccggt
    32461 taagtcatgg ctcaattttt cagtgacttg attggcttcc tcttcttcat ctaagacttc
    32521 aaaaatacgt tctaaggaag attttgcact ttgcaaaaca cctgctaatt gtgtaatggt
    32581 ttgaacgggt tgactaattt gccaaacata ctgcacaaat gcctgcatgt taccaattgt
    32641 taaggtacca gcaataactt gtaaaccgcc aacaaatgca atgataagat aaataaaatc
    32701 agagatacta ttcaatactg gcatcataat tcctgaaatg aagctagctt tgaaacctac
    32761 atgacgtaga ttatccgtta tatcacgaaa ttcttgacta gacgcctctt ctctaccata
    32821 taattttatg atattgaaac cagttaattt ctcttgtaca aaaccattca gttctcccag
    32881 aatctttgcc tgttctttaa agtaaggttg ggatcttttt agtatcgctt gtgcactaaa
    32941 ataagttaca ggaatacaag caatgataat catggctaat ggtacattta gataaagtac
    33001 cataacgact actagaataa tacttaaaaa agcattgata atctgtaaaa agctctgttg
    33061 taaagcattt gagaccgttt caacatcact tgtaaaacgt cccaacatat ctccaaattg
    33121 gtgcttatca aaatacgata caggaacttt attgatttta cgattcaaat catgacggat
    33181 atcacggata cttttttgaa cgacagtagt cataaaataa tttgagccat aagaacctaa
    33241 ttcatagaaa acacctctga aaaaataaat aatcagaata ccagcaatat aaggaacgtt
    33301 caattctgcc ccagaaactc ccttagccat atcaacaagg ttagcagtca actctgttat
    33361 cgctaaccct aaaataaaag gctccagaat actcataaaa ctagataaaa ctttcaaaaa
    33421 aatcgctaag aaaagagtag ctttgtagcg agttaggtaa gaccataatc tcaaaaaaat
    33481 agattttttt ctcatacaat cactctcctt ctgataagtt ttggttcttc agttgtgact
    33541 cagcgatttc acgataaata gcattatttt ctattaattg agcgtgggta ccacgaccga
    33601 caatttcgcc ttcatcaagg acaataatct gatcagcatc cataatcgta cccacccttt
    33661 gagcaactat caaaaccgta gaatcaccgg ttacttcttt tagacgcgct cttaaagtag
    33721 cgtctgtctt ataatcgaga gcagaaaatg aatcatcaaa aatatataaa tctggatctt
    33781 taacaacagc cctagcaatg gataaccgtt gtttttgacc cccagaaaga ttgctcccac
    33841 cttcagctaa atgcgtttca aaggcttctt ggtgactctc aataaactct ttagcttgag
    33901 aaatatcaac cgcttgtcta agatcatcaa tagtagcatc agcttttcca tattttaaat
    33961 tctctcctat tgtccctgta aataaaagag ctttttgggg gataaatcca atcttttggc
    34021 gaagtgattt aaggttataa tctcttacat caactccatc tactaagatt tttccaagtg
    34081 tcacatcata aaaacgtgga atcaaattaa caagagaaga ttttcctgaa cctgttgaac
    34141 caataaaagc aattgtttct ccaggcttag ctttaaaaga aatatcatgc aaaacgggac
    34201 tctctgtttc tcctggataa gcgaatgtta cattatcaaa ttctaaatgc cctttaagtt
    34261 tcgtatccgt aacaccttcg gtattaggat taatagaaat cggcatatcc attacctctc
    34321 taatacgatg gctagatacc accatacgag gatacatagt aaaaagattg gcaaatagca
    34381 aaaatgaaaa gagagcatgg aagctatatt cgataaaagc aactaaatcc cctattttaa
    34441 tagcacctct ttgtaaggga tccaaagcaa accaaacgat agccacaatc attgcaataa
    34501 taatttgaac gaaaagtggt tctgttagcc ctgttaattt aaaaagacca gttgaagtat
    34561 ctgtgtaacg ttggttagcg acttgaaatt tttgtgattg aaaattctct cttgcaaagg
    34621 ccctaacaac gcgtaaccct gttaaatttt cacgaacata ttgattgatt ttatcaagca
    34681 tagtctgttg tctttcagat aaaggttttg tttttatagc tacatataaa atgactccta
    34741 ccaaaagagg catcgcaacc gctacaagcc aagccaaaga tggactcgta attagtatca
    34801 taaccacgct aaaaatcatt accataggag ttactaggcc taaacgtaaa gacatttcag
    34861 caaattgcat caaaacaaaa gtatcgcttg tcatacgtgt cactagtgaa gatacaccta
    34921 tctgttcata ttcatgatgg gagtattctt gaagcttagc atacatatca ttacgcatat
    34981 ctctaatcat tgttgtcgtt aagcgactag atgcgtaagt catcgtaata cgcccaataa
    35041 tacctagtac aacaaatata aacatgatga acgtccacag ataaactcca gtccgatcac
    35101 cttttgtaac gccattatca atcatacccg ctaaggctgt gggcattcct aggataaccg
    35161 tcacaaaaag catagctcct agtaaatcaa gccataacca atttgggtac cttttcaaat
    35221 aagaccaaat atatgccata tatgtctcct tttctttcct cttatctcaa aataattaat
    35281 ccatgttcac taatccactt tcaacttata aatctttaat catccaaata tccatagctg
    35341 tatgaccttg atcatttgct attggttgcg ataaggcaca atatcccata tgcttataaa
    35401 ccgcagttgc cctactcaaa atactggctg tctctaaata aagttgtcta tacccaattt
    35461 ttcgagcttc tacttcaatc attttcacta aatcagtagc aagcccctta ccacggaaac
    35521 gttctgcaat gtacaccttc tgcatctctg caattagatt tttcagcgga ccaaagccgc
    35581 ttcagccaat aatctcatct ctctcctcaa tgacaaagaa tcctgacttc tctatttttt
    35641 cgtagtatga ggtcaagtga tctaagtgag ggtctgaata tgctgtacca ggtttatcta
    35701 aatcataggc gcgtaaactc tgtcgaatta attgtgtaac tgcttggtca tcatttttaa
    35761 taatcggacg gataatcatg tgtctaaccc actaaggtac tcataacggt catacttttc
    35821 tagaagtgct tcattttttg catctaattc cttttgtaaa tcagataacc ttgtaaaatc
    35881 actaccacat gtttgcatat catcttctat acgagtgata gtattttcca atatcatcat
    35941 atcgtcttca attgtcgccc attcttgttt ttcaaagtaa gacattcttt tacgactttg
    36001 cttttcacga cttgtcttgg tactctcttt tttactgata acttcattat tttgctcatt
    36061 aaatgctttt tcatctaaat aatcagtata attaccaaaa aattcacgga tatcgttatc
    36121 ttcaaatgca ataattttat tagccacttt atctaaaaag taacgatcgt gactaactgt
    36181 aatcacagga ccaccaaagc cttgtaaaaa attttcaaga acagttaatg tagcaatatc
    36241 taagtcattt gtcggctcat caagtagtaa cacgttaggc ttttcaatca ggatttttaa
    36301 aaggtaaagt cgttttttct caccacctga taattttgca atttgtgttc catgtgtcga
    36361 acgtggaaag agaaattgtt ccaatagttc tgtcacactt gttgtaccga cactagtttt
    36421 aacctcatct gcaacctctt gcaaataatt aataacacgt tttgagccat ccatattatg
    36481 aagttgttgt gaaaagtaac ctacacgtat cgtttcacca atagagacat tacccgaatc
    36541 cggctgtaaa tcttgaacaa ttaaattaag taaggttgac tttccaacac cattatctcc
    36601 aacgatgcca atacggtctt tattttgaat taacaaatta aagtctttca agatagattt
    36661 atctgggtaa gaaaaagaga cattttcaaa attaataacc tttttcccaa ttcgacttgt
    36721 ttcaaatgtc atttccaaat cgcttgtatc gcttgtttgg tgtaaatcgt tttttagatt
    36781 ttgaaaacga ttaatacgag cctgttgttt cgttgcacga gcttgtggct gagtacgcat
    36841 ccaagctagt tcctgtttat aaagctgttt ctttttatgt aaactagcag catcacgctc
    36901 gtcttgttct gcacgaagtc ggacataatc ctgataattg ccttgatatt ctgtaatctg
    36961 tgccttatct aattcaaaaa tacgtgttgc aacattgtct agaaaataac gatcatgagt
    37021 tataaaaagc actgtctttt tactattttt caaaaaattc gttaaccatg caatagtgtc
    37081 aatatctaag tggttagtag gttcgtctaa gagcaataaa tctgcatcat ttaataatac
    37141 ttgcgctaat tgaacacgtc ttcgtaatcc tcctgataat tcactaaccg acaactgcaa
    37201 atcagtaata cctaatttgg ataatactgt tttgacttcg ctctcaatag accaagcatc
    37261 taaagaatcc atttctgcca ttactttctc tagacgtgat tgcttacttt cttcgtagtg
    37321 attaagcaat aattcatatt ctttaattaa agccatctct cttaagtcag aagaaagtac
    37381 ggtgtccaaa attgtctgag aatcatcaaa gtctggttct tgttttaaat aagcaatctt
    37441 ataatcatta gctgatgaaa aaggggaacg atcaccatca aaacctaatt cacccgaaat
    37501 aacatctaat agtgttgtct ttccagttcc attgacacca ataatcccaa tacggtctaa
    37561 actatggatg ataaatgaaa cattactaaa gaccgtctta tcaccaaccg acttagtcaa
    37621 tccatctact aaaaaatcgc tcatataatc ctctctaatc catcaagtac tgcttaacaa
    37681 aatgtaaaat agcctcttct tcattaatca gttgtcctaa aacaatggct aattcaattt
    37741 gagagataat atctcccatc tgtggaccag gttttattcc taatttcttg attaaggtgc
    37801 caccattgac aactatctca tgcctatcat ggatagctaa ctcactatct agttgttcta
    37861 ttttttcgaa atcaaccatc aagcctcttg ctttgcgaat accttccgct tgttttatta
    37921 tagtttttcc ataacgataa agatgttctt tagtcaatcc catttgttca cgaatacgat
    37981 aaacatccac tatatgctca acatcttttt gaaagtgagt tgatgtcttc cattttttta
    38041 aaaaagtctt tgtgtgttca acttccatac tcaagatgag tgatgcccaa gcttgttctg
    38101 atgtctcaaa taaaaaatct ttatctaact gggacaaaaa ggcgcttaat tgtaattccc
    38161 tattttttaa gcatggtaga taattaaaag catgactatc tattagtgcc agcattcctt
    38221 tacgccaata aggtgctaat agaagtttat caaattcaat aaaagaacgc tcaacagata
    38281 ttttctccaa taatggcgcg tgctttttca ttgcctcaaa agttgttgtt tcgatatcaa
    38341 aatttagact agcagaaaaa cgcaaacctc gcataattcg aagagcatct tcattaaaac
    38401 gctcactggc gagtcctact gctctaagaa ggtgattatc taaatcatcc aatccatgaa
    38461 agagatcaat aacttctcca tcttcattta aagcaaaggc attaactgtg aaatctcgac
    38521 gttttagatc ttcttctagc gacctaacaa aattgacctg actcggacgc ctataatcaa
    38581 cataaacctc ctcagtccta aaagtggtaa tctcatattc accacctttt tccaatacta
    38641 agactgttcc atgttcaata ccaacatcaa ctgtcctttt aaaaatctgc tttgtttctt
    38701 ctggataaga tgacgttgct atatcaacat catgaattgg acggtcaagt aaaacatccc
    38761 gaacactacc acctacaaag tatgcttcat aaccggcttt ttttatcttt tttaatattg
    38821 gtaaagcctt ctgaaactca gaaggcaaat aatttaatct catcatacta ttccaaatct
    38881 aaacgaacta aagtcgcatt attatttctt ataaatggat tcagaaacaa aaagaactgc
    38941 ctaagggaca ctccctacct actcaaatta tcaaaaaaga atctcctaac ttcttctttg
    39001 atatctgcaa taaggatcaa cctccaagca actcttttta tcacacatca attttttggg
    39061 gtaataccaa aggcaaccgc accttcacca agatgagttg caataacgcc accaaatgat
    39121 acaatttcta aatcgtcttt aagaccagct ttagctaaca aattatataa ttgttctgcc
    39181 ttatcttgtg ctctagagtg aataatagca atatcatatt caccatctgc tgtcatttct
    39241 ttgacaatct cagccaagcg ttttaaggct tttttctctg tacgcacttt ctcatagaca
    39301 acgattttac cttcttcatt aaaatgtaag accggcttga tacttaaaag gttaccaatg
    39361 atagctgaac cgtttgacaa tctaccacct tttactagat gattcaaatc attaacaaca
    39421 ataaatcctt caattttttc aatttgtgat tgtatcttgt taactatgac atcaaaatcc
    39481 atcccttcac gtgaacacat caaagcattt ctaactaaat tgccctgtgg tgctgaagtt
    39541 attttagtat ccggaaaagc aattgtcaaa tttggatgtt cttcaataag aaactgaata
    39601 ttctgccaga aacctgatat ccccgctgca atgaataaac caaggacgtg tgtataccct
    39661 tctttctcca attgacataa gagatcatct agttcagcta agctaggctg acttgtttta
    39721 ggtaattctt ttgatgccgc tagcttatca taatattggt ctaatgtcag attttgtccc
    39781 tcaatataag ttttaccatc aatgataatt ggaatatcta aaacatagac atcttcatga
    39841 ttttcgatag cctcaggcaa gtaagctgag gtatccgtaa taagtgctaa tttcattaac
    39901 tattaaactc caatgttact cctggtctat ctaaagcaat ttctgtaact tcataagtca
    39961 gacgtttaac gatttctaaa agaggagctg ctagattctc aacttcatct tgactaaact
    40021 cactaggctc attgataagg cgcccttgga tatgagctat ttgtgaaatg acaccagaaa
    40081 taacaaactc atcacgtaca ataacaaatt gtaacactgc tacaatgctc gttacttttg
    40141 ctgcttcatc tctattagct aattggaaag taacttcaaa atttgttttt ggggtaccat
    40201 tttcctcttc ccattctaaa ttgcgcgcat catagtggta ttgattgaca aattcttgtt
    40261 cacgaatgac ttccatgtat tttctccttg atagacaata tgtattatta tagcataaat
    40321 ttgtagattc ttaaagcagg tgggaatcta gaacttcttc ttaggatgtc tatcaatcac
    40381 ttcttgattg ttactcaagg ctgctaaacc tttttctgta agtgccaacc ctctgacaac
    40441 aaacaacttc cctaattcct cttctgtaaa agcaatagct attgcctgat ctaaatcaga
    40501 gcgtttcatc tttgacaagc cttttacgtt tttttctttt aagaatagtt ttaaaagtgg
    40561 agcagataag tgatctaaag aatcaagcgg tgactccatt cttacaaatc cattttttat
    40621 taaaaattct aggtgttctt ttccatttat accataaatg tattcaaaat attttgaaaa
    40681 acttgtttca gttgtgaaag ttccaaactg tatacgccat aataagataa tatctccagg
    40741 caagagtcct tcactagttt cttccatatt tttcctagga acaactttac taaaattaat
    40801 ttcagccaag tctctttcag gtgagatata ggggatatta agataatttt tgtaaatttc
    40861 ttcaatagtt ttcatagtca tattgtagca aaaaagcgct tataaaaaca agcgctaagg
    40921 catgggggaa gtcacctttt tgaaaaattc aatttcattt tctgatattc taaccatgtt
    40981 aaaattatca tgataacatc aaatacacat agaaaaatca ttaaaatgct atgactagaa
    41041 aaatattccc ttagttggaa aatgataaac ccaacgaaaa ctaaaattga taaagggtaa
    41101 gcaaataatt tctctttatg taataaaata agtgctact
//
LOCUS       DATRSG010000010        35346 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRSG010000010 DATRSG010000000
VERSION     DATRSG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35346
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.010"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(109..969)
                     /locus_tag="VBD89_000535"
     CDS             complement(109..969)
                     /locus_tag="VBD89_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7135088.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(1062..1580)
                     /locus_tag="VBD89_000536"
     CDS             complement(1062..1580)
                     /locus_tag="VBD89_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO7135089.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(1577..2332)
                     /gene="rlmB"
                     /locus_tag="VBD89_000537"
     CDS             complement(1577..2332)
                     /gene="rlmB"
                     /locus_tag="VBD89_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO7135090.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(2435..2821)
                     /locus_tag="VBD89_000538"
     CDS             complement(2435..2821)
                     /locus_tag="VBD89_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO7135091.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(2814..4157)
                     /locus_tag="VBD89_000539"
     CDS             complement(2814..4157)
                     /locus_tag="VBD89_000539"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO7135092.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(4154..4336)
                     /locus_tag="VBD89_000540"
     CDS             complement(4154..4336)
                     /locus_tag="VBD89_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135093.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            complement(4346..4930)
                     /gene="cysE"
                     /locus_tag="VBD89_000541"
     CDS             complement(4346..4930)
                     /gene="cysE"
                     /locus_tag="VBD89_000541"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO7135094.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(4939..5691)
                     /locus_tag="VBD89_000542"
     CDS             complement(4939..5691)
                     /locus_tag="VBD89_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO7135095.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(5693..7822)
                     /gene="pnp"
                     /locus_tag="VBD89_000543"
     CDS             complement(5693..7822)
                     /gene="pnp"
                     /locus_tag="VBD89_000543"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO7135096.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAESRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(8202..8471)
                     /gene="rpsO"
                     /locus_tag="VBD89_000544"
     CDS             complement(8202..8471)
                     /gene="rpsO"
                     /locus_tag="VBD89_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO7135097.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(8559..9614)
                     /locus_tag="VBD89_000545"
     CDS             complement(8559..9614)
                     /locus_tag="VBD89_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEO7135098.1"
                     /translation="MAVFSMILLLLHKIGLVQGGHGSEFAKTIGSAGLYLFLSIVFVA
                     YFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVLGYAVIGVISG
                     FYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTFLKIYQAGFES
                     AAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHMLFDKDKKEQV
                     WIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAKANPNFKLHLN
                     NSSLQGRLDFSQSVFEGQPTIFMCGPTSMTSTYAKVFRQKDAKSRLVYEGFSFRDSWL
                     SIFLLKTFDKVYSNLIK"
     gene            complement(9681..9818)
                     /locus_tag="VBD89_000546"
     CDS             complement(9681..9818)
                     /locus_tag="VBD89_000546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135099.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     L"
     gene            complement(9927..10856)
                     /locus_tag="VBD89_000547"
     CDS             complement(9927..10856)
                     /locus_tag="VBD89_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO7135100.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVEHCKSIYKS"
     gene            complement(10853..11710)
                     /locus_tag="VBD89_000548"
     CDS             complement(10853..11710)
                     /locus_tag="VBD89_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7135101.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            complement(11713..13068)
                     /locus_tag="VBD89_000549"
     CDS             complement(11713..13068)
                     /locus_tag="VBD89_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO7135102.1"
                     /translation="MKGLLDFLVNIARTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGSLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(13081..13365)
                     /locus_tag="VBD89_000550"
     CDS             complement(13081..13365)
                     /locus_tag="VBD89_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7135103.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(13368..15404)
                     /locus_tag="VBD89_000551"
     CDS             complement(13368..15404)
                     /locus_tag="VBD89_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO7135104.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLTAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETISLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            complement(15620..17245)
                     /gene="treC"
                     /locus_tag="VBD89_000552"
     CDS             complement(15620..17245)
                     /gene="treC"
                     /locus_tag="VBD89_000552"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO7135105.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(17467..19497)
                     /gene="treP"
                     /locus_tag="VBD89_000553"
     CDS             complement(17467..19497)
                     /gene="treP"
                     /locus_tag="VBD89_000553"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO7135106.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(19779..20405)
                     /locus_tag="VBD89_000554"
     CDS             complement(19779..20405)
                     /locus_tag="VBD89_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135107.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(20389..21192)
                     /locus_tag="VBD89_000555"
     CDS             complement(20389..21192)
                     /locus_tag="VBD89_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135108.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(21204..22025)
                     /locus_tag="VBD89_000556"
     CDS             complement(21204..22025)
                     /locus_tag="VBD89_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135109.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(22022..22999)
                     /locus_tag="VBD89_000557"
     CDS             complement(22022..22999)
                     /locus_tag="VBD89_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135110.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(23112..24740)
                     /locus_tag="VBD89_000558"
     CDS             complement(23112..24740)
                     /locus_tag="VBD89_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7135111.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            complement(24983..25711)
                     /gene="lrgB"
                     /locus_tag="VBD89_000559"
     CDS             complement(24983..25711)
                     /gene="lrgB"
                     /locus_tag="VBD89_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO7135112.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(25713..26168)
                     /locus_tag="VBD89_000560"
     CDS             complement(25713..26168)
                     /locus_tag="VBD89_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7135113.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(26338..27078)
                     /locus_tag="VBD89_000561"
     CDS             complement(26338..27078)
                     /locus_tag="VBD89_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO7135114.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASAELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(27059..28804)
                     /locus_tag="VBD89_000562"
     CDS             complement(27059..28804)
                     /locus_tag="VBD89_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO7135115.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVCALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(28831..29475)
                     /locus_tag="VBD89_000563"
     CDS             complement(28831..29475)
                     /locus_tag="VBD89_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7135116.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVTRDGVRPIKGAQQLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(29599..29994)
                     /locus_tag="VBD89_000564"
     CDS             complement(29599..29994)
                     /locus_tag="VBD89_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7135117.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            30075..30791
                     /locus_tag="VBD89_000565"
     CDS             30075..30791
                     /locus_tag="VBD89_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7135118.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRALPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNIIGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETDWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(30845..31471)
                     /locus_tag="VBD89_000566"
     CDS             complement(30845..31471)
                     /locus_tag="VBD89_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO7135119.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIAIEGVGQVTLSDQVIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(31504..31827)
                     /locus_tag="VBD89_000567"
     CDS             complement(31504..31827)
                     /locus_tag="VBD89_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO7135120.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(31824..32108)
                     /locus_tag="VBD89_000568"
     CDS             complement(31824..32108)
                     /locus_tag="VBD89_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO7135121.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREL
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            32269..32508
                     /locus_tag="VBD89_000569"
     CDS             32269..32508
                     /locus_tag="VBD89_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135122.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     ASFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            32693..33760
                     /gene="pepA"
                     /locus_tag="VBD89_000570"
     CDS             32693..33760
                     /gene="pepA"
                     /locus_tag="VBD89_000570"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO7135123.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            33830..34600
                     /locus_tag="VBD89_000571"
     CDS             33830..34600
                     /locus_tag="VBD89_000571"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO7135124.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAEINFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(34621..35286)
                     /locus_tag="VBD89_000572"
     CDS             complement(34621..35286)
                     /locus_tag="VBD89_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7135125.1"
                     /translation="MSFIIKKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKVLLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
BASE COUNT        11405 a         7218 c         5596 g        11127 t
ORIGIN      
        1 tttcataata tgtctcctac tattttatag tttcatagca tcatataaaa tacattttat
       61 aaaatgtata ctaaaagaac ataacgtact gttatgccct ttgatttgtt atcggttttc
      121 ttcccctaaa gagaatagag ctaaagcacc tgagcccaca tgagctgaaa taactggccc
      181 taatggacga ataataactt cttcaatacg ctcatctgct aataactgtt ctttcataac
      241 ttgagctgaa tctttctctc cactataagc aataattaaa gtgctataag atagtgtttt
      301 tgttgcctga gtcacaatct ctttaatacc ttttttacgg ccccttgtct tggcaaatgg
      361 tactaattta ccctcactat ctaacttaag taatggttta atcttagcaa cacttccaat
      421 gatagctgca ccttttgata agcgaccact acgcattaaa tggttgagat cgtcaactaa
      481 aaagtatgtg tttaatttag ggagaagtga ttcaattttt tgtttcactt cttctaaaga
      541 cttaccttct tgtcgctctt tagttgctaa catcgctaag acaccctcac cacatgaagc
      601 tgccattgta tccacaatct caatttgagc atcaggatat ttgtctaaga ccatctcacg
      661 cgcaatagtt gcactctggt aagtccctga taaatgtgaa gatagcgcta aatataacaa
      721 ggcatggtca ttttctgcat aggttgaaaa aacttcttca aactgaccaa cgttaatttg
      781 gcttgttgta ggctttgctc cctcttgcat acgctccaac aagaagtcac tagtaatttt
      841 ttcgtctccg actgtctcat aagtctttcc atcaagttca atggttaaac ctataatatc
      901 gacattgtgc tcttgagccc acttttcatc taaatccgat gtcgaatccg ttagtatttt
      961 aaaagtcatt gttacctcaa tctattattt ttatttgata atcaaagtag ttccaatatt
     1021 atatcaaaaa aattgttggc tggctaataa agtgttagtt tttacatacc atctaaaaaa
     1081 tccttgagtt ttcctagttg ttcatcattc cacggcctta actttggcgt actcaaatcg
     1141 atttgactgg acatcttatc caaatctgat tttactgtcg ctacacgctg ttccaattct
     1201 ctagctgaga cacgtaaagc accctgagaa aatatagtcc attgttcatt taaatcactg
     1261 gtagcaacgg agactaaatt taacacagat tgattaagtt ctgccgctgc tcgttcaata
     1321 tagctatccg cagtttcgtc ctcctcagta aagataacgg atattttgta ttggtcataa
     1381 cgttgacgta caccaggaac atactgggca tcaaatacac aaataatatc aatgtgttcg
     1441 aaatgtgcgt aatgatttag ctttctaagt aatacttccc ttgcctcttc taaacgattg
     1501 cttttaaaaa gctgtcgagt atctttccaa aaagcaatca tattgtagcc atcaacgagt
     1561 aaaatagaat gttttttcat aggcggtttc taaatacttc atacatcaaa atagcagctg
     1621 cgacactggc atttaaactc tgaacatgac cattcatggg aattgtaatc atttcatcta
     1681 cttgcttttt gatattatga gaaatacctt ttccttcatt accaataact agggctaatt
     1741 tacctttggt attccactta tgcgatggtg tcccattcat atcagtgcca aatatccaga
     1801 agtccttatc ttttaaagta tccaaagttt gactgagatt agttaccctt gcgataggaa
     1861 catgttcaac agctcctgta gatgtctttg aaaccaccgg ggtcactcca acagaacgat
     1921 gtttggggat gataatgcct gttacattgg ttgcatcagc tgtgcgtaga atagaaccta
     1981 ggttatgagg gtcggtcaga ccatcaagga ttaaaattag aggattttct tcattctctg
     2041 cctttgtcat tatctctgat aaatccgcat aggcaaattc cgaaactttt agaacgaatc
     2101 cttggtgaac tccaccattt gtcatgtctg ataagatttt tttaggagtc caggaaatgg
     2161 atactttttt ttcagtcgct agtgctttga ctttatcaac gttctttcct cttaagtcat
     2221 cttgcaagta taatttatta ccagtattag ctcttaaact ctctgtcaca gcatgtaagc
     2281 catagacaag gtcacttgat tcttctttaa attgtttatc tttcatagtc atattatatc
     2341 atgaatgcta tagcataaag ctttttatag caatatttta ccaacaaatc ctaatgtctc
     2401 tataaaaaag agaggatata gcctctcttt ttgtttattt ttcaatggtt tcaatacacc
     2461 actgtattag agtttccaag cgctttatct gaccagtcat atccaagtac cccataagag
     2521 cctcaaaacc tgtagacata cgataggtaa taatatcagc atttttagct tttgtatgac
     2581 tattcgcatt acgtcctcgt ttataaatta actgctcttc atcagttaaa atattttctt
     2641 ctaacatggc attaattaac aatgcttggg cattagcaga aacatattgg gttgctttcc
     2701 gatgtaactg attaggtttg gtaaaaccct gcataattaa atggcgtcgg atatacaaag
     2761 aataaactgc atctccttca aaagctaaag caataccatt aatcaaacga acatcaatca
     2821 cgagtccacc tcacgccatc cttagtatct aacaatttaa ttccttgttt tgctaattca
     2881 tctctaatac gatcagcagt tgcaaaatca cgattagctc gtgcttcttg acgttgttct
     2941 attaaagatt caatatcagc atcaagaact tcctcttgga atacaatacc aaaaatttct
     3001 aataattcag caagggtttc cttaactttt gaagtatagt gtcctgaatt aatccatttt
     3061 gccatttcaa agataacagt aataccgttt gctgtattaa aatcatcatc catcgcttct
     3121 tgaaatgctt tcacaaattg ttctaattct tcatcattag cattctcttg gattggtaag
     3181 ttgaaagtat tttttaaata tttaaggtta acttccgcat catgaacggc cttttcagtg
     3241 aaattcacgg gtttacgata ctgttgtgtc gctaagaaaa aacgtattac ttgtccgtca
     3301 acgctcttca acatatcgtg aacagtaata aagttaccaa gagatttaga cattttctcg
     3361 ttatcaacgt taacaaaacc attatgcatc cagtaatttg caaaggtctt acctgtttta
     3421 gcttcagatt gtgcaatctc attcgtgtga tgtggaaatt ctaaatcagc tcctccacca
     3481 tggatatcaa tagtatcacc aaggatctca gttgccataa cagaacattc aatatgccac
     3541 cctgggcgcc ctttccccca aggactttcc catgagactt ccccagattt agctgatttc
     3601 caaagtgcaa aatctaaagg attctcttta atctctcctt ccccatcaac acgaccactt
     3661 gcaccaatct ctaaatcttc caaagtttta ttggctaatt ttgcataatg atgtgattta
     3721 cttactctaa aataaacatc tccattagct tcataggcaa attccttatc aactaaaact
     3781 ttaacaaaat caataatctc atccatatag tcaataactc gaggattttt tgtagcaggt
     3841 ttgacaccta atgcagcaac atcttccata aatgctgaaa taaacttatc tgaaaaggat
     3901 tttgtatcca tacctgcttc agctgcacct tttataattt tatcatcaac atcagtaaag
     3961 ttagaaatat aattgacttg gtaaccacaa tactcaaaat aacgacgaat agtatcaaaa
     4021 gctactacac tgcgggcatt cccgatgtgt atatagttat aaactgttgg cccacagacg
     4081 tacatgttga ctttaccttc attgagaggt ataaaatctt gtaaactgcg agtcatagtg
     4141 tcgtaaattt ttatcatggt ttttcctttt tctgtttttg ataagcttta tacaaacgaa
     4201 caatatacat taaagcaaag gcagcccaaa taattcctaa gagagagtta ccttgtgcaa
     4261 actttaaata gcttaaacaa gctcctgcta gtatacctaa aattgataat atttgagttg
     4321 ttacataatc cttcacaaat acctcctaaa gtttggacga ataataactt tcttctcgat
     4381 catgttcaat acttcggatt tgaagatcat ccttttgacc atggacacgg acaactttag
     4441 ctggaacacc aactactgtt acgtctgctg gcacatctgc aaggacaact gctgcagcac
     4501 cgactttagc attttctcca acctcaattg gaccgatgat ttgagaatgt gctgaaatta
     4561 gcgcaccttt acgtatggtt gggtgtcgtt tgcctttgtc tttacctgtg ccacccaatg
     4621 taaccccatg gtatagcata gcacctttct ctacaatggc agtttctcca atgaccaatc
     4681 ctgaaccgtg gtcaataaaa accccttccg aaatcgtcgc tccgggatga atttcaattt
     4741 gtgtccagaa acgccaaaat tgactgtgca tacgtgctaa caatttaaaa ttatgattcc
     4801 ataaaaaatg agatagccta tgagctgcaa gagcttttat tcctggataa gttaaaataa
     4861 cttctagaga actacgagca gctggatctt gttctttgac aatagctata ctttctttcc
     4921 accaccccat agttttcctc aatcgttttc tacatcagac caatcaacta aaacttgttg
     4981 ttcatcaaat ggattgatgg ctactccaaa ggtatcatca atctcatttt ctccattacg
     5041 cggtttataa atatcgtcaa tcgtccaggc taaaattact ccttgtgctt tcctaaaagc
     5101 accaccaaaa tcattatggt tataccattt ttcaaaactt aaaaggtttg agaatactgg
     5161 aacataactt tttccttcgg gggtagacat tgtagggaaa aaacggtcaa aactaccttc
     5221 ttcacgtgga tggacaaagg caggtaccaa gtaggtttta tccatgatat cggcaacaat
     5281 gttatcttca tttaacaact gattcaagat tgtggtgtaa ttgttcataa attgcaccat
     5341 atcttcacag tagaaaatag tcgagttccc aaaatcgcct tcttttttta aattatagac
     5401 caatcctgct aagccatgtg aaatagcttc atcaagaacg tctaaactac gacgactctc
     5461 ccaaaacata tctcgagcac tttcttgctc ctctttgaaa tgattaaggt cagtaatatc
     5521 cgtaaaaaca ggaacaactt cttgaccatc tacttgaata gcatagggtt ccttgcttgc
     5581 ccaaaccgta gagtggtgca aggcattcac aagacctata ctatctaaaa aattatccgg
     5641 tgcattgata aaagcacgca atctaatgtc taactcgttt gtacttgtca tattaagatt
     5701 cctttttagg attgtcagct tttggaggac gtggtaataa tgctttcata gaggcatcaa
     5761 ctcgcccttt atcatcaatt ttgatgactt tcacatcaac ttcttcacca atttcaagaa
     5821 catcagctac atttgctgta cgtgtccatg caatttctga aatatgtacc aatgcatctg
     5881 ttttatcaaa gaggttgaca aaggcaccga atttctcgat acgaactact ttagcatggt
     5941 aaacttctcc cacttttgct tcacgtacta aacttgcaat aatgtctttc gtacgatcaa
     6001 tagcagcctg atcactagag aagatagata catttccttc ttcatcaata tcaatcttaa
     6061 cacctgtttc agcgatgatt ttatcgatag tttcgccgcc tttaccgatg acaactttaa
     6121 tcttatcaac atcgattttt atcatatcaa tcttaggtgc cgttggtgct aattgtggac
     6181 gtgactctgc gatagctcca tgaaggacat caagaatttc aaagcgtgct tttttagctt
     6241 gcgcaagagc ttcttccaaa atttgagggg taataccttc aattttaata tccatttgaa
     6301 gtgctgtaat cccttcacgt gtacccgcaa ccttaaagtc catatcgcca aaatggtctt
     6361 ctaatccttg aatatcagta aggactgtgt agtttgttcc atctgatatg agtcccatgg
     6421 caataccagc aaccggagct tttataggca caccacctgc cataagagca agcgttccag
     6481 cacatataga agcctgagat gatgaaccgt ttgattctaa aacttctgca actagtcgga
     6541 tagcatacgg aaactcctcc aagcttggaa ggacttgttc taatgcacgc tctcctaaag
     6601 caccgtgacc aatttcacga cgccctgcag ctccataacg tcccgtctca ccaactgaat
     6661 attgtgggaa gttatagtgg tgcataaagc gtttcttata ctctggtgtt agcccatcaa
     6721 tgatttgtgc ttcacccata ggtgctaacg ttaatactga taatgcttgg gtttgtcctc
     6781 gtgtaaagag acctgatcca tgaacttgtg gtaaaaaatc aatcactgca tctaatggac
     6841 gaatctcatc cacacgacgt ccatcaggac gaaccttatc ttctgtaatg aggcgacgaa
     6901 cttctgcatg ttccatttgt tctaaaattt cagcaacatc acgcataatg cgatcatatt
     6961 cttcatgttc agcgtaacgc gcctcatatt cacctataac tacttcttta actgcttctg
     7021 tagctgcttc tctagccttt ttctcttcaa cttgtactgc agcctgtaaa gctatgttat
     7081 gagtagcaat aatttctgct tgaagttctg gatcaacttg aagaagttct acttcggctt
     7141 tttctttacc aatagcagtt acaatctctt cttgaaaggc gattaattca cagacagcct
     7201 catgtccttt aagaagagcc tctagcatga tttcttctga aagttctttc gctccagatt
     7261 caaccatgtt aatcgcttct ttagtacctg caaccgttaa ttctaacgca gaagcctctt
     7321 gttcttgtgc tgtcggatta ataataaaat tgccatcaac ataagcaact tgaactccag
     7381 caattggccc attgaaagga atatcggaaa tcgataatgc taatgatgaa ccaaacatag
     7441 cagccattgg tgcgctagca ttttcatcaa atgaaagaac agtattgatg acctggactt
     7501 cgttacgaaa tccctcagca aacatcggac ggattggtct atcaataaga cgtgctgtta
     7561 aggtagcatc cgtacttgga cgcccttcac gtttgttaaa accacccgga aatttcccag
     7621 cagcatacat tttttcttcg taattgactt gaagtgggaa aaaatcacct gttgacattt
     7681 tcttagacat aactgctgct gtaagtacag tagaatctcc ataacgaacg acaactgatc
     7741 catttgcttg tttagccact tggccagttt caacaactaa ttttttacca gcaaaaatca
     7801 tttcaaagac ttgttttgac atattattct ccatattttt tgtttacgcg tttcctacaa
     7861 agttcaatct agctgttcta aactcaactt tgcataaaac cacgtataag ctttattcta
     7921 acataattct ataaaattga gtagcgaaaa tccgtggggg aagctgtatt atcattcatt
     7981 aatgtaaacg taaaaagtct gattattaat actagcaatc agaccttgtt acaatgatta
     8041 aaataaattt ctagggttaa tcttagcttg cttaataacc ctcttcatag gttaaaacac
     8101 tttcttactg ttggccccct caaagtactt acgttcatag tacgtttaaa acaaaaaaca
     8161 ctagaaaatt ttctagcgtt tttacttgac tctgttgtct attaacgacg aagtccaagt
     8221 gattgaatca attcacggta acggttaaca tctgtacgac gtaggtatgc taataagtta
     8281 cgacggtgac caattttttt catcaatcca cggtaagtag cgtggtcttt tttgtgttgt
     8341 ttgatgtggt cattaaggtg gttgatttcc caagtaagaa ctgctacttg aacttcaact
     8401 gatcctgtat caccttcgtg acgtgcatat tgagcaatga tttcattttt tttctctttt
     8461 gagattgcca taatatattc tccttttatt gcttcatccg agtcttagga ttggcactcc
     8521 taaaaccaag aaaaagttgg tttgtcttat agaccctctt attttatcaa attagaataa
     8581 actttgtcaa aagtttttaa tagaaatatg gataaccaac tgtctctaaa ggaaaatcct
     8641 tcatagacta aacgagactt tgcatctttt tgccgaaaga ccttagcata agtagatgtc
     8701 atactagttg gcccacacat aaatatcgtt ggttgtcctt caaaaacaga ctgactaaaa
     8761 tcaagtcttc cttggaggct actattattt aagtgaagtt taaaattagg attggcttta
     8821 gcataggatt ctaacatatc ttgataaatc aaattgtctt gatttgaaaa tgtataaaag
     8881 aagtcaactc tcttagtcaa aatactattt tctctaataa atgaaatgaa gggagtgatg
     8941 ccgatacctc ctgcaatcca aacctgttct ttcttgtctt tatcaaataa catatgacca
     9001 taagctctat ctaaggctat ttttgtccca acttttagct gtttgtaaat ggatttcgta
     9061 taatctcctg aagctttaac agttaaaaat atcactctat catgaccacc cgatatcgag
     9121 aaaggatgag ctgctgattc aaagccggct tgataaattt ttaaaaaagt aaattgtcca
     9181 tagtcatatc gataaggacg cttcattgct atttcaatct cagttgtatc atggttaaga
     9241 tgagtaacct tttgaacgta ccctacacga cgaaaacgca ttctagaata taggaaaata
     9301 atataaaatc cagatataac cccaatgaca gcataaccta atacgattaa actgagtaaa
     9361 gtattcccta gtatcctatc tcctaaaatc agaaaagtat gtaccaatcc caaaatatag
     9421 actaaataaa caaagcgatg aataaaacgc caaatttcat actttaaaaa atttccgaaa
     9481 taagctacaa aaacaatact tagaaaaaga tataatcccg cactgccaat agtctttgca
     9541 aactcacttc catgtccgcc ttgtacaaga ccaattttgt gcaatagaag caaaatcatt
     9601 gaaaatacag ccattacctt gtggtaaaaa tacatatttt caataccttg aaagtaactt
     9661 tcaagtatcc taaatttggt ttataagagg aatgttagcg ataaacttgt caaagctaat
     9721 cctggtacaa caaattcact taatcctgca ttttgccatg ctactagtgt caccactatg
     9781 ctagataaaa ttaaaacaat acctttccaa gctcgcataa cgaccacccc tttattttta
     9841 gtaaggataa aataccacaa gatacttata caaaacttaa aaatttcaga aaataacaaa
     9901 aaggcaactc caatgttgcc tttttgttat gatttataaa ttgatttgca atgctctact
     9961 atatctttaa cagcgagtcc atactcctct aataaatatt ccatctgacc tacctgacca
    10021 aatcgctcat caatacccat acgactaaca gtagtgtctt tctccatact aagccattcg
    10081 cataatgcac ttccgatacc gccaatacgg ttatgattct caatagttac aatggattga
    10141 tctatcagta atggtttcag ttcttctgga aggggcttaa ttttaaaaag gtcgataact
    10201 gaggcctcaa tccctagttc tttgaggtag tcggctgctt ctatcgctct agaaaccata
    10261 ataccacttg ctacaagagc aatatctttt ccctggcgta attgtataaa accttttgaa
    10321 aaatcttcgc atccctcata aactgcagtg ggggccttac gaatagttct aatgtatttt
    10381 agaccatcaa tacttaatgt ttgttttaaa attgcttcaa actgaatatc atcactaacc
    10441 tcaaaaatag tcgcctttgg aattaacctt agtagaccta actcttcaaa aggcatatga
    10501 gttccgccat tcatttctgc gctaactcct gcatctgagc caataatcgt agctgataat
    10561 tgtgagtaac ctaacgataa aaatacttga tcaaagacac gccttgacgc gaaaggacca
    10621 aaagtatgaa ggtatggctt gtaaccttta atggcgagcc ctgctgcaag acctaccatc
    10681 tcagcctcca taatacctag attaatataa cgttttccaa attctgatgc cagtacattt
    10741 gttgacattg aactcgacaa atcggcttct aaaacggtaa tttgtttatt ttcttgatta
    10801 gcttgaagga ggaaatcacg atagacaagc ctcatttctt tggttgaaca tctcattcta
    10861 ccacctccaa actttctctc aagcttatca ccgctcgctc taacattgtt ttttgttgta
    10921 aatctggtcg taaatgatga ttagaagcta actcctccaa ctctttcaca ccttgtccct
    10981 tgatactatc taaaacaatg cattttggtt tgacactatt tgaatcttga aaagttttaa
    11041 tagctttgtc aattgcctca atgtcatctc cttttacacg tactgcatca aatccaaaag
    11101 cttcaaattt agcaacaaag tctccaggat tacaaatatc agctgtcaaa ccatctaatt
    11161 gttttttatt atcatctaca aaaacaatta aatggtgcag ttgatgatgc gcagcaaatt
    11221 gtatagcctc ccaacattgt ccttcattta attcaccatc acctacaata gtataagtat
    11281 aatagcttga attctcaatc ttttgagcat aagcaattcc cgttgcaata ctaatgcctt
    11341 gacctaatga gcctgtcgtt acatctatac caggagttaa attgcggtca ggatgcgaag
    11401 gtagtttggt accgtttgta ttgagcgaat gaaggaatgt tttatcaaaa aaacctttta
    11461 aatagagtgt actatacaag gctggaccag catgtccttt tgataagacc atataattcc
    11521 gatcactttc tttgaatttt tcagggttta tatccataat atcaccgtac agaatagcca
    11581 aagtctcaac aattgataaa ctacccccat agtgaccgaa tcctaaatgg tttagcgttt
    11641 ctagtgtatt taaacgaatc tccgttgcaa atcgtcttag agatgaatat ctttcttctg
    11701 gtgatgtcat tattaacctt ccttatgggt tgattttcca acaaatgaaa tgccaaaaag
    11761 tccaattaag ataactacaa taccaataac aatagctatc gcaccgccga catgattaag
    11821 agcgccaaga atgataccgg tcaaaccaaa atcagcatct gagaatgttg aacctttgaa
    11881 accaagtcca cctagtacag gcatcaagaa aatcggcaag aaactgatga ggataccttg
    11941 aacaaatgca ccaatagtcg ctcctctcac accaccagaa gcattaccga taacacctgc
    12001 cgttgcccca cagaagaaat gaggaacgac ccctggtaaa atgacagtag tgccagtcac
    12061 aatcataact atcatactaa ccaatccacc aacaaaactt gagataaaac caatcaatac
    12121 tgcattaggt gcataagggt aaacaattgg acaatccaag gctggttttg aatttggaac
    12181 caatttttct gagattccct taaaggctgg tacaatttca cctaggataa ggcgtacacc
    12241 tgctaaaata acgaatacac ctgctgcaaa ttgcccagct agttggaggg cataaacaag
    12301 accattgaca ccgttactta tttcttttgc aatataagcc tcaccagcaa acactgcaac
    12361 gataaggtaa ataattgcca ttgagatagt aatacttacg gtactgtcac gtaaaaatga
    12421 taaactcttt ggaaatttaa tatcttctgt tgattttgat ttatctccaa caattccacc
    12481 aataaatcca ctcaaccaat agcctaaaga accaaagtgc ccaagtgcca ccttatcatt
    12541 acctgtcagt tgaatcatgt atttttgcac aaatgcagga gatacgctca taataatccc
    12601 taacgctaga cttccaaaaa ggatgagcga gaacgaagta aagcctgcta cggcaaaaat
    12661 aacagcaatc atacaagcca tatacaatgt atgatgtcct gtcagaaaaa tatatttaaa
    12721 ttttgtaaaa cgagcaatta aaatattaaa aatcattccc gctaacataa tcaaagcagt
    12781 tgctgagcca tacttcgtaa gagctactgc tacaatggct tcattattag gaactacccc
    12841 caccaaatga aaagcatgtt caaacatttt tccaaatgga ttcaaggaat tttggactat
    12901 ccctgcacct ccagaaacca ctaagaagcc aacaaatgtt tttattccac cttttacaat
    12961 atcaggaaca ccttttttct gcagtactaa accgataatg gctatcaagg cgactaaaat
    13021 agctggcgtt ctggcaatat taactaaaaa atccaataga cctttcatgg catcaacctc
    13081 ctagattaag cctttttctt ggcaaatacc tgtcactaat tctctcaatt catccatatc
    13141 aatgatactg tttaaaatac gaacatcccc caggtgacca gctgaatcct ccaaatcacg
    13201 accaacaatc caaacatctg ctgcgcttgg atctgctcca cctaaatcat aatgttcaac
    13261 ttcaacatca gaaaccccca aatccttcaa aatagactca atattcattt gtaccataaa
    13321 acttgaacct agacctgatc cacatgctgt tccaattctt aacatcttta atcctcctgt
    13381 tttataatca tttcaatatc ttgataccca cgtgaagcta acaaagcttt aacattatta
    13441 ttatcacgaa gtattgttgt caaatgcgat agagctttca aatgcgtttc attatcaata
    13501 gctgctatgc ataataagag ataaatttcc tgtttggggt catccaataa ataaataggc
    13561 tgttccaaaa caagcattga cataccgaca gagtttaccc catcttctgg tctggcatgt
    13621 ggaatagcaa tacctttacc aagattgata aaaggaccaa actcttcgac cttttcaatc
    13681 atagcttctg ggtaactttc cgtaattttt ccagaagcta acaagggttt tgccgctaaa
    13741 cgaattgcct ctttccaatt catttgttca gaacttgtct gataagtttc ctctgttata
    13801 agttggtgca acaagggcct cacatccttt ctaaatatat cttgttttac cattgtcctc
    13861 aaagccaatt ttaattcctc ttcacaatga acatgagcgt atttttttat agttgctatt
    13921 aattgatcaa gttggaaatc gtcaaggcaa gctttgggaa aatctgaaat aaccagttct
    13981 ttgagctgtt gtacttgctc tgctgtcatc atcagcgaaa ctagataatt aggttttttt
    14041 gtttctacaa atatagtaga aaagaccata tcgtaagttt gattgtcaat cagtttcagc
    14101 tgctcaattt tagagacacg atgaaaatga atttgaggaa ataatccacg caatttttcc
    14161 ttaatcacta aggatgaact aacgccgttt ggacagagta tcaaagcttt ataagacatt
    14221 gattgtgtat cacctgattg tctaagatag cctccaaagt gaatcacgaa gtaagaaatt
    14281 tcactatcgg gtattaatcc tacttgctct tccaaaggac gtagtgcctt tttaaccaat
    14341 agaaataaat cactgtaatg ctctttaata ttttgtgtat aaccactatc gccagttaaa
    14401 ccatatttca atcggaaata agctggaata atatgacgct tcagaccttg taataactct
    14461 tctttattgg aaaaattaag aagtgaaata gtttccatct catcaacgat tgcttttgcc
    14521 aatgcttcaa agaaatcatc atctttagtt ccctcacctt caaaacagcc agataagatc
    14581 aaggtaatat aatctttttc tttctgatct aatggtttag ataaggaaat atcctgactt
    14641 aaattagtca caataatagt cgttaactct tttaattgtt cagaaacaat aggagaaccc
    14701 tgcaaaacac gatctactga cctttgataa cgacacagta ttaaaatgat aaaatataaa
    14761 gatttctcaa gcctatcttg tattggagaa agttgaaaag attcatagaa atattctacc
    14821 gtcttttcta acttctcata tgagagagcg aatttccaag aagacaagat ataattcaaa
    14881 gaccaaaaac caatgggaga ttctagtagt tgagttatca tctgaaaagc aacttgatgc
    14941 ttatccatct catctcctac tagactatat ccctttgctc ttgtataagt gagtgagata
    15001 cctctttttg ctaatttaga acgtagcatc ttgatatctg ataaagtagt attcttactt
    15061 actttcaaaa aatcttgata atgcacattg gagacaaact ctcttcggca aaagctataa
    15121 aggtaaatca gacataccct ttcttcctga gttaggtaaa attgctcagc ttctaataag
    15181 gagacaattt tatcatgctc tttttcatct cctaataact gataacgtcc agcagagtga
    15241 gcaatagctg ttaggtgatt ttttgctaaa aaactattga gatagttaat ttgctctatg
    15301 ataactttta ttgaaaactt tgtttgcact gttaactcat acaaggaaag atttttccga
    15361 gctaaaaata ccaacattat cacaacagtc ttattatcaa ccatttgttt tcctttctat
    15421 ttttatttta tagtaaccgt ttacattttt ctaggtcatt tgagcccaat ctggcactac
    15481 tatcaattga atgaatttgg gctattgtta tagagtaaag aaaaaagaaa gaaattgcaa
    15541 aatctagagc aagattcctt cccacaagca gcacttaaaa aacctttact taattgaata
    15601 agtaaaggtt ttttaaatgt tatgacacta agatagctaa agtttgataa ggttgcaaag
    15661 taaccgtttc atcaagagta acatccttat aatttgatag aagaacttgt ccttggagat
    15721 aattctccgg taattttatc ttaactttct cagcaaagaa attatttaaa acaagaagtt
    15781 tttccttatc aagatggcgt tcaaaagcat atactttttc gttatctttg aaagctgctt
    15841 tataattccc gtcagctatt attggcagtt gtttacgtag ccttatcaac tcttgataga
    15901 aagtaaatat cagccctgtt ttctcctttg cgacattgat ttctttatat gattttccta
    15961 ctttcaacca tggagcgcct tcagaaaaac ctgcattcgt actatcatcc cattgcattg
    16021 gtactcgaga attgtctcga gatttcgctc gaataatgct aaatgcctct tcttgtgact
    16081 tgccttcatc aagcattatt tggtaggcat tcaaactctc aatatccaca taatcatcca
    16141 tagaactgta atcgggatca agcattccga tttcttcacc catatatata taaggagtac
    16201 cacgcgataa gtggatagat gctgctaaca tggttgctcc ttcatttcga aaacgtttca
    16261 cgtctacaaa acgatttaat gctcgcggtt ggtcatgatt attatagaat aaagcattcc
    16321 aaccatttcc ttcgctcatt ccttcgcccc aactatggaa aaggtcacgt aatgcaggaa
    16381 aatcaaaagc cataatggtc cacttttgcc catctttata atctactttg aggtggtgaa
    16441 aattaaaagc catggataac tcttcacgtt ctggtgcagt gtaaagaatg caatttgcaa
    16501 ttgtcgttga agacatttca ccaactgtca tgaaggaatc agcttgtcca aaactagcat
    16561 tattcaacat cttgagataa tcatgtgtta ttggacgatc agtataagct ggttttccat
    16621 cattaattgg gcagttctca agtatctcat ctttcccaat taaattaatc acatcaaatc
    16681 gaaaaccttt aaccccttta tcacgccaaa aattgacaac tttaaaaagt tccttgcgaa
    16741 catcggcatt gcgccaatta aggtcagctt gagtgatatc aaaaagatga agataatact
    16801 tccctgtatc accaaatggt gaccaagcat ttccaccaaa tttagatacc caatctgttg
    16861 gattatcacg taagataaag aaatcttgat agtagcgatc tcctgcaagt gcttttttaa
    16921 accactcatg ttctattgaa caatgattaa gcaccatatc aagcataaaa tctatacggt
    16981 attgacgtcc cacctcaatc atttcttcaa aatcgtccat cgttccaaaa tcagggttaa
    17041 tggctgtata atcagagata tcatatccat tatctcgttg tggacttgga taaaatgggt
    17101 tgagccaaac catatcaata ccaagttctg ctaaataggg taatttttcg ataataccac
    17161 gaagatcacc aaccccatta ccagttgtgt ctttgtatga ttttggataa atttgataga
    17221 caactttacg tttatcaatc gtcatattct gctcctttcc caaaatgctg ctgtatttag
    17281 cagtctaact ataacatcaa ctatcatcaa aaatataaca tttttacagc gatatttgtt
    17341 cccatgagtt ttttcttatg ttcaaaataa ttcaaggagc taatgtatta agcccctcaa
    17401 acaattcctc aaattacctc tcattttata agagtgctct tttttaaaaa ggatacctaa
    17461 ttacttttaa attttcttgg caaccattaa tgcttgtcca cgttttatct gtttgggtaa
    17521 atctatcaat tcttctggtc ggaattcttc ttgattagta atcagaatag gagtttccac
    17581 aatgtatcct tcttctttta tcattggaat atcgaaagta atgagtttat ctcctacttt
    17641 tacattgtca ccttgggcaa catgagaggt aaaccctttt ccttctaaat taacagtatc
    17701 cataccaata tgaataagaa actcaacacc ctctgatgtt aataatccaa tagcatgttt
    17761 cgtagggaaa agaactgaaa ctgtcgcatc gaccggtgaa actagttctc catcacttgg
    17821 atcgataacc actcccttcc ccataattcc ttgtgaaaag acaggatcac tagcttgatc
    17881 cagtggttta gctaaacctg ataaagggct tactacacta agctttgtac ctgagacgac
    17941 agctttttct tttgctgatt ttgtttctgt tgttgaagca atgacagcat cagatactaa
    18001 tttttcctct tcagtttttg tcaaaatacc tgatttttta aagaatagtg tcaaaaataa
    18061 aggaataaag atagccactg ccatacagat aaagaaatat cccatatatt taacattaat
    18121 tgacaggaag cctggaagac cgcctacacc aatagagtta gcttggacat taaaagttgt
    18181 cgccaaaaga cctgccacac ttgaaccaat cataccagca acaaatggat aaatgtattt
    18241 aacgtttaca ccaaaaagcg ccggctctgt aacaccaagg taggctgaga tagcagctgg
    18301 aagtgatatt tgagcctctt tttcatcatg acgatgcata aaataataag ctagtacagc
    18361 tgaaccctga gcaatatttg aaagagcaat catcggccaa aggccagttg tatgtgtttt
    18421 tgtatctgct atgagttgag tatcgatagc atttgtcatg tggtgcaaac cagtaataac
    18481 aaatggggca tacagggcac caaaaatagc accaaagagc cattttacag gaccagtcaa
    18541 tccaataaga acgatagcag aaatccattt acctagtgtc caaccaatag gtccaaggac
    18601 agtatgagcc aagataatag ctggaactaa ggatagaaat ggcacaaaaa tcattgaaac
    18661 aacttcagga atatgttttc tccagaagat ttctaagtaa gacaggctca aaccagctaa
    18721 cagagctgga ataacttgag cttgataacc aattttttgt actgtaaagt aaccaaaatt
    18781 ccaagaccaa tttttagcaa tgtctgctgc tgaagtacta gctacagaat aagcattaag
    18841 taattgtggc gacactagac agatacctag aacaataccg aggatttgag tagtccccat
    18901 cttacgagtc acagaccata caataccaac tggtaagaaa tgaaatatag cttcacctgg
    18961 taaccataaa aaagagtcta ctccgctcca aaacgttgaa acatctacta gagtattcca
    19021 aattggatgc ccagaagagt ctacttgcct gacaccatca acaacttttt gacctaaaaa
    19081 ttcaaatgga acggcatcta aaatattacg aaaaccaagg atcagaccac caacaataat
    19141 ggctggaata atcggtgtaa atatttcagc cagcattgtc aacacacgct gtaaagggtt
    19201 ttgatttttt tgggcagccg attttgccgc ctctttagac acaccctcaa taccagaaac
    19261 ggctacaaaa gcattataaa aaatagggac atcattacca ataatgactt gaaattgccc
    19321 agcattggta aaggtaccct tgactgatgg taactcctca attaccttta ccttagcttt
    19381 actactgtca ttcaaaacaa atctcatgcg tgtggcacaa tgtgtcactg cactaatatt
    19441 ttctttaccc ccaattgcct ctaacaaggc tttggcatca tgtttaaatt gttccatctg
    19501 tttatcctta tctatttaac ggagagtctg tgactccaag gcagaaactt aaagaagact
    19561 agcttacaat ataattgtaa acgattacat atccctatgc aaatccttag cttatctttt
    19621 taaaaaatct ataaattata aaataattag tcaataatac ccaaaacatt agaatttctc
    19681 accaacgtta ccaaaatgtt agagtcatga gtaggtcagt cacccccaat actctcttaa
    19741 taaaaaacac ctactaattg atatagtagg tgagtttttt aatctccatt gaaagatacg
    19801 atacgattct cttcaatcat atagcattgg ttacaaagtt tctctaacat agcgaaatca
    19861 tgactaataa cgatgagacc taaatttcta tcctttacaa tctccaacag gcttttccat
    19921 acactagctt gtgtaatact atccaacata gtagtcattt catctgcaat aaggtattta
    19981 gtctctggat gtaatgaacg cacaatcgaa aagcgttgta attcccctcc cgagagttca
    20041 ctagggcgac gatttagcca tttttcttga attccaaaag catctagtaa atctcgactt
    20101 gggtaataag cttcttctaa actttttttc ataggccata aaggattcat cgttttttca
    20161 ggatgttgtt gaataagttg tacaggtcta aatgctttgt taggcaaatt actgccatca
    20221 actaatactt caccagatct aggatgtaaa aaacctgcaa gcactttgga taaactagtt
    20281 tttccacaac cactttgtcc aaatatacct aaaacttgac caggtgctac ctctaaatta
    20341 atctccttaa aaagccattg atctttttta tgataaaagc caagcttttt agcctctaag
    20401 gtcatgagta actcctttca aaaattcttg ctgtgggaga gagcgccata aacttctagc
    20461 aaattctgtt tgtaactgct ctccatttcc gctaaagaaa ctagctggag ctgtttcaat
    20521 agcttttccc tctttaaaaa tagtaatacg atcagcaatt tgactagctg ctacaatatc
    20581 atgagtgata aatataacgc ttattccttt atctgcaaag gagcgtagtt ggtctaaaac
    20641 catttgcaga gcatctggat gtaatccagg ggtgggctca tccgcaataa tcaaagaaac
    20701 cttatcacta atacacgttg taaacaaaac acgtcggagc attccgccag aaagttggaa
    20761 aggatacaag tcaccatcac tttcttttaa accaaactgt tgaaacaatc cttcttgagt
    20821 agcctttgaa ttttctgaga tacctaagcg cacctgatgt ttgactttca tagatggatc
    20881 taaataatta actgattgtg gaatcaacgt aatatctttt cctcgcaact gtttaatgcg
    20941 tttagaattt agtgattgac cacgataaat catatctcct gttacagatg catttttagg
    21001 aagaatatcc ataatagcat gtgctaataa actcttcccc gaaccactag cacctataat
    21061 agctaataac tcaccttttt taatttctaa attcagcgct tgtatcggtg ttgattgaaa
    21121 tggttttaaa aatcttccgt attgagtgaa ggtgatggag aggtctttaa tgcttaataa
    21181 tgtttctgtc atatctctac ctcctaaaaa tgatcagttt gagggtaaaa gagtttcttt
    21241 aaagattctc cgatagtatc aaatgcattg acaaccaaaa taagataaag tcctggaaag
    21301 ataaccaacc accaatttcc aagagagata tgcttagctg cctctgacag aatgatacca
    21361 accgaaggtt gttcggcaga gagcccaaat cctaagaaag tcattgatgc ttcatgtagg
    21421 atgacatgtg gaaataagag gataaaacca atgaaaattt gagaagcaat caaaggcagg
    21481 atatgatgcc tcacaatata ataaggcgtt tttcccatac ttttagaaag ttggacaaat
    21541 tctttattct ttagatgata gacttcattg cggataagcc ttgctaaaga aggccaatgt
    21601 gtaacagccg ttgcaatgat gaccccttga gcacctttcc caacaacaaa agaaatgaga
    21661 atcataaaaa tcaaatgagg cataccaata aacaaatcaa ctaaccatgc tattatttta
    21721 tcaataatgc tatttcctaa acctgcaagc actccaaaaa ctgtcgccag aatgaccccc
    21781 ataagggcac ctaataagcc gacttgtaga gagaaataaa gtcctttaat cgttctgaca
    21841 aacatatccc tacctaaacc atctgtccca aaaagatggt ttaatgaagg agcaaggttc
    21901 cgtaaagctg cattggtttg caagggagta ctatggaaat aaagatttaa ggctagaata
    21961 ctaagaatga gagaaatggc gatacccagt tgccataaaa caatagttcg acgtctcaat
    22021 atcatacttt tctccttaac tgtggattga ttatgctatt taaaatatcc gcaataagat
    22081 tgcccgcaaa aacaaataat gtccctatca tcacaatagc tagaagtagc ggtgtatcac
    22141 ttttaagtcc tgcttcagtt agggtagacc ctagtcctgg atatgagaaa acttgctcag
    22201 caagaacgga tcctccaaac aattctccaa aataggaaaa atgcagtgta atagctggta
    22261 cgatagcatt tctaagacaa tgatttttaa aaatttgcca ttccgtttcc ccacgcgctc
    22321 tggcaaataa gacatattca ctagaaagta ccgacatcat tttagttcta gtatgaagag
    22381 ttacattggc aatgcctaga atacttagcg tgaaaacagg taaaataagg tgcttaatac
    22441 gatcagctaa cgtaatatct tgactcaaag tacctatcgg agaagaaata ccaatcggga
    22501 accaccccag ctggacagaa aagattaata aaaaaatgag cccaatccaa aaagttggta
    22561 ctgatatctg aaggtaagaa aaccacctta caattcgatc aagcaatttc ccttgatgga
    22621 aagctgataa agttcctaag ataaatccaa taagacccga taagacccag gagagtccca
    22681 taagtatgaa agaggcacct gcccgtgatc taataatatc aataacaggt tgccgataaa
    22741 ctagtgaagt ccctaaatgt ccctgtataa aattttttaa ccaaataaaa tactggaccg
    22801 gagctggctt atccaagcca tagtggtgag caatcgcttt gtactgaaca ggggttagtg
    22861 acgtgtcata attgacactt gccatgactg gatcaaccgg tgattgtttt agtaaaataa
    22921 aggtcaaaat tgatactcca aaaacgagtg acaaacacct aagtatcttc cagataacta
    22981 cagtggtaat atgtttcaca gataaatctc cttaattgtt aaaaaaggag tcgaggaaac
    23041 cattcacacc ataaggtgta cattgttttc tctgactcca aacttaaagt agcctacatt
    23101 aactaaatta gttacttagc agattcatcc caagtccatt cagcaatgtt agtcaataat
    23161 gaccaatcat gaccatgact atggacacct tgtttaccta cattgatacg tttatcacca
    23221 atataagtat ggttaagact caccaaccat acatttggta aatctccaag agtggaagca
    23281 cctgttttgc catcccactg agctaacttc caatatttgt tagctttatc aaggtcggga
    23341 gatgtcattg ctttgtcaag gtacttagtc acagtaggat tgttataaaa agtaatattg
    23401 gtccaacctt taccagctaa acttgggtaa tgtgattcat aaaattgctg cgcatgatga
    23461 cgtcctcccg cataaagtaa ggctgaatca tgtgacttag ttgccatttc atcccagtta
    23521 ctagccttga gtttaatagt aatacctaag gctttagctt gctccgctac ttcaacggct
    23581 aagtttgctc gtaattgatc attagtaggg tagtaaaggt caaattccgc tttaagattt
    23641 ccttttttgc ggctaccgtc tgcttgttct ttccatccag cttttgtcaa aagttgctta
    23701 gctttagcta ctttattatc tttaattgct gtttttggat tccagaatgg tgttctatca
    23761 ataattgaat aagctggttt accataacca tttaaaacag tatccagaac tttttgtcta
    23821 tttaaaccaa tagtcaaggc ttttctgatt gctggatcac tagtgacatc atttcctact
    23881 ggataaccgt ctggtgaatt tttcacaaca cctttcttca cataaggtag cgataaccca
    23941 cgaacatcat ttgaagcaat atctagaaga cgtgttcctt tgactttctt gctagcaagc
    24001 tctggcgttg cgtaaatcat gtcaacatca ccagattcta aagcagctag tgctgtattt
    24061 tcatcaagta atacccaagt ccacttttta aaataaggct ttttaccgtg ccaatatgga
    24121 tttcgaacga agattgcttg ttctccagcc ttatattctt ttaccatgta aggtcctgaa
    24181 ccgataggat tgctcttata cttatcattg taatgttttt tagggacgat tgggatttct
    24241 gtcaactgtg ctgtaaatgt cgaatgcgcc tcagtcaaat gaatatttac ctggtttttc
    24301 ccaactactt caacattctt aataaaggtc aaatcccaag cttttccatc tgctttcaac
    24361 atatcataag taaacttaac atcgtcagca gtaacaggct caccattgga gaatttaaaa
    24421 tcatcattta agtcgaacga ccacgttaag ccatccttag agattttata ttttttagca
    24481 agctctcctt ttatatctag ttcaggagaa cgtttcaata gggtactatg agtaatatta
    24541 ccttcattat ggattccata acggtccttt ggatcgaatt catgaggaag ctttgccccc
    24601 atagagacaa ccaattcatc tttgggtcgt tgttttcgtg ttcgctcctt tgtttgtgaa
    24661 ttttgatttt gacaagctac taaaaagata ctcgccagaa acaaagcaac aaaagagatg
    24721 tacttcaaat atttagtcac tattaaacct ccttttttaa ttgataaata atatttttga
    24781 caactttttt cattataaca agaaaaaagt aggagaacaa ttacttaact ggttaattag
    24841 aaaatttgtt attttatcat taataactta aagttaggta aaacacaatt ctagactcta
    24901 gctcataaac catgttaatc caatgtattt taatagacat cataaaaagg ctgaataaat
    24961 tcagccttaa ccacgctatt agttatagca aaccaatcaa atgactaaag ataggaataa
    25021 ccaaatcaac taccaaaccg ataaccacca ccgctatagc tgccatcgca ccttgcagtt
    25081 ctcccaattc caatgcaaag gctgctccga ctgtatgacc agaggtccct agacctaatc
    25141 ctgcaccaat cttactatca ttcaaatgga aaaatgacac taatttttta cctaatgcat
    25201 aaataataac tgcatttaag atacacgcca ttgctgtcac agctgtgttc cctccaattg
    25261 ctgctgttat tggaagagca attgctgttg ttgctgcttg tggcaacatt gaagcaattc
    25321 cgacttgact aagcccaacc attttagaaa tagcgacgat aagcataagg gaaacaatca
    25381 taccgattac taaactgctg agaatttcta cccaatattt tttaacaacg tcatttttct
    25441 tatagagagg aactgcaaaa gcaattgttg ctggatttaa aaaccaaaat attaaatcgc
    25501 cacctggttt ataagcttgt gtataaaatg tcttaacatc ggttcctaaa ccttttgaca
    25561 taagccaaag aatgacaata ccactaacca ttgcaaagaa aagaggctgc aaaaggaaga
    25621 aacctttact cttcttaaat aaatgttgtc ctatcgtata gagtattaaa gaaaagcaaa
    25681 taccaaagat gggtgttttt aagagttcca tattattttt cctctacttt cttactatag
    25741 tggttaggta atatcttttt aaataaatct gtgacactga gttctctgat agcaagtatt
    25801 aatcgtgcaa cgtatgctac cactactaac ataacaacag ttgagattgt aataacagca
    25861 actagttgca agccttcagc ttttaggata tcaaggttcg ctgctactga tattcctgag
    25921 gggacaaaca taaaaccaat catgcttatc atcaaagccc caaaagagtc aacccactct
    25981 accttgataa ttttagcagt tagcaatacg tacattagca ccaaaccaat aaccgtcgtt
    26041 gggattggta gtgatttcgg taataacatt gaaatcattt gtgaaactaa tagaatagct
    26101 gcatatattg acatttgaat aaacattgga gcttcttttg ttttggtttc tttactggtg
    26161 tgtttcatag tcactacccc ttccttttat tacaatgttt taacttgttt tattgatagc
    26221 tatattgtac ttcaaaataa ataaaacgtt ttcattttca gcctcagtta ccaaaaaaag
    26281 aagccgaatt gcaaattcaa gcttctaaac aacaaaggga agttcgcaaa agcactttta
    26341 catggttaaa tgctcattta ggtcttttaa atacgatcga cctactggaa atttctctcc
    26401 attagacata attagtaaca aagtatgatt aaaccaaggt tgaatttcct caatcatctc
    26461 tagattaatc actgtgtttc gatgaatctg gagaaaatat ggtgctacgg cgcgtgactt
    26521 aaaccaattg agtgttttcc gaatagtgta agttttctga actgtactaa cagttaattc
    26581 gccatcttca acactagctg caacgatatc ttgcatttta acaacaacac tgcgatctga
    26641 taatgtcaag gttaacaatt cagcactggc ttttttaggt attgcttgct ctacatctga
    26701 agcagtggtg agttggctca atttcttcac ttttgataga gccatatcaa cgcgctgttg
    26761 ttcaaacggt ttcatgatat aatcaacagc attactttca aaagctttca cagcgtagtt
    26821 atcataagct gtcgcaaaaa caacaagtgg gggatgtgca agttggctca actgattagc
    26881 caaagtaaag ccattttctt cactaagcga aatatctaaa aaaattaaat caatttgctg
    26941 tctaaataag attttttcgg cttcactgat atcctcagcc tgaaaaatct cagggttgtc
    27001 aacctcctgg ctatgttcaa ctaaaaacga taattcttgt ctggcaaaca tttcatcatc
    27061 aagaattaaa attttcatgc tcatcctccc ttattctatt aggtattcga taccaaactt
    27121 ttgtaccatt cttgtcgctc gaaaaatgaa ggcaacttac actaccatat aataaattca
    27181 gcctgttatt tagattaact agagcagtac ctgtcccctt actctctgca actgtttctt
    27241 gacctaattt atcaatgata gtatctgaga ttccttgtcc attgtcacta acagaaacac
    27301 aataataatg accatctggc tttatttgaa ccaatatatg gttgtccgtc ttacgttctt
    27361 tgaaagcatg tcgaactgca ttctctacca gtacctgtaa accaaaaggc ggtaacttca
    27421 ttttttctgg tgcactaata tcataagata actgatattt atcagggaaa cgtaattttt
    27481 caacattcat ataagcatcc acatgtgatt tttcttgctc aagcgttacc tcacgatcct
    27541 gaccaccttg caaacttgtt ctaaaaaaag tacttaactg catcagtgca taacgtgctt
    27601 tatcagaatc aatacggatt aatgcactaa ttgtgttaat ggcattaaag aagaaatgag
    27661 ggttgatttg tgcttgtaaa gcctttatct ctgccatact ggctaactta ttttgttcct
    27721 ctgttatccc cattgccagt tgtcctgaaa atatttgcgc taaaccaagg actaggtttt
    27781 cctccacctc agacattgtc ttatctcctg caaagtacat ttttaaggca cacacagttt
    27841 tatcatttat ttttagagga actacaatag cagaatttaa ctgacagttg tgatctggac
    27901 aagaaatcgc cgctttatct tgcgcaattc ttggttcgcc atcaaaaata acacttttag
    27961 ataagtctgt tttgaccggt tgtcctgcaa tatggtgatc atggccaaca ccaatatgag
    28021 ctaatacgtt tgaccgatct gttaatccca cagcatcaaa gttagtatgc ctctttataa
    28081 tttcgcaaac gctcctagca gattgcggtg tcaaaccttg tctaaggtag ggcagagtct
    28141 gtcgagtcaa ttcaagaaca tctctcgttt gaactgcgcg taactgactt tcatttgaca
    28201 aataagtttt caaaatcgca aggaaaagtg tggaacctaa actatttaaa atcatcattg
    28261 gaatgacaat cattttgaca agttcccatc ctgtaaaaat gccaacaaat agcatctgga
    28321 tactttcggc aataatacta attaaaataa cttggcttgt tgaagggtag agatggtttt
    28381 ccttaagctt atcaccaatc tttccgctaa caatgccgac tagaactgaa ctgacaatat
    28441 agaaagaacc tgaaaagctt ccttgaaaaa agcgatgaac tcctccaata aaaccaacaa
    28501 ttgatccaac cagaggtcca ccaaccaaac ttgccgttgt aataactaaa gtccttgtat
    28561 tagcaagtga gtcagaatgg gaaatcgttg ttagaaaagg gcgctcgacc aaacttcgat
    28621 ccccttttat ttcaattcct gttatattag atataataac aaacaagccg aaaatgatga
    28681 caaggactac cgtttcacgt ttagaccgct cttcaattaa ctgtctgaaa taactattat
    28741 ttaccaataa aaaggctaaa atcataataa ttcctagcct ttggaataac aacaccatca
    28801 acatatgctt atcctcctca agataccatt ttactccgga agataaatat agatatcttg
    28861 ataatctgaa ataaccttgt ctgccattga taaatcttgc ggtggataat caggattggc
    28921 aaagccaaag caatacattc ctgctgcctt agcagctaaa ctcccatttc ttgtatcttc
    28981 tataactata caaactttag ggtctacatc taaaagctct gcagctctaa gaaaaacatc
    29041 tggtgctggt ttagaagacg atacatcttc tcctgtaacc atgtattcaa agcattctgt
    29101 cacacccaac tcttttagat tacgcttaat atctaccatt ggagatgagg acgcaactgc
    29161 taagcgatag ccatgttggt gtaaccaatg aatcagttgc tgagctcctt taatgggcct
    29221 aacaccatca cgcgtcacaa tcgcttgtcg acgtctgttc atctcagcaa tatactcttt
    29281 tactgttttc ggtaagccaa actcctcttt catagcttgc cacatgaatt caaaagtagt
    29341 ccccatgtac tggtattgat acgagacgtc cgtatcaata ccctcctcac gtaacatttc
    29401 tgtcttatta tctaaaaacg tgtactcgga atcaacaata acgccgtcca tatcaaaaat
    29461 aatgactttt tccatcttaa acctgctttc tatccccttt attgtaatcg tttgcctttc
    29521 tattttcaat aaaaaaaggt tgctttctgt ggaaagcaac cagtatattt agaaatgata
    29581 ttttgagagt gattttattt aaaaggggag ctcctcttct tccaatacta aatctgacaa
    29641 atcaccagaa acattatttt cacgcatagc acgttgggca cggctttcta acaactgaaa
    29701 tgatgatgct aatacttcag tgatatagtg cgtttgacca tccttttcgt acttgcgcgt
    29761 acgcaattca ccatctattg aaattaaaga gccctttgtc ccatagctcg caagggtttc
    29821 cgctagacga ccccacataa ccacattaat aaaatctgct tcacgctcac cattacttcc
    29881 tttaaagcgt ctattaacag caacagttgc acgcgttact gacttgtcag ttggtgtttt
    29941 taccatctca ggctttgctg ttagacgccc aatcataata actttattat acattgatta
    30001 cctcctcact attattattc gaaaataagg aagaaaaatg ctaaaatata tgaaattgtt
    30061 ctgggagaat taagatgaca aatgtaacta gttataaaga aatgctggct aaaccttggg
    30121 gaaaaataca atatgaaata acttttgctc aattaagcca tatcaaaaat cagaacgttt
    30181 tagattttgg agctggtttt tgtctcaccg cacaacatct agcaaaagaa aacaatgtaa
    30241 ctgctattga acccaatcct aaactgcttt acgataatca aagtgacaat atttataaaa
    30301 tcctaggttc ctacgaagca ttaagagctt tgcctgatca aagttttgat actattattt
    30361 gtcataatgt tcttgaatat atagacaaac ataatcatcc tgcttatttt gacgaatttt
    30421 ctcgacttct aaaacctaat ggagaactat ctctcatcaa acataatata attggtaaaa
    30481 tattacaatc agttattttt agcaatgata cctccactgc gatggaactc ttaacaggag
    30541 aagcaaactt taaaagtgcc agttttgacc agggaaatat ttatacctta gaagaactaa
    30601 aacaaaatac aaatttatta gttgaacgtt atcagggtat tcgtaccttc tattcattac
    30661 aaccaaacca ttttaaaact gaaactgatt ggctaaacaa aatgcttgct atcgagctaa
    30721 gtgttgctga caaagctcct tataaagata ttgctttttt gcaacacatc acacttaaaa
    30781 agtcattgta acttcttaag tttacaacta aaaatcaact tagctgaagc taagttgatt
    30841 tttactaatt tttccaatgt ttattggcgt cgaaagactc tccaacaatt acctgatcag
    30901 ataattcaat gatacctctg acttgaggag catttggaag agctaactcg cgagcactac
    30961 ataacattcc aaagctatct tctccacgaa gttttcctgg gaaaataagg ctaccatttg
    31021 gcatcatagc tcctggaaga gctgcaactg ttttaaggcc tactgaagca ttaggtgccc
    31081 cacaaacgat ttgaaccgtc ttcccatcgt tgatttctac ttgacagatg tgtaaatggt
    31141 cactatctgg atgatcaatc atttctttaa tttgcgccac aacaaatgta ggttcaatat
    31201 tattaacaag agagtcttcg aatccttctt tcagtagttc tgcattaagg attttaataa
    31261 cttgatcaga taaagttact tgacctactc cctcaatagc aattaaactt gacacttcaa
    31321 agatattcca agcaactgtt tctttactat cttgcagata gacgcgtgct acttgtccgc
    31381 gacgatcaac atctagctta gctccttgac tatccttaac aataaccatt aaggtatcac
    31441 caacatgctc tctattataa gtaaaaatca tttattctcc tttaaatttt gctatttttt
    31501 gtcttattta tggttaattt cagcgagaaa tttagtaatt tcagcctttg ttttgcgatt
    31561 cttattaacc aaacgaccca gttcctgacc attttccaca acaacgaaac tcggaatccc
    31621 aaaaatattc cactgctgag ctaattctat atagtcatca cgattaacac gtacaaagac
    31681 aaaatctgag aaatcttttt caattgatgg cattactgga tatataaatt gacaatctgg
    31741 acaccaatct gctgtaaaaa agaaaacaac tttcttagtg ctatcaatat aagctgctat
    31801 ttcttcataa gattctggta aaatcatttg ctcacctcca cataatcaag aacaccttga
    31861 cgataaatga attcgaaaat tctgccatct tccaagacaa gaccaccact ggtcatttta
    31921 atatcagatt caaattcatt aaaataaaat acctgaatat cacccatctg actaaaaagt
    31981 tctcttaagg tctgagtgat ttgctgacgt ttataatctg ttactttttt agttagggta
    32041 taaccagcag ctgccagtaa acccacacca gcaaggccag tcccaaataa gattttttta
    32101 tttttcataa ctgttattat accatacccc ccggaattag acacaggaat tgatggaaga
    32161 aatccacata acagttatga aaaaaaacaa cttattcctt gtggtataat aggatgacat
    32221 tgtcttaaga taccattatg gttaatcatt caaacagaaa gaaaaaacat gaaagaaaaa
    32281 ttattagtac ctgctttaaa ttgtttgcta ggtctactct tcctaggtga tcactggttt
    32341 ttatttcgtc ttcaaaatgg agaacttttt gctattgtga cgctaatact gctttgcaca
    32401 gcttcatttt taatcaataa gaatcacccc aaaaatttga ctttaattgc tatggttatc
    32461 acttttttag ccgtattatc tttcctatct acctttttat tagcttaagc cgataaacat
    32521 tatctccgtt gtattcaacc cagttttcac tataacaggc tttgtcagtc ataacagtct
    32581 tctggtagta cttgatattc tttttgaaag tctttatatt gactgaaacc tgttctgttc
    32641 tttattttta attaaaaggt gctaaaatag tatttgtaga attgaggtaa ttatgtcaga
    32701 tttatttaac aaaattaaaa ccgtaactga gcttgatggg attgctggct atgaacacaa
    32761 tatccgcaac ttccttcgtc aagaaataac tcctttagtt gatcaagttg agacagacgg
    32821 acttggtgga atttttggag taaaaaatac tcatgagact aatgctccta aagtcatggt
    32881 tgctgcccat atggatgaag tcggctttat ggttagtcat attcagccag atggaacatt
    32941 tcgtgtactt gaggttggag gatggaatcc cctagtagtc agctcacaac gctttaccct
    33001 ctacacacgt tctggtgatg ccattcctgt tatatcaggc tcagttcctc ctcactttct
    33061 tcgtggacaa agcggtggaa caacattacc caaaattagt gacattgttt ttgatggagg
    33121 attcacagat aaaaatgaag ctgaaagctt tggcattgct cctggcgata tcattgttcc
    33181 taaatctgaa accattttaa ctgcaaatca aaaacatatt atgtcaaaag cttgggataa
    33241 tcgctatggt gtgcttatgg tgaccgaatt gctaaaaagc ttaaaagatc aaagtcttag
    33301 caacacactt attgctgggg ctaatgttca agaagaagtc ggacttcgtg gcgcacatgt
    33361 ttcaacaact aaattcaacc cagatatctt cttagctgtc gattgttccc cagctggaga
    33421 tatttatggg gaacaaggca aaataggaga gggaacctta atccgttttt atgatcccgg
    33481 acatatcatg cttaaagata tgagagattt cttgcttaca acagctgaag aagcaggtat
    33541 aaaataccaa tattatgctg caaaaggtgg taccgatgct ggggctgctc acctaaaaaa
    33601 tagtggtatt ccttctacaa ctatcggtgt ctgtgcacgc tacattcatt ctcatcaaac
    33661 actctacgct atggatgatt ttctacaagc acaagcttac cttcaggcca tcgttaacaa
    33721 attagaccgc tcgacggtgg atattattaa aggttattaa gatagaaagg atgggagttt
    33781 cataaggtag acacatttta gtatctcctt tcatccccat atcaacatta tgaaaattgg
    33841 aattatcggc gttggaaaaa tggccagtgc aattattcaa ggcctcaaac aaacccaaca
    33901 tgacattgtc atatccggat cttgcttaga gcgttcaaaa gaaattgcag aaagacttga
    33961 tgttacttac gccgaatctc atcagtcatt aatcaatcaa gctgatatta tcatgttagg
    34021 tattaaacct caactatttg aaaaggttct tcttccactt gatatcacaa aaccaatcat
    34081 ctcaatggca gcaggaatct ccttagcgcg tttaagccaa ttaactcgtt ctgatttacc
    34141 attgattcgt attatgccaa atattaacgc acaaatactt caaagttgca cagctatttg
    34201 ttacaacaat catgtatcag atgagttacg acaattagcc aaagaaatta cggatagttt
    34261 tggaagtagc tttgatatag cagaaatcaa ttttgatacc ttcactgctc tagcaggttc
    34321 tagtcctgcc tatatctatc tatttataga agccttagct aaagcaggtg taaaatatgg
    34381 atttccaaaa gaacaagcac tctctattgt aggacagact gtcttagctt ctagtcaaaa
    34441 cctattacaa ggacaaaata gcacctctga cttaatcgac aacatttgta gcccaggcgg
    34501 aactactatt gctggccttt tagacttaga aaaaaatggt ctgacgcata gtgttatttc
    34561 tgccattgat gctactattg agaaagcaaa aaaactttga gcaaattgct caaagttttt
    34621 ttagttattc atcattgtac tgattaacac gctataagca agagcattgt taataaaatg
    34681 aatcattata ctatattcta agtgctcagt tttataataa acaaaagata aaatagcacc
    34741 catcccagca taaataataa aactacctat atcagaaggg gaatgaataa gagcaaaagc
    34801 taaagtaccg atgacaaaac cccatttttg atgtttcgca aaaagttctt tagggataat
    34861 atagcgaaat ataatttctt ccataattgg tgcccctatt atggcaaaaa ttaacatcaa
    34921 agctagtgga agttgctttg ttaattccat aattactgtt tgatttgctg tatctttaac
    34981 cccttgcata gtcataatga ggtaaccaat aatttgtgga accctcaatg ataaccaacc
    35041 taagagaata gtaactaatg ctttccttgt caataatact ttccatctga ttatttgttt
    35101 aaccttggca taataaagga aaagagcaat aataagtaat tctaagccaa ttaacaatat
    35161 actagtaaaa ggcgtttgat agtgattctt taggagccac acagccaata taggaacaag
    35221 ttgattaata actaaaaatg ctattagcaa agctaaaatt ttgccttttt taataataaa
    35281 tgacacaaaa acctccaaaa taaattatta acttttttct atcataacat attttcaaat
    35341 cttata
//
LOCUS       DATRSG010000011        34718 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRSG010000011 DATRSG010000000
VERSION     DATRSG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34718
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.011"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..120)
                     /locus_tag="VBD89_000573"
     CDS             complement(<1..120)
                     /locus_tag="VBD89_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO7135126.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLD"
     gene            complement(241..1218)
                     /gene="dusB"
                     /locus_tag="VBD89_000574"
     CDS             complement(241..1218)
                     /gene="dusB"
                     /locus_tag="VBD89_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO7135127.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQNNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            complement(1202..2077)
                     /gene="hslO"
                     /locus_tag="VBD89_000575"
     CDS             complement(1202..2077)
                     /gene="hslO"
                     /locus_tag="VBD89_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO7135128.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            complement(2217..3473)
                     /locus_tag="VBD89_000576"
     CDS             complement(2217..3473)
                     /locus_tag="VBD89_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO7135129.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            complement(3470..4288)
                     /locus_tag="VBD89_000577"
     CDS             complement(3470..4288)
                     /locus_tag="VBD89_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135130.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKCFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            complement(4412..6664)
                     /gene="gshAB"
                     /locus_tag="VBD89_000578"
     CDS             complement(4412..6664)
                     /gene="gshAB"
                     /locus_tag="VBD89_000578"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO7135131.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKSCRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPYYQDLLESVKRQIQSPKLTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            complement(6834..7508)
                     /locus_tag="VBD89_000579"
     CDS             complement(6834..7508)
                     /locus_tag="VBD89_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7135132.1"
                     /translation="MDKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNS
                     HISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNEL
                     EGMLCINLDISAYQNIALDILDLVNLNVNKILSKSPQKISLPQQEEPVEVLSGNIQDI
                     ISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYL
                     KKIETH"
     gene            7870..8892
                     /locus_tag="VBD89_000580"
     CDS             7870..8892
                     /locus_tag="VBD89_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7135133.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGVILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
     gene            9263..10555
                     /locus_tag="VBD89_000581"
     CDS             9263..10555
                     /locus_tag="VBD89_000581"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO7135134.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(10578..10871)
                     /locus_tag="VBD89_000582"
     CDS             complement(10578..10871)
                     /locus_tag="VBD89_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135135.1"
                     /translation="MILINLIALPICFLAELSAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHNINSLLFLSIIVVLLISGKSLSLISIYLLKRKSKHQKV"
     gene            11210..11827
                     /locus_tag="VBD89_000583"
     CDS             11210..11827
                     /locus_tag="VBD89_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135136.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDESSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            11995..13434
                     /locus_tag="VBD89_000584"
     CDS             11995..13434
                     /locus_tag="VBD89_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7135137.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFHLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            13462..13740
                     /locus_tag="VBD89_000585"
     CDS             13462..13740
                     /locus_tag="VBD89_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7135138.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            13807..14292
                     /locus_tag="VBD89_000586"
     CDS             13807..14292
                     /locus_tag="VBD89_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7135139.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            14405..15070
                     /locus_tag="VBD89_000587"
     CDS             14405..15070
                     /locus_tag="VBD89_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO7135140.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            15074..15937
                     /locus_tag="VBD89_000588"
     CDS             15074..15937
                     /locus_tag="VBD89_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO7135141.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            15939..16655
                     /locus_tag="VBD89_000589"
     CDS             15939..16655
                     /locus_tag="VBD89_000589"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO7135142.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            16666..17313
                     /gene="fsa"
                     /locus_tag="VBD89_000590"
     CDS             16666..17313
                     /gene="fsa"
                     /locus_tag="VBD89_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7135143.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            17476..18459
                     /locus_tag="VBD89_000591"
     CDS             17476..18459
                     /locus_tag="VBD89_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7135144.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVRYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            18694..19713
                     /locus_tag="VBD89_000592"
     CDS             18694..19713
                     /locus_tag="VBD89_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135145.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVLKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            19732..20688
                     /locus_tag="VBD89_000593"
     CDS             19732..20688
                     /locus_tag="VBD89_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7135146.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            20786..22237
                     /locus_tag="VBD89_000594"
     CDS             20786..22237
                     /locus_tag="VBD89_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7135147.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVIIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            22259..23248
                     /locus_tag="VBD89_000595"
     CDS             22259..23248
                     /locus_tag="VBD89_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7135148.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAESFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            23314..24831
                     /locus_tag="VBD89_000596"
     CDS             23314..24831
                     /locus_tag="VBD89_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO7135149.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIETVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            24851..25612
                     /locus_tag="VBD89_000597"
     CDS             24851..25612
                     /locus_tag="VBD89_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO7135150.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            25678..27357
                     /locus_tag="VBD89_000598"
     CDS             25678..27357
                     /locus_tag="VBD89_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO7135151.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            27674..28765
                     /gene="ulaG"
                     /locus_tag="VBD89_000599"
     CDS             27674..28765
                     /gene="ulaG"
                     /locus_tag="VBD89_000599"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO7135152.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            28855..31233
                     /locus_tag="VBD89_000600"
     CDS             28855..31233
                     /locus_tag="VBD89_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO7135153.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            31610..32833
                     /locus_tag="VBD89_000601"
     CDS             31610..32833
                     /locus_tag="VBD89_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO7135154.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            32852..34579
                     /locus_tag="VBD89_000602"
     CDS             32852..34579
                     /locus_tag="VBD89_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO7135155.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT        10813 a         5779 c         6809 g        11317 t
ORIGIN      
        1 atctaaaact gggttattat caaccgcctc atagaaaaca tctgtcccta ggtgttgtcc
       61 tagagcagct gcaagggagc ttttacctgc tccaatggtt ccagctaaaa caatcaacat
      121 aattctcctt aattctgtta tactaaaata atttgttata cttttgagtt ttcattacac
      181 aatgatttat gctaaatttg tacagagata ggaaaaaaca gtcaaaggac tgtttttgat
      241 ttagcgtaaa cctgcaaaaa gttcttgaac ttctgctagt gtttctgcac gtgaaacagc
      301 tcctctaatt tttgctgcgc ctgattttcc acgtagataa tgaggtgcta accctcgaaa
      361 ttcacgaacg gcaatagttt caccttttaa attaactagt cgagtgagat gatcctcggc
      421 aacatctaac atcttctcaa atggtaggtc gggaaggatt tcgcctgttt caaagaaatg
      481 attaatttgt gtaaagatgt aaggattgct acgagcacca cgtccaacca ttatagcgtc
      541 agctccaatc tcttcaatca taaatttggc atcgtgaaca gtacgaatgt caccattggc
      601 aataaaagga atactagtaa cagctttagc gaccttgcca agtgtttcat ggtcacacgt
      661 gccagtatac atttgttcac gtgttcgacc gtgcatagca agtgcagata caccagcaga
      721 ttcggcagcg agtgcatttt ctacagcatt agatgagtca gaccatcctg tacgcatttt
      781 tactgttaat ggaatgtcaa gtacagacgt tacctcttta acaatatgat aaattttttc
      841 agggtcacgt aaccatttag ctccagcttc gtttttaaca actttgttaa ctggacatcc
      901 catattgatg tctacgatat ctgctttcgt attattttgg ataaagtcag ctgcacgttt
      961 taagccttct gcatcgccac caaatagttg aattgacata ggatgttcat tttcatcgat
     1021 atggagcata tgtaaggtct tttcgttgtt ataaagtaac cctttctcag aaatcatttc
     1081 cataacaacg agaccagcac caaattcttt agcgatagta cgaaaagcag aattagtaat
     1141 acctgccatc ggtgctagta cggtacgatg aggaatttca accttcccaa tcataaatga
     1201 actatttaat tgagtcatta attatcttct ccaaatcttc ttctgtgaaa tagtaagtct
     1261 ggttgcaaaa ttgacaagta atttcaacac ctttatcttc atccttcatg gcttgcaatt
     1321 ctttagttcc taaagtcaat agagcggctt caaaacgttc ttttgaacag tcacaataga
     1381 aggctaaaga gtcttccgat aagcgcttgt agtcgtcttc tccgtaaata gctgaaagga
     1441 gagattctat atgattttca gactctagca gacttgaaat agaaggcatc tcctgaatgc
     1501 gtttttcata gcgagaaatt tcttcgtcag aagcacctgg tagcacctga agcatgaatc
     1561 cacctgcaac tttaacctta tcctcgtcat ctaagaggac atttaaaccg acggcagaag
     1621 gtgtttgctc actttcggtt aaataataag caaaatcttc tccaatttct cctgtaatta
     1681 gtggagttgt tgaagtgtaa ggttgcccag ttccgtaatc ggtaataacg acaaagtgtc
     1741 cattccccat gaaaggtcca actaagacct ctccagtagc tgttttcttg atatcaacac
     1801 ccgtattttg gatgtaacct ttaacatttc ctttagtatc agctacagaa ataatatgac
     1861 caaaagagct atctccaata accttgacag ttactttact atttcctttt tgatttgctg
     1921 ctaaaatttg atttgcaatt aatgttctcc caagagctac agtagaactt gaaagtgtct
     1981 gatgtttttc ttgagctgtt cttacagttt ctgtacaatc aaggacatag gcccggaaag
     2041 aaccggaagt tgaaattgat tttataattt tatccataag tcatattata gcataaaagg
     2101 cttattttgc attggagata gtcaggaaaa tatagatgaa aacggtataa attaatgata
     2161 taaaaacgat actgtgtttt acagtatcgt ttttatatag tggatatgat agtaagttaa
     2221 gactcatcaa ctttttcttg ggtagattta ccttcgttga gtaaggcttc aactattttt
     2281 ttatcacgaa ttttgacaga atcattgatt gtttcagccg atttaataac agcagattct
     2341 aattgggcac gttccttacg tcctttgtct atggcagcga taataccatt attttgagcc
     2401 actaagcttt cagctaatgc agtgacagat ttaatagaaa cagtggggct ttgtgcggtc
     2461 ttctctaaca tcggaatcgc ttctttacta gtttcagcca gcatttgcaa tgctgcatta
     2521 ttagcgttga caatagcatc agcagtgaca ccggatttga cagattgttg catcatgcct
     2581 aactgagcga ttgagagttt cattgttgga atggtatttc gacgtaacat accaagtttc
     2641 tgacgcatat ctgacgatac tttgaccaag tttcgcatct gtggtgttgt tgcccatgca
     2701 acgtagagac ggctgacata ttccgtatgt tgctgttcga gggtattgat aacttcagtc
     2761 attcgggcta attggttact tttaatttga tattcggacg tttggctatc taatgctaga
     2821 atttcttgtt gtaagtggct tgcacgattg gcagcctcgg cttgactcga ttcaataaaa
     2881 gcaataactc caaccaaatt ttcaatagat ttagtattat cttcaatgag catttcagca
     2941 gagacgatat ttcttgccaa agtatcttct tgtttgacaa catttgctgc catcatatcc
     3001 attttttgct cgatgttttg tgagtcaaaa taaaattcct gtagcgaggt cttgctttgt
     3061 ttgaataatt tttgaatcaa gtttggtttt ttctctaatt ctgccggagt agcatcttta
     3121 tatttggcaa taaatccatt tagttcgcga ttagcatttt ttagtaaatc atcaacttga
     3181 ggaatttgaa tttttttctg ctcagacaag atatgattaa cagtagtatt aacgccttct
     3241 actgcggatt gtccaaaatc aaggagcgca ttttggtcac cgacaaaagt atctaccaaa
     3301 gctggtgttt tttcagagat agcagacttt tgtgctggtg ttagtttttc aaaaaaggca
     3361 atttgcccag tttggcttgt tgtctggttg gaaataattt ctgttgtttt atctgtttta
     3421 gtgatagcat tgtctgcaat ttggtcaata tcaaaattaa aggtatcgct catttattgt
     3481 tcctttcgtc ttgcatcatt cttaggctaa cgtcaaaatc tttcatatca ctttcattca
     3541 attggcgcag cgtttcatct aaatctaaat taaatttttt aattgcttgt aatgcttgtt
     3601 ctaggcgttc cttagcattg ttaaaatttt taggagattg tttgattctg atgtagccag
     3661 ccagaacatc tgtaaaacgt ttcatattgg cttgatgaat tgctgctaat tcagctttgt
     3721 tatctgcctc ttcaatcttt tttaagatag catagtgatc atcttgaata ttgtgataga
     3781 acggttttaa ttccggagca atgcgtatca tttcatcttc ctgatcagaa ataggttcta
     3841 gttctggaga ggcatgtagc tttattaatt gttcgttaat ctcattacta aatgtatttg
     3901 cttttttggt gatacgttta tagatattag gctcaatggc tttttgtagt tggtttgctt
     3961 cattacgaat taggtccaat tgaggtaata tttgatgtgc taatttgata tattccttag
     4021 aacgattgtc tttttttaaa atttccaagt gtctaatgtc ttggtcagtc tctttaatat
     4081 cggattttaa agcttcaata cgttggtttg atgtaaaaca tttaagacgc tttttatcat
     4141 aaacgtaata ggcacaataa ccaccaccaa taattaacag agtagcaatc acctcactcc
     4201 aaaagtctga aatcagggaa gcaagaacaa caataccaag taatgttatt agattatgag
     4261 aatttttgga atagcgtttt ttaaccataa taatatttaa cctaactttc tgtagttgtt
     4321 aatattttat cttaaatgaa ctagtgatga caagggataa ctttatttta accaaaaatt
     4381 ccttaagctt agcctaagga actcttttta gttataattc tgggaacagt ttagccaaaa
     4441 ttcgaggtgt aatagattga ccaggcccct cttgacagta ggtgtgcata tacatcaatg
     4501 ggttaaagtt tagttcaatg caagtagcat ttttcttatc ttttgagtat gcttgagtgg
     4561 cattaggaat aattaaatca actccacaaa cccaagcccc catagcctct gccatctcag
     4621 ctgctagttg tttatatgta ggatccattg tatttgttac atctatagag tcaccgccag
     4681 tggaaatatt agaattacgg cgaagttcta tttttgttcc ttctggaggg atactgttaa
     4741 cagtgtatcc ttgttgttcc aacattaact gttctacttc tccgagctca ataacctcta
     4801 gaggagagcg atgatcatag cctctgagag ggttttggtt tttaagttta actagctgac
     4861 taatagtatg aataccatct ccaacgacat tagctgctac cctaagcaat acagcaatac
     4921 agtccccctc aaggacgaag aaacggtatt cggtaccctc aatatattct tccacaagaa
     4981 tagcgctatc ttcggcaaaa gcaatgtcaa ttgctttttc ataagaagct aagttagctg
     5041 atgttttaaa aatagaaatg cctaaaccaa agtttgtaga ttttggctta acgacgattg
     5101 gtttatcttg gatttgtgaa aaatagttaa gtgcctcttt acggtcagta aattcatctc
     5161 caaaaggagt tgggaaatgc ttttcatcta ggattttttt tgtaacaact ttgttagcca
     5221 tagcgagcgg tacaatatag ttatcttttg aggtcatatt accattttta acgtactcaa
     5281 tatgactatt gtgccataat tttaggaatt gatcttgttc atccagtact tcgaagttga
     5341 ctcctttttg gataacgtca aatagtagca attgggtaga aagttccatc gtttcatatc
     5401 cctttaaggc gtagggagct tcccaagcat aatcgtgata aatttgtcct tgtctttgtc
     5461 caaatgtttc taaggaaagt ccttcaatca tttctaaaag ttgaccagct accgttaatt
     5521 taggtgactg aatttgcctt tttacagatt ccaataggtc ttgatagtaa ggtggtaagt
     5581 taaaatgttg gataacagat tgcatagcat ccactaagtc agaaactggt gcttgattag
     5641 gtaatttttc taatggatga ctgagtgcta taaggtcatt taatctattg gcttccttga
     5701 tatcttggtc aatatgacta ctagaatcta tccacaaaag cgccaataag aaaaggtgaa
     5761 cagtatcaac agtttcctgc gttataccga ttgggctaaa tgggttgaga tcaaaagtgc
     5821 gaaattctaa atatgtaatt cctttttcta aataattacg acaggactta ctaccacgga
     5881 gacgaacagg tgaataaaat tctttctcag caatcaattg cccacttttt acagcatttt
     5941 ctagatcgtt gacataatcc ttcaagctag tataagaaat acgaatatct ttatgattca
     6001 catagcctaa gtgactattt ctaagtgacc ggacagggtt attaagtttc tgatttaaaa
     6061 agtcttcttc agctactggg ctcgctccgt atagataagt tagtaaccaa cggtaacgta
     6121 aaaaattttg agacaatttc atgtaaagct ggttctgaaa atcaatagca ttatctgctt
     6181 gactcaattc aaagagagaa gttaataact cttgacctaa accgagatta tagtgaattc
     6241 cagagatgga ttgtattagt tttccataag ttttctccaa atatttgcga taatcatatt
     6301 caaaagcatc ctctaattgg gcaatctgta tgtcttcttc cctcactttg ggtggcatag
     6361 aaagcggcca taagtattca tcgtggttga tagatcgtcc agcgacatca cttatagctt
     6421 tcaaaaatcg aatggcttct tggctatcct ttgcaatagg tgtaatgagt tctaattgag
     6481 gttcactata atccgtttgg atataaggat gatagttacg actccctaat gttttgggat
     6541 ggggtgtttg agcaaccctt tgagtgggtt ggtgaatacg aaggctttct ctctctaagc
     6601 caaatgtagc ttgtagaatc ggtagatgag agtggcttct ttgtaacagt tgatcgataa
     6661 tcacgacaac attcctccta gtaacagatg atactagttt atcaaaaaaa taataaaaac
     6721 taagttttac ggcacagaaa actaaaataa tcttatactg gtgtcataaa aaataaaaga
     6781 ttgaactttt gagtccaatc ttattaatct ataagttaaa aagagcttta gagttaatga
     6841 gtttcaattt ttttaagata acgatagacg cttggttcgg agatgttgag aacttcagcg
     6901 acctttgaaa cggcaccttt taattggaat acaccttttt cgtgaagttt agaaacaatt
     6961 tctaccttaa cttcttgact taaatgaata ttctgattta aaagactagg atcaacaatc
     7021 tcactaataa tatcctgaat attgcctgat agaacttcta caggttcctc ctgttgaggg
     7081 agagaaattt tttgagggga cttagatagt attttgttaa catttaaatt aactaagtct
     7141 agaatatcca aagcaatatt ttggtatgca gaaatatcaa ggttgataca taacatccct
     7201 tcaagttcat tttgagcatt cttgatgaag aaagtagatc cacgaacttc tttattgaga
     7261 gggctgacaa tcgccttata gttagtaaca aaatcttttt ccttataaac tttattcttg
     7321 attaaatcaa gggcgaaagt cgttagaggt gaacttatgg ttcgtccact aatgtgacta
     7381 ttaaccaact caccaatata gatatcattt tcatctacta catgcaaaac aatttcgtaa
     7441 ttatcaccta aaacattatg gagaaacgtt attatcgtct tccaatagtc taatttttct
     7501 ttatccatat cgtagttcct ctatgctaat cttactatta tagtaatata tttatggtta
     7561 aaagacaata tcagactatt ttaaaagatt aatgtaataa aaaattatcg taataaaaaa
     7621 aagttaagaa aataaaaaat tattattgac tatggttatg cttttaggta aaatgtgatt
     7681 atattagcaa ctaaggagta atccatgaga taagcctaat aattatgtcg gacttctttg
     7741 ttgcacctta tccagttcgt agtacaatta agtttcagta ttacctaaag gcgcctttat
     7801 tgaaattgaa gcagtagtac acagacactg agaataaaaa acttaactat ttttagcggg
     7861 agtatattta tgaatatcat tattgggacc agtcttttaa tacttgtatt agcgattttc
     7921 actttattta attataaagc accctacgga acaaaagcaa tgggagcatt ggcatctgcg
     7981 gcttgtgcat ccttccttgt agaagctttt caagattctt tctttggtaa agttttggga
     8041 tttcaatttt taagtgaagt gggtggtgct aatggttcac tttctggtgt agctgcagct
     8101 atattggtta ctattgctat tggtgtaacc ccaggatacg cagttttaat tggtttatct
     8161 gtttctggaa caggtattat tcctggtttc cttgcaggat atttagtggg atttcttgtt
     8221 aagtggatgg aaagaaatat tcctggtggt ttagatctta tttcaattat tattattggt
     8281 gcaccattaa cacgtcttgt ggccaaactg ttgacgccgc ttattaatag cactttgctc
     8341 accattggag atatcttaac atcaggtgct catagcaacc ctattttaat gggggttata
     8401 cttggcggga caattgtagt agtggcgaca gcaccacttt cttctatggc attgacagct
     8461 atgctaggat taaccggaat gcctatggct ataggagcct tgtctgtctt tggttcgtca
     8521 tttatgaatg gtgtactttt ccataaatta aaacttggaa gtcgtaaaga taatatagct
     8581 tttgctgttg agcctctaac tcaagctgac gtgacttcag ctaaccctat tccaatctat
     8641 gtcactaatt ttgttggtgg tgcagcttgt ggtattttaa ttgccttgat gaaattagtt
     8701 aatgatactc ctggaacagc gacaccaatt gcaggatttg ctgtcatgtt tgcctataac
     8761 ccaatgataa aagtactaat aaccgctcta ggttgtatta tcctatcttt actagcaggc
     8821 tattttggag gcattgtttt taaagattat aaattagtta caaaagaaga gctacaggct
     8881 agagataact agttacttca tgaaaagcta atagtaaata tcattaatat agatatgata
     8941 tctgacttgt gataactgtt attgataatt taaaattcaa cgactctaat aaaaagattg
     9001 ttgaattttt ttattgttaa gaatgctggt atcagtaaat ttcaagattt ctttatcaac
     9061 tataatgtta gttttttacc gtttttttac acttctaata attatttttt tctttttttc
     9121 cgataatatt tgaggaattt ggcaaaaaaa aacatttttt gctataataa ggtgtcagac
     9181 aaactcgcag gtgaaattcc tgccatgttt ttccagaaag gattggggag tcccaaaaag
     9241 gctcactcag tgttaaataa atatgacttc agttgttgta gtaggtaccc aatggggtga
     9301 cgaaggtaaa ggtaaaatta cagatttttt atctgctgac gcagaagtaa ttgcacgtta
     9361 tcaaggtggt gataacgctg ggcatactat cgttattgat aacaaaaaat ttaaacttca
     9421 tttaattcct tcaggaattt tcttcaaaga aaaaatttct gtcattggta atggtgttgt
     9481 cgttaatccg aaatcattag ttaaagagtt agcttatctt cacggagaag gtgtaacaac
     9541 ggataacctt cgtatttcag atcgtgcgca tgttatcttg ccataccaca tcaagcttga
     9601 ccaactacaa gaagatgcta aaggtgataa taaaattgga acaaccatca aaggaattgg
     9661 gccagcttac atggacaagg ctgcgcgtgt cggtatccgt attgctgacc ttcttgatag
     9721 agaagttttt gctgagcgtt tgaaaattaa tttagctgag aaaaatcgtt tatttgaaaa
     9781 aatgtatgac agtacacctc ttgaatttga tgatattttc gaagaatatt atgaatatgg
     9841 tcaacaaatt aagcaatatg tgactgatac atccgttatc ttaaatgatg ctcttgatgc
     9901 cggtaaacgt gttctttttg aaggagcaca aggagttatg ctcgacattg atcaaggaac
     9961 atacccattt gtaacatctt ccaatccagt agcaggtggt gtcacaattg gttcgggagt
    10021 tggaccaagt aaaattaata aagtggtagg tgtatgtaaa gcttacacta gccgtgttgg
    10081 cgatggacca ttcccaacag aactttttga tgaggttggt gaccgtattc gtgagattgg
    10141 taaagagtat ggtacaacga ctggtcgtcc tcgtcgcgtt ggatggtttg attctgttgt
    10201 tatgcgtcac agccgtcgag tatcaggtat tactaacctc tctctgaatt caattgatgt
    10261 tctttcaggg cttgatacgg tgaaaatttg tgtggcttat gaccttgatg ggaaacgtat
    10321 tgactattac ccagcaagcc ttgaacagct aaaacgttgt aaaccaatct atgaagaatt
    10381 accgggctgg tctgaagata ttacagcttg ccgtagctta gatgatcttc cagaaaatgc
    10441 acgtaattac gttcgccgtg ttggcgaatt ggttggtgtt cgtatttcta ctttctcagt
    10501 agggcctggt cgtgaacaaa caaacattct tgagtctgtt tggtcaaata tttaatatta
    10561 aaaatctgac tgtacagtca gactttttga tgtttacttt ttctttttaa tagatagata
    10621 ctaataagac ttaaggactt cccagaaatc aataaaacta ctataattga taaaaaaaga
    10681 agtgaattga tattgtgaat caaagtatgt aataatacta gcgatacaac tgttagaata
    10741 ctaccagtca tcaataagcg ttccagagga tagtagccat gttgcttatg catttctaat
    10801 tttaatagta gaccagctga caattctgct aggaagcaaa taggtagagc tatcaaatta
    10861 ataagaatca tagaccaacc aaatccttga ccataaccta tatatgatag actatttttg
    10921 ggaattgttg acgtgtgaat tataaaatca atcgtaaaga taatgtctat aagaagtagt
    10981 agcgctaaca gataatgtag taagaagatt gttcttaacc gtttattaat caatatttca
    11041 tttcctccta cttatttacc aataagctta ttatatcata atgtataatc aaacattcgt
    11101 tagtactgat taaaaggttt atggaggggc ggttactatt gtttcattga attagtaact
    11161 ttaacgacga aatgttataa tgttattagt ttataaaaga ggagattgta tgcgtaaatt
    11221 tttccaagat gttattgtag aggtggctta ttatttagag attgctttgt cagttattct
    11281 ggcaacagct attattttct ttagtttagc actctttcat gatttgttta gcatgttgtc
    11341 tgttgatcaa aatgttgata agttgttcca gactttcctt gctcgcgcca tgacaatagc
    11401 agtcgggatt gagttgatca aaatgttttc aaaaccttcc cctgatactt taattgaagt
    11461 gttattgttt gccttagctc gtcaacttgt aattgaacat ggtagtgttt cagattacct
    11521 tatggggatt gcttcagttg ctattttgtt tgctgttcgt aaatatcttt tcacccattt
    11581 tgatgagtca agcaacattg ttttacgtgc taataacaaa gtcaaaaaag ctaatatttt
    11641 agctagggtt caactacctt gtcaacccaa tgaaacttta ggagacttac ttgttcgtca
    11701 tttaaatgat gaggaaaagg acgttgctat tggttcagta gtctatatta ataacgttgc
    11761 attgcgtgtt gatagtatgc acgatggtct tgtgacgcgt gtagaaatca ttaggagtct
    11821 acattaaaaa atataaaaaa tcatgtatct taggatacat gatttttttg taaattaggt
    11881 tcagcacaat tttgcacact taagtgtgta cttcttggtc ttttttaaaa cgcttacaaa
    11941 aaatataata aagatacaaa aaaacattac ttaaataaag agaggtcttt agttatggaa
    12001 aacttcctag ctccccttaa ttggttctcg caaaatatct tgcagaatcc agccttcttc
    12061 gtaggtctgt tggtactcat tgggtatctt ttattgaaga aaccacttca tgatgtcttt
    12121 gctggtttta tcaaagcgac cgttggatat ttgattttga acgtaggtgc aggaggattg
    12181 gttaatacat tccgtccaat cttagttgct cttgctaaaa aatttcattt agaagctgct
    12241 gttattgacc cttactttgg tttagcatca gcaaatgcta agttagaaac aatgggcttt
    12301 atcagtgttg caacaacagc actcttgatt ggttttggga ttaacattct cttggttgct
    12361 cttcgtaaag taactaaagt ccgtactctt tttattactg gtcatatcat ggtacaacaa
    12421 gctgcaacaa tttcagtatt tgtactgctc ttgattccac aacttcgtaa tggttttggt
    12481 gcatgggctg ttggtatcat ttgtggtcta tattgggcgg taagttctaa tatgaccgtt
    12541 gaagcaactc aacgccttac aggtggtggc ggatttgcca tcggacatca acaacaattt
    12601 gcaatttggt ttgttgataa agttgcgcca tttttcggta aaaaagaaga aaatcttgat
    12661 aacttaaaat taccaacatt cttaaatatt ttccatgata cagttgttgc atcagcaaca
    12721 ttgatgctag ttttctttgg aggaatctta gctgtccttg gtccagacat tatgtctaat
    12781 gttaaattaa tcggacctgg tgcatttgta ccaactaagc aagctttctt catgtacatc
    12841 cttcaaacat cattgacatt ctcagtttac cttttcattt tgatgcaagg tgtacgcatg
    12901 ttcgttacag aattgacaaa tgccttccaa ggtatctcaa acaaacttct tccaggatca
    12961 ttcccagcgg ttgacgttgc agcttcatat ggttttggtt cttcaaatgc agttctttca
    13021 ggatttgctt ttggtttgat tggtcaatta attacaatcg ctctacttgt tgtcttcaaa
    13081 aatccaatct tgattatcac aggattcgtt ccagtattct ttgataatgc ggcaatcgct
    13141 gtttatgcag ataaacgtgg tggctggaaa gcagcagtag ctctatcatt tatctcaggt
    13201 attattcagg tggcacttgg tgctgttgct gtaggtcttt taggacttgc aggtggttat
    13261 catggaaata tcgactttga attcccatgg ttagcatttg gttacatctt caagtattta
    13321 ggtattgctg gttacgtgat tgtatgtctc ttcttcttag ctattccaca acttcaattt
    13381 atgaaatcta aagataaaga agcatactat cgtggtgatg cgcctgcaga ataatagaaa
    13441 tttataacgg aggaaataaa aatggttaaa gttcttacag catgcggaaa cggcatggga
    13501 tcatcaatgg ttatcaaaat gaaagttgaa aatgcgcttc gtcaattagg cgttagcaac
    13561 tttgagtcag catcttgctc agtcggagaa gcaaaaggtt tagctgcgaa ctatgatatc
    13621 gtggttgctt caaaccacct tatccatgaa ttagacggac gtacaaaagg tcatttagta
    13681 ggtcttgaca accttatgga tgacaatgaa ataaaaacaa aactacaaga aattttgtaa
    13741 gttaatagac aaagggagtg agacaaaagt ttctctccca tatctttaga cttttaagga
    13801 gaaatcatga atttaaaaca agcctttatc gaaaatgact caattcgtct taaattatct
    13861 gcaagcgatt ggaaagaagc aatcaaatta tcaattgatc cattaattga aagtggtgct
    13921 gttgatgcag aatattacga tgccattatt gaatctacag aggaatttgg tccgtactac
    13981 attcttatgc cgggtatggc gatgcctcat gctcgtccag aagcaggcgt aaaacgtgat
    14041 gcattttcat taattacatt aacagagcca gtagtctttc cagatggcaa agaagtctca
    14101 gtattgttag cacttgctgc aacaagttca gctattcata cttctgttgc tatccctcaa
    14161 attattgccc tttttgaatt ggaaaattca attcagcgct tgacggaatg ccaagaagca
    14221 aaagaagtcc ttgcaatggt agaggaatca aaaaacagcc catatttaga aggtttagat
    14281 ttagaaagct aatcaataaa ctgatattaa tagaaaggat aggacttata aaaaatctct
    14341 gtgataagaa actctttgcc acaaataaag tgagtatttt tatcaggtcc ttagaataaa
    14401 caacatgaca aaacgtttac caaatttaca agttgcactt gaccactcag atttacaagg
    14461 agctatcaaa gctgccgtat cagtaggaca tgaagttgat gttattgaag ctggaactgt
    14521 ttgccttctt caagtaggta gtgaattagt tgaggtcctt cgcagtctct tccctgacaa
    14581 aataattgtt gctgatacaa aatgtgctga tgctggtggg actgttgcta aaaataatgc
    14641 tgttcgcggt gcagactgga tgacttgtat ctgttgtgct actattccaa caatggaagc
    14701 agccttaaaa gctatcaaag aagaacgcgg cgatcgtgga gaaattcaaa tcgaactata
    14761 tggtgattgg acttatgagc aagcgcaaca atggttggat gcaggtattt cacaagccat
    14821 ttatcaccaa tctcgtgatg cgcttcttgc tggcgaaaca tggggagaaa aagatcttaa
    14881 taaggttaaa aaattgattg atatgggctt ccgagtttct gtaactggtg gtttatcaac
    14941 tgatactctt caattatttg aaggtgttga tgtctttact tttattgcgg gtcgtggtat
    15001 tacagaggca gatgatccag ccgcagcagc acgtgcattt aaggatgaaa ttaaacgtat
    15061 ttgggggtaa tgtatgacac gtccaatagg aatttacgag aaggcaacac caaaacattt
    15121 taactggtta gaacgcctac aatttgctaa agaattggga tttgattttg tagaattatc
    15181 cattgatgag tcagatgaac gtttggcacg tttggagtgg tcaaaagaag aacgccttga
    15241 gttggtcaaa gctatttttg agacaggagt tcgtgttcca acaattacat ttagtggtca
    15301 tcgtcgtttc ccaatgggat ctaatgatcc tgagaaagaa gcgcgtgcga tggatatgat
    15361 gaaaaaatgt atcgtctttg ctcaagatat tggtattcgt aatattcaat tagcaggata
    15421 tgatgtgtac tatgaagaaa aatctccaga gacacgtgca cgctttatta agaatcttcg
    15481 tcaagcttgt acttgggccg aagaagcaca agttattctg tctattgaga ttatggatga
    15541 cccatttatg aattccattg agaaatattt ggctgtggaa aaagagattg attctcctta
    15601 tctctttgtc tacccagata ctggaaatgt atcagcatgg cataatgatc tttggagtga
    15661 attttataat ggacatcgct caattgcagc gcttcatatt aaagatacct atgctgttac
    15721 cgaaacgtct aaaggtcaat tccgtgatgt tccatttggt caagggtgtg ttgattggga
    15781 agaaatgttt gctgtcatta agaaaacaaa ttataatggt ccattcttga ttgaaatgtg
    15841 gtcggagaac tgtgagactg tagaggaaac acgtgcagcc attaaagagg cgcaagattt
    15901 tctttaccca ttaatggaga aagcaggagt gaaataatat ggcaaaatca ttgcaagaaa
    15961 tgcgtgaacg cgtgtgtgaa gctaataaat cactaccagt tcatggttta gttaaattta
    16021 cttggggtaa cgtttctgaa gttgaccgcg aagcaggatt gattgttatc aaaccttcag
    16081 gagttgacta tgaccaattg acacctgaaa atatggttgt cacagactta gagggaaata
    16141 tagtggaggg ggatttgaat ccatcatctg atcttccaac tcatgttcaa ctttataaag
    16201 catggccaga agtcggtggt attgttcata ctcattcaac agaagcagtt ggctgggcac
    16261 aagcgggtcg cgatattcca ttttatggaa caacacatgc agattatttt tatgggccag
    16321 taccatgtgc taggtcactt agtgaagatg aagttaacac tgcttatgaa aaagaaacag
    16381 gtagtgttat tatagaggaa tttgaacgtc ggaatttaga tccaatggct gttcctggta
    16441 ttgttgtacg taatcatgga ccatttactt gggggaaaga tccagcacaa gcagtttatc
    16501 actcagtcgt tcttgaagaa gttgccaaga tgaatcgttt cactgaacaa attaatccac
    16561 gtgttgaacc agctccaaaa tatattatgg ataaacacta tttacgtaag catggaccaa
    16621 atgcttatta tggtcaaaaa ggacaagaac attaaggaga ataatatgaa atacttttta
    16681 gatactgccg atgtttctga aattcgtcgt cttaatcgtt taggaatcgt tgatggtgtt
    16741 actaccaatc ctactattat ttcacgtgag gggcgtgact ttaaagaagt tattaacgaa
    16801 atttgccaaa tcgttgatgg tccagttagt gcagaagtga caggtttaac ttgtgatgaa
    16861 atggttaccg aagctagaga aatcgctaag tggtcaccaa atgttgttgt taaaattcca
    16921 atgactgaag aaggtttagc agcagtttca caattatcaa aagaaggtat aaagacaaat
    16981 gtgacattaa tatttactgt ggctcaaggg ctttcggcaa tgaaagctgg tgcaaccttt
    17041 attagtcctt ttgtgggtcg tcttgaagat attggaacag atgcttatgc tttgattcgt
    17101 gaccttcgtc acattattga tttttatggt ttccaatctg agattattgc tgctagcatt
    17161 cgtggattgg cgcatgtgga gggcgtcgct aaatgcggtg cgcacattgc cactattcca
    17221 gataaaacat ttgcatcact atttactcac ccgcttactg ataaagggat tgaaactttc
    17281 ttaaaagatt gggatagttt caagaagaag tagtgttacg ataaagcaaa ctcttaatta
    17341 tactagtgaa agacaaactt tgataatggg tttgtctttt ttataatctt ctccaataag
    17401 aacacttatt ttcatttttc ttattaaatg atataatacg gataaattaa gtctaattag
    17461 aataaggaaa tagagatgtt tgagaaggta accattagtg atattgcaaa tttagtaggt
    17521 gtgtcaaaag ctacagtttc ctattatctc aatgggaact ataagaaaat gtctctgcaa
    17581 acgaaagaaa aaatccgttt agctattaag gagactggtt atcaaccaag taagattgct
    17641 caaagtttag ttacaaaaaa tacccgtact attggggtcg ttattgctga tattaccaat
    17701 ccatttattt cgtctgtgat gaagggaata catgatactt gtcaacaatt tggttactcg
    17761 gttaacttta ctaactcgga taatgatatt gacattgagt tggaaaattt aaaccgttta
    17821 aatcagcaaa acgtttcagg aattatttta gattctgtag atcctaatca ttcatttata
    17881 gaaactttga gtaatgatcg tcttgtcatg gttgatagac aagctaaaga tattaaggta
    17941 gatactgtag tatccgacaa taaagaatcg actcaaatat ttcttgaaaa aatgcaagaa
    18001 actgggtatc atgacatcta ttttgtcact tatcccattg aagggatttc aacccgtgag
    18061 cttcgttacg aaggttttaa agaagtggtc agttcaaatc ctgataagtt gattattatc
    18121 actgaagatg gtagcacaca gagaattctt gatattattg aacattcaga gcaaaaacca
    18181 gggttcttaa tgatgaacgg tccaactctt ttgaatttta tgaaaaaatt aaatcaatca
    18241 acggtcagat accctgagga ttatggtctt gggagttacg aagatttaga atggatgcag
    18301 gtgcttacac caaacgtttc ctgtataaaa caggattcat atggcattgg ttgtctggct
    18361 gcccaatgtt taattgaaaa aattagtcaa ggaaacgaac caactactgc aaggttacta
    18421 gaagtaaaaa atcaaatcgt tattcgtcaa tctttttaaa aatgtttgcg agagcaagca
    18481 tttttttata aaaaagcaaa aaacactatg taaacgcttg cttttataaa aaatgatagt
    18541 ataatagaac tataaatgag ttaaccgttt aacttaagaa taggtaagct tttaacgaac
    18601 catctctaac tctcttaact caaatgtttt tatttttttg caccattagt taaacggtta
    18661 actcaagttt tatcttcacc aaaggaggat agaatgaaga ttaataaatt attgtcagac
    18721 agacttgaag cttgttattc agtttgtcgt ttgcaaaatc aagactatga ttttttatta
    18781 gtagcttcag aggttgatca agcctgcttt gcttatgatt tgaatagcga tttagcaaaa
    18841 attgttgttt gggaagatgt gggaggtacc atgagtatta ttccaattcc cggaaccatg
    18901 gattttttgg caacccagaa attttaccca gggtttaagg ccaaagattg tcaggttgtt
    18961 tatgcaagtt ttcaaggaga tcattgggac atttgtaacg catttgattt tccgtattta
    19021 catcgctttg atctcattaa aggtaaagaa aatcaacttt actttatagg ttgtacaatt
    19081 gctaacagta aagcctacac tgaggattgg agtgataaag gccgaatttt tgttgctcgt
    19141 tttaatactc aaaaccatac attggaagga ttgcaacaat tgcctcaaac tttattaaaa
    19201 aatcatggat actatgccat tcaggatgaa ggatattcat tgattacttc agtagaaggg
    19261 gtactcaaac tcacttatcc agaattttct actacaggcg actggcaatt agaacggctt
    19321 ttcgatgagg agacaagcga tgtggtgaaa gtggatatta atcaggatgg taaggatgag
    19381 tatgtgatca tccaaggttt tcatggagat cgtttacgta tcttcactga agatttcggt
    19441 cgagaattat tccattatcc tgaaaaaacc ccatttggtc acgctatttg gagtggtcgt
    19501 ttacttaatc agacttgttt cgtattcggg tggcgatcag aaaaagcaga attaaggctt
    19561 tttcactttg tagatgggca cttggtttca gaattagtag atgcaaaagc agcttctagt
    19621 aatgtcttag cttttgaaaa agatggaaaa gcttatcttt tctcagccaa taacggacgt
    19681 ggcgaagttg ctctttatca attagtaaaa taaatttaag aaggagacaa tatggacaag
    19741 aaaaaaatct tagtaacggg tattgtgcct aaagaaggtc taagaaagct tatggaccga
    19801 tttgatgtta cttattcaga agatcgccca ttttcacgtg actatgtgtt agagcattta
    19861 tctgaatatg acggatggtt actcatggga caaaaaggtg ataaagagat gattgatgca
    19921 ggtgaaaact tacaaattat ttctttaaat gcggttggtt ttgatcatgt agatactgcg
    19981 tatgctaaag aaaaaggaat tgtagtctct aattcccctc aagcagtacg agttccgaca
    20041 gcggaaatga cttttgcttt aattttagct gccagtaagc gccttgcatt ttatgatagt
    20101 attgtaagaa gtggagagtg gattgatccg agcgaacaac gttatcaagg tttgactttg
    20161 caaggttcaa cattaggtat ttacggaatg ggacgcatcg gtttgacagt cgcaaatttt
    20221 gctaaagcat ttgggatgac tgttgtttac aatgatgttt atcgacttcc tgaagataag
    20281 gaaaaagagc ttggtgtaac ctatctcgaa tttgatcagc ttattgaaac agctgatgtc
    20341 ataaccatcc acgccccagc attaccatca acaatccaca aattcaataa agatgttttt
    20401 gctaagatga aaaatcgaag ctacttaatc aacgctgcta gaggcccaat cgtttctgaa
    20461 gaagcactta ttgaagctct gaaagaaggt gaaatcgcgg gtgcgggatt agatgttttt
    20521 gagaatgaac cacaggtatc tgaaggcttg agaagtcttg ataatgttat catgagtcca
    20581 catgctggga caggtactat tgagggacgt agaactttgg ctgaagaagc agctgacaac
    20641 atcattgcct tctttgatgg caagccacaa aatattgtca ataagtaatt ggctagtgag
    20701 tgcagagcca tcttttgaaa aaatgggacc tcccattttt ttacgggtaa atgtaagcgc
    20761 ttaataattt aataaaggtg aacttatgct agaagcttta cataaattct ttgaagtttt
    20821 tggtgcagca actgttgtac ccgctatgat ttttattgtc agcctttttc taaaggtcaa
    20881 tccaaggcga gctttcttta gcgccttacg agctggtgtt ggactcactg gctttggttg
    20941 gattatatca gcttttgctc ctatggtgac taagtatatc cagcagatgg ttaaaactac
    21001 tggtctccat ttaccaattg tagatattgg ttggcaggct ggttctctca ccgccttttc
    21061 ttctgagatt ggcctctctt tctttgtttt tggcttgcta attgaattgg ggcttttctt
    21121 gcttgggatt acccgagtat ttgtcccatc taatctttgg aataactttg gctatatgat
    21181 ttggggtaca atggcctatg cagcaacagg taattttata ttgtctttcg catttatggt
    21241 ttttgtctta ctctattcat tagtgatgtc agaagtattg gcagatcgat ggtcggagta
    21301 ttacggtgtt aaaaatgcaa cgatcaactc gattcataat attgaaaccc ttattccagc
    21361 tcttattctt gatcctcttt ggaacctact tggtgtcaat aaggtaaagt tgaatccaga
    21421 atcattaaag actaagttag ggatatttgg tgagccaatg acacttggtt ttatacttgg
    21481 tgtgattatt ggtgtattag gtagtttacg taaccttgca agtattgata cttggggagg
    21541 tatacttggt tttgcagttg ctcttgcagc ggttatgact atttttcctt tgattacggg
    21601 ggtatttgct agtgcttttg ctcctcttgc tgaagcagtt gaacgcaata agaaaaaaga
    21661 aagtcaagct gagcaaggag cactggataa aaaacgttgg tttattgcag tagatgacgg
    21721 tgttggtttt ggagaaccag caactattat cgcaggtctc attttagtac caataatggt
    21781 tgttatttcc ttgattttac ctggaaatga agctttacca gtcgttgacc ttattgctat
    21841 tccttttatg attgaagcta tgatagctgt gtcaaaagga aatatcctta aagctatttt
    21901 gaatggaatc atatggtttt cactaggtct ttatgctgct agtgccctag gtccaatcta
    21961 cacagaagct gttaagcatt ttggaactgc tttaccagca ggggtaactt tgattatgag
    22021 ctttaatctc ctagctcatc ctttgacagc tctagtcttc tttgcttgga tatctgggaa
    22081 tccactctat attggtgtga cagttattat ctatcttgct ttcttgttct tgcttcgtac
    22141 tcgtcgtgaa tctatttacg cataccttaa aactatggct gataagaata gcggatatga
    22201 aggtagttgt gaaaaaatct ttattgctga agattaaaaa tctgttggag gaatactcat
    22261 gaaaaaaatt gatggacatg ctcatattgt tgatacaatt gcaggattta atggtaaagg
    22321 gcgtcttaat gcccttggaa acggttatgc tatttgggat gatggtgatt taattaagct
    22381 catcccagac gaatacggtg acaaagcatt tacagctgaa tcttttttga agtatatgga
    22441 tgctaatggt gtagataaag cagtcatttt gcaaggtcat cttaatggtt atcaaaatta
    22501 ttatacgcat ttagctatta aacgttatcc agaacgtttt acaggcgcat tctctgttga
    22561 tccatttgca gataatgcaa tgcaaattgt aaaacgtcat gttgaagtcc ttggttttcg
    22621 agctattaaa tttgaaatta gtcaaggagg gggtatccat ggataccgtg gacaaaaaac
    22681 gcctttccgt cttgatacag acccacatgt gagtcgtatt ttgacttatc tgttagacta
    22741 tccaggattt gtagttacag ttgattatgg caattgggat caaattagtc accaacctga
    22801 tgctattgct aatttagctc gcctttatac gagtctcgat tttgttgttt gtcacctctc
    22861 ttttccacat gttgagcatt caaatcgatt gcgtgctgaa cttaatatgt ggaaaaactt
    22921 caacaatatc tatactgata tttcagctat tcaagatatt gatgccccag atagttttcc
    22981 ttttccaaaa tcagaacaaa atgtacgtat tgctaaagaa gttcttgggg ctaagcgtat
    23041 catttgggga actgattctc catggtcagc gaccttcaat acttacgaag aattagctac
    23101 ttggctagag aatgttgata ttttcagtca agaggaacta gaagatgtta tgtacaataa
    23161 tgctgagcgt gtttacttta aagaagaaca tgtaaaggct aacagagcag gtatagatga
    23221 tacaaccaaa gagctcaatc tttattagaa gagttttcaa ggtacaagag tttcttgtac
    23281 ctccttttta aatcaacaat gaggagacaa attatgactt actaccttag cattgactac
    23341 ggcggtacaa ataccaaggc gcttattttt gacaaattag gacaccaaat cgctgtttcg
    23401 agttttgaaa ctttaaaaaa tgaggctcaa tctggtcatc gtcaagtaaa ccttgttaaa
    23461 acgtggaatg ctataacttc tgctattaga gaggttattc aaatctcaaa actcagccct
    23521 gagcagatta gtgcagtagc atgtattgga catgggaaag gtctttatct gctagataat
    23581 aagttggagc cacttgaaca aggaattttg tctacagata atcgtgccaa agatttggcg
    23641 caatatttcg aatctaaact tgataatatt tgggagttga ctcggcaaca tattttccct
    23701 tcacaaagtc cagttatttt acgttggctt aaagattatc agcccgaaac ctataaatca
    23761 ataggggcgg tcctttctgc aaaggacttt attcggtata agcttacagg aaaagtacag
    23821 caagaatatg gtgacgcttc aggtaatcat tggataaatt ttcaaacagg aacttatgat
    23881 ccagctattt tagatttttt tggcattaga gagatagaaa actcacttcc tgaacttata
    23941 gatagtgcag atttagttcc tgggggaatt agttctcaag cagcaaaaga gactggtctt
    24001 gtagaaggaa cccccgttgt tggagggctc tttgacatcg atgcttgtgc tcttggatca
    24061 ggtgttttag agtcagatac ttttagtgtt atttcgggaa cttggaatat taatacttat
    24121 ccaagtttaa aaccagcaaa gcaagatagt ggtcttatga cttcctattt tccagatcgt
    24181 cgttatctct tagaggcaag tagccctact tctgcaggga atcttgattt tatgttaaaa
    24241 atgctcatgc atcaagaaat tgataacgct aaatctagtg gaggttctat ctatgataat
    24301 ttagaagaat ttctcactca tactgatgcc acacatcatg gacttatttt ctttccgttt
    24361 ctttacggta gtaacacatc acaagatgct agcgcttgct tttttgggct aacaactaaa
    24421 tcgacgaaat ctcagatgat acgtgcggta tatgaaggta ttgcgtttgc acataagcag
    24481 catatcactg atttaataaa aagtaggggc ggtgtgccaa aaataattcg tttctctggc
    24541 ggagctacca actcaccagc atggatgcaa atgttttctg atatcttaaa ccttcctatt
    24601 gaaacagtag aaggcacaga attaggagga ttaggaggag ctattttagc acgtcatgct
    24661 ttagataaga tttcgttaaa ggaagcagtc caagatatgg ttcgtgtaaa agctatttat
    24721 aaacctcaat tatccgaagt aaaggggtac gaaaaaaaat atcacgccta ccaaaaatta
    24781 ttggaaacac tggatcctat ttggtcggaa ctcggtcatc tgaataagta gggatatcaa
    24841 aggagttgtt atgaaacaag aagatattat tatcaatagt attgtattca aaaaacagtt
    24901 agacaaaggt tttacacaag ttcagctcat taagttagta aaggatttag gattttcacg
    24961 atttgagata cgtcaagaat tgcttcaaga tccagataga gagctacccg ctttaaaagc
    25021 agaggctgac ttttatgata tcaatcttta ctatagtgct aatgaagacc ttatcaaagg
    25081 agggaaagtt aatccttatc ttaataaagg ccttaaagaa gcatcacagt taggagctcc
    25141 tttcattaag ctaaatgttg gacaaaccag aaacttaagt aaagaagagt tagaacctct
    25201 taaagagata ttgaaaagcc aaactattgg tataaaggtg gaaaataatc aagaccctaa
    25261 agcagctaca gtggagaatt gccaatattt tatgactcta gtgaaagaat tacaaatacc
    25321 aataagtttt gtttttgata ctgctaattg ggcctttatc aaccaagacc tataccaagc
    25381 ggtaaataac ttagcttgtg ataccaccta ccttcactgt aaaaatttta tacaggtagc
    25441 tggaaagccc cacctctcga agagcctctt cgaaggagag attaatctga ctgatttgtt
    25501 aaagagtttc tctaattgtg agtatctagc tctagaatac cccacagagt tggagatatt
    25561 aaaaagggat gttcaacgtt taatctctat cagtaatagt caaaactttt aatgaaaagg
    25621 ctttaaaaag atgttataat aagtctgtat gaattcgagt agaaaagagg tatattcttg
    25681 attattctcg ataaaaaaag ttatgacctc ctcttttacc tattgaaatt agaggaacct
    25741 gaaacagtta tggcaattgc caacgcactt aatcagtcta gacgtaaagt gtattatcac
    25801 ttagagaaga taaacgatgc actgcctagc gatgtgcctc agattgttag ttatccacgt
    25861 gtaggaatct tgctaacaga aaaacaaaaa gcagcctgtc gtcttttatt agatgaagtg
    25921 actgattata gttacgtcat gaaaagtagt gagaggttgc agctgtcttt agtatctatc
    25981 gtagtagcta aggaccgcat aacgattgat aggttgatgc aactaaacga tgtttctcga
    26041 aatactatct taaacgattt aaacgaatta agaagtgagt tagcagagaa agaatataat
    26101 ttacagttac aatcaacaaa atgtcgtggt tattttttag atggtcatcc attgtccatt
    26161 atccagtact tatataagct cttagatgat atctaccata atggaagtag tagttttata
    26221 gaccttttta atcataaact gtctcaagct tttggtgcca gcacttattt ttctaaagag
    26281 gttcttgatt attttcatca ttatctcttc atttctcaac gaagtctagg taagaaaatc
    26341 aacagtcaag atggtcagtt tatgattcag attttgcctt ttatactaat ggcttatcgt
    26401 aagatgcgat taagtcctga agtacagacc tctcttaata gtgattttag cttggtttgg
    26461 caacgtaagg aatatgagat tgctaaagag ttggctgatg agctggaaga aaattttcag
    26521 ttatcactgg atgagattga agtgggacta gtagccatgc ttatgcttag tttccgcaag
    26581 gaccgtgaca atcatttaga gagccaggat tatgatgata tgcgagctac tctaaccagt
    26641 tttttgaaag aattggaaga acgatatcac cttcactttg ttcataaaaa ggacttacta
    26701 agacaacttc ttactcactg caaggcactc ttatatcgta aacgttatgg tattttttct
    26761 gttaatcctt taacagagca tattaaagac aaatatgaag aactttttgc cataacctcg
    26821 tcttctgtaa agcttttaga gaaagcttgg caaatcaaat tgaccgatga tgatgtagca
    26881 tatctaacga ttcatttagg aggggaactt cgtaatagtc aacaatctcc taataaactt
    26941 aagttagtta ttgtatctga tgaaggaata gcgattcaga aacttctttt aaagcaatgt
    27001 caacgctact taacaaatag tgatatagaa gctgttttta caaccgaaca gtatcaaagt
    27061 gtgagtgatc ttatgcatgt agatatggtt gtctctacta gtgacgcttt agaatctcgt
    27121 tttccgatgt tagtagttca ccctgttttg acagatgatg atattattcg cttgattcgc
    27181 ttttctaaaa aaggtaactg tgcaaatagt aatcaattta ccaatgaact tgaaaaaaca
    27241 attgctcaat atgtcaagga agatagtgaa cgctacgtgc tgaaatctaa gattgagaaa
    27301 cttattcatc aagaattgct ccaagacgtc cttccccttc aaagtacagt ttgttaaaat
    27361 ataattttat ggtagcctct cagcttttct gaagacagaa aaagaagctg gggctttttt
    27421 ggtgctttta aacaatgtgc tagatattta atcgcaatga gttcctctat gacatgataa
    27481 ttaaagttga gaaagctagt acttttaatg ggagtacttt ttatttgtgc ttattttaat
    27541 taaaattatg agtctaattt ggaacagaca gcccagtttt gaacacataa atgtgtgaca
    27601 cttggtcttt ttttcaaaag tatttgaact tatactagta gatgtaagaa acaattaatc
    27661 aggaggaaat aaaatgccta acgttaaaga tattactaga gaatcatgga ttttatcaac
    27721 attcccagaa tggggtacat ggctaaacga agaaattgaa gaagaagttg ttgctgaggg
    27781 caactttgca atgtggtggt taggaaactg tggtgtttgg attaaaacac caggtggtgc
    27841 taatgttgtt atggacctat ggtcaaaccg cggtaaatca acaaaaaaag taaaagatat
    27901 ggttcgtgga caccaaatgg ctaatatggc aggtgttcgt aaactccaac caaacttacg
    27961 cgcacaacca atggttattg atccatttgc tattaatgaa ttggattact atcttgtttc
    28021 acatttccac agtgaccaca ttgatatcaa cactgcggca gccattatta ataatcctaa
    28081 cttagaccat gttaaatttg ttgggccata tgaatgtggt gaaatttgga aaaaatgggg
    28141 agttccagaa gaacgaatca tcgtgattaa accaggtgaa tcatttgagt ttaaagatat
    28201 caaagttact gctgttgaat catttgaccg tacttgcttg gtgacacttc cagtagatgg
    28261 tgcagaagaa catgatggag aacttgctgg tttagccgtg actgatgaag aaatggctcg
    28321 taaagcagtt aactatattt ttgaaacacc aggtggaaca atctatcatg gagctgactc
    28381 acatttttca aattattttg ctaaacatgg taaagactac aagattgacg tagctattaa
    28441 caactatggt gataacccag ttggtattca ggataaaatg acttcaatcg atcttcttcg
    28501 tatggcagaa aatctccgtg ccaaagtcat tattcctgta cactacgata tctggtctaa
    28561 cttcatggct tctacagacg aaatcttaca attgtggaaa atgcgtaaag aacgccttca
    28621 atatgacttc catccattta tttgggaagt tggaggtaaa tacacttatc cacaagataa
    28681 agatagaatt gaataccatc acccacgtgg ttttgatgat tgcttcgaac aagaatcaaa
    28741 tattcaattt aaagcacttc tataatggct aatgagagac tgtatgtcgc atgatatgca
    28801 gtttttcaat tttaaaatat gttactaaaa tcacaattaa gattaggaga aataatgtca
    28861 gaatttgata caaaatcata tttagaaaaa cttgatgcat ggtggagagc agctaattat
    28921 atttctgcag cacaaatgta tctaaaggat aaccctctct tgagacgaga gctggttgaa
    28981 aacgacttaa aggttcatcc aattggtcac tggggcactg tacctggaca aaattttatc
    29041 tatgctcact taaatcgcgc tattaataaa tacgatttag acatgttcta tattgaaggt
    29101 ccaggtcatg gtggtcaagt tatggtatct aattcatatt tagatggttc atatactgaa
    29161 ttaaacccaa atattgagca aacagaggat ggctttaagc agttatgtaa aatcttctct
    29221 ttcccaggtg gaattgcatc ccatgctgca ccagaaacac cagggtcaat tcatgaaggc
    29281 ggtgaacttg gttacgcact ttctcatgcg acaggtgcca tcctagacaa tccagatgtt
    29341 attgctgcga ctgttattgg tgatggtgaa ggtgaaacag gaccacttat ggctggttgg
    29401 ctatctaata cctttatcaa tccagtaaat gatggtgctg ttctaccaat cttctattta
    29461 aatggtggta aaatccataa cccaactatc tttgaacgta agacagatga agaattatct
    29521 cagttttttg aaggattagg ttggaaacct atttttgcag atgttgttga actctctgag
    29581 gatcatgcgg ctgctcatgc tttgtttgca gaaaaattag atcaagctat tcaagagatt
    29641 aaaaccattc aatcagaagc acgacaaaaa ccagcagaag aagctatcca agcaaaattc
    29701 cctgtcttag ttgcacgtat tcctaagggt tggactggtc caaaagcttg ggaaggaaca
    29761 ccaattgaag gtggcttccg cgctcaccaa gtaccaattc cagtagatgc ccaccatatg
    29821 gaacatgttg attctctttt gtcatggctt caatcatacc gtccagaaga attatttgat
    29881 gaaaatggta aaatcgttga tgagattgct gctatttcac caaaaggtga tcgtcgcatg
    29941 tccatgaatc caataaccaa tgctggtatt gttaaagcaa tggatacagc agattggaag
    30001 aaatttgctc ttgatattaa tgttccaggt caaattatgg cacaagatat gattgaattt
    30061 ggaaaatatg cagcagattt ggtagatgct aatccagata atttccgtat ttttggtcca
    30121 gatgaaacga aatcaaatcg tcttcaagaa gtgtttacac gtactagccg tcaatggctt
    30181 ggtcgtcgta aaccagatta tgacgaagct ttaagtccag ctggacgtgt tattgattcc
    30241 caattgtctg aacaccaagc agaaggtttc ttagaaggtt atgttttaac tggtcgtcac
    30301 ggcttctttg cttcatacga atcattcctt cgcgttgtag attcaatggt aacacaacat
    30361 ttcaaatggt tacgtaaatc aaaaacacac acaacatggc gtaaaaatta tccagcgctt
    30421 aacctaattg cagcttcaac tgttttccaa caagatcata atggttatac tcaccaagat
    30481 ccaggtattt taactcattt agctgaaaaa acacctgaat acattcgtga atatttacca
    30541 gcagatacta actcacttct agctgttatg gataaagcgt ttaaagctga agataaaatt
    30601 aatttaattg tgacatctaa gcaccctcgt ccacaatttt actctattgc tgaagcagaa
    30661 gagttagtgg cagaaggata taaggtgatt gactgggctt caaatgtgtc gcttaatcaa
    30721 gagccagatg ttgtctttgc tgcggcggga acagaaccta acttagaagc tttggcagct
    30781 attagcattc ttcataaggc tttcccagaa cttaagatta gatttgttaa tgtattagac
    30841 atcctaaaac ttcgtcatcc ttcacaagat gctcgcggtt tatcagatga agagtttaac
    30901 aaagtcttta ccacagataa acctgttatt tttgctttcc atggttatga agatatgatt
    30961 cgagatattt tctttagtcg tcacaatcat aatttgcata cgcatggtta ccgtgaaaat
    31021 ggtgacatca caacaccatt tgatatgcgc gtgatgtcag aattggaccg attccatctg
    31081 gcacaggatg cagctcttgc ttctttagga aataaagcac aagccttcag tgatgaaatg
    31141 aatcaaatgg ttgcttatca caaagactat attcgtgaac acggagacga tattccagaa
    31201 gtacaaaatt ggaaatggga aaatatcaag tagtcacttt tttcaggcct atacttacaa
    31261 acaaaaacac ttttagattt tttctaaggg tgttttgtta ttttatagaa gaatgaccgc
    31321 tttttcaaaa gtattaagtg gtcactttta aagttgtaat attaatacta ataagtcttt
    31381 aaataattca ataatgccat aacaataatg gtgtgggcta attaaaatat ctctaagcaa
    31441 aggtgttgac ataaagagac catacatgat attatagtta tgttctgttt cggcagttta
    31501 tttttttgag taaaaaagtg actacccttt tacgataact taacgtcact ttatagataa
    31561 gacacataac cttaaaaata agatacgaaa tttttagtag gagataagaa tgacaaatat
    31621 tttagaagtg aagaatttaa ccaaaatttt tgggaaaaag caaaaagctg ctcttgaaat
    31681 ggttaagcaa ggaaaaagta agacagaaat tcttgaaaaa acaggagcga ctgtaggtgt
    31741 ctatgatgcg agttttgaaa taaaagaagg cgaaattttc gttatcatgg gactttctgg
    31801 ttcaggaaaa tcaactttag ttcggatgtt aaaccgtttg attgacccat catcaggaaa
    31861 tatctattta gatggtaaag atattgcaaa gatgaatgta gaagatttac gtaacattcg
    31921 tcgtcatgat atcaacatgg ttttccaaaa ttttggtctt ttcccacatc gtacaatttt
    31981 agaaaataca gagtttgggt tagagatgcg tggtgtttct aaggaagaac gtacaacgtt
    32041 agctgaaaaa gctttggaca atgcaggtct attaccgttt aaagatcagt accccagtca
    32101 attatcagga ggaatgcaac agcgtgttgg tttagcacgt gcccttgcta acagccctaa
    32161 aatccttctg atggatgagg ccttctcagc tttagaccca ttaattcgtc gtgaaatgca
    32221 agatgagctt cttgatcttc aagacacgaa taaacaaact attattttta tcagccatga
    32281 tttgaatgaa gcgttacgta ttggtgatcg tattgctctg atgaaagatg gagaaatcat
    32341 gcagattggt acaggagaag agattttgac taatccggct aatgattttg ttcgtgagtt
    32401 tgttgaagac gttgatcgct caaaagtgtt aactgctcaa aatattatga taaaaccttt
    32461 aacaactgtt ttagaaattg atggcccaca agttgcttta acaagaatgc atcgtgagga
    32521 agtctcaatg ttaatggcaa cgaatcgtcg tcgccaactt ctgggatccc taactgcaga
    32581 tgctgcaatt gaagctcgta agaaaggttt gccgctttct gaagtgattg ataaagatgt
    32641 tgtaacggtc tcaaaagata cagttattac agatatcatg cctttaattt atgattcatc
    32701 tgctccgata gccgttactg atgataatga tagactccta ggggttatca ttagaggacg
    32761 tgtgatcgaa gcacttgcta acgttcaaga tgaaacagta gtagaatcgc caaaagaaac
    32821 ggtagaagct taatagagag gacaattcat tttggaaaat ttattacaac ataagttacc
    32881 ggtcgcacca tttgtagagt caacgaccaa ttggattacg aaaacttttt ctggtttatt
    32941 tgactttatc cagacaatcg gtaatgcatt gatggattgg atgacaaaaa cattactatt
    33001 cattaatcct ttacttttta ttgtactaat tacaattgct gtatttttct tagctaagaa
    33061 aaaatggcaa ttaccgacat ttactttcat tggtttgcta tttatctata accaagggct
    33121 gtgggaacag ttgattaata cttttaactt ggttttagta gcaagtttaa tttctattat
    33181 tattggtgta ccactaggta tttggatggc aaaaagtgat aaagttaaac aagttgttaa
    33241 tccaattctt gactttatgc aaactatgcc ggcattcgtt taccttattc ctgcagtagc
    33301 cttctttggt atcggtatgg taccaggtgt ttttgcctct gttgtatttg ctctgcctcc
    33361 aacagtgcgc tttactaact tggctattcg tgaaatccca cttgaattaa tagaagcatc
    33421 tgattcattt ggttcaacag ctaagcaaaa attattcaaa gttgaactac cacttgctaa
    33481 gaacactatt atggcaggaa tcaatcaaac tatgatgttg gctctttcta tggtggttac
    33541 aggatctatg atcggtgcac ctggacttgg tcgtgaggtg ttatcggcgc ttcaacatgc
    33601 tgatattggt acaggatttg ttagtggtct atcgctagtt attttagcaa tcgtgttaga
    33661 ccgtgttagt caattcttta attccaaacc gggtgagaag caagctaaaa catcaaaagt
    33721 taaaaaatgg gtaggtttag gggccctggc tctcttcatt ctagcagctc ttggtcgcat
    33781 tgttgtcaat atgacttcag gaaatgaagc caaaggtcaa aaagtgaaga tagcttatgt
    33841 ccaatgggat tcagaagttg cttcaaccaa cgttattgct gaagttttaa aatctaaagg
    33901 atatgatgtt gaattgacac cacttgacaa tgcggtcatg tggcaaacag tcgccaatgg
    33961 gaatgctgat tttacaacaa gtgcttggtt accaaagacc cacgggcaat attttaataa
    34021 atacaaaaat agtttagatg acttaggtcc tcacgttgag aacgtcaaaa taggtttagt
    34081 tgttcctaaa tacatgaacg taaatagtat tgaagaatta tcaaatcaag cagataaaca
    34141 aattacaggt atcgaaccag gtgcaggtat tatgaagtct gctaaacaat cccttaaaga
    34201 ttatccaaac ctttcaagtt ggaagttgtt atcagcatca acaggagcta tgacaacaac
    34261 cttagggaag gctatcaaaa acaaagatca agtagtcatt acaggttggt cacctcactg
    34321 gatgtttgct aagtatgatt taaaatacct taaagatcct aagaaatcat ttggtggtga
    34381 agaacatatc aatacaatag cacgcaagaa tttgaaaaaa gatatgccaa aagtatataa
    34441 aattatcgat aagttcaagt ggactaaaga agatatggaa tctatcatgt tagatatgga
    34501 taaaggtatg gagcctgcta aagcagctca aaaatggatt aaaaaccaca aaaaagaagt
    34561 ttcagagtgg actaaatagt atagaaaaaa acacgtctct cttagagcgt gttttttaat
    34621 gtgacttaag tcgttttagc tatttaatat aagttttata ttattttaat acatcaaaag
    34681 tgagagaatg aaagaaatgg caggttataa agtattac
//
LOCUS       DATRSG010000012        31817 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRSG010000012 DATRSG010000000
VERSION     DATRSG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31817
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.012"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..515)
                     /locus_tag="VBD89_000603"
     CDS             complement(<1..515)
                     /locus_tag="VBD89_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135156.1"
                     /translation="MLNSDFSISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHLGQLDTSLLLSLLPQQFITKYDYEFVYK
                     LSKVLAQYTSR"
     gene            1536..2954
                     /locus_tag="VBD89_000604"
     CDS             1536..2954
                     /locus_tag="VBD89_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEO7135157.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHSWHVACFSRQEASIIGQ
                     GDVLFLIGLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKG
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLKISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFFNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVD"
     gene            3225..4184
                     /locus_tag="VBD89_000605"
     CDS             3225..4184
                     /locus_tag="VBD89_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7135158.1"
                     /translation="MLKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFTLAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEGLYRSWGK"
     gene            4345..5247
                     /locus_tag="VBD89_000606"
     CDS             4345..5247
                     /locus_tag="VBD89_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7135159.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNIILVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            5407..6471
                     /locus_tag="VBD89_000607"
     CDS             5407..6471
                     /locus_tag="VBD89_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO7135160.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIATTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            6586..7578
                     /locus_tag="VBD89_000608"
     CDS             6586..7578
                     /locus_tag="VBD89_000608"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO7135161.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVKDTFENIL"
     gene            complement(7623..8072)
                     /locus_tag="VBD89_000609"
     CDS             complement(7623..8072)
                     /locus_tag="VBD89_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO7135162.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            8038..8742
                     /gene="rsmD"
                     /locus_tag="VBD89_000610"
     CDS             8038..8742
                     /gene="rsmD"
                     /locus_tag="VBD89_000610"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO7135163.1"
                     /translation="MAIKVNNNFLIIMGTSFLLLLAYHIKKEGKEILSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKIEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            8754..9044
                     /locus_tag="VBD89_000611"
     CDS             8754..9044
                     /locus_tag="VBD89_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135164.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            9041..9526
                     /gene="coaD"
                     /locus_tag="VBD89_000612"
     CDS             9041..9526
                     /gene="coaD"
                     /locus_tag="VBD89_000612"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO7135165.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFHNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADGIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVFREVEKMSE
                     E"
     gene            9516..10589
                     /locus_tag="VBD89_000613"
     CDS             9516..10589
                     /locus_tag="VBD89_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO7135166.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            10664..11998
                     /locus_tag="VBD89_000614"
     CDS             10664..11998
                     /locus_tag="VBD89_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO7135167.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHNNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANVQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNIIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWTIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELITGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTYDTKQLTGYPSDSDWAKEIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFEKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGEVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            12067..12645
                     /locus_tag="VBD89_000615"
     CDS             12067..12645
                     /locus_tag="VBD89_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO7135168.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            12696..13802
                     /gene="rlmN"
                     /locus_tag="VBD89_000616"
     CDS             12696..13802
                     /gene="rlmN"
                     /locus_tag="VBD89_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO7135169.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQYYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGR"
     gene            13802..14317
                     /locus_tag="VBD89_000617"
     CDS             13802..14317
                     /locus_tag="VBD89_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO7135170.1"
                     /translation="MLKRLFTEDGELTKISRRFVWMLVVIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWSYR
                     NIKGWLLTIRK"
     gene            14513..16258
                     /locus_tag="VBD89_000618"
     CDS             14513..16258
                     /locus_tag="VBD89_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7135171.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEINQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKEATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIVENLKTNRQGKSTIISAHRLSAVVHADLILVMRDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            16248..17987
                     /locus_tag="VBD89_000619"
     CDS             16248..17987
                     /locus_tag="VBD89_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135172.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            18115..18681
                     /locus_tag="VBD89_000620"
     CDS             18115..18681
                     /locus_tag="VBD89_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO7135173.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(18749..19287)
                     /locus_tag="VBD89_000621"
                     /pseudo
     CDS             complement(18749..19287)
                     /locus_tag="VBD89_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            complement(19288..20280)
                     /gene="bioB"
                     /locus_tag="VBD89_000622"
     CDS             complement(19288..20280)
                     /gene="bioB"
                     /locus_tag="VBD89_000622"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO7135174.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            20405..20920
                     /locus_tag="VBD89_000623"
     CDS             20405..20920
                     /locus_tag="VBD89_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135175.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRNGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            20910..22025
                     /locus_tag="VBD89_000624"
     CDS             20910..22025
                     /locus_tag="VBD89_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO7135176.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKEAFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            22018..23247
                     /locus_tag="VBD89_000625"
     CDS             22018..23247
                     /locus_tag="VBD89_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO7135177.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGDDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            23360..23992
                     /gene="nth"
                     /locus_tag="VBD89_000626"
     CDS             23360..23992
                     /gene="nth"
                     /locus_tag="VBD89_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO7135178.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(23993..24388)
                     /locus_tag="VBD89_000627"
     CDS             complement(23993..24388)
                     /locus_tag="VBD89_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO7135179.1"
                     /translation="MIMSTIYFISLCMSFILSYYDIKYQEYPIFLWILFTISTIILTP
                     ITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLPLHQMLFIIQIGAWLGIIYCL
                     VMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
     gene            24543..24752
                     /locus_tag="VBD89_000628"
     CDS             24543..24752
                     /locus_tag="VBD89_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO7135180.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            24749..25717
                     /locus_tag="VBD89_000629"
     CDS             24749..25717
                     /locus_tag="VBD89_000629"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO7135181.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            25729..26109
                     /locus_tag="VBD89_000630"
     CDS             25729..26109
                     /locus_tag="VBD89_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7135182.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            26341..28182
                     /gene="typA"
                     /locus_tag="VBD89_000631"
     CDS             26341..28182
                     /gene="typA"
                     /locus_tag="VBD89_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO7135183.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGVLVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            28227..28472
                     /locus_tag="VBD89_000632"
     CDS             28227..28472
                     /locus_tag="VBD89_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO7135184.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            28602..29957
                     /locus_tag="VBD89_000633"
     CDS             28602..29957
                     /locus_tag="VBD89_000633"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO7135185.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            29960..31036
                     /locus_tag="VBD89_000634"
     CDS             29960..31036
                     /locus_tag="VBD89_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO7135186.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            31040..>31817
                     /locus_tag="VBD89_000635"
     CDS             31040..>31817
                     /locus_tag="VBD89_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO7135187.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVI"
BASE COUNT        10441 a         4892 c         6306 g        10178 t
ORIGIN      
        1 tttcttgatg tatattgagc caaaactttg cttaatttat aaacaaactc ataatcatat
       61 tttgtgataa attgttgagg caatagtgat aaaagtaggg aagtatctaa ttgtcccaga
      121 tgactttctt taggtagttt agataattcc tccaaatcat tattaatatc atcagaaagt
      181 atttcacaac cagcaataaa tgaaagagca ggagtaagtc ccatagtctg gtaaatcttt
      241 tcaggccaaa gtttattttc ctttgcttcc tcaaataatt tatctataaa atcacattct
      301 ttctctttag ggaatctctc caaaaataat aagtcaagta tgatatcctt agatttcaaa
      361 ggaattgccc caccattttt ttcaaatgat tcccaatctg atttagacat attggatatt
      421 tctgccaaat cagttgacga cacgccaact tctaacctac gatggtgaat gtagttagca
      481 agtgacttac taatactaaa atcagaattt agcattccgt tctccttttg attcctattc
      541 attatgatta tcacatataa aaaatttttt tcaagtaaaa acacatgcat cttttgattt
      601 agcacatcat gattttctag agatgttgta atgattcata atattagtag tattttctat
      661 tttctattat ctatcaattt gggtttgttt agtagtttta acattcctta aaaacttaat
      721 tttataacga gcaaacaaaa atatatgcta atctacttct ttttatgcgt aaagatatat
      781 tttaaaatga caatcagatt ataaaggaac ataatgctaa aaatgaatag acatagaaag
      841 tgaagataca tgaggacttt ataaatagca ggaaagtaga tactagttag atatagtagt
      901 gcaagataga tgagaaaaaa taacgaatac tttaacttgg tcataacaat tccattctaa
      961 tggtagtaag catgcataaa atgtaggttt tatcatacta cttgaatcca gcaactagat
     1021 agagggggtt acatacgata agacagtagt attttaaacc gatcggttga cttattgata
     1081 ctgtttaggg aacaagtgtt ttgttgtcaa tgttaaattt aatttagcat tttgatggta
     1141 tcaccgcaag aatgaaagga aaagttgtat gtgctgaaaa aactaatgct tggcgattta
     1201 gaacacacgt ttactagtcg agatgacaaa aaataagtac caaatgtaaa ggtcgatatt
     1261 atcagcattg tcagcgttag gtgttataaa aatttaacat cttccaatta agctaggttt
     1321 aagtttccta agttgaacta cgcctagccg aaaaataatt tcttttttac agaactccta
     1381 acaaggattt tgaattgtca agattcttgt ttttttggaa agtcatttag aggctttttg
     1441 ggggaagtat taactattgt atccgtttac aattcctttc aaaaatgtta gtatatcatt
     1501 gagggatatg agttatggat aagggagaga ggatgatgat gactaaggaa acgttggcac
     1561 attatcaaaa gaaaatagaa caagaatctg aaaaaaagca gtctttagat gagcactcat
     1621 ggcatgtggc ctgttttagt cgacaggaag catctataat tggtcaagga gatgtgcttt
     1681 ttttaattgg tctataccac gatctaggca aagctgatcg agcctttcaa gataaattat
     1741 taaataatcc aaatcgacat gttgatcatt cttatgcagg ggcaaaatac ttatgctcta
     1801 ttattgggcc tcatctaaaa agtcgagggg ttgataaagg tgagagaatg acattcaatg
     1861 aaatggtggg ttatgtcatc tcggctcatc atgggatgta tgatttttgc tactgttctg
     1921 acgatgctga atattatagc tttaataaat ttaaaaaccg tatcaacaga gacttagatg
     1981 attatcacta tcacgaagat attaaagggt atgctataaa attagaagaa aaattgtgtg
     2041 attacggcta caaagattta agggagctta ttgataaagc ttttgataat taccaacaag
     2101 ctatgtcttc cttaaactgg caagataata gtgagtggga ttattatcag tcttgtatgg
     2161 tgagacttta cttgtcgctc ttaaaaaacg ctgatatttt ggacacagta aatgcctatg
     2221 gtcttaaaat aagtcctatg gataaaacag agcgatcctc tctaaaacat tcttatttag
     2281 cagccattga acaaaaatat gctagctttg gacgaccaaa caatcagttg aatactattc
     2341 ggacagaaat tgctgagcgt gttaaagaaa gaggtaaacg ggattccaaa gggatctatc
     2401 gcttagattt accgacagga gctggcaaga ctaatcttag tatgcgctat gcgtttcacc
     2461 aattagttca tcaagacaaa tcaagatttt tttacataac gccctttctt tcggttcttg
     2521 agcaaaatgc ttccgaaatt agaaaagtta cgggtgacct tggcgttcta gaacaccatt
     2581 ccaatatggt taaacaggct aatgaagatg atgacaagga cagtttattg tcagcttatc
     2641 ttattgatag ctgggatagt caagtagtct tgacttctat ggttcaattt ttccaaacgc
     2701 tttttaaaac aaaatcggct aatttgagac gtttttcaag tttgattaat agtgttgtga
     2761 ttctagatga agttcagtca ctgcctattg aagtcaccac tttgtttaat ttaacgatga
     2821 atttttttaa taaagttatg gatacaacca tcgttctttg tacagcaaca caacctgctt
     2881 atgattcttc agagattgac catcgtatct gttatggagg gaatttggga gaattagctg
     2941 aaatagtgga ttgaaataag tttatacgtt gcttcatcac caagttttac gtcgcaccca
     3001 ttgcgggtgc gtggattgaa atataagttc agcaacttca cgagaaaatg ttcctcgtcg
     3061 caccctttgc gggtgtgtag tttcaactat tttaaatctt tcaaattatg ctattaaaat
     3121 tgtctaactt ttaacagaga tgcgattgat ttgtattaat ccctaaaaag ataaattgct
     3181 agttcttatg ctataataac tctatcaatg tcaaaggagt ttttatgtta aaggtaagat
     3241 atggtgttgt gtcaacggca aaggtggcgc ctcgttttat tgaaggagtc cgcttagcag
     3301 gaaatggtga agttgtggct gtatcgagtc gaactcttga gtctgcgcaa gcttttgcca
     3361 acaaatatca tctcccaaag gcttacgata agttagagga tatgttagca gatgagtcga
     3421 ttgatgtgat ttatgtggca acaattaatc aagaccatta taaagttgct aaagcagctc
     3481 tgttagcggg aaaacatgtg ttggtagaaa agcctttcac attgacttac gaccaagcaa
     3541 atgaattatt cactttggca gaaagttgta atctgttttt gatggaagca caaaaatcgg
     3601 tatttattcc gatgacacaa gtgattaaaa aattacttgc ctctggtgaa ataggagaag
     3661 tgatctccat ctcatctaca acagcctacc caaacattga ccatgtaaca tggtttagag
     3721 aacttgagtt aggtggagga acagtgcatt ttatggcgcc ctatgccctg tcttatttac
     3781 aatacctatt tgatgcgact ataactcatg ctagtgggac tgcgactttt ccaaaaggtc
     3841 aaagcgatag tcaatccaaa ctactcttgc aattatcaaa tggggtcctc gtagatatct
     3901 tcttgacaac acgtttaaat ttaccgcatg aaatgattat ttatggaact gaaggtcgtc
     3961 taatcattcc acatttttgg aaaaccactc atgcaaaatt ggtgagaaat gatactagtg
     4021 cgcgaacgat tcaagttgac atggttagtg attttgagaa agaagcttat catgttagtc
     4081 aaatgatttt agaagggcaa agggtaagtc atatcatgac gcctcagttg acgctgtcag
     4141 gtgttaagat tatagaaggc ttataccgtt catgggggaa atgatgctgg ctatctagca
     4201 tattaataag gagtactaga tgttatttta gtagtccttt tttgttttgc tctcataagg
     4261 ttaattagat ttaaaagggt tttctacttg ttttttctcg atttttgttt cataatgtag
     4321 acaagtttag agtgaggtgc ttctatgttt tcgagcgatt ttggatttga gataattaat
     4381 gttgacgatc tatccttaca agaggagaaa tttttacctt cagaattgct ggcttatgca
     4441 cgtgatgaaa acgagtcttc tttcgtacgt gatattgagg ggcatttgac acttgtttac
     4501 cagcttttgg atacgcaagg tcacgttgat gatgttcgtc atgtgccgag agtgatacca
     4561 gtgactctat ttttgaaaga agatggcttg tttgtattag ctaatcataa aaatattatc
     4621 cttgtaaaaa aagcacttaa tcgcgttgaa aaggtagatt cgccgaaaca cctcttatta
     4681 tcattagtga ctgctttctc aaagcaatat tttgatgttt tagatactat ttctgaagaa
     4741 agagataaac taatcaatga tttgcgtaaa agaccaaaca aatcgaactt ggcaaggctg
     4801 gcaaatttac aaagtggaac agtacactta atgatgggaa ctaagcaaaa ttttgaaatg
     4861 ctgaccgatt tacaaaacat tgagcaagat aaagagaata ctagaaatga aaagatgcaa
     4921 cttcaagatg ctattattga agcaagacaa ttatctaaca tgtgttcctt aaattcacag
     4981 gttttccaag aactttcttc ttcttataat aatgtcctca gcaataacct taacgacaat
     5041 gtaactacct taacaattat ttccattggt atttccatta ttgctatggt taccagcttt
     5101 tatggaatga atgttaagtt gccttttgat agtgttgatg ctgtctgggt attgattatt
     5161 ttaattacga ctatcattac tattatgctc tctattgtta tgtacattta tgtccataaa
     5221 gaggataatc atcccaaaca taaatagaga attaataaga gggtgctatc aattagttta
     5281 gttttaagca tggcaacatg ctttttttgt acaaagaaac tagaattcat ctttgacttt
     5341 gagcttgctt tctaactctt aaaacgacat aatagaggat aatgtacagt gaatagagag
     5401 gttactatgt caatagaatc agattttttc cggaaaaaac gttttatttt ttcatcgctt
     5461 gaggagtttg gatttatcaa atctgaccaa gagtatatct attgtcaaac atttatggat
     5521 aatgacttta aagctattat cacgatttca ttggatggaa agatagctgg taaggttatt
     5581 gattcggcac ttgaagaaga atatttaccg cttcgagcag caaattataa tggttcgttt
     5641 gttggcgaag tacgatcggc ttatatggct atattaggtg atatttcaga ttcttgttgt
     5701 aaggatttat tatttaccaa agatcaaagt aatcgtttag cggagaagat agctacaact
     5761 tttgaagatt cagttgatta tccatttgcg aagcacccac aatatgcttc ttatcgtgta
     5821 tcaggtaaat ggtatgctct tctttttcca ttgaagatgg ggaagctaga aaatgttcca
     5881 gctcaactgt cagaagaaga ggttgaagtt ttgaatatta aagtcaatcc tcaagatatg
     5941 gaaatactct tacaaaaaga aggtatttat ccttcttatc atatgtctaa aaagacttgg
     6001 gtatctatcg ttttagacaa tactttgtct gatatagaaa tttttaaatt ggtatcagat
     6061 agtcgtaagc ttgtttctcc caataaaaaa tcaaattctg agccagagtt ttggattatt
     6121 cctgctaatc ctaaatttta cgatattgat aaggagtttg cagagcatca attgattgat
     6181 tggactcaaa aaggaaagat tgcggtggga gattatgtgt ttatttatat tacagcacct
     6241 attcgtgctt tgcgctatgt ctgtcaagtg atagaacgtg accttaaaac aagtacttac
     6301 ttaggagcaa gagatgtcaa agcttatatg cgactcaaac tgataaggca atttgaagat
     6361 tctacctatc ctgtagcatt tttagcagaa cacggcgtta agactgtgag aggaccacgg
     6421 cgaatgacca aggaattgcg acaagtcatg gcaaaaacaa ttctagagta atgactttca
     6481 gaaaaaaact tgtaaaaaag tcagaataat tctacaattc ctcttccatt ttgttacttg
     6541 aaggagtata atagctctaa cattttataa tttaaggagg aacatatgaa gaaaagcttt
     6601 attcatcagc aacaggagat ttcttttgtc aaaaatactt ttacacaata tttgattgat
     6661 aagttagaaa tcgtcgaagt gcaaggacct attcttagtc aggtcgggga tggtatgcaa
     6721 gataatttat ctggtattga acatcctgta tcggtgaaag tccttaatat acctgaagct
     6781 gaatttgaag tcgttcactc attggctaaa tggaaacgtc atactttggc aagatttggg
     6841 tttaatgagg gagaaggtct tttcgttcat atgaaagctc ttcgtccaga tgaagattct
     6901 cttgatccaa cacactcagt ttacgtagat cagtgggact gggagaaggt cattcctgat
     6961 ggtagacgta atttggacta cctaaaagaa actgtggaaa aaatctacaa agctatccgc
     7021 ctaacagagc ttgctgtgga agctcgtttt gatattgaat ccattttacc aaagcgtatt
     7081 acctttattc atacagaaga attggtagaa aaatacccag acttaagccc taaagagcgt
     7141 gaaaacgcca tcgctaagga gtatggcgca gtctttttga ttggtattgg tggagagtta
     7201 gcagatggta aaccacatga tggtcgtgca ccggactatg atgactggac aacaccatca
     7261 gaaaatggtt ttaaaggtct caatggtgat attttagtat ggaacgaaca gcttggaact
     7321 gcctttgaac tatcatcaat gggaattcga gttgatgaag atgctcttaa acgtcaagtt
     7381 gttctcaccg gtgatgaaga tcgtttagag tttgagtggc ataagacgct attacgtggt
     7441 ttcttccctc tgactattgg tgggggaatt ggtcaatcac gattagctat gttcttgctt
     7501 cgtaaaaaac atattggaga agtacagtca agtgtctggc caaaagaagt caaagatacc
     7561 ttcgaaaata tattataaaa aatcagccaa gcagttttgt ttggctgatt ttaacaataa
     7621 gtttattttt ggcttaagaa agtttggatt tgttgaggtg tagcttggct tccttgaaca
     7681 agatatttag tcatttttcc tcgttgataa tgtgctaaat ttggaacagt ggtaatatga
     7741 tgctttttga aaaaactctt caatcgtttc cctttgtatt tttgactatc aaggtagtaa
     7801 atttttgaat gaagtgtggc ttgagatttt aataattttg gtaagaaagc tctacaatat
     7861 tggcaagttg gacgtcctat aaaaatagtc gcttcagatt gttgactttt gcttactaag
     7921 gccttatctg tgatggcgat taagtcacta tttgttatca tgtgttggta ttgttgttga
     7981 gaaatagtca tggtgtcagc gctacttaag tttgatttta gcgttgtaag gcataatatg
     8041 gctattaaag ttaataataa ttttttaatc attatgggaa cctcgtttct cttactgcta
     8101 gcttatcata tcaaaaaaga aggaaaagag atactatctt ctctgtttgt tataagcaat
     8161 ttttttgata actctacctt attttttgtt ataatagtgg ttatgagagt agtagcagga
     8221 acatttggag ggcgtccttt aaaaacgctt gatggaaaaa caactcgtcc aacaactgat
     8281 aaggttaaag gcgctatttt taatatgatt ggtccttttt ttgaaggtgg tcgtgtttta
     8341 gacctttttt ctggcagtgg tagcttagct attgaggcga tctcaagggg aatggaccaa
     8401 gctgtcctag ttgaaaaaga taggcgtgcg caggtcgtta ttcaagaaaa tattgcaatg
     8461 actaagagtc cggagcaatt tcaattatta aaaatagaag ccaaccgtgc tttagaacaa
     8521 ttaacgggac aatttgattt ggtcttgttg gatccgccat atgctaagga agaaattgta
     8581 aagcaaatcc aaatcatgga tagcaagggt ttattaggcg atgatatcat gattgtttgt
     8641 gaaacggaca aatcagttga tttacctgaa gaaattgctt cttttgggat atggaaacag
     8701 aaaatttatg gcatttctaa agtaacggtc tacgttcgtt aggaggcttt agtatggatt
     8761 acaagttagt gatgattatt ctcatcttag tcatattgat ttatttgttg ttgcgccttc
     8821 actatttaga agagttgcta aaacattatc acttcaaaaa tgatgaagga gtttgttcat
     8881 tatcaagaac aaaaaatcca ttgtatctat taatggcaat gattactttt ttgccaaatc
     8941 ttatcaattg gatagctaac ggttttgagg caatagacat tgttcgcctg attcctgtgg
     9001 tagtcttttt atggttgtat tattgtggag atgacgacga atgacaaaga aagctttatt
     9061 tacaggttca tttgatcctg ttactaatgg acatttagat attattgagc gcgccagcta
     9121 tctatttgat catgtttaca ttgggttatt tcataacctt gagaagcaag gttacttttc
     9181 gattgagtgc cgtaaaaaaa tgttagaaga agctatccgt cagtttaaga atgtttcagt
     9241 tttagtggcg caagatagac tagctgttga cttagcaagg gaagtgggag caaaatattt
     9301 tgttcgaggt ctgcgtaata gccaggattt tgattatgaa gccaatttgg aattttttaa
     9361 taaacagttg gcagatggta ttgagacagt ttatttatca acgtcgccat ctttgagtcc
     9421 gatatcatct agtcgcatcc gtgaattgat acattttaag gcttctgtga agccttttgt
     9481 tccaaagagt gtttttagag aagtggagaa aatgagtgaa gaataaagat ccaaaaagaa
     9541 aacataaaag tttgctaggt ctgcttaaat ggtggattat aggttttgct tttttattgt
     9601 tagtattagc aagccttgtg gtgaggctac cttattattt ggaaatgcca ggtggagctt
     9661 atgatatccg ttcggtattg aaagtgaata aaaaggctga taaagctaag ggttcgtaca
     9721 attttgtagc ggtatctgtc agtcaagcaa cgccagctca ggttctttat gcttggttaa
     9781 ctccttttac agaattatct agtaaagagg agacaacagg tggctttagt aatgacgatt
     9841 atctcagaat taatcaattc tacatggaga cttctcaaaa tgagtccatc tatcaagctt
     9901 taaagttagc taataagcaa gtatctctta cttataaagg agtttatgtg cttaacttag
     9961 ctaaaaattc gacatttaaa gatagattac atttagctga tacagtcaca ggagttaatg
    10021 gcaaaagttt caaaaattct tctcaactta tcaaatatgt tgcagctctt catctaggtg
    10081 ataaagtaaa ggtacaatac acaagtcaag gtaaaaagaa agaatcagtt ggtaaagtta
    10141 ttaaactatc aaacggtaaa aatggtattg gtatcggttt gacagaccat acagaagtat
    10201 catctgatgt tcctgtggac tttaatactg aaggtgttgg aggaccaagt gcaggtttaa
    10261 tgtttacttt agcgatttac gaccaattag ttaaagaaga tttacgtaaa ggacgtaaga
    10321 ttgcaggcac tggtactatt gagcaaaatg ggcatgttgg cgatattggt ggagcaggtt
    10381 tgaaagttgt ttcagcggct aaaaaaggaa tggatatttt ctttgttcct aataatccga
    10441 tcgataaaaa tgctaaaaaa ggcaaaacaa aggttcagac gaattatcaa gaggcaaaag
    10501 cagcagctaa gcgattaggt acgaagatga aaattgtacc agttcaaaat gttcagcaag
    10561 ctattgatta tttgaaaaaa acaaaataaa ctcaaagagt tcgggcgtct gctcggcttt
    10621 tttgttttgc gacatttaac tatcaaagtg ctaaaatgat agtatgacag agcttataag
    10681 aatactacat ttaaatgatc tgcactctca ttttgagaat tttcctaagg taaaacgatt
    10741 ttttcataac aatcaagcgc agccaataga gacgatttca cttgacttgg gggataatat
    10801 tgataaaagc catcctttga cagaagcaag ctcgggcaag gctaatgtcc aattgatgaa
    10861 tgaactgggt attgagcttg ctactattgg taataatgaa ggagttggtt tatccaaaaa
    10921 agacttggac caagtctaca aagattcaga ttttacggtt attgttggta atctaaaaga
    10981 taacataatc gaaccgagtt gggcaaaacc ttacattatt tatgagacac agcaaggaac
    11041 taagcttgct tttttagctt atacttttcc ttattataaa acctatgaac ctaatggatg
    11101 gacgatagaa gatccaattg actgtcttaa atgccattta caaataaatg agataaaaga
    11161 ggcaaattgt cgtattttga tgagtcattt aggtattcgt tttgatacaa ggattgctca
    11221 agaattttca gaaattgacc ttattatagg tgcacataca caccatttat ttgaggaagg
    11281 ggagctaatt accggtacat atcttgcagc agctggtaaa tatggtcgct ttgttgggag
    11341 tattgatata acttttgata atcatacact aaaagatatt ttaatctcaa catatgatac
    11401 aaaacaacta actggttatc cttcggacag tgattgggct aaggagattg agtcagaagg
    11461 taaagaacag cttagaaaag aaagtttaat atcctttcca gcaccattat cattagaaga
    11521 atcgtgtcaa ctagtcatgg atgcgatgct gttttacgca ggtgcagatg ttgctattat
    11581 caattctggc ttaattgttc agccatttga aaaagacttt tcgaggaaaa atctgcatga
    11641 atcattacct catcaaatgc gtctcgctaa attgacagtg agtagtcaag aacttttaga
    11701 gatttatgaa actatttatc aacaaggtca atttttagcg caacaaaaga ttcatggtat
    11761 gggctttcgt ggcaagtact ttggagaagt tttacattcg ggatttgatt acaaaaatgg
    11821 aaaaatagtg tataatgaga aagatattga tgccaaagaa gaagtgattc ttgtaatcgt
    11881 tgatcagtat tattttgcat cgtattttga gtgtttaaaa acaaaaaaag tagacttgct
    11941 ttttcctgaa ttactcagag atgtggtagc agattattta ataaatatga cactataaat
    12001 gaaaaataga taaataaaga gtagaagcta gtcgtttctc tcaatgattt tagagaaagg
    12061 agtattatgc gaaaagaagt gacaccagag atgcttaact ataataagta tcctggccca
    12121 cagtttattc actttgaaaa tatcgttaaa agtgatgata ttgaatttca acttgttatt
    12181 aatgaaaaat cagcttttga tgtgactgtc tttggacaac gtttttctga gattttatta
    12241 aaatatgatt ttatcgttgg cgattggggt aacgagcagt tgaggctaag aggcttttac
    12301 aaagatgcta gtacgattag aaaaaatagc cggatttcac gtttagaaga ttatattaaa
    12361 gagtattgta actttggttg tgcttatttt gtgttggaga atccaaatcc tagagatatt
    12421 aaatttgatg atgaaagacc tcataagcgt cgtaagtcaa gatccaaatc acaatcatca
    12481 aagtcacaaa ctagaaataa tcgttcccag tcaaatgcca atgctcattt tacaagtaaa
    12541 aagcgtaaag acacaaaacg ccgtcaagaa cgtcatatta aagaagagca agataaggaa
    12601 atgacctctg caaagcagca ttttgttatt cgtaagaaaa aataaaaaaa ggataaactg
    12661 taatcgttta ttccaccatg aagaaagaaa atattatgcc taaaaaaact gatacacccg
    12721 actataaacc ctctatttat agcttaactc gcgatgaact aatagcttgg gctattgaac
    12781 atggagaaaa gaaattccgt gcttcacaaa tctgggactg gctctataaa aagcgtgtgc
    12841 aaagcttcga tgaaatgact aacatttcca aagattttat tgcacttttg aatgagaatt
    12901 ttgttgtcaa tcctctaaaa caacgtattg ttcaagaatc tgctgatgga actgttaaat
    12961 acctttttga attacctgat ggcatgctga ttgagacggt tttgatgcgt caatattatg
    13021 gtttatcagt atgtgtaacg actcaagttg gttgtaacat tggttgcact ttctgtgcaa
    13081 gtggtttaat aaagaaacaa cgtgatctta acaatggcga aatcactgct caaattatgc
    13141 ttgttcaaaa atattttgat gaacgtggac aaggtgagcg cgttagtcac attgtggtca
    13201 tgggaatagg tgaacccttt gataactata ccaatgtatt aaaattcttg cgtacagtta
    13261 atgatgataa tggattagct attggtgcac gtcacattac agtgtctact tcaggtttag
    13321 cacataaaat tcgtgaattt gcaaatgaag gtgtacaagt taacttagca gtatctcttc
    13381 atgctccaaa taatgaatta cgatcaagta tcatgcgtat taatcgttct ttccctcttg
    13441 aaaagttgtt tgcggctatt gaatattata ttgagacgac taaccgtcgt gtaacatttg
    13501 agtatattat gttaaatggt gtcaacgata ccccagaaaa tgctcaagag ttagcagatt
    13561 taacgaaaaa gattcgcaaa ttatcttatg tcaatttaat tccttacaat cctgtctcag
    13621 aacatgacca gtatagtcgt agtcctaaag agcgtgtgga agctttctat gacgttttga
    13681 agaaaaatgg agttaattgt gttgttcgtc aagaacatgg tacggacatt gatgcagctt
    13741 gcggacaatt acgttcaaac acaatgaaac gcgatcgtca aaaagcgaag gtagggcggt
    13801 aatgcttaaa agattattta ctgaagatgg ggaattgaca aagattagtc gtcgtttcgt
    13861 ttggatgtta gtggttatct attgtcttat tattgtcagg atgtgttttg ggcctcaaat
    13921 tatgattgag ggggtatcaa ctccgaatgt tcagcgcttc ggaagaattg tagctctttt
    13981 agtaccattt aattcttttc gtagtttaga tcagctaact agctttaaag agattttttg
    14041 ggttattggt caaaatgtag tgaatatttt actgctgttt cctctcatta tagggttact
    14101 atccctaaag ccaagtttac ggaaatataa aagcgttata ttacttgctt tcttgatgtc
    14161 tcttttcata gagtgtactc aagttgtttt agatatttta atagatgcta atcgggtttt
    14221 tgaaatcgac gatctatgga caaatacctt aggcggtcct ttcgccctat ggagttatcg
    14281 aaacataaaa ggttggcttc taactattag aaaatgaaaa taatgtaaag gctatgatat
    14341 tgttcaagcc ttttactatt taaaaaggat cactttagtt gacgaaagta agcttataga
    14401 ggcataatag agagttgtta aatgagaatt ttaaaacact ttttatgtct tctgattgat
    14461 ttggtcttct gacttaatta tgatgttttt caacctttga gaaaggaaaa atatgtctat
    14521 tataaaaaat ttgtggtggt tttttaagga agaaaagaag cgctatctta ttgggatttt
    14581 gtcattaagt ttggttgcag tgttaaatct tatccctcct aaaatcatgg gatcagttat
    14641 tgatgctatt acaactggaa aattaacaag accacaatta ctatggaatt tattaggttt
    14701 ggttttgtca gctttagcta tgtatgggct gcgttatatt tggcgtatgt atattttagg
    14761 gacttcttac aaattaggcc aagttgtcag ataccgttta tttgaacatt ttacaaaaat
    14821 gtctccttct ttttatcaga aatatcgtac aggtgattta atggcgcacg cgaccaacga
    14881 catcaattct ctaacacgtc ttgcaggagg aggagttatg tcagcagtgg atgcctctat
    14941 cacagcatta gtaacgctta tcaccatgtt ctttactatt tcgtggcaaa tgacattaat
    15001 tgcggttatc cctttgccct taatggcctt agcaactagt aaattggggc gaaaaaccca
    15061 tgaaaccttc aaagaatctc aggcagcctt ttcagaatta aataataaag tgcaagaaag
    15121 tgtctctggc gtcaaagtga ctaaatcatt tggttatcaa gaacaagaaa ttgcttcttt
    15181 tcaagagatt aatcagatga ctttcgtgaa gaacatgcgg accatgactt atgatgtcat
    15241 gtttgatcct ttagttcttc tttttatagg tgcctcctac gtattaacat tggctatggg
    15301 agcttttatg atttcaaaag gtcaagttac tgttggtgac ttggtaacat ttgtgacgta
    15361 tttagatatg ttggtatggc ccttgatggc gattggtttc ttgttcaata tggtacagcg
    15421 tggtagtgtt tcttataacc gtattaatag tctacttgag caagaatcgg atataactga
    15481 tcctttaaat cctatcaaac ctgttgtcaa tggaacatta agatatgata ttgatttctt
    15541 tagatacgac aatgaggaaa ccttagccga tattcatttc accttagaaa aaggtcaaac
    15601 cttaggtttg gtaggtcaaa cgggatcagg gaagacaagt cttattaagt tattgctacg
    15661 tgaacatgat gtgactcagg ggaaaattac tttaaataaa catgatatac gtgattatcg
    15721 attgtctgag ttacgtcaac taatcggtta tgttcctcaa gatcagtttt tatttgctac
    15781 cagtatttta gaaaatgttc gctttggaaa tccaactcta tctatcaatg ctgtcaaaga
    15841 agcaactaaa ttggcacatg tttacgatga cattgaacag atgccagcag gatttgagac
    15901 tctaattgga gaaaaaggag tctcattatc tggtggacaa aaacaaagga ttgcgatgag
    15961 tcgtgccatg attttagatc cagatattct tattttggat gattctctat cagcagtgga
    16021 cgctaaaacg gaacatgcta ttgttgagaa tcttaaaacg aatcgtcaag ggaaatcgac
    16081 tattatttca gcacatcgtt tatcagctgt tgtgcacgca gaccttatct tagttatgcg
    16141 agacggcaga gtcattgagc gaggtcaaca tcaagagttg ctaaataaag gtggttggta
    16201 tgctgaaacg tatgcctcac agcaattaga aatggaggaa gcatttgatg aagtctaatc
    16261 aatggcaagt ctttaagaga ttaatctcct atttacgccc ttataaatgg tttacagtat
    16321 tagctctatc tctcttattg ttgacgactg ttgttaaaaa tattattcct ttaattgctt
    16381 cacattttat tgatcactat ctgacaaatg ttaatcaaac agcagttctt attttagtgg
    16441 gatattattc aatgtatgtc ttgcagacct taattcaata ttttgggaat ctcttttttg
    16501 cgcgtgtttc ttatagtatt gttagagata ttcgtagaga tgcttttgct aatatggaaa
    16561 ggctaggcat gtcttatttt gataggacac cggcaggatc tattgtgtca cgtattacta
    16621 atgatactga agcaatatct gatatgtttt cgggtatttt atcaagtttt atctcggcga
    16681 tatttatttt tacagttact ctgtacacta tgttgatgct agacattaaa ctaacaggac
    16741 tcgtcgctct tttgttacct gttatcttta tattagtgaa tgtctatcgg aaaaaatcag
    16801 tcactgtcat tgctaaaacg agaagtttac ttagtgatat caacagtaaa ttatcagaaa
    16861 gtattgaagg aattcgcatt gtacaggctt ttggtcaaga agagcgcttg aagactgaat
    16921 ttgaggaaat taacaaagag catgttgtgt atgccaatcg ttctatggct cttgatagtc
    16981 tcttcttaag accggcgatg tctcttttaa aactcctagc atatgctgtt cttatgtctt
    17041 attttggatt tacaggagtt aaaggaggtc ttacggcagg attaatgtat gcttttattc
    17101 agtacgttaa tcgtctattt gaccctttaa ttgaagtaac gcaaaatttt tcaaccttac
    17161 aaacatcaat ggtatcagca gggcgtgtgt ttgatctgat tgatgaaaca ggttttgaac
    17221 caagccaaaa aaatacagaa gcttttgtga gagaaggaaa tatcgaattt aaaaatgtat
    17281 cattctcata tgatggaaaa aaacaaatcc ttgataatgt ttcttttagt gttaaaaaag
    17341 gtgaaactat tgcttttgta ggagcgacag gttctggaaa atcatctatt attaatgtct
    17401 ttatgaggtt ttacgaattt caatctggac aagttttatt agatgggaag gatattaggg
    17461 attactcaca agaacaattg cgaaagaata ttggactcgt cttacaagac ccctttttat
    17521 atcatggcac tattaaatct aatatcaaaa tgtaccaaga catcaccgat caagaggtac
    17581 aggacgcagc agagtttgtt gatgctgatc aatttattca gaagttgcca gataagtacg
    17641 attcagctgt ttcagaacgt gggtcaagtt tttcaactgg acaacgccaa ctattagcgt
    17701 ttgccagaac tgtggctagt aaacctaaaa ttttaatctt agatgaggcg acagcaaata
    17761 ttgattcgga aacagagcag attgtacaag attcgttagc aaaaatgcgt caaggtcgaa
    17821 caacaattgc cattgctcac cgcttatcaa ccattcaaga tgctaattgt atctatgttc
    17881 ttgatagagg aaagataatt gaaagtggga atcacgagtc tttattagac caaaaaggga
    17941 cttattatcg tatgtaccaa ttacaagccg gcatgatgga ggtctagaaa atttaggcta
    18001 ttcaaaaggc ataatctgtg aggcttagag gttggaatag ttattcctct aagccttttt
    18061 tatattttac aattagtgta taatgaggtc aaagtacaag gaaaggtcta taaaatgtta
    18121 ctattggtag ataattacga ttcttttacc tataatttaa agcaatattt aagtgtctat
    18181 aaagaaatat tcgtcataaa aaatgatgat cctaaccttt ttttattagc tgagagcgca
    18241 gaagctatcg tcttatcacc tggaccagga cacccaaagg atgctgggaa aatattagaa
    18301 ttaatcaatc agtttatagg acagaaacct attctaggta tctgtttggg gcatcaagcg
    18361 cttgctgagt gcttgggtgg gcgattaaat ttagcaaatc acgttatgca tggtaaacaa
    18421 agttgggtaa caataaacga ccatactagt ttgtttaagg gaattgatag tccaacccaa
    18481 gttatgcgtt atcactcatt ggtagtgact gatttaccag agaatattgc tattattgca
    18541 cgatcaaatg aggataatga aatcatggca ttccattgcc ccagcttaaa ggtatatgcg
    18601 atgcagtttc atcctgagtc aatcgggagt attgatggaa tgaaaatgat tgaaaacttt
    18661 ttgacgttaa ttaatgacta aaatgtgtta gtgtaggata atgactctct ataaataaaa
    18721 gacagtacct aagggcactg tcttttattt agttgtatag atgagttaat ctgtctgcaa
    18781 atttacgata aataatcaag caaatggatg cttttaaggt atccccagga ataaaaataa
    18841 ggttagaaaa tagtgattta gttaagggga gagaagtttg aaaggaaaga acgatgctac
    18901 cacaaatatc aatcaatagt acgccaaata tccaaataat aaggaattaa acccagagtt
    18961 ttgttgtttt cactttctca agccctaggc cgataaaaaa agctgcaaat ggataagtca
    19021 gcagatagcc tgctgttggc ccaaacagcg ttacgagacc tgaccgccct cctggtaaaa
    19081 tggtgcacca atagcaacta agagcaagaa aatagccaca gctaggaaac ctttcctact
    19141 accaagcaga gcacctgcca gcataacacc aagattttgt aagacaattg ggactggaat
    19201 gaaacctaat gggatccctg ggataaaacc taaaacaatg agtagtgcaa ccatgagtgc
    19261 aatataggta gttgttcttg ttttcattta aattaattcc tttttagtat gatttagttg
    19321 taatttttcc aagaattcaa tatcggtatg ttgattgcga cccccctctg ttaggtaatt
    19381 tcccaaaaaa gttgagtcaa caagtaaggt gactaatgat tcaaaattct caaaatggac
    19441 ctcccgtcca gcacttaatc ttaattcctt gaaaggaaaa acaaaacgca acatggccat
    19501 aatttttaag catttaatgg gagtcaaata gttatatttt ccaagaggtg ttccttcaac
    19561 agcaagtaaa aaattgacag ggatagaata ggggttcagt tcttttagtc tgaaagctaa
    19621 tgtgatgagc ccctcatcgc tctctcccat accacaaatg aatccagaac aaacatcaat
    19681 gtcttcattg tgaattcttt ctaaagtatc acaacgatct ttgaagctat gcgtcgttgc
    19741 aatgttgggg tagttttctt cgggggtgtt gaggttatga ttgatacggt caattcctgc
    19801 ttgttttagc tgatgaagtt gttctctatc caaaaaacca gcggtaaggc atatttctag
    19861 aggaaggctt tttttgattt cagggatgac ttgacataac tgctcaattt ctctcttgct
    19921 aggcttagtt ccactcatcg caatacaaaa gacgctagca ccattttgat gagcgacaat
    19981 agcctgtttt agaatgacat tttgaggaag cagaccaaat ttatcaatgt cagcagtgct
    20041 ttctttagat tgagaacagt aaccacaatt ttctgcacaa agtccttgct tagcacttaa
    20101 taaaacgtta aggcgaatat tatttcggct aacttgattt ttaaggtata aggcagcagc
    20161 gtagatttcc caccagttct catcgctatt tagaatttcc agcgcttgtt cataagaaat
    20221 gctagtcttt ccagataata tttcgtcagc caaatggata taatttgttt gaaatgacat
    20281 gatagcctcc tttaacgaaa ttaataatag cataaaaaaa ctgtattgta aacttagtct
    20341 atataataag ttaacaatat aaaaaattat gatatactgg tcttatattt tgacgggaga
    20401 taagatgaaa aaactattta aaaaagcgat agctatgtgc cgagatttgc cgcttaattt
    20461 tgtaattgtt gaacttagtt tgtatcaaag agaggatggc tcatctgata atattaaatg
    20521 gatttctaag cgaatccttg aggtaacggt aactgatcag caatggattt accatttgag
    20581 gatgcttaat ttaccaaaac ttaagccaat gtcactagca tccagtgact gtcctgcaag
    20641 ggaagagaag gctagtctta tttcaataga agaatgtcaa aaattcacta gcttagtgcg
    20701 agataaaaat ccttttcacc aaggggaagc agctattgtg cctgcggctc tattattgga
    20761 aaaaataatg gaagttagaa atggaaaaat aaaggagatt catttgactt ttttatctcc
    20821 agtaagggtg aatcaagaat tctcagtgca agtagtggat aacacagtga ttatttttca
    20881 gcatcaaata ttatgtgtga gggggacata tgaagaatag agatgtttat attggttttg
    20941 ggctacgaac acctatcggt ataaaaggga agcaatttaa acattaccgt ccagaacttt
    21001 taggagcaca cctcttaaat caaataaaaa aaatagaatc agaatctaat attgatagta
    21061 ttatttgtgg gaacacagtt ggtactgggg gcaatattgg tcgtttgatg actctttttt
    21121 ctgattatga atcctatatt ccagtacaaa cgattgatat gcagtgtgct tcatcaagtt
    21181 ctgccttgtt ttttggttat ctaaaaatca gtgccggtat taatgaaaaa gttcttgttg
    21241 gggggattga aagtagttct cttcaaccta tgagacgtta cgctaaagaa gataatcgta
    21301 acggagaata taccgttgct cagttttctc ctgactctta tgctgaaact gtaatgttag
    21361 aaggggcaca aagagtctgt caaaaatatg gttttagaag agaaatgtta gataaattgg
    21421 cattcttgag ccataaacgc gccttaacag ctaaacaagg tggctattta gaagaggtaa
    21481 tcttaccaat ggaagggatg cgagatcaag gcgttagaaa actaaaagaa gcattttttc
    21541 aaaaattacc aagattgatg gaaaattcac ctttgctcac tattggaaat gtttgtttaa
    21601 tgcacgatgc tgctgcattt ctaacgcttc agagtcagaa aacagaattc cggattgttc
    21661 acattgtaga agtagcagga gatcccaagc ttagtccaga attggttcac acggctacgg
    21721 aaaaactatt aacagaaact catactaaaa tatcggatta tgatgcaatt gaatggaatg
    21781 aaccatttgc agcgattgat gctttattta atcattatta tcctgaagag agagaaaaat
    21841 tcaatatttt tggaggggca ttagcttacg gacaccctta tgcctgctca ggaattatta
    21901 atatccttca tcttatgcag gcattaaaat ataaaaataa acctatgggt ctaactgcca
    21961 ttgcaggggc aggaggagtc ggaatggcca tatcaataga gtacttagga gttaagaatg
    22021 cttgaatcat tgaaaacaat agttaaaacc aatagcgata aaaagttatt tgatggtgat
    22081 ttacaggtca gttatggaga attttataac ttagtcagac aagatatggc gtcacaagac
    22141 aataggaaac atgtgattag cacacactct ctgcttaacc aattagtaag atttgtttca
    22201 aagttatgtc aaaaagctct tcctattatt tgtaaaccta accttactca taatgaaata
    22261 agtcgattag aaaaagaagt gcagtatgct cctcaactag ctgattttgg tgttctttct
    22321 tctgggacta cagctgatgc taaactattg tggaggagct tcgcttcgtg gtctgatttt
    22381 tttagcattc agaatgctta tttttcggtc acttccaata gcagactgtt tattcaaggt
    22441 gatttttcat ttactggtaa cttaaattta gcgctatccc ttttattact ggggggaacg
    22501 cttgttgtca cacagaaaaa ttctgtaaaa tactggcaga ctttatggga aaaaactggt
    22561 gtaacgcatc tatatctgct ccctagttat ctcaagttag tggaacaata tagcaaggag
    22621 actgcgttag acaataaaac tattattacc tcttcgcaat atgtctctga ctcattactg
    22681 gaaggtttat atataaagca tcctaaagtg tcagtgaaaa tcttctatgg tgctagtgaa
    22741 ctcaattatg tgtcatggta cgatggtcgt gatatccgag ataagccaca gtatgttgga
    22801 gaaatcgttc caaatgtagc tgttaggatt aaagagggaa gaatttttgt aaaaacaccg
    22861 tatagtatct gtggcctttc aagtgaatat tgtgctggtg attatggtga actgatagat
    22921 ggcaaattat atctatttgg tcgtggtgac gattggtgca atcaatcggg cattaagctc
    22981 tatttaccta gattaattga aaaaataaaa acatgccctt atatcaagga tgcagtagca
    23041 tttacaaaag agagtcaaag tcatggacaa gagtcacatt gctgtattgt tttaattgaa
    23101 aatcagatgc aacaagagtg tttgaagtgg ttatcggaac attttgaaaa gaagtatggg
    23161 tttaagcatt atcatattgt atctaaaatt cctttgatgc catctggaaa aattgattat
    23221 caacaactca aaagacaatt agcatgagtt tttactaaaa aaataaaata gggttaatgg
    23281 attgcaaacc tcagttctga tattctttat tttgaaagat atcgtgatat aatgataaaa
    23341 aagaaagata aggaataaaa tgttatcaaa agcaaaatca cgatatatta tccgtgagat
    23401 tattaagtta tttccagatg ctaaacctag cttagacttt actaatgtct ttgagctttt
    23461 ggtagctgta atgctatcag ctcaaacaac tgatgcagct gttaataaag tcactcccgc
    23521 cctttttgaa cgctttccaa atcctttagt tttggcacaa gctgatccca aagagattga
    23581 accctatatt tctaaaattg ggctttaccg taataaagct cgattcttaa atcaatgcgc
    23641 taaacaattg atagagcatt ttgatgggaa agtcccccaa actcgtcaag agctagaaag
    23701 tttgtctggc gttggaagga aaacggctaa tgtggtcatg agtgttggat ttggtattcc
    23761 ggcttttgcc gtagatactc atgtcactcg gatttgtaaa caccaccaga tttgcaaaca
    23821 gtcggcttct ccactagaaa ttgagaaacg agtcatggag gttttaccgc cagaagagtg
    23881 gctagctgct catcaatcca tgatttattt tggtcgtgca atttgtcatc ctaaaaatcc
    23941 aaaatgcgac cagtacccac aactttatca ctttcctgat aatttaaagt gattagaata
    24001 aaagagagta actggtgacg attatataag cgatacttaa aaacggtaga aaagctatcg
    24061 tttttttcat ttttctcata actaaacaat aaataattcc taaccaagca cctatttgaa
    24121 taataaaaag catttgatga agaggtaaag ataagccaat tgtggctaaa taaaggaaat
    24181 ctccacttcc gatattaata tcaaccactt cagctaaaat cccaaataaa cataaaacaa
    24241 tagaaacttt tgtaatcggt gtgagaatta tcgttgaaat ggtgaataat atccatagaa
    24301 aaataggata ctcttggtat tttatatcat aataacttaa gataaaactc atgcataaag
    24361 aaataaaata gatggtagac ataatcataa aatccttttt atagtttata tttatattcg
    24421 aaaaatggta gaaaagagat tatgatttct attatagata gtcagcctaa cgtcataata
    24481 acaattagca agcggttgcg gattttcttg aaaattgaat ggttttctgt taaaattcat
    24541 atatgaaagt tttattcgat gtgcagaatc ttttgaaaaa atttggtatt tatgtttata
    24601 ttggtaaacg tctttatgat atcgaagtta tgaagattga attgcaacgt ctctacgata
    24661 atggtctgat aagtagagat gactatttaa aagcagaact gattttaaga agagagcaca
    24721 gactagaatt ggagaaggaa aataaaaaat gagtaagaaa ttattgggaa ttgacctcgg
    24781 aggaacgacc attaaatttg gtatcttgac gcttgaggga gaagtacaag aaaaatgggc
    24841 aattgagacc aatactttag aaaacggaag acatatcgtt tctgatatcg ttgaatctct
    24901 caaacatcgt ttgagcctct atggattaac aaaagatgac tttctcggta tcggtatggg
    24961 ttctccagga gctgttgata gaactagtaa aacagtaaca ggtgctttta atctaaattg
    25021 ggctgatact caagaagtag gttcggttat tgaaaaagaa gttggaattc cattttttat
    25081 tgataacgat gctaatgttg cagcacttgg tgaacgctgg gtaggtgctg gtgccaataa
    25141 tcccgatgtt gttttcgtaa ccctcggaac aggagtaggt ggaggtgtta tcgcagatgg
    25201 taacctcatc catggtgttg caggagcagg tggagaaatt gggcatatga ttgttgatcc
    25261 agaaaatgga tttacgtgca catgtggtaa caaaggctgt cttgagacag ttgcatcagc
    25321 gacaggtgtt gttagagtag cacgtcaact cgcagaacaa tatgaaggtt cgtctgccat
    25381 taaagcagcg attgacaacg gtgatactgt tacaagtaaa gatattttta tagcagcaga
    25441 agatggggat aaatttgcta attctgttgt tgaacgtgta tcacgttacc ttggactggc
    25501 agcagctaat atttcaaata ttttaaaccc tgattctgtg gttattggtg gcggtgtctc
    25561 agcagcaggt gaatttttac gtagtcgcgt tgagaaatac tttgtcacat ttgctttccc
    25621 acaagttaaa aagtcaacta aaattaagat tgctgaacta ggtaatgatg ctggtattat
    25681 tggtgcagca agcttagcca atcaacaagc aagttaagga gataagatat ggatatgtct
    25741 gttattttaa ttatcgttat tcttctggct tttgttgctt gggcatcttg gaattattgg
    25801 agggttcgta gggcagctaa atttttagat aacgagtctt ttcaaaaaga aatgtcacga
    25861 ggacagttaa ttgatatccg agaagctggt gcttttcata gaaagcatat tctcggagca
    25921 cgtaacattc cagcaagtca atttaaagtt gccttatcgg ctttacgcaa agataaacct
    25981 gtcctcttat atgacgcaag ccgtggtcag tctattccaa ggattgtttt gttactcaga
    26041 aaagaaggtt ttaatcaact ttacgtgtta aaggacggtt tcaattactg gacgggtaga
    26101 gttaagtaat atactaagga gagagttact attaataact ctcttctctt tttgtagaaa
    26161 tgaattaaat tgaaaaaagt agaaaataaa tgacataaat aatgaaatga tgaaaagttt
    26221 tcctatcaca aataggcagt taatatgaaa acatttacac ttgtgtaaat tctgtttttt
    26281 aagaaaaatt gtgttataat tcataagtta acagaattac attataaaat agaggaaaac
    26341 atgacaaatt taagaacaga tatccgtaac gttgcgatca ttgcccacgt tgaccacggt
    26401 aaaacaactc tcgttgatga attattaaaa caatcacata ctcttgatga gcgtaaagag
    26461 cttgaagaac gtgcaatgga ttcaaatgat atcgagaaag aacgtggtat caccattctt
    26521 gcaaaaaata cagccgtagc atacaacgat gttcgtatca atattatgga cacacctggt
    26581 cacgcggact ttggtggtga agttgagcgt attatgaaaa tggttgatgg tgttgtttta
    26641 gtcgttgatg cttacgaagg aacaatgcca caaacacgtt ttgttttgaa gaaagctctt
    26701 gaacaaaact taattccaat cgttgttgta aataaaattg ataagccatc agctcgtcca
    26761 tcagaggttg ttgatgaagt tcttgaactc ttcattgagc tcggtgctga tgatgaccaa
    26821 ctagatttcc ctgttgttta tgcttcagcc atcaatggaa catcttcaat gtcagatgat
    26881 ccttcagatc aagaaaaaac aatggcaccg atttttgata ctatcattga tcacattcca
    26941 gccccagttg acaactcgga agaaccactt caattccaag tttctcttct tgattacaat
    27001 gattttgtag gacgtattgg tattggacgt gttttccgcg ggactgtcaa agttggagat
    27061 caagttactc tttcaaaact tgatggtaca actaaaaact tccgcgtaac aaaacttttt
    27121 ggtttctttg gacttgaacg taaagaaatc caagaggcta aagcgggtga tttaatcgct
    27181 gtttctggta tggaagatat cttcgttggt gagacagtaa ctccgacaga tgctattgaa
    27241 ccactaccag ttttacgtat tgacgagcca acacttcaaa tgactttctt ggtgaataat
    27301 tcaccatttg caggtcgcga aggtaaatgg attacgtcac gtaaggttga agaacgtctt
    27361 ttagcagaat tacaaacaga cgtttctttg cgtgttgacc caacagattc gccagataaa
    27421 tggacggttt cagggcgtgg agaattacat ttatctatcc tcattgaaac aatgcgtcgt
    27481 gagggatatg aacttcaagt atcacgtcca gaagttatca tcaaagaaat tgatggtgtt
    27541 caatgcgagc cgtttgagcg tgttcaaatt gatactccag aagaatatca gggtgctatt
    27601 atccaaagtt tgtcagagcg taaaggtgat atgcttgata tgcagatggt tggtaatggt
    27661 caaacgcgtt tgattttctt gattcctgca cgtggtttga ttggttattc aacagagttt
    27721 ctttcaatga cacgtggata tggtatcatg aatcatactt ttgaccagta tctaccggtt
    27781 gttcaaggag aaattggtgg tcgtcatcgt ggtgtcttgg tttctattga aaatggtaaa
    27841 gcaactacat attcaattat gcgtattgaa gaacgtggga ctatctttgt aaatccaggt
    27901 acagaagttt atgaaggaat gattgttggt gagaattctc gtgataatga tctcggagtc
    27961 aatattacaa ctgctaaaca aatgacaaat gtccgttcag caactaaaga tcaaactgca
    28021 gtcatcaaga cgcctcgtat ccttacttta gaagagtcac ttgaattttt agcagatgat
    28081 gagtacatgg aagtaactcc tgagtctatt cgtcttcgta aacaaatttt aaataaagca
    28141 gcacgtgaca aagctaataa aaagaaaaaa tcagctgaat aatatccaaa gtatttctta
    28201 tttatgatta ataagaaagg agttctatgt tttacttaat tgttgccctt ttgattgcct
    28261 tatattactt ttttatggca ccaaaaacag tacgtaatac tttgaacgct attggtttag
    28321 taggtttggt agccttatta cttgtcttgg cagttatgag ttttattaaa atccttcaat
    28381 tgccaggtga attgtatatt gggcttataa tgattcctct aggatataca gcatttaaag
    28441 aaacattgaa tttgtcagaa aagaagaaat agcttatgaa cttaggattt tgtctcctaa
    28501 gttcttttgt tttgagaaat tagagacata tcctttacct tgaaaaagcc actgattaaa
    28561 ggtaaaataa tagggataat aatgatagaa tgggacgagt aatgaaaaca ataacaacat
    28621 ttgaaaataa aaaagtttta gtccttggtt tagcacgatc tggagaagcc gctgcacgtt
    28681 tgttagctaa gttaggagca atagtgacag ttaatgatgg caaaccattt gatgaaaatc
    28741 caacagcaca gtctttgttg gaagagggta ttaaagtggt ttgtggtagt catcctttag
    28801 aattgttaga tgaggatttt tgttacatga ttaaaaatcc aggaatacct tataacaatc
    28861 ctatggtcaa aaaagcatta gaaaaacaaa tccctgtttt gactgaagtg gaattagcat
    28921 acttagtttc agaatctcag ctaataggta ttacaggctc taacgggaaa acgacaacga
    28981 caacgatgat tgcagaagtc ttaaatgctg gaggtcagag aggtttgtta gctgggaata
    29041 tcggctttcc tgctagtgaa gttgttcagg ctgcggatga taaagatatt ctagttatgg
    29101 aattatcaag ttttcagcta atgggagtta aggaatttcg tcctcatatt gcagtaatta
    29161 ctaatttaat gccaactcat ttagattatc atgggtcttt tgaagattat gttgctgcaa
    29221 aatggaatat ccaaaatcaa atgtcttcat ctgatttttt ggtacttaat tttaatcaag
    29281 gtatttctaa agagttagct aaaactacta aagcaacaat cgttcctttc tctactacgg
    29341 aaaaagttga tggtgcttac gtacaagaca agcaactttt ctataaaggg gagaatatta
    29401 tgtcagtaga tgacattggt gtcccaggaa gccataacgt agagaatgct ctagcaacta
    29461 ttgcggttgc taaactagct ggtatcagta atcaagttat tagagaaact ttaagcaatt
    29521 ttggaggtgt taaacaccgc ttgcaatcac tcggtaaggt tcatggtatt agtttctata
    29581 acgacagcaa gtcaactaat atattggcaa ctcaaaaagc attatctggc tttgataata
    29641 ctaaagttat cctaattgca ggaggtcttg atcgcggtaa tgagtttgat gaattgatac
    29701 cagatatcac tggacttaaa catatggttg ttttagggga atcggcatct cgagtaaaac
    29761 gtgctgcaca aaaagcagga gtaacttata gcgatgcttt agatgttaga gatgcggtac
    29821 ataaagctta tgaggtggca caacagggcg atgttatctt gctaagtcct gcaaatgcat
    29881 catgggacat gtataagaat ttcgaagtcc gtggtgatga attcattgat actttcgaaa
    29941 gtcttagagg agagtaacta tggggaaaaa aattgttttt acaggtggtg gtacggtagg
    30001 tcatgtaaca ctaaacctaa tattaatccc caaatttatc aaagatggtt gggaagtaca
    30061 ttatattggt gataaaaatg gcatagaaca tgaacaaatt aatcagtctg gacttgatat
    30121 cacatttcac tctatagcta ctgggaaact acgtcgctat ttttcatggc aaaatatgtt
    30181 agatgtcttt aaagtagggg ttggcgttct tcaatcgatt gctattattg ctaaacttag
    30241 accccaagcc ttattttcaa aagggggatt tgtttcagta cctcctgtag tagctgcacg
    30301 cctattaaaa gttcctgttt ttgttcatga atcagattta tcaatggggt tagcaaataa
    30361 gattgcttat aaatttgcga ctattatgta tacgactttt gaacaatcta aagatttaat
    30421 caaaactaag catattggtg ctgtaacaaa agtcatggat tgtaaaaagt cgtttgaaaa
    30481 tactgactta actagtataa aagaagcctt cgatccaaac ctaaagacct tactatttat
    30541 tggaggatca gcaggtgcaa aagtattcaa tgattttatt actcaaacac ctgagctgga
    30601 agaaaaatat aatgttatca atatttcagg cgattcttct ctcaatcgat tgaagaagaa
    30661 cctttatcgc gtggattatg ttacagacct ttatcagcct ttaatgaatt tagcagatgt
    30721 ggttgttaca cgaggtggct ctaatactat ttttgaatta gtagctatga aaaaacttca
    30781 tcttattatc cctcttggac gtgaagcgag ccgaggtgac caattagaga atgctgctta
    30841 ttttgaagaa aagggctacg cattgcagtt accagaatct gaattaaaca taaatacact
    30901 tgagaaacag ataaatttat taatttctaa cagtgagagt tacgaaaaaa acatgtcaca
    30961 atcatctgaa ataaaatctc aagatgaatt ttatcaatta ttaattgatg atatggcaaa
    31021 agtaacaaaa ggataaaaca tgcctaagaa gaaatcagat accccagaaa aagaagaagt
    31081 tgtcttaacg gaatggcaaa agcgtaacct tgaattttta aaaaaacgca aagaagatga
    31141 agaagaacaa aaacgtatta acgaaaaatt acgcttagat aaaagaagta aattaaatat
    31201 ttcttctcct gaagaacctc aaaatactac taaaattaag aagcttcatt ttccaaagat
    31261 ttcaaaacct aagattgaaa agaaacagaa aaaagaaaaa atagtcaaca gcttagccaa
    31321 aactaatcgc attagaactg cacctatatt tgtagtagca ttcctagtca ttttagtttc
    31381 cgttttccta ctaactcctt ttagtaagca aaaaacaata acagttagtg gaaatcagca
    31441 tacacctgat gatattttga tagaaaaaac gaatattcaa aaaaacgatt atttcttttc
    31501 tttaattttt aaacataaag ctattgaaca acgtttagct gcagaagatg tatgggtaaa
    31561 aacagctcag atgacttatc aatttcccaa taagtttcat attcaagttc aagaaaataa
    31621 gattattgca tatgcacata caaagcaagg atatcagcct gtcttggaaa ctggaaaaaa
    31681 ggctgatcct gtaaatagtt cagagctacc aaagcacttc ttaacaatta accttgataa
    31741 ggaagatagt attaagctat taattaaaga tttaaaggct ttagaccctg atttaataag
    31801 tgagattcag gtgataa
//
LOCUS       DATRSG010000013        31498 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRSG010000013 DATRSG010000000
VERSION     DATRSG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31498
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.013"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(23..1804)
                     /locus_tag="VBD89_000636"
     CDS             complement(23..1804)
                     /locus_tag="VBD89_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO7135188.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRSDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGYFASYIPSLAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            1974..2576
                     /locus_tag="VBD89_000637"
     CDS             1974..2576
                     /locus_tag="VBD89_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7135189.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            2623..4029
                     /gene="pepV"
                     /locus_tag="VBD89_000638"
     CDS             2623..4029
                     /gene="pepV"
                     /locus_tag="VBD89_000638"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO7135190.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            4126..4710
                     /locus_tag="VBD89_000639"
     CDS             4126..4710
                     /locus_tag="VBD89_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO7135191.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            4725..5459
                     /locus_tag="VBD89_000640"
     CDS             4725..5459
                     /locus_tag="VBD89_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135192.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            5628..9386
                     /locus_tag="VBD89_000641"
     CDS             5628..9386
                     /locus_tag="VBD89_000641"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO7135193.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQLGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDVAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            9521..10439
                     /locus_tag="VBD89_000642"
                     /pseudo
     CDS             9521..10439
                     /locus_tag="VBD89_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
     gene            complement(10481..10657)
                     /locus_tag="VBD89_000643"
     CDS             complement(10481..10657)
                     /locus_tag="VBD89_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO7135194.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            10768..11658
                     /gene="miaA"
                     /locus_tag="VBD89_000644"
     CDS             10768..11658
                     /gene="miaA"
                     /locus_tag="VBD89_000644"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO7135195.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            11749..12987
                     /gene="hflX"
                     /locus_tag="VBD89_000645"
     CDS             11749..12987
                     /gene="hflX"
                     /locus_tag="VBD89_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO7135196.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            12980..13627
                     /locus_tag="VBD89_000646"
     CDS             12980..13627
                     /locus_tag="VBD89_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO7135197.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGEYYYSSQGRESVIYIDTKSTN"
     gene            13669..14598
                     /locus_tag="VBD89_000647"
     CDS             13669..14598
                     /locus_tag="VBD89_000647"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO7135198.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            14600..15361
                     /locus_tag="VBD89_000648"
     CDS             14600..15361
                     /locus_tag="VBD89_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7135199.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            15358..17556
                     /gene="recJ"
                     /locus_tag="VBD89_000649"
     CDS             15358..17556
                     /gene="recJ"
                     /locus_tag="VBD89_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO7135200.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMALGTPKEIYDFMMS"
     gene            17708..20272
                     /locus_tag="VBD89_000650"
     CDS             17708..20272
                     /locus_tag="VBD89_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO7135201.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            20395..20913
                     /locus_tag="VBD89_000651"
     CDS             20395..20913
                     /locus_tag="VBD89_000651"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO7135202.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            21031..21711
                     /locus_tag="VBD89_000652"
     CDS             21031..21711
                     /locus_tag="VBD89_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO7135203.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            21815..22498
                     /locus_tag="VBD89_000653"
     CDS             21815..22498
                     /locus_tag="VBD89_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO7135204.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            22488..23276
                     /locus_tag="VBD89_000654"
     CDS             22488..23276
                     /locus_tag="VBD89_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO7135205.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            23285..24388
                     /locus_tag="VBD89_000655"
     CDS             23285..24388
                     /locus_tag="VBD89_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO7135206.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            24447..25316
                     /gene="rfbA"
                     /locus_tag="VBD89_000656"
     CDS             24447..25316
                     /gene="rfbA"
                     /locus_tag="VBD89_000656"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO7135207.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            25316..25909
                     /locus_tag="VBD89_000657"
     CDS             25316..25909
                     /locus_tag="VBD89_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO7135208.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            26116..27162
                     /gene="rfbB"
                     /locus_tag="VBD89_000658"
     CDS             26116..27162
                     /gene="rfbB"
                     /locus_tag="VBD89_000658"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO7135209.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(27214..30432)
                     /gene="hylB"
                     /locus_tag="VBD89_000659"
     CDS             complement(27214..30432)
                     /gene="hylB"
                     /locus_tag="VBD89_000659"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO7135210.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKAKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            30649..31131
                     /locus_tag="VBD89_000660"
     CDS             30649..31131
                     /locus_tag="VBD89_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7135211.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            31121..>31498
                     /locus_tag="VBD89_000661"
     CDS             31121..>31498
                     /locus_tag="VBD89_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7135212.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELT"
BASE COUNT        10460 a         4866 c         6242 g         9930 t
ORIGIN      
        1 tctactaagc tttttattta atttaaccct cgctatcaat aagttggaga gctctttgat
       61 aataattagg gctatttttg atatctttat taatgacctt gaaaagattg agtttatctg
      121 tgatgattcc attaactggg ctttgtaaat aacgtatcat aaggttatca cggttaatag
      181 tccaaacata gagctgttta ttatattctt gggcgagata tgcaatccaa tcattgtatg
      241 aaaattcctc aatcccataa aagtcaacgt tatgattttg aagcgttccc caatgtagtg
      301 ggattaggta acctgtatca agttgtggca attttttctc aaccttttca atgagtgtca
      361 agttgagaga cattacttta tgcttggttg atagacgaag ctctttaaat tctttgataa
      421 attcatccgc atagatatcg aggttttgat tatacggttt caattcaatc atgatttttt
      481 gatctagtga ctgagcggtc ttcataaatt ctgccaatga tggaatgtaa ctagcaaaat
      541 acccttggtg aattgggatt tttgtaactt gatctaaagt tagtttagaa acatctttgt
      601 tcataccaga gagtcgtttg agattatcat catgaatgac tactaattta tgatcttttg
      661 tcatgataac atccatctct gaataatctg cttttgcttt atgggctgct tttaatgctt
      721 caatagaatt ttcaacgcca tgtgatgtat cactacgatg ggctattaat tcttttttaa
      781 ttgaaatgtt atcttgtaag agagccagag ctatagcatt ttttaaggca gtggtaccaa
      841 tagctagagc tagaataaca caagcaatgt gtttaaattt ttgcttatgg tggacgcctt
      901 tccaagctct ccattgttga acaatacttt tattttgttt aatatctgct aataggtgac
      961 ttatgattaa acatttaata agaagtgtta cggcaaaaag tattgatttt aaaaggttat
     1021 agaaagtggc ttgaagagga aaattgtttc cagaatgatc aatgaatgaa aatgtaccag
     1081 caacaacagc tagtgcaact gttccgatta cagatataaa taaactggtt aaaataattt
     1141 tacttagaag acgccactga cgctttccgc ggctaagttt ccaactctct aaaagtgctt
     1201 ctttagcatt gtagtcagtt aaaatggtca gcgggagaaa ataaattaat cgaaggttta
     1261 agtaacaaat aagcagtaaa agggtagagt aacctatctt tccaaagact gtctttgtta
     1321 actcgccaga aataaagtca ggaattttaa gatgctctaa tatacttgac cttagaccaa
     1381 ggttagctag aggaattgtc aaaagtaagt agattgatac ccaaattagc tgtggtccta
     1441 aaagtgattt tattttttta ggaaagattg ttagccattt tttgatactg agagaaaatt
     1501 cagttttcag tatggcaata gtatagatag cgaactcagt atagatgaac ccaattagga
     1561 ttacgatata aagtataaag agtaataaag ctaagggatg attaaaaagc aaagtccaat
     1621 tgtctttagt cactgcggga gtatttgata atatcaatat ccgacgaaag ataaaactca
     1681 gaattccttt tactaaataa gcaactaaag tttggattaa gataccagtt ccaatgtaaa
     1741 cccaaaattg attttttaaa gaggttaagg ttagaaaaaa atcttctttt aatgctcgta
     1801 gcatgaatag ctcctttttt atagtcatta gatattgtac cacaagtttg tttgtttttt
     1861 atggtagttt tcaaaccggt ttcacttttt tatcaatttt gccttcaaaa gctgaaacgc
     1921 attttaaaaa gtgatagaat agtagggaaa atcaaataga ataggaggtt tttatgaaat
     1981 ttctagaatt aaataagaag cgccatgctg tgaaacattt taatgataag ccagtcgact
     2041 ttaaagatgt tagaactgct attgaaattg cgacattagc gccatctgct aataatattc
     2101 agccttggaa gtttgttgtt gtccaagaaa aaaaatctgc tctagcagaa ggtttaccgg
     2161 aaagtaattg taaccaaatt aatcaagcgc agtatgttat tgctttattt acagatacag
     2221 atttaggaca gagatcacgg aaaatagctc gtattggtcg tagatctctt cctgatgatt
     2281 taattggtta ttatatggaa actctaccac cacgctatgc tttatatagt gaaaaacaaa
     2341 ctggagagta tctctcttta aatgcaggta tcgttgctat gaatttagta cttgccttga
     2401 cagatcaagg tattagttct aatatgattt tagggttcga taaagcgatt accaatgatg
     2461 tcttagagat agataaacgt tttagaccag aaatattaat tactgttggt tatagtgatg
     2521 aaaaggttga gcctagttat cgtttgccag ttgatcatat tattgaaaaa cgttaatttt
     2581 taagcacaaa ctacataata ttttatagaa ggagttgccg taatgacaat tgattttaga
     2641 gcagaagtag acaaacgcaa agacgctttg atggacgatt taataaatct tcttcgtatt
     2701 aactcggagc gtgatgattc acaagctgat gcagagcatc catttggtcc tggacctgta
     2761 aaagccttgg aatttttcct tgagatggct gagcgagatg gctacgaaac aaaaaacgta
     2821 gataattatg cgggacactt tacatttggt caaggcgaag aagaactagg tatttttggg
     2881 catttagatg ttgttcctgc aggcagcggt tgggatacag acccttatga accagttatc
     2941 aaggataacc gtttatatgc tcgagggtca tcagacgata agggaccaac aatggcatgt
     3001 tattatgctc taaaaattat taaagagctt gggctaccaa catctaagaa agttcgtttt
     3061 gttgtaggaa ctgatgaaga atctggatgg ggagatatgg attactactt cgaacatgtt
     3121 ggtcttccaa aacctgattt tggtttctct ccagatgcag aatttccaat tataaatggt
     3181 gaaaaaggta atatcactga atatttacat ttttcaggag aaaataaggg agctgttaga
     3241 ctgcatagct tttctggcgg acttcgcgaa aacatggttc cagaatcagc cacagcacgt
     3301 ttcacaagtc atttagatca gacaacactt ggagctagtc tagctgattt tgcaggtaaa
     3361 cataatttga aagcagaatt atctgtagaa gatgggcagt atatagctac agtttatggt
     3421 aaatctgctc atggttcaat accacaagaa ggtgttaatg gggcgactta tttagctctt
     3481 tatctaagtc aatttgattt tgaaggtcct gctcgtgctt tcttagatgt cacagccaac
     3541 attattcacg aagacttctc aggtgaaaaa cttggagtag cttatgaaga tgactgtatg
     3601 ggatcgttga gcatgaatgc aggtgtcttc cagtttgatg aaactaatga tgataatact
     3661 atcgctctta atttccgtta cccacaaggg acagatgcta aaactatcca aactaagctt
     3721 gagaaactta acggagttga aaaagtgact ctttctgacc atgaacacac accacactat
     3781 gtacctatgg acgatgaatt agtatcaacc ttactagctg tctatgaaaa gcaaactggt
     3841 cttaaaggac atgaacaggt tattggtggg gggacatttg gtcgcttact tgaacggggt
     3901 gttgcatacg gtgccatgtt cccaggagat gaaaacacta tgcatcaagc taatgagtac
     3961 atgcctttag aaaatatttt ccgttcggct gctatctacg cagaagctat ctatgaatta
     4021 atcaaataaa ataatcctta aactaaatat gtgatcaatg ataaagggtg gtgaagacat
     4081 gaaagtgtct ttgcctcttt tcataaggtt agatttggag actttatgac tgacttggaa
     4141 aaaattatta aagcaataaa aagtgattca cagaatcaaa attatacaga aaatggtatt
     4201 gatcctttgt ttgctgctcc taaaacagct aggatcaata ttgttggaca agcacctggt
     4261 ttaaaaactc aagaagcaat actctattgg aaagataaat ctggagatcg tctacgccag
     4321 tggcttggag ttgatgaaga gacattttac cattctggaa aatttgctgt tttgccttta
     4381 gatttttatt acccaggcaa aggaaaatca ggagatttac cccctagaaa aggttttgcg
     4441 gagaaatggc accctcttat tttaaaagaa atgcctaatg ttcaattgac cttgctagtt
     4501 ggtcaatatg ctcagaaata ttatcttgga agctccgcac ataaaaatct aacagaaaca
     4561 gttaaatctt acaaagacta tctacccgat tatttacccc tggttcaccc atcaccgcga
     4621 aatcaaattt ggctaaagaa gaatccatgg tttgaaaaag atctaatcgt tgatttacaa
     4681 aagatagtag cagatatttt aaaagattaa ggataggagt tagtatgaga gataatcatc
     4741 tacacacgca tttttcctat gattgtcaaa cggcatttga ggactatatt aatggtttta
     4801 caggtgaatt tatcacaaca gaacattttg atttatcaaa tccttacacc ggtcaagacg
     4861 atgttcctga ttatagtgct tattgtcaaa aaatagatta tcttaatcag aaatatggaa
     4921 atcgatttaa aaaaggaatt gaaatcggtt attttaaaga tagggaatca gatattttag
     4981 attatttaaa aaataaagaa tttgatttaa aactattgtc aatccatcat aatggtaggt
     5041 atgattatct gcaagaagaa gctctgaaag taccaacaaa ggagctttta gcagattact
     5101 ttaatcgtat ggaatttgcc ataggccgtg tggaagcgca cgttttagat cactttgatt
     5161 atggttttcg taagttaaac ttagatgtag aagatttaaa accgtttgaa acgcaattga
     5221 agcgcatttt cagaaagatg ttatctaagg ggttagcttt tgaactaaat accaaatccc
     5281 tttatctata tgggaatgaa aaactttatc gctatgcttt agagatacta aaacagcttg
     5341 gttgtaaaca atactctata ggctctgacg gtcatattcc tgaacatttt tgttatgaat
     5401 ttgatagact tcaatgtctg ctaaaggact atcaaattga tgaaaatcat ttgatatgag
     5461 gaaatttttg ataaaaaagc taggcaatat tgcttagctt ttttgtaatg tcattgatag
     5521 ttttagtgaa aatttcaaaa aaataaagaa atcacttact tgttgcaagc gcttgcgtaa
     5581 attgttatga ttttattggt aacaattcat taaaaaagga gaatgatatg aaaagaaaag
     5641 acttatttgg tgataaacaa actcaataca cgattagaaa gttaagtgtt ggagtagctt
     5701 cagttgcaac aggggtatgt atttttcttc atagtccaca ggtatttgct gaagaagtaa
     5761 gtgtttctcc tgcaactaca gcgattgcaa agtcgaatat taatcaggtt gacaaccgac
     5821 aatctactaa tttaaaagat gacataaact caaactctga gacggttgtg acaccctcag
     5881 atatgccgga taccaagcaa ttagtatcag atgaaactga cactcaaaaa ggagtgacag
     5941 agccggataa ggcgacaagc ctgcttgaag aaaataaagg tcctgtttca gataaaaata
     6001 ccttagattt aaaagtggca ccatctacat tgcaaaatac tcccgacaaa acttctcaag
     6061 ctataggtgc tccaagtccg accttgaaag ttgctaatca agctccacag attgaaaatg
     6121 gttactttag gttacatctt aaagaattgc ctcaaggtca tcctgtagaa agcactgggc
     6181 tttggatatg gggagatgtt gatcaaccgt ctagtaattg gccaaatggt gctatcccta
     6241 tgactaatgc taagaaagat gattacggtt attatgttga ttttaaatta tctgaaaaac
     6301 aacgaaaaca aatatctttt ttaattaata acaaagcagg aacaaattta agcggcgatc
     6361 atcatattcc attattacga cctgagatga accaagtttg gattgatgaa aagtacggta
     6421 tacatactta tcagcccctc aaagaagggt atgtccgtat taactatttg agttcatctg
     6481 gtaactatga ccacttatca gcatggctct ttaaagatgt tgcaaccccc tcaacaactt
     6541 ggccagatgg tagtaatttt gtgaatcaag gactatatgg aaggtatatt gatgtaccac
     6601 tgaaaactaa tgccaaagag attggttttc taatcttaga tgaaagtaag acaggagatg
     6661 cagtgaaagt tcaacccaac gactatgttt ttagagattt agctaaccat aaccaaattt
     6721 ttgtaaaaga taaggatcca aaggtttata ataatcctta ttacattgat caagtgcagc
     6781 taaaggatgc tcaacaaact gatttaacaa gtattcaagc aagttttaca actctagatg
     6841 gggtagataa aactgaaatt ttaaaagaat tgaaagtgac agataaaaat caaaatgcta
     6901 tacaaatttc tgatatcact ctcgatacta gtaaatctct tttaataatc aaaggcgact
     6961 ttaatcctaa acaaggtcat ttcaatatat cttataatgg taacaatgtc acgacaaggc
     7021 aatcttggga atttaaagac caactttatg cttatagtgg aaatttaggt gcagttctca
     7081 atcaagatgg ttcaaaagtt gaagccagcc tctggtcacc gagtgctgat agtgtcacta
     7141 tgattattta tgacaaagat aatcaaaaca gggttgtagc gactaccccc cttgtgaaaa
     7201 ataataaagg tgtttggcag acgatacttg atactaaatt aggtattaaa aactatactg
     7261 gttactatta tctttacgaa ataaaaagag gtaaggataa ggttaagatt ttagatcctt
     7321 atgcaaagtc attagcagag tgggatagta atactgttaa tgacgatata aaaacggcta
     7381 aagcagcttt tgtaaatcca agtcaacttg gacctcaaaa tttaagtttt gctaaaattg
     7441 ctaattttaa aggaagacaa gatgctgtta tatacgaagc acatgtaaga gacttcactt
     7501 ctgatcaatc tttggacgga aaattaaaaa atcaacttgg tacctttgca gccttttcag
     7561 agaaactaga ttatttacag aaattaggag ttacacacat tcagctttta ccggtattga
     7621 gttattttta tgttaatgaa atggataagt cacgctcaac agcttacact tcctcagaca
     7681 ataattacaa ttggggctat gacccacaga gctattttgc tctttctgga atgtattcag
     7741 agaaaccaaa agatccatca gcacgtatcg ccgaattaaa acaattaata catgatattc
     7801 ataaacgtgg catgggggtt atacttgatg tcgtctataa tcacactgca aaaacttatc
     7861 tctttgagga tatagaacct aattattatc actttatgaa tgaagatggt tcaccaagag
     7921 aaagttttgg agggggacgt ttaggaacca ctcatgcaat gagtcgtcgt gttttggttg
     7981 attccattaa atatcttaca agtgaattta aagttgatgg tttccgtttt gatatgatgg
     8041 gagatcatga tgtggctgcg attgaattag cttataaaga agctaaagct attaatccta
     8101 atatgattat gattggtgag ggctggagaa cattccaagg cgatcaaggt aagccggtta
     8161 aaccagctga ccaagattgg atgaagtcaa ccgatacagt tggcgtcttt tcagatgata
     8221 ttcgtaatag cttgaaatct ggttttccaa atgaaggtac tccagctttc atcacaggtg
     8281 gcccacaatc tttacaaggt atttttaaaa atatcaaagc acaacctggg aattttgaag
     8341 cagattcgcc aggagatgtg gtgcagtata ttgctgcaca tgataacctt accttgcatg
     8401 atgtgattgc aaaatcaatt aataaagacc ctaaggtagc tgaagaagat attcatagac
     8461 gtctgcgttt aggaaatgta atgattttaa catctcaagg gacagcattc attcattctg
     8521 gtcaagagta tggtcgtacg aagcgtttac ttaaccctga ttacatgaca aaagtttcag
     8581 atgacaaatt gcctaataaa gcaacactta ttgaagctgt taaagaatac ccatatttta
     8641 ttcatgattc atatgattct tcagatgcca ttaatcattt tgattgggca gcagccacag
     8701 ataataacaa acacccaatt tcaacgaaaa cacaggccta tacagcaggt ttaatcacat
     8761 taaggcgttc aacagatgct ttccggaaat tgagcaaagc agaaattgat cgtgaggtta
     8821 gcttgattac agaggtaggt caaggtgata ttaaagaaaa agatttggtt attgcttacc
     8881 aaacaataga ttctaaaggc gatatttacg cagtatttgt taatgctgat agtaaagcta
     8941 gaaacgtttt actaggtgaa aaatataaac accttttaaa agggcaagta attgttgatg
     9001 ctgatcaagc ggggattaaa ccaatctcaa ctcctagagg tgttcatttt gaaaaagata
     9061 gtttgctgat tgatccatta acagcaattg tgattaaagt tggcaaagtt gctcctagcc
     9121 ctaaggagga attgcaagca gattatccca aaacacaatc tttcaaggga tctaaaacgg
     9181 tagaaaaagt aaatagaata gctaataaga cctcaataac tcctgtagtt tctaataaga
     9241 ccgattcata tctgacaaat gaagctaatt tgccaaaaac tggagataag tcatcaaaaa
     9301 tactaagtgt agtaggaata agcattctag caagtctact tgctctacta ggtctctctt
     9361 taaagaggaa tcgtcactta aaataaaagc aaactttaga agagcttaaa aaagaagtta
     9421 gtcaatgggc tagcttcttt tcattaagtt aataaaatca cttatcagca aaatctaatt
     9481 gtgttataat ggatgtaaac gtttttttat gaggtatatg atgaaaaatt gggaaaaacc
     9541 accattagtg atggcgggat tagtattggg gctattagct ttggggaacc ttctagaggg
     9601 atatggcacc tattttcgct attgtttagg gctagtagca cttgtttttt ggattttttt
     9661 aatcaaagga atattaaaaa ataaaaaaga aagcaggaag gagttaagca atcctttgat
     9721 tgcttctgtc tttactactt tttttatggc aggtatgatt ttatcgacct atatcttatt
     9781 atttagaagt ttaggaatat gggtagcagt tctgtcaaaa aggtgtatgg tggttgtcat
     9841 ttattgcgtt aatcattcat atggctattt tttcatggaa atatttgaga cacttttcga
     9901 tggctaatct ttttccttct tggtctgtcc tctatgttgg tataggggta gctagcctga
     9961 cagcaccaat tagtggacaa ttcactattg ggaaaatagt tttttggtat ggttttattg
    10021 caactttagt attattacca tttctattca ttaaagcata taaaatcggg ctaccttcgg
    10081 cagttaagcc taatataact actatttgcg ctccaatgtc gctaatcaca gcgggatatg
    10141 ttaatagttt tgtgtcacca aatagaggtt tattattgct attaatcgta atggcacaat
    10201 ttctttattt tttcattctt tttcaggtgc ctaaattact gattggtgat tttacaccag
    10261 gtttctcagc ctttactttc ccccttgtta tctcagctac ctcacttaaa ttaagtattc
    10321 agcatctatc attaccagtt gatatacagg ggctagtcca ctttgaaatt gggacaacaa
    10381 ctcttattgt tatgatagta atggtacgtt atattttctt tttaaggcga acaatttaat
    10441 ataaaataac tcccttacta gctagtaagg gagttattgt ttaatgagag acacgtttac
    10501 gtgcagcttt tttacgattt tcttcgataa aagctatttt ttcttcctca ggttcaataa
    10561 ttgttttttt gatagcaaat gcagcacctg caagagtagc agctgttgca acgactcctg
    10621 ttgctattcc tttagcaaat gcgaattttt taaccataat aattctccaa tctatgatga
    10681 agactctgct tctatctatg atataattat ggtaacgaaa tactagttaa atttcaagta
    10741 aaaaccttta tagaattgag aagtatcatg agaaaaatca agcttatagc agttgttggt
    10801 ccaactgcgg ttgggaagac agcattagga attgaattag ctaaaacttt caatggcgaa
    10861 atcattagtg gcgatagtca gcaagtttat cagaagttag atatcggaac ggctaaagct
    10921 agtaaagaag agcaagaaca agcttatcat cacttgattg atgttcgtga agtaaatgag
    10981 aattattcgg tttatgattt tgtcaaagag gcaaaagtag ctattgatac gattatttct
    11041 aaggggaaaa tccctattat tgttggaggt actggcctat atttacagag tttgtttgag
    11101 ggctatcatt taggaggcga agtcaatcaa gaaactctta aggcctatcg tgaaaaatta
    11161 gagtctctaa gtgatgaaga tttatttgaa aagttgacag aacaatccat tgttataccg
    11221 caagttaatc gtcgacgtgc catccgtgca ttagaattgg cgaagtttgg gaatgatttg
    11281 caaaatagtg aaagtccgta tgatgtttta ttaattggat tgaatgatga tcgtcaggtg
    11341 ctctatgatc gtatcaatag acgagttgat ttgatgatag acaatggtct cttagatgaa
    11401 gctaaatggc tatacgataa ctaccctagt gttcaagcaa gtagaggaat tggctataaa
    11461 gaattatttc cttacttttc aaagcaaata ccattagaag aagctgttga taaactaaaa
    11521 caaaatacca gacgttttgc aaaacgccaa ttaacatggt ttagaaaccg tatgaatgtc
    11581 gaatttatta tggttggtga ggaaaattat caacaaaaaa tcaagcgaaa agtcagtgat
    11641 tttttatcat caaagtaaat aaagccgttt ctcgcattta aagggcatta tgttataata
    11701 aagattacgt atttagaaca agaaaaaaat attgaaagga gattataggt gatagaaact
    11761 aaagaagagc aagaacgtgt tattttagtt ggggtagaac ttcaagatac tgagaatttt
    11821 gaaatgtcca tggaagaact tgctagctta gctaagacag caggagcaaa tgttgttaat
    11881 cattattatc aaaaaagaga taaatatgat agtaaatcat ttatcggatc tggtaaatta
    11941 gaggagataa aagctattgt agaagcagat gagattgata ccgttgtggt taataatcgt
    12001 ttgactccgc gacaaaactc taatttggaa gctgaacttg gtgttaaagt tattgaccgt
    12061 atgcaactta ttttagatat ctttgctatg cgtgctagaa gtcatgaagg aaaattacag
    12121 gttcatctag cgcaattgaa atatatgtta ccacgcttag ttggacaagg tattatgctt
    12181 agtcgtcaag caggaggaat cggaagccga ggtccaggag aatctcagtt agagttgaat
    12241 cgtcgttcca ttcgtcatca aatttctgat atcgaaagac aattgaagat tgtagaaaaa
    12301 aatcgcgaga cagttcgaga aagacgtgtt gattcgacaa cctttaaaat tggattaatt
    12361 ggatatacaa atgctggcaa gtcaactatt atgaatgttt tgaccgatga taagcaatat
    12421 gaagccaatg aattatttgc aacgttagac gctacaacca agcagatcta tttacaaaat
    12481 caatttcaag tcactttaac agatactgtt ggttttattc aagatttacc tacagagtta
    12541 gttgcggctt tcaagtctac cttagaagaa agtcgacatg ttgatttatt acttcatgtt
    12601 attgatgcta gtgaccctaa tcatgaagaa cacgaaaaag ttgttatgga gattcttaaa
    12661 gacttagata tgatagatat tcctcgttta gcgatataca acaagatgga tgtcactgag
    12721 cagctgaatg caacaacttt tcctaatgtg agaattgcag ccaaaaaaca agggagtagg
    12781 gatttattac gccgtcttat tgtggatgaa atacgacata tctttgacga attttcgatt
    12841 agagtgcatc aaaatcaagc atataagctc tatgatctca ataaaatagc attgcttgac
    12901 acctacacct ttgaagaaga atacgagaat attacaggct atatttctcc taaacaaaaa
    12961 tggaagctgg aagaatttta tgattgatta tattgatttg gctttgactt atggtggttt
    13021 tacgagctta gataaagttt atctagagaa aaaactagat ggcctctcta agcaacaacg
    13081 acttgatttt atcactccac ctcctagtgt gattaatgct tattttgctg aaatatatca
    13141 aaagcaaggg ccagaagcgg caactgatta ctattttgat ctttccaaag ccttgggctt
    13201 gtttccaaaa catttatcgt ttgatgaaga aaaacctttt attcgtttga atttatcagg
    13261 aaaatcattt ggttttgctt acctaaatga tcaagaagaa gcgagtgttt tttctgaagt
    13321 taaagaagtc attacaccgc agttactctt agaaattgca cagatttttc ctcaatataa
    13381 agtctatcaa gatcggtctg gtatcagaat ggcaaaaata gattttgacg aaacagaatc
    13441 tcaaaatatt acaccagaaa caagtttact agggaatgtc ttacaactta aaaaagatat
    13501 tattaaaata actagtttca atcaagaaga gctattagag ttggttaaaa caaagtcagg
    13561 tgaatactat tattctagtc aaggacgtga gtcagttatt tatattgata ctaaatctac
    13621 taattaaatt tattaagtta tggaaggatt ataaaaaagt caacaagtat ggaaattcaa
    13681 tttttaggaa ctggagctgg tcaaccagcg aaagctcgta atgtttcaag tttagtcttg
    13741 aaattattag atgaaattaa cgaggtttgg atgtttgatt gtggagaggg tacacagcgt
    13801 caaattttag aaaccacgat taaacctcgt aaagtcaaaa aaatttttat cacgcacatg
    13861 cacggagacc atgtttttgg tcttccaggt tttttatcca gtcgggcttt tcaagccaat
    13921 gaagaacaga cagatttaga tatttatgga ccggtcggta taaaaagttt tgttatgact
    13981 ggcttaagga catcaggttc acgtttacct tatcgaattc attttcacga gtttgatgaa
    14041 tcatcactcg gaaaaattat ggaaacagat aaatttaccg tttatgcaga aaagctagat
    14101 catactatct tttgtatggg atatcgtgtt gtgcaaaaag accttgaggg cactttagat
    14161 gctgaagcat tgaaactagc aggtgttcca tttggtcccc tttttgggaa agtcaaaaat
    14221 ggtgagaatg ttaccttaga agatggtaga gaaattattg ctaaagatta tatttctgaa
    14281 ccgaaaaagg gtaaagtaat cactattttg ggagatacta gaaaaacaga tgcaagtatt
    14341 aggttagctt tgggcgctga cgttttagtg cacgaatcta cttatggtaa gggtgatgag
    14401 cgaattgcta aatcgcatgg gcattcaacc aatatgcaag cagctgatat cgctaaacaa
    14461 gcaaatgcta aacgactatt attaaatcat gttagtgctc gttttatggg gcgagattgt
    14521 tggcaaatgg aagaagatgc aaagacgatt ttttcaaata cccatcttgt tagagatttg
    14581 gaagaagtag gtatataaaa tggcaagaac gatactgatt acaggagctt ctggtggttt
    14641 agcacaggca attattaacc aattacctca agacgaccat ttaattgtca cagggagatc
    14701 aagagaaaaa cttgaaaagc tttatggaaa gagaccaaac accttatgtt tatcactaga
    14761 cataactaat gacaatgctg taacgaatat gattgaaaaa atttatgttg aatttggtca
    14821 aattgatatt ttaattaata atgctggatt tggttctttt aaagaatttt gggattatag
    14881 tgatgaagaa gtcaaagaca tgtttgctgt gaatacattt gcaacgatgt ctatagctcg
    14941 tcagatagga cataagatgt ctcttgtaaa atctggtcat atcgttaata ttgcaagcat
    15001 ggctggcctc atagcaacaa gtaaagctag cgtttatggg gctagtaagt ttgctgtcgt
    15061 tggatttttg aatgctcttc gtttagaatt agcagaaaaa aatgtttatg tgacaagtgt
    15121 aaaccctgga ccaatcaaaa caggattttt tgctcaggct gatccaagtg gtgattattt
    15181 agcaagtata ggtcgttttg ttctaacacc agaaaaagta gctaaaaaag tagtttctat
    15241 tcttggaaaa aataagagag aattaaatct tccttttata ttagcttttg cgcataaata
    15301 ttatagttta tttccaaaaa cagctgatta ttttgccaga aaggtattca attataaatg
    15361 atatctgcta agtatagttg ggtcctcaat aaccaaaagc cagacgctgg cttttttgag
    15421 gcttcaaaaa aagaaaagat aagtgaagcg gtggcttcct taatttattc gcgaggtatt
    15481 aagacatcag cggaattaca tcatttttta caaactaatt tggaaaatct ccatgatcct
    15541 tatttgttaa atgatatgga taaagctgtt aatcgtatta gacgagctat tgaaaataat
    15601 gaaactattt tagtttatgg agactatgat gctgatggaa tgacatcagc atcaatcatg
    15661 aaagaagcat tagatatgat gggagctgaa gtccaggttt atctcccaaa tcgctttaca
    15721 gatggttacg gtcccaatca aagtgtttat aaatatttta ttgaacaaca agatgtttca
    15781 ttaatcatta cggttgataa tggtgtggca gggcatgaag caattgcata tgctcaaaat
    15841 caaggtgtgg acgtggtagt tactgatcat cacagtatgc cagcagattt accttgtgct
    15901 tatgctatta ttcacccaga acatcctgat gctaattatc catttcctta tttagcaggg
    15961 tgtggtgttg ctttcaaagt agcttgtgct ctcttagaaa caatcccgac agaaatgttg
    16021 gatttggttg ctattgggac gattgcagat atggttagcc taactgatga aaatcgtatc
    16081 atggtaaaag ctggacttga agttatgaaa gatagtgaaa gaataggctt acaggagcta
    16141 atatctttat caaatattga tttaaaaaca ctaaatgaag aaactattgg atttaaaatt
    16201 gctccacaat taaacgcttt aggaagactt gatgacccta atccagctat tgaattacta
    16261 acagggtttg atgatgaaga atctcaagca atagcccaaa tgattgatca aaaaaatgaa
    16321 gagcgcaaag aaatcgttca aactatcttt gaccaagcta tgcaaatgct tgatcaaaca
    16381 aagcctgtac aagttttagc aaaagaaaat tggcatccag gggttttagg tattgtggcg
    16441 ggtcgtattt tagagagaac tggacaacct gttattgttt tgaatattga agatggtatt
    16501 gctaaaggaa gtgccagaag tgtagaagca cttgatattt ttcaagcttt tgaccagcat
    16561 cgagaattat tcattgcttt tggaggtcac agcggagcag caggcatgac tttagaggaa
    16621 agtaaggtag gtgatctttc tcaagtttta tgtgactata tctctaaaaa acaattggac
    16681 atgagtcaga aaaaaactct gacaattgat agtgagctac gttttgacga acttagttta
    16741 gacactgtta gagattttga aaagttagcg ccatttggaa tggataataa aaaaccagtc
    16801 tttttgttaa aagattttaa agtgagtcaa gccagagtca tggggcaaaa cggtgctcat
    16861 ctgaaattaa aactagaaca agatggacaa gcacttgatt tggtagcatt taatatgggg
    16921 agtcaactac aagagtttca acaagcacaa catttggagt tagctgtcac tttatctgtt
    16981 aatcaatgga atggtgccac aaccctacaa ttaatgttgg aggatgcgcg tgtagatggt
    17041 attcaattat ttgatattag aagcaaagca agtagcttac ctcatggagt gccaatcctg
    17101 tctcaagaag agcagagcaa agaagttatt cttttgactg ttcccgatca tccacaagaa
    17161 ttaaaacaaa tgactcaagg taaacagttt gatgctatct attttaaaaa tgagatacca
    17221 aaaaattatt ttatatctgg ttatggtaca cgtgatcaat ttgctagttt atataaaaca
    17281 atatatcaat tcccagaatt tgacgttcgt tataagttaa aagagctcag ttcctacctt
    17341 catattcctg atattttatt aattaaaatg attcaaattt ttgaagaact acattttgtt
    17401 acgattacag aggggattat gacagtcaat aaagaagcag aaaagcgaga tatctctgag
    17461 agtcaaattt atcaagaatt aaaagagaca gttaagtttc aagaattaat ggctttaggg
    17521 acacctaaag aaatatatga ttttatgatg agttgataat atactaaaga agccactttt
    17581 taagtggttt ttttggtata taaataagtt atttcaataa taatattgca aatattatta
    17641 aaatataata aaattaagaa aataatggaa tttttgataa agaatattaa aggtgaggat
    17701 aaaatctatg agacctatcg ttagaaaaaa gatgtacaaa aaaggtaaat ttttggtcat
    17761 tgcaggtatc gttactattt taggtggtag tgcaattctt ggtcaagatg tcaaggcaga
    17821 acaggcagaa gcagtaactt caacgatctc agagaaaact gatagcagtc agactatttc
    17881 ggatatttcc aagttaacgt tacctgttaa ttcctcggag gctatgaaaa atagtgcaga
    17941 gcctttaata aaaacaggct ttgctacatc tgtttcatct aatcctagag agatagcagc
    18001 taccccagtc aaaacctttg atgctagttc aaaagttgtt gtaaaagcta gtactgctga
    18061 gcactcagca aatcaaacaa attctaatgt taatcaagta gctaatgata gtgaagttat
    18121 tactcaacaa aattcaacaa agcaattacc aacagtgact tactctgctc atgttcaaga
    18181 tattggatgg caaaagtcgg ttgataatgc aacagtttca ggaacagtag gacaggaaaa
    18241 gcaggttgag gcaataaaat tgtctattaa agcccctgaa ggaataacag gaaaacttag
    18301 ctataaaact tatgttaaag gacaaggttg gcaaccttca gtagagagtg gtcaagttag
    18361 tggtactgtt ggtcagagta gacctattga agcactaagc attaacctta ctgataattt
    18421 acaaaaatta tatgatgtct attatcgtgt tcatgttcaa gatatcgggt ggatggcttg
    18481 ggctaaaaat ggagcttacg ctgggacttt gggaatgtca aagcgccttg aagcttacaa
    18541 agtaaagttt acccttaaag gacaatctgt tttaacacca actatactta aggaagagag
    18601 acctgtctta aattatcagg ttaaggtagg ccaaaatggt tggcaatcta ataaacttga
    18661 aggacaaatg gcgggaactt taggagaatc caaagcccta gatggagtaa aattcactct
    18721 tagcaccttg aagtatgggg acattctcta tcgtactcat gtacaagata aaggttgggg
    18781 agaaatttta ggtagtcaaa caagtaagga ttatcaagga aaaagactag aagctattca
    18841 attggactta agtgggaatt taaaacaaaa ttatgatatt tattatcgcg ctcacgtcca
    18901 agataaaggt tggatggctt ggcaaaaaaa taatagtgta gcaggtacag tcggagaatc
    18961 gaaacgttta gaggctttgg aagtaaaact agtagctaaa gcttcagatt ttagttccca
    19021 aacaaatcat tcattcttag aaatgaagca tcctggatta agttatcaaa cttatcttca
    19081 aaaagatggt tggaaaccaa cggttttgga aggccaatta ggaggtagta ttggactttc
    19141 aaaatctata aaagccatca aacttaactt aggtagtaca gctctaggaa atatagaata
    19201 tagaactttt cttaatggtt caggttggca aactgttgtt aattcaggga gagaaagtaa
    19261 tgtaccaaat gaatctcaac aagtagaagc tattcagatg cgattgacag gtctgctatc
    19321 gaaaacctat agtgtgatgt atcgagttca catgcaagat tatggttggc aggattggac
    19381 ttataacaat gacatcgctg gtagtactaa ccaaggaaaa cgaattgaag caatcgaaat
    19441 taaattagtt gagactgcta aaatgcctat aactgtccaa acgacctata agggaactgg
    19501 aaactataat gtacgagtga caaatgttat tagtcctggg aatttaaaaa ttgctatttg
    19561 gtcagataaa aacaatcagg atgaccttaa gtggtatacc gtcacaccca aaaatcatga
    19621 agctagattg acatttaatg ttacaaatca tagagacaat ggtaaatact ttatccatgt
    19681 ttatcaggaa aatgcttcta atcaaaaaca attattggta aaaacttctc tacaagtcgc
    19741 acattcaaat tataatactc cttactttag ccaacgtgat ggaaggtggg caagtcgaag
    19801 atatggtatg gcaactttag gagaaactgg ttgtgtacca acttctctag ctatgatttt
    19861 gtcttctttg aaaggagaaa ctgttttacc aacgcaaatt gcagattacc tttaccataa
    19921 gacagttgaa tttaatcgtg gtgttcaagg aacaacttct agaggaatct taaaagcagc
    19981 caaagaatgg ggtgtaacag caactgcatt aggaactcaa gctaaccttg tacaagcgtt
    20041 gaaggatggt tatcatgtcc tagcggcagt acaaaataat gtttttgttc ttcatggaag
    20101 tcatgaaatc gttctcaaag gatataataa cggactgaca catgtctctg acccttacac
    20161 accgtccttg tctggttggt atccaatttc acaactttgg aaagaacaga gctattatag
    20221 tgaggataga attgatattg gagcaccttt tgtcaaggta acagatgcct aatttttaaa
    20281 accatatgca aaactagcat atggttttaa agtttccagt actattttat ttaattttat
    20341 gatataatga agagtaaaac attttagggg tttcccctta gaaagacaag tgaaatggat
    20401 ttaaataatt acatcgcttc aattgaaaac tatcctcaag aaggaattac tttccgtgat
    20461 atctccccat taatggctga tggtaaagca tacagctatg ctgttcgtga aattgtacaa
    20521 tatgctgctg ataaagatat tgatatgatt gttggaccag aagctcgtgg ctttattgtt
    20581 ggttgcccag ttgcttatgc tcttggtatt ggttttgctc cagtccgtaa accaggtaaa
    20641 ttaccacgcg aagttatttc agctgactat gaaaaagaat atggtcttga tacattgaca
    20701 atgcacgcag atgctattaa accaggtcaa cgtgttttaa ttgttgatga ccttttagct
    20761 actggtggaa cagtaaaagc gacaattgaa atgattgaaa aacttggtgg cgttgtcgca
    20821 ggctgtgctt tcctagttga acttgatgga ttaaatggtc gtaaagctat tgaaggttat
    20881 gatactaagg tattgatgaa ctttccagga taaggcctat tcaagataga tgaatgataa
    20941 gggcgcctct aagtgctatg ataatagttg agaggtgctt tttgttacac atttcataat
    21001 tttaggacga tagagaggat ttgaaaggtt atgacttatt tggaacaata tcagtcaggg
    21061 caattgactc taccaagtgc tttatttttt cactttaaat ctatttttaa gacagcagac
    21121 gattttttag tttggcaatt tttctattta caaaatacaa ctaatttatc cgatctaaca
    21181 ccgagtcgta ttgcgacatc acttgataaa acagtggcag atatcaatcg ttctatatcg
    21241 aatttaacaa gtcaaggttt actagatgtc aaaacaattg agttgaatca cgaaatcgag
    21301 attatctttg atacaagccc tgtgtttgct aaattagata aacttttcga agaggataat
    21361 caagtaataa tagacaataa aaccgctgat tcgaatcgtc tgaaagattt agtaggagac
    21421 ttcgaacgtg aattaggtcg cttattaagt ccttttgaat tagaagattt acaaaagaca
    21481 ctgcaagaag atcagacaga tcctgatatt gttagggcag ctcttagaga agcggttttc
    21541 aatgggaaaa caagttggaa ttatatcaat gctatccttc gcaactggcg gcgtgaagga
    21601 ttgacgacac ttcgacaaat tgaagagaga aaacaagcac gtgaagataa ccaaatgaaa
    21661 gatttagcga tttcggatga ttttaaaaat gcaatgaatt tatgggaata agtttactag
    21721 gaggagacag aatggttgat acattaggtc tgctcctttt cttttgatgt taatgatgtt
    21781 atacttttaa cagagaaaaa ctgaaggaaa tacaatggat ttacaattat caaaacgatt
    21841 acaaaaagtt gcaaactatg ttccaaaagg agctcggtta ttagatgtag gaagtgatca
    21901 tgcttactta cctatttttt tgttgcagat gggatactgt gattttgcca ttgctggtga
    21961 agtggtgaat ggaccgtatc agtctgcgtt gaaaaacgtt tcagaacatg gtctcacttc
    22021 aaagatagat gttcgcttag caaatggttt gtcagctttt gaagaagcag ataatattga
    22081 tacgatagct atctgtggaa tgggtggtcg tttgattgct gatattttga ataacgatat
    22141 agacaaactt cagcacgtta aaacactagt cttacaacct aataatcggg aagatgattt
    22201 aagaaaatgg ttagctgcaa atgattttga aatagtagca gaagatatct tgactgaaaa
    22261 tgacaaatgc tatgaaattt tagttgttaa gcatggtcat atgaatttaa ctgctaaaga
    22321 attgcgattt ggtccatttt tattatccaa taacacaaca gtttttaaag aaaaatggca
    22381 aaatgagctt aataaattaa cttttgcttt aaactctatt cccaatagta aaatggaaga
    22441 acgtgctatt ttggaagata aaatacagga tataaaggag gttcttgatg aaagctagag
    22501 aacttattga tgtatatgaa acttattgtc cacaggaatt atctatggaa ggagatattt
    22561 caggtcttca aattggatct ttagataaag aaataaagac agtcatggta gctttagatg
    22621 ttcgagagac tacagtagct gaagcaattg aacgtcaagt agatcttctt attgtaaagc
    22681 atgctcctat ttttcgtcca ttaaaggatt tggtagcaac accgcaaaat aagatttata
    22741 tagaccttct taaatcagac attgctgttt atgtgagcca tactaatata gatatcgttc
    22801 ctaatggact taacgactgg ttttgtgaat tattagatat tcaatatccc gatatattat
    22861 ctgagacaag taatggttat ggtattggtc gcattggtga tgttagaccc cagagttttg
    22921 aattttttgc atggaaaatt aaagatgtgt ttggtttaga tagcgttcgt ttagtttcat
    22981 atgataaatc taatcctgaa attcaacgag ttgctatttg tgggggtagt gggcaaagtt
    23041 tctacaaaga agctattgca aaaggagcag atgtttttgt aacgggagat atttattatc
    23101 atactgcaca agaaatgatc acaaatggtt tgcttgcgat tgacccaggc catcatattg
    23161 aggttttatt tgtttctaaa atagctacta tgattgagca gtggaaactt gaaaaaggct
    23221 gggatatttc agttttagag agtaaagctc caacaaatcc cttttaccat atgtaggagc
    23281 tattatgaaa aagatagcaa ttattggtgc aggagcagtc ggagcgacac tagcctatta
    23341 cttatcaaaa gaaaaggata ttcaggttac tgtttttgat tatggagttg gacaagcaac
    23401 taaggctgct gcggggatta ttagcccatg gttttcaaaa cgtcgtaata aagcctggta
    23461 tcgcatggcg aggttggggg ctgattttta ttcaaagcta gtcacggatt tacaaaaaga
    23521 tggttttgaa acaaaatttt atcagcaaac tggtgttttc ttattaaaaa aggacgagag
    23581 tcagttagag tcattgtttg cactagcaga taaacgtcga ctagaatctc ctctaatagg
    23641 tgatttacaa atactaaata aaagtgaagc aaatactcac tttcctgagt tagatggcta
    23701 tgaacagtta ctttatgcga gtggaggagc gcgagtagaa ggggcggact taacacgaat
    23761 attgttagaa gcaagtggtg ttaatgtcat caaggatgaa gttcacttta cgataactga
    23821 taatggcttt cgtgttcaag gaattgattt tgataaactt gttttagcga gtggagcctg
    23881 gctagctaag atattagatg agcacaacta tcaggtagat attcgccctc aaaaagggca
    23941 attgcgagac tattatttta gcaatattaa tacaggaaaa tatcctgttg taatgccaga
    24001 aggcgagcta gatattatcc catttgataa tggtaaagtc tctgtaggtg caagtcacga
    24061 aaatgatatg gcttttgatt taaacattga ttttaaagtc ttagacaagt ttgaagagca
    24121 agctattggc tattttccgc aattaaaaaa agcagatacg acttctgaac gtgttggaat
    24181 tcgtgcctat acaagtgatt tttctccatt ttttggccct gtcccttgta tggagggtgc
    24241 atatgctgca agtggattag gatcaactgg cttaacagtg ggcccattaa ttggttatga
    24301 attatgtcaa ttaatcttaa ataaagaaaa tcagctaaat ttagaggatt acgatattac
    24361 gaaatacgtg aaaaagatta gtagatgata gcgtttaaaa aaagtgttat aatatgtagg
    24421 attaatataa ataaggagac cttactatga aaggtattat tttagcggga ggttcaggta
    24481 ctcgcttgta cccattaact cgtgctgcat caaaacaatt gatgccaatt tatgataaac
    24541 caatgattta ttatccattg tcagttttga tgttggcagg aataaaagaa atactgatta
    24601 tttctactcc acaagatttg cctcgctttg aagatatgct tggagatggt tcagaactag
    24661 gaatctctct ttcttatgct gaacaaccaa gcccagatgg gttagcacag gccttcatta
    24721 tcggtgaaga atttattggc gatgatcacg ttgccttagt attaggtgat aatatttacc
    24781 atggaccagg tttatctgca atgctacaac gtgcagctag taaagaaagt ggagcaaccg
    24841 tctttggcta tcaagtaaaa gatccagaac gttttggtgt tgtcgagttt gatactgata
    24901 tgaatgccat ttctattgaa gaaaaaccag cgcaacctaa gtctaattat gctgtcacag
    24961 gtctttattt ctatgataac gatgtggttg aaatagctaa aaatattaag ccaagtcctc
    25021 gtggagaact tgaaattact gatgttaaca aagcctacct tgatagaggt gatttatcag
    25081 ttgagttaat gggacgtgga tttgcgtggt tagacactgg aactcacgaa agtcttcttg
    25141 aagcagcaca atatattgaa acagtacaac gcatgcaaaa cgttcaagtt gctaaccttg
    25201 aggaaattgc ttaccgcatg ggctacatca ctcgtgaaca agtccttgag ttggcacaac
    25261 cacttaagaa aaatgagtat ggtcaatacc tacttcgttt gattggagaa gcctaatgac
    25321 tgaacaattt tttgataaag aattaacttg tcgcccaatt gaagccattc ctggattgtt
    25381 ggaattcgat attaccgttc gtggagacaa ccgtggatgg ttcaaagaga actttcaaaa
    25441 agaaaaaatg ataccgcttg gtttcccaga aagcttcttt gaggcagaca aactacaaaa
    25501 taatatttcg tttaacaaaa aaaatacttt gcgaggtctc catgcagagc cttgggataa
    25561 atatgtttcg atcgctgatg aaggacgtgt gatcggtact tgggttgacc tccgtgaagg
    25621 tgacagtttt ggtaacgttt accaaacgat tatcgatgcc tcaaaaggta tttttgtccc
    25681 acgcggcgtt gctaatggtt tccaagttct ttcagataaa gcagcttata cttatctcgt
    25741 taacgattat tgggcacttg aactcaaacc aaaatatgct ttcgttaact atgcagatcc
    25801 aaatctaggc attcagtggg aaaaccttga agaagcagaa gtctcagaag cagataagaa
    25861 tcacccactt ctcaaagatg taaaaccttt gaagaaggaa gatttgtaat tttcaacttc
    25921 ttaaaaggca ttaaccttag caagtaaaaa cgaattagca atgttgtttg acgcaagggt
    25981 gaaaggaaac atgcaaaagg cctgttccct cagggatttg aaatattgat ttcttaggca
    26041 tgacattcca tagaactact tctgacagaa cagtatcctt caaacagcaa aaagaaaaaa
    26101 tttttcggag aaaaaatgac tgaatttaaa aacattatcg taactggtgg agctggtttc
    26161 atcggatcaa actttgtaca ctatgtttac aataaccacc cagatgttca tgtaactgtc
    26221 cttgataaat tgacatatgc aggtaaccgt gctaacattg aagccattct tggtgatcgt
    26281 gttgagttag ttgttggtga catcgctgac gctgaattgg tagacaaatt ggctgccaaa
    26341 gcggatgcta ttgttcacta tgcggctgag agtcacaacg ataactcatt ggaagatcca
    26401 agtccattta tccatacaaa ctttatcgga acttacactt tgcttgaagc agctcgtaaa
    26461 tacgatatcc gtttccacca cgtgtcaact gatgaagttt atggagatct tccacttcgt
    26521 gaagaccttc caggaaatgg tgaaggacca ggtgaaaaat tcactgctga aacaaaatac
    26581 aatccatcat caccttactc atcaactaag gcagcttctg accttatcgt taaggcatgg
    26641 gtacgttcct tcggcgtaaa agcgaccatt tcgaactgtt caaataacta tggaccatac
    26701 caacacatcg aaaaattcat tccacgtcaa attacaaata tcttggcagg aatcaaacca
    26761 aaactttatg gtgaaggtaa aaacgtccgt gactggatcc acacaaatga ccattcaaca
    26821 ggtgtctggg ctattttgac taagggtcgt attggtgaaa cataccttat tggtgccgac
    26881 ggtgagaaaa acaacaagga ggttcttgaa cttatccttg agaaaatggg tcaaccaaaa
    26941 gacgcttacg atcacgtaac tgaccgtgct ggtcatgatc ttcgttacgc tatcgattct
    27001 acaaaattgc gtgaagaact cggctgggaa ccacaattta caaacttttc agaaggtttg
    27061 gaagaaacta ttaaatggta tacagaaaat gagacatggt ggaaagcaga aaaagatgct
    27121 gtagaagcca agtatgctaa aactcaagaa gtcattaagt aattgaacca aaaaacctat
    27181 cattttgatg ataggttttt tatgtacggc tagttagata gctaattggt ctgtttttgt
    27241 catggtttga ggttggtaat agacattttg atagtcatta ccaacttttt gtactaggta
    27301 aagtcccgct ttattcattt tgaattgatt gttaatgaga ctctcttgat tatcgtgttt
    27361 gataacagcc caagtttgac tgtttttatc atagataact tgttgttttg aactattttc
    27421 tagtaattct acttctttgc tgttggctaa tttatcaaaa cttgtgcgat caatgtttgg
    27481 aaccatcatg taaccatagc tatcaccttt gttgtcatgc ttttggctta tagtgataaa
    27541 aggattgcta acgattgagg tatttttaga agtacgatta atgctgttcc aagtacctgt
    27601 ttgctctttg cgttcaatat caatagtgct atttttaaag aagatataac caatattgcg
    27661 accaggttct tttgattcta aaaagacact ttttgtatct gtaaattgtt gagaacttgc
    27721 ttgttttaaa tcaatagttt ttccattgac gtatgtagta taaggtgttt tagaatcgtc
    27781 ttttcgttga tcaattgttg tagaaacatt tccaatgccg ttagtattct tgatgttgct
    27841 acctaaaaag acaatcttat catttaagat aacccaacct ttttgtgctg ttaaggtgcg
    27901 atcccagtta gtaaaatcca tagcggcaag agcaaagtga tcattaagtt tgacggaacc
    27961 aacaaagtca gatgttcctg taacttgacc ggttttttct ttagcgtctt tgctatgttt
    28021 gctcatgaat tccttagtgg tatcttcacg cttagcatct ttttcagttg ttccagccat
    28081 tttataagga ttgacggttg gccaaaaatg attactataa tgagattgat cactattata
    28141 aatatagaac ataccatctc cggtatacca accacgtgta ttttcatcat tcattccttc
    28201 atagttgagg gtacgtttag aatgtaatga aagcgcgaaa ccaaagtctt tcttggcatt
    28261 ataataagct aagcggtcca tgctattaaa ggttgataaa ttacttttta aaggtttagt
    28321 agcgactgta ctgtcattaa gcagcttatt catgttggca atatcggaat acgatttgag
    28381 gttattgaag acattgtaga atttatttga agtgataatc gttttaatag ttgatttgag
    28441 gtctaagttt cgctcttcat tagacatgtt agccaatctg aggaaacctc tgagaacttc
    28501 aactgcagcc gcatgcgaag aagctgcctc tctactaatt gagcgtccac gactcatatc
    28561 cattaactca ccttttacaa ttaaaggtaa aaatgattga ttaatccatt tataaaccat
    28621 atcaagttct tgattagaga ttttatagtc agtttcttga atgataggca gcaattgtgt
    28681 caaaccatct atcagaacat taccataggc gccagtataa gcaacatttg tatggtcgat
    28741 gtaagaaccg tcagcataga agccttcagc tttagtagca gtagtaaaaa gattttttag
    28801 agaatgagaa gttttttcga taatagtatt gtctttacga agtaaacctt caatgatttt
    28861 aacgcgcccc atatctacta gattaccacc aagggcttta aatggattga caagcgtttt
    28921 acggaaatat tctgcatcag gaacaaagtg ttcaattggg tcggtataag tttttatttc
    28981 agcgtcagtg aagtagttat tcatgagaga tagggtacct gtaattgagc gaggaacacc
    29041 gatttcaaaa tcccaccaat tggcagagcc ttctatatct ttattaacat tgtagaagtt
    29101 ttgatgaagc caagccagac tctcttttac agtacgaata gctttttcat ttttgtaaat
    29161 agtagagtgt ggattggtga tttgtttagc taaatcttcc aaacgacgat aagtagcggt
    29221 taactgtgct gaattattga gattatctaa atctttccaa aggaaagtgc gattagaatc
    29281 cagtttgata gcttcgatgt ttttggcgtt agtttcatct aatttctgat taagcttttg
    29341 catgttcgaa tcattagtat cgtaaacata attaccaata gtcacgtcgt tccatttgtc
    29401 gaggagtttt gtaaagttat cttctgttga agctgtaact gataacggta cttctttaat
    29461 tattttgcca gatttatcag atattttaac agttgttgaa ccagtagcgt ttggatacaa
    29521 tattccacca acaattttcc caagagacgg attggttaga gtgtattggt agtcagcttt
    29581 attaaaaacg taatttttgt ttaaagccgt attaacgctt ttttcaattt gtgttgtgac
    29641 gggttgtgga tgctctgagt ctttagggcc ttttgctttc attgatatat tatcaaaagt
    29701 aacaaaacca gttccttttt cataataaag ttcaagtgtc acctcggaga catttaactt
    29761 tgggttatat atctttgtta aggtatgttt gttagtagta ccgctggtca tctcagaaag
    29821 ccaaagtcgc gtattgttgt cttttttctc cataatacga aggaaagctt gtccagcctt
    29881 gttacttgtt tcaatatcaa acttaacctc atatttttta gtgggatcaa tgttcacttt
    29941 ttgtgttaca gcacctctaa agcctttgtc gctggataga cttacttggc cttgttcggt
    30001 acgttggata ataggtgatt tatcagcagt atttccttta tcaatccaag cagaccattc
    30061 cctagcgctt gtatgtgacc agcttccaga tacagggtta gtttgattaa aatctccgtt
    30121 tttcaccagc tccttatttt gagtttgatt atctactact tgtttcattg tgctatgagt
    30181 ggctgttgca gttgcagttg aagctgtggg gttagacaga tcttgttgtg tggttgtggt
    30241 gctagtctcc ttgacagtgg tactttcagt ctggagaact tcaatattat tagtttgagt
    30301 atctcttatt tgggacgttg aggtagtttt ggtaagctct tcagctttag cttggtaact
    30361 attgttaacc aatattgttc ctaaaataag ggctgaataa agcataatac gatgtttctt
    30421 tttgatttcc atttttactc cttagggttt aaaattgtaa accggttttt cctaattata
    30481 gcatgcttta aaggtttatg ataaagaacc tttaaaatgt catcaaagtg aaaagtaaat
    30541 aactggtaaa caaaataatt tgtttaatca tttgtgtgct actttgatag taaattgcct
    30601 aaataaaggc tattttttgt tataatatga gaatgagagg agtttgaaat gactaaatta
    30661 gcaacaattt gttacattga caatggcaag gaattacttc tattgcatcg caacaaaaaa
    30721 gagaatgatg ttcatgaggg aaagtggatc tcggtaggcg gtaaactgga ggcaggtgaa
    30781 actccggatg aatgtgctaa gcgtgaaatt ctagaagaaa cccatttaac agtaaaaaaa
    30841 atggatttta aaggagtcat cacttttcct gaatttacgc caggtcatga ttggtatacc
    30901 tatgtcttta aagtaacaga ttatgaagga gagttaattt cagatgatga atcacgagaa
    30961 ggaaccttag aatgggtacc atatgatcaa gttctctcta agccaacgtg gcaaggagat
    31021 tacgaaattt ttaaatggat tttagaagat gttcctttct tttctgctaa gtttgtttat
    31081 gatgaacacc aaaatttaat tgaaaagacg gttaattttt atgaaaaata gagattttag
    31141 ggttagagac cagctcgaaa cattcggagt cagagtttct gctttaatca tagaaaatca
    31201 aaaactgcta ttaatatatg ctcctcatct ctataaatac tatttgccag gtggggcgct
    31261 tcaagttgga gaagatagca ataaggctgt tgcgagagaa gtacttgagg aaataggtct
    31321 tcacagccaa gttggtgatt tagcctatat tgttgagaat cagtttaata ttaagagaca
    31381 tcattatcac tcagttgaat ttttatattt tgttaatctc ttgggtcagg cgccagaatc
    31441 tatcaaagaa ggaacgcata agcgacattt tgtatggctt ccaataaaag aattaaca
//
LOCUS       DATRSG010000014        31225 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRSG010000014 DATRSG010000000
VERSION     DATRSG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31225
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.014"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..314)
                     /gene="dhaS"
                     /locus_tag="VBD89_000662"
     CDS             complement(<1..314)
                     /gene="dhaS"
                     /locus_tag="VBD89_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO7135213.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELLTFFKMNQEFYIKLFQIEDQ
                     ND"
     gene            488..1477
                     /gene="dhaK"
                     /locus_tag="VBD89_000663"
     CDS             488..1477
                     /gene="dhaK"
                     /locus_tag="VBD89_000663"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO7135214.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            1515..2093
                     /gene="dhaL"
                     /locus_tag="VBD89_000664"
     CDS             1515..2093
                     /gene="dhaL"
                     /locus_tag="VBD89_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO7135215.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            2093..2467
                     /locus_tag="VBD89_000665"
     CDS             2093..2467
                     /locus_tag="VBD89_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO7135216.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            2477..3190
                     /locus_tag="VBD89_000666"
     CDS             2477..3190
                     /locus_tag="VBD89_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7135217.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     DPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(3455..3859)
                     /locus_tag="VBD89_000667"
     CDS             complement(3455..3859)
                     /locus_tag="VBD89_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7135218.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(3998..4712)
                     /locus_tag="VBD89_000668"
                     /pseudo
     CDS             complement(3998..4712)
                     /locus_tag="VBD89_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(4805..5914)
                     /locus_tag="VBD89_000669"
     CDS             complement(4805..5914)
                     /locus_tag="VBD89_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO7135219.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            complement(5911..6162)
                     /locus_tag="VBD89_000670"
     CDS             complement(5911..6162)
                     /locus_tag="VBD89_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135220.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            complement(6185..6919)
                     /locus_tag="VBD89_000671"
     CDS             complement(6185..6919)
                     /locus_tag="VBD89_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7135221.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            complement(6985..7341)
                     /gene="rsfS"
                     /locus_tag="VBD89_000672"
     CDS             complement(6985..7341)
                     /gene="rsfS"
                     /locus_tag="VBD89_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO7135222.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            complement(7343..7864)
                     /locus_tag="VBD89_000673"
     CDS             complement(7343..7864)
                     /locus_tag="VBD89_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7135223.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            complement(7927..8514)
                     /gene="yqeK"
                     /locus_tag="VBD89_000674"
     CDS             complement(7927..8514)
                     /gene="yqeK"
                     /locus_tag="VBD89_000674"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO7135224.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            complement(8511..9143)
                     /locus_tag="VBD89_000675"
     CDS             complement(8511..9143)
                     /locus_tag="VBD89_000675"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO7135225.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     CISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            complement(9273..9590)
                     /gene="yhbY"
                     /locus_tag="VBD89_000676"
     CDS             complement(9273..9590)
                     /gene="yhbY"
                     /locus_tag="VBD89_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO7135226.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIRVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            complement(9683..10801)
                     /gene="yqeH"
                     /locus_tag="VBD89_000677"
     CDS             complement(9683..10801)
                     /gene="yqeH"
                     /locus_tag="VBD89_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO7135227.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            complement(10801..11361)
                     /locus_tag="VBD89_000678"
     CDS             complement(10801..11361)
                     /locus_tag="VBD89_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO7135228.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASYRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            complement(11445..12359)
                     /locus_tag="VBD89_000679"
     CDS             complement(11445..12359)
                     /locus_tag="VBD89_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO7135229.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            complement(12489..13931)
                     /gene="gatB"
                     /locus_tag="VBD89_000680"
     CDS             complement(12489..13931)
                     /gene="gatB"
                     /locus_tag="VBD89_000680"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO7135230.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            complement(13931..15397)
                     /gene="gatA"
                     /locus_tag="VBD89_000681"
     CDS             complement(13931..15397)
                     /gene="gatA"
                     /locus_tag="VBD89_000681"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO7135231.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            complement(15397..15699)
                     /gene="gatC"
                     /locus_tag="VBD89_000682"
     CDS             complement(15397..15699)
                     /gene="gatC"
                     /locus_tag="VBD89_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO7135232.1"
                     /translation="MKISEEEVRHVAHLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            complement(15837..18482)
                     /locus_tag="VBD89_000683"
     CDS             complement(15837..18482)
                     /locus_tag="VBD89_000683"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO7135233.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            complement(18495..19325)
                     /locus_tag="VBD89_000684"
     CDS             complement(18495..19325)
                     /locus_tag="VBD89_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO7135234.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            complement(19336..19986)
                     /locus_tag="VBD89_000685"
     CDS             complement(19336..19986)
                     /locus_tag="VBD89_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135235.1"
                     /translation="MYKEGDLIQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALR
                     SDLAVLSMLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYD
                     VIVHIFMEDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTT
                     VLENESLFAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            20154..21119
                     /locus_tag="VBD89_000686"
     CDS             20154..21119
                     /locus_tag="VBD89_000686"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO7135236.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLYIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTTSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(21166..21714)
                     /locus_tag="VBD89_000687"
     CDS             complement(21166..21714)
                     /locus_tag="VBD89_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7135237.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(21781..22566)
                     /gene="codY"
                     /locus_tag="VBD89_000688"
     CDS             complement(21781..22566)
                     /gene="codY"
                     /locus_tag="VBD89_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO7135238.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(22692..23903)
                     /locus_tag="VBD89_000689"
     CDS             complement(22692..23903)
                     /locus_tag="VBD89_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7135239.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            24170..24622
                     /locus_tag="VBD89_000690"
     CDS             24170..24622
                     /locus_tag="VBD89_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7135240.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(24677..26059)
                     /locus_tag="VBD89_000691"
     CDS             complement(24677..26059)
                     /locus_tag="VBD89_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7135241.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQCYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQEVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            26128..27090
                     /locus_tag="VBD89_000692"
     CDS             26128..27090
                     /locus_tag="VBD89_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO7135242.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(27125..28003)
                     /gene="aroE"
                     /locus_tag="VBD89_000693"
     CDS             complement(27125..28003)
                     /gene="aroE"
                     /locus_tag="VBD89_000693"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO7135243.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALSIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(28101..29015)
                     /locus_tag="VBD89_000694"
     CDS             complement(28101..29015)
                     /locus_tag="VBD89_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7135244.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKEWYDIYRSAGNILP"
     gene            complement(29306..>31225)
                     /gene="recG"
                     /locus_tag="VBD89_000695"
     CDS             complement(29306..>31225)
                     /gene="recG"
                     /locus_tag="VBD89_000695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO7135245.1"
                     /translation="LLYYPFRYEDFKSKSVFDLVDGEKAVITGLVVTPANVQYYGFKR
                     NRLSFKLRQGEAVLNVSFFNQPYLADKIELGQEVAVFGKWDATKSAITGMKVLAQVED
                     DMQPVYRVSQGISQSTLIKAIKSAFEINAHLELKENLPATLLGKYRLMGRSQACLAMH
                     FPKDITEYKQALRRIKFEELFYFQMNLQVLKAENKSETNGLSILYSKHAMETKISSLP
                     FILTNAQKRSLDEILSDMSSGAHMNRLLQGDVGSGKTVIAGLSMYAAYTAGFQSALMV
                     PTEILAEQHYISLQELFPDLSIAILTSGMKAAVKRTVLAAIANGSVDMIVGTHALIQD
                     SVQYHKLGLVITDEQHRFGVKQRRIFREKGENPDVLMMTATPIPRTLAITAFGEMDVS
                     IIDELPAGRKPIITRWVKHEQLGTVLEWVKGELQKDAQVYVISPLIEESEALDLKNAV
                     ALHAELSTYFEGIAKVALVHGRMKNDEKDAIMQDFKDKKSHILVSTTVIEVGVNVPNA
                     TIMIIMDADRFGLSQLHQLRGRVGRGYKQSYAVLVANPKTDSGKKRMTIMTETTDGFV
                     LAESDLKMRGSGEIFGTRQSGIPEFQVADIVEDYPILEEARRVASDIVKDNNWKENTE
                     WALILDNLRQHSDFD"
BASE COUNT         9853 a         6236 c         4952 g        10184 t
ORIGIN      
        1 aaatcatttt gatcttcaat ttgaaataat ttaatataga attcttgatt cattttaaaa
       61 aaagtcaaca actctgataa caactgaaaa cctgagatat agtctaaatt atccgtaact
      121 tgttcgctta attctgtttg gaaaatccat tctaataaag catatttatc aacaaagtga
      181 ttataaaatg tttgacgtct aatacctgca tcttccataa tatcactcac cgatattttg
      241 tcaaaagctt gcgaaataaa taaacgttta aatgacttgg ctattttttt cttagtaata
      301 agcgaagaag tcataatttc tcctttattt tatattgaat tgctgatgat ttattatggt
      361 ttagtgtaac atgatttatg attattgaaa accctaacaa agcggacaaa caagaaaatc
      421 tgtccattcc agaaaaggct tacaaatttt ataataaaag agaattaaac ataaaaggag
      481 tttccaaatg aaaaaaattc tcaatcaacc aactgatgtt gttactgaaa tgctagatgg
      541 attagcatat gttcataatg atttagtaca tcgcatagaa ggttttgata tcattgctcg
      601 caatgaagag aagtcaggaa aagtggctct catttctggc ggaggaagtg gacatgagcc
      661 ttcacatgct ggtttcgtag gcgaaggaat gttatctgca gctgtgtgtg gtgcagtatt
      721 tacttctcca actcctgacc aagttcttga agcaattaaa gaagctgatg agggggctgg
      781 agttttcatg gttatcaaaa attattcagg ggatatcatg aactttgaaa tggcacaaga
      841 tatggctgaa atggaaggca tagaagttgc aagtgtggtc gttgatgatg atattgcagt
      901 cgaagacagt ctttacacac aaggaaaacg tggtgtagct ggtacgatac tcgtccataa
      961 aattctagga catgctgctc gtcatggcaa atcacttcag gaaatcaaag ctattgctga
     1021 tgaattagta ccaaacattc acactgttgg acttgcacta agcggggcta ctgttcctga
     1081 agtaggcaag ccaggatttg ttcttgcaga agatgaaatt gaatttggta tcggtattca
     1141 tggtgaacca ggttatcgta aagaaaaaat gcaaccttca aaggctttag cgacagaatt
     1201 agttgataag ttaattgaat cgttcgatgc taaatctggc gaaaaatatg gtgtccttat
     1261 taacggtatg ggtgcaaccc cacttatgga acaatatgtc tttgctaacg atgttgccaa
     1321 actccttgag gataaaggca ttgaagtgaa ttacaaaaaa cttggtaact atatgacttc
     1381 aattgatatg gctggtttat cactaacctt gattaaactg gaaaatcaag agtggttaga
     1441 agcacttaat agtgacgtta caaccattgc ttggtaatca ttcctgttaa ttagaacaaa
     1501 aggaaggaac tcttatggaa gtaaaaactg caatagaatg gatgcacact tttaaccaaa
     1561 aaattcaatc caacaaggac tatttatccg aactagatac tccaataggt gatggcgacc
     1621 atggtggcaa tatggcgcgt ggtatgactg ctgttattga aaaccttgat aacaatgaat
     1681 ttagtagtgc tgccgatgtt ttcaaaaccg tgtccatgca acttttaagc aaagtaggcg
     1741 gagcatctgg accactctat ggttctgcat ttatgggaat tacaaaagct gaacagagtg
     1801 aaagtaccat ttcagaggca cttggcgcag gacttgaaat gattcaaaaa cgtggtaaag
     1861 ctgaacttaa tgaaaaaaca atggttgatg tttggcacgg tgttattgaa gcgattgaaa
     1921 aaaatgaatt gactgaagac cgtatcgatt ccttagtaga tgctacaaaa gggatgaaag
     1981 caacaaaagg acgcgcttct tatgttggag aacgctcagt aggacacatt gatccaggat
     2041 cattttcttc aggattatta tttaaagcat tactagaagt tggaggtgtc taatgtctga
     2101 tattggtatt attgttgttt ctcactctaa aaatattgct caaggagttg ttgatcttat
     2161 ttcagaggta gcaaaggatg tctcaataac ttacgtgggt ggaacagaag atggtgagat
     2221 tggtacttct tttgatcaag ttcaacagat tgtagagcaa aatgataaaa agacgttatt
     2281 agctttcttt gatttaggtt cagcaaaaat gaatttagag ttagtagctg atttttcaga
     2341 aaaaaatatt atcattaact ctgtcccagt agtggaggga gcttacaccg ctgctgctct
     2401 tctacaggca ggtgctgacc tggatagtat tcaatcacaa ttagctgaat taactatcaa
     2461 taaataagga gatattatga ctcaatttct tggggaattt ttaggaacat ttattttggt
     2521 tcttcttggg gatggcgtag ttgcaggaaa tgtgctcagt aaaaccaaag aagaaggaac
     2581 tggatggact gctattgttt ttggctgggg aattgcttgt accgttgctg tttatgtttc
     2641 tggattattc agtcccgctc atctaaatcc agcagtcaca ttagcaatgg cttctattgg
     2701 agcaatttct tggggacaag ttatcccctt tatcattgca cagatgttag gagcaatggt
     2761 tgcggcaact attttatggt tacactatta tcctcattgg aaagaaacaa aagatagtgg
     2821 acttatcctt gccagcttct caaccggacc tgctatccgc catacaccat cgaatctcct
     2881 tggtgaaatt atcggtactg ctattcttgt gattacaatt atggcaattg gtcctagtaa
     2941 agttgcagca ggtcttgatc caattatcgt tggtatagtt attttcgcag taggattctc
     3001 tcttggtcca acaactggtt atgctattaa ccctgcacgt gacctagggc ctcgtcttat
     3061 gcatgctatc ttacctattg aaaataaggg taactcagat tggtcatacg cttggattcc
     3121 agttgtaggt ccaattatcg gcggtgtcct tggggctatc ttatacaata ttgctttgac
     3181 tttagtttaa ctaaaaaacc agaaagttaa tactttctgg tttttcatag ttgcagtatg
     3241 ctttcaaaat agaaaaaaca ccaaaatact tagtgttctt tctggagatt ttatgaaaaa
     3301 agtttaggat gtttatagta taaccgccct tacttaacgt ttccttaaaa gctcggttat
     3361 taacattact tcctaaatta ctatttatat tctccaagaa atgactacta ttaagctaaa
     3421 aagcgctatt gacatagcgc tttttagtct agtattattt agtttcagta gtaactccat
     3481 caaaatcttc ttcattttct tcagcttctt ctcttgtggc acggataata ccatctttgt
     3541 gatgagctgg actgtaaatt gtatagagtt ttaacatctc agtttcagaa gtgttaatta
     3601 cgttatgttc agcacctgcc ggaactatta tagcgtctcc atctgcaact tcatattcat
     3661 taccatcaat gacaacctta ccatgaccag cttcaaaacg gaaaaactga tcattttctg
     3721 catgaatctc gctaccaatt tctccaccaa caggcagtgt cattaaaacg agttgacaat
     3781 gttctgcggt atagaggact tggcggaaat ttgtatttcc tagtgtcaat ttttcgatat
     3841 tcccatgaaa tcctttcata acgataccta cctttctatg cctacagtct actctttttg
     3901 ctccttactg tcaaaaattt agataagagt ctgacatttt taataatatc tactgttcct
     3961 tgattcctat catttcattg caaacctaaa atatagccta atgacaatag ctatctatag
     4021 ctttagaaac aaataatgct gttccctggc ctccccaatt atcaatagac ttttggaaac
     4081 gctcatctta gatgtacatt atttcaagac ttgctaataa atcgttagtg cagttgtaaa
     4141 actgagctgt gatataatct tgtaaaatct gaacttgttt ttgaactttc tcatctgtcg
     4201 gagaaagttc tttcaactgt ccaaagtctt taaaaatctg actcatagct tgtgaaatca
     4261 ttgaaaaatc attcttacta tggctatttt caaattcttt gtaactatct gtatcccccc
     4321 cagcgtgttc tagcttcctc ttgaaatact tctaattctt cttgattata agctgtaaaa
     4381 tctgacatat ttttacccct ctggatagac tttgcatgtt caattacttc ttctagtcgc
     4441 tgtttcttta gttccagcca cctaatttga tcaagaagtg cttgattacg atcataattt
     4501 ggactttcca ttatctcttt tattttttta agaggaaatt ccagttcctt aaataacaaa
     4561 atttcttgta acttaatcag tgattcatca tcatagtaac gataaccatt ctctcctaca
     4621 aatgaaggag ataacaaatc tatttcatca taataatgaa gtgtgcgcac actaatacca
     4681 gaaatgtgac ttatttcttt aactgttttc ataataacct ctctgtaaag agtataatgt
     4741 ataacgttaa gtaagggtca agggaaaaat attattttgt gatgattaat ttataaagct
     4801 taaatcaaag gtcactttga ctaacaggtt gataaaaagg gatacgtccc catgtttgct
     4861 ctgctatatt agcatttccc atctgataaa cagaatctgc tcgttgcgtc aaactatccc
     4921 acgtttgtga accaatcctc gtcacgatat ccacagactt cttcacagac tttaaatgct
     4981 gttgaccttt ttgtgtaaaa cctaagacat gaatagcatt tggtatagga ctttccacag
     5041 catttatcaa gatataggtt aataaacgtc gaattcttgc tttggtatac cgtttagttg
     5101 cgactttatc aactaactca tccacagtct ccacataacg aatttgactt tttatacgat
     5161 tggctatctc ttcattcact tgataaattt gtgtcaaatc agaatgcgtc attatctgat
     5221 atttgagtaa actaaaataa tcttgccaaa ctacttgtgg actattcaaa aacaagtcac
     5281 tgttaggcat acctttttct acaaagaaac gatcagattg atgtttacga agacttgttg
     5341 cagaagcaaa tatctctgtt ttttctgatg aatgataacc agctccttga cgttgtatcg
     5401 gatttaatcg aataccattt tggctagctg ctttggtgta ggctaagcct aaaatgtgat
     5461 ttggggtttg tcccgaaaat gtaatccctg caaattcttg ccacatagct tgcatttttt
     5521 gtggatagga aagagaatcc gattgttttt tcaaaaaagc ttccatctcc tcttttttat
     5581 tcacataaat atccccaata cgggcataat caagcatttc ttcggtaccg aagcataagt
     5641 tatccacacc taatcgtgcc aggatagaca cagctccact agcgaaataa tccgctgact
     5701 gtaccgaaac cagaaaaggg agttcaatca ctaaatcagc accattttct agtgccatct
     5761 gggcacgggt ccatttatct acaatcgccg gctcaccacg ttgcataaaa ttacccgaca
     5821 tggctattac ttttatgcct tgagcttgtt ctaaaaggta cttatgccca ttatgaaagg
     5881 gattaaattc ggcaacaatt cctgtaactg tcatacttct ttctctttca attgattatt
     5941 taaagtaaca gcatcaataa tatctaggtg ataagtttta cgaaggtata tgagacgatc
     6001 tgtttctttt tcaaatggtt ttaatgccga taataaatcc aaatgaagtt tagggttcat
     6061 caataattcc aaaataactt tagacttttt ctgacttacc cgacgttcaa taatataaag
     6121 tatcccaaat atcatcagac aagcgtataa gaatattatc atctttttac ctccaaatta
     6181 ttacttattt atgagctaca aaaaaccagc gagcagaagt tgcagttggt tttttatctt
     6241 caaaatctgc atacacctta acatctttaa aacctgcctg ttcaagtaaa atatcataag
     6301 tcaaaatatc ataagtacgc tcttcatgaa cttcatcatg acgtgtaaat cgaccatctt
     6361 cttcttgaac aaaaaatgtt aattcatgga caatagaatg aggtgcatca tcttcataag
     6421 tatcccacac catagcaaag tcatctgcat tttcatgata agaataacca ggaaatactt
     6481 tatctgtttg ataagtcgaa tgaacatcaa aaataaagac gccattttct tctaaggcct
     6541 tatagacttc gataaaaaca tcaccaacct cgacttcatc ttgcatataa caaatagagt
     6601 ctgaatagca cgtgatgaga tcatatttac caacattaga taaatctaac atatttcctt
     6661 caataaactg gatactttgg tgagcagaag ttgcacgttt tttggcaagt ttcagcatat
     6721 cgccactaag atcaagtcct gtaacagcat aaccagcttg tgcaaatcga acagactgaa
     6781 ttcccgtacc acatgctaat tccaaaagtt ttttcttacc ttttggaaaa tgacgaagtg
     6841 aaaaatcagt ccatttatca tataaagtat catccatgac agcatcataa acagctgcaa
     6901 aagtttcata cgttgccata taatagttct caaatctttc ttgatattac atctctcaaa
     6961 aagcagtccc aagactgctt agctttattc tgtcataaag acttcagcat caagcaatgg
     7021 tgcttcatgc caaagttttt caaggttata gtgctgtctc tcatcttcag agaagatatg
     7081 aaccacgacg ctattgaggt caagaagtac ccaaccagct ttactatcgc cttcaaggtg
     7141 gctagcatcc ccaccatttc cttttacttg ttcacggata ttgtctgcga ttgcttctaa
     7201 ctgcctacta ttacttgcac tcataatcac aaagtaatct gcaacactcg ttacaggctg
     7261 taaatctaaa attacaatat cttctgctcg tttttcatcc gcagccttaa cgactagctg
     7321 tagtaagtct ttttctgtca ttttattcct cgtttattaa tttcaataag ctatcttttg
     7381 ctaccattgt cgcaaaacga tgatgccaaa tattctcgta atgtttttta atagaatccc
     7441 cacttaaagt tggtgtatca aatgttaggt gtccaccttg tggaataaag atatcataac
     7501 catattcaaa agcaactttt actgatgtat caaaacagta ttctgtttga agtcccatca
     7561 tgattaattg actaatatgc ttactttgca agtaatcatg taaatgagta tctttaaaac
     7621 aactattgta agttttatca attattttgt gatgcgtcac aagatgtcgc tgttcccaaa
     7681 tttcccactg tggagaacct tgaggcaatt ccttatcatg atgccttatc catatcactt
     7741 cacaattaga acctttggca cttaaaagta aatcatctaa aagctctgca aaatttgtta
     7801 aatgctttgg tttcttatcc ataatacctt gttggatatc aattagtaaa agtgcacaag
     7861 tcatactttc ctccaaaatg accactgcat cgccaaacct atcatgacca ttattcaata
     7921 aaagaattag tcgagataag gtatataagc attataggtt tcaatcgttt taggatagat
     7981 aggctgagcc ttacttgcta aaaacgcaac tgttctagca gtctcataag ctactgcttt
     8041 attaaggtca actttcgcta actcacgagc ttcttcaact cctggaaaat ctcgattgtg
     8101 ttcaatgtaa tctgctacat aaacgatttt atccaatgtg gacatttggg ctgaaccaac
     8161 agtatgtttt gcaatagcag ctaaaatatc ttgatctttg atagctagat cctcttgtat
     8221 tttataaatg ccaacaagac catgccaaat attattaccc cattttttta ggtcgggatc
     8281 tagttggtat ttatcaatta atctaagaaa ttcatcatca gatagttcct tagcataatc
     8341 atgtaataaa gcagctagac ccgctttttc cttgtcataa ccataccgtt ctgctaactc
     8401 tattgcagca cgctctactc ctaacacatg attaaaacgt ttgtcggaca tcatatgacg
     8461 cactttactc aaaagttcag tccgatctaa acctgtgtaa tctttgtagg tcatttgtaa
     8521 agaccttctt tcctgatata atctaatacc tctctaggca gtaaatagtt gggctgacga
     8581 ttacttttaa taaattgacg aatcatactt gatgagatat ccatcaatgg cagatccacc
     8641 caaataacgg gatatgatgt accagcttta tattttggac gttgaacacc aacaaactgc
     8701 accattttaa caagttcatc aatacgatgc cacttaggta gatattccac catatctgcc
     8761 ccaataatga aataataatc aacatctgga tttttttcaa ttagaagctt catcgtatcg
     8821 tatgtatagc tgatacattt acgttcaatt tcaattggtt caatagataa accatcaatg
     8881 ccttctattg ctaattcaag catcttgaga cgatgctgtt catcaattgt ctctttttta
     8941 tcaatatgtg gtggctgaaa ttcaggcatc aacagaactt ggtctaaaca caattgctga
     9001 cgtacttgat ctgcaacaac aaggtgggcg ttatgaacag ggttaaaatt tcctcccata
     9061 attccaattt gttttcggtt agtatctcgc tttttctctt ctaattcaac ttttgtaaat
     9121 ggtgttagca attcgagtgc cataattcct ccatgaacaa gtagtatctc taaaaaaaca
     9181 aacactgatt atcatgatca gctatcatac ggaagtgtaa cttaggtaaa tcgtttttta
     9241 gttctatact ctatttctta atctattgta gtttagtttg attttactgc ttttacttta
     9301 actgaaattt tacgattttc tttgcgcgct gactctttat aaagaatcaa gatacgtcct
     9361 attttaagga cagtatcgca gccaatttca tcttccagaa cttcagcgac atcgtggata
     9421 tcttcatcgg tattttgtaa aagggttact ctgattagct cacgtgcatc gagtgcatta
     9481 cgtacgcttg ttttgatttg atcatttaaa ccatttttcc caatttgaat aatgggtttc
     9541 atagaatgtg cttctgattt taagaaagca cgttgtttag atgttaacat attagtttcc
     9601 tttttatcta ttataggatt atgactgtat catcagctaa attagatgaa aaaccgtcat
     9661 atgttgctgg ttaatttagt tattaaatta aagctttgcg taaaacaact gccacgcctt
     9721 ctggagccca agcagctact actactggat tttctgcttc tgatttcaca cgtatccaac
     9781 caagacccga ataaacaata tccattttat ctttgattgt aaattcatga cgaactaatt
     9841 taggaaaatc tgaaacttcc ttacctgttg gtggtgtcaa taattttcca acatgtttag
     9901 tgtaaaattc atctgctcca actagtttag tgcggtgtaa attcaggtta ttatcaaaat
     9961 atgccgtaaa accttgtttt tgaccagaaa taaaatcaaa acgtgctagg ccagccaaga
    10021 ataatgtttg ttctgaatta agctgataag ttttgggttt tatttctttt ttaggactaa
    10081 catatttaag attttttgct gtcaaataat gagccatttg atgacgatga ataatacccg
    10141 gggtatcaaa aatatagcta ccatcatcaa gaggtatttc tattttatct aacgttgtac
    10201 ctgggaaacg tgaggtcgta ataacatcac gactaccagt aatttcacga ataatagcgt
    10261 taataagagt tgactttcca acattagtaa ccccaacaac ataaacatct cgaccatgac
    10321 gatacttttc aatcgtatca atcaaatcct taatagcatg atgattttgg gcacttgtca
    10381 agatgacatc aacaggacga agcccctctt catgagctct ctcagttaac cattgtgtta
    10441 cttttccatc tttaaccgat ttgggaagaa tatctttttt atttcctact aaaagaacat
    10501 cgtttcctgc aacgaaacga ctaagaccag gaataattga accattgaaa tcaaaaatat
    10561 caatgacatt caccacaagg gcatcactat ctccaacctc atgtagtaat ttcaaaaatt
    10621 catcatcagt gatatgaacg tcagtaatct cattatagtg gcgcaatctg aaacaacgct
    10681 gacagtataa ttctcctgtc tctaaacctt tttcaagtgc tgcacgaggt gtatagccag
    10741 cagcatcttt attttcagtt tgaatcctgg ctccgcagcc aatacaaaat aactcttcca
    10801 ttaaattcct ttttgatata cgattttacc gtaattctct tctatttttt tccaaacgcg
    10861 acgttcacgt aagcgattga acttggtatt ccaagcatca gatttaacaa ttggttttac
    10921 caatacggac tttattcctg cgcgataact agcacgaata tctgtcatca attgatctcc
    10981 aaccataatc acctcgtcac gatcaaagcc ataacgctca attgccatgt ttataccacg
    11041 agtaaatggt ttcatcgcac gacttacaaa atccacacca aaacgtgata ctgcacgttc
    11101 tacgcgagca tgattgttat tagacactac tacaactgaa atatccgctg ttgtcatctc
    11161 atctaaccat gcacgcacct cagcagtacc atctggatta ttccaagcaa tgagtgtatt
    11221 atctaaatca actaaaaccg cacgaatgcc atgacgtaat aaatcttctg ctcttaaatc
    11281 ataaactgcc tcaaccacga aggtcggcct ataatcatct atactcaatt tcatctccaa
    11341 aaaatatatt ttactattca ttctaccata attcttaaga gatagcgagt aaagaaaaaa
    11401 ggtctttaaa gacctttaaa ttagggtttc aaccccaaaa gatactatct tactcgtttc
    11461 attttcttca atgcaattaa atctttgtaa gagattagag ttacagcaag aaaaataaac
    11521 agcatcccaa gtaaatctat tggataaaaa atttctccta aaacaagtac tgtcaaaaat
    11581 accgaagaaa ctggctctac cgatgctaat aagctaccct tgacagcacc tacaatacta
    11641 acccctttaa ggaaaaaagt gtaagcaaaa atagtcccaa taccaataat gccaatataa
    11701 gcaagtatac tcatcacatt aatttgtaag gggtattgcc acgcttttgt caccaaacta
    11761 aataaaatcc cacctataaa cattcctgaa ccaataacaa tcgtacttcc ccattcatga
    11821 ataagtttca caggtaataa aatataaata gaataagtaa tggcggaacc aagcccccac
    11881 attaatcctt tcggtgtgat agctaaactt cctaacttcc catgcgttgc aatcatatag
    11941 gttcctaaaa ttgccatagc aattgctatt atttcaaaag atgttggtct ttgtctgttt
    12001 aggatacaaa caatactcaa aataagtatt ggtgccatat attgcaaaac cgttgcagtt
    12061 ccagcatttg ttaaatgaat cgctctgaga aaagcatatt gatttaaaac taaaccaaat
    12121 atagaaaaaa gtaaaacctg ctttaaaaat tttggctgtt tccacgctgc tactaaatgc
    12181 tccttttgtt ttgatctagc taaagacaag agaaagatac ctgttatcaa caatcgtaaa
    12241 gaagtcaaaa ggttaacgtg tactccgtgc gacatcaagt attgaccact aattccagaa
    12301 atgccccatg ctagtccggc agctaaggtc atcattgttc ctttttcttt tttagtcatt
    12361 tttcccctca tttttatttc ttaaacactg tagttaaagt catctgataa atatcttagc
    12421 ttcttaatta tttatcccta ggtgttcgtg ctaaattagc gaataaaaac cagccaaaag
    12481 gctgatttct attcttcttt taattttgct aattcttgcg ctaacagctt cagagcaact
    12541 tgtggattag cttgtccttt tgttgctttc attagataac ctgtgaaggc cttatcagca
    12601 ttgcgttttc ctgacttaaa gtcaatcaca gcagcttcat tatctgcaaa gacttgatga
    12661 ataattggga ttaatacttc aggatctgaa atttgaacta aaccagcctt tttaacgaat
    12721 tcttcagctg aaccaccatt tttagctaaa tgcacaaaaa cttttttagc aattttggat
    12781 gaaattgtac cgtcagcaat caagccaatc atctcaacca aattctcagg agtcagacca
    12841 atttcttcga ttgattttga ttcactgtta agaaattgcg ccacttctcc ttgtaaccaa
    12901 ttggaaactt gttttgcatc acccccaata gcaacagctt tttcaaagaa atcagaagtt
    12961 gcttttgtag ctgttaattg gctagcatca taagatgata gaccaaaaga cgatacatat
    13021 ttcgcacgac gttcctgtgg aaattctggt aattctaaac gaacttgatc aatccattca
    13081 tccgagatat caaatagtgg tagatctggt tcagggaaat agcgataatc tgatgagcct
    13141 tccttgacac gcatcaaaat agtctcacct gttgtttcat caaaacgacg agtctcttgc
    13201 tgaatttgac caccagatcg taaaacttgt gcttgacgtt tttcttcatg tatcaaacct
    13261 ttacgtacat tattgaatga gttaaggttc ttaagttctg ctttagtacc aaactcttct
    13321 tgaccataag gacgaagtga aatattggca tctacacgca tagaaccttc ttccatttta
    13381 acgtcagaaa ttccagtata ttggataatc tctttgagag ctgtcaaata agcataagct
    13441 tcctcaggag aacgcatatc agcttctgat acaatttcaa tcagtggaac accttgtcta
    13501 ttaaggtcaa cataggaata accatctgtc ccatgtgtat tttttcctgc atcttcttcg
    13561 aggtgggcac gctcaatacg aatttttttt cgtgtaccat cttctaattc gatttcaatc
    13621 caaccattgt aaccaatagg ctcatcaaac tgtgaaattt gataagcttt aggattatct
    13681 ggatagaaat aatttttacg atcaaaatgc atgttttgat ggatatccat attcaaggca
    13741 agtgccgcct tgattcctgc atcaataact cctttattca tgactggaag gacacctggg
    13801 aaagaccagt caattacatt tgtattagca ttttgttcct gaccaaaatg agcggaagaa
    13861 ggtgagaaaa ttttagagtt cgtatttaat tcaacatgga cttcaagtcc aatgactgtt
    13921 tcaaaattca ttagttctca cctccaaaaa ttttaggttg ttgtttatga taatctgtcg
    13981 ttgcttcgaa ggcagcagca acctgatata ttgtttcttc actgaatttc ggaccaatca
    14041 actgcatacc aaccggtaga ccttgatcaa aaccagcagg gattgaaata cctggtaaac
    14101 cagccaaatt aactggtatt gttagaatat cagctaggta cattgcaact ggatcgtgat
    14161 ttaaagaatc taagtcaaag gctgttgttg gagctgttgg acctaaaatc aagtcataat
    14221 ctgcgaaaac tttttcaaaa tcttgaataa tcaaactacg aacttgacct gcttttttat
    14281 aataagcatc gtaataccca gaagataaac tgaacgttcc cagcataatg cgacgcttaa
    14341 cttcgtctcc aaaaccttca gatcgtgtat tgacatagat atcatccaag tttttataat
    14401 tttctgtacg gtatccataa cgaataccat caaaacgttg taagtttgaa gaagcttccg
    14461 atgaagcaac aatataataa acagcaacac cgtatttaga atgcggtaaa ctaacttcct
    14521 caataactgc acctagtttt tccagatgtt ttgcggcctt aataatcgtt tctttaactc
    14581 cttgcgctat tccttctccc aaatattctt ttggtaaagc aattttcatt ccttgaatat
    14641 cttgaccaat tttagcagta aaatcgccta cagtacgctc agaagaagtt gaatctctta
    14701 catcatgccc agaaataact gtcaaaagct gtgcattctc ttttactgtt tgagacatcg
    14761 gtccaatctg gtcaagcgaa ctaccgaaag caaacaaacc aaagcgtgat acacgtccgt
    14821 aagttggctt cataccaacg attccattaa atgaagccgg ttggcgaatt gaaccacctg
    14881 tatcagagcc caatgataaa cgtacttgtc ctgaagctac tgctgctgca gaaccacctg
    14941 atgatcctcc cggaacttta ctatggtccc aagcgttatt tgtcttttta aaataagatg
    15001 tttctgtaga gcctcccatg gcaaattcat ccatgtttgc tttaccaatc acaatcatat
    15061 ctttagcata taacttttca acagccgtag catcaaagat tggctcatag ttatagagca
    15121 ttttagaagc agctgttgta agaatcccct ttgttgaaat attatcttta actgctaagg
    15181 gaatacctga cataacatta tcagcatcaa ttcctttatc atcaatttct ttggcttgag
    15241 ctattgccat ctcatcagaa atagtaataa aagatccaac cgcttgttca cgcgcatgaa
    15301 tatcttccaa tgtagctttt gttaattcag ttgctgaaat ttctttttta acgagaaaat
    15361 catgcaactg atcaattgat tgattattaa acgacattag gcatctcctc catcctcaag
    15421 aattgcaggt actttaatat aataatcttg atgctgtggt acatttttaa ataagtcatc
    15481 acgattatgg cctggttgag caatatcttc acgcatcaca gttttgcgat ctgccatggt
    15541 cgttgttact ggtacgcctt ctgtgtcaac ttcatttaag agttcaatca tatcaacaat
    15601 ctttgacaaa cttgacgcaa attctttagt ctcttgatca gaaaagcgca attttgacag
    15661 atgggcaaca tgacgcactt cttcttcaga aattttcatg agttctcctt atctctgtat
    15721 cattttcgtt ttaagatagt ctctactatt atagcatatt aaactctcaa tgttatcaca
    15781 ttatcccagc ttttcataat aaacgcttct tacctatgat aagaagcgtt tactgtttat
    15841 ttatctattg ttatatcatt tctgctttgt ttgattttag cctgtgcagc cgctaattta
    15901 gcaataggaa tcctaaacgg cgaacacgat acataatgta acccctgttg gtaacagaac
    15961 tcaatagatg atggttctcc accatgctct ccacagatac ctattttaag gtttggctta
    16021 acttcttttc ctaacctcac tgcctgctca ataaaccttc ctattccttt ttgatctaaa
    16081 acttggaaag ggtctttttt tagaagtcct ttatcaacgt attctcctaa gaattttcca
    16141 gcatcatctc gtgaaaatcc aaagctcatt tgtgttaaat cattggtccc aaaactaaag
    16201 aaatccgcaa actttgcaat atcatccgct atcatacaag ctcttggtgt ttcaatcatt
    16261 gtcccaatag ggaatgataa gttaattttt ttagatttac tctcctcaac aattgtctct
    16321 tcaatcagat cacgtattat agagatttct tcatgcgctg tcactaaagg aatcataatt
    16381 tctggctttg cctcatatcc ctctttcatt gcaagtatag cgccttgtac aagagctctc
    16441 acctgcattt ggtaaatttc cggataggta atcgcaagtc tacaaccacg atgacctaac
    16501 atagggttga attcttctaa tgtttgaatc cgtttttcaa tagctaaagt agatatcccc
    16561 atttgtcttg ccatagattc tatagactct ttatcatggg gtaagaattc atgtaaagga
    16621 gggtcaagca aacgaatcgt acaagcttta cctttcaata ctttaaaaat ttgataaaaa
    16681 tcgtctcttt ggaaagataa aagcctttct agagccttcc ttcggctcaa tatttcatcc
    16741 gctaaaatca tttctctaac gactggaata cgttcatcgt caaaaaacat atgttccgtg
    16801 cgacaaagac ctattccttc tgctccaaag tccaaggcct tttgagcatc tctcggatta
    16861 tctgcatttg aacacaccat catatctcga ttttcatcta cccatttcat aaatatctca
    16921 aaatcacgat ctacgctagt ctctgccatc ggaacttgtc ctatataaac atttcctgta
    16981 gcaccatcga ttgataacat ctcaccttct tttatcgttt gatgcccaat agaaatttct
    17041 cgtcttactt catcaaccaa taattgacta cagcctgcaa cgcatggttt ccccattcct
    17101 ctggctacta ctgccgcatg ggaagtcatt ccgcctcttg ctgttataat cccgtttgca
    17161 ctaaccatcc cctcgatatc ttcaggtgat gtctcttgtc tgaccaatag cactggattt
    17221 ccctttttca tctctttaac aacatcttct gcatggaaat aaactttccc ataagcagct
    17281 ccaggagaag ctggaagtcc tgtcgtcaaa ataatagctt tctttaaact tttcaagtca
    17341 aaagaagggt gtaataactg atctaattgt ttgggctcga tacgtagtat agcttcttct
    17401 ttactaatta ggccttcatt tacttgatct actgctattt tgatagctgc cttcgctgta
    17461 cgcttaccgc ttctcgtctg aagcatataa agattaccat tttcaattgt aaactccata
    17521 tcttgcatat cgcgataatg agcttctaac ttttgggtaa cgcttacaaa ctcttggtaa
    17581 ataataggca tttgttctgc caattttgat atggattgtg gtgttctgat acctgcaaca
    17641 acatcttctc cttgtgcatt aatcaaatac tcaccaaata aatttgctgc tccagttgat
    17701 gggtttcgtg taaaagcaac tcccgttccg ctgttatttc ccatattccc aaaaaccata
    17761 gcttggatat taacggctgt cccaagcgtc tccggaatat catttaattt acgataaatc
    17821 ttagctcttg gattattcca tgatttaaaa actgcctcaa ttgctaacaa aagctgcctt
    17881 ttaggatctt gcggaaattt ttctcctgct tctttttgat atagttcctt gtaaaattca
    17941 acaagtctct ttaaatctga accagttagt tctgtgtcat cttgataaca tttatctgtc
    18001 ttaagtcgat ctaaaatggt atcaaattta tattttggaa tgcctgtaac cacatctgca
    18061 aacatttgga taaaacgacg ataactgtca taagcaaaac gctcgtcttc tgtctttttc
    18121 actaaacctt ggacactacg atcatttaac cctaaattta aaactgtgtc catcatacca
    18181 ggcattgaaa aaactgatcc tgaccgaact gacaccaaca aaggatcatc aacacttcct
    18241 aattgcttgt tctgttcaac ctccaattgt gccattgctt gatcaatttg actaagaata
    18301 ctctctcgga tatggcaagc gttatcataa taatcattac aagcctgtgt tgtaatagta
    18361 aaaccttgtg gtaccggtaa cccaatactc gtcatttcag ctagattagc ccccttacca
    18421 cctagcagtt ccttcatttc cttacaacct tcatcaaaat gatacacaaa cttagtttcc
    18481 atcagcgtct ccttttacag atttttagct acttgtccat ttgtttttaa gatttctaaa
    18541 ataatcgttg ctgtttcttc gattgcttta tcagatacat taatgattgg gcaacctaaa
    18601 tttttcataa cttcttcagc atacctcgtc tcctccaaaa tccgttctaa actggcataa
    18661 tttgccgtac tagaaagacc taaggacttt aatcgaactt ttcggacttg attaagatga
    18721 tcaaccgaat tagttagtcc gataatcctt ctagaatcaa tcatacgtaa ttctttagga
    18781 acaggaacct ctggaaccaa aggaatatta ataactttaa tatttttatc tgctaaaaac
    18841 atacttaatg gtgttttaga agttcttgaa attcctataa taactaaatc tgcttgtaaa
    18901 atacctcgcg gatctctccc atcatcatat tttactgcaa attctattga ttctactctt
    18961 ttgaaataat cattgtctaa gcgtcgtaaa atccctggtt ctcccaaagg atctataccc
    19021 gatatccttg atattccttg aataacattt gttaataagt caacataagc ataatgttca
    19081 ctttcacatc gtttctgtgc ataggaagcc aaagcaacat caactaaact aaacatcata
    19141 aatactgtct tctggctagc ctcttcaaaa acttgttcta agcgttcctg actgttaata
    19201 taggaaaaac gttgaaaatg ccaatcatca tgtcctggaa attgtgacaa acatgctttc
    19261 gcaatagctt tggctgtttc ccctaaagaa tctgaaataa taaatatagt gagttgatct
    19321 tccatcagtt tcctcctaat aatctcttat ttctaaaaac aaactagcga ggattgtttt
    19381 agataatttc ccgatcactt taaatttttc gggatattgc ttatcatgac gaacgacagg
    19441 gagactatcc acttttctgc tcactaattt atcagctgcc gcaaaaagac tttcattttc
    19501 taaaacagtt gtcacgtgtg gcatacgtgt catcaccatt cctattggca ttttagaaag
    19561 atctcctccg ccaatactgg tttttagtaa atctttacgt gacacgactc cacagagaaa
    19621 atcatcatca tccaagataa aagcacaacc agcatcttcc ataaaaatat gtacaataac
    19681 atcataaact gaatcttttt gatgaactgt cagagggatc cccataattt ctgaaactgt
    19741 catcttttca aaatgacttg tccctattga agcatgatac tgtcctaaat aaaaataacc
    19801 aaccttaggt tttgcatcta tgatacctag catacttaaa accgccaaat cagaccttag
    19861 tgctgctctt gttactccta atttctcagc tattagctca ccagtaatgg gctgaaactg
    19921 tttgataata tcaataattc tttcttgcct ttgatttaat tgaataagat caccttcttt
    19981 atatattgta tactatttat tttatatgtt atattatata tcttttttta ctgaaatcaa
    20041 gtaaaaaaag atactatttt aaaaataatc atactatata tttgctttta gacatatctt
    20101 ttgttaaact ttcttcgaaa acgttttcga atataagtat cggaggttgt aatatgacaa
    20161 ttaaaaatct cactgttgca ggaagtggcg ttctaggtag tcaaatcgcc tttcaagcag
    20221 cttacaaagg aatgtctgtc accatatatg acattaacga tgaagcttta aataagggaa
    20281 aagaacgcat caaaaaatta gctaaagtct atcaatcaga aattgaaaca gcaaaagaag
    20341 catatagtga taaagctaaa agtatcaagt ataataaaaa cttattgcct agcttagacc
    20401 atatttttct ttcaaaagtt gccgattcat tagacttgat tgctgattta ccaaaccaaa
    20461 ttactttttc aaaaaactta gaccaagctg taagtgacgc cgatttagta atcgaagctg
    20521 tcccagaaac agttagtata aaagaagatt tttacaaaca attagctaaa gtggcacctt
    20581 caaagacaat ctttgcaaca aattcttcaa cacttgttcc aagtcagttt gctgatataa
    20641 caggacggcc tgacaaattt ttagcaatgc atttcgctaa caatatttgg caaaataata
    20701 ttgttgaaat tatgggacat aagggaactg acgacgaggt tgtcaaagaa gctttggcat
    20761 tctcaaaaga tattggtatg gtacctttat atattcataa agaacaacct ggctatattc
    20821 ttaattcaat actagttcca ttcttagaga gtgcattagc attgtactat aataaggttt
    20881 ctgattctga aactattgat aaaacctgga agttaggaac aggagcaccg atgggacctt
    20941 tagaaatttt agatattatt ggcatcgata ccgcttataa tatcatgaag aattattcag
    21001 ataccaccag cgatcctaat agtttacatg ctcaccttgc aaaaatgctt aaagaagaat
    21061 tcattgataa aggacgtacc ggtaaagctg ctggacacgg cttttatgat tacgactaaa
    21121 ctataaaaga ggttagataa aaatctaacc tcttttataa ttcaactaaa gaatagttgc
    21181 accaagaaca gttttaaaat gatttaaagc ccattgatga ttactatcat ttaagctggc
    21241 tacagcagct gctgggacct caattttata acctaagtta taagcatcaa ttgctgtgtg
    21301 taatacacaa atatctgtca gtactcctgt caatatcaaa gtatctactc gtctttcacg
    21361 taggcgaata tctaaatcag taccagaaaa ggcagaataa tggcgcttat ctatccaaaa
    21421 aacacgacta tcttccttaa tagtttcata tagagttccc aaaggaccat ataggtttct
    21481 accagaagtc cctttaatat tatgctcagg aaaaagattg gtctctggat gaaaaacatc
    21541 tccaatatca tgattatcaa tcgcaaaaaa aatataatcc cctgaccggt atgctttctc
    21601 tgtaacgtca gctatagctg aagcgataga ttgcgctggt ttacctgccg ttaatttccc
    21661 atcatctgcc acaaaatcat atgtgtagtc tattgagatg agtgcttttg tcatttttct
    21721 tcttatctcc tcttgatttc aaaaaaggaa ctgaaagcta ctcccagtcc catgctatga
    21781 ttaattatat tcttttaatt tgtcaaaaat accttcgtta ataactttga ggtatgttcc
    21841 cttcataccg agtgagcggc tctcaatgat tcccgcagac tccaatttac gaagagcatt
    21901 tacaataacg gaacgtgtaa ttccaatacg gtctgcaata acagatgccg ttaggcgtcc
    21961 ttcaagtcca tctaattctc ctaagatagc agctacagct ttcatttctg aataagataa
    22021 agtgttgata gccattgtca ccgctgtttg tttacgaata ttttcttcta gattttctgt
    22081 ttgaaggtta agtaattgaa taccaacaac ggtgcttgca atttcaacta aaatcaaatc
    22141 atcgtcgcta aattctttat cattacgcca gataataaat gttcccaagc gcattccacc
    22201 accataaatt ggagcgattg ttgtaatacc atcttggaag ttttcctttg tctcaactgg
    22261 gaaaattgac aaatcattat ctactgataa gttagcttct gtatcataaa cacgactagc
    22321 agatttaaca tagtaatctg ggaattgttt tgtttcaaaa aattcttcca cacggtcagt
    22381 gtttgtttta tatttcatgg catagccaag taagttacca ccaccattaa taatacatgc
    22441 gttacagtca ataatatcag ctaattgagc cgccattgta ttatatggta attcggcatc
    22501 taagctatct acggaacgct gtaagatgga tgtaattttt ctagtttttt ctaataaatt
    22561 cggcatttcc ttcatccttt gtatgttttg cgttacaatt atatcaaagc attttagctt
    22621 ttgcaaatcg atatcagtac attttaagaa cttctattgt taatttaatg tttatctaat
    22681 aaaaattcat tctagcgctt gtattggctt aaaaagcgag ccattttttc ttgtagttca
    22741 gtcaattcat caacacgtgg taagtaaaca attctaaaat ggtcagcggt attcatatta
    22801 aatcctctac cgtgtgttag taatactttt tcttgtttta agaaattaag cacaaactct
    22861 tcatcattat caatacggta catatctgta tcaattttag ggaaaagata cagtccagca
    22921 tttggtttta cagcagataa accaggtatt tcattaattg ctttatggat ataattacgt
    22981 tgctcgtaga tacgacctcc tggcaacaac atactatcaa tagattgctg tccgcctaac
    23041 gatgtttgaa taacctgttg agcaagaaca tttgaacaca gcctcatgtt agctaacata
    23101 ttcaaacctt caatataacc tttaacatgt tgtcgcggac cagacaatac catccaacca
    23161 acgcggaaac cacagatgcg atgtgattta gataagccag ataaagtaac tgtaaaaata
    23221 tcctctgcaa tagaagcaat tgggatatgc tccattccat ccataactaa acggtcataa
    23281 acctcgtctg aaaaaataat caagtcattt tgtctagcga tatccacaat ctcttgcaga
    23341 atttcacgcg gatagactgc tccagttgga ttatttggat taatgaggac gattgctttt
    23401 gttttggatg tgatttttga tttgatatca tcaatatcag gataccaatt agcttcctca
    23461 tcacaaatat aatgcacagc atttcctcca gcaaggctaa cacaagctgt ccataacggg
    23521 taatctggca ttggaactag aacctcgtca tcattatcaa gcaaagcttg cattgacata
    23581 gagattccct cactaacacc attaacaatg tatatatcat ccatgtcaac atggatgttt
    23641 tgtaactggt aatactgcat aacagcctta cgagctgaaa aaataccttt cgaatcagag
    23701 taaccttctg attcacgcgc attcgtaatt aaatcacgaa tgacttcatc tggcgcttcg
    23761 aagccaaagg cagctggatt tccagtattt aaccgcaata tcttttcacc atttgccctc
    23821 atgcggtccg cctcttctaa aacagggcct ctaatatcat aagccacatg ttccagcttc
    23881 attgatttat caaatatctt cataaaaata cctcgtatct tccattataa aacaaatatt
    23941 ctcaaaattg aaacatttac tgcggtttat caagcgatta agttaatatt aagctatttc
    24001 agaaaactaa cataaaacgc tttcattttt ctagaaaaaa cgttataata tagctataaa
    24061 atgaataacc aacatatcta acttactcat cactcactcc ttctgataag atggctagtt
    24121 gagtgcaaga ggttatctct tttggttata tttagaggag gacaggctta tgtcacaaaa
    24181 atatgaacgc atccttgtcg ctattgatgg atcaacagaa tctgaacttg cttttgaaaa
    24241 ggcagttaac gttgctcttc gcaatgattc tgaattaatc ttgacacatg ttatagatac
    24301 acgtgctcta caaagctttg cgacatttga tacttatatc tatgaaaaat tagaaaaaga
    24361 agcaaaagat gtccttgagg aatatgaaaa acaagctcgt gaaaaaggag cagataaagt
    24421 tagacaagtc attgaatttg gtaatcccaa aacattactt gcacatgata ttccagaaaa
    24481 agaaaaagta gacttgatta tggttggtgc aacaggtctt aatacctttg aacgtttcct
    24541 tatcggatct tcctcagaat acatccttcg tcacgctaaa gtagatttac tcattgtacg
    24601 tgaccctaat aaaacaatgt aaatttaata cgctcacttt ctaaaatatc ctgagttata
    24661 ttaactcagg atatttttat tttcgattta aggatttctg aatttggcta tctaattcac
    24721 gtcgaattga tggctcagga gcgaaatgtt cttcccagtc gtctggtttt aaaattttat
    24781 gagtaacagg atcaaaatga gctttgccat ctggaaagat tttacccata ttggcctcat
    24841 gtactgtatc aaaaagaggt tttgggtcga ctcccataag aacaaaggag ccataagtca
    24901 gataaagtaa atctgttaag gcatccactt ctcccactaa tggtgtttct ggatggtctt
    24961 tagaaataac cttatctctt gctttatcaa tagctaagtg taaattacgg atagattggt
    25021 caaatacttc ttggttacct tgactagcag catacaagaa ctcaacgatt tcttctactt
    25081 taaagtccga cctaaaacca gcttccttac tgtcatataa gcgtggcgtt tcaatagttt
    25141 caccatccat taatagatgg aaagatttta ctttattgaa attctcgtca cgactactga
    25201 atgttttttc aatctcaaat tggataagtc cataatgggc taatgcctta gagataccat
    25261 catcattatt gtcagcagtt gtataacgtg cattttcttt cacttgctga ctagcgttcc
    25321 ccatggcaac accaatacca acttgagata acatctcaat atcattatca gaatctccaa
    25381 atgctatcac ttctgataaa tcaaaaccat agcattgacc aacacgttca attcccttaa
    25441 cttttgactg cccttcagaa atgacatcca ttgagtaagg actgcttctt gttaatttaa
    25501 cacgaggaaa ttgtttttga attcgctctg attcgccctc tgttgcaact aaaacgacct
    25561 gataaatagg ttctcgtaag ataaccatga ggctatcaat attttgaggt ttaatccgac
    25621 gaataaaatg cttaaagctt cgttcaatcg cttttgccca ttttctcgga acaaggctag
    25681 atacaatctg ccccaaacgg ctagttccta gaccaataat acctgaaccg agtaatcctg
    25741 aggctgttcc taatgaaatt tctcttcttc ttgctccagc ataacgaatc agacgataga
    25801 cagttgtctt agataattga ttggtataaa taatttcact tctactatag atatattgac
    25861 cattataggt tacagcaaaa tctaaaccaa ggttttctaa aaaaggttgt acaaaactag
    25921 gacctcgccc cgttgctaaa ccgactagta ttccttggtc tttcaaattt cgaattgctt
    25981 taattgttga cttttgtaca ttcttacgat cattaagaag tgtaccatca atatcaaaaa
    26041 atactgcctt aattgccact gggtcaacat cccctctttt ttatgataaa ataaattata
    26101 tcacaacttg aaagaagtat aaaatcaatg aaaaaaatcc ttgttctaca cacaggtgga
    26161 acgatttcca tgaatgctaa cgagaaggga caggtcatgt ccagcgccga taacccaatg
    26221 aaatacgttg atttatcatt agatgacctt gatttgactg ttgtagattt tctaaatctt
    26281 cccagtcctc aaatcacccc tcatcatatg cttgatatct atcactatct gaagcaacat
    26341 gccagtaatt ttgatggagt tgtcattacg catgggactg acactttaga agaaacagca
    26401 tattttttag acacaatgat actccctaaa atcccaatca tcattaccgg tgctatgaga
    26461 agcacaaatg aacttggtag cgatggcgtt tataattacc tatcagccct ccgtgtcgct
    26521 aatagtacta aagctgccga caagggggtg ctagttgtca tgaatgatga gatacatgct
    26581 gctaaatatg tcacaaaaac acatacaaca aacgtttcga cattccaaac ccctacacat
    26641 ggcccccttg ggattattat gaaacaagac ctgcttttct ttaaagcaac tgaagagaga
    26701 gttcgttttg atttagataa aattacagga actgttccta tcgtgaaagc atatgctggc
    26761 atgggtgata gtggtatcat tagcttttta aattctcaaa atattagcgg attagttatt
    26821 gaagcactcg gtgcagggaa tatgccccca aaagctgcac aagaaatcga agaattaatt
    26881 gagcaaggtg tccctgttgt tctagtgtct cgctgtttta atggtattgc tgagccagtt
    26941 tacggatatg aaggtggcgg agcaaaatta caagaatctg gtgtcatgtt tgttaaagaa
    27001 ctaaacgctc ccaaagccag actcaaatta ctcattgcac tcaatgcagg tctcacaggg
    27061 caaaatctaa aagactatat tgaaggttga taaaaaactc tagaaatatt tctagagttt
    27121 tttattaatt ttcagtttgt aatactctct caacactgct aataggcatt tcttgattcg
    27181 ttattaattg aaatgcttct gctccttgat aaatcagcat ccctagacca ttataagcat
    27241 gtttcactcc attttgtcta acaaatcgta acaatgctgt ttctttaggc ttgtaaacca
    27301 agtcgacaac tactaaattc ggtgttaaaa tttctggatc gttaattaaa ctataattat
    27361 ctaatggcct cattcctaaa ctagtagcat caatataaaa atgactagtt ctaatagcgt
    27421 ctttaaatgc tgtcttattt tctagataat caacgactac ctttatttga aactgttttt
    27481 taattttatc tgataagtca atgaccttat cgtaatttga gctgttacga ttaaataatc
    27541 taatttccgc aactccctcc atagctgctt gaactgcaac tgctttacct gaaccaccaa
    27601 taccagctat tgtaattatt ttatttttag cactgaaacc ttgagctgct aaagctttaa
    27661 aacaaccaat gccatctgtc atatggccta ctaaacgtcc ggttccacct tgattaacga
    27721 tagtatttac agcacccact aatttagctt gaggagataa atcatctagc aaagggataa
    27781 cactctgttt aaatggcatt gaaacattaa caccacgaat actcaatgcc ctgacacctc
    27841 gaacagcttc tgttaattta ccctcttcta cttcaaatgt cagataggca taattcatgt
    27901 ttttttcttg aaaagaggta ttccacatta acggggatag agagtggcgt gcaggattgg
    27961 caattagacc agttagtaaa gtttccccat ttaatgaatt cattgtataa ccttctttct
    28021 taacaattta tttcagttca ttttaccaaa aactgactca attaaaaaga gagtatgtaa
    28081 tttatactct ctcccttagt ttaaggcaat atattacctg cactacggta aatatcatac
    28141 cattctttac gggttaaagc gatatcatta gccttatcaa tcgcctttaa acgagatgga
    28201 ttcattgaac caacaatcgc ctgcattttg gcaggatgac gtaaaatcca tgcaatcaca
    28261 atagcttcgc tgctaacatt gtagtttttt gccagtttag ctatggtttc atttaattcc
    28321 ttataatccg gatgattaac aaataaacca cgagataaat caatttgaaa tggagaccaa
    28381 gcttgaatgg taactctttt aagacgacaa taatcaacaa tgccatcatc atgttcaatt
    28441 gaagctttat ttaacatgtt aacatttaac cctgcatcaa acattggtgt atgtgctggc
    28501 gataactgaa gttgattcac tgctaagggc tcgtcaaggt aggattgtaa caactccatt
    28561 tgaaaccggt tttgattgct aacaccaaag tgttttactt taccttctgc cctcagtttg
    28621 gtaaatgctt ctgctacttc ttcaggctca actaatacat ctggacgatg aagaatgaga
    28681 aaatctaaat attctgtccc taatctttct aaaataccat ctactgatga taaaatatac
    28741 tctttagaaa aatcaaagta accttctcgt atcccacatt tagattgaat aatcattttg
    28801 tcacgattga catttaaatg tttgattgca tctcgaaagc gtaattcaga tagccctccc
    28861 ccataaatat cagcatgatc aaaaaaatta atacccaaat ctaatgctgt tccaactact
    28921 tcttcagctt gttttccttt caagtcactc attctcatac aacctaaagc aattcgagtt
    28981 gcttgaatgc ctgtttgtcc aatgttttgt aacatatgca ccccattcta atctgtttgt
    29041 attaacagaa agattaatca attcattata acaaaatttc tatgaaaagg cttactatta
    29101 caaatgcaaa aagctgagta aatttaactc aacttttttt ctgatactca ttattgacta
    29161 aataagtcgg taagaaaaat ctattctgaa aagtactatt ataaattaca gtaatcatta
    29221 ttgtcataac atacctccac atctgtgata ggaatatcat atgacagcaa gcttaaactg
    29281 agcttagcac agccttaata gaaacttaat caaaatctga atgttggcgt aaattatcta
    29341 gaataagtgc ccattctgta ttttctttcc aattattatc tttgacaata tcactagcaa
    29401 cacgtctggc ttcttctaaa ataggataat cctcaacgat atcagctact tgaaattctg
    29461 gaattccaga ctgacgagta ccaaagattt caccagaacc acgcattttt aaatccgact
    29521 cagctaaaac gaaaccatct gtcgtttctg tcatgattgt cattcgtttt ttcccagaat
    29581 cagttttggg attagccact aaaacagcgt atgattgttt atatccacga ccaacacgcc
    29641 cacgaagttg atgtagctgg cttaatccaa aacgatcggc atccataata atcatgatcg
    29701 ttgcatttgg gacatttacc cctacttcaa taactgttgt agatactaaa atatgacttt
    29761 ttttatcctt gaaatcttgc attatagcat ctttttcatc atttttcata cgtccatgta
    29821 caagagcaac cttagcaatt ccttcaaaat aagtagataa ttcagcatgc aatgctactg
    29881 cattctttaa atctaaagct tctgactctt caatcaacgg tgaaatgaca tacacttgag
    29941 catctttttg caattcacct tttacccatt ccaacacagt acctagctgc tcgtgtttca
    30001 cccagcgtgt aataataggt ttacgaccgg caggtaattc atcaataata gaaacatcca
    30061 tttctccaaa agctgtgatt gctagagttc ggggaattgg ggtggctgtc atcattaaaa
    30121 catcaggatt ttctcccttt tcacggaaaa ttctacgctg tttaacacca aaacgatgtt
    30181 gctcgtctgt aattacaagc cccagtttat ggtactgtac cgagtcctgg ataagagcat
    30241 gagttcctac aatcatatca accgagccat ttgcaatagc tgctaaaacc gtacgcttaa
    30301 cagctgcttt catacccgaa gttaatatag cgattgataa atctggaaat aactcttgca
    30361 gactaatgta gtgttgttca gctaggattt ccgttggaac catcaaagcc gattgaaaac
    30421 ctgctgtata agctgcatac attgatagac cagcaatgac tgtctttcct gatcctacat
    30481 ctccttgcaa taaacgattc atatgagctc ccgatgacat atcagataat atttcatcta
    30541 aagagcgctt ttgagcattc gttagaataa aaggtaaaga tgaaatcttt gtctccatag
    30601 catgtttact ataaagaata gacaaaccat ttgtttcaga tttattttcg gctttcaaaa
    30661 cttgaaggtt catttgaaag taaaataatt cttcaaattt aatccgacgg agcgcttgct
    30721 tatactctgt gatatctttt gggaaatgca tagctaaaca agcctgacta cgacccatca
    30781 atcggtattt tcccaataaa gtagctggta aattttcctt caattccaaa tgcgcattga
    30841 tttcaaaagc tgacttaata gctttaatca aagtagactg tgaaattccc tgagatacgc
    30901 gataaacagg ttgcatgtca tcttcaactt gagctaaaac cttcatccca gtaatagctg
    30961 atttagttgc atcccattta ccaaagacag ctacctcttg accaagttct attttatcag
    31021 ctaaataggg ttgattaaaa aaactaacat ttaagacagc ttccccttga cgcaatttga
    31081 aacttaaacg gttacgttta aaaccataat attgtacatt agctggagta acgactaagc
    31141 ctgtaatgac tgctttttca ccatccacta aatcaaaaac agattttgat ttaaaatctt
    31201 catagcgaaa cggataataa agaag
//
LOCUS       DATRSG010000015        30935 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRSG010000015 DATRSG010000000
VERSION     DATRSG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30935
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.015"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(37..603)
                     /locus_tag="VBD89_000696"
     CDS             complement(37..603)
                     /locus_tag="VBD89_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7135246.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(596..1531)
                     /locus_tag="VBD89_000697"
     CDS             complement(596..1531)
                     /locus_tag="VBD89_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO7135247.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(1624..2265)
                     /locus_tag="VBD89_000698"
     CDS             complement(1624..2265)
                     /locus_tag="VBD89_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7135248.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(2246..2830)
                     /locus_tag="VBD89_000699"
     CDS             complement(2246..2830)
                     /locus_tag="VBD89_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7135249.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(2900..3045)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3114..3713)
                     /locus_tag="VBD89_000700"
     CDS             complement(3114..3713)
                     /locus_tag="VBD89_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEO7135250.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKIGNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(3706..4377)
                     /locus_tag="VBD89_000701"
     CDS             complement(3706..4377)
                     /locus_tag="VBD89_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135251.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(4367..5242)
                     /locus_tag="VBD89_000702"
     CDS             complement(4367..5242)
                     /locus_tag="VBD89_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO7135252.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(5280..6788)
                     /locus_tag="VBD89_000703"
     CDS             complement(5280..6788)
                     /locus_tag="VBD89_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO7135253.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(6829..11133)
                     /locus_tag="VBD89_000704"
     CDS             complement(6829..11133)
                     /locus_tag="VBD89_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEO7135254.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGAVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDISVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(11153..11722)
                     /gene="lepB"
                     /locus_tag="VBD89_000705"
     CDS             complement(11153..11722)
                     /gene="lepB"
                     /locus_tag="VBD89_000705"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7135255.1"
                     /translation="MKRQISSDKLSQELDRVTYQKRFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKTGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(11706..11978)
                     /locus_tag="VBD89_000706"
     CDS             complement(11706..11978)
                     /locus_tag="VBD89_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135256.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
     gene            complement(12260..13408)
                     /locus_tag="VBD89_000707"
     CDS             complement(12260..13408)
                     /locus_tag="VBD89_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7135257.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
     gene            complement(13398..14246)
                     /locus_tag="VBD89_000708"
     CDS             complement(13398..14246)
                     /locus_tag="VBD89_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7135258.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            complement(14243..15667)
                     /locus_tag="VBD89_000709"
     CDS             complement(14243..15667)
                     /locus_tag="VBD89_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO7135259.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            complement(15667..17031)
                     /locus_tag="VBD89_000710"
     CDS             complement(15667..17031)
                     /locus_tag="VBD89_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO7135260.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            complement(17033..17980)
                     /locus_tag="VBD89_000711"
     CDS             complement(17033..17980)
                     /locus_tag="VBD89_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7135261.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            complement(17989..18924)
                     /locus_tag="VBD89_000712"
     CDS             complement(17989..18924)
                     /locus_tag="VBD89_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7135262.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            complement(18926..19984)
                     /locus_tag="VBD89_000713"
     CDS             complement(18926..19984)
                     /locus_tag="VBD89_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO7135263.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            complement(19984..20706)
                     /locus_tag="VBD89_000714"
     CDS             complement(19984..20706)
                     /locus_tag="VBD89_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO7135264.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            complement(20706..21530)
                     /locus_tag="VBD89_000715"
     CDS             complement(20706..21530)
                     /locus_tag="VBD89_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO7135265.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            complement(21555..23288)
                     /locus_tag="VBD89_000716"
     CDS             complement(21555..23288)
                     /locus_tag="VBD89_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO7135266.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGI
                     KNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVG
                     LTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRI
                     PIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLN
                     SSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKK
                     GVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEY
                     ILGRLNADKKVSLYQPNGFWINTQKTIKSLNKEYLNQNAYHIRGNNIIGIDGYSNDID
                     LNPRLWAYNYKKQVKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQSNKPFYFTVNKDS
                     VSKAQFGNYNINGYIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVALFIFY
                     ITAIRFVFKRM"
     gene            complement(23281..23634)
                     /locus_tag="VBD89_000717"
     CDS             complement(23281..23634)
                     /locus_tag="VBD89_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO7135267.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            complement(23631..24362)
                     /locus_tag="VBD89_000718"
     CDS             complement(23631..24362)
                     /locus_tag="VBD89_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7135268.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            complement(24364..25305)
                     /locus_tag="VBD89_000719"
     CDS             complement(24364..25305)
                     /locus_tag="VBD89_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7135269.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            complement(25295..26449)
                     /locus_tag="VBD89_000720"
     CDS             complement(25295..26449)
                     /locus_tag="VBD89_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO7135270.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            complement(26566..27420)
                     /gene="rfbD"
                     /locus_tag="VBD89_000721"
     CDS             complement(26566..27420)
                     /gene="rfbD"
                     /locus_tag="VBD89_000721"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO7135271.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            complement(27510..27851)
                     /locus_tag="VBD89_000722"
     CDS             complement(27510..27851)
                     /locus_tag="VBD89_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO7135272.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            complement(27960..29069)
                     /gene="rpoD"
                     /locus_tag="VBD89_000723"
     CDS             complement(27960..29069)
                     /gene="rpoD"
                     /locus_tag="VBD89_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO7135273.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            complement(29077..30885)
                     /gene="dnaG"
                     /locus_tag="VBD89_000724"
     CDS             complement(29077..30885)
                     /gene="dnaG"
                     /locus_tag="VBD89_000724"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO7135274.1"
                     /translation="MGYFCGGHDLAIDKEKISEIKNSVNIVDVIGEVVGLTKTGRNHL
                     GLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKFVEDYQHISFLDSVQVLAERSGI
                     PLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLMTTNDGERARQYLAERGVTEDLI
                     KHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNYSENSNQFYDSFNNRIMFPLTND
                     IGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKSYELYHLDKARAVINKAHEVYLM
                     EGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRFTKKVVLTYDGDRAGQNAIDKSL
                     ELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLENGRISNTEFYIHYLKPENTDNL
                     QSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPDFDYFQVEQSVNNERLHHRSQQQ
                     ASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNHPYLLNEFRNRDNFYFDTTEIQV
                     LYELLKESGEITSYDLSQESDKVNRTYYIILEEQLPVEVSIGEIEAVEKARDRLLKER
                     DLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME"
BASE COUNT         9800 a         5991 c         4688 g        10456 t
ORIGIN      
        1 tagcaattat catttttgct aattgcctaa agatagttac acctcatttt catatgattg
       61 taacttttct accataatta aaaagggggg cgtattaact tgattgagtg gttgataaag
      121 cattgtagcg aaattattct gtggtaattg ttccacgaat gctatcaccg catctctctc
      181 actttttcca ccatcatgac cataataaac cataagtgat agacgtccac ctacttctaa
      241 aatgtctaaa acgtttttga ttgccttgat agttgtgtgt ggttttgtaa taactgtttt
      301 atccgctgag ggtaagtatc ccagattaaa tatagccgct cttaaaggtg tatgaacata
      361 ctgttccaaa ttttcatgcc catccaagat aagttcagcg ttagagatcc cttgctccgt
      421 gagctttgct ttcgtttttg ctattgcttc ttcttgaata tcaaatgcat aaactttttt
      481 agaacttttc gctaaaaaaa cagtatcatt cccatttccc atagttgcat caagcgttat
      541 tgcttcttta tcaataactt ctgctaaaaa atcatgagaa agatgaattg gtcttttaat
      601 catgttttac ctcaacttta catccttgat atgactgtct tttctccatt tctttgtcaa
      661 tggcattaag cacttcccat ttattaaggc tccacatcgg tccaattaac atatgacgtg
      721 gcgcatcacc agtaattcgg tgaataacaa tatgttttgg aataatctcc agttggtcac
      781 aaataatcga aatatagtct tcttgactta acaaacgtaa acgaccttca tggtaatctc
      841 tctgcattct tgtattaatc ataagatgca gaaggtgtaa ttttataccc tgaatatcat
      901 tatccgtaac acatcgacga acattttcca ccatcatatc atgtgtctcc cctgggagac
      961 catttattag gtgagaaacg atttctactt taggagctaa ttctcgtact ctcttaacag
     1021 tttttttgta aaggtcatat gaatgtgctc tatttattaa tgcagaagtt gcttcataag
     1081 ttgtctgaag gcctaattct aaagtcacat gcattctttc agaaagttca gcgagatagt
     1141 atatagtttc atcaggtaag caatccggcc ttgttccgat gttgatcccg ataactcctg
     1201 gctcattaat agcctgttcg taacgctctt taattatctc taacttagca tgggtattgg
     1261 taaaattttg aaaatagact aagtatttat taacctcagg ccactttcta tgcatgaaat
     1321 caatttcttt atagaattgt tcacgaatag gagcttctgg agcaactata gcatcccctg
     1381 aaccagaaac tgtgcaaaaa gtgcaccctc ctctagcaac tgttccatct ctgttaggac
     1441 agtcaaaacc agcatctata ggtaatttaa atattttttc tccaaagagt tctcgataat
     1501 aatcattaat cgcacgataa cgttttttca taggataatt gtatcacaat tttaactaaa
     1561 ataacctcac tactacaata aaactaaaaa agattggaac gtcagttagt cccaatcttt
     1621 tatttacttc actttcttta accaatcctt ggataaaaag atatacgcag ttagattcaa
     1681 aataccataa gcaagtataa aaccagctaa aacatctgtc ggaaaatgaa cccctaggta
     1741 aatacgagat aacccaatta aaaaaatgag caaacccaat gtaccttggc acaacagttt
     1801 ccatatactc ttaggcatat agtactgcaa taaaataata ctactcccaa atatcataaa
     1861 tgttcccatc gagtgcccac tgggaaacga atagccacct gcaaatacta aatgggttaa
     1921 agttggtctc actctttgaa aaataagttt taaagaaagt atacatatac cagagataat
     1981 agcatttact gcgataaatc tagcttgagg ataccacttc ttaaggtaac agaaagtgac
     2041 gctcataatc gcaatagcta tctggcttac agtattacca accacagtga ttaacttaaa
     2101 aaatcttgta gaaagatttg gcaactgtcc tctaacactt tcttgaatag tttggtcaaa
     2161 tgcgattaca gtgtcgggcc aatatttgat gaccaatcct aaactgaaaa ataagataat
     2221 agcaataaat gcttgaataa gtttactatt ttgacgagat aacattagtc tttttatatc
     2281 tttctaatat tggcaaacaa gccacgtaag ttagatagaa aacaatcgaa attaaaattc
     2341 cctcaacgat attaaatgga ataaccattg ttaaaaggta attgcctaca ccaataaatg
     2401 ttctgatatc aaagttagca aatatagcat acaaaggaat cgcaaagaca tagttgagag
     2461 ctaccataga tacagtcaag ctaactgtac caaataaact agctttaata aaatcttttg
     2521 cactctctct atttttccag aaaatagcga aacttgctaa aaatagagct agagcaacca
     2581 tattcatcgg taaaccgata aaggtttctg gaccacgatt agcaagtata acttttaaaa
     2641 gtgatcttaa taagagtaca ccataacttg atttcaaatc aaataaaata aaagcaacta
     2701 acatcggaag gattgaaaaa tcaaccttta aaaattctgc tcctggtatt aatggaaatg
     2761 aaaccatcat caatacaaaa gataaggcag aaagaatggc gattgtcacc attttacgtg
     2821 tatttgtcat aaaaaaattc ctccaattta aataaattga aagaagctcc aaaggtaagc
     2881 gtaggtacgc gaaaaaaacc tttgtcttct cccatccaga ctttactgtc ggttgtggaa
     2941 tctcaccaca tcagctttcg ctcgcggact gatgcttcac aactgacaaa taagttggaa
     3001 gcgattaccg ccggtcggga attacaccct gccctgaaga cacctatagc ataacaaaaa
     3061 aaacttgcaa ttgcaagttt tttaatcact aattagtagt agattgtata atattaattt
     3121 ttaacatcaa ttaattgaca gcgcactaat actctagcta ctcctgcctt tgtacaagta
     3181 aacaagctta agtcccaatc attgtctgat gtggcagttt tataaacttt ttcaatcgct
     3241 gttggttcaa taatttctct attaccgatt ttgtagtgat agatttgccc tgttgtagtt
     3301 gtaaaataaa catccgttcc catatctaca ttttttaaag catcaaaatg ataaggaaaa
     3361 ttatgcgcac aaatcaccat gttattagtt aaataagaac cataatacct tgtaggcgtc
     3421 ttagacagtt gttcaaaact ataattagca gctaccggta aatgcagttt taagttcgga
     3481 atatccagag ttcccaagta atctgtttta tccaactttt ttacaggtaa ttctccattt
     3541 tctgaaccct ttacttgatg cgtaatagat ttatcaagca ccttgacaat atgctgagaa
     3601 gttaaatcag cttgatgcgc ctgattaata ttataccaac cccaatagat tccagaactt
     3661 accagaataa ttccgagtat agctaaaaaa tttgctgaat atcttctaat cacgtcttct
     3721 tctccatttt aaagctatta ttaaacacag aagtcctgac ataattagta taggtattgg
     3781 ccaccatact tgtccagtaa acggaagctt tccctttgtc tgatgtgtta ctgtaggagt
     3841 aattgtctct tcttttttag gtttagcatt taaagggctc attttctcaa atgctgtaat
     3901 atcgtacttc ccatccttag gtattgatag tataaaggga gacattagtt cataaccttg
     3961 agccgtttta gtctgaataa atagataaat cccttgagga agattgttcg caacaatacc
     4021 ttcagccggt aaattatcaa acgtttgtaa aggttgagtt ttatgaacag cttttgttag
     4081 tagattgacg tatttggctt ggttactatc aaggtttact tgtgttagat catcgtcttt
     4141 tattccaata ccttgaaatg gggtagttag agtaaaaact tgattaccat caacatcttt
     4201 agcttgtgct acttggtaaa caagtaaatt accgccagcg ataccttgat tattatactt
     4261 attttgtata gtaatagaac ccgttttcat ctgatcattg gtatcagcag acacaagttg
     4321 agtacttaga ctaaataata agagaagagt tatctttagg atctttttat aaatcattgt
     4381 tctcttcctt tctcattgct tgttttaaaa ttttcttacg ttgacgtgct ctcctagtta
     4441 cttctaaaga gattaaaagt aaaatcaaag taaggaaaat agcgataaat ggtgcgatat
     4501 aaataggctc tatttgtatt gcctctgcta ctaccaaagc gttaccatta tcgtttggta
     4561 cacgatgtcc tctcactagt aaccgatggg tattaacgcc atatggtgta cacgtcacca
     4621 aagtttggta gtctttacct ttaacaattt gtaaatccct caaatcatcc ggtttaactg
     4681 ttctgatttg atccacttga taagtatatg tttcatttaa gatactgact gtccagtggt
     4741 ctccaacttt taacttatcc aaatcagaaa aaagccttga agagggtaaa cctctatgtc
     4801 ctgataaaat agaatgagtt gagtctcctc caattggaag actacttcct tctaaatgac
     4861 caatagaagt ttgaagcact ttttcacttg taccatgata aagtggtaat tttatgttta
     4921 tctttggaat tgaaatataa cccatattac ccgttttatc gatagccagt tgtgaattat
     4981 aatccaaacg ctcttggcta gtcatgtgcc acttcattcc tgaagtttta aattgcttat
     5041 tatattcttt ggctcggtta ataatttttt tataatcgtt ttcatccata tgcgttacgc
     5101 ggtcttggta atccataatc gctcgagatt ggtgaaatga attccaataa ttagcaagtg
     5161 aaggataagc cattaagcct acccccactg caattatagt gacaagcaaa atggatacta
     5221 aatgttgtct tatttttttc atatatttta aatctgtacc actttgctag cccatcttat
     5281 taagaacgta aacgacgacg agcaacaagc acgatacctg ctcctattac taaaattgca
     5341 cctataatgt agaaaattgt tgtaccaata ccacctgttg aaggcaactc agtacctttg
     5401 ttattttcaa cagttgggtt aactaaaagg ttatctgaat tagtcgtatc agtggctcca
     5461 tctcctaaaa taaccttctg agagttatct aacaaattgt aacctaaggg agcctttttc
     5521 tcaactagat agtatgtacc ttctttcaag cctgtaatgg taattatacc atctgctcct
     5581 gttgtatatt ctgttgcatt agcttctgtg ccccattcaa cgttatttgt atcgttaaag
     5641 tttagaaatt gacccgtagc attctttaaa acaaatatag caccttgtaa tgaagctttt
     5701 gtggaaccat caattttttt tatagtaatt tgaccatccc tcactgttac tttttgacct
     5761 gggtcatcat tgctagtatt ggggttgatg gtcgcaatgt ttgtattttc tggtaaatca
     5821 gctgaacctg gtttagctcc actctttaat actcctgtat aagtgactgt gattgtattt
     5881 attcccttat aaaaaaagtc atcattagct ccattttgag tatttccggt tggagtattg
     5941 gtagctgccc acggaatagt aatcgtgaaa ttattatttt cctctaacag gttatacttc
     6001 ccagttgctt tttccgaacc ttgagttaga gttgtaatat tccctgatcc atcagtaata
     6061 gttacttcat aagacccttc gttcaaatca actacagaag cagatggcat agtatccttt
     6121 ataacatatt gatacacttt ttctgtacca tgataattga ctgcattctt ataagtaata
     6181 gtatatttga ctgtatcacc aaccgagtac gttttttgat ctacagtttt tccaccacca
     6241 tctccccatg tcgcatcagt attcttttca tgaatagtag catttggagt tacagatgta
     6301 accataatta cagctccatt attaacagtg ctagaaacat aataatatcc atattgggaa
     6361 acattaataa cctcagtacc atcattattt gactcagtaa cagtggaaac tggtgtagta
     6421 ttagctgata tagatttagc ccatgtcgca atctcatttg ctgacgcagt atctttttta
     6481 gttacatatg ttctccctcc attagtagtt gtcgtaaaaa gagaattaaa atcagttgaa
     6541 gctttatact cagcttcttt accttgagga attaaataag aagctccatc tttattcgaa
     6601 tcagatacat ttgcattatc tatttctgca tcaaaaactt tatatgcttt ataggttgcg
     6661 cctttttgag tatcttgaac tgtaattgtc cctgtctcag cggcaaaagc tatcggcgta
     6721 actggtgata cagccatacc aaatgctaaa ctcgccacta acagcgattg aatcattttc
     6781 tttttcattg aaatctttct cctaaaatca tattgatgaa tgattaattc atattttttt
     6841 tcgatagtat aatattaatc ctgatggtag agctaaagct aaaccaacta ggatataaat
     6901 gtgtgttcca atacctccag tactaggcaa ttctgttcct ttactgttag taattttaaa
     6961 agtatatact gtacttccat ccactaaatt ctcttttatt ggtgtcgcat tattaccatt
     7021 ttgttcaaag gtaactcccg tagaaatcac taatactgat atatcatttt taggtagtag
     7081 gtaccctgga ggggcctttg tctctgttag gtagtatttt cctactggca aactgaggta
     7141 gttattagca tccactaata acaagccttt atcgtttgtc accagccctg aatacatagg
     7201 atgtgaagct ttattcccat tagcatctga ttcataaata tcaaaaactg cacctgctaa
     7261 aaaattatta tcattttcga cattaacttt ctgtagtcgt actttttgct tgatacgtgt
     7321 attggtaaag ctaatatcta cgtctcctga aactgtcagg gattgtaagc cggtagcatc
     7381 ataagtttta tcagcttcac cagttgctag atttttttct gtaattgact cagatacttt
     7441 aaattcatcg taggcttgtt catctattga tatagaagtt ccataaggta ctttaaattc
     7501 cttagtctga ccatctctca gcggaaaatt ctcttgttgc aacgtttcac ttggattaaa
     7561 caagaagtct ttcgtcttat cttcatctag tccaacgaca gttttactta ctctgacggt
     7621 gtattcttta ggttgccaaa cagcatataa ggtatttgtt gcatcagggt tgttatcaat
     7681 acctattgat tgacctgctg taaattccac acgtcctgta tcagctaaat ccttatcatg
     7741 atgccaacca ataaggttgt aacctgtcct tgtaaagtat tggttttcag gaattgtagt
     7801 tgtgctattc aactccatac gcggtgtctc cacttgtgtt accacattac cattttctac
     7861 tctagtacca ccgttaccat tgtatttgat ggaggtatct tctaatttga tatctcctac
     7921 tggaataatg acaggtttta tggtgatatt tttattagca tctgctaaat gggcgtcaat
     7981 atcaatggaa tcatatgggt tataaatttt accattgtac caccagccac ggaaacgata
     8041 gccatctggc attgtcggac gtctcagtaa ggctgaatgg gagccatcgt cataggaatc
     8101 agggtcagta ggctcattac ttgtttgtaa ttgctgacca gaagcatcca atgctggctt
     8161 tccatctgta ccaacagcat cattgctgta tataatatga taatctccag cctttcgcca
     8221 aatagctctt aaattgatat cttgagttac agcacctgaa aagttataag gtctaatact
     8281 accatctgga ttaacataat accaaccgac taatttgtaa gcgtctttta cgtacttgta
     8341 cttagttgtt gtatcaacat ttgagagact agtatctgtc gtataatagg catctttagt
     8401 tgagtcggga tctttatctc gtgaatcata cttataataa tatgtacctg aaggatcttg
     8461 gatataatcc cttgtaatat ctgtataatc cggaatacga tcaccataat gcaaatctaa
     8521 ataggtatca tctgtttttg ataatcggcc tccgtttgga tcaatattga cacgatatgt
     8581 taccttttgc caacctgcat agactttaac atcatgagga ggcatagtcg tgttaaagtc
     8641 aaatacttgt gtttgtgctt ggtctttata ccatttacca tcccaaacat accctggtcg
     8701 actaggttta ggttgaacag ttgtcgtatc gggggcataa gaggacaaat tttgctcata
     8761 tagaacatcc tttactggaa aattaggaag ctctgtatta tcaagcggat ctaaatattt
     8821 aatcttgtat gaattacgtt cataccatac cactaagttc aaataatctt tgtggtaggc
     8881 accatatcta tcatagtatt catctgaaat aggaactttt actcctgcac tcgtttgact
     8941 tgcgtactga tcaaaaaggt aattatcagg atataaacgt tgataatatt taacattaaa
     9001 ccctagatat ttttctgtaa aggtaaattc atctgcccca gcacctccac cggtatctgc
     9061 taaagaataa gtgccatcca aaccttgttt gaagaaccga taagtttgaa ctatattccc
     9121 ttttggaaag agtttgataa catctgaatc aacggtatca ttaggtaaaa tgaactcacc
     9181 caaataactc atcccatagg ttccaacttg gttatttcca acattggcta aataacccca
     9241 tgcacccgtc ttccattgat aaccattagc ggccaaggtt gtaccgtaaa gtcctgtgta
     9301 agtatcagca tcagagctcc aattatacga atagtaatat tctggcaagg aatatccgcc
     9361 atattttgca aattttattg tgatgagttt tctatcataa taaacattaa caacacttga
     9421 accatcgtct tttatcatga cagaagtttc tgtcctcgta ttattaactt taaagccagt
     9481 cggtagttta tcattaatat cttgttgtgt tagcgtctga ttagataaag ataggcctga
     9541 tcgtgttact tgccctgcgt actcatatgt cttttgcgca tcagtagcat ttttattatc
     9601 cgttgctgat tgttgccagt agtttatcgt gtaggttgtt tgtgcgggcg accaatgtgc
     9661 atatagcgtc gtatccttgg tcaagacttg attaaaatca aaggctgccc caccactagc
     9721 agctgtgtac caccctgcaa aagtataacc tggcctcgtt ggatcattag gcttaattgt
     9781 cgaagcaggt tggcctgtta acacacgacg aggtgcaata taggtaactc ctgttgataa
     9841 gtcgcctgtg ttgaattcaa caccgtacga ttctttaaag acaggataaa gacttatgtc
     9901 tgaagataca ggtaatgctt gaatttctga ttcagaaagt ggagctccat tttgagtttt
     9961 agaccaacct acaaataaca atgttgaagt gggtggtgaa actttaaatg aacttaaatc
    10021 cgcggttccg tttcctgaac tatccaatgg tacttgataa cgctccaaaa tacttttacc
    10081 agcagaatca tcatataaag ttactgtcgc cactttactc ataaatggac gaacacaaat
    10141 ttcttttgtc tcagttacag ttattggctc accaaattta acagggtcac catactttcc
    10201 agtagtagga tcataggtat accaaccatt aaaatgctct cctgctttaa tcgtcggaat
    10261 cccaacttct cctagagatt ctccatcttt tataatttga tgatgaactt gcatacctga
    10321 agctgtcagg aaatcataat cagttccgtc attattttga aaatggtaag ttaacctagg
    10381 aacttctgta ttatcctctt gaacaattgc ataaatggag aatgaatctg ttttaaaagc
    10441 aacatcactg ctagtgttct tagtttctgc agtatcttta gattttaata cttctgtttg
    10501 accatcatct ttaaagtgaa caactttaag gttttcatct gaagcttcta atggcttatc
    10561 atagttaacc tctactttta ctggagcttg gggttctgct tctttaccat ttgactcaat
    10621 agtaatgtca tagagtttga aatttttgat ttcactatct tgtttagcaa cctctgtcaa
    10681 tgcttttttc ttataatctt taaaagtagc tgaattgtct tttaattccg tcacctttaa
    10741 atcagcattt ttaggaatct tagcttcttt ggtcaaagtc actgttacag tatagtctgc
    10801 acctctaaac atcaatggtt cttcacgata agcagcttcc tcagaagatg atgtttctgt
    10861 tacactagaa gcaggagtct ggggcttgct ctgctcaaca cttgattgag aactagatgt
    10921 tgatgaagtt acctggctag aatttttatt ttctaaagta atccccacat catctgtctt
    10981 agtttcttca actgttattg ctggtagaat taaaaaataa gtcgttaaaa aagttgttag
    11041 gatcatcaat gaccacatga taattttcca ctctttaggg tgttttttct tttttaatga
    11101 ttcgattata aaagtttgac acttctttag catttttgca tcctccctaa ccttaattga
    11161 tactactaat cttacctaga ggccatattc tgaaagaaat tttacctaca atttgttctt
    11221 ctgaaacatc tcctacagaa gtacttcgag aatcaattga agtttttcgg ttgtctccta
    11281 atacaaaaat ttttttatca ggtacttgat atgggtattt tatattacta ttaccgagtg
    11341 ctttatgaat aacatatggt tctttcaact tatgttgatt cacgtaaaca tccccttgag
    11401 aatcaatatt aacccagtct cctgactctg caataacccg cttgactagg accttattat
    11461 tgtagtaaaa cgcgacaacg tctccagttt taaaatttga accttttact gtaaagacta
    11521 catcacctgc acttaaagtc ttattcattg aatgtccgta gattcttaat acaggcaacc
    11581 ataaaaccgc aattaaaatg gctattgagg caaccgccat caagatgtat atggtatttt
    11641 taatgacact ccaaaagcgt ttctgataag ttacgcgatc cagttcttga gataatttat
    11701 ctgaactaat ctgtcttttc attgtctacg cctctcttat ctactaaatt ctttacgttt
    11761 tctaaatagg tatctgcagc taactgagca gtctcaaaaa taccactaag cattaaggaa
    11821 gcttcggcaa tagaaccagc tttgctaatt ttgatttttt tatcatctag cgcttcttta
    11881 agttgctgaa tctctttctc ttgtttttca ataagaagtt gctgttctaa cataatttct
    11941 agcaagtctt ttctttttaa tttttttaaa tcttccatcg cgattacttc cttaactgaa
    12001 ccttaaatta tcgtttagat attatatcaa agttctaacc tttaaactca ttttttgtcc
    12061 tgtgtttttt ctcaaaaaag tctatgctaa attaacattt ttgataattt tttgaaaaat
    12121 ctcatcgaag tcattttctt tttgaaagct cgaattctag gcattaaaaa gccatatatc
    12181 aaattgatat atggcttttt ttattattta aaacaaaaga atcaatagga caatagcgtc
    12241 aatttagtga cataatctat tacagattaa gttctttttg aataatataa tccaactttt
    12301 caactgtttt ttcccatgtg aaatgttctt taattctttt agcaatattc tgttgtagtt
    12361 tctctcttaa tgccttatct tttactaata aatcaagaga ttcatggagt gactgagtat
    12421 tttcttccat gatgattcct aactcagggc tatcaataac ttcaactgtt ccaccgcgat
    12481 ctgttgcaat aatagcactc gaaagtagac cagcttctaa aatagaggtt ggtaatccct
    12541 ctggatacat tgatgggtaa acaaagatat ctgtctgtgc cattaaagac atagtctgtt
    12601 caaagtttaa tttccccaaa aagttaatct gtttggactg atatttctct ttcaaatgtg
    12661 ctaattcagg tccgtctcct gcaatctgta aataaacatt ttcagagtac tgtgacatcg
    12721 aaaatgcttc taagagcaat tcaatgcctt tttctttaat aattctacca gcataagtga
    12781 tgaaaatatc atcagcagat ttttcaaggt aagccgtgtc agcaaaatca gaacctagac
    12841 tttcagatac cgaattataa ataactcctt tagcttctat attaaaatgt tttaaccact
    12901 caacgcttct cttagatacc gcataaaaat ctggacgata gtgcttaaca cgcgctgtga
    12961 gaagatgttc atagatagct ccaaagaaat ctaaaaaacg attattgaca gaaaaatgac
    13021 ttgacccatg gtctaaaaca atactaggta aacggtgctt ctttgcaaaa gatagccctt
    13081 ctagcgttgt taactgaaaa cgtgtattac aaatcacaaa atcaatattt tcatctgaaa
    13141 catgtttcat cagcgtgttg tattctcgat ttttgttaat aataggatag cgctgcttga
    13201 caatattttt ggtcggtaaa cggtaaattt ttctaccctt gtcttcatct ataatcggta
    13261 aatcatcatg attagttgtt acaataacaa cacggtagcc acgcttaacc aaatctgctg
    13321 tcattttatc tgtataacgt tcaatacctc cgaggaaggg tagataatat cctgagaaaa
    13381 cagcaactgt ttttacctta ttttccatat ttatccactt tcatcaataa gccatctttt
    13441 aagcctttaa tcatagcaac taattttttg ctcttttgct cttctgctac caacactcga
    13501 acaaattcat ttcgcataaa tactaaatat ttgtgccgct tcttcttacc atattttttt
    13561 ataatataaa tcgcattgcg tatcatgtaa tattttcgaa atggtgaatg attcaataca
    13621 tgaaaaacat ggccaaattt tttaactcgt gaagagtgtc caatttcgtg taaaagacca
    13681 ataaaattaa cctgataagt cttatatccc atctctgaca gacgataatt catttcagag
    13741 tcaacaaaat caataaacat ctcttctgca aagccagatg tttcttcaaa aacgctcgtt
    13801 ttcattaaag cagccgaagt aatacactct tcaatttctt tatagtcaaa ttcttgcata
    13861 actaaatctt cacggttcat atcttgatac aaacaagata acataccgac cttaggtaaa
    13921 agaaggtaat tttcataatt atctatcaaa tcacctagga caaccgaatc ttgatctaaa
    13981 gtcaagaacc aatcaaattc ttgtgctact gcaaattgac cgatacagtt caaagcatat
    14041 gcaattcctt tattttctgt taaataatca acagttaggt gcccctcttc attataatcg
    14101 gccactaatt gagaaatttc ttccttattt ttcgagccat tatctacgat gtagatatgg
    14161 cttacttgag gataaattgc tcgaatgttc tgatctaagc gttcaatatt ggggttaaag
    14221 gtgacaatac ccgctaaata tttcatgttc tatgctcttt tctaaaatct ctaaataact
    14281 gaatgactgg tgctttggtt ataaaaacga taccgacata gatagttact gctactaaac
    14341 tttgaatgac ataatttacc aatgatactg acatttgagt attgatataa taaagtacag
    14401 ctccactaag agtagcagca attaaatagc gcagcattcc tcttgttaat tctttaaaag
    14461 taaatacatc tcttaaagag atagcttgat agagggagac aataaattca gtaataactg
    14521 tagagataat agctcccata gcacctaaaa ttggtattaa aagtatatta agcacaacat
    14581 ttgccacaag tccaataact gcagacattg tgtaagcttt tgtacgtctt gaagccagta
    14641 gatactgtgt ccctaaagcg ttaccataag aaatgcaaat gatcatcaaa gactcaacag
    14701 ccattagcgg accgacagct tcataccctt tcccaaaaaa gaagacggca aatgtcgacg
    14761 aaactcctac cactcccgca atcattaaca ttgagatagc attgcttaaa tcaacaagtt
    14821 tcaaaagtaa agccttagct tgcttttctt ttcctgaaga aaatagactg gagagccttg
    14881 gtaagaaaac tcctccgatt gctgaaacaa tggtaaataa aatacgaata attttatcag
    14941 attgatcaaa atatccagca ctgaccacac tatccattgc tcccagcata ctcttgttaa
    15001 gagagagata aatctgcatg gtgatttggg gtaaaaataa cattaaagtt ggtcttagat
    15061 gtattaagat atctaatcta ctaaatggta tcttgatgat ttcatgcttt aaatgatgcc
    15121 agactgttaa atttccgagt aatgatgcaa atgcaattaa aaacatataa agtgctaggt
    15181 catctggcgt cttcacaact aagaaggtca aaacaactgt aataagctta acaatggtat
    15241 tacgaatgac aattatctta aagtcttcaa ctccaataaa ataccacgaa atatcagttg
    15301 cagtcgctaa taaattaata ccttgaatta aatagaataa ttgccattcg tttgtaagta
    15361 agacaaatcc aaagaataat aacgttgcta tagatacaga ggcaagtttt aatacaacta
    15421 attcccaaaa tatcttactt ctcttatact tatttgactg cacaaaagct atctcacgat
    15481 taccgtacaa agatatccct aaagcaccaa ataaagtaaa gtacatgaca atggagctcg
    15541 tataggcatt aataccgatg ccttgaggag atagtacacg ggaaacataa ggaactgtta
    15601 ctaaaggtaa caacaaagtc aataactggt atgaggtatt ataaaacata tttttcaaaa
    15661 gtttcattag tttctcctca taattaaact aataacagta tcaaataaca ataaggcatt
    15721 tgatacaatt accaagccag ttaacgcata cggcaccact ttccttcctg cctcatttag
    15781 acgcgctgga atacttgatg caaaaacttg taccaaaagc aatgtaacag taaagtaacg
    15841 tccttgaact ccttcaatgt agcttgttgg cgctccacga tattctaaca tgataaaaaa
    15901 gacaccaaga attcccacaa agaaactagc taagaaaaga aatcccatat tttttgactt
    15961 aacaaatttt tcttcagaaa accatactag aaagaccatg agataccata ctgatgatac
    16021 ccaagtggga ataaaattct gacttggata tggagaggca agccaggaag taatgtcatt
    16081 acttaaaata ttgataccag cataattata gccaaatgtc attacataac gtcttaaaac
    16141 agcaaggaca ccaccatgtc ttgcggtcag aacataaact atagcaagtg acaaaaaggt
    16201 tagacatagc caaaatagct ttttagattt aagaccttga aataaatgtg atagcttctg
    16261 attatagccc attaatttag gattgttaac ctgcaataat accggtatta ctagcaataa
    16321 aaacagccag taattctgct tacatgcaac aatgactaaa agaatcaatc ctataagagc
    16381 aatactacga tttttagtca acgatttatc aactaaaaga tttatcaaaa gcgataaaaa
    16441 tgctccaaat acaatatatc cagtcgagtc ataagataga ctagcaaatg aatttagaac
    16501 tacgggactg aggaaaatag caaagtagat taactttcct tttttcacat accggataat
    16561 gagggcaacc aaggtgattg agacaaagga actaaataac cttgctactg taatcattac
    16621 ccccatagaa ggatacacaa atttgcctaa aaataagcca actgctggca caagatgacc
    16681 ccaaaaaaca aagttatgtt tagaatagtt tatctcacga ggcaaatgat taccatcaac
    16741 aaaattggct ttatttaaat agtatttttc aaatctagta ccatctctat aggaggtcga
    16801 ttgtcgtctc atacctgaat caatattata ttctccatag taagaagtat caataatgtt
    16861 atttgaaata cgaccagaca cagctaaatg gtattgtcca tcaggttcat tgaaaaatgg
    16921 cattaatagg ctcaatacaa tagtaatcgt taagcctaat ataaaatatg ttttatgcaa
    16981 gttcttgtct agaaaaaact tttcaaatag acgaactttt ttgaggttca tattaactcc
    17041 tttttatatc ttttaataaa aacaccaagt cacgaatgcg atttgaaaag ctatcataga
    17101 accgtttttt agtcatcatt tgtttaagta ttgctacaaa atctttattt tgagcatttt
    17161 tataacgttg ctgattaaaa tccagataag ctttagcttc ccttaattga ctagagctac
    17221 ttttttctaa aaatggtaca aggatgatat agtaactcaa aacaaaatac agttcttgta
    17281 aatgcctttc gtaacttgag ctcatattca agatattccc acttgcattc tcagcatgtc
    17341 gcttatgttc accaacactt ctgtttaaat tgtaacctga accaatgaca ccagataccc
    17401 ctgcaaaaag attatcatgt gacaaaatta aattatcact gtaaaaagtc attacttgat
    17461 caacaaacga cttgcgtaaa caaaaagtcc aaccttgacg atagttatga tatgaaaagt
    17521 ctaatggata cttagaaatt gtttcctcac cattgaactt aaaattattg ggaatagtag
    17581 cctcttctga taactttttg atatcaatat cggcgctaag aaggtttata tcttggcgtt
    17641 cttccataat atcaacttgc atcttatttt tatgatgata ccatgtgtca tcttgatcac
    17701 taaaaaagac atagtcaacc tcataggcta acacatcaat aagtaattga cgaaaattta
    17761 aacgccaacc taaattttta tcattactag ttattgacca accatctaaa cggtgctctt
    17821 ttatatagtc ttcaaccacc tttacagtat catcagtaga gccatcgtcc ctcattaaga
    17881 catagtctgg tctcaacgtt tgatttctaa ttgaatctag ctgatcgtgg atgaaacctt
    17941 gaccattata tgttgccatc aaaactgctg tttttaccat attattcctt actttttaaa
    18001 cgcaaataat aaatctctca tacgtccact attagagtta tagagtcgcc aatttttaat
    18061 cacaaaagca ctattatgaa ttaatgacaa tttagaagct tccaatcttt ctttttcaaa
    18121 ggctaagcgt tgatttaaag cgacatcatt ttgatatttg ttgacaacct tctgcatggt
    18181 ttcgtaaaaa tcaacaaatg tttttaatcc tgtgatatgt tgtgttttag gcgacttcca
    18241 attcaaaaca aatttgctat taccacttgc gtttcctcca tatcgaatat gaactcctac
    18301 gctttcgtta aggttataac catttccaat aatactggca gttgtctcga taagattatc
    18361 gtgcgaaata ttagctccaa aagtccaatt atcagcgata gcttgcgcaa aatctctctt
    18421 catagcaatg gtccatccgg gacggaatgt ttgataatca tatactttag gatactgact
    18481 gattctatca ctagaagaaa aagttagaaa atgtggaaca ctggcttctg tagacatcgt
    18541 tttgatatca acgtctgcac tcaaaacctc aatttgaggg ttatctgaca taatggcaaa
    18601 ctgtcgttcg tttttatcaa gataccaaat atcatcttga tcactaaaaa agacatagtc
    18661 aacctcatag gctaacacat caataagtaa ttgacgaaaa tttaaacgcc agcctaagtt
    18721 tttgtcgttt ttaacaattt tccagccttc taactcatgt tttgcgatat agttattgac
    18781 gacattgact gtttcatccg ttgaacaatc atccctcaat aatacataat ctggttttaa
    18841 tgtctgttgg cgaattgaat caagttgttc agatataaat ttttcgccat tgtatgtcgc
    18901 cattaaaact gctgtcttta ccatattact cctcaaatgc ttgtactgtt tttaatattc
    18961 cagattcaag agaaacttct cgcttccatc ccaacgcttc taatttggcc atagtgagac
    19021 gtgttctccc cattttgcta taacctgcac tcatagtttt aggaatatcg aatactactg
    19081 aaatattttt atcagaaaag agatcaacta acttttgtgc caaatcctta atcataatgg
    19141 gttgatcttc atttgctaca ttgtaagctt gaccaacttc accatttaat aaaatagtaa
    19201 ataaccctga tacagcatct gctaaataac agaaagcacg ctctgctgta ccactacttt
    19261 ttaaaacgat atctttgcca tcaatgacat tcgataaaag atcattcata atacgaccat
    19321 cgttaccaag ctccatacca ggaccaaatg aatgtgcaat acgagcaata gtaaagggta
    19381 ccttatattg atcatagtaa ctttgcagta gagtttcagc catacgtttg ctttctgggt
    19441 aacaagctct agtagctaaa atatcaaatc caccataagc ttcttcatca atcacttctt
    19501 taatagatgt tccatatact tcacgcgttg ataagaacaa gaagttttca attgtttttt
    19561 cctttgcaaa gtctaataaa ttcaatgtac caattgtatt agcttttatg atgctaacag
    19621 gattactcaa aatagcagtt ggactagcat tgcttgctgc atggataatg taatccactt
    19681 ttccatcata ctctaggcgt tcttctacat cataaggaat taaggtgaaa taatctttcc
    19741 caactaaatc tgaaaattta tcacgggctt tttcaatgtt tctagctgtc gcaataattt
    19801 gcgtttggta atttcttgtc tcattgagat aggctagtaa aaataccata taactcgcta
    19861 acatactatt agaaccactt attaaaacgc ctttattagc aattctttcc caattaatat
    19921 aattttggtt actaataagt gctaaatctt cttgtacaat tttattacta aatttaatca
    19981 tcattacatc ccataaattt gttcattttc tctagcatca tacaaagctt taaacatata
    20041 aaaatcatct ggtgttgtta tcttgatatt ttcatatggt ccctcaacaa tagtgagttc
    20101 acgattgtac atacccatca aagtactaga atcaattgca tcagtaatcc ctttgctaat
    20161 ggcatcacgt tcaacagaaa ggatatctga tagataaaaa ctttgtggcg ctttagcaat
    20221 gaaggaacga gtacgatcaa caacttcaga tattttagaa gaatcattga ctagaacaac
    20281 tgtctcttta gcacgaactg aagttaccgc acttcctgtt tcttttactg actgtatatt
    20341 agcattaata acttcttcat taatcaatgg acgtactccg tcatgaatta aaacaacggc
    20401 atcatctgta gccagctgtt cagcggcttc aagcccctta aaaattgaca tttggccagt
    20461 ttctccacct gcaacaactg cttttacttt cgtaaggttg aaacgctcta ccagattatt
    20521 catataatct agccagtctg atacacagac aactactaca gcatcaatag cttctgtatt
    20581 ttggaaaata tcaattgtgt gtacaataat gggtttacca tgcacttcta ggaattgctt
    20641 tggtttacct ttggtattca tgcgacgacc aacaccacct gcaaaaataa ctccaatatt
    20701 catcattaac cttctttcca gtaccaagtg taataactat gactttctct cttactttct
    20761 ggaggtaatt ccatataact acccccatat aattgattca gataagcatg ctcattttta
    20821 atttttcggg ttttaatgtt ttcaaacata acatcttcat actcagaaaa aatttctctt
    20881 ggaaaatatt ctttcaagcg atatcttgaa tcggtataac ccatgtaagg aacctcttga
    20941 ttattatatg tttccatcac ttgatcaaca atttcagcac gttttttagc ttggccgtga
    21001 taagcgatga atcggggtag ccccaaaatt agccttgcat ttgcataaag ctttcgggaa
    21061 ccgctagcaa aattggggta agtcgtacac atcaaatttg cagcagcaga gtgaactttt
    21121 ttaaagaatc tttgacgttc ttctgcactc tctggtaatc ggtcaagtgg gaagatatca
    21181 ataaaaatcc ctgtccaagg atgaattcta tctgttttac ttaagtacat tgactttgtg
    21241 tgatacagct tggcaaatgg ccatagattt ttttcaacag aatgatgtag caatttaaaa
    21301 tcgctattat tagcatcagc taatgctttc atcaaacgct catactggct tctttctagc
    21361 atcaaatcaa tatcgtcatc ccatggaatg aatcctttat gtctcattgc tcccagtagg
    21421 ctgcctccgc ctaaagaata ttcaatttta ttatcacgcg caactttatc aatataagct
    21481 aacatttcaa gttgaacttc tcttatttct gagaccgtca tttctttcat aattacctct
    21541 gtctaacagt taactcacat tcttttaaat acaaatctta tagctgtaat atagaaaata
    21601 aataaagcta ctgcaaaaag aatcactagg gtcatgggtt tgaaagtttt ctttgttttt
    21661 tgaccttttg gagtgtttgc aagtgcattg tattcttgtt tgttgtactg catgtccatt
    21721 atataaccat ttatattata gttaccaaat tgtgctttag aaacactatc tttatttact
    21781 gtaaaataaa atggtttatt gctttgtaag tagccttttg gagattttgt ctgtattaag
    21841 aaatacttat acttattaga caaacctcgt aatttaaata atgattggtt acttttcact
    21901 tgttttttat agttataagc ccataaccta ggattcaagt ctatgtcatt tgaatagcca
    21961 tcaataccta taatgttatt tcctctaata tggtaagcat tttggttaag atattcttta
    22021 tttaaactct taatcgtttt ttgagtattt atccaaaagc cattaggttg atatagagat
    22081 acttttttat ctgcattcaa tcttcctaaa atatactctg aaccttgcaa taatctattt
    22141 tgattgtcgt catacatggc aaaatttata ccataactgc tcaaactagg tgtctttata
    22201 tagcctttag aatcctcgac aaaaatccct tgatcttgtt tttgattatc aacagaaatt
    22261 agactccgtt tagtctctat gacgcctttc ttttcctgag caacagacaa attataatct
    22321 ctttttgaag caacatgtcc tgtaatctct aactctttaa tatcagcatt tgtcaaatca
    22381 aagtctaact tatcaataga gtgaagttta tacaggctat tttgtaataa ttctttttgc
    22441 tctgtttcat caccactact atttaaaaca attttatcac catcgacact aatactgcta
    22501 tctattggat acaaacgtcc ctctttacct tcttccgcaa taataggcgc ttgagaaagt
    22561 cggtaattat aattagtact atctactaca aaattagggc taactctgac agtcagctgc
    22621 tttgaataaa ttggaatcct ataagtaata cgtttaccgt attcaactcc tatagtatct
    22681 ctttttttgt tattaacagt tactcgagga ttatctactc gggtattacc aagataaatc
    22741 gtttcgtttt tctgaatatt tttcaacaca tattcttgag tttctgcgct gttttcaaaa
    22801 gcagttaaac caactggaat attaccaatt gcttttcctt tgttatttgt gaacttataa
    22861 cgtttatttt ctggattata taaaacactt ggctcttcta agccattcat gtaaattaaa
    22921 taatctttat tcttttcaag cagtaacctt acaatagcaa tagcatcctg tctagtattt
    22981 tttataccag aaccaaatct atagttatta tccataagga cttttttacc actaacttgg
    23041 tacttccgct tgatactatc aaaagtatag tgtgtaaagt ctttgctctc aaactccccc
    23101 gtcctcactt tatccaatgc tgtttgtaaa tgaatagcat taatctgaac actgtattgc
    23161 ggggtattat tatgataata ataagctgta atatctactt ttttatctgt ttttactttt
    23221 ttattatttt ggcaacctac taaaaaaagc atgaaaaaca gacaaataaa aatatgtttt
    23281 ttactcatct aaagtgctat cctctatctc ttttttcaac aaagaaacct cttgaatcag
    23341 gcgtttaatt tgctcatctc ttttggtatt gctactagta tcaaataatt ctaaacaaaa
    23401 cagaaataaa atagcaccaa ataacaagaa attcatcgtt aaagcgaatc caaggtgatg
    23461 agcgataaat gagaccaccc caggaaatac actaataaag actagaatta aaccaactaa
    23521 tagccaaggt aaagaacgtt taacagaaaa tgtgttttta ttaaccattc ttataatcat
    23581 ataaagtagg acaatggcta aaataaacat ttcaatttgc aatgctaaac tcatttatca
    23641 tcctccttaa taaaccctgc aataataatt gatgttaaaa catccgtcat ataaaaaatc
    23701 gattttaggg aacttatact tgaagtacct gcctcacgtt caaacatatt agctgtgact
    23761 tccttcaaac gatagccttt tttcaaaatc cgagcagttg attcaggctc tgggtattgt
    23821 actggataat gacacgataa ataagctatt agcgccgcgt tggctgccct ataaccactt
    23881 gttgtatcat agactttgtg tcccgtcgtt aattttatag cagcagatat taaatttatg
    23941 ccaagacgcc tcattgctgt cgattgaaaa ttttgatgtt ttttatcaac aaagcgagaa
    24001 ccaataacaa aatctgcttc gtcatttaag attggcataa caacatcttc aatgcttcta
    24061 atatcatgtt gaccatcacc atcaaattga acggcaacat cgtaatgatt acgattagcg
    24121 tataagtatc ctgtctgcac acagccacca atacctagat tttgaacaag gtcaatatga
    24181 tttaaaccca atctatctaa taactctgga gtaccatcgg ttgacccatc gttaataaca
    24241 atataatcca gctcaaaagg tagtgagcga gacgccttaa aatctacaat agtctggact
    24301 gtctttgcaa tactaccttc ttcattataa gcaggtatca ctagtaaaat ttttttcatc
    24361 atcttatctc ttattgtaat aatttgttgc aatcaaccat ttaaacacaa cttgatgctt
    24421 aaattgattt ttgctataac catatttcca taaccacctt aatcttgtca taaaaggctg
    24481 tttgtcaatt tttataaaac atttaatgag ctcaagatca ttagctgtca taatatcacc
    24541 atacttatca acaattaagg aagcttgctt ttgtgagtca tggataagtt tccaatacct
    24601 tgtaaatata gatttaggac cttcacgaag tatcttaaat ctcttatcca ttgtcctcgc
    24661 ccccaaaaca ttattgtcat gttgacggta tagttgagtt ggtaaatcca aataaatgat
    24721 ttctcctaaa cttgctgcta agagtgctaa aaaccaatca tgcattaaga tatcattagg
    24781 agtaaaccac ttctcagcta atgcatgatt tatcatcata gtcccaccag taacagtatt
    24841 ctcagttagt tctggtaata atgtcgtatt ggcatggtgc gattgagccc gaatcatgct
    24901 gtcttgtaaa atatttaatt cttgattaac aactttcaaa tcagtataaa caagtaatgg
    24961 tacatcagat gctttatgct ttgctgcctc taaacttaca gataactttt ctggcaacca
    25021 tacatcatct tgatcactaa aaaaatagaa atctgcaact tcgtaattaa ctagcgtaaa
    25081 aaatgattta ataactccta aattatggtg ctcattttca ttaatgaaac gaattcgaga
    25141 atctttagca gtaaagtttc taataatatc acatgtcttg tcacttgaac catcatctct
    25201 gattaggagg ttccactctt taaaggtttg cttttggata ctttcgattt gctgcgccag
    25261 aaacttctct ccattgtagg tggccattaa aatattaact ttcatttaaa aacaaatcct
    25321 catattcttc aacaattttt ttccaagtat agttttcttg aataatggct tttgctcgat
    25381 caccgtaagt agataaatcc tgttgttgat cactatcgtt aataagctta gctaaatccc
    25441 cctctttttt agtccagtaa aaacttgaga gacctgctac tgatttgttg aaatcaacat
    25501 ctagaacaag attcaaatca gtattagcta aagcctcaag cagtcctgga ttagtaccgc
    25561 caacttcatg cccatgaata taagcaaagg cttgttgacg aacataatcc agcagatctt
    25621 tttcatagag cgtacctaca aacttaactc ttttatcttg ttgaaggttt gtctttaagg
    25681 acaacttttc aaagtagggg ttattttgat ggttacagat aataactaaa tcacgcttag
    25741 tatctgaagc catgaactcc ctaatagccg tttcataatt gttttcaggg acaaatcgac
    25801 caacgattag atagtaaccc ttagacttaa tattccattt tttaaacaac tgtttgacac
    25861 gtggatcatc tgacgataat ttcctagaat taatctctgt accgtaagca atgaaccttg
    25921 tcttcacatt agagtaggta gattgaatgt aattttcaat accaatatta tcagaaataa
    25981 ttaggtctgc atttttagtc atacattttt cggcgtattt taaataacgc tgtgtgggac
    26041 gagaccactt tgatcgcttc cactctaaac catccggatt aacatacaat aagccaccga
    26101 ctttatgtat tttattggca aaatgccaaa taaaggcacc aattgtattt cctaaaatat
    26161 aaaaaatagg ctcttttaaa tcatgtgtct taaccaagtc aagggcataa ttaatggcca
    26221 taatatcata agcaatcaca cgtgctggcc ctaattgggg aggatttata gtaaaacaat
    26281 cagcgtcagc aaagttaaaa tgagtatgat gttctttatc actaaggcat gcaacatggt
    26341 attttatgtc ggaacttttt tgatgattaa tcaattctga aacaaaagtt tcaaaaccac
    26401 cgtaacgagc tggcaaccct ctacttccta taatgaaaac atcttgcatc atcttctcca
    26461 ttctagctaa acaatccata caattatacc ataaattaga aaagttagct aaaaagagca
    26521 gtaagctaaa aggattctct agccttacta ctctttgttt tgatcttatt ttttaacttc
    26581 ctgcttgtag aactctttta aagcatcttg ccaagtagga atcacaaatc ctgttgcttt
    26641 cgctttttcc aaactcatag ttgaatttaa aggacgtttt gcttttgctg ggaattgact
    26701 agaatcaaca ggcttaacct ccacatctgt gtcttttaag atttcaaccg caaaatcata
    26761 ccatgttgta tcttctttag catcatttga taaatgatag taaccaaaat ctttttgatt
    26821 ttcagctaaa taagtcataa actcagccaa agtacgtgtc catgttggtc gtccatgttg
    26881 gtcgttaaca acagttagag ttttgtgtgt ttttgcaagg ttttgcatcg taaagacaaa
    26941 gtttttacca tagttaccaa atacccacgc tgtgcgaatg gtatagaatt ttgaagtata
    27001 tttttcaact aactcttcac ccatacgttt tgtacgaccg tactctgttt tgggatcagg
    27061 cagatcatct acttcccatt cttgccccac tggtttttca ccatcaaaca cgtagtccgt
    27121 cgaaatgtaa acaagtgttg catcatgttt agcagcagcc ttagcgacat tctctgtacc
    27181 agtgacattg attgcaaagt ctagctcttt tccttcatct tctgccgcat caacagctgt
    27241 gtaagctgca caatggtaaa ctaaagatgg cttcacctct tcaaagactt tgtccaccat
    27301 ctcggcattt gtaatatcca tttcagctac atcaacagca acatactctt gagttcgttc
    27361 gtctaaaagg tgacgtaatt cactacctaa ttgcccgttt gcccctgtaa ttaaaatcat
    27421 tccttgctcc tttaaaaata agtttaactt ttaattcttc gtttattgta cactaaaaaa
    27481 gggaatattt catcccttta ctctttatct tatgaacgaa ttcccaaagc gatacgagca
    27541 taacgactca tcttatcaac agaccaggct gggtaccata ccaatttcac ctcggtttca
    27601 gttacctctg ggactgtttt cataacatca tgaatttgat ctgtcaataa atctgctaac
    27661 gggcatccca tagtcgttaa agtcatatca atttcagttc gaccgttatc ttcaaagcga
    27721 atctcataaa ttaacccaag gttaacaata tctattccca attcaggatc gataaccatc
    27781 tccaaagctt ctaggatacg atcttttatt tttccaactt cttcttcgct atattgcttt
    27841 acttctgaca ttataatacc tctctgaaaa tattaatacc gtctttaaga taactccttt
    27901 gcaaatcatt tttattagaa actaaacttc ctattctaaa aacgattatc gagaagcaat
    27961 caatcttcca tgaaatcttt aagttgcttg ctacgactag gatggcgaag ttttctaagg
    28021 gctttagctt caatctgacg aatgcgctca cgagtaacat taaatacttt acctacgtcc
    28081 tctaacgtac gcattttacc atcgtcaaga ccaaagcgta aacgaagaac attctcttca
    28141 cggtcagtaa gagtatctaa aacctcatct aattgctcac gaagaacaac acgtgtagta
    28201 taatctactg gattttcaat aacttcgtct tcaatgaaat cacccaaatg gctatcatct
    28261 tcctcaccga ttggcgtttc tagcgatacc ggctcttgag caatttttag gatttcacgt
    28321 actttatctg gtgtcatatc catgcgttct gcgatttgct caggagttgg atcttgacca
    28381 agttcttgaa gaagattacg ttgttcacgg accaatttat taatcgtttc aaccatatga
    28441 acaggaatac gaatcgtacg tgcttgatct gcaattgcac gtgttatagc ttgacgaatc
    28501 caccatgttg cataagttga aaatttgaat ccctttgaat aatcaaattt atcaacagct
    28561 ttcatgagcc ccatgttacc ctcttgaatt aaatcaagaa attgcattcc acgtccaaca
    28621 taacgttttg caattgagac aacaagacga aggtttgctt cagctaaacg ttgtttagcc
    28681 attaaatctc cctcagcaac cgcaacagcc aattcttttt cttcttcatt cgttaaaaga
    28741 ggaacaacac caatttcctt caagtacata cgtactgggt cattgacttt agcagaatta
    28801 cttcctatga gctcttcatc agtcaattct tcaggttttg gcccttctac cacatacttt
    28861 gttgaaggat tgccttcttt atctgtgatt gaaatgccac cgtctgtcaa tcgttctaac
    28921 aaatcatcaa tttgatctgc atctaaaaca aaaggaataa caagtttttc tgttacctca
    28981 tcatcaattg cagtaccttg ttttttatga ttgcgaataa aatccgcaac ttgaacatta
    29041 aaagttgtat ttcctttttt ctctgccata ggttcctcat tccatatttc ttttttgagc
    29101 aattaaattt tctaatgcag ccaaggctcc ttcttcatca ccttgattac tagattgtct
    29161 aattaactgg ctttgttttc ttaaatcacg ttctttcaat aaacggtcac gtgccttttc
    29221 gacggcttca atctctccaa tagatacttc tacaggtaat tgttcctcta aaattatata
    29281 ataagtacga ttaactttgt cactttcttg tgataaatca taagacgtga tctcaccact
    29341 ttcttttagc aactcatata aaacttgtat ttctgttgta tcaaaataaa aattatccct
    29401 attacggaat tcatttaaca ggtagggatg attcaataat cgatgaaaca attgcatttc
    29461 tgttttagtg atagctgata acttacctgt ctgtggtaat tgcactgtag cacttgtttg
    29521 tactgaagat gaagcttgct gttgacttct atgatgcaat cgttcgttat taaccgattg
    29581 ttctacttga aaataatcaa aatctggcaa gagttcagcc acttttgtaa tatatgaatt
    29641 ttgtgctgtt atcgatggag atttagcaat taatttcgct attttttcaa cataagcaat
    29701 ttcagattgt aaattatcgg tgttttcagg ctttaagtag tggatataaa actctgtatt
    29761 actgatgcgc ccattttcta agagttgttt aaaatcttct gctgaatttg cttgtagaaa
    29821 ttcatcaggg tccattttat taggtatcct aacgatatca acagtcatat ctgatagcag
    29881 ttctaaagac ttgtcaattg cgttttgacc agctctatca ccatcataag tcagaacaac
    29941 tttcttagta aatcttttaa ggtgtctaac gtgttcattg gttaaagcgg tccccataga
    30001 agcaacaaca ttctcaattc ctgcacgata agctgcaata acatccataa agccttccat
    30061 taggtaaact tcgtgtgctt tattaattac agcacgcgct ttatctaaat ggtaaagctc
    30121 gtaagactta ttaaaaatag gggtggcacg agaattttta tatttagctt gttttctatc
    30181 aatgtcctct tgagtccaaa ctcgtcctga aaacgcaatg acttcaccaa tatcattagt
    30241 taacggaaac ataattctat tattaaatga atcgtaaaac tgattgctat tttctgaata
    30301 attgaacaaa ccagaagaca tcaaagtttt ttcatcaaac tcttttgcca gacgacgata
    30361 tagaaaatcc tgccctcccg gcgataatcc tatttgaaaa tgtttaatta agtcctctgt
    30421 cactccgcgc tcggcaaggt attgtctagc gcgttcacca tcattagttg tcattaaata
    30481 ggcatggtaa aatcccgaag caactctatg aatatctaaa agcgactgat tagctttagg
    30541 ttttttagga acttgcccct taaaattagt atctagaggt attcctgaac gttcagctag
    30601 aacttgtaca ctatctaaaa aactaatatg ttggtaatct tccacaaatt taaagacatc
    30661 accagagcga ccacaaccaa aacaatgaaa aaactgccta tcttcgataa cattaaaaga
    30721 tggcgttttt tctttatgaa atgggcataa accaagatga ttgcgtcctg tttttgtcaa
    30781 accgacaact tcaccgatga catcaacaat gttaacactg ttcttgattt cagaaatttt
    30841 ttctttatca attgccaagt catgacctcc acagaaataa cccattatag ttttacattt
    30901 gattatatca ttttttcttt tctgtgtaaa ttttt
//
LOCUS       DATRSG010000016        30919 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRSG010000016 DATRSG010000000
VERSION     DATRSG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30919
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.016"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            171..1361
                     /locus_tag="VBD89_000725"
     CDS             171..1361
                     /locus_tag="VBD89_000725"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO7135275.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWQWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            1380..2768
                     /gene="argH"
                     /locus_tag="VBD89_000726"
     CDS             1380..2768
                     /gene="argH"
                     /locus_tag="VBD89_000726"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO7135276.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLEKMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            2908..3789
                     /locus_tag="VBD89_000727"
     CDS             2908..3789
                     /locus_tag="VBD89_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO7135277.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(3874..4791)
                     /locus_tag="VBD89_000728"
     CDS             complement(3874..4791)
                     /locus_tag="VBD89_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7135278.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            5030..5218
                     /locus_tag="VBD89_000729"
     CDS             5030..5218
                     /locus_tag="VBD89_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO7135279.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            5350..5715
                     /locus_tag="VBD89_000730"
     CDS             5350..5715
                     /locus_tag="VBD89_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7135280.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            5715..7379
                     /locus_tag="VBD89_000731"
     CDS             5715..7379
                     /locus_tag="VBD89_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO7135281.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            7527..8411
                     /locus_tag="VBD89_000732"
     CDS             7527..8411
                     /locus_tag="VBD89_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO7135282.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(8503..8619)
                     /locus_tag="VBD89_000733"
     CDS             complement(8503..8619)
                     /locus_tag="VBD89_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO7135283.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(8987..9217)
                     /locus_tag="VBD89_000734"
     CDS             complement(8987..9217)
                     /locus_tag="VBD89_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135284.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFIYSRG
                     YAPSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
     gene            complement(9559..10299)
                     /locus_tag="VBD89_000735"
     CDS             complement(9559..10299)
                     /locus_tag="VBD89_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7135285.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            complement(10309..11859)
                     /locus_tag="VBD89_000736"
     CDS             complement(10309..11859)
                     /locus_tag="VBD89_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7135286.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            11995..13878
                     /locus_tag="VBD89_000737"
     CDS             11995..13878
                     /locus_tag="VBD89_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO7135287.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKIIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KDHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQNQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHRFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            13924..14763
                     /locus_tag="VBD89_000738"
     CDS             13924..14763
                     /locus_tag="VBD89_000738"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO7135288.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            14883..15638
                     /gene="mecA"
                     /locus_tag="VBD89_000739"
     CDS             14883..15638
                     /gene="mecA"
                     /locus_tag="VBD89_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO7135289.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            15640..16800
                     /locus_tag="VBD89_000740"
     CDS             15640..16800
                     /locus_tag="VBD89_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO7135290.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLVMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            16965..17735
                     /gene="sufC"
                     /locus_tag="VBD89_000741"
     CDS             16965..17735
                     /gene="sufC"
                     /locus_tag="VBD89_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO7135291.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            17772..19034
                     /gene="sufD"
                     /locus_tag="VBD89_000742"
     CDS             17772..19034
                     /gene="sufD"
                     /locus_tag="VBD89_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO7135292.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            19036..20268
                     /locus_tag="VBD89_000743"
     CDS             19036..20268
                     /locus_tag="VBD89_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7135293.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            20255..20698
                     /locus_tag="VBD89_000744"
     CDS             20255..20698
                     /locus_tag="VBD89_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO7135294.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGNQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            20798..22216
                     /gene="sufB"
                     /locus_tag="VBD89_000745"
     CDS             20798..22216
                     /gene="sufB"
                     /locus_tag="VBD89_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO7135295.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(22287..23474)
                     /locus_tag="VBD89_000746"
     CDS             complement(22287..23474)
                     /locus_tag="VBD89_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7135296.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTNLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKLGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIASLCLLAGIVLLIK
                     RSR"
     gene            complement(23627..24862)
                     /locus_tag="VBD89_000747"
     CDS             complement(23627..24862)
                     /locus_tag="VBD89_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7135297.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIAAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
     gene            25104..26762
                     /locus_tag="VBD89_000748"
     CDS             25104..26762
                     /locus_tag="VBD89_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7135298.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKNN"
     gene            26877..27791
                     /locus_tag="VBD89_000749"
     CDS             26877..27791
                     /locus_tag="VBD89_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135299.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     ITLLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            27801..28832
                     /locus_tag="VBD89_000750"
     CDS             27801..28832
                     /locus_tag="VBD89_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135300.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRVVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            28845..29894
                     /locus_tag="VBD89_000751"
     CDS             28845..29894
                     /locus_tag="VBD89_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135301.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTETESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKAQ"
     gene            29891..30823
                     /locus_tag="VBD89_000752"
     CDS             29891..30823
                     /locus_tag="VBD89_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7135302.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNKPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT        10034 a         4970 c         6097 g         9818 t
ORIGIN      
        1 aactaccttt atcataatta tttaaaacaa taactgccaa ttggcagtta ttgttttatt
       61 tgtttagaaa taaatatact aaaaatagac aaaaactata aaaatatttg ataataaata
      121 tagcaagtgt ataatattac aaaatatagg aatttggaag gggatttttt atgggaaaag
      181 aaaaattaat tttagcttat tctggtggtt tagatacctc tgttgctata gcttggttaa
      241 aaaaagatta tgacgttatt gctgtctgta tggatgttgg cgaaggtaaa gatcttgatt
      301 ttattcacga taaagcttta acaataggtg ccatagaatc ttatatttta gatgttaaag
      361 atgagttcgc tgagcatttt gttttaccag ctttacaggc acatgcgatg tatgaacaaa
      421 aatacccgct tgtttcagcg cttagtcgtc caattattgc ccaaaaatta gttgaaatgg
      481 cacatcaaac aggtgcgacc acaattgcac atggatgtac aggtaaagga aatgaccaag
      541 tgcgttttga agttgcaatt gcagcactag atcctgaatt aaaagtaatt gcccctgttc
      601 gtgagtggca gtggcatcgt gaagaggaaa ttacttttgc aaaagctaac ggcgtaccta
      661 ttccagcaga tttagataac ccttattcta tagaccaaaa tttatgggga cgtgccaatg
      721 aatgtggtgt gcttgagaat ccttggaatc aagcgccaga agaagctttt ggtattacaa
      781 aatcaccaga agaagctcct gattgtgcag aatatattga tattacgttc caaaatggga
      841 agcctatagc tataaacaat caagaaatga cactagcaga tttgatttta tcgctaaatg
      901 aaattgcagg aaaacatggt attggacgca ttgatcatgt tgaaaatcgt ttagtcggta
      961 tcaaatcacg tgaaatttat gaatgtcctg cggcaatggt actgttagct gctcataaag
     1021 aaattgaaga tttaacatta gtccgtgaag tgtctcattt taaacctatc cttgaaaatg
     1081 aactatcaaa tcttatttat aatgctttat ggtttagtcc agctacaaaa gctatcattg
     1141 cctatgtcaa agaaacacaa aaagtggtta atggtactac aaaagttaag ttatataaag
     1201 gttctgccaa agtcgttgct cgccactcgt ctaattcatt atatgatgag aacttagcaa
     1261 cttatacagc tgctgataac tttgatcaag atgcagcagt tggttttatc aaactctggg
     1321 gacttccaac ccaagttaat gcacaagtaa acaaaggata aataaaggaa gataagagga
     1381 tgacaaaaaa tcataagtta tggggtggtc gttttgaaag tagtcttgaa aaatgggttg
     1441 aagagttcgg tgcatcaatc agtttcgacc aaaagttagc tccctatgat atgaaggctt
     1501 ctatggccca tgtcacaatg ttaggcaaaa cggatattat tagtcaagaa gaagcaggct
     1561 tgataaaaga tggtttaaaa atactgcaag ataaatacag agcaggtcaa ttgactttta
     1621 gcatatcaaa tgaagacatt catatgaaca tagagagctt attaaccgct gaaattggag
     1681 aagtagcagg taagcttcat actgcacgtt cacgaaatga tcaagtggct acagatatgc
     1741 acctttattt gaaagataaa ttgcaagaaa tgatgaagaa attgcttcat ttacgaacaa
     1801 ccttagttaa tttagcagaa aatcatatct atactgttat gccaggatat acacatttac
     1861 aacatgctca gccgatttca tttggacatc atctaatggc ttactataat atgtttacgc
     1921 gtgatacaga gcgattagag tttaatatga aacacaccaa tctatcacca ttaggagcag
     1981 cagctctagc agggacaaca tttcctattg accgccatat gacaactcgt ttattagatt
     2041 ttgaaaaacc atatagtaat tcgcttgatg ctgtctcaga tcgtgacttt atcatagaat
     2101 ttttatccaa tgctagcatt ttaatgatgc atttatcacg tttttgtgaa gaaatcatta
     2161 attggtgcag ttatgaatat caattcatca cattatcaga tactttctct acaggatcat
     2221 ctattatgcc tcaaaagaaa aatcctgata tggcagaatt aattcgaggg aagacaggcc
     2281 gtgtttatgg aaatttattt agtttactaa ctgttatgaa atcattgccg ttagcttata
     2341 acaaagattt acaagaagat aaagaaggga tgtttgatag cgtagaaaca gtcagcatag
     2401 ctattgaaat catggctaat atgcttgaga aaatgactgt gaatgagcac ataatgatga
     2461 cctcaacgga gactgatttt tcaaatgcaa cagaattagc agattattta gcaagtaagg
     2521 gggtaccttt cagaaaagca catgaaattg taggtaaatt ggttttagaa tgcagtaaaa
     2581 atggttctta tttacaggat atacctttaa aatattatca agaaatttca gagttgatag
     2641 aaaacgatat ctatgagata ttaactgcaa agacagcagt taaacgaaga aattcattag
     2701 gaggaacagg gtttgaccag gttaaagaac agatcctgtt ggcacgtaaa gaattgaaag
     2761 cggaatgact gctttctgaa aaaacctcgt ttaactctgt taagtttctc acgtattttc
     2821 agtaaaaaac tagcattttt tatagaattg tgctagaatt aacatgtaag tgggcaacgc
     2881 ccaaaaaata aataggaggc ctgaataatg gcaatcgttt cagcagaaaa attcgtccaa
     2941 gcagctcgtg ataacggcta tgcagtaggt ggatttaaca caaacaacct tgagtggact
     3001 caagctatct tgcgcgcagc agaagctaaa aaagctccag tacttatcca aacttcaatg
     3061 ggtgcggcta aatacatggg tggttacaaa ctttgtaaac aacttattga aactttggtt
     3121 gaatcaatgg gtattactgt accagttgct atccaccttg accacggtca ttacgatgat
     3181 gcccttgagt gtattgaagt aggttacact tcaattatgt ttgatggttc acaccttcca
     3241 gttgaagaaa accttgaaaa agcacgcgaa gttgttgcta aagcacatgc taaaggtatt
     3301 tcagtagaag ctgaagttgg tactatcggt ggtgaagaag atggtattgt cggtaaaggt
     3361 gaattagcac caatcgaaga cgctaaagct atggttgaaa ctggtattga cttcttggct
     3421 gcaggtatcg gtaacattca tggaccatac ccagcaaact gggaaggact tgaccttgat
     3481 cacttgaaga aattgactga agctgttcct ggtttcccaa tcgtgttaca cggtggttca
     3541 ggtattcctg atgatcaaat ccaagaagct atcaaacttg gtgttgctaa agttaacgtt
     3601 aacacagaat gtcaattagc tttctgtcaa gcaactcgtg actacgctgt tgaattcaac
     3661 gctaacgaag ctgaatacat gaagaaaaaa ctctttgacc cacgtaaatt cttgaaacct
     3721 ggtttcgatg caattacaga agcagttgaa gaacgtattg acgtgtttgg ttcagcaaac
     3781 aaagcataat taacataaaa cagataatag ttaaaagctc acttatggtg agcttttttt
     3841 ggattatatg actttttatt aaatagatac tctttaaaga ttgatatgct taagataatt
     3901 tcgaataaat gccgctgaat ctgtgagttg ttgctcttct tttgggctta gtgaaagaga
     3961 aattctttct acaattccta ctctaccaat aatagctgga tatccaaggt aaaccccttc
     4021 ggactcatag aaggtagata caggtagtaa ctcatgggaa tcatttaaca cagctaaaac
     4081 gatgcggaca gctgcggtag cgataccata actagtatat tttttaccaa aaaagacagt
     4141 gtgcccgcct tttaaagaag cttcttttat ctgttctaat tcttctactt ttaatagtga
     4201 tatagctggt tgtcctttta cagttacctg actccaagcg acaaattggg agttaccatg
     4261 ctctcctaga ttataaccac cgatactcct tggatcaact tgtaggcgtt gaccaagcac
     4321 tcttttcatc cgagcagtat ccaataaggt tcctgttccg ataacgcgtg atttaggaaa
     4381 ttgagtatat tcttgataaa gctgggtgat ggcatcaaca ggattagtaa cgacaataag
     4441 gatccctcta aaaccagatg attttaactt ttggctaacg tcgtagactt ctttactggt
     4501 aaatggtagt tcagcaaagc gattagcttc tttgttattc tgtagtgaaa tatcacctaa
     4561 ggctgaaatc acaacatcag catctgacaa cgcgtcatag tcgtttaata ctatattagc
     4621 gtgataagta gcatttgcca gagcatcttg aaaatcaatc atatcagcat taaccttatc
     4681 aacattagta tcaataaaga cataatcatc accaatacct tgtgcaataa gagcgtgtgc
     4741 aacagttgat ccaacgtgcc ccatgcctat aattccaatt ttacgtgtca tatgaaacct
     4801 cctttgatta ttgttgtcta ttataatatt ttgaaagaaa atgtaaacta aaaaatgaat
     4861 tagaaggaag tgtgtcttat tataaaaaat atgtaagcga tttagataaa acaaatgaaa
     4921 aagtaatttt gaaaagaact tgctagaaga agtaagttgt gatagaatag tctagtatgt
     4981 ggtgctagca catccgtaaa tattgatcaa tataggagga actcgtaaaa tggctaaagt
     5041 atgttatttc acaggacgta aaacagtttc tggtaacaac cgttcacacg cgatgaacca
     5101 aacaaaacgt actgttaaac caaaccttca aaaagttact gttcttatcg acggtaaacc
     5161 aaaaaaagtt tgggtttcag ctcgtgcgct taaatctggt aaagtagaac gcgtttaata
     5221 aactgagaaa gccttagggc tttttttagt gtttctaagg ctgatttgat agttagatac
     5281 ctctattttt ttacaataat agtgagatat gatagaatag agtgataaaa actttttgag
     5341 gtagtagata tgactgtaaa aatcaataca agagatggtc aaattgaact ctctgatgac
     5401 gttattgcaa ctgtcgttgg aggttcagca acggaaattt ttggcgttgt tggtatggca
     5461 agtaaaagtg ctatcaaaga taactttcaa gcacttttgc gtaaggaaaa ctacgcaaaa
     5521 ggtgttgttg tgaggtctag tgaagcgggc atcgcagtag atgtttatac tgtcatgagt
     5581 tacggcgtca aaattagtga agtttcaaaa aatattcaag agcgagtgaa attcaatctc
     5641 gaaaatcagc ttggtttatc tgctgatatg gtgaatgttt atgtgcaaag tattaaggtt
     5701 gtaggagaag attagtgtca aagattacga caagtttatt ccaagaaatg gtacaagctg
     5761 ccagtacacg tctgagtaaa caggcagagt atgtcaactc attaaatgta ttcccagttc
     5821 cagatggtga tactggtacg aatatgggga tgactatgga aaacggagcc aaggaagttt
     5881 cggacaaacc agcgacgact gttggagaag ttggtcaaat tctttctaaa ggtgtattga
     5941 tgggagcacg tggaaattca ggtgtaatta catcacaatt attccgtggt tttggtcaaa
     6001 gtatcaagga taaagaagaa ttaacgggtc aagatttggc acacgctttc cagaatggtg
     6061 ttgaagtagc ttataaagcc gttatgaaac cagttgaagg aactatcttg acagtatcac
     6121 gcggtgcagc taccgctgct ctaaaaaaag ctgaagaaac tgatgatgca gttgaagtta
     6181 tgcgcgcaac tctcaaaggt gctaagcgtg ctcttgctaa aacaccggat atgctacctg
     6241 ttcttaaaga ggttggtgtt gttgattctg gtggtcaagg actcgtcttt atttacgaag
     6301 gtttcctctc agcactgact ggggaatata tcgcatcaga agattttaaa gcaactcccg
     6361 caacaatgac tgaaatggtt aatgcagagc accataaagc tgtagtaggc cacgtagcca
     6421 cagaagatat taaatatggc tattgtacag aagtcatggt tggtttaaag caaggaccaa
     6481 cttacgttaa ggaatttaac tacgaagaat ttcaaggtta tcttagcaat ctaggggatt
     6541 ctttactagt tgttaatgat gatgaaattg tcaaggttca tgttcataca gaagatccag
     6601 gtcttgttat gcaagaaggt ttgaaatatg gatcgcttgt gaaggttaaa gttgaaaaca
     6661 tgcgtaacca acatgatgcc caaatgcaaa aggttgaagt tgaagaaact gtcaaagaaa
     6721 ctaaagaata tggtattatt gctgttgtag ctggtgacgg tctagctgag atttttaaat
     6781 cacaaggtgt tgattatatc atctctggcg gccagactat gaacccatct acagaagata
     6841 ttgtaaaagc gattgaaaaa gtaaacgcta gaaatgttat tattttacca aacaataaga
     6901 acatcttcat ggctgcacag tcagctgcag atgtcgttga tattccagca gcagtagtag
     6961 agacacgtac agttccacaa ggatttacta gcttacttgc ttttgatcct gctaagtctc
     7021 ttgaaactaa tgtagctgat atgacaaata gcctctcaga tgtcattagt ggtagtgtga
     7081 ctttagctgt tcgtgataca acgatagatg gccttgaaat tcatgaaaac gacatccttg
     7141 ggatggttga tggtaaaatt ctagtatcta caccagatat ggaaaaagca cttaaagaca
     7201 cttttgataa aatgattgat gaagatagtg aaatcgtgac aatctatgtt ggtgaagatg
     7261 gtaaacaagc attagccgag accttgtcgg aatatcttga ggagacttat gaagatgttg
     7321 aagttgagat ccaccaagga gatcaacctg tatatccata cctcatgagc gttgaataat
     7381 tgataaaata aaaaattagg agagatactt ctaatttttt attttatcag agtttctatt
     7441 ttagtatgat aaagcatcag tattttgaaa attttgacaa attgtgctag aattgtttta
     7501 aaacattcat acaaaggaga tttatgatgt cattaattat tcttacggtt attttggttt
     7561 tagtgattgt tctgttgata acttctttat acgttgttaa acaacagacg gtagccatca
     7621 ttgaacgctt tggtaaatat caaaagactg ctactagcgg tattcatatt cgtgtaccat
     7681 tgggtatcga taagattgca gcacgagtgc agttacgtct tttacagagt gaaattatag
     7741 ttgagacaaa aacaaaagat aatgtttttg tcactttaaa tattgctaca caatatcgtg
     7801 tcaatgaaaa taacgttaca gacgcttatt acaaattaat aaagccagaa gctcagatta
     7861 aatcttatat cgaagatgct ttgcgttcat cagttcctaa acttacatta gatgagctat
     7921 tcgaaaagaa agatgaaata gccttggaag ttcaacatca agtggcagaa gaaatgtcaa
     7981 catacggcta catcattgtt aagactttaa ttactaaggt tgagccagat gcagaagtaa
     8041 aacaatccat gaacgaaatc aatgctgcac aacgtaaacg tgtagcagct caagaattag
     8101 cgaatgctga taagatcaaa attgtaactg ctgcagaagc agaagcagaa aaagatcgtc
     8161 tacatggtgt gggtattgcg caacaacgta aagcaattgt agatgggtta gctgattcga
     8221 ttcaagaatt gaaagatgct aacgtgacat tgacagaaga acaaatcatg tctatcttgt
     8281 taacaaatca atatttagat actttaaata catttgctat taatggtaat caaacgattt
     8341 tcttaccaaa taacccagaa ggtgttgaag atattcgtac acaagtttta tctgctctta
     8401 aaacaagata atgaactttt tatagaagca aaataaccag agcatttgct ctggttattt
     8461 tgtgtctgac ttatttatta taattggaga gacggaaaaa agttattttt taccgtaacg
     8521 tttgtactca tgaatgattt tcagagcacg ttttctagtt ttaatgttag gacttttaag
     8581 tctgcgatag gctgacgata ggtctgtttt ttcagccatg attacctcct atagacaatt
     8641 tattaacgtt aacagacacc agtataccac aaaaaaatta actggtaaag tactttgttg
     8701 ataaatttaa aaataaatag caaagtaatt aaatatcaat caaaagtaat aagttcaaag
     8761 atatattaaa cctttattaa tgaggtttag atttgacaag tgatgttaag tgaaagatga
     8821 atgaggaaaa tacccaacga gaaagtagct aatggctatc actgtgcata taaaaaaacg
     8881 atcgatgagt gatcgttttt cagcaaacta aataattatt tttaatgtaa ttgaatgaaa
     8941 atttatactt gtgaaagtgt atcggttaag ttaataaggt cagtttttat ttatcgtaag
     9001 cacttaggaa gtgttttgga gcgtatccac gagagtagat aaacccggca tcgcgtgaag
     9061 gatagcttga ttcagaaggc gcatacccac gagagtagat aaatccggca tcacgtgaag
     9121 gataagacac tgtgtctgcc ttcgcaagac ttgctgaagt agctaataat agagtgacag
     9181 ctgaagctgc tgttaaaaca ctgattttaa gtttcattat aaaatccttt caaagataat
     9241 aaactattct tgcacagtgg ttagttcatt ttgtgcatac tatattatat catcaaaagc
     9301 tcattaaaac aacatgtttt taattttaaa caaatatata attaattaaa tatcgattaa
     9361 aattaaaaaa tgagatttag taacttattt aatagtatac aatccaataa cgactgtcta
     9421 atttgatcta tagttgaggc tagtcgtaga tttcatcaaa gatgtggtaa taaaaaatcc
     9481 cctccagttc cattgtaatg atgacaatga caaaacctgt aggggtataa atagtctata
     9541 tttaaatgtg gataaatttt attttaagaa gcgttgtaga aattccttag ttctttcttg
     9601 tgttggattt tcgaaaagtt gcttaggagt accctgctct gcaatgattc ctttgtccat
     9661 gaaaataacg cggtctgata cttctttagc aaattccatc tcgtgtgtca caattatcat
     9721 tgttaatcca gatttagcta gatcttgcat tgttttaagg acctcaccaa ccatttctgg
     9781 atccaaggct gaagttggtt catcaaaaag tatagcttct gggttaacag aaagtgctcg
     9841 ggcgattgcg acacgttgct tttgcccacc tgataattgt tttggtttag ctttccaata
     9901 ttgctcagtc atgccaacag catttaagtt ttctttagca atcttttcag cttcttggcg
     9961 ttcacgtttt agaacagtag tctgtgcaac aatagcgttc tctaagatgt ttaaattctc
    10021 aaagaggtta aaagattgaa agaccatccc aagtttttca cgataattat ttaaatcata
    10081 gcctttttca agaacattgt gaccgtgata taagatttct cctccacttg gttcttctaa
    10141 gaggttaata gaacgtagga aagttgattt tcctgatcca gaagaaccaa tgatagaaat
    10201 aacttcgcct ttgttgaccg aaagcgaaat gtcttttaac acttcattgc ttccgtaaga
    10261 ttttttcaaa tgtttaattt ctaaaatagc ttgtgtcata ccaacctcct agacttgtaa
    10321 ttgattcgca ccggttgtgt aattatctga atcaaaacgt ttttcaatgt aacgtaagat
    10381 acgtgttacg gtgaaggtta agatgaagta aataatcgca ataatggtga atgtttggaa
    10441 atattggtag gtttgtgttg caacagtatt accagagaag tagagttcga caacagagat
    10501 aacgttcagt acagaggtat ctttgatatt gataacaaac tcattaccag tagctgggag
    10561 tatgttacgt acaacttgtg gtagaacaat ttttcgcatg gtttgtccat gggtgaaacc
    10621 aagtgcagtt gcggcttcaa attgtccttt atcaacagag aaaataccac cacgtacaat
    10681 ttcgctcata taagcaccag tattgattga aacaataaat atagctgcta gtgtacggtc
    10741 cagcgaaact ccgaaagctt gtgctgttcc gtagtagata accattgatt gtacaatcat
    10801 aggtgtcccg cggaagacct caatatagat gttaagtaac caacctaata atttttgtaa
    10861 ggcagccttt aacttattgt cagattttgg tgctgtacgg aaaacaccga tgagaaggcc
    10921 gataaataat ccgacgatag ttcctaccat agaaattagg agggttgtag ctgttcctct
    10981 gaggaattga gaaccattgt tttttaaaat agttgccatc tgttcaaaga agtttggttt
    11041 tccatttttt tcttttttaa cggatggttg ttctttaatc atcttatcca tcaaggcgat
    11101 ttgtttatct ctagaaatgc tttcgagaac ttgattgact tgattaatac gattgtcacc
    11161 tttacgcatc ccaacggaaa tatttgtatc ggcatcagat gttttaaacc cttggtgcag
    11221 ttcaatcatt tttaatttag gattagcagt ttgagcggaa gttgcatctg gacgttctga
    11281 aacataggcg tctacaacac cagattctac agcttgtctt aattgattaa agtcccccat
    11341 ggcaacttct tttttgacac cattgatttg atcaatgagg ttgtataagt agactccttg
    11401 ttgggcagtt actttagcat ttttgaaatc actaatattt ttagcattag tatattttcc
    11461 ttcagcgtta acaactagag taggttctga aatgtagtaa ggctttgaaa aattaatttc
    11521 ttttttacgt tcttcggtag gactcattcc tgcaataatc atatcgagtt ttccagaagt
    11581 tagtgctgga acaaggcctt cccattttgt tttgacaaca acaacttttt tatttaactt
    11641 tttagctaac tttttagcga cttgtacatc ataaccatta gcatattgat cagttccttc
    11701 aataggaact gcaccatttg tgttatcatt ttgtgtccag ttgaaaggcg cataggctgc
    11761 ctccataccg acacgtagat attcatctgc ttgtatactt gtcatacctc caaaggtgat
    11821 aagcaaagca gtgaataaac ttagtaatat ttttttcatt caacagcctc cgataagtta
    11881 cttcctccta ttttaccgaa aaacaaggtc gatttcaata tcattatctt attgaaagcg
    11941 attaattgta tttttatgtt aaaatggtat acaaacaaag gagggaggat gataatgaag
    12001 aaatgttttt tggctatttg tttagctctt agttttttta tggtttcagt tcaagcagat
    12061 gaggtggact ataacattcc tcattatgag ggtaatctaa ctattcacaa tgataatagt
    12121 gctgatttta cagagaaggt tacttaccaa tttgattcgt cctataatgg acagtatgtc
    12181 acgttaggta cggcgggtaa gttacctgac aattttgata ttaataataa gccacaggtt
    12241 gaagtttcaa ttaatggtaa agtaaggaaa gttagttacc agatagaaga tttggaggat
    12301 ggctaccgtt tgaaagtgtt taatggtggt gaagcaggtg atactgttaa agtcaatgtt
    12361 cagtggaaac taaaaaatgt tctatttatg cataaggatg ttggtgaact taactggatt
    12421 cctattagcg actgggataa aacgttagag aaagtagatt tttggatatc aactgacaaa
    12481 aaggttgctc tttctcgtct ttgggggcac ttgggttatc ttaaaactcc tcctaaaata
    12541 agacaaaata ataatcgtta ccatttgaca gcttttaatg taaacaaacg attagaattt
    12601 catggttatt gggatagatc ttattttaat ctacctacaa acagtaaaaa taattacaag
    12661 aaaaaaattg aacatcaaga gaagataata gagcgtcatg gttttatcct aagtttcttg
    12721 ttaaggatat tattaccttc attctttatt attgtgacac tattcatctc aattagggtg
    12781 ttcctgttta gaaaaaaagt taataaatac gggcaattcc ctaaggatca tcatttatat
    12841 gaagcacctg aggacctttc accattagag ttaactcaaa gcatttatag tatgagcttt
    12901 aaaaattttc aagatgagga gaagaaaact caccttatca gtcaagaaca actcatacag
    12961 tcaattctat tagacttgat tgatagaaaa gtattgaatt atgatgataa cttgttatct
    13021 ctagctaact tagatagagc ttctgatgca gaaatagatt ttatagagtt tgcttttgcg
    13081 gattctacga gtttgaagcc agatcaactc ttttctaatt accaatttag ttataaagaa
    13141 acactacgtg aactgaaaaa gcagcacaag gcttcagatc tgcaaaatca aatgagacgc
    13201 cgaggaagta atgccttatc aagaattacg cgtctcacaa ggttgatttc taaagacaat
    13261 ataaactctc ttagaagaaa gggaatttca tccccttatc gtaaaatgtc ttcagaagag
    13321 tctaaagaat tatctaggtt aaaaagattc agttacctat cacctcttat ttcttttgtt
    13381 gttataattt atacgctttt tttaaattat tttacctatt tctgtatcta tctcttattg
    13441 tttggtgtta tcctgttgtt gaataaaatc atttttatga tgacaagaaa aataagtaac
    13501 ggttatattg taactgaaga tggagcaagt cgtgtctacc aatggactag ttttaggaac
    13561 atgctaaggg atatcaaatc gtttgatcgt tcagagttag aaagtatcgt attatggaat
    13621 cgaatattgg tttacgctac tttattcggc tacgctgacc gtgttgagaa agtactcaga
    13681 gtgaaccaaa tagatattcc agaaagattt gcaaacattg atagtcatcg atttgcgatt
    13741 tcagtcaatc aatctagtaa tcatttttca acgataactg aagatgttag tcacgcttct
    13801 aattttagtg ttaattcagg cggttcttca ggtggtttct caggcggcgg aggcggcgga
    13861 ggtggcggtg ccttctaatt ttccaaaagt atggtataat aaagaaaaat ttatggagtt
    13921 attatgttaa taattgaatt actcaaagcc ctatttttag gagtcgttga gggagtgaca
    13981 gaatggttac ctgtttctag tactggccat ttaatcttgg ttcaagagtt tatgaagttg
    14041 aaccaaagca aatcctttgt tgaaatgttt aatattgtta tacaacttgg ggcaattatg
    14101 gcagttattg tcatttattt taaacgcctt aacccatttc agccaggtaa atctgctaga
    14161 gaaattcgtt taacttggca attatggtta aaagtagtga tagcttgtat tccatctatt
    14221 ttaattgctt taccatttga taattggttt gaagctcatt ttaatttcat gattccgatt
    14281 gcaatagccc taatctttta tggttttgtc ttcatatggg ttgaaaaacg taatgcacac
    14341 ctcaaaccac aggtaaccga attggcaagt atgtcttaca agacagcttt cttgattgga
    14401 tgtttccagg ttctcagtat tgttccggga accagtcgtt ctggagctac tattttagga
    14461 gcaattatta ttggaactag tcgttcggtc gctgctgact ttactttctt ccttgccatc
    14521 ccaactatgt ttggttatag tggacttaag gcggttaaat attttttaga tggtaacgtc
    14581 ttgagtttag accaatcttt aatactttta gtagcaagtc tgacagcttt cgtagttagt
    14641 ttatatgtta ttcgtttctt gacagactat gtcaaacgac acgatttcac catctttggt
    14701 aagtatcgta tagtcttagg aagtttactc atcctctact ggttagttgt tcatttattc
    14761 taaaatataa cataaaaagc tgagtattcc tcagcttttt agcgtgttag gctgtgaaaa
    14821 attgctaaaa aatagctttt tcggtataat aagaaaactg aatactcgta tgaggtgatg
    14881 atatggaaat gaaacaaatc agtgaaacaa cactgaaaat tacaattagt atggaagatt
    14941 tagaagatcg tggtatggag ctgaaagatt tcctaatccc tcaggagaag actgaggaat
    15001 ttttctattc tgtcatggat gaattagact tgccagaaaa ctttaaaaat agtggtatgt
    15061 taagttttcg agtaacacct aaaaaagatc gcattgatgt ttttgttaca aagtctgaat
    15121 taagtaaaga tttaaattta gaagaattag cagatttggg tgacatttca aaaatgtctc
    15181 cagaagactt ttttaaaacc ttggaacaat cgatgttgga aaaaggggat acggatgccc
    15241 atgccaaatt agcagaaatt gaaaatatga tggataaagc aactcaagaa gtagttgagg
    15301 aaaatgtttc tgaagaacaa cctgaaaagg aagtagaaac gattggatat gttcactatg
    15361 tctttgattt tgataatatt gaagctgtag ttcgattttc acaaacgatt gattttccaa
    15421 tagaagcttc tgaactctat aaaaatggta aaggctatca tatgacaatt ttgctagact
    15481 tggaaaacca accaagctac tttgctaatt tgatgtatgc tcgaatgctt gaacatgcta
    15541 atgtgggcac aaaaacacga gcttacttga aagagcatag tattcaattg attcatgatg
    15601 atgctatttc aaaattacaa atgattgaga tgggataaaa tgataccttt tacaattgag
    15661 tatatttttg tattgattgg agcattttta ttatcaatca ttctaactcc tattattcga
    15721 gttatatctt tgaaggtagg agcggttgat aaaccgaacg ctcgaagaat caacaaagta
    15781 ccaatgccaa gtagtggtgg tttagcaata tttctctcct ttgttgtaac aacattattt
    15841 ttcatgccaa tggcggccag tagacacttt atagaagtct cttattttca ctacattttg
    15901 cctgtaatta ttggcggtct cgtagttaca acaacaggat ttatagatga catctttgaa
    15961 ttaagacctc gttataaaat gttgggaatt attattgcag ctattatcat atggaaattt
    16021 acacatttta gatttgatag ttttaagatt ccaataggtg gtccactctt ggagtttggt
    16081 cctattttaa ctttcttttt gacagtttta tggattattt caatcactaa tgctatcaat
    16141 ctgattgatg gtctagatgg tctagttagt ggtgtttcaa tcatcagtct agcaacgatg
    16201 gcagtggttt cttatttctt tttacctaaa atagattttt ttctcacgct aactattgtt
    16261 attttaatcg cctcaatagt tggttttttc ccttataatt atcatccagc cattatctac
    16321 ctaggtgatg ctggtgcctt gtttatagga tttatgattg gtgtattatc gcttcaaggt
    16381 ttaaaaaact ccacagctgt tgcagtgata acacctgtca ttattttagg agttcctatt
    16441 ttggatacag ctgttgctat tgtgaggcgt aaattatcag ggaaaaagat ttctgaagct
    16501 gataagatgc acctgcatca ccgattgcta tctatgggat ttacacatag aggagcagtt
    16561 ttagttgttt atggtattgc aattatcttt tcgcttatcg ctttgctttt aaatgtttct
    16621 agtcgtattg ggggaatttt tcttctattg gctctactat tagtaatgga aatttttata
    16681 gaaggtttaa atatttgggg tgagaatcgg acaccgctat ttaatctctt aaagttcatt
    16741 ggcaacagtg attacagaca aagtgttatt gctaaatata gcgataagca tcaaaaataa
    16801 aagcctgaaa aggctttttt agttttaatc atttagatag cagtcacacc tcatttttgc
    16861 tataattatt tagaatataa tatcgggtga gtgccctata atatcttctt taaaccatag
    16921 agaaatatca catattctct gttaaccaag aaaaggagtg tacaatgtct gtacttgaaa
    16981 tcaaaaatct tcatgtttct attgaagata aagaaatttt aaaaggtctt aatttaacgc
    17041 tgaaaacagg agaaattgct gctatcatgg gtccaaatgg tactgggaag tcgacacttt
    17101 cagcagctat tatgggaaat cctaattatg aagttacagc aggtgaaatt ctttttgatg
    17161 gtgaagatat tttagagcta gaagtggatg aacgtgctcg tttgggtcta tttcttgcga
    17221 tgcaataccc ttcagaggtt ccagggatta ctaatgctga atttatccga gcagctatga
    17281 atgcgggaaa ggctgatgat gataaaattt ccatccgcca atttattact aagttagatg
    17341 aaaagatgga actacttggc atgaaagaag aaatggcgga acgttatctt aacgaaggtt
    17401 tttcaggtgg tgagaagaag cgcaatgaaa ttttacaact cctaatgtta gagcctaagt
    17461 ttgccctctt ggatgagatt gactctggcc tggatattga tgctcttaaa gtggtatcaa
    17521 agggagtcaa tgaaatgcgt ggtgaaggtt ttggtgctat gattattaca cactatcaac
    17581 gccttttgaa ttacattaca ccagataaag tacacgttat gatggacggg aaggtagtat
    17641 tgtcaggagg accagaatta gcggttcgcc ttgagaaaga aggttatgct caaatcgctg
    17701 aagagcttgg tttagagtat aaagaagaag tctaatttgt cacttcaact agaagagaaa
    17761 aggagttaca aatgtctaaa gaagctatat taaatttttt acaagccaaa ggtgagccta
    17821 cttggttaca agaattacga ctaaaggctt ttgaaaaaat agaagaacta gaattaccag
    17881 taattgaacg tgtaaaattt catagatgga atttaggtga tggaactatt ttagagaatg
    17941 actatacagc taacattcca gatttcacag agttaggaaa caatcccaag ttggttcaaa
    18001 ttgggaccca aactgtcctt gaacaagttc ctatggaatt aatagagaaa ggcgttgtct
    18061 ttacagattt ctattcggct ttggaagaaa ttccagaagt catagagcgc tactttggga
    18121 aagcacgtcc ttttgaagaa gatagacttg ctgcctatca cacagcttac ttcaacagtg
    18181 gtgcagtgct ttatattcca gataatgttg aaattactca accaatcgaa ggtctttttt
    18241 atcaagatag tcaatctaaa gtaccattta ataagcatat ccttcttatt gttggaaaaa
    18301 atgcaaaagt tagttactta gagcgatttg agtcaattgg tgatgggaca gaacgaacaa
    18361 gtgctaatat ttctgtagaa gtaattgccc aagcaggtag ccaaattaaa tttgcctcaa
    18421 ttgatcgcct cggagaaaat gtgacaacgt ttataagtcg tcgtggtcgt cacagtagcg
    18481 atgcaacaat agattgggcc cttggggtga tgaatgaggg caatgttgtt gctgactttg
    18541 attctgattt aattggtgat ggaagccacg ctaatttaaa agtagttgct gcttcgtcag
    18601 ggcgtcaagt tcaaggaatt gatacgcgtg ttacaaacta cggctgtaat tcagtaggac
    18661 atattttaca gcatggggtt attttagagc gtgggactct tacttttaac ggtatcggac
    18721 atattataaa aggtgcaaag ggagcagacg cccaacaaga aagccgagtt ctcatgttat
    18781 cagacaaagc acgttcagat gctaatccta tccttttgat tgatgaaaat gatgttacag
    18841 ctggtcacgc agcctcaatt ggtcaggttg acccagagga tttatattat ttgatgagcc
    18901 gtggcttgaa ccaaaaaaca gcagagcaat tagttattcg tggattttta ggaacagtca
    18961 ttgctgaaat accagtaaaa gaagttcgcg atgaaatgat tgccgttatt gatacaaaac
    19021 tagaaaaacg ttaatatgac attactagat agttataaac taaagcaaga tttcccaatt
    19081 ttaaatcaat tagttaatga tgagccgctt atctatttag ataatgcagc aacgacacaa
    19141 aaaccgaatc aagttttaga aactttgcga gattactacc aaaatgacaa tgcaaatgtc
    19201 catcgtggtg ttcatacttt agcagaacga gcgactgctc agtacgagac tgctcgtgaa
    19261 aaagcaaggc agtttttaaa tgctaaatca agtaaagaaa ttttattcac acgtggcacc
    19321 acaacaggat taaattgggt agctaagttt gccgaaagta ttttagaacg aggtgatgag
    19381 gtacttattt ccattatgga gcatcattct aatattatcc catggcaaca agcttgcgag
    19441 agaactggtg ctaaattggt ttatgcctat ctaaaagatg gttctttaga tttagaagat
    19501 ttctataata aattatcctc taaaactaaa tttgttagtt tggcacatat ctcaaatgta
    19561 cttggctgta ttacaccagt aaaagcaata gcagaacgtg tgcatcaagt tggggcatac
    19621 atggttgttg atggagctca atcagcgcca catatggcga ttgatgttca agaccttgat
    19681 tgtgatttct tcgccttatc tggccataag atgcttggtc ctactggtat tggtgttctt
    19741 tatgggaaag aaagcatttt agataagatg ccacctgtcg aatttggtgg tgaaatgatt
    19801 gattttgtct atgagcatag tgcaacatgg aaggaattgc cctggaagtt tgaagctgga
    19861 acgcctaata ttgctggagc gatcgctttt ggagaagctt tagattattt aacggatgtt
    19921 ggtatggatg agattcatca atatgaacaa agtttagttt cttatgtgtt gcctaaatta
    19981 caggctatcg atggtttaac tatttacgga cctagtgatg ctgaatctca tgtaggcgtt
    20041 attgccttca atttagaagg tttacaccca catgatgtgg caactgcaat ggattacgaa
    20101 ggagtggctg ttcgtgcagg tcatcattgt gcacaacccc ttattaatca tcttggtatc
    20161 cattcggcgg ttcgagcaag tttctatttc tataatacca aagaggattg cgacaaattg
    20221 gtagacgcta tccaaaaaac taaggagttt ttcaatggca ctctctaaat tagataacct
    20281 ctacatggcg gtggtagctg atcattcaaa acaccctcat catcatggat ttttagaagg
    20341 cgttgaacaa gtgcagttaa acaatcctac atgtggagat gttatttcct tatctgttaa
    20401 atttgatggg aatattattt cagatattgc atttgctggt aatggttgta caatttctac
    20461 ggcttcatca agcatgatga cagatgcagt tattgggaaa acaaaagaag aagcattgca
    20521 gcttgctgat gtattctcca aaatggttca aggcaatcaa aatccaaaac aagagaaact
    20581 tggggatgca gagtttctag ctggggtatc aaaatttcca caacgtatca aatgtgctac
    20641 gctttcgtgg aatgctttaa gaaaggctat tgagagagat aatcaggcag aactctgaca
    20701 accatagttg aaggaattac atttaaacaa cttttactga cataaggtag tctagtagaa
    20761 gaatgtctta aatttaaata ttaaagaaag gataagtatg tccgaaataa atgaaaaagt
    20821 agaaccacag ccaatagatc ttggggaata taaatttgga tttcatgacg atgtaaagcc
    20881 aatttattct acgggaaaag gtctaaatga ggctgttatt cgtgagttat ctgcagctaa
    20941 gggtgaacct gagtggatgt tggactttcg tctaaaatcc ttggaaacgt ttaataaaat
    21001 gccgatgcag acctggggag cagatttatc agatattgat tttgatgata ttatttatta
    21061 tcaaaaagca tctgataaac ctgcgcgtga ttgggatgat gttccagaaa aaatcaaaga
    21121 aacttttgaa agaattggga ttccagaagc tgaaagagcc tatcttgcag gagcatcagc
    21181 acaatatgaa tcagaagtag tttatcacaa tatgaaagaa gaatatgata agctgggtat
    21241 tgtttttacg gatactgact ctgcacttaa agagtaccca gagctattca aaaaatattt
    21301 tgctaaactt gtccctccaa cagataataa attagctgct ctgaactctg ctgtatggtc
    21361 aggtggaaca tttatttatg ttcctaaagg tgttaaggtg gatattccac ttcaaactta
    21421 cttccgtatt aataatgaaa atactggaca atttgaacgt actctcatta ttgttgatga
    21481 gggagcaagt gttcactatg ttgaaggttg taccgcccca acttattctt caaatagttt
    21541 acatgcagct atagttgaaa tttttgcact tgatggagct tatatgcgct atacgactat
    21601 tcaaaattgg tccgataatg tctataattt agtgacaaaa cgtgctaccg ctaaaaaaga
    21661 tgcaacagtt gagtggatag atggaaatct aggagctaaa acaacaatga aatacccatc
    21721 ggtttacctt gatggtgaag gagcacgtgg cacgatgttg tctattgctt ttgcaaacaa
    21781 aggacaacac caagatacgg gtgcaaagat gattcataat gccccccata ctagttcatc
    21841 cattgtctct aaatcaattg ctaagggtgg gggaaaagtt gattatcgag gtcaagtgac
    21901 atttaataaa gattccaaaa aatcagtgtc acatatagaa tgtgacacca tattgatgga
    21961 tgatatttca aaatcagata ccataccgtt taatgagatt cataattcac aggttgcttt
    22021 agagcatgaa gcaaaggtgt ctaagatttc tgaagagcaa ctgtactact tgatgagtcg
    22081 aggtttatct gaagctgaag caacagaaat gattgttatg gggtttgttg agccctttac
    22141 gaaagaatta ccaatggaat atgcggtaga gttaaatcgt ttaatttcct atgaaatgga
    22201 aggttcagtt ggttaattga agatttcaag agataataaa aagcccctat gttattgatg
    22261 aaggggcttt tatattagca cagttattat ctagagcgct ttataagtaa tactatccca
    22321 gctaaaagac aaaggctagc gatggtgccc agaacaatta aaatagatac aaaatgtgat
    22381 tggataaatt gatctgtttt tgtccaccac tgttcttttg tttgaggctg ttcttgaata
    22441 gatttagttt tggttttagt aacttctttt ttaggtttaa cggcttttaa tacaggagtt
    22501 ttagaagaaa tatttttagc gtctttaaaa cctttttcta caagagcgtt gacaaacgga
    22561 tgacgatagt actctccgtc ttggtctgac caatcgccaa ctcctaaaac cacagttatc
    22621 aagcgagtat tctggcgttt agctgtaact aaggcattaa aagcagcgct agggctagaa
    22681 ccagttttta agccatctac tccttctaat cccaatttag cgccgggggt agagtagtta
    22741 taagttgtaa atgtttcttc ataaggagtt ccgaccatgg ccttgacttc aggatatttt
    22801 gtatagttca gtatatcagg gtatttttta aggaaatgat aggttaaaat tgatagatca
    22861 cgtgcagtcg taacgttagt agcattgtta tcgtattctt ttggggagta aagtccatta
    22921 aaagcactcg ctaccgcccc actggggtta taaaagtgag tttttgtcat accgagtttc
    22981 ttggcggttt cattgattcg tttaataaag gcgtcaggat tgttttgtga taagtggtta
    23041 gcaatcataa tagttgctac attagatgac gggacagccg tcatagtaat cagttcacga
    23101 ataggataag caaccccagc atgaatattg ttattactaa tttcataaat cttacttatg
    23161 gcttggtctg tttctgttgc agtcactgtg gtgttgaggt ttgtttttcc tttagctaag
    23221 tcttcaaata gtaaatataa agtaaacatt ttagacatgc tagcgggatc acgttctaaa
    23281 tcggcgttat cttcccacaa aatatgacct ttattgtcaa taacgataga cgctttaggt
    23341 ttattaatat ctttaacatc atatccggct tctcgggtaa tttgcataat atctttatgt
    23401 tcttcagctt gcacagagtt tacacaagtc agggataata aaatgcagag gaaagatacg
    23461 attaatttag gcataggtaa ctcctgaaaa ctttttatta atattataac aaaaaatcac
    23521 taataaaaaa gacatttcat tagtgatttt taattttata tattctccta aacctagcta
    23581 aagaaactgc tgtgcagtaa ctccttgtgt caaagggaga ttactattaa agtttttcgt
    23641 taacgtagcg cacaaaatga ttccaccaca ctttgaggaa gaaactgcgc tcaatactat
    23701 cttttgccac taaatgaacg ctaggttcct ttatgagata gccttttcca accttattaa
    23761 ggtcaacgta ataagctttc cctaggttct ctttttttgt aataggagca gtaagctctt
    23821 ttttaaagtt aatttttaac tgattttgtt tagtgatatt tttcttctgt actactttca
    23881 aatcgttttt agcaacagcg atgaccgatt gttcttttcc gtctctcaca cttgcttctt
    23941 tacctttata tgcagctcct ttagctaata caaggttagg ttcgtaggtg tttgtgatat
    24001 agttcaagag agagttagtt gctgtaaagc gagcatattt gtctttatcg gccttatcag
    24061 catgcataat aacagtgagt agtctcattc cactttcagt agatgtagca ataaaagatt
    24121 ggccagctaa ttccgttgtc ccagttttca aacctgtaat acctggtcta aagacaggca
    24181 tatctggtag catgtagtta taagaatgca taatatcttt atcaaattta gcaacggact
    24241 tactagtaat cttaagaatg gaaggatatt cgttgaccaa atggtaggca gcaatagcaa
    24301 tatcacgtgc actcatttta ttttcgtcgt tttgtgacga ttttggataa atgtgattgc
    24361 ctaacatact attatttaag ccagaagcat tgactaggtg gctatcatga attccccact
    24421 tttccaattg agcagtcatt ttatcaacaa atttactttc agttcctgaa atatgttcag
    24481 ctaaagcaat ggctgcactg ttagcactag aaatcatggc agcgtccacg agttgtttaa
    24541 cagtatagcg ccttttttct aaaggaacat tactagcatc agattcgcgt gttagttgat
    24601 aagggtagtc agatatattt actttggtat tccacttgag gttaccgtta tcaatttctt
    24661 tatagaccat ataaacggtc attattttag tcaaggaagc aatagcagcg ggtttgttag
    24721 catctttttc atacaagatt tttcctgaat ctaaatcaac tgctaaggca tgtttagcgg
    24781 aagcattaaa agattcagca gagatggttg gcataaaaaa aatgcaacta agtaatagaa
    24841 tagaagtaat aatttttttc aaaataacac ctttagaaat tttttaaact ttgtccctcc
    24901 tattatacca tatcactatg tcatactagg aattattatt ttcagataat tgaatgaaga
    24961 aaacgtttgt tataaaaatg taaaaaaatg taaaaaaatg aaagttaaaa aattatctga
    25021 aaattttgcg tttttgtctt ttcttaagaa ttttttgtgg tataatgtta gtcactagat
    25081 aaatgaatta ggagaacaaa gctatggcaa aacagaaaaa taactggcgc cgtgttggag
    25141 ttggtgtcct tacacttgct tcagttgcga ctcttgctgc atgtggaagt aaatcagctt
    25201 cccaggattc taatggagcg attaattggg ctattccaac agaaatcaat acactagatt
    25261 tatctaaagt tacagacact tactcaaatc tagctattgg taactctagt agtaatttcc
    25321 ttcgcttaga taaagatgga aagacaagac cagacttggc tactaaagtt gatgtttcaa
    25381 aagatggctt aacttataca gctacattac gtaaaggctt gaagtggtca gatggcagta
    25441 aacttactgc aaaggatttt gtttattcat ggcaacgttt agttgatcct aaaacagctt
    25501 cacaatatgc ttaccttgct gttgaagggc atgtgcttaa tgccgataaa atcaacgaag
    25561 gacaagagaa agacttgaat aagctaggtg ttaaggcaga aggcgatgac aaagttgtta
    25621 ttactttatc tagtccgtct ccgcaattca tctactacct tgcattcact aacttcatgc
    25681 cacaaaaaca agaagttgtt gaaaaatatg gaaaagatta cgcaactact tcaaaaaata
    25741 cagtttactc aggaccatat actgttgaag gttggaatgg ttcgaatggt actttcacgc
    25801 tgaagaaaaa caaaaattat tgggacgcta aaaatgtaaa aacaaaagaa gttcgcatcc
    25861 agactgttaa aaaaccagat accgccgttc aaatgtataa acgtggtgag ttagatgcag
    25921 ctaatatctc aaatacttct gctatttatc aagctaataa aaataataaa gatgtcacag
    25981 atgttctaga agcgaccact gcctatatgg aatataatac tactggttct gtgaaagggc
    26041 ttgataatgt taagattcgt cgcgccttaa acttagcaac taaccgtaaa ggagttgttc
    26101 aagcagccgt tgatacaggc tcaaaaccgg caattgcttt tgcacctact ggtttagcca
    26161 aaacaccaga tggaactgat ttggcaaaat atgttgcccc aggttatgaa tataataaaa
    26221 ctgaagcagc aaaactcttt aaagaaggtt tggctgaatc aggcttgact aagctaaaat
    26281 taacaattac agcagatgct gatgctcctg ctgccaaaaa ctctgttgac tatatcaagt
    26341 ctacttggga agctgctctt ccaggactta ctgttgaaga aaaatttgta acctttaaac
    26401 aacgcttaga agacagtaga aaacaaaact ttgacattgt agtttctctt tggggtgggg
    26461 attatccaga aggatcaact ttctacggcc tctttaagtc ggattcacaa aataacgatg
    26521 gaaaatttgc taacaaggac tatgatgctg cttataacaa ggcaatttct gaagatgcga
    26581 tgaaaccagc agaatcagcg aaggactata aagaagcaga gaaaatttta tttgagcaag
    26641 gtgcttataa cccactctac ttccgtagtg gtaaaggttt acaaaatcca aaattaaaag
    26701 gtgttattcg taatactaca ggtttgtcaa tagactttac acatgcttat aaaaataatt
    26761 aaattacttt acactaaacc tttgcaagaa ttggtaattg ccagttcttg cttttggtta
    26821 agtaaagaaa gtgaaagagt ttgctatttt tagcaattct aagaaaagga aatgatatga
    26881 ttaaatatat tttaaagcgt gttgctattt tgttagtaac gctatgggtt gtcataaccc
    26941 tatcgttctt tctgatgcag atccttccag gaacacccta caataatcca aaactcactg
    27001 aagaaatgat cacattgctt aataaacagt atggtttaga caagcctgta tggcaacaat
    27061 atttgactta cctttggaat gtacttcatg gtgattttgg aacgagttat caatcagtga
    27121 atcagccagt atcacgtatg attagcttga gattaggagt ttcagtacat ctaggtgttc
    27181 aggctctcgt atttggtgtt ttaggcggta ttttggtagg agctatttcg gcacgtcata
    27241 agaatgataa agtcgatggt atcttaagtg ttattgcaac gctagggatt tctatgcctt
    27301 catttatcat tggtatctta ttattagact attttggatt taagtggaac ttgttgcctt
    27361 tatcaggttg ggggactttc tctcaaacaa ttttaccttc tttagctcta ggactcccta
    27421 cattagcatc tgtttcacgc ttcttccgta gtgaaatgat tgaaacttta aattctgatt
    27481 atgtgcaact agctcgttca aaaggtatga caattcgtca agtaactcga aaacatgctt
    27541 accgtaattc tatgattcca attttaactc taattggccc tttggcggcg ggtctgttaa
    27601 ctggttctgc actaattgag cagattttct cgattccagg aattgggcag cagtttgtta
    27661 cttcgattcc aacaaaagac tatcctgtta ttatggggac aaccattgtt tatgcagtaa
    27721 tgttgatggt tgctatcttg ataacagacg ttgttattag tatcgtagac ccacgtgttc
    27781 gcttgcagta aggagatgtt atggcagata aaaacagaac atttaaactt gtaggtgcag
    27841 gatcttctag cacacaagaa aaaattgaaa agcctgctct ttcgtttatg caagatgcgt
    27901 ggcgtcgctt gaaaaaaaac aaattagcag tagtttcact ctatttatta gctcttttac
    27961 ttactttttc gttagcctca aatttatttg taactcagaa ggatgctaat gggtttgatt
    28021 cgaaaaaagt aacgacatat cgcaacttac cacctaaatt gagttcaaac cttccttttt
    28081 ggaatggtag cattaagtac gctggaaata cagaatctac agacgcttat aagtctcaaa
    28141 atgtccctga aaaagttaag tatgctttag gtacagattc tctagggaga agtgttgcta
    28201 agcgcatcat cgtaggtatt cgaatttcct tacttgtagc tattgcagct acattcattg
    28261 atttaattat cggtgttaca tacggtcttg tttcaggttt tgctggagga cgccttgata
    28321 cattaatgca acgtgtcgta gaggtaattt catcaattcc aaacttagtt attgttacaa
    28381 tgttaggttt ggtccttggt aacggtatta cggctattat tatctctatt gcttttaccg
    28441 gatggacttc tatgtctcgt caggtaagga atttaaccct atcatatcga gaaagagagt
    28501 ttgtgcttgc tgcacgttca cttggggaaa gtccaattaa aatagccttc aaacatattt
    28561 tacctaacat ttctggtatt atcattgtac aaattatgat gacaatccca agtgctatta
    28621 tgtatgaagc ggtactttca gcaatcaatt taggtgttaa accaccgaca gcttcattag
    28681 gatcattgat ttcagatgca caagaaaatt tacaatatta tccatatcaa gttatcttgc
    28741 cagctttagc attggtaatg atttcattgg catttatttt gcttggtgat gggttgcgag
    28801 atgcttttga tccaaaatct agcgatgaat aaggaggtat aatcatggaa aaagaaacta
    28861 ttttaagtgt taataatctt catgttgact tccacacata tgctggagaa gtaaaagcaa
    28921 ttcgtgatgt caactttgaa ttaaaaaaag gtgagactct tgcaatcgtt ggtgaatctg
    28981 gttcaggaaa atctgtaact accagaactt taattggttt aaatgctaaa aattcagaga
    29041 tatcagggaa tgttcaattt aaggggcgta accttgttga actatcagaa gaagagtgga
    29101 ctaaggtacg cgggaatgag atttctatga ttttccaaga ccctatgact agtttggatc
    29161 caacgatgaa aattggtatg caaatagcgg agccaatgat gattcatcaa aaaatttcaa
    29221 aaaaggatgc tttaaaatta gctcttgagc taatgaaaga tgtaggtatc ccaaatgctg
    29281 aagagcatat taacgattac ccacatcagt ggtctggagg aatgcgtcaa cgtgcggtta
    29341 tagcgattgc tttagctgct gatcctgaaa ttcttattgc tgatgaacca acgactgctt
    29401 tagatgtaac aattcaagca caaattttaa acttaatgaa aaaaattcaa gcagaacgag
    29461 actcctccat tgttttcata actcatgact taggggtcgt tgcagggatg gcagaccgtg
    29521 tagcagttat gtatgcaggg aaaatcgttg aatttggaac tgttgatgaa gtcttttata
    29581 atccacaaca tccatatact tggggattat tgaattcaat gccgacaacc gaaacagaat
    29641 caggtagttt agagtcaatt ccgggaacac caccagattt gttgaaccca cctaaaggag
    29701 atgcatttgc agctcgtaat gaatttgcat tagatattga tcatgaagaa gaaccaccgt
    29761 acttcaaagt ttcagaaaca catttcgctg ctacatggct tttagatgaa agatcaccta
    29821 aggtgcttcc tccattaccg atccaaaaac gttgggaaaa atggaacgag attgaaggga
    29881 ggaaagccca atgactgaaa atcgaaaaaa attagttgaa gtcaaaaatg tttctttgac
    29941 cttcaataaa ggaaaagcta atgaagtgag agcaattgat aatgttagtt ttgacattta
    30001 tgaaggtgaa gtgtttggat tagttgggga atctggatca ggaaagacaa ccgttggacg
    30061 ttcaatttta aaactttatg atatttctga tggcgaaatt acctttaatg gagaagtgat
    30121 ctcacatctg aaaggtaaag cattacatag ttttcgtaaa gacgctcaga tgatttttca
    30181 ggatcctcag gctagcttaa acggccgtat gaagattcgt gatatcgtag cagaaggttt
    30241 agacatacat aaattagcta aatcaaaatc agatcgtgat agtaaagttc aggccctatt
    30301 agatcttgta gggttaaata aagatcattt aacacgttat ccgcatgaat tttcaggtgg
    30361 acaacgacaa cgtatcggga ttgcacgtgc tttagcagta gagcctaaat ttatcattgc
    30421 tgatgaacca atatctgcct tagatgtttc aatccaagca caagttgtta atttaatgca
    30481 aaaattgcaa agagaacaag gattgactta tttgtttatc gcacatgatt tgtctatggt
    30541 aaaatacatt tcagatcgta tcggtgttat gcattggggg aaactgttag aagttggaac
    30601 atctgatgat gtttataaca aaccaatcca cccttatacg aagagtctat tatcagctat
    30661 tccagaacca gatccggaga gtgaacgtca acgtgttcac cagccgtata atccagctat
    30721 tgagcaagac ggacaagaac gtcaaatgca cgagattaca cctggtcatt ttgttttatc
    30781 tacaccccaa gaagctgaag aatataaaaa acaaatatta taaaagatgg ctagcccatc
    30841 ttttattttt ttactaataa aataaattta ggtatggaat tttaaactct atgttattgg
    30901 tgttagccaa caggaaact
//
LOCUS       DATRSG010000017        30565 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRSG010000017 DATRSG010000000
VERSION     DATRSG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30565
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.017"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..682
                     /locus_tag="VBD89_000753"
     CDS             <1..682
                     /locus_tag="VBD89_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7135303.1"
                     /translation="IRRSIHSKMVNTFEKPVQLLESICEKGMHNLYFLDIEIKNDEQR
                     GLEVAKQIRQIDPYAQIVFVTTHSELMPLTFRYQVSALDYIDKGLSQEAFSRRIEDVL
                     LYANNACSKNLLEDSFYFKSRYSQVQVPFNDILYIETSLRSHRVVLYTAKDRMEFTAT
                     LGDILKQEPRLFQCHRSFLVNPLNIFKVDRSNKLIYLKNGSNCLISRNKIKDIMVVIE
                     ENQRGRGI"
     gene            679..2013
                     /locus_tag="VBD89_000754"
     CDS             679..2013
                     /locus_tag="VBD89_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO7135304.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKTLRSSEVSPIDKR
                     ILQFTNGCMVSYYLLMQFSTYLEYELAINTVIYRQFLLVVYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIDNHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(2084..2266)
                     /locus_tag="VBD89_000755"
     CDS             complement(2084..2266)
                     /locus_tag="VBD89_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135305.1"
                     /translation="MLTYKKALVRQVDTKDCGVTALASIAKFYGSKYLLNHLRELAKT
                     NRDGTTASGIIAGTTK"
     gene            2555..2728
                     /locus_tag="VBD89_000756"
     CDS             2555..2728
                     /locus_tag="VBD89_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7135306.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSENTAAANARNRM
                     GLYPGSVCRVYLV"
     gene            2797..3645
                     /locus_tag="VBD89_000757"
     CDS             2797..3645
                     /locus_tag="VBD89_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135307.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKPEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEQGNTAQLLKT
                     IEGKVWEITTEPDLSQIPNIAIVNEKVISDSLVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            3651..4805
                     /locus_tag="VBD89_000758"
     CDS             3651..4805
                     /locus_tag="VBD89_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO7135308.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRDFLIYYNQLKDDKTIDFNKINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNS
                     TYILIVLFTLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILA
                     YALILTVFADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGL
                     LFIASLNFFILSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTS
                     ALLLKLSRNGSHGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            5034..6068
                     /locus_tag="VBD89_000759"
     CDS             5034..6068
                     /locus_tag="VBD89_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7135309.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWFIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKGYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            6315..6740
                     /locus_tag="VBD89_000760"
     CDS             6315..6740
                     /locus_tag="VBD89_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO7135310.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKACSEDDFFD"
     gene            6758..7261
                     /locus_tag="VBD89_000761"
     CDS             6758..7261
                     /locus_tag="VBD89_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7135311.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVKQSEV"
     gene            7265..8074
                     /locus_tag="VBD89_000762"
     CDS             7265..8074
                     /locus_tag="VBD89_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7135312.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            8071..8898
                     /locus_tag="VBD89_000763"
     CDS             8071..8898
                     /locus_tag="VBD89_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7135313.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            9036..10685
                     /locus_tag="VBD89_000764"
     CDS             9036..10685
                     /locus_tag="VBD89_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7135314.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSGAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLR
                     SNHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            10689..11462
                     /locus_tag="VBD89_000765"
     CDS             10689..11462
                     /locus_tag="VBD89_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7135315.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            11474..12511
                     /locus_tag="VBD89_000766"
     CDS             11474..12511
                     /locus_tag="VBD89_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7135316.1"
                     /translation="MKEKQSKRLIYILLIVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKSQIL
                     KTYNRIRRNAD"
     gene            complement(12733..13230)
                     /locus_tag="VBD89_000767"
     CDS             complement(12733..13230)
                     /locus_tag="VBD89_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7135317.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(13230..14267)
                     /locus_tag="VBD89_000768"
     CDS             complement(13230..14267)
                     /locus_tag="VBD89_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO7135318.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNNTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            complement(14278..14733)
                     /gene="nrdI"
                     /locus_tag="VBD89_000769"
     CDS             complement(14278..14733)
                     /gene="nrdI"
                     /locus_tag="VBD89_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7135319.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            complement(14890..17292)
                     /locus_tag="VBD89_000770"
     CDS             complement(14890..17292)
                     /locus_tag="VBD89_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO7135320.1"
                     /translation="MSKHYFSKSIFALTVLTATTTSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNIAGTIYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGNLLKKK
                     QI"
     gene            17552..19768
                     /locus_tag="VBD89_000771"
     CDS             17552..19768
                     /locus_tag="VBD89_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO7135321.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            19778..20221
                     /locus_tag="VBD89_000772"
     CDS             19778..20221
                     /locus_tag="VBD89_000772"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO7135322.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            20476..21399
                     /locus_tag="VBD89_000773"
     CDS             20476..21399
                     /locus_tag="VBD89_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO7135323.1"
                     /translation="MKRIRKSLIFVLGIVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            21426..24008
                     /locus_tag="VBD89_000774"
     CDS             21426..24008
                     /locus_tag="VBD89_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7135324.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPINKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGNQVQVTKKDGSKVLVDIKTL
                     AEVK"
     gene            complement(24289..25065)
                     /locus_tag="VBD89_000775"
     CDS             complement(24289..25065)
                     /locus_tag="VBD89_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7135325.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNAAPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
     gene            25274..25747
                     /locus_tag="VBD89_000776"
     CDS             25274..25747
                     /locus_tag="VBD89_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7135326.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            25749..26054
                     /locus_tag="VBD89_000777"
     CDS             25749..26054
                     /locus_tag="VBD89_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7135327.1"
                     /translation="MIKILAACGAGINSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGSILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            26094..26581
                     /locus_tag="VBD89_000778"
                     /pseudo
     CDS             26094..26581
                     /locus_tag="VBD89_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
     gene            26822..29271
                     /locus_tag="VBD89_000779"
                     /pseudo
     CDS             26822..29271
                     /locus_tag="VBD89_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
     gene            29520..29944
                     /gene="lacA"
                     /locus_tag="VBD89_000780"
                     /pseudo
     CDS             29520..29944
                     /gene="lacA"
                     /locus_tag="VBD89_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
     gene            29965..30480
                     /gene="lacB"
                     /locus_tag="VBD89_000781"
     CDS             29965..30480
                     /gene="lacB"
                     /locus_tag="VBD89_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO7135328.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
BASE COUNT         9990 a         4827 c         5818 g         9930 t
ORIGIN      
        1 aattaggaga agtatacaca gtaaaatggt aaataccttt gaaaaaccag ttcaattatt
       61 ggaatcaatc tgtgaaaaag gtatgcataa tttatatttc ttagacattg aaataaaaaa
      121 tgatgagcag aggggcttag aggtcgcaaa acaaattaga caaattgatc cttatgctca
      181 gattgttttt gttacaactc attcggaatt aatgccttta acctttcgtt atcaggtatc
      241 agctttagac tatatcgata aaggactttc acaggaagca tttagcagaa ggattgaaga
      301 cgttttacta tatgctaata atgcttgcag taaaaactta ctagaagatt ctttttattt
      361 taaatcacgc tatagtcaag tacaagtacc ttttaacgat atcctttata tagaaacgtc
      421 attaaggtca catagagttg tcctttatac tgcaaaagac agaatggaat tcacagccac
      481 tttgggggat attttaaaac aagaacctag gctctttcaa tgtcatcgct cttttttagt
      541 aaatcccttg aatattttta aagtggatag gtcaaataaa ctaatctatt taaaaaatgg
      601 ttcgaattgt ttgatttctc gtaataaaat aaaagatatt atggtagtaa tagaggaaaa
      661 tcaaagaggt agaggaatat gaattctact ctatccatct tagagatagc tttaacgata
      721 atattaacaa tatttctctt ttctaaaatc agtggacgtc aattgacttt aatagagatt
      781 ggaatttgta ttgctctgag attaggactt gtaattttat ttatagcagc taatactatt
      841 attaatcttt catttctaaa gtactggctt ctccccatct atatgttaag tctggctttc
      901 ttgttgctaa gacctatatc taaaacttta gtagtctttt atggcttgtt tccaattgtt
      961 ctcagaaatc tttgttcacg ttctttagct ttttttgtct ttcctttgtt taatattgat
     1021 tttcatacaa ccaaccaggg gttttgggga tattttgttg atgtcttgtc tactctttgt
     1081 gttctcctat ttttaagatg gatgcaatac gactttaaaa ctttgcgaag tagtgaagtt
     1141 tcgcccatag ataagagaat tctccaattt acgaatggtt gtatggtcag ttattactta
     1201 ctgatgcagt tttcaactta tttggaatat gaattagcca tcaatactgt tatttaccgg
     1261 caatttttgc ttgttgttta ttggattata tttacgggag caatagccag tttagaccgg
     1321 agacttcgaa attctttaca ggagaggtta gctgttcaaa gagaactcca attggctaat
     1381 atgatagatt atagccacca tattgagggg ttgtataaag aagttaggag cttccgacat
     1441 gattatagta acatgttaac gaccttgaaa ctagggattg ataatgaaga tatatctatt
     1501 gtcaaatcag tttatcagtc tatctttaaa gaatctaata cacatttccg acatcctaag
     1561 tataatatag accggttggt taatgtagat aacccagcct taaaaagtct tttagcagcc
     1621 aagctttcac aagcgataga caatcatatt atggtatcta tagaagtacc agacagtatt
     1681 aaacctcaag ggatggaact gattgatttt attaccattg tatctgtttt atgggataac
     1741 gctattgagg catctttaga gacagacgat cctaagatag ccatgtctta ttttacatta
     1801 aaacataaac aaatttttat catagaaaat tcaactaagg aaaaggcaat agatacgtca
     1861 acgatattca attatgagat aagtagtaag gggcggaata gaggaatagg tctttacaat
     1921 gtcaggggag ttatcaatcg ttatccaaat atttctattg caacaagtag ccaagatttt
     1981 agtttctgcc aaactttcga aatcatatta tagttttaaa aagttcaatt tatctaagtt
     2041 tattattttc ttaataccat caatccaacc accaccagtg atattattta gttgttccgg
     2101 cgataattcc tgaagctgta gttccatctc gattagtttt ggctagttct cgtaagtggt
     2161 ttaataaata ttttgaacca tagaactttg cgattgaagc aagtgcagtt actccacaat
     2221 ccttagtatc aacctgacgc accaatgctt ttttgtacgt caacattgtg taattccttt
     2281 cttcgttatc gtattaatat tttgccataa aaatatatac ttattaaaaa tagcttaaac
     2341 agctattgca acgcgtccaa tggtttctat aaggatataa aggtagatag tacttttctg
     2401 ttttgaggcg attcaacgac atttcaggga ataaacgttt ctatttttat ttgaatgcta
     2461 tattaaatgt gtcaagttgc aacagacaaa aattttatat aggagatatt caaaataaat
     2521 gtaaaatcaa ttgaacaatt agaagttatg gataatgaag cattatcaca tgttgaaggt
     2581 ggccttggtc atagatgggt atacagtaag aaccggtaaa catacttata caagtgcatg
     2641 gcatctttct gaaaatactg ctgcagcaaa tgcaagaaat cgtatgggcc tttaccctgg
     2701 aagcgtttgt cgtgtctatc ttgtataatt gattttttat ttaagcgata gggaatcttc
     2761 cttgccgctt aagtttgtat agggagagac aatactatgt tattacagct agataatatc
     2821 actaaaagct acggaaaaaa aatagtactt aaccagattt cctaccagtt tactcctggt
     2881 ttgtatggac tcttaggagc aaatggtact ggcaaaacaa ccttattaaa tttgatgagc
     2941 cactttacat tcgcagatag tgggaatatt tattggaatg gacaggagca gtcggaagaa
     3001 ttttatcgtc acattggttt tttaccgcaa cattttcgct attatgatca atttactggc
     3061 attgctttct taaattatat cgctacctta aaaggtgttg ataaaaagaa agctaaacca
     3121 gaaattccta gattactaga gcttgttggt ttaggtgatg taggtaaaaa gaagatttca
     3181 tcatattccg gtggtatgaa gcaacgttta ggcattgctc aagcacttat caatgaccct
     3241 gaaatactta ttctggatga accaactgtt ggacttgatc ctaaggaacg ggttaaattt
     3301 cgccatattt tgagtcagtt atccaccaat aaaattatca ttttatcaac gcatattgtg
     3361 tcggatgtgg aagctgttgc caaagaaatt atagtattaa aaaatggtaa atttatcgaa
     3421 cagggcaaca ccgcacagtt gttgaaaact attgagggaa aagtgtggga aataacgact
     3481 gaaccagatt tatctcagat acccaatatt gctattgtta atgaaaaagt catttcagat
     3541 agtctggtat ttcgtgtagt gagtgatatt tgtccgagtg attcggcaca gttggttgtt
     3601 ccgactctag aagatttcta catctatcat tttagagggg agtaagtaag atgatatcaa
     3661 tttttccttt tgaattaaaa aaacttctac aacgtaaggc agcattgggg gcttttatta
     3721 tcttccttct tgctattttt ggtctatttt accagcattt ttttgttggg caaattagtg
     3781 gctattctgc agataaaata catggtcgag aagcggttgc aattaatcgt agaatagctg
     3841 aaaaatattc aggggattta tctgaagaac gagtaacaaa tattattaaa aactatgtta
     3901 atccgccagc aggacgcccc cctatatttg atgtttttag ttattatgtg gcagacactt
     3961 ttgatcgtga ttttttaatt tactataacc aattgaaaga tgataaaaca attgatttta
     4021 ataaaataaa tattaaagat caaaaacagt tgggttctgc gctaccacta aaacaactaa
     4081 agctaggtaa ctttgctcct tggaatcaac tttttgaagt aagtaatagt acttatattt
     4141 taattgtctt atttactcta ttcttatcag ctcctctttt ttcaggagat agggctaaga
     4201 agattaatcc tatactactg acgactcggt atggacgtgg tcagttgacc tgggctaagt
     4261 tagctagtac aatgaccatc ggtatttcca catttatcct agcatatgcc ctcattctga
     4321 cggtatttgc agattatttt ggttttagtg gttgggagac aagtgttcaa ctgaatttat
     4381 actggacatc acctttggct aatattatgg gatttcctgt acctatgaca atatcaactg
     4441 cttttgttca tattattgtc ttacaatttg ttggactcct ttttatagcc agtcttaatt
     4501 tctttatatt aagtatcaca aattcgccat taacatcttt tgctgtttca acgcttgcct
     4561 tttttgctcc tagcttttta atggctatgt ttaataaagg tgtgataaac aagttattaa
     4621 ctattttctc agtatcaacg acagacactt cagctttact tttaaaatta tcacgtaatg
     4681 gtagtcatgg ctttttcttt gatagttttt ctgctaatga aatctgtctg attagtctac
     4741 gtatgttaat tgcagtgtta ttgatagctt atacctatcg tttaatgcga cgaagaagtt
     4801 tatagttccc actggttatc tgaacaagaa attattctaa gctgattttc ttttgcgaaa
     4861 tcagcttttt tatttcctaa tatggttgat aaggtattaa caataattat cagataccat
     4921 ttaaggcgtt tcaatgactt ttggggataa ttgagatatt cttctgaaga gattggtatc
     4981 atagtaatga taacttatag aaagtaatgt taaggatgaa gagaagtcag tgcatggata
     5041 ataagcagca taatacatca atgattagct tgctacaata tcttttttcc atactggtca
     5101 ttttagttca tagtggaagg ttatttagtc aggatgtcat tcattttacc tttaaaagtt
     5161 tcctaggtcg gatggctgtt ccttattttt tgatttgtac tgctttcttt ttgagaggtc
     5221 gtatacagca aggtttgtgt aatcactcct actttcgtaa actgattaaa aagtatagca
     5281 tgtggactat catttatttg ccttatggtt atttcttttt tgaatctcta aatatagcga
     5341 agatttattt gctgccgggt tttattgtgg catttttgta tttaggtatg tcacatactc
     5401 tttggtacat ccctgctgtt attctaggtt ggttcattat tcaaggatta ttgaagtatg
     5461 taggaactag ggggacattt atcacagttg ttgtactcta ttgtattgga gcagtagaga
     5521 cctattctgt ctttgtacag tcaacaaaat tttatcccct gatgtccact tatatgtcaa
     5581 tttttcagac aacacgaaac ggattatttt acactccagt ttatcttcta gcaggatatt
     5641 tactctatga ttattttaat actgacctat ttaccaaatc gcgtggtctt aagtatattc
     5701 tcttcctttt acttctagct ttagagaatg ttcttattta ttttaatcaa gggttggata
     5761 aaaatttctt cttattagct cctttatgtg cagtattctt atttaactgg agtatacgta
     5821 catcactgtt taagggctat cgtttaagtc ctttaaaaca gttgagtgtc tattatttct
     5881 tccttcaccc tctttttgtt ggcatagtgt cttactgttt aaaatcaact tccttagttg
     5941 cacaccatca aggaaaagtg atttttgtgg tgaccttggc attaacacat gcttgtgctc
     6001 tactcataat aaaggttgtt aataagatac cctatcaaaa aattattcca ttatctcttt
     6061 cgaaatagtg attcatataa gagaaagttt aaagttatag tgatgtaaag gttgtgtaaa
     6121 ctattaggat acatgacctt tttattgttc tgtttttaaa tattaattat atagaatagt
     6181 ccacacatag agtagaatag tccattttta aaccgttttc atttgttaga atgatagtaa
     6241 aggttatctt attcaaagta ttgataaaac tttcagaact aatttagggt aatgtaataa
     6301 ataggaggag aaagatgaag cggaaattcc taatcggcag tcacggaaaa cttgcgagtg
     6361 ggttgcagag ttctattgat attctgacag gcaagggaca agagattcag acaatagatg
     6421 cttacattga tgatagtgac tataccaagt ctattgtaga atttattgat gagattgcac
     6481 cagatgaaca ggggcttatt tttacagatt tgttaggtgg cagcgttaat cagaaaatgg
     6541 cgactgctgt aatgaatagt gggaaaaata atgttttttt aataacgaac agcaatttag
     6601 cgaccttgtt gtcattacta tttttgaaac ctgaagagga attaactaaa gaagaaattg
     6661 taaccgttat caatgaatct caagtgcagc tggtagatct cagctttaaa gcgtgttcag
     6721 aagatgactt tttcgattaa taattaggag gaaaaaaatg attactcaaa ttcgtgttga
     6781 cgaccgtttg attcacgggc aagtggcagt tgtatggact aaagaattaa atgcaccact
     6841 tttagtggtg gctaatgatg aggctgccaa aaatgaaatc acacagatga ctcttaaaat
     6901 ggctgtgcct aatggcatga aattattaat ccgttcagtt gaagaatcaa tagctctatt
     6961 taaagatcca cgcgcaactg acaagcgcat ttttgtgatt gtcaattctg ttaaagatgc
     7021 ctgtacaatt gctaaaaata taaccgattt agaagcagta aacgtcgcta atgtaggacg
     7081 ctttgataag tctgatccag ctacaaaagt caaactaaca tcaagtctct tattaaatcc
     7141 agaagagttg gaagccgcaa aagaattagc cagcttacca gatttagacg tctttaacca
     7201 agtattgcct tcaaatacta aggttaactt gagtcagcta gtcaaacaaa gtgaggtatg
     7261 acatatgata atgccagcga cgatggcagc tttagctgtg ttgatttgtt ttgggggaaa
     7321 ttacttaaca ggtcaaagta tgatggagag accacttgta gttggtcttg taacaggaat
     7381 gctactaggt gatattaagg ttggtatctt aatgggtgct tccctagaag cgctattttt
     7441 gggaaacgtt aatattgggg gtgttattgc cgcagagcca gtaacagcaa cagcgatggc
     7501 aacaactttt accattattt caaacattga tcaaaaagca gcaatgactc tagctgttcc
     7561 gattggaatg ttggcagcat ttgttgttat gtttttgaaa aacgtcttca tgaatatctt
     7621 tgcaccgatg gtggataaag ccgcagccgc aaaccatcaa ggtaaacttg tgatgcttca
     7681 ctatggtaca tggattattt actatctcat tatcgcttct atttctttca ttggtatttt
     7741 agtaggtagt gggcctgtta atagttttgt ccatcatatt cctcaaaacc tgatgaatgg
     7801 cttgagtgcg gcgggaggcc tccttccagc tgttggtttt gccatgctga tgaaattatt
     7861 atggacaaat aaattagctg ttttttactt gcttggtttt gttttaacag cttatttaaa
     7921 attaccagcg gttgcagttg ctgccctagg agcagttatt tgcgtcatta gttcacaacg
     7981 tgatatagaa ttagacgcaa ttactagagg tgcgatcagt aaacagacaa cctttgactc
     8041 taaagaatca gaagaggagg acttcttcgc atgaaatcac aggataattt gactaaagaa
     8101 gaccgtaaaa tgcttcgctc agtcttctgg cgctcatgga cgatgaatgc cagtcgtact
     8161 ggggcaacac aataccacgc agtaggagtt atttatactt tactaccagt catcaatcgt
     8221 ttttataaga ctgataagga taaagcagag gcactggtcc gtcatacaac atggtttaat
     8281 gccaccatgc acattaataa ttttattatg ggtcttgttg catctatgga aaagaaaaac
     8341 agtgaagatc ctgattttga cgctagtgct atcaccgcag ttaaggcttc tttgatggga
     8401 cctatatctg gtgttggtga ttcattcttt tggggaattc taagggttat tgcggcaggt
     8461 atcggtatct ccttagcaag tacaggctca gccatgggag ctgttgtctt tctactcttg
     8521 tataacattc cagcttttct gattcattat tatagtctct atggaggtta ctctgtaggt
     8581 gcagggttta tcaaaaaatt gtatgaatca ggtggtatta aaattgttac taagacttca
     8641 agtatgcttg gtttaatgat ggtaggctca atgactgctt caaatgttaa attcaagaca
     8701 atcttaacgg tagcagctaa aggagctaag gaagcagctt ccatacaaag ttaccttgac
     8761 caattatttg ttggcgtagt gccactgttg gtaacgatat tagccttctg gcttctacgt
     8821 aaaaaagtca atatcaactg gatcatgttt ggtattatgg tgttaggtat tgtacttggt
     8881 ctcttaggta tttgctaata atctcctagc attaaaggta agtggaacaa gacgatactt
     8941 gtttagctta tctttttttc ttttgaaagt tattgtatga aaggagttga ctatctttat
     9001 tggatagcta tttgctataa taaaaggaga tggaaatgag aggatataga atggaagaac
     9061 gctttaagaa acgactacaa gatgatatct ctaaacattt ttcccgccag tctttaattt
     9121 tatccttgtt attgattgca cttttcgtcc ttttttcttt agcgcctcag cagataggcc
     9181 tttataaaga tgttaattca gtttcctata gttataagca attgattcaa aaacatgata
     9241 ccttattaga tgacttaggt aaaaatagtt tgaaaccatt tgtctcagga cacttaggaa
     9301 gtgctgattt gagtaagcaa tattatcatt taaggaatca tttacaaagt caaacagaac
     9361 ttttagtatt ctcacctaat caagaacttt tgtttgctag taattcacat ttaggtaatt
     9421 tttttagtaa gtctatttac attagtgaag ttttagataa ggctaaaata aatcaaaggc
     9481 tactaaaaat tattgtagat agtgagggag ggcattacct tgcactcatt aagccaatta
     9541 tagttaataa aaaagtttca ggttatgcct ttttattgat gaatgggaaa gactttttac
     9601 ttccaacgaa ggctattaac tcagacttga ttattgccga tcagctaaat aatagcttta
     9661 cttttactaa tcgtgatttt atctcctcta gtttagataa ggtagatagt caattcttaa
     9721 ctagatattt tagttttcac gatcatcgag cttttgttgt taggaaagta gctctgcaag
     9781 ataatatcct actttatatg tatcgtcccc tgataccagt aactctggtc gtcctttttt
     9841 ctctagtatc atcagttatt atttttgtga ttttacgacg aaaatctaga gttctagccg
     9901 atcgcatcgc agtaaaaaat tctggcgcca taaatcaaat ggtgctagat atggatgcca
     9961 tttcccgcca agaaaaatct agtattgagc ttgatagtca agatgaattt caatatctat
    10021 ctgttcaaat caatcagatg gtttcacggt taaaagattt acatgagaaa actctcgatt
    10081 tggaaacaca aaagttgctg tttgaaaaga ggatgttaga agctcagttt aatcctcact
    10141 tcctttataa tacccttgag acaattttaa taaccagtca ctatgattca caattaacag
    10201 aaagaatcgt tatacaacta acaaaattat tgcgctatag ccttagtggt agcacagagg
    10261 cagctgtgct taaggatgat ttagcaatca ttgaatccta ccttttgatt aatcaagtga
    10321 gatttgaaga attaacttac actatttcag tatctcctga acttgaacac atgcgtgtac
    10381 caaaactttt cttgttaccg ctaattgaga atgctattaa atatggctta aaagaacgtc
    10441 atgatgtagc aataaacata gatatttggc aagatagtga tggaatttgg tttactgttt
    10501 caaataatgg atcgggtatt agtttggcta ggcaaaaagc tatacgtaca atgttaagat
    10561 caaatcactc gcatcatggg cttattaatt cttatagacg attacaatat caattttcaa
    10621 cagtattgtt agagtttacg aagacagatg atgcttttcg agttagctat atagtaaagg
    10681 agtgagttat gtatagatta cttatcgttg aggatgagca ccttattcgt aagtggttgc
    10741 gctatgctat tgattatcaa tcattgaata ttttagtggt tggtgaagca aaagatggta
    10801 aagaaggagc tcaattaatt caggaagagc agccagacat tgttttaagt gatattaata
    10861 tgccaattat gacagcattc gatatgtttg aggcaaccaa aggacaatct tatgccaaaa
    10921 ttattttatc tggctatgct gattttccaa atgcgcaatc agctattcac tatggcgttc
    10981 ttgaattttt aacaaaacca ctagaaaagc aagctttgat tgattgttta aaaacaatta
    11041 tggctagaat tgaggaacat aaggagaagc acctacaaga gcatacagaa ttgtatttac
    11101 ctctaccaca agctaatgac caagttcctg aagttattaa agatatgcta gcatggattc
    11161 atagccattt tcatgggaaa attgtgatta gccagttagc tcacgattta ggttacagtg
    11221 aaagttatct ttacacagtc actaaaaagc accttcatat aactttgagt gattatataa
    11281 atcaatatcg tattaatcaa gccattcagt tgatgtttag agaaccagac ctaatggttt
    11341 accaaattgc agaagctgtt ggtatttatg attatcgtta ttttgatagg gtttttaaaa
    11401 agtacttggg tcagacagta aaagcgttta aggaagaaca tatttttaaa cagatggatt
    11461 aggtaggtga aagatgaaag aaaaacagtc gaaaaggctt atttatatac tactgattgt
    11521 tcccattatc tttataagtg tttttacata cagtattagc cagccttcta aactacttcc
    11581 accaaaagaa ttagttattc taagtccaaa tagtcaagcc attttaacag gaacgattcc
    11641 agcttttgag gaaaaatacg gtataaaagt taagcttatt caaggtggga cagggcaact
    11701 aatagataga ttaagtaagg agggtaagca gttgaaggcg gatattttct ttggaggaaa
    11761 ttatacgcaa tttgaaagtc ataaggcatt gtttgagtct tacgtatcaa agaatgttca
    11821 tactgttatt ccagactata tccatccgag tgatacggcg acaccttata ctataaatgg
    11881 gagtgtcttg attgtaaata acgaattagc taagggactt accatcaaga gttatgaaga
    11941 tttattacag ccttccttaa aaggtaaaat tgcctttgca gatccgaata cttcctctag
    12001 tgctttctca caactcacta atatactctt ggccaagggt ggttacacca atccaaaagc
    12061 gtggaactat gttaaaaagc tacaacataa tattaatgct atcaaatctt ctagctcttc
    12121 agaagtttat caatcagttg cagaaggaaa aatgattgtg gggttgactt acgaagaccc
    12181 tagtgtcaat ttgcaaaaaa gtggtgccaa tgtttctatt gtatacccga cagaagggac
    12241 agtttttgtc ccatcttcgg ttgcaattat aaagaatgct ccttctatga aagaagcaaa
    12301 gttatttatt aattttatgc tttctttaga tgttcaaaat gcctttgggc agtcaacgag
    12361 taaccgacct attcgtaaag atgcccaaac aagtaatggc atgaaagctt taaaggatat
    12421 cgctactctt aaagaagatt atcgctatgt cactaagcat aagagccaaa tccttaaaac
    12481 ctataatcgc attcgtagaa atgctgatta aatgaaaata tagaaaaact cctcggaatc
    12541 attgattccg aggagtttaa tcatcgtcct cgtcgctttc gaaatctttt ttccaataac
    12601 gataaagact aatgcatgcc agaaatccgg ctataatagg aacgataata aataagaggt
    12661 caatcactgt atccatgggc aatatactcc aatgataaga agtcattatt gtttatagtg
    12721 cgtacaataa tgttaaggta aaacgtcggt cattataagt tttttgacat cctctaaact
    12781 catagatttt gttaatattt tttcaggtat ctcttttaag aagtctttct caagccacca
    12841 ccttgcaaga gaatcttctc caaattctaa agatttaagt cgagttgaat gtcttttaac
    12901 agtttcttga aagcttaaat catagtaata agcataagta ttgttggggt tatgttttaa
    12961 aatatgtttc caaacaggcg tataccaatc agaacgaagt atgccttcaa ggatgatgta
    13021 ggaacagtta tggtaaccat aatttagtaa attgatcaaa aggggaatgg tagtagtatt
    13081 ttcaccatcc ttagttccta acatctctct acgaaggtaa tcctgagata agagaagggt
    13141 atcttcccct aattcggctt gaagttgttt agctattgta gactttccgc tagctgaatt
    13201 tcccctgata ataatgagcg tggctaccat tagttcacca tattgctttt gagataggca
    13261 tccactaaga gttcagttgc ttgaatagaa tcgatatggg tacgttcata agaatgacta
    13321 gattcaatac ctgcaccaag tagcgcgtgt ttaacttccg caccagcacg catggcagcg
    13381 gaggcgtcac taccataata aggataaata tcaagtttat aaggaatatt attgttttca
    13441 gctagagaaa caagatgttg acgtaattca taatgataag gaccagaagc atctttaaca
    13501 cagatagaga cagtatattc gtcagtttct tgatcgtctc ccatagctcc catatcaact
    13561 gctagatatt ctacagtttc atttgggatg cttgaatttg ctccatgtcc tagctcttca
    13621 aaggcactaa agtagaaatg agtagtataa ggaagttgaa tgtcttcttt cttgtaaaca
    13681 gaaagaagtt ccattaggat accagcggat accttgtcat ctaggtaacg tgatttaata
    13741 aagccgctgc ctgttatgat tgtacgcgga tcaaatgaaa tgaaatcacc gacctggatg
    13801 cctaaagcac gtgtttcgtc agcagttgtt actttctcat ctaaacgaat ttccatattt
    13861 gtttgattac gttcagcagt tccagcatct ttgtaaacat ggacactagt ttgatgaata
    13921 agagcagttc cagaaatttc tttaccattt tttgagaggt gtatagtaca gttttctcct
    13981 tcaatggcat tgtatgtgta tccgccaata aggtcaattt ttaaccgacc atctggtttg
    14041 atggccctaa ccatagcacc aagtgtatca aggtgagcag ttatcatgcg atgtttagta
    14101 ttattttttc cttttagaga aaccatgacg cctcccttgt ttgtgcgaat tggtgagtag
    14161 ccaaacgctt ccaattcttt gatgatatag gtcatgattg tttgggtaaa ccctgttggt
    14221 gaagggattg atgtcagggt tttaatgtag tttaatgttg tttccatact atttccttta
    14281 ggctttaaag ttcgataatg tttcaacgat aatatttgcg atacgttcaa cgtcgctaga
    14341 agttcctcgc aattcaaagt ctcctaacat aggaaatcca aaccgttcag agtattgttt
    14401 tgcagttaaa cagtactgat tgttaaaatt tctatttcca gagccaacaa ttccgataca
    14461 atttttgaca ttattgtgtg cagctataaa atcaccaagt ggaatggtaa gaatttcttg
    14521 atcgccattg tcaatgccat ttcctccctc gaggtaagtg ggtaatagtg ctacgaaagg
    14581 ttggtcaaca ggaaaggttt gatgattttg ttccttgata ttaatagtcg aaacctgaca
    14641 gttatgccaa gttgacagtt gttcactaag acgtttgaca aagctttgag tattgccgct
    14701 aagactgata tagactaggg ttaatttagg catttagtac tccttaaatc taaatttctt
    14761 gttatcagtt tagcaaaatc atgtcagata aacaagctag agtggtatct caatatctga
    14821 ctgtttagag agaaaatcta ttgttgagac taaaaactga ggttgattgg cctcagtttt
    14881 tagtgatgac tatatttgtt tctttttaag taaatttccc acgagtccaa gtattgcgac
    14941 gccaaagaaa gccagataag attgtttttc ttgtccagtt ttaggtaatg ttttttcttt
    15001 ctgttgtgta tagatagtgc ctgcgatatt tggagaagct tttgcttttg tataagcagt
    15061 tgcaaccatg tggtgaattt tttgtttctc tatttggctt aacttttctt gatgtgatgg
    15121 ctcagtaagg tctacttttt cttgttcagt atagacaaat cgataatcac caaatccttc
    15181 atctttggta gaactagcga tatactgaat atccttgcta ttagtaacaa gatttttagc
    15241 tttatcggct gtttggaatc gtaaatcaag gcctttaata gactctgtga agccccagtt
    15301 attatcagct gtaggattga tggttttctc tgaaataata tagtttataa tagtttggcg
    15361 attttctaag ttcagcaaac ggttcattgt ggcattttta gccccaggga aggtaccact
    15421 agcacggtag ttattggtca caatcataaa ctcttgtttg tctgcaacag gcttaccatt
    15481 gtacattaaa tcgcgtactc gactagcttg actattaaca acttttccat ctttgttgta
    15541 tttattaggt tgtgtaatat caaatttata agtcaagcca tcaataacgt caaaattata
    15601 ggtacgatat tcggtattga taatattttg cggtgcttta ttgttagggt caatttggtt
    15661 aaattgacca gcggacattt ctaaccattc acgtaagtct gctccggtca cttttaaaag
    15721 tgcagtaaca ttatcataaa ggtaaagatc agctacgttt ttaattgcaa caggtccagc
    15781 tggaatatca gtatatgcgg tagcatcgcc acgtgttccg gctttaaatg gtgctgcagc
    15841 agatagaagt ggtaaatttg cctcaggtgt tccttttaat tcatttgcaa cgtaccaacg
    15901 ttgagcgttg ttaacaattt gaactgaagg gtcatcttta actagtgcaa agtaacttgt
    15961 aattggcgca gttgttgtac ctacttgttg gcgaacatag ttgatagtgc catcgtgtgc
    16021 ttccttagcc aatgcaatga tacgctcgtc agcagttgtt gagttcatat caattttacg
    16081 gattttagca ctgctttcgg agacttgcca ttttccatta gtataactaa gtcctaaatc
    16141 aataatacca aggtgatccc cgtacttgcc tgccattgta acaggtgttc catttatttt
    16201 tccattgata ccatcaactc cagtgtattt ttcatagaag ccagtaccgt tacctgatgg
    16261 aaattcagcg tgtgagtgtc ccgtaacaac ggcatccact cccttgatgc tggcaatttg
    16321 atagccaacg ttttcttcac cttcttcata tcgatcatct ccaatgccag agtgtgataa
    16381 aacgagaatg acatctgctc ctttagcacg catagtagga acgatttctt cgattgcttt
    16441 aactgaatct ttgacaataa cctttccctc taagttagcc ttgtcccagt ttaagatttg
    16501 aggaggtaca attcctgtaa ttccaatatt tagtgcaaca gctcttcctg ttgaatcttt
    16561 gaaggtttta gtaatgatgt cataagtttt aaaaactggt tgatgtgttt tgaaatccaa
    16621 aacatttgcg ttgaggattg gaaggttggc tgtagcaatg atttttttga ggtaatctaa
    16681 accgtaatta aattcatgat tacctagtgt tgatgcatca taaccaagag cattcatggc
    16741 ttggtacata ggatgttctt cattttctgc gacaggttta acgattgctt tataagttcc
    16801 taaaggtgtc ccttgaatga catctccgtt atcaactaag acggtattag gattttcttt
    16861 ttttgcttct tcgataagga cagcagtttt tgctaaccct attttttgag attctttatc
    16921 ttgataataa tcatagttaa ctaaattggt gtgtaggtct gtggttgaca tgatgcgaag
    16981 gtcaactttt tcaccttcaa taggtttgag atcaccagtt gtggtttctg tggcaacagg
    17041 ctgtggattt tgattattgg cagtggtatt tgtgcttgtg gctgttgaag catcagttat
    17101 tgcagttgga gtagaagtaa cgtttgactt ttcctctgca attgtgctag tgggggtagt
    17161 tgattcaacc tttgtagcgg ttgaacttgg tgttgtaaca atgtcttctg cttgaacagc
    17221 taaaccactt gttgttgtag ctgttaagac agttaaagcg aatatgcttt ttgaaaaata
    17281 atgttttgac ataaatctcc tccattaaaa cattacatgt atattatcct aatctaaaaa
    17341 ctattatagc ttattgaaaa cgtttaatca atctcattta cgaattattt ttagtaatta
    17401 cactaaaaac aatcgtttat ttataaagga taacttggtt agacacattt acttggtatt
    17461 taattctttt tgagcctcta acttctttta tttttaggtt aaaaagtgat aaaataggta
    17521 caatctatca ctatgattaa agaggataac tatggtcaaa gaaattaatt taacaggaga
    17581 agaggttgtt gcaatcacat cccagtacat gagtgagacg gatgttgctt ttgtaaaatt
    17641 tgccttaaat tacgcaaccg cagctcatta ttatcaagca agaaagtcgg gcgagcccta
    17701 cattattcat ccaattcaag tagcaggtat tcttgcagat ttacaccttg atgccgttac
    17761 ggtagcttgt ggttttttac atgatgttgt agaagatacg gaaattaccc ttgatgagat
    17821 tgaaactgat tttggcaagg atgttcgcga tattattgac ggtgtaacaa aattgggtaa
    17881 agtggagtac aaatctcatg aagaacagtt agcagaaaat caccgtaaaa tgttaatggc
    17941 tatgtctaag gatatccgtg ttattttggt taagctcgct gatcgcttac ataatatgag
    18001 aacccttaaa cacctcagaa aagataaaca agagcgtatt tcacgtgaga ctatggaaat
    18061 atacgctcct ttagcgcatc gtttggggat tagtcgtatc aagtgggaat tagaagattt
    18121 atcttttcgt tacttgaatg agacggaatt ttataagatt tctcatatga tgagtgaaaa
    18181 acgtcgtgaa cgcgaagagt tggttgatat aattgtcgat aaaatcagat cctatactga
    18241 ggaacagggt ttatatggtg atatttatgg tagaccaaaa cacatttatt ctatttacag
    18301 aaaaatgcgt gataaaaaga aacgctttga tcagatttat gatttaattg cgatacgctg
    18361 tattatggaa actgctagtg atgtttatgc catggtgggg tatatccatg agttatggcg
    18421 tccgatgcca ggaaggttta aagattatat tgcggcacca aaggccaatg gttatcaatc
    18481 tattcacacg acggtttatg gacctaaagg gccaattgaa attcagattc gaactaaaga
    18541 aatgcatcaa gttgccgagt ttggtgttgc agcccattgg gcctataaaa aaggaatcac
    18601 cagtaaggtt aatcaagcag agcaatcggt tggtatggga tggattcaag aattagttga
    18661 gcttcaagat gaatcaaaag acgccaagga ttttgttgac tctgttaagg aagatatctt
    18721 tacagaacgc atctatgttt ttaccccaaa tggtgctgtt caggaattgc caagggaatc
    18781 tggaccaatt gattttgctt atgctattca cacgcaagtt ggagaaaaag ctactggcgc
    18841 taaagtaaat ggacgtatgg ttccattgac tgctaagtta aaaacaggag atgttgttga
    18901 gattatcaca aaccctaatt catttggacc aagtcgtgac tggattaaga ttgttaagac
    18961 caataaagct cgcaataaaa ttcgtcagtt cttcaaaaac caagataaag aaacttctat
    19021 taataaaggt agagaattac tggttgatta tttccaagaa caaggttatg tgcctaacaa
    19081 atatttagat aagaaacata ttgaagaaat ccttccacgt gtcagtgtta aaagtgagga
    19141 agctttgtat gcagccgtag gttttggtga tttgagtccg attagtattt ttaataaact
    19201 gacggaaaaa gaacgccgtg aggaggaacg tgctaaggct aaagcggaag cagacgaact
    19261 tattaacggt ggcgaaatca aaaccgataa gcgtgatgtt ctcaaagtta agagtgaaaa
    19321 tggtgttatc attcaaggag cttcaggttt attaatgcgt attgcaaaat gttgtaatcc
    19381 cgttcctgga gatctcattg aaggctatat aactaaaggt agaggcgtgg ctatccatcg
    19441 ttcagattgt caaaatttaa agagtcagga gaactacgaa caacgtttga ttgatgttga
    19501 gtgggatgat gatggatcta aaaaagagta catggctgaa atcgatattt atggtttgaa
    19561 tcgtagtggc ctccttaatg atgttcttca aaccttatca aatgcaacaa agctagtatc
    19621 gacagtaaat gcacaaccaa caaaagatat gaaatttgct aatattcatg ttagctttgg
    19681 aatttcaaat ttagcacaat tgacaactgt ggttgataaa atcaaaatta tcccagatgt
    19741 ttattctgtt aaacgtacga atggttagga gagcaaaatg aaaatcatta ttcaacgtgt
    19801 tagtcaagca agtgtttcta ttgaagatga tgtagtcgga tctattgaga agggtttagt
    19861 acttctagtt gggattgctc cagaagatac aacagaagat attgcttatg cagttcgtaa
    19921 aattactagc atgcgtattt tttctgatga tgaggggaaa atgaaccttt caattcaaga
    19981 cattaaagga agcgtcttat ctatctcaca atttacactc tttgcagata ccaaaaaagg
    20041 aaatcgtcca gcttttactg gggcagctga tcctgttaaa gcgaatcagt tttatgatat
    20101 ctttaatcaa gaattagcaa atcatgtttc ggtagaaaca ggtcaatttg gagctgatat
    20161 gcaagtcagt ctgattaatg atggaccagt aactatcgtt ttagatacta aaaataaata
    20221 aaaaatcaaa gagttgggca aaatgcttgg ctctttttat tgtggttcta attatactcc
    20281 ttaacccatt catgtttata aaaagattca ctgtagcttg gaaaggcatg aataaaggtt
    20341 gttgatatga aattaagtgc acttaacaat attaaaaata gaaaaatatt agatttatgg
    20401 tttttcgttg acactaaaat aagaatactc taaaacgtta actggttaag tactttttat
    20461 tttaaaggag gtttaatgaa aaggatacgg aaaagcctta tttttgttct cggaatagtt
    20521 accctaattt gcttatgtgc ttgtactaaa cagagccagc aaaaaaatgg cttgtcggtc
    20581 gtgactagct tttatccagt atattccatt acaaaagcag tttctggtga tttgaatgat
    20641 attaaaatga ttcgatcaca gtcaggcatt catggttttg aaccctcatc aagtgatgtt
    20701 gctgccattt atgatgctga cctatttctt tatcattcgc acacactaga agcttgggcg
    20761 agacgtttgg aacctagttt gcatcactct aaagtatctg taattgaagc ttcaaaaggt
    20821 atgactttgg ataaagttca tggcttagaa gatgtagagg cagaaaaagg agtagatgag
    20881 tcaaccttgt atgaccctca cacttggaat gaccctgtaa aagtatctga ggaagcacaa
    20941 ctcatcgcta cacaactagc taaaaaggat cctaaaaacg ctaaggttta tcaaaaaaat
    21001 gctgatcaat ttagtgacaa ggcaatggct attgcagaga agtataagcc aaaatttaaa
    21061 gctgcaaagt ctaaatactt tgtgacttca catacagcat tctcatactt agctaagcga
    21121 tacggattga ctcagttagg tattgcaggt gtctcaaccg agcaagaacc tagtgctaaa
    21181 aaattagccg aaattcagga gtttgtgaaa acatataagg ttaagactat ttttgttgaa
    21241 gaaggagtct cacctaaatt agctcaagca gtagcttcag ctactcgagt taaaattgca
    21301 agtttaagtc ctttagaagc agttcccaaa aacaataaag attacttaga aaatttggaa
    21361 actaatctta aggtacttgt caaatcgtta aatcaatagg tagttatata aagaaaggac
    21421 gcaggatgaa tcgtaaaaaa acagttatta ttagtgcttt atcagtagct ctatttggta
    21481 ctggtgtagg tgcttaccaa ttaggtagct ataatgccca aaaatcagac aatagtgttt
    21541 cttatgtgaa aacagataaa tctgatagca aagcacaagc aacagctgca aataaaacgc
    21601 ccgaccaaat cagtaaagaa gaaggcatat ctgcagaaca aatcgttgtt aaaattactg
    21661 atgatggtta tgtaacttct catggagacc attaccatta ttacaatggg aaagtgccat
    21721 atgatgccat cattagtgaa gaattgatta tgaaagaccc tagctatgtc tttaataaag
    21781 ctgatgtcat taatgaagtc aaagatggtt atattatcaa agttaatggt aagtattacc
    21841 tctatcttaa agaaggtagc aaacgaacaa atgttcgtac aaaggaacaa atccaaaagc
    21901 aacgcgaaga atggtctaag ggtggttcta agggagaatc aggaaagcat agtagtgcaa
    21961 aaactcaagc tttatcagcc agcgtacgtg aagcgaaagc tagcggtcgc tatactacag
    22021 atgatggtta tgtttttagt ccaacagatg ttattgatga catgggagat gcctttttag
    22081 taccacatgg cgatcatttc cattatatcc cgaaagctga tttatctcca tcagagttat
    22141 cagccgcaca ggcatattgg aaccgcaaga ctggccgatc aggtaatagc tcaaaaccat
    22201 caaattcaag ttcatacatt catgcaagtg caccaagcgg taatgtatca acaggaagac
    22261 atgctaatgc ccctatttct attccaagag tcactcacgc aaatcactgg tcaaaaccag
    22321 ctggtaatca tgctacggca ccaaaacacc atgcacctac aaccaaacca atcaacaaag
    22381 attctgcttt agacaaaatg ttgaaacgtt tatatgctca accgttatct gctcgtcacg
    22441 tagaaagtga tggtttggtt tatgacccag cacaagttaa tgctttcaca gcaatcggtg
    22501 tttctattcc gcacggtaat catttccact ttattcacta taaggatatg tctccattag
    22561 agttagaagc aacaaggatg gtggcagagc atagaggaca tcatattgat gcattaggga
    22621 aaaaagattc tacagagaaa ccaaagcata tttctcatga acctaataag gaacctcaca
    22681 cagaggaaga acaccatgca gtaacaccga aagaccaacg taaaggcaaa ccaaatagcc
    22741 agattgtcta cagtgctcaa gaaattgaag aggcaaaaaa agctggtaaa tacacaacat
    22801 ctgatggtta catttttgat gctaaagata ttaaaaaaga tacaggtaca ggttatgtca
    22861 ttccacatat gacacatgag cattgggtac caaagaaaga tttatcagag tcggaattaa
    22921 aagcagctca agaatttctt tcaggaaaat ctgaagcaaa tcaagacaaa ccaaaaacag
    22981 gtaaaacagc tcaagaaatc tatgaggcaa ttgaaccaaa agcaattgtt aaacctgaag
    23041 atttattatt tggaattgca caagcgacag actataagaa tggtacattt gtaattcctc
    23101 ataaagatca ttaccattat gtggaattaa aatggtttga tgaagaaaaa gatcttttag
    23161 ctgattcaga taagacatat tctttagaag actatttagc tacggctaaa tattacatga
    23221 tgcacccaga aaaacgtcct aaagttgaag gatggggtaa agatgctgaa atttataagg
    23281 aaaaggactc taataaagca gataaaccaa gtcctgcacc aactgataat aaatcaacat
    23341 caaattctag tgacaaaaac ttaagtgctg cagaagtatt caaacaagca aaaccagaaa
    23401 aaattgtacc gcttgataaa attgctgctc acatggcata tgcagttgga tttgaagatg
    23461 atcaattgat tgttcctcat catgatcatt atcataatgt tcctatggca tggtttgaca
    23521 agggtggttt atggaaagca ccagaaggct atacattaca acaactcttc tcaacaatta
    23581 aatactacat ggaacatcct aatgaattac caaaagaaaa gggttgggga cacgacagtg
    23641 atcataacaa aggctcaaat aaagacaata aagccaaaaa ttatgctcca gatgaagaac
    23701 ctgaagattc agggaaagta actcacaact atggttttta tgatgttaat aaaggttcag
    23761 acgaagaaga accagaaaaa caagaagatg aatcagagct agatgaatat gaactaggaa
    23821 tggcacaaaa cgctaagaaa tatggtatgg atagacaatc ttttgaaaag caactcatcc
    23881 aattatcaaa taaatatagt gtaagttttg aaagctttaa ttatattaat ggtaatcaag
    23941 ttcaagttac taaaaaagat ggctctaaag tgcttgttga tattaaaaca ctagcagaag
    24001 tgaaataata aaagagttga ataaacttag ctgacaacta aaaacaaaca agtcaccgca
    24061 cttgtttgtt ttttagttat gatttctagc gtgcgaagat tttaatgata tatctaattc
    24121 ttcttttgaa caaccattgt tttacgctta aaagagagtt aatgtatttt tgtttgagga
    24181 agttttcttg ttttgacttt tataaaacga ctaaagttat aatcactaaa taaagagcca
    24241 ctactaaaag ctgtataaca ggtttctagt agtggctcac ttgttttatt acatgtaatt
    24301 gttattagtt aaaattttgg tgaagctact gtattgctcc ttaacaacat tatcaatatt
    24361 atgatctgtt aatacaaaat caatatcgtc taattgataa aaagtataaa aatcatattt
    24421 accaaatttt tggctatcaa ctaaaaggaa tttttcaaag gaattattaa gagcaatgcg
    24481 ctggatttca ccttctccct cgtcataagt agcgatagac ttttcaaaaa caccattact
    24541 actaacaaaa gcttttgaaa aattaagtga tttaatagag ttaatagtaa cagaaccgac
    24601 aaaagcacca gtaatttctc tgtactctcc tcctacaagg ataaggtcaa gtgttgaact
    24661 ttgattcaga atattgaaaa caggaaggct attggttact accctaattt taaaatcaag
    24721 taattgcgta gctaatttct ctaaggttgt tccaggccca ataaagattg tttcaccatc
    24781 attgatgaat tgttttgcaa attgggcaat ttctaatttt tcatttgttt gaatatcgta
    24841 tttttcagtg tttgattttt cataattttg tggaattggt gctgcgttta ctttttgtgc
    24901 accaccatgg atgcgtatta acagtcctgc tttatctaat tcatccaagt cacgcctaac
    24961 agtcatgtct gatacatcaa gttcttccat gatttcatta acagtgacaa taccatttat
    25021 attgactttt tctattattt tttgtaatct ttctcgcttt aacataataa tctctcctac
    25081 ctcttcattg tatatatcat accataaata gagctaaaat aataataatt ttgaaaaaac
    25141 aaacaataat aaacaaaaat aaataaaaaa cgtcttgtga tgttttattt tgttttgtat
    25201 aatattgatg tagataagtt gataaggctt gtttgcctta caacaaaatg taaccgctta
    25261 actaaaaaga aaggtgtttg ctgtgactca agatatttta tttattgatg cccattctca
    25321 ggaagaatta tttgacttag tatcaaaagc attgataaaa caacactatg ttagtcctga
    25381 ctaccgacag gcagtaaaag aaagggagag agagtttcca acggggttaa aaattgattt
    25441 aaaagatggg actcctattc aatatgttgc tattccacac actgaaacgc agtattgtct
    25501 agtagataga atcttttatg tcaaaaatag tcagccaatt actttcaaac atatgattaa
    25561 tccagaagag gagtgtaggg ttcaggattt tttcttcatc attaatagta ggaatagtaa
    25621 ccaaagtgat attttatcta atttgattac cttctttatt acaaaaggaa acctagatcg
    25681 gctacatgag cttggcgaca ataaggaaaa aattaatcat tatttaatag aaaaaggagt
    25741 gttttaacat gatcaaaata ttagcagctt gtggtgcagg cataaattca agtcatcaaa
    25801 ttaaagatgc tatcgagaca caattaggag atagaggtta caatgtacat tgtgatgctg
    25861 ttatggttaa ggatatcact gaggaaatgg tcaacaaata tgatatcttt actcctattg
    25921 ctaaaactga tttaggtttt aatgtaccaa ttccagttgt agaagcagga tctattcttt
    25981 atcgcatacc agttatgagc gaaccggtct ttactgcttt agaacaagtt atcaaagaac
    26041 acaacctatc ataacatatt taaaattgga attagtctta aaggagaagg aacatgcagg
    26101 tatttttaaa tattgtcaat aaattctttg atccagttat tcatatgggt tcgggagttg
    26161 tgatgctaat tgtcatgaca ggtttagcca tgatatttgg agtgaagttt tctaaagcac
    26221 ttgaaggtgg tattaagtta gctattgctc ttacgggtat tggtgctatt attggtattt
    26281 taactggtgc tttttccgaa tcacttcaag cttttgttaa aaatacagga atcaatctaa
    26341 gcattattga cgttggttgg gctccattag caactattac atggggatca ccatatacgc
    26401 tttacttctt attaatcatg cttattgtca atattgttat gattgttatg aaaaaaaaac
    26461 ggatacctta gatgttgata tttttgatat ttggcattta tctatcacag gtcttttaat
    26521 tatgtggtat gctaaacaaa tgaaacgccg taatgcagaa tatgcagcta atcaaaaata
    26581 aatagaataa acaaaaacct aggaaatatc acattctcct aggtttttga tatattaaat
    26641 attttaaaaa taatatatat aattaatttt cgctcatttt aataataaaa aactagtaaa
    26701 attataaaaa tatattgaaa atagtgataa gttggtatac aatccttttt ggaaacatta
    26761 ctgaactccc atttttctag agttgatgta tgcaaccaat attttaagac ttggagtttc
    26821 tatgacatta acctttggga aaccttctat aacgatacgc cgtctaggag tgataactag
    26881 tacatatatg atgctaggtt ttatcttatt atgtggactt ttgcatccta gtacaattta
    26941 tagtgaggaa ataaaatctg atataaactc ttttatatca atagatgatg aaaaaactac
    27001 tgctcccata tttgactcta ctttagataa gcttactaat tcaacagagg agaggcagtt
    27061 agaattagat tctgatacat cggacggttt aatagtagat gatcaaaaag ataaagatat
    27121 ggtagtagtc ttaggtgatt ctgacagtca agaaaaattg tcacctcctc taaaatcgga
    27181 aatagcgaca gaagaaaatc gtaaacaggc aaatttagca gaactaaatg caactcaacc
    27241 taataataga actacctata ttatacccga aagcagtcat tccattgcag aacaacagag
    27301 attcctgata gaatcaaagg gttcttcggt tgcattactt aatagcgatg aatttagaaa
    27361 gacagcggga gaggatagag gttttgaaag ggataagttg aggtctttgg atatcattcc
    27421 taagggagat ttatcgacaa gtaatgtcat aggtaatacg gacattgcta gtcagatatc
    27481 gttgggcttt aaaaagaatg cgatgcagga acaccatctt actaaaacat tctctcaaaa
    27541 ggatggaaag ttatcgtctg ttatagaggg gatgcttgct attggcaaag agaaagtaga
    27601 gaaagaaata aaatatagtg gtaatttatg gcaaaaatta aaagctaagg cacactgcct
    27661 tgtttgctgt gttgataatt tgaattttga agatataaaa tcttattttc aatattattg
    27721 tcatctaaac catcagctca aattacctaa aggtgctata ctttctgcta aaacagaagt
    27781 atatagggga ggagattttg ggagaaaaaa taaagataat gtgtttggtt accgtatccc
    27841 ctcattattg aaaacccaaa aaggaacttt acttgcggga gctgatgaaa gaattgagca
    27901 agcttgtgat tggggaaaca taggaatggt tattcgccgt agtgaggatg atggtgtcac
    27961 ttggggaaaa agagaaacta ttgtcaatct ccgtaataac cctagagttc cgctagttac
    28021 tagtggtgac tatagtggct cacctattaa tatggatatg gcattagttc aagatactag
    28081 ctccaagacg aaacgtattt tttcaatata tgatatgttt ccagaaggaa gaggcgttat
    28141 tagtattgct aacacacctg aaaaagaata tacccaaatc ggaggacagt cttatcttaa
    28201 tttatataat aatggaaaga aatcgaaggt ttttactatc cgtgacaaag gtattgtata
    28261 taattttaaa gggaaaaaga ctgattatca tgttataaca gaaactacta aaagtgacca
    28321 ttcaaatcta ggggatattt ataagggaaa acagctactt ggaaatatat attttacaaa
    28381 acataaaacg tcaccatttc gtttagcaaa atcaagctat gtgtggatgt catatagcga
    28441 tgatgatggt aggacatggt catcacctag agatataaca gcaagtcttc gtcagaaagg
    28501 catgaaattt ttgggaatag gacctggaaa aggtatagtt ttaaaatggg ggccacacgc
    28561 tggtcgtatt attattcctg cctattctac gaattggaaa tctcatctaa gaggttcaca
    28621 atcttcacgc ctaatttatt cagacgacca tggaaaaacg tggcatactg gaaaagcagt
    28681 taatgataac cgtatacttt ctaatggtga aaaaattcac tccttaacaa tggataataa
    28741 aaaagaacaa aatacagaat ccgtacccgt tcaattgaaa aatggggaca ttaagttatt
    28801 tatgaggaat ctaactggta acctagaagt agccacaagt aaagacggcg gggagacttg
    28861 gcaaaaccat gttaaacgat ataaggaaat tcatgatgct tacgtccaac tatcagctat
    28921 tcgctttgag catgacaaaa aagagtatat tttattagtg aatgctaatg agccagggaa
    28981 gaagtgccaa gatggatatg cacgtctagc gcaagttaat cgaaatggta gttttaagtg
    29041 gttatatcac catcacattc aagatggttc gtttgcttac aactcgttca acaacttaat
    29101 aatgatcaat ttggtgtcct ttatgaacat agagaaaaac atcaaaatag ttttacttta
    29161 aattacaaag tttttaattg gagttttctt agtcaaaata cagagaagca aggcacttta
    29221 tgggagaaaa tggcagcaaa ttggcatgtt ttgtttaaat tttatttatg attatggctt
    29281 gcggaataac cgttcctaca aataaaaata atgagaagta tcaaacttcg tgatagctag
    29341 ctagtagtga attctagttg ccatagttat cacggagttt ttttgtttat ttcaaacaaa
    29401 aacaaaaaca aaacattgac aaaaacaaaa ataagatata tgatgatatt acaaacaaaa
    29461 aacaaacaat atcttccaac tacatacaat tgtattaaaa gtgaaataag gagaaagaaa
    29521 tgacaattat tattggggcg gatgcgcatg gtgttgaact taaggaagtg attagacaac
    29581 accttacacg tttaggtaag gaaattattg atttaacgga tactagcaaa gatttcgttg
    29641 ataatacctt agcaatcgtt gctaaagtga atcaaaaaga agataactta ggaataatgg
    29701 ttgatgctta cggtgctggt ccttttatgg tagccactaa ggttaagggg atgattgcag
    29761 cagaagtttc agatgaacgc tcagcttata tgactagagc tcataataac gctaggatga
    29821 ttactttagg ttcagagatt gttggtccgg ggttgctaaa catatagtag agggttttgt
    29881 tgatgggact tacgatgcag gtcgtcatca aattcgggtc gatatgttga ataaaatgtg
    29941 ctaagcgtaa gagaggtaaa gcagatgaaa attgcggtcg gttgtgatca tattgtaact
    30001 tatgacaaaa ttgcagttgt ggattattta aaaacaaaag ggtatgaggt tattgattgt
    30061 ggaacttatg ataacatccg tactcactat cctatttatg gtaagaaagt aggagaagct
    30121 gtcgctagtg gaaaagccga cttaggtgta tgtatttgtg gaactggagt tggtattaat
    30181 aatgctgtta ataaagttcc aggaattcgc tcagctctgg tacgtgattt gacatcagct
    30241 atctatgcta aagaagaatt aaatgctaac gttatcggat ttggtgggaa aattactggt
    30301 gggctgttaa tgacagatat catcgaggcg tttataagag ctaaatataa accaacaaaa
    30361 gaaaacaaag tattaattga aaagatagcc gaggttgaga ctcataatgc ccatcaagaa
    30421 gagaatgact ttttcacaga gttcttagat aaatggaatc gtggtgaata tcacgactaa
    30481 ggagatatct atgattttaa cagtaacttt gaacccttct attgatattt cgtactgctt
    30541 agaaaacttt aacatggata ctgta
//
LOCUS       DATRSG010000018        29580 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRSG010000018 DATRSG010000000
VERSION     DATRSG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29580
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.018"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(673..849)
                     /locus_tag="VBD89_000782"
     CDS             complement(673..849)
                     /locus_tag="VBD89_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135329.1"
                     /translation="MAYPDKSFKPTPINELIEKFDKEINQFMENQKEVISVNTQTNGL
                     AGGFQYFVTVVYRN"
     gene            complement(878..1255)
                     /locus_tag="VBD89_000783"
     CDS             complement(878..1255)
                     /locus_tag="VBD89_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO7135330.1"
                     /translation="MSKKNAMRKLKEFQRWQRIANSLDLTYNELYQFDIEYHSTRRTY
                     LEISRECALEELGAIKYAINQLSKIDYRQILIECYLISEKLSNQEIMTKLNRSESWYY
                     ETKKRALLEFAEHYRDGILNKIN"
     gene            complement(1230..1592)
                     /locus_tag="VBD89_000784"
     CDS             complement(1230..1592)
                     /locus_tag="VBD89_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO7135331.1"
                     /translation="MGAKEQLKELKPLFALMTLFEEQRDKDIKLMNAFRNPELLNGIE
                     KGTAQQLLCLAKERDKRLAMITSLQDENQIAVIKARYVDDLSWDEIPDKVGCSRNTVF
                     KLHREALEVLDEQEERHA"
     gene            complement(1993..2481)
                     /locus_tag="VBD89_000785"
     CDS             complement(1993..2481)
                     /locus_tag="VBD89_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135332.1"
                     /translation="MLELAIESIIKPMKAQRKTKITGTMDNQPIRIDLDNLVIHYNHQ
                     NLLLETIPGTYGGKRYFFLCPKCERRCRKLFKASHDFACGSCQKVHQATLNRSKTDCC
                     YYWRLAFKECLKVDPKAKHKHGYYSHDDFPKRPKYMRIAKYLYHWKRFHYYMDKGDRH
                     WL"
     gene            complement(2555..3094)
                     /locus_tag="VBD89_000786"
     CDS             complement(2555..3094)
                     /locus_tag="VBD89_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135333.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGTYHAPVIG
                     VTIKGKGQLVLEYVEDKKLSKVFKRICDSFKDYYNQSEDMTDEELDDMAQENELIKEV
                     EELDDQRVVPLFEITQEEANEQDTLMAFISDHDQLAEHLSMNYQEMSQDDLGAILETI
                     SQAFNHLYDMVVEGQLLVK"
     gene            complement(3096..3269)
                     /locus_tag="VBD89_000787"
     CDS             complement(3096..3269)
                     /locus_tag="VBD89_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135334.1"
                     /translation="MKLQLHTRGGNTITIQGDRTLYNELVKYLLSGQQPNWVACPSAI
                     INLSDIIAITKEK"
     gene            complement(3275..3493)
                     /locus_tag="VBD89_000788"
     CDS             complement(3275..3493)
                     /locus_tag="VBD89_000788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135335.1"
                     /translation="MKIKLFNRKRIETGFNEYMDLPKFRNETNEEFENRVNSFIADKK
                     VIDIKYQEATYGNYEDMSTTTSLLVLYR"
     gene            complement(3768..5393)
                     /locus_tag="VBD89_000789"
     CDS             complement(3768..5393)
                     /locus_tag="VBD89_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO7135336.1"
                     /translation="MKSLESEILEARESEDKYFKTFKSVRGQLIKECQSIKEEAYQIA
                     YDEVMADSEHLENVKAGRLTEVQHKELAHQKGQEASEKALPKTPLGVAIMLKQYIHFI
                     RIKPEAQGQKAPLYYYNPDFGIWLEDNELLQDLISTIFPNATEKQAFDTLYKIARQSQ
                     MKEIQGKYTVIGKQLFNNKTGEFEPFNPKIIVTRKIKTAYNPYAKEPTINGWKVTEWL
                     KELFDGDKELYQLAIQIIKASITGQSLEKIFWLYGEGGTGKGTFQQLLINLVGLENVA
                     SLKITDLNKSRFTTSILLGKSLVIGDDVQKDAIIKDTSDMFSLATGDIMTIEDKGKKP
                     YSLRLNMTVVQSSNGLPRMNGDVSAIDRRFRILIFSSRFKDKPNPAIKKDYINRKEVL
                     EYLVKLAIETPSEDINPKQSQTLLGEHQKDINPVLDFVEKTFREDLASEFIPNDFIWY
                     CWKQYQDYFNQSFFKTERGLHRDIKQVLPPYIRTGVRTIPSGRQLHLGFYPKEDTPDY
                     ASLATYSNGRETPEKRKRPKQDRGYWNSKAKHK"
     gene            complement(5413..6261)
                     /locus_tag="VBD89_000790"
     CDS             complement(5413..6261)
                     /locus_tag="VBD89_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEO7135337.1"
                     /translation="MAIYEARGFSSYLYPYKGQLEPFDYIAQFKPLKPPEGINIEEYK
                     RTQAPYCLSGKVTAEKNGGYKRNNASLVYRDLIFLDYDEIETGANLPKIVSKTLWEYS
                     YIIYPTIKHTPEKPRYRLVVKPSDTMDKTTYKQVVKEIANKIGLPFDLASLTWSQLQG
                     LPVTTGDPEDYQRYVNRGLDYPVPKAPNRQVTTTYTSRPRSQRSITMRVIDTLFNGFG
                     DEGGRNVALTKFVGLLFNKWVDCDIETAYELTKIANGVTDNPLPIEELDRTFTSIARA
                     EFRKRG"
     gene            complement(6262..6534)
                     /locus_tag="VBD89_000791"
     CDS             complement(6262..6534)
                     /locus_tag="VBD89_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135338.1"
                     /translation="MSLFSQDYEAKLLEQNLTAFNRFLEAYQKPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLRRYQPPLEDTRKHYYKVSDILIFWGVNV"
     gene            complement(6537..6866)
                     /locus_tag="VBD89_000792"
     CDS             complement(6537..6866)
                     /locus_tag="VBD89_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135339.1"
                     /translation="MTLPENYRRVLNLIKVGADNPITGAEISLILKLEERSVQSIISS
                     LITRYNIPIIGIRHGFNRGYFIPANKEELLDGAKAFYNQVQKEQERLSVLLNADLTSY
                     KELLKGG"
     gene            complement(6863..7225)
                     /locus_tag="VBD89_000793"
     CDS             complement(6863..7225)
                     /locus_tag="VBD89_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135340.1"
                     /translation="MTTEEYFKKLLEDSEKQPTNWLSDRVLDKNIKMLDEDIEAEWDD
                     FPLFTKKIFINTTQRDYNKADAVVKFLESIPIEMEYKKGLNIVIQALNELKDSLKNQV
                     LGVIDNQILFPNDKEDTK"
     gene            complement(7238..7429)
                     /locus_tag="VBD89_000794"
     CDS             complement(7238..7429)
                     /locus_tag="VBD89_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135341.1"
                     /translation="MQELNLTPTQTLILFIVLGLLGLLLSLSKSLIDIDLPEDTQVPK
                     PRQNANYGAYIQSQNHYYN"
     gene            complement(7429..7761)
                     /locus_tag="VBD89_000795"
     CDS             complement(7429..7761)
                     /locus_tag="VBD89_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135342.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKDKAEAYFTRLYNQH
                     IARVIIANVMAEYTTTFRKSATSFEEAWEALDYQRTTEIVFRAVNGLPCSEKDTGELE
                     THLSEVSA"
     gene            complement(7850..8356)
                     /locus_tag="VBD89_000796"
     CDS             complement(7850..8356)
                     /locus_tag="VBD89_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135343.1"
                     /translation="MRTLFQSTQKTERECAFCVLKNQDNMIKKKRNPRVLFRNVAYKL
                     SEIEGKTLTEIASFLGFGNSEACRITLYKWKRKKWLKFDLKNGHYRNVEVLHEVTLEK
                     MANKELKEQGLIYKANIYYEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELF
                     AELYTNMN"
     gene            complement(8442..8618)
                     /locus_tag="VBD89_000797"
     CDS             complement(8442..8618)
                     /locus_tag="VBD89_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEO7135344.1"
                     /translation="MTATVRRGRPTESKKDYMLRVRLDDETKSKLEQIEKKTSLSKSE
                     IIRNGIDEQFDRLK"
     gene            complement(8843..9016)
                     /locus_tag="VBD89_000798"
     CDS             complement(8843..9016)
                     /locus_tag="VBD89_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEO7135345.1"
                     /translation="MTDKRAVGRPPKGEQPNNIKITVRISQGLDEKVQAYADNHGLTK
                     PEAIRKALEDLSD"
     gene            complement(9115..9753)
                     /locus_tag="VBD89_000799"
     CDS             complement(9115..9753)
                     /locus_tag="VBD89_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEO7135346.1"
                     /translation="MNIIKQVKSSFGELEIDFYLDRNRNIFVTIEQLAQGFGYKSRNA
                     IEKMIERQPYLKEKRFSVTDKLSATDGKQYETRLFNKRGIFEIGMLSKTEKGKIFRQW
                     IYDHIEELERENANFKLIRELEKSNHKELTQAIKDWEHFNQWSYKAISDLLLKSVTGQ
                     TAKQLKQSRVGYEIALDCLSAEELSKYRELEHKVIVLLELNAEYNDIKKLVL"
     gene            complement(9977..10219)
                     /locus_tag="VBD89_000800"
     CDS             complement(9977..10219)
                     /locus_tag="VBD89_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HEO7135347.1"
                     /translation="MKATTYKELKKWIDEGVDLAELAQGYADKVPNADREQFEAITQE
                     IFNVLEGVSLMLDDKVLIYNRKAEQKRLNDIEQGNY"
     gene            complement(10246..10866)
                     /locus_tag="VBD89_000801"
     CDS             complement(10246..10866)
                     /locus_tag="VBD89_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEO7135348.1"
                     /translation="MRTETWNGYTIRFVEHQGEWWAVLADIAKALDLNPKFIKQRLGD
                     EVVSNNHVADSLGRQQEMLIVNEFGIYETIFSSRKKEAKTFKLWVFEIIKQLRQSTGL
                     EGFQVFRMFDKEHQKKAMNRLVDGLQNATKKDLIKANTIANKAVSDLYGYPKMVSKNE
                     MTENMLRDREPILDETVELIKVKEKYGLNFSVSEAIYNQNTIKKAQ"
     gene            complement(10890..11090)
                     /locus_tag="VBD89_000802"
     CDS             complement(10890..11090)
                     /locus_tag="VBD89_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7135349.1"
                     /translation="MDKLRGYRVMLGLTQKDMSDKLNISLQSYNNKETGKSAFNDKER
                     LAIKSMVSEIKPDITIDELFYS"
     gene            11296..11910
                     /locus_tag="VBD89_000803"
     CDS             11296..11910
                     /locus_tag="VBD89_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135350.1"
                     /translation="MNRLKELRKEKKLTQEELAGEIGVSKITILRWENGERQIKPDKA
                     KELAKYFNVSVGYLLGYAPNKKIDFQLNLDGTTLHLTKEQFLALENTSKSIKKIKNTI
                     NESVKQEEYIKNASKYYDFEKVSRRLTDRLFEIHTDLIELLMMLDHFPSGELSKSQQE
                     AIFKFYKQLDYFVTDTPASFDYFKKNLESYGYKIYTEGDKIDFD"
     gene            11922..12209
                     /locus_tag="VBD89_000804"
     CDS             11922..12209
                     /locus_tag="VBD89_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEO7135351.1"
                     /translation="MLRRVNHVKNVLAHGEFAEWIENKIGIHYREANRMMTVAKQIPN
                     VSTLKYLGATAKHVNGVAKRKQNFLSQISLIPTNPQLPHQTIINTYLYWQP"
     gene            12925..14070
                     /locus_tag="VBD89_000805"
     CDS             12925..14070
                     /locus_tag="VBD89_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7135352.1"
                     /translation="MKIKEHKKKNGAIVYRASIYLGIDQVTGKKAKTSVTGRTRKEVK
                     QKARHAQDEFISNGYTVTKVVPIKNYQELAELWLENYQLTVKPQTFIATKRMLYNHLI
                     PIFGTMKVDKLTVSYIQRFINDLSNQLVHYGVVHSINRRVLQYGVSLQLLPFNPARDV
                     MLPKVPKKENKAIKFIAPEDLKALMAYMEKLANKKFSYFFDYVLYSVLLATGCRFGEV
                     VALEWSDIDLENGTISITKNYSRLLKLIGTPKSKAGVRVISIDKKTINLLRLYKNRQR
                     QLFIETGTRVSAVVFSTPLKEYQNMATRQESLDRRITEVGIPRFTFHAFRHTHASLLL
                     NAGISYKELQYRLGHATLAMTMDIYGHLSMDKEKEAVSYFEKAINNL"
     gene            14166..14402
                     /locus_tag="VBD89_000806"
     CDS             14166..14402
                     /locus_tag="VBD89_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO7135353.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGDAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            14395..15093
                     /locus_tag="VBD89_000807"
     CDS             14395..15093
                     /locus_tag="VBD89_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO7135354.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            15090..15335
                     /locus_tag="VBD89_000808"
                     /pseudo
     CDS             15090..15335
                     /locus_tag="VBD89_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            15332..15784
                     /locus_tag="VBD89_000809"
     CDS             15332..15784
                     /locus_tag="VBD89_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO7135355.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEGLPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRKAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            15781..16737
                     /locus_tag="VBD89_000810"
     CDS             15781..16737
                     /locus_tag="VBD89_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7135356.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            16826..17203
                     /locus_tag="VBD89_000811"
                     /pseudo
     CDS             16826..17203
                     /locus_tag="VBD89_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            17216..17629
                     /locus_tag="VBD89_000812"
     CDS             17216..17629
                     /locus_tag="VBD89_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7135357.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWNPAYANLVDQFGVMWQISTELN"
     gene            17873..19252
                     /gene="glmU"
                     /locus_tag="VBD89_000813"
     CDS             17873..19252
                     /gene="glmU"
                     /locus_tag="VBD89_000813"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO7135358.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            19273..19827
                     /locus_tag="VBD89_000814"
     CDS             19273..19827
                     /locus_tag="VBD89_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7135359.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            19827..20096
                     /locus_tag="VBD89_000815"
     CDS             19827..20096
                     /locus_tag="VBD89_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135360.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            20106..20795
                     /locus_tag="VBD89_000816"
     CDS             20106..20795
                     /locus_tag="VBD89_000816"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO7135361.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(20835..21482)
                     /locus_tag="VBD89_000817"
     CDS             complement(20835..21482)
                     /locus_tag="VBD89_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO7135362.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            21649..22575
                     /locus_tag="VBD89_000818"
     CDS             21649..22575
                     /locus_tag="VBD89_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO7135363.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            22746..23462
                     /locus_tag="VBD89_000819"
     CDS             22746..23462
                     /locus_tag="VBD89_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7135364.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            23464..24297
                     /locus_tag="VBD89_000820"
     CDS             23464..24297
                     /locus_tag="VBD89_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7135365.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(24349..25152)
                     /locus_tag="VBD89_000821"
     CDS             complement(24349..25152)
                     /locus_tag="VBD89_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO7135366.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            25318..27768
                     /locus_tag="VBD89_000822"
     CDS             25318..27768
                     /locus_tag="VBD89_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7135367.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            27850..29565
                     /gene="pabB"
                     /locus_tag="VBD89_000823"
     CDS             27850..29565
                     /gene="pabB"
                     /locus_tag="VBD89_000823"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO7135368.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
BASE COUNT         9141 a         4905 c         5624 g         9910 t
ORIGIN      
        1 gacgaaatat tatttactaa taatggatga ccagaaacta ttttatccta tgaaaacaat
       61 agtagccttt agatgaacta aaaggcgaag aaactttaaa ttatgatttt aaataagcaa
      121 taaaccttga tatatcaatg gttaaagaca ataaaggaga acaatatgac atttgaagaa
      181 attttgagga ttggctattt ttatatgtct ttaaatcacc ttaactattg atataacaac
      241 gtttctaggg atttcctata actttaatta tccttagata ttctttcaaa gtccacaaaa
      301 aagtccacaa atttgggcat aaaaaaagac tatcaactca atggttgata gcctaaagaa
      361 gtagtataag gtgaacaaat ttttaacgag ttcagcaggc aagaactagc gtagtttaaa
      421 tactacgctt ttttcattat ctaatcaaaa taatattggt attttttcat aaaaatttag
      481 aagtgctatt tctcgtaaca ttatatattc tgaaaggctt atctgattat cagtacaaaa
      541 ttgtctcctg cttatcctat ttttgctagg ttttaagtat tgattggtca agatagctta
      601 tatagacgtt taaatacagc atagttttta aatattctat ccgcttgttt aatttctttt
      661 atcccttcaa agtcagtttc gataaactac agtgacaaaa tattgaaaac caccagcaag
      721 tccatttgtt tgtgtgttaa cagagataac ttctttttga ttttccatga attgattgat
      781 ttctttatca aacttttcta tcaattcatt aatgggtgtt ggtttaaacg acttatctgg
      841 atatgccaca tataagatat aaaggtacta tgatttatta atttatttta tttagaattc
      901 catccctata atgctccgca aactcaagca aagctctttt cttagtctcg tagtaccaac
      961 tttcagagcg attaagtttc gtcatgatct cttgattaga taacttttca ctaatcaagt
     1021 aacactcgat cagtatttgt ctgtagtcta tcttagacag ttggttaatg gcatacttaa
     1081 ttgcacctag ttcctctagg gcgcattctc ggcttatttc aaggtacgtt ctgcgcgtgg
     1141 aatgatattc tatatcaaac tggtaaagct cgttataggt caaatcaagg ctattggcga
     1201 tacgttgcca cctctggaac tccttaagtt tacgcatggc gttcttcttg ctcatctaac
     1261 acctctaaag cttctctatg tagtttgaaa acagtattcc ttgaacaacc tactttatct
     1321 ggtatctcgt cccatgataa gtcatccaca tatctagcct taataacagc tatctgattc
     1381 tcatcttgta gggaggtaat catggctagc ctcttgtcac gttcttttgc caaacataag
     1441 agttgctgtg cagtaccttt ttcaatgcca tttagtaact caggattacg aaaagcattc
     1501 atcagcttga tgtccttgtc tcgttgttcc tcaaataagg tcattaaagc aaaaagtggc
     1561 ttcaattctt ttaattgttc tttagcgccc atgattaact cctttttggt ataatttagt
     1621 taagcttaaa tttaaccaag gagacgttct gtgtgagcgt ctttttgtgt ttctcgtttt
     1681 gtaaactaga tattttttgt agtacttcgc ttttggcttc taaaatcgtt tctaagcgct
     1741 ttttaaagtt ggtaatataa aatatcaact tgtcagatag aagttctaaa accatgtttt
     1801 attttagtct gataaagtca ttctttgtgg tagggtactt agcttaaatt agatataaaa
     1861 tcctttagaa tcaagctttt aatttttatc aaaaatttca aaaagggaat tttttgcacg
     1921 gaaaagggcg gcgttcttaa gtttccgaac aatgtagccc cgttcaaaaa caagtggggt
     1981 atttcccgaa tgttatagcc agtgcctgtc tcccttatcc atatagtaat ggaatctctt
     2041 ccaatgatac aagtatttag ctattctcat gtattttgga cgcttaggaa agtcatcatg
     2101 actataataa ccatgtttgt gtttggcttt tggatctact ttcaaacact ctttaaaggc
     2161 taatcgccag tagtagcaac agtctgtctt gcttcgattg agtgtcgctt gatgaacttt
     2221 ctgacaagaa ccacaagcaa aatcatgaga agccttaaat aacttccggc aacgtctctc
     2281 acatttagga cacaagaaga agtagcgttt accaccataa gttcctggta tcgtttcaag
     2341 taataagttt tgatggttat aatgaataac cagattatct aggtctatgc gaataggttg
     2401 gttatccatt gttcctgtaa tctttgtctt cctctgtgcc ttcattggtt taatgatact
     2461 ttcaatagct agttctaaca ttctttctcc ttaaaactca aacaacccaa aactattgat
     2521 aaaacgacaa aaagagggaa atccctctga ttgtttattt aacgagtaac tgaccttcaa
     2581 caaccatatc atacaaatgg ttaaaggctt gactgatagt ttcaaggata gctcctaaat
     2641 cgtcttggct catctcctga taattcatag agagatgttc agctagttgg tcatggtctg
     2701 agatgaaagc catgagtgtg tcttgttcat tagcttcctc ttgagtaatc tcaaataaag
     2761 gaaccacgcg ctgatcatca agttcttcaa cttccttaat taattcattt tcctgagcca
     2821 tgtcatcaag ttcttcatcc gtcatatcct cagattggtt gtaatagtcc ttgaaactgt
     2881 cacagatacg cttgaagacc ttgcttagtt tcttatcttc cacatactcc agcactaact
     2941 gacccttgcc tttaatcgtt accccaatca cgggcgcgtg atatgtccca gtcatatacc
     3001 ctagaatggc atggcaagct acctgagcgg tgtcaaagtc tttaaaagtg tagtggaatg
     3061 tgaatgtttt tggtgtgtct gaaaatgttc tcatgttatt tctcctttgt gattgctata
     3121 atgtctgata aattgataat ggcagaagga cacgctaccc agtttggttg ttggccagat
     3181 aaaagatatt tgaccaactc attataaagg gtgcggtctc cttgtatggt gatggtgtta
     3241 ccacctcgtg tgtgtaattg tagtttcata tcagttacct gtacaagacc aataaagaag
     3301 ttgtggtact catgtcttca tagttgccat aagttgcttc ttgatattta atatcaatca
     3361 ctttcttatc agcaataaaa gaattcaccc tgttttcaaa ttcctcatta gtttcatttc
     3421 tgaattttgg caaatccata tactcgttaa agcctgtttc aattcgcttg cgattaaata
     3481 gtttaatttt catttttctt tatcctttct gattttgtga catttgtgac attgtgtgac
     3541 gtttttaaaa aactcgtaat cccttggggg gcaatggttt gttacatttt ttacttttat
     3601 gacatagggg ttaaactcta taactagata taagagtaaa gcactcttct ttctctttta
     3661 tagcaattat atctaatgta ataaatgtat aaaatgtaac aaagccttgg ggctgtaagg
     3721 gtagctcgtg tgacgttttc taattgtaat gtcacaaatg tcacaaacta cttgtgtttt
     3781 gctttgctat tccaataccc tctatcttgt tttggtctct tgcgtttctc tggtgtctct
     3841 ctaccgtttg aataggtcgc aagactagca taatcgggtg tatcctcttt agggtaaaaa
     3901 cctaaatgta attgcctacc tgatgggata gttcttacac cagttctaat gtaaggtggt
     3961 aaaacctgct taatatctct gtgcaatcct ctttcggtct taaaaaacga ttgattaaaa
     4021 taatcttgat actgtttcca gcaataccaa ataaaatcat tgggaataaa ttcactcgct
     4081 aaatcttctc taaacgtctt ttcgacaaaa tccaaaacag ggttaatgtc cttttggtgt
     4141 tcacctaata aggtttgaga ttgcttagga ttgatatcct ctgatggtgt ttcaattgct
     4201 aacttaacca aatactctag gacttctttg cgattgatat aatctttctt gatagctggg
     4261 tttggtttat ctttaaatct gcttgaaaat atcaagattc taaaccgcct gtcaattgct
     4321 gatacatcac cattcatacg tgggagacca ttggaagatt gtacaacggt catatttaac
     4381 ctcaacgaat aaggtttttt ccctttgtcc tcaattgtca taatatcacc agtcgcaagg
     4441 ctgaacatgt ccgaggtatc tttgataata gcgtcttttt gaacgtcatc accgataact
     4501 aacgatttcc caagcaagat agaagtggta aaacggcttt tatttaagtc tgtaatttta
     4561 agacttgcaa cgttttctaa accaactaaa tttatcagta actgctgaaa agttccttta
     4621 cctgttccgc cctctccata taaccaaaaa attttctcga gcgattgacc tgtgatactc
     4681 gcttttataa tttgaatggc aagctggtat aactccttat caccgtcaaa cagttctttt
     4741 aaccattctg tcactttcca cccatttata gtaggctctt ttgcataagg attgtaggcg
     4801 gttttgattt ttctagtgac aatgatttta ggattgaaag gttcaaactc gcctgttttg
     4861 ttgttaaaaa gttgtttgcc aataaccgta tactttcctt gaatttcttt catttggctt
     4921 tgtctggcaa ttttataaag ggtgtcaaaa gcctgttttt ccgttgcgtt tgggaaaatt
     4981 gtagaaataa ggtcttgtaa aagttcgtta tcctctaacc agataccaaa atcagggtta
     5041 tagtagtaca gaggggcttt ctgtccttgc gcttctggtt taatcctgat aaagtggata
     5101 tattgtttca gcatgatagc aacccctagc ggtgttttag gcaacgcttt ttcgcttgct
     5161 tcttgtcctt tttggtgggc tagttcttta tgctgtacct cggtcaatct gcccgctttt
     5221 acattctcaa gatgttcgct gtctgccatg acctcatcat aagctatctg ataggcttct
     5281 tctttaatac tctgacattc cttaataagt tgccccctaa cgcttttgaa agtcttgaaa
     5341 tacttatctt cactttcacg cgcctctaag atttcacttt caaggctttt caaatcttct
     5401 ttttctatgg ctctatcctc tctttctaaa ttctgctctt gctatactgg taaaagtgcg
     5461 gtctaactcc tcaatcggta atgggttatc tgtcacaccg ttagctatct ttgtcaactc
     5521 gtaagctgtt tctatatcac aatcaaccca tttattaaat agcaaaccaa caaatttagt
     5581 taaggctaca ttacgtccgc cttcgtctcc aaaaccatta aacaaggtat ctatgaccct
     5641 catggtaata gaacgctgac ttctagggcg tgacgtgtaa gtagtagtaa cctgtcggtt
     5701 gggtgcttta gggacaggat aatcaagacc acggttcaca tagcgctgat agtcttctgg
     5761 gtcgcctgtt gtgacgggta agccttgtaa ttgcgaccag gtaagactag ctaaatcaaa
     5821 cggtagtcca atcttattag ctatctcctt gaccacttgt ttatacgtcg ttttgtccat
     5881 agtgtcacta ggcttcacga caaggcgata gcgtggcttc tcaggggtgt gtttaatcgt
     5941 tggataaata atatagctgt attcccaaag cgtcttagaa acgattttag gtaggttagc
     6001 gcctgtttct atctcgtcat agtcaagaaa aatcaaatcg cgataaacta aactagcatt
     6061 attgcgctta tagccaccgt ttttctctgc tgtgactttg ccactcaggc agtaaggggc
     6121 ttgcgttcgc ttgtattctt caatattgat tccttcagga ggtttcaaag gtttaaactg
     6181 agcaatatag tcaaatggtt ctaattgtcc tttatagggg tacaaataag agctaaagcc
     6241 tcttgcttca taaatagcca tctacacatt taccccccaa aaaataagaa tatcactgac
     6301 tttatagtaa tgcttcctcg tatcttctag tggtggttgg tatcgtctta acccagcatt
     6361 ttcccaccgt tttagagttt tgcctttgat atttaattcc tctttgacct gttcagccgt
     6421 tatcaaccct aaaactcttg gtttaggttt ctggtaggct tccaaaaagc gattaaaagc
     6481 ggtcaggttt tgttctaaga gtttggcttc ataatcttga ctaaataagc tcatgcctaa
     6541 cctcctttga gtaattcctt ataactggtt aaatcggcat tcaataacac acttaggcgt
     6601 tcctgttcct tttgtacttg attataaaag gctttagcac catctagtaa ttcttctttg
     6661 ttagctggga taaagtaccc acgattgaat ccgtgtctaa tgccgataat agggatgtta
     6721 tagcgtgtga ttaagctact gatgatactt tggacggaac gctcttcaag tttcagtatt
     6781 aagctaatct ctgcccctgt aatggggttg tctgctccaa ccttgatcag attaaggaca
     6841 cgtctataat tctctggtag tgtcatttcg tgtcctcctt atcatttgga aacaggattt
     6901 ggttatcaat aacaccaagc acttgatttt taagactatc ttttaactcg ttcaatgctt
     6961 gtataactat atttagccct tttttatatt ccatctctat tggtattgat tccaaaaatt
     7021 taacaacggc atcagctttg ttatagtcac gttgtgtagt attgataaat atttttttcg
     7081 tgaataacgg aaaatcgtcc cactctgctt caatatcttc atctagcatt ttgatatttt
     7141 tgtctaaaac cctatcactc aaccagtttg taggctgttt ttcgctatct tctagcaatt
     7201 ttttaaaata ttcttccgtt gtcatgcagt tccctcccta attgtaataa tggttctgtg
     7261 attgaatata agccccgtag tttgcgttct gacgtggttt agggacttgg gtatcttctg
     7321 gtaaatcaat gtctattaat gacttagaaa ggctaaggag aagccctaag agacctaaaa
     7381 caatgaataa aataagtgtc tgtgttggtg tgaggttaag ttcttgcatc atgccgatac
     7441 ctcacttaaa tgagtttcta attcccctgt gtctttctct gaacaaggta aaccgttaac
     7501 ggctctaaag acaatctctg tagttcgttg atagtctaaa gcttcccatg cttcttcaaa
     7561 gctggtggca cttttcctga atgtagtcgt atactctgcc attacattag caataatcac
     7621 ccgagcaata tgttggttat atagtcgagt gaaatatgct tcagctttat ctttgctgag
     7681 ttggcgattt ttgaacattt ctagctgttc aggagtgtat ctatcttttg aaaaaggatt
     7741 tgtttctact ctatatttca ttatgtcttt tctcgcttaa ttttattttc tgtgtaattg
     7801 ccggtttcct atactagatt catgctagtt ttaaggggta gctccctgat tagttcatgt
     7861 tagtgtataa ttctgcaaat aactcgctag gaatacgctc tagcgctttt tgttgtaagt
     7921 ggatggcttt aattctatct tgtgttttgg ttttaatgtc ttctataatt tcggatgttg
     7981 agaccacttg ttcatagtaa atgttagctt tataaatgag tccttgctct tttaactcct
     8041 tgttagccat tttttcaagc gtaacttcat gtaatacttc aacattacga taatgaccgt
     8101 ttttgaggtc gaattttagc cattttttac gcttccattt atatagagtg attctgcaag
     8161 cttctgaatt cccaaaacca agaaaagaag cgatttctgt taatgttttc ccttcaattt
     8221 cagatagttt ataagcgacg tttctaaata acactctcgg atttcttttt ttcttaatca
     8281 tattgtcttg atttttgagc acacaaaaag cgcactccct ttctgttttt tgtgttgact
     8341 gaaaaagagt acgcatgtat aatatttacg tacctgtttt caggtcggaa gctcctagcg
     8401 tgtgcggtcg ccaaactaca acacgttagg ggcttgtttt tctatttgag acggtcaaac
     8461 tgttcatcta ttccgtttct gattatctca gactttgaaa gagatgtttt cttttcgatt
     8521 tgctctagtt tagacttggt ttcatcatca agcctaaccc tcagcatata atcttttttg
     8581 ctttcagttg gtcgtcctct tcttacagta gctgtcatag agttttatca ctcctttctg
     8641 tcgctacaaa ataaattata ttatgtagct acataatttg tcaaccccta aattaaaaaa
     8701 acatgcgtat tctattttca atgtgcaaat ggttcacaaa atagagtacg catgatatac
     8761 tatttacgta ctcactttgt gggtcgagcg atagcatagt aaccaaaaat tggcgttggc
     8821 ggttactgtg ctattttttt gtttagtctg acaaatcttc aagggctttt ctgattgctt
     8881 ctggctttgt taagccatga ttatcagcat atgcttgtac cttttcatca agtccttggc
     8941 taattctaac cgttatcttg atattgttcg gttgctcgcc ctttggtggt ctgccgactg
     9001 ctcttttgtc agtcattgta tacctcctta gttttgacga cataattatt ataattaagc
     9061 cgtcttaata agtcaacccc taaatcaaac ttttttgcgt actctattca gttgttaaag
     9121 gactaatttt ttaatatcgt tatattctgc atttagctct aacaggacaa tcaccttgtg
     9181 ctcaagttct ctatattttg ataactcctc cgcactcaaa caatctaatg caatttcata
     9241 acctacccgt gactgtttca gttgtttagc agtctgtcct gtgacagatt ttagcaaaag
     9301 gtcgctaata gccttgtagc tccattgatt aaagtgttcc caatccttga tagcttgtgt
     9361 taattcttta tgattagact tttcaagctc tcgtatcagt ttaaagttag cgttttctct
     9421 ttctagttct tcgatatggt cataaatcca ttgacgaaag attttacctt tctccgtttt
     9481 ggacagcata ccaatttcaa aaatacctct tttattgaat agtcgggtct cgtattgttt
     9541 gccatcagta gctgacaatt tgtcagtaac tgaaaatcgc ttttctttga ggtagggttg
     9601 gcgctctatc atcttttcaa tagcatttcg gctcttatat ccaaatccct gcgctaattg
     9661 ttcaatcgtc acaaaaatat ttctattcct gtccagataa aaatcaattt caagttctcc
     9721 aaaagaactt tttacttgtt ttatgatatt cactgtttta cctcgttatt tgattattgt
     9781 tgttttgtcg gggagaactg caactgttaa gtcgttgcct gctctgcggt agtatgatac
     9841 aatggaagta tcaaatcttt tactaaaacc cctttaataa tagcttgcct gctttattat
     9901 ttgagtttag ttatactagt taaaggctgt gctgattggt ctcggtaagc ctttttttgt
     9961 tgctcttgat tattgattaa taattgcctt gttcaatgtc attcaaacgc ttttgctctg
    10021 ctttgcgatt atagattagc actttgtcat caagcatgag cgatacgcct tccaatacgt
    10081 tgaaaatttc ctgtgtgatt gcttcaaact gttcgcgatc tgcatttggt actttgtcag
    10141 cgtaaccctg tgctagctca gctaaatcaa caccttcatc aatccatttc ttcaactctt
    10201 tgtaagttgt tgctttcata atcatttctc ctttatccac gcgcatcact gcgctttttt
    10261 tattgtgttt tggttataga tagcttcaga tacgctaaaa tttaagccgt atttttcttt
    10321 aaccttgatt agttcaaccg tttcatcaag gataggctca cggtctcgca acatgttttc
    10381 tgtcatctcg tttttactaa ccatcttagg ataaccatat aggtcagaaa cggctttgtt
    10441 tgcgatagtg tttgctttga taaggtcttt cttagttgca ttttgtaagc catcgacaag
    10501 cctattcata gctttctttt gatgttcttt atcaaacatt ctaaatactt ggaagccctc
    10561 tagccctgtg ctttggcgta gttgtttgat aatctcaaat acccataatt taaaggtttt
    10621 ggcttccttc ttacggcttg agaagatagt ttcataaatg ccaaactcat taacgattaa
    10681 catttcttgt tgacgcccta aactgtctgc gacgtggttg tttgaaacaa cctcatctcc
    10741 caaacgttgt ttaataaatt ttggattcag atctagtgct ttagcaatat cagctagcac
    10801 cgcccaccat tcaccttggt gctctacaaa tcggatagta tatccgttcc atgtttctgt
    10861 tctcaataag ttgtcctttc tttaatcttc tagctataaa ataattcatc aatggttata
    10921 tctggtttga tttctgaaac cattgactta attgctagtc gttctttgtc attaaaagcg
    10981 ctcttacccg tctctttatt gttgtaagac tgtaaagaaa tattcagctt gtccgacatg
    11041 tctttctggg ttaaccctag catgacacga tagcctcgta gtttgtccat gatgtatcct
    11101 ccttttgaaa aaattcctcc cgtaggttga aagtgagaaa ggttgcggga gataaaagta
    11161 tccttttcgg ataacttcaa aacgattata tatctgtttc ggttacttgt caagaattat
    11221 tttgaaattt ttatatcatt tcttgatact ttttataagt tcggttataa tcaactttga
    11281 aaggtagtga aataaatgaa taggttgaaa gaattacgca aagaaaaaaa gctaacccaa
    11341 gaagaattag ctggggagat tggtgtatca aaaatcacta ttctccgttg ggaaaacggc
    11401 gaaagacaaa taaagcctga caaagcaaaa gaattagcaa aatattttaa tgtatcagtt
    11461 ggatatttac taggttatgc tcctaataaa aagattgatt ttcagctaaa tttagatggt
    11521 acaaccctcc atctaaccaa agaacagttt ttggctttag aaaacacctc gaaaagcata
    11581 aaaaaaataa aaaataccat caatgaatct gttaaacaag aagaatatat aaaaaatgct
    11641 tcaaaatatt atgattttga aaaagtcagc agaagactta cggacagact ttttgaaata
    11701 catactgacc ttattgagct attgatgatg ttagaccact tcccaagtgg tgaactttcc
    11761 aaaagtcaac aagaagctat tttcaaattt tataaacaat tagattattt tgtgactgat
    11821 acccccgcta gttttgatta ttttaaaaaa aatttggagt cctacggata taaaatctat
    11881 actgagggag ataagataga ttttgattaa ataaataata aatgctcaga cgagtaaatc
    11941 atgtaaaaaa tgttttagca catggagagt ttgctgaatg gatagaaaat aaaattggta
    12001 tccattacag ggaggctaac aggatgatga cggtagctaa acaaattcca aatgtttcaa
    12061 cgttgaaata tttaggggct acagcaaaac atgtcaacgg agttgcaaaa agaaagcaaa
    12121 attttctctc ccaaatctcc ctgataccga ccaaccctca actaccccac caaacaataa
    12181 ttaacactta tttatattgg caaccatagc attccttagc attaacatgt caaatacgac
    12241 cacatggtcg catttaggtt caagagcctt ccgccatagg aagcaatagg ttattcaagc
    12301 tgttcttact tttggttatc tggtagggtg gcaataaggt agagagcttc tgttccgata
    12361 tttcccaacg tcgggaaatt tggtaattca tgagctattt tcatcattca ctttgcaaaa
    12421 gtataatcga tttccaactt atgcaactac tcctccaata aaagcaatgg cagttagaaa
    12481 cgtttgtctc tttaatgatg tgataattta gtattcttat cttcggggtg gggggtcgtg
    12541 tgagaaaaaa agcaaatatt tggacagttg acccttccca ccggtttcaa aacttggatt
    12601 gaacaacatt tttttatgat gggggtaacc ttaaaggaat agccatgaat gaattacaat
    12661 ttttgatata tacggctaac aatagccaag agacagccag cttttctggg ctatctaaaa
    12721 atttaccaac ttactaaaac caaacaaaaa aagacctcgc aagttttcca cgctcgcaag
    12781 gtcttaaaaa acaacaatat tatatcacaa ttttctattt ataatatttc ggatattaac
    12841 ccgataccgt tattatacca tgatataaac taatctaaaa cccttttaat aatagcttgc
    12901 ctgctgatgg aaaggtttat catcatgaaa ataaaagaac ataagaagaa aaacggtgca
    12961 atcgtttatc gtgctagtat ttatctaggc attgaccaag taacgggtaa gaaagctaaa
    13021 actagcgtaa caggtaggac acgaaaagaa gttaagcaga aagcaaggca cgcgcaagat
    13081 gaattcattt ctaatggcta cacggttact aaagttgtcc caataaagaa ttatcaagaa
    13141 ttggctgagt tatggctaga aaattatcag cttacggtaa agcctcagac atttatagca
    13201 actaagagaa tgctctataa tcacttaata cctatttttg gaactatgaa agttgataaa
    13261 ttaactgtca gctatattca gcgcttcata aacgacttat caaatcagtt agtccattac
    13321 ggtgtagttc attcgataaa tagacgtgtt ttacagtatg gcgtatctct ccagttattg
    13381 ccattcaatc ctgcgcgtga tgttatgttg cctaaagtac caaaaaagga aaataaggct
    13441 attaagttca tagctccaga agatttaaaa gcgttaatgg cttacatgga aaagctagcc
    13501 aataagaaat ttagctattt ctttgattat gttctataca gcgttttact tgctactggt
    13561 tgccgatttg gggaagtagt agccctagaa tggtctgata tcgatctaga gaatggaact
    13621 atcagcataa ccaagaatta tagtaggtta ttaaagttaa ttggtacgcc aaaaagcaaa
    13681 gcaggggtaa gggtcataag catagacaag aaaacaatca atctgctacg actttacaag
    13741 aataggcaac gacaattatt tatagagact gggacgcgtg tttctgctgt ggtattttca
    13801 acaccactaa aggaatatca aaacatggct actagacagg aaagcctaga taggcgcatt
    13861 actgaagttg gtatccctag atttaccttt cacgctttcc gacacactca cgctagttta
    13921 ttgctaaatg ctggtattag ttataaagaa ctacaataca gattaggtca tgccactttg
    13981 gctatgacca tggacattta cggacacctt tccatggaca aagaaaagga agctgtttcc
    14041 tattttgaga aagctataaa taacctgtaa gtccacaaaa aggtgaacaa atttattttt
    14101 aggagtttat aggctcattg aaaagcctat taaatcaacg tttatagaca ataaaggaga
    14161 acaatatgac atttgaagaa attttaccag gtttaaaagc aaagaaaaaa tatgtacgta
    14221 caggttgggg ggacgctgaa aattatgttc agttatttga tacccttgaa gttaatggga
    14281 aagtactaca ggctacacct tattttctta ttaatgttac tggtgaaggt gaaggttttt
    14341 ccatgtgggc accaacacca tgtgatgttt tagcagagga ttggatcgag gtcaatgact
    14401 aaagttgttt tagtgacggg ctgtgctagt ggaattggtt atgcacaggc tcaatatttt
    14461 ttgaaacaag ggtatcaagt atatggtgtg gataagtcgg ataaaccgaa cttaaatggt
    14521 aattttaact ttataaagtt ggatttatcc tctgatttat ccccactatt cacaatggta
    14581 ccgactgttg atattttgtg taatactgca ggtattctag atgcctacaa gccacttcta
    14641 gaagtaagtg atgaagaact ggaacaccta tttgatatta attttttcgt gactgttagg
    14701 ctaacacgtc attatcttag acgtatggtg gaaaagaagt caggtattat tatcaatatg
    14761 tgctcaatag caagttttat agcgggtggc ggtggagctg catatactag ttctaaacat
    14821 gcccttgcgg gattcacacg tcaattggct ttggattacg caaaagattg tattcaaatt
    14881 tttggtatag cgccaggtgc tgtacaaact gctatgactg ctagtgattt tgaacctggt
    14941 ggattagcag aatgggtagc tagtgagacg ccaattggtc gttggactaa accaagtgaa
    15001 gttgctgaat taactggatt tcttgcttca ggaaaagcga gatcaatgca aggtgaaatt
    15061 gtcaaaattg atggtggctg gagtttgaaa tgagaaaata gattgaattt ggttgtggta
    15121 atacttggtt attacgtacg gaatttgaag catctgatgg tactgagcgg gaagttaaag
    15181 gaatatcagg tgatatttta ataaaatctg tttatttacg tgtttggatt ggtcaaactg
    15241 tttatatttt agatgttaaa gaaggattta aaagacaaag aaaaatgagt aacgcagtta
    15301 agtttgttgt gggaattgct tcggaggtaa tatgaaggca caagaattat ggaataagtt
    15361 taaatctttg aatccttcaa tcggtgatga gatagacgca tggcaatttg gagcagaacc
    15421 agatcttctg gcaaagttag ttgtagatgg gacaaaaact gcaacggcct ctgcctatga
    15481 tttatatgaa ttagatgagg aagggttacc acaagttggt tcatatgata ttattttgga
    15541 tagcaatgat gaagctatct gtattattga aataactaaa gttgatattg tcccttttaa
    15601 agatgtctct gcagaccatg cttttaaaga aggagaagga gataaaactt tagaatggtg
    15661 gagaaaagct cacattgatt tctttaaacc ttattttgag gagtttggtt taatgtttag
    15721 tgaggactct aggatagttt tagaggaatt ccaagtggtt tacccaaagg agattaacga
    15781 atgaaattag cggttttggg gacaggtatg attgttaagg aagttttgcc tgtcttgcag
    15841 aaaattgaag gtattgatct agttgctatc ttatcaacag ttagaagttt ggagacagct
    15901 aaagatctag ctaaggaata taacatgtct ctagctacga gtgaatataa ggctgtcttg
    15961 gacaatgagg agattgacac agtctatatt ggactgccga atcacttgca ttttgactat
    16021 gctaaggagg cactcttggc aggaaaacat gtcatttgtg agaagccatt tacattagaa
    16081 gcaagtcaat tagaagaact tgtatctatt gccaatacta ggcaactaat attactcgaa
    16141 gcaattacaa atcaatattt acctaatttt gaccttgtaa aagaacattt aagcaacctt
    16201 ggagatatta aaattgtaga atgtaattat tcccaatatt catcgcgtta tgatgccttt
    16261 aaacgtgggg aaatagcacc agctttcaat cctgaaatgg gtggaggtgc gttgagagac
    16321 cttaatattt ataatttaca tttagtaatt ggactttttg gagaacctat aacagcacag
    16381 tatttaccaa atatagagcg tggtattgat acttcaggag ttctagtcct agactatggg
    16441 cactttaaaa cagtctgtat tggagcaaaa gattgtagtg cagaagtaaa atctactatt
    16501 caaggtgata aggggtctat agcgatttta gggccaacta atactatgcc taaaatttca
    16561 ttaactatga atggacaaga aagtcatgtt tatcaattaa atggtgatag gcaccgcatg
    16621 cacgatgaat ttgttatttt tgaaggtatt attagcaatt tagattttaa gcgtgctgct
    16681 caagctttag aacatagtag aacggtgatg aaagttctag atatggtaac gaaataagta
    16741 aatgactctt ttgagtcatt ttttacttat tttaaatact gtatgttata ctaattagta
    16801 ataaaaccga aagtgagata ataaaatgat ttcatcaatt ggacaggtaa tgctctacgt
    16861 tagcaatgtt gaagcatcag cagatttttg gaaaaacaag gtgggttttg agcgtgttga
    16921 aaaatagaca caaggagatt atgtaaccta tatagttgca ccgaagttgg atagtgaagt
    16981 tagcttcgtc ttacatgaca aagcaattat agctcaaatg agtcctgagc tagatttagc
    17041 aactccctct attttatttg aaactactga tattgacagt acatatcaag aattaacagc
    17101 aaatgaggtt atgacaaatc ctattgttga tatggggagt atgagagttt ttaatttttc
    17161 cgataatgac aataattact ttgcaatccg tgaagtaaaa taaaaaggag aacttatggt
    17221 taaagcacta gaaacataca tcgtaacaaa tggaaatggg cgacaagcgg ttgattttta
    17281 taaagatgtc tttcaagcgg atttagtgaa tatgatgaca tgggaagaga tggaccccaa
    17341 ctgtcttgaa gaccgcaagg atttaatcat taatgctcag cttatttttg atggcattcg
    17401 tcttcaaatc tcggatgaaa atccagattt cgtgtatcaa gctggtaaaa atgttacagc
    17461 cgctattatt gtgggtagcg tagaggaggc gcgtgaaatt tatgaaaaat taaagaagag
    17521 tgcacaagaa gttcagttag agttacaaga aactttctgg aatcctgctt acgcaaactt
    17581 agtagaccaa tttggtgtga tgtggcaaat ttcaacagag ttaaactaat cgtttttacc
    17641 atctgagcat tgcaatgata gatttataaa aatactgatt gcaaacagag aagaaaaaat
    17701 taacggattt catgtattga atagctacca aaaggtagct attttattcc taattgaagt
    17761 aagctatttt agctaaagtc tattgtaata gggatttaaa ggggtattag tgataagttg
    17821 aaggcattat gatataatag gttgaattat attcagagat agaggaatta atatgtcaaa
    17881 ttatgccatt attttagctg cgggtaaggg aactcgcatg aaatctgatt taccaaaagt
    17941 tatgcataaa gtatctggta ttaccatgct tgagcatgtt ttccgtagtg tgcaagctat
    18001 tgagccaagt aaaattgtta ctgttattgg acataaggcg gagcttgtac gtgatgtttt
    18061 aggtgacaaa tcagagtttg taatgcaaac agaacagcta ggaactggcc atgctgtaat
    18121 gatggcggaa gaagaattag cgacatcaaa aggacataca ctagttattg caggtgatac
    18181 acccctgatt actggagaaa gtttaaaaaa cttaattgat tttcatgtca atcataaaaa
    18241 cgttgcaaca atcttaacag ccgatgctgc aaatccattt ggatatggtc gtattatccg
    18301 taattcagat gatgaagtta ctaaaattgt agaacaaaag gatgctaatg actttgagca
    18361 gcaggttaaa gagattaata ctggaactta tgtttttgat aatcaaagtc tatttgaagc
    18421 acttaaagac attaatacaa ataacgctca aggagagtat tatctaactg atgttattgg
    18481 aattttcaaa gaagcgggta aaaaggtagg tgcctataaa cttcgtgact ttgacgaaag
    18541 tctaggagtt aatgatcgtg tagcacttgc aactgctgaa aaagtgatgc gtcaccgaat
    18601 tgcacgtcaa catatggtta atggggtcac agtggttaat cctgattccg cttatattga
    18661 tattgatgtt gagattggag aagaatctgt aattgagcct aatgtgactt taaaaggaca
    18721 gacgaagatt ggtaaaggta ctttattaac caatggtagt tatttagtag atgctcaagt
    18781 cggaaatgat gttaccatca ctaattcgat ggttgaagaa tccattattt ctgatggagt
    18841 aaccgtaggc ccttatgcgc atattagacc aggaacaagt cttgcaaaag gtgttcatat
    18901 tggtaatttc gttgaagtaa agggttctca aataggtgag aatacgaagg caggtcattt
    18961 aacttatatc ggcaatgcag aggtaggttg tgatgttaac tttggcgcag ggacgattac
    19021 tgtcaattat gatggacaaa ataaatttaa aacagaaatc ggaagtaatg tctttatcgg
    19081 aagtaattct actctgattg caccacttga aattggtgat aatgctttaa cagcagctgg
    19141 atctaccatc acagataatg ttccaattga tagcatagct attggaagag gacgtcaagt
    19201 caataaagaa ggatatgcca ataaaaaacc acatcatcct tcacaaaagt aacacttatt
    19261 tgggaggttc ttatggattt tgaagaaaaa acaattaatc gtcaaactgt ttttgatgga
    19321 caaattatca aagtagctgt tgatgatgta gagttgccaa atggattggg acaatccaaa
    19381 cgtgaacttg tttttcatgg aggagctgtt gctactttgg cggtaactcc tgagcataaa
    19441 attgttttag tgaaacagta tcgtaaggct attgaaggta tttcttacga aattccagca
    19501 gggaaacttg agacgggtga gtcaggttct aaggaagaag ctgcccttcg ggaattagaa
    19561 gaagagactg gctacacagg gaatcttgaa attctttata gtttctacac tgctattggt
    19621 ttttgtaatg agaaaatcgt cctctatcta gctactgatt tacaaaaagt tgaaaatcct
    19681 aggccacaag atgatgatga ggtccttgag cttctagagt taagttatga ggattgtatg
    19741 caaatggtag aaaaaggcat gattcaagat gcgaaaacga tcattgcttt acaatattac
    19801 ggtttaaaaa tgggaggtca ttcttaatgg gaaaaccatt gttaacagat gatatgattg
    19861 aacgttcaaa tcgaggtgaa aaagtttcag gtcaaacaat cttagatcaa gaaactaaaa
    19921 ttatttctac agaagatggc atggagcaac taactgacga gaacggtaaa catatctaca
    19981 agagtcgccg gattgagaat gcaaaacgaa atgaatttca acgtaagttg aatttagttt
    20041 tattcatttt gcttattttg ttggctctct tgttttatgc aatttttaaa ttataaggaa
    20101 atatcatgaa aattggaatt attgcagcca tggaggaaga gttaaaactc cttgtggaaa
    20161 atcttgagga taaatctcaa gagacagtat tatcaaatgt atattacagt ggacgttatg
    20221 gtgaacatga gcttgtttta gtacagtctg gtgttgggaa agtcatgtca gcgatgagtg
    20281 ttgctatttt agtagaaagc tttaaagttg atgctattat taatacagga tcagctggtg
    20341 cagtagcaac aggattgaat gtaggagacg tcgttgtcgc agatacatta gtttatcacg
    20401 atgtagattt aaccgccttt ggttatgact atggacaaat gtcaatgcag ccactttatt
    20461 ttcattcgga taagactttt gtttccactt ttgaagcagt tttatctaaa gaagaaatga
    20521 ctagtaaagt tggtttaatt gcaactgggg atagttttat tgctggccag gaaaagattg
    20581 atgttattaa ggggcatttt ccacaggttt tagctgtcga gatggaaggt gcagcaattg
    20641 cccaagctgc tcaagctaca ggaaagccct ttgtggtggt acgtgctatg agtgatactg
    20701 cagctcatga tgccaatatc acatttgatg aatttatcat tgaagctggt aaacgctctg
    20761 cccaagtatt aatggcattt ttgaaagcat tataaaaaag cccgtgtatc taatgaatac
    20821 acggactttt ttatttatgt agcagataca tatatttgac ttgcgatacg ctcagggatt
    20881 gttagatttt catcgttata ggtgatgtga taagtttttg cataagggtc aatttgtgtc
    20941 aattcaatgg tatcgttgat atgcaaacgg tgttgttcta ggtatttcag tagctgaaaa
    21001 tcatcatgaa cacgactgat aacatatgtg ccaagctgac taatttggtc caatgtcatt
    21061 tggttaattt ctactaaaaa ctctcccttt ttaggaatag tcccaccgtg tggacaaaat
    21121 tgtggaaagt ctaacaactt ttctaattga tcaacaaaag ttgttgatac agtatgttct
    21181 aaaacttccg cttcttcatg aatatcatca gcagtatagt gcaaatggtt aactaaaaat
    21241 acctctacta gacgatgttt acggtaaagg tcagaaacaa ctaataaacc ttgtttggta
    21301 agataatatc ctaaatcttt atctttaaca atcagttgtt cacttatcat ttttttaatc
    21361 atttctgaca cggcaggagc cgagacttgc atcatttcag cgattttctt attggaaatt
    21421 ttgttgtttt tttcacctaa ttcatgaatg cattttaggt aatcttcttt atttggagtc
    21481 atattcagtc tttcttatac ttcttaaaat ctatttttct aagaccatta tatcaaaaaa
    21541 tactgaaaaa gtatttgaca aatactttat aataggttat gctaatatca aaattaagtt
    21601 aacttaattt aatacataac ttatattaat tttaaaagga gcattttgat gaaaaagtgg
    21661 ttagttattg tatcatgttt tgtagcattt ttaggcttag gtgcctgtgc taacaagcag
    21721 gatcaagcca aaaacgaaaa attaaaagta gttgtaacca actctatttt ggcagatata
    21781 acaaaaaata tagccggtaa caaaatcgac ttacatagta ttgtacctgt tggtaaagat
    21841 cctcatgaat atgagccatt accagcagac gttgaaaaaa caactcaagc agaccttgtt
    21901 ttttacaacg gaattaatct tgaaacaggc ggtaatgctt ggtttacaaa attaataaag
    21961 aacgctaaga agaaagaaaa caaagattac tttgcggtta gcgatggagt tgatgttatt
    22021 tatctaaatg gtcaaagcgg aaaaggtaaa gaagacccac atgcttggtt gaaccttgaa
    22081 aatggtatta tttactcaaa aaatattgct aagcaattaa ttgctaaaga tccaaaaaac
    22141 aaagctactt atgagaaaaa cagagatgct tatgtagcta aattagaaaa attggataaa
    22201 gaagcaaaat ctaaatttaa tgccattcca gctaataaaa aattaattgt tactagtgaa
    22261 ggttgcttta aatatttctc aaaagcttat ggagttccat cagcatacat ttgggaaatt
    22321 aacactgaag aagaaggtac accagatcaa attactagct tagttaagaa attaaaacaa
    22381 gtaagacctt cagctttatt cgttgaatca agtgttgaca agcgtcctat gaaatctgtt
    22441 tcaagagaat caggtatccc aatttacgca gaaatcttca cagactcaat tgctaaaaaa
    22501 ggtcaaaagg gtgacagcta ctacgctatg atgaaatgga atcttgataa gattgcagaa
    22561 ggtttagcaa aataagagtg agaatcgaca ttgaaatcat aatgaaatca atgatgtagt
    22621 attctcgctt aaggctgagt tgtaacaatg tcgaaatcct aatctaaaaa ctgacttagc
    22681 tttaataaat acagagaagt ggagtctggt tttataacca gctccattct acttttggag
    22741 gaaaaatgat tattagtaaa cacttaagcg taagttatga taataattta gtgctagaag
    22801 atattaattt acgcttagaa ggctccggaa tcattggtat tttagggcct aatggggcgg
    22861 ggaaatctac gcttatgaaa gctcttcttg gtttggttga ttctactgga gaatcaggga
    22921 ttgggggaga tttgctacct ttaatgggga gagtagcata tgttgaacaa aaaactaata
    22981 ttgattacca attccctata actgttggag aatgcgtttc tttaggatta tataaggaac
    23041 gaggcctctt caagcgacta tcaaaaactg actgggaaaa agtatcccga gtgattgatc
    23101 aagttggttt gagagggttt gaaaatagac caattaatgc cttatcaggt ggacaatttc
    23161 aacgtatgct tatggcaaga tgtctcgttc aagaagcgga ttacattttt ttagatgagc
    23221 cgtttgtagg gattgattcc attagtgaac aaatcatagt taatttacta aaaaaattaa
    23281 gtaaggccgg aaaattaatt ttagtcgtcc atcacgattt gagtaaagtt gatcattatt
    23341 ttgatcaagt aattattttg aaccgtcatc taattgcttg tggaccaata gatcaggcat
    23401 ttacccgtga gaatttaagt gctgcttacg gtgatgctat tttgttagga caaggagact
    23461 agaatgttta cgaaattttt tgaaggctta ttgacttatc attttttgca aaatgctttt
    23521 ataactgcaa ttgtgattgg tattgtagcg ggtgcagtag gctgttttat tatcttgcgc
    23581 tcaatgtctt tgatgggtga tgctatttct cacgctgttc taccaggtgt tgctatatct
    23641 tttattttag ggattaactt ttttataggt gctattgtct ttggtttgtt gtcttcgatt
    23701 atcattactt atattaaaga aaatagtgtc ataaaaggag atactgctat tggaattact
    23761 ttttcgtcct ttttagcctt aggtatcatt ttaattggac ttgctaatag tacaactgac
    23821 ctctttcata ttctttttgg gaatattttg gcagtacaag atagtgataa atatatgact
    23881 attattgtgg gattaattgt actgactctg attactatct tctttaaaga attattattg
    23941 acttcttttg atcctgtttt agcaaaatca atgggaatga gagtttcttt ttatcattat
    24001 ttattgatga ttttgttaac attagttgca gtaacagcta tgcagagtgt gggaacaata
    24061 ttgatcgtag cacttttaat tacaccagct gcaacagcat atctttatgt taaaagtctc
    24121 agaacaatgt tgtttttatc atctgcatta ggagcagtag catctgttct ggggttatat
    24181 atcggttata catttaatat tgccgcagga tcaagtattg ttttaacatc tacttttatg
    24241 tttttacttg ctttcctatt ttctcctaaa caaagtttgt ttaaaaagaa acaatagtgt
    24301 tgttaaaaaa tgagactaga aaatgttcta gtctcatttt tgatagaatt atttaaattt
    24361 gtagtcttta ataactttga tacttgtaat agtaattttt tctttcggtt gatctgattt
    24421 tgtaacttct accgaagcaa ttttatccac agtttccatg ccagagatta cttgtccaaa
    24481 gacggtataa ccaccatcta aacttggatt tcctccctct gaataggctt taataatcac
    24541 cttaggaact tttttatctg acagttgttt ggaatgatct tgctggcttt gattgataaa
    24601 gaattggctg ccattagtat cagctccagc atttgccatt gctaaacttc ccctgatatt
    24661 gtagaggtaa ggggatattt cattaacaaa accatttcca gaatctttct ttttatcttt
    24721 gctattccat atagattttc caccggtccc gtctccatta gggtcaccag attgtatcat
    24781 aaaatctttg ataactctgt ggaaacttaa cccattatag tatccttctt ttgcatgtgt
    24841 tagaaaattt tcaacagcta aaggagcata ttttggaaat aacttgatat ttatatcacc
    24901 tttagaggtt tttataagca cttgtgcctc gtttttggca acagccttgt ccaattgagg
    24961 aaaagctttt ttatcagctt ttaaggcttg cttagttttt ttgtttagtt gttcagtagc
    25021 ttcctttgaa gagttttcag ctagtttttg gtcaacataa cggtctcctt ttagagaacg
    25081 ctcaattgac tcgcaaccac ttaaagttaa tatactgaca caggctaaac caagataaat
    25141 aatttttttc atacatactc cttgattaag ttattaagat gattatattt tatcataaat
    25201 ttttaaataa tttataaaag taaatagtaa acctattggg ggacttgttg aaattatgat
    25261 agaatagtat agcatgagtt gatagagaaa gtgaggaaca agtatttcac ttgaaatatg
    25321 gtatttatgg caaataagaa aaaaacaaaa ggaaagaaaa ccagaagacc tactaaggca
    25381 gaaatagagc gtcaaagagc tattcaaagg atgattactg ctcttgtttt aacaattatt
    25441 ctcttctttg gtattatcag attaggtatt tttggtatta cagtctataa cgtcatccgt
    25501 tttatggtag gtagcttggc ttacttattt attgcggcaa ctttaatcta cctttatttc
    25561 tttaaatggt tgcgaaagaa agatagctta gtagcaggtt ttttgatagc ttctttagga
    25621 ttattgattg agtggcatgc ttaccttttc tcaatgccta ttttgaaaga taaagaaatt
    25681 ttgcgttcaa ctgctcgatt aattgtgtct gatttaatgc aatttaaaat cactgttttt
    25741 gccggtggag gtatgttggg tgctttgatt tacaagccaa ttgcttttct cttttctaat
    25801 attggtgcct atatgattgg tgttctcttc atcattttgg gtctcttttt aatgagttct
    25861 ctggaagttt atgacatcgt cgaatttatt agagctttta aaaataaagt ggcagagaag
    25921 cacgagcaaa ataaaaagga gcgttttgct aagcgagaga tgaaaaaagc aatcgctgaa
    25981 caagagcgca tagagcgtca aaaagctgaa gaagaagctt atttagcttc ggttaatgta
    26041 gaccctgaaa cgggtgagat tctagaggat caagctgagg acaatttgga tgatgcgcta
    26101 ccacctgagg taagtgaaac atcaactccg gtatttgagc cagagatcct tgcttatgag
    26161 acatcgcctc aaaatgatcc tttaccagta gagccgacaa tttatttaga agactatgat
    26221 tcgccgattc ctaatatgag agaaaatgat gaggaaatgg tttatgattt agatgatgat
    26281 gtagatgata gtgatataga aaatgtcgac tttacaccta aaacgacact ggtttataaa
    26341 ttaccaacga tagatttatt tgcaccagat aagcctaaaa atcaatccaa agaaaaggat
    26401 ttagtccgaa agaatatcag agttttagaa gaaacattta gaagttttgg tatcgatgta
    26461 aaagtagaac gtgctgaaat tggaccatca gttactaaat atgaaattaa accagcagtt
    26521 ggagttcgtg tgaatcgtat ttcaaatcta tctgacgacc tagctcttgc tcttgcagca
    26581 aaagatgtgc gtatagaagc accaattcct ggaaaatcat taataggtat tgaagttcct
    26641 aactcagaaa ttgcaacggt ttctttccgc gaactttggg aacaatctga tgccaatcct
    26701 gaaaaccttt tagaagtacc actaggaaaa gctgttaacg gcaatgctcg cagttttaac
    26761 ttagctagaa tgccgcatct tttggtagct ggttcaactg gttcaggtaa atctgtggca
    26821 gttaatggaa ttatttcaag tattttgatg aaggcacgtc cagatcaagt taagtttatg
    26881 atgattgatc ccaaaatggt tgaattatct gtttataatg atattccaca tttattaatc
    26941 cctgttgtaa ccaatccgcg taaagcaagt aaggcactcc aaaaagttgt tgatgaaatg
    27001 gaaaatcgat acgagttatt tagcaaaatt ggtgtgcgta atatagcagg ttataataca
    27061 aaggttgaag agtttaatgc ttcctctgag caaaaacaaa tacctttgcc tttaatcgtt
    27121 gtcattgtag atgaattggc tgacttgatg atggttgcta gtaaagaagt tgaagatgct
    27181 attattcgtt tggggcaaaa agcacgtgct gcaggtatcc atatgattct tgcaactcaa
    27241 cgtccatccg tagatgttat ttctggtttg attaaagcaa atgttccgtc gcgtattgca
    27301 tttgctgttt caagtggtac tgatagccgt acgatccttg atgaaaatgg tgctgaaaag
    27361 ctcttgggac ggggtgacat gctctttaag cctattgatg agaatcatcc agtacgacta
    27421 caaggttcct ttatttcaga tgatgatgtt gaaaggatcg ttggttttat caaagaccaa
    27481 gccgaggctg actatgatga tgcctttgat cctggagaag tatctgaaac agataacggc
    27541 tctggtggtg gcggcggagt acctgaaagt gatcctcttt ttgaagaagc caagggactc
    27601 gttttagaga cgcaaaaagc aagtgcctca atgattcaac gccgattgtc tgttggtttc
    27661 aatagagcaa caagactaat ggaagaatta gaagcagcgg gggttattgg tccagcagaa
    27721 ggaaccaagc cacgaaaagt tttaatgact ccaactccga gtgaataaga aaaaactaat
    27781 ctgacaagaa attgtcggat tagtttttta tgttataatg aagcaaatag atgaagaaga
    27841 aggttacgaa tgcatattga gactgttatt gatttcaaag aattaggaaa aagatatcgt
    27901 tttaaaaatc ctacaaaaga attaatagct gatactttag aacaagtctt agaagtgata
    27961 aaagaagttg attattatca atctcaaaat tattatgttg ttggttattt atcttatgaa
    28021 gcatctgctg cttttgattc acattttaaa gtttctcaac agaagttggc tggagaacat
    28081 ctagcttatt ttacagtaca taaagattgt gagaacgaag cttttccttt aagttatgaa
    28141 aatgttagat tagcagataa ttggactgct aatgtttctg agcaagaata tcaagaggca
    28201 attgctaata ttaaaggaca aattagacaa ggaaatactt atcaagtaaa ttatacacta
    28261 gagcttagcc aacaattatg ctcggatccc tttagtgttt atgaacgcct aatggtagaa
    28321 caaggagcag gatataatgc ttatattgcc tacgacgata aaaggatttt atctgtaagc
    28381 ccggaattgt tctttaagaa gaaggatgaa gtcttaacaa ctagaccaat gaagggaaca
    28441 agcgctagaa agcctactta tcaagaagat gtggcggagc gagattggtt ggcaaatgat
    28501 cctaaaaatc gttctgaaaa tatgatgatt gttgatttat taagaaacga tatgggccgt
    28561 atctgtgatg ttggaacagt taaggtaaaa aaactatgtc aagtggagca gtatgcaact
    28621 gtgtggcaaa tgacatcaac tattgaagga gttttatcgc cagaagtgac acttatgtct
    28681 atttttcaag ctctatatcc ttgtggatct atcactggag ctcctaagat atcaacaatg
    28741 gctattatta atgagttgga aaaacggcca agaggtattt attgtgggac gataggactt
    28801 tgcatgccag acggacaagc tatttttaac gttcctattc gcacagtaca aatgaaaggt
    28861 caacaagcct attacggcgt tggtggaggt attacgtggg agagccagac agattctgaa
    28921 tatgaagaaa cccgtcaaaa atcagctgtt ttaacacgtg tcaatccaaa atttcaatta
    28981 ataactacag gaagagtgac tgaaaataaa cttcttttct ctcaacaaca tgtcgagaga
    29041 ttagttgaat cagcgtctta ttttgcttat tcttttgata aaagtaaatt cgaaagggaa
    29101 ctgaaaaaat accttcatca gctagatgag aaggattacc gcttgaaaat aatgcttgat
    29161 aaaactggta aggtaacgtt tgaggtaaaa caactagtga atttatcaaa aaagttttta
    29221 acggcagagg tggtcgtaca agactaccct attaaattat ccccgtttac ttattttaaa
    29281 acttcttatc gcccacatat tattgaaggt cagaatgaaa agatatttgt atctcctgag
    29341 gggttgctat tggaaacaag tattgggaat attgttttag aaaaaaatgg aaggttttta
    29401 accccagatt tatcagaagg agggttgaat gggatttatc gtcgtcatct ccttaaaaac
    29461 caaaaagtaa ttgaagcacc actaacttta aaagatttag aatcagccga tgctatatac
    29521 gcctgtaatg ctgttagagg gctttatcct ctaaacctaa agtaaaaaga gttggaaatt
//
LOCUS       DATRSG010000019        28012 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRSG010000019 DATRSG010000000
VERSION     DATRSG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28012
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.019"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(595..2190)
                     /gene="cls"
                     /locus_tag="VBD89_000824"
     CDS             complement(595..2190)
                     /gene="cls"
                     /locus_tag="VBD89_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO7135369.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            complement(2309..3979)
                     /locus_tag="VBD89_000825"
     CDS             complement(2309..3979)
                     /locus_tag="VBD89_000825"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO7135370.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAVGLF"
     gene            complement(4068..5087)
                     /locus_tag="VBD89_000826"
     CDS             complement(4068..5087)
                     /locus_tag="VBD89_000826"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7135371.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVLFS"
     gene            complement(5114..5992)
                     /locus_tag="VBD89_000827"
     CDS             complement(5114..5992)
                     /locus_tag="VBD89_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO7135372.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(5961..6779)
                     /locus_tag="VBD89_000828"
     CDS             complement(5961..6779)
                     /locus_tag="VBD89_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO7135373.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(6772..7104)
                     /locus_tag="VBD89_000829"
     CDS             complement(6772..7104)
                     /locus_tag="VBD89_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO7135374.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(7133..8119)
                     /locus_tag="VBD89_000830"
     CDS             complement(7133..8119)
                     /locus_tag="VBD89_000830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO7135375.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(8116..9315)
                     /locus_tag="VBD89_000831"
     CDS             complement(8116..9315)
                     /locus_tag="VBD89_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO7135376.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(9308..10144)
                     /locus_tag="VBD89_000832"
     CDS             complement(9308..10144)
                     /locus_tag="VBD89_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO7135377.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSR
                     DE"
     gene            10283..10981
                     /gene="coaB"
                     /locus_tag="VBD89_000833"
     CDS             10283..10981
                     /gene="coaB"
                     /locus_tag="VBD89_000833"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO7135378.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKI"
     gene            10978..11520
                     /gene="coaC"
                     /locus_tag="VBD89_000834"
     CDS             10978..11520
                     /gene="coaC"
                     /locus_tag="VBD89_000834"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO7135379.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            11566..12138
                     /locus_tag="VBD89_000835"
     CDS             11566..12138
                     /locus_tag="VBD89_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7135380.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            12248..13966
                     /locus_tag="VBD89_000836"
     CDS             12248..13966
                     /locus_tag="VBD89_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7135381.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(14030..14227)
                     /locus_tag="VBD89_000837"
     CDS             complement(14030..14227)
                     /locus_tag="VBD89_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135382.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(14241..15974)
                     /locus_tag="VBD89_000838"
     CDS             complement(14241..15974)
                     /locus_tag="VBD89_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135383.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLSLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(15975..17696)
                     /locus_tag="VBD89_000839"
     CDS             complement(15975..17696)
                     /locus_tag="VBD89_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135384.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(17708..18310)
                     /locus_tag="VBD89_000840"
     CDS             complement(17708..18310)
                     /locus_tag="VBD89_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO7135385.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(18312..19289)
                     /locus_tag="VBD89_000841"
     CDS             complement(18312..19289)
                     /locus_tag="VBD89_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO7135386.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEHFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(19294..20550)
                     /locus_tag="VBD89_000842"
     CDS             complement(19294..20550)
                     /locus_tag="VBD89_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO7135387.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(20642..21238)
                     /locus_tag="VBD89_000843"
     CDS             complement(20642..21238)
                     /locus_tag="VBD89_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO7135388.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(21231..22061)
                     /gene="prmC"
                     /locus_tag="VBD89_000844"
     CDS             complement(21231..22061)
                     /gene="prmC"
                     /locus_tag="VBD89_000844"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO7135389.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(22061..23140)
                     /gene="prfA"
                     /locus_tag="VBD89_000845"
     CDS             complement(22061..23140)
                     /gene="prfA"
                     /locus_tag="VBD89_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO7135390.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(23175..23744)
                     /locus_tag="VBD89_000846"
     CDS             complement(23175..23744)
                     /locus_tag="VBD89_000846"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO7135391.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            23882..24064
                     /locus_tag="VBD89_000847"
     CDS             23882..24064
                     /locus_tag="VBD89_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO7135392.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            24213..25151
                     /locus_tag="VBD89_000848"
     CDS             24213..25151
                     /locus_tag="VBD89_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO7135393.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            25169..25771
                     /locus_tag="VBD89_000849"
     CDS             25169..25771
                     /locus_tag="VBD89_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7135394.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            25795..27030
                     /locus_tag="VBD89_000850"
     CDS             25795..27030
                     /locus_tag="VBD89_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7135395.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            27129..27917
                     /locus_tag="VBD89_000851"
     CDS             27129..27917
                     /locus_tag="VBD89_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO7135396.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
BASE COUNT         9090 a         5326 c         4413 g         9183 t
ORIGIN      
        1 aagttaattt gttatttaat tacaatttat tattaatttg tatgcttaaa attaaatagt
       61 tgatatctaa aacatgatat ttttaaattt tcctttaata ttataaaaca tgatagatag
      121 tcatactaac attaggattt acaatgagat aatagtatat ttttaatact tcatctaaac
      181 catcagaaac tagattattt aaaaatttta caaagaatgt gatggtagca tattcaatta
      241 aagttaagta aaaacataat ttttagaaag aaattgtgac ttatttatgc aaaaaaaaga
      301 aagaaccaaa ctggttcttt cttttttgct tttcagctac ttaccacagt ttgtaatgaa
      361 ttacaagtag tagtaagcta agggcacctt ttcaagtgtg ataagaaggt tcacacaact
      421 tatcaagatc cgctcctgcg tttataatct ccctgcgcaa tagtggtaaa accacatatc
      481 gactcatcgc ataacagtag tttaacataa tttagttaaa atgcaacctt tactttttaa
      541 atataactag attcttcagt ttacttttaa tattaaatta aaaataaatt atttctaaag
      601 caggggagca atagtttttg tcactatgcc aattactttt tggaattttg gaatattatt
      661 tatatctttc aagctaacta accttgattt atttaacgta tccatataat ctcgataaat
      721 gtcttggatt gcatctacct tataaagata aacagcacat tcaaaatggt gatacaggct
      781 acggtaatca agatttatcg tacctacaac agcctttgta ttatcactaa ggaatatttt
      841 tgaatgaaca aatccagggg tgtattcata tattttaaca cctgatttta ttaatgcttg
      901 atagtaagtt ttagcaagag cataagggat tggcttatct gggatacccg gcataataat
      961 acgtacatct acaccacgtt ccgcagcaaa ctgaatggca tgttccaatt cactgtctaa
     1021 tattaaataa ggtgtcatga tgtaaacata ttctctagca tgattaagaa tatctatata
     1081 aacattttcc cctactttat cagtgtctaa aggagaatct ccataaggaa taacatagcc
     1141 ttcattttca actaaatcat cgtgtacacc gaggtaaggc gctactaaca ttttttcctc
     1201 agtaatggac cacatctgta agaaaagtag gaggaaacta tccactgctt tcccctttaa
     1261 cataatacct gaatctttcc aatgcccaaa taactcaatg tgattaatat actcatcggc
     1321 taagtttaca ccaccagtca tgcctactac tccatcaata acaacaattt ttcgatggtc
     1381 acgatagtta taataagttg aaataaaagg agaaatcgga gaaaatgctt ttgcttttat
     1441 tcctattttc tcaagtcgtt tcgtataatc aaaggataat tttgtaattt ctatcatacc
     1501 atcatataag atacggactt caacaccttc ttccaccttt tgttctaaaa tactaagtat
     1561 ttctccccac attattcctt cgtcaatgat aaagaattct aaaaagatat acttcttagc
     1621 ttttagtagt tcttctttca ttttttcaaa gaatgcttct cccgttggta gaaatgtcac
     1681 atcagtattt cgataaactg ggaaatttcc atgtgctttt tcaaaatatt gaaccaaatg
     1741 atatgttgtg gatgtactcg acttaagctg attcaaagtt tcaggatcat cctctagata
     1801 aatctgactc tcatcaatca atgtctccaa tctttgtttc aaaccacgat aaccccagtc
     1861 gaatttggtg tacattagga acatcgcccc tagtaaaggg gcaatcatta ccaatattaa
     1921 ccaagttact cgggacaaag catccatttc actattaact aaatataaga ccgctccaat
     1981 tgttaagata tgctcaacgg ttgctaacca aacacgatac tgctctagcc atgagtaaga
     2041 ggctagtaaa aacaataatt gtaaaatcaa taaaatagcg ataacggttg ttcgactaaa
     2101 tatacccctt agtaaacttt tcttactctt attaagtaaa cgaacaattt tgactttatt
     2161 actgataagt ttttcccccc tccaaatcaa tcattttatt atagcatact gtcacttgaa
     2221 tagcattcta ttattctaaa ttatctctag ttaaaatcta atccccaata aaaagaggct
     2281 agcatcacaa gccctacctc tttattattt aaaataatcc aacggctgta ccgtcttcta
     2341 aaacatccat attgagagct gcaggttttt taggtaaacc aggcattgtc aatacgtctc
     2401 cagtaagagc aactataaaa ccagctcctg tttttggaac aaattcacga acagttatat
     2461 caaagtcagt tggagcacca aataaatttg gattatcaga aaagctatat tgtgtctttg
     2521 ccatacaaat aggcatcttg tcccagccat tgtcactaaa ctcttttaat tgtatttgtg
     2581 ccttaggtcc aaaatgaact ttattaccac catatatttt agtaacaatt tttttaattt
     2641 tttcttcaat agtgtcttca tcattatata agcgtttgta attggatgat tgtgtttcaa
     2701 tcacattagc aacagtctgt gcaagttcaa ggccaccgtc tgctccatct tcccacacac
     2761 ttgctaattc aactgccaca tcaatattac tacataaggt tttaagagtt gcaatttcac
     2821 tttctgtatc tgcagtaaac tgattgatag ctacaactac cggaacacca tattgacgca
     2881 tattgttaac gtgacgctcg agatttgtaa aacctctttt aactgcttcg acgttctctt
     2941 gtgagagatc ttcttttgaa acacctccat gcattttcaa tgcacgcaat gtagcaacaa
     3001 taacaatcgc atcgggagaa gtcggaaggt ttggcgtttt tatatcaaga aacttttcag
     3061 cgcccaagtc tgctccaaaa ccagcttcag taacaacgta atctgctaag cgtagagctg
     3121 ttgaagtcgc taagacagaa ttacatccat gtgcaatatt agcaaatggt cccccatgga
     3181 caagagccgg tgtcccataa atggtttgaa ccaaattagg tttgatagca tcttttagga
     3241 taagtgtgag agctccttca atcttcaaat ctttaacata gattggttta cggtcccttg
     3301 agtaagccac aacaatattt gacaggcgtt tctttaaatc tgataggtca gtagctaaac
     3361 atagaatagc cattatttca gaagcaacag taatatcaaa gccatcttca cgtggtatcc
     3421 cattgacagg gcttcctaag ccaacgatca cctgacgaag agcacgatca ttaagatcaa
     3481 ctacacgctt ccaaatcact cgacgttggt caatatctaa ttcattgcct tgatgaatat
     3541 gattatcaag tagagctgat aaagcattat tagcagttgt gattgcgtgc atatcaccag
     3601 taaaatgcaa attaatatct tccattggta gtacttgtgc gtatccacct ccagcagctc
     3661 ctcctttaat acccattaca ggacctaaag aaggttcgcg aagcgcaatc atcgtttttt
     3721 taccaatttt atttaaagca tcagctaatc caatgctcat agtggatttt ccctcacctg
     3781 ctggtgtcgg attaatagca gttactaaaa ttaactttcc tactttttga gatcttacag
     3841 cttcaatctt atcaaaagat aattttgctt tgtatttacc atacaattct atatcatcaa
     3901 aaccaattcc tacttgctca acaatctcag caataggctt tagggcgacg ctttgcgcta
     3961 tttcaatatc tgttttcatg atattctcct tctaaataat aaaaatagta tagcaatatt
     4021 ccctataaaa acatattttt ttgcaaaaaa aatttaaaat gttcgtctta tgaaaataaa
     4081 actgctttta tttcttctgg actagtctga ccaaaataat cttctaatga taaagtactt
     4141 aatttttcca agactaattc ttctttcata gggcaaccaa tgagaaggtt ttcaatatct
     4201 gaaatatctt tagaactgaa aaagtcacca taaatacggc aattcgtaat aatacctttc
     4261 tcaatttcta agtgaaaatc gtaagttcct gacggaaaac gagcatctct gtgatacgaa
     4321 tactgtggtg agttaccata attccaatcc caattcttat atttttcatc agctagagcg
     4381 tcaatcgcag cccaatcttt ttcggttaga tgatagcgtt ttgcctgatc aatatgatca
     4441 atatgtaata attgacaaac cattaaatct ttaaattgat cggttgtgat atgacgataa
     4501 tcttctgaca agtggctacg aatatctcct actcgacttc ggacagattt tatcccttta
     4561 gattcaattt ttttccgatt aggatttaac actttttcca tagcatcaaa atcaacatct
     4621 aataaaagag agtaaccacc gtaaatacga ccgtttacta tcgtcattgc tgcacctgat
     4681 acttttttac catcaataac taagtcattt cgtcccctca tttcaacact agaagcacct
     4741 aaagttttta gggccttaat agctggctca taagctcgtt tgaaatcacc aaattgtcca
     4801 tgatctttca ttagatagca aatatttaca gcacctgagt caacgtaaac agctcctcca
     4861 ccagtatctc gacgaacaac aggaatatca tgttctttca aatattcttg atttgtctct
     4921 ataactgcat tctgaaattt tccgatttca actttaggat cacaatagta tggaaataaa
     4981 atatcatcat ctaaaaaaac attttgtaaa atataaactt gcatggcaag tgcaatcgca
     5041 ccgtctgtga tacgcttacc atttctaatt ggttcaataa gatacattta ggtctccttt
     5101 tatagcaatt tagttatcca ataatttctt ttttcgcttc agcaattaat tcagcgatat
     5161 gctgtgtcag ttgcacactc cttgagcgaa tcttttgagg aatgcgataa tgcttgtgat
     5221 taagagtaac aaataaagag ttttcattta aagagacgaa tcgccaaaac ggatgcttaa
     5281 taaattgtgg cgtggtatgt ccaactccaa tctctagata aagaactttg tcattttgat
     5341 gttgtgataa gaaattttca taacgttgtc gttgagcatg aaagtcagca tcttccacca
     5401 tacctttttc ttcgttacgt ttattaattt caaacggttg atcacacttt gggcatttgg
     5461 ggattaagtt actaggaatc ttcatatcct tttgttgtgc aatcatttga cgaattgcct
     5521 gatcatttcg ataagtttgt tgatgacaat gttgactaca ctgccataag ccatattcac
     5581 cttgaatatg aaaaactttt tctaaattat aatcagccac agcgaaggca ttatcagcat
     5641 tagttgtaat aatatgatat tcttttttcg ctaaaatatc ctttaaatca agataagctt
     5701 gtcctaccgg ttgatctaaa taattaagtg ccacaaaacg actttggaag gcccaatatt
     5761 cttcccaatc ttcaaagtca taaagacttg cttgcaacat atctaatagt tgatatttag
     5821 caataaaatc cggaaaagct tcttcaaatc tggggccaat ataggtaaat ccatccgctg
     5881 cagacatgcc tgcaccaata ccaactacaa tagcatctga ttcttctatt aactgactta
     5941 gtatttctga ttgagaatga ttagtttttt ctagagtctt ccacactgtc atctatttcc
     6001 tcttttaata ataatttttg ataaatctct tggtctttag gtgtgaatgt attgaaaata
     6061 atatagagtt tgtgctgatt ttcgcgttgc cactctaata tctcttgaac agccaattca
     6121 gcggctcttt gattaggaaa accaaattct cccgttgata tacaagggaa gacaattgtt
     6181 ttaagattag cttgagctgc caattgtaag caggatttat aagaagactt taataaatcc
     6241 tctcttatac gagatggctt ttgatcaact tttacataag gtccgactgt atgaataaca
     6301 tacttacaag gaagatgata ggattcggta agtttagctt gtcctacggc ttccattcgt
     6361 ccttgttgtg tcatcaattg atgacatgct aaacgtaatc gtgaacctgc aaacgtatga
     6421 atctgattgt caatacaatg gtgattagga ataaaacaac ctaacaactt actatttgct
     6481 gcattaacaa tagcatccac ttgtaataag caaatatccc catggtaggt aaatacattt
     6541 gaatataccg tttgatgaca gacgtctatc gtttttactt tttgagtatt ccaccaatgt
     6601 gacaaatacc tatcttctaa agacaggtac tcttgactaa tgttttgagg atcttgttga
     6661 ttaactagac cacgccaaac cgtttgcaaa tcggtggtcc tcaagtcatc aacgtctgtt
     6721 aatttttctg actgaagata ctcaatcatg gctagcaata gttgtttttg attaggcatt
     6781 ttctaaagct tcaaaagcag cataatcaac atctgttaac acaaccaacc aattttcgtc
     6841 agctttttca gaatttaaca aagttggttc ttgacttgca gctgtattaa ctttaacaac
     6901 tttacctgta agaggagata aaattgccat aaccgtcttt gaagcttcaa tatttaaaat
     6961 aacatcatca acttctaaat ttgtatcatc tgtgaattcc acatatccaa cagttcccaa
     7021 gtcatcttga agttcgggag ttaagctgat agtataaagt tcttcatttt tttcaattaa
     7081 taagtagttc gcaatttttt tcatgagtaa tttcctttct gagaaaacgt gattaatata
     7141 actgtgataa taattttaag gatttgattc gttgatcaag tcctggaaca agaggaatca
     7201 aaagtagttc ttctgcttga ctagcattaa ctaaagcgtc aaggctttct ttaacttgtt
     7261 ttggatcacc tacaatcata cggtggcgat ttgatttaat ttttgctttt tgttcaggag
     7321 tcaattggta atgattagct tcttcaatag taggaaatgt tgcaaattca ttgaagtctt
     7381 tatttcctag catccagata tctaaagtct ttgctatgtt ttcagcttct tctgaagtat
     7441 cagctataac tacaaatgga gctaccatca aattaggaga cttattagga taatattctt
     7501 caaactgatg ataataaaga gatgaaagct ttttagcttc tgtcaatggg tctttgtcca
     7561 taaaaggaaa aacaccgaag gtaaagccta agccaagttt agccgctaaa taagctgatt
     7621 tttgaccact ccccaacaca tataagtctg ggaagctaga aagagtcggt tgaactaatg
     7681 gttccttact ggatgattca tcaataagcc atgacttcaa ttcctccagt acctcttcgt
     7741 aatcatgtgc tttatgtaag ctacgaagtg catttgaaac tttaactgtc cctagtgaat
     7801 ttcctaaacc aatacttact cgattcggat gacatgtctc taatgtcttg agagtctccg
     7861 cgagtttaaa actactatag tgcagaggca ttatacctcc agagccaatt ttgatagatt
     7921 tagtctggtt agccaaatgc attatcatta attctggatt gctaatactg aatgccttaa
     7981 caccgtgatg ttcagccacc caaaattgat gatagcctag ttgctccgcc tcttgtgata
     8041 aaagaatcgt ttcttgtatt gcatcacttg cagtttttcc ataatcaata agcccataat
     8101 caaggacact taatttcatc tttcctccct ataattgctt cgaagttctc taaaaacttc
     8161 tcgagcttcc tttggtaaag attctccata atccatcaaa ggaacaatat ctttaaagga
     8221 ggcttttgga atcgccaaac cttctaaatc attcacttta atccttaaat taatctgatc
     8281 agaacgctta gcttttattt tttgtaaaaa ttccggatca actactaagg gcgtggaagc
     8341 tcctatcata tgagcatgtt gtaaggcttc aaacaccttt gaagcactat taatacctcc
     8401 agttgccata attggaactc tatcaccaaa atgttcaaat ataacttgat taaccagctg
     8461 ccctttgtaa actccctcag aacgtatagt atttctaaag acatcatgcc cccagcttgc
     8521 aatggcaaga taatctacct gagcaatcgc taaaattttt tcaagaaatt ccataaactc
     8581 ttcaatgcta tatccaattt gtgaaccgcg agtttcttca ggcgtcgctc taaaaccaag
     8641 gataaaatct gactccgctt cttctctgat aactttttgt accgctttaa aaacttctaa
     8701 gcctaaacga cagcgattag tcaaagtttg aggaccatac tcatcttttc tttgatttga
     8761 gaaggttgag aaaaaagttt gaattagtaa tcgttgtgca gaagatattt caacaccatc
     8821 aaatccagct tgtatagctc gtcttgtagc ctggacatat tcatcgataa tccttagtat
     8881 atctttgtgt gttagttctt taacctgatg tgggtatggc gattgcaatt gcatagggct
     8941 gggaccatat acatatccgt gtcttgccaa agtgtgactt gagaaacgtc cagcatgcgt
     9001 cagctgcaaa acagctttcg ctcccttaga cttcattgct ttagccaatt tagtcaaacc
     9061 aggaatgtcc tcatcctttg aaacgctaaa accatattca aataactgac catattctgt
     9121 gatatatgct gctccagtaa tttgtaatgg agcggattta gcacgacgaa cagcatatgc
     9181 tatgtcgtca tcagttacaa aaccttctga tgtagaagaa ttagttacca taggtgatag
     9241 aacaaaacga ttttctaagc tcaatccatt gggaagtgtt aatggacgaa ataaattacc
     9301 ttggacatta ttcatctctg ctcaccatcc ccttattagc catagactta gcctgaaatc
     9361 tatcaaactc tgcaactaaa tcgggtctaa tcatcagtaa acgatcatta aaacctactt
     9421 gtttaaggct aatcaacata cgatcaataa catcttctac tgtcatagga caatctaata
     9481 gatcagatag attagccata atttcaggat ctacatttgg ataagcaatt ggactacccg
     9541 tatcacctag accaatctta taaaaatctg aaatcatttg actccgccct ttttgatcgc
     9601 cacaaacgct aaggtaaatt gataccgcaa taccattctt tatacggcgc tgagccaagc
     9661 cagcaaattt tttgccattt atactaagat caaacttacc aggacaatag gaggtctcta
     9721 cttcaaagtg ctcaataggt tgataaaaat ccgaaaatat acttctaata aaatcgacca
     9781 ttataagata cccatctgag atagacaatt ttctctcaaa aacatctgga ataaccaatg
     9841 aaaaatttaa aattccttca tcagctacaa ctgcgagacc tccaaaattc cgaacaactg
     9901 gttcataacc acgactgatg atttcttttt tagctaattc taaatgtggt aattctcgat
     9961 ctaacatccc cagaatgact gtcttagtca tcggccaaat atgcaaaata atttcttgat
    10021 ttgagcgatt aatctccctt aaaaatactt ctgtccatat aaaaggtttt tccgcgtctt
    10081 gagcatctgt aacatagtct ttaaaaatag atacaggtaa ctgcgctaga tcttgccact
    10141 ccaataaatc tgacctcctt tctctagaaa actctgcaag atttatttta tcgcaatcgc
    10201 ttgaaatttt ccagtaagtt tccctcaaat tccattacta tcaagaagaa atatatcttt
    10261 tatttacgaa aagcattttt ttatgtacaa tatggctatg aaaattttga ttacatcagg
    10321 tggaactacc gagaaaattg ataccgttag gagtatcacg aatcacgcaa caggagcttt
    10381 aggaaaaata attgcagaaa aatatttacg agaaggacat caggtgacac tcgtgactac
    10441 taaaaatgca gtcaaaccag aatctgctac caacttatcc atatttgaga ttgaggacgt
    10501 tgactcatta ataaaaacct tgaagccatt agtgaaagag catgatattt taattcatag
    10561 catggcagtg tctgactata cccccgtcta catggctgat ttcgaaaagg ttaaatcaag
    10621 cgatgattta gatacttttt tgcgcaaaga taaccacgaa gggaaaatat catctgaatc
    10681 agaatatcaa gtcctttttc tcaaaaaaac accaaaagtt atcagccttg tgaaaaaatg
    10741 gaatcctcaa attactctag taggttttaa actacttgtg aacgttacta aagaaaacct
    10801 ttttaaagtt gctcgtcata gcttaatcaa aaataaagct acgtttattt tagctaatga
    10861 cctcattgat attacctcaa aacatcacat agcctaccta cttgatcatg ataatgtgta
    10921 taaagctacc accaaagaag atattgctca attaatttac gagaaggtaa aaaaaatatg
    10981 attaaacgta ttacactagc cgtcacaggt tcaatttcgg cctataaagc agcagacctt
    11041 acaagtcaat taactaaaat tggctatgat gttcatatca taatgacaca agctgccaca
    11101 caatttatta caccgttaac tctgcaagta ttatcaaaaa atcctattca tttagatgtt
    11161 atggatgagc ataacccaaa aatcatcaac catatcgaat tagcaaaacg cacagattta
    11221 tttatcgttg cacctgcctc tgctaatact attgctcatc tagcatatgg cttcgctgat
    11281 aacatcgtaa caagcgttgc tcttgctatg cccgatgaaa ctccaaaact tatcgcacca
    11341 gcaatgaata caaagatgta tcataataca atcacccaac gtaacattga tattttgaaa
    11401 aaaataggtt atcaggaaat tgaacctcgt attagtttgc ttgcctgtgg tgataccggt
    11461 caaggagccc tagctgatat ctctactata cttaaatgta tacaagaagt tgcaaattag
    11521 gctgtaaatt tgtatactat ttctagttta atttaaggag ttataatgaa aaagaaaaac
    11581 aaatcctcta acattgctat aattgcaatc ttttttgcta ttatgcttgt cattcatttt
    11641 ttgtcatcat ttatttttag tttttggtta gtccctatta aacctacttt gatgcatatc
    11701 ccagttatta ttgcatctat agcctatgga cctcgtattg gtgcaactct aggcgcctta
    11761 atggggggga tcagcgtagc taacagcagc attgttctat taccaacgag ttacctcttc
    11821 tcaccttttg ttgaaaatgg taatttttat tcgctaatta ttgcacttgt accacgtatt
    11881 ctaatcggga ttattcctta tttcgtttac aaattactac acaaccgctt tggtttggct
    11941 atctcaggtg ctataggctc tctaacaaac acagtatttg ttttatctgg aatttttatc
    12001 tttttttcaa gtacttataa tgggaatatc aagctaatgc tcgctgggat tatttcatct
    12061 aattcattag ctgagatggt cattgcagct atcattgtat atctaactgt tcctcgtatt
    12121 ctcaatatta aacattaaaa actggccagg aagaaatctt cctggctttt cttacttata
    12181 ttttatagtc atattgaaaa aaacatgtta taatgaaagc gtttataaat attgaaggag
    12241 tatttcaatg acttacacag aaaattttca aaaatggtta gattttgagc agcttcctga
    12301 ctatcttcgt caagaattac tatcaatgga tgaaaaaaca aaagaagatg ctttttacac
    12361 aaacttagaa tttggtactg ctggtatgcg tggttatatt ggtgctggta caaatcgtat
    12421 taatatttat gttgtccgtc aagcaaccga aggattggca aaattaatcg aaactaaggg
    12481 agaggaagct aaaaaacgtg gtgtagccat tgcttatgat tcacgccact tctctccaga
    12541 atttgctttt gagtctgcac aggtcttggc acagcatgga attaaatctt acgtatttga
    12601 agctcttcgt ccaacaccag aactctcttt tgctgttcgc catttaaatg cctatgctgg
    12661 tattatggtt actgcaagcc acaatccagc acctttcaat ggttataaag tctatggaca
    12721 agacggaggt caactaccac cagctgatgc tgatgcacta acagacttta tccgtgctat
    12781 tgaaaatcca tttgcagttg aacttgctga tttagatgaa agcaaatcat cgggccttat
    12841 tcaggttatc ggtgaagatg ttgatattga atatcttaga gaggttaaag atgttaatat
    12901 caatcaagat ttaatcaata actttggaaa agacatgaaa attgtctata ctcctctcca
    12961 cggaactggt gagatgttga cacgccgtgc ccttgctcaa gccggttttg aatctgttgt
    13021 tgtagtagaa tcacaagcta aggcagatcc agacttttct actgttaaat cgcctaaccc
    13081 tgaaagccaa gctgcctttg ctcttgctga agaactcgga cgagaagtta atgcagatgt
    13141 cttagtagct actgatcctg atgcagaccg cctaggcgtt gaaattcgcc aaccagatgg
    13201 ttcttataaa aacctttctg gaaatcaaat tggagcgatt attgcgaaat acatcctgga
    13261 agctcataaa acagcaggaa cacttccaga aaatgctgct ctcgctaaat caatcgtatc
    13321 aacagaatta gtaacaaaaa ttgccgaaag ttatggcgct acaatgttta acgttttaac
    13381 tggattcaaa ttcatcgctg agaaaattca agagtttgaa gaaaaacata accacaccta
    13441 tatgtttggt tttgaagaga gcttcggata tcttattaaa ccatttgtcc gtgataaaga
    13501 cgctattcaa gcagttcttt tggttgctga gattgcagct tattaccgtt cgcgtggttt
    13561 aacacttgca gatggtattg atgaaattta caaagaatat ggttattttg ctgaaaaaac
    13621 gatttcagta acactttcag gagtggatgg agctgctgaa attaagaaga ttatggacaa
    13681 attccgtgaa aatggtccaa aacaatttaa caatactgat attgtattat tagaagattt
    13741 ccaaaaacaa atcgctacta aaaatgatgg aacaatatct aacttaacaa ctcctccaag
    13801 taatgttttg aagtacacgt tagctgatga ttcatggatt gcagttcgcc cttcaggaac
    13861 tgaaccaaaa atcaagttct atattgctac ggtcggtaat gatttagcag atgcagaaac
    13921 aaaaatcgcc aatattgaaa aagagattac aacatttgta aattaataaa aaaagctagg
    13981 aacttactcc tagctttttt tatatctatc taacgatgtt ctattatttt caatcatgaa
    14041 atataactgc aattaacttc acgatcaaat aatagagcag acttacaatt aaccacgtta
    14101 tcccaataac tgcaactaaa cctattaaaa cagataatac cgtattctta gttgctagtt
    14161 ggttactgat aaaccatgaa actgcagaaa taaatatcag taataatagt tgaagccttt
    14221 ttttcatata attcctctta ctacttttgt gcattttgca tttgataata aatacctttt
    14281 tgagctatta attcagagtg gttacccact tctacaattt caccagatac cataactaaa
    14341 ataatatctg ctgtttgaat ggtagataag cgatgtgcaa taataaagct tgttctcccc
    14401 ttcatcaaca tttggaatgc ttcttgaaca agaacttcag tacgagtatc tatagacgat
    14461 gtcgcctcat ctaatatcaa tattctaggt aatttaagaa aaatacgagc aattgtcaat
    14521 agctgacatt gcccctgtga taacgaatct ccagcatctt ctaaatacgt atcatatcca
    14581 ttcggtaatt gtttgataaa gaaatctgca ttagctgctt ttgcagcctc tatcacttct
    14641 tcacggctag ctttaggatt ggcatatgca atgttatcgt gaatcgtagc actttttaac
    14701 cacgtttctt gtaataccat tccaatttct tgccttaatt gtgatggctc ataatcagta
    14761 ataggcttac catctaaaag gatatttccc ccgtcaactt catagaaacg cattaataaa
    14821 ttgattaaag tagacttccc agcacctgtt ggtcctacaa tagcgacctt agctcctgct
    14881 ggaatgtgta aattaatacc attcaatagt ggttttgatt tattataacc aaaaataacg
    14941 tttttaaaat cgatttgacc tttgaccgtt gatgaatcaa gtttttctgt acctgttata
    15001 tttggtgacg attcttctaa aatagagtaa aggcgttctg cacaagccag agcactttgc
    15061 atctcagaca ggacggaaga gatgtcatta aatggcttag tatattgatt gacataattt
    15121 aagaaagtga ttaattgacc aacactaaaa gcacctgaca taatacgaag ggcaccaatc
    15181 cctgctaaga agccataaat caaactattg ataaatcggg ttgatggatt gacagttgaa
    15241 gaataaaaaa ttgctgattg agaaaagtta gcatactctt gattaatagt cctaaaatga
    15301 tcactacttt cttcttgcgc actgaatgct tgaataaggc tttcttgact caccatctct
    15361 tcaatgaatt gagtttgtcg tcctcgtgat gccgtttgat tttgatataa gtgataactc
    15421 tttttagcaa taaagcgggc tagaaagagt gataaaggcg tcagaaataa taccaaaaaa
    15481 agcattaata aatcaatctt tgccatacta aaaatagtaa ctagaatagt taataaacca
    15541 acaaagaact ggttaaaaac cattaataaa ccatttgata attgctccgt atcggtagtc
    15601 acacgactaa tcaagtcacc tatgccacgt ttatcaaggt aagctatagg taatagactc
    15661 aacttttcca ttacagcctt tcttaaagaa gcaacataat gaaaaattaa gcggttatag
    15721 agcagaggat taatccattg aataatagta tttgctaaaa taactgcaac catcttccac
    15781 attataggta acaataatat catagaatga ggcgataata caacgtctac cgcctgtcca
    15841 attagaacag gcaaataaac agtgaggcaa acttgtacta ccgtccctaa taatgccatt
    15901 cctactaagc tttttttaga aagaagatct tgaacaagct ttttttgtat ctttttagga
    15961 tgacttgctt tcatttactc ctcctcctta aaattttgtg aatggtaaat ggacttataa
    16021 actttgttat gagctaataa agattgatga ttatcaaaac caacttgttt gcccttatca
    16081 aggagtaaga ttctatctgc attttgtata ctattaattc tctgtgatac aataataaga
    16141 ttagtttggt tgaaatgttt aattatagct ttaaataaac gagcttctgt taaataatcc
    16201 aatgcagatg ttgcatcgtc taaaatcaaa aatggaatct tatcttgaac taaagctctt
    16261 gcaatcgtca acctttgcct ttgaccacca gagaaattcc taccaaaact ctctacaggg
    16321 gcatcaagtt gaccatcttt ttcttttaca aaatcactag cttgtgctat ttctaaagca
    16381 gtccaaagtt tttcctcact aactttacca agtcccaaag aaaggttaga acgaatagtt
    16441 cctttaaaca actgagcatt ttgaggaaca actcttacca ggctacgcca atttgaaatt
    16501 gtatctggac tttttccctg atgatatata tcaatatccc cttcttgcac tttataaata
    16561 tgaagcagta aattaaccaa ggttgatttc ccagagccag ttccaccaat tatccctaaa
    16621 gtttctccta cattcataga aaaagtaata tcagataaag ctttttcaga ggagtttgga
    16681 taagaaaatg ccatttcttg cacttctaaa gccttattcg aatattttgg ctcaagtttg
    16741 tcatcaatta tttcagacgg tctttcaaaa acggctataa ttcgcttagc actgatataa
    16801 ctttggttca gtgatgtcac caacattgtc atttttaaca attcaacaag aatttgcaat
    16861 aagtaattaa ttaaagcaac caacattcct tgtgataaaa gatgattagc tatatttaaa
    16921 ttaccacgcc aaataatcac aaccaaagtg atattaacaa ctagaaatgt tagaggcgtt
    16981 actaaactag aaagcctacc tgcttttaat tgtaaattcg tatagtgata attgatatca
    17041 ttaaatgctt cactctctct atctacttga ttaaaggcac ggataactcg tacaccttga
    17101 agttgttgcc tagtcaactt taccaaataa tcggttgaag ttctgatttt aaggtaaata
    17161 ggatttaata gtcgagacat tacaaaaaca atgataaata acgtgacaac cattatcaag
    17221 aaccaaatag tcaatgaggg actaatggaa aaagccataa ttatggcacc aaaaacaata
    17281 atcggagccc tcaaaaataa acgtaaaaat tgatttaaac cagtttggat gtgaaatgta
    17341 tcagcagtca aacgagtaat caaactagca gttcccaatt catctctgtc ttttttaccc
    17401 agtgacatga ctttttgata gagatcctct gtcaattgtc tcgtataacc aactgcggct
    17461 tttgaagaat aatattgagc agttatcgct actacaacac ccaccgcagc caaaaagaaa
    17521 atcaatccaa tttgcaacag taaactactt ctatcccctc gtggaatggt ctcgtcaatc
    17581 atttttgcaa taacgattgg taccaaaagt tcaaaagagg cttctaataa tttgaaaagg
    17641 ggtccaaaga tagtttcttt gatatacccc ttaaaataat aagttagtct tttcatactt
    17701 acccttctta aaacaactcc attattttta tcaaataaag gttgaattga acttcacgtg
    17761 agtagtaaat attaccacca tttttataga gcttccctcc tgaaattaag gcgagaggat
    17821 gatagtaaaa gtaagtgtcc ccgttataat ttcctaggct gggagctaca acatttttag
    17881 agtaattttt ggccaactca atggaatttt cacccccatg atccgctaca taatcaatat
    17941 aagaagaacc aaaattatat gcttggacag cagtccatac atcaacctta gcttttttag
    18001 cttgacttat attttgtgac aacagagtga cgccatgttt aatactggtg tgactatccg
    18061 taatactgtt cgtagttcca ctgatacttt cacttgattg catcacatct atagcatctc
    18121 ccttagtttc agtataaatc atagataata ctaagtgcac attagcctct gaaccattct
    18181 ctgcaagagt attctgcact aaatcatgat attgaagcac ttttttaaca ttttcatgga
    18241 tgataactag acggtaccca atgaaaataa taataattaa tgctatcaaa cgttttaaaa
    18301 atttaaacat attatttatt ttgaatatcc tgtatgtttt tgattagtat tgagtatgta
    18361 ccatcagggt tttggataaa ttcaactgat tcggcatctt ggtagacatt gttgggtaca
    18421 atcaattcaa taccatttga aagagatagc ttctgattct ctaacttctt aatttgacgg
    18481 gaatgatcaa tatcacttac ttgaacagat ccaggtattg cgtctttcaa ttcatcaaca
    18541 aaagtcagcc tggctgttaa attatcatca aataattgat ctgctaattt ttctggagac
    18601 aattcttgct cttcttctaa attcttataa atagccgatt tcatttttga ttggaaagaa
    18661 aaatcatctt tgttgaaatt ttcagcaatt ttttgagctg tctgttcaac catttttata
    18721 gattttttaa cagactgttc tggttgtact tgtaataaat tttcagaaaa ataattagca
    18781 aatgaaccat tgtgtttgat acgcttttca attaaatagt attgctttga tgacttgtta
    18841 acaaccaaag cttcatctgg agtttgagca gcacttggaa ggttattctg tgttattgta
    18901 attggctgct cttgattttc agaaacatga gcaaaatgtt cctttaatga gattcgaagg
    18961 aaagcaaaat gttccattcc atccttgtca aattgaataa aaactaagtc attcgttttt
    19021 tgatcttccg atatgacaaa ctcttctttc caaagttgcg caatttgaca ggaagaaaca
    19081 tataaatcat cttgtaaatg actcataaat acattatcct ctccaaaata gccacgctta
    19141 gcttcatcag aaaaaacttt acttaatttt ttacgaaagt agtcatcaat ccttggagtt
    19201 agggttaacg gagtatctga tagcactaac tctgtgtcgt taggagaaaa ctgatgtata
    19261 ataatctgtt tgatataaaa atccatcata acattaattc tcatacaaag ggaaagcgtc
    19321 cgttaatgat ttaatttctt gacgaacttc tgtgaggaca tcttgatttt catgattttt
    19381 gagtgcctta atcattaatt cagcgattct tcgagattct tcaactccca ttcctcgact
    19441 agtaattgct ggagtaccaa ttcgaatacc tgatgtttta aatggtgata gtcgctcaaa
    19501 cggtatagag ttcttgttaa gggtgatatt gacttcttca agaacatttt gtgccttttt
    19561 gccattctca ataacttttg tgacgtctac taagaacaaa tgattatcag taccatcaga
    19621 aatcaaatga aagtcatcat cttctttaaa aactttagcc atggcttgag catttttaat
    19681 aatatcctca ccgtatattt taaaggaagg atctagagct tctttcaaag ctacagcttt
    19741 tgcagcaatt acatgttcta atggtccgcc ttggagtcct ggaaagactg cagaattaat
    19801 ttttttagca attgcttcat cattggttaa aatcaaacca cctctaggtc cacgtagagt
    19861 cttatgtgtt gtcgttgtcg ttacatgagc ataaggaata ggactaggat gatgaccaga
    19921 agcaactaaa cctgcaatgt gagccatgtc aaccattaag taagcatcta ctgcgtctgc
    19981 tatttgacga aacttttcaa aatcaataat gcgagaatag gccgacgccc ctgcaacaat
    20041 taatttaggt tgcgtttcct gtgctatttt caaaatgtta tcatagtcta gcatctccgt
    20101 cttaggatca acagagtaag aaacaaaatg ataagttttt cctgagaagc taactgaagc
    20161 gccatgtgtt aagtgcccac ctgctgctaa gtccatccct aagacagtat ctcctggctc
    20221 gattaaagcc atgtaagctg ctgcattagc ctgactacca gaatgtggtt gaacattagc
    20281 aaactcagca ttaaataaca tcttagctcg ttctatagct aaactttcaa ctacatcaac
    20341 acaatctgtt cctccataat aacgatgact aggataccct tcggcatatt tatttgtcaa
    20401 aacagaacct tgtgctgcca taacagcttt tgaaacaacg ttttctgatg caattaattc
    20461 aatattattt tgttggcgaa tttcctcatc atgaatagcc tgccaaagtt cttggtcaaa
    20521 ttctttaaaa ttgtctttat caaaaatcat tttttactcc ttaagctcgt tataactttt
    20581 aaataatggt ttcaatcatt atatttatca catttttact attagttgca agatgattaa
    20641 attaattttc aaaaattaat tcaggtatcg tagccgttag tacctcttgc gtgattgcac
    20701 cttgccttaa aatcttaact ctatcaccag acaaatcaat aatagtagaa tccacacctg
    20761 tcaatgcttt atcatcttca atacctaaaa cttgatgatt aaactgtttt tgaatttctg
    20821 agaatacacg accactatca catccagaaa tattagcaga aggaccaatc aatggaccat
    20881 ggttagcaat catatcaagg gttacagggt gcttaggcat ccgaaatcct acgcttgata
    20941 acccagaatt gatccaatcc ggaacttctt gtgaagcttc caaaataatg gttagcggtc
    21001 ctggtaaaaa agcatcgtac aattgcttca aataggtcgg ttgattttta gaatatttta
    21061 aaatagtctc aaaatcacat atatttaagt tcatagcctt atcacgaggt ctttttttaa
    21121 gctcatagac gtggttcaca gcttcttcac tcaaagcttt ggcaaagatt ccgtagaccg
    21181 tttctgttgg cataataact gcgccatgat cctctaaaat ctgtcccaaa tcattcatta
    21241 tctaatacca ccattctatc tttaccaaat atatctttca aaactctgca acgattgttt
    21301 ggaaaatatc ttttcaataa acttcgtaaa tcatcacctt gtttgtaacc tatttcaaaa
    21361 tataatttac ctctaggttg caaatattct cttgagtttt caatgatttt acgataaata
    21421 gctaatcctt cttcgtctgc aaaaagggca ctatgaggct ctgaagctag cacattcttc
    21481 cccacctcgt ctttgtcatt gtaagaaata tagggaggat tagacacaat aatatcaaat
    21541 ttatctgaaa tgttactaaa gacatctgat tctataaaag tcacttccgc atcacaatta
    21601 ttagcatttt cctttgccaa gtctaatgca gatacagaaa tgtcactagc tagaacatcc
    21661 caagaggggc gttctttttt tagagaaatg gcaattgctc cactacctgt accaatatct
    21721 aaaacagaac aatcttctac tttattctct gataagatta gatcaaccaa ctcctctgtc
    21781 tcaggcctcg gaattaaaac tcgttcatca acaaagaaaa taagatctct aaagtaagct
    21841 ttacctgtaa tatactgcgg tgatctatgt tttttaagct gttgaaaaat attttccatt
    21901 aattgctgat cagtatcact cacactacta ttttgattta aaaggtaatc agttgttgac
    21961 cattgtttta ggtcttttaa tacataaata aaattttcga cttcttcacc acaagcctct
    22021 aacaactgtt cataatgctt gattaactgc gcataattca ttatttattt aaggcttcta
    22081 acttttgagt ctgatcatac ataacaagtg catcaataac ttcatccatt tttccagata
    22141 aaatagtatc taatttttga agtgtcaaac caatacgatg atctgttaca cgattttgtg
    22201 ggaaattata agtgcgaata cgttctgaac gatcaccagt accaactgtt gatttacgtt
    22261 cagcatcttg ttcatcttga gcaatttgcg caaaatggtc agcaacacga gcacgaatga
    22321 ttttcatcgc tttatcacgg tttttttgct gagtacgctc ttcttgcatt tcaactttga
    22381 ttcctgtagg aatgtgtacc atacgaactg cagtagcaac tttattgacg ttctgaccac
    22441 cagcaccaga cgcgtggtaa atatcgacgc gaagatcttt ttgatcaatt tcgtattcta
    22501 cttcttcaac ttctggcatt accagaacag ttgcagttga agtatgaaca cgaccctgac
    22561 tctctgtcac cggaacacgt tgaacacgat gagctcctga ttcatattta agcttagaat
    22621 aaactgactg accagaaacc atagcaacca cttctttaat accaccaaca ccattaacgg
    22681 atgattccat aacttcaaaa cgccatcctt gagtttcagc atatttttga tacattgtta
    22741 aaagatcacc agcaaaaagc gctgcttcat cgccaccagc agcaccacga atttctaaaa
    22801 taatgttttt atcatcgtta ggatctttag gtaatagaag gatttttaat ctttcttcat
    22861 actcttcttt agctgctttt gattctttaa gttcttcttt agccatttct tccaattcag
    22921 catcaccaga agcatccttg atcatttctt cagcatctga aatattttga atgacttgct
    22981 tatattctct ataagctgtg acagtttctc gagtattagc ttcttcacgt gataattcca
    23041 taaatcgctt tgtatctgaa acaacatctg gatcactaag taactcacct aactcttcat
    23101 aacgatcttc aactgcctgc aattgatcat aaatattcat ttttctttgt atctccttta
    23161 aaaccgatta tcttttaaat atctggatta aaataatgtt ttcgacaaac tgggatatat
    23221 gtttcattac caccaatttg tatctgatca ccttcataga caggttttcc gttttctgtc
    23281 cttaaaacca tggttgcttt cttactacag tattggcaaa ttgttttgat ttcatcaatc
    23341 ttatcggcca ataatagcaa atgttttgaa ccttcaaaaa gattgttttg gaaatcattt
    23401 ttcaaaccaa aagccatgac tggtacatct aagtcatcta ctactctagc taaatcgtag
    23461 acgtttttct tactcaaaaa ttgacattca tcaattaaaa cacaataagg cttttgtggt
    23521 aaattctgaa tataggagaa aatgtccata tcatctgaaa ttggaactgc ttcacgacgc
    23581 attccaatac gacttgaaac gactccaaat tcatctcgag tgtccaaggc gcttgtcata
    23641 ataaccacag gttttccttg ttcttcatag ttatgagcaa cttttaaaat ttcaatggtt
    23701 ttaccagaat tcattgtccc atatttataa tagagttgag ccaaagcgga aaatcctact
    23761 ttctatagta ttctacctat tttaccacaa aggctgaagt tttcccagcc attgaaaaca
    23821 gtgtgtactt tttcacaaca ttatgttaca atgatgccca ataacataaa ggagtaatat
    23881 tatgccattt gtaaaaattg acttgtttga gggacgctca caagaacaaa aaaatgaatt
    23941 agctcgcgag gttactgaag tagtatcacg cattgcaaaa gcacctaaag aaaatatcca
    24001 tgtttttatc aacgatatgc ctgaaggaac ctactatcct caaggtgaac ttaagaaaaa
    24061 ataagaaata gtaaaagagg ttacccaggt aacctctttt gatagtcttt gcaagcatat
    24121 ttcttgacaa attgaaaata tttgaaaata agtattgatt ttcaagttag aaaacggtat
    24181 cattagaata tattgctttg ggggtggaaa atttgcaact ttctcatagt ttaagactca
    24241 tgggaacgac tatcgatatt caaattaatt ctaaaaatgc tcaaaaacaa atccaagagg
    24301 tcatagaact cttggagctc tacaaaaatc gctttagtgc aaatgacttt aattcagagt
    24361 taatggctat aaataacaac gctggtatca agcctattca agtacatcca gacctctttg
    24421 aattaatcac aattgggaaa gaacacagct tagctaggcc tagtaaccta aatattgcta
    24481 ttggtccttt agtgcaaact tggagaattg gattttcaga tgctaaacta cctagcccta
    24541 gtgaagtatc agaagctatg attttatctg acccaactca tatacttcta gattcaaaaa
    24601 atcaatctgt ttttttaaat cagataggaa tgaaaattga tttgggtgca cttgcaaaag
    24661 gatatattgc tgacaaaatt atgacctatc tcaaaaatga gatgattgat tctgctatca
    24721 ttaatttagg cggaaacgtt ttagttcacg gagataatcc taatcgttca gaagggtatt
    24781 gggttatcgg cattcaacat ccgaaaaaga aacggggaca aaatattgga gctgtcaaaa
    24841 ttaaaaacca atctgttgta acctcaggta cttatgaacg ccgtctgatt attgacgata
    24901 aggaatacca tcatattttt gatcgacaga ctggttatcc gatacaaaca gagatggcta
    24961 gcatttctat tgtttctaaa caatccgtcg actgtgaaat ctggaccaca aggttgtttg
    25021 gcttatccat taaagaggct cttgatattt taaacgctgt ttcctacatt gaaggcatta
    25081 tcatcactaa agatgaccgt atctatttat ctgatggctt aaaacatcat tttcaactat
    25141 tttatcatta agaaagagga tttctaatat gaaacttatt ggcatcgttg gaactaactc
    25201 aaataaatca acaaatcgtc aattattaca atacatgcaa caacactttg ctgacaaagc
    25261 agaaattgaa ctcatcgaag ttaaagacct tccgttattt aataaacctg ctgataaaaa
    25321 tgttccccaa gtaattttag atattgctgc taaaattgaa gaagctgatg gtgttattat
    25381 tggtacgcca gaatatgatc attctatacc ttcggcctta atgagtgttt tagcttggct
    25441 atcatatggt atttacccac ttctcaacaa acccgttatg attactggtg cttcatatgg
    25501 aacacttgga tcatcacgtg cacaattaca attacgtcaa atacttaacg ctcctgaact
    25561 aaaagcaagt gttttacctg acgaattctt gttatcacac tctctccaag cctttgataa
    25621 agatggcaac ctacacgata tcgaaacaag tcagaagcta gatgctatct ttgatgattt
    25681 tcgtcttttt gttaagatta ctggtaaatt atcaaatgct cgtgatttac ttcaaaaaga
    25741 agcagaaaat tttgactggg aaagtttata agagtataga agggaataac taagatgaaa
    25801 tttgtaggta ttgttggctc aaatgcagag caatcatata atcgtatgtt gcttgaattt
    25861 attcgcaaga attttaaaac caaatttgaa ttggaagtcc ttgaaatcga tgatattcca
    25921 atgtttaatc aagtccaaaa ttgggaagaa agtttccaac ttcgtcttct taataataag
    25981 atcacacgag ctgatggcgt tattattgcc actcctgaac ataatcatac tattacggcc
    26041 gctcttaaaa gtgtgcttga gtggctctca tttgctgtgc atccactcga aaataaacct
    26101 gttatgattg tcggtgcttc atattatgat caaggcacat cacgtgctca aatacacctt
    26161 cgtaaaatcc ttgatgctcc tggcgttaat gcttacacac ttccaggaaa tgaattctta
    26221 ttaggaaaag ctaaagaagc ctttgatgat aatggaaaca ttatcaatcc tgggactgtt
    26281 caatttttag aaacttgtct tgataatttt atcaaatatg taggagttgt atcatcattg
    26341 agaaaaccta aagctattga atctgaagac ctctactgtc agggacaaat cgatacaacc
    26401 attactagta tcgatccaga tgatcctgat tggttagaca aggcagccga gaaagttaac
    26461 gccgttgaag gggatgctta cgttaaacta gacaatggtt tacttactgt taaccaactt
    26521 aatatgtttt taaatgctat gccatttgag ctaacatatg cagatgataa taatcaattt
    26581 ctatattaca ataataatca tccagatcca gatacaatgt taggaaaacg tgttcgtgaa
    26641 caagtaggaa accgtatggc cacagttcat ggtagtttac caccggcacg aatgaaaaat
    26701 gttgaatggg ttatcggtac tcttcgtaat ggaaatcaag aatatgtacg tacagtcgtt
    26761 cctggtacac ctccagaagt tatcaatact cacaactatc aagctatgta ctacccagac
    26821 ggatcttatg ctggtattaa tgaaattatc tttaacttta aaccgtggtt agattggtac
    26881 ctccaaacta ctggacaacg tttggtcggt ggaactactg ctgctcctgg agcttcagca
    26941 gttgacgcta cttctagcgc ttcaactagc acagaggatg ctcccgttga tcaagccaca
    27001 gatgccactt ctggtgcctc tgaacattaa tctcctcgct tttgaggatc tgatacctta
    27061 aaaagatatc aagatcctcc tttttaatat ttaatcaagt taataaaatc acataaaagg
    27121 aatatactat gtcacttttt caagaaaaaa ttgcttacaa ttgcgctaaa aaggaagcgc
    27181 tttataaaga gagtttagga cgctacgcct tgagatcaat gctagcaggg gcttatttga
    27241 caatgagtac tgctgccggt atcgtcgcag ctgatactat tggtaaaatt tctcctgctc
    27301 tatcaggttt tgtatttgct ttcatcttta gttttggact tatttatgtt ttaatattta
    27361 atggtgaatt ggcgacatct aatatgcttt atctcactgc aggagcctat aataaaaata
    27421 tctcttggaa aaaagccata acaattttaa tttattgtac ttttttcaac ctcgttggtg
    27481 cttgtatatt agcttggttg tttaatcaat cgtactcatt ccagcattta acaaatgata
    27541 gctttttagg gcatgtcgtt gctaaaaagt taggaaaacc tagttcagga gctttcctag
    27601 aaggtattat tgctaatatg tttgtgaatt tagctatcct tgcttacatg ttattaaaag
    27661 aagaatcggc aaaaatgacg gttattttat ctgctatttt tatgtttgtt tttctatcaa
    27721 acgaacactt aattgctaat tttgcatcct ttatgttagc tgcttttagc catatcgaac
    27781 atattaaagg ttttactctt ctaaacgtta tccgccaatg gacacttgta ttttttggta
    27841 actggatagg agggggcgtt ttcatcggcc tagcctatgc ttggttaaat aaaacaaaaa
    27901 cttctcacca tgaataaaag aaaaactgag taatttatta ctcagttttt cttttatatt
    27961 ctcatcatga aatcaataac taaaaattaa cttttttgat aaataaagga aa
//
LOCUS       DATRSG010000020        27897 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRSG010000020 DATRSG010000000
VERSION     DATRSG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27897
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.020"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..597
                     /locus_tag="VBD89_000852"
     CDS             <1..597
                     /locus_tag="VBD89_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO7135397.1"
                     /translation="ELFYYKRRERFLKQHGITINHLPMNFFIGGNMKQVWKSVLGDQY
                     DTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHHKGYLLGLASSSTRHDIML
                     ALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIPKQQILIVEDSEKGIAAGI
                     AAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            735..1526
                     /locus_tag="VBD89_000853"
     CDS             735..1526
                     /locus_tag="VBD89_000853"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO7135398.1"
                     /translation="MKFENIIYGIDGSVATIMLNRPDISNGFNIPMCQEIIDAIRLMS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKDAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            1622..2056
                     /locus_tag="VBD89_000854"
     CDS             1622..2056
                     /locus_tag="VBD89_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7135399.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            2056..3027
                     /locus_tag="VBD89_000855"
     CDS             2056..3027
                     /locus_tag="VBD89_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO7135400.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            3085..3309
                     /locus_tag="VBD89_000856"
     CDS             3085..3309
                     /locus_tag="VBD89_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7135401.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            3464..4423
                     /gene="fabK"
                     /locus_tag="VBD89_000857"
     CDS             3464..4423
                     /gene="fabK"
                     /locus_tag="VBD89_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO7135402.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            4443..5369
                     /gene="fabD"
                     /locus_tag="VBD89_000858"
     CDS             4443..5369
                     /gene="fabD"
                     /locus_tag="VBD89_000858"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO7135403.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYCLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVSRRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            5378..6112
                     /gene="fabG"
                     /locus_tag="VBD89_000859"
     CDS             5378..6112
                     /gene="fabG"
                     /locus_tag="VBD89_000859"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO7135404.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            6128..7360
                     /gene="fabF"
                     /locus_tag="VBD89_000860"
     CDS             6128..7360
                     /gene="fabF"
                     /locus_tag="VBD89_000860"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO7135405.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDVDRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            7326..7862
                     /locus_tag="VBD89_000861"
     CDS             7326..7862
                     /locus_tag="VBD89_000861"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO7135406.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            7859..8281
                     /gene="fabZ"
                     /locus_tag="VBD89_000862"
     CDS             7859..8281
                     /gene="fabZ"
                     /locus_tag="VBD89_000862"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7135407.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            8319..9689
                     /gene="accC"
                     /locus_tag="VBD89_000863"
     CDS             8319..9689
                     /gene="accC"
                     /locus_tag="VBD89_000863"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO7135408.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIITNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            9698..10573
                     /locus_tag="VBD89_000864"
     CDS             9698..10573
                     /locus_tag="VBD89_000864"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO7135409.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFIDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            10566..11339
                     /locus_tag="VBD89_000865"
     CDS             10566..11339
                     /locus_tag="VBD89_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO7135410.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            11807..12439
                     /locus_tag="VBD89_000866"
     CDS             11807..12439
                     /locus_tag="VBD89_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO7135411.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(12485..13762)
                     /gene="serS"
                     /locus_tag="VBD89_000867"
     CDS             complement(12485..13762)
                     /gene="serS"
                     /locus_tag="VBD89_000867"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO7135412.1"
                     /translation="MLDLKRIRTDFDAVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     SEEAKAQRNVASAAIAQAKRNKENADEQITAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFEAKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            14061..15122
                     /locus_tag="VBD89_000868"
     CDS             14061..15122
                     /locus_tag="VBD89_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEO7135413.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWTQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSIGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            complement(15160..15522)
                     /locus_tag="VBD89_000869"
     CDS             complement(15160..15522)
                     /locus_tag="VBD89_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO7135414.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
     gene            complement(15641..16552)
                     /locus_tag="VBD89_000870"
     CDS             complement(15641..16552)
                     /locus_tag="VBD89_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7135415.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            complement(16567..17379)
                     /locus_tag="VBD89_000871"
     CDS             complement(16567..17379)
                     /locus_tag="VBD89_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO7135416.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            complement(17412..18422)
                     /gene="manX"
                     /locus_tag="VBD89_000872"
     CDS             complement(17412..18422)
                     /gene="manX"
                     /locus_tag="VBD89_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO7135417.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            complement(18724..19536)
                     /locus_tag="VBD89_000873"
     CDS             complement(18724..19536)
                     /locus_tag="VBD89_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7135418.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            19625..20209
                     /locus_tag="VBD89_000874"
     CDS             19625..20209
                     /locus_tag="VBD89_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135419.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            20299..20910
                     /locus_tag="VBD89_000875"
     CDS             20299..20910
                     /locus_tag="VBD89_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO7135420.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSI
                     LFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKP
                     QVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGKKLEK"
     gene            21001..22422
                     /locus_tag="VBD89_000876"
     CDS             21001..22422
                     /locus_tag="VBD89_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO7135421.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            22505..23014
                     /gene="tsaE"
                     /locus_tag="VBD89_000877"
     CDS             22505..23014
                     /gene="tsaE"
                     /locus_tag="VBD89_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO7135422.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            22968..23528
                     /locus_tag="VBD89_000878"
     CDS             22968..23528
                     /locus_tag="VBD89_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7135423.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            23537..24844
                     /locus_tag="VBD89_000879"
     CDS             23537..24844
                     /locus_tag="VBD89_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7135424.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(24908..25204)
                     /locus_tag="VBD89_000880"
     CDS             complement(24908..25204)
                     /locus_tag="VBD89_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135425.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(25201..25620)
                     /locus_tag="VBD89_000881"
     CDS             complement(25201..25620)
                     /locus_tag="VBD89_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO7135426.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            25956..26459
                     /locus_tag="VBD89_000882"
     CDS             25956..26459
                     /locus_tag="VBD89_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135427.1"
                     /translation="MKLNKIILSTAALTALFLGYNSVTADTYNNYQPHRSNNMDLTEE
                     YNYNNQIELQERIKNLNIPFKEKLALLRKVKSRQDSYVLDTLRKEVENKNSEYSELEQ
                     EYQRISALINAKFPQGKSSLAEKLHKELIWDLDGVKYDEGISKRTTLKKLEGKLNEYT
                     KKYSYLF"
     gene            complement(26511..26697)
                     /locus_tag="VBD89_000883"
                     /pseudo
     CDS             complement(26511..26697)
                     /locus_tag="VBD89_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26769..27494
                     /locus_tag="VBD89_000884"
     CDS             26769..27494
                     /locus_tag="VBD89_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135428.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            27496..>27897
                     /locus_tag="VBD89_000885"
     CDS             27496..>27897
                     /locus_tag="VBD89_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135429.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGEIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVVQTLFLVLISPILIKLGLSVFMIT"
BASE COUNT         8940 a         4289 c         5593 g         9075 t
ORIGIN      
        1 gaattatttt attataaaag gcgagagcgt tttttaaaac aacatggaat tacaattaac
       61 catttaccaa tgaatttttt tattggagga aatatgaagc aggtttggaa atctgtttta
      121 ggtgatcaat atgatacctg ggatattgat aagctccaac aagattatag tcgctataaa
      181 gaggataatc ccttgccata caaagattta atttttcagg attgtaaaag agtcattgaa
      241 aagcttcatc acaaaggcta tcttttgggt ctagcttcaa gttctactag acatgatatc
      301 atgttagcat tagaaagttt taatctggat acatatttta aggtaattct gtctggtgag
      361 gaattttcag aaagtaaacc taatccagct atttataata gagcagcaga acttttagat
      421 attcctaaac agcaaattct tattgtagaa gacagtgaga aggggattgc agctggcatt
      481 gcagctggta ttgatgtgtg ggctattgaa gacaaatact ttggaatgaa tcagtcgcag
      541 gctaatgtat tagtgagcga tctgagtcaa ttttttgtga aattagataa ctattaaaaa
      601 cgggaggttt aattccgttt tttttatttt ttatcttgcc atgaaagcta ggtttatgat
      661 aaaatacaaa gcatgataat ttgataatca aattattttt tatttttctt tcactcttaa
      721 agagaggaga cgttttgaag ttcgaaaata taatatatgg tattgatggc agcgtggcca
      781 ctattatgct taatcgacca gatatttcaa atggttttaa catcccaatg tgtcaagaaa
      841 ttattgatgc aataaggctt atgtctgaaa ataaagatgt catgttttta gtgattgaag
      901 cacaaggacc aatcttttca atcggaggag atttaaaagt gatgaaagat gctgttgaga
      961 gcgatgatat tagctcatta actaaaattg ctgagttagt taatcagata tcgtatgatc
     1021 tactacagct agagaaacct gttgttatgt gtgtagatgg ggctgtagct ggtgccgcag
     1081 ctaatattgc attagcagct gattttgtta tcgcaagcaa gaagagtaaa tttatccagg
     1141 cttttgtggg tgtcggttta gctccagatg caggaggttt attgttactt agtaaatcaa
     1201 tcggtataac tcgtgcagtg caactagctt taactggaga aagcttatct gctgaaaaag
     1261 ctgaagcttt gggaattgtt tataaacttt gtgaatcaga taaaataggt aaaattaagg
     1321 atcaactatt aaagcgttta agtagacatt ctataaactc atatcaagcc ataaaatcat
     1381 tagcttggga agctgctttt aaagattggg aacaatataa aaagcttgaa ttacagttac
     1441 aagaatcatt agcctttaag caagatttta aagagggtgt tcgggcacac gctgatagaa
     1501 ggcgtcctaa ttttttaggc aaatagtttg aaaatcaaat tatttaagat taaagtcttg
     1561 caaataagtt tgaactttga tataatttga ctatcaaagt ttaatgtgag gtggtgatat
     1621 cttggaatac gatcagatta atagttattt agttgacatt tttaatagaa tcatgattat
     1681 tgaggaaatg agtttaaaga ctagccaatt tagcgatgta tctttaaaag aaatgcatac
     1741 tattgaaatt attgggaaac attctgaggt aactcctagc gatgtcgctc gagaattaat
     1801 gttaactttg ggaactgtaa caaccagcct taataagtta gaaaaaaaag gctacattga
     1861 gcgtaaacgg tcaagtattg atcgtcgagt tgttcacttg agtcttacta agagaggtag
     1921 gttattagat cgattgcata gtaaatttca taaatctatg gtatctcata ttattgaaga
     1981 cctaggcgaa gaagacataa aaatgttgac gagcgcttta gggaatcttc ataaattttt
     2041 ggaggactta gtctagtggt ttttgctaaa attagtcaac ttgcacatta tgcaccttct
     2101 caaatcatta aaaatgagga tttatcatta atcatggata ccagtgatga ctggatttcg
     2161 tcacgtactg gtataaaaca acgtcatatt agtaaaaatg aaacaacagc tgatttggca
     2221 aataaggtcg cagaacaact tattgaaaaa tccggttatt ccgctagtca aattgacttt
     2281 attattgttg caacgatgac accagactct atgatgcctt ctactgccgc aagagtgcaa
     2341 gctcatattg gagcgagtaa tgcttttgcc tttgacttga gtgcagcatg tagtggtttt
     2401 gtttttgcct tgtctaccgc cgaaaaattg atatcatcag gtagctatca aaaaggttta
     2461 gtaataggag cagaaactgt ttctaaagta ttagactgga ctgatagagg gacagcagtt
     2521 ttatttggag atggagctgg aggtgtcttg ctagaagctt ctaaggaaaa acattttctt
     2581 gcagagtctc tcaatacaga tggtagtcgt cagggacttc aatctagtca agtcgggtta
     2641 aattcacctt tctcagatga agtacttgat gataaatttt tgaaaatgga tggtagagca
     2701 atctttgatt ttgctatcaa agaagtttca aaaagtatca atcatcttat agaaacatcc
     2761 catttggaaa aagaagatat tgattatctt ttcttacacc aagcgaatcg aaggatttta
     2821 gataagatgt ctcgaaaaat tgatatagca cgtgataaat ttccagagaa tatgatggat
     2881 tatggtaata ctagtgcagc tagtatccct attttacttt cagaatctta tgagaacggt
     2941 ttgttgaaac tagatggtaa ccaaacaatc ctcttatcgg gtttcggtgg aggtttgaca
     3001 tggggaagtc taattgttaa aatttagtta tatatagtgc atagcacatt tataaaagat
     3061 tatttaaaat ttaaggagct aaaaatggca gtatttgaaa aagtacaaga aattattgtt
     3121 gaagaacttg gtaaagatgc agaagaagta acgttgaaca ctacttttga cgatcttgat
     3181 gctgattcgc ttgatgtttt ccaagtgatt tcagaaattg aagatgcatt tgatatccaa
     3241 attgagacag aagaaggact aaatactgtt ggtgatttag ttgcttatgt tgaagaaaaa
     3301 gtaaaataat taagtcgtaa gaaaaaaaga agcaagtgat aataaaagat attcttacca
     3361 aatcacttat tagagtgagt attcaatcaa tcgttgatcg gtactcactc ttcttcttat
     3421 caatatagtt tgattgtcaa attattaatt ttaggaaaga aaaatgaaaa cacgtataac
     3481 agaattacta aatataaaat atccaatttt tcaagggggt atggcgtggg tcgctgatgg
     3541 agatcttgca ggagctgttt ctaaggcagg tggtctaggt attattggtg gaggaaatgc
     3601 ccctaaagag gttgtcaaag caaatattga taaaataaaa tctatgactg ataaaccctt
     3661 tggtgtgaat attatgcttt tatcaccttt tgtagatgat attgttgatt tggttattga
     3721 agaaggtgta aaagttgtaa cgactggtgc aggtaatcct ggtaagtaca tggaacgctt
     3781 tcatgaagca ggtataacag taatccctgt tgttcctagt gttgcattag caaaacgtat
     3841 ggagaaatta ggggctgatg ctatcattac agaaggaatg gaagcgggtg gtcacatcgg
     3901 gaagctgacc acgatgacat tagtgcgaca ggttgttgat gctgttacaa ttccagtcat
     3961 tgccgcaggt ggtatagcag atgggcgtgg ggctgctgcg ggtttcatgt taggagctga
     4021 tgctgttcag gtaggaactc gttttgttgt tgctaaagaa tctaatgctc accctaatta
     4081 caaagcaaaa attttaaaag caaaagatat cgacactgct gtttctgctc aggtagtagg
     4141 tcatccagtt cgtgccttaa aaaataaact agtaacaacc tatagccaag cagaaaaaga
     4201 ttatttagct ggccgtattt caatcaatga gattgaagaa ttaggagcgg gagctcttcg
     4261 caatgcagtt gttgatgggg atgttattaa tggttcagtt atggcaggac aaattgcagg
     4321 attaattaaa tcagaagaga cttgtcaaga gattcttgaa gatatttatt caggagcgcg
     4381 tcaagttatt ctttctgagg caagccgctg gtcagattta taaatacaaa taaggagaca
     4441 ctatgaataa agttgcattt ctatttgctg gtcaaggtgc tcaaaagtta ggaatggcta
     4501 gagatttata tgaaactttc ccgattgtta aagaaacttt tgataaggca agtcatgttt
     4561 taggatatga tttgagagaa ttgattgata aagacttaga caaattaaat cagacgaaat
     4621 atacgcaacc agctatttta acgacttcga ctgctattta ttgtttaatt cttaaagaaa
     4681 ttggacttag accagatatg gttgcaggac tttctttggg agaatatagt gctttagttg
     4741 cttcaggtgc tataagattt gaagatgctg tagtacttgt ttctagacga ggccaactaa
     4801 tggaagcagc agcacctgct ggtagtggca aaatggtagc ggttttaaat gctgatcgtc
     4861 aaataattga agatgcttgt aagaaggctt ctcagtttgg tattgttagt cctgcaaatt
     4921 ataatactcc aaagcaaatt gttatagggg gagagtcaat cgctgtaaat gcagctgtag
     4981 aagaactaaa acaacaagga gtaaaacgtt taattcctct gaatgtttca ggtccttttc
     5041 acactgcttt attgaaacct gcaagtcaga aattgagcga tgttttagat aaagttcatt
     5101 tttcagtttc tgaaatccct gtaattggaa atacagaggc gcagataatg aaaaaagacg
     5161 atatcaaatc tttattggca cgccaagtta tggaacctgt acgctttgat gagtctattg
     5221 aaaccatgaa aaaaatggga atgacacagg ttgttgaaat tggtccaggg aaggtactct
     5281 caggtttctt gaaaaaaata gatagctcat tatcagtata ttctgttgaa gataaaattg
     5341 gatttaataa tcttaaggaa ttaaactagg ggaaaatatg caattaaaag ataagaacat
     5401 ttttattacg ggttcaagta gaggaatcgg ccttgcaatt gcccatcaat ttgcacaatt
     5461 aggtgctaat attgttttaa atggacgttc tgaaatatca gaagacttaa tagctgaatt
     5521 tgctgattat ggtgtaaaag ttattgctat ttcaggagat gtttcttctt ttgaagatgc
     5581 taatcgcatg attaaagaag ctattgcaag tctaggtagc gtggatgtgc ttgttaacaa
     5641 cgctggtatt actaatgata aattgatgct taaaatgact gttgaagatt ttgagagcgt
     5701 tcttaaaatt aatttaacag gtgcatttaa tatgacacaa tcagtcttga aaccgatgac
     5761 aaaagcgcgt caaggtgcta ttattaatat ctctagcgtc gttggattaa ctggaaatgt
     5821 agggcaagct aattacgcag cctctaaagc aggtttaatt ggctttacaa aatcagttgc
     5881 cagagaagta gcagcacgag gcattcgtgt caatgccatt gctccaggct ttatcgaatc
     5941 tgatatgaca gatgttatcc cagaaaaaat gcaagaagct attttagctc aaattccaat
     6001 gaaacgtatt ggaaaaggta aagaagtagc gcaggtagcg tctttcttag cagaacaaga
     6061 ataccttaca ggacaagtta ttgccattga tggcggtatg acgatgcaat aataaagagg
     6121 aaataatatg acattacagc gagtagtagt aacaggttac ggtgtaacct cacctattgg
     6181 aaacacacca gaagaatttt ggaatagcct taaagaagga aatgttggta ttggacctat
     6241 cactaaattt gattcaagtg atttcatggt caaaaatgct gctgaaattc atgattttcc
     6301 atttgataaa tattttgtca aaaaagatct gaacagattt gatatgtatt ccctttatgc
     6361 cttatatgct tcaagtgaag ctattcaaca tgctaattta aatttagatg aaattgatgt
     6421 agatcgattt ggtgtcatcg ttgcatcagg tattggaggg attcaagaga ttgaagaaca
     6481 agtgattcgt cttcatgaaa aagggccaaa acgcgtaaaa ccaatgacgc ttccaaaagc
     6541 tctaccaaat atggcagctg gaaatgttgc gatgaggtta ggcgctcatg gtgtatgtaa
     6601 atcaattaat acagcttgtg cttcttctaa tgatgctatt ggagatgctt tccgtaatat
     6661 aaaatttggt attcaagata ttatggtagt cggtggtgca gaagctgcta ttacaaaatt
     6721 tgccattgct ggattccaaa gtcttacagc attatcaaca actgaagatc catcaagagc
     6781 atctattcca tttgataaag atcgcaatgg atttatcatg ggagaaggtt ctggaatgtt
     6841 ggtcttagaa agtttagaac acgctgaaaa acgaggagct actattcttg cagaagttgt
     6901 tggttatggt aacacttgtg acgcatatca tatgacttca ccacatccag aaggtttagg
     6961 agccactaaa gctattcaac ttgcactggc tgaagctaat attaaacctg aagaagttaa
     7021 ctatgtaaat gctcatggaa cttcaacacc tgctaacgaa aaaggagaga gtcaagctgt
     7081 tgttgctgca cttgggacag acgttccagt ttcttcgact aaatctttta ctggtcatct
     7141 tttgggtgcc gcaggagctg ttgaggcaat tgcaactatt gaagcgatac gtcatagtta
     7201 tgttccaatg actgctggaa caacagaatt atcagaagat attacagcga atgttatttt
     7261 tggtcaaggg caagacgctg atattcgtta tgccatttca aatacatttg gttttggagg
     7321 acacaatgca gttctggcct tcaaacgctg ggaggattaa attggatatt caagagatta
     7381 aggatttaat gactcaattt gatgagtcaa gtctgagaga attttcattc aaaacaagtg
     7441 acggtgagct gtcttttagt aaaaatgagg gaaaagctcc acttgtacca actatgtctc
     7501 ctatgagtca tcaacctgaa gcgactccaa ctatagcaac tcctgtctct aatgaagcag
     7561 gtgagcaaac gaaacaagct actgaagtag tttcagaaat cccagagtcg actgtgactg
     7621 ttgcagaagg tgacgttgtc gaaagtcctt tagttggggt tgcttattta gcttctggac
     7681 cagataaacc taactttgtt tcagtaggtg atagcgtgaa gaagggacaa actttgatga
     7741 ttattgaggc aatgaaggtg atgaatgaag tgcctgcccc tcatgatggt gtagttactg
     7801 aaattcttgt agccaatgaa gaagttattg aatttggtaa aggattggta cgaatcaaat
     7861 gattgatata aaagaaattc gtgaggcttt acctcaccgc tatccaatgt tattagtgga
     7921 tcgtgtttta gaggtatcag aagatgagat agtcgctatc aaaaatgtta gtatcaatga
     7981 gccttttttc aatggtcatt ttccagaata tcctgtaatg ccaggcgttt taattatgga
     8041 agctttggct caaactgcag gtgttttaga attatctaaa gaagaaaaca aaggaaagct
     8101 tgttttttat gcaggtatgg ataaagtgaa atttaaaaag caagttgttc caggagatca
     8161 attagttatg actgctaagt ttgtcaaacg tagaggaact atagccgttg ttgaagcaat
     8221 agcagaagtt gatgggaaat tagctgccag tggaacgtta acatttgcaa taggtaattg
     8281 aagatagtat tgaatttaga gggagagaag catcaaaaat gtttaagaaa atattgattg
     8341 ctaatcgcgg agaaattgca gtacgtatca ttcgtgcagc ccgtgaaatg ggaatttcga
     8401 cagttgctat ctattcggag gctgacaagg aatcattgca cacaatttta gcagatgaag
     8461 ctatttgtgt tggaccagcc aaatcagcag aatcttattt aaacgttaat gctatattat
     8521 ctgctgctat tgtgactgga gcggaagctg ttcatccagg ttttgggttt ttgagtgaaa
     8581 attctaaatt tgctactatg tgtgaagaga tgaacttaaa attcattggt ccatctggag
     8641 aagttatgga taaaatgggt gataaaatca atgctcgtgc agagatgata aaagctgatg
     8701 ttccggttat cccaggatca gatggtcaag ttacaagtgt agaagaagct gtatcaattg
     8761 ctgaggaaat cggctatcca ttgatgttaa aagcctcagc cggtggcggt ggaaaaggaa
     8821 ttaggaaggt caaatcagca gatgaattga agcctgcttt tgagtctgct tcacaggagg
     8881 ctttggctgc gttcggtaat ggtgctatgt atattgaaaa agttatatac ccagctagac
     8941 atattgaagt acaaatactt ggagatagct ttggtaagat agttcaccta ggagaacgtg
     9001 attgttctct gcagcgtaac aatcagaagg tattggaaga aagtccgtct gtggcaatag
     9061 gtaacactct gcgtcaacaa attggtgaag cagctgttcg tgcagctgag gctgtatctt
     9121 atgaaaatgc aggaacaatt gagtttttat tagatgagaa tagcggacag ttctatttca
     9181 tggaaatgaa tactcgtgtt caagtagaac accctgttac agaatttgta acgggtgttg
     9241 acatcgttaa agagcagatt cgtatcgctg ctggaattcc tttatcagtt tctcaaaatg
     9301 atattaagtt aactggtcat gcgattgaat gtcgtattaa cgcagagaat ccacaattta
     9361 attttgcgcc atgtccaggt accattaatg ggttacatct acctgctggc ggtatgggct
     9421 tacgtgtcga ttcagcggtt tacacaggct acactattcc tccttattat gatagtatga
     9481 ttgcgaaagt aattgttcac ggagaaaatc gttttgatgc acttatgaaa atgcaacgtg
     9541 ccttatatga gcttgaaatt gatggtatta taacaaatac agaatttcaa atggatctca
     9601 tttctgataa aaaagtcttg gctggagatt atgatacttc attcctgatg gaagactttt
     9661 taccaaggta ttatggtaac aatcaatagg agggaagatg gctttattta gtaaaaagga
     9721 taaatatatc cgtattagtc caaacaaagc attagggtct tctgataaac gtagtcttcc
     9781 tgaggttcct gatgaactct ttgctaagtg tccttcttgt aaacatatga tttatcagaa
     9841 agatttagga ttagcaaaga tttgtccagc ttgttcttac aattttagaa tttcagcgca
     9901 agaacgtctg ttgctaacag ttgacgaaga tagttttgag gaattattta cagggattga
     9961 aactaaggat cctttgaatt ttccaaacta ccgtgaaaaa ctggctgcta cacgtcaaaa
    10021 gactaactta gatgaagcgg tggtgacagg tttagctaaa ataaaaggac aaactacggc
    10081 cttagctatt atggattctc attttataat ggcgtctatg ggaacggttg taggcgaaaa
    10141 gttgacaaga ttatttgagc tagcaactga aaagaaatta cctattgtta tttttacagc
    10201 gtcagggggc gccagaatgc aagaaggtat tatgagtttg atgcaaatgg caaaagtatc
    10261 ggcagcagtt aaacgccatt ctaaccaggg attgttttat ttaacaattt taacagaccc
    10321 aacaaccggc ggcgttactg cgagttttgc tatggaaggt gatattattc tggctgagcc
    10381 gcaagcgttg gttggttttg ctggtcgtcg tgttatcgaa acaacggtga gagaagattt
    10441 acctgaaggc tttcaaaagg cagagttttt gttagaacat ggttttattg atgctattat
    10501 aaatcgaaca gaattacgcg actgcattgc tcaactaatt gctttccatg gaaatgaggt
    10561 tggtcatgaa taatgatgtg acaagaattt taaaagatgc gcgtgatcaa gggaggctaa
    10621 ctgctcttga ctacgctgaa ttgatttttg ataattttat ggaacttcat ggtgatcgcc
    10681 agttcgcaga tgacaaatca attataggag gtttggggta cttggcaggg cgtcctgtga
    10741 cgattgtcgg tattcaaaaa ggtaagaacc ttcaagataa tttagaccgt cattttggtc
    10801 aaccacatcc tgaaggttat cgtaaggctt tacgtttaat gaaacaagct gagaagtttg
    10861 gaagacctgt tataaccttc atcaatactg ctggagcata tcctggggta ggtgctgagg
    10921 agcgaggtca gggtgaagca attgctagaa acctcttaga aatgagtgat ttaaaagtcc
    10981 ctattatagc tattattatt ggtgaagggg gttctggagg agcattagct ttagctgttg
    11041 ccgataaggt ttggatgttg gaacatactg tttattctat tttaagtcct gagggctttg
    11101 cctctatttt atggaaagat ggtacaagaa caacagaagc agcacaatta atgaagatga
    11161 ctgctggaga actttaccat atggaagttg tagacaaagt aatccctgaa catggttatt
    11221 tttcaagtga gattgtcgat atgattaaga caagtttaat ttcagagtta gaaatgctct
    11281 cacaattatc tcttgaggat ttattggagc aacgttacca acgttttaga aaatattaat
    11341 agaaaaaggc tgagttattt actcagccta tatttagttt ttcaaggtat tatcatagtt
    11401 atagtttcat attttgctat aattgataaa aaggagatat gtcaatgaca ctagcattag
    11461 ccaattttta gattgtagtc taatccaaaa ttttcagaaa gagtcatttc gaacacttta
    11521 ttctaagtac cattatttag gctctcctta acttgagact ctatctgtta ttgaagagaa
    11581 attttctaga aaaaatagct attgttagat tttagaaggc tatgaaaaga ttggttttta
    11641 aagagttcta ttaagtgatg aagaaagtaa aacttggtta gttataattg cgattagact
    11701 ttgtttctag caacgagact tcgctataca tactttaaaa gaacttgaaa gtatgtatag
    11761 atatataatc gtgaaaactt aactattgtt tacatggaga aaaagaatgt atcaagaatt
    11821 aaatgattat ttagaattcc aatcaatgaa gtatattatg cctgagaagg caggcccttt
    11881 aaaggacgat atgcaaactt ttaaggcaaa gggccaactt gctaggaata gctttacaga
    11941 gttatcaaga gccttagaac aacgtatgga tggctttaaa atgcaaagag tgagtaattg
    12001 ggctaatcaa gctcaagtag ggcgtccaca tttttgggtt tattatcgaa aggatacaga
    12061 ccaattagat gatgtggctg ttgcgctaag ggtttatggt gtaaaagata gttttggggt
    12121 tagtcttgag gtaagctttg tagagaggca aaaatctgat aagacactcg agaagcaggc
    12181 tagagtgtta tcgattccga ttgcctctcc attgtatttt atggtccaaa ggcaaggtga
    12241 aactcatcga gaggaaggca atgaggaaaa tcgtcaacgt ttaatgcaag aaattaagtc
    12301 agggaaggta aggaaagtat tggtaaaata tgatgttctt ttgactgaaa accagagctt
    12361 agaaaatatt ctgcaacggt tgttagaagg atttgagaaa gtgctacctt actaccaagt
    12421 ttgtcaaact attagctaag ttcatggaaa aaatgagttg gtataccaac tcattttaga
    12481 gtgcctaatt tggtttaata atatcgatat tacccatata tggacgaaga acttctggga
    12541 tagtcacaga accatcttca ttttgataat tttcgaggat tgctgctaca gtacgtccaa
    12601 ctgcaagtcc tgaaccattt aaagtatgaa gcaacctaac tttgccatca acttcgtcac
    12661 ggtaacgaat ttgagcacga cgtgcttgga aatcttcagt attagaacat gaagaaattt
    12721 ctcgataagt attttgagcg ggaatccata cctctaagtc atacgtttta gctgctgaga
    12781 atcccatatc tcctgtacac aaggtaatga cacggtatgg gagattaagt ttttgaagaa
    12841 tattttcagc atttgctgtc attttttcta attcttggta cgattcctca ggcttggcaa
    12901 atttcaccat ttctacttta tggaattgat gaaggcgaat taatccacgt gtgtcacgtc
    12961 ctgcagagcc tgcttctgaa cggaatgatg gactcatggc agtaaagtat ataggtaatt
    13021 ctttcccatc gataatttca tcacgataat agtttgttaa aggaacttca gctgttggga
    13081 taagtacgaa tgggctatct gctaattcga aagtatcttc cttgaacttt ggatattgtc
    13141 ccgtaccgaa cattgaatcg tggttaacca tataaggagg gatcacttca gtgtaccctt
    13201 ccttagcgtg ttcatccagc atgaagctgt agatagcacg ctctaaacga gcacctagac
    13261 ctttgtagaa gaggaagcga gaaccagtaa cttttgcacc acgttcccaa tcgagaattc
    13321 caaggctttc ccctaaatcc cagtgagctt tggcctcaaa atcaaattca cgaggagttc
    13381 cccagcgacg aacttcaaca ttttcatcct cgtctgctcc aagcggcaca tcatcagctg
    13441 gtgtattagg aagtacggtc accattgatt gtaaattagc atccacatct gccaattccg
    13501 catcaattgc tttgatatca gcggaaagtg tttgcatagc tgtgatttgc tcgtctgcat
    13561 tctctttatt tcgttttgct tgagcaatgg cagcagaagc aacattacgt tgcgctttcg
    13621 cctcttcaga tttgataagt aattcacggc gttttatatc aagttcttta agggttgtta
    13681 atgtttcttg gtcaacacca cgcgtggcca attttttagc gacagcatca aaatctgtac
    13741 gaatacgttt taagtctaac atataggaat ctcctttata ctaaaaaaaa caccctgtca
    13801 tgctggagcg caaaggcgcg gttccatcca gcttcacaga agtgcacttg attgattgat
    13861 taaattattt cccataaatg gaacacggta gaatttcata aaacaatctt atctgctcgc
    13921 aacgaccgca gactctctga aaagaactat tatactactt atccgttaag gttattatat
    13981 ccaaaaataa gtaaaatgta aaccttaact ttataagcct gctctttgtt ttagtgaaat
    14041 agctatgcta aaatatgatt atggaaaagt atacaagtat taaacaatca ggtcgtcatt
    14101 acggcttaga ccttttaagg attatttcca tgttcatgat tgttattacg catgttcttg
    14161 gaaaaggtgg tctgagatct tcggttgagg gacacgcaga ctcctatttt atagtaacgt
    14221 ggattatcca agtcttagtt tatggggccg ttaactgtta cgcactaata agtggttatg
    14281 tgggaattaa ttcgcgttat cgttactcaa aattactgtc tatctggaca caggtttttt
    14341 tctacacctt tacaataaca gcactttttg caatcacagg tcatgaagtg accttgctta
    14401 attggagaga tgcttttttc cctattgtca gtgggcagta ttggtacatt actgcttact
    14461 ttggtctatt ggtttttatg cctgttatta ataatggatt aaatgccttg actgataaac
    14521 aattaaaaca attggttttg ttaatgttta ttattttttc aattttacca gctgttttaa
    14581 acaatcgtgt tccagaattt tcattgagca agggttttga aatgacttgg ctactgattt
    14641 tatatattat tggtgcctat ttgaaacgta ttgatttaaa tatatttaaa acatcatact
    14701 tacttatagt ttatctatta tcattggttg ctacttatgt catgaagttt agtattggtg
    14761 atatttggta ttggtatgtt tcgcctactt taactttagg ggcggtaagt ttgtttatat
    14821 tatttgctag agcaagcatt aagccctcgg gattcttaaa gaaaataatt gttgtattag
    14881 ccccttcaac cttgggagtt tatctctgtc atctccaccc actaatagta aaatattttg
    14941 taagagattt tgcagagacg tttgtttatg agtccattta tctttaccca tttttaattt
    15001 taggagcagg gattttaatt tatttgttgg cttttattgt agaacgtatt agattacgaa
    15061 tatttaaagc aatccatttt tctaactttt tgagatggtt tgatgacaag ttcccatatt
    15121 aatattagag ttaaaaaata agatagacta tagtctatct tattttttaa acttgaatat
    15181 ttttaaaatg gtctggatga gggtaggaag cttaacaact ttatcgtctc caatatgtcg
    15241 tctcgcatgc tttaatatcg ttccagagtc tttagaggca aataaaaatt ttccttggtc
    15301 tgtaaagacc tcaaagtgac gactaatttt ttttcgacta acattagctc caatttgatt
    15361 gattgatgtc catggaattt gaatatagtc gttgacattt ttatcgttat agaattctaa
    15421 tgcctggtca ccaactaaaa attttccaac tttattcccc atacctaaat acgaaactcc
    15481 tgttgtttga aattcaacag ttttgtttaa tgattgtgcc atgactaatc cctttttaat
    15541 ttatagtagt ttgattatat cataatagtg ggactctaag tggatttttg gtaataaaat
    15601 ataagagatg gaatactatt gtaaccatct cttagtttta ttacatcaca cctgcaaggc
    15661 gagctagaat accaacgata aacaaaccga tgataattgt aattggtgat actttcttct
    15721 taagtaacca catacagaag aatgtaagaa gtaatccagc taaaccagga ataagtgagt
    15781 caagttgtcc ttgaagtgtt tgtggttgca ttttatcaag gcttaagcca ctctgaactt
    15841 gtcctaggat atcatgcaat tgttgtccac taacatggtc tttagggaaa ttgatgtatg
    15901 ccccttctga caatgtcttt ttaggaaggt ctacagtaaa gttaattgct acccaacgct
    15961 taacaaggac agcaagaatg aacataccaa ggattgaagc acctttagta atatcttgga
    16021 ggataccgcc agacatatct ttagtaattt ctttacctga tttgtaacct aactcttgtg
    16081 tataccataa gaatgacata cgaatgaggt tccaaccaac aaagaagatg attggtccga
    16141 ggatattacc agcagaggca agtgaagcac caagagcacc taagatagga cgtacagtaa
    16201 accagaatac tggatcacca ataccagcaa gaggtcccat cataccgatt ttaacccctt
    16261 gaattgcttt gtcttcgaca ggtgtaccgc ttgctttttc ttcctcaaga gcaagggtta
    16321 caccgataat tggagcagca acgtatggat gtgtgttaaa gaattccata tggcgctcaa
    16381 gcgcagcagc acgatcttct ttagtagtgt aaagtttttt caatgctggg ataagagcgt
    16441 aggcccaccc catattttgc atacgctcat agttccaaga accttgaagg aattgtgaac
    16501 gccaccaaac tttttgacga tctgattttg ataatttaat ttgttcagtc attgtttagc
    16561 tcctttctag tagtcctcta agatatcgcc gataggatcg ttagaacctg cgttagaagt
    16621 acctttgctt ccacccattt ttgataaact gatgtagatg agggcgatag caacaccaat
    16681 agtacccatt gcaataagag ttaactggtt caaagctgct agagcaaatc caagagcaaa
    16741 gaaaggccaa acttcgcgag tagccatcat attgataacc atagcgtaac caacggcaac
    16801 aaccatggca ccaccaactt gcataccacc atttaaccaa tcaggcattg cttcaagaat
    16861 actttgtact gaagaagatg gaatagctaa aagaagcgca gctgggaatg caatacgtaa
    16921 tccttggaga aggagggcaa taaagtgaaa acgttcaatt gctgcgaagt ttccttcaga
    16981 agcagctttg tcaccagcgt gaacaagtgc agttgagatt gtacgaacaa tcattgtaag
    17041 gaaaagtcca gcaatagcta gaggtacagc agtagagaag gcaaagttga tacctttttg
    17101 agtgaagtcg ccacttttaa ccaaaataat agcagcagca acagaggcaa gtgcagcgtc
    17161 tggtgctacg gcagctccga tatttgccca agcaagagca atcatttgga gactaccacc
    17221 aagaataata cccgcttcga ggtgaccagt tacaagaccg attaaagtac aagcaacaag
    17281 aggttgatgg atttggaatt ggtcgaggat accttcaaga ccagcaaaga aggcaacgat
    17341 aacgaccaaa atcattgaga taattgaaat atccgccatt ttgatatcct ttcgttttca
    17401 gagataataa gttattttac gtttgctttg ttgattaaat caaacaaatt ctttttagcg
    17461 tcatttggta ctttacgtac atcaaatgat acacctaagt cacgtaattt ttcaaaggca
    17521 gcaacatcgt ctttatccat tgaaagaacg ttgtttacca ttgttttacc agttgagtga
    17581 gccattgaac caacgttcaa ttcagggatt tccacaccac cttcaactgc acggagtgca
    17641 tcttgtacag tttcaaataa gatcaaggca cgtgtgtttc cgaaacgtgg gtctttagct
    17701 acttcaatta atttactaat tgggacaacg tttgctttga ctcctccagg agcagcttgc
    17761 ttaatcaatt gtttacgaag ttcatcttta gaaacttcat cagaagcaac gataatacgg
    17821 tttgcttttg atgctggagt ccatgcagtt gcaacttgac catgtaaaag acgtgtgtca
    17881 atacgagcaa gattaatttt aagcttgcca tctccaataa cagttccttc agggatagat
    17941 ccactttgtt taacttccgc aggaacatct gcaggaacac ctgctacagg agtagcttct
    18001 acaacaggat taagttcttc tggaagggct ttgatacctt ccttagactc tttaataata
    18061 tttgcggcaa cttgttcaac acctgcatta gcgtccatca tacgctcagt ataggcttgg
    18121 ataagcattg gcaaattgag accagtgatg atagccattt tacgttcagg attttctccc
    18181 ataacacggc ttgcttggtt aaatggtgac ccactccaaa ggtcagctaa cacaagcacc
    18241 tcatcatcag catcaaattg tgcgatagca ttgttgaagt gtccatacaa atcatctggt
    18301 ccttcatttg gcatgaaagt tacaacttga actttttctt gttcaccaaa gatcattgaa
    18361 cctgattgat gaataccttc agcaaattta ccatggctgg caataataat accgataccc
    18421 attcttgttc ttcctcctta aaaaattttt tcgttgacat aaagaagaat aatctccttc
    18481 caatcgtaaa attggccatt ctttttcatt tcaacgaaat tatagaacag atatatttta
    18541 aaacgttttc agaaaaaaat caatactatt tctaataaaa attaaaaaaa agatattttt
    18601 ttcacaaata ccttttataa tgggggaaga gagtggtaac tttttttgtt aataatatag
    18661 tagaaaacct aaaaaacgat ttctattaga attctgtttc aatagagatc gtttttgaaa
    18721 atactacaat aataattttt ctagagtttt agcaacacca tcttcttcgt ttgtccattg
    18781 gatttgttgg tcggcatatg gaagtagagt tggactagca tttttcatag catatcctgt
    18841 atgtgcaaag gccagcatct cggtgtcatt gtgttcatct ccaaaggcaa tcaaattttc
    18901 ttgtgaaaga ttgagtgatt ttaataaatg tttgagagca taagctttgt taacaccttt
    18961 aggtgaaaat tcgagtatat tcaatggacc accccaagaa tctacttcta gttcgtgatt
    19021 aaaatgttgt cgtaactctt tagctaactc atacttatct ttatgacgtg tttgcatcaa
    19081 tagagcattt ggattgcgtg tgattttagt aacatctaat gccattttat cagtaatttc
    19141 attgacaccg aataattgtg ggttaatttt atcacgatta tccatagtaa tgtaaaaatt
    19201 tttacggtac tcgctagcaa taaaatcggc ttggatagca tctgataaat ttaaggtttc
    19261 aaggagtaat tttttatcaa tcgttgcact atgctcaaac gcccactttt tttcggggat
    19321 atgagttaaa gcaccgttaa aatttatcat tggtgttttt aaatctaggc gtaaataatg
    19381 agcgagtgcc atacggtatg ggcgtccggt tgtaataatt actttgtgtc cttgagcttg
    19441 aacttttcga agtgtatcag cggtgtagtc agaaatagtg ttattattat gtaatagagt
    19501 accgtctaaa tctacggcga taattttctt tgtcatgctg acattatatc aaaaatagat
    19561 ttcaataaaa agagacagta gaaatatgat ataataactg tggaaatatg gaagttggtg
    19621 gattatgaaa aagttaattt ggagtcatct ttttgccata gtcagtattt tagggatatg
    19681 gtattatcat gtaaaaattg atcatttgat ggtaattcta ttggcatcag tcttgtcgtt
    19741 agagatggga ttcattgctt attatagtcg taatattttt attcgtcttt tagcaagttt
    19801 aggcctttta tttttttacc cacagagttt agggattttt cttagtttag tgagcttaga
    19861 gtggtcaaca aaagacattt ggcagtctaa tcactttatg caatatatgg cataccttct
    19921 tgcaattctt tttattatct cttcagcttt actaagtttg aagctattgg taaatgcata
    19981 tgatattagt aaaaggtatc aattagcaac tgttatacct gtaatgcttt ttagtagttt
    20041 tgccttttgc ttagaaaaat caggatcatt aggtttagaa ggaatcattt taaatccgtc
    20101 agcatttttt aatggaatac agactaatat ggctaacctt gattttaaag ccttgtcttg
    20161 ccttttttta gtacaattat tactttgggg aatattaatg gaagactaac cacaacaggg
    20221 ttagtctttt aggtttgaag tttatttaac cttaatttaa taatatgttg tataatgaga
    20281 tattaattgg aggttagaat gaaagtaaaa ctatttgcga tacatttatt ttttattgtt
    20341 atttcttttg ggattaaata ttatcatttg cagggaccaa atttgatttg gaatatgact
    20401 ctagccctaa tagcgttaga ttttgcatac cttactagtt tatttaagaa aaaaattctt
    20461 atcggtcttt ttgcacttgc ttggtttttt ttctatccta atacctttta tatgttgacg
    20521 gatatcattc atatgcattt tgtcggagac gttctttata ataagacaaa tttaatactt
    20581 tatatcttgt atgtatctag tatcttgttt ggttttttat caggtatcga aagtttttct
    20641 gtgattatgc gtaaatttag aattagcaat atatttttaa gatggggaat tattggaatt
    20701 gtatcatttg tttcaagttt tggtattcat attggtcgtt atgcccgtct aaattcatgg
    20761 gatatcttaa cgaagccaca agtggtaatt aacgaacttt tagcggttcc gagtagggat
    20821 tcattccact ttattttagg atttacattt ttacaggtat tgtgtcttat ttttatggat
    20881 tattctgcag ggaaaaagct tgaaaagtga attatttttt gttaatatat attaatcgtt
    20941 cgtaaatata cgaacgatta atatatatta acaaaaaata aaggagaatc cgattttaaa
    21001 atggaaaagt tttttaagct aaaggaacac gggacgacaa ttcggactga aatcactgct
    21061 ggtctaacga ctttctttgc aatgtcgtac attttgtttg taaatccagc tattttatct
    21121 cagactggga tgccagcaca aggagttttc ttagcgacga ttattggtgc cgttgtagct
    21181 acatctgtta tggctttcta tgctaacctt ccctatgcac aagctcctgg gatgggatta
    21241 aatgctttct ttacatatac agttgtcttt gcattgggat atacatggca agaagcctta
    21301 gccatggtat ttatttgtgg tttgatttct ctgattatta ctttaacgaa agttagaaag
    21361 atgattattg agtctatccc aacaacttta aaatcagcca ttacagcagg aatcggaact
    21421 tttttagcat acgttgggat taaaaatgct ggttttttga aattttcaat tgatcctggg
    21481 acgtatgatg ttgttggaaa gggtgctgcg aaaggattgg ctactattac agcaaattct
    21541 tcagcaacgc cagggctagt gtcgtttgat aatccagcaa ttttattatc tttgattggt
    21601 ttaagcatta ccatcttctt tattgtaaaa ggtatccgtg gagggattat tctatcgata
    21661 ttaacaacga ccctcttagg tattttaatg ggagtggtta agttagatgc tattaattgg
    21721 gaagcaacta acctctcggc ctcttttaga gatttgaagc aagtatttgg agttgcatta
    21781 ggagagaaag gattaatttc tttattttct aatccttcac gtctaccaag tgtattaatg
    21841 gccattcttg ctttttcact gactgatatt tttgatacca ttggaacgct cattggtacc
    21901 ggagaaaaag ttggtatctt agcaacgact ggtgataatc atgaatctaa gagtttagac
    21961 aaagcactct attcagactt aattgggaca acttttggtg ctatttgtgg aacatctaac
    22021 gttacaacct atgttgagtc agcagcagga atcggagctg gtggtcgtac aggattgaca
    22081 gccttagtag ttgctggttt gtttgctata tctagttttt ttagtccact agtatctatc
    22141 gttccgagtc aagctactgc accaatattg gttattgttg gtattatgat gctttcaaat
    22201 ttaaaagata tcaaatggga tgatatgtca gaagctatcc cagctttctt tacttctctt
    22261 tttatggggt ttacttatag cattacttat ggtattgcag ctggtttctt gacctataca
    22321 ctagctaaag tcatcaaagg acaagcaaaa gatattcatg tggtactgtg gattttagat
    22381 atattattta ttctaaactt tatcagcttg gctattttgt aatattatag tttgataaat
    22441 aagataaaag gaaatcagat ttgtagcctg gtttcctttt ttcttaagaa aagtgaagaa
    22501 atatatgcta tggggctgtc agaatcagca aactgttgga agacagagat atttctggta
    22561 taatatctct atgttttata ctcaaaatga agaagagctt attgccctag ggcaaaaact
    22621 gggtactgtt ttaaagtcag gagatatcgt attattgact ggtaatttag gtgcaggcaa
    22681 aacaactctt acaaaaggta ttgcaaaggg attagacata aaacaaatga ttaagagccc
    22741 aacttacact attgttagag aatatgaagg gcgtgtacca ctttaccatt tggatgttta
    22801 tcgtattgga gatgatcctg attctattga cttagatgac ttcttgtttg gtcaaggagt
    22861 aacagttatt gaatggggag agttgcttag tgataacctt atcaataact acttagaaat
    22921 tgttattacg aggagcaatc aaggacgtca agttcagtta gaagcttatg ggcatcgcgc
    22981 aagagaaatt attgaggcga ttcaagatgt ctaaacaatt tgctgttgat tttatagaag
    23041 cttcgagaga ggaagcatct gcgcttcttg aatttttaaa tactgttact gaggaaacag
    23101 attttatttt gcatacggtg agcaatcaac tatctttgcc agaaatggaa acgtttattg
    23161 aaaatacctt aataacgaaa aattgcattt gtttgattgc taaattaaaa aataaagtga
    23221 tcggcttgat aacaatcatt agtcagtctg atattgaaat tgaacacatt ggggatcttt
    23281 ttattgctgt tcagaaggat tattgggggt atggtattgg tcatattctc atggaagaag
    23341 caatagaatg ggcgtcagat aacgatatta cacgccgctt agaactatca gtccaaggac
    23401 gtaatgaacg tgctattcac ctctaccaaa aattcagttt tgaaatagat gggcttcaaa
    23461 caagaggtat taaaagagaa aatggagaat ttctagacat ttacagaatg agtaagttga
    23521 tagattagga atatacatga aaatttggaa aaaaataacc ttaatgtttt ctgcaattat
    23581 tttaacaaca gtaattgcat tgggagtcta tgttgcctca gcttataatt tttcgactaa
    23641 tgaattgtct aagactttta aagattttaa gctagctaaa tcaaaaagtc atgctattga
    23701 agaaacaaag ccgttttcaa tactattaat gggtgtggac acaggttcag agcatcgaaa
    23761 atctaagtgg tcaggaaata gcgattctat gatcttagtc actataaatc ctaaaactaa
    23821 taaaacaacg atgacaagct tagaacgtga cgtattgatt aaattgagtg gtcccaaaaa
    23881 taatggacag actggcgtag aagcaaagct aaatgcagcc tatgcttctg gtggtgcgga
    23941 aatggcattg atgactgttc aagacttatt agatattaat gttgattact ttatgcaaat
    24001 taatatgcaa ggattagttg atttggtcaa tgctgttggt ggtataacag taactaataa
    24061 atttgacttt ccaatatcaa ttgctgccaa tgaaccagag tacaaggctg ttgttgaacc
    24121 agggacacat aaaataaatg gagaacaagc acttgtttat tctcgtatgc gctatgatga
    24181 tccagaggga gattatgggc gtcaaaaaag acaacgtgaa gtaattcaaa aagtccttaa
    24241 aaaaatattg gcgttaaata gtattagttc atacaaaaaa attctttccg cagtaagtaa
    24301 taacatgcaa actaatattg agatatcatc aaaaacgatt cctaatttgt tagcttataa
    24361 agattcattg gaacatatta aatcttatca gttgaagggt gaagacgcta ctctatcaga
    24421 tggtggctct tatcaaattt taactaagaa acatctactt gcagttcaaa atagaattaa
    24481 gaaagagctg gataaaaagc gtagtaaaac tctgaagaca agcgcgattc tatatgaaga
    24541 ttactatggt actactgcta gtaatgattc ttctacttat tcatcaacac aagagaataa
    24601 ttataataca acaccttatt cagaagcacc accaagttac agtggtaata ctacttatag
    24661 ttctgagact aatcaaacaa ctcatcaaag ttactataat agtagcactc ctgctagtaa
    24721 ctatagcagt aacactaaca caggtcaggc tgattcaagt ggaagtgtta ataattataa
    24781 cggggctgca acgcctaatc caaacacagg aacgcaacca gtaccaagtc aaactaatcc
    24841 ataaaatata aaaaagagca ttatttgctc tttttatatt tagagtagat atttttatac
    24901 cgtcggctta ttcgtctttc tcttgatatt tagccatgcg ttgattaatt tcttctaaac
    24961 gaggttgaat atctttatta aacacttgga atttatgatt gagttcttga gaaattaatc
    25021 tgattttttc tttttcctga ctaataatag caagatcatt tttgatacgt tctaatttta
    25081 atttagctga atctgtctca ttttttgtat tttgatagga atccttgata gtttttctat
    25141 ttttgtaaag ttcaagcgct cctaaactaa ctaaacctag tgcaattaaa cgacctattt
    25201 tcataattga atctcttttc taatttcttt ggctaatttt gcaagtgcct caaagtcggg
    25261 ttcatgagta gtgtagtgaa ttttgatacc gtctgattca tcaaagcggg gaacaaggtg
    25321 gacgtgtgca tgaaaaacag tttgtccagc aatttcctca ttgttgttaa taatattcat
    25381 tcctttggct tttgtagcag cttggacagc acgcgccact ttaggcaagc gttcaaaggt
    25441 tatctgtgca gttttttcat ccatctctaa cacatttcga acatgttttt taggaataag
    25501 aagggtgtgc ccagtcgtag tttgtgtaat gtcgagaaaa gctaatactt catcatcttc
    25561 ataaactttt gatgaagaga tttctccact gatgatttta caaaaaatac aattatccat
    25621 aactaattcc tttcatgttg aaaaatttat tgggtagcta ttaaggaatg gtaaattttt
    25681 atcgtttgat aggtcataag tcgtacaggt ggataaaagt agcctgtatt gattgtgata
    25741 tctatattta ccatcatact tatcttagca tgacctaaag ggtattgcaa tttttaatga
    25801 tgtttattat tgagtaagta catttctaga attttggaag tatctaaaat aaatttaaat
    25861 ataatgattg acatttttat ctaatgaaat gtataataaa taatataaat aaaataaaaa
    25921 tgataattga gatttatcat agaaggaaaa ctattttgaa attaaataaa atcatattat
    25981 ctactgcagc tcttactgct ctctttttag gatataatag cgttactgcg gatacatata
    26041 ataactatca gccacataga tcaaataata tggatttaac tgaggaatat aactataata
    26101 accagataga acttcaggag cgtataaaaa acctaaatat accttttaaa gaaaaacttg
    26161 ccttactcag aaaggttaaa tcaagacaag attcttatgt attagacaca ttaagaaaag
    26221 aagttgaaaa taaaaacagt gagtattcag aactagagca agaataccaa agaatttcgg
    26281 ctcttattaa tgctaagttt ccgcaaggga aatctagttt agcagaaaaa ttacataaag
    26341 aactaatttg ggacttggat ggtgtcaaat atgatgaggg gatttctaag agaactactc
    26401 taaaaaaatt agaaggtaaa ctcaacgaat atactaaaaa atacagttat ttattctgat
    26461 aattgacttt ggtaatttta tagcctaata ttctgattac tttgcattta ttatattgat
    26521 actaaaaaga cacctttttg aggtgtcttt ttttgtccga tgagtgatgg aggtgctttt
    26581 tgtaaacata atgggagcat ggggtagctt ggcatttcaa ttagatgtaa aagcggacaa
    26641 aataggttta aagttatttt caaaactata agggaattct aagatacaat tatccataag
    26701 aatagacctt ccctttctta ttttgttata atagttaata aagtatatca aagatagaga
    26761 gaatgacaat gttaaaaatt gaaaatgtta caggggggta tgttaatatc ccagttttaa
    26821 aaaatatttc ttttgaagta aatgatggag agttagttgg attaattggg cttaatggtg
    26881 caggaaaatc aaccactatt aacgagatta ttggtatttt gagaccctat caaggagata
    26941 ttaccattga tggcattagt cttgaagcca accaagaact ttatagaaag aaaataggct
    27001 ttattcctga gacaccaagt ctctatgagg aactaactct cagagaacat ttagaaacgg
    27061 tagctatggc gtatgatatt gccaccgatg aagttatggc acgtgcgcaa aaactattag
    27121 agatgtttcg tctaacagat aaattagact ggtttccaat gcatttttca aagggaatga
    27181 aacaaaaggt tatgattatc tgtgcttttg ttgtatctcc aagtttattt attgttgatg
    27241 aaccattttt aggtttggat ccactagcta tttcagattt gattaatctt ctagcagaag
    27301 aaaaagctaa agggaaatct attttgatgt caacacacgt attagattct gccgaaaaaa
    27361 tgtgtgatcg ttttgttatt ttacataaag gagaaatacg ggcagttgga accctagagg
    27421 aattacgggc tatttttgga gacagtaatg ctaatttaaa tgacatctac atagctctga
    27481 caaaagaggt gtgatatgaa aaagttattt aataaacgtc gaagtctctt tttaactcaa
    27541 aatagtaaat atttgagata cgtctttaat gatcattttg tattagtatt aatgtttttg
    27601 tcaggttttt tgttatatca gtatagccag ttactaaaag actttccaaa aacacattgg
    27661 ccaattatcg tgattgtgtc aatcattatc ttaatgttac tagcgatggg ggaaatagcc
    27721 agctatttag aaccggctga taaacaattt ttacttataa aagaagaagc aataaaggaa
    27781 attataaatt ctgctaaaaa acggacttat atcttttggt tggttgttca aaccttattt
    27841 ttagtattaa tatcaccaat tttgataaag ttaggcttgt cagtttttat gattaca
//
LOCUS       DATRSG010000021        26931 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRSG010000021 DATRSG010000000
VERSION     DATRSG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26931
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.021"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(34..1173)
                     /gene="recA"
                     /locus_tag="VBD89_000886"
     CDS             complement(34..1173)
                     /gene="recA"
                     /locus_tag="VBD89_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO7135430.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(1247..2506)
                     /locus_tag="VBD89_000887"
     CDS             complement(1247..2506)
                     /locus_tag="VBD89_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO7135431.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQMVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKIGEVTL
                     RLSTKAENQNEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTSGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(2595..3146)
                     /locus_tag="VBD89_000888"
     CDS             complement(2595..3146)
                     /locus_tag="VBD89_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO7135432.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRSIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(3169..3759)
                     /gene="ruvA"
                     /locus_tag="VBD89_000889"
     CDS             complement(3169..3759)
                     /gene="ruvA"
                     /locus_tag="VBD89_000889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO7135433.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNDTVEQYIKSSLKMLMK"
     gene            complement(3761..4993)
                     /locus_tag="VBD89_000890"
     CDS             complement(3761..4993)
                     /locus_tag="VBD89_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7135434.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYSPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCLVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSVLGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(5025..6998)
                     /gene="mutL"
                     /locus_tag="VBD89_000891"
     CDS             complement(5025..6998)
                     /gene="mutL"
                     /locus_tag="VBD89_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO7135435.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQSGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            7224..7427
                     /locus_tag="VBD89_000892"
     CDS             7224..7427
                     /locus_tag="VBD89_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO7135436.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(7484..10060)
                     /gene="mutS"
                     /locus_tag="VBD89_000893"
     CDS             complement(7484..10060)
                     /gene="mutS"
                     /locus_tag="VBD89_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO7135437.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIEPGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDPGKQQGQLSLFEEHSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(10117..10554)
                     /gene="argR"
                     /locus_tag="VBD89_000894"
     CDS             complement(10117..10554)
                     /gene="argR"
                     /locus_tag="VBD89_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7135438.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            10776..12467
                     /locus_tag="VBD89_000895"
     CDS             10776..12467
                     /locus_tag="VBD89_000895"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO7135439.1"
                     /translation="MDTKHLIASEIQKVVPYMEQSAILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGSYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAEIDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            12555..12863
                     /locus_tag="VBD89_000896"
     CDS             12555..12863
                     /locus_tag="VBD89_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7135440.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(12890..13762)
                     /locus_tag="VBD89_000897"
     CDS             complement(12890..13762)
                     /locus_tag="VBD89_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7135441.1"
                     /translation="MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(13870..14814)
                     /locus_tag="VBD89_000898"
     CDS             complement(13870..14814)
                     /locus_tag="VBD89_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7135442.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            complement(14804..16555)
                     /gene="aspS"
                     /locus_tag="VBD89_000899"
     CDS             complement(14804..16555)
                     /gene="aspS"
                     /locus_tag="VBD89_000899"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO7135443.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVAASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(16648..17928)
                     /locus_tag="VBD89_000900"
     CDS             complement(16648..17928)
                     /locus_tag="VBD89_000900"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO7135444.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPTTDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            18148..18330
                     /gene="rpmF"
                     /locus_tag="VBD89_000901"
     CDS             18148..18330
                     /gene="rpmF"
                     /locus_tag="VBD89_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO7135445.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            18346..18495
                     /gene="rpmG"
                     /locus_tag="VBD89_000902"
     CDS             18346..18495
                     /gene="rpmG"
                     /locus_tag="VBD89_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7135446.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            18775..19401
                     /locus_tag="VBD89_000903"
     CDS             18775..19401
                     /locus_tag="VBD89_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO7135447.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            19413..19751
                     /gene="cadX"
                     /locus_tag="VBD89_000904"
     CDS             19413..19751
                     /gene="cadX"
                     /locus_tag="VBD89_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO7135448.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            19802..21217
                     /locus_tag="VBD89_000905"
     CDS             19802..21217
                     /locus_tag="VBD89_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135449.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKYNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDEKLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSYNPARS
                     FRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILFELGGNDIPSTFRQENI
                     DPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILERMYKENIPKSYLTSVPL
                     VISQKGRTTYSFSMTGGQQIDGVKFTQIYEDYMKLLSQGKDIAELYQKYSKEELANLG
                     INIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLEPTRFSLEDKKRIEEAA
                     AQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYYFQEQLNKELLSMAHAL
                     DNVQQAFIKLLSEEEIRKFAL"
     gene            21538..21987
                     /locus_tag="VBD89_000906"
     CDS             21538..21987
                     /locus_tag="VBD89_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7135450.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            22189..22509
                     /locus_tag="VBD89_000907"
     CDS             22189..22509
                     /locus_tag="VBD89_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135451.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            22509..23609
                     /locus_tag="VBD89_000908"
     CDS             22509..23609
                     /locus_tag="VBD89_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7135452.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            23606..24211
                     /locus_tag="VBD89_000909"
     CDS             23606..24211
                     /locus_tag="VBD89_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135453.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            24417..25022
                     /locus_tag="VBD89_000910"
     CDS             24417..25022
                     /locus_tag="VBD89_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO7135454.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            25015..25263
                     /locus_tag="VBD89_000911"
     CDS             25015..25263
                     /locus_tag="VBD89_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7135455.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            25353..26618
                     /locus_tag="VBD89_000912"
     CDS             25353..26618
                     /locus_tag="VBD89_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7135456.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
BASE COUNT         8802 a         5277 c         4337 g         8515 t
ORIGIN      
        1 atttattcaa gttcaagatg tatagttgct attttattcc tcaatttcaa tagcattgtc
       61 caaatctaaa acaagatcat cagcttcatt tttttcttca acgagctcag actgaactgg
      121 cgaatcatct tcagtcattc caaagtgcac gcgcacttta tggtcaattt catcaaaaat
      181 tgctggatta tctgctaaat attttttggc attttcagaa ccttgtccaa ttttctcacc
      241 attatatgag taccatgcac ctgctttttg gataatatcc aaatcactag caattttaat
      301 taactcaccc gtgcgagaaa taccttcacc atacataatt tcgacaaaag cttcacggaa
      361 tggtggagca actttatttt taacaacttt gatttttgtc tctttaccaa cattatgatc
      421 tttatgctca ccagtcccct tgatttgagt attaccacga acatctagac gaaccgatga
      481 ataaaatttc aaagcacgac caccaggtgt tgtttcaggg ttaccaaaca taacgcctac
      541 tttttccctt agctggttga taaaaattgc gattgtcttt gtcttgttaa tggatgctga
      601 caatttacgc attgcttgac tcatcatacg agcttgcaaa ccaacgtggc tgtcaccaat
      661 atcaccatca atctcagcac gaggaacaag ggctgcaact gaatcgacaa caactaaatc
      721 aactgcgcct gaatcaatca atttacctgc aatttcaaga ccttgctcac ctgagtcagg
      781 ttgagacaag agtaactcat caatattaac tccaagagcc gcagcatagg ctgggtcaag
      841 agcgtgctca gcatcaataa aggcagcaat cccaccttcc ttttgggctt gagctacggc
      901 atgaagggca accgttgttt taccagatga ctctggtcca taaatttcga cgatacgtcc
      961 tttaggatat ccacctgctc ctaaagcgat atctaaagca agagaacctg aactcattac
     1021 ttgaaccttt tgttcagctc gctcacctaa gcgcatcact gcgcccttac caaaatcttt
     1081 ttcaatattt ttcaaagcat catctaaggc ttttcgacgt tcatcaccga atttcttagt
     1141 aatttcttct gcttttttcg tttttttagc caatgtcttc tccttatatt tctatgaatc
     1201 taggttgatt caaatttaga aatcgttcta ctaattataa caaatattat atctttaata
     1261 aagccctacg caccaggtca aaagcatgta aagtactgat atgacggaca tctgagcggc
     1321 tcttccctcc aatcgatact ttaatagaag aaacgccttc aggggttgca atcccaataa
     1381 aaactgtacc tgcagggtat ccttccaact catcaggacc agcaactccc gttaaaccga
     1441 taccaaaatc tgcttttaaa agctggcgag cttgctgagc cattgcctca gctgtaaaat
     1501 gactgacaac tccatattct tgcaattttt tcttagggat gccgagaagt tgtgattttg
     1561 cttccatact ataggtcgta aagccaccat taaaaacttg cgaagcacca gaaaattctg
     1621 ctaactgaga ttgaaataac cctgatgtta agctctctgc ggcagtaatc gttttatttt
     1681 gtactttcaa taattctaaa acagtacgtg ccatactatt attatcacca tagccatata
     1741 ataaatcttt taatttacgg ttatctaatg tcttaagagc aagtatttct ttttctaagc
     1801 tatctaattt aaagtcggct tcattctgat tttcagcttt cgttgataac cttaaagtta
     1861 cctcaccaat ctttgcatat ggtgctatag ttggatctgt ctgattctta attatgtctt
     1921 ctaagattgt gactaactga ctctcaccga taccaaagaa tctcaataca cgtgaatata
     1981 attttgtgcc attatttgac aaaataggta ccacctgctc cataaccatt ggctttaatt
     2041 cactaggcgg acctggtaaa accatatatt ggactccctt agatgtaacg ataccaccaa
     2101 cagctagacc agtaaggttt tgtagaggta ttgagccttc aaccaattga gcttgtcgtt
     2161 cattatttgg cgttctagca aactgaggac gacttgcaaa aaaacgatcc aatttttgac
     2221 tagctaataa atccactgtt aatgatttac ctaaaaattt agccaaagtt tgtttcgtta
     2281 aatcatcctc tgtaggtcca agtccaccac ataggataac catttgactg cgctgactag
     2341 ctatatctaa aagcgataat aaacgttcct cattatctcc tacagctgtc tggaaataaa
     2401 tatcaattcc caattctgcc attttctccg acaaaaattg tgcgtttgta ttaacgatct
     2461 gtcctgttaa aatttctgtt cctacagcga taatttcagc cctcacaact tcaccctctt
     2521 atctattcga aataatgact acattttagc aaaaaaagta gcagaagtgc tacttaaaat
     2581 atatcacatt gatattactt gaaatcacat atattttcat ggtcattaac catacccgcg
     2641 gcctgaataa aagaatagag gcaagttgga ccaacaaatt taaaaccgcg tttttttaaa
     2701 tccttagata agcgttcgga taaggtagta cttgcaggca catcgttgta gttgttaact
     2761 gagttaacct gtactttatt atctacaaaa gaccaaatgg agcgatcaaa acttccgaat
     2821 tctttctgaa ttaatataat tgacctagca ttttgtcgtg ttgagaaaag ttttagacga
     2881 tgacgaataa ccctaggatt ttgtaagatt atctctaaat ccgcatctga cattgcagct
     2941 accttttcaa tattatagtg atgaaaaact tgtctgaact cctggcgttt atttaatact
     3001 gtttcccaag ataaacccga ttgataagtt tctaaacata gtaactcaaa gagtacatga
     3061 tcgtcatgaa cagctctccc ccactcttta tcatggtaag ctacatataa tggattatct
     3121 aaattcaccc aagaacatcg tttcatgtga aacctcatta cttctaaatt atttcatcaa
     3181 catcttcaaa ctagatttga tgtattgctc cacagtatcg ttcgtacctt caaaaaaggc
     3241 ttttactttt ttcaattcag tcgccttata ccccaacgct aacaaagctt ccatagcttc
     3301 ttcaagattg ctattctgct cagagctaac cttacgacta gtcgcactct caccagaagc
     3361 ttctacaaac ttccctgaca aatcaaggat catctgttgt gctgtttttt ttcctatctt
     3421 tggaaattta gtcaaatact taatatcact attatcaatg gcacttacga gaccttcatt
     3481 gtcatcgact gcaataattg ctaaagcggt cgttggacca attcctgata ctgaaattaa
     3541 attaagaaaa atttcttttt cattttccgt atgaaaacca aataaaagat gtgcatcatc
     3601 gcgaatgact tgatgtaagt agatggtaac ttcttgatta acgtaacctg aaaaagagta
     3661 gggatttgcc acatatatca cgtatcccaa tccagctgtt tccacaacaa taaatttagc
     3721 cgttatttta ctcaattttc ctttaatata atcgtacatt ctattttcct atctcaatat
     3781 ttttcatttt ggtagcgtat aagattaaaa ccagacctgc taacataaaa atggtcaatg
     3841 ttatttgaac acctattttt cctgtaaaat agctgagcga aaccatggca cctgctaata
     3901 cacttgcaac aggttgtgcg atagctaaaa atccagaata tgatccaatt tttccctcca
     3961 ccatcatctc cgcccttaga acctgactag ctggaacata aatcatttcg ccaaaggtga
     4021 aaaacgtcat ggcaatcaaa atagctccaa aagtgtttag agaaatatta aaaagcattc
     4081 caaaaccaca aatcaagctt cctaatatta gttgacgttt caaaggccaa ttctctatga
     4141 atttatttat agttgtcatt agtaagacaa ttaatagagt gttcgtaaag actgctaaac
     4201 taagcatttt agcacctgtg attgtatgtc ctaacacaga cactacttca aaattttgct
     4261 taagattgac cgagaaataa ttatcaacct ggagccagac aacactggaa ccaattgctc
     4321 ctaaagtata aacaacaaac gctttatcca ctaaaacctg actataattt ttaaaagttc
     4381 ctaaaactcc cttatcatgc ttgaaggttc cctcctgtgg ttttgtctca tcaaacttga
     4441 agtagaccac caaacaacaa atcgctgaaa taataagaag aactattaat aattcaaaaa
     4501 aatggtgatc atagaaaatc ccagcaatcc cagaacctag cattacagcg atgttaacca
     4561 accaataacc tatagtgtaa acaaagcgac gattctgttc gttcgtcaaa tcaatggtca
     4621 tagcttcata ggcaggggaa taaaattggt gagctatttc aatgataagg atacctacaa
     4681 aggtcaagtg tggcgtaata tgatttggaa catttgcagc tatagttata gcccaaccaa
     4741 tggttgccag cagtgaacct acaatcacaa cttttttcct ccctatagca tctgacaaat
     4801 ggcctccata taaggtcgct acaaaccctg atagttgagt tatgatgatc aaaattcctg
     4861 taactagatt tccaaaatat tgaacataat acattgccat aaaaggaaaa atagctgaac
     4921 caaccgtaat ggaaataaaa cgcaataatt ctcttaactg tagctgtttt ggcaaagcaa
     4981 aaaactcttt cactttttct cccttcttcc ctaacaagta attatcaata ttttcctaaa
     5041 tccctgaggc ttgtatgatt ttcttggatt cgtttaaaca ttttctccat atcagctttt
     5101 gtaaaattaa ctaaaacagg tcttccatga gggcaattat aaggattttt acattgcgct
     5161 aactgatcta ataaatgacg tgctgagtaa tcgtctagcg tatggtttgc tttaatagat
     5221 cgcttgcaag acatcataat agccaactct gcgcgatatt ttttcactga tacttcatta
     5281 gtgagcaaca acatatcaca catttcataa acgccagact ctacctcttc ttctttcatc
     5341 caaattggat gttcccttaa aatgaaagta ttattaccat acggttccaa gaaaatacca
     5401 acatcttgta ataatgacat tttttcttgt aattgaagaa aatctgaact tgaaaattca
     5461 aatagaaatg gtactaacaa ttgttgaaga ctattatcaa cctcacctat cttttcacgg
     5521 taatactcgt attttacacg ctcttgagct gcatgttgat caataatata caacccacca
     5581 ttcccttgag caaataagta ggtcccatgc atttgaccaa aaaattcgag ctctggaaaa
     5641 gtcgaacttt cttccgaatc taatttttca acaagtttgt taagtgactg tttactacta
     5701 aggtagtcct gagaatgaat gaagttctca ctatcagctt gatctctact agcatacttt
     5761 atattaggag actgcgttgc tccttgatta acagagtttt ttacatcatt gtcaactgaa
     5821 ccgtcacttt tgtcaaggtt tgtaaagtta gcaataggtt cagatacagt atctgcatct
     5881 ataaaaaagt catttttagt cctatcgtaa tacaaaccag actgttttag tgagaagctt
     5941 gtttgtatag gcttatctac acttcgggta ctagttttcg ctaaattctc taaagcatcc
     6001 ggaatcaaat catattgttt cagactctca gaaattgctg agcttattaa actcattaat
     6061 tctcgttcct tagaaattct cacctcttgc ttagtcggat gaacgttgac atctgctaga
     6121 taaggatcta tctgaatatc aatgacagca attggaaatc ttccaaccat caactttgaa
     6181 ccataaccat ctagaattga gcggtttaaa agaaaatttt taatgtaacg accgttaatt
     6241 aatagggtaa tatagtttcg gttagctcgt gtcaattcag gcaaacttac ataacccgaa
     6301 atttcaaaat ctaaatctgc atttgaaatc tcaatcattt tttttgcagt atttaaacca
     6361 taaatacctg caatcgcctg tctcaaatcg ccagtcccac tagttttagt catttcttta
     6421 ccatcattta tcagtgtaaa agcaacttcc ggatgcgcta gactgagtct attaacaata
     6481 tcaataatat gagctagttc agactgtaaa ctcttcatgt atttcaatct ggcaggcgta
     6541 ttaaagaata aattttcaac taaaattttt gttccacgag gacttgaaac aacttcctgt
     6601 ttttcaatgt ttcccccttt agcaacaagt aacgtccctt gtttcccttg ctctgtagct
     6661 gtttttattg tcattaaact aatcgaagcg attgatggta gagcttcacc acgaaaacct
     6721 aaggtccgaa tacgaaaaag gtcactctgg cttttgattt tggatgtagc gtgacgtcgt
     6781 aaactgagaa cggcatcctc actagtcatc ccttcaccgt tatccgttat ttgaatcttt
     6841 ttaagaccag attcttcaac ctctatggta atttggctac ttcctgcatc tatggcattt
     6901 tcaactaatt ctttaacgac gctactaggc ctttcaacaa cctcaccagc agcaatctgg
     6961 ttagctaata tatccggtaa ttcaataatt ttagacaagt tcatccctcc tttttttatg
     7021 attttttgta actttttttg tgatatctta gccattatac catattcaaa atattaattt
     7081 gacaataaca caatttttag ttgacatcac tcctattacc tgttagactt agggagcgta
     7141 ataagttttg aagttgtatt tttgtttaac atttttacct tttttatttt ttacagtaac
     7201 atttttagaa aggtaataaa caaatgacac aaggtacagt gaaatggttt aactcagaaa
     7261 aaggttttgg ctttatttct agcgaaactg gaacagacgt cttcgctcat ttttcagaga
     7321 ttaaagttga tggcttcaaa actcttgaag aaggacaaaa agtaacattt gatattcaag
     7381 atggtcaacg tggaccacaa gcaacaaata tcaatcttgt taaataatta aaaaagagcc
     7441 taaatgaatt atttagtctc ttttttgttg cctaaaggct attttataat aatttcttta
     7501 aatcaaatat agcattcata gcttgcattg gagttagatt cattatatct atcgcttcta
     7561 actcctcaat aaccctacct gcatttgaat gttcttcaaa aagcgataat tgtccttgtt
     7621 gcttcccagg atctagttcc tcatttaagt cagcagtaac agcttcttgg ggggctgata
     7681 cgatcaactg tactgcatca gcttccaact gtgataaaat atcagttgcc ctatccaata
     7741 agtcaattgg taaaccagct atttttgcga cgtgtatccc ataagacttg tccgcaggtc
     7801 caggttcaat tttatgtaag aaggtaactt ctccatctct ctctaaagta gccacgtgta
     7861 cattgacaag ccttgtcaat tgttcagata aatctgtcaa ctcatggtaa tgagttgcaa
     7921 acattgtttt tgccctaaca cggtcatgaa tatattctat aatcgattga gctaatgcca
     7981 taccatcata agtggctgtc cctcgaccta attcatcaaa aagaatcaaa gatttatcac
     8041 tggctcgttt tacagcttga ttcgcttcca tcatttctac cataaaggtt gattgcccag
     8101 aaattaagtc atcagcagca ccaatcctag taaatattgc atcaaataca ggcaaatcaa
     8161 cttcgtctgc cgatacaaaa cctcccattt gtgccataat aactgtcaga gctaactggc
     8221 gcatataggt cgacttacca ctcatatttg gtcctgtaat caactggata tctgtctgac
     8281 tatcaaaata gatactattg ggaatatatt cttgcactcc catcactttt tcaacggttg
     8341 catgacgccc attcttaatc ttaatctgat gttcatcatt aaatttggga cgaacatagt
     8401 gatgattttc tgcaactact gccaaactct gtagaacatc aacggttgca atagtctttg
     8461 ctaactcttg aagacgttta atataagatt ctacttgagc acgaacacgc ataaaaatat
     8521 catattctaa atttgaagat tgctcgcgag cttcgagcat ttcaccttca attttggcta
     8581 gttctgctgt tccatagcgt tcagaatttt ttaatgtcgc tttacggaaa aaatgctccg
     8641 gtactagtga taaattggaa ttggtcacat ggaagtaata gccgtctttt ttattataat
     8701 caattttaag ggtaccgata ccacttgctg ctctttcctt agcttcaata tcagcaatcc
     8761 aacctgtacc ttcacgcatc actgttcgat aattatccaa ttgcttatca aatccagact
     8821 tgataatgtt tccctcagtg atagtagcct gtgcttctgg ggctatcgcc gtattaatta
     8881 aactttctaa ttcaggcata gtgtcaattt tgttgacaat gatgtcaagc tcaggttgat
     8941 tgaaggactg taaaatcatt ttaatccgag gaatttgtga taaggtctgt ccgagttgca
     9001 atagatcttt agggttggct tttccgaaag atactcgact tgctaggcgt tcaatatcat
     9061 atacaccctt taaactttct gtgagatcgt ttctctcaaa aaaataatca agaaacactt
     9121 gaataatatc ttgacgttcc ttgattcgat tcatacttac caaaggcctg tcaatccaag
     9181 ttctcagcat tcgagttccc atcgctgttt tagtctcatc taacaaccag taaagacttc
     9241 cgtgcttctt gcttgttcta gcattctcca gtaaatctag actatttttc gttgcatatg
     9301 acatttgtaa atagtccttt atttcataat gaactacttt ctgtaaatga ctaagttcac
     9361 gcttttgtgt tctatgcaca tattgtaaaa gtttttccgc agcagatatt tccaaatcag
     9421 ttaactgctc atcaatcaaa tgaatatcat taagtcgttc gtcttcgtat gataaaagca
     9481 agttcatctg cttcgtcagt aggtgatttt tttcgtccgt caattgatat cctactacaa
     9541 tctcacgagc ttttaaattt agtatttcac tacggacgga ctcaaaatct gttaaaaggg
     9601 ttgcctgaaa ctctccagtt gagacatcca tatatgctag accaaatgtt tgttgatctt
     9661 gcgaatcaat cgctactaag aaattattag cactatccgg cttcgttgac tccacaaccg
     9721 ttcctggggt aacaacttgc actacctcac gcttgaccac tcccacagct tttttaggat
     9781 cttccatctg ctcagcaatg gctactttgt aacctaattc aactaaaacg tcaatatact
     9841 gttgagctga gtgataggga actcctgcca ttgggattgg cttttctgcg ttcttatttc
     9901 gactagtcaa gctaatttcc aggatttgtg ctgcttttac cgcatcatca taaaataatt
     9961 cataaaaatc acccattcta aaaagcaaaa aagcatctgg ataattctct tttatatcca
    10021 gatactgttg cattcccggt gatatcgttg gcttggccat ttctcactcc taactaacgg
    10081 ggctggctgc catgctcagc tcctcaatca ttatatttat aaacctactg atagttcttt
    10141 ttcaaaaact ttggcaatat cttcattttg acaaatcacc aacaaggtgt ctgctcctgc
    10201 cattgttcct aaaatctctg gcagcccttt ctcatcaata aagtttgcta aaacgctagc
    10261 ctcccctaaa ttagtattta aaactaacat aaaacttgct cgcgatactt tcaaaatata
    10321 ggagcgtaag gctgggctaa atcgatttga agtagctgtt gataaactat aatatagctt
    10381 accttctggg ctacgcaatt ttaaaagacc aatctcacgc aagtctcttg atagagtcgc
    10441 ttgcgtgaca tcgattcctt cattttttaa atgtaattta atttcttctt gtgtgccaat
    10501 ctgccctgat tggattaaac gtttaatacg tttttggcgt tctattttat tcatgttatt
    10561 caatcctttt tagttagaat tatgcttacc aaattatatc aaaaaaaatt gtctttttca
    10621 cttagtagtg atatcgtaat cattctgtaa aaaagctata aagtaggcta gttctttaca
    10681 gaatgcatta tgaatttaat caacaagttc tcgtctattt ttcataatta tgttaaaata
    10741 gggtgactat attactaatt aaaataggag ataccatgga tacaaaacat ctaattgcga
    10801 gtgaaattca aaaagttgtt ccatatatgg aacaatcggc cattctttct ttattagaaa
    10861 ccccaaaaaa ttcgagcatg ggagatttag ctttcccagc attctctttg gctaaaactc
    10921 tacgcaaagc acctcaaata attgctagtg acattgctga acaaattaaa agcgaccaat
    10981 ttgaaaaggt ggaagctgtt ggatcttacg ttaacttttt ccttgataaa gccgcaatct
    11041 caagtcaagt tttaaaacaa gtcttatctg atggttctgc ctatgctact caaaatattg
    11101 gtgaaggacg aaatgttgcc attgatatgt ctagtccaaa tattgctaag ccattctcaa
    11161 ttggacacct tcgttcaaca gttattggtg atagtttagc taatattttt gataaaattg
    11221 gctatcatcc tgttaaaatt aatcaccttg gcgactgggg taaacaattt ggaatgttaa
    11281 tcgttgccta taaaaaatgg ggcaatgaag aggctgtccg tgctcatccc attgatgaac
    11341 ttttaaaact ttatgtccgt attaatgctg aagccgagat agaccctagc gttgatgaag
    11401 aggcacgtga gtggtttcgt aaacttgagg ctaacgatcc tgaagcaact gaattatggc
    11461 aatggttccg tgacgagtca ttattagaat tcaaccgtct ttacgataaa atgaatgtta
    11521 catttgatag ctataacggc gaggcattct acaacgacaa aatggatgaa gtgcttgaac
    11581 tgctagaatc taaaaacctc ctagtcgaat ctaaaggtgc acaagttgtt aacctagaaa
    11641 aatacggtat tgaacatcct gccctcatta aaaaatccga tggagcaact ctctacatta
    11701 cacgtgactt agctgctgca ctttaccgta aacgtaccta cgattttgca aaatcaatct
    11761 acgttgtagg aaacgaacag tctgcacact ttaaacaact aaaagctgtt cttaaagaaa
    11821 tggactacga ttggtccgat gatatgactc atgttccttt tggccttgta acaaaaggag
    11881 gagcaaaatt atcaactcgt aaaggaaatg taattctcct tgaacctact gttgcagaag
    11941 ctattaatcg tgcggcatct cagattgaag ctaaaaaccc taacctagca gataaagata
    12001 aagttgctca agcagttggt gtaggtgcta ttaaatttta cgatttgaaa actgatcgta
    12061 caaatggtta tgactttgac ttagaagcaa tggtatcatt tgaaggtgaa actgggccat
    12121 atgtacaata tgcacatgct cgtattcaat caatcttacg taaagctaac tttaacccat
    12181 ctaatagcga taattacagt ctcaacgatg ttgaaagctg ggaaatcatc aaactcatcc
    12241 aagattttcc tcgtattatt gtgcgtgcag cagataattt tgagccctct attattgcaa
    12301 aattcgctat caatttagct cagtgcttca ataaatatta cgcccacaca cgtattttag
    12361 atgaagatgc tgaaatcagt agtcgtctag cactttgcta tgcaactgct acagtattaa
    12421 aagaatctct tcgcctccta ggagttgatg ctccgaatga aatgtaagaa atgacattac
    12481 taagactgtc aaatttatga cagtcttaat tttttatatt ataataatag cacaataagt
    12541 tacaggagaa tgccatgcca tcagaaaaag aaatattaga tgctcttagt aaagtttata
    12601 gcgaagaagt aatacaagca gatgattatt ttcgtcaagc tattttcgag ctcgctagtc
    12661 aacttgaaaa agaggggatg aactcactcc tagctacaaa aattgactca cttatcaatc
    12721 aatacgtctt aacacatcaa tttgatgctc ctaaaagcat atttgatctt agtcgcttgg
    12781 taaaaacaaa agctagccat tacaagggaa ctgctatctc agctattatg cttggtagtt
    12841 tcttaagtgg aggaccaaaa taaaaataca ggccagacag cctgtatttt tatttatcgt
    12901 aatttcttcg tcgaatttta aaatctgagc taataacttc atctacattt ataacagaaa
    12961 taaaagcatc tgggtctaaa tcagccatga tttctttcac atcacgcact tcacttgggt
    13021 taagagctac gtacaagaca tcatgctctc tacctgagta ggcaccttgg ccccgtaaaa
    13081 aagttacacc cctattaatt tcatgcaaaa tggttgctgc tgcagattct gattctttgg
    13141 taataatgat cattccacga acagtgtagc ccccattttc aacatttgtt aacacttgac
    13201 taaatacaaa gctagctact aacgtatata gcatctgttg taaattaata taaacaagtg
    13261 aagacgttag aactatagca tcaataaaca ataaggtttg ccctaatttt attccagatt
    13321 tttcctctac aatacgacca ataatatctg caccacctgt agttgcacca tatctaaata
    13381 ctaaaccact accagttcct gctaaaattc cagcagctac tgcaactaac atcatatcgt
    13441 ttttcaaagg gactgttaca gggatttgtt gccaaaacca catgaaaaaa gacattaaca
    13501 ctgtaccata tatagttaat aataaggatt ttttccctaa aatcctagcc cctaatataa
    13561 ataacgggat attaaggaga agtgatgaaa gtgctggatt aactccgaaa agtgcatgga
    13621 taatcaaagt gacaccggaa atacctcctt cagccaaatg attagccata ttaaatttga
    13681 caaagccgaa agtatagaga gccacaccaa aggcaattaa aatagcttct tttatctttg
    13741 tttttaaatc cagtttcaac ataggcttaa tccttcctta ttcaaaaaag ctaaagttgt
    13801 ttaaacaact tagcttaata acatcataat gtttccttag tggcggttga ccaaaagtca
    13861 tatatacagt taatcatcat caacgaatcg tccaataata tgaacatttt ctgcaacgga
    13921 aacaaaggct tttggatctg cttttagcat taaatattta aaatcactaa attcttcacg
    13981 tgttaatata gttattaaaa ctgctttttt ctcatgatta taagtacctt cagcatcatt
    14041 gatacaggtt acaccacgat gtaaatcacg atggatacgt ttaatgacgc aatcaggctt
    14101 actggtaaca atcatcgctt gcatcttttt ttgctttgta aaaatggcat ctgtaactct
    14161 acttgataca aaaattgtca ccatcgagta caaagcgtac ttccaaccaa ataaaagacc
    14221 tgcaaataat aaaataatac cattaataat aaaagaaata ctacctacat ctctgcctgt
    14281 tttctttcta attgtcaaac tgataatatc tgttccccca ctagaaattc tagacttaaa
    14341 gctaaaacca acaccagctc ccataattaa gccaccaaag atagcattga ttaagggatc
    14401 agttgtaagg gtgatttgag gcattagttg aatgaagata gagcttacag ttactgtgat
    14461 aaacgtaaaa atagtaaatt tatgtccaat cttacgccaa gataatatca gcaaagggat
    14521 attaatggca taaaaagcta aagcaactgg tatttcaaaa gaaaaccagt gtttactgac
    14581 agctgaaata acctgagcta aaccagttgc tccacttgaa taaacatgtc caggttgaaa
    14641 aaagaaatta acagcaacac tggatagaat accatacagt aatgaagccg aaattttctc
    14701 tgcatatttt tctcgtgaga tactttttaa agtgtgtaag agaccaaatt ttttagccca
    14761 tacactgata atatatttta cctttttttc taaaggggtt cgtctagttt tcataacttt
    14821 ctacagtaag tgataattct tctaattgtt gttctgaaac gagactagga gcttgagtca
    14881 ttggatcact agccttattg tttttaggga atgcaataac ttctcgaata ttgtccttgc
    14941 ctgctaacaa cataacaaag cggtcaaggc ctattgccaa tccaccatgt ggtgggaagc
    15001 cgtaatccat agcttcaagt aagaaaccaa actgttcctg agctgattca gcgctaaatc
    15061 ctaaagcctt gaacatacgc tcttgtgtat ctttttggtt aatacgtagg ctaccaccgc
    15121 ctaactcata tccattcaaa acaatatcat aggcaactgc acgtacttta gccaaatccc
    15181 cttccaactc atgcgctgtc tccgctgttg gcaaggtgaa tggatgatga gccgacatat
    15241 atcgtccctc ttcttctgac cactcaaaca ttggccaatc aacaacccat aggaaattaa
    15301 atttagaata atcaatgagt tcaagttctt ttgcaatgcg tgtacgaagc gctcctaaag
    15361 tctcgttagc tacttcaaga ctatctgcta caaatagaat taagtcatta ttctctagct
    15421 gcaaagcttc tgtcaaccta ccttcaatag ctgtcaagaa cttagcaacc ggacctgaaa
    15481 tagtattatc tacatatttt agccatgcta aacctttggc accgtattgt tttgcaattt
    15541 ctgttaattt atcaatattt tttcgagagt acttgtccgc cttgtctttg acaacaatcg
    15601 ctttaactac tgaagcttct gagaacacct tgaagtcaac ttcttttaca atttctgtca
    15661 agtcttgtaa aagcatatca aaccttgtat caggcttatc agacccgtag ttattcattg
    15721 catcatcata tgccatcctt gggaatggta aagaaacctc taaaccttta gtgtctttca
    15781 ttactttagc aatcataccc tcaacaatgt cttgaatttc ttgatcacta aggaatgatg
    15841 tctctaagtc aacttgtgta aactcaggtt gacgatctcc acgtaaatcc tcatcacgaa
    15901 aacatttgac aatttgataa taacgatcaa atcctgcatt catcaatagc tgttttgtaa
    15961 tttgagggct ttgtggaagg gcataaaaat gtccttggtt gacacgtgat ggaaccaaat
    16021 agtctcgtgc cccctctgga gttgattttg ttagcatcgg agtttcgaca tcaataaact
    16081 ctaagttgtc aaggtaatta cggattgagt gtgttacttt cgcacgtaat ttgaaatttt
    16141 caagcatttc cggacgacga agatccaagt aacggtaacg catgcgtgta tcatcatttg
    16201 cctcaatacc atctttaatt tcaaatggtg ttgttttcga ggtattaaga accgataatt
    16261 cttgcacttt taattcaact tctcctgttg gtaaattatc attagcttgt tctcgtgctg
    16321 tcactacacc gctaacctct ataacaaatt cactacggag actttctgcg gttgccataa
    16381 ctgaggctgc tacttcttca ggattaataa ctaattgcat gattccctcg cgatcgcgaa
    16441 gatcaataaa aataaggcct ccaaggtcac gacgacgacc aacccatcct tttaaagtaa
    16501 tcgatgttcc tatgtgctca ctacgaacac gcccagcata catactacgt ttcatctatt
    16561 ttctccattt aaaatattca ttccaaccta ttataacaaa aagcacctaa aaaccccacc
    16621 cattaaggag agtttttagg aactgtttta caatcctaat tgtttaagca cagatgaaaa
    16681 atctgtttta atgttttcaa aactaacttt aacttcttga cgtgtttgat tattttttaa
    16741 accgacctct ttcgaatcta cttcactgct acctaatgtc ataatcactt tagcattaaa
    16801 agtatctgca gacttaaatt gtgctttgat tttacgaccc aaataatcac gctctacttt
    16861 aaaaccttga tgacgtattg attgtgccaa atctaaggca gctagattag cctctgatcc
    16921 taatacagct atatacagat ctatagtatt ttctattggt aaagaaatcc cttgcttatc
    16981 taatattaag agcaagcgtt ctaatcctaa tccaaaacca aatgctggtg tttctggtcc
    17041 accaaaatag gatactaagc cgtcataacg gcctccagca cagatcgtta attcattatc
    17101 ttcaatctca gtgataaact caaaaatagt atggttataa taatcaagac cacgaaccat
    17161 attagtatca atgatgtatg gaatatttag agcatcaagc atatgacaca cagcatcaaa
    17221 gtgggcttgg cttgactcat ctaaataatc taaaatagaa ggagcatttt caactgctag
    17281 tttgtcttct ggctccttag agtctagtac gcgcaatgga ttttcattca aacgacgttg
    17341 actatcctta gacaattgtt cacgtatggg tgttaagtaa tcaataagag cttggcgata
    17401 ggcttgacga ctttctggac ttcctaaact attaagatga agtttaacgt ttttgatacc
    17461 taaatcttca aatagatgat gtcccattgc gattgtttca acatcagttg ttggattatt
    17521 tgatccaaag cactcaacgc ctacttgatg aaattctcgt aaacgaccag cctgtgggcg
    17581 ctcatatcgg aacattgatc caatataata cattttggtt ggtttttgaa cttctggtgc
    17641 aaatagttta ttttcaacat aagaacgtac aacaggtgct gttccttctg gacgaagcgt
    17701 aatgtgacga tctcctttat catgaaaatc atacatctct tttgtaacga tatccgttgt
    17761 atccccaact gagcgactaa taacctcgta atgttcaaac attggtgtac gaatttcatc
    17821 ataatgatat tgtttaaaga gattgcgtat aacattttca acgtattgcc actttgctga
    17881 ttctccaggt aagatatcct gagtcccttt tggtttttga agtttcataa gtttacctct
    17941 tcgattagtt ttctctttaa acattctacc attttttctt atattttaaa caagcatata
    18001 tcatttgctt tagtttgata gtattttcac catgaaattg taaaaataaa aagttttgtc
    18061 aagtaacttt actttacaaa aaagatatgt tatcatagtg aagttgatga aaatcaaaac
    18121 ttacacatcg aaaggaagaa taagaaaatg gcagtacctg cacgtcacac ttcaaaagcg
    18181 aagaaaaaca aacgtcgtac acactacaaa ttgacagctc catctgtaca attcgacgaa
    18241 actactggag attactcacg ttcacaccgt gtatcactca aaggatacta caaaggacgt
    18301 aaaatcgcta aagctaacga agctaaataa tagaagggag ataccatgcg cgtaaatatt
    18361 acacttgaac ataaagaatc tggtgaacgc ttgtacctta cttcaaaaaa caaacgtaac
    18421 actccagacc gtcttcaatt gaaaaaatac tcaccaaaac ttcgtaaaca cgttgttttc
    18481 actgaggtta agtaagggtt tcagtacaca tcaatagacg tcaaaatatt gattttaagc
    18541 gatttttgat tttttgaaac tcattatatc gcactacaaa tcaataaaaa ctctaccttt
    18601 tactctacct tttttagata aatatatcat ctggatattg aagttaagcc ctgctatcat
    18661 ttcgatggca gggcttttta atatttgtac actaacttct tatctatatt ttggtggatt
    18721 ttaagttgac atctacaaag tttaatgtta gaatacattc aaaaatatat ttgaatgagg
    18781 tattttatga ttcaaaatgt tgttacttca ataatcctgt attctgggac agccgtagac
    18841 ttacttatta tcctaatgtt attttttgcc aaaagaaaaa gcagaaaaga catcattaac
    18901 atctatttag gacaatttct aggctctgtt agtctaatat tcctaagttt gctttttgca
    18961 tttgtcttaa attatattcc tagtaaagag attttaggtt tactcggttt gattccaatt
    19021 ttcctaggcc taaaagtttt gcttttagga gattctgatg gagaagctat tgcaaaagat
    19081 ggtttgcgaa aagacaataa aaacctgatt tttctagtcg ctatgattac ttttgcaagt
    19141 tgtggcgctg acaatattgg tgtctttgtc ccatatttta ccaccttaaa tttagcgaat
    19201 ttgatagtga ctttacttac ttttctagtc atgatttatc tcttggtttt ttctgcacaa
    19261 aaattagcac aagtcccttc tgttggagaa actttggaaa aatatagcag atggtttatt
    19321 gccgttgtct atttaggatt ggggatgtat atcctgattg aaaacaacag ctttgacatg
    19381 ctatgggctg tgttaggcta ggagaaaaaa ttatgaaaaa agatagtatc tgtcaagtgg
    19441 atgttataaa tcaacaaaat gttacaaccg caacgaacta ccttgaaaag gaaaaagtcc
    19501 aaaaatcact tcgcatttta tcaaaattta ccgataataa acagataaat atcatctttt
    19561 atctccttgc cgtcgaagaa ctctgtgtct gcgatatagc ctgtttatta aatctcagta
    19621 tggcatctgc ctcccaccat cttcgtaaac tagccaatca aaacatcttg gacactagaa
    19681 gagaggggaa aattatatat tattttataa aagatgagga aatcagagat ttttttaatc
    19741 aactaggata acaactattt ctactacttt ctacttttcc atgattatag ggggattata
    19801 tatgaaattt tttgaagaaa attattcaca agaaatacct actcgtatca aaaatttaag
    19861 aaaaaaatac aatattacac aaagcgaact aggcaatgca ggtcaagtta gtcaagttga
    19921 aagtggtaag cgaccaatta cgagttcgat gttagtttat ttaaatgctc taaccgcttc
    19981 gagctatacg tatatcgttt ttggagagtt agatgagttt attgagaatc tgtttcatta
    20041 ttttttcagt tctattttat atagagattt agaggctgta gatgaaaaac tatattcttt
    20101 catgagtgat gatttaattt caatccaatc aagttgtcta agtattgcca agacatttgc
    20161 aaatttcaat attcaaagaa aaagatttat gatttctact gaaactgaaa tggatacttt
    20221 tcacaagaaa gatgacattg acgtatgggt tggtggaaaa agttataacc ccgctagaag
    20281 ttttagaacc cgtaccatca atgaattgac tgtcattgat tttgaagaaa tgtttgatat
    20341 tctttggttg atgttagggg ataatttaat taaatcattt gaagttaatg tctgtggtat
    20401 cctatttgaa ttaggcggaa atgacatacc ctcaacattt aggcaagaaa acattgatcc
    20461 tctcatcaat aaatggtggt atgataatgt ttcgacagaa attatcccta atctaataaa
    20521 aaaactcaaa gaaaatccct tgtttaatat cggatttatg gtaaatgata tattagaaag
    20581 aatgtataaa gagaatattc caaaatctta ccttacatcc gtcccattag ttatttctca
    20641 aaaaggaaga acaacctatt cgtttagtat gactggtggt caacaaatag atggagtgaa
    20701 attcacacag atatatgagg actatatgaa attactcagt caaggtaagg atatcgcaga
    20761 gttatatcaa aaatattcta aagaagagtt ggcaaatcta ggcattaata tttatcaatc
    20821 caatgatata gaaaggactg aggaaagaac ttttgatgaa attatcagtt gggtttccaa
    20881 cccttatgca acaagaccaa ttcaagaaag gcacactatt caattagagc caacaagatt
    20941 ttcactagag gataagaaaa gaattgaaga agctgcagct caaggactaa gcgaaatcga
    21001 ccttattgat ttagttgacc tatatgatat taatttagac aatacaagcg tcaatcgcca
    21061 tattgtgggg ttattgacta ataacaccca agtaacatac tatttccaag aacaattaaa
    21121 taaggagttg ctgtcaatgg ctcacgcttt agataacgta caacaggcct ttattaaatt
    21181 attaagtgaa gaggagatac gaaaatttgc tctttaaaga ctaggtgaat cgcctagtct
    21241 ttttttgcgc ctcgttttac aaattagtct attgaagtaa aaaaagaagt aaaaaataaa
    21301 agaacattac gccttatact gaatatatca agaaaaactc ttgatataaa ttttaatagt
    21361 ccaaattgat ggccatcaaa ggacagattg aaacaaatat gatgacatga cgataaaggt
    21421 catgtcttgc atagaattgt ttcgctgttt ctttgttgtg ccttttggta tactcagcgt
    21481 tcaatatttg ctcattttgt ttccttctgt ccaaaggaca gaaaggagct catcattatg
    21541 agtaataaag tacaagaacg tcgtgagcgt aaaatcaaag aagctattaa agctaaaaat
    21601 tgggatgaag taactcgttt gcttcaacaa gaacaaagta acgcagaacg tcgtgaccga
    21661 taccacaata gacgaatcaa agacgaaaca atagctagca aaaatgccaa aaagtctgta
    21721 cgttatgatg tcattgcaag ttctgatttg aatcccgaag aagctttaat cctagaggag
    21781 ttaagacaag ctattcgtga agctaaagca tccctatcag aaattgacag taagattgtt
    21841 gaaatgatag cggaacaagg ttcaagctat aaagaaacgg ctcgctatat cactgaacat
    21901 tataagaaaa tgagtgatgt aactgttaaa tcccattatt gtaaagcgct aaaaaaatta
    21961 gctcctttat taaaagctta ccgctaatcc ctcaaatcca gtgtctctgt cactggattt
    22021 tttgtaaaaa aaaattaaaa attcaacaaa taactttgcg tttagctgtc tcattctcct
    22081 tagtaatgag gaggtggtca gataacctaa aaaacaaagt ccagctcatg acccaaaccg
    22141 agcctgtcgc attcacacta tctcttattc ataaggagga tttgccctat ggcgtttaaa
    22201 atgaaaacca caaaaggtgg ttatacaact acattggctg ataaaattat cagtcaaaaa
    22261 caaccgattt actcacttag taccgaactt gaaccacaac aacggtttga agaaggaaaa
    22321 ccaaccggag aaatcgtagc ttataaagca tggtttgtac aggaagggca agaccctttc
    22381 caagtaaaat ttgaagaaac gattgagctt ccagcttttc aatccatgat tcaatttgac
    22441 acactacaag cctgcgaagt aaaatataac atctacttca aagcaaatgg tatcaaggag
    22501 gttcggtaat gacttcacaa tctcgtgatg tgctgaacca atcttacctg caatcttacc
    22561 tgctccagag cctaaacatg gctctggggg ctttaatgca gggagaaacc agctacacca
    22621 attcttttaa tattgttatt caagcagacg gctttatctt tgtccctcgt ctaccatgtg
    22681 cttatatcct tgatgatgac ttgtacaaga aaatctttct gattgccaat gcttcacttt
    22741 atccacaata tacgcttcta aaacaaaacg ctacctattt tgtccctcta gaaacagatg
    22801 acttacacac tcagcgtgga ttatttttcc cttggaaaaa gggaatttca gaacgtttag
    22861 tcattccaga tttggataag ttttcagcta gcttgccaca ggggaaaatc cctatcatga
    22921 agtactttga actcaattta gataaggtta atcactgggc tattgctgga aattcaggtt
    22981 ctgggaaatc gtacgctctc acttatttct tgagtgtact aaagcatatg tctgacttga
    23041 ttatcattga ccctaaattt gataccccaa gtcgatgggc aagagaaaat cacgttgctg
    23101 ttatacaccc tgttgaaaac cgttcaaaat cagattttgt ctcacaggtc aatgaacaat
    23161 taagtcagtg tgcaactctc attcagaaac gacaagctat cttgtatgat aatcccaatc
    23221 atcaatttac ccatctaact attgttattg atgaagtcct cgctctatca gagggagtca
    23281 ataagaatat caaagaagcc tttttctccc tactctcaca gatttcctta ttaggacgtg
    23341 ctaccaaaat ccatctgttt ttggtaagcc agcgttttga ccacaatact attccaattt
    23401 cagtgaggga acagctaaac gtattgttgc aaattggaaa tatcaatcag aaaactactc
    23461 aatttttatt tccagaccta gacccagagg gtattgttat tccaatagga catgggacag
    23521 gtatcattca agttatcgat aatgaacatc cctaccaagt tctgccctta ctctgtccaa
    23581 cctactacac taaacgaggt attctatgac caataaaacc tactttatga gaagttttaa
    23641 tctcatacta tatttatttc tatttgcacc tatctgttct gcatttggat ggttattcaa
    23701 ttatctttcc gttcaattaa caaatatcac gtttgtcaat ttagatacgg tcaaaagtat
    23761 tacggatatc attagttccg tttgtcatgg atttgcactt atttctcttc tacttgcctt
    23821 atttttggtt ggattagaaa taatacaacg gttgaaaaca gatagtcttt ggaactatat
    23881 ccaatctgtc tatcacactt ttctgttgcg acacttcctg tttcagcgtg aacgagtaca
    23941 gaagataagc aatcttgaac atcagacggt tactactatc aacccaatcc ataacggatt
    24001 taaccgagct gttcgtaagt gtatcgtaga tatacgaaaa gataccatca ctatttttct
    24061 aaaagtccct agagaccaac aagggcaaaa aatcctaaaa gagatggaag cacaattaaa
    24121 agaagaaatc gtcagccaac acgcagacta ttatttctcc tctcctattc gagttcgtaa
    24181 ccaattgtgg ttcactggac aaaaacgtta atcccttggg gctgtggctt gcgttaagca
    24241 aaagccagac agcccctttt tatattcttt tctatcatga cctattgggg tgactacgac
    24301 ccccaatagg tcaataatca ataatcacaa aaaattcaat tttttcaaaa aaactttgca
    24361 tacgacctgt catttctcct tagtaataga acccaaaaga aatgaggtaa ctgcctatgg
    24421 caaaagaaca gcgctcaacc aaatggactt ttctcttcta cgaggaaagc gccccagaga
    24481 actacttgaa tatattagag gaactccata ttccgtttat tcttagtcct tggcacgata
    24541 aggacgttaa tagacagacg ggagagttca agaaatcgca caaacacggt gctttcttct
    24601 ttgattcact gaaaagctat tcacaagtat caaatatcat cagtgacaaa ctaaacggtc
    24661 cagcacatgt ggaggtagtt atgtctccca ctggtttatt tgactacttt acccatgcgg
    24721 aaaatccaga taagaccccc tacaacattg aggatattga agtcggttgc ggctttaatt
    24781 tagaaaaatt cttgatggag atgaactcct cggattttat acatgaagta gttgatatta
    24841 tcgaagagaa cgactttacc gaatttgaag aattggtctg gtatgcccga gcaaacaata
    24901 ccaatttatt agggcttatc attgaacgta catatttctt cgctaaatac ctagattcac
    24961 gaagatacaa tcctaatcga ctccataatt caaacacaga ggagaaagag aataatgaat
    25021 aacgaaaaat tagaacaaat tgaacaatta ttgcaaacac ttatcaaact cattcaaatc
    25081 aaagaacaga acataccact aaagataata caacagcgag aattgctaaa acagctaaat
    25141 atctccccaa acacactaaa aacttgggaa caaaaaggat taaaaagatt agaacctcct
    25201 atagagggaa ctcgaaccgt cttctatcta ctagatgata ttatcaactt tttacaatcc
    25261 taattctgaa aatttgtgct aaaatagttt tatctaaaag aaagagtggt aactatttta
    25321 gcctacttca atatccatga agaaaggcaa atatggaaac tgaaacaatt attcataatg
    25381 gcaaaaaagt cattaaaaaa atcaaaaaag ataagtccat ctcatacact ttgaagggga
    25441 catttctggg aaaagatatc aaaactggta agcaagtcac tactaccatt acagccaaga
    25501 cactaaaaca gttagataga gcaatcattc aagcacgact agaatttgaa aaaaatggct
    25561 caacacgaga aaaagttatc gtcattgata cactggaaga tttagctgaa gaatggttta
    25621 aatcatataa aacctgggtt aactcgcata atactctgaa cagagtaaga ggttatcttg
    25681 ataattatat tatccctaaa tttggagatt acaaaccaga taaaattgaa tctgcggata
    25741 tccagctttg gttaaacgat ttggcaaaga aatcaaaaga gtccattgaa tctggtataa
    25801 agcgtgctga taaaggtagt gctaaagatt ttggtgctgt tatccataag ttgaaagata
    25861 tttttgacta tggaattaca aactatggac taatcactaa tcctgttagc actgttaaga
    25921 taccacctaa gccaaaatca aataagcaac gcatcatggt attacatgat gatggtcttg
    25981 taatatggct taattatctt gaaagtttag ataacaatag agccaatcgt agatttaagt
    26041 taatttgtaa tactctatta gcctccgctt tgcgaataaa tgaattactt gcattgacga
    26101 ttgatgacct agattttgaa aattcttcta ttaacgtcag taaaacattg atgtggaaaa
    26161 ccgcaaataa aaaagatggg acaaaagggg aagttatctg taaagctaca ccaaaaactg
    26221 atgcaggaaa tcgctctgtc cctgtccctc cccccataat agaaaaactc agagatttcc
    26281 accacgaaat gaacaagtat cttgaattgc acaacagaca tagaacaaaa ctgattttcc
    26341 ctacaatcta tgggaactat atgtgtgaca gaaacgagag aacaactctt aagaaaaggt
    26401 tagtaggtct tggattacca aactatggtt tccatttatt ccgacatact catgcttcaa
    26461 tgctacttaa tgctggaaca aattggaaag aactccaagt cagaatggga cataaatcca
    26521 tttcaactac aatggatact tacgctgaat tagcaccaca acgaaagtta gaagctgtgg
    26581 ggatttatct tgaaaagatt gctgagctaa cgcaataact actctaccat tcactctacc
    26641 ttttattatg tagcattcta gaaacattga tgtaccaacg tttctaacag ctaaaataga
    26701 aattatttaa ctcttactga agttaaataa ggattcatta cacgtcaata ggcagtaaaa
    26761 agattggttt tatgagtttt tgaaatttta tatttcacta cactgcaata taaatcagtt
    26821 gaatctctac atttttctct acattttggt aagaaaaaaa tgacggataa ctaaacccca
    26881 ctagcaagag ttagtggggt tttattatta tttataaatc aatttacttt a
//
LOCUS       DATRSG010000022        26603 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRSG010000022 DATRSG010000000
VERSION     DATRSG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26603
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.022"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            143..568
                     /gene="rplK"
                     /locus_tag="VBD89_000913"
     CDS             143..568
                     /gene="rplK"
                     /locus_tag="VBD89_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO7135457.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            672..1361
                     /gene="rplA"
                     /locus_tag="VBD89_000914"
     CDS             672..1361
                     /gene="rplA"
                     /locus_tag="VBD89_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO7135458.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
     gene            1750..3366
                     /gene="nikA"
                     /locus_tag="VBD89_000915"
     CDS             1750..3366
                     /gene="nikA"
                     /locus_tag="VBD89_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO7135459.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            3353..4297
                     /locus_tag="VBD89_000916"
     CDS             3353..4297
                     /locus_tag="VBD89_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135460.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            4297..5103
                     /locus_tag="VBD89_000917"
     CDS             4297..5103
                     /locus_tag="VBD89_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135461.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            5091..5879
                     /locus_tag="VBD89_000918"
     CDS             5091..5879
                     /locus_tag="VBD89_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135462.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            5866..6547
                     /locus_tag="VBD89_000919"
                     /pseudo
     CDS             5866..6547
                     /locus_tag="VBD89_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6668..7396
                     /locus_tag="VBD89_000920"
     CDS             6668..7396
                     /locus_tag="VBD89_000920"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO7135463.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            7412..7969
                     /gene="frr"
                     /locus_tag="VBD89_000921"
     CDS             7412..7969
                     /gene="frr"
                     /locus_tag="VBD89_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO7135464.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            8087..8941
                     /gene="cvfB"
                     /locus_tag="VBD89_000922"
     CDS             8087..8941
                     /gene="cvfB"
                     /locus_tag="VBD89_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO7135465.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            9079..9588
                     /gene="msrA"
                     /locus_tag="VBD89_000923"
     CDS             9079..9588
                     /gene="msrA"
                     /locus_tag="VBD89_000923"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO7135466.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            9585..9800
                     /locus_tag="VBD89_000924"
     CDS             9585..9800
                     /locus_tag="VBD89_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO7135467.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            9957..11729
                     /locus_tag="VBD89_000925"
     CDS             9957..11729
                     /locus_tag="VBD89_000925"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO7135468.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            11853..12839
                     /locus_tag="VBD89_000926"
     CDS             11853..12839
                     /locus_tag="VBD89_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO7135469.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(12930..13733)
                     /locus_tag="VBD89_000927"
     CDS             complement(12930..13733)
                     /locus_tag="VBD89_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO7135470.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            13925..14401
                     /locus_tag="VBD89_000928"
     CDS             13925..14401
                     /locus_tag="VBD89_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7135471.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            14723..15529
                     /locus_tag="VBD89_000929"
     CDS             14723..15529
                     /locus_tag="VBD89_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7135472.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DLDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            15924..16421
                     /gene="ybeY"
                     /locus_tag="VBD89_000930"
     CDS             15924..16421
                     /gene="ybeY"
                     /locus_tag="VBD89_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO7135473.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            16402..16800
                     /locus_tag="VBD89_000931"
     CDS             16402..16800
                     /locus_tag="VBD89_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO7135474.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            16842..17741
                     /gene="era"
                     /locus_tag="VBD89_000932"
     CDS             16842..17741
                     /gene="era"
                     /locus_tag="VBD89_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO7135475.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            17978..18379
                     /locus_tag="VBD89_000933"
     CDS             17978..18379
                     /locus_tag="VBD89_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135476.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIPILVAFIIMKGCLTSISDILIYFYL
                     HLVVVYYTIGMILSLGRIISPEHSMFNKLRK"
     gene            18839..19090
                     /locus_tag="VBD89_000934"
     CDS             18839..19090
                     /locus_tag="VBD89_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135477.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
     gene            19109..19363
                     /locus_tag="VBD89_000935"
     CDS             19109..19363
                     /locus_tag="VBD89_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7135478.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(19700..19894)
                     /locus_tag="VBD89_000936"
     CDS             complement(19700..19894)
                     /locus_tag="VBD89_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135479.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            19893..20087
                     /locus_tag="VBD89_000937"
     CDS             19893..20087
                     /locus_tag="VBD89_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135480.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            complement(20138..20314)
                     /locus_tag="VBD89_000938"
     CDS             complement(20138..20314)
                     /locus_tag="VBD89_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135481.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(20350..21939)
                     /locus_tag="VBD89_000939"
     CDS             complement(20350..21939)
                     /locus_tag="VBD89_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO7135482.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            complement(22000..22068)
                     /gene="shp2"
                     /locus_tag="VBD89_000940"
     CDS             complement(22000..22068)
                     /gene="shp2"
                     /locus_tag="VBD89_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO7135483.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            22156..23004
                     /gene="rgg2"
                     /locus_tag="VBD89_000941"
     CDS             22156..23004
                     /gene="rgg2"
                     /locus_tag="VBD89_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEO7135484.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDHSYFEHSHYLIDKVRSLLQDE
                     VNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVL
                     GNNKEC"
     gene            23153..23974
                     /gene="mutM"
                     /locus_tag="VBD89_000942"
     CDS             23153..23974
                     /gene="mutM"
                     /locus_tag="VBD89_000942"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO7135485.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            23965..24558
                     /locus_tag="VBD89_000943"
     CDS             23965..24558
                     /locus_tag="VBD89_000943"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO7135486.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            24682..25383
                     /locus_tag="VBD89_000944"
     CDS             24682..25383
                     /locus_tag="VBD89_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135487.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            25608..>26603
                     /locus_tag="VBD89_000945"
     CDS             25608..>26603
                     /locus_tag="VBD89_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135488.1"
                     /translation="MKQLNILPQEGFTLTLTLMVITSLLIVINFYIKLIEPNDMQQTH
                     MIRLFRIEPSRYFQSAELLEASSELFFRSLSFGIFFHLNHFAFWHGLTFALFLAGSRL
                     GIKVLCLPLYGRGKDQENPESLLNILFYLGIGIGVFLSLFFLLLGKQFSPYPFFSWYT
                     GIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHETMKEKIAKIDNIELLGIEMKDRDINV
                     SELSPLLFENLSGISYLNAIFLKRMSRYLQRKILVRLAVLSVLFGIELLFLLFFKEKI
                     NIPMNEHSFSKFLFLSAVPGYLIYIGESYSKFCFYHLDRPLLRYNFYRERENILAT"
BASE COUNT         8742 a         3939 c         5131 g         8791 t
ORIGIN      
        1 ttaaaatata atgaaattaa tggcttaaaa ctattgcttt tatttatgat tatagtataa
       61 tggtaaggta tgttataaac atgcctgtgg gaggtaaaaa tttctaatta ccgccaaaac
      121 cacaacagga ggatttttaa aaatggctaa aaaagttgaa aaacttgtaa aacttcaaat
      181 tcctgctggt aaagcaactc cagctccacc agttggacca gcacttggtc aagcaggaat
      241 caacatcatg ggattcacta aagagtttaa cgctcgtaca gctgatcaag ctggtatgat
      301 tattccagtt gttatctcag tatatgaaga caaatcattt gatttcatca ctaaaacacc
      361 accagctgct gttcttttga aaaaagctgc aggtgttgaa aaaggatcag gtgaacctaa
      421 caaaactaaa gttgcaacaa ttactcgtgc gcaagtacaa gaaattgctg aaactaaaat
      481 gccagattta aacgctgcaa accttgagtc tgcaatgcgt atgatcgaag gtactgctcg
      541 ttctatggga ttcactgtta ctgactaatc agaaacaccc acataacccg caagacttca
      601 tcgtagtttg atgagtgacg tgggagattt taaatcaaat cgattgacca cattacaagg
      661 agaattttaa aatggctaaa aaaagtaaaa acttacgtgc tgctcttgag aaaattgaca
      721 gcactaaagc atacagcgta gaagaagctg tagctcttgc aaaagaaact aactttgcta
      781 aatttgatgc aactgttgaa gtttcttata accttaacat tgacgttaaa aaagctgacc
      841 aacaaatccg tggtgctatg gttttaccag caggtactgg taaaacttca cgtgttcttg
      901 tttttgctcg tggtgctaaa gctgaagaag ctaaagcagc aggtgcagac tttgtcggtg
      961 aagatgactt agttgctaaa atccaaggtg gatggcttga ctttgatgtt gttatcgcaa
     1021 caccagatat gatggcactt gttggacgtc ttggacgtgt ccttggacct cgtaacttga
     1081 tgccaaaccc taaaactggt acagtaacta tggacgttgc taaagcagtt gaagagtcta
     1141 aaggtggtaa aattacttac cgtgctgata aagcaggtaa cgtccaagcc cttattggta
     1201 aagtttcatt tgatgatgct aaattagttg ataacttcaa agcatttaat gatgttattg
     1261 ttaaagctaa accagctact gcaaaaggaa cttacatcac aaacctttca attacaacaa
     1321 cacaaggtgt tggtatcaaa gttgatccta actcacttta atcaataaaa aactaggaat
     1381 tccttcctag ttttatttat tctatatact taactagtta agtatatatt ttaataatca
     1441 aaggatatta tatgtgtttc ttttgaaaaa tagcttttta tctgtattaa ccttcttaaa
     1501 gactaatgaa tgtgcgatgt gttttaagta acagatgaga ataaggaaaa atgaaattgg
     1561 cgatatggta tgtgactatt atgattattc tttagcaata atctattaaa atgagttcgc
     1621 agggaataag taaattttgt atcgttgtag attttttatt ttaagattct tgacaagaca
     1681 ttactaaaat gatttaatta agtttgctta aaaaaataat taactaaact taaaagaaag
     1741 ttggtaaaaa tgagacgtaa catattattg agcattactt gtttattgat ggtgactctg
     1801 actgcatgcc attcacagga tagcaaaagt cataaactaa acagtgataa actcacgctt
     1861 gcttggggag aagactttgg tgatgttaat ccgcatcgct ataaccctga tcagtttgtt
     1921 atccaggata tggtttatga ggggttggta cgatatggtg acaatgggaa aattgaacca
     1981 gctttagcga aaagctggag cattagtcaa gatggaaaaa cctacacttt taaattgaga
     2041 aatgctaaat attctgatgg cagtaacttt aatgctgcga atgtcaaacg taattttgat
     2101 agtatttttt cgaagtctaa tagaggtaat cataattggt ttaatttaac gaatcaattg
     2161 gaaaattatc gtgccttgaa tcaaagtact tttgaaatta aactgaaaca agcttatagt
     2221 gctactttat atgatctctc tatgattcga ccaattcgct ttttatctga tagcgctttc
     2281 cctaaaggtg atgatacaac taagaagaat gtaaaaaaac caattggaac gggtcaatgg
     2341 gttgtgaaaa gtaaaaagca aaatgaatat attaccttca aacgtaatga gaattactgg
     2401 ggaaagaaac caaaattaaa agaagtaacc gtaaaagtta tacccgatgc tcagacgcgt
     2461 gctttagcat tcgaatcagg tgacgttgat ttgatctatg ggaatggcat tattggttta
     2521 gacacttttg cccaatatac gaaggacaag aaatatgtca cagctatttc tcaacctatg
     2581 tcaacaagac ttttgttgct aaatgctaag gaaagtattt tccaagataa aaaagttcgt
     2641 caggcaatga atcatgctat tgacaaggtt tctattgcta agaacacctt tagaggaaca
     2701 gaaaagccag cagacactat tttttcaaaa tcaacatctc actcagatgc taagttaaat
     2761 ccttatagct ataatgttga taaagcaaat cagctattgg atcaagctgg ttggaagatg
     2821 ggaaaagata aggttcgtga aaaagatggt aaagctctaa ccttacgtct tccttatatc
     2881 gccactaagg caactgataa agacctggta acttatttcc aaggagaatg gcgtaaaatc
     2941 ggtattaatg tttctcttat tgctatggaa gaagatgatt attgggctaa tgctaaaaag
     3001 ggtaattttg atatgatgtt aacctattct tggggagcac catgggaccc tcatgcttgg
     3061 atgtcggcat taactgccaa ggcagatcat ggacatccag aaaatatagc tttagaaaat
     3121 ctagcaacta aaactgaaat ggatagactt attaagtcag ctctagtaga tcctaaggaa
     3181 gaaaatgttg atagagacta taagaaggtt cttgaattat tgcatgatga ggctgtatat
     3241 atcccattaa cttatcaatc ggtgatttct gtttatagga aaggtgattt taaaaccatg
     3301 cgttttgcgc cagaagaaaa ttcattccca ttacgctata tagaaaaaaa caatgtatct
     3361 aagtagttcg atcatcaaaa aaatattgtc agcttttttg gcattgtttt tcatatcact
     3421 tctaacattt attcttatca aactatcaac agttaatcca gcagaaaatt accttcgttt
     3481 atcaaaaata agtgttagtc cagaagcgct aaaagaagca gagcactatt taggtttaga
     3541 taagccatta tggaagcagt actggctgtg gttccaaaaa gcattaacag gagattttgg
     3601 atattcttat gtgctcaggt tgccagtatt agatttggtt ttacaacggt ttttggctac
     3661 tttattttta ggcacgagtg ccttcttgct aattgttacc atttctaccc cgttaggtgt
     3721 gtgggctggc ttacatgaat ctgctcggag tgatcacttg attcgatttt tgagtttttc
     3781 tagtgtgtct atgccaaatt tttgggttgc ttacttgttg atgctgctat tttcggcaaa
     3841 attaaatctt ctgcctgttt caggaggtaa cgatttacaa agtttaattt tgccaagtat
     3901 taccttaagt ttttctaccg taggtcaata catagccctt attcgaaaag ctattagtca
     3961 agaaaataga agtttaaatg tggaaaatgc ccgattaaga ggagtaaaag aacgttatat
     4021 tgtcacacat cacctcctta gaaatgcctt acctgcgata atgacagcac ttagcttgac
     4081 ttgggtttac ttattaacag gatcaattat tgttgaagaa attttttcat ggaatggaat
     4141 aggacgttta tttgtgacca gtctaagaac ttcagatctt ccagtaatac aagcttgtat
     4201 gctaattttt ggaaccttat ttttagctaa taattttatg acacagtgtt ttatgaattg
     4261 ggttgatcct cggttacgaa agtcaaggga gaaataatat caaaaagaat ttatttttcg
     4321 agtagcttac tggtattgtt agtgattagt gccatttttg cccctattct atctagcttt
     4381 gatccacaat acgtagattt atcacaaaaa ttattggctc ccaataatgt tcatttgtta
     4441 gggactgacc aattgggtag agatgtatta tctcgcttgc tttatggtgc tagatattca
     4501 ctgtttttag ctattattat tagcttattg gaattaacga ttggtatgtt tgtgggtctt
     4561 attgttggtt ggtatcaagg aaagttagaa aacctctttt tatggatagc taatattatt
     4621 ttagcttttc cgagcttttt gctctctctt gcaaccgtgg ggatcttagg tcatggtcta
     4681 gggaatttaa tttttgcaat tgtctttgtg gaatgggttt actatgctaa attaatgacc
     4741 aatttagtta agagtgcaaa aaaagagcct tatgtgataa atgcgcaaat tatgggactt
     4801 tcagtctggc atattttaag aaaacatatt tttccttttg tgtatcagcc aatcctcgtt
     4861 atggtcctta tgaatatagg aaatattatt ttaatgattt ctggcttttc tttcttaggg
     4921 attggtgtgc aacctaatgt cacagaatgg ggaatgatgt tgcacgatgc tagaggatat
     4981 tttaggacag ctacttggat gatgttatct cctggaattg cgattttttt aacagtattt
     5041 tcctttaata ctttaggcga tgccatagat aaaaaagatt ggaaacgaca atggaacagt
     5101 tagaaataag aaaattgtca ttacagatag gagaggtccc tgtactaaga gattttagtt
     5161 gtaaaataga tatgggagaa tcgctaacta ttattggtga gagtggttca gggaaaaccc
     5221 tcttggcaaa attattggtt ggtcacattc cacaaggtat gacagttaga ggaaacatat
     5281 tttttaaggg agttgattta ggtaaactaa ctgtaaagca gtggcagaaa ttaaggggac
     5341 gagatattgc ctatttagtg caaaatccca tgtctatgtt taatcctttt caaaaaattg
     5401 aagcgcatat tttggaaaca atccttagtc atgagaaatg ttcaaagaga gtagccttat
     5461 ctaaagcgtt agaatggatg aaacgtttaa atttagatga tgcgatatcc ctgttaaaaa
     5521 aatacccttt tgagcttagt ggaggaatgc tacaaagaat tatgttagcc actatattat
     5581 ctcttgatcc tcaggtaatc atattagatg agccgacctc agcggttgat tgtcataatt
     5641 gttctactat atcagctatt ttgcaggaat tgcaaaataa tggaaaaacc ttgattactg
     5701 taacgcatga ttatcagtta gctagagacc tggggggaca attattagtt ataagtgagg
     5761 gagaagttgt tgaacaggga caaacccaag ctattttaag caatcctcaa cataactata
     5821 cgaaagcact gacagtgcaa atggagtatg aaggagatat tcttaatgtt ggtttgtaat
     5881 catgtcggaa aaacatttgg acgacaagag gttttaaagg actgtcattt tcatcttaaa
     5941 agaggagaaa taattggtat aatgggtaaa agtggcagtg gtaaaagtag ccttgcccga
     6001 ctcattatag gactagatag tcctacttgt ggttcgatat attttcaagg aaaaatttac
     6061 acacctaaag atggtaaggc gcagattatc cttgtttttc aagatgcgct tagttcggtc
     6121 aatccatatt ttagtattga ggaaattttg aatgaagctt tttatggaaa aaaaaacaac
     6181 ttttgaatta tgtcaaatat tagaagcggt gggtttagat ggaacttatc taaaatataa
     6241 agctagacaa cttagtggtg gtcagctaca gagagtttgt atcgctagag ctttactctt
     6301 aaagcctaaa attattattt ttgatgaatc cttaagtgga ttagacccag taactcaaat
     6361 taaaatgcta cgtttattac aaaaaataaa acgccgttat gaattaagtt ttataatgat
     6421 ttctcatgac cctaaaattt gtcaagctat ctgtaaccgg gtctttctga taaaaaatgg
     6481 ttatttagta gaagataatg aattcttgaa gagagcgtgt tctactaact gtttgaccaa
     6541 tctctgaatt attatgtttt tcttatttta ggagttgata aagaaatcaa cttactagtg
     6601 tcattgctct attaaaattt tgccttttat ggtaaaataa taaagacaaa ataaggagaa
     6661 catagtagtg gaacctaaat atcaacgtat attaattaag ttatctggtg aagcattggc
     6721 gggagataag ggtgtcggta ttgacattcc taccgttcaa tctattgcaa aagaaattgc
     6781 cgaagtacac aattcaggtg ttcaaatagc acttgtcatt ggtggaggta atctttggcg
     6841 tggagaacct gcagcagaag cagggatgga tcgtgttcaa gctgattata cagggatgtt
     6901 agggacagta atgaatgctc ttgtaatggc tgatagcctt caacaatatg gggtagatac
     6961 tagagttcaa acagctattc caatgcaaac tgttgctgag ccttacgtac gtggtcgtgc
     7021 tttacgtcat ctagagaaga atcgtattgt tgtctttgga gcgggtattg gttcgcctta
     7081 tttctcaact gatacaacag cagcgcttcg tgctgctgag attgaggcag aagcaatttt
     7141 aatggctaaa aatggagtag atggtgttta taatgctgat ccaaagaaag atgccaatgc
     7201 tgtcaaattt gatgaattaa ctcacgttga agtaataaaa cgagggttaa aaatcatgga
     7261 tgcaaccgca tcaactattt caatggataa tgatattgac cttgttgttt tcaatatgaa
     7321 tgagactggt aatattaagc gtgttgttct tggagaacaa atcggaacaa ctgtttcaaa
     7381 caaagcatct gaataggagt gtataaaaaa tatgactaag gaaatcgtta caaaagctca
     7441 agagcgtttt gagcaatcac accaaagttt atcaagagaa tttgcaggga ttcgtgcagg
     7501 acgtgctaat gctagcttgc ttgatcgtat tcaagttgag tattatggag cgccaacccc
     7561 acttaaccaa ttagcttcta ttactgtacc tgaagctcgt gttcttttaa tttcaccatt
     7621 tgataaatca tcaatcaaag atattgagcg tgctattaac gaatcagatt taggtattaa
     7681 tcctgcaaat gatggttcag ttattcgtct tgttattcca gctttgacag aagaaacacg
     7741 tcgtgattta gctaaagaag tgaaaaaagt cggagaaaat gctaagattg ctatccgtaa
     7801 tattcgccgt gatgctatgg atgaagctaa gaagcaagaa aagaataaag aaattacaga
     7861 agatgacttg aaatctcttg aaaaagatat tcaaaaggct actgatgatg ctgtaaaaca
     7921 tattgatgag atgactgcta ataaagaaaa agaattgttg gaagtttaat agtaacgttt
     7981 gctatcttta ttatgattgg caagtaataa aatgattatg gaggtaactg atagaatcaa
     8041 aaccagatgg atttggtttg tcggttacct ttttaaaagg agaaaaatga atacattatt
     8101 agcgacagtc ataacaggct tagtaactga tgaaaacaaa gatttttact ttatccaaaa
     8161 agatggcttt acctttgcat tatcaaagtc agaaggagag catcacattg gtgaaatggt
     8221 taaaggattc gcctatacag atatgcaaca aaaagcacgt cttacaacta aagaaacatt
     8281 tgcgactcga gatcattatg gttgggggac agttactgag gtacgtaaag acttgggagt
     8341 atttcttgat actggtcttc cagataagca agtggttgtt tctttggatg ttcttcctga
     8401 attgaaagaa ctttggccta aaaaaggtga tcgcctctac gtttgcttag atgttgataa
     8461 aaaagatcgt ctctgggctc tccctgcaga tcctgaagtg tttcaacgaa tggcaacccc
     8521 tgcatataac aatatgcaaa atcaaaattg gccagcaatc gtttatcgct taaaattgtc
     8581 aggaactttt gtttatctac cagaaaataa tatgctcggc tttattcacc ctagtgaacg
     8641 ttacagtgaa cctcgtttag ggcaagtgct ggatgcacga gttattggct tcagagaagt
     8701 tgatagaacc ttaaatttat cattaaagcc tcgttctttt gaaatgttag aaaatgacgc
     8761 acaaatgatt ttaacgtatt tagaaagtaa cggaggattc atgactttaa atgataaatc
     8821 ttctccagag gaaattaaag ctacttttgg aatttccaaa ggtcagttta aaaaagcact
     8881 tggaggactc atgaaagcta aaaagatcaa acaagatcag ctaggtacag aattattata
     8941 aacttttgaa cttttaaagt tttctcacga ggtcgtttta gttatagcta gaattaatgg
     9001 ctaattatag aaaaaagctg agaggaaatt ttggcttttt ttgttataat gtatctatta
     9061 aaaagaatgg agaacgatat ggaacgtgcg atttttgctg gaggttgttt ttggtgtatg
     9121 gtacaaccat ttgaagaatt agatggtatt gaatctgttt tatctggata cactggagga
     9181 catgttgaga atccaactta taaagaagtt tgtagtaaaa ctactggaca tacagaagct
     9241 gttgaaatta tttttaatcc tgaaaaaatc tcttatgcag atttagttga gttatattgg
     9301 gcacaaacag atccaacaga cgcttttggt cagtttgaag atcgtgggga taattatcgt
     9361 cctgtcatct tttatgagaa tgaagagcaa cgtcagattg ctcaaaaatc aaaagataag
     9421 ctacaagctt cgggtagatt tgatagacca attgtaacca gcattgagcc tgctgatact
     9481 ttttacccag cagaagacta tcatcaagca ttctatcgga ctaaccctgc tcgttacgcc
     9541 ttgagtagtg ccagaagaca tgcatttcta gaggagaact ggcattgaga aaatcatttt
     9601 acagttggtt gatgacacaa agaaatccaa aatcaaatga accagtagca atcctagcag
     9661 attacgcttt tgatgaaacc acttttccaa aacatagttc ggattttgaa acagttagtc
     9721 gctatttaga agatgaggcc agtttttcct ttaatttaac agattttgat gatatttggg
     9781 aagattatct caatcattaa caataatata gagattgtat tttcaatctc ttttttaggt
     9841 tatagaaatt ggacacattg tctaagactg tctgaaaagc aggtttttta atggcaatcg
     9901 ttttcacaaa ggagtagaat atagtcatct aaaatatagc cctagggagg ttgtttatga
     9961 gatatacaaa tggaaatttt gaagcctttg caagacctcg aaaacctgaa ggtgtggata
    10021 aaaaatccgc ttatattgtt ggttctggtt tagcaggatt agctgccgct gtctttttaa
    10081 tacgtgacgg tcaaatggat ggtcaacgta ttcatatttt tgaagaacta cctctttctg
    10141 gaggatcact tgacggtgtc aaacgacctg atatcggttt tgtaacgcgt ggtggtcgtg
    10201 aaatggaaaa tcacttcgaa tgtatgtggg atatgtaccg ttccatcccc tctctcgaag
    10261 ttccagatgc ttcttatcta gatgaatttt attggcttga caaggatgat cccaattcat
    10321 ctaactgtcg cctcattcat aaacagggga atcgcttaga atctgatggt gattttacac
    10381 tcggaacaca ttccaaagag ttagttaagc tagtcatgga gactgaagag tctttaggtg
    10441 ctaagacgat tgaagaagtt ttttcaaaag aattttttga aagtaatttt tggacttatt
    10501 gggctactat gtttgccttt gagaaatggc attcagcgat tgaaatgcgt cgatatgcta
    10561 tgcgctttat ccatcatatt ggtggtctgc ctgatttcac ttcattaaaa tttaataaat
    10621 ataatcaata tgattctatg gtgaaaccaa tcatcagtta tttagagtct cataatgtag
    10681 atgttcaatt tgatagcaag gtaactaata tctccgtaga ctttaagaac ggtcagaagc
    10741 ttgcaaaagc cattcatctc acagttggtg gtgaagcaaa aacaattgat cttactccaa
    10801 atgattttgt ttttgtaacg aatggttcta tcactgaaag tacgaactat ggtagtcatg
    10861 atactgtagc taaacctaat accgatttgg gaggttcttg gaatctttgg gaaaacttag
    10921 ctgctcaatc tgatgaattt ggacatccta aagtcttcta caaagatatt ccaaaagagt
    10981 catggtttgt ttctgctact gcaacaataa aagatccagc tattgaacct tatatagagc
    11041 gtttaacaca tcgtgacctc catgatggta aagtaaatac cggaggcatt gttacagtta
    11101 cagattctaa ttggatgatg agttttgcta tccatcgcca accgcatttc aaagagcaaa
    11161 aggaaaatga aacaattgtt tggatttatg gactctattc aaatgtggaa ggtaattata
    11221 ttaaaaaacc aattgaagaa tgtactggtc gagaaatcac agaagagtgg ttatatcact
    11281 taggtgttcc tgaaatgaag attcatgatt tatctgataa acaatatgta agtacagttc
    11341 ctgtttatat gccttacatt actagttatt tcatgcctcg agttaaaggt gaccgaccag
    11401 atgttatccc acaaggatct gttaacctag ccttcattgg taattttgca gaatctccat
    11461 ccagagatac agtatttaca acggaatatt ctattagaac tgctatggaa gctgtttata
    11521 ctttcttaaa tattgaacgt ggtgttccag aagtctttaa ttcagcattt gatattcgtg
    11581 tcttgttgca atctttatac tatctcaatg ataagaaatc tgttgaagat atggatttac
    11641 caatacctgc cttaatgcgt aaagtaggca tgaaaaaaat tagaggaact tatttagaag
    11701 aattacttcg agaagctcat cttttataat gtaaaataaa atcgagaaaa cttgtttttc
    11761 tcgatttttt agttttacaa cgactaaaat caccaaagaa aatgaaattt tgttataatg
    11821 tagtcactta ctgtacgaaa aggaagaata atttgcaaga atactctatc gaaattactt
    11881 tacaacatcc agatgatatg atgtcactct ttggcagcaa tgagcgccat ttaaaattaa
    11941 tcgaagaaaa tttagatgtt atcattcatg ctagaactga acgtgttcag gtattaggtg
    12001 actctgaaga agcagtagaa accgctcggt taaccattga agcattatta gtgcttgtta
    12061 accgcgggat gactgtgaat acttcagatg ttgtaacagc cttatccatg gcacaaaatg
    12121 gttcaattga taaattcgtt gccctttatg aggaagaaat tattaaggat agctatggta
    12181 agcctatccg tgttaagact ttagggcaaa aaatatacgt tgatagcgtt aaaaatcatg
    12241 atgttgtttt tggtattggg cctgctggaa cgggaaaaac ttttttagca gtcactctag
    12301 cagtaacggc actaaagcgt ggtcaagtta aacgaataat cttaacgaga ccagcggttg
    12361 aagctggtga aagccttggt ttcttgcctg gagatctcaa agagaaagtt gatccttatt
    12421 tgagaccagt ttatgatgcc ctatatcaaa ttttaggtaa agagcaaaca agtcgcttaa
    12481 tggagcgtga aatcatcgaa attgctccat tggcttacat gcgtggtcgt actttagatg
    12541 atgcatttgt catattagat gaagcgcaaa acaccactat aatgcaaatg aaaatgttcc
    12601 taacacgtct tggcttcaat tctaaaatga ttgtaaatgg cgatgttagc caaattgact
    12661 taccaaagaa tgttaaatct ggtttgattg atgctgttga gaaattacga aatatcaaaa
    12721 agattgactt tattcattta tcagcaaagg atgttgtaag acaccctgtc gttgctgaaa
    12781 taataaatgc ttattcagat tcagaaagta gtcacaagct acaatctgat aaggactgat
    12841 gtttgccact aggagacgtt gttagagagt gtgtcaatat gtaaaaaacg cctaatgctt
    12901 taggcgtttt tatgatggtg tacttcaact taacctataa agtgttgaaa attttcttta
    12961 agcatgacgt cgagatgcat tattatatcg tttaatgttt ctttaacagt gtgttcgttg
    13021 gagttgtgat gacatagttt aatgatattt tcttcgaaat gtttaggaag caatgtagca
    13081 ttcttcttag cataggaaat cattcttttt tcgccaggat gtgtcaactt attaagtgca
    13141 aaaataatat caaagtagga atctaagaaa gcagtagttc tatggtgagt actaacaaag
    13201 tcttggcgtt taagggcttt tataatttgt ttatcgtaag agggaagctt gccagttaat
    13261 aagttacgat tttgaatgat aatttgtttt tgtaactgac tgggataaga aacttcgaat
    13321 ctctctttga gtgattgtaa ttgattttca ggatcataga gtatttggca attgatgagg
    13381 ttatgccaaa aacaagtagt gtatccaatt cgagaattgt ggtgttcaac gacatcttct
    13441 aagtctgata aaaagttatc tatgttacga tagagaatat caatatcagt tccgtcgttt
    13501 aaagtaccat tatcttcaag ctcccagtaa tgattgttaa gttcaatgta atgacagtgc
    13561 ttgttaagga tacgtttacg actagtgata tcaggagttg ctgcacaata aacatacaaa
    13621 tcataatcgg aagattggtc aaaactgtca ccagagcgtg agcctccgag tgctatagca
    13681 acaacatttg gaagtttaga gaaatcttgc cagagttttg tgaaatggtc cataaatgct
    13741 ctcctaaaaa tgtaaaatat tgatgtgtct ccattctaca gggctatctc tttttagtca
    13801 ataattaata gtaagttaat aaaagaggta acggttctaa tattcaagaa gtgtatatga
    13861 gtgatttgaa aaggagatat tttatgctac aatacgtaca tattagttta aacaaggtga
    13921 tagaatgaaa caagattata tttcctacat taggtcaaaa gttggacatg agactatctt
    13981 tttaacatat tctggtggta ttttgacaga tggtaaggga cgtgtcttac tccaattaag
    14041 agctgataag aattcttggg gaattattgg tggatgtatg gagttaggtg agtcatcagt
    14101 agatacattg aaaagagaat ttttcgaaga aactggattg agagtggaac caatcagact
    14161 gttaaatgtt tatactaact ttcaagactc ttatcccaat ggcgataaag cacaaaccgt
    14221 cggttttatt tatgaagtaa gctgtccaaa accagttaat atagaaggtt ttcataatga
    14281 ggaaacattg caattggact atttttctaa agaagacgtc aaaaatatta cgatcgtaaa
    14341 tgaacaacac caattaattt tagatgaata tttttcacaa acattccaaa tggggcgtta
    14401 aaaggatggg aaatactagg tgtgacttta aagaacttct aacttttatt gatatgttgt
    14461 taggcattct tgtagaaaca aatgttaaga aaataaggag ttctcatttg tatattttat
    14521 aattattaaa aagaaaaaca gtagcttgca ataatttagt gactgttttt ttagaaagca
    14581 taagaagtac agaaaaagaa aattatggaa tattggatgg tggggatatg ctttacttac
    14641 ggtattggaa tgatatactt aaagaaatcc agttttagaa atccaaattt ctaagtaaaa
    14701 tagcataaag gagagtagat gaatgaagat aacattacac ggcgttgctg aaacgttatt
    14761 aatcacctta tatattaggg ctaaagatgc gatggctaaa catccgatac ttaatgatca
    14821 aaaatcctta gcaattgttg aacagataga atatgatttg gataaattcg ataattcaga
    14881 agcttctttt tatgcaacat tagctagaat tcgcgttatg gatagagaaa tcaaaaaatt
    14941 tattagagaa aatccaaata gtcaaatcct ttcaattggt tgtggacttg atacaaggtt
    15001 tgaaagagtc gataatggac aaattaggtg gtataacctt gatttgccag aggttatgga
    15061 gataagaaaa ttattttttg aagagcatga aagagttact aatatagcaa aatcagccct
    15121 agatgaaact tggacacggg aggtaaatcc ccaaaatgcc ccttttctaa tcgtgtcaga
    15181 aggtgtttta atgtttctaa aagaagatga cgtagagact tttcttcata tcctgacaaa
    15241 ttcatttagc caatttatgg cacaatttga tttgtgtcat aaggaaatga ttaataaagg
    15301 aaagcaacat gatacagtaa agtatatgga tacagaattt cagtttggta tcacagatgg
    15361 tcatgagatt gtggatttag accctaaatt aaagcaaata aatctgatta actttacaga
    15421 tgagatgagc aaatttgagt taggcacact tcgctcttta cttccaacaa ttcgtaaatt
    15481 taataattgt ttaggtgtgt acgaatataa agcatctgag aaaaagtaac aaaaatctat
    15541 ttatataaag agacattcta aatgtaaatt tgtcataatc gttatagcat ttgtagcttt
    15601 gtttatttag gaattagttt tacgtaagtt aggcgctcta aagtttgtat tttgaagtaa
    15661 atactaattc ataagtcaga attttattga aatggattat aagtgtgtaa tagtgattat
    15721 tagctttctc tccctttttt aatactctta tggtataatg atgtgaaaga atagcttgaa
    15781 ggaaggaaga gaacgcactt ttattttgag aactagtttt agataaaagt gaaaaactta
    15841 ggaaagtaga tgtgtcgatt atttagaatt atgcattaca ctttatcttt tctaaacgtt
    15901 ttgggtcgtt cttactatat aagatgtacg ttgaaatgat tgatgaaact ggacaagtat
    15961 cagaagatat caaaaagcaa actcttgatt tgttagagtt tgcagcacaa aaaacaggta
    16021 aagagaataa agaaatggct gtaacgtttg tcactaatga acgtagccat gaattgaatt
    16081 tagagtatcg agataccgat cgtccgactg atgttatcag tttagaatat aagcctgagg
    16141 ttgatatttc ttttgatgaa gaagatctgg ctgaaaatcc tgaacttgct gagatgctag
    16201 aagattttga ttcttatatt ggtgaacttt ttatttctat tgataaagct aaagaacaag
    16261 cagaagagta cggtcactct tatgaacgtg aaatgggttt tttagctgta catggttttt
    16321 tgcatattaa cggttatgat cattatacac ccgaagaaga aaaagagatg ttcagcttac
    16381 aggaagaaat tttaactgct tatggactta aacgacaata atcaaaaaaa atggaaaaat
    16441 cgcaccttaa catcaagcat ggaatttgct gttacaggga tttttacagc ttttaaagaa
    16501 gaaagaaata tgcgtaagca tttggtttct gccattttag taatacttgc tggtcttact
    16561 tttcaagtat ctatggtgga gtggttattt cttttattga gtattttttt ggtaattact
    16621 tttgaaatta tcaattcagc tattgaaaat gtagttgatt tggcaagtaa ttaccatttc
    16681 tcgatgctgg ctaaaaatgc taaagatatg gcagcaggag cggttttagt tgtttcctta
    16741 tttgccgtat tggtgggatt gattattttt atacccaaga tacttgcttt acttttatga
    16801 tgagaagtat tacgaataaa ttgaatggag aaagaaaaga aatgacattt aagtcaggat
    16861 ttgtagcaat tttaggtcgt ccaaatgttg gaaaatcaac cttcctaaac catgttatgg
    16921 gacaaaaaat tgctatcatg agtgataaag ctcaaacaac acgtaataaa attatgggga
    16981 tttatacaac agaaactgag caaattgttt tcattgatac cccaggaatt cataaaccaa
    17041 agaccgccct tggtgatttt atggttgaat ctgcttatag tactttacgt gaagttgaaa
    17101 cagttctttt catggttcct gccgatgaaa agcgaggcaa aggcgatgat atgattattg
    17161 aacgtcttaa ggctgccaag attcctgtta ttttagtaat caacaaaatt gataaggttc
    17221 atccagacca acttttggaa cagattgatg acttccgtag tcaaatggat tttaaagaag
    17281 tagtgccaat ttctgccctt caaggaaata atgtgccaac cctaatcaag ttattgactg
    17341 ataatcttga agaaggtttc caatatttcc cagaagatca gattactgat catccggaac
    17401 gatttttagt atcagaaatg gttcgtgaga aggtacttca tttaacgcaa caagaggttc
    17461 ctcactcagt agcggttgtt gtcgaatcca tgaaacgtga tgaagaaact gataaagtgc
    17521 atattcgtgc aactatcatg gttgaacgtg atagtcaaaa aggcattatc attggaaaac
    17581 aaggtgctat gctcaagaaa ataggaaaaa tggcgcgccg tgatattgaa cttatgttag
    17641 gtgataaagt ttacctagag acttgggtaa aagtcaagaa aaactggcgt gataaaaagc
    17701 ttgatttagc tgattttggc tataatgaaa aagagtatta atgagtttat tttgagatcc
    17761 gcaagttagt ggggaagagt tagataagtt caccgtgaga cttgattcgc acagaaaaag
    17821 caattcacga ggataaaacc aactagtcat aattttgaga ttatattcac aaattaatta
    17881 aagggagcca aacatgcttg ttggcccctt cttaaataaa ggagagcaca tgactcaaga
    17941 ttatatttgc tacctataaa gtagaggagg tgaggatatg cataatatat taagattttt
    18001 aggcattgtt attatttctg cagtgattct atttagtata ggtagtttct acgatttatc
    18061 attaatgaaa aatatactac ttatctgttg gagttttctc tttgatctat tggtttttgt
    18121 cttcaaacaa agacaaacga cagaagtact cacatggtat caagttgtta agcaattttg
    18181 gttatttatt aagtgtacta ttcttattcc tatcttagtt gcttttatta taatgaaagg
    18241 atgtttgaca agtatatcag atatattaat ttacttttat ttacaccttg tcgttgttta
    18301 ttatacgata ggaatgattt tatcattagg tcgtatcatt tctcctgaac actctatgtt
    18361 taataaactt agaaagtgaa atgaattata tttaaaattt gttttcaaca gagcggattt
    18421 gaccatctgt tgtttgccat gcctttcata atttttcagt aattatgata gttgttctgt
    18481 tctcccaatc ataaaacttt aaataagatt ctattagttc ttgcctcact ctccttaagt
    18541 ttctggtaaa aatcgaaaag ttaccagact ttaaagggga gttttttatt tgtcaacagt
    18601 tagtagaaaa acgatatatt gctccattta gattaattat ggagataacc aaataatagc
    18661 ttgtgataag aagatacctt tgaagtttca gaaacaatct gttttccatc ctgctatatt
    18721 catctttgcc aaagttgcaa caacacaata ataacaattg cttgggcatt gggtgattag
    18781 tttactatca atagggttag agtggagttg gagtatatgg attggcatta atcttataat
    18841 gatgattctg ttgagttatg tgctaacaaa aactgagcca atcagtaact accaagagtc
    18901 atcatctaac ctcaaaaaat ggctatttgc aataggtgct tttagcgttt tattttgctg
    18961 ggcactacta gtaaaaacat ttttcccaat aaggcaaaac caggagggat taatagctga
    19021 tatgaatcag gtcaatcact actttcgagt tgtcttatta ttgactatct gtcttattgc
    19081 cccaatttaa gaagagataa cgttcaggtt gtttgtcatg agaacatttg ggaactccct
    19141 gagtggtccc ttggttgtga tcttatcttc tatactattt agctggtctc acttacatgg
    19201 tctatctgtc gtcgactttg ttgtctattt tggtatgggt ttgattttcg ctagtttgca
    19261 ccactataca aaatccattc attattcgat tggcgagcat attgtttgga atagtttgtc
    19321 atatattttt tattttctag catttttgct tgatttactc taagtaataa gattgagaga
    19381 ctaatcatta ctaagacatt tttgtcagtt taaaagagct ttttggctct tttgttattc
    19441 gtttttttaa tgaataaaat gttattattt tattgttaac taaataaagt tcgtaacttt
    19501 atatgattta tatagaatta cgagtttttt gttagataaa tatgagaaaa tatagtatga
    19561 cgaattccta aataaaaaat aatgaaagga acatgatgtt aaaagatgtg aatgttggaa
    19621 tgagtcattt attagttaga ggtgctatta ttctagtagt agggattgtt tttctgttgc
    19681 aacagattca gtaagtactt caagtgggag ctttggaata ggttatcgag aagaaaagac
    19741 ccagacgttc tctttggatc agctgtccca agtgattttg tattgcgggg agcccaatac
    19801 tgttagattt acttatacaa atagcttcta tatgatgaaa gatatttata tcgctctgga
    19861 tggtgctggt agctctttga ccactcatga tcatgctgtt cctaatgatt aaaccagatt
    19921 atataaggta tttcaaagtc attgacgata tgcatgatac catgtaccaa cagactgttt
    19981 ttcatgttca actgcaagct tggaaataca ccatgaaatc gattgatcaa ttagttgtag
    20041 atgatttgaa tcatatcagt aatgagaaaa ccactttggt taaataaaaa ggaacactgt
    20101 caaaaatggc agtgttcctt tttattttag gagagagcta agattggtca tctaatggga
    20161 tttccacatg ttcaactgtc atagtaatga caggtttgat aacacaatct ctgtctttag
    20221 ggatagtctc ctgttgcaga ggcgcctcat cgctccaaat tttcttgtca gaacaattgg
    20281 aatgtagata atttgaatca gtctttttag acatttcata tcctttccag gctcaataca
    20341 atttttgagt tatgcacctt gatggcgatc agctggaata tctttagcta atgcattgta
    20401 ttgactatcc aaaagatgat cagcatccat tattgtttta ccaacaagga aatatttatt
    20461 gctttttata ttattatccc atgttgtatc aacataatat ttaacgccat taatagtaac
    20521 aatgttccaa gcgtgatttc cacctccagc ttgaccagtt acataccata ctgataagcc
    20581 agcagcagcc atatctttaa acattactgc aaaggcttga caaacaccac gtttatcttt
    20641 gtagagtgct tcaggagcat ggatactgat accactagca gtttcacgag tggttgccag
    20701 ttcttcagtc gcgtaagtgt aattattaac caagtggtca tgaattactt tagctttttc
    20761 ataatcactt gtgatatttt tgttagtaat attttctttg acaaaagaat ctacaaactg
    20821 tttatattct ttatgtaatc ctaccatgtc acctttaaga gtataggtga ttgtgatgtc
    20881 gctttttaca ataatgcgtt ggatttgtcc accaagagta taagtgctaa tgttactttt
    20941 ggcattgaat tcagttgctg ttccaagtgt atctccaatt tcccggatga tgcttccgag
    21001 atttttttgg tattcgtcaa cttgttcttg agttttaaac tccatttgaa cactgatgtg
    21061 tggtacttgg ttgctaatat tatttcgtaa aacttctttt aaactatcgt aatcggcaat
    21121 attagctgac tcaggctgtg atgcctttgc atcagaggta gcctgttttt cctcactctt
    21181 agttacttct tgtttattgc ctgacgcttc tgattgagta gttttttctt ctactttgtt
    21241 tgcaggaaca gaagtatttt cgctaacttt gagagtagca agtttagaag tctcagtttc
    21301 aatagccgct gttgataggg catctacttc ttgttgaagg attgccatag cagctttgta
    21361 attattaact tcagctacaa gaggttcaat ttgatttctg agtgaatgaa ataaattttc
    21421 agcttcttgt atagaattca cagagttgta gtttacagtt gctaaaatag cttgaatttt
    21481 gttaacaacc tgattacgtt gccccacgag ctgagctgct ttattttgac ttgtctgaat
    21541 cttattatga ttttggtcaa tcagattttg aacagtttgg ttaatgacat tacgttgatt
    21601 aacgatagca ttatagttac tgggagagag atgaccagct ttcacctggt taaataattg
    21661 ctcatctttt tgtttaaata atgttatagc tctatttaac tgttcaatag ttggattagc
    21721 atttagttga gtgaatgagt tgacattatc aacaacaaat tgattgagca aacgatgttg
    21781 ttccttgatg taactaataa gctgcttttc attattttgc ttactgatat agtgatactc
    21841 actggtggtg tcatcagctt taacctcata gttaatagtt gatagtgaac tgataattaa
    21901 agctgcggca aagccactaa taattttttt aatcttcatt agttctcctg ttataaacta
    21961 aataaaatgg gattccataa caaccatttg tggggagttt tatccaccaa cgataattag
    22021 gatatccatg attaatgtga acaacaaagc tttgttaatt tttttcatga ctatctcctt
    22081 tctgattttc tattttgtac ttgtattata taatggattt aaagggtctt ttaaattgtt
    22141 gtgaaagtgg gaaaaatgga aaaagaatta ggaaaaacac taagaagatt acgtaaagga
    22201 aaaaaagtta gtatttcttc tttagcagat gagcacctat ccaagtcaca aatctcaagg
    22261 tttgagagag gagagtcaga aattacttgt agtcgtttgc ttaatatttt agacaaactc
    22321 aacatcacta ttgatgaatt tgtgtcgatt cattctaagg cgcacactca tttttttatc
    22381 cttttaaatc gagtaagaaa atattgtgct gaaaaaaatg taactaaatt agtggctctc
    22441 ttagaggatc ataaccataa agactatgaa aagattatga ttaaggctct tattttttca
    22501 attgatcaat caatagagcc taatcaagaa gaattagcac gcttaacaga ctatcttttt
    22561 acggtggagc agtggggata ttatgaaatt attctcttag gaaattgctc acgtctcatt
    22621 aattacaata ctttattttt attgaccaaa gaaatggtta attcgtttgc ttattcagaa
    22681 caaaataaaa ccaataaaat cctagttaca cacttagcaa ttaattgtct aatcatcagc
    22741 attgatcact cttactttga gcatagccac tacttaattg ataaggttag atcattgtta
    22801 caggatgaag ttaatttcta tgaaaaaact gttttcttat atgtaactgg atactatcat
    22861 ttaaaacttg gtgatacttc ttcaggaaaa gaagatatga ggaaagcttt acagattttt
    22921 aaatatttag gtgaagattc attttacaat acttataatg aacattattg tcaaaaagta
    22981 cttggcaata ataaagaatg ctaatgagtg caagtttatg agtaattttt aaagcttttg
    23041 tctcaaaatg ataataagaa aggaaaatag aaatcgaatg gtcaggtgac atcaagaacg
    23101 tttggaaatt attgctctaa gcaatgacta ttgattttta gactatatat ttatgccaga
    23161 attaccagaa gtagaaaccg ttagaaaagg actagaaaga cttgtagtta atcaagaaat
    23221 agcttctata acgattaaag tccctaaaat ggtgaaaact gatttgaatg actttatgat
    23281 aagtctacca ggaaaaacaa ttcaacaggt attgagacga gggaagtatc ttctttttga
    23341 ttttggtgaa atggttatgg tatcacattt acgcatggaa ggaaaatatc tactcttccc
    23401 aaataaagta ccagataaca aacattttca cctttatttc aaattgacta atggcagcac
    23461 attggtttac caagatgtcc gtaaatttgg gacatttgag ttggttcgta aatcatctct
    23521 caaagattat tttactcaaa aaaaattagg tcctgagcca actgctgaca catttcaatt
    23581 tgagcctttt tcaaaaggct tagcaaactc caaaaagcca ataaaacctt tactattaga
    23641 tcagagatta gtagcaggac ttggaaatat ttacgtagat gaagtcttgt gggcagcaaa
    23701 aattcaccca cagcgtttgg ctaatcaatt gaccgaatct gaaacaagct tattacacaa
    23761 ggaaattatt cgcattttaa ctttaggcat cgaaaaaggt ggctccacga ttagaacata
    23821 taagaatgct cttggagagg acggtacgat gcaaaagtat ttacaggtct atggtaaaac
    23881 tgggcaaccg tgtccaaggt gtggttgttt aattaagaag ataaaagtag gagggagagg
    23941 gacgcattat tgccctcggt gtcaatgctt atgacaaaaa taataggact gacaggaggg
    24001 atagcttctg gaaagtcaac ggtaacaaaa ataatacgag aatcaggttt taaagtcata
    24061 gatgcggatc aagtggttca taaattgcaa gctaagggtg ggaaacttta ccaagcttta
    24121 ttagaatggt tgggtcccga gatacttgat gctgatggtg agttggatag accaaagctt
    24181 tctcaaatga tttttgctaa tccagacaat atgaagacat cagctaggct acaaaatagt
    24241 atcattcgtc aagagttagc atgtcagcgc gaccaattaa aacaaacaga agagatattt
    24301 ttcatggata ttcctttatt gattgaagaa aagtatataa aatggtttga tgagatttgg
    24361 ttggtatttg ttgataaaga aaaacaatta caacgattaa tggcccgtaa caactacagt
    24421 cgagaagaag cagaattacg actttcacac caaatgcctt taacagataa aaaaagtttc
    24481 gctagtctta ttattgacaa taatggtgat ttaataactt taaaagagca aatattggat
    24541 gctcttcaac gtttatagaa ctactaaatg gataatgtat ttatccattt tttgtcttta
    24601 ttacactagt tttaaaaaac ttgcgattaa accacagtaa tagtacaatg atataaaagg
    24661 tttactttca aaggagtttt tatggagtta gtaattagag atattcgtaa gcggtttcag
    24721 gaaacagagg tcttgagagg agcaagttac cgattttatt caggtaaaat aacaggggtc
    24781 ttaggtagga atggtgctgg gaaaacaact ttatttaata tactttatgg ggatcttgca
    24841 gctgacaacg ggaccatttg tttattgaag gataatcacg agtatcctct taccgataag
    24901 gatattggta ttgtttattc cgaaaactac cttccagaat ttttaacagg gtatgaattt
    24961 gtaaaatttt acatggattt acatccttca gatgatttaa tgacaataga tgattattta
    25021 gattttatgg aaataggaca aacagagcgt catagaatta tcaaaggata ttctgatgga
    25081 atgaagagta agctctcatt aatttgcctg atgatttcta agccaaaagt aattttacta
    25141 gatgagccac tgactgcagt tgatgttgta tcaagtattg caataaaacg ccttttgttg
    25201 gaattaagtg aggatcatat tattatatta tcaactcata taatggcctt agcagaagat
    25261 ctatgtgata ttgtggctgt attagacaaa ggaaaactcc aaacattaga tattgatcgt
    25321 aaacatgaac aattcgaaga gcgtcttctt caagtgttga agggagatga atatgacaag
    25381 taaagcatta atatggtcta tattacagat tgatagtgct agagcttata atagtttttt
    25441 atattacctt cagaaaatcc cctggttggg aagaaaaatc cctaaccgat ggtataaaag
    25501 gtgtagtact atttattcgt atcattttct ttttcttaag aggttttggt cgttccatag
    25561 gttacgtact atattgttat gttttatcat tttttgttag aagtggcatg aagcagttaa
    25621 atattttacc gcaagaaggt tttaccctaa ctttaacatt gatggtgatt acctctcttt
    25681 taattgtcat aaatttctat ataaaactta tagaacctaa tgatatgcaa caaactcata
    25741 tgattcgcct tttcagaatt gagccatcga ggtatttcca atccgctgaa cttttagaag
    25801 ccagtagtga actatttttt agaagtttga gctttggcat atttttccat ttaaatcact
    25861 ttgctttttg gcatggtttg acttttgctt tatttttagc aggtagtcgc cttggaatca
    25921 aggtgctctg tttaccttta tatggtagag gtaaagacca agagaatcca gagtctttac
    25981 taaatatttt attttactta gggattggta taggagtctt cctttctctc tttttccttc
    26041 ttcttggcaa acagttctct ccatatccat ttttttcatg gtacaccggt attgcaggac
    26101 ttatcatatg gttagttagt tactatcgat taaaaactta ttctaaactt aaccacttga
    26161 cctaccttac cctaacgcat gagacgatga aggagaaaat tgcaaagatt gataatatag
    26221 agctgcttgg gattgaaatg aaagataggg atataaatgt cagtgaactt tctcccctgc
    26281 tatttgaaaa tttatcaggt atttcatatt taaacgctat ctttttaaaa cggatgagta
    26341 gatacttgca aaggaaaatt ttggtacgtt tagctgtttt aagtgttctg tttggaatag
    26401 aactcctttt cctgctattt tttaaagaaa agataaatat tccgatgaat gagcatagtt
    26461 tttcaaaatt cctatttttg agtgcagttc caggatatct aatatatatc ggtgaatcct
    26521 attcaaaatt ttgtttctat catttggatc gtccattatt aagatataat ttctatcgtg
    26581 aaagagaaaa tatcttagca acc
//
LOCUS       DATRSG010000023        25422 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRSG010000023 DATRSG010000000
VERSION     DATRSG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25422
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.023"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(276..890)
                     /locus_tag="VBD89_000946"
     CDS             complement(276..890)
                     /locus_tag="VBD89_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7135489.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(1023..1448)
                     /locus_tag="VBD89_000947"
     CDS             complement(1023..1448)
                     /locus_tag="VBD89_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7135490.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(1575..5981)
                     /locus_tag="VBD89_000948"
     CDS             complement(1575..5981)
                     /locus_tag="VBD89_000948"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO7135491.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQNLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(6104..6688)
                     /locus_tag="VBD89_000949"
     CDS             complement(6104..6688)
                     /locus_tag="VBD89_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO7135492.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(6813..8666)
                     /locus_tag="VBD89_000950"
     CDS             complement(6813..8666)
                     /locus_tag="VBD89_000950"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO7135493.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(8758..10017)
                     /gene="rseP"
                     /locus_tag="VBD89_000951"
     CDS             complement(8758..10017)
                     /gene="rseP"
                     /locus_tag="VBD89_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO7135494.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(10048..10842)
                     /locus_tag="VBD89_000952"
     CDS             complement(10048..10842)
                     /locus_tag="VBD89_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO7135495.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(10857..11609)
                     /locus_tag="VBD89_000953"
     CDS             complement(10857..11609)
                     /locus_tag="VBD89_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO7135496.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(11776..12117)
                     /gene="yajC"
                     /locus_tag="VBD89_000954"
     CDS             complement(11776..12117)
                     /gene="yajC"
                     /locus_tag="VBD89_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO7135497.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(12194..12538)
                     /locus_tag="VBD89_000955"
     CDS             complement(12194..12538)
                     /locus_tag="VBD89_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO7135498.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(12729..13892)
                     /locus_tag="VBD89_000956"
     CDS             complement(12729..13892)
                     /locus_tag="VBD89_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO7135499.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(13917..15254)
                     /locus_tag="VBD89_000957"
     CDS             complement(13917..15254)
                     /locus_tag="VBD89_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO7135500.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            15408..16934
                     /locus_tag="VBD89_000958"
     CDS             15408..16934
                     /locus_tag="VBD89_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7135501.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYKISLKQNKVIIRTNIIYSEGS
                     "
     gene            16936..17616
                     /locus_tag="VBD89_000959"
     CDS             16936..17616
                     /locus_tag="VBD89_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7135502.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(17637..18632)
                     /gene="galE"
                     /locus_tag="VBD89_000960"
     CDS             complement(17637..18632)
                     /gene="galE"
                     /locus_tag="VBD89_000960"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO7135503.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(18720..20327)
                     /locus_tag="VBD89_000961"
     CDS             complement(18720..20327)
                     /locus_tag="VBD89_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO7135504.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(20456..21589)
                     /gene="ugpC"
                     /locus_tag="VBD89_000962"
     CDS             complement(20456..21589)
                     /gene="ugpC"
                     /locus_tag="VBD89_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO7135505.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(21690..22541)
                     /locus_tag="VBD89_000963"
     CDS             complement(21690..22541)
                     /locus_tag="VBD89_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7135506.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTQSLEEASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(22636..23532)
                     /locus_tag="VBD89_000964"
     CDS             complement(22636..23532)
                     /locus_tag="VBD89_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO7135507.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGEPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNYALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(23586..24563)
                     /gene="lacD"
                     /locus_tag="VBD89_000965"
     CDS             complement(23586..24563)
                     /gene="lacD"
                     /locus_tag="VBD89_000965"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO7135508.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(24565..>25422)
                     /locus_tag="VBD89_000966"
     CDS             complement(24565..>25422)
                     /locus_tag="VBD89_000966"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO7135509.1"
                     /translation="NRVTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGHFGDFIRS
                     GLDALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEAGAFLNHLKLIFDA
                     ATIITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPN
                     LEELTQLIGKPISYSLDELKSTLQQDIFRGIDWVIVSLGARGAFAKHGNHYYQVTIPK
                     IEVINPVGSGDATVAGIASALEHQLDDINLLKRANVLGMLNAQETLTGHINLTYYQEL
                     ISQIQVKEV"
BASE COUNT         8117 a         5198 c         4033 g         8074 t
ORIGIN      
        1 ttacaaaaag tctaaatttg aatgaaacat tctattcaca attaaaagaa ggagaaatga
       61 aactaagtga aaatcccgaa aaaggagaaa cgaacacgaa ttaacctttc aaatatctac
      121 atcaaataac agaagtctcc gtatcaagcg aattgtagct tttttgaata tactaaaaac
      181 actcctacct ctattttact agaagtagga gtgttttttt cattttagtt catagatgca
      241 gtgccattgc tatccttgcc tttttttgta cagtattatc cgatgacttt aaagcggtca
      301 tcatcattca tgaattcttt ttctcctgtt tcagcttcga ttgaaccaaa atttagttgt
      361 ccacgaagtt tccaactagc tggaacacca taaacagctt gtactgatgc atcaataact
      421 ggattgtaat gttgtaaatt accaccaagt ccaagttctg cagaaagggc tgtccaagta
      481 tttactgagg caatacctgt tgattgctca gaccaaactg gaaagttatc cgcataaaga
      541 acgaattgtt cttgaagaga tttgacaaca tcttgatctt caaagaagag tactgttccc
      601 tttgaagctc caaaactagc taatttttcc ttagttccag caatagctgt ctctggagca
      661 ccttgtactt tcattgtttc taccaaatca ttagcaacca attcatccca gaacttagta
      721 acttcatcat tcaataaaat gacaacacgg gaagtttgac tattaaaagc agatggggat
      781 tggcgaactg cctctttaat aatctctact attttctcat cagaaacttc tgtattgcga
      841 ccaagagcgt aaatagagcg acggtttttt aattcttcca aaaatgacat attttttctc
      901 cttgttcttc aatctcagat atcttactac atttgttcta gtgacatatg aattagatta
      961 aagttattta ttactatgta gtaattatat taccactata tcgtaaagtc aaagaatgtg
     1021 actcacaagg ttttaaattt ttttaaaagg ttaatcaagt cttcttgctc tgtttctgtt
     1081 aagactgaaa aagcatcttc tacacgttta atatgctctg gtagagcttt ttttatcact
     1141 tcttctcctt cagttgttaa actcaccaaa aaggctctct tatcattcgg acaagaatgg
     1201 cgtaataccc atcccttctt ttccatattt ttaataacaa cagtcatatt acctgatgta
     1261 gcaagcatat tttcaatcaa ttctccaatt ttcattgtgc ctttagcata gagaacatcc
     1321 aaaactgcaa actgagttgg tgttaattca aattctttaa atgattctga gacttttccg
     1381 tctattgttc taaatgcttt tcgcatgaca accatagatt taaccgctga atttttaaag
     1441 tttcccattt catctcccaa acgtattttt tcttatttta tcactaatta gttattttaa
     1501 gcaagaatat catctttgcc ctatcaaaag cagctggcaa ataataaggt tgagtaacta
     1561 ctcaacctta ttgcttagaa gaaatcatca aaaagactca actgattgtc ttctggtaaa
     1621 tttcccaaaa tacccatctc atcaaatttt tcaactaagg tacttgagac tcctcctcgt
     1681 ttacgaagct ctgtttttga taaaaattca ccatcttcac gtgctcgaac tatctgtttt
     1741 gcaacgtttt ctcccaagcc ttccatagca acaaatggag ggattagcgt atcctcttca
     1801 ataataaagt cagtcgcatg gctacgataa agatctaatt tcccaaattt aaagccacgc
     1861 tcaagcattt cattaaccaa ctctaatgtc gtaaaaagat cattttcgac attcgtcgcc
     1921 tcattacgct gacgcttctc agtgatatcc ttcatgcgcg ccttaacggc atcaagtcca
     1981 gcactcattg tgcgtaattc aaaagctttg gcgcgaatag aaaaataagc acaataataa
     2041 aatattggat aatgtacttt aaagtacgcc acacgtaagg ccattaaaac ataggctgct
     2101 gcatgggctt tagggaacat atacttgatt tttccacacg attcaatata ccaatccggg
     2161 acattattat cacgcattgc ttggatataa ccgttacgct catcttctga aattttcaac
     2221 cataacccct tacgcacgcg ctccatgatt gtaaaggcca tctttggttg tagaccagcg
     2281 tgcattaaat aaaccatgat gtcatcacga caaccaataa cggttgataa ggtagcaata
     2341 ccttctttga ttaagtcttg agcattacct aaccaaacat ccgtaccatg tgatagtcct
     2401 gacagttgca acaattctgc aaatgtggtt ggatgggttt cgttcaccat accacgaaca
     2461 aagttcgtac caaactcagg aataccaagc atccctgtag gagttccaat ttgctcttct
     2521 gtcactccta aaacttccgt accagaaaat aacttcataa cgtctggatc atctggaagg
     2581 atatttgatg gatcaattcc tgataaatcc tgaagtttac gaatcatagt aggatcatca
     2641 tgtcctagaa tatctaactt caagacattc tcatcaatat catggaaatt gaagtgagtt
     2701 gtctgccaag cagctgtcat atcatctgct ggatattgaa ccggagtaaa atcataaaca
     2761 tccatatagt taggaataac aacgatacca ccggggtgtt gacctgtaga acgtttgact
     2821 cccgcagcac cagtcgctaa ccgttcgacc tctgcatcgt tataaaattt attgtaatct
     2881 cgttcgtaac ctttgacaaa accaaaggca gttttttcag ctacggtacc taccgttcct
     2941 gcacgaaaag catattcttc tccaaagata tcacgaacat ctaagtgagc agaaggttga
     3001 tcatctccag agaaatttag atcaatatca ggaaccttat ctccatcaaa tcctaagaaa
     3061 gtttcaaacg gaatatcctg tccatctttc ttgtacaaag taccacattt aggacaattt
     3121 ttatttggta aatcgtaacc tgaaccacaa gaaccatctg tgataaattc agaatgctga
     3181 caatttgggc aaacataatg aggtggcatt ggattaactt cagtaattcc tatcatggtc
     3241 gctacaaaac tcgacccaac agacccacgt gatcctacta gataaccacg ttcattagaa
     3301 cgttgcacca acatttgcga agcaagatag ataacggcaa atccattacc aagtatagat
     3361 gctaactctt tttcgatacg taaatcaata atatcaggaa gaggattacc atatatttcg
     3421 aatgctttag cataagttaa ttcagcaact cgttcttcag cacgatcaac aaacggggta
     3481 tagagatccc cttttacaac ttcaacgtct tcaaatcgat ctgcaaaagt attcgtattg
     3541 gttacaacga tctcataggc taaatcttta ccaagaaagg caaattcatc taacatctca
     3601 tttgttgtac ggaaatgtgc ttttggcaaa ggcgctggtt gagcatcctc cccacgaccg
     3661 atagtcctat taatcatggc accttgtcca agactacgaa caataatctc acggtaaatc
     3721 tcatcttcag gctcaatata atgaacattt cccgtagcta aaacaggctt atctaaacgc
     3781 cgaccgacct ctattaggtc tctaataatt tgttgaatcc ccgcctcatc tttaattaaa
     3841 tcacgaacca ccaaggggcg ataaatagca ggtggcatga cttcaataaa atcataatat
     3901 ttagctaggg tcactgctgc atcaattcca tttgataaca aggcatcaaa aacttctcca
     3961 tcagaacagg cagtacctag caagagacct tctctgtgag catctaaaac tgatctagga
     4021 attcttgcca ccccttcaaa atatttaaca tttgaaaggc taactaactt aaatatattt
     4081 ttaagtccaa cctgattttg gacataaatg gttgcatgct ttatgcgtgc ctttttgtaa
     4141 gagtcttcag ctaccaattt agtattgagc tccatcaaat tagtcacatc acgattttcc
     4201 cgcgcctctt ttaagaaaat aaagagtaag cgacccgttg cttcagcatc gtagttggcc
     4261 atatgatgat gttccaaagc aacttggaaa cgtttggtta gaggccccaa accatgacgc
     4321 ttatattcag gataaagatt tcttgcaaat tctaaggtat caatgaccgg ctgtgtgatc
     4381 aaaggcagat tatgacgttc ataattcgca ttcataaaac caacgtcaaa ggttgcatta
     4441 tgcgcaacta aaaccgtacc ttgacaaaaa ttctgaaatt cctgaaggac ttgtaaaata
     4501 ggttttgaac ctctgacatg attatccgta atacctgtca actcagttgt aaagtcagaa
     4561 agaggatggc ctgggtcaat aaactcatca aattgctcaa taatattacc cttgaacatt
     4621 tttgatgctg caatctgaat taggtcatta ttagctgcag aaagacctgt agtttccaca
     4681 tcaaatacaa cataagttgc ttcgtgcaaa ttcatatcaa cttcgttgta ggaaataggc
     4741 actttatctt caacaatatt tgcctctaat ccaaaaattg cttttatacc tgcttttttt
     4801 gctctgtggt agccgtgcgg aaaactttga acattggcat ggtcagtaat tgcaatagct
     4861 gggtgtcccc acttagctgc agtatcaatg aggctctcga cagttggtaa agcatccatc
     4921 gttgacatat tggtatgagc atgaaattcc acacgttttt gatctgctgg cattaagtct
     4981 ttacgttcat gatgcacaat ctctttgata tcttgaacat tcattgtcaa agatttagta
     5041 aagttattgt tctcaatatt ccctctgacc cgcaaccacg aacctttaga aatcatatcg
     5101 tacttcttca attcttcatc atctttagcc cacttttgca tagcaaaact tgaggtataa
     5161 tcagtcatct taaaattaat gatatgacgt ccagtgcggg ttgtttttcg ttcaacacta
     5221 aaaaccattc cttcaaaaac gatacgattt tcttctgtgg tcacctcaat catgggtgta
     5281 atctctgctt tttcaaagcc agcttgacgt tgtttgatac gctctttgaa atcataattt
     5341 tgagttggtg tcacttcttc tgatggaggc gctgaatctt ctagtgattt ttgtgcttcc
     5401 aaggcttgca tagcctcttg gttagctttt tcaagcaatt gctcgcggtt agtttcaaat
     5461 gatgatttta agttttgcgt catttgttca tcagaaacca tatcaatagc aagtttacca
     5521 aatccaaaca atgaaaattg ttgttccaag cgcggcaaat gattctgacg aaaatgatta
     5581 ttattgacaa attgaggtgc tgaaataatc atctgactgc cattgtaatg aacttttaat
     5641 tgtgaaaaag aactcttaaa acttgcacta ttacacaagg gttcacaaaa agcttgttga
     5701 taataatcct gtagcagatc atcagaaaaa tcaatagttt ctgctcttat atcaaaagta
     5761 gcctttatat ctgctttttc aaaactattc actaaacgag tttgtaattc acaatacacc
     5821 tcaattggca ataattctgg aaatgaaaaa tgaaattccc ataagcgtga tagagaatga
     5881 acctttacct cgataatatc agcagacgaa aaaacacttg aattcttaat ttcaagcggc
     5941 atttcaatct gatccataag tttcttgaac aattctgaca tagttttctc ctgaatatct
     6001 tacttccatt ttatcacata tctgtaaata tttcgagata acaaaaggag gctgttaaaa
     6061 aaggcattta ttagccttct taacaacact ctcttaactg agactattta tcataagcat
     6121 ctagactgta ggtttcaata atttggttta atttactagc ataagcagta tctgtcgcat
     6181 aaagacctgt tagagctgca gtagcatcct tataagaaga agtatttgat ttccaaacag
     6241 atgcatactt ttgactagat actaactcag cataatcata tagtgaagca gaataatttg
     6301 gataggcgcg aaaaggagct tggatttgag tcatattgcc tttcccatca tcttctaaag
     6361 taggcatttg gacagattta cctttatatt ctcctttgat gccaaagagg ttataattag
     6421 gagccttaga caaatctgat tgtccactgg atgattccaa aatagcttgt gctaataaga
     6481 ctgacgcata caaatcatag gtcttagaaa tttcaatagc tgttggagaa atcgtttgaa
     6541 taaaagtcgt cgtttgatcc gatgcactag tgactgtcgg ttcgacatta tcatttttat
     6601 aattgtaaaa ccaaacccca cccaaaccaa aaactaatag tgttgttgtt aacctcaata
     6661 ccaatttatc tttttttcga cttttcatga tggctccttt ctaaaatatc atttcattat
     6721 aacaaaaggc agttgctttt taccaataag aaccatacta aatttcactc catataaaaa
     6781 agttagcaca tcagactaac ttttttatcg gcttattttt ttgttagaat ctctaaagtt
     6841 tcaattaagt tatcagcgtg aacctcaatc gtatcaccac ttgctttaat tttcacttca
     6901 acgatacctt cagaagcttt cttcccaaca gttactcgaa ttggaagtcc aattaagtca
     6961 ctgtctgaga atttagaacc aacacgttca ttacgatcgt ctgtcaggac ttcataacct
     7021 ttaagcatca aatcagcttc aattttctca gtaagatctt gtgactcttg atctttgaca
     7081 ttgacagtaa tcaaatgtac atcaaatggg gctaattctt ctgggaaatt aatcccccaa
     7141 gcaaagcgat aagctccttt tggtgtctta ttcacaaaaa gtcgggcatg ttgttcaatg
     7201 acagctgaga gaatacgact aacaccaata ccataacacc ccataacaat aggatttgag
     7261 cggccatttt catcaaggat gtttgctccc atactatctg aataacgggt acctagttta
     7321 aagatatgtc caatttcaat accacgagca aatttcagag tacctttacc gtctggagaa
     7381 atctcccctt cttttacttc acgaatatca acgtattcag ctgtgaaatc acgttctgga
     7441 ttaactccag taaaatgata accatcttgg ttagcaccag acacagcatt agcaaggtct
     7501 tgaactttac gatcagcaat aattttaaca gactcaggta ggttaactgg cccaagagaa
     7561 ccaaaacctg caccaaaaat ctcttttgct tgctcttcac ttgcaggctc taagaaatca
     7621 gccgctaaat aattttttaa cttcacatca ttgacttgat cgttaccaac tagtaaagca
     7681 accacgggct gttcatcagc aataaacaat agtgtcttga tagtttgatt ttcatcaatt
     7741 gataaaaatc cagctacctc atcaattgat ttgcagttag gagtttctac ttttgttact
     7801 tcttcaaagg tagcagcctt agtagatggt ttgtactcat tcgttgccat ctcaaggtta
     7861 gcagcatagc ttgattcagt ggagtatgca atagtgtcct caccagaaac tagccaagcc
     7921 gagagttctg ccttaatttc ttctaaaaca tcctctggaa tatcatcaat agaaggaata
     7981 gttttatcaa ggactaacca acgatttaaa tctgtacgat tgggagttac tgccataaat
     8041 tcttggctat ctttacctcc catagcacct ccgtcaccta taatgccttt aaagtcaagt
     8101 ccagcacgtg tgaagatggc ttcatatgct ttacggtaat cttcgtaagt gacatcaagg
     8161 tcttcataat ctttgtggaa gctgtatcca tctttcatga taaattcacg tgttcgaaga
     8221 agtccattac gaggacgctt ctcatcacga tatttagact gaatttgata aaggtttagt
     8281 ggtaattgct tataagattt tactgcatca cgaacaagag ttgtgaaagt ctcttcatga
     8341 gttggtccta agataaaatc cgattgatca cgattcttta atttataaag gtcttcacca
     8401 tatgtttcgt agcgcccaga ttcacgccat aaatctgctg taagaagagc tggagctaac
     8461 atttcaactg caccaatctt ctcaaattct tgacgcataa ttgttttaaa tttttcaata
     8521 gtacggttag ctagaggcaa ataagcatag attccagccg aaacttggcg gacatatcct
     8581 gcacgaacca taagagcatg gctgataacc tgagcatcac ttggcatttc gcgaagtgtt
     8641 ggaataagca ttttactttg tttcattaca ataagatacg actaatcaaa gttgattctt
     8701 tttcaattga gaattgcaat tgaaaaattc gtatttcctt tcacgtttta ttatctttta
     8761 gaagaaagct cgcatgatat cgttccaggt tacagcaatc attaatacta acataactgc
     8821 taccccagcg agcgtaatat aagtttctgt ttcttgttta agtggtttgc gacgaatggc
     8881 ttctaaaata ttcatcacta tcttaccccc gtcaagggct ggtataggga tgagattcat
     8941 aatacctaag ttgatagaga ttaatcccat taagttgaga actgtgacga aaccattttt
     9001 agcagcttgg ctactagcct gatagagagc tactggacct cccaatttat taatactaaa
     9061 atgagcaata agtttcttta attcatttaa aattctaaag aaactttccc atgctagttt
     9121 aaatccacct aaaagcttgt ctttaaagct tgtctttaga gctggcataa tgccgatgat
     9181 gtaggattta tcaactttct gtggcttaac gttgatcgtt ttcaccacct ctttgctctt
     9241 aattttaagg gcaagttttt ggtttttttc taaatgacta gttgactttt ctactgctgc
     9301 tgtcaactgt tcccagttag aaactttgtg cgaccctatt tgtaaaatac ggtcattatt
     9361 ttttaaacct gcactagcag caggaccatt ttctgataca cggacctgat tggttgataa
     9421 atcttgaaca cctccttgaa taaaagcaag tgcaataaag actacaagac ctaaaataaa
     9481 attattcatc ggacctgcaa aattggtaat taagcgcccc catacagaag cattctgata
     9541 ctgcacatct aagggagcaa tacgaatttc agtaccatcc tcctcaatga tagtagcatc
     9601 gtgatcaaca ctataggttt ttgtctcact taagaccaat cctgtgatag ttagtttatc
     9661 ttctaaatca taagctgtta cattgattgg taaagaagta ttgtctaatt gttttcctga
     9721 taaattaata cgagttacaa tgccttcttt atttaaagta aggctagccg gcgtccctgt
     9781 cttaatttca gtcttatcat caccccaacc agccatacgg acataaccac ctagcggtaa
     9841 tatacgaata gtataggttg ttccttcttt atcaatgtga gaaaagattt ttggacccat
     9901 accaatagca aattcacgaa caagaattcc tgatttcttt gcaaaataga aatgcccaaa
     9961 ttcgtgaaca actacaatga caccaaaaat aataataaag gttaaaattc ctagcattca
    10021 attcctctct aaatacaata tattatttta gaataagcca aagaagtgca tgattgggaa
    10081 aacaaaaatc atactatcaa agcgatccaa aatcccccca tgacccggaa tcaatttacc
    10141 cgaatcttta acaccaaaat gacgtttaat actactttca actaaatctc caaattgacc
    10201 aaaaatgcta aagatagcca ctaaaactaa catgactagg aagctatgag gcgcatagac
    10261 ggtcttatca aaaagcataa aaaagaaagc taccacaatg gctgaagcaa ttcctcccag
    10321 actcccttca attgttttat ttggagacac tgaaggtagt agtttgcgtt gtccaaattg
    10381 acgaccaatc atataagcac cgatatcagt cgcccagaca ataaataaag ctaacaacac
    10441 cttatctatt ccagccatac gagcactgac tagattttga aagccaatac ccacataaaa
    10501 actagacgca attggaaagg cagcatcttc aaaactgtaa gaattactgt tcaaaacagt
    10561 acctgccaaa atcataaaaa taacaatacc gtatgctgat aaactagcgt caactggtaa
    10621 aaaggacaaa taactatcta aaggaactgt taaaacaaag gctcctatca tagctaatgc
    10681 accttcaaag gaaaatattt ctaaacgacg catacgaagt aattcagaaa caccaatcat
    10741 agctaaaagt ccaactaaaa attggaaagg caaaccaccc attactaaaa aagggataaa
    10801 tatggcaagc gcgacagctc cccaaataac acgttctttc attttctctc ttctttctat
    10861 acactaccaa atcgacgatg gcgttgatta taatccacaa tggccttatg tagttcgtct
    10921 tttttaaaat ctggccataa aacgggagta aagtaaaatt cactataagc tgattgccaa
    10981 ggtaaaaagt tactcaagcg taactctcca ctagtgcgga taatcaaatc agggtcccta
    11041 taaagatagg gtaagctatt tgtcattaaa tgatttgcta ctaaatcctc tgtaatatcg
    11101 tcaggattca acttagcttc caaaacatcc tgagcaattt ccttaatggc attagtgatt
    11161 tctgaacgtc cgccataatt tagcgcaaaa ttcaatacga gccccgaatt atgttttgtc
    11221 ctcaaacaag cgcgttgcat tgcatcgtat gttgccttag gtaatttatg agtatctccg
    11281 atgacctgta cacgtacatt atttttatct aactctggta catatttatc aaaaaattca
    11341 accggaaggt tcataataaa cttaacttca tcatctggac gtgaccaatt ttctgtagaa
    11401 aaggcataga cagttagtac tttaacacct aattctgaag ctgctattgt aacttcttga
    11461 agagcatcca tccccgcctt atgtcccata acgcgtggtt ttaaacgttt ttttgcccaa
    11521 cgtccgttac catccatgat aataccaata tgtgctggta ttttctccaa aggcatttct
    11581 acttgttttt ttcttttaaa tataaacata atgttcctta atttagcaat cgtttttctt
    11641 atcagccctt ttaaaagagc tggaaatatc ccatactata acattttacc acattttaac
    11701 ttatttttga gtattaaaat gccatttgtg acagcaaact aaaaagacca gaacaatcat
    11761 ctggtcctac atatcttatt cagtttcgat ggctgtttct ttattttcgt cttcattgac
    11821 ttcaataatc ttaacatctt caactggttc tgttggtgtt gctgccttag agacaacaga
    11881 tttgatagct gccaactcaa acgtcaagta gacaccatca acatctaaaa ccattcgttg
    11941 tgcttcagta ttaacttcat caactactcc aaaaagacca ccaattgtga caatttcatc
    12001 acctttttga acagcattca attgtttttg acgttcttgt gcttgtttct tttgctgacg
    12061 ttgcatgaag aacatcatac caaccataac aacaagcata ataataggta agctcatttg
    12121 attctccttt ccgtgttcta cgtaatcatt actatagcag atttatgcta tatctgcaag
    12181 gagatactgt ctcttagcca ataaattcac ggatttcttc ttttgtcatt tttgaatcgc
    12241 acttcacctt gatagttccg tttttaacaa ccatcaaacc tggcactgta ggaatgtcat
    12301 atttatttcg aaaatcttta tttgcagtcc aatgatcaaa atcttcacta tctaaatgga
    12361 ataattcaaa ttcatcttca tcagctactt ctacaatttt aggaacaaag aggtgacaaa
    12421 atggacaaga aatacgacca atgaacagta caaaatcttg atttgaatca attttatctt
    12481 caatttcggc agccgttatc aaagtcatgg ttgccaaaag ttcttcaaaa gaatacatag
    12541 ttaaccaact ttctaaatct tatatttaaa gaataaccta tgatggcaaa acttgcaagg
    12601 caagagtaac aacgattcta tcaaaatcat taactcactt ctcgcttcaa actatcctca
    12661 aaaatgctca ctcatattta aatgactcat caaaaaaact gagcatagct cagtttttat
    12721 tttacacctt atttttgtac agcattacta acgcttttag caactacatc ggccacatca
    12781 ttttgaaaag catttggaat gatgtgagtt gtgcttaatt cttcctccgg tattaaacta
    12841 gctatgcctc tcgctgccgc aatttgcatt tcaacagtta tcgtttttgc tctagcatct
    12901 aatgctcctc tgaaaatacc cggaaatgct aaaacattat taatttgatt tggaaaatca
    12961 ctacgaccag ttccaactat gtaagctccg gcttttaagg cttgatctgg ataaatttct
    13021 ggaataggat tcgccattgc aaatacaata ggtttatcag ccatcttgga aatccattct
    13081 gcatgtaatg cttccggagc agaaacacct ataaaaacat cggcattctc caaagcatct
    13141 tctaaggatc cagattggaa ttctctatta gtcaattttg caatcgcttt gtgatgaggg
    13201 gctaatgact cccgatcttt atcgttaata atgccaaatc gatcaactac cgttacatgt
    13261 tttgctcctg ctgatagaag cttccttgtt atagataagc ccgctgaacc tccaccatta
    13321 acaactactt tgatatcttc aatgtctttt ttaactaact tcaaactgtt aaacaaagcc
    13381 gctaatacta cgattgctgt accatgttgg tcatcatgga aaactggtat atcacattct
    13441 tcaattaggc gttgttcaat ttcgaaacaa cgtggtgctg aaatatcctc taaattaatc
    13501 cctccaaaag tcggcgaaat cgcttttact atagaaataa tttcctctgt atcatttgtt
    13561 ttcaacacaa ttggcacagc atctacattt gcaaaacgtt taaagagtgc tgctttacct
    13621 tccatgactg gcattgctgc ctcaggacca atatctccta atccaagaac agctgaacca
    13681 tcacttacta cggctactgt atttttttta gtcgtcaact catatgcaag ctccacatcc
    13741 tctgcaatag ccgatgatac tgacgccacc cctggggtat aggcaatact aagatccctc
    13801 ttatcttcca ccttaacttt tgaaattact tctaattttc caccattttc tctagcttgg
    13861 ttgatagcta attgacctaa attttcactc attttgataa ctccttttga taaatattag
    13921 aataacatgc gtaaaatggc tgtcatcgtg ataacagtaa tagctcctcc taaacgcgta
    13981 gcaacttgag caaatggcat caagttcatt cggtctgctg tacttaaaat ggcaacatct
    14041 cctgttcctc ccataccact ttgacaagct gaaataatac ctgcttcaac aggattcata
    14101 ttaaggaatc gcgatacaaa gaatccaaca gaaataactg taaatacaac actaataaca
    14161 actatgaaat attgtatgct aagcgttgcc acaacatctt ttaacgggat ataaagcaat
    14221 cctagccctg ccattagagg aaatgtaaaa ttaccagaaa taaatttata aagttgctta
    14281 gctccatttt gtgtttctct aggaataaca ttgatatatt tcaaaatagc tgctgcgata
    14341 atcattaata cgggaccagg aaatccagtt aattgctgca acaaatgtcc aaaaataaag
    14401 agactacatg ctgttaaaac acctccaccc attttcttaa cgtcaattgg gccagaatga
    14461 tcagcaataa tatctgacat gtcctctcct ttatttaacc ttactaattg cccttgacca
    14521 gacaagtgtg gtttcttttc tcccaaacga ttcaatagtg cagtacataa aatggcaaag
    14581 aaattaccaa taatagtggc tgggatgagt tgagcaacta gttgttcgct agctacaccg
    14641 gtaattgaac tatagcctaa cgataatggt aaaatacctt caccaatacc accagctaaa
    14701 acgggtgtta cgatataaaa caaagtgtgt cgccactcta agcctaaaat aacaccgaca
    14761 aatgtcccta ccatcatcgc acaaaccatt cctaataaca tggggaaaat cattcttagt
    14821 aatccctgaa tcaacatttt ccgattcatc cctaaaatac taccggacac taaacaagca
    14881 atataaaaat aaagaaagtt agcttgcttc atcaacatat ttgttgactc taaaacattt
    14941 ttattaatga ggttgaaaaa caccaaaata gatggtacta ataaagataa aatagctggg
    15001 ccaccgaagt ttttaaaacc agggatgcta gctccaatag tacctaagaa ccatcccatt
    15061 gttaagatga ctgcaaatcc tcctagcata ttgactggca atttttgtaa aaagcctgct
    15121 agaagaatca ataatgctaa acaaacatat actggtaagg gaactgagcc taccttaata
    15181 tgaaatagtt tagtctttaa gtcttgcttt tgacctctag aaccctcatt attcactacc
    15241 ttcacgtctg ccatgaaagc acctcctttt acttcttatg tacagtttac tttgggactt
    15301 agtgataagg aagtttattt aagtaattga attatttcaa cttatttaat taatttaagc
    15361 tggattctag tttttaggct atgttatact aaactcggag gatgctgatg aaaaaaaaac
    15421 tatctctttg ggcatttcta tcattgattt tagtcactat gaccatttgt atattttcta
    15481 ttttttatta tgtcactata catcagtcct atcgtatggt aagggtacag gaagaaaaaa
    15541 tcttaaaaaa tactggttat gctttatcga gaaatccaca agttatacaa acactcaaag
    15601 ataatcacta taatcaaagt ctccaaaaac aaatgctatt ccttagcaaa aaatcaaatt
    15661 tagactatat tgtccttata aatttaaaag ggatccgttt tacccaccct gactccacta
    15721 agattggtaa accatttcaa ggcggtgatg aacaagctgt ttttaaggga aaagctatta
    15781 tgtctactgc agaaggttct ttaggaaaat ctttacgata cttaattcca gtatatgatc
    15841 accaaaaaca ggttggggca attgcagttg gcttaaaatt aacgacactt ggagacttat
    15901 ctcaaagcag cataaaagag tttagtaagc ctctcttgat tagtattttg attagcttag
    15961 tcgtcacaag tataatttct tatggcttaa aaaaacaact tcacaacctc catccaagtg
    16021 atatttttca acatttagaa gagcgcaatg caacactgga ccaaattcaa gccgctgtat
    16081 ttgttattga ccagaggcat atcatcaaaa gaaataatcc tgcagctagt ttattattca
    16141 aaaaagaggg gcaacgagac ctattttcag gtaaattatt agaatcttta attcctcaac
    16201 tcaagcagga tcatttttca aaaaaaactg aacaagtatt acattttcaa ggacaagact
    16261 atttactatc tatctcgcca attacggtta aaacacaaaa tagaggctat gttgtcttct
    16321 taaggaatgt tactgaaaca cttttcactt tggatcaatt agcacacact acagcttatg
    16381 cttctgctct acaggcccaa acacatcaat ttatgaatca acttcatgtt atttatggac
    16441 ttgcagacat cgaatactac gacgagttga aaatttattt aaaagaatta ttggagccac
    16501 aaaatgaatt tttagcacgc ttatcaatgt tagttagaga gcctcgactg gctagtttca
    16561 ttattggcga aagagaaaaa ttcgcagaaa aacacatcaa tctatccaca gaaattttag
    16621 tagaaatccc taccaagtca actgttgagg atgttaataa ctatctcttg ctccacagat
    16681 atattaatac gaagatactc acgctattaa attcaacgac tttagtttct ttacgtttaa
    16741 attatcaaaa taacctcatt gaaacagatt atcaatggga gaatgagaag tggttactca
    16801 atgactacca tcagtatttc aacgacgctt attttcaaca gttacttgta gattcaagag
    16861 ctacttataa gatctcttta aaacaaaata aggttattat aagaacaaat attatctact
    16921 cagaagggtc ttagtatgga cgtattaatt atcgaagatg atcctatggt ggaatttatc
    16981 caccgcaatt accttgaaaa actcaattat tttcaaaaca tctattcaac tgctagtcaa
    17041 acacaagcta ttgcttatct taatgacatt aaaatccaac ttgttctttt agatattcat
    17101 attaaagaag gcaatggtct agaattatta aagttattac gcaatcaaca ccaaaatacc
    17161 gaagtcattg ttatttcagc agccaacgag gcgcacactg tcaaagaagc atttcattta
    17221 ggtattgtag attacctgat aaaaccattt acatttgaac gctttgagtc tagtattgaa
    17281 aaattcctaa atcattatca tacatttgaa gctgataaaa tctatcaaga caacatcgat
    17341 cactttcaaa aaatagattc tggatggcta gagggagagg ttaaacttga tgagaaaggg
    17401 ctctctgaaa ttacctatca gcatattctt gatgccattc aagagttaga gcagcctttc
    17461 actatccaag agttagcaaa atgcagtcaa ttttctcatg tctctgttag gaaatatatt
    17521 gcctatatgg aagaaaaagg ccttctgaca agtcagcaaa tttatactaa ggtcggtcgt
    17581 ccttacaaag tttataagct tataaaaaaa acttaggcct gtagacctaa gtttttttaa
    17641 tcttcgtagc catttggatg actagaatgc catgcccatg ccgatgaaat aattttatca
    17701 atattatcaa atttaggctc ccacccaagc atttgacgag ctttctctga ggaagcaata
    17761 agcgtatctg gatctcctgg acgacgagct gccttttgag caggaatttc tttcccagta
    17821 atacgacgag ccgcctctaa catctgaagg ttagaaaaac ctgtcgaaga tccgagatta
    17881 aacacgtttg attcattccc ttggcgaagg taatcaactg ctaatatatg agcatctgcc
    17941 aaatcaaatg gatgcacata atctcgaaca ttagttccat ctggagtatt gtaatcatct
    18001 ccaaaaatca ttattttgtc acgtactcct tgagcaactt gaagaatgat tggtaacaaa
    18061 tgtgtttctg gtttatgatc ttccccaatt gagccatccg gtttatctcc agcaacgttg
    18121 aagtaacgta gagcgacaaa cttaataccg taggcttgat cagcccattt cataattgtt
    18181 tccatcataa gcttactttc gccataagga ttaataggat tttgaggagc tgtttctaaa
    18241 ataggaactt gttctggaat accatatgtt gcagctgttg atgaaaagac aatatttttg
    18301 atatcacatt cattcattac ttctaaaagt tttatcattc cagcagtgtt attatcaaaa
    18361 tattttaaag gattttccat tgactcagca actagtgaaa atgccgcaaa atggataaca
    18421 gcatctacat caggattttc cctaaaaact tgacgcataa acgttttatc ggataaatct
    18481 ccttcataaa aaatcgcatc tgaatgcacc gcttgacgat gacctgttac caagttatca
    18541 acaacaataa ctttctcttt tccttgtgtg attaactgat caaccatatg cgagccaatg
    18601 taaccggcgc cacctaaaat cagaactgcc atatttttct cctatttcaa tactctttta
    18661 acatcatttg ttacctcatt atactgagaa taagcttttt ttgcaaatat ctaccgaaat
    18721 tagaatagct ctatgcagaa ggcatcccac ggcttgagag tgttacttga tagtacttct
    18781 tgaacctctg tatttgaaat aatagtttct ttaacagctt ctggaaattc aaaagattgg
    18841 ttttgatcgg ataagtttgc aactattaaa taacgttctt tatctgtttg acgaacataa
    18901 gcaaatacct tatctgctgt ttccaaaagt ttaaaatcag catcaactaa ccagtcgtgt
    18961 tctttacgta aagcaactaa tttttgataa gtataaaaaa tactttccgt atcatttaac
    19021 gctgcctcca cattaatttc ctgataattg ggattgactg ctagccatgg ggtaccgtta
    19081 gtaaaaccag cttgatgtcc ttgactccac tgcattggag tacgagcatt atcacgaccg
    19141 actttacgaa tactatctaa aattgttgct tcagataccc cattatccat ggcttcttta
    19201 gcatagttaa gggattcaat atcatctaca tctgctaaac actcaaatgg ataattggtc
    19261 ataccaattt cttcaccttg atagatgtaa ggcgttccac gcatcaaatg tagtaggatt
    19321 gctagagcct tggcagattg ttttctgtta tcactatcat ttccccaaat cgagagcacc
    19381 cgaggaaggt cgtgattatt ccagaataaa gaattccaac cttggccaag ctccaattgt
    19441 gtttgccatt ttgtaaaaat atctttcaat gctggaacat ctaatccgtc actgtaatcc
    19501 cacttaggag cgtcaggttt atgttgaaga cctacatgtt caaattgaaa gaccatagat
    19561 aattcttcat tatcgggatt tgagtattgt tttgcaatgt ctggcgttgc tccccaagtt
    19621 tcacccaccg tcaataagtc atgctttcca aaactagctc tgttcatttc tttaagataa
    19681 tcatggagtt ttggtccatt accagtaatg cccttatctg gaattttccc aattaaatca
    19741 atgacatcca tacggaaacc accaataccc ttatcaatcc agaaattcat catatcataa
    19801 attttatgac gtaaagcttc attctcccag ttgagatctg gctgacgttt gctaaaaagg
    19861 tgcaaataat attgacctga tgctttatca tattcccaag ctgagccact aaaaatagaa
    19921 gtcaaatcgt taggttcatc tcgccaaata taaaaatcac gttcaggact attagggttt
    19981 tctcgcgctt ctacgaacca agcatgttca tcagaggtat gattcactac caaatccatg
    20041 atgattttaa tcccgcgttg atttgcctct tgtaacagtt ggtccatatc attcatgtca
    20101 ccgaaaatat ctgcaatggc ttgataatct gaaatatcat aaccattgtc atccattgga
    20161 ctttggtaaa caggtgataa ccagatagca gtaatcccta atttttcaag gtagtctagt
    20221 ttgctagtaa tccctttaat gtcccccaca ccatcaccat ctgaatccat aaatgaacgt
    20281 gggtaaattt gataaatcgt agctttatgc caccagtgtt tcttcattat tcttctccta
    20341 tcaataattc attcatcttt tattttaacg tttttcctct tttagtgcaa gcgtttgcgc
    20401 aaataacatt ataaaaaggc tggaacccaa cctttttatg aatatcgctt ataccttaac
    20461 gaatagcttg ttcagtatct gcatcaaaga aatgcccctt agcaacatta aacgttaggt
    20521 taactttttc tcctggatta tggaagtcac gcgcttcaac acgagaagca aattctgttt
    20581 gacctaactt aacataaagc atagtttcgc taccgagtaa ttcagaaact aaaacttctg
    20641 cttcaacatt ggcatttgga taagtatctt ggacaagaag attgcttgaa atgtcttctg
    20701 gacgaatacc aaaaatcaat tcttttcctt tatagcctcg tgattctaaa agtttttctt
    20761 gaccttcagg gatagcgatt cgaagaccat cttcactaat aatcatacca ttttcaactt
    20821 taactttgaa gaagttcata gatggactac cgatgaatcc cgcaacaaat ttattagcag
    20881 gaagattata gagttcttgt ggtgaaccaa cttgctcaat tttaccaatt gtaccatcac
    20941 catcagggtt cttagtagct gacataatga caatacggtc tgccaaagtc atcgcctctg
    21001 tttggtcgtg ggtaacataa atagttgttg aaccaatacg ttgatgaatt tttgcaattt
    21061 cagcacgcat tgaaacacgt aatttagcat ctaagttaga taaaggctca tccattaaga
    21121 acactttggc atcacgcaca atagcacgtc ccatagcaac acgttggcgt tgtccacctg
    21181 acaaatccgc aggtttgcgc tctaagaatt cagtaaggcc aatattagct gctgcttcac
    21241 gaacgcgctt atcaatttct tgttttgaga atttacgcaa tttgagccca aaagccatat
    21301 tatcgtaaac agtcatatgt ggataaagag catagttttg gaaaaccata gcaatatcac
    21361 gatctttagg tgatttgtca ttgacaactt cgccatcaat cttgagctca ccttcactaa
    21421 tatcttcaag acctgcgatc atacgaagtg ttgttgattt cccacaacct gatggtccaa
    21481 caaaaacaat aaattctttg tctttaatat ccaagtcaaa gtcttcaaca gaataatgcg
    21541 atgcacttgg atattttttg taaatatgat ttaaatttaa ttctaccatt agattgacct
    21601 cttttattat tgtgatttta ttatagcaaa gaaagggttt tcatataatg gtaaccttgc
    21661 ctaaatttct tgtgcaaact aactaaattt taggtatcca tcgaaattaa ataggcatag
    21721 gctaaatcat ctaatatttt taaattaagc cccgaagatt gggtaaattt atctaattta
    21781 tattgcaatg aattccgatg aatgaaaagt gccttagccg ttttagcaag gttcccttgc
    21841 tcttgccaca aggcttttat tatagccgaa gtatcactcg tatcaaggat atgttgtaaa
    21901 cattttgctt taatagaagg catgggcgat tgatttgcca aagcccataa agccatcttt
    21961 ggaaatcgac agtaatgttg ttcggccttg tgactaagaa aaagcgtaaa aagtttatat
    22021 tcttcttcat ataattcttt ccagtccact gcttgtagat gcgtccaact atttccaaaa
    22081 tagcatctta aacgcgtgtt aaagtcattt tcaatcgttg gtaaaatatc tgttagtaat
    22141 tccgtagcat taaagaaatt atcctgattt aaaatcatga cactttgggt ttgattaatt
    22201 gtcataattg tttctacgtt tattactaaa ggcgacaaga aatccttaag ttcttctgat
    22261 aaggtaaatt gatgctcaat aaaaataaat tggactgccg aatgagattg gggcaacctt
    22321 ccacgacctt caactagata gcggtaccaa ggattctgag aatccactga actcgcctct
    22381 tccaggcttt gtgtcatttg taataagcat agttcacgct tagttagcga agattttgga
    22441 aagtggagga catgaccatc ttgctcaacg atgatataat cctcactctg tttaacttcc
    22501 tgtttagtaa taacttctgg aaacagttcc ttaaaatcca tggcatcctc cactacagtt
    22561 ttgttcatta tatcatattt ataacctctt tttgagcaca gaaaaagagg agaatgtctc
    22621 ctcagaaagt ttctgttata aaatagtgat ccgaatagaa tgctgtttcg actgctttgg
    22681 attaagccgt acgatatttt gtttatcttc taaaatatca gactcttcaa tagaagttga
    22741 caaggaactc cagggttcca aagccaaaaa aggaccacca ttgttacttg tccaaagaat
    22801 aagattctca aaatcatcaa agtcaagttg gattcctttt gcatgatttc tagattttaa
    22861 gtagaccgtc ttagatcgta gttggtctaa agtaatagca tctttttcaa ataaagcata
    22921 attaagagat aattgttttt gattttctaa aaaaggatgt ctagcttgta aatctaacaa
    22981 gcctgtatcc ggaaacgact caggaattgt gcaagtttca aactgttcaa actctagata
    23041 gtaatcacta aatacttcct tttcaaacaa agggcaatta aagcctgggt gagctccgat
    23101 agcatatggc atattttttt ctgattctaa gtttttgaca atatatgtaa tggcaatgct
    23161 tttatttctt aaggtgtaag ttatttctaa actaaaacga taaggataat ttatcaacgt
    23221 ttcttcacta cttttaattg taaaacgaac agaagaatcc gatatgtaat catagtcgaa
    23281 tgttttgtga cgtacaaagc catgccttgg tatgataccc gtaaaatgtg gagcttgagc
    23341 tggtctgtaa atgacgcaat catttcgaac acttccgcat ataggaaata aaataggagc
    23401 ttgcccaccc caataagtgg gttccccttg ccaaaggtac tcaacgcctt gactatctgt
    23461 aatagaagtc attgttgcac ctaacgcttt taatgtcact tgcaactcat gattttgtat
    23521 cgtaatagcc attatttttc ctttcataaa gagcaataat tgctttacca acttttctct
    23581 agcaattatc tttccttcca agaagttgct gttttttgca aaattttatt tagttcatca
    23641 atattttgaa aacctattgt acgtaaccat tgacgtgcac cagcttctcc tttttcaaca
    23701 tactctttaa cacttccagc ccaagttgct ctaccacaaa ggacaccgtt aaatttagca
    23761 ccagcttctt tagcaaacac aagagtctct tgaaaaagct gagcaggaac tcctgcactc
    23821 aaaaaaatgt aggggagtag tgtcgcctga tcttgttctc taaaataagc cgcagctgtc
    23881 gctttattat aagcagtctc tccttgcgca aaaccttcta catagtccat attgacaggc
    23941 acttccacct tcaagacatc aatattaaat cgaggatttg aaaaaacttt catagcttca
    24001 ataaccttac gtggcttaat tttggcatac tcaatacctg agctatccgt aatcttttca
    24061 tcatatgaca aaatttcaag gaaaaaagga atgtcttctg caacacattc agaaccaatc
    24121 cgttcaatat aagcttcttt ttcttcgttt acttcatccg aactatcaac atcataataa
    24181 agtaagaact taactgcgtc tgccccctct tctttaatgc gtttcgcaga ccaaatgtct
    24241 aaacagtctg ggagtcgctt ggtactactg gtatcataac ctgttttttc gtacgcaagt
    24301 aaaagaccgg cattgtcatc taaaactttc gtagctggca acccatattc tggatctaac
    24361 aacattgatg aagcgtaagg ggttaattct tcagctacca aaactttaag tgcttctatt
    24421 tgagaaacag tgggctcctc agattgatat tttgccatta atcgcttcaa tgctccacgt
    24481 tggtcaaaag ccaaggccga aataatacca tttttatcac tcaattgttc catatgtttt
    24541 tgtttttgtt ccgttaatcc catcctatac ctctttcact tggatttgac tgattaattc
    24601 ttgatagtag gtcagattga tatgccctgt taatgtttct tgtgcattaa gcatacccaa
    24661 gacattagct ctcttaagta aatttatatc atctagttga tgttctaaag cagacgcgat
    24721 tcctgcaacc gttgcatccc ctgaaccaac tggattgata acttctattt ttggaatcgt
    24781 cacttgatag tagtgattcc catgtttagc aaaagctccc ctagcaccta aggaaacaat
    24841 aacccaatca attcctctaa aaatatcttg ctgtaaggta gattttaatt cgtctaaact
    24901 atagctaatt ggctttccaa ttaattgtgt taattcttca aggttaggtt taatgacggt
    24961 cggcttagca ctagatttta aaaccgaacg taatgcttct cctgaacagt ctaaaacaac
    25021 tttcttatta aagtgattcg ctagggagat taacctagca taataatctg aggggagtcc
    25081 ctttggtaaa cttcctgata ctgttatgat agttgcagca tcaaagataa gttttagatg
    25141 attcaaaaag gcgcctgctt catctttagt aatatgtgga cctttttcta gaatttcagt
    25201 ctgctgaccc tcatgtaaaa ctgcaataca atgacgtgtt tctccaccaa tacttaaaaa
    25261 ttggtgtctg atttccaatg catctaagcc acttcggatg aaatctccaa aatgcccccc
    25321 taatagacca gttgccacta cattatcgcc aagttgagaa agaacacgag tcacatttaa
    25381 tcccttacca ccaggtgttt tggagacgtc ggtaacccga tt
//
LOCUS       DATRSG010000024        24277 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRSG010000024 DATRSG010000000
VERSION     DATRSG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24277
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.024"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(34..744)
                     /locus_tag="VBD89_000967"
     CDS             complement(34..744)
                     /locus_tag="VBD89_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7135510.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(741..1439)
                     /locus_tag="VBD89_000968"
     CDS             complement(741..1439)
                     /locus_tag="VBD89_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7135511.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            1607..2371
                     /locus_tag="VBD89_000969"
     CDS             1607..2371
                     /locus_tag="VBD89_000969"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO7135512.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSLEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            2479..4986
                     /locus_tag="VBD89_000970"
     CDS             2479..4986
                     /locus_tag="VBD89_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO7135513.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFN
                     DQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSH
                     AAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            5072..6265
                     /locus_tag="VBD89_000971"
     CDS             5072..6265
                     /locus_tag="VBD89_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7135514.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLGTLKEAVRRLHVFMG
                     SNEIN"
     gene            6286..7632
                     /gene="asnS"
                     /locus_tag="VBD89_000972"
     CDS             6286..7632
                     /gene="asnS"
                     /locus_tag="VBD89_000972"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO7135515.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(7672..8229)
                     /locus_tag="VBD89_000973"
     CDS             complement(7672..8229)
                     /locus_tag="VBD89_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7135516.1"
                     /translation="MTKSPARLISFISIAIAINLIGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYIDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(8226..9209)
                     /locus_tag="VBD89_000974"
     CDS             complement(8226..9209)
                     /locus_tag="VBD89_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO7135517.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRRDISVYVGNNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFVSMGGNFKSHGN
                     CSPVAEYNYWYDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSTF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(9353..9397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(9455..9868)
                     /locus_tag="VBD89_000975"
     CDS             complement(9455..9868)
                     /locus_tag="VBD89_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO7135518.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVNCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            10022..10912
                     /gene="rapZ"
                     /locus_tag="VBD89_000976"
     CDS             10022..10912
                     /gene="rapZ"
                     /locus_tag="VBD89_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO7135519.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQKEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            10909..11883
                     /locus_tag="VBD89_000977"
     CDS             10909..11883
                     /locus_tag="VBD89_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO7135520.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            11880..12791
                     /gene="whiA"
                     /locus_tag="VBD89_000978"
     CDS             11880..12791
                     /gene="whiA"
                     /locus_tag="VBD89_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO7135521.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            12806..14203
                     /locus_tag="VBD89_000979"
     CDS             12806..14203
                     /locus_tag="VBD89_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO7135522.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            14345..15865
                     /locus_tag="VBD89_000980"
     CDS             14345..15865
                     /locus_tag="VBD89_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO7135523.1"
                     /translation="MRKKFLLLISFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(15978..16238)
                     /locus_tag="VBD89_000981"
     CDS             complement(15978..16238)
                     /locus_tag="VBD89_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO7135524.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(16347..17282)
                     /locus_tag="VBD89_000982"
     CDS             complement(16347..17282)
                     /locus_tag="VBD89_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO7135525.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLEIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGIPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
     gene            17548..18570
                     /locus_tag="VBD89_000983"
     CDS             17548..18570
                     /locus_tag="VBD89_000983"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO7135526.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPNSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVFAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKEIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRALIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            18629..19072
                     /locus_tag="VBD89_000984"
     CDS             18629..19072
                     /locus_tag="VBD89_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO7135527.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            19149..19424
                     /locus_tag="VBD89_000985"
     CDS             19149..19424
                     /locus_tag="VBD89_000985"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO7135528.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            19417..20613
                     /locus_tag="VBD89_000986"
     CDS             19417..20613
                     /locus_tag="VBD89_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO7135529.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            21197..21544
                     /gene="rplS"
                     /locus_tag="VBD89_000987"
     CDS             21197..21544
                     /gene="rplS"
                     /locus_tag="VBD89_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO7135530.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            21655..21726
                     /locus_tag="VBD89_000988"
     tRNA            21655..21726
                     /locus_tag="VBD89_000988"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21688..21690,aa:Arg,seq:cct)
     gene            complement(21689..22303)
                     /locus_tag="VBD89_000989"
     CDS             complement(21689..22303)
                     /locus_tag="VBD89_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7135531.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
     gene            complement(22306..22572)
                     /locus_tag="VBD89_000990"
     CDS             complement(22306..22572)
                     /locus_tag="VBD89_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO7135532.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            22572..22976
                     /locus_tag="VBD89_000991"
     CDS             22572..22976
                     /locus_tag="VBD89_000991"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7135533.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            23138..23338
                     /locus_tag="VBD89_000992"
     CDS             23138..23338
                     /locus_tag="VBD89_000992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7135534.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            23363..23620
                     /locus_tag="VBD89_000993"
     CDS             23363..23620
                     /locus_tag="VBD89_000993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7135535.1"
                     /translation="MNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKWC
                     TDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            23746..23955
                     /locus_tag="VBD89_000994"
     CDS             23746..23955
                     /locus_tag="VBD89_000994"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7135536.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(24128..>24277)
                     /locus_tag="VBD89_000995"
     CDS             complement(24128..>24277)
                     /locus_tag="VBD89_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEO7135537.1"
                     /translation="GAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQTDEY
                     GNIIV"
BASE COUNT         7818 a         3862 c         4836 g         7761 t
ORIGIN      
        1 tttcatctct ttttattaat tgtagatatc atgctagtgg ataaaatttg aaataaacgt
       61 agccaattgt ttttcagatg atttgtgaga aagggtagct ttaccatccc ctttttgatg
      121 tccgtagtta ccaaatcggc tatggtttcc cccgacaata gttcgaaaag tacttgaatt
      181 tgggaggcgt tttttagctt cctcatattt ttcccagttt aaaatatgat cgttacttgc
      241 tgtaatggag agtactctta actttttatg agataggtcg gatttacttg aagggtagga
      301 agctaataat atgagccctc taacaggggc tactttggca ttttggctag caacaactcc
      361 tcctaaagaa tgtccagcaa ggtaaacatt agttaagtgg ttctgattaa taattgtttt
      421 agctttataa ggtgataaaa ctggcaaatt taaaggtgtc ttcaaaatat aggtattata
      481 ccctttatca gctaaatctc tagcaagttg agaataagct tcttcttcca caagagcccc
      541 ttgatagaaa ataatatttg cttttacctt acccttgctt ctaaaaagaa ggtagttctt
      601 atggtttgta gcggttttag caattctcct agctaatggg gaggcttggt aagtattgaa
      661 gtaaatggtc atcccgacgg ccattaaaat agcaaggata cttgttgtta accaaagaaa
      721 aatagagtga cgttttatgt tcattggaag aaaggattaa agtttttttc gtggccaata
      781 gttgtattca tgccatggcc tgggtgaaca ctataatgac taggtaaggt gaatagttct
      841 tggcgaatac cagtaatgag gtcctcaaag ttactggtag gtaagtcagt acgaccgata
      901 gtttcacgaa atagagcatc gccagtaacg actaattcat ctgagtggaa aacaaatgag
      961 actcctcccc aagaatgacc aggtgttggt aaaactgtga attcaaaacc atttagttga
     1021 tagggttgct ttaatttaaa aaagttctct gccggtcttg ctataacatt aatgatatca
     1081 tcatgacggc ctaacccaga taaattatca tctggggaag atagccaagc ggcttctttt
     1141 tctgaaacat aaacaggtgg attatcaaaa gtatctctga ccaaatctaa actaaaaata
     1201 tgatcatagt gagtatgagt taagaggata gccactaaag gtttttcaaa ggatttaatt
     1261 ttagcaatga tttcttggcc attgctccct ggatcaatga ggataacagc ttgatcatta
     1321 acgaggtaat aagtattttc accagcaatg tggtttattg ttttatagat tttcataatg
     1381 atagtgtacc aaaaaatcaa gactttgtta aaaaaagagt ggcggaaaat aaatggcaac
     1441 tagtttcaga aaatatggga tctcacagca atttcttgac accgttttca taaaggtgta
     1501 tcataaaagt aatcaataag ccaagcctaa agctgaactt agtttaagaa cactgacttt
     1561 ttgagagcaa cctaattggt tgactaaaga atatgaggtg gaagatatgt caaaagttgc
     1621 tattgttact ggtgctggtc aaggaattgg atttgctatt gcgaaacgtc ttcatgcaga
     1681 cggctttaaa ataggcgttt tagactataa tgaagagact gctcaggctg cggttgataa
     1741 actatcactt gaggatgctg ttgcagttgt agcagatgtt tcaaaacgtg atcaagtctt
     1801 tgatgctttc caaaaagttg tagatacttt tggtgatttg aatgttgttg ttaacaatgc
     1861 aggtattgcc ccaacaaccc ctcttgacac tattactgaa gagcagtttg aaaaggcttt
     1921 tgcaatcaat gtcggaggaa ctatttgggg aagtcaagca gctcaaaaac attttagaga
     1981 attaggtcat ggcggaaaaa ttataaatgc aacttcacaa gcaggatgtg aagggaatcc
     2041 taatttgact gtttatggtg gtacaaaatt tgcagttcgt ggtattacac agactttggc
     2101 aaaggatttg gcatcagaag gaatcacagt caatgcctac gcaccaggta ttgttaaaac
     2161 acctatgatg tttgatattg cccatgaagt tggtaagaat gcaggcaaag atgatgaatg
     2221 gggtatggag cagtttgcaa aagatatcac tttgaaacgc ttgtctgaac cagaagatgt
     2281 tgctaatgca gtaggcttct tagcgggaga tgattcaaat tatatcactg gacaaacaat
     2341 agttgtcgat ggtggtatgg tattccatta aaaatgatta atgctaacac tgtgagtgaa
     2401 actcacagtg ttttttatat ttcttaaaaa attatcttat cttggttatt ggagtgatgg
     2461 catctttgag gtacagtcat gttttgtttt atagatatag cgtgctataa tagacttact
     2521 atgacccaga aaaaacttag aaaatatgca gtagttgact tggaggctac tggagcaggt
     2581 cccaatgctt ctattattca ggttgggatt gttattatcc aaggaaataa aattattgat
     2641 agttatgaaa cagatgttaa tcctcatgaa tctttggatg agcatattgt tcatttgaca
     2701 ggtattacgg ataagcagtt ggttaaagct ccggattttg gtcaagtggc gcatcacatt
     2761 tatcaattga ttgaagattg tatttttgtt gcccacaatg taaagttcga tgcaaattta
     2821 ttggctgagc aactattttt agaaggcttc gagttacgta caccaaggat agatacagta
     2881 gagttatcac aagtgtttta tccctgttta gaaaaatatt ctttaggggc attagcagaa
     2941 agcttaaata ttgaattaac agatgcgcac actgctattg cagatgcaag agctacagca
     3001 cagttattca ttaagttaaa agctaaaatt agtagtttac ctaaagaagt gttggagacc
     3061 attttaacat ttgctgataa tcttttgttc gaatcctatt tattgattga agaagcttat
     3121 caagaagctg atttcgttaa tcctaaagaa tattatttct ggcaaggatt ggtcttaaaa
     3181 aaagaaaaag ctgttggaaa gcctaagaaa ttatctagtg attttcaagt taacatggct
     3241 ttgttaggta tggatgctcg ccctaagcaa attgtttttg ctgacttggt taaagcgcat
     3301 tttaatgatc aaacaacaac gtttttagaa gctcaatcag gacttggtaa aacatatgga
     3361 tatctcttgc ctttattgga tcagtctcaa aaacaacaaa taatagtatc tgttccaaca
     3421 aagattttgc aagatcaaat aatggctaaa gaaataaagc acattcaaga actttttcat
     3481 attccctgtc acagtatcaa aggaccgaga aattacttga agttagatgc gttttataag
     3541 agcctccaag tgcaggatcg aaatcgattg ataaatcgtt ttaaaatgca actgctagtg
     3601 tggttgacgg aaacgactac aggtgatttg gatgaaatta aacaaaaaca acgattagaa
     3661 tcttactttg accagctaaa acacgatgga gaagtgactc aatcatcact tttttatgat
     3721 ctggattttt ggaaacgcag ttatgataaa gttgcacaaa gtcaacttgt tattattaat
     3781 catgcttatt ttcttgagcg tgttcaggat gacaaagatt ttgcaaaggg gaaggtttta
     3841 gtttttgatg aagctcagaa acttgttttg ggactggaaa atttttctcg tgggcaactt
     3901 gatattagtc accaattgca ggttattcaa aaaataattg atagtcctat tccgttatta
     3961 caaaagcgcc ttctggaaag tatttcttat gagttaagtc atgcagcaga acttttttat
     4021 cgtcataatt cttttgaatt ttcagagacc tggttgaaac gtctaaaaaa tagtataaac
     4081 gctttagaag tagtaggatt agatgattta caaacttttt tcacagctac ttatactaac
     4141 tattggtttg aaactgataa agtcaatgag aagcgtttaa ccattttacg tggggcgaga
     4201 gaagattttc ttaaattttc agagttcctc cctcccacaa aaaagaccta tatgatatca
     4261 gctactttac aaatcagtcc caaagtctac ttgtcagatc ttttaggagg attttcaagt
     4321 ataagcacag agaagatagc gcatgagaaa aatgccaatc agaaagtatg gattgatacc
     4381 tctatgccta atatacttga tttatcaccg gaacagtatg cttacgaaat agccaagcgt
     4441 ttacaagata taatgacgtt aaagcagcct accttagtct tattaacttc taaacagaca
     4501 atgtttatgg tatctgatta tttggataag tgggagataa aacatcttac tcaagataaa
     4561 aatggcttag cttataacgt taaaaaacgt tttgatcgtg gagaaagcaa tcttctttta
     4621 ggtacaggat ctttttggga aggtgttgac tttgtacatc gtgatcgtct tattgaagtt
     4681 atcacgcgat tgccttttga taatcccaaa gactatttta tccagaaact atcgcagagt
     4741 ttaacaaaag aggggaaaaa cttcttttat gactattctt taccaatgac tgtcttaaaa
     4801 ttaaaacagg ccttgggacg tactacaagg agagaagaac aaaagtcagc ggtaattatt
     4861 ttggatagca gattggttat taaatcttat ggacaaacca tcatgcattc tcttggtaga
     4921 gattttgaaa tttctaagga aaaaattaac aaggtcttga ctgaaatggc taaatttttg
     4981 atataatggt aaagatcatt gtctaaaaat aagatgttga cagtgcgact caagattaaa
     5041 taatagtagt acgttgataa aaggaggagt gatgacctat ttgtcagaac gtgtactcaa
     5101 tatggaagag agtgttactt tagctgcggg agctaaagca agagaactca gagtacaagg
     5161 aagagatatt cttagtttaa ctttagggga gcctgatttc gctactccta aaaacattca
     5221 gcaagcggct attgaggcta taacagatgg acgtgcaagt ttttacacac catcaagtgg
     5281 tttgccggag ttgaagtcag caattaatgc gtattttgaa cgtttttatg gctactccct
     5341 taaacctaat caggtggttg tgggaactgg tgctaagttt atcctttata ctttctttat
     5401 gacagtactg aatcccggtg atgaagtaat tattccgact ccttattggg tatcttatgc
     5461 tgatcagatt aagatggctg aaggtaagcc agtttttgtc acagcaaaag aagttaatca
     5521 cttcaaagta actgtggaac agttagaagc tgttaggact gataagacaa aagtcatact
     5581 tcttaatagt ccatcaaatc ctacaggtat gatttataaa gctgaggagt tggaggctat
     5641 tggaaattgg gcagtcgaac atgatatcct aattttagca gatgatattt acggtcgatt
     5701 ggtttacaat ggcaatattt ttacccctat ttcaagcctt tcagagtcca ttcgaaacca
     5761 aacgatagtc ataaatggag tttctaagac ttatgcgatg acaggttggc gtgttggttt
     5821 tgcagtgggt aatcatgata ttatagcggc aatgtctaaa gtagtcagtc aaacaacttc
     5881 taaccttacg gctgtctcac agtatgctac tattgaggct ttaaatggtt ctcaagagtc
     5941 atttgaaaaa atgaggctag catttgaaga acgccttaat atcatttatc cattgttgtg
     6001 tcaggtccct gggtttgagg ttgttaaacc acaaggtgct ttttatcttt tcccaaatgt
     6061 tacaaaagca atggaaatga aaggatatac ggatgttaca gcattcacgg atgccattct
     6121 tgaagaagtt ggacttgctt tagtaacggg tgctggtttt ggagcgccgg aaaatgttcg
     6181 tctgagttat gctacagatc ttggaaccct taaagaagct gttcgtagac tgcatgtctt
     6241 tatgggaagt aatgaaatta attaaataaa aatagagaga aacttatgtc aaaaaaactt
     6301 atttcaattg ttgatgttaa agattatgtt ggtcaagaag ttactattgg tgcttgggtt
     6361 gctaacaaat caggtaaagg taagattgcg tttgtacagt tgcgcgatgg ttctgccttt
     6421 ttccaaggtg tcgcttttaa accaaacttc attgaaaaat atggtgaaga atcaggtctt
     6481 gaaaaatttg acgttattaa acgtctcaac caagaaacat ctgtttatgt aacagggatt
     6541 gttaaagagg atgaacgttc aaaatttggc tatgagcttg atattactga ccttgaagtc
     6601 attggagagt cacatgaata tcctataact cctaaagagc atgggacaga cttcttgatg
     6661 gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac
     6721 gctattattt attcaactta tgaattcttt gaccaaaatg gttttatcaa atttgatagc
     6781 ccaattttat cagaaaatgc agcagaagat tcaacagagt tattcgaaac agactatttt
     6841 gggaaaccag catttcttag ccaatctggt caattatacc ttgaagctgg agcaatggct
     6901 ctaggccgag ttttcgactt tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc
     6961 catttaacag aattttggat gatggatgcc gagtattctt tcttgtcaca tgaggaatca
     7021 cttgaccttc aagaagctta tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct
     7081 caagcacttg atattttaga acgtgatgtt gaggctctca aacgttatat cgcagagcca
     7141 ttcaagcgtg tttcttatga tgatgctatt acacttcttc aagagcatga agcagatgaa
     7201 gatacagatt atgaacattt agagcatggt gatgactttg gttcaccaca tgaaacttgg
     7261 atttctaatt actttggagt gccgacattt gtagtgaatt acccagcaag ctttaaagca
     7321 ttttatatga aaccagttcc tggaaatcca gaacgcgttc tttgtgctga tcttttagct
     7381 ccagaaggtt atggagagat cattggcggc tcaatgcgtg aagacaacta cgatgctctt
     7441 gtggctaaaa tggatgaact aggtatggat aaatcagaat acgactttta ccttgacctt
     7501 cgtaaatacg gctctgttcc tcatggcggt tttggtatag gtattgaacg tatggtaact
     7561 tttgttgctg gtaccaaaca tattcgcgaa gctattccat tcccacgtat gttacatcgt
     7621 atcaaaccat aataaaaaag cgctagctag tgcgcttttt tattatggtc tttagaatcg
     7681 ttggttaatt aaattgggac tatgtttttt taacaatgtg attgtcgaaa agacaaggat
     7741 aagactgatt agtcgatcaa tataatctgt tccaacttga acaagtatca aactacttgt
     7801 catgtctaat ccaattccat gtaagaattg tgctataata cttgagccac tagaggtaat
     7861 tcctttaaaa aggataactg ttataactga agcaataata gttcctggta aagaaataat
     7921 cagtgatttc cacaataggc tagagggttt acaattcctt ttaatcaaga taccagtaat
     7981 gatagcaact acgatggcga ctggtgaata ataaagtgaa aaaatatcag tagtcatcca
     8041 attgatgagt gcactgagaa aagcagtgct ggcagcgtac catggtccca ggataactgc
     8101 tattagcaat gtacctatag tatctagata gataggtagg cgtaaaaata gagctaaatt
     8161 agccccaata aggttgatgg cgatagcgat tgagataaat gaaattaaac gagcaggtga
     8221 ttttgtcatt tgttaagtcc taacatttct aagtcttcaa tgatgactga attttgtgca
     8281 tgtaatagga cggtcataaa atcattccaa aataaattag tattgactga ggttaaaatc
     8341 ttactgttag catctttttt ataaaaatga tactgatcta caattgtttg tcccatggcg
     8401 attccgtgac aagcaacatc ggtatagcta tcaaaaccag tagcgatatt ttcattaaca
     8461 aagtaagcaa tagctaaagg atcatttatc acacatccaa taatatgctc atattgccag
     8521 tgaaaatcga agtagaactt tgtgattttt tggataaaag ttgatacgtc tggattaata
     8581 cgctccatat aagataaatg attgggggtt aatacaatat gacgtgtgat gtctagtcca
     8641 accatctcta tttttttgtc aaggttttca aatacatatt gtgcagcatg gggatcatac
     8701 caatagttgt attcagcaac tggagaacaa ttgccatgtg atttaaaatt tcctcccatg
     8761 ctaacaaatc gcttgcaatg tttgcctaat ttgggattag tttgtaaggc tcgagcgata
     8821 ttggtcagag gtccgagagc tataatagaa gtatcatttt tgtgttcaaa atagtttgct
     8881 aaaaaacaat ctgctgattc ttcttgaaag ataattggtt gcgccaatgt aaagttattc
     8941 tctccaaggc catccattcc atgagtatct tgagcagata caaattcacg ttgtaaaggc
     9001 aagttattac caacataaac tgaaatgtcg cgacgattta gtaattcaag cgttacaaag
     9061 gtattcttta gtcctagctc aacaggcgaa ttgcctgctg ttatagtaat agcaacaact
     9121 tctaacttag gatgctgaat agcatacatc agtgctaagg tatcatcaat accgggatcg
     9181 caatcgataa taattttttc tttgttcatt tgtctccttt cttttaatat tagtgaatgt
     9241 tctatgcttt tatgaaacta ttaacaatag tcatggcaat aaaaaagatc ttggcagtta
     9301 ccaagatcac acgaaaaaat gaatttagtt ttctaaattc aatcatattt tcagtcactt
     9361 agttttttat tctgggaagt tttcgaacca gtccgagaaa tcctcgatta tgcacgtgtt
     9421 ttattataat atgaaagcac taacttgtaa agattcaaac tgaccattta acagtgtcta
     9481 gattgattgt tttagcattt gaaattaatt ttgaaatcgg acatctgctg tgtgctttgt
     9541 taacgattag cttactatca gataatgaca gtttttcaat actagcaaca gcgttaactt
     9601 ggaaataaaa tcccttacct acttgctttt ccttcatgag ctggcaatta acatcaacac
     9661 gagattttag gtctttgaat cctttttgtt ctaaaatagc ttttatagtt gcatttaaac
     9721 atgttgacca tgctaaagca attaattctt caggattaaa tcctggtaaa tgatttaaag
     9781 ggtgattggt ctccacaatt tttccattgg agagataaac ttttccatca acgccttgag
     9841 tgtttgatga ggttacttca taaagcataa taaactcctt tgggtctagt ttaccaaatt
     9901 tgggagcatt ccgacaagat tttactattt agaatgagtt tcttttcctc tgaagtaaaa
     9961 tcctaattgc tagccatttg tggtataatg gcataaaaca taagagaaag aggtaatcgt
    10021 aatgtcagac gaacaaatca aacttgtgat tgtaacaggt atgagtgggg caggaaagac
    10081 agtggctatt cagtcatttg aagatttagg atattttaca atagataata tgccgcctac
    10141 tctagtgcct aagtttttag aattagcagc acaatcagga gatacaagca agattgctat
    10201 ggtagttgac atgcgtagtc gtcttttctt tagagaaatt aactctattt tggatagcct
    10261 tgaaattaat gataatatta actttaagat tctattttta gatgctactg acaccgagct
    10321 agtgtcacga tataaagaga cacgtcgtag ccaccctctt gctgccgacg gcagagtgtt
    10381 agatggtatt tctctagaga gagaactatt agcaccgctt aaaagtatga gtcagaacgt
    10441 tgtggacact agtgaattaa caccaagaca actacgtaag gtcatttctg aagaattctc
    10501 aaatcaagat agtcaaagtt cttttcgtat tgaagttatg agttttgggt ttaaatatgg
    10561 tatacctttg gatgcggatt tagtattcga tgtgcgtttt ttaccaaatc catactataa
    10621 accagaatta agagataaga caggtttaga tactgaggtt tatgactatg tcatgagttt
    10681 tgatgaatca gacgattttt atgaccatct tcttgcctta ataaagccaa tcttaccagg
    10741 ctatcaaaaa gaagggaaat ctgttttgac tgtggcaatc ggatgtacag gtgggcaaca
    10801 tcgtagcacc gcttttgctc atagattatc agaagattta aaagcagatt ggacagtgaa
    10861 tgaaagccat cgtgataaaa ataagcgtaa ggagacggtg aatcgttcat gagaaaacct
    10921 aaaataaccg ttattggcgg aggaacggga attccagtta ttttgaaaag tcttcgctta
    10981 gaagatgttg agattacagc ggttgtcacg gtggcagatg atggtggttc gtcaggtgag
    11041 ttgcgctcag ttatgcaact aacaccgcca ggagatttaa gaaatgtttt ggttgccttg
    11101 agtgatatgc cgaagtttta cgaacaaatt ttccaatatc gttttgcaga aggtgatggg
    11161 gattttgcag gtcatccttt aggaaatctt attattgctg gtgttgctga aatgcaaggt
    11221 tcaacttata acgctatgca gagtttaaca cagttctttc atacaacagg aaagatatat
    11281 ccatctagtg aacatccgct cactctgcat gctgttttta aagatggcca tgaagtggtt
    11341 ggagagagtc aaatagccga ttataaaggc atgattgacc atgtgtatgt cactaatact
    11401 tataatgaag agactcctac ggccagccgc aaagttgttg atgcaatttt agaaagtgat
    11461 atgattgtgt tgggtccagg gtcacttttc acctctattt tacctaacct agttattcca
    11521 gagattaaac aagctctttt agaaactcgg gcagaggtag cttatgtatg caatattatg
    11581 acacaacgtg gtgaaactga gcattttaca gacgcagacc acgttgaagt attgaaacgt
    11641 catttaggac aagatgctat cgatacagtt ttggttaata ttgaaaaagt gccagagtct
    11701 tatatggaaa acaatcattt tgatgagtat ttggtacagg ttgaacatga tttttcaggg
    11761 cttagaaaac atgccagacg tgtcatctcg tctaactttt taaaattaga aaaaggtgga
    11821 gccttccatc atggtgactt cgttgttgag gaactaatga atttagtagg aacacattta
    11881 tgagttttac agtaaaagtc aaagaagagt tgctaggtca taagagtgaa aataagatgg
    11941 aactgtcagc tattatcaaa atgtcaggta gtttaggatt agctaaccac ggcctcaacc
    12001 tttctattac cacagaaaat gctaaaattg ctcgtcatat ttatagcatg ttagaagaac
    12061 attatcattt gcagccagaa atcaaatacc atcaaaaaac gaacttacga aagaatcgtg
    12121 tgtatactgt ttttattgaa gaaaaagttg atgttattct tgctgatttg aagttggctg
    12181 atgctttttt tggtattgaa actggtattg aacactctat attggacaat gatgaaaatg
    12241 gaagagctta tctcagggga gcattcttat caacgggtac tgttagagaa cctgattctg
    12301 gaaaatatca actagaaatt ttttctgttt atctagacca tgcacaagac ttagctaatc
    12361 ttatgaaaaa atttatgtta gatgcaaagg ttattgaaca taaacatgga gcagtaactt
    12421 atttgcaaaa agcagaagat attatggatt tcttgatagt cattgatgcc atggaagcta
    12481 gagatgcttt tgaagagatt aaaatgatac gtgagacacg taatgatatt aaccgtgcta
    12541 acaacgttga aacagctaat attgctagaa caatcacagc tagtatgaag actatcaata
    12601 atattatcaa gattatggat actattggtt ttgatgcact gcctagtgat ttaagacaag
    12661 tagctcaggt gcgtgtggct catccagatt actctattca acaaatagca gacagtttag
    12721 aaacgcctct gagtaagagt ggcgttaacc atcgcttacg gaaaataaat aaaattgcag
    12781 atgaattata gaaggagata ttagcatggc ttgtacaaca atattggttg gtaaaaaggc
    12841 ttcttatgat ggttcgacta tgatcgctag aacggaagac tctgttaatg gcgatttcac
    12901 acccaaaaaa ttaaaggtaa tgacatctaa agatcaaccg cgtcattaca aatcagtttt
    12961 atcaaatttt gaagtagatt taccagataa cccacttcct tatacttcag taccggacgc
    13021 attgggaaaa gatggtatat ggggtgaagc cggtattaac agtaaaaatg tagcgatgag
    13081 tgctacagaa actattacaa cgaattcccg cgttttgggt gcagatcctt tggtttcaga
    13141 tggtataggg gaagaggata tactcacttt agtgcttccc tatattcagt cagcgcgaga
    13201 aggtgtggag cgtttaggtg ctattttgga aaaatatgga acctatgaat caaatggtat
    13261 tgctttttca gataccgaag aaatatggtg gttagaaaca attggtgggc atcattggat
    13321 tgctcgtcgc gtacctgatg atgtttatgt tactaatcct aaccaactag gaattgatca
    13381 ttttgaattt aataactgtg atgactacat gtgctctagt gatttgaaag agtttatcga
    13441 acaataccat ttagatttga cctattctaa tgagcatttc aatcctcgat atgcttttgg
    13501 tagccaacgt gataaagatc gtcattacaa cacaccaaga agttgggcaa tgcagcgttt
    13561 tttaaatcct gaaattgaac aggatccacg tagtttgttt attccctggt gtcaaaagcc
    13621 ttaccgaaaa attactgttg aggatattaa atatgtgttg agtgatcatt atcaagacag
    13681 tgtgtatgac ccatatggac cagaagggga tgcggtaagt aggagagctt ttcgttcagt
    13741 tggtatcaac cgaactagtc aaacgtctat tctacaattg cgaccaaata aatcacttga
    13801 aacgacaggt gttcaatggt tatcttatgg ctctatgcca tttgcaacca tggtgccgtt
    13861 gtttacacaa gttgagactg taccagacta tttttcgaat acaaccaagg atgcttcaac
    13921 agataatttt tattggacca atcgtttaat tgcagctcta gcagatccac acttttatca
    13981 acatgaagct gatattgaaa gctatatcga gagaacgatg gcccaaggac atgcacatat
    14041 taatggtatt gatagagaag ttgctgagaa taaagagatt gattttcaac agaaaaatca
    14101 agaaatgagt gactatattc aaaaagaaag ccaagaattg ttaaatcgta ttctatttga
    14161 tgcaagtaat ttaatgacaa atcgcttttc aatgggagat taaaaaactc ggaatatttt
    14221 ccgggttttt taagataaaa acgcttgcat aagtatataa aacttgcaaa aaaaaaatat
    14281 cgtgctatac tttactagta aaatattaac cggtaaataa cgggttaaat aaaaaggaga
    14341 aaaaatgaga aagaaatttc ttttattgat aagctttgta gctatgtttg cagcttggca
    14401 acttgttcaa gttaaacaag tttgggctga tagtaaactt aaagtggtaa caacttttta
    14461 cccagtttat gagtttacaa aaaatgtcgt tggtgataaa gctgatgtat ctatgttaat
    14521 taaagcaggt acagagccgc atgattttga accatcaact aaaaacatcg ctgccatcca
    14581 agattcaaat gcttttgttt acatggatga taacatggaa acttgggctc caaaagtagc
    14641 taagtcagtt aaatccaaaa aagtaacaac tattaaaggt actggcgata tgttacttac
    14701 taaaggcgtc gaagaagaag gtgaagaaca tgaaggacat ggtcatgaag ggcatcatca
    14761 tgaacttgac ccacacgtat ggttgtctcc agaacgtgcg atttctgttg tagaaaacat
    14821 ccgtaataaa tttgtcaaag cttatccaaa agatgcagct tcatttaata aaaatgcaga
    14881 tgcttacatt gcaaaattaa aagagcttga caaagaatac aaaaatggtt tgtcaaatgc
    14941 taaacaaaag agttttgtga ctcaacacgc agcgtttggt tacatggcgc ttgattacgg
    15001 tttaaatcaa gttccaattg ctggtcttac tccagatgca gaaccttcat caaaacgttt
    15061 aggcgaatta gctaaataca tcaagaaata taacatcaac tacatttatt ttgaagaaaa
    15121 tgcttcaaat aaagttgcta aaactttagc agatgaagtt ggcgtgaaaa cagctgtgct
    15181 tagtccactt gaaggacttt ctaaaaaaga aatggcagct ggcgaagatt acttctcagt
    15241 tatgagacgt aatttgaaag ttcttaaaaa gacaacagat gttgcaggta aagaagtagc
    15301 tcctgaagaa gataaaacta aaacagttga aacaggttac tttaaaacta aagatgttaa
    15361 agaccgtaaa ttgacagatt actctggtaa ttggcaatca gtatatcctc ttcttcaaga
    15421 tggaacactt gatccagttt gggattacaa agctaaatct aaaaaagata tgactgctgc
    15481 agagtacaaa aaatattata cagcaggtta caagactgac gtagaatcaa tcaagattga
    15541 tggtaaaaaa caccaaatga cctttgtacg taatggtaaa tcacaaacat ttacatacaa
    15601 atatgcaggt tacaaaatct taacttataa aaaaggtaat cgtggagtac gttatctctt
    15661 tgaagctaag gaaaaagatg ctggtcaatt caaatatatc caatttagtg accatggtat
    15721 taaaccgaat aaagctgaac acttccatat cttctggggt tcagaaagcc aagaaaaatt
    15781 atttgaggaa atggaaaact ggccaacata cttcccagct aaaatgtctg gacgtgaagt
    15841 tgcccaagac cttatgtctc attaatattt aaaagatgat atcggatacg atatcatctt
    15901 tttttcttcg tacgtgatag gctcaaacat agaaaaaagc acttctatta gaagtgcttt
    15961 taaaattaat gctatgctta ttgagcagcg tttgcgtctt tgagaccata ttttttgttg
    16021 aaacggtcca cacgtccatc tgcttgagtg aatttttgac gtcctgtata gaatgggtgt
    16081 gaatctgatg agatttccac acggataagt gggtaagttt caccttcgaa ttctactgtt
    16141 tctttagttg atttagttga accactaagg aatttgtaac ctgtagttgt atctaagaaa
    16201 acaactggac gataatctgg atggatgtct tttttcatct ttaaaaattt cctttctgcc
    16261 ataactcgtc tgagtcatag ttattaacca ctctattcta tcaaataaga atagccttga
    16321 caagtgtttt gataaaaaaa gtaaaattat aaagaaagta cttcctggat ttcttggtaa
    16381 atagcttgat tctcttccaa actataagaa ttagctccac tggctaaagg atggccgcct
    16441 ccattatgac gtttagctat ttcattaatg atgtgtgact tactacgtag gcgcacacga
    16501 taatgcccat cgctttgttc aacgaaaatt gcccaacttt ctacaatatc aatttttcca
    16561 ggtataccaa cgattgcagc agtctctgca tctgttacat caaagcgttt taagtcttct
    16621 tgtgttaaag taacgcatgc agcgccgttc ttgtcaattt caagttgctc aaaaataaag
    16681 ccttgtaatt ttgcaatttt gaaaggaaag gaatccattt gacgtgccat tgcagaaaaa
    16741 tcaaagtcaa attcacgaag tttgctagca atcttcagtg ttttacttgt ggtagcagga
    16801 tataaaaagc gtcctgtatc tcctacaata ccattatata gaactcttgc tgcacttgtt
    16861 gataacaaca aatcacaaga aagagcaaag tctgtcacaa tttcacttgc actagaggcg
    16921 ttagtatcta cataagagag gtctccatag acttcatcgt ttggatgatg gtcaatttta
    16981 attaaaaagt cacctttttt ataacgttca tcatcaatgc gaggggtatt tgctgtatct
    17041 gtaacaataa caagagcgcc ttgatagtct tggtcggtca cttgatccat tttagcaatc
    17101 caagcgagtg tgggttcgtc aaaaccagta gcaagtactt ttttctttgg gaaattatgc
    17161 ctgatgatat cccttaagcc gatttggcta ccaagagcat cagggtctgg tctcatatgt
    17221 cgatggataa tgatggtgtc gtactctttg attttatcaa gaatttgttg aaaaataatc
    17281 attttttacc tacctaactg ttttgtcttt tagtgaagaa caaggctata ttgttgacta
    17341 tattatagct taaatctttt attgaggaaa gaaattaaga tagcggccgc aatgtttgtt
    17401 tatttttata tgatggtatg ttattttttt ataaaattcg gatactgcta actaacaatc
    17461 ttttttatga taatattttg taaatgtatt gctttttagc taacttttgt taatatagta
    17521 aaaatgcttt ttaataggag ataaattatg acacaagcag tattaaaaga attggctaag
    17581 gcggagttgc attgtcatct agatggctca ctttctttac cagctattag aaaattggct
    17641 aatatggctg atataatact gccaaatagt gataaagaat taagaaaata tgttatagct
    17701 ccagctcaaa cagaaagctt ggtagattac cttaaaacgt ttgagtttat cagaccgctt
    17761 cttcaaacga aggaagcatt acgttttgct gcttatgatg ttgctagaca ggcagcttta
    17821 gaaaatgtta tttatattga aattcgtttt gcgccagaac tctctatgga taaaggttta
    17881 accgcaagtg atacagtctt tgcagtctta gaaggtttag cagatgctca gaaagaattt
    17941 aatatcgttg cacgcgccct tgtctgcggt atgcgccaat cgtctcataa aacgaccaag
    18001 gaaattatta aacacattgt tgatttggct cccaaaggcc tggtaggttt tgattttgca
    18061 ggtgatgaat tttcctatcc aacagatagt ttagtagatc ttattcagga agtaaaacgc
    18121 tcaggttacc cgatgacttt acatgcaggt gagtgtggtt gtgctaagca tattgcggat
    18181 agtttaaatt tagggattaa gcgtatggga catgtgacag ctttaacagg acagagggct
    18241 ttaataaagc gatttgtcga agaagatgct gtagcagaga tgtgtttaac tagcaattta
    18301 caaacgaaag cagcctcaag tattcaaagt tttccttatc aagaacttta tgatgctgga
    18361 ggtaagatta caattaatac agataatcgt actgtttccg atacaaattt gacgaaagaa
    18421 tatagtcttt tcgtaactta ttttggaacg aaaatagaag actttcttgt ttttaatcaa
    18481 aacgctgtga aggcctcctt tacaagtgat tcagagaaag atactttact acataagcta
    18541 caagaaaact atgacagcta tctaaaataa ccttcacgat tctttaaaaa tatgatagaa
    18601 tagtggtaat ttacaagtgg aggaagatat ggctttagca aagattgttt atgctagcat
    18661 gaccggaaat actgaagaaa ttgctgacat tgttgctgat aaattacgtg acttgggtct
    18721 agatgttgaa gtagaagagt gtacgatggt tgatgctgca gattttgaag atgctgacat
    18781 tgcaattgtt gccacttata catatggtga tggagattta ccagatgaaa tcgtggactt
    18841 ttatgaagat ttagcagaag ttgatttatc tggtaaagtt tacggcgtag tgggttcagg
    18901 tgatactttc tatgattact tctgcaaatc tgttgatgag ttcgaggctc aattcgcatt
    18961 aacaggagca caaaaaggtg ctgattgtgt taaagttgat ttagcggcag aagacgaaga
    19021 cattgaaaac ttagaagctt ttgctgaaga aattgcttct aaacttaact aagctaccaa
    19081 aaagacgaac acgagtcgtc tttttaactt ttttaccatc ttttgtaact agaatatgag
    19141 atatagatat ggaattagaa gagattcgtc aagaaataga tgaaatagat cagcaattgg
    19201 taagcctttt agaaactaga atgggtctaa ttttagaggt catagcattt aaaaagaaac
    19261 accgacttcc agttttagat aataatcgtg aaaacgaagt gcttaacaat gtattgaaga
    19321 aagtccaaaa tcatcaattt gacgatgtga tacgagcaac tttcaaagac attatgacag
    19381 aatctcgtgt ttatcaaaag gagaatattg tagacggtga ttaaacgatt tgataattat
    19441 gctcagtacg tacttgtttt agggtttagc ttactaatcg gtggtgtggt tggggctatt
    19501 acagcagtct ttggacgtgt tctattattc ctgacagcct ttagaagtga ttatatagct
    19561 tacctactcc cttttcttag cattgttggt ttatttatag tttttgtcta tcaaaaattt
    19621 gggggtaaga gtgttaaagg gatgggattg gtatttgaag taggtcatgg taatgaggag
    19681 actattccga aacgtcttgt tcctctcgtg atattaacaa cttggttaac ccacttattt
    19741 ggtggtagtg caggtcgtga gggggttgct gtacagattg gggctaccgt ttcacactac
    19801 tttcaaaaat attgccgttt acaaaacgct tctcaacttt tcctagtaat gggaatggca
    19861 gcaggttttg ctggtttatt tcagactcct ttagctgcta ctttttttgc gatcgaagtt
    19921 ttggtagtag gtcgtttaat ggtttcgtat atattaccaa gtttaattgc tgctctgaca
    19981 gcaaatttcg tctcacattc tttaggttta gaaaaatttt ctcatagtat agcaacatca
    20041 atggcgttaa caccagatat tattctaaaa ttattggttt taggactatg ttttggtctg
    20101 tgtggcaatt tatttgctta tttattggct aaagctaaat taattgcttc tagtaggtta
    20161 ctaaacccat ataaacggat tttcacactt ggattgttag ctactttttt attgtttatc
    20221 ttccattttg gacgatattc aggactggga acaaatctaa ttgaggctag cttcactaat
    20281 aagaatcttt atgattatga ttggttgtta aaattgtgtt tgacagttat tactttggct
    20341 gcaggttatc agggaggaga agtaactccc ttatttgcaa taggagcaag tttaggagtg
    20401 attattgcgc ccattttagg tttgccagtt attttagttg ctgctttagg ctatactagc
    20461 gtttttggga gtgcaacgaa tactctgtta ggtcctatct taataggtgg cgaagtcttt
    20521 ggctttgcca acactcctta ttttgttatt gtatgtcttg ttgcttactc catatcacat
    20581 gctcatacga tttatggtgc tcaatctaga tagttagtga tgagttttaa aagagtttat
    20641 cagtttatag aacggaatat tccgttctct tttttattct cttaaagttt ataaaaatga
    20701 agtttgtaaa ttttatgaat gcaaaaatta tgtggctaat ataattttct tcaaagaaat
    20761 tgattttatg attgtcatta aaatactgta aaatccaatt gaatgtgtaa gaacttaggt
    20821 taggataatg tctacaagca tttaagtcgc gatggtgaaa tgcattagaa gataaaaagt
    20881 tgatgtggat ttcaacttta tgggaaagag tcaggtgaca cgaaagctaa tatagaaaac
    20941 gtagtttaaa aaatcatata atattaaatg aattaaactc tcaaaaatca gaaaaaagct
    21001 aacctagaaa cagtaaatca aatttcaata atatacaaat aactattcta aaggatgaca
    21061 ccttgttctt ctagataaaa tagctttaca agcaggttaa aatttggtag aattgtgaga
    21121 gtgtgtaatg gacgcacaaa aaacggctaa tccgctgaga caagtactta agattagtaa
    21181 gagaaggaga ataaaaatga atccattaat tcaaagtttg acagaaggtc aacttcgttc
    21241 tgatatccct gagttccgtg ctggtgatac tgtacgtgtt cacgctaaag ttgttgaagg
    21301 tactcgcgaa cgtattcaga tctttgaagg tgttgttatc tcacgtaaag gtcaaggaat
    21361 ctcagaaatg tacacagtac gtaaaatttc tggtggtatc ggtgtagagc gtacattccc
    21421 aattcacact cctcgtgttg ataaaatcga agttgttcgt tatggtaaag tacgtcgtgc
    21481 taaactttac tacttacgcg cattgcaagg taaagctgca cgtattaaag aaatccgtcg
    21541 ttaattttga tgatcagatt ttaaaaatgc ttggttgttt gaggatagta actatgtttt
    21601 aaaactggac aaccaagacg taaaaaatct gcctgtgggc agttttttta ctaggtcccc
    21661 ttagttcaat ggatataaca actccctcct aaggagtaat tgctggttcg attccggcag
    21721 gggacatatt cattgcatgt aaatagcggt ttagagctat tttgccccaa atccgcccca
    21781 aatttctctg attaagttta tcgttcctat ctttttgttc ttgtaattga tgtgcgtaaa
    21841 cttctaaagt gatatttaaa ttctcgtgat ctaaaacttg agagatggaa attagatagc
    21901 ttgcaaatgt atgcctgaga gagtgcactc gtacctcgcg accagttatt tttcggatag
    21961 ttttattgac tgcattattt gaaagtttgt cgaataatct gtcgttttta ttttttgtaa
    22021 attcatgcaa aaaaaataat gtatcattgt caattggtat atttctgata ctacttttgt
    22081 tttttgttgg caggtatctt tggttgaaat gataatccca agttttatta attgataaat
    22141 atttgttagt gtaatcaata tcattaactg ttaaacctaa acattcagcg aagcgcatgc
    22201 cagttttagc gatgaggtat aacgctgcat acgattgatg ttgtgatttt tctttacaaa
    22261 tttttatcaa gcgtaagtat tcattggttt caagaaattt tatctttatt tacgcccctt
    22321 attttttgct ttaaccttag tgaataaaca aaaatttttt tctatatatc cctcgtgaac
    22381 agccatggat acgcaggctt ttacatgtat gttaaaacgc tttactgtat cttgcacatg
    22441 cgtttgacta taatgattta tgacttgttg atatttagtg gaagtaatat tgcaaagtaa
    22501 tatatttcct attatatgtt tatacgatat tcgatattcc cacccgttgt cgcgtttacg
    22561 gaaatacgcc attgatatac tccacattag ctaaagaaca gggtgttcaa ggctaccttg
    22621 atggaaaagg ctctcttaga gatatttgta aatggtatga tatctcaagt cgctctgttc
    22681 tccaaaagtg gataaaacgg tatactagtg gtgaagactt gaaagccact agtagaggat
    22741 atagccgtat gaaacaagga aggcaagcca catttgaaga acgtgtagag attgttaact
    22801 acaccattgc ccatgggaaa gactatcaag cagctattga gaagtttggt gtttcctacc
    22861 aacaaattta ttcttgggtg cgtaagcttg agaagaatgg ctcacaaggt ttggttgata
    22921 gacgtgtgaa agggttggag agtaggcctg atttaaccga gattgagcaa ctttaactca
    22981 agattaaaca attggaggaa cgtaatcgtc tcttagaaat cgaggttagt ttactaaaaa
    23041 agttagaaga catcaaacga ggaaacagac ggtaagacta ggtaagcatt tagcggagtt
    23101 ccaagtaatc aagaattatt acgatgagga atctaatgtg cctattcagg ccttatgcca
    23161 actcttgaag gggtctcgtt caggctatta caagtggctc aatcgtcaaa aaacagattt
    23221 tgagacaaaa aatacaaagc taatggctaa aatcaaggaa cttcatagac tctacaatgg
    23281 tatcttaggt tatcgccgta tgacaacatt tattaatcgt caacttggga caacttaaaa
    23341 caagaaacgg attcgttgat tgatgaacat tctggggatt agttcagtca ttcgtcgtgt
    23401 tagccatgct tgtacaaaag ctggtgacag attttacgaa gaaaatattc ttaatcgtga
    23461 atttacagcc acagctcata accagaaatg gtgcacagat gtcacctatc ttcaatacgg
    23521 tctgggagct aaagcttatc tcagtgcgat taaagacctg tataacggtt ctattatcgc
    23581 ttatgagatt agtcacaaca atgaaatcca cttgttatga agaccattaa aaaggggcta
    23641 gagctcaatc caggagccac acctatcatc catagcgatt gaggtagtca atatacttcc
    23701 aaagaatacc gttatatcat acaacaagct ggtctgacct tatccatgtc ccggattggc
    23761 aaatgtattg ataatgcacc aactgaaagt ttctttgggt ttttcaagac tgagtcttac
    23821 caccttaaga aatacaactc ttatgatgag ttggtcaatg atgtggcacg ttatatcgaa
    23881 ttctacaaca cacaacgtta tcaatcaaaa ttaaacaatc tgactcctct agaattcagg
    23941 aatcaggttg cataacttat cttttattat ttgactgtct acttgacagg gagccgttca
    24001 gattgcttaa cctttctaaa tttgctaaaa tagctacaag aaaacgagcc atttaatgct
    24061 tatttcttat actgtcttgc ctcacgctct cctcgaccaa aaattgagcg tgaggctttt
    24121 tgtttcatta aacgatgata tttccatatt catcagtttg ttttccgaga gccatcaaag
    24181 cttcgataag gtcgataatt ccaggaataa aggtaatact aaaaataata tataaaaaaa
    24241 cctggcctat ttttcctgcg taaaatttat gcgctcc
//
LOCUS       DATRSG010000025        24251 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRSG010000025 DATRSG010000000
VERSION     DATRSG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24251
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.025"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            21..218
                     /locus_tag="VBD89_000996"
     CDS             21..218
                     /locus_tag="VBD89_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135538.1"
                     /translation="MNRLREYRIKASISQLALAKSVGVARQTINLIENNKYNPSLDLC
                     IRLAECLQTDLNTLFWDSRHL"
     gene            318..851
                     /locus_tag="VBD89_000997"
     CDS             318..851
                     /locus_tag="VBD89_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO7135539.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
     gene            complement(927..1421)
                     /gene="tpx"
                     /locus_tag="VBD89_000998"
     CDS             complement(927..1421)
                     /gene="tpx"
                     /locus_tag="VBD89_000998"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO7135540.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(1494..2153)
                     /locus_tag="VBD89_000999"
     CDS             complement(1494..2153)
                     /locus_tag="VBD89_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO7135541.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            complement(2153..2917)
                     /locus_tag="VBD89_001000"
     CDS             complement(2153..2917)
                     /locus_tag="VBD89_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7135542.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            complement(2922..3563)
                     /locus_tag="VBD89_001001"
     CDS             complement(2922..3563)
                     /locus_tag="VBD89_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7135543.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            complement(3727..4278)
                     /locus_tag="VBD89_001002"
     CDS             complement(3727..4278)
                     /locus_tag="VBD89_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7135544.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            complement(4300..4497)
                     /locus_tag="VBD89_001003"
     CDS             complement(4300..4497)
                     /locus_tag="VBD89_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7135545.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            complement(4544..5086)
                     /locus_tag="VBD89_001004"
     CDS             complement(4544..5086)
                     /locus_tag="VBD89_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7135546.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            complement(5142..5336)
                     /locus_tag="VBD89_001005"
     CDS             complement(5142..5336)
                     /locus_tag="VBD89_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO7135547.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            complement(5346..5927)
                     /gene="amaP"
                     /locus_tag="VBD89_001006"
     CDS             complement(5346..5927)
                     /gene="amaP"
                     /locus_tag="VBD89_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO7135548.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            complement(5942..6190)
                     /locus_tag="VBD89_001007"
     CDS             complement(5942..6190)
                     /locus_tag="VBD89_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7135549.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            complement(6238..6483)
                     /locus_tag="VBD89_001008"
     CDS             complement(6238..6483)
                     /locus_tag="VBD89_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7135550.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            complement(6895..9174)
                     /gene="pcrA"
                     /locus_tag="VBD89_001009"
     CDS             complement(6895..9174)
                     /gene="pcrA"
                     /locus_tag="VBD89_001009"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO7135551.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            complement(9280..9666)
                     /locus_tag="VBD89_001010"
     CDS             complement(9280..9666)
                     /locus_tag="VBD89_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO7135552.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
     gene            complement(9799..11124)
                     /locus_tag="VBD89_001011"
     CDS             complement(9799..11124)
                     /locus_tag="VBD89_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO7135553.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     regulatory      11432..11564
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11721..13067
                     /locus_tag="VBD89_001012"
     CDS             11721..13067
                     /locus_tag="VBD89_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO7135554.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLLGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLALSPIIVKETQKYFSETKNITKP"
     gene            13131..14366
                     /locus_tag="VBD89_001013"
     CDS             13131..14366
                     /locus_tag="VBD89_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7135555.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVCFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITEKVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQITSLD"
     gene            14525..14917
                     /locus_tag="VBD89_001014"
     CDS             14525..14917
                     /locus_tag="VBD89_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7135556.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPIPGSIIGLILLFLALKY
                     KIIRLRHIDTVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            14898..15593
                     /locus_tag="VBD89_001015"
     CDS             14898..15593
                     /locus_tag="VBD89_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO7135557.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLKIDDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            15662..16285
                     /locus_tag="VBD89_001016"
     CDS             15662..16285
                     /locus_tag="VBD89_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO7135558.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(16554..16625)
                     /locus_tag="VBD89_001017"
     tRNA            complement(16554..16625)
                     /locus_tag="VBD89_001017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16590..16592),aa:Arg,seq:ccg)
     gene            complement(16698..17900)
                     /gene="rpsA"
                     /locus_tag="VBD89_001018"
     CDS             complement(16698..17900)
                     /gene="rpsA"
                     /locus_tag="VBD89_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO7135559.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(18026..18097)
                     /locus_tag="VBD89_001019"
     tRNA            complement(18026..18097)
                     /locus_tag="VBD89_001019"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18063..18065),aa:Gln,seq:ttg)
     gene            complement(18103..18183)
                     /locus_tag="VBD89_001020"
     tRNA            complement(18103..18183)
                     /locus_tag="VBD89_001020"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18147..18149),aa:Tyr,seq:gta)
     gene            complement(18244..18474)
                     /locus_tag="VBD89_001021"
     CDS             complement(18244..18474)
                     /locus_tag="VBD89_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO7135560.1"
                     /translation="MSKKDKKIEIQILDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            complement(18557..19579)
                     /locus_tag="VBD89_001022"
     CDS             complement(18557..19579)
                     /locus_tag="VBD89_001022"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO7135561.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            complement(19693..22152)
                     /gene="parC"
                     /locus_tag="VBD89_001023"
     CDS             complement(19693..22152)
                     /gene="parC"
                     /locus_tag="VBD89_001023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO7135562.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIADYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYVLRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            complement(22286..24235)
                     /gene="parE"
                     /locus_tag="VBD89_001024"
     CDS             complement(22286..24235)
                     /gene="parE"
                     /locus_tag="VBD89_001024"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO7135563.1"
                     /translation="MTKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
BASE COUNT         7745 a         4812 c         3717 g         7977 t
ORIGIN      
        1 tactaaagga gctaataatc gtgaatcgtc ttagagaata cagaattaaa gctagcatat
       61 ctcaattagc tttagcaaaa tcagttggtg tcgccagaca aacaataaac cttattgaaa
      121 ataataaata caatccttcg ctagatcttt gtataagact agcggaatgc ctacaaacag
      181 acctcaatac tcttttttgg gatagtagac atctataaca gattagactc tctaaatcta
      241 tgaaaactcg ggaggttagg tcgtattatt atttcaatct caaactttat taaatagttc
      301 aaggatagga gaatattatg aaagaaaaga aaaatttggg gataaaatta ataaaatccc
      361 tatatggtat taatggccca ttcgatgaat acagagaaca aaaaatcaat taccttggaa
      421 atctattatt tgcaaccact agtctctatc acattttatc ctctatcatc gcctggttca
      481 cggtaagtaa atatcccaca gagactgcat gtactctcct agctttaaca ttctttctat
      541 atttttgtat ttttctaatt gttattccct cttatctcaa cagtcatgat ttaataacgc
      601 cactagaagt ctcaaaaaat gactatccag tagcaataaa gaaaaacaaa caaaaattta
      661 tcaaaatcgg gatattttat actgtcatcc agtggcttat cttgggttta acaactaaca
      721 ataacactcc gctgattgta tcactatttt ctttaaaagt tattttgtct agtattatgt
      781 ccggtgtagc ttacaatgga gttatgtatc taatatttaa aaacaatgtt gatattgaaa
      841 aagatgaata atgacaacat ttcatctcta attgttctca ctgtctatat aaataataag
      901 gctggaaaat tttccagcct tattatttat ggtaatatct ttgcagcatt aatcgccgct
      961 tcgtagtcaa catctgaatt aacattgtca acgtattcgg tgtaagtcac cttattatgc
     1021 tcatccaaaa taagaactgc acgcgttaac aaatgccact cattcatcaa aagagcgtac
     1081 tcttgaccaa acgaatgatc ataaaaatct gataataaaa tcacgttatc aagaccttta
     1141 gcgctgcacc aacgcttctg agcaaatgga aggtccatag aaacagtaat aaccactgtg
     1201 ttatctagct ctgataattc ctcattaaaa gtacgtgttt gttttgaaca aatacctgta
     1261 tcaatagaag gaacaacgct aataactttc tttttccctt caaaatcttt taaagatttt
     1321 tgagataagt ctgtagctat tagtaaaaaa tcttttgcaa tatccccaac ttgtagctgt
     1381 tttcctgtaa aagtaacagg atttccaaga aatgtcgtca taagtttatc tccttatcaa
     1441 ttacctttat tttactatga agcttcttct ttttcgaata attgaactga taattagagg
     1501 tacttacttc cttcgcgtat gctcaaagtc gacaactcat cataatgatt actgataaag
     1561 tcctttaccc atactttatt atactttgaa taatccctaa gcgcccagcc aatcgcctta
     1621 ttgataaaaa attcttgact tcctgtattt cttaaaataa aaagtgacaa aatatcagta
     1681 tttgtttttt ctttaaatcc tagttgatgc tcaatagcta gtcgcctcat ccaaaaatct
     1741 tgatcaagtg accattctga cagtaaattc ataaccttgt catcttttaa ggataagttc
     1801 ccaaaaatct tagtcagaac atcaatacta tcccaccact gttgatgacg agcgtaaaac
     1861 tctagtctag agcaatcatt ataggtgaga aattgactca tagctaatag ataatcaagt
     1921 acaaaatgat gatattcacg atagtcactt tgccaacatt tatcaagcaa ttgccaatca
     1981 atttttgctt gtcttttagc ggcctttata agctctttgt agagcatacg tcgctcaggt
     2041 gatttaatac catagcactt aaaatgatgg cgtaaataag cctcctgttg cttagaaact
     2101 tctttgtcac ttgcagcttt aaatttcctc tctaaactta ggaataaatc cattacgtaa
     2161 tccccttgta tttttcaaaa agcattgtaa ttccttcatg tacagattct cttgatacac
     2221 gtaaattagc gtaaggagtc agttcatcaa aatcccctac ttgaacacta taatgcgcag
     2281 catcaaacat agaaatatca tttttatcat tgccaaaagc tataaaacga ttcccaaata
     2341 atttcttagt ggcttttccc ttgtcaactg caatgggatt tatgtacaaa catttttcaa
     2401 attcgtggaa aaagtgacta ctaagtccaa aacgatttaa tttcgctaac atttggtctg
     2461 cccttctcag ctgatcacca aaatacaaca ccatttttat cggtttctca attttagaaa
     2521 cttctagaag ctctcccctt ttttgaggat caacataagc aataaaggga attttagaag
     2581 cctgataagt agcgtaatta aagacctcat caacaaaata tggaatctga tagcgatgac
     2641 aatatgaaat aatagtacta aaaaagtcac tttgtaaata gtaactatcc actaagtgac
     2701 cattttcatg taaagtcgca ccatttaaac caataatttt tgataaagat agcttgtcgc
     2761 caaggatacc tatagtgtct ctataagatc gcgccgtagc aaaagtgact ctatgaccat
     2821 aatcaatctg ggctctctct agtattcctt gtatttcttt agatagactc ataccatcaa
     2881 aacaaagagt tccatctata tcaaagacaa aatccattct ctcaaagccc catccttaag
     2941 ttaaaatagg ttttcatacg tgcataaaca tcttcatcac caacaaaaaa gatatgatct
     3001 ccttgctcta taactgagaa aggccctgga gaaacaataa atttcccttc gtgttcaata
     3061 gccacaatag tcgcaccagt ttgatgccat acatttaatt ctcctattga cttaccaagg
     3121 tgttcagaat cttcactgac aataatttca tatggtgcta gtgggtattg tttactaacc
     3181 gcacgtgttt gaagcaaaaa atcatcaact aaagtagcta actcttccat ttcttgttgc
     3241 tgagccttaa tattatcacg gattttacct ttcaaaatag caactgaatg agaggtttca
     3301 tattggttta agaatgcaat tgctttttct ttagaaagaa taatggctcc actgccatgc
     3361 tttaaagtta aaatctgtaa atctgctaaa atattaagac ccttacgtgc tgtttctggt
     3421 gatacgttga atgttgaagc aatagttgtt ctagatttca acttctctcc aacttcataa
     3481 tctccatttg cgattctttg tgcaaccgct actgctattt tttgatattt agatgttaca
     3541 atttcagatt gttcagaaac catccaaccc tccttatcct aataaaaaca tacatattaa
     3601 accacaatta gcaaaatatt gcaaatatat cttttttgtt aatatctaat gtctttagta
     3661 aatatcatat aaaaggagct ggtctcccag ctccgagtgt cttatcataa aaagtagttt
     3721 actgaattaa gattcactag tacgagcttc tgagattttt tcttttgcag cctcagtacc
     3781 atttgatact gcttcttttg ttttctctgc tccagaagaa attgctgctt tagctttgcc
     3841 agcttgttca gaagcaaagt ttcctgttgc ttgtgctgca tcagatacac gatcttgtac
     3901 tgtcactgag tcagcttcat gttcagcttt agtcttaaca tctacgacat ttgcgttcaa
     3961 ttcaacaact ttaaggtggg tcatcacttc aacgttttga ctgacaatcg ctttaatgct
     4021 ttcaacgata gctgggatat ccttgccgta ttcaacaacg atgtctaaat caactgcaac
     4081 ttctttagta ccaacttcaa catttacacc atcagttact gagtcagaat ttacaacgct
     4141 attttttaga ttagagaaga aacctccact cacagccaag agtccgtcta ctgattctaa
     4201 agcatgtcca acaattttag caattacctt ttcttcataa gttactgaag ttttaatatc
     4261 ttgtttttga ttttccataa ataaatgacc tcaaaccttc tagtcctttt tcttaaagct
     4321 ctctgaagca cccttaatgg tatctttaat gtcagcaagt ttttcttttg catgaccttt
     4381 caatttatcg actttacctt cagattcaac ttctttatcg ccagtaagtt tcccaacaga
     4441 ttcttttact gaaccagaaa ccttatcaac ttttgcatca aatttttctt cagacataat
     4501 agcctcctac taataacaat actttaaact atacttaaat caattattgt acacgtggtt
     4561 cagacttcat gtcttccact ttatcaacac cgttacctac ggcactttta acgtttgaaa
     4621 cttgattcga agcaaattca cctgttgctt gtgctgcact tgttacacga tcttgaagag
     4681 ttactgaatc ttcttcatgt tgagcacgag ttttaatatc aacaacatta acattaactt
     4741 caaccacttc aaggtctgtc atacgtttca cttcagcttc aacaactttt ttgatatcgg
     4801 caaagatagt tggaacatgt ttttggtatt cagcaacaat atccaaatca acagcaactt
     4861 gctttttacc cacttcaaca tttacgccat ctgtaactga atcagaattt acaacgctat
     4921 tttttaaatt tgagaaaaaa ccaccattta cagccaaaag accatctaca tgctcaatcg
     4981 caattccaac aattttttca atcactttat cttcgaaagt taattctcca cgaacagctg
     5041 tcgtgccaga attgttagtt gtgtttttga tgtaagtttc agtcataata gttcctcttt
     5101 cttttattta aataatgtag aataaatata aacgaaaaat tttattttct cttattaaag
     5161 aattggtcaa gcatacctgt tctttgcaca taaagcccag cataagcacc tagcacaatg
     5221 ataacaagag caagaattgt tttaaagaat ccaatggtta caatcattgc agctaaaacg
     5281 agcccaataa ctgcacctcc tagtggatat ctgtatttcc tcacgaattc tgacatatta
     5341 gactcctatt ctacacgact tttattcgtc ttagcatttc caacattttc ttcttcaact
     5401 tgcttaacaa atactctagt gttcattttc ttttccaacc caaagaattc ttttaagcca
     5461 agttcaacat cactttgaat atgttcaagt tgttccggta catttgtacg agaagttaaa
     5521 agacctttaa catcaacttt cacttttttc ttatataaat gagctgtcac actaggagat
     5581 ttcatcaaac cagtttttaa tacttctgtt ttaacaaatc cctctattgc agattttttt
     5641 aacatcaatt taccagtttt atcagccagt ttatactctg tgtatcgtct tggataaaac
     5701 agaactacca atatagccag taaaacgatt acaaacagca ctaatgctcc ccaaaagaaa
     5761 taatgataca taccaacgtc aatcggtgat ggcaaattaa aatcccaatc taaccaatta
     5821 aaagatggta gataaatgtg ttgtttactg ataacagcta caaatcctag tagggtaagg
     5881 gagattagac ctagcaaagt atataaactt tttagtcctt ttgacatagg taactccttt
     5941 tctagttatt cattttacct agtacaaatg aagtaacaat gacaacaata atcgcaccta
     6001 cgattgaagg aatcaaagcc atacctgcta atttaggccc ccatgttcct agtaaacttt
     6061 gaccaacaaa tgaaccaact aaaccagcta agatgttagc tatccaaccc attgaaccac
     6121 ctttgttagt aacagcccct gcaatagcac cgataatagc acctacgatt aatgaccaaa
     6181 ttaaactcat gataaatacc ttctctttct tgactaatca ttctactagt tatgtagtta
     6241 tgattttttg cctaatacaa atgaagtaac aatgataacg ataatcgcac ctacgattga
     6301 aggaatcaaa gccatatctg ctaacttagg cccccatgtt cctagtaaac tttgaccaac
     6361 aaatgaacca actaaaccag ctaagatgtt agctatccaa cccattgaac cacctttgtt
     6421 agtgattgct cctgcaatag caccaataat agcaccaacg attaatgacc aaattaaccc
     6481 catgataaat acctttcttc ctaagtacat ctcagtgtac aaacttattt tgttcttaac
     6541 taaataacaa gagtttctaa agtgaccact cggcaattaa ttaagttgtt aactatacta
     6601 atattataca actttataaa gtgactgcca agtaaaaagc ttggtaaatg cttttatttt
     6661 taagtgacca cttgatattt aattgatatt gttattatac tctttttaga aaagaatgca
     6721 agagaaacgc ttctttcttt aaaatagtat ggtcactttt ggaaaacgct ttaatatcaa
     6781 cctttttagt aagtaaaaac caaatgataa cttattttgt ctattcaaat gatgcaaaaa
     6841 gactaagaat acaattctta gtctctgatt atttaagcat ataatagaag aaagttagtt
     6901 ttccttttta ctaataggag cgacactcgc taataatttc tttaacccaa tacctggaaa
     6961 atttattttt aactcttgag tctttccgct acctgaaact tcaagcaccg taccgtcccc
     7021 ccatttttta tgagttgcaa catcaccaat ctcccatgat gtctcatggc tattgttcgc
     7081 gttaagagcc tggagttggg cagttacttg aggttgtgaa ttacttttac gtgcctgaag
     7141 cgcttgttga agactcattc cttgaccaaa ttgtgtaggc tgttcttttg aatatttcac
     7201 accaaatgaa gaattaactg gtcgagccaa cccttgatgt tgtatcagct catcatcaat
     7261 ctcacgtata aaacgggttg gtctattata actagtttta ccaaataaag tacgggtatt
     7321 agcattggta aggaaaagta tttgttctgc tctcgtaatc ccgacataag ctaaacgacg
     7381 ctcttcttct aattcatcag catcctctat tgcacgcgat aaaggaaata ctccttcttc
     7441 cataccgatt aagaaaacaa ctggaaattc tagacccttg gcagcatgta atgtcattaa
     7501 agtgacttca gccgtttcag tggcgctatc atcagtatct gctattaaag ctaaatcatt
     7561 taaaaaacgc gatagacgat ctaaaccagt ttcaccctct actgtaatct ctgggttatc
     7621 gtcaaaattt ttagtcactg ataagaattc ttcaatattt tcaatacgcg cttgactttc
     7681 taatgtattt tgaacttgaa gtgcttctag atatcctgtc ttatccagta ggttttctgt
     7741 aatctccgtt acagttaaac tatctagctt actacgtaga gtcaaaatca gattagctaa
     7801 atcccaaaca gcttgagcag ccttcccttt taagggcgat atcatgacat ttgaagagga
     7861 atcaagaaga gacatgttct gttcataggc aaatgaccgt attttttcta aagttcctgg
     7921 ccccacccct cttttaggtt catttacaat ccgctcaaaa gaaatattat cagaagtatt
     7981 tgcaaggata ttgagataag caataacatc tcgaatttct ttacgactat agaattttgt
     8041 tccaccaacc attgtatatg gaatattgga ttttaaaagt gcttcctcaa tagtacgaga
     8101 ttgcgcattc gtacgataaa gaacggcaaa atctttgaaa ttttttcctt gttctcgaac
     8161 gatgttatca atagttgacg ctacaaaaac ggcttcttct tgctcgttgt tagctctatg
     8221 atatacaatt tgctcacctt catcattttg agtccataat tttttaggac ggcgattttt
     8281 attatgattg atcacattat tagcagcttg aagtattttc ttagttgatc gataattttc
     8341 ttctaataat acaactttgg cttgcggata gtccttttca aaatcaagaa tattttgcat
     8401 atcagctcca cgccatccat aaatggattg atcggcatca ccaacaacac aaatattttt
     8461 gaaacgcgaa gctaataact taactaattg gtattgagcg tggttagtat cttgatactc
     8521 gtctacatgg atatattgat acctctgttg gtaataggcc aaaacatctt tattctgatc
     8581 aaataatcga agtgtcatca taatcaagtc atcgaaatcc atggcctcac ttctacgtaa
     8641 ttcctcttga taagctttat agcatttagc aattacctgt gtatacatat ctccggcttg
     8701 cttctcatat gcaatttcat caagaaggtc atttttagca ttagagattg tacctaaaat
     8761 tgaacgttca ttccactttt ttgtgtccaa atttaattgc ttaatgattc gcttcattaa
     8821 agtacgctgc tcacctggat caacgattgt aaagttacga ttatatccaa tataatcagc
     8881 ttcacgacgt aaaatacgaa cacacatact atgaaaagtt gcaatcaaag tatcttgggt
     8941 agctggattc aaggcgatag cacgttcacg catttcacgc gctgctttat tagtaaaagt
     9001 aatcgctaaa atattccaag gattaacata tttttcatct attaaataag caatacgatg
     9061 agtcagaaca cgtgtttttc cagagccagc tcctgccata atcaaaagcg gtccgtctgt
     9121 cgtttgtacc gcttctgctt gtttatcatt cattccaatt attaaaggat tcatttctac
     9181 tcctaatttt gtcaaaattg cctctctagt atatcatttt ttcatctttt tcccaataga
     9241 aaaaggagtt aaacagacgc tgtctaactc ctaccttttt tacttctctt ttctctctcc
     9301 agttacaaac attgtaaaaa tagcagttgt aaccatttcg tctttctgat ttttaatgtg
     9361 aacatcaacg acttttgtag tacgaccatt atgaacacaa gtaccgatga cagtaagcag
     9421 atcatctaat cggcctgccc tcaaatagtt gatatttgat tgtagagtta ccgcttcata
     9481 tcctgttgaa atagcaacta aaccactgac ttgatcacac agtgtaaaca aataaccacc
     9541 atgagcatta ccataatagt tgagcgaaga gtcaacgacc ttcgttgtta aggtaacttg
     9601 accttcttcc cagttttcaa tttcgtaatt ctcaaatact tttacttgtt ctaaattgaa
     9661 tttcattcta ctataacctc actttttcta tgaccattat agcagaaaat aaggaaacca
     9721 taactgtaaa cgtttttatt ttaaagtttt taactgatct atcataacaa aagagctcag
     9781 atactctgag ctcttttgtt atgatttcat gctttctttt ggcaatatta aatttaatat
     9841 aatacccact atagcactta atgctgttcc tgataaagtt agagcaccta gatcaaggac
     9901 tgctccaccg aggcctaaaa ctaacatgga actggcaata atcagattac gaacctcagc
     9961 aaaattgaca cgattttcaa tcaacacttt taatccatta cttgcaataa caccataaag
    10021 taatatagcc attccaccaa gtactgcact aggaattgta gagatcaaag ctgtaaattt
    10081 accaaagaat gaaaaagcga ttgcaataaa agctgcatta cgaataaccg ttacagaagc
    10141 tatacgcgtc atgccaataa cacctgtatt ttcaccataa gtagtttcag ctggccctcc
    10201 tataagagca gaaaatgcgg ttgctaaacc atctccaatt aaaagacgat ttaaacctgg
    10261 tttttttaga aagttacgcc cacaaatctg acctaaaact gtatgatcac cgatattttc
    10321 agcaatcgtt acaattgaga ttggaagaat tgcaagcatt tctggtccaa agtaaagttt
    10381 ataactgtga aataaacctg tcttaaacgg taaatagaat tttggtaatt caaaccaggc
    10441 agctttttca actggagata aatctaccaa gccaagaatg attgataaaa tataacctcc
    10501 aataattgca aataaaaatg gaataatctt gataaaacct ttccctttag tattgataaa
    10561 agctgcaatc aagaatgtaa caacagcaac tagcatttta cgccaatcac ctttagcgac
    10621 aaaacctgca ttagttacag cagagtttgc caaacctaaa ccaataacaa taatcatagg
    10681 tccaatgatt attggtggca aaatcttatc aacccatgaa tttcctacga acttaataac
    10741 cgtcgccaca acgacataga ttagacctac aaaaagaata cctgtttgag cagccgaaat
    10801 atcgccatgc atttgtttca tggctaatgc catagctgta atataagcaa atgatgagcc
    10861 tagataaaca gggactttaa attttgtcgc aacttgataa atgagcgttc cgcaaccaga
    10921 agcaaataaa gccactgata caggcatccc taaaataagc ggaactaata tcgtagctcc
    10981 aaacatggca aaaacatgtt gaaaacttaa aagcaaacct tgaaataatt ctggtttttc
    11041 atcgatatct agtaataagt tagtagaatg tacttcctta tctactagaa tttgattact
    11101 ttcaccttta gcatttaaag tcatatctaa actagtcctc ttgggcatac aaaaaagccc
    11161 actttctata gcaatatagt cataggcttt atttttttcc tgatgttttt ctcacctcac
    11221 gggatgagtt taaaaaccta cgctgaaaga attatagcat aagacatttt agaaaacaag
    11281 agttttttga aactatattg acaaaactat gctttttcat tatgatagtt aacataataa
    11341 aatataccga atgacgtcat tcaggagaag aaagttagac tttcgccgaa ggaattacac
    11401 tctcaggtgt cttaagacag gactgattga cagacggact tctggagaga cctataagta
    11461 gcaacatctt tgtattgaca ccaagatgtg ctctaggcgc cgaaggggca agaagagtaa
    11521 aacaactcct ccaatctctc aggcaaaagg acagaagcta aaagccaata ttaataatga
    11581 gtagtaagct tattaagttt actactacct ttatttgtgc gctttttagc tagcatcttt
    11641 cagaagttat ctcttttaga gataacttct ttcgtttcat tacagaatcc ataggtatgt
    11701 catgtatcaa aggagaacat atgctaacac tttttactca tatcaatagc ttcgtttggg
    11761 gtccaccttt acttgcttta ttagtcggaa caggtattta cctatcattt cgcttaggtt
    11821 ttgttcaatt gagacaactt tctagagctt tcaaattgat tttccgagaa gataatggac
    11881 aaggagatat ttcaagttat gctgctcttg caactgctct tgctgcaacg gtagggacag
    11941 gtaatatcgt tggtgtggct acggctatta aatctggagg accaggagct ttgttttgga
    12001 tgtgggtagc cgcctttttt ggaatggcaa caaaatacgc agaaggatta ttagctatca
    12061 aatatcgcac aaaagatact aatggagaaa tttctggggg accaatgtac tacattgtca
    12121 atggaatggg acaaaaatgg aaaccattag cagtcttttt ttctgcagca ggtatcttag
    12181 tagctctttt aggaataggg acattcactc aagtcaatgc aattgcctct tctcttgagc
    12241 atactttcaa aatatcaact agatttacca gcctcattct tgctgttata gtattattta
    12301 ttatatttgg aggtataaaa tctatttcaa aagtttcaga aaaaatagtg ccttttatgg
    12361 ctataagcta tattttagca actctgatta ttattgcagt taattacaat aagattccac
    12421 atactttcca actcatcttt agcggtgctt tttctgggac agcagccatt ggtggttttt
    12481 ctggagctat cgttaaagaa gcaattcaaa agggaattgc tagaggagtt ttctcaaatg
    12541 aatctggatt gggttctgct ccaatagctg cagcagcagc taaaacaaaa gaacctgttg
    12601 aacaagggct tatttcaatg acaggaacct tcattgacac tattgttatt tgtactttga
    12661 cagggattgc aattttagtt acaggaaaat ggctagaatt tgatttacag ggtgctccgt
    12721 taacacaagc atcttttaat accgtatttg gctcactagg tagttttgcc ctaaccttct
    12781 gccttgtctt atttgctttt actactattt taggatggag ctactacgga gagcgttgct
    12841 ttgaatatct attggggact aaatttatta acgcttatcg cattattttt gtcattatgg
    12901 tcggcttagg tggtttctta cagctcgatc tcatttgggt tatcgctgat atcgttaatg
    12961 gtttaatggc tttaccaaat ctaatagctc tcctagcttt gtcaccaatt attgttaaag
    13021 aaacacagaa atatttttca gagactaaaa atatcactaa gccttgaact tactagtttt
    13081 atagtctgct cctaagtgat ataatgtgta gatgaaagga aattaggaaa atggcaactt
    13141 ctactgaaaa cttacaactt gcaaaaagag ggcctattat tagtatcatt gcctatataa
    13201 cactggcagt cgctaaatta gctgcaggtt attggtttga tgctacttca cttgtagcag
    13261 atggatttaa taacttatct gatatcttag gaaatgtagc actcttaatt ggtttacact
    13321 tggctagtca accagccgac tcaaaccatc gttttggtca ctggaaaatt gaagatttag
    13381 caagcctgat tacctctttt atcatgtttg tcgtaggtat tcaagttttt attcaaactg
    13441 tcactaagat tattaataat actgatacta acattgatcc tctaggtgct atagtaggtg
    13501 ctatatctgc tcttgttatg cttggcgttt gtttctataa taaacaatta tctcaaagag
    13561 taaagtctag cgctctggtt gcagcttcta aagataacct ttctgatgct gtgacatcta
    13621 tcggcacatc tattgctatc attgcggctt ctctaaattt tcctattatt gatcgtttag
    13681 ctgctatcat tattacttat tttattctta aaacagctta tgatattttt atagaatctg
    13741 ctttcagctt atcggatggt ttcgatgatt atcaattaaa acaatacgaa aaagctatat
    13801 taacaattcc taaaatatcc gctgtaaaat ctcaacgtgg acgtacttac ggtagtaata
    13861 tttaccttga tattgttctc gaaatgaacc ctgatctctc agtctttgaa agtcatgcca
    13921 ttaccgaaaa agtggaaaaa ctactcagtg ataaattttc tgtatatgac atagatattc
    13981 atgtagaacc cgcttctatc ccagaagacg aaattttcga taatgtttac caaaaattat
    14041 acaaaaacga aaaaatcatc ctagcaaaaa tccccggtta tgagactttt attagccctg
    14101 acttttatat gattaatgaa aagggaaata ttatcacttc tgacatgctt actaatgcta
    14161 caaatcattc attggcaagc aactttaaat atttcaatgt aaaatcaatt agtcaaaaaa
    14221 caaaacttgt gtcttatgaa ttggaaggta aacgacatac tagtatttgg cgccgtaatg
    14281 aaaaatggtt cttaatctac catcaaataa ctgccaagtc aagtccgtat aaaacgagac
    14341 gatatcaaat cacttccctt gattaatata ctatttaatc agttactctt gataaaaata
    14401 gcttacaaaa acaaattaaa aagattgaga agaatctccg tctttttaat ttgtttttat
    14461 gtaaacgttt catagccttg aaaattactt tcagagtagt agactataat ggaaggagtt
    14521 tttcatgaaa ttatacgttc aattaatggt tattttaacc ttttcattcg ctggtgaggt
    14581 tctatctact atattcaact tacctatacc aggtagtatt attggattaa tattactatt
    14641 cctagcgtta aaatacaaaa tcatcaggct caggcatatt gatactgtgg ggaattttct
    14701 attagcaaat atgaccattt tatttttacc accagcagtt ggactgatgg agcactttca
    14761 agacataaag ccctatcttt ttgggatagc tatcattatc ttaggagcgc tatttctcaa
    14821 tattctaact attggactgg tatcccaatg gatcaaaaag agatatgaag gagattatcc
    14881 agaaattgga ggtaaaaatg gcaactttaa cgaataaccc tatctttggt attatgttaa
    14941 ccgtatgggc gtattacatt ggcataagga tttttagaaa atatccttcc cctgcaacaa
    15001 ctccacttct tttggcgact attttattaa ttgctttttt aaaattaaca catatctcct
    15061 ataaagatta ctacaatggt ggttcatttc taacgatgtt aattacgcct tcaacggttg
    15121 tattggctat tcctctctat agaacctttc atttaatgaa gcatcacatt aaaagtatta
    15181 gcattagcat catcttagct tctgttatca acacagtttt taccgcaata gttgctaagt
    15241 tttttggtat gaaatatttc ttagcaatct cacttttccc aaagtccgta actacagcta
    15301 tggccgttgg aattacaagt aaagcaggtg gattagctac tatcactctg gtcgtagttg
    15361 ttattacagg catcttaaca agcgttttgg ggcctatttt tctaaaatta ttaaaaatag
    15421 atgatcctgt tgctataggg ctcgcgctag gaggaactgg gcatgctata ggaactggac
    15481 aagccttaaa atatggccaa gttcaaggcg ccatggctgg actagcaatt ggtatcacag
    15541 gaatttgcta tgttattgtc agtcctttag ttgcaggctt aatattgaaa tgaggaaaag
    15601 aaaagtaaac ccaaaataat tatgttataa tatttaaaaa tagaatttag gataaataat
    15661 tatgataccg ttaaaaaaaa taactgttgg aacgattgtc tgtctctcat ttttgggttt
    15721 aacagcatgc tcttcttcaa atactcagca aacctctact tcaaaatcaa atgtatcaca
    15781 acataaaaat atcaaagctg accacgaaga attacgtttg aaatttaata aagtaaagtt
    15841 aggggttaaa gcaaataact ttaaaggagg cactagtctc gctgaattaa aacaattatt
    15901 tggtggcgaa ccaaatgaaa aatttgatac accagcggga aatgtcactc ttaaaggcta
    15961 tcgttggaat gtcgatgata tctccatcac tatacaatta ttaaacgata gtagcattgt
    16021 tcgttcaatt agtaatttta agtttattcg tgatgccaac atcacaacaa aagattataa
    16081 tagtttaaaa aatggcatgt catacaataa agtaaaagaa cttctaggag aacctgatga
    16141 tatttctcaa gcagtttctt cagataaaga ggaactacag gctgcatgga ttagtggcat
    16201 ccagtcgtca gacagtgacc cgggaattaa ccttacattt gaaaatgata aactcactaa
    16261 caaacaacag catggtttaa aataaaaagg cattaacgcc tttttattaa taccttaatt
    16321 acttattaga aacattttat tacacaatgg tttaatctta gtaccttaac ttagcccctt
    16381 aatggtcata ctaactaatt atgatactaa cttacacaga tttcaaactg attctccctt
    16441 atttgtcaac cctagtttta cttatcagaa atgaggagaa tcagtctgtg aggcaaggtt
    16501 ggagataaac taatataaag cctataacat aaagctttat ggagcatcta ttatccatct
    16561 taagggaatc gaacccctat cccaagaacc ggaatcttgt gtgatatcca ttacactaaa
    16621 gatggtagta catgttacat tatagcatac tttatcaaca aaaacaaaaa gacttgcttt
    16681 agcaagtctt ttaattatta taattcaata tcaccgaata agtcagccat agagaatcca
    16741 gtttgtgttt ctggaagctc atagtcgcgt ttttcttgac gacgtggacg acgtggacgt
    16801 gattgacgtt tttcttcttt ttcaccttct gcttgcgctg gacgctcttc aagagctttc
    16861 attgaaagtg atacacgttc tgcatctgaa ttaacttcaa gaactttaac agtaacttct
    16921 tgtcctgcag ataatacgtc tttaggattt tcaacacgtt tgtgtgaaat ttgtgaaatg
    16981 tgtacaagtc catcaatgcc aggtaacact tcaacaaaag caccaaagtc agtcaagcgt
    17041 tttactttac cttcaataac atcaccagca gcaagttttt gttcaacgcc atcccatggg
    17101 ccaggtgtag ttgctttaag tgaaagtgat acacgaccag cttcttcatc aattgaaagt
    17161 actttaactt caacttcttc accaacagtt acaactgatt taggtgaaac attgcgctca
    17221 tgtgataatt cagttacgtg tacaagtcca tcaacaccac caaggtcaat gaaagcacca
    17281 aagcttgtta aacgagcaac tttaccagtt acaactgaac ctacttcaat gtttgagaaa
    17341 acttctttac gtgcagcagc tgctgattct tcaacaactt cacgacgtga aaggatgaag
    17401 cgattttctg ctgcatcaac ttctttgatt ttagcatcaa attcttgtcc aacaaatttt
    17461 tcagtgttac gtacaaaacg agtatcaatc attgaagctg ggatgaaacc acgaagtcct
    17521 tcaaattcaa ctgaaagtcc gcctttaaca gcacgtgtac ctttaacagt aacaacttca
    17581 ccttcacgac caacaagttt gtcccatgct ttacgagctt ccaagcgttt tttagataca
    17641 aggaaagtta ctgtatcagt atctttacct acaacttgac gaagaacaag tacttcaact
    17701 gtgtcgccag ctttaacaaa atcattgata tcagcatcac gatcgttagt caattcacga
    17761 agtgtcaaga caccttcaac accagtacca tcgataacaa cgtttgcttg accgttatca
    17821 actgttaaaa cttccgcagt gacaacgtca ccaggattca cttcgctaac actgtttagc
    17881 aaatcttcaa attcattcat tctaaaaaat cctccaacaa aagctagccg agctagcttt
    17941 tgataacaat atattttctc aaggtaaccc gcaaggacaa caaatatctc tttgacggtg
    18001 tcaaacaaaa gtttgatacc tttgactggg gtagctggat tcgaaccaac gcatgaggga
    18061 gtcaaagtcc cttgccttac cgcttggcta taccccaaat aatggaaaga gagggattcg
    18121 aacccccgaa cccgaaggag cggatttaca gtccgccacg tttagcctct tcgctatctt
    18181 tccgtaacaa cactgactat tctaccacag atgttgttag cacgcaagct ttttaaataa
    18241 atattagcga ttaagattat attcttcaat aatatttccc atagcttgat ctaaattttt
    18301 atagaatcca ctcacttgct cacctttaat cacagcaaat ttagaatcta gctctgcaat
    18361 ttgacctaca attttttttc caataacgag ttggtatcca tcaaccttag ttccattaac
    18421 tgttacttga gcgtctaaaa tttgaatttc aattttctta tcttttttac tcactattaa
    18481 ctccaatatc tcttttattg ttttttattg tacacaaaat actaaaagct tgcaagagca
    18541 agcttttagt atagacttaa tctactttat aaatccaccc ttctggtgct tcaacatcac
    18601 caaactggat ccctactaac tcatcatata gtttacgtgt cgctggacca acttctgtct
    18661 cactgtagaa gacatggaag tcatctccgt tttgaattcc tccaattggt gaaataactg
    18721 cggctgtacc acatgcgcca gcttctgtga atttatctaa ttcgtcgaca aagacatctc
    18781 cttcaatcgc ttccatacca aacctttctt tagcaaggta aagcaatgaa tatttagtaa
    18841 ttgaaggtaa aattgaagga ctcaaagggg tgataaactg attatcttta gtaattccaa
    18901 agaaattagc tgctccaacc tcttcaattt tagtatgtgt cgctggatca agatagataa
    18961 catctgaaaa ttgtttctca tgagcgtatt tcccaggaag aagactagca gcatagttac
    19021 caccaacttt tgcagctcct gtaccattcg gtgctgctct atcatattct ttggaaacaa
    19081 taaagttagt tggtgttaaa ccacctttaa aataactacc tactggcatt gcaaataccg
    19141 taaaaatata ttcttcagca ggtttaactc caataatatc tcccacacca attaataagg
    19201 gacgaatgta taatgttgcc cctgtaccat acggtggaac aaactcctca ttagcgcgta
    19261 caacagattt aaccgctgca ataaatttgt ctgtcggaac atgtggcatt aaaagacgat
    19321 ctgctgttct ttgaagtcgt tctgcatttt gatctggacg aaataactga atagagccat
    19381 ctttagttct ataagctttc aatccttcaa aagcttgttg cccatagtgg agggcaggag
    19441 aactttctga aatatgtagc gttgcatcat ctgtcaactt gccatcatcc catttcccat
    19501 ctttaaaatg cgaaatgtat cggaatggta attttctgta agcaaaacct aaattatccc
    19561 aatctaaatt tactgtcata aggtacctct ttctttgggt ttctaactat tgtacgctat
    19621 tatcattatt ttttcaatat tatttgtaaa attcagataa attaccaaac tagtaaaaac
    19681 acaaatatgt ttttacttaa ttttaactga gaatacttct tcgtcagaaa tcttatcaga
    19741 tacaaaagag ccattacttg tacgttcaga aaaactccat tgacttaaat caacatcgta
    19801 aacttttcct gttttagaca taatctcaag tacgaaagta tctccttcat gattcgatac
    19861 tgaactaaat aaatcaaatt caaaagatga atcctctaaa tatacaggtc ctgctttaaa
    19921 aacgcgatgc ggtttggatt tcaattcacg aagaacttga agtcctcgat tagcacgact
    19981 agttgtgggg ataacatcaa tggccattcg tttaagactt cctctatgag ttaataaata
    20041 taaacttgtg gtgttagcta taaatgcaga tacgatgtga tcactatctt ttaaattcat
    20101 cgcttttaca cctgcggctt tactaccaac gactggtaca tcatttatac taaatctcaa
    20161 cacataacca ttataagtga caagtataac atcctctaat tggataggag caacagtcac
    20221 aacgtaatca tctacatttt ttaacttagc atatttagct gtttttgact tatatgtccg
    20281 ccatggactg atttcttgac gttcaaaacg ctttatttgc cctaaactag tgacagcaaa
    20341 ataagtttct ttagtaaaat catctactaa ttcagcataa ataatttctt catttgaagc
    20401 aaagttaaca agattttgtg ataaatgttc accaacgtct ttccatcgaa tatcagctaa
    20461 ttcatgaact ggacgataag caagattacc aaggttagta aacatcaata aatgttgggt
    20521 agtctttgca ttgctcacaa atatcaattc gtcatcttct cgcttaccaa gttcatcaac
    20581 ggttgatgca ttgaaagagc gagggctggt gcgttttaca tatccgcctc tagtaacact
    20641 gacgtatgtg tcctcttcga taattaaact tgcagtatcg atttcaatag tttcagctaa
    20701 ttcttgaagt tcagaacgtc tagtattggc aaatttcttt ttgacctcac gtaactcacg
    20761 tttcattacg ttatacatcg tacgttcatc actaataatt gccttgagca ttgtgatttg
    20821 ttgacgaagt tcttcttctt cttctctcaa cgtcacaata tcagtatttg tgagacgata
    20881 taattgcaat gtgacgatag cctctgcttg agcttctgaa aactcatagc tgaccttcaa
    20941 attctctttc gcatcagctt tattctcaga agcacgaatt aatgcaatca cttcatccaa
    21001 aatagaaaga accctaatca gtccctcaac aatatggaga cgtttttcgg ctttttcttt
    21061 atcaaatttt gaacgagcaa taataatttc tcttcggtga gcgatatagc tagttaatat
    21121 tcttgataga cccacctgct taggggtata gtcatcaata gcaaccatat tgaaattata
    21181 attaacttgt aggtctgtat atttaaaaag ataatttaag acaattgttt catcggcttc
    21241 ttttttgagc tcaatagcga tacgaaggcc atctctatca gactcatcac gcacttctgc
    21301 tattcctgga actttattat taacacgaac atcatcgatt cgttttacta aaactgactt
    21361 attaacttca taaggaattt cagtaacaat aatttgtttc ttaccacctt ttaaggtttc
    21421 aatagcagtt cgcgagcgaa ccgctactct ccccttacca gtctcatatg ccttacgaat
    21481 ttcatctttt ccttgaatga tagcgccagt tggaaaatct ggaccaggta gaaattccat
    21541 taatttatct aatttagctt tagggtgatc aatcatgtac acgacagcat caatgacttc
    21601 tgctaaatta tgtggtggaa tatctgtcgc atagcctgca gatattcctg ttgctccgtt
    21661 gactaaaaga ttaggaaagg cagctggtaa aacagttggt tctttctctg tatcatcaaa
    21721 attccacgca aagggtacag tattcttatc aatatcttga agaagataat cagcaatttc
    21781 tgatagacgg gcttccgtat aacgcatagc tgccgcagga tctccatcca tagaaccatt
    21841 atttccgtgc atttcaatca atgtctcacg atttttccaa tcttgagaca tacgaaccat
    21901 cgcgtcatag atagaggaat ccccatgagg atggaaattc cccataacat taccgactga
    21961 ttttgcagat ttgcgaaatc ccttttcaaa agtattacca tctttattca ttgaataaag
    22021 gattcgacgt tgaacaggtt ttaagccatc acgaatatcc ggcaacgccc gttcttgaat
    22081 gatatactta gaatagcgcc caaaacgctc tcccataatg tcctcaaggg acatattttg
    22141 aatattagac atatttttaa ttttctccta gttggagtgg cttagtatta tttttccact
    22201 tatttgaaat gaatgattgg ctctcttgtc taacgcatac aatattactt gtacactata
    22261 aaacatgaat aaccaaggct ctttattaaa atactgtatt ctcttctaaa gtaaacttga
    22321 cattatcttc aatccattgg cgtcttggag gaactttatc acccatcaag acattaactc
    22381 gacgttctgc tcttgctaaa tcttcaattg ttacacgaat taatgtcctt gtctcagggt
    22441 tcatagtggt ttcccacaat tgatcggcat tcatttctcc aagcccttta tatcgttgta
    22501 atagtgagcc tttaccaaat ttttgtcgta attcttctaa ctctatatcc gtccaagcat
    22561 actcaacaat ttctttctta ccttttcctt ttgacatttt atacaaaggt ggcaaagcaa
    22621 tatagacatg tccctcttca acaagaggtc tcatatagcg ataaaagaaa gttaataata
    22681 gtgtctgaat atgagctccg tcagtatcag catcggtcat aatgataact ttatcataat
    22741 taatatcgtc aagattaaaa tcaggaccaa cacctgcacc aatggtatgg atcattgtat
    22801 taatttcttc gttttttata atatcagcca tctttgcctt agctgtattc aatacttttc
    22861 ctcgcaacgg taaaatagct tgaaactttc tatcacgacc ttgcttagca gagccacctg
    22921 cagagtctcc ttcgactaaa tagagttcat ttttcttagc attttttgat tgtgcagggg
    22981 ttaattttcc agaaagtaga cctttatctt ttttcgactt tttcccattc ctactttcat
    23041 cacgtgcttt acgtgcagct tcacgcgcat cacgtgcttt tattgcctta cggattaaat
    23101 tagatgctaa atctccattt tccataagaa agtaagttaa cttttcagaa acaataccat
    23161 caacaattgg tcgtgctaag ggacttccta gtttatcttt agtttgacct tcaaattgca
    23221 agtgctcctc aggtacaaga atagataaaa tggctgatag cccctcacga taatcagaac
    23281 cttcaaggtt tttatctttt tctttaagaa gtcctgtttt acgtgcatag tcattcatag
    23341 actttgtaat agctgatttc aagccagttt catgtgttcc accatcttta gtacgaacat
    23401 tattgacaaa agaaaggata ttatctgaaa aaccatcatt atactgcaaa gcaacttcga
    23461 tatgaaattc ttgttcttca ccttcgaaaa acatgattgg tgtcaacgtt tctttatctt
    23521 catttaaata ctctacaaaa tcttgaacac cgttttcata atgaaattct agatgctctg
    23581 cttcttctga acgattatct gttaaggtta aagtaacatt ttttaagaga aaggcagatt
    23641 cctttaagcg ctctgcaatt gtattaaact taaaatctat cgttgaaaat actgattgat
    23701 ctggcataaa gctgacagaa gttccagatt ttgattttgg tgccgtgcca atttttttca
    23761 gagttgtaac gggcttacca ccattttcaa aacgttgtct gtaaatagct ccgtcgcgaa
    23821 tgatttcaac ttctaaccaa gaagacaaag cattaactac agatgatcca acaccatgga
    23881 gaccacctga tgttttataa ccaccttgtc caaattttcc acctgcatga agcaccgtga
    23941 agatgacttc aacagtaggt tttcccatag catgcattcc agttggcata ccacgtccat
    24001 ggtctgtaac agttatactc ccatctttgt taataatcac atcaatgcga tttccaaaac
    24061 ctgatagagc ttcatcaact gcattatcta caatttccca cactaaatga tgtaaaccag
    24121 ttccatctgt ggaaccgata tacatccctg gacgcttacg aacagcatct aatccttcta
    24181 agacttgaat agcatcgtcg ccataattcg tcactgttat atcttgttta gtcaaagtga
    24241 cctccaaatt t
//
LOCUS       DATRSG010000026        23411 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRSG010000026 DATRSG010000000
VERSION     DATRSG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23411
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.026"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            62..196
                     /gene="rpmH"
                     /locus_tag="VBD89_001025"
     CDS             62..196
                     /gene="rpmH"
                     /locus_tag="VBD89_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO7135564.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            552..1226
                     /locus_tag="VBD89_001026"
     CDS             552..1226
                     /locus_tag="VBD89_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7135565.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            1226..2413
                     /locus_tag="VBD89_001027"
     CDS             1226..2413
                     /locus_tag="VBD89_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7135566.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            2424..2546
                     /locus_tag="VBD89_001028"
     CDS             2424..2546
                     /locus_tag="VBD89_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO7135567.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            2559..4094
                     /gene="dltA"
                     /locus_tag="VBD89_001029"
     CDS             2559..4094
                     /gene="dltA"
                     /locus_tag="VBD89_001029"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO7135568.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            4091..5356
                     /gene="dltB"
                     /locus_tag="VBD89_001030"
     CDS             4091..5356
                     /gene="dltB"
                     /locus_tag="VBD89_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO7135569.1"
                     /translation="MIQEFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            5371..5610
                     /gene="dltC"
                     /locus_tag="VBD89_001031"
     CDS             5371..5610
                     /gene="dltC"
                     /locus_tag="VBD89_001031"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO7135570.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            5603..6865
                     /gene="dltD"
                     /locus_tag="VBD89_001032"
     CDS             5603..6865
                     /gene="dltD"
                     /locus_tag="VBD89_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO7135571.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTERPSDYK"
     gene            7040..7432
                     /locus_tag="VBD89_001033"
     CDS             7040..7432
                     /locus_tag="VBD89_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO7135572.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            7432..8724
                     /locus_tag="VBD89_001034"
     CDS             7432..8724
                     /locus_tag="VBD89_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7135573.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            8715..9107
                     /locus_tag="VBD89_001035"
     CDS             8715..9107
                     /locus_tag="VBD89_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135574.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILGSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            9117..9398
                     /locus_tag="VBD89_001036"
     CDS             9117..9398
                     /locus_tag="VBD89_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135575.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            9580..10362
                     /locus_tag="VBD89_001037"
     CDS             9580..10362
                     /locus_tag="VBD89_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO7135576.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            10349..10909
                     /gene="rnmV"
                     /locus_tag="VBD89_001038"
     CDS             10349..10909
                     /gene="rnmV"
                     /locus_tag="VBD89_001038"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO7135577.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            10928..11419
                     /locus_tag="VBD89_001039"
     CDS             10928..11419
                     /locus_tag="VBD89_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135578.1"
                     /translation="MEYNVTEALTRAGVDLATLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGEPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            11499..12371
                     /gene="rsmA"
                     /locus_tag="VBD89_001040"
     CDS             11499..12371
                     /gene="rsmA"
                     /locus_tag="VBD89_001040"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO7135579.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVCRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            12375..13226
                     /locus_tag="VBD89_001041"
     CDS             12375..13226
                     /locus_tag="VBD89_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7135580.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            13382..14254
                     /gene="rsgA"
                     /locus_tag="VBD89_001042"
     CDS             13382..14254
                     /gene="rsgA"
                     /locus_tag="VBD89_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO7135581.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIRELYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            14261..14923
                     /locus_tag="VBD89_001043"
     CDS             14261..14923
                     /locus_tag="VBD89_001043"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO7135582.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            14916..15548
                     /locus_tag="VBD89_001044"
     CDS             14916..15548
                     /locus_tag="VBD89_001044"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO7135583.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            15550..16824
                     /locus_tag="VBD89_001045"
     CDS             15550..16824
                     /locus_tag="VBD89_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO7135584.1"
                     /translation="MLVIILIIVLASLTVTIISYPKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            16814..17755
                     /locus_tag="VBD89_001046"
     CDS             16814..17755
                     /locus_tag="VBD89_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO7135585.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            17852..18664
                     /gene="purR"
                     /locus_tag="VBD89_001047"
     CDS             17852..18664
                     /gene="purR"
                     /locus_tag="VBD89_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO7135586.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            18883..19296
                     /gene="rpsL"
                     /locus_tag="VBD89_001048"
     CDS             18883..19296
                     /gene="rpsL"
                     /locus_tag="VBD89_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO7135587.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            19318..19788
                     /gene="rpsG"
                     /locus_tag="VBD89_001049"
     CDS             19318..19788
                     /gene="rpsG"
                     /locus_tag="VBD89_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO7135588.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            19943..22021
                     /gene="fusA"
                     /locus_tag="VBD89_001050"
     CDS             19943..22021
                     /gene="fusA"
                     /locus_tag="VBD89_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO7135589.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            22225..23235
                     /gene="gap"
                     /locus_tag="VBD89_001051"
     CDS             22225..23235
                     /gene="gap"
                     /locus_tag="VBD89_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO7135590.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
BASE COUNT         7786 a         3775 c         4716 g         7134 t
ORIGIN      
        1 ttagtaactg atcaaaatag ccggctaaac gaatacgaaa atctatcagg aggtattcaa
       61 cgtgaaacgt acttatcaac caagtaaaat ccgtcgccaa cgtaaacacg gattccgtca
      121 ccgtatgtca actaaaaacg gacgtcgcgt gcttgctagc cgccgtcgta aaggacgtaa
      181 agtgttatca gcttaatata gatagcaaaa taatatcaag ccaacaggta ctcgagtctg
      241 gtggcttttt tggtaccttc ataacgataa aggtttttgt gagagtagtt tgagtatatt
      301 atagataaag ttaatgaaaa gtgagtttat aatgcggaaa ttcgtagaca aatagcccct
      361 atttttgtta gaatatgagg agatttctta ttgagaaata tctttatagc gagtgatatt
      421 ttatttttta gacgcttgta ttattgaagt agtttagtga cttaggaaat acttttatta
      481 atatcgtttt ttcaaggtta tttcaatttt gctagtttat actataatat ataaggtaag
      541 gtgactagaa aatgagactt cttgtagttg aggatgaaaa aagtatagca gaggctattc
      601 aggctttatt ggcagataaa ggttattctg ttgatttagc atttgatgga gatgatgggt
      661 tagaatacat tttgacaggt ctctatgatt tagtcctgtt ggatattatg ttacccaaac
      721 gctcaggatt atctgtttta aaaagagtga gggaagctgg tttagaaacc ccaataatct
      781 ttttaactgc aaaatcacag acttatgata aagttaatgg tttagattta ggggcagatg
      841 attatatcac aaaacccttt gaagcagatg aattgctagc tagaattagg ctgagaacca
      901 gacaatcttc tctaatcaga gcaaatcaat tgcggcttgg aaatataagg cttaacacag
      961 attcacatga gctagaatct aaagaaagta gtgtaaaatt atccaataaa gaatttctct
     1021 tgatggaagt ttttatgcga aatgcaaagc agattattcc taaaaatcag ttgatttcta
     1081 aggtttgggg accttcagac aattcagaat ataaccagtt agaagtattt atttcttttt
     1141 tgcgaaaaaa attgcgcttt ttaaaagcag acattgaaat aataaccact aagggctttg
     1201 gatattcctt ggaggagagg acataatgtt tagtgattta cgcaaaaaat ttgtcttttt
     1261 gaccatgtca atccttattg tcgttgttct ctttctattt gcagttagta atcgctacaa
     1321 tcaatattgg gatgaatatg atgcatatag gattgttaaa ttagttgcca aaaatgatta
     1381 tttaggaata ccaggcgatg aaccgattgc tttggtgact atagataacc aaaagatggt
     1441 taagattcaa agcaataata ctgatttgac aaatgatgtt atcgaaaaat ctagtttgaa
     1501 gctccttgag caaggaaaga agtcacgaaa atggaagtct tttatatatt ccataaaaga
     1561 atataaagat aaaacctaca ccatagctat tatggatttg gctagttatg aagtccctta
     1621 tgcaaggcgt tttctaattc ttgtttttac tatttttgga ttttgtttat tagcagctgt
     1681 aagtctttat ctttctcgat ttattgtagg acctgtagag acagaaatga ctcgcgaaaa
     1741 acagttcgtt tcagatgcga gtcatgagct gaagacccct attgcagcta tacgagccaa
     1801 cgttcaggtt ctggaacagc aaattccagg gaatcgttat ctggatcatg ttgtttcgga
     1861 gactaagcga atggagtttt taattgaaga tttgcttaat ttgtctagac ttgatgaaaa
     1921 acgctccaag gtaaatttca aaaagttaaa cttatctgtc ttatgtcaag aagtacttct
     1981 aacatatgaa agtttggcat atgaagaaga aaagtgttta aatgatacga ttgaagatga
     2041 tgtctggatt gttggagaag agagtcagat taagcaaatt ttaataattc ttttggataa
     2101 tgctattagg cattctttgt caaaatcagc aatccagttt agtcttaaac aagctcgaag
     2161 aaaagctatt ctgacaatat caaatccaag tgctatctat tccaaagagg ttatggataa
     2221 tctctttgag cgtttttatc aagctaaaga tgatcatgca gattctctca gttttggtct
     2281 aggcttatct attgctaaag caatcgtgga acgccataag gggagaatta gagcatatca
     2341 agaaaaagac caattacgtc ttgaagtcca gttaccaatt gatggatttt ggaccaatac
     2401 catgattaat taaaggaaga atgatgaaac catatttatc ttttattggt agaacgttat
     2461 tatacttcgg tattttattg ttactaattt acttttttgc ataccttggt cgcggacaag
     2521 gcagttttat ttataatgaa ttctaggaga tggtacaaat gatacatgat atgattaaaa
     2581 caattgagca ttttgctgag acacaagctg attttccagt gtatgatatt ttaggggaag
     2641 tccatactta tggacaactt aaagtagact ctgactctct agctgctcat attgatagcc
     2701 taggccttgt tgaaaaatca cctgtcttag tattcggtgg tcaagaatat gaaatgttgg
     2761 cgacatttgt tgctttaaca aagtcagggc atgcttatat accggttgac caacactctg
     2821 ctttggatag aatacaggct attatgacag ttgctcaacc aagccttatc atttcaattg
     2881 gtgaatttcc tcttgaagtt gataatgtcc caatcctaga cgtttctcaa gtttcagcta
     2941 tttttgaaga aaagactcct tatgaggtaa cacattctgt taaaggtgat gataattact
     3001 atattatttt cacttcaggg actactggtt taccaaaagg tgtgcaaatt tcacatgaca
     3061 atttattgag ctttacaaat tggatgattt ctgatgatga gttttcagtt cctgaaagac
     3121 cgcaaatgtt ggctcaaccg ccatattcat ttgacttatc agttatgtat tgggcaccaa
     3181 cactagctat gggaggcacc ctgtttgccc taccaaaaac agtagttaat gatttcaaaa
     3241 aactattcgc taccattaat gaattgccaa tacaggtttg gacttcgaca ccatcatttg
     3301 ctgatatggc gctactatct aacgatttca attcagagac cttgccacag ttaacacatt
     3361 tttattttga tggggaagag ttaactgtca agactgcaca aaaacttcgt caacgttttc
     3421 caaaagctcg tatcgttaat gcatatgggc caacagaagc aacagttgct ctatccgcag
     3481 tagcaattac tgatgaaatg ttagaaacat gcaaacgcct tccaattggt tacactaaag
     3541 atgactcacc aacgtatgtg attgatgaag aaggtcataa attaccaaac ggagagcaag
     3601 gtgaaatcat tattgctgga ccagcagtat caaaaggcta tcttaacaat ccagaaaaga
     3661 cagctgaggc atttttccaa ttcgaaggtc tacctgctta ccataccggt gacttaggaa
     3721 gtatgaccga tgaaggtctt ctgctttacg gtgggcgtat ggatttccaa attaaattta
     3781 acggctatcg tattgaatta gaagatgttt ctcaaaactt aaacaaatcg cagtatgtaa
     3841 aatcagcagt agcagtgcca cgttataaca aggatcataa agttcaaaac ttattagcct
     3901 atattgtctt aaaagaaggt gtaagagatg attttgaacg tgatttggat ttgacaaaag
     3961 caattaagga agacttaaag gacattatga tggattacat gatgccatct aaatttatct
     4021 atcgagagga tttacctttg acaccaaatg ggaaaattga tatcaaaggt cttatgagcg
     4081 aggtaaacaa gtgatacagg aatttttaga aaaacttcct cacttggatg tttatgggaa
     4141 tccacaatat ttcttctacc ttatcttagc tgtcctacca atttacatcg gcttattctt
     4201 taagaagcgt tttgccttat atgagattat ttttagtcta agttttattg taatgatgtt
     4261 gactggtagt acttttaatc aattgaagtc actattggca tacgttgtcg gacagtctct
     4321 gctagttttt atctataaag cttaccggaa acgatttaat catactttgg tcttttatgt
     4381 aacggtttgt ttatctattt ttccgctatt tttggtaaaa ttaattccag ctatatctga
     4441 ggatgggcat cagtcacttt ttgggtttct aggaatttct taccttactt ttagagctgt
     4501 tgctatgatt attgaaatga gagacggtgt cttgaaagaa tttactttat gggaattctt
     4561 aagattttta ctcttctttc caactttctc aagtggacca attgatcgtt ttaaacgatt
     4621 caatgaggat tacattaata tcccagatcg aaacgaactc ctagatatgt taggtcaagc
     4681 gattcattat ttgatgttag gttttctcta taagtttatt ttagcctata tttttggaag
     4741 tctgattatg cctcctctaa aagaattagc gctagaacag ggtggtgtgt ttaattggcc
     4801 aacacttggg gttatgtatg cctttggttt tgatttgttc tttgattttg caggttacac
     4861 aatgtttgcg ttggctattt ctaacctaat ggggattaag tctccgatta actttgacaa
     4921 acctttcaaa tcacgcgacc taaaagaatt ttggaataga tggcatatga gcctttcttt
     4981 ctggtttaga gactttgttt tcatgaggct tgttaagctt ttagttaaaa ataaagtttt
     5041 taaaaaccgt aatgttactt caagtgtagc ttatattatc aatatgcttc ttatgggatt
     5101 ctggcatggg ttaacttggt actatatagc ctatggtctc tttcatggga ttggcctagt
     5161 tattaatgac gcttgggtac gtaagaagaa aaatattaat aaagaaagaa gattggctaa
     5221 aaaaccactt ttaccagaaa acaaatggac ttatgctttg ggtgtcttca tcacctttaa
     5281 tgtagttatg ttttctttct tgattttttc aggattttta gatcttttgt ggttcccaca
     5341 accgcataac aaataataag gagtattata atggatatta aatcagaagt attagcaatt
     5401 attgatgacc tatttatgga ggatgtttct tctatgatgg atgaagactt atttgacgca
     5461 ggagttctag acagcatggg tactgttgaa ctaattgttg agttggaaag ccactttaat
     5521 attgatattc cgattgcgga atttggtcgt aatgactgga acacagctaa taaaattgtt
     5581 gctggagtaa cggagctctg caatgcttaa acgtctaggt aaagtatttg gacctctagt
     5641 ttgtgcttta ctattgttgg taggattata ttttgttttt cctgtttctc agcctcatca
     5701 tttaggtaag gaaaaaaaca gtgcagtagc gttgacgaag gcaggtttta aaagcagagt
     5761 tcaaaaagtt agagctttca gtgatcctaa agccaatttt gtccctttct ttggttcaag
     5821 tgagtggcta agatttgacg caatgcatcc atcagtttta gcagaggctt acaataggtc
     5881 ttatatccca tatcttttag gtcaaaaagg ggcggcttct ctgacacaat actatggcat
     5941 tcaacagatt aaaggacaaa tcaaaaataa aaaagctatc tatgttattt ctccgcaatg
     6001 gtttgttcgc aagggagcca acaaaggtgc ttttcaaaac tatttcagca acgatcaaac
     6061 cattcgattt ttgcaaaatc aaacagggac aacctacgat aggtatgctg ctcgtcgatt
     6121 gttaaaatta tatcctgaag cttctatgtc agatttgata gaaaaagttg cagatggcca
     6181 aaaactatca aataaagaca aacaaagact aaagtttaat gattgggtat ttgagaagac
     6241 agatgctatt tttagctatc taccactagg aaaaacttat aatcaggcaa taatgcctca
     6301 tgttggtaaa ttaccgaaag cattctcata taatcattta tcgcgtattg catcacaaga
     6361 tgctaaagta gcaacgagat caaatcaatt tggtattgat gatcgctttt accaaacaag
     6421 aattaaaaag cacttaaaaa aattgaaagg gtcacaacga catttcaatt atactaagtc
     6481 accagaattt aatgatttac agttggttct taatgaattc tcaaaacaaa atacagatgt
     6541 cctttttgtc ataccaccag taaataaaaa gtggacagac tacacagggc ttgatcaaaa
     6601 aatgtatcaa aaatctgtag aaaaaataaa acaccaactt caaagtcaag gtttcaatca
     6661 tatcgctgac ctttctcgag atggaggtaa gccatacttt atgcaagata caatccattt
     6721 aggttggaat ggttggttag agctagataa gcatatcaat ccatttttaa cagaggaaaa
     6781 cagcaagccg aattatcaca ttaataataa atttttgaag aaatcttggg caaaatatac
     6841 agaacgtcca agtgattaca agtaaatagt agagtcagat gatttatagc attagagcta
     6901 ttaatcatct tttttaatat ctttgtattt ggtaatccta agataactaa tccatgtttt
     6961 atagtttttc atttataatg agaataaatt tatgataaac tagatatcaa atgagtgtta
     7021 tatgataaaa ggaggagaga tgaagtcaat aaaattactt agttttattg taataatcct
     7081 tttagggctt ggtttcactg taggaggttt aaaagtgact gaaaacaata aacaacaaga
     7141 attatgggaa atagctaata gcaaagacgc taaaaatgct tatcaaaagt ggatttatgc
     7201 agaagatgaa gatgctttca aagagaatgc tgttattaag tcgtatgata ttgacaaaga
     7261 aagtatcaaa aaaaatccta tgggagggat ttcggtaagg ttaatcatta ataaagatcc
     7321 taacttatat attacttgta atttggatag ggataatcaa gggcatttgg tcagtcaaag
     7381 tagccatcaa tctccccagt taactcattt attggaaagt agaggtcatt aatggaaaaa
     7441 gtcttgactg atttagatag ggtaaatata gcgaagcaag agtatgaatt aggtagccaa
     7501 cttgatacac tagtcaaaat aatgagccaa gataaggtac tccccatcgg taaagttgct
     7561 cacgttcaag atggaggcaa agaaaccggg gaacaaattt ataccatcac tccaaatgga
     7621 actttagaca agccagagga tgttaaggaa gttacggttt tatttaaggg gtcaacagca
     7681 ccgtttggag gggatgattg gaaaacagac tggtttaaaa atgatattcc aatagctagt
     7741 aaactattat taaagaaatt tggttcacaa agtgtatctc ataaacaagg tacaaagcag
     7801 ttagaacaat cagctcatct gctaaaagaa gttatgaata aatatcctaa cgcaaaaatt
     7861 tcggtctacg gacactcgtt agggtcaatg aatgcacagt atgctattgc agaattggat
     7921 gataatcaaa tttaccgagt tcaaggagct tggatatata acggtccaaa cctttatagt
     7981 attttgacag ataaacaaaa agaacaggtt gataaaataa aaggggtggt caataattac
     8041 gttgattcca aggataaaat tggaataggt tatcctaaag aaggaagcga taatgctgtc
     8101 ggaattgtta aaatagttga cagtaaagct gtttcaggag gtagctgggt tgctaatttt
     8161 accaatcagc atatttgggg aggctaccag tacaatccag atggttcttt aaaattagca
     8221 gaagataaat ttgcttacaa agaaaactat tcagtagttc atacgaatgt gaatcaaaga
     8281 atgcatgcta ttaaagcgaa acttagtagc tttaaaagta cgggaaaaga agttagtaag
     8341 aatcaagcta ttttcctaga tagtcaacaa gcaaaagtga tctctagtgg tttggtaacg
     8401 atatcaaaag aagcagttga agaagttaaa aaagttgagc aaaaagcagt tgctaaagca
     8461 gaagagcttt atcaatcttt aaaagtagct cctacaggag ttactgaact aagtcctcaa
     8521 gaattggaag aagcgtatgc tcagtcaggt gttagctatg cgagcattgt cggagaagct
     8581 caaacctttt tctcaaataa aactcaagcg gcagaaagca tttctcacgc ttacgttgaa
     8641 ttagaaaacc aaattaataa aggagttgaa gctatgttgc gacaagatag taacttgtca
     8701 ggggagttta aacaatggca ctgattgata tcattgaaaa acaattagct gatactcaga
     8761 gaaaaatatc agatttagat gatgcctatc accatagttg ttgccagttt gaagaaaaat
     8821 tagacgatct ctcagtaaga aaaaataaga ttattaatat gcttcaagaa acttacgacg
     8881 ctgttgaata tgatttgaga tatagtaatg atagctcaga tatgatgacg ctcaatcgta
     8941 ttttaggttc ttatcatgac gacttagagc aagcttacca taaagagtac tatgccttgt
     9001 ccgctcaaga agaagaatat agagctaact atattcggca acgttcagaa catgaactaa
     9061 cctttgaaga gttgcaaagg gaaaagaaaa gagaattaat gaagtaggag aaattattga
     9121 gtttaatgtg ggatgtcctg actggtaagg agtcagtaag gactttaaaa aaacaattgg
     9181 ataaagaatt gatacatagc gtgtacgcgc cacgcaagag atctaagttt cacgatatta
     9241 ttacgtcgca tcataaagaa atagacgaag tagccatcaa cacaaagaca gctattcata
     9301 aagttgctaa tcaattggac gaggcaataa aaggagaatt tcgttcaaag gttgaggagg
     9361 ctattgaaag caatcagcaa aaatataatc atttatagtc tagtttctct accattagct
     9421 agacacttgt agaggatttt tacagcaagt attcaaaagt cagagcaaaa atgttttgac
     9481 ttttttgatt tactctttct gatcagaatg cttcagtctt atgttataat aataaagatt
     9541 aatgcttgta gctacgataa gcaaagaaag aacgatgata tgataaaaat atttgatacc
     9601 catacccacc ttaacgtaga aaattttgaa ggaaagatag acgaggaaat taatttagct
     9661 agcgaactcg gtgttacaaa aatgaatgtt gtcggctttg accaagatac catttcaaaa
     9721 tctttggaat tatcttccca atatgcacag gtatatagca caataggatg gcatccaact
     9781 gaagctggtt cttatgatga taatattgag tcaatgatta tcagtcattt agaaaatccc
     9841 aaggttattg cacttggtga aattggtctg gattattatt ggatggagga tcccaaagat
     9901 attcagatcg aggtgtttaa gcgtcaaatt gaactttcaa aagagtataa ccttcctttt
     9961 gtcgttcata cacgtgatgc gttagaagac acttatgaag tcattaagga atctggagtt
    10021 gggccttttg gtggtataat gcactctttt tctggttctt tggaaatggc tcagaaattt
    10081 attgatttag ggatgatgat ttccttttct ggtgttgtga cctttaagaa ggcattagat
    10141 gtacaagaag ctgcacgaga gttgccatta gataagatat tagtggaaac agatgctcct
    10201 tatttggctc ctgtacctaa aagaggtcga gaaaacaaaa ccgcttatac gagatatgtc
    10261 gttgaaaaaa tagcagagct acgaggcata actgtggaag aggtagcgga ggctacatac
    10321 caaaatgcag taaggatatt tagacttgat gaaaaaaatt gatattcaag aagtgatcgt
    10381 tgtcgaaggg aaagatgata ctgctaatct gcgacgcttt tacaatgttg atacctatga
    10441 aactagaggt tcagcaattg atgaagatga tttagagcgt atcgaacgtt tgcacaattt
    10501 gcggggagtt atcgtcttta cagacccaga ttataatggc gagcgtatcc gtaaaatcat
    10561 catgaatgcc attccgacag tccgccatgc cttcctcaat cgagacgagg caaagccagg
    10621 ctcaaagacc aagggacgat ctctgggtgt cgagcatgcc agctttgaag atttgcagaa
    10681 ggcactctca aaagtgactc agcatttcga tgatgaagat catttcgata tcacccaagc
    10741 agacctcatt cgttggggat tcattactgc tagcgatagc cgaaaacgcc gtgagtatct
    10801 tgggaatcaa ctccgtatcg gctactcaaa tggtaaacaa ctcctcaaac gcctcagact
    10861 cttcggagtg accaaggcag aagttgaaga gtgtatggag gggtattaat agaaaaggag
    10921 aaaaatagtg gagtataatg taacggaagc attgactaga gctggggtag atttagcaac
    10981 gcttgcagtc aaagggactg caactatggt aaccagtaaa attcaatcaa taaaggatgt
    11041 aaaagatgct gcaacattga gagctactta tgatgattta attaacgaat tattagctga
    11101 acgggagcaa gcaattcgca ttgcacagtc atataaagaa gagtatgaaa aaattaatat
    11161 agatgatgag gatattgaat atctatccaa tactctcgag caaggaatct cattgttata
    11221 taatttttct ggtctaaatg aggaacagga acgaacaatg aaacaattgg ttacattatt
    11281 gaataaagat actttaaaaa caatgcagtt gattggattt aattataagc aagcaatagg
    11341 agaaccacta accgaagctt gtgcaactgc aattaaaaat aagtttataa ctcagaatag
    11401 taaaggcaaa aggaagtagt ggtataatcc agttgtaata tcacaaatag cctcaaatgt
    11461 tatttcaaaa atgatcaata ttaagaaata gaggaattat gagaatcgca gataaaaccg
    11521 ttacccgcgc tattttggag cgtcatgggt ttacatttaa gaaatcattc ggtcagaact
    11581 ttttgaccga tactaatatt ttacagaaga ttgttgacac ggcagagatt gacaagggtg
    11641 tcaacgtgat tgagattggt cctggtatcg gggctttgac agagtttttg gctgagaatg
    11701 cagctgaggt tatggctttt gagattgatg atcgtctcat tcctatcctt gctgatacgc
    11761 ttgcgcgttt tgacaatgtc caagtggtca atcaggatat cctcaaggct gatttgcaga
    11821 cccagattca ggctttcaag aatcctgatc tgcctatcaa ggtggtcgca aacctacctt
    11881 actacatcac aacaccaatc cttatgcact tgattgagag caagattcct tttgcggagt
    11941 ttgtggtcat gatacaaaaa gaagtggcag atcgtatttc agctatgcca aatactaaag
    12001 catacggttc tttgtcaatc gcagtgcagt attatatgac tgccaaagta tcatttatcg
    12061 ttccgagaac tgtttttgtt cctgctccaa atgtagattc agctattttg aaaatggtct
    12121 gtagagatca accagtagtg agtgttcaag atgaggattt ctttttcaga gtgtcaaaag
    12181 tagccttcgt acatcgtcgt aaaacacttt ggaataatct aacgagtcat tttggaaaat
    12241 cagaagatac taaagcaaaa ttggaaaaag cattagaaat agcaaaaatt aagccatcta
    12301 ttcgcggaga agccttatct atccctgatt ttgcaagttt agcagatgcc ttgaaagagg
    12361 ttgggatata aactatgttg gttgacaaga agtggcgttt tgaagatagt gcaagttatt
    12421 tcgcttgtcc taaatgtcaa aatcccttga taaaagaaag taacagcctc aaatgctctg
    12481 ataatcattg ttttgattta tccaaatttg gatatgttaa tcttcttgga ggaaagaagg
    12541 tagatgaaca ttatgataaa aaatcatttg aaaatcggca gcttgtctta gaaaacgggt
    12601 attataatca tattttagaa gctatttcaa aggttttgga aaataattct cagtttcatt
    12661 cggttttaga tattggttgt ggagagggct tttattctcg tcaattagtt aacaagcatg
    12721 agaagacttt cttggctttt gatatttcta aagattcgat tcaattagct gctaaaagtg
    12781 atcagtctag gcttgtgaaa tggtttgttt ctgatttagc taaccttcct attcaagact
    12841 catcaattga tatcatttta gacatttttt ctcctgcaaa ttataaagaa tttaggcggg
    12901 tgctgagtga tgatggtatc cttgtaaagg ttgttcctgt agctgaacat gttcaagaat
    12961 tgcgagaaaa agctagtcaa tacttgaaac agaaagatta ctctaatcaa aaaattctag
    13021 atcactttag agaaaatttt gaaattattt ctgagcagaa agttgttcaa agctataact
    13081 gttctcaaca agaacgtcaa gcttttattg atatgacacc gttgttattc agcgtagata
    13141 aaacgactat tgattgggcg agtatatcag aaattacagt aggtgcctta atagtaattg
    13201 gaaaaaagcg cagtgtatct aaataagatc actgctttta tgttgggaat tcataaaatt
    13261 gaggttattt gagagaaatc gaacacctgc aaatagtttc tatttagctt atctctgaga
    13321 gtagagctta tttctcattt tatgatataa tagtggtatt ctaaacaatt aaggagaaca
    13381 attgcaaggt agaattgtca agtcgctagc aggtttttat tatgtggaat ctgacggtgt
    13441 tgtttatcag acacgtgcgc gtggaaattt tagaaaaaaa ggtcaaattc cctacgttgg
    13501 ggattgggtt gaattttctt cgcaagatca atcagaagga tatattctat caattgaaga
    13561 gcgtaaaaat agcctcgtac gaccacctat tgttaatatt gatcaagctg tcgttatcat
    13621 gtctgctaaa gaacctgatt ttaacgctaa cttattagac cggttcttag tacttctaga
    13681 atataagatg attcagccta tcatttatat ttccaagttg gatttgttag acgatttggt
    13741 tgttattgat gatattcgcg aactttatca gaatataggc tacgttttct gttattctca
    13801 agaggaactc ttgcctctgt tagcaaataa agtgactgtt tttatgggcc agacaggtgt
    13861 cggaaaatca acgcttctca acaaaattgc tcctgagttg aaacttgaaa ctggtgaaat
    13921 ttcgggtagt ttaggccgtg gccgacacac cacaagagca gttagtttct ataacgttca
    13981 taaaggaaaa attgcagaca ctcctggttt ttcttctttg gactatgaag tggataatgc
    14041 agaggactta aacgaatcct ttccagagct tcgtagactt agtcattttt gtaaattccg
    14101 ctcgtgtacg catacacatg agcctaaatg tgctgtaaaa gaagccttaa ctcaaggtca
    14161 gttatggcaa gtccgctatg ataactacct ccaattttta agtgaaattg agaaccgtcg
    14221 tgaaacatat aagaaagtta ttaaaagaaa gtaggtttct atgtcaacca ataaaattgc
    14281 cccatcgata ttagcagcag actatgctaa ttttgctaac gaattgaaac gtattgaaga
    14341 gacaacagca gaatacgttc atattgatat catggatggt caatttgtcc ctaatattag
    14401 ttttggtgcg gatgttgtat ccagcatgcg taaacacagt aaacttgttt ttgattgtca
    14461 tctgatggtt gttgatccag aacgttatat tgaggctttt gctcaagctg gtgctgatat
    14521 catgaccatt catgttgaag caacgaaaca tatacatggt gcgcttcaaa agataaaaga
    14581 ggcaggaatg aaagcaggtg tggttattaa cccaggaaca cccgtagaat ctcttattcc
    14641 tatcttagat ttggttgacc agattttaat tatgactgtt aatcctggct ttggtggaca
    14701 agcctttatt ccagaaatga tgtcaaaagt aaagacagtt gcagcatggc gcaaagagta
    14761 cggtcatcat tatgatattg aagttgatgg cggtatagat aatactacaa ttaaggcagc
    14821 agcagaggct ggtgcaaatg tctttgttgc aggttcttat ctctttaaag caagtgattt
    14881 acctgcacaa gtagaaacct taagggtggc gctcgatgac taaaattgct ttatttgctg
    14941 gcggtgactt aacttatttt gaatatgatt ttgactattt tgtcggaatt gaccgtggaa
    15001 gcctattttt attaaaaaat gggctttctt tggacatggc agtgggtgat tttgattcta
    15061 taacagaaga tgaactgctt tatatcaaac actattgtag caatatcgtt tctgctagtg
    15121 cagaaaaaaa tgatacagat acagaattag ctttaaaaac tattttcaaa gaatttccag
    15181 aagctcaggt gaccgtattt ggagcatttg gtggcaggat tgaccatatg atgtctaata
    15241 tctttttacc aagtgacaga gatctagaac catttatgtc acaaatcaga ctcaaagatg
    15301 aacaaaacat agtgacttat ctaccatccg ggaaaaatca agtctcaagg attgaaggga
    15361 tgtcctatgt ctcgttcatg cctgagagtg agtctacttt gcagatttct ggggctaaat
    15421 atgaattgaa caaaagtaat tattttaaga aaaaaatgta ttccagtaat gaatttatga
    15481 cttcgccaat tgaagtagag ctcaaagatg gttacttaat cattatttat agtaaggata
    15541 gaggttaata tgcttgtcat cattttgatc attgtactag ctagtctgac agtgacgata
    15601 atttcttacc caaaaatgac ggaattaaca aagtccgttg aaaaacaact tgaagataat
    15661 gctgataatc tatcagacca actgacatat cagatagaag tggcgcaaaa agatcaaatt
    15721 ctaacgttga ctaatcagct aaaccgtatg caacaggaaa tttatcagtt gcttaccgat
    15781 atgcgtacag aattaaatca gcatttaacg gagagtcgag atcgatcaga caaacgcttg
    15841 gaattgatta actccaattt atctcagtca gttcagaaaa tgcaagattc caatgaaaaa
    15901 cgcttggatc aaatgcgcca aactgttgag gaaaagctag aaaaaacgct acaaacgcgt
    15961 ttgcaaactt cttttgaaac tgtatcgcgt caactagaga gcgtcaatca aggtcttggg
    16021 gagatgaaaa ctgttgccca agatgttggt acactgaaca aagtcttgtc aaatactaag
    16081 acaaggggaa tattagggga gttacaactc ggccaaatta tagaagatat tatgacagtt
    16141 agtcaatatg agagagaatt tcctacggtg tctggctctt ctgagcgtgt tgaatatgct
    16201 attaaattac ctggaaatgg tcagggagat tatatctatt tgcctattga ctctaagttt
    16261 cctctagaag attattaccg attggaagat gcttatgaat tgggtgacaa ggttcaaata
    16321 gaactctatc gtaaatcttt actggcaagt attcgtaaat ttgcaaaaga tataaacaat
    16381 aagtacttaa atcctcctga aacgacaaat tttggtatca tgttcttacc aactgaaggg
    16441 ctctattctg aagtggtaag aaatgcaaca ttctttgata gtctaagacg tgacgaaaat
    16501 attgtagtag ctggaccgtc aaccttatct gctttactaa attcattatc tgttggcttt
    16561 aaaaccttaa atattcaaaa gaatgctaat gatattagta aaattttagg gaatgtcaag
    16621 gttgaatttg gaaaatttgg tggcatgtta tcaaaagctc aaaaacaatt aaatacagca
    16681 agtaaaagta ttgatagttt actcacaacg cggacaaacg ctattatcag agtcttaaat
    16741 acagttgaag aacatcaaga tcaagcaacc actagcttgt taaaccttcc tataacagaa
    16801 gaagaggaaa ttaatgaaaa ttaaccaaat gaaaaaagac gagctttttg aggggtttta
    16861 ccttataaag aaagcagaag ttcgtaaaac gcgtgcaggt aaagacttta ttgcctttac
    16921 ctttcaagat gatactggcg aaatttcagg aaatatgtgg gatgcacaga cctataatgt
    16981 agaagagttt gtagccggta aaattgttca tatgaaaggg cgtcgtgaag tttataatgg
    17041 aactccccaa gtcaaccaaa ttacattaag aaacatcaaa gatggtgaac ctaatgatcc
    17101 tagagatttt aaagaaaaac ctccaatcaa tgttgacaac gtgagagagt acatggaaca
    17161 aatgctcttt aaaattgaaa atgcaacctg gcagcgtgtg gtaagagcac tttatcgtaa
    17221 atacaataag gaatttttta cctatccagc cgccaaaaca aaccaccacg cttttgaatc
    17281 aggattggca tatcacacgg caacaatggt tcgtttggca gatagtatcg gagatatcta
    17341 tccagaactt aataaaagtt tgatgtttgc tggtattatg ctacatgatt tagccaaggt
    17401 cattgagtta tcgggtcctg ataatacaga atatactatt cgaggtaatc ttatcggtca
    17461 tatttcactt attgatgagg aattaacaaa aatactagca gagcttaata ttgatgatac
    17521 aaaagaagag gttacagtgt tacgccatgt tattctgagt catcatggac aattagaata
    17581 tggaagcccg gttcgtccta gaatcatgga ggcagaaatt atccatatga tcgataacat
    17641 tgatgcaaat atgatgatga tgacaacagc tttaaaccgt gtcaacgaag gtgaaatgac
    17701 aaatcgcatt tttgccatgg ataaccgttc tttttataaa ccgaacatta aataatttag
    17761 gtagaataat taagacctaa aataaaatgt tcgtttttag catttgttat gttatactat
    17821 atgtaaagaa tgctatgaat aaggagaaaa tatgaaatta agacgtagcg aacgcatggt
    17881 tgttatttca aattacctaa taaataatcc ctatacatta actagtttaa atacatttgc
    17941 tagtaaatac ggtgccgcga aatcttcgat ttcagaagat attgctatta tcaaaaaagc
    18001 ttttgaacaa gctcaaatcg gagatattaa aactgtgact ggtgcaagtg gaggtgttat
    18061 ctttacacca actattgctg aagcagaagc gaaagaaatc gttgaagaat taagacagcg
    18121 tttatctgaa aacgatcgta ttcttccggg aggttacatt tatctttcag atctactcag
    18181 tacaccaaaa atgttacaaa gtattggtcg cattatagcg aacgcttaca gaggtcaaaa
    18241 aattgatgct gtcatgacgg ttgcaacaaa aggtgttcct ttagcaaacg cagtagcaaa
    18301 tgttttagat gttccttttg ttattgtgag acgtgatttg aagattacgg aaggttctac
    18361 tgtgtcagta aactacgcta gtggttcaag tggccgtatt gaaaaaatgt ttctttctaa
    18421 gcgtagctta aaacctaaca gtcgagtttt aatagttgat gatttcttga aaggtggagg
    18481 tactgtttcg gggatgatta gtttactctc agaattcgat agcacattag taggtgtcgc
    18541 tgtatttgca gaaaatgctc aggaacaacg tgagaagatg gcctataaat ctcttctgag
    18601 agtatctgaa attgatgtaa aaaataatag agtctctgta gaagcgggga atatatttga
    18661 ttaaagagtc aaggtgacat ccttgattct ttttgcttgt aaaagtaata aaagcttgac
    18721 acattatgtg taaaatgata ctatgataaa cggtactttt acttttggtc tctcaaaagt
    18781 gtacagagac gtgctgacaa ttgttgcaaa agtacacgca gatataggct gtcaccaagt
    18841 gctatatcaa ccaaaaataa aaaacttaca ggagaaatgt agatgcctac aattaaccag
    18901 ttggtacgta aaccacgtaa atctaaagtt gaaaaatcag attcacctgc tttgaatatt
    18961 ggttacaaca gtcacagaaa agttcacact aaattatctg caccacaaaa acgtggtgtt
    19021 gcaactcgtg ttggaactat gacacctaaa aaacctaact ctgcccttcg taaatttgct
    19081 cgtgtacgtt tgagcaacct tattgaagtt actgcttaca ttccaggtat cggacacaac
    19141 ttgcaagaac acagtgttgt gcttatccgt ggtggacgtg taaaagacct tccaggggta
    19201 cgttaccata tcgttcgtgg tgcacttgat actgcaggtg tagctgatcg taaacaaggc
    19261 cgttctaaat acggtgctaa acgtcctaaa gggtaattag aagggggata agagaaaatg
    19321 agtcgtaaaa atcaagcgcc taaacgcgaa gtattgccag atccattgta taactcaaaa
    19381 atcgttacac gtcttatcaa ccgtgtaatg cttgatggta aacgtggtac tgctgcaact
    19441 atcgtatatg atgcttttga agcaattaaa gaagctactg gaacagatgc tcttgaagtg
    19501 ttcgaaacag ctatggaaaa catcatgcct gtacttgagg tacgtgcacg ccgtgttggt
    19561 ggatcaaact accaagttcc tgtcgaagtt cgtccagaac gtcgtacaac tttaggactt
    19621 cgttggttgg ttaacgcatc acgtgctcgt ggtgaacaca ctatgaaaga tcgtcttgca
    19681 aaagaaatca tggatgctgc aaacaacaca ggagcatcag ttaagaaacg tgaagatact
    19741 cacaaaatgg ctgaagctaa ccgtgccttt gcacacttcc gttggtaaga ttactctctt
    19801 tttgagactt atcatagcta ctaaacaaat tgtttggtag atagcaatgt tagcaattag
    19861 aacgggtagg tcctgcctat ccgtttttta taaatagtgt tataataaag aagtaaataa
    19921 tttaaaatat aggagaaacc aaatggctcg cgaattttca cttgaaaaaa ctcgtaatat
    19981 cggtatcatg gctcacgtcg atgctggtaa aacaacaaca actgagcgta ttctttacta
    20041 tactggtaaa atccacaaaa tcggtgaaac acacgaaggt gcttcacaaa tggactggat
    20101 ggaacaagaa caagaacgtg gtatcacaat tacatcagct gcaacaacag cacaatggga
    20161 tggtcaccgt gttaacatca tcgatacacc aggacacgtg gacttcacaa ttgaagttca
    20221 acgttcactt cgcgtacttg atggtgcggt aacagttctt gatgctcaat caggtgtaga
    20281 acctcaaact gaaacagttt ggcgtcaagc aacagaatac ggagttccac gtatcgtttt
    20341 tgctaataaa atggataaaa tcggtgctga cttcctctat tcagtacaat cacttcatga
    20401 ccgtcttcaa gcgaatgcac acccaattca attgccaatt ggttcagaag acgatttccg
    20461 tggtattatt gacttaatca aaatgaaagc tgaaatctat actaatgacc ttggtacaga
    20521 tatccttgaa gaagatattc cagcagagta tgttgatcaa gcaaatgaat atcgtgaaaa
    20581 attagtagaa gcagttgctg atactgatga agatttgatg atgaaatacc ttgaaggtga
    20641 agaaatcact aacgaagagt tgatggctgc tattcgtaaa gcaactatca atgttgaatt
    20701 ctaccctgta ttgtgtggtt cagccttcaa gaataaaggt gttcaattga tgcttgatgc
    20761 tgttattgat taccttccaa gcccacttga tattcctgca atcaaaggta ttaaccctga
    20821 tactgacgaa gaagaaacac gtcctgcttc agatgaagag ccatttgcag cgcttgcctt
    20881 caagatcatg acagatcctt tcgtgggtcg cttaacattc ttccgtgtgt actcaggtgt
    20941 tcttaattca ggttcatacg tgttgaatac atctaaaggt aaacgtgaac gtatcggacg
    21001 tatcttacaa atgcacgcaa atagtcgtca agaaattgaa actgtttatg ctggggatat
    21061 cgcagcggct gttggtttga aagatacaac tacaggtgat tcattgactg atgaaaaatc
    21121 aaaagttatt cttgaatcaa ttgaagttcc agaaccagtt atccaattga tggttgagcc
    21181 aaaatctaaa gctgaccaag ataaaatggg tatcgccctt caaaaacttg ctgaagaaga
    21241 tccaacgttc cgcgttgaaa ctaacgttga aactggtgaa acagttatct caggaatggg
    21301 tgaacttcac cttgatgtcc ttgttgaccg tatgaaacgt gaattcaaag ttgaagcaaa
    21361 cgttggtgca cctcaagtat cataccgcga aacattccgc gcttctacac aagcacgtgg
    21421 attcttcaaa cgccaatctg gtggtaaagg tcaatttggt gatgtttgga ttgaatttac
    21481 accaaacgaa gaaggtaaag gattcgaatt cgaaaatgct atcgtcggtg gtgtggttcc
    21541 tcgtgaattt attccagccg ttgaaaaagg acttgtagaa tctatggcaa acggtgttct
    21601 tgctggatat ccaatggttg acgtaaaagc taaactttac gatggttcat accatgatgt
    21661 tgactcatct gagacagcct tcaagattgc tgcatcactt gcacttaaag aagctgctaa
    21721 atcagcgcaa ccagctatcc ttgagccaat gatgcttgtt acaattacag ctccagaaga
    21781 caatcttggt gacgttatgg gtcacgtgac tgcacgtcgc ggtcgtgttg atggtatgga
    21841 agcacgtggt aatacacaag ttgttcgtgc ctttgttcca cttgctgaaa tgtttggtta
    21901 tgcaacagtt cttcgttctg caacacaagg acgtggcaca ttcatgatgg tatttgacca
    21961 ctacgaagat gttcctaagt cagttcaaga agaaatcatc aagaaaaact ctggtgaata
    22021 atttctgaaa acttatgaaa ggtactcgaa agagtatctt tttttctttt tgaaagtggt
    22081 aaattcattg caatttttct cgaaaaaaga tataataatt gtgttgaaag atttttgcac
    22141 atatgtacaa gttaatcttt tcacattggt tagggggatc cctaaaataa aatatttgag
    22201 attttcataa ggaggaaatc actaatggta gttaaagttg gtattaacgg tttcggtcgt
    22261 atcggtcgtc ttgcattccg tcgcatccaa aacgtagaag gtgttgaagt tactcgtatc
    22321 aacgacctta cagatccaaa catgcttgca cacttgttga aatatgacac aactcaaggt
    22381 cgtttcgacg gtactgttga agttaaagaa ggtggattcg aagttaacgg tcaatttgtt
    22441 aaagtttctg ctgaacgcga accagcaaac attgactggg ctactgatgg cgtagaaatc
    22501 gttcttgaag caactggttt ctttgcatca aaagaaaaag ctgaacaaca catccatgaa
    22561 aatggtgcta aaaaagttgt tatcacagct cctggtggaa acgacgttaa aacagttgtt
    22621 ttcaacacta accacgatat ccttgatgga actgaaacag ttatctcagg tgcttcatgt
    22681 actacaaact gtcttgctcc aatggctaaa gctttacaag acaactttgg tgttaaacaa
    22741 ggtttgatga ctactatcca cgcatacact ggtgaccaaa tgatccttga cggaccacac
    22801 cgtggtggtg accttcgtcg tgctcgtgca ggtgctgcaa acatcgttcc taactcaact
    22861 ggtgctgcaa aagctatcgg acttgttatc ccagaattga acggtaaact tgatggtgct
    22921 gcacaacgtg ttcctgttcc aactggatca gtaactgaat tggttgcaac tcttgaaaaa
    22981 gacgtaactg tcgaagaagt aaatgcagct atgaaagcag cagctaacga ttcatacggt
    23041 tatactgaag atccaatcgt atcatctgat atcgttggta tttcatacgg ttcattgttt
    23101 gatgctactc aaactaaagt tcaaactgtt gacggtaacc aattggttaa agttgtttca
    23161 tggtacgata acgaaatgtc atacacttca caacttgttc gtacacttga atactttgca
    23221 aaaattgcaa aataattttt aattaatatg atgtaaaaga gggatttatc cctcttttat
    23281 ttttataaat aaaaaataat acgaatgttt tttcatttta ttatagaaaa ttatcagaaa
    23341 ataaatgaaa aatgaattat attgactata agttaaaata aggtttgatt ttgaaagtaa
    23401 gcagtggtat g
//
LOCUS       DATRSG010000027        23209 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRSG010000027 DATRSG010000000
VERSION     DATRSG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23209
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.027"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..963)
                     /locus_tag="VBD89_001052"
     CDS             complement(<1..963)
                     /locus_tag="VBD89_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEO7135591.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSKNDKILLNSYFKHNLSLKECSQELFI"
     gene            1129..2271
                     /locus_tag="VBD89_001053"
     CDS             1129..2271
                     /locus_tag="VBD89_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO7135592.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            2296..3552
                     /locus_tag="VBD89_001054"
     CDS             2296..3552
                     /locus_tag="VBD89_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO7135593.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            3653..4717
                     /locus_tag="VBD89_001055"
     CDS             3653..4717
                     /locus_tag="VBD89_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7135594.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYNSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(4772..5107)
                     /locus_tag="VBD89_001056"
     CDS             complement(4772..5107)
                     /locus_tag="VBD89_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7135595.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(5296..6324)
                     /gene="queA"
                     /locus_tag="VBD89_001057"
     CDS             complement(5296..6324)
                     /gene="queA"
                     /locus_tag="VBD89_001057"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO7135596.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            6477..7157
                     /locus_tag="VBD89_001058"
     CDS             6477..7157
                     /locus_tag="VBD89_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO7135597.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            7308..8009
                     /locus_tag="VBD89_001059"
     CDS             7308..8009
                     /locus_tag="VBD89_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO7135598.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(8081..9037)
                     /locus_tag="VBD89_001060"
     CDS             complement(8081..9037)
                     /locus_tag="VBD89_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO7135599.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETINFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            9133..9852
                     /locus_tag="VBD89_001061"
     CDS             9133..9852
                     /locus_tag="VBD89_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7135600.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            9925..11076
                     /locus_tag="VBD89_001062"
     CDS             9925..11076
                     /locus_tag="VBD89_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7135601.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            11128..12075
                     /locus_tag="VBD89_001063"
     CDS             11128..12075
                     /locus_tag="VBD89_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO7135602.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            12091..13896
                     /gene="pepF"
                     /locus_tag="VBD89_001064"
     CDS             12091..13896
                     /gene="pepF"
                     /locus_tag="VBD89_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7135603.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVTKGVHND"
     gene            14091..14717
                     /locus_tag="VBD89_001065"
     CDS             14091..14717
                     /locus_tag="VBD89_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO7135604.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            14791..15498
                     /locus_tag="VBD89_001066"
     CDS             14791..15498
                     /locus_tag="VBD89_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO7135605.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            15559..16488
                     /gene="prsA"
                     /locus_tag="VBD89_001067"
     CDS             15559..16488
                     /gene="prsA"
                     /locus_tag="VBD89_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO7135606.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            16730..17215
                     /locus_tag="VBD89_001068"
     CDS             16730..17215
                     /locus_tag="VBD89_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO7135607.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            17231..19849
                     /gene="alaS"
                     /locus_tag="VBD89_001069"
     CDS             17231..19849
                     /gene="alaS"
                     /locus_tag="VBD89_001069"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO7135608.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            19931..20647
                     /locus_tag="VBD89_001070"
     CDS             19931..20647
                     /locus_tag="VBD89_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO7135609.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            20735..21553
                     /locus_tag="VBD89_001071"
     CDS             20735..21553
                     /locus_tag="VBD89_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO7135610.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
     gene            21754..21942
                     /locus_tag="VBD89_001072"
     CDS             21754..21942
                     /locus_tag="VBD89_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135611.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
     gene            21974..22267
                     /locus_tag="VBD89_001073"
     CDS             21974..22267
                     /locus_tag="VBD89_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135612.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            22312..22527
                     /locus_tag="VBD89_001074"
     CDS             22312..22527
                     /locus_tag="VBD89_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135613.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            22530..>23209
                     /locus_tag="VBD89_001075"
     CDS             22530..>23209
                     /locus_tag="VBD89_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO7135614.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFNIFGQDKY
                     LSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLRH
                     SYGMTFVSISIVLSIFNTLFNYKLNIFDDSVTLVIPIYDLSLLFVLLGLHIYFLKVYR
                     NIRGIKMTVAPTLKELKNNVLQLDEAELESNYKMCFDIVMNLSGFIFPTIYFVLFFIS
                     FVFQKVEIV"
BASE COUNT         7631 a         3631 c         4496 g         7451 t
ORIGIN      
        1 gataaaaagt tcctgactac attcttttaa tgatagatta tgcttaaaat atgagttaag
       61 caagatctta tcatttttag ataagtttac aagcgcttta tttagcacat attgtttaat
      121 attagaatca atacttgtta ataacagttc aagatcaagg tcgtcgacga ggttgacctt
      181 ttgttgacct tttaaagctt tcagggctaa gatagacgtt tcatatgatc tttttaaaag
      241 tgataaagat gatttttgtc cgataccaac tttatagaga cccttttcgc attcaaactt
      301 tgataggata tttggataat aatagtcaaa catctggtca gaaagtaaaa gccagtattg
      361 attgggataa ttaaaggtat aaatagcagt gtcaagttta tcaaaaaagt tcaagagtga
      421 catttcagat tgacttaaga gtaatttttg tttttgacta ttgatttcaa aagtgagtag
      481 acgatagtca gttttttctg ataggtggta ttggtttaaa aattgattga ggtaatagta
      541 gtctaactct ctgccttcaa caagatggtg taagacaata gaagcttcgt tttttcgccc
      601 gaaatccaga taatctaatt catgctcttt taagatgagt cgagtcattt tttgagcaag
      661 taaagcatat tttcctactt ggttggggtt tccactaatc ccaatagtag ctaataattc
      721 acaattataa tagaaaggaa tattgatccc tgcttgtgtc ccaaaataag attcttgatc
      781 tgttacttca atcatttgtc ctgtctgtgc tacttttaaa ccaatttcat ggaattctcc
      841 aacacgtttt ggattggtac tagcgaaaat gattcctttg gtattaataa aattaatatc
      901 ttgttgacaa acatctttga tagaggtaac gatttgttgt gcgagttgtt ttcttaattt
      961 cattcagctt accttttgtt atatataaca aatatcatat cataaaaacg ctaacatttg
     1021 ttctatagtc tatatttttt gttatttgat tttgttacta taattgagtg atataaaata
     1081 gtgaaaaaat aacaaagatt cacaaaatat tttcaaggag gttttcaaat gaaggtagta
     1141 gtagctattg attctttgaa agggagtctt tcttctttag aagctggcaa tgctataaag
     1201 gagtcaatta atgaagtaat cccaggggcg gatgtggaag tacatccatt agcagacggg
     1261 ggagaaggaa ctgttgaagc tttgacgcta ggtatggggg ggacgatcga aataatccct
     1321 gttaaggggc ctcttggaga aaaagtacat gcgagttatg gtattattcc acagcgtcag
     1381 ttagctatta ttgaaatggc tgcagctgct ggtattaccc taattgctac tgaagagaga
     1441 aatcctctcc acacaaccac ttatggagtt ggagaaatga taaatgatgc tataagtaaa
     1501 ggctgtaggc actttattat tggtattgga ggtagtgcta ctaatgatgg tggggcaggt
     1561 atgctacaag ctttgggata tgcattatta gataaagata atcaagaaat cagtttggga
     1621 gcacaaggat tggcagattt aaaatctatc tctactgata aggttattga ggaattaaaa
     1681 gaatgcgatt ttaaaattgc atgtgatgta acaaatcctt tatgtggtgc tcaaggttgt
     1741 agttcgattt ttggaccgca aaaaggtgct gatgaagata tgattacaaa aatggatact
     1801 tggttaagta attatgctac tttagctact tcagttagtg aaaaagcaga tgcaactatt
     1861 gaaggaacag gtgcagcggg tgggcttgga tttgcctttt tagcctttac aaatgccacc
     1921 ttagaacctg gtattgatat tattctatca gaaattaaca tagaaaaagc gatttctgaa
     1981 gctgatttag tagttactgg tgaaggtcgt ttggatgggc aaacggtgat gggaaaagct
     2041 ccaattggcg tagcaaaatt agctaaaaaa tatggtaaga aagtagtggc attctctgga
     2101 tctgtcaccg aagatgctat actttgtaat caacatggta ttgatgcttt ttttccaatc
     2161 gtacgacgcc ttatcagctt agatgaggct atgtctaaag aggtagctta taaaaatatg
     2221 aaagaaactg ctactcaagt ctttagatta atcaatttgt ataatcatta atattgaaag
     2281 gaattctgat aaattatgga agctacattt acaacaacag gggctttaat aggattagcg
     2341 cttgctattt tactgattat caaaaaagtt catcctgcct atagtctcat tcttggagca
     2401 ttagttggtg gtttgattgg tggcggtgat ttagttacaa ttgttaatac tatggtatta
     2461 ggtgcgcaag ggatgatgtc atccatatta cgcattttaa cgtcagggat tttagcgggt
     2521 gctttaatta aaaccggatc agctgaaaaa attgcagaaa gtatcattaa aaaattagga
     2581 caacagcgag ccatcacagc tttagccatc gcaacaatga ttatttgtgc agtaggtgtc
     2641 tttattgata ttgcagttat tacagttgct ccgattgcat tagcaatagg taaaaaagct
     2701 aatctttcta aatctagcat actgttagca atgattggtg gtggtaaggc tgggaatatt
     2761 atttcaccca atcctaatac aattgctgct tcagaagcat ttaaggttga tttaacaagc
     2821 ttaatggtac aaaatattat tcctgcaatt gcagctttag tagttacgat tatattagca
     2881 aagattgttt ccaagaaaaa taacgatatt tcttatgata gtgaggagca agtgggctct
     2941 gatctgccag cattcttacc ggctatttct ggtccacttg tggttatctg cttattagct
     3001 cttcgtcctt tatttggaat cactattgat cccttgattg cactgccgtt gggtggtcta
     3061 attagtattt tagcaacagg ttacctaaaa gaaacggtac cgtttgttga atacggttta
     3121 tcaaaagttg ttggcgtttc aattcttttg attggaacag gtacactttc aggtattatt
     3181 aaagcatcta atctacaatt tgatatgatt catctccttg aatttttaaa tatgccgacc
     3241 tttatccttg ccccactctc aggtattttt atgggagcag caacagcatc aacgacttct
     3301 ggaacaacta ttgcttcaca aacgtttgct gaaaccttga ttaaatcagg ggtaccggcg
     3361 gtttcgggtg cagctatgat tcacgcaggt gcgactgttc tggattcgct acctcatgga
     3421 tccttcttcc atgcgacagg cggagctgtc aatatggcta taaaagaacg tatgaaacta
     3481 attagctatg aagctttgat tggattaact agcacaatag ttgctgtcgt ttactattgt
     3541 ttctttgggt aaaatttgga tgaatacgtg atgtttatta tcactgtttg aatagaattc
     3601 tcattgattt tttctaattt tcttactata ataaattagt aggagaggtg ttgtgaaaca
     3661 aacaaagaaa gaatttattt taggattaac cattattatt acccttactt tattaatcat
     3721 gctgattttt ttagtaatgt ttaataagtc cagtaactct aagttgaagc agacaaaaga
     3781 tagaagaata ttatctactt caaccacaag gacatccagt agctcacatg atcaaaaaat
     3841 tgtagaaaag tctcttgaaa aaaagaaaat ggtcatggaa caagatatgg aattgatgaa
     3901 tgcctatagg ctctattatg attatgccca tttgtccttg aaagaggttg ttctggccta
     3961 tatgtctgaa tatggcattg aaaaaaatag tgttgctttt tcttataaaa atttaaaaac
     4021 aggacaaagg gacgctatga atgatactca ggctatgaca gcagggagca cctataagtt
     4081 acctcttaac atgttggtag tagatgcagt ggtcaagggc aagttgtcca tggaagaacg
     4141 ctttgatatt accgagactg actatgaata tattggtgaa cataataatt atgttgctgc
     4201 tttctcgggg gcaatgtcta ttgatgatat gcaaaaatac tctttagttt attcagaaaa
     4261 tacacccgcc tatgctttag ctgagcgtat tggtggcatg gatagtgctt attcaaagtt
     4321 tggtcgttat ggccaatcta aaggagacat caaaaatata caaaaaaacg gtaataaagt
     4381 gacaacagat tactatattc aagtgctaga ctatctctgg aaacatcgta aaaagtataa
     4441 tagtttaatt acttatttag aagaagcttt tccaacggat tattatcgtg ctttgatacc
     4501 atctgatgtt gttgttgctc aaaagccagg gtatgtccgt gaggcgttga atgttggagc
     4561 aattgtcaaa gaagaagtgc cttatatcgt tgctatttat actgcaggtt taggtggctc
     4621 tactcaggaa gattcagaaa ttaatggagt gggactatat cagctagaac aattatgttt
     4681 tgttattaat caatggcatc gtgttaatat gaattagaaa taaatctatc aaaataataa
     4741 aaacctctta gattaactat ctaagaggtt tttataaatg tgattttata acttgattaa
     4801 gtgaagaaag ggcagaaaca gttttttgat aagattcagg actaatttct gagaaaaaat
     4861 acccgcacga ttgcaccgca ttgacaacgg catcttgtga ctcaatacct ttgttactta
     4921 gatgaataat gagttgcctt ttgtcatctc ttggtttttc tcgtacaatc cacccacgat
     4981 tttccatacg ctttaaaact ggagttaggg tattcgaatt aagagatagt gcataactaa
     5041 gttccttcat atgccgatga tcctcttccc aaaggactaa caagataacg tattggagat
     5101 aagtcaattg aaattcatcc aaagcttttt ggtaaaattt attgaaaagt cgatgtgttt
     5161 cactaaccgt aaaacataat cgctcgtcta aaatacttcg attactcatt atcatattgt
     5221 agcattttgg aggcgttata gtatgtcaat cgtgttaaaa aacgataaaa ctttttccta
     5281 attgaaagcc tattcttact taacaaacat agcatcacca aaactgaaga aacgatatcg
     5341 ttcttcaact gcatggttgt aagcttctag tacaaagtca tgtccagcaa atgctgatac
     5401 taacatgaca agagtagact ttggtaagtg aaagttggtt gaaaaagcgt ctacaacctt
     5461 gaactgataa ccaggcttga taaaaatatt tgtccaccct gagtcggctt ttaattcacc
     5521 attgaattta gaaccaattg tctctaatgt gcgaatagaa gttgtaccaa cagctacaat
     5581 acgtccacca gattctttaa ctgcattgag agtatcagca gcttctttag ataattggta
     5641 aaactcagaa tgcatttcgt gttcatcaag attatcaact gaaacaggac ggaaagttcc
     5701 aagtccaacg tgaagcgtca aatagacaag cttcacacct tttgtttcaa ttttttccaa
     5761 tagttctttt gtaaaatgaa gaccggctgt tggtgcagca gcagaaccat tttcttttgc
     5821 atacacagtc tgatagcggt cacgatcttc tagtttttca tgaatatatg gcggaagtgg
     5881 catttcgccc aaactttcaa ggacttctag aaaaatacca tcatatgaga attctacaat
     5941 acgcccacca tgttctaatt cctttgtgac tgttgctatt aaccgaccat caccaaatga
     6001 aactctgttc cctacgcgta agcgtttagc gggtttagca agcacttccc attgatcgcc
     6061 ctctgtattt ttgagaagta ataactctac atggccgtgt gtatctggct tttcaccata
     6121 aaggcgagcg ggcagaacac gtgtgttatt cattactaaa gcgtcaccag gcttgagttc
     6181 atctaagata tgatcgaaat ggctatctgt catcgtctta ttcttatgat caatgactaa
     6241 tagtttagaa gcgtctcttt tttcaagtgg tgtttgggca ataagttctt ctggtaaata
     6301 aaaatcaaaa tcgtttgtat tcatgatttc tcctatctat aagtaagctt ttctattata
     6361 tcacgttcgg taaaaatgac ccaactaatc ttcttattta gaatgattat aaaaaataat
     6421 tgtaatcatt ctaaataagt ggtataataa ttgtcagaaa gtgagaagta gagaagatga
     6481 aagaagcaaa aaatttttcc caacaattga atattttaag agctggtgtt cttggtgcta
     6541 atgatggtat catctctgta gctggtgttg ttattggtgt tgcgagtgca actcataacc
     6601 tttggattat ttttttatca gcagcttccg cgattttagc tggtgcattt tctatggctg
     6661 gcggggagta tgttagtgtt tctactcaaa aagatactga acaagcagca gttgcgcgtg
     6721 aagagaaact actagaaaat aatccagaac ttgccaaaaa atccttagtt gatatctact
     6781 tagcaaaagg agaaagtcac gaacatgctc aatggctagt tgacaaggct ttttcaaaga
     6841 atgctattga gcatttagtt gaagaaaaat atggtattga gttcggtgaa tatacaagcc
     6901 cctggcatgc tgctatatct agttttatag cttttgccat cggatcaatt tttccaacta
     6961 taactattct gttattacca ttttcggtac gaattgtagg aacggtaata attgttattg
     7021 tttcactatt atcaactggt tatgtaagtg ctaagttagg ccaagcccct acagtacctg
     7081 ccatgcgtcg aaatgtgatg attggttgtt tgacaatgtt ggcaacctat gtcattggac
     7141 aattattttc aatataaaat attaatcgtg gagttattgt ctaaactaga atggacagta
     7201 attcttttta gttttataac gaaattaaga agtttttatt gacaaaaatt ggtatatacc
     7261 atataatata atcataaagg tctataccaa ataaaaggag agtaaacatg agagttatta
     7321 cagttaagaa cgacattgag gggggaaaaa ttgcatttac tcttttagaa gagaaaatga
     7381 aaactggtgc tcaaacttta ggattggcaa caggaagtag cccaatcacc ttttacgaag
     7441 agattgtaaa aagtaacctt gatttttcaa atatggtttc tattaacctt gatgaatatg
     7501 ttggaattgc cgcaagtaat gatcaaagtt actcttattt tatgcataaa catctttttg
     7561 atgctaaacc ttttaaggaa aataaccttc caaacggctt ggctaaagat ttgaaaaaag
     7621 aaataaaacg atacgatgct gtcataaatg ctaatccaat tgattttcaa attcttggaa
     7681 ttggtcgtaa cggtcatatt ggttttaatg aaccaggtac accttttgat attacaacac
     7741 atgtggttga cttagctcca agtacaattg aagctaatag tcgtttcttt aatagtattg
     7801 atgatgtacc aaagcaagcc ttatcaatgg gtataggttc tattatgaaa tcaaaaacaa
     7861 tcgtattagt ggcgtatggt attgaaaaag cagaagcgat tgcaagtatg attaaaggac
     7921 ctatcacaga agatatgcca gcaagtatcc tccaaaaaca tgatgatgtt gtgattattg
     7981 tggatgaagc agcggcatca aagttgtaaa gtaaataata aaaaagtaag gatgaatctc
     8041 aatgagaaaa aattcattct tactttttta ttatgcttta ttaaattgtc cagtctaagt
     8101 taggtccagc tggaataata ccggtagggt taatggtttt atggctaccg taatagtgtt
     8161 ttttgatatg atcaaaatta attgtctcag caattccagg taggttgtaa atacgttttg
     8221 tataatgcca taaatgtggg taatcatgta gcgcttttaa attacattta aaatgaccat
     8281 aataaacggt gtcaaagcga actaaagttg tgaaaaggcg aatatcagct tcggtaaatt
     8341 gttctccaac aagataatgg ttatcagata agtgtttttc taactgatct aaagctgtga
     8401 ataaggtttc aacttccttt tgataaacgt tctgagaagt agcaaaacca gctttataga
     8461 caccattatt tatttttgga taaataaaat tattcatttc gtcaatttgt ccttgtaagg
     8521 aatcgggata gtaatcgtca gtatttccag taatatgatt aaatgcagta tttaacatgc
     8581 gcataatttc agatgattca ttgttaacga ttgtatggca ttttttatcc caaagaacag
     8641 ggactgtcac gcgtcctgta taatcacttt gacttgcttg atagatttgg taaaggtatt
     8701 ggctttggtt aatcatatca ggaataacgc ctttatattc ttgaaaagtc caaccattct
     8761 ccaacattaa agggttaaca atggagatag agatatgaga ttctagattt ttaagctttc
     8821 tcatgattaa tacacgtgat gcccaagggc aggctagtga aacgtagaga tgatatcgtc
     8881 cagattctgc tttaaaacct gcatccccac taggccctgc tgaaccatct ttagtaaccc
     8941 aatgacggaa ttgtgtaacg gttcggacga atttaccacc agttgatgca gtatcatacc
     9001 attgatcaac ccatttgcca tctactaaaa gtcccatgat aatctccttt gtttttctat
     9061 tactaattag tagtataaag attttaaaag gaattagcaa atgtttaact tacagtatgg
     9121 taaactaagg gtatgcgttt agataaatta ttaggtcagg caggatttgg gtctagaaat
     9181 caggttaaga aattaattcg ttctaggcaa gtttctgtag atggtcaaat tgttacaaag
     9241 gataatgtta ttgtagatag tggtctacaa tcgatttttg taggaaaaga gagagtctgc
     9301 ctaaaagaaa gttcttatta ccttctttat aagccatcag gagttgtttc agcagttaga
     9361 gattcagaac ataaaacagt catagactta attagcgaaa aagataaggt ggagggattg
     9421 tatccgattg gtcgtctaga tcgtgatacg gaagggcttt taataatcac gaataatggt
     9481 ccactgggtt accgaatgtt acatccaaaa caccacgttg caaaaacgta ttatgttgaa
     9541 gtaaatggtt tcttggaaag agatgctatt accttttttg aagaaggagt tgtatttgac
     9601 gatggaacaa agtgtaaacc agctgaatta acgattgata cggctaataa tgacaaaagt
     9661 accgcaagaa taactatcac agaagggaaa tttcaccaag taaaaaagat gtttcttgca
     9721 tatggcgtta aagttatata cttgaggcgt atttcttttg gtgacttgag gcttgatatg
     9781 aatttgaaac caggtcagta tcgtagattg agagattcag aggcagctat cttaaaaagg
     9841 tatttagatt agtgaaacga ctcacttgac cttgcttagt attcttcata tacttataag
     9901 tgattatttt aaaggagaaa actattgaat tcaaaacttt cattgaaatt gactatttta
     9961 tcgatttcta tctttttgat gtctcactta gcaattgctc ctgcaattcc taaattgtat
    10021 gccttttacc atactcaaaa ccctcatatt ggactagctt cagtagagag tttagttact
    10081 attccagcta tgatgattac tatttttgtt attttgagta attttgtagt cacaaaactt
    10141 ggcaaaaaaa acacggtctt acttggttta tgtttaatct taatgtctgg gtttatctca
    10201 ttttttacaa gtaatttttc acttgcgatg gctagtcgac tacttttggg tattggtatt
    10261 ggtctctata attcgctatc aattagtatc ataacggatt tatatgaggc tgatgagaga
    10321 gccagtatga ttggtcttag aacagcatca cttaatatcg gtaaagcctt aactacattt
    10381 attgttggat tggttttagc tattggtgtg aattatattt atttggttta ccttctggtt
    10441 attcctgttt tctttttctt ttggaaaaat gttccagagg ttgaaaatca aacacatacc
    10501 cttaaagcta gtacaacatt tgatactaaa gcggctttgt tgatgctcat cacgttctta
    10561 gtaggtattg cttatatagg agcaactgtc aaaattccaa ccttactagt gacaaaatat
    10621 cactacgcga cgagcttctc tagtaatatg ttaactctcc ttgcttttag tggtatttta
    10681 gttggatctg tatttggaaa attagtcaaa gcattccaag agaagacgct attaatcatg
    10741 attctagcta tgggaatagg taatgtgcta tttgctttag ctaataatca gatcattttt
    10801 atcgtagcta gtattttaat aggagctagt tttgttggca ccatgtcttc tgtattcttt
    10861 tatatttcaa aaaactatgc aaaagaacat aataacttta tcacaagcct tgccttaaca
    10921 gctggtaata taggagttat tttgacaccg cttattttaa cgaaacttcc aagccagcta
    10981 catctggaac cttttataac accattttta ataacaagtg ggctgatggt gattaatgtc
    11041 tttgtttacc tagttttaat gtcaaaaaat aaataaaaag ttatccgaaa ggatagcttt
    11101 tttcgaatag taaaagtagg agaaaaaatg ttaattgcta aagacaaaca aggaaattta
    11161 attaatctac tcgagtctca tccgggaaag ggacagtatt tttgtccaac ttgttgttca
    11221 gctgtaaggt taaaagcagg tcgaatcatg agaagacact ttgcacatat tagtctaaaa
    11281 aattgtcagt ttcatcatga aaatgagtct aatgagcact tgcaattaaa agcaaagtta
    11341 tatatgagtt taagtagaga gaatgaaact atgctcgagc atcatttgcc agaaattaat
    11401 cagattgcag atttatttgt gaatgagaca ttggctttag aagttcagtg tagccgacta
    11461 tcagagcagc gactcaggga gcgaacaaaa gcatatctac aggcagactt tcaagtgaga
    11521 tggttactag gggaaaaact ctggttaaag catcgtttaa caaatctaca taaacaattt
    11581 ttacaatttt ctcaaagtat cggatttcat atttgggagt tagatttgag gttagaagta
    11641 ctgagactaa aatatttgat ttacgaagat ttacgcggac atgtctatta cttaagtaaa
    11701 acttgccctc ttagtggaga cgttctagca tttttgaagt ggccatacca atcaaaaaat
    11761 ctaaattttt ataaggttaa acaagacaga aatatcagag attatgtgcg tcagcagtta
    11821 cgctatggca atcaattttg gttacgaaaa caagaaaagg cctacctttc tggtcagaat
    11881 ctattaaccc aagagctaat gatgtttttt cctcaaatac agccaccacg tgtagatact
    11941 gatttttgtc aaataacaaa ttctctgaca tcgttttatc agaattttac aaattattat
    12001 caaaaaaata agaataactt ggaccaaaca ctttatcccc ctgtatttta tgataaaata
    12061 gtcactaaga attaaactgg aggatacaaa atgtcagaca atcgctcaca tattgaagaa
    12121 aaataccaat gggatttaac tactgtcttt gcgactgatg agttatggga aactgaagtg
    12181 gtagagctta ctcaagccat cgatgacgca aagggttttt caggtcatct gttggactca
    12241 agccaatctt tattggagat tacggaggtt gaactagagt tatctcgccg tttagaaaaa
    12301 gtttatgtct atgcttcaat gaaaaatgac caagacacca ctgttgcgaa atatcaagaa
    12361 tttcaagcga aagcgacagc gctctatgca aaatttagtg aaacattttc gttttacgag
    12421 ccagaactat tgcaactttc agagtctgat taccagtcat tccttttaga aatgcctgac
    12481 ttgcaaaaat atgatcattt ctttgagaaa atatttgcaa ataaacctca tgtcttatct
    12541 caaaacgaag aagaattgtt agctggtgct agtgagattt ttggagcagc tggtgagaca
    12601 tttgaaatcc ttgataatgc agatatggtc ttcccagttg taaaaaatgc aaaaggtgag
    12661 gaagttgagc ttacacatgg taatttcatt tcccttatgg aatctagcga taggacggtt
    12721 cgaaaagaag catatcaagc tatgtatagc acatatgaac aattccagca tacttacgct
    12781 aaaacattac aaacaaatgt gaaatctcaa aattttaagg ctcgtgtgca tcattatcaa
    12841 tcagcacgcc aatcagctct atctgccaat tttattccag aagaggtcta cgaaactcta
    12901 attaagacag ttaatcatca tttaccgctc ttacatcggt atatgaagtt gcgtcaaaaa
    12961 gttcttgggc tagatgattt gaagatgtat gacgtatata cacccctttc tcaaatggat
    13021 atgagtttta cttatgatga agctctaaaa aaatcagaga aggttctggc tatttttggc
    13081 gaagcatatt ctgaacgtgt tcatcgtgct tttactgaac gttggattga tgtccatgtt
    13141 aacaaaggaa agaggtcagg cgcttattca ggtggatctt atgacactaa tgctttcatg
    13201 cttcttaact ggcaagatac tttagacaat ctttatacgc tggtgcatga aactggacat
    13261 agtctacact cgacgtttac tcgtgaaaat caaccttatg tttatggtga ttattctatt
    13321 ttcctagctg aaattgcatc aacaacaaat gaaaatatat taacagaaac ccttttgaaa
    13381 gaagttaaag atgataaaaa tcgctttgca attctcaatc attatttgga cggtttcaaa
    13441 ggaacaattt ttagacaaac acagttcgct gaattcgaac atgccattca tgtagctgat
    13501 caggaggggc aagtactaac tagcgaatac ttaaacaacc tttacgctga gcttaatgaa
    13561 aaatattatg gtttgactaa agaagataat cattttattc aatatgaatg ggcaagaatt
    13621 ccacactttt actacaatta ttatgttttc caatatgcaa ccggcttcgc agcagcaaat
    13681 tatttagcag agcgtattgt aaacggtaat ccggaggata aagaggccta cctaaattac
    13741 cttaaagctg ggaattcaga ttaccctcta aacgtcattg ctaaagccgg tgttgatatg
    13801 actagtgcag actatctgga tgcagctttt agagtatttg aagagcgttt agtggaactg
    13861 gaaaatcttg taacaaaagg cgtccataat gattaagttg aaaatatagc tattgaagta
    13921 aaaccagtca actagactgg tttttgatag tgatcagtta ttttatagtt tgttttgaga
    13981 gtggttttgg taaagttagt tagtggaaaa ctcaataggt ggaaatacct acccaagcta
    14041 tgatttttat gagaccaaaa ctgctattgt caaaaaagaa gggaaatagg atgaaaaagc
    14101 ttacttttat ttgggattta gatgggacat taatagattc gtatgtacca attatggaag
    14161 ctcttgaaga aacctatcgt cattttggct taatatttga taaagaatta atccatgaat
    14221 atattttaca ggaatcagtg gggcaattat tggtaaacct ttcagaggaa gagcaaatac
    14281 ctcatgaaaa actgaaagca tattttacaa aagaacaaga aagtcgagat tctaaaatac
    14341 atttaatgcc atatgcaaaa gagattttag aatggaccaa agaacaagat attcccaatt
    14401 ttatgtatac acataaagga gcaagtacgc attcagtgtt ggaaaccttg cagatctctc
    14461 attattttga tgaaatttta actggtgttt cgggattcga gcgaaaacca catccacaag
    14521 ggattaatta tttagttaaa cgatattctt tagataaatc aatgacttat tacataggag
    14581 atcgtccact agatttggag gttgctcaaa atgctggtat aaaatccata aacttaaggt
    14641 tagagaattc caaagaaaac tataatattt caagtctcaa agatataata tcacttgatt
    14701 tcactcgttt ggattaaatc attctcagag ttagacattt tttgctataa taaaaaagtt
    14761 attatttaaa atagaaaaga ggagccaaaa gtggttcaat cttacagtaa aaatgctaat
    14821 cataatatgc gccgtccagt agttaaagaa gaaattgttc aatatatgcg tcagcaccaa
    14881 aaacaaaata atggctgttt agcagagtta gaagcatttg ctaaacagga aaatatcccc
    14941 attatacctc atgaaacagc gacttatttt cgttttttga tgcaaacatt acaacctaag
    15001 cacattttag aaatcggaac agctattggt ttttcggccc ttttgatggc agaaaatgct
    15061 cctgaagcaa agataacaac aatcgatcgt aatgaagaga tgattgcctt ggctaaggaa
    15121 aactttgcaa aatatgataa tcacaatcag ataacccttt tggaaggcga tgctgtggat
    15181 gtgttacaaa cattggataa atcttacgat tttgtattta tggattctgc taagtctaaa
    15241 tatatcgttt ttttacctca agtattaaag catttagatg ttggaggagt agttgtttta
    15301 gatgatattt tccaaggtgg agatattgca aaacctatcg atgaagtgag aaggggtcag
    15361 cgaactatct accgaggtct gcaacgttta tttgactcga ctttgcaaca tcctgattta
    15421 actgctactt tggtaccttt gggagatggt ttattgatga ttcgtaaaaa tgcagatcat
    15481 attgtactag aagattaagg catttttaag aaattatgat ataataatgg agttaatgac
    15541 aaaaaggagt tataaatcat gaaaacaaga tcaaaacttg cagcaggctt tttgacactt
    15601 atgtcagttg ccacattagc tgcctgctca ggtaaaacat caaatggaac aaatgttgtt
    15661 acaatgaagg gcgacactat tacagtctct gatttttatg atcaagtaaa aacatcaaaa
    15721 gctgcacaac aatcaatgct tacattgatc ctctcacgtg tttttgatac acagtatggt
    15781 gataaagttt cagataaaaa agtatcagaa gcttataata agacagctaa aggctatggt
    15841 aattcatttt caagcgcact ttcacaagca ggtttgactc cggaaggtta caaacaacaa
    15901 attcgcacaa ctatgcttgt ggaatatgct gtaaaagaag cagctaagaa agaattaaca
    15961 gaagcaaact ataaagaagc atataaaaac tatactcctg aaacttctgt acaagtaatc
    16021 aaattggatg cagaagataa agctaaatct gtccttaaag atgtaaaggc tgatggagct
    16081 gattttgcaa agattgcaaa agaaaaaaca acagctactg ataaaaaagt tgagtataaa
    16141 tttgattctg cagggacaag cctccctaaa gaagttatgt cagcagcctt taagctagat
    16201 aaaaatggtg tttcagatgt ggtttcaacg gttgattcaa caacttataa aacaagttac
    16261 tacatcatta aagtaactga taagacagag aaaaaatctg attggaaatc ttacaaaaat
    16321 cgcttaaaag aagttattct taaggataaa acaagcgata gatccttcca aaataaagtg
    16381 atttcaaaag ccttagaaaa agctaacgtt aagattaaag ataaagcatt tgcaggcatc
    16441 ttatcacaat atgctacaac aagtggttca tcatcactta aaaaatagtt gaccaagtct
    16501 taaaaagact gtataattaa tactattgag aatgaaattt caacgattag atacagaaaa
    16561 gtgacggttg ctgcgagtca tggataatca gaaaaagatg ctactcaatg gggtaaaact
    16621 aaaaataaat gagaatgaca agttcattga atgaaggtgg taccgcggtt ttcgcccttc
    16681 gtaatggact tgtcttttgc tatttgtcaa ctttcgagga ggaagagata tggctacttt
    16741 tcaaattaaa gaaaaaatgt tttctctagg cggtaaattt actattacag acctgactgg
    16801 gcttccctgt tatcacgttg aaggaagttt gttccctcta cctaagactt ttaaagtctt
    16861 tgacgaagag gaacatctca ttagtcagat tgagaagaag gttttaagtt ttttaccaaa
    16921 gtttaatgtg acattagcca atgggaatca tttcacgatt aagaaagatt tctcattttt
    16981 gaaacctcat tacaccattg aagaccttga tatggaagtt aagggaaatt tttgggatat
    17041 ggatttccaa ctcttaaaag acaatcaagt tatagcaaac atatctcaac agtggtttcg
    17101 tatgacttca acttatcagg tagaagttta cagtgaaact tacaacgatt tgacaatttc
    17161 gcttgtcata gcgatcgact atgtcaaaga gttagaaaaa aatgcatcaa actaatttta
    17221 ggagagaata atgaaagaat tatcgtctgc acaaatccgc caaatgtggt tggatttctg
    17281 gaaatccaaa ggacattccg ttgagccttc agctaacttg gttcctgtta atgacccaac
    17341 gcttctttgg atcaactcag gtgttgcaac cttgaaaaaa tattttgatg gttcagtgat
    17401 tccagaaaat ccacgtatta ccaatgcaca aaaatcaatt cgtactaatg atattgaaaa
    17461 tgttggtaaa acagcacgtc accatactat gtttgaaatg cttggtaact tctcaattgg
    17521 agactatttc cgtgatgaag ctattgagtg gggatttgaa ctcttgacaa gtcctgaatg
    17581 gtttgacttc cctaaagaca agctctacat gacttattac ccagatgaca aggattcata
    17641 taaccgttgg attgcttgtg gcgttgaacc aagtcacttg gtgccaatcg aggataactt
    17701 ctgggaaatc ggtgctggtc cttcaggtcc agatactgag attttcttcg accgtggtga
    17761 agattttgat ccagaaaata ttggacttcg tcttttggct gaagatatcg aaaacgatcg
    17821 ttacatcgaa atctggaaca ttgttctctc acaattcaat gctgacccag ccgtaccacg
    17881 ttcagaatac aaagaattgc caaacaaaaa cattgatacg ggtgcgggtc ttgaacgtct
    17941 tgcagctgtt atgcaagggg caaaaacaaa ctttgaaact gaccttttca tgcctatcat
    18001 ccgtgaagta gagaaattgt caggtaaaac ttacgatcca gatggcgaca acatgagctt
    18061 caaggttatc gctgatcaca tccgtgcgct ttcatttgcc atcggtgatg gtgcgcttcc
    18121 tggaaatgaa ggtcgtggtt atgttcttcg tcgtcttctc cgtcgtgcgg ttatgcacgg
    18181 tcgccgtctt ggcatcaacg aaactttcct ttacaaattg gttccgactg ttggacaaat
    18241 catggaaagc tactatccag aagtgcttga aaaacgtgat tttattgaga aaatcgttaa
    18301 acgtgaggaa gaaacatttg ctcgtactat cgatgctggt agcggtcact tagattcatt
    18361 gcttgcgcag cttaaggctg aaggtaaaga cacacttgaa ggtaaagata tcttcaaact
    18421 ttatgatact tatggattcc cagttgaatt gacagaggaa ttggcagaag atgcaggcta
    18481 taagattgac cacgaaggct ttaagtcagc catgaaagaa caacaagacc gtgcgcgtgc
    18541 agctgttgtt aagggtggtt cgatggggat gcaaaatgaa accctagctg gtattgttga
    18601 agaatcacga ttcgaatacg acacatatag tcttgaatca agtctttcag ttatcattgc
    18661 tgataatgaa cgtactgagg ctgtttcaga aggtcaagcc cttcttgtct ttgctcaaac
    18721 accattctat gctgaaatgg gtggacaggt tgctgacaca ggtagaatca aaaatgataa
    18781 gggtgacaca gttgctgagg ttgttgatgt tcaaaaagca ccaaatggtc aacctctaca
    18841 cactgtaaac gttttggcat cactttcagt tggaacaaac tacacacttg aaatcaacaa
    18901 agagcgtcgt ttggctgttg agaaaaacca cacagcaact cacttgctcc atgcagccct
    18961 tcacaatgtt atcggtgaac acgcaactca ggctggttca ttgaacgaag aagaattctt
    19021 gcgctttgat tttactcact ttgaagcagt aagcaatgag gaacttcgtc acattgaaca
    19081 agaagttaat gagcaaattt ggaacgctct tacaatcaca acgactgaaa ctgacgttga
    19141 aaccgcaaaa gagatgggag caatggcact ttttggtgag aaatatggta aagtggttcg
    19201 tgtggttcaa attggtaatt attctgttga actttgcggt ggaactcact taaataattc
    19261 ttcagaaatc ggtctcttca agattgtcaa agaagaaggt attggttcag gcactcgtcg
    19321 tattattgca gttactggta gacaagcttt tgaagcttat cgtaaccaag aggatgcact
    19381 aaaagagatc gctgctactg taaaagctcc gcaattgaaa gatgcaacag ctaaagtaca
    19441 agctcttagc gattcacttc gtgatcttca aaaagaaaat gtagagctta aagaaaaagc
    19501 agcagcagca gcagctggtg atgtctttaa agatattcaa gaagctaagg gcgtgcgctt
    19561 cattgctagt caagttgatg ttgcagacgc aggtgcactt cgtacatttg ctgataactg
    19621 gaaacaaaaa gactactctg atgtgcttgt tctcgtagca gctattggtg agaaggttaa
    19681 tgtccttgtt gcaagcaaaa ctaaagatgt ccacgctggt aacatgatca aaggattggc
    19741 accaattgta gcaggtcgtg gtggtggtaa accagacatg gctatggcag gtggtagcga
    19801 tgcaagtaaa attgcagagc tgctagcagc agttgcagaa aatttataag aagtacctct
    19861 catttagtta gtaaataaaa caggcaaagg gcagaacatc gttctgttcc tttgtgtata
    19921 taggagaaat atggttggtt taggaacagt tatcaatgtt attcttatta tagttggagg
    19981 ctttgtaggt ttatttttga aaaatttttt gaaagaatca cttcaaaaga gcctcatgca
    20041 agccatggga gtcgcagttt tatttatttc aatatcaggt gttttggaga aaatgatgtt
    20101 agtcgaaaag tcacatctca tctctaatca tacaaatatg atgattatta ctcttgctct
    20161 aggtacggta cttggagagt tgctatcact agattcgtat attgatagat ttggggacta
    20221 tcttaagcaa aaaacaggaa gtggtaacga tattaaattt gtggaagcat tcgtcacctc
    20281 aacttgtact gtttgtattg gagcaatggc cgttgttggt agcattcaag atggtatagc
    20341 agcagatcat tcgattttat ttgctaaggg aatgttagac atgattatta ttgcaattat
    20401 gacagtgtct ttagggaaag gggctctatt ttctgcactt cctgtcgctt tactacaagg
    20461 tagccttacg attgttgctt ttttcatggg gagcctactt aatccatcat ctttagatta
    20521 ccttaactta gtaggaaata tgcttatatt ctgtgtcggt gttaatttac tctttaacct
    20581 aaatattaaa gttatcaata tgttgccagc aattatttta gctatattat ggggaagttt
    20641 tatataaaag ataaccaagt tttaactcaa acttggtttt tctttagaca tatgttagga
    20701 tgaaagaaga tatactacgg agaggatcca actcatgaat ctattatttt ctattgatga
    20761 tatgtacgta gatcatttta aagtgatgct ttattcgctt gtccgacaaa ccaagaatcg
    20821 taagttagag atatatgtct tacaaaaaac attactcaaa cgtcatacag aacttattca
    20881 atatactcaa aatttagaag ttggctatca tccaataatt gtaggtaccg aagtatttgc
    20941 tcaggcacct acaactgatc gctatccaga tacaatatat tatcgacttt tggctcataa
    21001 atttttacct gagaccttag ataggatact ttatttagat gcagatatgc tttgcttaaa
    21061 tgatttctca agtttatatg acatggagtt aggtgatcaa ctatatgcag cggcaagtca
    21121 taatactgat ggtaagtttc tagattatgt caataaatta cggttaaaaa atgtagagct
    21181 agaatcctct tatttcaata caggggtact cttgatgaac ttgcctgcta tacgaaaagt
    21241 tgttcatcaa caaactatct tagattacat aatgcaaaac cgtggtcgtc taatcttacc
    21301 agatcaggat atcttaaatg gcttatacgc taatttggtt aaaccaatac ctgatgaaat
    21361 ttataattat gatgctaggt atagtcttat ttatcaatta aaaagtcgca atgaatggga
    21421 tttagagtgg gttattaacc atactgtttt tttacatttt gcaggacgag ataaaccatg
    21481 gaaaaaagat tatagaggac gttactcggg cctttataag tttatggcta aagaggcttt
    21541 aactaaaaat tgaagattta aaaggttact aaattatttc tatttagtaa cttttttggg
    21601 ggtgatattt taatgatatc aataatcttt gctaataatt atttataaaa aagaaaaata
    21661 atatcaaagg aggagcgtgt taaaatcact taatcattga acaatcaaat taatcattgt
    21721 ttcaaaagca taatggtttt aggagttact caaatgaaaa aggaatttat tattacaggt
    21781 cggatggttg caagagttat ggaaacccta gtttgcttat gtatggtaag taatcttgtt
    21841 cttgatatgc ttgatataat agaaataaat gaaattgtat tttgtgtgtt ggtagcatca
    21901 attgcagggc ttgagttttg tttgcaaaaa gaaagggagt agatagatgg aatttttggc
    21961 ttaatggctt tttatggtca tgacgtatat tggtgtacct aaagagcaaa aggcactcag
    22021 tcgtaggggt aaacaattag aaaaaataaa tcagggagga aaaacactta tgtcacaaat
    22081 gattcaagat ttagtaggaa gcacatgtat gatttattta gaggatggtt ttcaaacgca
    22141 tccttatgag attttaaagg tagatgagga ttgggtacag attcgtcgca acttgaaacg
    22201 aagtaaagag gaggtacgta ttctccgtat tcatgatatc aaggaattgc gtccagtcaa
    22261 taaatagagt gaggaataaa tctatatatc attttataaa ggagaaaaat gatgggatta
    22321 attttaaaaa acaggctcaa agaattgcgt gcacgagatg gtatcaatca aacagaactc
    22381 gccaaattaa cgggagtctc aagacaaaca attagcctaa ttgaacgcgg agaatatacg
    22441 ccatctgtta ttattgctat gaagatttct caagtattta aagaatccct agagaatgtt
    22501 tttagtttag tgtcaacgga ggaataacga tgaaaaattt tataaaacaa agtgatacta
    22561 catttgttcg aattatcaaa agtcttttaa ttggtggttt tataggggcc atacttggtt
    22621 ccgtaggagc tctttttaac atatttggtc aagataagta cttatctgaa attaatattg
    22681 ttcaatattt cctatgggtt agtcgtattg ttgtaattat aacagctctc tttagtctca
    22741 tctacctcta tcagattcaa aaatatcaga aagttttttt caatgttgat gaaagtcaat
    22801 ctgaagaaat ttatcgccag attaatttaa gacattctta tgggatgact tttgtgtcaa
    22861 tttcaatagt actcagtata tttaatactt tatttaacta taaactaaat atttttgatg
    22921 attcagttac tttagttatt ccaatatatg atttatcact gttatttgtt ttattgggtt
    22981 tacatatata ttttttaaag gtatatcgga atatccgagg tataaaaatg acagtcgctc
    23041 caactttaaa agagttgaaa aacaatgtct tacaattgga cgaagcagag ttggaatcaa
    23101 attataagat gtgttttgat attgtcatga atttaagtgg ttttattttc cctacgatct
    23161 attttgtatt attctttatc tcatttgttt ttcaaaaagt ggagattgt
//
LOCUS       DATRSG010000028        23202 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRSG010000028 DATRSG010000000
VERSION     DATRSG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23202
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.028"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            149..1483
                     /locus_tag="VBD89_001076"
     CDS             149..1483
                     /locus_tag="VBD89_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO7135615.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKRITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1551..2339
                     /gene="pflA"
                     /locus_tag="VBD89_001077"
     CDS             1551..2339
                     /gene="pflA"
                     /locus_tag="VBD89_001077"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO7135616.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2456..3391
                     /locus_tag="VBD89_001078"
     CDS             2456..3391
                     /locus_tag="VBD89_001078"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO7135617.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3451..4104
                     /locus_tag="VBD89_001079"
     CDS             3451..4104
                     /locus_tag="VBD89_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO7135618.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(4134..5135)
                     /locus_tag="VBD89_001080"
     CDS             complement(4134..5135)
                     /locus_tag="VBD89_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7135619.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(5132..6157)
                     /locus_tag="VBD89_001081"
     CDS             complement(5132..6157)
                     /locus_tag="VBD89_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7135620.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6173..7105)
                     /locus_tag="VBD89_001082"
     CDS             complement(6173..7105)
                     /locus_tag="VBD89_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7135621.1"
                     /translation="MKKIGIIVLTLLTFFLVSCRQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(7129..7923)
                     /locus_tag="VBD89_001083"
     CDS             complement(7129..7923)
                     /locus_tag="VBD89_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135622.1"
                     /translation="MSHIKSENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(8082..9533)
                     /locus_tag="VBD89_001084"
     CDS             complement(8082..9533)
                     /locus_tag="VBD89_001084"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO7135623.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLK"
     gene            9657..11291
                     /locus_tag="VBD89_001085"
     CDS             9657..11291
                     /locus_tag="VBD89_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7135624.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            11426..12646
                     /gene="pepT"
                     /locus_tag="VBD89_001086"
     CDS             11426..12646
                     /gene="pepT"
                     /locus_tag="VBD89_001086"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO7135625.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            12675..13166
                     /locus_tag="VBD89_001087"
     CDS             12675..13166
                     /locus_tag="VBD89_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO7135626.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(13153..13350)
                     /locus_tag="VBD89_001088"
     CDS             complement(13153..13350)
                     /locus_tag="VBD89_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO7135627.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            13396..13920
                     /locus_tag="VBD89_001089"
     CDS             13396..13920
                     /locus_tag="VBD89_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7135628.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            13931..14614
                     /locus_tag="VBD89_001090"
     CDS             13931..14614
                     /locus_tag="VBD89_001090"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO7135629.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    14635..14760
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14775..15305
                     /gene="infC"
                     /locus_tag="VBD89_001091"
     CDS             14775..15305
                     /gene="infC"
                     /locus_tag="VBD89_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO7135630.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            15345..15545
                     /gene="rpmI"
                     /locus_tag="VBD89_001092"
     CDS             15345..15545
                     /gene="rpmI"
                     /locus_tag="VBD89_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO7135631.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            15603..15962
                     /gene="rplT"
                     /locus_tag="VBD89_001093"
     CDS             15603..15962
                     /gene="rplT"
                     /locus_tag="VBD89_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO7135632.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(16205..18349)
                     /locus_tag="VBD89_001094"
     CDS             complement(16205..18349)
                     /locus_tag="VBD89_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO7135633.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDNRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKTIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            18484..19641
                     /locus_tag="VBD89_001095"
     CDS             18484..19641
                     /locus_tag="VBD89_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO7135634.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            19641..20318
                     /locus_tag="VBD89_001096"
     CDS             19641..20318
                     /locus_tag="VBD89_001096"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO7135635.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            20412..21479
                     /locus_tag="VBD89_001097"
     CDS             20412..21479
                     /locus_tag="VBD89_001097"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO7135636.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            21480..22646
                     /gene="aroC"
                     /locus_tag="VBD89_001098"
     CDS             21480..22646
                     /gene="aroC"
                     /locus_tag="VBD89_001098"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO7135637.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            22730..23065
                     /locus_tag="VBD89_001099"
     CDS             22730..23065
                     /locus_tag="VBD89_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO7135638.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         7580 a         3650 c         4449 g         7523 t
ORIGIN      
        1 tgctataaaa tgccataaac aatattatcg actatgcgct cagtgctatt atatgatata
       61 ataatagcat tatctaatag aagaacagtt ggtagagtta atcgctatca gctaaattca
      121 aataattttt atttaggagt aattttccat ggaagaccct ggcagtcaga gtttgttact
      181 acaatttgtt attttattaa tattaaccct gtttaacgcc ttcttttcag catctgaaat
      241 ggctttagta tcgctaaacc gctctaaagt agaacagaaa gctgaagaag gggacaaaag
      301 gtatcgacgt cttttggacg ttcttgaaaa tcccaataat tttttatcaa caattcaagt
      361 tggtattact tttattagcc ttttgcaagg ggctagttta tctgcctcac taggacatgt
      421 gatttcagga tggctaggaa attcagcaac agcccgtaca gcagggagta ttattgcgct
      481 aatcttttta acatatgtct ctattgttct aggagaatta tatcctaagc gtattgctat
      541 gaatttgaaa gatcgtctag cgattgtttc agctcccatt attatctttc tggggaaaat
      601 agtaagcccc tttgtatggt tgctctcagc ttcaactaac ttattgagta gaatcactcc
      661 gatgactttt gatgatgctg acgagaagat gacacgtgat gaaattgaat atatgttgac
      721 gaatagtgaa gaaactttgg atgctgaaga aattgagatg ctgcaaggga ttttctcgct
      781 agatgaaatg atggcgcgtg aagttatggt tccgcgtact gatgctttca tgattgacat
      841 caacaatgat gcacaatcaa atattgaagg aattttatca caaaactttt ctcgtgtccc
      901 tgtttatgat gacgataaag atagagttgt tggtgtttta cataccaagc gcttattgga
      961 agcgggcttt aaaactggtt ttgatactat cgacttgcga aaaatacttc aggaacctct
     1021 ttttgttcca gaaacgattt ttgtagatga tcttttaaaa gcattacgca acactcaaaa
     1081 tcaaatggcc attttactag atgaatatgg tggcgttgct ggtttagtaa ctctagagga
     1141 tttattggaa gaaattgtcg gcgaaattga tgatgaaaca gatacagctg aacaatttgt
     1201 cagggaaatc gatgagaata tctatattgt tctaggaact atgacgctta atgaatttaa
     1261 tgattatttt gagacagagt tagaaagtga tgatgttgat acaattgcgg gttattattt
     1321 aactggtgtg ggtagtattc caaaccaaga ggaaaaagta gcttacgaag tagacagcaa
     1381 agataaacgc atcactttga ttaatgataa agttaaagat ggtcgtataa caaaattgaa
     1441 agtgctattg tctgatatag aacagaatat tgaaaaagac taagattact tagtcttttt
     1501 ttcatgaaaa tgatataata aagtcataaa aacggttaca aggtgatgaa atggcagaaa
     1561 tagactataa aaaagtaaca ggaatgatcc attctacaga aagtttcggc tctgtggatg
     1621 ggccaggtat tcgttttatc atttttatgc aaggatgtaa aatgcgttgt caatactgtc
     1681 ataatcctga tacttgggaa atggaaacta acaattcaaa ggaacgcacc gttgaggatg
     1741 ttctaaaaga agctcttcgt tataaacatt tttggggaaa agatggaggt atcacagttt
     1801 caggtggtga agcaatgctg cgaattgact ttattacagc tctctttatt gaggctaaga
     1861 aactaggtat tcatacgacg ctagatactt gtggttttgc ttatcgagca acaccagaat
     1921 accatgctat ccttgaaaaa ttgttagatg ttactgattt ggtactcctt gacttaaaag
     1981 aaattgatcc agaacaacat aaaattgtaa ctcgtcagtc taacaaaaat attttacagt
     2041 ttgctagata tttatctgat agaggtactc cggtatggat tcgtcatgtt ttagttcctg
     2101 gattgactga tattgatgac catttaaaac gtttgggtga atttgtccaa acacttgata
     2161 atgtcgataa atttgaagta ttgccttatc atactatggg agagttcaaa tggcgtgaat
     2221 taggaattcc gtatccatta gctggtgtga aaccaccaac tccagaacgt gttaaaaacg
     2281 ctaaagatat catgaaaaca gaatcctata cagaatatct aaaaagaatt caaaattagt
     2341 tttaagttga attttattca acttttttag ttgcaaaggg cttcctagta agattttttg
     2401 ctaagttatt caatttttgc tacaataagg tgaatataaa agaagaggta aaaacatgtc
     2461 taaaatttta gtctttggtc accaaaatcc agattcagat gctattggat catcagttgc
     2521 ttttgcatat ctagctaaag aagcttgggg gcttgacaca gaggccgttg ctttaggaac
     2581 tccaaatgaa gaaacagcat atgttttaga ttattttggt gttcaagcac ctcgagtagt
     2641 agaatctgca aaagcagagg gtgtagagac agtcatttta actgaccata acgaattcca
     2701 acaatctatt tctgatatta aagatgtgac agtttatggt gttgttgatc atcatcgtgt
     2761 tgctaacttt gagactgcca atccgttata tatgcgtttg gaaccagttg gctcagcatc
     2821 atcgattgtt taccgtatgt ttaaggaaaa tggagtatca gtacccaaag agttggctgg
     2881 tttattatta tcaggtttga tttcagatac tcttcttttg aaatcaccaa caactcatgc
     2941 ttcagatatt ccagttgcaa aagaactagc agagcttgca ggtgtaaact tagaagaata
     3001 tggtttagaa atgcttaaag caggtactaa cctctcaagt aagactgcag cggagttaat
     3061 tgatattgat gctaaaacat ttgaattaaa tggagaagct gttcgtgttg cacaagttaa
     3121 tactgtggac attaacgata ttttagcacg tcaagaagag attgaagttg ctattcaaga
     3181 agcaattgtc acagaaggtt attcggattt tgtattgatg attactgaca ttgttaattc
     3241 aaattcagag attttagctc tcggttcaaa tatggctaaa gttgaagcag cttttgaatt
     3301 tacacttgaa aataaccacg cttttcttgc tggggcagta tcacgtaaaa aacaagttgt
     3361 tcctcaattg acagaaagct acaatgctta attaatcatg taaaacgggc tgcgtcccgt
     3421 ttttttagtc atacatttag gagcgttttt atgatattaa cgtttaatcc aggaaagtta
     3481 gaaagacaag agttttttaa ggagcttatt aactatcttt ggatacatga tgatgtaaca
     3541 ctaagacaaa taaagtccca ctttacagac tattctaaaa ttgaccgttt gttagaagaa
     3601 tatattaatc atggatatat ccttcgccaa aataaacgct acagccttaa tcttcctttt
     3661 ctgagttcgc tggatggatt agcattagat gatttggttt tcatagattc tgctagtcaa
     3721 atctatcagt tattacagaa gcgaaaattt gtgacaaatt tagataacca aaccaatcac
     3781 ttggtatttg tagaagagac agattttgaa agaaacactt tgactctttc taattatttc
     3841 tataaactaa ctaactgtta tccactttca agagaacaaa aaaagcttta tcaactttta
     3901 ggagatgtta attcggaata tgctttaaaa tatatgtcaa gctttatatt gaaatttctt
     3961 cgcaaggata gtgttaagca aaaacgtaca gatattttta tccaagcttt agaattactt
     4021 ggatatatta gcttaaacca agatactacc tatcgtttaa atgctaaact tgatgttgaa
     4081 gctttaaaga tttacttaac ataaaaaaag actggaagtt tgtccagtct tttttagtca
     4141 acttttgtca tcaaccagag aaaatagggg gcaccaatta ttgatacaac caatccagtg
     4201 ggtatacctg taccaactaa gaggagacga cctatagtat ctgataccaa taggataata
     4261 gcaccaataa gaagacagct aggtagtata attttatggt tatttcctaa tagtcgtcta
     4321 cttaagtgcc cagcgagtaa gcctataaaa gtaatattac caattaagac gacgctaata
     4381 gcagcaaggc tacttgccag catcaaagta tatattcttt ccttttttaa atgaagccct
     4441 agagcgagag cagtctgctc gtttaggttc ataatattaa ggtggcgact gcgactatat
     4501 gctaatcccc ataacaaaat gagaatcggg ataaaagtga taatggtagt ccaggaacta
     4561 attgtaattt ttccactaag ccattcaacg atcatatcca ttttttgatt attagactga
     4621 ctaataatag aaatcataac gcctgaaatc attgtagata ttccgatacc agtgatgatt
     4681 aatcgtgtag ggctaatatt gtgatttctt ctgtatgaca taagatagac aagaaaaata
     4741 gttaccaacc ctccaaacat agcaaatagg ggtaaaaagt ataatatagt tggattgcta
     4801 acattaaatg ttcctatggt tagagcgatt actaatccag ctccagcatt gataccaaga
     4861 ataccggaat cagctagtgg atttttggtt aatgtctgta atagcaaccc agcaattcct
     4921 aaagaagctc ccccaaagat acagacaata atacgaggta aacgaatgtt agtaatgata
     4981 aaaaggaagg catcatcaga ttttccactt attagtttta aaacgtcaat aacggaagta
     5041 ttagcatagc caagtgaaat tgatactagt attaataata gtaggataat gacagatgat
     5101 attaaaacaa aaggagcttt atttttttga atcatagatt tttccctttt ctaattaacc
     5161 ataggaaaca tggaagccct acgatactga ttatagaact aattggtgtc tctgaagggg
     5221 gatttataat acgagatgat aagtctaccc agatcatgaa agtagcccct gaaaatccta
     5281 ttaaaggtaa taacagacga taatctttgg gaataaaaag cttaataaaa tgtggaataa
     5341 tcaatccaat gaaagaaacg gttcctatca acgcgactga tgaagccgat aaaaacaaaa
     5401 caataagtaa tagaaacgca gtcattaatt gagttttttg tcccaatgct ttggccacag
     5461 attcattaag gctcaagata gttaattgat gtgctaggag ttgtgaaata agtaaaccta
     5521 aaataatgat aggtgctatt atgattagca ttttccagtt aatttgggat aaaccacctg
     5581 cttgccaacc gataacagtt cgattgagtt taaagtataa tgtaaccact tgtccaacag
     5641 acgtaaaaag tgtggagatc atcgctccag ctaaaatgag tcgtagttgg tggtaaccct
     5701 tttgctttgt ataagagagt gtgaagacta aaagacaaga aatgacagac cctagtaagc
     5761 agacaatgag aatcgtacta tagtgcatgc tccctaaaaa agcatatgca acaacaaggg
     5821 ctaacccagc tccggcgtta atacctagta gtccaggatc agcaatggcg tttcgagtga
     5881 ctccctgcat aatagcacct gcttgtgaca tagctgcacc tactaagata gctgcaatta
     5941 tgcgtggtag tctaatgtca aaaataacat cctgagcatt agagtttttt aagggatttt
     6001 ttaaaacagt tattaaatca ctagtcttga aattgatagc accaaatcgt aagctggcgt
     6061 aacaaccaat aagaaatagt ataagtgtta tgaaaaaaac aagccaaaaa tgatttgact
     6121 tatttttttg aacgagtttt tttgaagctt taaacataag aggagctttc ttttaatttg
     6181 tattttcttt gatagccttt gtaaatgatt ttaattgagc ttctaaagat agagggtcag
     6241 agaaataaaa cacgtcgtag ttactttcta tgatgtgccc ttttttgaca gctggtaaat
     6301 tcttccagac atcactttct ttaagtgatg aagctgcttt tttagtcgtt ttgtttatat
     6361 taacaagggc ataatctcca acgtaatcac cgattgcttc ttgcgaaacg gtaaaccacc
     6421 cttttttaaa gacatctttt ttgacttttt ctggggcagc ataacctagt gaatcataga
     6481 ttagttctcc accgcgtcca aaattattac catataaata gatattttta tcataaaaat
     6541 ccataatagt aaaagtagtg ttaggcttta agatatggtg taaatctttt ttggcagcga
     6601 gagttttagt tttccattgg ctaacccact gattagcttc tttttcttta ccgaatactt
     6661 tccccaaggc tggcatcata tctaaataat tttgtgcacc atatttaata actaaagttg
     6721 gtgcaatttt tttcagagta ttgatgtttg gatcttgatc gaaaaccatg attaaatcag
     6781 gtttttgtgc ggcaatagct tctgtatcat cagcagttaa ttttttagct tctttcagtt
     6841 gcttaccaaa aacggggcta tctttttcta agtctaaact gtaacttgaa acattaacac
     6901 ctagttttaa taaataccca gtgtaagaat atgtaaaatt aattactttt ttcggatttt
     6961 caggaatttt tccataatag gtgaagcctt caattttagg cattttagaa atagttgttt
     7021 tagtgctttc ttctttagtt tgttgtctgc aagataccaa aaagaaggtc agtagtgtga
     7081 ggacaataat tccaattttt ttcataagat aacatctcct aaataatatt aattttgatc
     7141 agttgataat tgataagata acaaaacagg ttgtttacta atagggtcct gaatgatatg
     7201 agcatcaatc ttaaaaatat ctttgataat atctttagtc atgatctgac taggactacc
     7261 ttcatagata attttaccgg ttttcattgc aactagataa tcagaatagc gcgcagacag
     7321 gtttaggtcg tgtaggacca taataattgt tttttgcgtt tcgttattaa gttttttgag
     7381 caattctaag acttctaatt gatgattcat gtcaagataa gttgtaggtt cgtctaaaaa
     7441 gatagtatct gtatcttgtg ctaaagccat agcaatccat actttttgcc tctgacctcc
     7501 tgaaaggtca tcaacatctc tattagcaaa ttgcgctacg ttagtcattt ccatcgccca
     7561 atggatttta ctacgatcat catttgtcaa attgccaagg tatttttggt gtgggaaccg
     7621 tccataagat attagttcat aaactgttat cccttcagtt gcttctaaca cctgtgggag
     7681 aagtgcaatc ttcttagcaa tttcctttgt gggtaaatgt gcaatatcat gaccatcaat
     7741 agtgatagta ccatctttta ttttatggat ccgtgtgaga gccttcaaaa gagtagactt
     7801 tccacatcca ttggcaccaa taatcgttgt tattttttgg ttaagtatac tgagggaaag
     7861 attattgatg atttctttct gatcgtaaga tacaataatg ttttctgatt ttatgtgaga
     7921 catagagaac ctttctagca aaaaattaag ttcattataa catagaaaaa aggcttacaa
     7981 aagccttttt tagcattaat taagaaaaat gtgaacgaaa tgataattat ttagaatgat
     8041 tcaaaatagt atcaataatc attattgttt agcttttttt atcatttaag gtattttttc
     8101 gcaacttcta aatctccaga gtaattgtca cgatttcctt tgataatttg ataagcatct
     8161 gcccctttac cagcgataat aattgcatct agtttgcaat tagtaagagt actggcatta
     8221 gcaattgctt cttctcgatc aatgatgata gtaaccggac gattaatatg gcttgcgatt
     8281 tcttgtgaga taactaaggg atcttcaaaa ttaggatcat ctgcagtgag tatcacttgt
     8341 agattaggat gttgattaat gacatctccg aagtctttac gacggctttg acctttatta
     8401 ccaggagctc cgagcactaa tataatatcc cctttttggt gttcctcaac aacagctaat
     8461 aatttcttca aactgtctcc gttgtgggca tagtcaacga aaatctttgc gccgttggtt
     8521 tgtgttagga cttccatgcg gccagggacg gtagtttgtg caattccgtt ttttatatct
     8581 tcaattgaaa caccaagtcg gagacaagca agtccagctg cgagagcatt ttcttgattg
     8641 aattttccaa tcaggttgat attgtaggtg ttctccaact ttcctttgac atggaaagaa
     8701 aaggcctgtg attctgtgat aacattttca gaataatctc catagaaatc atgaggaata
     8761 tattctacct gttctttgac aatattaaaa tgatccatct gactatttac aacgacagca
     8821 ttgctattct ccatcaagag ccgtttgtgg aaaaaataat cttcgaatgt agggtgctcg
     8881 ataggaccaa tatgatcggg actaatattt aagaaaacgc caacgtcaaa ggttaaacca
     8941 taaacacgtt tggttaggta tgcttggctt gatacttcca tgactaagtg tgtcatttga
     9001 ttctcaacgg ctgtagccat catccgaaat aaatcaagac tctcgggggt tgtgagatgt
     9061 gatttaaaga aagatttacc atctaatgtt gtgttcatag ttgagagcat agcaggacga
     9121 tggtttactt ttagcatatg ataagcaaag taagccgcag ttgtttttcc ttttgttcca
     9181 gtaaaagcta gaagtttaag tttgttttgt gggttattat agaaagacat agatatcaaa
     9241 ctcatggctt ttttgatatc attgactaat atcactggaa tatcggcgcc gtaatctatc
     9301 tcagaaacgt aaaaagaaag gcctgctgtg attgcagaat ctagatattc ttttttaaaa
     9361 gtggcaccct tagcaaaaaa taaagtatca gatgaaattt gacgactatc atatgataag
     9421 gcattaaaag ttacattttg tgtccagtta taataataat gctcatgaaa caaaatctct
     9481 ctgaaattat ggtcattttt taaaatttct aaaattttat ctattgtaat catactcctt
     9541 attttagact ttatgttctc gttttacaag tctaaggtaa aagtttataa taaagtgata
     9601 tttctgagat ttttgtcttt taaatgctaa ataatattga aaatagaaag tttaccatgt
     9661 cacaaaaaac aacaaaagtt agtcagcaag aacaaatggt caaagggacg gcttggttaa
     9721 cagcagggaa tttcattagt cgcttgctgg gagccatcta tattatccct tggtatgttt
     9781 ggatgggaaa gcatgctgcc gaagcaaatg ctttatttgg gatgggatat gaaatctatg
     9841 ctttatttct tttgatatca actgttggaa ttccagtagc agttgcaaaa caagtatcta
     9901 agtataacac tttaggtaaa gaagagatga gcatttatct ggttcggaaa atcttgcagt
     9961 ttatgctgat tttaggtgga gtttttgcat taattatgta tattggttca cctctatttg
    10021 cttcacttag taaaggaggg caagaactcg ttccaatatt gcgtagtcta actctagctg
    10081 ttttagtttt tccttcaatg agtgttttac gtggtttttt tcaaggtttt aataatttaa
    10141 aaccgtatgc cattagtcaa gtcgctgagc agattatacg tgtcatatgg atgttattaa
    10201 ctgctttcta tattatgcgt ctaggatcag gagattatat tgctgctgtg acacagtcaa
    10261 cattcgccgc ctttgtcggt atgtttgcta gtatagctgt gctgctttat ttcttatggc
    10321 gttataatat gctatctgcc ttgattagta aaactccaga gcacattaaa ttagatacta
    10381 aagaaatcct aattgagact gttaaggaag caatcccttt tattattact ggtgcagcaa
    10441 ttcaaatttt caaattgatt gatcagtttt catttgggaa tacgatggcg ctctttacga
    10501 attattcaag tgaagaatta cgggttatgt ttgcttactt ttcaagcaac cctggtaaag
    10561 ttacgatgat tctaattgct gttgctaccg caattgctgg agttggtatt ccattgttaa
    10621 cagagaattt tgttaagaat gataaaaaag ccgccgctcg tttggttgtt aataatttac
    10681 aaatgttact aatgttttta ctgccagctg ttgcaggatc cgttatttta gcaaaacctt
    10741 tgtatacagt cttttatggg ttaccacagg gacaagcttt agggcttttt gtcatatctc
    10801 ttattcaaac aattatttta tctatttata ctgttttagc accaatgcta caagcattgt
    10861 ttgaaaatcg taaagctatt atttacttcc tatatggtct ggtggcaaaa gttattttac
    10921 aagttccctc cattttcctt ttccatgcct atggaccact tttctcaacg acagtagctc
    10981 tttgtatccc agtaattctt atgtatttga aaatacatga aattacagga tttaaacgcc
    11041 aagctattcg tcgtacaagt gctttagtcc ttattctaac gctattgatg agctttatta
    11101 tttctatgat aatatggtta atgaacctgg ttattgtacc cgatagtaga ctggtaagtc
    11161 tagtttacat tatcgttatc ggtgcaatag gtttaggggt atatggcttt atggcactgg
    11221 ccacccatct acttgataag atgattggta gtcgtgctca agacttacgc cgaaagttgc
    11281 accttaacta atgaaaaaat aggttagaca gaattgttta acctagtttt ttgacgacca
    11341 aaattatttg tgaaatttta aggtagtcac aatatagtga gcttattttt atgttataat
    11401 taaatggaga aatatgaggt gaaagatgtc ttacgaaaag cttttagaac gatttttaac
    11461 atacgtcaaa ataaatacaa gaagtaatcc taatagtaca caaacgccaa caactcaaag
    11521 tcaagttgac tttgctttaa cagttttaaa accagaaatg gaagcaattg gtttaaaaga
    11581 tgttcattat ttaccttcta atgggtattt ggttggaacc ttacctgcta caagcgaccg
    11641 cttacgccat aaaataggtt ttatatccca tatggataca gctgatttca atgctgaaaa
    11701 tattactcca caaattgttg actataaagg tggagatatt gaacttggag actcaggtta
    11761 cattttaagt ccgaaagatt ttccaaattt aaataattac catgggcaaa cattgattac
    11821 aacagatggt aaaaccttac tgggagcaga cgataagtct ggtatagcag aaatcatgac
    11881 agctatggaa tatttggctt cgcatccaga aattgagcac tgtgaaatta gagttggctt
    11941 tggaccagat gaagaaattg gtataggtgc agataaattt gatgttaaag attttgatgt
    12001 tgattttgcc tatacagtgg atggtggacc actaggagaa ttacagtatg aaacctttag
    12061 tgcagctggt ttggagctta catttgaagg acgaaacgtt caccctggaa ctgcaaaaaa
    12121 tcaaatgatt aatgctttac agcttgctat agattttcat agtcaattac cagaaaatga
    12181 acgtcctgaa caaacagatg gctatcaagg attttatcac ttatatgatt taagtggaac
    12241 agttgatcaa gctaaaagtt catatatcat tcgagatttt gaggaagtta atttcttaaa
    12301 acgtaagcaa ttggctcaag ctatcgctga taatatgaat gaagcattac aatctgaacg
    12361 tgtaaaggtt aaactatacg atcaatatta caacatgaag aaagttattg aaaaagacat
    12421 gacacctatc aacattgcta aagaagtaat ggaagagtta gacatcaagc cgatcataga
    12481 accgattcgt ggtggtacag atggctctaa aatttccttt atgggaatcc ctactcctaa
    12541 tctttttgca ggtggtgaaa acatgcatgg acgctttgaa ttcgttagtc tacaaacaat
    12601 ggaaaaagca gttgatgtta ttttaggcat cgttgctaag gattagtaaa gaaatatcag
    12661 attggagtgg ttaaatgata aaattatttg ggaagatacg ttatcactgg caacctgaac
    12721 tttcttgggc tattatttat tggtcaatag ccattgcacc aatttttatt ggtttatctc
    12781 tgctatatga aagaactgag attccgagtc aggtatttgt tctatttgcc atctttattg
    12841 ttttagtagg cattggattt catcgctatt ttgttattga agaagatgga tatcttagga
    12901 ttgtatcttt taacttttta agacgatcta agtttccaat tgaagatatt gctaaaattg
    12961 aggtcactaa atcaagtgtc actatcaaat ttaataataa ccatgagcgt attttttaca
    13021 tgcgaaaatg gcctaaaaaa tattttttag atgcactagc tattgaacct aaatttaagg
    13081 gagaagtgga gcttctcgat aatttgatta aaatggatta ttttgaatgt tatcgctacg
    13141 ataaaaaagc tcttacaaaa tcgtaagagc tttggtggga catgatttaa ctgctaaaac
    13201 agtatcttga tcagaacttg gaatttcttt ttctaggtta tctgtatcgg aaaatttaac
    13261 gataccatca tcttgataat caaaaatatt tgaatatgtt tggcaaagcc cgcaggcaat
    13321 acatttttct ggaattagtg caactttcat agtattattg taatacttat tgatacgatt
    13381 aacaaggggg aagtcatggc gaaaaaacca tgggaaaaaa aagttgtgga aaacaatagt
    13441 catagaaaag acaaaattac aagaacgagt agaggtgtgg tgtcaagtac tccttggatt
    13501 accgctttct tgagtgcatt ctttgttatt gtcgttgcta ttttatttat agtattttat
    13561 acttcaaata gaggagaaga cagagcaaaa gaaacttctg gtttttatgg tgcttcaagt
    13621 caaaaagtta atagttctaa aacgaaaaaa gcatctacaa gtaagaaaac aacgtcatca
    13681 tcaaaaacta aaactagtga taaatcaaca acatcttcta gtacagtagc aagttcgagt
    13741 gagacatcga catcatcttc agtaaacggt ggaacaatcg tagttcaacc aggtgaggga
    13801 gcagcctcca tagctgctcg tgcaggtatc tcagttgctc gtttacaaga acttaatcct
    13861 ggtcatatga cctcagggta ttggtatgct aatccaggcg atcctgtggt tgtaaattaa
    13921 ggggaggatt atgaactcaa ttaatattgc tattgatgga ccagcatcaa gtgggaaaag
    13981 tactgtcgct aagattattg ctaaaaattt gaattacact taccttgata caggtgctat
    14041 gtatcgttgt gctacttatc tggctttaca gcatggatat gaagcacaag atgtttcgaa
    14101 aattttagga ctcctagctg aaagaccgat ttcttttggt aaagcagaag atggcagcca
    14161 gacagtcttt cttggcacag aagaagttac tttagctatc cgtcaaaacg atgttactaa
    14221 caatgtctcg tgggtttctg ctatcccaga gattcgagaa gagttagtaa accagcagcg
    14281 tcgtatagcc aaagatggtg ctattataat ggatggtcgt gacataggaa cagttgtttt
    14341 gccagatgca gaattgaaga tttttttagt ggcttctgtt gatgaaagag ctgagcgtcg
    14401 ctttaaagaa aatcaagaaa aaggcattga gagtgatttt gaaacactga aatcagaaat
    14461 tgctgctcgt gattataaag atagccatcg agaagtttca ccattaaagg cagcagaaga
    14521 tgctattgaa tttgatacta caggagtatc aattgaaggt gttgtaactt ttattcaaga
    14581 aaaagcagaa aaaataattg acatgaagaa ttagtcatga tatactagtt gcaatgagaa
    14641 aagcagaagt gagaacttct cgccttgcga cttaggttgt ctggcccaac atgtcaagat
    14701 tccatctagg aagattatat gtgcgggctt gatttatcaa gtccgctttc gtttttctct
    14761 aaaaaaataa agaggtgaag atcatagcta aaaaagatct attcattaac gatgaaattc
    14821 gcgttcgtga agttcgtctg gttggtctcg agggtgaaca attaggtatt aaaccattgt
    14881 ctgaagctca agcaattgca gatgatgcta atgttgattt ggttttaatt cagccacaag
    14941 ctacaccacc tgttgcgaaa attatggact atggtaagtt caaatttgag tatcaaaaga
    15001 aacaaaaaga acaacgtaaa aaacaaagcg ttgttactgt taaggaagtt cgtcttagcc
    15061 cagttattga taaaggggat tttgaaacaa aacttcgtaa cggtcgtaag ttccttgaaa
    15121 aaggtaacaa agttaaagtt tctatccgat tcaaaggacg tatgattact cataaagaaa
    15181 ttggagcaaa ggtattggcg gaatttgctg aagcgacaca agatatcgct attattgagc
    15241 aaagagctaa gatggatggt cgccaaatgt ttatgcagct tgcaccaatt ccagacaaga
    15301 aataattgtc gactttatag tattatttaa gaggagaatt taaaatgcca aaacaaaaaa
    15361 cacaccgcgc atcagctaaa cgctttaaac gcacaggttc aggtggattg aaacgcttcc
    15421 acgcgtttac ttcacaccgt ttccacggta aaactaaaaa acaacgtcgt catcttcgta
    15481 aagctactat ggtttcatca ggagatttca aacgtattaa agcaatgctt actcgcctta
    15541 aataataagg tctctgtaaa catttactac atattataaa ggattatttg gaggaaatat
    15601 aaatggctcg tgttaaaggt ggagttgttt cacgtaaacg tcgtaaacgc gtacttaaat
    15661 tagctaaagg ttactatggt gcaaaacata tcttgttccg tactgcaaaa gaacaagtaa
    15721 tgaattctta ctactatgca taccgtgacc gtcgtcagaa aaaacgtgac ttccgtaaat
    15781 tatggatcac tcgtatcaat gcggctgctc gtatgaatgg tttatcatat tcacaattga
    15841 tgcacggttt gaaattagct gaaatcgaag ttaaccgtaa aatgcttgct gatttagcag
    15901 ttaacgatgc agcagctttc acagctcttg cagatgcagc taaagctaaa cttggtaaat
    15961 aagaaataaa aaagttgggt ctgcctaact ttttttaatt actagaaaga aagtacctat
    16021 attataattt aagtgaaaag tttcgttcta tatatgtaga atgaaacttt ttttgtttta
    16081 ttccgatacc tttattattg ttataatttt cctttttgat ttttattgtt ttatagtatt
    16141 attccgtata aaaagcacct caatcgttgg attgaggtgt ggattaaact ttaaatggta
    16201 aagattattt tttagatgtt ttatgtgtct tattaggatc ttgcaattcc aagtaacttg
    16261 gagcgtggaa gagatcaact gttgatttgt tgttatgttt gctgtatatg cttgttgaac
    16321 gatctttcaa tttacgttct ttagctttga gtgctttaag agaatgtgtg taattgaatt
    16381 tttcaacttc tacagttttc aaaccattgt tttcatcaaa tcgtagtaaa tctccagttt
    16441 gaatcgaatc acttgtagat aattgtttat tagtagcatc tcgaatagtt tttatttcag
    16501 ctttagttgt ttcatcagga tttgtgattt cttgtcctga atcagtgtaa tagagatgtc
    16561 cactatagtt agtgtattta ggagtaatat actgaccagt tgttctcata gcgaccattt
    16621 gtttattatc tttagataac aagtcttgtc ctaactgagt atatttagac gtgtctatcc
    16681 ctagaatatg aagtaaggtt ggtaaattat ctacctcacc accgtatgta ttactgataa
    16741 aaccttttga atagccagga atgtggatca tatatggaac acgttgtaac atagcgttgt
    16801 cgtattcaga ccatgtttca gggtctttac ctaataattc agctaagcta ggattgcgtg
    16861 tattagaaat accgtagtga tccccataca ttacaataat agaattatca tacacaccag
    16921 cagcttttag atattcaaaa aatgatttaa gagctgtatc aaggtagttt gctgtggcga
    16981 aataaccgtt aatcgtttca tcgttagtct ttgcaagtgg gaatccctct tcgtcagact
    17041 ctcccttcaa acttgtgtaa gggtaatggt tacttactgt aataaattta gtataaaacg
    17101 gttgttgcat gtgctctaga tatttgatag agtcagcaaa catatattta tcattcaaac
    17161 cgtattggaa tgaattatct ttggtttgtt ttgagaagta gctagagtca aagaagtagt
    17221 cataacccca ctgtttgtaa gcattatttc tattccaaaa tgttccgacg tttccgtgga
    17281 agaccgcact actgtaacct ccattttgag ctaagatatg tggagcagca aattgtgtat
    17341 tttcaccacc ataattaacc atgaatgaac cactacttag accaaaaagt gagttttcca
    17401 tgagtgtctc agcatcggat gtcttaccag ctttgacttg atggaagaag ttagagaagg
    17461 agatagtctc tttcgaatga taaaggctgt taataaatgg agtaaccaca tgttctttac
    17521 catcaacatt taatttatag tcaatcaaga attgttgaaa actctccaaa tgaatcatga
    17581 taacgtttcg cccttttccg ataccataat attctggatt gggtttggca taatgctctg
    17641 ctacatattt tttggcagtc gctaattctt gtgcagtggc accatttctt tctttctgtg
    17701 cttgataagt ttgattacca ctataaatcg aaaatgatgg caagccaaga gctttaacaa
    17761 tataggtgtt tgaaaaacca cgacttagta gttctggacg atcaatttca gctaagaata
    17821 agttaattga aaagagtaaa ccagacaaag ctgtgataga aaaactagca cgtttattaa
    17881 atggacgatt atctaagtgg attttcttgg ttgcaaataa gaaaataagg ataatgaaat
    17941 cgaaaacgta gacaagatcc caaattctca atagattaag agcagagtct ccaaggccag
    18001 ccgaagtttt actactagcc aagacagcgc taactgtaat gaaatcggaa aactctctat
    18061 agtagattgc gtttgcaatt agaaggatat ttacgatagc ataggtgata aaagcggtaa
    18121 tataaaaagc tttggttcgc ttaacatata aggccaaacc tatgattaag agtcctaagg
    18181 ggatggggtt tataatggtc aaaaacaact gataggaatt ttctaaacct aaattaaaat
    18241 cagtatggta agcccaaatt gtctttaacc aatatataac tactaacaat aaaataaagc
    18301 cgagtcgtgt attgatcgca cggctagcaa aatctttaat ttttttcaca gtaggaactt
    18361 cctttaattt ttttcctaaa gataaaatct cagattaaat tataacataa aattttgtaa
    18421 catacggtaa agaaaggtcg aattcagtat gaaaaaagat aaaaatttgt tataatactg
    18481 tttatgaata aacttaaagt gaattctgtc gttgaaagaa aaatcaaatc aggtgctcag
    18541 ttactggaaa aaaaagattt tgataccagt ttagttaacc agttggttca acttttttca
    18601 cagtcaaatc aattcttagg gatggcctat ctttcaccac aaaataaagg gattggttgg
    18661 ttactatcaa gacaaatttt tgattttaac catgattact ttgtatcgct attcgaaaaa
    18721 tctagagaga agcgtcaaaa atttgaaaaa tctagccaaa caacagccta ccgcttgttt
    18781 aatcaggatg gagataactt tggtggccta actatagatt tttatagtga ctatgctctt
    18841 ttttcgtggt acaatgaatt tgtttatact aatcgacaaa tgattgttgc agcctttaag
    18901 caggtctatc ctaatattaa aggggcatat gaaaaaattc gtttcaaagg tttagacttt
    18961 gaaagtgctc atttgtacgg tcaagaggct cctgaatcat ttttgatttt agaaaataat
    19021 atcaaatata gtgtcttttt gaatgatggg ttaatgacag gtattttcct tgaccaacat
    19081 gatgtcagaa aagccttagc aactaatcta tcagaaggta aaaaagttct aaatatgttt
    19141 tcatacactg cggctttttc tgtagctgca gctgttggag gagcattaga gacaacttct
    19201 gttgatttag caaaacgctc gcgtgaactt tcaaaagcac actttgatgc taatcagatt
    19261 gtcacagata accatcgatt tatcgttatg gatgtttttg aatattataa gtatgccaaa
    19321 agaaaacatc tatcatatga tgtgattgtt attgatccac caagttttgc tcgaaataaa
    19381 aaacaaactt tttcggttac taaagattac tataaattaa ttgaacaagc tttagatatt
    19441 ttagcccctg gtggaactat cattgcatca actaacgcag ctaatctaac cgtatcacaa
    19501 tttaagaaac aattggaaaa ggggtttggt aaagcttcac ataattacat tagcttacag
    19561 cagttgcctg aagatttcac tgtaaatgat aaggaccaac aaagtaatta tttaaaagta
    19621 tttacaataa aggtaaaata atgaaaatag tagtaccagt aatgcctcgc agtcttgaag
    19681 aggctcaaga aatagattta tcaaaatttg atagtgttga tattattgaa tggcgagctg
    19741 atgccttacc aaaggatgac attattaatg tagctccagc tatttttgag aaattcgcag
    19801 gtcatgaaat tatttttact cttcgtacaa cgcgtgaagg tggtaatatt gtcttatctg
    19861 atgctgagta tgttgagtta atccagaaaa ttaattctat ctacaatcca gattatattg
    19921 attttgagta tttttcacat aaagaagttt ttcaagaaat gctagaattt ccaaatttag
    19981 tcctgtctta tcacaatttt caagagacac cggagaatat tatggagata ttttcagaat
    20041 taacagccct agcaccacga gttgtgaaaa tcgcagtaat gccaaagaat gaacaagatg
    20101 tcttagacgt tatgaattac actcgcggtt tcaagactat taatcctgat caagtttatg
    20161 cgacggtatc tatgagtaaa attggacgta tttctcgttt tgctggtgat gtaactggat
    20221 ctagttggac atttgcatat ttagattcat ctatcgcacc cggacaaatt actatttcag
    20281 agatgaagcg tgtcaaagca ttgcttgacg ctgactgata cgagataaca aaatgttaaa
    20341 tatactgatg agatccctag caattgctta gggtttttgt cttattaaaa caaacatgag
    20401 aggacattag aatgcaggtt gaagttgact taccgaatca cccttatcat attaaaattg
    20461 aagaaggatg tttttctgag gctggtgact gggtatcaca tttatggcag aaacaaatga
    20521 taactattat tacagatagt aatgtagaaa tattatacgg ggagtcgctt gtcaaccagt
    20581 taaaaaaaca aggttttaca gtacatgttt ttagttttgc tgcgggtgaa gctagtaaaa
    20641 ctttggaagt tgctaatcgt atttatggtt ttttagcaaa gcatcatatg accagaagtg
    20701 atgggatcat tgcacttggt ggaggcgtcg tcggagattt agcagcgttt gttgcttcta
    20761 cttatatgcg gggaatccat tttcttcaaa ttccaactag tttaacagca caggttgatt
    20821 cttccattgg cggaaaaaca ggagtcaaca cttcattcgc taaaaatatg gtaggtactt
    20881 ttgcacaacc tgatggtgta ctgatagatc ctgttactct aaaaacattg ggcaatcgtg
    20941 aattagttga aggtatggga gaagtcatca aatatggctt gattgatgat ataaaactct
    21001 ggcatatctt agaagagatg gatgggtcta ttgatagtat ccttgataat gcattagcta
    21061 ttatttatca ctcttgtcaa gttaaacgaa agcatgtttt agctgatcaa tatgataaag
    21121 ggttaaggat gcatttaaat tttggccaca ctatcggtca tgctatcgaa gtacatgctg
    21181 gctatggaga aataatgcat ggagaagctg ttgcaattgg aatgatccag ctttctcgtg
    21241 tggctgagcg taaaaatctt atgccaagag gtatcagtca agatatttat aatatgtgtt
    21301 taaaatttgg ccttccggtc cactatgcag aatgggataa ggacgtctta tttaacatat
    21361 tgagtcatga taaaaaagca agtggacaat ttattaaaat tgttatactg cctcaattag
    21421 gtagtgcaac agtccaccaa atacctttag aagaaatgag agattatttg gagaaataga
    21481 tgagatattt aactgctggt gagtcacatg gacctagttt aacagctatt atcgaaggga
    21541 ttcctgcggg actgaaatta tctgctaaag atattaatga agatttaaaa cgccgccaag
    21601 gtggttatgg tcgtgggaat cgaatgaaaa ttgaaactga tcaagtgatc attagttctg
    21661 gtgtgcgcca tggaaaaacc ttgggttcac ctattacact gacagtaact aataaggatc
    21721 atagcaaatg gctggacatc atgtcagtcg aagacattga agagcgtcta aagcaaaaac
    21781 gtcgtattaa gcatcctaga cctgggcacg cagatttagt aggtggaatc aaataccgat
    21841 ttgatgattt gagaaatgct ttagagcgtt cttcggctcg tgagaccaca atgcgagtag
    21901 ctattggtgc gattgctaaa cgtatcttga aagaaattgg tatcgaaatt gctaaccata
    21961 ttgtagtttt cggtggcaag gagattactg tccctgataa gctaacagtt caacaaatca
    22021 aagtgttatc tagtcaatcg caagtagcta tcgtcaatcc atcatttgaa caagaaatta
    22081 aggattatat tgacagtgtg aaaaaggctg gagatactat cggaggagtg attgaaacta
    22141 tcgttggagg agttcccgtt ggtttaggct cttatgtaca ctgggataga aagttagatg
    22201 ctaagatagc gcaagcagtt gtatctatca atgcttttaa aggtgttgaa tttggtctag
    22261 gttttaagtc agggttttta aaaggaagtc aagtaatgga ctccatatca tggacaaagg
    22321 accaaggtta catacgtcag tcaaataatt taggcggttt tgaaggcggt atgactaatg
    22381 gagagcctat tgtcgttagg ggagtaatga agccaattcc tacgctttac aagccactaa
    22441 tgtcagttga tattgatact cacgagcctt atagagctac agtggaacgt tctgatccca
    22501 cggcacttcc ggctgcaggt gtcgttatgg aagctgtagt agccaccgtc ctggtgacag
    22561 aagtgctaga aaaattttct tcagacaaca tgtatgaact aaaagaagct gtaaaactat
    22621 atcgcaatta tgttaatcat ttttaagtga aaactagtag cattcaattg caaaaaaagg
    22681 tataaaagtc tacaatagtg gtaagttcaa gtagaaaaag gagtaactta tggctaatgt
    22741 atatgattta gcaaatgaat tagaacgtgc tgttcgtgct ttaccagaat accaagcagt
    22801 tttaactgca aaagcagcta ttgaaaatga tgcggatgca caagtgcttt ggcaagactt
    22861 tttggctacc caatcaaaag ttcaagaaat gatgcaatct ggccaaatgc caagtcaaga
    22921 agaacaagat gaaatgtcta aacttgggga aaaaattgaa tccaatgacc ttttaaaagt
    22981 ttattttgac caacaacaac ggttgtctgt ctatatgtct gatatcgaaa aaattgtctt
    23041 tgcacccatg caggacttga tgtaatagta taaaagagag agctccttga gttttctctt
    23101 ttatgctatt tacattcaga gcatcctaat tgctgcgctt ttacattatt gcattatcct
    23161 ataaaaatgt atttttatac tttttttgag agcggctatt ta
//
LOCUS       DATRSG010000029        21992 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRSG010000029 DATRSG010000000
VERSION     DATRSG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21992
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.029"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            19..591
                     /locus_tag="VBD89_001100"
     CDS             19..591
                     /locus_tag="VBD89_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO7135639.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            867..1718
                     /gene="ylqF"
                     /locus_tag="VBD89_001101"
     CDS             867..1718
                     /gene="ylqF"
                     /locus_tag="VBD89_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO7135640.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            1705..2466
                     /locus_tag="VBD89_001102"
     CDS             1705..2466
                     /locus_tag="VBD89_001102"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO7135641.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            2487..3035
                     /locus_tag="VBD89_001103"
     CDS             2487..3035
                     /locus_tag="VBD89_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO7135642.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
     gene            3275..4237
                     /locus_tag="VBD89_001104"
     CDS             3275..4237
                     /locus_tag="VBD89_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7135643.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            4234..5208
                     /locus_tag="VBD89_001105"
     CDS             4234..5208
                     /locus_tag="VBD89_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7135644.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            5205..5966
                     /locus_tag="VBD89_001106"
     CDS             5205..5966
                     /locus_tag="VBD89_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7135645.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            6028..7056
                     /locus_tag="VBD89_001107"
     CDS             6028..7056
                     /locus_tag="VBD89_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7135646.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            7194..8036
                     /gene="dprA"
                     /locus_tag="VBD89_001108"
     CDS             7194..8036
                     /gene="dprA"
                     /locus_tag="VBD89_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO7135647.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            8131..10251
                     /gene="topA"
                     /locus_tag="VBD89_001109"
     CDS             8131..10251
                     /gene="topA"
                     /locus_tag="VBD89_001109"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO7135648.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            10388..11089
                     /locus_tag="VBD89_001110"
     CDS             10388..11089
                     /locus_tag="VBD89_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135649.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            11101..13731
                     /locus_tag="VBD89_001111"
     CDS             11101..13731
                     /locus_tag="VBD89_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135650.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNSKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNAKVGHYIF
                     MTKQYHRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISVVVIILISLG
                     FIVNHQLKKLNMLDALKSVD"
     gene            13837..14715
                     /locus_tag="VBD89_001112"
     CDS             13837..14715
                     /locus_tag="VBD89_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7135651.1"
                     /translation="MRKSIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            14822..15412
                     /locus_tag="VBD89_001113"
     CDS             14822..15412
                     /locus_tag="VBD89_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7135652.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            15446..16717
                     /locus_tag="VBD89_001114"
     CDS             15446..16717
                     /locus_tag="VBD89_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO7135653.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            16730..17161
                     /locus_tag="VBD89_001115"
     CDS             16730..17161
                     /locus_tag="VBD89_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7135654.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            17253..18137
                     /gene="truB"
                     /locus_tag="VBD89_001116"
     CDS             17253..18137
                     /gene="truB"
                     /locus_tag="VBD89_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO7135655.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            18150..19082
                     /locus_tag="VBD89_001117"
     CDS             18150..19082
                     /locus_tag="VBD89_001117"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO7135656.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            19125..19538
                     /locus_tag="VBD89_001118"
     CDS             19125..19538
                     /locus_tag="VBD89_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO7135657.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            19531..19812
                     /locus_tag="VBD89_001119"
     CDS             19531..19812
                     /locus_tag="VBD89_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO7135658.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            19802..20566
                     /locus_tag="VBD89_001120"
     CDS             19802..20566
                     /locus_tag="VBD89_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO7135659.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            20634..21944
                     /locus_tag="VBD89_001121"
     CDS             20634..21944
                     /locus_tag="VBD89_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO7135660.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
BASE COUNT         7669 a         3588 c         3924 g         6811 t
ORIGIN      
        1 aagagagagg gatagtacat gagaaaacgt tcaattttgc caatattagc tgtcttattt
       61 gttttcatga gtatctttag cgtatactct atttttcatg gtgataaagc taaagaaaaa
      121 gcaagtgtca taaaacaagc atctcaaaca agtcaaactt caaaaaaaga ggtcttacaa
      181 aagaagactt atccaaatct aaataaatat tcaaatctag aaattcatgt ttcatctacg
      241 aggcaaacaa tgacaataac ctcgagtgac aaggtcatct ttaaaacgat tgtttcaaca
      301 ggagcaaaag agagccctac cccaaaagga acttttgtca tagaaccgga acgaggagat
      361 tttttctata acgcctcatc aaaagaagga gcctattatt gggtctcctt taaagagcat
      421 ggtatttatc tctttcatag cgttccgact gatcaacaag gaaatgagat cccagaagaa
      481 gcaaagcaat taggaaaagc cgcctcacat ggctgtgtgc gtatgtcaag ggcggatgca
      541 aaatggttct atgaaaatat tcctcaagga acgactgtta ccatcaaata gtcttaatgt
      601 ggtaaaaatc atgttattaa aagcttggag taaacttcca gagctttttc gtatatccct
      661 taaactcaat tagtaaacga aacctatcag cggaataaat gtataaagta tttcgatatc
      721 attaaaaaaa tgatagaata atagtgttaa tcgaatattt tatcgacctt tatatgcggt
      781 tgaagaagaa taaaactaaa agattagacc tctaacaaat agatgtagac ttttaaggta
      841 gtctatacat aaggagaatt tatttcatgg ctactattca atggttccca ggacacatgt
      901 ccaaagctcg ccgtcaagtt caagaaaata ttaagcatgt tgattttgtt acaattttag
      961 tggatgcaag gttacctctt tctagtcaaa atccaatgct aaccaaaatc gtcggagaca
     1021 aacccaaact aatgatcctc aataaagctg atctagcaga tcccattcga actaaagaat
     1081 ggagagattt ttatgaatct caaggtttaa agacattagc tattaattct aaagaacaat
     1141 caacagttaa aaaagtaacc gacatcgcta aaatattaat gtccgataaa atcgctaaac
     1201 tacgggaacg tggtattcaa aaagagacac ttcgtacgat gattattgga attccgaatg
     1261 caggcaaatc gactctaatg aatcgcctag caggtaaaaa aattgcagtg gttggtaata
     1321 aaccaggtgt cacaaaaggt cagcaatggc tcaaatctaa taaggaactg gaaattttgg
     1381 acactcctgg tatcttgtgg cccaaatttg aagatgaatt agttggatta aagctagcat
     1441 taacaggggc aatcaaagat caattattgc ctatggatga ggtaacaata tttggattga
     1501 attactttaa aacctactat cctgatcgtc tcaaagagcg ttttaaaagt attaatttag
     1561 aggatgaatc tcctgaaatc atcatgacct taacacaaaa attaggttat cgtgacgatt
     1621 acgatcgctt ttataattta tttgtcaaag aagttcgcga tggtaaatta ggacgctata
     1681 cgttagatat cgtcggagaa catgatggca acaattaaag aaatcaaagc aattttagag
     1741 actattgttg atttaaaaga caaacgttgg caagaatatc aaacagattc acgcgcaggt
     1801 gttcaaaaag caatccttca acgaaaaaaa aatattcagt cacatttaga cgaagaagcg
     1861 cgcttagaac aaatgttggt ctacgaaaaa aaactttaca ttgaacatat aaaccttatt
     1921 gcaggaatcg atgaagtagg tagaggtcct ttagcaggac cagtagtagc tgctgctgtt
     1981 attcttccac caaactgtaa aataaaacat ctcaatgact ccaaaaaaat tccaaagaaa
     2041 aagcatcaag aaatctatca aaatatttta gatcaagcgt tagcagttgg tattggtatt
     2101 caagacagcc aatgtattga tgatatcaac atttatgaag ctacaaaaca tgctatgatt
     2161 gatgcagttt ctcatttatc tgtagttcct gaacatcttt taattgatgc tatggttttg
     2221 gatctaccaa ttccacaaac taaaattatc aaaggtgatg ctaattcact ctctattgca
     2281 gcagcttcaa ttgtagctaa agtgaccaga gataaaataa tgtctgatta tgatagcaaa
     2341 tatcctggat ttgcattttc taaaaatgct ggctatggta ctaaagagca tttagagggt
     2401 ttgcaaaaat acggtattac tccgatacat cgtaaaagtt ttgaacctat aaagtcaatg
     2461 ttgtaaataa tacggaagag gaaaagatga ccgaaaaaga aaaaatgtta gcaggacaat
     2521 attatcgtcc atcagcacct gaactacgca aggatagaga agttgcacta aaaaatatgc
     2581 aagcctttaa caatgaagat gatagtagca aacgtaacgt cattttacaa aaatggtttg
     2641 gtgctactgg gaagagtatc catatggaac aacgttttgt ctgcgattat ggttgtaata
     2701 tttatgttgg tgaaaacttc tacgccaatt ttaaccaaac tttcttagat gtttgtgaaa
     2761 ttcgtattgg cgataactgt atgtttggcc caaactgtca actcctcacc ccactccatc
     2821 ctctagatcc aatagaaaga aattcaggat tagaatacgg tgccccaatc caaatcggga
     2881 ataatgtctg gctaggtgga ggagtgacta ttttaccagg tgttgtatta ggagataatg
     2941 tcgtcgttgg cgctggaagt gtcgttacga aatcatttga aaataatgtt gttatagcag
     3001 gaaaccctgc taagataatc aaaaaattag cttaatgtaa gaaaaaacct ttaattataa
     3061 caaaggtttt ttctaattta aaaaatagat aaagagaata ttctgttaat ctaatctatc
     3121 ttatttataa tgattttaaa tagaaaaacc ttgatataat aatgttttct taagtataaa
     3181 attctttgaa aaagtagata tttttccaaa aaattatgtt attatagatt tgtgctcatt
     3241 tttgaacact ttatctttca ttttaaggag ttttatgaaa aagctattaa ccactcctgt
     3301 aatgggttgt actttggtta tactttctct aacgtcccta tttgttggtg ttaaatctat
     3361 cccactggag caaataacac accttaatca atcacaggta gatatttttt tagcgagtcg
     3421 cttaccaaga accattagta tcctaatatc tggtgcaagt ttgtcagtct gtggcctgct
     3481 aatgcaacaa ctcacccaaa acaagttcgt ttcaccgaca acttcaggga ctatggattg
     3541 ggcgaaatta ggagtagttg ttacgctgat tttctttaaa aatacctcta tttttattca
     3601 actatgtatt gcttcaggtt ttgccatttt aggatcgctg ctttttgtaa ctattttaaa
     3661 aatgattact tttaaggata atatttttat acctcttatc ggactgatgc ttggacaaat
     3721 tgtagcagca gcaacagtat ttttaggaac acattttcaa gtactacaat ctgtcaacag
     3781 ttggcttcaa ggtaattttt ccatcatgac tagccatcgc tacgaaattc tttacttagc
     3841 attgccatgt ttgtttttag tgtatttttt tgctcatcag tttaccattg ttgggcttgg
     3901 agaaagcttc gctaaaaatt tagggattgc ttatgaaaag atgatttact ttggtttagt
     3961 tttggtttca attatgacat ccctagtcat tattatcgta ggagctttac ctttcttagg
     4021 tttaatcgta cctaatctaa ttagcttaac taaaggggat catatgtcct caactatctt
     4081 agagacatcg cttttagggg cttgtatcgt catgatatgt gatctttttg gtcgcttagt
     4141 tatttttcct tatgaagtct caataggagt tacgctagga gtattgggta gtgccttttt
     4201 cttgatttca attataagaa atgagggaaa atcatgatta aggatttaga gataccaaaa
     4261 ttacttattt tactgatact actaattgct accattattt tgtttttaat ttatggtatt
     4321 ccaactgacg caaatgaatt tttgattatc tatatcctaa aaacacgtta tcaaaaattg
     4381 attgcattaa ttttggttgg tatctgcatt ggtagttcta gtcttatttt tcaaactcta
     4441 accaataacc gacttttaac tccgtccatt attggtttag atagcttata tgttcttatc
     4501 caaacaggat taatgtattt aatcggagct cagcgtgtca taaaattttc atcttttagc
     4561 agcttcctcc tttcattatt attaatggtt ggattcgctt acttattatt tacgatactt
     4621 tttcgaaata aaaaacagtc tttgtatttt gttctattag ctggactcat atttaataca
     4681 ttattttctt ctatctcatc ctttattcaa gccattatgg accccaacga ttttatgatt
     4741 ttacaaaatc agctctttgc tagtttcaat gctattaata ccaagatcct ttggatttct
     4801 ttcattatca ttgtcgttag ctttgtcatt aattggcctt ttataaagga attggatgtc
     4861 ttactattgg gtaaagaaaa tgcgatatca ctcggcatct catatcaaaa gcttactact
     4921 agaatttttt tatggttagc attgatggta gctatagcaa cagcattagt tgggccgatt
     4981 acttttttag gcttacttgt agcacatatt acctatcaca gttttcatac ctttagacat
     5041 caaatattag ttcctatagc cattgttatc tgtatcttta cacttgtctt agggcagcat
     5101 ctcgttcaaa atctattaca cctcactgtt caactaagtg ttctcctcaa ccttatcggg
     5161 ggaagttatt ttatattcac cttaatcaaa ggaaggaaaa acttatgatt caaataaaca
     5221 atttacataa gttctatggc caaaaggaaa tattaaaaga cattaatatt tcgattccta
     5281 aaggaaaagt aacagctatt ttaggtccaa atggatctgg aaagagtaca ttgctgagtt
     5341 gtatcagtcg acttgaacct tatgataatg gtgaaatttt ccttgataaa gtaccattag
     5401 cccactacag ttcaaatgac ttagccaaaa cattagccat attaagacaa tctaatcact
     5461 taacactcaa gataaaggtg agtgacttga taggattcgg tagatttcct tatagtaaag
     5521 gacggttaag tcaaaaagat aaagctgtca tagaatcagt tatatcttat atggatttaa
     5581 acgatatagc tgacgagttc attaataacc tcagcggagg tcaaattcaa agagctttta
     5641 tcgcaatgac aatggcacaa gatactcaat atatttgctt agatgagcca ctcaacaatt
     5701 tagatatgaa atatgctgtt caaatgatgg atcttatcaa acgatatgcg tatgagttta
     5761 ataaaactat agtgattatt attcacgaca tcaattttgc aactcactac gctgacaatg
     5821 ttgtcgcttt aaaagaagga caagtggtgt cttgtgggac tgtcgaagat gttatgcaag
     5881 aaaaaatcct ttctcattta tttgatatgc ctatccgtat cgaaactgtt gatggtaaac
     5941 caattatcct tttttgtaca acgtaaataa agagatttat gtaggataac atttttagtt
     6001 tttatctatt ttaggagatt ttatttaatg acaaaaaaac ttattattgc tatattagca
     6061 ctatgcacta tcttaaccac ttctcaagct gttttagcta aagaaaaatc acaaactgtt
     6121 accataaaaa acaactattc ggtctatatt aaaaaagaaa aaagagacaa gccggataat
     6181 aaaaagcaaa tcagcgagac acttaaagtt cctttaaaac ccaaaaaagt agttgttttt
     6241 gatatgggag ctttggatac tatcacagct ttaggagctg aaaaatctgt tattggtatc
     6301 ccgaaggcta aaaatgctct aagtttattg cccaataacg tcaaatctgt ttataaagct
     6361 aagagatacc aagacgtagg aagtctcttc gaaccaaact ttgaagctat tgctcgtatg
     6421 caacctgatg tggttttcct aggagcacgt atggcttctg ttgataatat tgaaaaatta
     6481 aaggaggctg cacctaaagc agcattagta tatgctggag tcgactcaaa aaaagtattt
     6541 gacaaaggag ttgctgagcg tgtcacaatg ttagggaaaa tcttcgacca aaataaaaag
     6601 gcaaaaacct ttaataaaga tatcgcacaa gctgttctta aattgcagaa aactattgag
     6661 aaaaaaggta aacctacagc tctatttgta atggcaaaca gcggtgaact tttaactcaa
     6721 tcaccttctg gtcgttttgg ttggattttc tctgtaggtg gatttaaagc agtcaatgaa
     6781 aatgaaaaac taagttcaca tggtactccc gtatcttatg aatacatcgc tgaaaaaaat
     6841 cctaactatc tctttgtttt agatcgtgga gcgactattg gacaaggagc ttcatcaaaa
     6901 gaacttttta ataacgatgt tattaaagca actgatgctg tcaaaaacaa acgtgttcat
     6961 gaggtagatg gaaaagattg gtatatcaat tcaggcggaa gccgagtaac actccgtatg
     7021 attaaagatg tacagaactt tgttgataat cgttaatttt ttgtcaaagg ctaggaaaaa
     7081 tcctagtctt atttttttga aaaaatagct attatcaatg cttcagagta tagattgatc
     7141 ttatcacttt attttgtcta ttattttcga atatagtcaa tggaggtaac cttatgaatc
     7201 attttgaact atttaagctt aaaaaagctg gactaacaaa tcttaatatc aacaatatta
     7261 tcaactatct caaaaagaat agtttaactt ctctatctgt tcgcaatatg gccgtagtat
     7321 caaaatgtaa aaatcctact ttctttatag aaaattataa acagctagac cttaaaaaac
     7381 ttcgacaaga attcaaaaaa tttccagttc tatcgatttt ggattctaac tatcctttag
     7441 agttaaaaga aatatataat ccaccagttc tactttttta tcagggtaat attgaacttc
     7501 tatctaaacc taaattagct gtagtggggg caagacaggc atctcagata ggttgtcagt
     7561 ctgttaaaaa gattatcaag gagactaaca atcaatttgt tatcgtaagt ggtttagcgc
     7621 gtggcattga tacagcagca catgttagtg ctttaaaaaa tggcggcagt agtatagctg
     7681 ttatcgggag tggtttagat gtttattatc caacggagaa taagaaactt caagaatata
     7741 tgtcatataa tcatctcgta ttatcagaat attttaccgg agaacaaccc ttgaaatttc
     7801 atttccccga acgtaaccgt attattgcag ggctgtgtca aggtattgtg gttgccgaag
     7861 ctaagatgag atctggaagt ttaattacct gtgaaagagc attagaagag ggacgagaag
     7921 tttttgccat tcccggaaat attatcgatg gcaaatcaga tggatgccac catcttatcc
     7981 aagaaggagc taaatgcatt atttcaggaa aagatatcct ttctgaatat cagtagtgtt
     8041 ttgctttcta ctataggaga agttttctat tgacagttta agaaaagtga gttattatgc
     8101 tataagttta cgatatagaa agtgatacct atggcaacta cgacaaaaac gtcgaccaaa
     8161 aaaacaagta aaaaaaaatc agctactgct aaaaaaaatc tagttattgt ggagtctcct
     8221 gcaaaagcaa agactatcga aaaatactta ggacgtaact ataaagttgt agcctcagta
     8281 ggtcatatcc gtgatttaaa aaagtcaagt atgtccattg attttgagaa taattacgaa
     8341 ccacaatata ttaatattcg tggtaaagga cctctcatta atgatttaaa aaaagaagct
     8401 aaaaaggcta aaaaagttta cctcgcgagt gacccggacc gtgaaggaga agctatttcc
     8461 tggcatttag cgcatatttt agatttagac aaagaagaca gaaaccgtgt tgttttcaat
     8521 gaaatcacaa aagatgctgt taaaaatgct tttgtggaac ctcgtcagat taacatggat
     8581 ctcgttgatg ctcagcaagc acgacgtgtt ttagatcgca ttgttggtta ttctatctca
     8641 cctattttat ggaaaaaagt aaaaaaaggg ctatcagctg gacgtgtaca atcagttgct
     8701 cttaaactaa tcattgatcg tgaaaatgag attaaggctt tccaaccgga ggagtattgg
     8761 actattgacg gttcctttaa aaaaggaaca cgtaaattca atgccacttt ctacggttta
     8821 gacggaaaga aattcaaatt atcgaataat gaagacgtca aaacagttct aaaacgtatt
     8881 aaaactgatg aattcttagt tgaaaaagtt gaaaaaaaag agcgtcgtcg taatgcacca
     8941 ttaccgtata caacttcttc attgcaacaa gatgcagcta ataaaatcaa ttttcgaact
     9001 cgtaaaacca tgatgattgc gcaacagctg tacgaaggac ttagcttagg cacagcaggt
     9061 catcaaggtc tgattaccta tatgcgtact gattctacac gtattagtcc gttagcacaa
     9121 aatgaagcaa cagaatttat tactaaccgt tttggtgcaa attattctaa acatgggaat
     9181 aaagttaaaa atgcttctgg agcacaagac gctcacgaag ctattcgccc atctagtgtt
     9241 aatcatacac ccgaaagtat tgctaagtat ttagacaaag atcaactaaa actttacact
     9301 cttatctgga accgttttat tgcaagccaa atgacagcag cagtctttga cacaatgaaa
     9361 gttaatttaa cgcaaaatgg tgttactttt attgctaatg gtagccaagt caagtttgat
     9421 ggttacatgg ctgtttataa cgatactgac aaaaataaaa tgttaccaga tatggaggaa
     9481 ggagaaagtg ttaaaaaggt taatacgaat cctgaacaac acttcactca acctcctgca
     9541 aggttttcag aagcaagtct cattaaaaca cttgaagaaa acggtgtagg tcgtccttca
     9601 acttatgccc caacgcttga gactattcaa aaacgttatt atgttaagct tgcagctaaa
     9661 cgcttcgaac caactgaact tggtgaaatt gttaatagcc ttattgtaga atttttccca
     9721 gatattgttg atgttacctt cactgccgaa atggaaggga aactagacga agttgagatt
     9781 ggtaaagagc agtggcaaaa gattatcgat gaattttata aaccatttga aaaagaactt
     9841 gcaaaagcag aaactgaaat ggagaaaatt caaataaagg atgaacctgc tggatttgat
     9901 tgcgagctat gtggatcacc aatggtaata aaactaggac gttatggaaa gttttatgcg
     9961 tgtagcaatt tccctgaatg tcataacaca aaagctatca ctaaggaaat aggtgttatt
    10021 tgtcctatct gtcaaaaagg acaagttatt gagcgaaaaa caaaacgcaa tcgtatcttt
    10081 tatggctgtg accgctaccc agaatgtgag tttacatctt gggacaaacc tattggacga
    10141 acttgcccca aatcaaatga tttcttagtg gagaaaaaag tacgcggtgg tggtaaacaa
    10201 gttgtttgct caaatgaaaa atgcgactac caagaagaga aaattaaata aagtggaatc
    10261 tgagtgttta gctcagattt ttaaaaaaat tattatttta cataacttaa tttatgtatt
    10321 tatttttatg ggagaattaa taaaaaagtg ctataatata ggtgtattca tagtgtgagg
    10381 tgccgttatg tcctatattg aaatgaagca ttcctataag cgttatcaaa caggtgaaac
    10441 cgaaattgtt gctaacaatg atattagttt cagtattgaa agaggtgagt tggtggttat
    10501 tcttggtgca tccggcgctg gcaaatctac tgttctcaat atactaggag ggatggatag
    10561 taatagtgaa ggtgaagttt taattgatgg taagaatatt gctaattata ccatcaggga
    10621 attgactcgt taccgtagat atgatgtcgg gtttgttttt caattctata acttagtccc
    10681 taatctaaca gcactagaga atgtagaatt ggcttcagaa attgttccaa aggcattaga
    10741 tgcccaacag gcattagaaa acgtaggttt gggacaccgt atcaatcatt ttccagcaca
    10801 gctttccggt ggcgaacagc aacgcgttgc aatagcacga gctattgcca aaaaacctaa
    10861 actgttactt tgtgatgaac ctactggtgc tttagattac caaactggaa aacaagtact
    10921 agccatcctg caaaagatgg cgcaatcaaa agaaacaact gtgatcattg ttactcataa
    10981 tactgcctta gcgccaattg caaatcgcgt gatccatatg catgattcaa aaatcagtga
    11041 tattgtaata aacgagaatc caagtgatat tcagaacata gaatattaga ggttatacag
    11101 atgggaaaga cattttggaa agatatctat cgatcaatta cgacttcaaa aggtagattt
    11161 tcatcaatcc tcttacttat gatgttaggc tcatttgctt ttataggact gaaggtctca
    11221 gcacctaata tgcaaagaac agcacaaaat taccttgctc atcatcatgt tatggatatt
    11281 accgttttca attcttgggg acttgacaaa catgatcaaa ctgttttaga aagcttaaaa
    11341 ggatcacaag ttgaattttc ttactttgta gatacaaccc ctcaacagaa tagtaaatct
    11401 tatcgattgt attccaatac taaaacgata tcaacctttg atcttgttaa gggacgtctc
    11461 cctttaaata aatcagaaat tgctctctca tttcaggaac gaaaaaaata tgcaataggt
    11521 gataaaatta attttaaaca agataaaaac aaactgtttt caaatacagg tcctctaact
    11581 attgtaggtt ttgttaattc taccgagatt tggtctaaga caaaccttgg tagttctcaa
    11641 acaggggacg gcgacttaga tagttatggc gtattagata agacagcatt tcactctcca
    11701 gtatatacaa tggcaagggt aacttttaaa gatcttaggt tgattaaccc tttctcaata
    11761 agttacaaag aaaaagtagc gaaataccaa gaaaaggtat cgcgaaaatt aaatattcac
    11821 aataaaataa gatataccaa aacaaaaaaa gagagcctac gtaaaatcga tgaggaggaa
    11881 aaaagcctac taaaagctca aaaacaaatc aatcggttag ataatgatag cctagcgatg
    11941 ccactatctc aaagacaagc tattcaaatg aaaattaaac aagaccgact atctcttttg
    12001 aaacgaacaa aagagcttct aaaattgaga cataacaccc aaataatgga atcgcctcaa
    12061 attattgtct acaaccgtac tacctttcct ggaggacaag gatataacac ttttgactcc
    12121 agtacaaata gcacttctaa aatcagtaat cttttcccca ttattttata tttagtcgca
    12181 gcattagtaa ccttaacaac aatgactagg ttcgtcgagg aagaaagaac caacgcaggc
    12241 atattaaaag cactaggcta tagtgaccgc caagttatct ttaaatttat tatttatggt
    12301 tttatagcag gaacattagg gactactttg ggtatcattg gagggcatta tcttttacct
    12361 cgtattattt ctgatatcat ttccaaagac ctgactattc caaacacaca gtatcatctt
    12421 ttcttaaatt acagtttact agcctttgtt ttttcattat taagcattgt tcttccagtt
    12481 tttgttatca ctcgacgtga gttgaaagaa aaagcagcat ttttattatt accaaaacca
    12541 ccagctaaag gatctaaaat agctttagag tatatcaatt ggatttggaa aaaactgtcc
    12601 ttcacacaaa aagtaactgc tagaaatatt tttcgttaca aacaaaggat gattatgacc
    12661 atatttggag ttgctggatc agtagcgctc ttattttcag gtctaggaat acaatcctcc
    12721 ttaaagcaaa ccgtcaatga acatttcggt cgtattatgc cctatgatat attgctaaca
    12781 tataacacaa atgcttctcc cccaaaaata cttgaactac tttcaaaaga ttcaaaaata
    12841 gacaaatacc aaccaataca ccttgaaaat cttgacgaat ccattcctgg acaaattaat
    12901 aaacagtcaa tttccctatt cataacagac aaaaaacaat tactaccttt tatttattta
    12961 caagaagcaa caactaataa gtcattgcac ttgaatagta aagggattat tatttctaaa
    13021 aaattagctc aattttatca tgttaatacc ggtgatttta tccatttatc tcattcacaa
    13081 acacttcctt ctagaaaatt aaaaataaca ggagttgtca atgcgaaagt tggtcactat
    13141 atttttatga caaaacaata ccatcgaact atttttaaga aagaagctaa agataacgct
    13201 tttttggtta agttaaccaa acataaaatc gcaaataact tagcagaaaa acttttagaa
    13261 attaatggag ttgagtctct tacgcaaaat gctcttcagc tggctagtgt agaagccgtt
    13321 gtgcgttctt tagatggctc tatgactatt ttagttgtcg tatctttatt gttagccatt
    13381 gtaattctct ataatctcac taatattaat ttagcagaac gtaagcggga gctatcaact
    13441 ataaaggtat taggttttta taatgaagaa gtaactttat acatttaccg cgaaaccatt
    13501 attttatcaa ccatcggtgt gattctaggc accattagcg gtacttattt acaccgtcaa
    13561 atgatgttgc taattggttc agatcaaata ctttttggtg aaaaagtatc accaactaca
    13621 tttataatac caataagcgt agtagtcatc attctaataa gtctaggttt tatagttaac
    13681 catcaattaa aaaaactcaa tatgttagat gccttgaaat cagtagatta gtttataaaa
    13741 aagattatct tttctaatct tttcgataac gattgacagc aagtaatctt aattgtataa
    13801 tataacagta taaagttaga taaaagtgag atatttatga gaaagtctat ttttgaggaa
    13861 actgcaaaag gtaaagtatc aaggcatgtc cttttaacta tattattggc tttgatatta
    13921 ctatttacag cagatttcct tacctatatt attattgaat tgattatctc gcttgatttt
    13981 tggaaactag agtgggatag cgatatcgtc cagttaatca ttttactagc aacttcaatt
    14041 agtacggtaa tgttatttat gtgggtgaaa tttaaagaga agcgtccaat tagttctcta
    14101 gggctattta aagaaggagc cctaacagaa ttatctagag gatggttagt cgggagcgtt
    14161 ctctttggag cctgcttaat attaacaata atttcaggct ctggagttat cgaaactgtt
    14221 cgttttacag ctgataaatt gatttggttt cttatttttg ctgttggatg gcaggtacaa
    14281 agcgcatctg ttgaaatatt gtgtcgtgga tggttacttc cggtgagtgc cacaaagtat
    14341 agtaagatag tttctgtcag tatcagttca atctttttcg gtcttctgca tagcgctaat
    14401 aatcatgtaa gccttatctc gattttcaat ctgtgtctct ttggactatt tctatcgctc
    14461 tatgttattt taaaaggtaa catctggggg gcgtgtggta ttcatggcgc ctggaactgt
    14521 gtacaaggga gtgtctttgg aatcgaagtt agtggagagc caatgttgtc taactcccta
    14581 gtccatgtta aaacctatgg tgctgactgg attagcggtg gaaaatttgg tgtggaagga
    14641 agcatgatta ccagtattgt tcttatcgta gcatgttatt ggctttatca aaaatcgaat
    14701 tttccttacg actaatagat ttaatctaaa taagcttctc ttactgaaag cttatttttt
    14761 attttagtat taaaaaacta tgttttgtga tataatattt taaagacgaa aggagtctat
    14821 tgtgccacaa aatccaacaa gtaacccaga gaaaaaaact tatttattat ctgaattggt
    14881 agactgcttt aaaggtaaag ctgtgccttc aaaagcagaa gcaggtgata tccgtattat
    14941 taatttgagt gatatgtctc ctttaggaat tgactaccat aacttaagaa cattccaaga
    15001 tgaacaacga tcgcttttaa aatatctact tcaggaaggc gatgttttaa tcgcttcaaa
    15061 aggaactgtc aagaaagttg ctatttttga agagcaagat taccctgtcg ttgcctctgc
    15121 taatattaca atactaaggc ctacacaaca cattagaggg tactacttaa agcttttttt
    15181 tgattcagaa gaaggtcaac aggcacttga gaatgctaac aaaggaaaag ctgtgatgaa
    15241 tattagcaca aaggaactgt taaatattgc aataccaagt atcccgcttt ttagacagga
    15301 ttatcttatc caacgttata aacaggggct caacgattac aaacgtaaga tagcacgtgc
    15361 agagcaggag tgggaacgta tccaaaatga catcagacaa caacttagct agaaccaaat
    15421 acaaaacatc ataggaagag ttatcatgaa cgaaatcaaa tgccctcatt gtggaacagc
    15481 ttttgccatc aacgagtctg aataccatca attactagaa caaattcgtg gagatgcttt
    15541 tgacaaagaa gtaagtgaac ggttggaaaa agaacgtcta atattagggg agcaagcaaa
    15601 aaatcaatta caggaagttg ttgtagaaaa agacaaggag atagctaaac ttcagtacaa
    15661 agtcaaacaa tttcttatag aaaaagacaa tcttctcaaa gacaatgagt accaactcgc
    15721 tgagcaatta aatcaaaaag acatgatgct tcgcgacctt gaaaaccaaa tcgatagact
    15781 acgtttagag catgaaaata gcttgcaaga ggcgctaaca aaagtcgaac gagaaagaga
    15841 tgcaatacaa aatcagttgc acattcaaga aaaagaaaaa gatttagctt tagcttcagt
    15901 aaaaagtgat tatgaagtac aactaaaggc agccaatgaa caagtagaat tctataaaaa
    15961 cttcaaagct caacagtcta ctaaagcagt aggagaaagt ttagaacatt atgctgaaac
    16021 agaatttaat aaagtgcgac atttggcctt tcctaatgct tattttgaga aggacaatac
    16081 attatcaagt cgtggctcaa agggggactt tatctatcga gaaaaggatg aaaatgacct
    16141 tgagttttta agtatcatgt ttgaaatgaa aaatgagtct gatgatacta tcaagaagca
    16201 taaaaatgaa gattttttca aagaattaga taaagatcgt cgtgaaaaat cttgcgaata
    16261 cgcagtttta gtaactatgc ttgaagcaga caatgactat tataatactg gaattgttga
    16321 tgttagtcac aaatacccta aaatgtacgt tatacgtcca caatttttta tccaattaat
    16381 tggtattcta agaaatgcag cactcaatac cttaaaatat aaacaagagc ttgctttgat
    16441 gaaagaacaa aatattgaca tcacacattt tgaagaagat ttagatattt tcaaaaatgc
    16501 atttgctaaa aattataatt ctgcaagcaa aaatttccag aaagcaatcg atgaaataga
    16561 taaatctatt aaacgtatgg aagctgttaa ggctgcttta acaacgtctg aaaatcaact
    16621 acgtcttgca aataataaat tagacgatgt ttctgtcaag aaattaacaa gaaaaaatcc
    16681 aacaatgaaa gcaaaattcg atgctctaaa agactaagag gttatgatta tgaagattag
    16741 aactgctaca ctagacgata gtgaaaaatt agtccctttg tatcaagaat taggatacgc
    16801 aatatcatta tcagagattc aatctatttt aaaggttatt cttactcatt cagattatgg
    16861 ctttttgata gctgaggata atggcaaact actcgccttc gttggatatc ataaacttta
    16921 cttttttgaa aaaagtggga cttactatcg tattttagca ctagtagtta atgaaaagca
    16981 ccgtcgtaaa ggcatcgcta gtcaacttat taatcacgtt aaacagctag ctaaaacaga
    17041 tggaagtgaa gtactggctt taaatagcag tttaaaagaa tatcgtcagg aagcttatca
    17101 tttttatgaa aacctagggt tcaagaaggt tagtacaggc ttttcatact atttagatta
    17161 ggtataaatc cttatagcac tataccaaaa gagcgtaagt ttttacgctc ctttctttgt
    17221 gttaccgctt atttactgat ataatataaa acatgattac aggaataatt aatttaaaga
    17281 aagaagctgg aatgacatca cacgatgctg tattcaagct tcgaaagata ttacatacaa
    17341 aaaaaatagg acacggcgga actcttgatc cagatgttgt tggggtttta ccaattgcgg
    17401 ttggaaaagc cactcgtgtt attgaatata tgacagaaag tggcaaaatt tatgaaggag
    17461 aaatcacttt aggatatgcc acaagtacag aagattctag tggagaggtc atttcaagaa
    17521 ctccactaac tcaatccgat cttagcgagg atgttgtgga tcatgcgatg aaaagtttta
    17581 ccggtccaat tacacaagtt cctcctatgt attcagcagt taaagtaaat ggtaaaaaac
    17641 tctatgaata tgctcgttca ggagaagaag tagagcgacc taaacgacaa ataacaattt
    17701 ctgaatttag acgcacaagc ccattgtact tcgaaaaagg catctgccgt tttagtttct
    17761 atgtctcatg cagtaaaggt acctatgtta ggacgttagc tgttgatcta ggaataaaac
    17821 taggatatgc tagccatatg tctttcctaa aaagaacctc atctgcagga ttatctatta
    17881 cacaatcatt aacgcttgaa gaaattaatg aaaaatataa gcaagaagat ttttcatttt
    17941 tacttccaat tgaatatggt gttttagatc tcccaaaagt taacctaact gaggaagata
    18001 aagttgaaat ctcatacgga cgtcgtatct tattagaaaa tgaagcagat accttagcag
    18061 ctttttatga aaatagagtt atcgctatac tagaaaaacg tggcaatgaa tttaaaccac
    18121 acaaagtatt gttataaaga aagtcaatca tgaaaatcca ttacattaat gattataaag
    18181 atattcaagc taaagaagat tgcgtcttag ttttgggcta ttttgatggc ttacatctgg
    18241 gacataaagc cctctttgat aaagctaaaa aaatcgcgac cgaaaaaaat ttgaaaattg
    18301 tcgtcttaac gtttaatgag acacctcgtc taaccttcgc tcgcttccaa cctgaattat
    18361 tgctacatct tacctctcca gaaaaacgtt ctgaaaaatt tcaagagtat ggcgtcgatg
    18421 agttatattt aatgaatttt accagtcatt tttcaaaagt atcttctgac ctttttatta
    18481 agaaatatat ttatggtctc agagcaaaag ctgctgttgt cggttttgat tataagttcg
    18541 gtcataatcg taccagtggt gattatctag cgcgtaattt caagggacca gtctatatta
    18601 ttgatgaaat ttctgaaggt ggtgaaaaaa tctcttctac acgtattcgt caattgatta
    18661 cagagggtaa tgttgagaaa gctaaccaac ttttgggtta tgagttttca acctgtggca
    18721 tggtagtaca tggagatgct agaggacgaa ctatagggtt cccaactgct aatctagctc
    18781 ctattaatcg cacttactta cctgcagacg gtgtttatat ttccaatgtt ctgattaatg
    18841 gaaaatatta tagagcaatg acgagcattg gaaaaaatat cacttttggt ggtacagaac
    18901 ttcgtttaga agctaatatc tttgattttg atggcgacat ttatggagaa actattgaaa
    18961 ttttctggtt aaaaagaatc agagaaatgg taaagtttaa tggtattgac gatcttgtaa
    19021 aacaacttaa aaaagataaa gaaattgctt taaattggaa aaaagatagt caaactcttt
    19081 aattttttgt ataatagtct ctagtaaata caagaggcgg ttatatgatt accttatttt
    19141 tatcacccag ctgtactagt tgtcgaaaag cgcgtgcttg gctaagtaag catgaggttg
    19201 cttttgaaga gcataacatt attacaagtc cactcaataa agaagaacta ttacaaattc
    19261 tatcgtttac agaaaatggt acagaagaca ttatttccac acgttcaaaa gtctttcaaa
    19321 aacttgctat tgatgtagac gaattatcaa cgtcttcatt aatggaatta atttcagaaa
    19381 atccaagtct tttacgacgc ccaattattt tagacaaaaa acgtatgcag attggtttta
    19441 atgaggatga aattcgtgcc ttcttaccgc gtgactatcg taaacaagaa ctgaaacagg
    19501 ccactattcg tgctgaaatt gagggaaaac atgattagta gtaattatag ttatcctctt
    19561 gatccttcat ggaatacgga agatattact aaggtacttc gctttttaaa tcaagttgaa
    19621 cacgcctatg aaaatagtgt taaagttgat gatttgctag atagctataa agaatttaaa
    19681 aaatttgtta aatccaaggc gcaggaaaaa caaattgacc gtgaattcca gagaactagc
    19741 ggttattcca cttatcaagc tgttaaagca gcacaacaac aagcgaaagg atttatttca
    19801 cttggacgct aaatttgatt tcgctaaaca attagtgtat aaagctggtc aattcattaa
    19861 atctgagatg caaaatactt ttgatgttga ggaaaagtct cgatttgatg atttagtgac
    19921 aagtcttgat aaaaaaacac aaaaattatt aattcaagaa attattcaac attacccaga
    19981 tgataatata cttgccgaag aagatgatgt gcgctcaccc attgcacaag gtaatgtttg
    20041 ggttttagac cctattgatg gaactgttaa ttttattgtt caaaaagata actttgctgt
    20101 tatgttagct tattacgaag aaggcgttgg acaatttggt atcatctacg acgttatggc
    20161 tgatattctt tacagcgggg gaggacattt tgatgtctat gctaacgata aaaaaattgt
    20221 tccttttcaa gagtgtcctt tagaacgttg cttacttgga gtaaattctg ctatgtatgc
    20281 agaaaatgat tgcggtattg cccatttagc ttctgaaacc ttaggtgttc gtatctatgg
    20341 tggtgctgga attagcatgg caaaagtcat gcagggcaaa ttattagctt atttttcgta
    20401 tattcaacct tgggattatg ctgcagcaaa aatcatgggg gagacattag gatttacctt
    20461 attaacgctt gacggggaag aacccaacta ttcaacaaga caaaaagtaa tgttcctacc
    20521 aaagtcgaaa ttaaatctta tccaatccta ccttaccaaa aaataaactt aaaaccttct
    20581 aattaaatag aaggtttttt tgcacgcccg tgattatgta gtataataga aacatgaaat
    20641 taccgaatga atttattgaa aaatatcaga ctatacttaa agatgaagca gaagcttttt
    20701 ttgacagttt tgaacaaaaa cccatatcgg catatcgcac caatcctctc aaagaaaagc
    20761 aacttgattt tccaaatgcc atcccaagta ccccttgggg gcattatggg aaaatatcag
    20821 gaaaatcaat agaacacaca actggccttg tttattctca agaacctgct gctcagatag
    20881 tggctcagat tgctgaacca caagaaggaa tgaaagtgct agatttagca gctgcacctg
    20941 gtggaaaaac aacacacctt ctatcatatt taaacaatac tggtctatta gttagtaatg
    21001 aaatttcaaa taaacgtagt aaaattttag tcgaaaatgt cgaacgtttt ggtgctagaa
    21061 atgttatcgt gactaatgaa agctcccaac gactagccaa atgttttaat tctttttttg
    21121 atcttattgt ttttgacggt ccgtgttctg gtgagggaat gttccgcaaa gatcctcaag
    21181 caatacaata ttggcacaaa gattacccca ctgaatgtgc acagttacaa agagatattt
    21241 taaaagaagc aatcaaaatg ctagctcatg gtggtatact tgtatactct acttgtacat
    21301 ggtcaccaga agagaacgaa gaagtggtta attggctcct tcaagagtat gattacttag
    21361 aacttgttga tattcctaaa ctaaatggga tggttgaagg gattaatgta ccacaagttg
    21421 caaggatgta ccctcatcat tttcaaggtg aaggacaatt cgttgcaaaa ctgagagata
    21481 ctagatctaa ggaagcacag aaaattaagc caaaagcaca gaaaataaat aaaatgcagt
    21541 tacaattgtg gcaacaattt gcacaagacc atttaaagat agacttgaac ggtgttttag
    21601 atgttttcgg cgaccaactc tatcttctac ctaatggtct ccctgatttg tctaaattaa
    21661 aaatagcacg caatggtctc catctaggca ctttcaagaa gaatcgtttt gagccatcgt
    21721 ttgctttagg aatggcactt agcgaacatg acctagtaca gtctattgaa attgatatag
    21781 aacagtttga ggtgtacgta tctggaaatg tagtcaaact agacaagact gttccaaacg
    21841 gttggtatca aattcttgta aaaggcaatg gattaggttt tgcaaaagtg acaaataata
    21901 ctcttaaaaa ttattaccct aaaggactac gatttcagac ataactgtta ttgaatactt
    21961 ttaataaaaa aggaaaaaga aatacttttc aa
//
LOCUS       DATRSG010000030        21657 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRSG010000030 DATRSG010000000
VERSION     DATRSG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21657
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.030"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..506
                     /locus_tag="VBD89_001122"
     CDS             <1..506
                     /locus_tag="VBD89_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135661.1"
                     /translation="LKFFSLFTNVKGISTSVKRRSFLDVILKLISKTPSRLWTNLFVR
                     AFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFDYQY
                     MNQLYPVRLNAKASQLKGFLRVLSYAVTVIDIILIRELKPVILLIVLMLIVTEYYIPY
                     KIKKMID"
     gene            564..1364
                     /locus_tag="VBD89_001123"
     CDS             564..1364
                     /locus_tag="VBD89_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO7135662.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKYNAS"
     gene            1354..1989
                     /gene="trmB"
                     /locus_tag="VBD89_001124"
     CDS             1354..1989
                     /gene="trmB"
                     /locus_tag="VBD89_001124"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO7135663.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            2033..2120
                     /locus_tag="VBD89_001125"
     tRNA            2033..2120
                     /locus_tag="VBD89_001125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2067..2069,aa:Ser,seq:gga)
     gene            2478..2954
                     /gene="rimP"
                     /locus_tag="VBD89_001126"
     CDS             2478..2954
                     /gene="rimP"
                     /locus_tag="VBD89_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO7135664.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            2981..4141
                     /gene="nusA"
                     /locus_tag="VBD89_001127"
     CDS             2981..4141
                     /gene="nusA"
                     /locus_tag="VBD89_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO7135665.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            4163..4459
                     /locus_tag="VBD89_001128"
     CDS             4163..4459
                     /locus_tag="VBD89_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO7135666.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            4452..4754
                     /locus_tag="VBD89_001129"
     CDS             4452..4754
                     /locus_tag="VBD89_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO7135667.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            4774..7557
                     /gene="infB"
                     /locus_tag="VBD89_001130"
     CDS             4774..7557
                     /gene="infB"
                     /locus_tag="VBD89_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO7135668.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADYKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            7647..8015
                     /gene="rbfA"
                     /locus_tag="VBD89_001131"
     CDS             7647..8015
                     /gene="rbfA"
                     /locus_tag="VBD89_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO7135669.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(8087..9091)
                     /locus_tag="VBD89_001132"
     CDS             complement(8087..9091)
                     /locus_tag="VBD89_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7135670.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            9254..9670
                     /locus_tag="VBD89_001133"
     CDS             9254..9670
                     /locus_tag="VBD89_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO7135671.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSSLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            9683..11917
                     /locus_tag="VBD89_001134"
     CDS             9683..11917
                     /locus_tag="VBD89_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO7135672.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            11958..12164
                     /locus_tag="VBD89_001135"
     CDS             11958..12164
                     /locus_tag="VBD89_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO7135673.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            12274..12888
                     /locus_tag="VBD89_001136"
     CDS             12274..12888
                     /locus_tag="VBD89_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO7135674.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            12903..13715
                     /locus_tag="VBD89_001137"
     CDS             12903..13715
                     /locus_tag="VBD89_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7135675.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            13828..16470
                     /gene="polA"
                     /locus_tag="VBD89_001138"
     CDS             13828..16470
                     /gene="polA"
                     /locus_tag="VBD89_001138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO7135676.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANVMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            16500..16940
                     /locus_tag="VBD89_001139"
     CDS             16500..16940
                     /locus_tag="VBD89_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO7135677.1"
                     /translation="MVYHFQNLSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            17022..17501
                     /gene="perR"
                     /locus_tag="VBD89_001140"
     CDS             17022..17501
                     /gene="perR"
                     /locus_tag="VBD89_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO7135678.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            17654..19219
                     /locus_tag="VBD89_001141"
     CDS             17654..19219
                     /locus_tag="VBD89_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO7135679.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKW
                     SDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAGKKGQALLDQIKANGSHTYQATITI
                     YGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTG
                     MMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFAT
                     GLTALGLKKSKQR"
     gene            19332..20018
                     /locus_tag="VBD89_001142"
     CDS             19332..20018
                     /locus_tag="VBD89_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7135680.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            20020..21057
                     /locus_tag="VBD89_001143"
     CDS             20020..21057
                     /locus_tag="VBD89_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7135681.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(21071..>21657)
                     /locus_tag="VBD89_001144"
     CDS             complement(21071..>21657)
                     /locus_tag="VBD89_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO7135682.1"
                     /translation="VPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFSDNTYVFSSHI
                     FWKLLSVLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLIVVSSLITTKA
                     VIKLVQQYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGW
                     HFLTIFSFGLVYIYLLPYQTTARLIFYRNITKNS"
BASE COUNT         7381 a         3327 c         4408 g         6541 t
ORIGIN      
        1 tattaaaatt tttctctttg tttacaaatg ttaagggaat ttcgacaagt gtaaaacgtc
       61 gttcattttt agatgttatt ttaaaactta tttctaaaac gccatcgcgt ttatggacta
      121 acttatttgt gagggcattt ctaagaagct cagattatct tggtttaacc ataaggttgg
      181 taactttaaa tatcttatca gtcatctttg ttaacgaaac ctacctcgcc cttgctctgg
      241 catttgtatt taactactta ttattgttcc aattattagc tttaggtcac cattttgatt
      301 atcaatacat gaatcagctc tatccagtta gattaaatgc taaagcaagt cagctaaaag
      361 gttttctcag agtgttatca tatgctgtga cagtaattga tatcatactt ataagagaat
      421 tgaaacctgt tatattattg atagttttga tgttaattgt gacggaatat tacatccctt
      481 acaaaataaa gaagatgatt gactaaagcc tataaaacaa gtaaaataag aggaaacgac
      541 taaatgaaaa ggagggactg ctggtgacaa tatcaaatca agaattgacg cttactccct
      601 taagaggaaa aagtggcaaa gcctatattg gtacttatcc aaatggtgaa cgtgtctttg
      661 tcaaatataa tacgacacct attttgccag ccttagctaa ggaacagatt gcgccacaac
      721 tactgtgggc acgccgtaca agtaatggcg atatgatgag tgctcaagaa tggttagatg
      781 gtcgaacatt gactaaagaa gatatgggta gtaaacagat tattcatatt ttactgcgcc
      841 tgcacaagtc tcgtccctta gtaaatcaac tgttacaatt agggtataaa attgaaaacc
      901 cttatgattt gttgatggat tgggaaaaac aaactcctat tcaaatccga gaaaacactt
      961 atttgcaatc tattgtgact gagttaaagc gtagtttacc tgagtttcga acagaggttg
     1021 caacaatcgt tcacggagat atcaaacata gcaactgggt tattacaaca agtggtttaa
     1081 tttatttggt tgattgggat tctgttcgtt taacagatcg catgtatgac gttgcatata
     1141 ttttaagtca ttatattcca caaaaacact ggaaagattg gctgtcttac tacggttata
     1201 aagataatga gaaagtctgg agcaaaatta tttggtatgg gcaattttca tatttatcac
     1261 agataatcaa atgttttgat aaacgagata tggagcatgt caatcaagaa atttatgaat
     1321 tacgaaagtt tagagagtta attaaaaagt ataatgcgag ttagaaaacg taagggtgca
     1381 gaggaacact tagaaaataa tccgcactat gttatttcaa atccagaaga agccaaaggg
     1441 cgttggcatg agatttttgg aaataataac cccattcaca tagaagttgg ttccggaaaa
     1501 ggggctttta tcactggtat ggcagagcaa aatccagata tcaattatat tggaattgat
     1561 attcaattgt cagtgctttc ctatgctctg gataaagttc tagatagcgg tgctaagaat
     1621 atcaaattac tattagttga tggttctagt ctatcaaact actttgatac tggcgaagtt
     1681 gatttgatgt atcttaactt tagtgatcca tggcctaaga aaaagcatga gaaacgtcga
     1741 ttgacctata agactttctt ggatacctat aaagacatac ttcctgagca gggggaaatt
     1801 cattttaaaa cagataatag aggtttattt gaatatagcc ttgctagctt ttctcaatac
     1861 ggtatgactt taaaacaagt ttggttagat ttacatgcaa gtgattacca gcaaaatatc
     1921 atgactgagt acgaacgcaa attttcaaat aaaggacagg tcatctatcg tgtagaagca
     1981 cgattttaga ataagtaaat agtaagtggg tagttatttg ctactccttt atggagaggt
     2041 cgcatagtgg ctgagtgcgc acgcttggaa agcgtgtagt ccctaatggg ggctcggggg
     2101 ttcgaatccc ctcctctcct ttaatcttgt taatgtatca gtgtttatta agtgttcacc
     2161 tttttcactc gtcaaaagtc caagtttcga aaaatttctt ttattttatc atcgtttctc
     2221 tttgagcttg aagtattagc ataaagtttt ctaagctctt acaaagaatt gaggacaaat
     2281 tttaattttt tgatataaga ttagtgtacg gtgatgctaa tttaaagtaa atgattgcaa
     2341 taatcgtatt ttgtgctata atagtattaa ataatacaga aaggggcgga gttttacttc
     2401 gcctcttatg tgtgtgattc atagaattta tagtcattgt aagttctaaa gcaagttaga
     2461 aagaagaggt agctgttatc gcaaatcaaa ccattgttga tattgttact caagtcgtaa
     2521 ctcctgctat tcaagcaccg tttgaattag tagatgtaga atacgaaaaa atgggtggcg
     2581 actatgtgct cagtatttta attgataaac ccggaggcat tacggttgaa gatacagcac
     2641 aattgacaga cgttgtaagt cccttacttg acacaattca accagatcca tttccagaac
     2701 agtatatgct tgaagtctca agtcctgggt tggaaaggcc acttaaaaca gcagaagcat
     2761 tatcaaatgc tgttgggtct tatatcaatg taagtctgta taaatccatt gataaagtta
     2821 aaatatttga aggtgactta ttatcatttg atggtgaaac cttgacaatt gattatatgg
     2881 acaaaacacg tcataaaact gtagatattc cctatcagac ggttgcaaaa gcccgtcttg
     2941 cggttaaact ttaagaaagg atgattcttg cgaagcaaga atggacacta tgagcaaaga
     3001 aatgctagaa gccttccgta ttttggaaga agaaaaacac attaataaag aagatattat
     3061 tgatgcagtt actgagtcac taaaatctgc ctataagcgt cgttatggtc aatcagaatc
     3121 atgcgttatt gaatttaacg agaagaaagc agactttacc gtctatactg ttcgtgaagt
     3181 tgttgatgaa gtttttgata gccgtttgga aattagccta aaagacgcac ttgctattag
     3241 ctcagcgtat gaacttggtg ataagattcg ctttgaggaa tctgttacag aatttggacg
     3301 cgtggcagct cagtcagcaa aacaaactat tatggaaaaa atgcgtcgtc aaatgcgtga
     3361 agtaaccttc aatgaataca aacaacacga aggtgaaatt atgactggta ctgttgaacg
     3421 atttgaccaa cgttttatct atgtcaatct cggttcactc gaggcgcaat tatcgcatca
     3481 agatcagatt cctggggaga gctttaaatc tcacgatatg attgacgttt atgtctataa
     3541 agtggagaat aatcctaaag gtgtcaacgt ttttgttagc cgtagtcatc cagagtttat
     3601 caaacgcatt atggagcgag aaattccaga agtatttgat ggaactgttg agattatgag
     3661 cgtctctcgt gaggctggag atcgtactaa agttgctgta cgcagtcata attcaaacgt
     3721 tgatgcgatt ggaacaattg ttggacgcgg tggaagtaac attaaaaaag ttatcagtaa
     3781 tttccatcct aaacgcgttg atgctaaaac aggattggag atcccagttg aagaaaacat
     3841 tgatgttatt caatgggttg aagatccagc ggaattcatt tacaatgcta ttgcaccagc
     3901 cgaagttgat atggtacttt ttgatgatga agatacaaaa cgtgctactg tagttgttcc
     3961 agatagcaag ttatcgctag ctattggtcg ccgaggacaa aatgtgcgtt tagcagctca
     4021 tttgactggc tatcgtattg atattaaatc tgcaagtgaa tacgaaaaaa tggaagcgca
     4081 agaacttcaa actgaggaag tagctcaaga atctgaagtt atttcagata aggcagaata
     4141 atataaatga gagaggtgtt ttatggctaa aacaaaaaaa atacctttaa gaaaatcagt
     4201 tgtttctggt gaagtgatcg ataagcgaga tcttttacga attgttaaaa ataaagaagg
     4261 acaggttttt atagatccta ccggaaagca aaatggtcga ggtgcctaca taaaattgga
     4321 caatgatgaa gctatcctag cgaaaaagaa acgagtgttt gatcgcagtt tttcaatgga
     4381 agtttctgat gaattctatg atgaactatt ggcatatgtc gaccataagg tcaaaagaag
     4441 agagttaggc cttgaataat tcagaaaaag tattaaatct cattgggtta gcacagcgtg
     4501 cggggcgcct tattacaggt gaagaattgg ttattaaagc aattcaaaat caacaagtat
     4561 cgctaatctt tctagcaaat gatgctggcc caaatttgac gaaaaaagta actgataaaa
     4621 gtaattacta caaaacagaa gtctccacag tgttttcaac actggaatta agtgatgcgt
     4681 taggcaaacc aagaaaagtg gttgctgtcg cagacgctgg attttcaaag aaaatgagga
     4741 ctcttatggc ataacagaat aggaggacat caattgtcaa agaaaagatt acacgaaatc
     4801 gctaaggaaa ttggaaaaac gagtaaggaa gtggttgagc aagctcaatc gcttggctta
     4861 cctgtaaaaa gtcatgcttc tagcgttgaa gaaaatgatg ctacacgtat tgttgagagt
     4921 tttagctctt ctaaaactaa agctccaaca aattctgttc aaactaatca aggtgtgaaa
     4981 acagaatcta aaactgttga aaccaaacaa ggcttgtccg atgataaacc ttcaactcaa
     5041 cctgtagcta aaccgaaacc acaaagtcgt aactttaagg cggagcgcga ggctcgtgct
     5101 aaagctgaag cggagaagcg tcaacataat ggggatcacc gtaagaataa tcgccataat
     5161 gatacacgct ctgacgacag acgccatcaa ggtcaaaagc gttcaaatgg taatcgtaat
     5221 gataaccgac aaggtcagca aaataaccgt aataaaaatg atgggcgtta tgctgattat
     5281 aaacagaaac cccaaacgcg tcctcagcaa cctgcaggca atcgcattga ctttaaagct
     5341 cgagcagcag ctttgaaagc tgagcaaaat gctgaatact cacgtcatag tgagcagcgt
     5401 ttccgtgaag agcaagaagc caaacgtcaa gcagctaagg agcaagaact agctaaagca
     5461 gcagcgttaa aagcgcaaga agaagctcaa aaagctaagg aaaaacttgc ttctaaacct
     5521 gttgctaagg taaaagaaat tgtcaataaa gtagccgcta cgccatcaca gacagcagat
     5581 agtcgtcgta aaaaacaaac tcgttctgat aagtctcgtc aattttcaaa tgaaaatgaa
     5641 gatggtcaaa agcaaacaaa aaataagaaa aactggaata atcaaaacca agtgagaaat
     5701 caaagaaata gtaactggaa tcataataag aaaaataaaa aaggtaagac taatggagct
     5761 ccaaaaccag taacagagcg taaattccat gaattaccaa aagaatttga atatactgag
     5821 ggcatgaccg ttgcggaaat tgcaaaacgt attaaacgcg aaccagcaga aattgttaaa
     5881 aaactcttta tgatgggagt tatggcaacg cagaatcaat cattagatgg agacactatt
     5941 gagctattga tggttgacta cgggattgaa gcacatgcta aggttgaagt tgatgaagct
     6001 gatattgaac gcttctttgc agatgaggat taccttaatc ctgataactt aacggaacgt
     6061 ccgcctgttg ttactatcat gggacacgtt gaccatggta aaacaactct tcttgatact
     6121 ctacgtaatt cacgtgtcgc tactggcgaa gctggtggaa ttactcaaca tattggtgct
     6181 tatcagattg aagaagctgg caagaaaatt actttcttgg atacaccagg gcatgcggcc
     6241 tttacttcta tgcgtgcgcg tggtgcatca gtaactgata ttactatcct tatcgttgcc
     6301 gctgatgatg gtgtcatgcc gcagacagtt gaggcaatta atcactctaa agctgcgggt
     6361 gtcccaatta ttgttgctat caacaaaatt gataaacctg gtgcaaatcc agaacgcgtt
     6421 atcagtgaat tagctgagca tggtgtcatc tctactgcat ggggtggtga gtctgaattt
     6481 gttgaaattt cagctaagtt tggtaaaaat attcaagaat tacttgagac agttctttta
     6541 gttgctgaaa tggaagaatt aaaagcagat gcagatgttc gtgctattgg tactgttatt
     6601 gaagcccgtc ttgataaagg taagggagcc gttgcaacgc ttcttgtcca acaaggtact
     6661 ttaaatgtac aagatccaat cgttgttggt aatacatttg gacgtgttcg tgcgatgact
     6721 aatgatcttg ggcgtcgtgt caaagttgcg ggtccatcta caccagtttc tattactggt
     6781 ttgaacgaag ctccaatggc tggtgatcat tttgctgttt atgcagatga gaaagctgcg
     6841 cgtgctgcag gtgaagaacg tgcaaaacgt gctctcttaa aacaacgcca aaacacgcaa
     6901 cgtgttagcc tcgagaactt atttgatacc cttaaagctg gtgaagttaa gtcagttaat
     6961 gttattatca aagctgatgt gcaaggttct gtagaagcac ttgcagcttc tctccttaag
     7021 attgatgttg aaggagtaaa agttaatgtt gttcactcag cagttggtgc tattaatgag
     7081 tctgatgtaa ctcttgctga agcatcaaat gctgttatta taggatttaa cgtacgccct
     7141 actccacaag ctcgtcaaca agccgatgct gatgatgtcg aaattcgtca acatagcatt
     7201 atctataaag ttatcgaaga agttgaagaa gcgatgaaag gtaaacttga tcctgaatac
     7261 caagaaaaaa tccttggtga agccattatt cgtgagacct ttaaagtttc aaaagtggga
     7321 actattggtg gatttatggt tatcaatggt aaagttactc gtgattcaag tgttcgtgtt
     7381 atccgtgatg gtgttgtcat ttttgatggt aaattagcaa gtcttaaaca ctataaagat
     7441 gatgttaaag aagtcggcaa tgctcaagaa ggtggtctaa tgattgagaa ttataatgat
     7501 ctcaaagaag atgacactat tgaagcatac attatggaag aaattaaacg taaataaccc
     7561 ctatgagaaa cccaacttgt atctttagtt ggtagggaat acaaaattat ggctaggctc
     7621 gtcctagctt tcaagttatg agcacaatga agaaaggatt aattatggct aatcatcgta
     7681 ttgatcgcgt tggaatggaa attaaacgtg aagtcaatga gattttgcgt ctccgagtta
     7741 atgatccacg tgtacaagat gtcacaatta ctgatgttca gatgttgggt gacctttcaa
     7801 tggctaaagt attttatact attcattcga cacttgcttc tgataatcaa aaagcacaaa
     7861 ttggtttaga aaaagcaact ggtactatta agcgtgaact tggcaaaaac cttactatgt
     7921 ataaaatacc agatttacaa tttgttaaag atgaatctat tgaatacgga aataagattg
     7981 atgaaatgtt gcgaaatcta gataaaaaag attaagaata ataaaaaata aaccatgtca
     8041 aaatcatggt ttatttttta tagctttaga gttgagatag ctagtattaa ggagttttat
     8101 tgatgatatc aatcatttga tactgtgctg ttttagcttc ttcaataggg taaattggat
     8161 aaacatgatt cattccctct tgtgcaatat agttaacggg gtgattagct gcttggagtt
     8221 gtgcagcata atcgcgaata tctgggaaga atatttctcg tgttccagta aataacgtaa
     8281 taggagcgag tttagtagca ggggcatttt taggactaac atatgtatgg ttagtattat
     8341 tagaaccatt tgcccaaatt tcacctacac gtgcgagacc ccaagcagat aggataggat
     8401 cggtatcttc gtatttggga atttctggat gtttcattgt cacatctagc catggagata
     8461 agaggataat atttttaggt tgaggaatag cctcttgccc tgattggtga gacaatgcat
     8521 gcgctaatcc tagtgctaat cctccgccag ctgaatcacc catcaaagtg agattagcgt
     8581 ttttttcatg aaagtgtcta taaaggttaa ccaatctagg gatagcataa tcataggtat
     8641 aacgaggagt ttttggataa attggtaaaa taatttttgc atcaagacta gttgagagcg
     8701 tcttaagcat gctgatatgg taaggcgttg gattatttaa gtaagagcca ccagctaaat
     8761 agaaaatggt cttttgatta gctttaccgt tgtcattcca agaatagact tgcatatcaa
     8821 aaagtctctc ttgaacaagc cctccattaa cagagtttgg gtctaagtaa taacccttat
     8881 cagattgttc acggctagca tttaagtact tttgaaatga gctttcatct tgaactttat
     8941 aataactttt atcgcgcttt agccaaaagt attgttcgat aaaccaactt ttccatgaac
     9001 gcccacttgc ttttgcatgt ggtgtacagg cgatggataa aatggtgata atactaaata
     9061 gtagcaatag ttttttgaag gacggtttca aggtattctc cttaatataa tgttattttt
     9121 gtgtaatatt attataataa aaccaacatc tttgtcaata gaataaagaa agatgagaaa
     9181 tgtttttcgg tttacaaatg tagatttatt tgctaaaatt ggtttacaaa cgtaaatcga
     9241 aagaggtgag aaaatgacta tttcaaacgc agaatgggaa attatgagag tggtttgggc
     9301 acagcagaat acaaccagta atgagatact agcagtttta ctagagaagt atgattggac
     9361 tccttcaact gtcaaaacct tattaagaag gctcttagat aaaggctacg tgagcagaga
     9421 aaaaatggga aaaggattta gctattcttc tttaattgat gaagatttag ctatgatgtc
     9481 agaggtagat agtgtattgc agaaagtttg ccagaccaaa catgttgcta ttgttagaca
     9541 cttattggaa agtataccaa tgacagagaa ggatcgatta aatttgcaag catcactaga
     9601 agctaagaaa ggcaaaacac ttgaacgtgt agcttgtaat tgcattcctg gtcagtgtca
     9661 atgccattag aaaggagaaa atatggctaa tagaaaagaa acatttttga ttgatgggat
     9721 gacttgtgca tcttgtgctt taacgataga aaaagctgtc aacaagttag accatgtgga
     9781 tagtgcagta gtaaatttag caacagagaa aatgacagtt acgtttgatg atacaacctt
     9841 atcgccaaac gtgattgaag agtgtgtttc tgagactgga tacgaagcat cgcttttcaa
     9901 agaggaaact tctaaaagcc aatctgaacg gcaccaatta gcaattgaaa agatgtggca
     9961 tcgcttttgg atgtcagcag ttgcaactat acctcttctc tatatttcca tggggccaat
    10021 gattaactta tggttaccat catttttgat gccagataag ggacctctaa actatggtgt
    10081 gatacaatta ttgttaacac taccggtgat gtattttggg cgtatctttt accaaaatgg
    10141 ttttaaagca ctttttaaaa ggcatcccaa tatggattca ttagttgcta tagccacaac
    10201 agcggctttc atttatagct tatatggatt gtacgaaata ttacagggtg acatacacta
    10261 cgctcaccag ctttatttcg aatctgttgc agttatttta acacttatta ctctaggaaa
    10321 atattttgaa atattatcca aagggcgtac ttcggcctca attgaaaaat tattaacttt
    10381 atcagcaaaa gaagcaagag ttatcaaaga tggtgaagac tatatggttc ctttggataa
    10441 agttaaaata ggtgaaacaa ttcttgtcaa acctggagaa aaaattcctc ttgatggcca
    10501 tgttgtagca ggagaatcaa gtattgatga atccatgtta acaggtgaaa gtattccagt
    10561 tgaaaaaaag gttggtagta aggtttacgg tgccagcatt aatgggcaag gtagcttaac
    10621 gattttcgtt gaaaaagaag ctggcggttc ccttctatca caaattatta acttagtaga
    10681 agttgcacag gcaagtaagg ctccaattgc taacttagca gacaaagtat caggagtctt
    10741 tgtccctttt gttattgtca ttgcaatttt atcaggtttg agttggtact taattctggg
    10801 gcaaagtttt gccttctctc tgaagattat gattgcagtc ttagtgattg cttgtccatg
    10861 tgctttaggg ttagcaactc ctacagctat tatggtggct agtggtaaag cagctgagaa
    10921 tggtatttta tttaaaggtg gtgaagtgtt ggaaaaggct caccacattg atacaatagt
    10981 ttttgataaa acaggaacct tgaccaaagg gaaaccagaa gtagttgcta tcaaaacata
    11041 tggggtagat aaagaggagt ttttaggtca agtagcatca gtagaaaaac tttcaaatca
    11101 tccacttagc caaacaattg ttaacaaagc aaaagagaaa gaacttccct taagggaagt
    11161 catggctttt aaaaatattt tgggttacgg tttgagtgca attattaatg gtaaaactat
    11221 gcttgtaggt aatgctaact taatgactaa gaacgatgtt aatttagatt tagcaaaggc
    11281 tgatatagaa atagctcaag aagaagcaca aactgtggtt tacgtttccg aaaatggagt
    11341 gttgagcggt ttaataactt taacggacca gctaaagaca gatagccaag aaacagttaa
    11401 gcagttacaa agattaggat ttaatttagt tttacttact ggtgataata aggctagtgc
    11461 agatgccata gcacaaaaat tgggtatcac gacagttgtt tcagaagttt tacctgatca
    11521 gaaagcaaac gttattttag aacttaaaga aaaaggcgga cagattgcga tggttggtga
    11581 tggcatcaat gatgctcctg cgctagctag cagtgatgta ggtatctcta tgagttctgg
    11641 aacagatatt gctattgaat cagcagatat cgtgttgatg aaacctgagt taacagatct
    11701 cttaaaagca atgactatca gtaaacaaac gattcaaatt attaaggaaa atttattttg
    11761 ggctttcttt tataacgttt tagcgattcc agtggcaatg ggtgttttac atctttttgg
    11821 tggtccctta ctcaatccta tgttggcagg cttagctatg gcgtttagtt cagtttcagt
    11881 agttttaaac gcacttcgct taaaagtctt aaaataataa agataataag actgaatttt
    11941 tatgaaggga gaagaatatg aaacatacct atcgagttag tggcatgaaa tgtgatggtt
    12001 gtgccaaaac agtttctgat aagttatcaa gtgtgatggg tgttgacgag gttaatgttg
    12061 atttgactaa aaatcaagtt gtcgtaagtg gaaaaacatt caaatgtctt ttaaaacgtt
    12121 ctttaaaaga tacaaagtat tcactagaag aggaaataaa ttaataacta aagaagtggt
    12181 tacagctttt aatttcttaa tttgagcagt ttctcacttc ttttttattc taaaaatgat
    12241 aaactagaat agaattagtt gtgaggagtg cagatgtcaa tcgatatttg gaacatttta
    12301 agtttggttg gaaccgtagc ttttgcaagt tcgggagcta ttgtggcgat tgaggaagaa
    12361 tttgatgttc tagggctttt tatcttaggt tttgtaacgg catttggtgg tggagctatt
    12421 agaaatgtac taattggctt gcctattgaa acactttgga gtcaaggaat tgctttttat
    12481 gcagcagcag cagctatttt gtttattatg attttcccta acttgttgag tggtaaggga
    12541 cgggatgccg aagttgtatc agatgctatt gggttagcag cgtttagtgt tcaaggagca
    12601 ctatatgcga ctcaatctca tcaacctctt agtgctgtca ttgtcgcagc agtactaact
    12661 ggtgcaggtg gtggtattgt cagagatgtt ctggctggcc gaaaacctgg ggtactaaga
    12721 agtgaaatat atgcaggttg gtcaatatta gtggggatca ttttatattt taaaattgct
    12781 aaaacaacaa ctgactatta tttgttagtg ttagttgtaa ctagcctaag gatgttgggg
    12841 tataagaaac aatggcactt gccagttgtc agaccacgta agaaatgata gaggagaaag
    12901 agatgataaa attaattgct attgatatgg atggaacgct tttaaatgat gaaaagaaaa
    12961 ttccaaagga aaatatccaa gctattaaag aagcaactca agcaggtatt aaaattgtac
    13021 tgtgtactgg gcgaccgatg tcaggtatac ttccttattt taatgaacta ggtttaacaa
    13081 aagaggagta tattatcatg aataatggtt gtagcactta tagcacgaaa gattggcaat
    13141 tgattgattc tgcaacttta acacatgatg aactcatctt tcttgaggag gttgttaaag
    13201 aatttcccaa cgtttgttta acgttaacag ctgaaaatac attttacgct gttggtgagg
    13261 aagttcctga aattgttgct tatgatgctg atttagtttt tacaaaagca aagtcaataa
    13321 gtttagatgc tcttcgcaat caagaagaaa ttgtctttca agcaatgtac atgggattag
    13381 atgcagatgt aactgctttt caagaagctg ttgaagaagc cttaatctcg aaatttagtg
    13441 gagttcgaag ccaagactat atttatgaga ttatgcctca aggagtaaca aaagcaagag
    13501 gcttaaaatc cttgatagca aaattaggac tggatattaa tcaagttatg gctattggtg
    13561 atgctcctaa cgatattgaa ttattagatt tagttcccaa tagcgttgct atggggaatg
    13621 catcagatga gattaaatct agatgtaaat atataacggt ggataataac caagccgggg
    13681 ttgctaaagc tatttatgac tatgctttac gttaaaatag atatagaata ataaaaggaa
    13741 ctttaaacac tgtttgttta gggttctttt gtttaataaa ctaacaaaat tcagaagttt
    13801 acagatagct tatgctataa tagacctatg acaaacaaga ataaattact tttaattgat
    13861 ggctcatctg tagcctttcg tgcttttttt gctctttata atcaaattga ccgctttaaa
    13921 aacaattctg gacttcatac taatgccata tatggtttcc atttgatgtt gaatcatatt
    13981 ttaggtcgtg tccagcctag tcatatttta gtggcttttg atgctggaaa aactactttc
    14041 cgtacggaaa tgtatgcaga ttataaaggt gggcgtgcta aaactccgga tgaatttcgt
    14101 gaacaatttc cttatattcg tcaacaatta gatgtcttag gcatcaaaca ttatgaattg
    14161 gaacattatg aagccgatga tatcattggt actcttgcaa aacaagccga agctagtaaa
    14221 gagcattttg atataacggt ggtttctggg gataaagatt taattcaatt aacagacact
    14281 aacacggtag tagaaatctc taaaaaggga gttgctgagt ttgaagaatt cacgccagct
    14341 taccttatgg aaaaaatggg aattacgccg agtcaattta ttgatttaaa agctttgatg
    14401 ggagataaat cagataatat tcctggggtt actaaaatcg gtgaaaaaac tggcttgaag
    14461 ttgctatcag aatatggttc cttagaaggg atctatgaaa atattgaggc tatgaaacag
    14521 tctaagatga aagaaaacct tatcaatgat aaggagcaag ctttcttatc aaagacccta
    14581 gctacaatca acatagcatc acctataaca atcggcttag aagatatcct ttatagtgga
    14641 ccgcaagata taaaagcctt gtctcaattt tatgatgaga tggattttaa acagtttaaa
    14701 gcagctctgg gtgaggagac aagtcaggaa gattttgagg ttgactttac tgaagtggag
    14761 caattaaaac cagaaatgtt ctccgataat gatttttatt actttgagat gttaggtgat
    14821 aactaccacg tagaagattt aattgggata gcttggggaa atagcgatac aatctacgct
    14881 actagtgatg tatcgttatt gcaagaagct ttatttaaaa aagctttatc aaaacctatc
    14941 aaaacttatg attttaaaag aagtaaggtc cttttaaatc gttttaatat tgatttacca
    15001 gagccagcct ttgatactag attggccaag taccttttgt ctactactga ggataatcta
    15061 gtatcaacaa ttgctaggtt atatacaaat ctgccactgg atactgatga tgctgtttat
    15121 gggaaaggtg ctaaacgcgc tataccagaa aaaacacgat ttcttgagca tctagctaaa
    15181 aaagtaaaag ttttagtgga ttctgaggct aatgttatgc aacagcttaa agctaatgag
    15241 caagaagaat tgctgtttga aatggagcaa cctttagcga atgttttagc taaaatggaa
    15301 attcgaggta ttaaggttaa gaaaaatacc ttgaatgaaa tggctattga aaatcaaaaa
    15361 gtcatcgaaa ccttaacaca agagatctat gaattggctg ggcaagagtt taatattaat
    15421 tctccaaaac agttaggaaa gttgttgttt gaaacgttag gtcttcctgt agaaatgact
    15481 aaaaaaacaa aaacaggtta ttcaactgca gtagatgtat tagagcgctt agctccgatt
    15541 tctcctcttg taactaaaat tttagagtac cgtcaaatca ctaaattgca atcaacctat
    15601 attattggtt tacaagatta tattttagaa gatggtaaaa ttcatacacg ttatgttcaa
    15661 gatttgactc agacaggtcg tctttcaagt tctgacccaa accttcaaaa tataccagtt
    15721 agactagagc aaggacggct tatccgaaag gcttttgttc catcagaaga caatgctgtt
    15781 ttattgagtt cagattattc tcaaatagaa ttgcgcgtgt tagcacatat ttcaaaagac
    15841 gaacatttga tcgctgcctt taaagaggga gcagatattc atacttcaac agctatgcgt
    15901 gtttttggta tcgaaaaacc tgagaatgtc actcccaatg atcgtcgaaa tgctaaggct
    15961 gttaactttg gaattgttta tggtatttca gattttggtc tatcgcataa tttaggaata
    16021 cctcgtaaac tagcgaagca atatattgac acctattttg agcgttatcc tggaataaaa
    16081 aactatatgg aaacagttgt tcgtgaagca aaagataagg gttacgttga aacacttttc
    16141 catcgtcgtc gttcactacc agatattaat tctcgtaatt ttaacattcg ccaatttgca
    16201 gagcgcacag caatcaattc accaatccaa ggaagtgcag ctgatattct taaaattgca
    16261 atgataaatt tagatagggt acttgataaa ggtggttata aatcaaaaat gctgttgcaa
    16321 gttcatgatg aaattgtact tgaagtccct aacgaggaga taggagcaat caaggagttg
    16381 gttacaaaaa caatggagtc agcaattagt ttatcggtac ctttgattgc agatgagaac
    16441 gcaggtgaga cttggtatga agcgaagtag tggacaacat acgtaaagga gtcattaata
    16501 tggtttatca ttttcaaaat ctaagtgatt ttatgttaaa aaattattta acaaaagcaa
    16561 agaccatcgc agttgtagga ttgtcagacc gtcaagaaac tgcagcttat caagtatcaa
    16621 aaataatgca agaagcaggt tatcaaatta ttccagttaa tcctaaaaat gcaggacaga
    16681 aaatacttgg gcaaatgaca tatgcctcac ttaaggatgt cactgaacat attgatattg
    16741 ttaatatttt tcgacgtagc gagtatttac ctgatattgc aagagaattt ttagaagtag
    16801 acgctgatat tttctgggct cagcttggac ttgaaagtca agaagcagag acaattctaa
    16861 agcaagcagg acacaaacag attgtcatga ataaatgtct gaaagttgaa taccaaaaat
    16921 tacactcaga aactaactaa ttaccgtgtt taataggcaa ataatataaa ttatgctata
    16981 ataaccatgc taaattaaag ttcgtgctgg aaaggagtcc tatggacagt cattcacatg
    17041 gtcatagacc tctcgatgct tatgaaaacg tcctagagca tctccgtgaa aaacgtattc
    17101 gaattactga gactcgaaaa gctattattt cctatatggt taatagtcgt gagcatccga
    17161 gtgcagaaaa gatttacaat gatttattac cagaatatcc taacatgagc ttggcaacag
    17221 tttacaataa ccttaaggtc cttgtggatg aggggtttgt gacagagcta aaactttgca
    17281 attatagtac aacttattat gattttatgg ggcatcagca cctaaacatt gcctgtgaag
    17341 attgtgggaa aatcgttgat tttgtcgatg ttgacttatt ggatatttct agagaagcac
    17401 accaacaaac aggttttgaa gtgactagag ttcagttagt agcttatggt atctgtccgg
    17461 agtgtcagag gaagcgtaag gataaatata aaaaagattg aaacgtaatt gttcaatctt
    17521 tttttgtttc tagactaaga gaaaaagttt aagttttact aagtaataat taagaaataa
    17581 tttataattg cttaaaaaat gatttatttt ttgttggtat actctcatta acaaaaagaa
    17641 agaggaatcc cttatgaaaa tatcacaata caataaatgg tcaattcgcc gattgaaagt
    17701 tggagctgca tctgtaatga ttgctagtgg aagcattgtt gctttagggc aatctcatat
    17761 tgtttcagca gatgagatgt cccaacctaa aacaaccatt acagccccaa cagctaacac
    17821 ttctacaaat gtagaaagta gtactgataa ggcattatca aaagttacta cgatggagac
    17881 aagttcagaa atgcctaaaa tgcaaaatat ggcaaaagtt gaaaaaacga gtgacaaacc
    17941 tatgatggtg gctacctctg ttagaaaaat gatggcaacg ccgacacctg tagcgatgac
    18001 caaaacgact agtgtagatg aggtaaagaa atcaactgat actgctttta aacagacagt
    18061 agacgttccg gctcattatg ttaatgcagc aaaagggaat ggcccatttc tagcaggtgt
    18121 aaatcaaacg attccatatg aagcgtttgg tggagacggc atgttaacgc gtttaatcct
    18181 taaagcttca gagggagcaa aatggtcaga caatggtgtt gataaaaata gtccattatt
    18241 accattgaaa ggcttaacta aaggtaaata tttctatcaa gtttctttaa atggtaatac
    18301 gacaggtaaa gaggggcaag ctctcttaga tcaaattaaa gctaatgata aacatagcta
    18361 ccaagccaca attagagttt atggtgctaa agatggtaaa gttgatttaa aaaacatgat
    18421 tagccaaaaa atggtaacca ttaacattcc tcacattaca actgatatgg aagtaaaaaa
    18481 ttctctaaag atggccttta aagaaaaagt agacgtccca gcaaaatatg ttagtgctgc
    18541 aaaagctaaa ggtccatttt tagcaggtgt taacgaaacg attccatatg aagcgtttgg
    18601 tggagatggc atgttaacgc gtttaatcct taaagcttca gagggagcaa aatggtcaga
    18661 caatggtgtt gataaaaata gtccattatt accgttaaaa gatttaacta agggtaaata
    18721 cttctaccaa gtctctctta atggcaatac agccggtaaa aaaggccaag ctctcttaga
    18781 tcaaattaag gcaaatggaa gccatactta tcaagcaact attactattt atggtacgaa
    18841 agatggcaaa gttgatatga atactatttt aggacaaaag acagtgatga ttcatattaa
    18901 tgtcgctaaa aaagatatga atagcacatc aatgatgatg aaaaaagata agatgacaat
    18961 gcctatgaag aaagaaatga cttcatctaa aataaatacc ggtatgatga tgtctaacaa
    19021 caagatgtca gctaatatgc aaatgtcatc acaagccaaa tcaaatgata aagctggtaa
    19081 aaaaatgtct atgatgtcta aaaatttacc taatacaggt gaaacaaagc aacaaaatgt
    19141 aggtgtgcta ggtatgctta gtctagcctt tgcgacaggt ctaactgcgc taggccttaa
    19201 aaaatctaaa caaagatagg gatagtattt aattcagaac accaaatttg tttaaaatta
    19261 ttgagggtta tcgtggtaaa atagtgttat actagaggta tagcactatt ttgactcatt
    19321 aggagagtag aatgacacaa aaattattat tagttgacga tgaatttgaa attattgata
    19381 ttaatagacg ttatctagag caagcaggat atgaagtgtc agtagctgcg gatggcatag
    19441 aagcactcaa agaggtagat gagaatcgtt ttgacctaat tattagtgat attatgatgc
    19501 ctaaaatgga tggctatgat tttattagtg aagttctagt tcgagaaccc aaccaacctt
    19561 ttctctttat aaccgctaaa gtatctgaac cagataaaat ttactctttg agtatgggtg
    19621 cagatgactt tatttccaag cctttcagtc ctagggaatt agttttacgt gttaaaaata
    19681 ttctaagacg tatctatggg aatcatcaac aatctgaagt attgacgatt ggtgatttgg
    19741 ttatcgacca aaaacaacgt ttggtaatgg tagactgcaa tacaatttcc ttaaccaata
    19801 aatcatttga cttgctttgg atattagcaa atcatttgaa tcgtgttttt tcaaaaacag
    19861 agctttatga acgtgtctgg ggcgaagaat ttttagatga cactaatact ttgaatgttc
    19921 atatccatgc cttacgcaat gaccttgcta aatttagtac agacaatacc ccaactatca
    19981 aaacagtttg gggattaggg tataaattag aggaataaaa tgaaattaaa atattatatt
    20041 gttattggtt accttatttc tatgttaata actgtagcag gtgttttctt tgggcttaat
    20101 catatgctga ttgagactcg aggtgtttac tacatccttt cagttacaat tattgcctgt
    20161 atcgtaggtg gtatagttaa tttgttctta ttatcaagtg tatttacatc tttgaaaaag
    20221 ttaaaacaaa aaatgaagga tatttctcaa agacgttttg atacaaaagc acaaatttgt
    20281 tcaccacaag aattcaagga tttagagaca gcctttaacc aaatgtcttc agagttagag
    20341 tcaacgttta aatctctaaa cgagagtgaa cgcgaaaaaa caatgatgat tgcacaactt
    20401 tcacatgata tcaagactcc aattacctct atacaatcaa cagtagaagg tattttagat
    20461 ggtattatat ctgaagaaga agttaactat tatctcaata ccatttctcg tcaaacaaac
    20521 cgtcttaacc atctagttga agaattaagt tttattacat tagagactat gtcagacacg
    20581 gctgaacctc ataaagaaga aacaatttat ctggataaac tactgattga tattttatct
    20641 gaatttcaat tggtgtttga aaaagaaaat cggcaagtaa tgattgatgt tgcgccagat
    20701 gtttcgaaat tatctagtca atatgataaa ttatcacgaa ttttattgaa tttgattagc
    20761 aatgcagtca aatattctga tcctggaagt cccttgacta tcaaggccta ttcaaatcgt
    20821 caagatatcg ttatcgatat tattgaccaa ggctatggga taaaagatga agatcttgct
    20881 tctattttta atcgttttta tcgtgtagaa tcttcacgaa atatgaagac aggaggacat
    20941 ggcctaggtc tttatattgc gcgacaactt gcacatcaac tgaatggtga tattttggtc
    21001 gaaagtcagt atcaaaaagg cagtaagttc tctttagtac ttaaattaca aaaatagcta
    21061 ggaattattc ctagctattt tttgtaatgt ttctataaaa gattaagcgc gcagtagttt
    21121 ggtaaggaag gagatagata tagactaatc cgaaactgaa gattgtaagg aaatgccaac
    21181 caataaaact taagtctaaa aggaagagtc taagtttatt accattcatt agttctcgac
    21241 tagcaactag tactttagat ggtccctcat agtttccact ttctaattga gtgtaaaata
    21301 ctaaagttga gatactatag gagtaatatt gttgaacgag ttttataact gccttagtag
    21361 taattaggct actaacaact attaaaacag gggcaaggat aaggttacca ctcaaaagta
    21421 gcctaaaact tgaaatgatg attagtagac caaaagtact tagtagataa aagaaagata
    21481 aaattagtcc ttttagaaca agtactgaga gtaatttcca gaaaatatgg cttgaaaata
    21541 cataagtatt gtccgagaat ctaacgttta gtttctgatt tttgacgaca tctaacatta
    21601 caaaactgac tgaaataata aaaagtgtgt aaaagaaata aactggaaga ggtacaa
//
LOCUS       DATRSG010000031        21604 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRSG010000031 DATRSG010000000
VERSION     DATRSG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21604
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.031"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..963
                     /gene="gtfB"
                     /locus_tag="VBD89_001145"
     CDS             <1..963
                     /gene="gtfB"
                     /locus_tag="VBD89_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO7135683.1"
                     /translation="FGWCFARTHFDKNQNVTTKSYFDKDGKEVIVENFRTGVIILNWL
                     NKDYFFDNRVAFLNFYFSLMGWNLSRIWYNSLSTPFFVSYRMTYPGEDILFWQEDIED
                     TIPANMRVLLESTNTRTQKVIVQKKNTYHKIKSMLPKEQQEKIGYLGFIYPNKKNNKG
                     RKDIFILTNSDQIEHLEVLVHHLSDYHFHIAAYTEMSFKLMSFSQEQNVTLYPNISRT
                     DLDNLFEICDIYFDINHGNEVDDVIRRAFEYNHLIFAFDNTCHNRELVLDSNIISHTT
                     CEQLINLMKNLSGSIMYLLEQQREQTSNETKERYKEILGGYGNA"
     gene            956..1123
                     /locus_tag="VBD89_001146"
     CDS             956..1123
                     /locus_tag="VBD89_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135684.1"
                     /translation="MPKRNELLNKEIKMSIDKLRYKEPESEHDKRPTFYLVVLILVTV
                     AVILSLFKYFL"
     gene            1177..2172
                     /locus_tag="VBD89_001147"
     CDS             1177..2172
                     /locus_tag="VBD89_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7135685.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDV
                     VPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNI
                     TLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLI
                     MKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGF
                     IIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM"
     gene            2180..3892
                     /locus_tag="VBD89_001148"
     CDS             2180..3892
                     /locus_tag="VBD89_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO7135686.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLIDSQNYIDEIVKTITIDDT
                     EFRDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGSTDGSGNICEKYAM
                     KDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDESYLNYLYNKLRANKSD
                     ISATTYTFFNEEEKCFVYFATDDNYFEKTFFAQEWLEQEHSYHDNLKQIFPLLSMKLF
                     RKSLFNNITFPLEDLTGEYTLYNVYLLSRHITFSHIGLYITSQVFSHTEIKRFTGHDI
                     EKMLSRKEERLALLTMVGFNSEKYIKNYKETLLNYQSESLNIGDINLYNKIKQKIALI
                     DYCY"
     gene            4016..5365
                     /gene="gorA"
                     /locus_tag="VBD89_001149"
     CDS             4016..5365
                     /gene="gorA"
                     /locus_tag="VBD89_001149"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO7135687.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(5408..5860)
                     /locus_tag="VBD89_001150"
     CDS             complement(5408..5860)
                     /locus_tag="VBD89_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135688.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            6112..7257
                     /locus_tag="VBD89_001151"
     CDS             6112..7257
                     /locus_tag="VBD89_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7135689.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            7259..8473
                     /gene="thiI"
                     /locus_tag="VBD89_001152"
     CDS             7259..8473
                     /gene="thiI"
                     /locus_tag="VBD89_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO7135690.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            8575..9753
                     /locus_tag="VBD89_001153"
     CDS             8575..9753
                     /locus_tag="VBD89_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO7135691.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    9829..9908
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            9943..10257
                     /gene="rplU"
                     /locus_tag="VBD89_001154"
     CDS             9943..10257
                     /gene="rplU"
                     /locus_tag="VBD89_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO7135692.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            10264..10602
                     /locus_tag="VBD89_001155"
     CDS             10264..10602
                     /locus_tag="VBD89_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO7135693.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            10624..10917
                     /gene="rpmA"
                     /locus_tag="VBD89_001156"
     CDS             10624..10917
                     /gene="rpmA"
                     /locus_tag="VBD89_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO7135694.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            11133..12038
                     /locus_tag="VBD89_001157"
     CDS             11133..12038
                     /locus_tag="VBD89_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7135695.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            12047..12511
                     /locus_tag="VBD89_001158"
     CDS             12047..12511
                     /locus_tag="VBD89_001158"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO7135696.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWLFTLITIFVVCVAIIYLMKHINGSYWLLISLTLIISGGLGN
                     FIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
     gene            12495..13385
                     /locus_tag="VBD89_001159"
     CDS             12495..13385
                     /locus_tag="VBD89_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7135697.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            13561..14082
                     /gene="pyrR"
                     /locus_tag="VBD89_001160"
     CDS             13561..14082
                     /gene="pyrR"
                     /locus_tag="VBD89_001160"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO7135698.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            14131..15201
                     /locus_tag="VBD89_001161"
     CDS             14131..15201
                     /locus_tag="VBD89_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO7135699.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            15257..16855
                     /locus_tag="VBD89_001162"
     CDS             15257..16855
                     /locus_tag="VBD89_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO7135700.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            17057..18301
                     /locus_tag="VBD89_001163"
     CDS             17057..18301
                     /locus_tag="VBD89_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO7135701.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSFPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            18304..19014
                     /locus_tag="VBD89_001164"
     CDS             18304..19014
                     /locus_tag="VBD89_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135702.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            19029..20276
                     /locus_tag="VBD89_001165"
     CDS             19029..20276
                     /locus_tag="VBD89_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135703.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            20405..20677
                     /gene="rpsP"
                     /locus_tag="VBD89_001166"
     CDS             20405..20677
                     /gene="rpsP"
                     /locus_tag="VBD89_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO7135704.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            20687..20929
                     /locus_tag="VBD89_001167"
     CDS             20687..20929
                     /locus_tag="VBD89_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO7135705.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            21061..>21604
                     /locus_tag="VBD89_001168"
     CDS             21061..>21604
                     /locus_tag="VBD89_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7135706.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSN"
BASE COUNT         7315 a         3153 c         4255 g         6881 t
ORIGIN      
        1 ttcggatggt gttttgcacg tacacatttt gataagaatc aaaatgtgac tacaaaatct
       61 tatttcgata aggatggtaa agaagttatt gttgaaaatt ttagaacagg agttataatc
      121 cttaattggc taaataaaga ttattttttt gataatcgag ttgcattctt aaatttttat
      181 tttagcctta tgggttggaa cttgtctcga atatggtaca actccttatc aactccattt
      241 ttcgtttctt accggatgac ttatccagga gaagatattt tattttggca agaagatata
      301 gaggatacta ttcctgctaa tatgcgcgtg ttattagaaa gtacgaatac tagaactcag
      361 aaagtaattg ttcaaaagaa aaatacatat cataaaataa agtcaatgtt acctaaagaa
      421 caacaagaaa aaattggtta tttaggcttt atatatccta ataaaaagaa caataagggt
      481 aggaaagata tttttatttt gacaaactct gaccaaattg aacatttaga agtgttggtt
      541 catcacttgt ctgattatca ttttcatatt gcagcgtaca cagaaatgtc ttttaagttg
      601 atgtcattta gccaagaaca aaatgtaacg ctatatccga atatttcaag aactgattta
      661 gacaacttat ttgaaatctg tgatatttat tttgacatta atcatggaaa tgaagttgat
      721 gatgttatta gaagggcatt tgaatataat caccttatct ttgcttttga taatacctgt
      781 cataacagag agttagtatt agatagcaat atcatttctc acacaacctg tgaacaattg
      841 ataaatttaa tgaaaaattt atcaggctcc attatgtatt tgctagagca acaaagagaa
      901 caaacaagta atgaaacaaa agagcgttat aaagaaatat taggagggta tggaaatgcc
      961 taaaagaaat gaattactca ataaagaaat taaaatgagt attgataaac ttagatataa
     1021 agaaccagag agtgaacatg acaagcgacc tactttttat ttggtagtac ttatacttgt
     1081 tactgtagca gttatattgt cgttatttaa atatttttta tagattccta ttaatggtag
     1141 aaaagaagcc tgttgttttt tagatagggg aagtgtgtga ggacatatat tacaaacttg
     1201 aatggacatt caatcactag tacagcacaa atagctcaaa acatggtaac agatatagca
     1261 gtaagcttag gttttcgtga gctgggaata cattcttatc cgattgatac tgattctcct
     1321 gaggaaatga gtaagcgttt agatggaatc tgttccggac ttagaaaaaa tgatattgtc
     1381 atatttcaga cacctacatg gaacactaca acttttgatg aaaaattatt tcacaaatta
     1441 aaaatatttg gtgtaaagat tgttattttt atacatgatg ttgtaccgct aatgtttgat
     1501 ggaaattttt atttgatgga tagaactata gcttattata atgaagcaga tgttttaata
     1561 gcccctagtc aagcaatggt cgataagctt caaagttatg gcttgacagt aaaaaaaatc
     1621 ttagttcaag gaatgtggga tcatcccacc aatattactt tacaagcagt taatcataag
     1681 aagcttgttc attttcctgg gaacccagag cggtttaact tcataaagaa ttggagaatt
     1741 cctaccgagt tacatgttta tactgaccat aacatgcaat tacccaccac tgtggttaaa
     1801 gagccatatc aatcggacga acaattaatt atgaaaatga gcgaaggggg atatggctta
     1861 gtctggatgg atgaccgtga taaacaatat caatctcttt attgccctta caaactgggc
     1921 gcttatatag cagcaggaat tccagtgatt attcaaaaag gtattgctaa tcaagatata
     1981 atagaaaaaa ataatttggg atttattatt gaaaaaattg atgatattag taatatagtt
     2041 gaaagtacta ctgaagagga atatatggaa attgttagcg atgtcaggcg gtttaatcct
     2101 ttagttagac aaggatattt tacgcgaaaa ctattaaccg atgcagtatt tagtgcatta
     2161 aatagcatgt gaggggaata tgaaagaact agaagaaatc gtagttttcc ctataaacaa
     2221 aagtttggat tacattttgg aaaatcattc gtcagttatt cgctttgggg acggtgaatt
     2281 tgacattata atgggaaaat cgattcctta tcaagattac aacccagagt tagcagatag
     2341 gttattgcaa cttcttcata agcaaagtac gaatgatcta ttagtatgtc ttccagatat
     2401 gtttcaaaat cgacaaagat ataatcaatc ggcacaactt ttttggaaaa atcattttca
     2461 aaaatttggc aatttctata ggcaacattg ttatcaagat tggtatggtt cttcttttat
     2521 ttcaaggcca tatatggact tacaagataa ggagtctgct gtagaatcat ttaaaaaatt
     2581 aaagttactt tgggacaaac gcgatatttt ggtagttgaa ggggaaactt cgcggtcggg
     2641 tgtaggtaat gatcttttta ataattgcaa tagtttaaaa aggattattt gtccttctaa
     2701 aaatgcttat ataaaatatg atgaaattct tgaggctgtt gaaagatatg ctcaagggag
     2761 gataatctta ttgatgctag gtccgactgc aaaaatttta gtaagagatt tatctgatag
     2821 aggatttcaa gccattgata tcggtcatat tgtttctgaa tatgaatggt ataagatggg
     2881 agctactcaa aaaataaaat tttctcataa acacaccgct gagtttaacg atgatgaaaa
     2941 tattaattta attgacagtc agaattatat tgatgagatt gttaaaacaa taactatcga
     3001 tgatactgaa ttcagggata ctgaattaat atcaatcata cttcctgtct ataatatgtc
     3061 tggttcttta tcaaggtgtt tagattctat ccttaggcaa gtttactcaa cttttgaggt
     3121 gatcttagtc aaccatggtt ctacagatgg aagtggtaat atttgcgaga aatatgcgat
     3181 gaaggatgag cgtattcgtt tatttcatga agaacatcaa gttattacat ctgtatggaa
     3241 cttcgcatta gagaaatcta aagggaagta tgttactttt gtgaatccag atgattttct
     3301 tgatgagtct tacttaaatt atctttacaa taaattaaga gctaataaat ctgatatctc
     3361 agcaaccacc tatacttttt ttaatgaaga ggaaaaatgc tttgtttatt ttgcaacaga
     3421 tgataattat tttgagaaaa cattctttgc tcaagaatgg ttagagcaag aacatagtta
     3481 ccacgataat ttaaaacaaa tatttcctct cctttctatg aagctcttta gaaaatcact
     3541 ttttaacaat atcacttttc ctttagaaga tttaacagga gaatacacac tgtataatgt
     3601 ttatttgcta tcaaggcaca ttacattttc tcatattggt ttatacataa cctctcaagt
     3661 tttctctcat actgaaataa aaagattcac gggacatgat atagagaaga tgctatctag
     3721 aaaggaagag aggttagccc tgttaacaat ggtagggttt aattctgaga aatacatcaa
     3781 aaattataag gaaactttat taaattatca aagtgagtcg ttgaatatag gtgatattaa
     3841 tttatataat aaaattaagc aaaagattgc attgatagat tattgttact agaaataatt
     3901 ttaacagcaa gttaggatat gataatcggt aatataaatt gtttacgtag cagaaaattg
     3961 tgactgtaaa cactttgtga tagagtatag gtatcaatga ttcaaggaga gagtaatgtc
     4021 aaaacaatat gattatatcg ttattggtgg aggtagtgca ggcagtggta ccgctaatag
     4081 ggcagccatg tatggagcaa aagtcctgtt aattgaaggt ggacaagtag gtggaacttg
     4141 tgttaactta ggttgtgtac ctaagaaaat catgtggtat ggtgcacaag tttctgagac
     4201 actccataag tatagttcag gttatggttt tgaagccaat aatcttagtt ttgattttac
     4261 tactctaaaa gctaatcgcg atgcttacgt gcagcggtct agacagtcgt atgccgctaa
     4321 ttttgagcgt aatggggtcg aaaagattga tggatttgct cgttttattg ataaccatac
     4381 tattgaagtg aatggtcagc aatataaagc tcctcacatt actattgcaa caggtggaca
     4441 ccctctttac cctgatatta ttggaagtga acttggtgag acttctgatg atttttttgg
     4501 atgggagacc ttaccaaatt ctatattgat tgttggggcg ggctatatcg cggcagaact
     4561 tgctggagtg gttaatgaat taggcgttga aacccatctt gcatttagaa aagaccatat
     4621 tctacgcgga tttgatgaca tggtaacaag tgaggttatg gctgaaatgg agaaatcagg
     4681 tatctcttta catgctaacc atgtacctaa atctcttaaa cgcgatgaag gtggcaagtt
     4741 gatttttgaa gctgaaaatg ggaaaacgct tgtcgttgat cgtgtaatat gggctatcgg
     4801 ccgtggacca aatgtagaca tgggacttga aaataccgat attgttttaa atgataaaga
     4861 ttatatcaaa acagatgaat ttgagaatac ttctgtagat ggcgtgtatg ctattggaga
     4921 tgttaatggg aaaattgcct tgacaccggt agcaattgca gcaggtcgtc gcttatcaga
     4981 aagacttttt aatcataaag ataacgaaaa attagattac cataatgtac cttcagttat
     5041 ttttactcac cctgtaattg ggacggtagg actttcagaa gcagcagcta tcgagcaatt
     5101 tggaaaagat aatatcaaag tctatacatc aacttttacc tctatgtata cggctgttac
     5161 cagtaatcgc caagcagtta agatgaagct cataacccta ggaaaagagg aaaaagttat
     5221 tgggcttcat ggtgttggtt atggtattga tgaaatgatt caaggttttt cagttgctat
     5281 caaaatgggg gctactaaag cagactttga tgatactgtt gctattcacc caactggatc
     5341 tgaggaattt gttacaatgc gctaacgcta tcaaagaata caaaaaaagc tagggaacct
     5401 agctttttta aggtgttgtt gtattttcaa tagttgaggt atcggtagta gtattatttg
     5461 tttgatcatt gttagtacta gatgatgttt gatcgttatt ggcagcagaa tttgaactgc
     5521 tactcggagt actactagca gaggaatttt ctagtgtttg gctggatgat gcccttgtct
     5581 catctttttc agtagaaact tggttactag aattttgtcc actttctctt ataatgacgg
     5641 tttttgaagt agaagaatca gtcttactag agtctttgtg attattaatc gcattagtaa
     5701 tagtaataga ggcaacgata atacttaaaa cactaccgac taatgcaatt gttttttgaa
     5761 gtgcagaaaa acctgttttt atatgcttag cgggtgattg agttgaatca gtattagtct
     5821 tagtagactt tttctgttgt cttgaatatc cttttgacat gacgccctcc ttaaatgtac
     5881 atcaactatt atatgatttt atcctaaaaa aagctaaaga aaaaataatt taaaacattt
     5941 aaataagcaa cctaggttat taagctaagc tatgttagtg taataggtag tgagttttgt
     6001 gataaattat gagaattatc ataaatattg ttttgggatg ttcacaaact aatataaata
     6061 gtaggaaaat tatgctataa taacactgat tgactttaga aggacagatt tatgatttat
     6121 tttgataatt cggcaactac tattccctat ccagaagttt taaggactta tcaagaagta
     6181 gctagtaaaa tctatggaaa cccctcaagt ttacatgagc taggaacaac atcttctcgg
     6241 attttagagg ctagccgtaa acaaattgct agtttacttg agcttaaagc aaatgaaatt
     6301 ttctttacct ctggtggtac agaggccgat aattgggtta ttaagggtct tgcgtttgag
     6361 aaacaacatt ttggaaatca tattattgtt tctgatatcg aacacccagc tgttaaagag
     6421 tctgctaaat ggcttggaga gtatgggttt gagattgatt atgcgccagt agatgataaa
     6481 ggatttgtag acgtagaagc actggtcaaa ttaattaaac cagaaacgat tcttatttca
     6541 attatggcaa taaacaatga aattggttct attcagccaa taaaagctat ttcagacttg
     6601 ttgtcagata aacctactat atcgtttcat gttgatgctg ttcaagcaat aggtaagata
     6661 ccgactaaag attatttgac tgagcgagtt gattttgcat ctttttctag tcataaattt
     6721 cacggcgttc gtggggttgg ctttctatat attaaagaag gaaaacgtat ttcaccatta
     6781 ctaacaggtg gtggacaaga atctgattta cgttcaacta ctgagaatgt cgcagggatt
     6841 gcagctactg ctaaagcttt acgaatggtt atggacaagg aagtagtagc tattcctaaa
     6901 ataagtaaaa tgaaaaccat tattcatgat gaattagcca aatatgagga tattacgtta
     6961 ttttcgggta aggaaggttt ttcacctaac attatcacgt ttggtatcaa gggtgtccga
     7021 ggagaagttc ttgtccatgc ctttgaggga catgatattt ttatctcgac aactagcgct
     7081 tgctcttcta aagcaggtaa accagcagga actcttatag caatgggaat ttcaactaag
     7141 ttagcccaaa ctgctgtacg tatcagctta gatgatgata atgatatggg acaagtagag
     7201 caatttttaa ctatttttaa acaagtttat gaaaaaacta agaaagttag aggataagat
     7261 gcagtattca gaaattatga ttcgttatgg agaactctct actaagaaga aaaaccgtat
     7321 gcgcttcatc aataagttaa aaaataatat ggagcatgta ctctccattt atccagatgt
     7381 ttcagtaaaa acagatcgtg atagaggaca tgtatatctc aatggtacag attatcatga
     7441 agttgcagag tccttaaaag agatttttgg tatccaagct ttttctccat cttttaaagt
     7501 agaaaaaaat gttgatacat tggtaaaagc tgtccaggaa attatgactt ccgtttataa
     7561 agatgggatg acttttaaaa ttaccgcaaa acgtagtgac cactcatttg aattggatag
     7621 ccgtgctcta aatcatactt taggagatgc cgttttttca gtcttgccaa atattaaggc
     7681 tcagatgaag caaccagata tcaatcttaa agtcgagata cgagatgagg ctgcttatat
     7741 ttcatatgag gatattaggg gtgcaggagg attaccggta ggaacatctg ggaaagggat
     7801 gctgatgttg tctggtggga ttgattctcc ggtagcaggt tacctagcgt taaaacgtgg
     7861 tgtagatata gaagcagtcc attttgcaag tcctccttat actagcccag gtgcattgaa
     7921 aaaagcacat gatttaacac gtaaattgac aaaatttggt ggtaatattc aatttattga
     7981 agttccattc acagaaattc aagaggaaat taaggaaaaa gctcctgaag cctacttgat
     8041 gacgttaaca cgtaggttta tgatgcgtat tacagatcgt attcgtgagg accgaaatgg
     8101 tcttgttatt attaacggtg aaagtttagg gcaggtggca agccaaacgc tagaaagtat
     8161 gcaagctatt aatgctgtca ctgcaacacc gattattcgt cctgtggtca cgatggataa
     8221 gctagaaatt attgatattg ctcaaaaaat agatactttt gatatttcaa ttcaaccatt
     8281 tgaggattgc tgtacaattt ttgcaccaga tcgcccaaaa actaacccta aaattaagaa
     8341 tacagaacag tatgagaaac gtatggatgt agaaggtctg gtagagaggg cagttgcagg
     8401 gattatggta actactattc aacctcaagc agatagtgat gatgttgatg acttgattga
     8461 cgatttatta taatgaattt ttaaactaca gatttactct gtagtttttt attaaaatga
     8521 ttgtgctgtc ataaggttat aaaaaatgct ataatacgaa taaaggaggc aagtatggca
     8581 agaatccctt atcaaaaaac acttattttt tgtgtagccg ttattattgc tatttttatt
     8641 ttggggttat ctaaggattt agcacaatct aaagggcaaa aggttgcaaa taataatact
     8701 gtaaaaacag cacgtgtggt tgcaaacgga gatattttgc tgcacgatgt actatatgcc
     8761 agtgcgcgcc aacccgacgg cacttataat tttactcctt attttaaaga ggtaaaatct
     8821 tggattgaga gcgctgattt agcgattggt gattatgaag ggacgattag ttcagaatat
     8881 cctttagcgg gctatccttt atttaatgct cctaatgaga ttgcaactac tatgaaagaa
     8941 actggatatg atgtcgttga cttagcacat aatcatattc tggattcaca actggcaggt
     9001 gctatcaata ctgtcaagac ttttaatcgt ctgggattgg atactattgg ggtttattta
     9061 aaggatagaa ataaagaaga tatcttaatt aagcgtgtaa atggtattaa aattgctatt
     9121 ttaggttatt cgtatggcta caatggtatg gaagctaatg tttcaaagag tgactatgaa
     9181 aaacatatgt ctgatttaga tactaaaaag attaagcaag atattaaaaa ggctgaaaaa
     9241 gaagcagata tcaccattgt catgccacag atggggatag aatatcaaaa aaaacctact
     9301 acagaacaag taatacttta tcatagtatg attaaatggg gggcagatat tatattcgga
     9361 ggacaccctc acgttgttga accgtctgaa gtgataaaaa aagatggcca gaaaaaattt
     9421 attatttact ctatgggaaa ttttatttca aatcagcgtt tagaaacggt tgatgatatc
     9481 tggacagaaa gaggattatt aatggatgta accatagaga aaaaaggtca aaaaacagta
     9541 attaaaaaag ttaaggcgca tccaacacta gtagaagcaa aagcaaatgg ccgttatgtt
     9601 gaagaaggct ttccagctta tgattatagg acgctagttt tagaggatta tattgaaggt
     9661 ggtaagaaac gttctacaat tgatcagact atgaaagata aagttgataa agcatataag
     9721 gaaatgaatg agttagttaa tttgaagtgg taaagttgat aattttattt tgttttatct
     9781 tgcataaaaa aagagtttat gataaaattt gaatgttgac taaatgcaca tcctgtgcaa
     9841 ccgcgcgaaa atcgttagat taagtagggc aaggctctca cgatttatag gcgagtcttc
     9901 acagagggaa acctcacaag aaatttttat aggaggtgca tcatgagcac atacgcaatc
     9961 atcaaaactg gtggtaaaca agttaaagtt gaagtaggac aagctatcta cgttgaaaaa
    10021 cttgacgttg aagcaggagc agaagttaca tttaacgaag ttgttcttgt tggtggtgaa
    10081 acaactaaag ttggtactcc agttgttgaa ggagctactg ttgttggtac tgttgaaaaa
    10141 caaggcaaac aaaagaaagt tgtttcttac aaatacaaac ctaaaaaagg tagccaccgt
    10201 aaacaaggtc accgtcaacc ttatacaaaa gttgttatca acgcaatcaa cgcttaattt
    10261 ttcatgatta aagcaacttt tactcgtaat caatcaggct atctttatag tgctgaaatc
    10321 agcggtcatg ctggtagtgg cgagtacggc tttgatgtga tttgtgcagc tgttagtact
    10381 ttatcaatta attttattaa ttcattagag gctttaacaa cctgccaagc gcaattgatt
    10441 atcaatgacg ttgaaggtgg ttatatgaag atagatttat cttctatacc acaacataaa
    10501 gaggataagg ttcaattatt atttgagtct taccttcttg gaatgactaa tctgtctaaa
    10561 gattcttctg aatttgttag tacagtagtc atgacacact aatttaagag aggacacaac
    10621 attatgttaa aaatgaatct tgctaacttg caacttttcg cccacaaaaa aggtggaggt
    10681 tctacatcaa acggacgtga ttcacaagca aaacgtcttg gagctaaagc agctgacgga
    10741 caaacagtat caggtggatc aattctttac cgtcaacgcg gtacacacat ctatccaggt
    10801 gctaacgttg gacgtggtgg ggacgataca ctttttgcta aagttgaagg tgttgttcgt
    10861 ttcgaacgta aaggacgcga taaaaaacaa gtgtctgttt acccaatcgc aaaataatgc
    10921 ctagttaaga ctttatgact attcataagg tcttttatta ttgtcagagc tttcattttg
    10981 taacaaaaga caatatgtta agagatttat cattaatact cataaacttt gttgttcttt
    11041 tatagtgaaa tagtcctaat tcatgaagaa ttttccgcaa ggatagttat tttttggtat
    11101 aatgaagggt actaaaatga ggaagtgata aaatgaatat tcaacaactg cgttatgttg
    11161 ttgctatcgc aaatagtggt acttttcgtg aagcagctgc aaaactattt gttagtcagc
    11221 caagtttatc tgttgccgtt cgtgatttgg aaacagaatt agggtttcag atttttacgc
    11281 gtacaactac tggtgcagtg ttgaccaatc aaggcatgac tttttatgag aatgctttag
    11341 aagttgtgaa aagctttgat tcattcgaaa aacagttttc gcaatctgaa gctacagagc
    11401 aagaattctc aatagcaagt caacattatg attttctacc tcctttaatc acagcgtttt
    11461 caaaatgtaa tgataatttt agctatttcc gtattttcga atcaacaaca atacgtatct
    11521 tagatgaagt tgctcaaggg aatagcgaaa taggtatcat ttatatcaat agtcaaaata
    11581 aaaaaggtct acttcagcgg ttggacaaat tggggttaga gtttgtagag cttattcctt
    11641 ttaaaactca tatttatctt gggaaagacc atcccttggc tagtaaaaca tctctgataa
    11701 tgacagattt agaaggatta ccaaccgtac gttttacaca ggatagagat gattatcggt
    11761 attactcgga gaattttgtg gaagtacttg atagttctgt tacctataat gttactgata
    11821 gagcaacatt aaatggtatt ttagaaagaa cccaagctta tgcgacgggc tcaggattct
    11881 tggatagccg cagtgtgaat ggtatcactg ttattccatt agaagatcat ctagataacc
    11941 aaatgattta tatcaaacga aaagatcgta atttatctca gatggcttta aaatttgttg
    12001 ctgttatgga agagtatttt aagggattta attcatagga gaagtaatga gaaagattat
    12061 tataccaata ataactattt tgttgattgc tttagatcaa ctatctaagt tatggattgt
    12121 taaacatatt gaactaaacc aaattaagga attcattcca aatatcgtta gtctaactta
    12181 tctgagaaat tatggtgctg cattttctat tttgcaaaat caacaatggc tttttacctt
    12241 aattacaatc tttgttgttt gtgttgctat tatttatttg atgaaacata ttaatggaag
    12301 ttattggtta ttaatttctc taaccttaat catctctggt ggattaggaa actttataga
    12361 tcgcttaaga ttaggttatg tcgttgatat ggtgcattta gattttatta attttgctat
    12421 ctttaatgta gcagactcat atctgacaat cggtattatt tgcttaatga ttgcactttg
    12481 gaaagaggaa agtaatggaa atcacaatta aaatagcagg agttaggcta gataaagctt
    12541 tagctgataa ttctgaactg tcgcgtagtc aagctaatga agaaattaaa aaagggattg
    12601 tgttagtaaa tggacaagta aaaaaagcga aatatactgt tcaagaagga gatcggatta
    12661 cttttgatat tcctaaagag gaagtactag attatcaagc tgaaaatatc cctttagata
    12721 ttatctatca agatgatgac gttgctgtgg ttaataagcc gcaagggatg gttgttcacc
    12781 cttctgcagg ccacagttcg ggcaccttag ttaacgcctt gatgtatcat attaaggatt
    12841 tatcatcaat taatggcgtt gttcgcccag ggatcgttca tcgtattgat aaagatactt
    12901 ctggtttatt gatggttgca aaaaatgata gagctcacca agtgttggct gaagagttga
    12961 aagataaaaa atcgcttcgt aaatatttag caattgtgca tggtaattta ccaaatgatc
    13021 gaggcgtgat tgaggctccg attggtagaa gtgataaaga tcgtaagaag caagccgtga
    13081 ctgctaaagg taagccagca ataactcgtt ttcatgtttt agaacgtttt ggtgattata
    13141 ccttagttga attgtcattg gagacaggtc gaacgcacca aattcgggtt catatggctt
    13201 atattggcca tcctctagcc ggtgatcctg tctacggtcc ccgcaagact ttagggggta
    13261 aaggacagtt tttgcatgca caaacactag gttttacgca ccctagtaac ggagagaatt
    13321 tgattttttc agtagaggtt ccggaaattt ttcagacaac tttagacaag ttgagaaaaa
    13381 attaagtttt tcttgaaaaa tacttttacc atttgctata atataagcaa atcattcctt
    13441 taaccttatc cagtgagata gttaaggaga aacttcagac taaatcaatg cctgagcggc
    13501 tttgtcattc tagagagtct ccttttgaag gggctctttt atcttagaga ggatactgca
    13561 atgaaaagaa aagaaattat tgatgatgtg accatgaaac gtgctattac acgtattacc
    13621 tacgaaatta tagaacgtaa caaaaattta gataatattg ttttagcagg cattaaaaca
    13681 agaggtgttt ttttagccaa acgcattcaa gaacgtttga agcaattaga aaatttagat
    13741 attcccgttg gagaattgga tactaaacct ttccgtgatg atatgaaagt tgaagttgac
    13801 accacaacta tgccagttga catcactgat aaagatatta tattaattga tgatgttctc
    13861 tatacaggtc gcactattcg agctgctatt gataatcttg ttagtttggg gcgtccgagt
    13921 cgtgtcagtt tagctgtatt aattgaccgt gggcatcgtg aactacccat tcgtgcagat
    13981 tatgtgggga aaaatattcc aacaagtcaa tttgaagaaa ttctagttga agtgatggaa
    14041 catgatggtt acgatcgtgt gagtattatt gatccgagtt aagtagacga ctattattta
    14101 cgatgatttt atagaagggg aagaaccaca atggcaaaaa aattgttaat tctagaggat
    14161 ggtacggttt ttgaaggact atcctttggt tcatctcttg atgtaactgg cgaattagtt
    14221 ttttgtacgg ggaataccgg ctatcaggaa ataataacga atccttcaca taatggaaaa
    14281 attttagttt ttacctcacc gctaatagga aattatggta ttcatcgtag ttactctgaa
    14341 gccattattc ccacttgttt aggagttgtt gttgcagagt attccagatg tgtaagtagc
    14401 gatactagca agatgaatct agatgaattt ttgaaaatga aaaaggttcc tgcaatgtcg
    14461 ggggtcgata caaggtattt gatgcaagtt attaaagaaa aaggatttgt aaaagctact
    14521 ttagcagaag caggtgatgt tttatcacat ttacaagatc aattaatagc gacagtatta
    14581 ccgacaaata atgtggaaca agtttcgaca aaaacagctt atccgtcgcc tgctagcggt
    14641 cgaaatattg ttgtacttga ttttggcctt aaacattctg ttttgagaga attgtctaaa
    14701 cgtcagtgtg acgtgactgt cattccctac aatacgtctt tagaaggtat caaaaatcta
    14761 tacccagaag gtatcatatt atctaatggt ccgggaaatc cagaaaagct tcaggaagtc
    14821 ttaaacacta ttaaagaatt acaaaaatct gttcctatgt taggtattgg tttggggcat
    14881 caattaattg ccatggcaaa tggtgcagaa attatgagac tcccagttgc taagaagggg
    14941 ccaaattatc ccatgagaga tattgctacg gggcgtttag aaacagttat tcagtttaat
    15001 cattttaccg ttaaccggct aaatcttccg catgatttac tggtaacaca cgaaggtttg
    15061 aatgatcaag agattgttgc tctaaggcac cgctcatttc cagtgatgtc cgtgcaattt
    15121 tacccagagg ctgctccagg ccctcatgat gtgacctatt tttttgatga atttttagaa
    15181 atgattggat cacgcgctta aacataaggt attatttaaa gtcagtcggt gagctgatta
    15241 ataaggaggt gcgtatatgc taaatacaaa ggataaacag cctattttgc ttattatttt
    15301 cggacagaaa atgaaagaat atgacgattt aattagatca actgttgaga ggttatctaa
    15361 aaggaataga cctgttagta ttcttatctc gaaggagcta aaccatgtga ttcaccatta
    15421 tgatgcacaa tttgaaaatc ttgagacgct tgtggggtat ataaatacac atgaagatag
    15481 tttgatttat cccctttgct tagataaaaa tcatgagcta tttcaagagt ttttagcgtg
    15541 gcagagtcat tattcaaggc agtatatcag tgataattta tcagaaaaaa gtgttcaggc
    15601 aatagacaat aatcaagact ttgtgcaagt agattgttta gtaatgagag actctttgaa
    15661 taattgttta tatgtctctg atttagaata tattgaatct aataaaacaa ctggtaagtc
    15721 tctagcgatt gtaccaagtc aaactttatc cgatgctgct aggcaaacaa ttcgagatgt
    15781 tgcatttgat gtgtgtcgaa aggcaaacat tattggagta tgttacttta gtttcttaat
    15841 agatttgaac agtttggatt atcatataat tagcttatca tctggtttgt cttatcaaag
    15901 cattttattt gagactatta cgacctatcc tgttttagag atagcaacta aattgacagt
    15961 gggatatact tttagtcaac tgaagcattc ttattaccca aatacctctg cctttttaga
    16021 gccgcaatta gactatgtag ctaccgtatc ttttagtttt gaaaaagttg actatatctt
    16081 ttttgctcgt aatatagagc aattattttt aaatttactc gaggctagca gtcatgatca
    16141 ttttccattt ctgagtgata ttagcgagga agatttgatg tttgctttga ttcagaaaaa
    16201 agaaaatcgt ctagcttatc ttttagaggc ttttagacgt ggctttgatt tgtatgattt
    16261 atcctcagtt actaaaatca acccttttta cttagataaa tgtttacata ttgtagagtt
    16321 gtatgaaaac ctgaataaaa gccaatataa tgtagatatt tacaaggatg ctaaaaggta
    16381 tggtttttcg gatgactata ttgctagtag ttggcaaata tctttgatag acatgttaga
    16441 atatcgaaaa aaacatagtg tcgcacctgt tctgaagcaa gtcgaacaaa gttctggtgt
    16501 tctaactggc catcaaattc agtattttcg aagctatgat tggcattcag actacagttc
    16561 tagcggttgt caaaaggctt tgataatggt agacaagggt tattccctag taaaattaaa
    16621 tgagttaatt aagcaaatca aacaaactca tttagaatta ttaattgtta ccaatcaacc
    16681 tctcctcata gaacaattaa atgatacatc tataattttt gacactatag gtattgagac
    16741 catattaaca attatgggta tagaagaggt ggaagaagtg tatttattgg gagaaagtca
    16801 agcagacatg ttttcaaaaa tgaaaaattt ttctatctct tgtcagtcta tttagcaatt
    16861 cttaataaaa gctgagatat agatctcagc ttttatttgt ggtaaaaagt aaagtacttt
    16921 caattttgta agttctttct atgagcaaaa tagtatactt aatgagtaaa aatattattt
    16981 ttaaagaatt ttcttgacaa tattatttaa aaataataca cttgttctat attaataata
    17041 caaacaggag atggacatga gtaaacgaca aaatttaggg attagtaaaa aaggagcaat
    17101 tatatcaggg ctctcagtgg cactaattgt agcagtaggt ggctttttat gggtacaatc
    17161 tcaacctaat aagagtgcag taaaaactaa ttacaaagtt tttaatgtta gagaaggaag
    17221 tgtttcgtcc tcaactcttt tgacaggaaa agctaaggct aatcaagaac agtatgtgta
    17281 ttttgatgct aataaaggta atcgagcaac tgttacagtt aaagtgggtg ataaaatcac
    17341 agctggtcag cagttagttc aatatgatac aacaactgca caagcagcct acgacactgc
    17401 taatcgtcaa ttaaataaag tagcgcgtca gattaataat ctaaagacaa cagggagttt
    17461 tccagctatg gaatcaagtg atcaatcttc atcatcatca caaggacaag ggactcaatc
    17521 gactagtggt gcgacgaatc gtctacagca aaattatcaa agtcaagcta atgcttcata
    17581 caaccaacaa cttcaagatt tgaatgatgc ttatgcagat gcacaggcag aagtaaataa
    17641 agcacaaaaa gcattgaatg atactgttat tacaagtgac gtatcaggga cagttgttga
    17701 agttaatagt gatattgatc cagcttcaaa aactagtcaa gtacttgtcc atgtagcaac
    17761 tgaaggtaaa ctccaagtac aaggaacgat gagtgagtat gatttggcta atgttaaaaa
    17821 agatcaggct gttaaaataa aatctaaggt ctatcctgac aaggaatggg aaggtaaaat
    17881 ttcatatatc tcaaattatc cagaagcaga agcaaacaac aatgactcta ataacggctc
    17941 tagtgctgta aattataaat ataaagtaga tattactagc cctctcgatg cattaaaaca
    18001 aggttttacc gtatcagttg aagtagttaa tggagataag caccttattg tccctacaag
    18061 ttctgtgata aacaaagata ataaacactt tgtttgggta tacaatgatt ctaatcgtaa
    18121 aatttccaaa gttgaagtca aaattggtaa agctgatgct aagacacaag aaattttatc
    18181 aggtttgaaa gcaggacaaa tcgtggttac taatccaagc aaaactttca aggatgggca
    18241 aaaaattgat aatattgaat caatcgatct taagtctaat aagaaatcag aggtgaaata
    18301 atcgtgacaa ctaatcgaaa agaactgatt agattgcatc agattgttaa aagttatcaa
    18361 aatggggatc aaaaacttca agtcttaaag aacattgatt tgacggttta tgaaggtgaa
    18421 tttcttgcaa tcatggggcc atctggctca gggaaatcca cactaatgaa tattattggc
    18481 ttgttggatt ccccaacttc aggagattat agtttaaatg gtaaacgtgt cgaagaacta
    18541 agtcaaacaa aattagctca agttcgaaat aaggaaatcg gttttgtctt tcaacaattt
    18601 ttcttgttat ctaaactaac ggctttgcaa aatgttgaat tacccttaat ttatgcgggt
    18661 gtagctccta aaaagcgtaa aaatttagca aaacaatttt tagataaagt tgaattgaga
    18721 gagcgtatga accatctgcc aacagaatta tctgggggac agaagcaacg tgtagcaatc
    18781 gcgcgtgctt tggtgaatag cccatctatt attctagcag atgagccgac tggtgccttg
    18841 gatacaaaga cgggtgagca aattatgcag ttcctaacgg aattaaatca agaaggtaaa
    18901 accattatca tggtaactca tgaaccagaa atcgccgatt atgcgacacg taaaattgtt
    18961 attcgtgatg gcgaaattac agcagatact actgatagta ttagaattga ttaaggggag
    19021 attcgattat ggaaaattgg aaatttgccc tatcatcgat tctagggcac aagatgcgtg
    19081 cttttctgac catgttaggg attatcattg gggttgcatc agttgtgtta attatggcct
    19141 taggtaaggg catgaaagat tcggttacaa atgagattac aaagtctcag aagaatcttc
    19201 aaatttatta taaaacaaaa gaagaccaaa aaaatgagga taactttgga gctcaagggg
    19261 ctttcatgca aggtagtgat accaaccgta aggaaccaat tattcaagaa tcatggttaa
    19321 aaaagattgc taaggaagta gatggtgtta gtggctacta tgtaaccaat caaacaaatg
    19381 ctccagtagc ttaccttgaa aaaaaggcaa agacggttaa tataacagga attaaccgca
    19441 cttaccttgg tatcaaaaag ttcaaaatta aaagtggtcg tcaattccaa gaggaggatt
    19501 ataaccaatt ctcgagagtt attttactag aagaaaaatt agctcagaga ttatttcaga
    19561 caaatgaagc agcgttaaat aaagtggtga ctgtaaaaaa taaatcttac ttagtagtgg
    19621 gggtttattc ggatccagaa gctggctcag gtttatatgg aagtaattct gatgggaatg
    19681 ctattttaac caatacacag ttagcttcgg agtttggtgc taaagaagca gaaaacatat
    19741 attttcatct taatgatgtg tcgcaatcca atcgcattgg taaagaaata ggcaaacgtt
    19801 taacagacat tagccatgct aaggatgggt actatgataa ttttgatatg acatctatag
    19861 ttaaatctat caatacccaa gttggtatta tgacaggtgt tattggagcg attgcagcaa
    19921 tttctctcct agttggtggt atcggagtga tgaatattat gttagtctct gtaactgagc
    19981 gcacgagaga aattggcctt cgtaaagccc ttggggcaac tcgacgtaaa attttagctc
    20041 aatttttaat tgaatcaatg gtcttaacga ttttgggtgg gttaattggg ttattactag
    20101 cctatggagg tacgatgtta attgctaatg cgcaagataa gattactcct tctgtgtcac
    20161 ttaacgtggc tataggcagt ttaattttct cagcatttat tggaattata tttggtttat
    20221 tacctgctaa taaagctagc aaattaaacc caattgatgc tttacgatat gaataaaaaa
    20281 acatgtactc tgtcaagatt ttttcttgac agagtttgta tttatgttat acttagtaag
    20341 gtaaagttgt tagttaagct atcaacgacc aaatataaag aaaaagaaaa gagactattt
    20401 aaaaatggca gtaaaaatcc gtttaactcg tatgggttct aagaagaaac ctttctaccg
    20461 tatcaatgtt gcagattcac gtgcaccacg tgacggacgt tttattgaaa ctgttggaac
    20521 atacaaccca ttagttgcag aaaaccaagt tacaatcaaa gaagaacgtg ttcttgaatg
    20581 gttatcaaaa ggtgcacaac cttcagatac agttcgtaac cttctttcaa aagctggagt
    20641 tatgactaaa ttccacgatc aaaaattctc aaaataataa aagcctatgg ataccattga
    20701 aaatctcatt attgctattg tgaaaccttt gatttcacaa ccagatcagt taaccataaa
    20761 aattcaggat ggcccagaat ttttagagta ccatcttgat ttagataccc aagacatcgg
    20821 tcgtgttatc ggcaaaaagg gtcgtacaat tacagcgata aggtcgattg tctattcggt
    20881 accaacacaa gggaaaaaag taagactcat cattgatgag aaagaataat ttattctaaa
    20941 agataggaac ttcctgtctt ttttgttata tctacacaaa aattgtgagt tttttagtct
    21001 tttagaatat aatggatgtg aaagggaatt ttctgatttt ttattaagga ggataagata
    21061 ttgctatttg attttctcga agaaaaaata aaactgaaag catacctgat gaccgcctta
    21121 cttgatgatg gagaaattaa ccttgatcac tttaaaacag aatatgcttt gagtgaaaag
    21181 attttacaag gtcttatttc ggaactacaa atactttatt ctgatttcta ccttgttgtc
    21241 aaaagtagaa caatggtatt gttccattac gaaacacttt ctaagctaga gactttgcat
    21301 accatttatc gagagtctaa tacgttacag tttttggcat attttattgc tcctcaaaat
    21361 gtcagttttt ctgaattcac ggctaagaaa ttcatatccg tcgcaagtgc ctaccgaata
    21421 aagaaaaact gtaaacttta tttggagagt gttaagttat caatacaaaa aaataaagtg
    21481 acaggtccag aatatcagat taggctgctg attgctttgt tacagtatca ttttggtttt
    21541 attatttatc cttttgatgc tacaagtgag gtaatgataa gggattttat tgaatactcg
    21601 aatt
//
LOCUS       DATRSG010000032        21069 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRSG010000032 DATRSG010000000
VERSION     DATRSG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21069
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.032"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..632)
                     /locus_tag="VBD89_001169"
     CDS             complement(<1..632)
                     /locus_tag="VBD89_001169"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7135707.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVG"
     gene            complement(699..1370)
                     /gene="deoC"
                     /locus_tag="VBD89_001170"
     CDS             complement(699..1370)
                     /gene="deoC"
                     /locus_tag="VBD89_001170"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO7135708.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVDEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            complement(1400..2602)
                     /locus_tag="VBD89_001171"
     CDS             complement(1400..2602)
                     /locus_tag="VBD89_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO7135709.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGTKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILFPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            complement(2623..3402)
                     /gene="udp"
                     /locus_tag="VBD89_001172"
     CDS             complement(2623..3402)
                     /gene="udp"
                     /locus_tag="VBD89_001172"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO7135710.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            3561..4298
                     /locus_tag="VBD89_001173"
     CDS             3561..4298
                     /locus_tag="VBD89_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7135711.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYHEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            4311..4675
                     /locus_tag="VBD89_001174"
                     /pseudo
     CDS             4311..4675
                     /locus_tag="VBD89_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4707..6329)
                     /gene="groL"
                     /locus_tag="VBD89_001175"
     CDS             complement(4707..6329)
                     /gene="groL"
                     /locus_tag="VBD89_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO7135712.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(6425..6709)
                     /gene="groES"
                     /locus_tag="VBD89_001176"
     CDS             complement(6425..6709)
                     /gene="groES"
                     /locus_tag="VBD89_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO7135713.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDTTKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            complement(6884..7687)
                     /locus_tag="VBD89_001177"
     CDS             complement(6884..7687)
                     /locus_tag="VBD89_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7135714.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGTITLDNQSITKDSVEKRSKGISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            complement(7692..9566)
                     /locus_tag="VBD89_001178"
                     /pseudo
     CDS             complement(7692..9566)
                     /locus_tag="VBD89_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364177.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(10031..10828)
                     /locus_tag="VBD89_001179"
     CDS             complement(10031..10828)
                     /locus_tag="VBD89_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7135715.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            11014..11874
                     /locus_tag="VBD89_001180"
     CDS             11014..11874
                     /locus_tag="VBD89_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7135716.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQENRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            11917..12648
                     /gene="yaaA"
                     /locus_tag="VBD89_001181"
     CDS             11917..12648
                     /gene="yaaA"
                     /locus_tag="VBD89_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO7135717.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFKTVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTSMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(13005..13622)
                     /gene="nrdG"
                     /locus_tag="VBD89_001182"
     CDS             complement(13005..13622)
                     /gene="nrdG"
                     /locus_tag="VBD89_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO7135718.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDYLL"
     gene            complement(13695..14186)
                     /locus_tag="VBD89_001183"
     CDS             complement(13695..14186)
                     /locus_tag="VBD89_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7135719.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTTHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGILEDCRHQTERY
                     WIT"
     gene            complement(14195..15127)
                     /locus_tag="VBD89_001184"
     CDS             complement(14195..15127)
                     /locus_tag="VBD89_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7135720.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(15140..15283)
                     /locus_tag="VBD89_001185"
     CDS             complement(15140..15283)
                     /locus_tag="VBD89_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135721.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(15358..17556)
                     /gene="nrdD"
                     /locus_tag="VBD89_001186"
     CDS             complement(15358..17556)
                     /gene="nrdD"
                     /locus_tag="VBD89_001186"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO7135722.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYENVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(17655..19196)
                     /locus_tag="VBD89_001187"
     CDS             complement(17655..19196)
                     /locus_tag="VBD89_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135723.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLFIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLL
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(19447..19764)
                     /locus_tag="VBD89_001188"
     CDS             complement(19447..19764)
                     /locus_tag="VBD89_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO7135724.1"
                     /translation="MAHNHNHGHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(19790..20209)
                     /gene="ruvX"
                     /locus_tag="VBD89_001189"
     CDS             complement(19790..20209)
                     /gene="ruvX"
                     /locus_tag="VBD89_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO7135725.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQVYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(20209..20475)
                     /locus_tag="VBD89_001190"
     CDS             complement(20209..20475)
                     /locus_tag="VBD89_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO7135726.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(20677..>21069)
                     /gene="spx"
                     /locus_tag="VBD89_001191"
     CDS             complement(20677..>21069)
                     /gene="spx"
                     /locus_tag="VBD89_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO7135727.1"
                     /translation="KIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILEIL
                     TKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQVG
                     YKEDDIRAFLPRSIRNVENAEARLRAAL"
BASE COUNT         6750 a         4288 c         3209 g         6822 t
ORIGIN      
        1 tcacctacat atggacgagc gataatacga ccaaggagag ctggacgctc cattgtgata
       61 ctacgagcat attcacaaat acggtaaagt tcttctagag ggatgatatc ctcatgagct
      121 gcaatttgta ggactgggtc agctgaggta tagatgatga gctcaccagt ttccatttgg
      181 cgtggtccga agtcatcaat aacagctgtc cctgaataag gtttattcgc ttcacgaata
      241 actttacgtc ctgaaaaatc ctcaatcttt gtgatgatat cttctggaaa accattccag
      301 aaagtatcaa atggctctgt aatatttaag cccatgatct cccaatgccc tgtcattgta
      361 tctttaccaa gtgatacttc ttgtaacttc gtcgcgtagc cacttggatt ttcttctgca
      421 ggaacggttt taagcgcttg gggacgagga atattaccaa gtccaatttt agccatattt
      481 ggcaccgcaa gtccaactgt ttttgaaatg tggccaagcg tgtctgaagc accatcagga
      541 actcctgcat tgacaaaatc attggcatct ggtgccgctc caatacccac tgaatctaag
      601 acaactaagt gaatgcgatc aaattgtgac ataacttctc ctttatcaat tgaaaatagg
      661 aagtcagacc agcatccttc ctatttcctt tatattattt aataagtgcc ttcttcaact
      721 ttttggttct taacaatctt gatgatacgg ctagtaccca agcgtgaagc tcctaaagca
      781 ataaattttt cagcatcttc caatgatgag attccacctg ctgccttaat tttaacacct
      841 tcgtcgacat attttgccat cacttcaaca tcttcaaatg tagcacctgc tgtcgaaaaa
      901 ccagtagagg ttttaataaa gtctgcacct gaacgtgtga caactccaca aagttcgata
      961 agttcttctt tagttaattg acatgtctca acgataactt ttaagatatg gtcttgacat
     1021 gcagctttga tttgacgaat ttcttcttca acagtatcaa aatccccatt tttaacgtct
     1081 gtcaaattaa tgaccatgtc aatttcatca gcaccatttt taatagcatc ttgacattca
     1141 aaaaccttcg ctgcagttgt actatagcca tttgggaacc caataacagt acaaatagct
     1201 aatttacctg aaacgtactc tgctgctttt ttgacgtaag aggctggaat acatgctgaa
     1261 gctgtttcat aagccattgc atcatctaaa attgtttgga tttctggcca tgttgctgtt
     1321 gttgctagca aagtatggtc taccgttttt aaaatatctt ttacttccac gttactatcc
     1381 ttctttcttt tgtcaacttt tagaatagag ttacaatacc gacgataaag gcgctaagca
     1441 tacttacagc aataccacca atcattgcac ggaaaacaag tcgagctagg gtgccacgtt
     1501 tctctggaca aagaacagca atacctgaaa cacaaatacc taaacttgat aaattagcaa
     1561 aaccacagag tgaaatagtt gctaccaatg ctgtacgata atctaaagat ttgattaggt
     1621 gacccaattg ttggaacgaa acaaactcat ttaaaatcaa cttacttcca agaaggtttc
     1681 cttctagaag aatgtttttg tggtcaaatc ccataagaaa accaaatgga gcaaaaacat
     1741 atgagaagat ttgttctaag cgaattccca atccacttaa catcatatta atcaaagaaa
     1801 ctaaaccaac aaaggcaatc aaactagcac caattgagaa agccatttgt gcacctgtgc
     1861 ttgcaccctc agcgattgca tcaatcacat tggcgttatt acctttatta tccatcttaa
     1921 tgtcatcaat tttttgaaca ggttctgttt gaggaaataa gattttagca atgagaatac
     1981 tgccaatagg aaccattgtt gaagcaatca agagatattc cattggaatg cctaatgcaa
     2041 tgtagccacc aagaatagaa actgacatac ttcccatacc tgataccaac acaaccatta
     2101 tctcactatc agtcatacga cctaggtatt tgctaaccaa aattggactg tctgtttgac
     2161 caagaaacat attagctacg gcaacaaaac tctcaacctc tgaggatttc ataattttac
     2221 caacaccctt acctatccat tttactacaa atccaaggat tcctacataa taaagtagac
     2281 taactagggc agataagaaa acaatattac caagcgtttg aatggcgaaa ataaaaccag
     2341 tttttgttcc actatctgct aatgaaccaa acacaaaatt taaaccagct tgaccacagt
     2401 tgattacttt agtaactcca gttgaaacaa cactaacaac ttgttggcct agtgggatac
     2461 gtactaagat tagcgcaata atgaattgaa cgataagagc ttttccaatt aaacttagag
     2521 aaacactctt acgattgaaa gaaattgcat acacaattcc taataccaat aaaataccaa
     2581 taatactata aataaattgc atgatgactt ccaaaacctc gcttattgtg atttatcatt
     2641 ttcaataagt gtgcggagtg cttcaacagc aacttgaata gctgcttctg tgtcatgagc
     2701 cattgggtta tccataccca aagcgttgcg ttcttgatta ccaacaacaa ggaaatctga
     2761 tccacaacga actcctagat gacttgctgc tacaaaaaga gctgcagatt ccatttcaga
     2821 cgcttttgtt cccagacgtt tccatgcttc ccatttattt aataattcat aactaactgg
     2881 catacgttct ggctcatgtt gtccataaaa tgcatcttta cattgaacta ctcctgcgtg
     2941 acttgtgtaa ccaagctttt tagctgcatt aactagagca ttcgttacct ccaagtcagc
     3001 tacagcagga aattcaatcg gagcatattc tttacttgta ccttccatac ggatagctcc
     3061 agttgcaata acgatgtctc cgcctttaac atcaaggtca ataccaccac atgttccaac
     3121 acggataaag gtatcagcgc cacagagttt caattcttcc atcgcaatag acgctgatgg
     3181 accaccaata ccagttgaag taacactgac tttttcacca tttaatgtac ctgtataagt
     3241 aacatattca cggctgtctg ctacaagaac tgcattatca aaatgttctg caatttttgc
     3301 acaacgtttt ggatcacctg gcatgataac ataacgacct acatcacctg gacgaatctg
     3361 taggtgatat tgcaatccga cttcacctga ataattctgc atgataaaga cctccttaat
     3421 gtcttgtcta ttttttttac acttgtataa taacatacaa gacaaccttt gtaaacgctt
     3481 ttatcgaaag aaaacgtttt attttttaac ttgatttttc taattttttc aattaaacta
     3541 acttgtatac ggaggttaca atgcctaaaa atgaactaaa taataaacta aataaactaa
     3601 agcatgtgca agtctacaat aagattttta acatgattca agacggtact tattctcctg
     3661 gaatgcaatt gccttctgag cccgaattag cagggcaact taatgttagt cgagctactt
     3721 tacgaaagtc attagcacta ttacaagaag accatttggt taaaaatatc agaggaaaag
     3781 gtaatttcat tagagaaaat tctagcaact tgtctgaaaa cggttacgaa aatagacaac
     3841 accccattaa aacttgtcta acttccaaaa ttacagaagt ggaattagaa tttcgtgtag
     3901 aggttcctgc agaagctatc acagcttcac ttaaacaaga aacccctgta gttgttattg
     3961 ctgatcgttg gtaccacacc gatgatggac cattagctta cacactttct tttatcccta
     4021 tcgaacttat ttcagatgct gagatttctt tacatgatac taaacagttg ttaaacttca
     4081 ttgaagaagg tatctatcac gaaggcatat caagccactc acaaagccat ttgggatatg
     4141 cgacatctgg caatttttca gcaactaaat acaccctatc cgatcatgga caattcatat
     4201 taatacaaga aacaattttt aaccaagaga aaatactaat gtgtaataag cattacgttc
     4261 ccattgagca ctttgaattg tccattacct ctcattaagg aggcaagcct atgcaaaata
     4321 gactattaaa agttgctttt ttcataactt ttaccgaatt aattatgggc ctaatagttg
     4381 gcgcttattt taagttaagt tattttctag cccctccaaa ggaaccgtca atgggtgctg
     4441 ggtacggaat gctcttttct atgttattta tacctattag ttacttaatt ctattagcta
     4501 taattcaatt cttagcttat caatctcaat ctaagataat agcaatcatt attttgctac
     4561 ttttttcctc ccactactgg caaatattag atggcctatc cttatgatct tcttaccgca
     4621 agtggtagtg actatcatat ctctattatc acaacataaa actccccttt gttaaagggg
     4681 agttttatgt tgtgataagt tagtgattag aagccaccca tcatagatgg atccattgca
     4741 ggagctgtag gagcttctgg ttcaggttta tttgctacta ctgcttctgt agtcaagata
     4801 agacttgcta cagatgccgc attttgaagt gcagaacgtg ttactttgac aggatcaatg
     4861 atacctgtgg taaccatatc tacccattct ccattagccg cattaaatcc tgtaccaatt
     4921 tcagactgtt ttaaacgttc aataataact gaaccttcat atccagcatt gtaagcaatt
     4981 tgacgaacag gctcttcgag agcacgaaga acaatattac gtccagtctc ctcatcacca
     5041 ttaagtttca gtgccgctac tttttcaata acgttcacaa gagccgtacc tccacctgaa
     5101 acaatacctt cttcaactgc agcacgcgtt gcatttaacg catcttcgat acgaagtttc
     5161 atctctttta attctgtttc agtcgccgca ccaactttaa ttactgctac accaccggct
     5221 aacttagcaa gtcgttcttg taatttttca cgatcaaaat cagaagttgt agcctccatc
     5281 tgtgacttaa tgatggctac gcgattagca attgctgatg agtcaccggc accttcgaca
     5341 ataacagtag aatctttatc tactgttact ttagcagact gtcctaaaac ttgcatagta
     5401 gcatctttta agtctaaacc aaggtcttca gtaacgacag ttcctcctgt taggatagca
     5461 atatcttcca gcatggcttt acgacgatca ccaaatccag gcgctttaac agctacgaca
     5521 ttgaaagttc cacgaatttt gttaagaaca agcgttggga gagcttctcc atcaacatca
     5581 tcagcgatga ttagcaacgg acgatttgtt ttaagaacct cttctaataa tggtaaaatt
     5641 tcttggatat ttgaaatttt cttatctgta ataaggatat acggattctc aagttcagag
     5701 accattttct cgttatcagt taccatatac tgtgacaagt acccacggtc aaactgcatt
     5761 ccttccacaa cttcaagctc tgtttccata cctcgcgatt cttcaatagt gataacacca
     5821 tcattaccca cgcgctccat agcttcagaa atgtattccc caactttttc agaacgtgaa
     5881 gacacagccg caacttgagc aatagcttct ttgcctgaaa ctggttgtgc aatctctttt
     5941 agctcttcaa ctgctgctga aacagctgtt tcaataccac gacgaatgcc aatcggattt
     6001 gcccctgcag ttacattttt aagaccttcc cgtacaatag cttgggtcaa aacagtagca
     6061 gttgtagtgc catcccctgc aatatcatta gtttttgaag ccacttctga cacaagttta
     6121 gctcccatat tttcaaagtg atcttctagc tcaatttctt tagcaattgt cacaccatca
     6181 tttgtaatta aaggagaacc aaatgctttt tcaagaacaa cattacggcc tttaggacct
     6241 aatgttactt tgactgtatc agctaaaata tcaacaccac gcaccattgc tgagcgggca
     6301 tctgctgaaa atttaatatc ttttgccata atttaatacc caggcctaaa atttaacaaa
     6361 gtctactttc tttacaaagc aacttttctc taataggcct atatcctttc ttgcttaata
     6421 aaacctattt aacgacagca acaatgtcac tttcgcgaac aactgtcact ttttcgttac
     6481 cgtctttaac ttccaaacca gcaccatttt cgactaaaac agtatcaccc tctgcaacgc
     6541 taggtgcaac taattcccct gttaaggtac gaataccgtc acccacagca agaactttgg
     6601 ctgtctttgt cgtatcatgg cttgctcctg caagaacaaa accaccgaca gatttctctt
     6661 cagtttcaac aaacgaaatg atcacacgat cacctaatgg ttttaacata ataaacgaac
     6721 ctccatacga gatatatttt gattttttag cactcctata aagagagtgc taactacaaa
     6781 aactattcta tcacttggtc agaattagtc aagagaaaag gcacgtttca aacaagaaaa
     6841 atttccctag ttatcctgtc cattcactag agaaatttat attttaacct aagacaaggg
     6901 catcatctat caactgttga cctgagtttt tatggaaaag ttccattagt tctgcaactg
     6961 ttaagttcct ctttgcttct cctttaacat caacaacaat tttcccgtgg tagagcatca
     7021 cgagacgatt accataagca atggcatgct ccatattgtg tgtaatcatt aaggctgtca
     7081 atcgttgttc ttctatcact ttctgtgtta attccataac catatcgcta gttttgggat
     7141 ctagagccgc tgtatgttca tccagtaata ataatttggg acgaacaaga gttgccatag
     7201 ctaacgtgag ggcttgacgc tgaccgccag ataaaaatgc agcatcagtc ttcattcgat
     7261 tttctaagcc tagacctaat tgtgatagag actttttaaa tagttgacgg tcatcgtcag
     7321 tcactgactg tctaaaaatg tgacgcttat tgcctctttt atgtgcaatt gccatatttt
     7381 cttcaatcgt caaatttgtt gcagttccca ttctaggatc ctgaaagact cgactaatac
     7441 ccttagatcg tttttctaca gagtctttag taattgattg gttgtctaat gtgattgtcc
     7501 cctgatcaat agggatcaga ccagcgatac agttaaggag ggttgatttt cccgcaccgt
     7561 tacctccaat tactgaaata aaatcaccgt cttcaatcgt taaatctaaa ccacgtaaaa
     7621 catggttctc attgacagtt cctttttcaa aagttttgtg cagattgact aattcaagta
     7681 atgacatgtt cttatccttc ctttgtaagc gttttagagg cttttactcc tagtttagct
     7741 cgtaattctg ggacatacaa aatgagagcc aacaaaattg ctgaaaccaa ctttatcatc
     7801 tgtgcatcaa tatcggttgt taatatgaaa acaataatca tacggtataa aacagaacct
     7861 aatacaattg accataacct tttaccaaga ggtaagtatt ttatcattac ttctgccaag
     7921 ataattgaag ccaatccaat aacgattgta cccactccca tattcaaatc tgcatagcca
     7981 ttattttgcg ctaaaagtgc accagataaa gcaatcaggc cattcccaat catatatcct
     8041 agcattttca tccgatctac tttaatacta ttggcttgtc ccatagcctc attatcacct
     8101 gtcgctctta aagctaatcc tagttgggta tttaaaaaga gatacaaaat taagataact
     8161 aaacctacac aaacaattcc aattaataaa actgctgaaa gtttatttaa acctaatctt
     8221 gttaccattg taaccaaagt atttttgaga gcaaaagaga cgtttgatct tcctaaaaca
     8281 agtaaattaa tagagtaaag tcctgtcaac gtaataatac ctgttaggag ggctggtatt
     8341 ttcattttag tatgaagaaa acccgatact agtcctgcca acataccacc taacatgccc
     8401 gctatggttg ccacaattgg attaatatca ttaacgatac ataaggcgca aactgcagcc
     8461 cccataggga aagccccttc tgcagagaga tcggctatgt ctaagatacg aaaagtgata
     8521 aacacgccaa ttgccataat tgaccaaagt aatccttgcg aaattccgga taagataata
     8581 tctaacatac aatacctttc tgattattta ttatttttaa tacttgaaac atcaattcca
     8641 agctcttttg ccatatcttc attaactact acttttagat tttgaggata ttctgaagga
     8701 actttagcga cgtctttacc tttgaggatt ttaacagctt gtcttgccgt ttgacaacct
     8761 aaagcctcat aatcagcccc ataagaaaag agcaaaccac ttggtaccat atctgctgaa
     8821 cctcccacaa caggcacttt taattctttc gatagattcc ctaacagcgt tactgagctg
     8881 gctataatat tatctgttgg tacaaaaaga acctctgttt tagacattaa actcttagca
     8941 gtatcttgta catcattagt ggaagaaata cctttaacac ttgttttaat ccccgcctct
     9001 tggaagattt tttttgcttg ttttacctga acttctgagt tacgctcgct agtggtatac
     9061 ataataccaa cacgtttaac cttaggcatt acttttctca aaagggatac ttgcttttta
     9121 attggagaca tgtcacttgt accagtcgct aaaccttcag gttttttcat tgattttact
     9181 aattcggctg ataccggatc tgtcactgct gtaaataaaa taggtgtttc tgttgacacc
     9241 gttgtcaacg attgagccgc cggagtcgca attcccaata ctaatttatt atctttaatg
     9301 agtttttcag aaattgattg gatatttgac tgatcccctt gagcattctt atactcaatt
     9361 ttgatatttt tgccatcttt atagccttct ttagctaatt cttttataaa tcctttccta
     9421 gtggcagtta gagagggatg ttctacatat tgtaaaatac caatatgttt tacatcagat
     9481 tgaccatttt gagaagattg tgaacaagct gtcatcagaa cagatgctaa aacgctgcta
     9541 attaccatct ttgttatttt tttcattact atctccttgt aataccatca aattttccat
     9601 aaaacaaaaa accaactaga ccctacatct agttggttaa ttatttctct caattcctct
     9661 gtgcacggga gggcgaaaaa atattgcctt aaccaactag attattgaaa tgatctaatc
     9721 ggctctgctt tcatcataaa atgctttagc ttcatagata caaactttgc gtgacgtttg
     9781 tatccttgag gctacaaacg tctatctaaa gaaactaaag taataataag tatttgtttg
     9841 tttaataata gacgatttga ttaacatatt attctctcct tgtttatggt tgcctataag
     9901 tttacgtcat ttaaaaataa ttgtcaatag ttaaatttga tttttttgaa aattcttgtt
     9961 agatagataa tttaaataat agtcattcgt tgatataaca cacttttaag gaaatttaga
    10021 tttgctatcc ttaattttcc atatactcta ctaaagcata ataaatacca tcgttatcat
    10081 tatcctgagt tacatttcca attctctctt gtaaatcttt aggagcattc cccatgacaa
    10141 aacctttacc cacaatttca agcagattta aatcattata gttatcacca aacgcaatag
    10201 agtctgcaag agaaatatct aaatagtctg ctaaagtttt agcagatttt cctttttcaa
    10261 tacctggagc cattatttca ataaagtaag gagcagattg tgcgattgat aaatgtggat
    10321 attgagcttt caaagtgttc tccaattctc ccatcagaga tggttctccc atcaagagaa
    10381 gtttatgaat cctatcttgc ttctctaact ccatcaaact catctcttta gattgtaaac
    10441 caacaatctc ctcctctttt tggacccact cattttcttt atcacaggaa taccattcat
    10501 gataacgata gacattccat gcaattttcg aataatgctt gcttacataa gaacaaactt
    10561 gtaaggcaac ttgaccatcc attgtgtggg aagcaattgt tttttgttgc atatcttgta
    10621 ttaaagcccc attataggaa ataatataag aatctattcc aatttgttcc ataatcggag
    10681 taattgcctc aggcatccta gctgatatgg gaacgaaagt agccccttga gctactattt
    10741 gtttaattcc ctgccgtgtc ttaggtgtta ctttaaaatt actgttaatt aaagtaccat
    10801 caatatcact aaatattagc cttaccatat tatcctccaa aatataattg tacgcgctta
    10861 ctttagtcta acacttaaat ttttgtttcg caaataaact tcgtctggtc taatgagact
    10921 actaatataa gctatatttc aaaacaaatt agtttatttc tctcaatgaa cggtttaata
    10981 tgttactata tagtaataat aggaggatta actatgccgt atccatacaa agccaatcat
    11041 tccattgaat caatcacctt aaaagtaaat gacctcgaaa acctcgtaaa cttctatagc
    11101 gatatcattg ggttaactgt tattgacaaa tcaagcacta gagccttact aggggtaaat
    11161 caaaaaatcc ctcttattat ccttgaaaaa actgaacttg aaaagcatag tacctatggc
    11221 ctttatcaca ctgctatttt agtaccagat gaataccatc tcagtctagc tctaaatcat
    11281 ttattatctc agcatatccc tctagaaggt ggagctgacc atggctatag caatgctatc
    11341 tatctaagtg atcctgaagg taatggaatt gaaatttata atgataagga tatctctatg
    11401 tgggatatca gagagagtgg acaaattatt ggtattactg aaagactaga tattgacaat
    11461 ttacttgaca gccttgtcaa cgttcctaat aactataagc tttctgaaaa aactcgtatt
    11521 ggtcatattc atttgagtgt taaagatgct aagattagct caaaactcta tcaaaatgtt
    11581 tttggccttg atgagaaatt tgcaatcccg acagctagtt ggattgcatc cggtaactac
    11641 catcatcatc tagcctttaa taattgggct ggtccaaatc tttctaaaaa ccaagaaaat
    11701 agacccggaa tttccttatt gacaattgct tacaatgatg acaacctttt tagagatagc
    11761 ttaaaaaaag cgcaacttta ccaactgact ttccttgaaa aacaagatca ttattatatt
    11821 attgaagatt ttgatggcat ccgaattaaa gttgttctaa aaccagagaa ttaaccatca
    11881 cttgactttt gactcttcat tttacataat gtcaatatga taaaaatttt gatcccaact
    11941 gcaaaagaaa tgaaagtgtg tcaaaacatt gcaaggccta aactgagtgc acaaacgcaa
    12001 attataattg attatttttc tactttaact gtttcagact tagaggatat ctacagaatt
    12061 aatacaagtg cagctcgttg tgaggctcaa agatggcaag attttaaatg taaacaactt
    12121 actctgaatc ctgctattaa gttattcaac ggtcttatgt accgcaatat caaaaggcac
    12181 aacttatcca caagtgaagc acaatttatg gaaaattctg tttttattac tagtgctctt
    12241 tatggcatca ttcctgctat gacactaata tcccctcatc gtttagattt taacactaaa
    12301 atcaaaatca acaataactc tcttaaagtt ttttggcgtg aaaattacga tacttttatg
    12361 cagtcagatg acattatggt ttctttgctt tctaatgaat ttaaaaccgt cttttctcct
    12421 aaagagcgtc aaaaactaat acatcttaac tttattgaag atcgagatgg ccaattaaaa
    12481 acccactcta ccatatcaaa aaaagcaaga ggaaagtgtt taacttctat gatggaaaat
    12541 aattgccaaa ctctagaaca cttaaagcaa ttacgctttg atggcttctg ttatgataat
    12601 gaactctcag actctaagca attaactttt gttaaaaaac agacttaagt aactatttat
    12661 ccacaattta ttcacacaaa aagcaagaga taatcgtatc tcttgctttt tgacaaactt
    12721 gtaaggtcta aaaacctctc tatatcaagg tttcaaataa tattaacaaa aaataattgt
    12781 tatcaacaac ttgtggataa ctatggggat agacagagga taatctgttt atataaagtt
    12841 gattacttaa cagtaatgtc attgtatata cctttagatt tcaaaaatac ttgttaaatt
    12901 aacaataata aacgtcaaca tcttgtcaca gactgtcaaa tacttataaa caagctgtgg
    12961 ataaacataa ctttcaattt ctatttaaaa gttaattttt gacactaaag taaataatct
    13021 cgtttgactt gttcataact attgtcacca tcgtttaatc cttcccaaat cacgacttca
    13081 ccttctttta aagatttttg tacatcaatg attctttgat ttgaagatcc tctaaattgt
    13141 agcattaaat ttcgcttact ttgatcaaac ctaccatcca ctaaaatatc aatcaaagat
    13201 aacatctcca atttatcttg agtctccagc atcatttctt cccaagtata ccccgtccat
    13261 gaccaaatat ctttttctgg caattcacgt cggatacgct gaagaagtgg cagcaaaata
    13321 cctgtattta agaacggctc tccacctaat aacgttaaac cttggacgta aggttgtgcc
    13381 aaatctttca taatttgatc ttcaagctct tttgtataag ggattccggc tcgaaaggac
    13441 catgtagcag tattataaca ccctttacag tggaacatac atccagctac gtagagtgaa
    13501 tttctaacac cttcaccatc tacaaaatta aaagctttat aatcaatgat atggccatga
    13561 cttaattctt cgcttttcca ctccccaggc tttggagtat tccaacttgc ttcttctgac
    13621 atttctaacc tctctattga tatgaaaaga accagaacta actggtcttt ttattattat
    13681 cttaaagtac tatcttaggt tatccaataa cgctctgtct gatgacgaca atcttctaaa
    13741 ataccaccgt tagctaatat tactgacttg ctggcatcat tgtctacatg gcatgtcaca
    13801 agtatttcat tgatattttt tagatgtgct tgctcaattc ctagcttcaa ctgttcttta
    13861 gcataacctt taccacgctg gcgaggtcgt atggaataac caatatgtcc acctttttct
    13921 aaaagagtat catttagtct taaacgtaaa tttaagaaac ctatagcatt attgtcattg
    13981 tcaaaagaca caaactgaat atatggaaca aaattatcag gaacacctat ccctaactcc
    14041 atatttaggt tatgatctag ccagtcttca aaatgcgtag ttggttcata aaaaccacca
    14101 tccattgaac tactttcttt ttgaaactct ttatacatgg ctaaaatttc ttctttatct
    14161 tctaacactg ggcgccgtaa tttcatattg tttcctattc cattatcttc aagttaagca
    14221 ttccaaaagg agcattcgct ttattaatct ggtcacaaat aaaatgactc aataaactag
    14281 actttggtga tataggattg acacctgtag tgatagcttg aataaaagca tcaatcattg
    14341 gttcaaaacc acgtttctct agagtgcttg cccaagaacc aaaaccgata gcacgacggt
    14401 caaagccatc aataacagaa agatttgaca aatcatctaa tgaataggtt acttctggcg
    14461 actctatttc aattatttct cgacgagacc ccgactgcag gttcatgctt gcttctatta
    14521 tctcactgtc tgtttctagc gttactgata cttgtaatat cttattccca tcacgtttag
    14581 tcacaaagta tcctctttta acaacattat ttgtcaaaaa tagcgccgta tctaaaggat
    14641 ggataaacat atcaaaaagc ttatattgaa aatccgctgg tgcattgaca gcatttttaa
    14701 aggtgcgaat atggttttta tcttctactt ttttcatttc cataatacgt ggtgcaaagc
    14761 gacgattaaa acctgccatt aaaaatgttt tatgatcctt cgccaaatca tacaatgctt
    14821 tagttgaagt ataatcttct gtcaaaggct tatccataaa aactggaata ccctttttta
    14881 aaaaaagctt tgctatatca aagtgagcac tagtcgcgac atgaatgaag actccatcca
    14941 aaggatgctc ggctagacta tctaaactgt cataagctgt agaagaacca aaaagcatat
    15001 taacttcttc taaaatctgt ttttggcgtg taaaaagatg ccagtgcaca cctgtcactt
    15061 gacgcatata aggtagataa gctttctgtg aaatacctcc taaaccgaca attccaagtt
    15121 gcaacatagc atcctccttc tattttttct ttgccttttc caaaaattgt ttccttaaat
    15181 cttttagacg tgatttttga ttagcattct ctccagtaga gtgcttctca tggaacttct
    15241 gtacttgtgc ttgccctttg taatctaatt ggtatttccc catatcttaa taactccttc
    15301 tattttatct tcttatttta acatataata gaccagaggt tttaatagag gttttaatta
    15361 attaccttga tttttaatac ttgatccgtt catatgtttt acacgagcag agatttcctt
    15421 atgacgacca ttaaccatcg gacgagcctg tggattacct aagtaaccac atgtacgttt
    15481 gacaacatct actgtcttag ggtcgctgtt accacaattt ggacaagtaa agcctcgatc
    15541 tgtcggtgta aaatcccctt caaattgaca ttggtaacac ttatcaattg gtgtattagt
    15601 acctagataa cctacccgat cataagcata gtcccaaact gcttctaaag cttttggatt
    15661 ctgttgcaaa acagggtatt cacagtagtg aataaatcct cctgatgcac ccgtttcagg
    15721 atagattttc tcaaaatcta gtttttcaaa aggtgttgga ttcttacgca catcataatg
    15781 gaatgaatta gtgtagtatt ccttatcagt aatatcagtt actataccaa atttttcagt
    15841 atctaaacga caaaaccggt cagtcaagct ctccgatggt gtcgaataaa ctgaaaaatg
    15901 ataatcatac tcatcagacc aatctgcaca taactgcttc atcttacgaa cgatgtctac
    15961 agtaaatgct ttagcttgat gattatcttc ccagtcacca ccataaaaaa cactcgccac
    16021 ctcatataaa ccaatataac caagagacac cgttgcacgg cggtgattaa agagtcgatc
    16081 aacattctca tattttccta aacgttctcc aaatgctcca tactggtaaa gaatgggcgc
    16141 atttgctggg atagcctctt tgacacgttc cacacgataa accaaggcat ctctagcaat
    16201 actcatacgt tcattaaata tctcccagaa tttatccata tcaccgttag actccatggc
    16261 aacacgcggt agattaactg ttacaactcc aagattcata cgaccagatg ttacatcctg
    16321 accatttgca tctcgccacc cttgtaggaa ggaacgacag cccatagggg ctttaaaaga
    16381 gcccgttaag tcaataattt tatcataaga aaggacatct ggatacatcc gttttgtcgc
    16441 acactctaat gctaattgtt taatatcata attggggcta ttttcttcta aattaagacc
    16501 ttttttaagc gtaaaaatta attttggaaa aatagcagta cgatgctcgc ttcctaaacc
    16561 ttgaatccga attttcaaaa tcgctttttg aatttctcgt tcaaaccaat tagttcccaa
    16621 tccaaaacca agcgaagtaa atggcgtttg cccatttgaa gtaaataaag tattaatctc
    16681 atactccaaa gattgcatgg catcatatat atctttttgt gttttagcgc gcgcatagtc
    16741 ctcttgttta tcttcaataa cccattcttt tgcatctttt aaatgttttt gataatttaa
    16801 ctgtgcataa ggagctaaaa actcatcaat gcgatctgct gtacagccac catattgact
    16861 tgaagcaacg ttcgcaataa tctgtgaaat ctgagcagtc gctgtctgaa tagattttgg
    16921 actctcaact tctgcattac caatcttaaa accatttgca agcattccct taaaatcgat
    16981 taaacagcaa tttgtcatgg gagtgtatgg gctgtaatct aaatcatggt aatggatatc
    17041 acccttttga tgtgcatttg ctacatgaga aggtaacatt ttcagaccaa ttgatttccc
    17101 tacaatacct gctgttaaat cacgttgggt attaaacaca tcagagtctt tattagcatt
    17161 ctcattaaca acagtttgat ctttattaat taattttcca atagaaaaat tgatatctgt
    17221 agcctgtgaa cgctcaaaat cgcgttgggt acgataattg atatattctt gtgcaatagc
    17281 gtactcatta gcttccaata atttatgttc aacgatattt tgtatttcat atatcttgat
    17341 gttatcttta aaacgattgt agatctcagc gatgacatga tctgcaatca tctcaagttt
    17401 agcctcaact aaaggagaca ttttgataac tttattacta gcttttaata gagccgtgta
    17461 gattttatac ttatcaaagc tgaccaaacg cccgtcacgt ttaatcactt tgatatcaga
    17521 ttctgtcata aatcgttctc tttccaatac catcatgtga aatatcctcc agtgacaatt
    17581 atagcatgca aagaaaaaaa atcaatatat tgtgtcaagt tttttgctca gacacaatat
    17641 attgattttt taatctattt gaaacgatat agaacaaaat gagtgatatt tgatagagga
    17701 acttcttggt agtgcttctt aatatcagat ttcagttcat ttggtaactt ctcattttta
    17761 tttaagataa agtatttagc cccatgctga agcagctcgt ccttcaaaat tttctcatta
    17821 tttttttgcg ctgtattaat atgtggcaaa acaaattgcg aagcagattt taaccgagtc
    17881 tggctataaa tgctagcaac tttgtcccaa acataaatct taccatctct atcaattttt
    17941 gtagtcaaat aatgtgaaat gtcacgcctt tctttactaa gagtaacatt ttgataagta
    18001 ttataaacaa gtaaccctat caataaggaa actagtaaga ggggcaaata atatgttttc
    18061 tttacaaaaa ggtctacgat aagagattta ccagagaaat actgacgtct atgtgaacgc
    18121 ctgtcaatca ctttttggta aagaatagta attttattac tcgttagcaa aagaccaaat
    18181 ggtaaagcag gtaaaaagtg gtaaaggtta aaatctcttg aaaaaatggc cattatagcg
    18241 taaataataa aaatacaggc aataaagttc aagccgattt gataagatgc ttttttacga
    18301 ttttgaataa aatcccatag tccaaagagg caacctgacc ctagaaggac aacaatttga
    18361 attgctaaac taagaaggaa tgagtggttt gtcctagcga aatacgtaaa ggggtaaaaa
    18421 atagctttgt caatataaga agagataatc tgtagattaa acataaagta accaacagta
    18481 taagcaatta aaatcatacc aaaaacaacg cataaaaact gatagaaccc tcgtagtgac
    18541 tgcttttgct taatattggt tgctgttaag agaacaaaag agcaaagcca aaaaatcagt
    18601 gtttggggat ctataaatag tgagaatgct gctaaaatac cataaaatat aaatgcttca
    18661 tcaacgatat tatcgacaca aaatttaatt aaaaaccata gcgaaatgag cataaaaggt
    18721 aatgcccact gtattgggta cataccaccg aatcctaaga caatattcat tatatagaaa
    18781 atcaacgtca tgcctatcga gacaatttca ctttgtgcca cataataaac taatttataa
    18841 aaataaattc cagatacata gtaagcaata aactgtacaa taaataacca gatactagac
    18901 cctaaaaggt aacttagagc aatcgttaca tagtataaaa atcctcctgt agcaaaaaca
    18961 tcactataag ggatctgtcc tttggtcatc atcaaacctg tatagagatt ctgagtctgc
    19021 aaaccatttg caaaataagt caataaagga tttgcaattg ctaagagagt tgacaaaaca
    19081 ctatatagta aaattgaaaa tcgactagat ttcttcattc tgatagccgt tcgtttactc
    19141 attgatgggc tatttttata gctcactcca attggttttt ctaggttttc taccactatt
    19201 tttctcctca tatttacata ctaccagtat atcagaaaac cttatttagt caatttagcc
    19261 aacacttgac cgtaacgaat aatatcccta aagtagtggt atcttctaga catatattgt
    19321 ttgagaaatt cttgttcttc taatgttagc tgagtatctg acattaacat cacctccttt
    19381 tactacatca tcactattat tattcgaaaa aaaaaacgac tgaacaattg ctcaatcgct
    19441 tcaatattat tcttcatcta gaaagctgtt gaaaacttcc tcaatcatgt cccattctgc
    19501 atctgaatct tctggtatag gctgaaggtc tccttcagtt ccatcagcat tttctgtaaa
    19561 tgagtaagct tgaatctcaa tttcaccttg ttcgtcttct tctgcacctg caggaacgag
    19621 gagaacatag tttttgccaa actcttcacg tccatcaata gttagtagga tttcaaaaag
    19681 tgtttcgttc ccattctcat ctacaagagt gattacttca tgttgatgtt catggttatg
    19741 accgtggtta tgattatgtg ccattgtttc tcctttttat gccgaatgct taaaacatgc
    19801 ggtctaaata attttgtaag attagttgag ctgctaattt atcgataact ttcttacgct
    19861 taccacgact aatatcagct tgctcaacta gcatacgctc tgcttgaaca gtcgttagac
    19921 gttcatcttg atattcaact ggaagattga ataattcagt gattttatcg ccatatacct
    19981 gactagcctc tacacgagga ccacttgtat tattcatatt tttaggtaga ccaactacaa
    20041 atttatcaac tttgtactct tttactaact ctgctaagcg gtcaaaacca aaatttccag
    20101 attcttcatc gatcttaatg atttcaagac cttgtgcagt gaaaccgagt ggatcactaa
    20161 ttgcaacgcc aacagttttc gagccgacgt caagtcccat tattctcatt acaaatctat
    20221 tccatttcct tgtaaataat aacgaacgag ctcttctaca atctcatcac gttcatattt
    20281 acgaatttga ttgcgagcat cattgtaacg aggtacataa gcagggtccc cactcaatac
    20341 gtaaccaaca atttgattga tgggattgta acctttttct tctaatgaac gataaacagc
    20401 tgttaatgtt tcactaattt ctactttgtt actatcatct aatctaaaac gaacagtttc
    20461 atctgtaaat cccataccta caccttcttt cctttaattc tttcacttat tataacttaa
    20521 ataaagtatt tgtacaagaa attgataaac actatattag aaaatcaaaa agggagcaca
    20581 agaaaccaag aggtgattct ttgatttctt tactccctac cttataggtg atttttacag
    20641 gttatcatgt gactctcact tatgcttatc taaagctcaa agtgctgcac gaagacgagc
    20701 ttctgcattt tcaacgttac gaatagagcg aggtagaaaa gcacgaatat catcttcctt
    20761 ataacctact tgaagacgtt tgtcatcaat tagaattgga ctctttaaaa tacgtggatt
    20821 ttcttgaata agatcaataa cttcatttac actcaactcc tcaatattac aattgagcgc
    20881 cttagcataa cgatttttag aagaaacgat actttcaata ccactttcag tttttgttaa
    20941 tatttctaaa atttcatcac gtgttagtga ttctttacct aggttttgtt ctttatatgg
    21001 aagttgatga gcatttagcc aagtttttgc ttttttacaa cttgtgcaac ttgaaatagt
    21061 ataaatctt
//
LOCUS       DATRSG010000033        20349 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRSG010000033 DATRSG010000000
VERSION     DATRSG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20349
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.033"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(68..349)
                     /locus_tag="VBD89_001192"
     CDS             complement(68..349)
                     /locus_tag="VBD89_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135728.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
     gene            complement(383..769)
                     /locus_tag="VBD89_001193"
     CDS             complement(383..769)
                     /locus_tag="VBD89_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135729.1"
                     /translation="MVTILDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHFLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            complement(754..1434)
                     /locus_tag="VBD89_001194"
     CDS             complement(754..1434)
                     /locus_tag="VBD89_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135730.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            complement(1380..1967)
                     /locus_tag="VBD89_001195"
     CDS             complement(1380..1967)
                     /locus_tag="VBD89_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135731.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            2376..2690
                     /locus_tag="VBD89_001196"
     CDS             2376..2690
                     /locus_tag="VBD89_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7135732.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRIIAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2706..3092
                     /locus_tag="VBD89_001197"
     CDS             2706..3092
                     /locus_tag="VBD89_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7135733.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            3121..4506
                     /locus_tag="VBD89_001198"
     CDS             3121..4506
                     /locus_tag="VBD89_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7135734.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            4509..4661
                     /locus_tag="VBD89_001199"
     CDS             4509..4661
                     /locus_tag="VBD89_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7135735.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4684..5889
                     /locus_tag="VBD89_001200"
     CDS             4684..5889
                     /locus_tag="VBD89_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7135736.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            5932..6153
                     /locus_tag="VBD89_001201"
     CDS             5932..6153
                     /locus_tag="VBD89_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135737.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            6270..6767
                     /locus_tag="VBD89_001202"
     CDS             6270..6767
                     /locus_tag="VBD89_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO7135738.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            6742..7248
                     /locus_tag="VBD89_001203"
     CDS             6742..7248
                     /locus_tag="VBD89_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7135739.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            7232..9679
                     /locus_tag="VBD89_001204"
     CDS             7232..9679
                     /locus_tag="VBD89_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7135740.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            9682..11859
                     /locus_tag="VBD89_001205"
     CDS             9682..11859
                     /locus_tag="VBD89_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7135741.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSITT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            11856..12857
                     /locus_tag="VBD89_001206"
     CDS             11856..12857
                     /locus_tag="VBD89_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO7135742.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            12854..13789
                     /locus_tag="VBD89_001207"
     CDS             12854..13789
                     /locus_tag="VBD89_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7135743.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            14034..14150
                     /locus_tag="VBD89_001208"
     CDS             14034..14150
                     /locus_tag="VBD89_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7135744.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14166..16085
                     /gene="tet(M)"
                     /locus_tag="VBD89_001209"
     CDS             14166..16085
                     /gene="tet(M)"
                     /locus_tag="VBD89_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7135745.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTSFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            16204..16371
                     /locus_tag="VBD89_001210"
     CDS             16204..16371
                     /locus_tag="VBD89_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7135746.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(16431..16784)
                     /locus_tag="VBD89_001211"
     CDS             complement(16431..16784)
                     /locus_tag="VBD89_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7135747.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            17289..17711
                     /locus_tag="VBD89_001212"
     CDS             17289..17711
                     /locus_tag="VBD89_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7135748.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            17708..17938
                     /locus_tag="VBD89_001213"
     CDS             17708..17938
                     /locus_tag="VBD89_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7135749.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            18399..18602
                     /locus_tag="VBD89_001214"
     CDS             18399..18602
                     /locus_tag="VBD89_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7135750.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            18684..19901
                     /locus_tag="VBD89_001215"
     CDS             18684..19901
                     /locus_tag="VBD89_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7135751.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(20023..>20349)
                     /locus_tag="VBD89_001216"
     CDS             complement(20023..>20349)
                     /locus_tag="VBD89_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135752.1"
                     /translation="FYMKEQQRKEELKRNREYEVSLVKALKNSYSDIKEIQISNPITT
                     TMPGDWRSTVKIIFSDNDEITYCITHNKDDEINRSGSINEKEHLFLSKTKYKFLIIFL
                     YFLKCS"
BASE COUNT         6541 a         3672 c         4055 g         6081 t
ORIGIN      
        1 caacttgatt attttcatat ctataataac tatcaggata gtaagcttcc tcaacatttt
       61 cactcgatta taaatcatca taactacctt tatttaaatc tacctgtgcc tttacttttt
      121 cccaaaattg tcctacaagt ccagtgtcaa tttggttttt tatcgttttt attgatgatt
      181 tagcagtctc agaaatatca tcaatctttt cactataagt cgtcttaata gaatcagctt
      241 cttctactat ttccggggca tggacaaacc ttactaaagc tttttgaatt tgctttttga
      301 attctttgtc ttctttggtt cctttcaata cagcgcctat taaactcatt tattgcctct
      361 ttcactttat agtccatcta aattagtagt attttcttcc ttctaaacta gaaatgtcat
      421 tttctaattt tactcggtct tttagaaact gtgtccgact attttcctct tggacaatga
      481 tagtctgttt atagttttca tattcatcaa ttaattccat ttgataactt tcaattattc
      541 gatttaaact cataaaagtc gaaccatctg aatcattttg tctcaaaaag tgacttgtct
      601 cttcatagac ttcttgtaaa taagtctcta actcgttctt tttatcctca aggctatata
      661 atttatcttc tatctccata ttttcgcgat gataattatc ttccaatttc cctaggttta
      721 tttttagact attaattttg ttttcatttt gatctagtat tgtgaccatt ccttaaattc
      781 tcctgctaat ttggagtcct cttctagcaa tttttggaca ccagattcaa tttgtttttg
      841 aagattagta tatgaagttg tgatctcttg tgcattagta actttttcat caaagaaagt
      901 ttcaattggt gttactatgg tgtcctctct aactccaccc gcactatatg catcagctaa
      961 ttcttctgga cttaaatctg ttataccagg aggcatcact ttagtttttg caaatagttc
     1021 tgatgctttt tctttagcta attcagcatt cttcttgata tcatcagaag ctgtttgaga
     1081 tgccgtggtt aaaccgttag caataaatat agcctgttca gaatctaaat atactttttc
     1141 actatgacta agtttgcctc cagatttttt taatttagct ttagcttttt gaaaatgaac
     1201 ttcttcataa agaacagact gaacagctgg tgacttagga tttacgatgt tacctttttc
     1261 atcttttaac tgaccatcat cagtaaattt ccaactctct attttatgac ttttaccatt
     1321 tacccatttc acggtaccat aacctttatc atctagttta ttaaaatcgt tccaccaagt
     1381 tacattatcc tctttatgtc gaaaattagc aaaatattca ggatgcttag ccatgaattt
     1441 ttttcatctt catttaaagt gctataccta aaccatccat taaatactgt agtcgggata
     1501 tgatatttag cacctaattt tagcatataa gcagattgag aatagcctga aagctgagtc
     1561 acagtccatt tgtcatgtga ctgtacgtct tttaaaaatt tatcagctga ttttaactga
     1621 gtcgaacttt gatgtggttg agcagatgaa actgcgccaa ttaaatcttt ataattactt
     1681 gaatccgttg cggcggaaat aactgcaaca ctgttataat ctactttacc cttgataatt
     1741 ggtgcaactg ccataccctg atagccagac atatcgtcag gagaagtttt taatacctta
     1801 aacgctttac ccgtctttgt tactattaaa tttgtttcat tagatttttt tggattagct
     1861 gttattattt ttccgccatt ttgtttcaca tagtttggat ttacactata tacgttctca
     1921 ccttgattca atctatttaa atcatactcg ctataagaat atgtcattat cttttttcct
     1981 tttctcctgt agaaaactca actaaaatat gattttttgt tcttccatat ttttaattta
     2041 aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg
     2101 tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt
     2161 tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta
     2221 gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat
     2281 gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc
     2341 attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca
     2401 acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa
     2461 gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa
     2521 gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg
     2581 aggaacgtgt gaagttagtc aatccacgta ttatcgcaga gggctacaaa atcggcactc
     2641 gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag
     2701 gaaaaatgat gagattagca aatggcattg tattagataa agacacgact tttggagaat
     2761 tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag
     2821 atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag
     2881 taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta
     2941 tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt
     3001 atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac
     3061 aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc
     3121 atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg
     3181 atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc
     3241 cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga cattccctat
     3301 ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa
     3361 cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata
     3421 cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt
     3481 gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat
     3541 ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac
     3601 ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat
     3661 tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa
     3721 gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag
     3781 ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca
     3841 ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct
     3901 gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt
     3961 tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg
     4021 aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc
     4081 tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg
     4141 aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct
     4201 tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatcagtt taatttcaga
     4261 gtggctttag gtcgtatgtc tgaaatgggc tatggcatga tgtttggcag tgacgtacaa
     4321 aaggatttct tcttaaagcg aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc
     4381 atatcagagt tttatactcc ccttgtacca aaaggatatg atttcttgga ggaaattaaa
     4441 aagttatcca acagcagaca gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg
     4501 gactgatctt gctggctggt gtggcaatag ccacgccagc acttaacccc ccgtatctaa
     4561 caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca
     4621 agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca tacgcctttt
     4681 tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa agaaaaacgc
     4741 ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac aaggcagtat
     4801 ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca gtccctttgg
     4861 gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga ttatgtaagg
     4921 attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca actgaaactg
     4981 tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta tgctttaggc
     5041 gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga attgaaaggt
     5101 cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg gtatgagttc
     5161 tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat
     5221 aagacaggga ttttgaatat ccctgtactc actgaaaagt gccaacagga agaatgtatc
     5281 tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa agaggaaaag
     5341 gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta tttctgtatc
     5401 tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga cgcagaagta
     5461 aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt ccgtgattta
     5521 ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta tatccgtttt
     5581 gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga atgggcttgg
     5641 tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc ttactccttc
     5701 caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt tgcgattaaa
     5761 cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc gaaactgaca
     5821 gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt gataacaaca
     5881 aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt tatgaatttt
     5941 ggacaaaacc tttataactg gtttctatca aacgctcaat cactggtgct tttagcaatc
     6001 gttgtgattg gcttgtatct tggcttcaag cgtgagttta gcaaactgat tggcttttta
     6061 attattgcga ttattgcggt tggcttagtc ttcaacgctg ctggagtaaa agacatttta
     6121 ctagagctat tcaatcgcat tattggtgct taaataaaac cgttcttttg tggaatataa
     6181 gtggttttct tatgttccgc aaaggaatgg tacaccaaac gaagtgcggt agggattttt
     6241 gaatctctac aaagaaagga cgtgaatata tggacgatat gcaagtctat attgcgaatt
     6301 taggcaaata caatgagggc gaattggtcg gtgcgtggtt tacctttccc attgactttg
     6361 aggaagtcaa agagaaaatc ggcttgaatg atgaatatga ggaatacgcc attcatgact
     6421 acgagttacc ctttacggtt gacgaataca cttccattgg cgaactcaat cgactatggg
     6481 aaatggtatc ggaattaccc gaagaattac aatcggagct atctgctctg ctcactcatt
     6541 tttcaagcat tgaagaacta agcgaacatc aagaggatat tatcattcat tccgattgtg
     6601 atgatatgta tgacgtggca cgctactaca ttgaagaaac gggtgcttta ggcgaagtac
     6661 cagctagtct tcaaaactat attgattatc aagcctatgg tcgggattta gacctttcag
     6721 gaacgtttat ctcaaccaat catgggattt ttgaaatcgt ctattaaatc tgtcggtaca
     6781 ttactactgg cagattttct attttacggg gtggctcaat cagctacccc tattttttat
     6841 gaaaggattg attacatgaa gaaaatacga agctatacca gtatctggtc tgtggaaaag
     6901 gtactgtatt ctatcaatga ttttagactt ccgtttccca taacctttac gcaaatgaca
     6961 tggtttgtcg tgtcactctt tgcagtgatg atacttggca acttgccccc tctttccatg
     7021 atagagggag catttctcaa atactttggg attcctgtgg ctttcacatg gtttatgtct
     7081 acaaaaactt ttgatggtaa aaagccttat ggatttttga agtctgtcat tgcttatgca
     7141 ctgcgaccaa agctgaccta tgcaggaaaa aaagtaacgc ttggcagaaa ccagccacaa
     7201 gaagccatta cagcagttag gagtgaattt tatggcatat ccaattaaat acattgaaaa
     7261 caatctcgtc tggaataaag acggggaatg ttatgcttac tatgagcttg ttccttacaa
     7321 ttactcattt ctaagtccag aacagaaaat acaagtgcat gattctttca gacagcttat
     7381 cgcacaaaat cgtgatggca aaattcatgc tttacaaatc agtacagaat ccagcatacg
     7441 ttctgcacaa gagcgttcca aaaatgaagt cactggcaag ctcaaagcgg ttgcctatga
     7501 caaaatcgac caacagacag acgctttaat atccatgatt ggcgaaaatc aagtgaacta
     7561 ccgtttcttt atcggcttta agttgcttct caacgatcag gagttttcta tgaaaagtct
     7621 taccgttgaa gcaaaaaatg ctttgtctga ttttgtctat gatgtgaacc ataagctgat
     7681 gggcgatttt gttagtatga gtaatgatga aatcctgcgt tttcagaaga tggaaaagct
     7741 cttagaaaat aaaatctctc gtcgtttcaa aatccgcagg ttagataagg acgacttcgg
     7801 ctatctgatt gaacaccttt acggacagac aggcactgcc tatgaagagt atgagtacca
     7861 tctatcaaag aaaaagctgg ataatgaaac gctgattaaa tactatgact tgattaagcc
     7921 tactcgctgt ttggtggaag aaaaacagcg atatttgaaa atccagcagg aagatgaaac
     7981 cgtctatgta gcttacttta ccattaacag cattgtcgga gaactggact tcccgtcctc
     8041 tgaaatcttc tactaccagc aacagcaatt tacattcccg attgatacgt caatgaatgt
     8101 ggaaattgta gcgaatcgta aagccctatc tactgtccgc aataaaaaga aagaactgaa
     8161 agacttggat aaccacgctt ggcaaagtga taatgaaacc agctccaatg tggcggaagc
     8221 tctggaaagt gtgaatgagc tggaaaccaa tttagaccaa agcaaggaat ctatgtacaa
     8281 gctgtcttat gtggtaaggg tatcagcaaa tgatcttgac gaactcaaac gtcgttgtaa
     8341 tgaagtgaaa gatttttatg acgatttaag cgtaaaactg gtacgaccat ttggggatat
     8401 gctcggctta catgaagaat ttttacctgc cagcaagcgt tatatgaatg attatattca
     8461 atacgtgacc tctgatttcc tcgctggttt aggttttggt gctactcaaa tgctggggga
     8521 aaatgagggg atttatgttg gctacagctt agatactgga cgcaatgtct atctgaaacc
     8581 tgctcttgcc agtcaagggg ttaagggttc agtaaccaat gcgttagcgt cggcttttgt
     8641 tggttcgctg ggtggtggta aatcctttgc gaataacctt atcgtctatt atgcggtgct
     8701 ttatggggca caagcagtga ttgtagaccc aaaagcagaa cgtggcagat ggaaagaaac
     8761 cttgccagag atttcccatg aaatcaatat cgtcactctg acttctgatg agaaaaacaa
     8821 aggcttactt gacccttatg tgattatgaa aaatcccaaa gattctgaat cactggctat
     8881 tgatattctg acattcctta cggggatttc ctctcgtgat ggggaacgct tcccaatcct
     8941 tagaaaagcc attcgtgcag taaccaatag tgaagtacga gggttgatga aagtgattga
     9001 ggaattacgg gttgagaata cgccactaag taccagtata gccgaccata tcgaaagttt
     9061 tacagactat gactttgcac atttattatt cagtaatggt tatgtggagc agtctatcag
     9121 cttagaaaaa caactgaaca ttatacaggt tgcggacttg gtacttcccg acaaggaaac
     9181 ttcctttgag gaatatacca ctatggagct tttatccgtt gctatgctga ttgtcattag
     9241 tacctttgct ttagacttta tccatacaga ccgaagcatt ttcaagattg tagatttaga
     9301 cgaagcatgg agctttttac aggtagcaca aggaaaaaca ctatctatga agctggttcg
     9361 ggctggtcgt gctatgaacg ctggggtata tttcgtgacc caaaatacag acgacctctt
     9421 agatgaaaaa ctgaaaaata acctcggctt aaaatttgca tttcgttcca ctgaccttaa
     9481 cgagattaaa aagaccttag ccttttttgg tgtagaccca gaggacgaaa acaatcagaa
     9541 gcgattgcgt gatttggaaa acgggcaatg ccttatcagt gatttatatg gtcgtgtcgg
     9601 tgtgatacag ttccaccctg tatttgaaga actgctccat gcctttgata ccagaccacc
     9661 tgtgcgaaaa gaggtgtaaa tgtgaaacca tcaatagtaa acagaataaa atcaaactgg
     9721 acgctgaaac gtctaggtaa agtggcaatg acagtggctt tcacacttgt gattgccatt
     9781 tttcttttag ccatgctggg aacggtggtt caagctgcgg gcttggtaga tgatacggtc
     9841 aatgtggcaa atgaatacag ccgataccca cttgaaaact atcaactgga tttttatgtg
     9901 gataatagct ggggctggct tccgtggaac tggtcggacg ggattggaaa acaggtcatg
     9961 tatggactat atgccattac caattttatt tggacaatca gtttgtatgt ttccaatgcg
    10021 acaggttact tagtacagga agcctattcc ttagacttca tttccgctac agcagattcc
    10081 attggtaaga atatgcagac cttagctggt gtgagtgcaa acggattttc aacagagggt
    10141 ttctatgttg gattcctctt actcttgatt ttggttcttg gggtttatgt tgcctatacg
    10201 ggactgataa agagagaaac cacaaaggca attcatgcca ttatgaattt tgtgctggtg
    10261 tttatcctat cggcttcctt tattgcctac gctcccgact acattaaaaa aatcaatgac
    10321 ttttcatcag acatcagtaa tgccagttta tcacttggca cgaagattgt catgccccat
    10381 tccgatagtc aaggcaagga cagcgtggac ttaatcagag atagcctgtt ttccatacag
    10441 gttcagcaac cgtggctact gcttcaatac aacagttcag acattgaaag tatcggtatt
    10501 gaccgtgtgg aaagcctgct ctccaccagc ccagattcca acaatggcga agacagagaa
    10561 aaaattgttg cggaagaaat tgaagacaga agcaatacca atctaaccat tacaaagacc
    10621 attaaccgtt taggtacagt cttcttccta tttgtcttca atattgggat ttccatattt
    10681 gtattcctat taacaggaat catgattttc tcgcaggtac tttttatcat ctatgctatg
    10741 tttctgcctg tgagctttat tttaagcatg attccatcat ttgatggtat gtcaaaacga
    10801 gccataacaa agctctttaa taccattttg acacgagctg gaatcacatt gattattacg
    10861 acagcattta gtatttcaac catgctctat accttatcgg ctggttatcc gttctttttg
    10921 attgcttttc tacagattgt gacctttgca ggaatctact tcaagctggg cgatttaatg
    10981 agtatgtttt ctctacagag taacgattct caaagtgtgg gaagtcgtgt gatgagaaaa
    11041 cctcgtatgc ttatgcacgc tcacatgcac cgtctacagc ggaaacttgg acgttccatg
    11101 actactctag gggctgggtc tgccattgtt acaggtaaaa aaggacagtc gggttcgggg
    11161 agttctgcaa ggacacaagc agatcactcc cgaccagacg gaaaggaaaa atcaacactt
    11221 ggaaaacgta tcggtcaaac catcggtaca gtagctgata ccaaagacag aatggtagac
    11281 actgctagtg gtttgaaaga acaggttaaa gatttgccga ccaatgcaag atatgcagta
    11341 tatcaaggaa aatccaaagt aaaagagaat gtccgtgatt taaccagtag tatttctcaa
    11401 accaaagcgg acagagccag tggacgcaag gaacagcagg aacaaaggcg aaaaaccatt
    11461 gcgaagcgtc gctctgaaat ggaacaggtc aaacagaaaa aacagcctgc ttcttctgtt
    11521 catgaaagac cgactacaag acaagaacaa tatcatgatg aacagacctc aaaacagtct
    11581 aatattcaga cttcatataa ggaatctcaa caagccaaac aagagcgtcc agcagttaag
    11641 tccgattttt caagtccaaa agtggaacgc caaggcaata ccgttcaaga aaaaaccgtt
    11701 caaaagccag caacttcaat cactacagca gatagaactt cacaacgtcc aatcacaaaa
    11761 gaacgtccgt ctactgttca aagagtacca ctacaaaata caagaagtag accaccaatc
    11821 aaaaccgcca ccattaagaa agtcggtaag aaaccatgaa gttgaaaact ttagtgattg
    11881 gtggttctgg attattcttg atggtcttct cactgcttct gtttgttgcc attttatttt
    11941 cagatgaaca ggacagcgga atttccaata ttcattatgg aggtgtgaat gtttccgcag
    12001 aagtgctggc tcataagcct atggtagaaa aatatgccaa agaatatggc gttgaagaat
    12061 atgtcaacat acttcttgcg attatacagg tggaatcggg cggtactgcg gaagatgtta
    12121 tgcagtcctc ggaatccctc ggtcttccac ctaattcatt gagtacagaa gaatccatta
    12181 agcaaggtgt gaagtatttc agtgaattat tagccagtag cgaaaggctc agtgtagatt
    12241 tagaatcggt tatccagtcc tacaattatg gtggtggttt cttagggtat gtggctaatc
    12301 gtggaaataa atataccttt gaactggctc aaagtttctc aaaagagtat tcaggtggcg
    12361 aaaaagtgtc ttaccccaat cccatagcca tacctatcaa tgggggctgg cgatacaact
    12421 atggcaatat gttttatgtg caactggtaa cgcagtatct tgtcacaaca gagtttgatg
    12481 atgatacggt acaagccatc atggacgaag cactgaaata tgagggctgg cgatacgttt
    12541 acggtggagc ttccccgact acttcttttg attgtagcgg actgacacaa tggacgtatg
    12601 gaaaagctgg aattaactta ccacgaaccg cacaacagca atatgatgtg acccagcata
    12661 tcccactatc ggaagcacaa gctggcgatt tggttttctt tcattctacc tataacgctg
    12721 gctcttatat tactcatgtt gggatatacc ttggcaataa ccgtatgttt catgcaggcg
    12781 acccaatcgg ttatgccgac ttaacaagcc cctactggca acagcattta gtgggagcag
    12841 gacgaatcaa acaatgagaa aggaagattt aatgatgaaa tttagaaaaa atcagaataa
    12901 agaaaaacag ataccaaagg aaaagaaacc tcgtgtctac tataaggtca atcctcataa
    12961 aaaggttgtg attgccttgt gggtactttt agggcttagt ttcagctttg cgatattcaa
    13021 gcactttaca gctatagata ctcatactat tcacgaaaca actatcatag aaaaggaata
    13081 cgttgatact catcatgtag aaaattttgt agagaacttt gcgaaagtct actattcatg
    13141 ggagcaatcc gataagtcca ttgataatcg aatggaaagt ctaaaaggct atctgacaga
    13201 tgaacttcaa gctctcaatg ttgatacagt acgcaaagat attcctgtat cgtcttctgt
    13261 aagaggattt cagatatgga cggtagagcc aactggcgac aatgagttta atgtaaccta
    13321 cagtgtagac cagctcatta cagagggaga aaatacaaag accgtccact ctgcttatat
    13381 agtgagtgtc tatgtagatg gttctggaaa tatggtactg gttaagaatc cgaccattac
    13441 caacatacct aagaaatcaa gttataaacc aaaagccatt gaaagtgagg ggacggttga
    13501 ttccattaca accaatgaaa tcaatgagtt tttaacgacg ttcttcaagc tctatcctac
    13561 agcgacagcc agtgaacttt cctactatgt gaatgacggg atattaaaac caatcggaaa
    13621 agagtacatc tttcaagaac tggtaaatcc tattcacaat cgtaaggata atcaagtcac
    13681 ggtatcgctg acagtggagt atatcgacca gcagaccaaa gcaacgcagg tatctcaatt
    13741 tgatttggta cttgaaaaga acgggagtaa ttggaagatt atagaataac aaatattggt
    13801 acattattac agctattttg taatcacgta ctctctttga taaaaaattg gagattcctt
    13861 tacaaatatg ctcttacgtg ctattattta agtatctatt taaaaggagt taataaatat
    13921 gcggcaaggt attcttaaat aaactgtcaa tttgatagtg ggaacaaata attggatgtc
    13981 cttttttagg agggcttagt tttttgtacc cagtttaaga atacctttat catgtgattc
    14041 taaagtatcc ggagaatatc tgtatgcttt gtatgcctat ggttatgcat aaaaatccca
    14101 gtgataagag tatttatcac tgggattttt atgccctttt gggcttttga atggaggaaa
    14161 atcacatgaa aattattaat attggagttt tagctcatgt tgatgcggga aaaactacct
    14221 taacagaaag cttattatat aacagtggag cgattacaga attaggaagc gtggacagag
    14281 gtacaacgaa aacggataat acgcttttag aacgtcagag aggaattaca attcagacgg
    14341 cgataacctc ttttcagtgg aaaaatacta aggtgaacat catagacacg ccaggacata
    14401 tggatttttt agcagaagta tatcgttcat tatcagtatt agatggggca attctactga
    14461 tttctgcaaa agatggcgta caagcacaaa ctcgtatatt gtttcatgca cttaggaaaa
    14521 taggtattcc cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa
    14581 cggtttatca ggatattaaa gagaaacttt ctgcggaaat tgtaatcaaa cagaaggtag
    14641 aactgcatcc taatatgcgt gtaatgaact ttaccgaatc tgaacaatgg gatatggtaa
    14701 tagaaggaaa tgattacctt ttggagaaat atacgtctgg gaaattattg gaagcattag
    14761 aactcgaaca agaggaaagc ataagatttc ataattgttc cctgttccct gtttatcacg
    14821 gaagtgcaaa aaacaatata gggattgata accttataga agtgattacg aataaatttt
    14881 attcatcaac acatcgaggt cagtctgaac tttgcggaaa agttttcaaa attgagtatt
    14941 cggaaaaaag acagcgtctt gcatatatac gtctttatag tggcgtactg catttgcgag
    15001 attcggttag aatatcggaa aaggaaaaaa taaaaattac agaaatgtat acttcaataa
    15061 atggtgaatt atgtaaaatc gataaggctt attccgggga aattgttatt ttgcagaatg
    15121 agtttttgaa gttaaatagt gttcttggag atacaaagct attgccacag agagagagaa
    15181 ttgaaaatcc cctccctctg ctgcaaacga ctgttgaacc gagcaaacct caacaaaggg
    15241 aaatgttact tgatgcactt ttagaaatct ccgacagtga cccgcttctg cgatattatg
    15301 tggattctgc gacacatgaa atcatacttt ctttcttagg gaaagtacaa atggaagtga
    15361 cttgtgctct gctgcaagaa aagtatcatg tggagataga aataaaagag cctacagtca
    15421 tttatatgga aagaccgtta aaaaaagcag agtataccat tcacatcgaa gttccaccga
    15481 atcctttctg ggcttccatt ggtctatctg tagcacagct tccattaggg agcggagtac
    15541 agtatgagag ctcggtttct cttggatact taaatcaatc gtttcaaaat gcagttatgg
    15601 aggggatacg ctatggctgt gaacaaggat tgtatggttg gaatgtgacg gactgtaaaa
    15661 tctgttttaa gtatggctta tactatagcc ctgttagtac cccagcagat tttcggatgc
    15721 ttgctcctat tgtattggaa caagtcttaa aaaaagctgg aacagaattg ttagagccat
    15781 atcttagttt taaaatttat gcgccacagg aatatctttc acgagcatac aacgatgctc
    15841 ctaaatattg tgcgaacatc gtagacactc aattgaaaaa taatgaggtc attcttagtg
    15901 gagaaatccc tgctcggtgt attcaagaat atcgtagtga tttaacttcc tttacaaatg
    15961 gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt tactaccggt gaacctgttt
    16021 gccagccccg tcgtccaaat agtcggatag ataaagtacg atatatgttc aataaaataa
    16081 cttagtgtat tttatgttgt tatataaata tggtttcttg ttaaataaga tgaaatattt
    16141 tttaataaag atttgaatta aagtgtaaag gaggagatag ttattataaa ctacaagtgg
    16201 atattgtgtc ctgtatgtgg aaataaaaca cgattaaaga taagggaaga tactgaatta
    16261 aaaaaattcc ccctctattg tccgaaatgc agacaagaaa atttaattga aataaagcag
    16321 ttcaaagtaa ctgtgattac agagccagac gcaaagacgc agagccgata aaatgagatt
    16381 aatacaatct cattttatcg gctctttccg ttatgtatgg attcttttaa ttagtcttcg
    16441 atgtttcttg cttcgttgat accgctggct aaagattcca ttaaggatag ttctttgtct
    16501 gtaaagctat ccatgtattt ctctatctgt aatcgtcggg tgctttttac caagttatta
    16561 gcaggtaaga aaaattcatc aacggaaaca tgaagtaacg atacaaggtc ataaagaact
    16621 tgtatgctgg ggtgttgccc tttattttca atattagtta agtaccgtgg gtcaatttca
    16681 atcaatgctc ccacttgttc acgagttaaa cctcgtttca atcgagcttc tttaatggct
    16741 aaaccaaagg ctctaaaatc atatttatct tcttttttac gcatagtaga ccacctctat
    16801 acattttatt gttcctactg aattaaaaac aggtatagaa aaacgtgtta tatggtttat
    16861 aggtttatat ttaataaaaa gcactactaa acgccaataa aaaaaaccgt tatatggtag
    16921 tgctatttac gctgttaaaa tattgtatat tacttccaaa tggcggtttg ttggaggtca
    16981 acgtcgccat gaagtacatc atatacaata aatttcctta cattgggttc ttgtcaaaaa
    17041 aagtcgtcta tctgcaatag ataagtacgt ccaccaatgt ggttttataa atcatataga
    17101 tagaataaca gaagcatgta aacagagaaa taaatctgtt tatatgcttt tttggctatt
    17161 cagaactttt ttacaaagtt tatttatcag taatgcaaca aatccccctt tcacattggg
    17221 actaagagtg aaaggagata aacgagcaag gctcacttcc tttcctagac agaaaggggg
    17281 tgagaaacat gaaaccatct tcttttcaga ccacaataga aaatcagttt gactatatct
    17341 gtaaacgtgc tatggaagac gagcgaaaga attatatgct ttatctttca aggattgcaa
    17401 agcgtgaggt gtccttttcg gatgttggcg attatcttgt tagccagttt gcgacaacag
    17461 ataactattc aactgacttt cagattttta cactcaatgg gttatcagta ggcgttgaaa
    17521 atgatttgtt gagtgaagca ttacgtgagt tgccagacaa gaaacgtgaa attctactgc
    17581 tgttttactt tatggacatg agcgattcag aaattgcaga cctgttgaaa ttgaaccgtt
    17641 ctactgtcta tcggcataga accagtggac tagccttaat taaaaagttt atggaggaat
    17701 ttgaagaatg aaaacacaat atcctatgat tccctttcct ctcattgtaa aggcaacaga
    17761 tggcgatacc gaagcgatta accagattct acatcattac agagggtaca taacgaagcg
    17821 ttccctacga cttatgaaag atgaatatgg caatcaaagt atggtcgttg atgaagtctt
    17881 acgtggaaga atggaaacca gactgattac aaagattttg tcatttgaaa ttaagtaata
    17941 tcctctctcc tttcgtggaa gcgtgctaaa ccattccacg cttcccgaac agggaggttt
    18001 gttattccac caaagcatat tgagctttca atgtgttttg ataggctaac gagccattgt
    18061 tctttgaaaa ctgaataaaa gtaatcgaat acgtttcgat aagaaaagag ccaacggaac
    18121 taaccgccat gacctatctt ataaagatag cgagcgattc atgttagtga tccgagaagc
    18181 aatctttagc aggattgcct gcaacgacat tcttatcgtg ataatgatac tcccatacag
    18241 tcaatagtcc gagcgtgata aaaccgtcgc aggcaatgag tatggctaca tgagaaccat
    18301 gcaggggtgg aactcccgtg agctttgcta aagctgttcg attgctggta aaacaacttt
    18361 tatgaaatac aaataagtga tttggaaagg aggattttat gaagcagact gacattccta
    18421 tttgggaacg ttatacccta accattgaag aagcgtcaaa atattttcgt attggcgaaa
    18481 acaagctacg acgcttggca gaggaaaata aaaatgcaaa ttggctgatt atgaatggca
    18541 atcgtattca gattaaacga aaacaatttg aaaaaattat agatacattg gacgcaatct
    18601 agcgtcgcca aagggtcttg tatatgataa aatagtatta agtcgtatca aggctctttc
    18661 cataaaggaa aggagcaaat gccatgtcag aaaaaagacg tgacaataaa ggtcgaatct
    18721 taaagactgg agagagccaa cgaaaagacg gaagatactt atacaaatat atagattcat
    18781 ttggagaacc gcaatttgtt tactcgtgga aacttgtggc tacagaccga gtaccagcag
    18841 gaaagcgtga ttgtatctca cttagagaga aaatcgcaga gttacagaaa gacattcatg
    18901 atggtattga tgttgtagga aagaaaatga cactctgcca gctttacgca aaacagaacg
    18961 ctcaaagacc aaaggttaga aaaaacactg aaactggacg caaatatctt atggatattt
    19021 tgaagaaaga caagttaggt gtaagaagta ttgacagtat taagccatca gacgctaaag
    19081 aatgggctat tagaatgagt gaaaatggtt atgcttatca aaccatcaat aactacaaac
    19141 gttctttaaa ggcttcattc tatattgcta tacaagatga ttgtgttcgg aagaatccat
    19201 ttgactttca actgaaagca gttcttgatg atgatactgt ccctaagacc gtactaacag
    19261 aagaacagga agaaaaactg ttagcctttg caaaagctga taaaacctac agcaaaaatt
    19321 atgatgaaat tctgatactc ttaaaaacag gtcttcgtat ttcagagttt ggtggtttga
    19381 cacttccaga tttagatttt gagaatcgtc ttgtcaatat agaccatcag ctattgagag
    19441 atactgaaat tgggtactac attgaaacac caaagaccaa aagtggcgaa cgtcaagttc
    19501 ctatggttga agaagcctat caagcattta agcgagtgtt agcgaatcga aagaatgata
    19561 agcgtgttga gattgatgga tatagtgatt tcctctttct taatagaaag aactatccaa
    19621 aagtggcaag tgattacaac ggcatgatga aaggtcttgt taagaaatac aataagtata
    19681 acgaggataa attgccacac atcactccac atagtttgcg acatacattc tgtaccaact
    19741 atgcaaatgc aggaatgaat ccaaaggcat tacagtacat tatgggacat gctaatatag
    19801 ccatgacgct gaactattac gcacatgcaa cattcgattc tgcaatggca gaaatgaaac
    19861 gcttgaataa agagaagcaa caggagcgtc ttgttgctta gtagtacaaa tgaatttact
    19921 acttatttac cacttctgac agctaagaca tgaggaaata ttcaaagaaa cgtgaagtat
    19981 cttcctacag taaaaatact cgaaagcaca tagaataagg ctttacgagc atttaagaaa
    20041 atataaaaag ataattagaa atttatactt tgttttactt aaaaataaat gttctttttc
    20101 atttattgat ccactgcgat taatttcatc atctttatta tgggttatac agtaagtgat
    20161 ttcatcatta tctgaaaaaa taattttcac agtactacgc caatcacctg gcattgttgt
    20221 tgttattgga ttggaaattt gaatctcctt aatatctgaa tatgaatttt tcaatgcttt
    20281 gactagacta acctcatatt ctcgattccg ttttagttct tcttttcttt gttgttcttt
    20341 catataaaa
//
LOCUS       DATRSG010000034        20182 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRSG010000034 DATRSG010000000
VERSION     DATRSG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20182
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.034"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..128)
                     /locus_tag="VBD89_001217"
     CDS             complement(<1..128)
                     /locus_tag="VBD89_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7135753.1"
                     /translation="MVSSDNTNLKFLKAFSELLKMRSFEQIKVSDLAKKARLSRRS"
     gene            311..643
                     /locus_tag="VBD89_001218"
     CDS             311..643
                     /locus_tag="VBD89_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO7135754.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
     gene            complement(764..1908)
                     /locus_tag="VBD89_001219"
     CDS             complement(join(764..1674,1674..1908))
                     /locus_tag="VBD89_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
                     /protein_id="HEO7135755.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYHLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNKRKI
                     EGTLLFHSDQGSQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KHYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEENYFNAIK"
     gene            2041..2178
                     /locus_tag="VBD89_001220"
     CDS             2041..2178
                     /locus_tag="VBD89_001220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135756.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2188..2443
                     /gene="mobC"
                     /locus_tag="VBD89_001221"
                     /pseudo
     CDS             2188..2443
                     /gene="mobC"
                     /locus_tag="VBD89_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2418..2913)
                     /locus_tag="VBD89_001222"
                     /pseudo
     CDS             complement(2418..2913)
                     /locus_tag="VBD89_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(3107..4315)
                     /locus_tag="VBD89_001223"
     CDS             complement(3107..4315)
                     /locus_tag="VBD89_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7135757.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYSNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            4696..6360
                     /locus_tag="VBD89_001224"
     CDS             4696..6360
                     /locus_tag="VBD89_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO7135758.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            6449..7372
                     /locus_tag="VBD89_001225"
     CDS             6449..7372
                     /locus_tag="VBD89_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7135759.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRNVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            7374..8291
                     /gene="srtC1"
                     /locus_tag="VBD89_001226"
     CDS             7374..8291
                     /gene="srtC1"
                     /locus_tag="VBD89_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO7135760.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            8248..9099
                     /gene="srtC2"
                     /locus_tag="VBD89_001227"
     CDS             8248..9099
                     /gene="srtC2"
                     /locus_tag="VBD89_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO7135761.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            9181..11853
                     /locus_tag="VBD89_001228"
     CDS             9181..11853
                     /locus_tag="VBD89_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO7135762.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGND
                     GSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDN
                     YISNKFYNTNNRTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIK
                     VNKDKHSESLLGAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYE
                     ISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPP
                     GVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL"
     gene            11907..12697
                     /locus_tag="VBD89_001229"
                     /pseudo
     CDS             11907..12697
                     /locus_tag="VBD89_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            12946..13551
                     /locus_tag="VBD89_001230"
     CDS             12946..13551
                     /locus_tag="VBD89_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO7135763.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            14072..14262
                     /locus_tag="VBD89_001231"
                     /pseudo
     CDS             14072..14262
                     /locus_tag="VBD89_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883557.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
     gene            14649..14825
                     /locus_tag="VBD89_001232"
     CDS             14649..14825
                     /locus_tag="VBD89_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135764.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            16170..16439
                     /locus_tag="VBD89_001233"
     CDS             16170..16439
                     /locus_tag="VBD89_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135765.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            16500..17651
                     /locus_tag="VBD89_001234"
     CDS             16500..17651
                     /locus_tag="VBD89_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7135766.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            17851..18843
                     /locus_tag="VBD89_001235"
                     /pseudo
     CDS             17851..18843
                     /locus_tag="VBD89_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            18846..19664
                     /locus_tag="VBD89_001236"
     CDS             18846..19664
                     /locus_tag="VBD89_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO7135767.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            19666..>20182
                     /locus_tag="VBD89_001237"
     CDS             19666..>20182
                     /locus_tag="VBD89_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO7135768.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWIVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWT"
BASE COUNT         6751 a         3185 c         3951 g         6295 t
ORIGIN      
        1 aaagatcttc tactaagtcg agcttttttt gctaaatcgc taactttaat ttgctcaaag
       61 ctcctcatct ttaacaattc tgagaaagct tttaaaaatt ttaaattagt gttatctgaa
      121 cttaccaata tactatcacc tttatataaa atcttaaagt tataaatctt atcgaatgtg
      181 attatacaat acaaattcaa ataatagttc acatttatcg agataatgta aaacagtaaa
      241 actttaaaaa aagtaaaaat agataacttt attggttaat ctatttttac ttttgaaaag
      301 gagagaagtt atgcttagga agtataagca aagaaattat aatgtcgcac atacgaaaca
      361 gcgtttttcg attaagaaat tcaaatttgg tgtagcatcg gtattaattg gattgacatt
      421 tctaggaatg agtagtcatt ctgttttggc ggatgagtca agcattatga gtactgaggt
      481 accacaaaca gctggtgtaa aaccggaagt tactgttaca cagcctactt cagatgtaac
      541 aagtcttgcg acagatacgt caacttcagc agtgttatca acagatgcat ctcattctaa
      601 tcctacagaa tcagcggagc atgctgtagg attagaatgc tgaagatgca gcggttccag
      661 tgacacacga aaaagaagca aataaaacat tgcgagttac ttcttcttcc acatctgaat
      721 tcacagagtg gactaatgtc aaataagtag acagaaaacc gtgttatttt attgcgttaa
      781 aataattttc ttctttctga ttaggggtta gtcctagatt agccgtatgt gggttgtaat
      841 tgttataaaa attctcaatg tattcaaagc agtctaattg aacctgtttg atattttgat
      901 aatgttttcg gttgatttgt ctatgcttta aatacttgaa aaatgcttca gttacggcat
      961 tatcataagg atatccaggt ttagaaaaag aatgcatgat attgttgtca tcaattattt
     1021 ttctaaattc cctggcctta aattgtgacc cttggtcgga atgaaagaga agtgttcctt
     1081 caatctttct tttattaaga gctaattcta gagtgttaca tgctaacttt gcatccatac
     1141 ggtgactcaa tttccaagca atacattttc tagaatagag atcaagtatt gcgcagagat
     1201 agacatattt cttatatcca atagaaatat aggtgaagtc agtagaccat acttgattag
     1261 gtttgtcagg ataaaatttc tggttgagta agttttgagg atacgttttt tgagtctttt
     1321 taagagctgt ttttggctta acggtagcca ttttagggag cgccatattc ttcatcagat
     1381 ggtaaattct tccttcagag atttttgtgt cgtagtctct ttgaagaatg actttgatag
     1441 acttcacacc aagtcttttg tttgctttgg tataaatctc aagtatttgt tttttataaa
     1501 tttgattatc caattctctt tttgagggct tatgttttag aaatttatag taagtggagc
     1561 gattgacacg taaaacacga cacaaggttg ttgtggcgtg ttcaaagcgt aaccgataga
     1621 cagcgaggag tcttacttta agttttgcat gaatatggca cttgcttttt ttaggataag
     1681 gttctcctct tcaagctgcg cattacgttt ttgtaactct tgtatttgct tggcagtaag
     1741 tacagaatta tcttcgagtt tgaattgaga gtactgtttg atccattttg caagcgcaga
     1801 agtggatacc ctatagtctt tacagagttc agactgagtt tttccagttt gataaaggtt
     1861 aacaagagat tgtttgaatt cttcatcata gcgtttaaat gttgacatag gttgtccttt
     1921 cgtttttgtg tcttttaaaa cagattgtaa cacacaaagt tttgtctact tttatagtat
     1981 acatccaccc tttggcaata ccaaaagttt gagcagatta gttgagatgt gcaggaagtt
     2041 ttgattatcg caagagagaa tcatcaagtg actcatgaac acgtgtcaat cttattgacc
     2101 tgcgttcaag aattgattgt ggaagtcaat caaacacagc cactcagtcg tgagtttcgt
     2161 gaaaagtata tgggttagga ggctgcaatt gtttatcgtt atcgtaccaa tctcaaaaaa
     2221 gtctttctaa cagattcaga attgaatcaa ttaaatgagc ggattgctaa gagcaattgt
     2281 caaaatttct cagtctatgc tagaagagtt cttcttaatc ccaatatgtc atttgtcacg
     2341 attaacacgg attggaaata acatcaatca aattgcgcgt gctatcaacc aaagccatct
     2401 aatttctcag gctcagtatc actttcatcg agaagcacgt taagcccaac agcagggggg
     2461 gttgctctga ttcggttaag taataagcaa aatcctcacc aattttccca gtaataagag
     2521 gcgttgttga ggtataggga ttacctgtgc catagtcgat gatggtcaca aaatgaccat
     2581 tccccataaa agagccaacc aaaacttcac ctgtggccgt tttttgatgt caacaccagt
     2641 attttggatg taccccttaa catgcccttt ggtatctgca acagagataa tatgaccaaa
     2701 ggaagaatca ccgatgactt taaccgtaat cttgctatcg cctttttgat tagccgctaa
     2761 tatttgatta gcaatcaggg tgcgaccaag agcaactgtt gaagacgaca aggtatgatg
     2821 tttttcctga gccaatttaa cagtctcggt gctatcgagg acataagacc tgaaggcacc
     2881 agaggcagaa attgatttaa taattttatc cataacctat gttatagcac aaagagagag
     2941 tttgtatgga ctggattagc tgaaaatttt agactaaaaa gtagtgccag tggattttga
     3001 aaggtaacaa aagagtcttc cgtttaaacg agaaggctgt ttttagtcgt ttcagatgaa
     3061 ggcaaaagga cgatgaaata attccgtacc ttcatggatt gctatgttac tggcatgagg
     3121 tctcacgata tttagtaaga tattcgtttg aagatattcc cacgtatttt ttaaaggttt
     3181 taagaaaatg tgtggtgtcg taaaaatgta ataatttcgc tacttcccca atcggtaccc
     3241 ctctttgcag tagtaattgt ccctccttaa tttttgcctt tagaatataa ctttgcaagg
     3301 aaatgtcaga gtatttttta aaaactgagc gtaagtggga atctgagata tataggtagt
     3361 tggcaatatc agatactttg agtttggagt agaggtggtt gtccacataa tggagaatac
     3421 tattgtacat ttgctgcttg tcagagatgc tcttattggt taaggattct gaaaaatcaa
     3481 taagagctgc acagcgaatt cttgaaacat caataagatc aggagcctct tcgtttaaag
     3541 ccatatagtg ctttaccagc gcataacttt tagccacatc agtattttcc tcgaaactta
     3601 attctagtaa ttttgttaag taaacaacag ttaagttctt ttcagctctt agggcaggga
     3661 ttgaagatga ggtaacactg gatgatggga ggcgattaat ttcttgcttt aacagttgag
     3721 tgttacccag cttaacgaga tcaataatgt gattgagatg gtttaaaaca gtgggtaact
     3781 gaaaagagtt tttcttagta tgttttaggt gaagaacaat atcaggatcc gcaacaatct
     3841 gttctgactc ttctaataaa tgattgatga cttgttggca actagcctca aactgtgttt
     3901 ggaaaaaggc atcgatagac acaagaagac tacgtatact ggtagtagga aaacaaggga
     3961 caagctttat ataggataag atttcttttt tactacgatg agaaaattgt tctagaaagc
     4021 gactggataa ctgttcttgc ctattgatat cagggctata gggataaaat ggtccaatag
     4081 caataagata ttgacagaca ggaaaaatta agaatgattc ttcaaaaaga ccctcataaa
     4141 cagtgatatc ttggttataa gggatagcta aatgttttaa aaactgatag taaggcaaca
     4201 gatagtcttc gttactatat aactgaacga gttccttgtc tcgtgacatg actgaaatag
     4261 gtagttgaga tatggtatgc aatgtttgaa catgtttaaa atcgaatgta accatttgat
     4321 agaccgcctt cattatcatt tctagaattt ttctttaggt ttgtaaagac tacaaaataa
     4381 aatgatgaaa acaactatct tgtggataca ctaaaaagac acgctaatta gcaaactctc
     4441 tcttcatcat ctctcaccat tattatacta ctatttatat gacaaataaa ggtgattttg
     4501 ttaaaatata actttgaaaa tccacatata tttttaatct tccgtctgaa aaaataaata
     4561 aaaatagtaa aaataaacac gaatttaaaa taagcaaatt ttttaagaaa atctgtgcta
     4621 aactttaata gttttgtgct taataataat cagcacttac aaagaacaaa gggaaaagcg
     4681 aggagagaac ttttaatgaa attatcgaag aagttattgt tttcggctgc tgttttaaca
     4741 atggtggcgg ggtcaactgt tgaaccagta gctcagtttg cgactggaat gagtattgta
     4801 agagctgcag aagtgtcaca agaacgccca gcgaaaacaa cagtaaatat ctataaatta
     4861 caagctgata gttataaatc ggaaattact tctaatggtg gtatcgagaa taaagacggc
     4921 gaagtaatat ctaactatgc taaacttggt gacaatgtaa aaggtttgca aggtgtacag
     4981 tttaaacgtt ataaagtcaa gacggatatt tctgttgatg aattgaaaaa attgacaaca
     5041 gttgaagcag cagatgcaaa agttggaacg attcttgaag aaggtgtcag tctacctcaa
     5101 aaaactaatg ctcaaggttt ggtcgtcgat gctctggatt caaaaagtaa tgtgagatac
     5161 ttgtatgtag aagatttaaa gaattcacct tcaaacatta ccaaagctta tgctgtaccg
     5221 tttgtgttgg aattaccagt tgctaactct acaggtacag gtttcctttc tgaaattaat
     5281 atttacccta aaaacgttgt aactgatgaa ccaaaaacag ataaagatgt taaaaaatta
     5341 ggtcaggacg atgcaggtta tacgattggt gaagaattca aatggttctt gaaatctaca
     5401 atccctgcca atttaggtga ctatgaaaaa tttgaaatta ctgataaatt tgcagatggc
     5461 ttgacttata aatctgttgg aaaaatcaag attggttcga aaacactgaa tagagatgag
     5521 cactacacta ttgatgaacc aacagttgat aaccaaaata cattaaaaat tacgtttaaa
     5581 ccagagaaat ttaaagaaat tgctgagcta cttaaaggaa tgacccttgt taaaaatcaa
     5641 gatgctcttg ataaagctac tgcaaataca gatgatgcgg catttttgga aattccagtt
     5701 gcatcaacta ttaatgaaaa agcagtttta ggaaaagcaa ttgaaaatac ttttgaactt
     5761 caatatgacc atactcctga taaagctgac aatccaaaac catctaatcc tccaagaaaa
     5821 ccagaagttc atactggtgg gaaacgattt gtaaagaaag actcaacaga aacacaaaca
     5881 ctaggtggtg ctgagtttga tttgttggct tctgatggga cagcagtaaa atggacagat
     5941 gctcttatta aagcgaatac taataaaaac tatattgctg gagaagctgt tactgggcaa
     6001 ccaatcaaat tgaaatcaca tacagacggt acgtttgaga ttaaaggttt ggcttatgca
     6061 gttgatgcga atgcagaggg tacagcagta acttacaaat taaaagaaac aaaagcacca
     6121 gaaggttatg taatccctga taaagaaatc gagtttacag tatcacaaac atcttataat
     6181 acaaaaccaa ctgacatcac ggttgatagt gctgatgcaa cacctgatac aattaaaaac
     6241 aacaaacgtc cttcaatccc taatactggt ggtattggta cggctatctt tgtcgctatc
     6301 ggtgctgcgg tgatggcttt tgctgttaag gggatgaagc gtcgtacaaa agataactaa
     6361 ataaaaggct acttcttaag taaccatgtt taagaaaaga gaaatagcct tatttctctt
     6421 tttgtcgttt ttaaaataaa ggaacatcat gaaacaaaca ttaaaactta tgttttcttt
     6481 tctgttgatg ttagggacta tgtttggaat tagccaaact gttttagcgc aagaaactca
     6541 tcagttgacg attgttcatc ttgaagcaag ggatattgat cgtccaaatc cacagttgga
     6601 gattgcccct aaagaaggga ctccaattga aggagtactc tatcagttgt accaattaaa
     6661 atcaactgaa gatggcgatt tgttggcaca ttggaattcc ctaactatca cagaattgaa
     6721 aaaacaggcg cagcaggttt ttgaagccac tactaatcaa caaggaaagg ctacatttaa
     6781 ccaactacca gatggaattt attatggtct ggcggttaaa gccggtgaaa aaaatcgtaa
     6841 tgtctcagct ttcttggttg acttgtctga ggataaagtg atttatccta aaatcatctg
     6901 gtccacaggt gagttggact tgcttaaagt tggtgtggat ggtgatacca aaaaaccact
     6961 agcaggcgtt gtctttgaac tttatgaaaa gaatggtagg actcctattc gtgtgaaaaa
     7021 tggggtgcat tctcaagata ttgacgctgc aaaacattta gaaacagatt catcagggca
     7081 tatcagaatt tccgggctca tccatgggga ctatgtctta aaagaaatcg agacacagtc
     7141 aggatatcag atcggacagg cagagactgc tgtgactatt gaaaaatcaa aaacagtaac
     7201 agtaacgatt gaaaataaaa aagttccgac acctaaagtg ccatctcgag gaggtcttat
     7261 tcccaaaaca ggtgagcaac aggcaatggc acttgtaatt attggtggta ttttaattgc
     7321 tttagcctta cgattactat caaaacatcg gaaacatcaa aataaggatt agcatgggac
     7381 aaaaatcaaa aatatctcta gctacgaata ttcgtatatg gatttttcgt ttaattttct
     7441 tagcgggttt ccttgttttg gcatttccca tcgttagtca ggtcatgtac tttcaagcct
     7501 ctcacgccaa tattaatgct tttaaagaag ctgttaccaa gattgaccgg gtggagatta
     7561 atcggcgttt agaacttgct tatgcttata acgccagtat agcaggtgcc aaaactaatg
     7621 gcgaatatcc agcgcttaaa gacccctact ctgctgaaca aaagcaggca ggggtcgttg
     7681 agtacgcccg catgcttgaa gtcaaagaac aaataggtca tgtgattatt ccaagaatta
     7741 atcaggatat ccctatttac gctggctctg ctgaagaaaa tcttcagagg ggcgttggac
     7801 atttagaggg gaccagtctt ccagtcggtg gtgagtcaac tcatgccgtt ctaactgccc
     7861 atcgagggct accaacggcc aagctattta ccaatttaga caaggtaaca gtaggtgacc
     7921 gtttttacat tgaacacatc ggcggaaaga ttgcttatca ggtagaccaa atcaaagtta
     7981 tcgcccctga tcagttagag gatttgtacg tgattcaagg agaagatcac gtcaccctat
     8041 taacttgcac accttatatg ataaatagtc atcgcctcct cgttcgaggc aagcgaattc
     8101 cttatgtgga aaaaacagtg cagaaagatt caaagacctt caggcaacaa caatacctaa
     8161 cctatgctat gtgggtagtc gttggactta tcttgctgtc gcttctcatt tggtttaaaa
     8221 agacgaaaca gaaaaagcgg agaaagaatg aaaaagcggc tagtcaaaat agtcacaata
     8281 attcgaaata ataaaatcag aaccctcatt tttgtgatgg gaagtctgat tctcttattt
     8341 ccgattgtga gccaggtaag ttactacctt gcttcgcatc aaaatattaa tcaatttaag
     8401 cgggaagtcg ctaagattga tactaatacg gttgaacgac gcatcgcttt agctaatgct
     8461 tacaatgaga cgttatcaag gaatcccttg cttatagacc cttttaccag taagcaaaaa
     8521 gaaggtttga gagagtatgc tcgtatgctt gaagttcatg agcaaatagg tcatgtggca
     8581 atcccaagta ttggggttga tattccaatt tatgctggaa catccgaaac tgtgcttcag
     8641 aaaggtagtg ggcatttgga gggaaccagt cttccagtgg gaggtttgtc aacccattca
     8701 gtactaactg cccaccgtgg cttgccaaca gctaggctat ttaccgactt aaataaagtt
     8761 aaaaaaggcc agattttcta tgtgacgaac atcaaggaaa cacttgccta caaagtcgtg
     8821 tctatcaaag ttgtggatcc aacagcttta agtgaggtta agattgtcaa tggtaaggat
     8881 tatataacct tgctgacttg cacaccttac atgatcaata gtcatcgtct cttggtaaaa
     8941 ggagagcgta ttccttatga ttctaccgag gcggaaaagc acaaagaaca aaccgtacaa
     9001 gattatcgtt tgtcactagt gttgaagata ctactagtat tattaattgg actcttcatc
     9061 gtgataatga tgagaagatg gatgcaacat cgtcaataac gatgttgtga atggcttact
     9121 tacttatcaa ataggtgact aatgatgatt gtgaataatg gttatctaga agggagaaaa
     9181 atgaaaaaga gacaaaaaat atggagaggg ttatcagtta ctttactaat cctgtcccaa
     9241 attccatttg gtatattggt acaaggtgaa acccaagata ccaatcaagc acttggaaaa
     9301 gtaattgtta aaaaaacggg agacaatgct acaccattag gcaaagcgac ttttgtgtta
     9361 aaaaatgaca atgataagtc agaaacaagt cacgaaacgg tagagggttc tggagaagca
     9421 acctttgaaa acataaaacc tggagactac acattaagag aagaaacagc accaattggt
     9481 tataaaaaaa ctgataaaac ctggaaagtt aaagttgcag ataacggagc aacaataatc
     9541 gagggtatgg atgcagataa agcagagaaa cgaaaagaag ttttgaatgc ccaatatcca
     9601 aaatcagcta tttatgagga tacaaaagaa aattacccat tagttaatgt agagggttcc
     9661 aaagttggtg aacaatacaa agcattgaat ccaataaatg gaaaagatgg tcgaagagag
     9721 attgctgaag gttggttatc aaaaaaaatt acaggggtca atgatctcga taagaataaa
     9781 tataaaattg aattaactgt tgagggtaaa accactgttg aaacgaaaga acttaatcaa
     9841 ccactagatg tcgttgtgct attagataat tcaaatagta tgaataatga aagagccaat
     9901 aattctcaaa gagcattaaa agctggggaa gcagttgaaa agctgattga taaaattaca
     9961 tcaaataaag acaatagagt agctcttgtg acatatgcct caaccatttt tgatggtact
    10021 gaagcgaccg tatcaaaggg agttgccgat caaaatggta aagcgctgaa tgatagtgta
    10081 tcatgggatt atcataaaac tacttttaca gcaactacac ataattacag ttatttaaat
    10141 ttaacaaatg atgctaacga agttaatatt ctaaagtcaa gaattccaaa ggaagcggag
    10201 catataaatg gggatcgcac gctctatcaa tttggtgcga catttactca aaaagctcta
    10261 atgaaagcaa atgaaatttt agagacacaa agttctaatg ctagaaaaaa acttattttt
    10321 cacgtaactg atggtgtccc tacgatgtct tatgccataa attttaatcc ttatatatca
    10381 acatcttacc aaaaccagtt taattctttt ttaaataaaa taccagatag aagtggtatt
    10441 ctccaagagg attttataat caatggtgat gattatcaaa tagtaaaagg agatggagag
    10501 agttttaaac tgttttcgga tagaaaagtt cctgttactg gaggaacgac acaagcagct
    10561 tatcgagtac cgcaaaatca actctctgta atgagtaatg agggatatgc aattaatagt
    10621 ggatatattt atctctattg gagagattac aactgggtct atccatttga tcctaagaca
    10681 aagaaagttt ctgcaacgaa acaaatcaaa actcatggtg agccaacaac attatacttt
    10741 aatggaaata taagacctaa aggttatgac atttttactg ttgggattgg tgtaaacgga
    10801 gatcctggtg caactcctct tgaagctgag aaatttatgc aatcaatatc aagtaaaaca
    10861 gaaaattata ctaatgttga tgatacaaat aaaatttatg atgagctaaa taaatacttt
    10921 aaaacaattg ttgaggaaaa acattctatt gttgatggaa atgtgactga tcctatggga
    10981 gagatgattg aattccaatt aaaaaatggt caaagtttta cacatgatga ttacgttttg
    11041 gttggaaatg atggcagtca attaaaaaat ggtgtggctc ttggtggacc aaacagtgat
    11101 gggggaattt taaaagatgt tacagtgact tatgataaga catctcaaac catcaaaatc
    11161 aatcatttga acttaggaag tggacaaaaa gtagttctta cctatgatgt acgtttaaaa
    11221 gataactata taagtaacaa attttacaat acaaataatc gtacaacgct aagtccgaag
    11281 agtgaaaaag aaccaaatac tattcgtgat ttcccaattc ccaaaattcg tgatgttcgt
    11341 gagtttccgg tactaaccat cagtaatcag aagaaaatgg gtgaggttga atttattaaa
    11401 gttaataaag acaaacattc agaatcgctt ttgggagcta agtttcaact tcagatagaa
    11461 aaagattttt ctgggtataa gcaatttgtt ccagagggaa gtgatgttac aacaaagaat
    11521 gatggtaaaa tttattttaa agcacttcaa gatggtaact ataaattata tgaaatttca
    11581 agtccagatg gctatataga ggttaaaacg aaacctgttg tgacatttac aattcaaaat
    11641 ggagaagtta cgaacctgaa agcagatcca aatgctaata aaaatcaaat cgggtatctt
    11701 gaaggaaatg gtaaacatct tattaccaac actcccaaac gcccaccagg tgtttttcct
    11761 aaaacagggg gaattggtac aattgtctat atattagttg gttctacttt tatgatactt
    11821 accatttgtt ctttccgtcg taaacaattg taaggtgtcg ttgaaattat taaataatag
    11881 aaaatgacta gttttggcct ttccctattg tcagtcagat tagttattat caagcttctc
    11941 atgccaatat taatgccttt aaaagaacag ttacaaccat tgaccgtacg gagattcaac
    12001 gacggttagg tctggctaaa gcctacaatg ctagtatttc tggaacaagt agtcagtcga
    12061 ctcaatctgt gctgagagat tcttattctg aggagcaaaa aaggcagggt ggactgaata
    12121 cgctaggatg ttagaagtca gagagcaggt tgaccatgtg atgattccaa aaatcaatca
    12181 ggatttacca atctacgctg gttcagaaga ggacaatctg caacggggag ttggtcatct
    12241 agaagggata agtttgccga ttggaggggc ttctacacat gcggtcttga gcggtcaaag
    12301 aggtatgcca gctgctcggt tgtttgcgga tttggataag atgaaaaaag gtgattattt
    12361 ttatgttacc aatctgaaag aaaccttggc ttatcaagtg gatcgtatca tggtgattga
    12421 acctagccaa ttggatgccg tgagcattga agaggataaa gattatgtta cccttctgac
    12481 ctgtacacct tatatgggct ctttgtcaac tgtaatgggt gacttatcat taacaacgag
    12541 agagaatcag cttggttctc tctctttttg gatgttcaaa gcgatgagaa ttttgctttt
    12601 aaaattttta aagttgcgaa agccaaaggc ttgtcgcttg atgtctttga taagcttgtt
    12661 ggtcgcttct aatttggcat tggaaaggga tagctgaggg cgtttgtgat gtaagttttg
    12721 tacttcatga aggtcctaaa gaccatttta aaagcagaat tgccagttgt tacgttatca
    12781 tctatgaagc taaagaattc agttgttcgc ttttcttaga aatgaaagag tatgagctga
    12841 taaaggtcgt tataatagcg gagctcatct gagaaaactt ttatacctca aagtcagtct
    12901 agctttgata tcatgaagcc attaggagtt attccttatc ttttagtggc gcgcgatcca
    12961 tatagtgata gatcgagata tttagatcca aaagttctat catcctcttt tggcgccttt
    13021 tttccagcag ataatattaa ggtagcttgg tctaacaact ccagcacttt atttacacca
    13081 cctattaatg caaactacac cactcagatt caagctattg ggacaacgat taagtcacaa
    13141 attccggaat cgattttgac ggttacggat aaaaagagca ggaagttcag cattaacaag
    13201 attgacgaag ctaaagaagg cttagtaggt gcgaccttca ccttgtctaa acgcacaaca
    13261 gtagcggcag atcatcaagt acaaggagat ttcattcctg tcagcaaaga gacgacagtc
    13321 ggtcggacaa cccttacctt tgacaacctt aaacctggat tttatgacct taaagaaacg
    13381 aaagcgccga atgcttacgt acttgatcct aagacttatg ttgtggtcgt tcaaaattca
    13441 ggaaaaacga caattgtgga tgaagctaac ttcaaagagg ctgattaccc aatggctgat
    13501 aataccagcc atgtggagtg cgtagcgttg ctacaacgaa gcaaagggta aaaatccttt
    13561 attttaagca ctttttcaag cattttgtct ttattgaaaa gagtgatttt aacataaaaa
    13621 aggtattaaa aaacatattg acgtgaccgt ttgttttgaa gtggcttgcg tagacaaaaa
    13681 aatagatacg tcagataaat ttctggcatt acgagaacat ttttagagtg ttctcttttt
    13741 ttagtttacg gaggaaaaat atatatggaa aaacaggatt cacgagttct catccattgg
    13801 gaggggaatt ctggggacaa gctcattgaa caccaaacca gcgcaacggg gtggtactac
    13861 caagtcgatc gtagctttag tcaaccaaaa ggggaaccgc ccagaatgat ccaaaggcac
    13921 tagaaagtgt ccgtaatgat tcgatttcgg gcggtgatga tgtcatgggt tatgcttata
    13981 gcaaatgtac ttggggagtt gcggcacgaa ttaatcagtg ggactgaaac tcaaaggttg
    14041 aaatggtgag aagattacca ttaccagttc aatgggaaat ggtcaggatt gggttggaac
    14101 agccgaaaga ctagatggtg aaactgatac agttccaaaa gaaggtacta ttctctcttt
    14161 ttaggaaagt agttatggtt cgtatatagg ctacggaact atatctttcg tcacattaca
    14221 tctacagata gtaccatgaa ttttgcttat atgaccaagt aaagtgagga tatactaaca
    14281 aatgaaatat ttattatcgt atttgtccat tttatcgaaa agtttgcata ttatcattat
    14341 gtttgataag atgcaaatat aatgatagta ggagctaaat atggatattt aaaaaatcaa
    14401 gagtatccta agtgctttcc attttgaaat tcaaatatag ctaatagttc tagaacttct
    14461 aattgttttt cgtcgacgat atgaattttc aatcttaact gttaggattc cacctccctt
    14521 tggttaaaga aaaaaggtca ggtcgtttag ataactttgt caaacaagct caagctatct
    14581 aaaaatagtt tgaaatgggc attactctag tttttaataa gctatctgat gagcagaagg
    14641 agaagttaat gcatgttggg aagtcttatt ttgactatca agaaaatgct cttatcccac
    14701 aattaggttt tctatattct aaattaacta aaaaaattga acttgataat cggttgtctc
    14761 cgactgaaca aaagttattg attaccttat tattacatac taaaggttta atcattgata
    14821 tgtaagaagt aagtcagcta accgatcttt ctattctaaa acttatattg ttgctttaga
    14881 aattttaaag agcgtggatg gcttcataat aaacagaaat cttaccaatt tgcgaagcca
    14941 aaaaatatga tatttgaaga gtccaaagat ctaatagata gtccagttag agaagcgttg
    15001 attataagtg ataaggattt tcaaaaatta aaacaagagc tattatttta accgacttat
    15061 tttaaagact tatcatatct aggcttgctt gatgattcgg aaaaatacgg agactatact
    15121 atttcaagga aaagatacaa aagtttcgaa tcaagtcttc aactatacat ccttcaaagt
    15181 catcggctag agatttggaa ttatgaacca atccctttga ttactagaaa aataaatagc
    15241 ttggagaggc taacgtgaca ctggttgatc caatctcgct ttatttaaca ctaagaatga
    15301 tgaagaccct cgtattgaag aagaagttga gcagctagaa gataagattt tgagaatata
    15361 atcgtttttt cgtgctacac taacttgtca gggttgcaac agacaaaaaa ataatttagg
    15421 agggattcgt tatgcgtaca aagtatatag gatatgaaat tgttttaagg ggtggttatt
    15481 aatatggata agaaaaaggt tgtttcaact atcgtatcta cagggtttct tatagtttca
    15541 atatcaaatt tttttagaga gctatcacct atactttttc tcttgaatat tattggattt
    15601 ttctgcttcc tattattatt ttctgccaac ataaataaat tgttcttaaa acgatagccg
    15661 ttatatagga tatggtatgt atgcatttgg tgaaattata ggtaaaacga atggaatagt
    15721 acttactcct caaagctatc gtgagagata taaaaggata tcgaggagtc actagatagt
    15781 tggatcacat tgaaaaaatt tgtttaaagt atctcatata agcataaaac gaggaatcaa
    15841 tcaaaatttc ttgattgatt ccttgttttt tggctctttg tcaactgtag tgggttgatg
    15901 aaaaattata ttctggagaa aaccaaattg gttttctcct ttttgatatt caaagtgata
    15961 tgaattctct ttttaaaata ttcaaagttt ctaaagctaa atgtttgtgc ttgatgattt
    16021 tgatgagatt attggttgct caagtttggt gtttgaataa ggcgattcaa ggacattgat
    16081 aaccttatct ctgtctttaa aaaatgtcct aaaaacagtt taaaagattg gagtgacact
    16141 agagaaagtg ttttaaacag ggtaaagaaa tggtgagctt gcttctcttg aaagtaaagc
    16201 aggaggagtt gatagagttt cataatgttt tttatagaga agcgatatct tcttatcttg
    16261 aaacagaaaa ttttgagaag gctaaagtga ctgaagagaa ttaccaagtt tttttaggaa
    16321 tccgtactta tcagaagagt tataactatc aagatcatag tgagaatcta aaaggaaata
    16381 ttgaggtagc aatgatagga gttgaaaata atattagcga cgaacatatt ttttgctaga
    16441 atatataaaa tgtctttgaa ataatttagc cctgtgttat gatattaagg aggttattta
    16501 tggtacgtaa atttttaaaa tcgctcctat ctctctttct tatagcagta atcgccaccg
    16561 gtatttcagt ggcatgtttc ttttttattc cagaaaataa agggaatatt acaccgattt
    16621 tattgcatcg ctttatgcga aaaaataatg ttaatggaat gatgattgtt agcgataata
    16681 ctggtaaacc gattaccatc tcgcatggga ttaaccgtgg ggaagttgaa acggatattg
    16741 aaaataataa attatttcca atggcttctc tccaaaaact catgacagga attattatcc
    16801 aaagattaat tgatcaagat gtcttgagtg aagacgaccg attgtctcag ttctttcccc
    16861 aagtaaaagg tagtaatagt attaccatcc accaattgtt gacccatacg agtgggttaa
    16921 gagaaaaagg tgttaaggtg tctccttatc taaaaaatga gagagaacaa ttacaatttt
    16981 gcttaaaaca ctataacttt gttaataaga aatcgtggta ttattctaat atcaacttta
    17041 gtttcctaac aggtatagcc actcaagtta cgggaaggac ctacgctgaa cttgttgatg
    17101 atgtgataaa aaatcctttg cgattagatg atacccaaag ttaccaatca gttgttaatc
    17161 atgacttagt tagcccaatg cgaaagaacg gcaagttaaa taaaattaat atctttaatc
    17221 aagtatccac agcatatgga gctggagatt ttttcacaac tccgctgaac ttttgggtgt
    17281 tgatgcggtc tttttctaaa gggtactttt tcccaacgga tgaatatacc aaacatcaaa
    17341 atgatgcgat atcacattat tatggtgggc tttacatgca tggtcgtata gttaattcta
    17401 atggggcctt tttccagtat tcaagttttg gttatgcaga tctaaagacg catgatacaa
    17461 tggtgctttt tatgaataac aaagtctata gtgatgctag tcacgtggca ccaaaagcat
    17521 ttgaatatta tcaaaaattt atgttcctta ataagatttt ccacttagtc ttttactttg
    17581 tattttcttt ctttttaatg cttttaatac gtcattcgtt taggaaaaga aggtataaga
    17641 aacgtctcta aatcacatga ataaaaaaga aggctaagtt ggccttcttt tttattcaat
    17701 attaagcagt aacaccatct aagaaaacgt ttgcattgaa tttttaattg tgctataata
    17761 aatagctgtt aatcattatt atagtttaat aatctaactg tcgagctgat ttaagctata
    17821 aaaatactgt tgagtaaaag aggtattaat atgtcaatga ttgaagtttc tcaccttcag
    17881 aaaaatttta taaaaaccgt taaagcacca gggttaaaag gagcatttca atcatttctt
    17941 cgtcctgaaa aacacacctt tgaagctgtt aaagatttaa catttgatgt gcctaaagga
    18001 caaatttggg gatttattgg agcgaacggt gcaggaaaat ctacaacaat taaaatgtta
    18061 actgggattt taaaaccaac ttcgggcttt tgccgtattg atggcaaatt gccacaagaa
    18121 aaccgacaaa attatgttaa ggatattgga gtcgtttttg ggcaacgtac gtagttgtgg
    18181 tgggatttag ctctacaaga aacctataca gtgttaaaag aaatctatga cgttcctgat
    18241 aaagaatttc gcaaacgcat ggctttttta aatgaggttt tggaactaaa tgactttata
    18301 aaggatcctg ttagaacttt gtctctcgga cagcgtatga gagcggatat cgcagcttct
    18361 ttattacata acccaaaggt tcttttctta gatgaaccca ccattggctt ggacgtttca
    18421 gtgaaagata atatccgacg tgcaatcaca caaattaatc aagaagaaga gacaacgatt
    18481 ttactaacga cacatgacct cagtgatatt gaacaacttt gtcatcgtat ttttatgatt
    18541 gatagaggac aagaaatatt tgatggcaca gtttctcagc taaaagagac atttggaaag
    18601 atgaaaactc tttcttttga tttgcgccca ggacaagagc atatcagctc tagtttaatt
    18661 ggaaagtcgg agattaatat taaacgtaat gacttagttt tagatattca gtatgatagt
    18721 tctcgctacc aaactgctga cataattcag cagacattag cagatttttc tgtacgtgac
    18781 cttaaaatga cagatgcgga tattgaagat atcattcgtc gcttctatag aaatgagcta
    18841 taaggatgaa cagaatttgg agacgttata aaccttttat tagtacagga atccaaggat
    18901 taatcacata tcgtgttgat ttcatccttt ataggatagg tgatgtgata ggagcatttg
    18961 ttgcatttta tctatggaaa gcagttttcg attcctcaag tcaatcctta atccaaggtt
    19021 ttcagctgtc agatatgatt ctctatatta ttatgagttt tgtgactaat ttattaacaa
    19081 gaactgatag ttcatttatg ataggtgacg aagtaaaaga cggttcaatt attatgcgac
    19141 tcttacgtcc agttcacttt gctgcttcct accttttcat ggagattggt tctagatggc
    19201 tcatcttttt aagtataggg gtaccgtttt tgcttgttat tacgggggta cgcctcttct
    19261 tggggactga tttgattcaa gctatagtgt tagtggtatt ttatattatt agcattattt
    19321 tagctttttt gattaacttc ttttttaata tttgttttgg cttctcggca tttgttttta
    19381 aaaatttatg ggggtcaaac ctgttaaaaa attcgttagt cgcatttatg tcggggagct
    19441 tgattcctct tacttttttt ccgaaaatcg ttgctgatat tttaggattt ttaccatttt
    19501 catctttaat ttacacacca gtaatgatta ttattggtaa atatgatggc agtcaaattg
    19561 tgcaagcctt acttttacaa atattttggc taatagtaat ggtagcttta tctcaattaa
    19621 tatggaaaaa agtacagtcg catatcacta tccaaggagg ttgagatgac aaaatatcag
    19681 cgaatgcact ttatttttat taaacaatac atgaagcaaa tcatggagta taaaatagac
    19741 ttttttgtag gcgttttagg agtattttta acacaagggc taaatctttt atttctgaat
    19801 gttctttttc aacatatccc ttctttagaa ggatggactt ttcagcaaat agcttttatt
    19861 tatggttttt ctctcctccc aaaaggaatt gatcaccttt tctttgataa cttgtgggct
    19921 cttggtcaaa ggcttattcg aaaaggagaa tttgataagt atttaaccag gccaatcagt
    19981 cctttatttc atgtcctagt tgaaaccttt caagtagatg cattggggga gttattggtt
    20041 ggttttattc tgttatctac cacagtatct agtataagct ggatcgtgcc aaaagtatta
    20101 ttatttatat ttattatccc ctttgcaact ttgatttata cctccttgaa gattgctacg
    20161 tcaagtatcg ctttttggac ca
//
LOCUS       DATRSG010000035        19722 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRSG010000035 DATRSG010000000
VERSION     DATRSG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19722
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.035"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            86..1303
                     /locus_tag="VBD89_001238"
     CDS             86..1303
                     /locus_tag="VBD89_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7135769.1"
                     /translation="MSEYKTIHPNARLKDQYDNTIGVVSEVYDNTSHSGEQIYAVVDN
                     RHIPKEDVKEVTVLFRGSTSPQEFINNPADVTLDWLENDIPMASNIWVMKDFGNPHNF
                     SAVSPQLKASSEHLKEIMKKYPNAKINIGGHSLGGMDAQYAVVDITDKKDLKRINSVH
                     VYNAPDIYPTLTKGQKKTADSLKSKIVVYVDPNDFIGMVGREGKKGSEDSVGTVYYTE
                     SPDINWIDQHMTYGYRMENGQIKVIETNLPPEVRTTRKAMGSFYKDKKKFKKSGKGLS
                     SHEKIFLDAEQAAVIASGLVSTAETALEDIKTSAKTAVGEAEELWNTTKSIPFGITEL
                     TEAELAEAYAEGGVTYDSIVTKTDTHFDEKVKNAGDLVTTYTTLKSDIQSGVETMLSK
                     DSELAGDFKAWEN"
     gene            1291..1692
                     /locus_tag="VBD89_001239"
     CDS             1291..1692
                     /locus_tag="VBD89_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135770.1"
                     /translation="MGKLDAKDDKLSNSKKDLEKLEDDYHHKTIAISDKFFELEDKRC
                     EFETMLQETYEATSYHLRQDEMINEESFMTMNHIIEAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            1676..1981
                     /locus_tag="VBD89_001240"
     CDS             1676..1981
                     /locus_tag="VBD89_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135771.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRRSIVSTYDAEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
     gene            complement(2170..2242)
                     /locus_tag="VBD89_001241"
     tRNA            complement(2170..2242)
                     /locus_tag="VBD89_001241"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2207..2209),aa:Lys,seq:ctt)
     gene            complement(2524..3792)
                     /locus_tag="VBD89_001242"
     CDS             complement(2524..3792)
                     /locus_tag="VBD89_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO7135772.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDGALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(3785..4276)
                     /locus_tag="VBD89_001243"
     CDS             complement(3785..4276)
                     /locus_tag="VBD89_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7135773.1"
                     /translation="MIIRPMQNCDREAVLTLQNTGWTALTSPVYDHKWTESDLEKNLA
                     NGMSFFVAEIDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            4603..5073
                     /locus_tag="VBD89_001244"
     CDS             4603..5073
                     /locus_tag="VBD89_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO7135774.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGEMSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            5209..5667
                     /locus_tag="VBD89_001245"
     CDS             5209..5667
                     /locus_tag="VBD89_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7135775.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     DGREGDETPLECLFREVSEELNLTLTRNHIDWVKTYRGMLNPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            5639..6109
                     /locus_tag="VBD89_001246"
     CDS             5639..6109
                     /locus_tag="VBD89_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7135776.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(6148..6864)
                     /locus_tag="VBD89_001247"
     CDS             complement(6148..6864)
                     /locus_tag="VBD89_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7135777.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGYDYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            6988..7446
                     /locus_tag="VBD89_001248"
     CDS             6988..7446
                     /locus_tag="VBD89_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO7135778.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYSPWCSDLNRTALVTQRWQLNYFIDLSKNNEGVTT"
     gene            7443..8396
                     /locus_tag="VBD89_001249"
     CDS             7443..8396
                     /locus_tag="VBD89_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO7135779.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIIANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            8396..9136
                     /locus_tag="VBD89_001250"
     CDS             8396..9136
                     /locus_tag="VBD89_001250"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO7135780.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            9133..9719
                     /locus_tag="VBD89_001251"
                     /pseudo
     CDS             9133..9719
                     /locus_tag="VBD89_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
     gene            9920..10801
                     /locus_tag="VBD89_001252"
     CDS             9920..10801
                     /locus_tag="VBD89_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO7135781.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNTKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            10816..11661
                     /gene="pstC"
                     /locus_tag="VBD89_001253"
     CDS             10816..11661
                     /gene="pstC"
                     /locus_tag="VBD89_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7135782.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYRP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            11663..12541
                     /gene="pstA"
                     /locus_tag="VBD89_001254"
     CDS             11663..12541
                     /gene="pstA"
                     /locus_tag="VBD89_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7135783.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAIGRIIGE
                     SAALIFTAGTVAKVVHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            12534..13283
                     /gene="pstB"
                     /locus_tag="VBD89_001255"
     CDS             12534..13283
                     /gene="pstB"
                     /locus_tag="VBD89_001255"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7135784.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            13280..13936
                     /gene="phoU"
                     /locus_tag="VBD89_001256"
     CDS             13280..13936
                     /gene="phoU"
                     /locus_tag="VBD89_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7135785.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            13936..14613
                     /locus_tag="VBD89_001257"
     CDS             13936..14613
                     /locus_tag="VBD89_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7135786.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAVQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            14606..16261
                     /locus_tag="VBD89_001258"
     CDS             14606..16261
                     /locus_tag="VBD89_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7135787.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            16416..18599
                     /locus_tag="VBD89_001259"
     CDS             16416..18599
                     /locus_tag="VBD89_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO7135788.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSIRLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLANSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AATQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            18638..19468
                     /locus_tag="VBD89_001260"
     CDS             18638..19468
                     /locus_tag="VBD89_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO7135789.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLATSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
BASE COUNT         6250 a         3196 c         4187 g         6089 t
ORIGIN      
        1 tattataagt gcagatttag atgatgattt agaaaccaat aaaaaatgaa aaaaatatat
       61 tgatgaagaa aaattacaaa tcgcaatgtc agaatataaa acaatccatc ccaacgccag
      121 attaaaggat caatatgata acacaattgg agttgtttcg gaggtttacg acaatacttc
      181 tcattctgga gaacagattt atgctgtggt tgataatcgt catattccaa aagaagatgt
      241 aaaagaggtg acggtacttt ttcgtggatc aacatctcct caggaattta ttaataatcc
      301 tgcagatgtg actttggatt ggttagaaaa tgacattcct atggcaagta atatttgggt
      361 aatgaaagat tttgggaatc ctcataattt cagtgctgta tcaccacagt tgaaagcttc
      421 atcagaacat ttaaaagaga ttatgaaaaa atatccaaat gcaaaaataa acattggtgg
      481 acattcatta ggaggtatgg atgctcaata tgctgttgta gatattacag ataagaaaga
      541 cttgaaacgg attaactctg tacatgttta taatgctcca gatatatatc ctacacttac
      601 taaaggacag aaaaagacgg cagattcact gaaatctaag attgttgttt atgttgatcc
      661 taatgatttt attggcatgg tggggcgtga aggaaaaaaa ggaagtgaag actctgtagg
      721 aacagtctat tatacagaaa gtccagatat caactggata gatcaacaca tgacctatgg
      781 ctatcgtatg gaaaatggtc agattaaagt tattgaaacg aatttaccac cggaagttag
      841 gactactaga aaagctatgg gatcttttta taaggacaag aaaaaattta aaaaatctgg
      901 taagggtcta agttcgcatg aaaaaatctt cttggatgct gaacaagctg ctgttattgc
      961 aagtggttta gtctctactg ctgaaactgc tttagaggat atcaagacat ctgcgaaaac
     1021 agctgttgga gaagcagaag aattgtggaa tacgactaaa tcgattcctt ttggtattac
     1081 ggagcttaca gaagcagagt tggcagaagc ttatgcagaa ggtggtgtca cttatgatag
     1141 catagtaact aaaactgata ctcattttga tgagaaagtc aaaaatgcag gagatttagt
     1201 aacaacatat acgactttga aaagtgatat tcaatcaggt gttgaaacca tgttatctaa
     1261 ggatagtgaa ttagctggag attttaaagc atgggaaaat tagacgctaa agatgataaa
     1321 ttaagtaaca gtaaaaagga tttagaaaag ttggaagatg attaccatca taaaaccata
     1381 gctatttcag ataaattctt tgaactggaa gataaacgat gtgagtttga gacgatgctt
     1441 caggaaactt acgaagcaac aagttatcat cttcgtcagg atgagatgat taatgaagag
     1501 tcctttatga ccatgaatca cattatagaa gcttttcaaa gtgattttga tacagaatat
     1561 actaaagaga aaagaagatt gactgcctta gaagaagaaa ctaatcaaaa gtactctaaa
     1621 aagagacaat tgcttgaaga aaaaatagat catttaatgt cagaaaggag agactatggg
     1681 aatccttggt gattatgttt tatcagaagc ctctgattat atcagtggaa aaaaagatat
     1741 caaggcttta aaaaagaaac ttgataaaat gttagtgaca ggtgtttatg ctccaagaat
     1801 caagtctaaa cgtagaagta ttgtctctac ctatgatgca gagataacga ctactgcaac
     1861 aaacgcaaaa gctagtataa ctgccattgc aggtcaaata gacactgcaa taaagggaca
     1921 gtttagaact aaagttgaaa ctgtattgga taataacagt acaaagtatg atgaaattta
     1981 aaagacatgg gctaaataat ttagctcttt ttttatactc aaaaataaga gaaatacaaa
     2041 acaaaataca tatttatgca agtaaagaga aataaaaaaa tcaccatttt atctgtcaaa
     2101 aaaaattggt gggcaagtgt aaaaagagcg tcaaaaaagc ctataggtat aggctttgtt
     2161 ttataagact gccccctaca gggctcgaac ctgtgaccca tagattaaga gtctactgct
     2221 ctaccaactg agctaaggag gctaggaagc tgtattggca ccgatgatcc actttttgta
     2281 ttgatcccgc acaaaataag caggtgggta actcgcacct ctttaagtgg cttccgcgtg
     2341 ttaacggctt gcgcactaga gatgacttcg tttcccgaaa tacaaagaaa tagtcggtca
     2401 acacttagat gtggattcgt atgccacagc aatgtttcta tgtctttatg ataccacttt
     2461 ttataaaatt ttcaagcaaa attatcgatt tttgaaatcg attgcgattt ttaaaactgg
     2521 caatcagtct gagagcttat caatgcgctc tttatttgaa ccaagtgcac gttcgtattt
     2581 accagtttca ttggcttcaa aataattatg tcctactagt ttatctggta agtattgttg
     2641 tttaacccat ttttcaggat aggcgtgagg gtacttgtaa tcccttgcat tgcccaaggt
     2701 tttactaccg ctgtaatgtc catctcttag gtgacgtgga ataggtaaat ttcctgaacg
     2761 acgtaaatca gctaaggctg catccatagc caaataggct gaatttgatt taggtgatag
     2821 agctaaatcc acaacaatgt tagcaataag aatacgagct tctggaaaac caatgcgttg
     2881 ggcagcttcc aatgcagtca cagtatgtat ttgtgcttcg gggttagcta agccgatatc
     2941 ttcataagca ataatggtca aacgtcttgc taaactaggt aaatctccag cttctactaa
     3001 acgagcggca tagtgcaggc tggcattgac atcagaacct ctaattgatt tttgtaaggc
     3061 tgataggaca tcataatgcc catctccgtt tttatccatt gtaatataac tacgttgcag
     3121 actattttcc attgtctcta gactaatatg acggctacca tcttcgttag gagaagtaga
     3181 catgactgct agatccaagg aattataagc agatcgtagg tctccatttg tggcggtaac
     3241 aatgaaatct agcgccccat catcaattgt cactaaaaaa ggaaatcccc gttctttatc
     3301 ggaaattgct agttgaatgg cttttttaat atcctcgtta gaaagaggtt ctagctcaaa
     3361 aatttgtacc ctacttctaa tggcaggagt tactgaaaag aaaggatttt cagttgttgc
     3421 tccaatcata ataatattgc cattctcaag taaaggtaaa agaaaatctt gttttgtctt
     3481 gtcaagtcta tgaatctcgt caagtaataa aactaagcca ccagaaaatt tagcttcttc
     3541 tgcaatttct tgaagacgtt ttttactatc aacagtggca ttaaaagtac gaaaggcata
     3601 tttagtagta ccagcaatag cagaagcaat agaagtcttc ccaatacctg gtggtccata
     3661 taggatcatg gaagataaca tattagctgc aaccattcga tcaataattt tgccattacc
     3721 aacgagatgt tgctgcccaa tgacttcatt aatattgcga ggacgcatac gtagagcaag
     3781 attgtcagcc atgattattc tccaaatctt ttgcgaaaat taaagcatca acataatgat
     3841 tttcaatgaa aaaggcttta gtgattcgag cctcttcggt aaaaccgtat ttttgataga
     3901 gggaaatggc tctactattg ttgcccatga cgtgcatggc aatcttgatg taaccttgag
     3961 ctttagcctc agttagcaga gctttcaaga gagccttacc tagcccctga ccttggtagg
     4021 gttcagcgat gagaatgccg aaagtggcaa catgcttgcc agctgggaaa ggatagtagg
     4081 gaccaaagtc caagacgcca gcgatcttat catcgatctc agcgacaaag aaggacatgc
     4141 catttgccag attcttctcc aagtcactct cagtccactt gtggtcatag acaggagagg
     4201 taagagcagt ccagccagta ttttgtaagg tcagaactgc ctcacggtcg cagttctgca
     4261 taggtctgat aatcataatt gatgatacaa agggacggtt cggtttacga aaattttcgt
     4321 aaagaaatag ggaacgacga agtgtcgtca gacactagga agttcatctt ttcttttaga
     4381 aaattagtcc cgtgttcaaa tcctttctat tttttatata gagtttttct ctcagctgac
     4441 tcttttaaca tagtatgaag tcagtcaaaa acgactagtc tttgagacta ttgtaacata
     4501 aaaataagta ggaactacct tttgattaac ctgagtctgt aacgtttttc ttctatattc
     4561 atgttataat aagaaagaat aaatagaaaa taaggacata agatggctaa atttggattt
     4621 ttatcggtac tagaagaaga attggataag catttacagt atgactttgc catggattgg
     4681 gataagaaaa atcatactgt agaagtcact tttatattag aagcacaaaa cagctctgct
     4741 attgaaaccg tggacgacca gggggagatg agtagcgagg acatcgtttt tgaggactat
     4801 gtactctttt acaaccctgt caagtctcgc tttgatgctg aggactatct ggtgactatc
     4861 ccctatgagc ctaagaaggg cttgtcgcgt gagtttctgg cttattttgc ggagactctc
     4921 aacgaggttg ccactgaagg gttgagcgac ctcatggact tcctcaccga tgacagtatc
     4981 gaagagttcg gtctttcttg ggatacggat gcttttgaaa atggcagagc agaactgaaa
     5041 gagacagagt tttaccctta tccgagatat tagacttgat ggatttgcta aaatgtggat
     5101 atacattgga tgatatagaa acagccagac cagaggatat ggaacgttat tatccccctg
     5161 aacaaatcag aaaatatgga gctttaggaa tagaattgag gttattgcat ggatattttt
     5221 gatgagttat ttgatttctc aggtgcaaag atcgctcttt tctgcgaggg aaagattttg
     5281 accagcctaa gagatgattt tccagatttg ccctatgcag gcttttggga cttaccaggc
     5341 gatggtcgtg agggcgatga aacacctttg gaatgtcttt ttcgagaagt tagtgaagaa
     5401 ttaaatctga ccttaactcg aaaccatatt gattgggtca agacttatag gggtatgctg
     5461 aaccctgata aattatcggt ttttatggtt ggacatatca gtcagaaaga gtacgatagc
     5521 attgttctag gcgatgaagg acaagactac aagctcatgt ccattgacga atttttaagc
     5581 cataaaaagg tcattcctca gttgcaggag aggttgagag attatttgga ggtggaagat
     5641 gattacttta caaaaggcgg aagctagtga tttagagaag attattgcca ttcagagagc
     5701 tagctttaag gcagtctatg agaaatacca cgatcaatac gatccctatg tggaagaagt
     5761 tgagcagatt cgatggaaac tagtggagcg accagattgt ttctatcatt ttgtccttgt
     5821 tgatgagaca attgttggtt ttcttagatt ggttatcaag gacgaggaga agcgtgcttg
     5881 gcttggtacg gcggctattc ttccccaata tcaaggacag ggctacggct cagcagcgat
     5941 ggctttatta gagaaaacct atcctaagct tacaaagtgg gatttatgta cgattgcgca
     6001 agaaaaacta atggtcagtt tttatgaaaa atgtggttat cattcaaccc ataccgaacc
     6061 tgagcaagaa ggaatggata tggtctatat gacaaagaag atagaataaa aaaagaaaac
     6121 tagatgattg tctagttttc tgatagttta ttggcaatag taagcaatag cttggctgac
     6181 aaattcactt gttcctgctc caccagcttt gtcaattgat tgactaaacc ggtcatctga
     6241 ttgatacatt tgtcctagtc cagcaaggat ttcattggta caggtgtaaa aatgctgaga
     6301 gatatagtct tgtagccgtt tcacacacat ttgaacagat tcatcagata cattttgtgt
     6361 ctttaattgc ccaaattgta ccattatttt tgccatttct tggctaattt gtgcaaagtc
     6421 atctgaagca tgtttttgag caaattcttg gtaggctgct gtttgtcccc atttttcttt
     6481 tgcttcagct tgtaattgtt ctaattctgt tttattataa acatcgaaat tcatataatc
     6541 atatcctttt tcttgtaatc gtttcgcatg ggtgataacc tgctcaagat gcgatctttt
     6601 ccattcaagc aatttgattt gttgggctaa taagtcttgc ctttcctctt ttgcttgctc
     6661 aagcaggtat ttgatatctt tgagtgggaa ttccaactca cgaaatagca aaatttcttg
     6721 caggcgaatc aatgcttcat cgtcataaag tcgatagcct gcttcggata gggcggtagg
     6781 tgggaataaa ccgattttat catagtgatg taaggtgcgc acacttacac cgcttaagat
     6841 actgacttct ttaactgttt tcatggtaat aacctcctct cacttacata gtataaagga
     6901 taacctaagg tcagggtcaa gcaaaaaata aattattttg aaaagtatgg ttaatcatat
     6961 tgatttgaag ataaaggagg gatagttata gaaatagttg agctactaag ggaatggtat
     7021 tcttcctttg aagttaaaga agaaaagtgg aatgaaaaat atgaaggtat cctcataaga
     7081 attgaaggcg atggaaacta taaaaggtgt cgtttagctc ataagacagc tagcaagatg
     7141 gggtatttta cagtattttg gcagaaagat ggaaatggta aaaatattcc tttttatgaa
     7201 caagatgaag tggaagattt gattattgtc attaaagatg gtaggaggaa aggtttgttc
     7261 atcattccca aagaggttgc agtgagtaag ggtattcttt catctgctaa ctctcaagga
     7321 aagatggcta tgcgatttta ctctccttgg tgtagcgatt taaatagaac agctttggtg
     7381 acacagcgat ggcagttgaa ctattttatt gatttaagta aaaataatga aggagttact
     7441 acatgaacac atggaatgaa ctaacggttc acgtgaatcg tgaggcagag gaagctgtct
     7501 cgaatttatt gattgaaact ggcagtcagg gggttgccat tagcgactcg gctgattatc
     7561 ttggacagga ggatcgtttc ggcgagcttt atcctgaggt ggagcaatct gatatgatcg
     7621 ctattactgc ctattatcct gatacgcttg atattgaggc ggttaaggct gatctggctg
     7681 atcgtttggc taattttgaa ggctttggtc ttgcgacggg tagcgtcaac cttgacagtc
     7741 aggaattggt tgaagaggac tgggctgata actggaagaa gtactatgag ccagctcgca
     7801 ttacccatga tttgaccatc gtgccgtcat ggacggacta cgaggctaag gctggtgaga
     7861 agattatcaa gctggatcct ggtatggctt ttgggactgg gactcaccca acgactaaga
     7921 tgagtctttt tgccttggag caagtgcttc gtggtggtga gacggttatc gatgtgggga
     7981 ctggttctgg cgttttgtct attgctagct ctctcttggg tgccaaggac atctatgctt
     8041 atgatttgga tgatgttgcg gtgcgcgtgg ctcaggaaaa tatcgatatg aaccctggta
     8101 ctgataatat tcatgtggct gctggtgacc tgctcaaagg tgtccagcaa gaggcagatg
     8161 tcattattgc caatattttg gcggatattt tgatccatct gacagacgat gcctaccgtt
     8221 tggtcaagga tgagggttat ctcatcatgt ctggtatcat ttctgaaaaa tgggacatgg
     8281 tgcgtgagag tgctgaaaaa gctggtttct tccttgagac tcacatggtt caaggtgagt
     8341 ggaatgcctg tgttttcaaa aagacagatg atatttcagg agtgattggc ggctaatgca
     8401 acagtatttt gtaaatggca aagcagatga ctttgtgacc atcgaggaca aggataccat
     8461 caagcacatg ttcaatgtca tgcgattgac agaagatgac caagtggtct tggtttttga
     8521 tgatgccatc aagcgtctgg caaaggtggt ggacagctct gcccatcgct tccaaattct
     8581 agaagagcta gacagcaatg ttgaaatgcc tgtccaagtg accatcgcct caggttttcc
     8641 caagggagac aagttggatt ttgtgacgca aaaggcaaca gaattgggtg ctgcggctat
     8701 ctggggattc ccagctgact ggtcagtcgt caagtgggac ggcaagaaac tagctaaaaa
     8761 agaagataag ctggcaaaaa ttgctctggg cgcagcggag cagagcaagc gtaatcgtct
     8821 tcctcaggtt cgtttatttg agaagaaggc tgattttcaa gcggagcttg caggctttga
     8881 taagattttt atcgcctatg aagagtctgc caaggagggt gaaatgtcag cgttagccca
     8941 gaatttgcag actgtaaaag caggtgacaa gctcctcttc atctttggac cagaaggggg
     9001 catttctcca aaagaaatcg cagcttttga agaggttgga gctatcaagg tgggcctggg
     9061 tccacgcatc atgcgcacag aaacagcacc gctctatgct ctaagtgtta ttagttactc
     9121 agcggagtta ttatgattat tgctattcaa ggtgatatgg ctgtagggaa aaccacagct
     9181 ttgaaaatca ttgagaaagt ttacccgaaa atcgctatta gttttgagga tatttcacca
     9241 gtagttgata agattaataa acatcagctt gataacaata aattagacga ttattgtgaa
     9301 attcaacaac tatttattca gcatgagcga gatagatacg aacttgttag agataatgaa
     9361 atagtgttaa tggattatgg agctgaggaa attgctttct atacacaata ctttccaaag
     9421 gtggcgggtt atagttgact ttaccttcta atttgttact agaggtaaat aggctaaagg
     9481 aatgttttgc tgattatatt atttttctag atgcagatga gacggtccta taccaacgtc
     9541 aaaaaaatga ttttaataga cggcgtcatt ttttcaaatg gtatactcat cacctgttgc
     9601 ctgctaaaaa acattattta gacacatttt ccaatgttat tcatattaat acaaatcatt
     9661 taaatgctga agaagtggct gaaaaaatca ttgacatcat tcgtaagtta actctataat
     9721 gggatagtta tacttcaatt atgtggtata acaactgttt caagccgact tattggctga
     9781 attaccataa tagaaaaaag gagtttggat aattttccaa ctctttttaa ttttacattg
     9841 attttacaaa aatcctcaaa gagatttgat attccttaac tataataaaa tcaaatcaaa
     9901 aaatatttag gagttcatca tgaaaaaaca caaaatgcta tctcttttgg cggtatctgg
     9961 tcttatggga attggcatcc tagcaggatg ctcaaatgat tcatcatctt catctaaagg
    10021 gacaattaac attgtttcta gagaggaagg gtctggaact cgtggagctt tcatagagtt
    10081 atttgggatc gagtcaaaaa ataaaaaggg cgaaaaagta gaccatactt ctgatgcagc
    10141 aactgttacc aattcaactt cagttatgct aaccactgtc tctaaagacc cgtcagctat
    10201 tggttattct tcactaggtt cattaaatag tagcgttaaa gtgcttaaaa ttgatggcaa
    10261 aaatgcgact gtaaaagata ttaaatcagg aagctataag atttctcgtc ctttcaatat
    10321 tgtaacaaaa gaaggtaagg aaaaagaagc aacaaaagat tttattgatt atatcttaag
    10381 caaagatggg caagcagtcg ttgagaaaaa tggctatatt ccacttgaca atgctaaagc
    10441 atatcaagca aaagtgtcat ctggtaaagt tgttatagca ggttcaagtt cagtaacgcc
    10501 ggttatggaa aagattaaag aagcctatca taaagtaaat acaaaagtag atgttgaaat
    10561 tcaacaaagt gattcttcaa ctgggattac aagcgcaatt gatggatcag cagatatagg
    10621 tatggcatca cgtgaattgg ataagacaga gtcatccaaa ggggtcaaag ctactgttat
    10681 tgcaacagat ggtattgcag tagttgttaa taagaaaaat aaagttaatg atctatcaac
    10741 aaaacaagta aaagacattt ttactggaaa gacaacgtct tggtctgact tatcaaagta
    10801 aggtaagagt aaacaatgaa acaaacaaaa gaaggtctta tgcaagctgt gtttttcatc
    10861 acggcttgcg tcagtgttat atcagctatt ttaatttgcc tctttctctt ctctagtggt
    10921 cttccagcta ttacaaaaat cggctgggga aattttatat ttggtaaagt ctggcatcct
    10981 agtaataata tttttggcat ctttccaatg attgtaggtt ccttatatgt tacagcggga
    11041 gctttacttt taggaggccc gataggtatc cttactgctg tttttatggc ttatttctgc
    11101 ccagaaaata tttataggcc tttaaagtca gctatcaatt taatggcggg aattccttca
    11161 gttgtttatg gtttctttgg actcgttgtc attgtcccta tgataaggca atatattggt
    11221 ggctttggta tgggagtctt agcagcttca attttgctag gaattatgat tttaccaaca
    11281 attgttagca tttcagaatc ttctttaaga gctgttccag agagctatta tgaaggcggt
    11341 attgcccttg gtgcctctca tgaacgaagt gtcttctttg ctgttttacc agctgctaaa
    11401 cgtggtattt tagcatcggt tgttttggga attggacgtg ctattggtga aacaatggct
    11461 gtcatcatgg tagcgggtaa tcaagctgtc ttgcctcaat cgctgacatc aggcgttaga
    11521 actctaacca ctaatattgt catggaaatg ggatattctt ctggtttgca tcgtcaagcc
    11581 ttaattggta cagctgtggt actctttatc tttatattaa tgattaatat cagtttttca
    11641 gcattacaga aagaggaata agatggaaaa tattaaccaa agaacgtcaa aacaatattt
    11701 agcatcttta aaaaaagatc ccctttcctt ctttcttttt gctattgtct atttgggagc
    11761 aattctaagt tttgctacaa ttgcatttgt tgttatttat attttagtta aggggcttcc
    11821 tcatgtgaat actggcttgt ttgcatggac ttataatact cagaacgtct cactattacc
    11881 agctttcatt aacactatct ttatcatcgc cttaacttta ttatttgcag ttccccttgg
    11941 tattggtgga tcaatttatc taacggaata tgctagacgt gataatcctt atctcaaaat
    12001 tatccgtgtt gcaaccgaga cattggcagg gataccttca attatttatg gtttatttgg
    12061 tgcattattt tttgtaaaat acacacactt aggcttatct ttgatatcgg gttctctgac
    12121 tttgagcatc atgattttac ctttgattat gaggacaact gaagaagctt tactatctgt
    12181 tccggattct tatcgagaag gagcctttgc gcttggtgct ggtaaattga gaaccatatt
    12241 taaaatcgta ttacctagtg ctatgtctgg tatttttgct ggtattattt tagcgatagg
    12301 acgtatcatt ggtgagtcag cagcacttat tttcacagct ggaacagttg caaaagtagt
    12361 acattcggta tttagttcat ctcgtacttt agcagtacat atgtatgcta tttcgggtga
    12421 gggcttatat gttgaccaaa cttatgcgac tgcggtcatc ttattgcttt tagttattat
    12481 tgtcaatttt gtatctggac tagtagccaa acgattagga agtgagtcga acagtgagta
    12541 aattagtaat taataattta gatttgtatt atggagaatt tcacgctctg aaggatgtta
    12601 atttagacat tgaagaaaaa gaaatcacag cctttattgg accttctggg tgcggaaagt
    12661 ctactctctt gaaaagtatt aaccgtatga atgatttggt caaaaattgc aaaatcacag
    12721 gggatattac tttagaaggc gaggatgtct atagacaatt ggatattaac caattgcgta
    12781 aaaaggttgg aatggtgttt caaaaaccta atccgtttcc aatgagcatt tatgataatg
    12841 ttgcttttgg tccaagaacg cacggtattc attccaaagc agagttagat gatatcgttg
    12901 aacgttccct taaacaggcg gctctatggg atgaggttaa agatcgtctt cacaaatctg
    12961 ctctgggtat gtctggcgga cagcagcaac gtttgtgtat tgcgcgtgct cttgctattg
    13021 agcctgatgt tttacttatg gatgaaccca caagtgcctt ggatcctatt tcaacagcaa
    13081 aaattgaaga attggttatc caactgaaga agaactacac tattgttata gtgacacata
    13141 atatgcaaca agctgttcgt atttctgata aaacagcatt ctttctgatg ggagaagtgg
    13201 ttgaatataa caaaacgagc caactctttt cattgccaca agatgagcgt acagaaaact
    13261 acattacagg gagattcgga tgattcgttc acgatttgca agtcaactta atgaccttaa
    13321 taaagaaatt atttttatgg gggcattgtg tgaggacatc attgggaagt ccttaggtgc
    13381 tctaacaaat agtaatgatg tttaccttga cgatatttct gaaacttatc acaagattga
    13441 acaaatggaa cgggatattg aggagagatg tttaaaactt ttacttcgtc agcaaccagt
    13501 tgccaaggat ttacgccgta tttcttctgc attaaaaatg gtttatgata tgaaacgtat
    13561 tggtgctcaa gcttatgaaa ttgcggaaat tgttagttta ggtcatatta tccaaggtgc
    13621 aggttctgaa agggatcaac ttaacagcat gtctaataat gtcattagta tgctgactaa
    13681 gagtatagat gcttttattt atgacaatga agaacaagcg catcaagtaa ttgaacaaga
    13741 taggacagtt aaccaggaat ttgatacaat aaagaaacag ttagtgttat atttttcggt
    13801 acaggatgtt gatggtgaat atcccataga tgttttaatg atcgccaagt acctagagcg
    13861 tattggtgat catacagtaa atattgctaa atgggtgctt ttctcaataa caggtaacct
    13921 agatggagaa gcgtaatgat atattgtgtt gaagatgatg ctgatattcg cgaaatgatg
    13981 ctctatactt tgcaaatggc aggttttaca gctcaaggat tctcgagttc agagcttttt
    14041 tgggaagctg ttcaggagaa agttccagac ttgattttac ttgatattat gttgccggga
    14101 gatgatggtt tgactatttt agagcgttta aggagaaaac atcaaacgga aatgatacca
    14161 gtaatcatga caactgctaa aggtagtgag tatgataaag ttaaagggtt agatcttgga
    14221 gcagatgatt atctcgtcaa accttttggg atgatggaaa tgatttcacg aataaaagca
    14281 gtcttgagac gtagccgcca agtagattca aaagctcata ttatcattgg aaatttagag
    14341 attgacccga ctaattattg ggtcaaaagg ggaacggaaa aaatccactt aaccctaaag
    14401 gaatttgagt tattagtatt attcttccgt aatcccaata gagtctttac aaggcaagaa
    14461 cttctggata aggtttgggg agaacaattt ttaggagaaa ctagaactgt ggatgttcac
    14521 attgggacac tgcgaacaaa acttggtgag gatggctatt tgatcgctac agtaagggga
    14581 gttggatacc gtttggagga aagacatgac taaaaaaatt ttccgtacaa ctttatccgc
    14641 tagtttggga attgttttag ttacaatttt gatgattatg ggatttctct ataattactt
    14701 caatcgcatt cagcgagaac agttgagaac acaaacggca ctggcgtcac aaggaatttc
    14761 ctttgaaggt aaagattatt ttgaaaatct taaaacttca aatgtgcgta ttacttgggt
    14821 agataataag ggacaagtgc tttatgatac ccaatcagat gcaaaacata tgaaaaatca
    14881 cgccaatcgt caagaaatta aagaagctat caagagtggt tatggtgaga gcacgaggtg
    14941 gtcagcgaca cttactgaga agtctatcta tgctgcgcaa cgtttaaata atggaacgat
    15001 agttcgttta tcagttgcgc aacaaactat tttttatctt ctccttggta tgatgagtcc
    15061 cttagcaatt atcatcttac ttgctattat tttatctgta ttaattgcac gatatatagc
    15121 aaaaaaagta tcagagcctt taaacaatat tgacttagat catcctttat caaatgatag
    15181 ttacgaggag ataacaccat tattaaggcg tcttgatagc catcaggcta agattcaaca
    15241 tcaaaaactg ttattacaaa agcgtcaaaa agaatttgat accattattt ctaaaattaa
    15301 ggaaggaatg attcttttag atgatcaagc aagaatcgtt agtatcaacg cagaagcttt
    15361 aaagttattc caaattaatg atgattggca tgggcgcttt atgatggaag ttagccgaga
    15421 tttaactctc aaagacttaa ttgatcaagg actaaaaggg aaaaagaaag aggctaatat
    15481 tgacattgaa aataatcatt accgcgtctt ggtaagacca actacagata acaaccgagt
    15541 aacaggattg gttgttctac tgtttgatgt gactgaccaa ctccaaatgg aacaacttca
    15601 acgtgaattt actgccaatg tctctcatga attgaaaacc ccgcttcatg ttatttctgg
    15661 ttactcagaa ctgttagcta atcaaatggt tccaaacgaa gaagtcccac aatttgcagc
    15721 taaaattcat aaagaatctg aacgattagt taaattggtt gaggatatta tcaacctttc
    15781 tcaccttgat gaacaagaaa aattaccgca agagacagta aatctgtatg acttaacaca
    15841 aaaagtttta gaaggtttac aagctaaagc agataagaag cacatacaaa tcaattttaa
    15901 tggagaagaa gctattttgc gagggaatcc agttttattg aattccttgg tgtacaactt
    15961 gtgtgacaat gctatcacct ataatcatga gaaaggtcaa gtaaatgtga ctttgaaaaa
    16021 ttcacctgat acaattacct tagaagttag tgatacggga ctaggcattg cagagaagga
    16081 caaaaaacgt attttcgaac gtttctatcg cgtcgataaa agtcgctcaa aaatagttgg
    16141 agggactggt ttaggtttgt ctattgtaaa gagtgcttta gattttcaca atggtagtat
    16201 aaaagtggac agccatttgg ggcagggaac aacaatgacc gttttacttc acaaacaata
    16261 aaaactcaca aataaaagtc tagatgatat tatctagact tttttagtaa ttcagaaaaa
    16321 atatataagt aagggtttac aaaaaacaaa taagtgctat aataaaacca taacgcaaac
    16381 gtttgcacta ttaagtgcat aaaaggagat tgaagatgaa aaataatgtc aaacagctct
    16441 ttagttttga attttggcag aagtttggga aggctttgat ggttgttatt gctgttatgc
    16501 cagcagcagg attgatggtt agtattggta actccattag gctattggat ccatcaaatg
    16561 ttttattagg gcgcatagct aatgttattg ctcaaattgg ttggggagtt attggtaact
    16621 tacacatctt atttgcattg gctattggtg gtagctgggc taaagaacgt gccggtggtg
    16681 cttttgcggc tggcttatcc tttattctta ttaaccttat tacagggaac ttttttggtg
    16741 tgaaaacaga tatgttagca aacagtaaag caactgttca aacggtattt ggtgcgacca
    16801 tcagagtttc agattacttt gttaacgttc ttggacaacc tgctcttaat atgggtgtat
    16861 ttgtcggtat tatttcaggt ttcgttggtg caactgcctt taataaatat tataactatc
    16921 gtaaattacc agatgcttta acttttttca atggaaaacg ttttgttcca tttgttgtta
    16981 tttatcgttc agtcattgtt gcattaattt tatcagtatt ttggccggtt gttcaatcag
    17041 gtatcaatgg ctttggtaag tggattgcaa gttcacaaga cagtgctcca atattagcac
    17101 catttgtcta cggtacgtta gagcgtttat tgctcccatt tggtttacac catatgttaa
    17161 ctattccaat gaactacacg caactaggtg gcacatacac tgtccttaca ggtgcaacta
    17221 aaggagcaca ggtgttggga caagatccac tgtggttagc atgggttggt gatttaatca
    17281 acttaaaagg ctcaaattca agtcaatacc atcacttatt gacaagtgtc actccagcac
    17341 gttttaaagt ggggcaaatg attggtgctt caggtatttt gatgggatta tcttatgcta
    17401 tgtatcgtaa cgttgataaa gataaaaaat tgaaatacaa gtcaatgttt atttcagcag
    17461 cagcggctac tttcttgaca ggtgttactg agccaattga gtacatgttc atgtttgcag
    17521 caatgccact ctaccttgta tatgctgtcg ttcaaggatg tgcgtttgct atggcagata
    17581 ttgttaacct ccgcgttcat tcatttggta atatagaatt tttaacacgt gttcccatgg
    17641 gaataaaagc gggccttggt ggtgatattt tcaactttgt ttgggtgaca cttctttttg
    17701 cagtattgat gtactttatt gcaaacttta tgattaaaaa attcaattta gcaacagcag
    17761 gacgtaatgg taattatgat aacgaagagg ttgataatgc tccttcaaca gcttctggtt
    17821 ctgctgatgc aaattctcaa gttgtacaag ttattaattt acttggtggt cgtgataaca
    17881 ttgaagatgt agatgcatgt atgactcgtc ttcgtgtcac agttaaagat gggaatagcg
    17941 tgggttctga agcagcttgg aaaaaagctg gtgcaatggg attggtgtta aaaggtaatg
    18001 gcgttcaagc tatttacgga cctaaagcag atgtccttaa atcagacatt caagatttgc
    18061 tagattcagg tacagtgatt ccaatagttg atttagaaac aggccagcct gtagcagcaa
    18121 cccaagttac aacttataaa ggaataacag aagaaattgt ttcggtggct aatggtcaag
    18181 tagaagcatt ggatgttgtt aaagaccctg tcttttctca aaaaatgatg ggtgatggct
    18241 ttgctgtgga accaactgat ggcaatatct atgttccagt ttcaggtaca gttactagtg
    18301 tctttccaac aaaacatgct tttggtttat taacagaaag tggcctagaa gttttagtac
    18361 atatcggtct agatacagta gctcttgatg gtcaaccatt tgaggttaaa atttctagtg
    18421 gtcaaaaagt ggtagcaggc gatcttgctg tagtagcaga tttggaagct atcaaagcag
    18481 ctggtaaaga gacaagtgtg attatcgtct ttacaaatgt ttcagatata aaaactgtta
    18541 aacttgaaaa atcaggtcca caaatagcta aaacagttgt ggcaaaagtc gaactgtaac
    18601 aaacgatagg agctgggcaa gtctcagctt ctttctgatg aaaggatact ctatggcaaa
    18661 atgcttaacg ttaaatactc attcatggat ggaagtaaat gctctaaaga aattatttga
    18721 tttagcagag catatcttcc gtgaaaaata tgatatcatc tgcctacaag aggtgaacca
    18781 gagcatatct tcccctcttg ctacgagtag tccaaattat catccgattg aaggtacacc
    18841 agctcttcat caagataact ttgccttgca attagtgcat tacctaaatc ttcaaggtct
    18901 acactatcat tggacatggg cttataatca tattggttat agtaagtacc atgaaggggt
    18961 agcgatactt tctctgaaac cattaaagcc agatgatatt ttagtttcag ctgtagacga
    19021 tgagactgat tatcatactc gtcgtgctct ggtagctgag accactctga atgacaaagt
    19081 cgttacagta gttagcctcc atttatcatg gtttgagaaa ggatttgcgg aggagtggaa
    19141 acgactagaa actaccctac ttgaggtaga aactccactt ttactcatgg gtgactttaa
    19201 taatcctact ggaaatcagg gttatgaact tgttcttaat agtccattag cattaaagga
    19261 tagccaccaa atagccaatc atgtatttgg agaccatact attatggctg atattgatgg
    19321 ttgggaaggg aataaaaagg ctcttaaagt tgaccatatt tttactagcg aagacctttc
    19381 aatcagttca agtcaagtag tttttgaagg tggtgaagca cctgtcgtta gtgatcatta
    19441 cggcttggaa attacgttaa atctttagat taatctattt aatatattaa aagcatccta
    19501 aatttgtcta taaaacaaaa cttagggtgc tttttgaaaa atatgtcaca ttatttaact
    19561 tcttacgaag tttggaatgc atgagaaaat atgataaaat gataataact attataggtg
    19621 attttaataa ataatttaat caattggttt atctattaga acaattaata cgaggagacg
    19681 agagtgaata tttttatttt agaggataat tttattcaac ag
//
LOCUS       DATRSG010000036        19713 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRSG010000036 DATRSG010000000
VERSION     DATRSG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19713
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.036"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            100..2601
                     /locus_tag="VBD89_001261"
     CDS             100..2601
                     /locus_tag="VBD89_001261"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO7135790.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            complement(2691..3299)
                     /locus_tag="VBD89_001262"
     CDS             complement(2691..3299)
                     /locus_tag="VBD89_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO7135791.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVAAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTTLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(3392..4780)
                     /locus_tag="VBD89_001263"
     CDS             complement(3392..4780)
                     /locus_tag="VBD89_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7135792.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFITIKKKTYYVMTKDYSGILVDGSIKKAPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(4777..5462)
                     /locus_tag="VBD89_001264"
                     /pseudo
     CDS             complement(4777..5462)
                     /locus_tag="VBD89_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5695..10411)
                     /locus_tag="VBD89_001265"
                     /pseudo
     CDS             complement(5695..10411)
                     /locus_tag="VBD89_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            10734..11426
                     /locus_tag="VBD89_001266"
     CDS             10734..11426
                     /locus_tag="VBD89_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO7135793.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLSTFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            11416..11739
                     /locus_tag="VBD89_001267"
     CDS             11416..11739
                     /locus_tag="VBD89_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO7135794.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
     gene            12109..14346
                     /gene="metE"
                     /locus_tag="VBD89_001268"
     CDS             12109..14346
                     /gene="metE"
                     /locus_tag="VBD89_001268"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO7135795.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKPVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENVAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     RLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRENLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDIKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRSLDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            14391..16235
                     /locus_tag="VBD89_001269"
     CDS             14391..16235
                     /locus_tag="VBD89_001269"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO7135796.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLVTGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNAGQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLR
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNHFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            16582..17289
                     /locus_tag="VBD89_001270"
     CDS             16582..17289
                     /locus_tag="VBD89_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO7135797.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(17454..18539)
                     /locus_tag="VBD89_001271"
     CDS             complement(17454..18539)
                     /locus_tag="VBD89_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7135798.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(18740..19279)
                     /locus_tag="VBD89_001272"
     CDS             complement(18740..19279)
                     /locus_tag="VBD89_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO7135799.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKVLKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         5938 a         3343 c         3775 g         6657 t
ORIGIN      
        1 tggggagaga acctgctgtt gagggtattt tcaccttttt tattgctgtt tttcgtgtta
       61 taataagagg actatacttt tttgacatgg agaagtgcga tgacattcta taatcacaaa
      121 gaaatcgaac ccaaatggca agctttttgg gcagataatc acacctttaa aacaggaaca
      181 gacgcatcaa aacctaagtt ttatgcccta gatatgttcc catacccatc tggagctggt
      241 ctccacgttg gtcacccaga aggctataca gcaacggata ttctcagccg tttcaaacgt
      301 gcccaaggtc acaatgtcct ccacccaatg ggttgggatg cttttggact tcctgctgag
      361 cagtacgcta tggatactgg taatgaccca gctgagttta cagctgaaaa catcgctaac
      421 ttcaaacgcc agattaatgc tcttggtttc tcatacgact gggatcgtga agttaacaca
      481 actgacccta actactacaa gtggacacag tggatcttta ccaagcttta tgaaaaaggt
      541 ctagcttacg aagctgaagt tccagttaac tgggttgaag aattgggaac agctatcgcc
      601 aacgaagagg ttcttccaga tggtacatca gaacgtggtg gctacccagt tgtcagaaaa
      661 ccaatgcgcc aatggatgct caaaatcact gcctatgcag agcgcttgct agaagacttg
      721 gaagaagttg actggccaga atcaatcaaa gacatgcaac gcaactggat tggtaaatca
      781 actggtgcta atgtaacctt caaggttaag gatacagata aggacttcac tgtctttaca
      841 acacgtcctg acaccctttt tggtgcaact tacgcagttc ttgctcctga gcatgccttg
      901 gttgatgcta ttacaacagc tgaccaagca gaagccgttg ccgaatacaa acgtcaagca
      961 agtctcaaat cagaccttgc tcgtacagac cttgccaaag aaaaaacagg tgtctggaca
     1021 ggtgcctatg ccattaaccc agttaatggt aaagaaattc cagtttggat tgcggactat
     1081 gtccttgcca gctacggtac aggtgctatc atggctgttc cagcccacga tgaacgtgac
     1141 tgggaattcg caaaacaatt taagctagac atcattccag ttcttgaagg tggtaatgtt
     1201 gaagaggctg cctttacaga agacggtctt cacatcaact cagacttcct agatggtctc
     1261 gataaagcgt cagctattgc caaaatggtc gaatggcttg aagcagaagg tgttggtaat
     1321 gagaaagtca cttaccgtct ccgcgactgg ctcttcagcc gtcaacgtta ctggggtgag
     1381 ccaatcccaa tcatccactg ggaagatggt acatcaacag cggttcctga gagtgaattg
     1441 ccattggttc ttccagtaac taaggatatc cgtccttcag gtactggtga gagcccactt
     1501 gctaacttga cagactggtt ggaagtgact cgtgaagatg gtgttaaagg tcgtcgtgag
     1561 actaacacca tgccacaatg ggcaggttca agctggtact atctccgtta catcgatcca
     1621 cacaatactg aaaaattggc tgacgaggaa ctcctcaaac aatggttgcc agttgatatc
     1681 tatgtcggtg gtgcagagca cgccgtgctt caccttctct acgctcgctt ctggcacaag
     1741 gttctttacg atctcggtgt cgtacctacc aaagagccat tccaaaaact ctttaaccaa
     1801 gggatgattt tgggaacaag ctaccgcgat agccgtggtg ctcttgtggc aactgataag
     1861 gttgaaaaac gtgatggtag cttcttccat gtggaaactg gggaagagct tgaacaagca
     1921 ccagctaaga tgtctaaatc acttaagaac gttgtcaacc cagatgatgt tgttgaacag
     1981 tacggtgctg acacccttcg tgtttacgag atgttcatgg gaccacttga cgcttcaatc
     2041 gcttggtctg aagaaggtct tgaaggctca cgtaagttct tggatcgtgt ttaccgtttg
     2101 atcacgacta aagagattac tgaagaaaat agcggagccc ttgacaaggt ttacaacgaa
     2161 accgttaagg cagttactga gcaagttgac caaatgaaat tcaacacagc tatcgctcaa
     2221 ctcatggtct ttgtcaatgc agccaacaag gaagacaaac tctttagcga ctatgccaaa
     2281 ggctttgttc aattgattgc accattcgca ccacacttgg gtgaggaact ctggcaagtc
     2341 ttgacagcct caggtgagtc aatctcttac gtaccatggc caagttatga cgaaagcaaa
     2401 ttggttgaaa acgaaattga aatcgttgtc caaatcaaag gtaaggtcaa agctaaactt
     2461 gtagttgcta aagacctttc acgcgaagaa ttacaagaag ttgcccttgc taacgaaaaa
     2521 gtccaagccg aaatcgcagg caaagacatc atcaaagtca tcgcagtacc aaacaaactg
     2581 gttaatattg tggtgaaata agataaagat tttactagaa atagtgatat ttacgcgtat
     2641 aaaatcccaa aagtttatga acttttggga tttttctatt tttagctaaa tcagtcagct
     2701 cgtactgttt ttttagcata gtaagttttt aatacctctt ttgtaatacc atgtttttga
     2761 tagatttttt ctatttttga cgttaatatt tttagttctt tattttggtt ttcaatagag
     2821 ttatcaaatt tttgatagcg gtcgttcaac cttgtctcaa ggtaagcacg ttgttcaaaa
     2881 tataatttta atctatcttt ttcgttagtg gatagggttg tcttatcaat caattttttg
     2941 acttcacttg tgctatccca tgtttgatta tcaatagaag tatagaattt agttagtaga
     3001 cgtttatttt gcttctctag ttggttgata tctttgttaa tatacaattc aataactttt
     3061 aaatcttttg cttttttgac ttcaatatgt cgttggatat ggtcgatttt agcgtagaga
     3121 ggagcagctt ctttatctgc aacttttaac ttatgttcaa tagttttttg actcatatga
     3181 acagttgatg ttttttgtga ttgttcagcc gctacatgat ttggttgtaa actcgtatat
     3241 gttgctaaag agagaacggc aataagcgat aaggaatgta tttttttaga atgtaacata
     3301 taaaaactca ctttctttta gaataacttt ttgatctata ggtattatag gttaaaaaag
     3361 caataacttc tttgaatttt tgtaggaaaa gttaggcaat tttgtggcaa ttgaaataaa
     3421 tcgtaaaagt tgttgaggtc gaatcactct cagcgatgat attaccatga tgttgcttga
     3481 taatttcttt agtcattgca agtccaatac ctgtttggcc gccatgtcct ttatagaagc
     3541 gatcgaaaat ataaggcaag tcttcttttg aaatggctgg tccgtcgttt gagatagtta
     3601 gtttttgatt tgtgtaagag atttcaatat gagaaacagc atagcgtagt gcgttactaa
     3661 ggatattatt gatagctcgt tgaatcaact cttcgttacc ttttagtaac ttttcatgct
     3721 tgtctggtct ataagtgata ctaatctttt gtttattagc taggggtttg aaataagcaa
     3781 taatactatt aagtaattca tttaaagaaa attcatcttt ttgatcactt aagttactat
     3841 ctaatttaga gagtaagata atatcgtcta cgagctgttt catttttttg ctctcttgca
     3901 gaataacaga gtgtgcgagc tcggcatcaa taataccctc ttgaactccc tcagtatatc
     3961 cttggatgga cattaaaggt gtccgtaact cgtgggaggc attttgaaag aagtttcttt
     4021 ggctctcttc tgcctggtag attcgatttg ccatttcttg gctctcaagt agaagaggat
     4081 cgaaatcaga aaagacaatt tctttttgac tagattgatg attgccttga tttttccaaa
     4141 ggctggaaat ataagtttgt actgattgaa tagattctct aattccagtg aaagtcttcc
     4201 tcataataaa tagcatggga atataggtta gtactaagat aactataaga aaatgattaa
     4261 ttttagtaat tagatgctgg gtgtaagtaa tgtctgaaaa gttaatcaca tgaaaaagtt
     4321 gcgactgtgc tttaggagct ttttttatgc taccatcaac aaggatacca gagtagtctt
     4381 tggtcattac ataataggtt tttttcttga tggttataaa cataccatct ttttccttgg
     4441 aaatttgcca caatcttttt tgggtaagat agcgaataat atcatggctc tcttttttat
     4501 gattatcagt gccatatttc atacgataat gctggtcaaa aatagcatag taagaccaaa
     4561 caaagatgct atcttggtta accatatttt catctggtat tttcttgttg ttatagatac
     4621 tatctaaaat ggtgaaattc gcctgcatgg aattccttgt catttgatgg acatagtctt
     4681 taatgacata gttgacagaa aaatagatga ggtaaaaacc aagaaataga gtgatgctat
     4741 taataaggat aagattgcta attaaacttt tctttttcat ttgtcctctt tgattaattt
     4801 gaagccgtaa ccccagacag ttttgatact aacctgactg tggtattgct taagtttttt
     4861 acgtaatcgt ttaatagtgt catcaactgc tctgctttca atatcatcaa atccccaaat
     4921 acgattaagc agtgtttccc tagaaaaagc agtttcaggt ttttcaatta atactaaaag
     4981 aaaatcaaat tctgtcttgg ttaagggcat ttcttcattt gataataggg ctattcgctt
     5041 ttcagtatcc agaattaaat ctagaactga tactgagtgt catttttgat tgctttttca
     5101 tcaaggcgtt tgaacaatgc tttaacatgt agactaagtt ttataggtga aaaaggcttg
     5161 gtgaagtaat cgtcagttcc taaattaaaa gccgtaatga aatcaacatc agaatctcta
     5221 gctgttaaca tgataatagg aatattatca tattggcgga tgcttttcat aacatctaaa
     5281 ccattagttc caggcatcat tatgtctaat ataactaaat cgggtttttg gtttttatag
     5341 gcttctaaga gaaggtctcc agtttcaaat gctcgaacag tgaaaccatc atgttctaaa
     5401 aaaggaacca ccaagtcacg aatattcttt tcatcatcag cgaaataaat tatttttgtc
     5461 atgtatacct caatacccat agttataatt tgacttttag ctagctgttt atgttatatc
     5521 aaatataaca aaaaaagagt ttttttccta ttctttgaag tgttatagtg ttataaacca
     5581 agatattgca gagaaaaggt ctttaggaac atcattttaa gctcctcaaa aaggcaccag
     5641 tcactaacta ttaatagcta gtaattgatg ccttgctcta ttttttaaag tattttattc
     5701 tcttttgata gactttagtt tgattgttgc cagacctagc atagagagac taaatagcaa
     5761 tattgtatag gttattttag gagtgctttc gcctgtttta ggaagcttgt ttgtctttat
     5821 cttttgatta tcggtttggc tagtcacccc taagacacgc ttctgagcaa cctcactttc
     5881 tttagtcatg ccttttttga atatcaacgc ctggggctga tttggcggat tgataggtgt
     5941 ggtattgaga gagtcattat cattgagtga ttgactataa ttctctgcgg aagaaactct
     6001 atcgcgacca tcaagttctt gttcggttgc ttttatttgg tttaataact cttcaacttg
     6061 agcttgtgta gcttgtctat tggaaagcag caattctgct ttcttgataa ggttattata
     6121 attttcttta gcaaattgac tggcgttctg atatttagat gatgtttgtt tcagaacatt
     6181 tgctttaatt atcatatggt gtaaaccaga gtaatcagtt tcatgaccat ttaattgatt
     6241 tttagcaata tcaagatttt ctaaggcttt gtctacttct gattgagtga cgttaggttt
     6301 ttgtagaaga tttttcgcca attggacttg attgatataa gttgattggc ttaatggact
     6361 agcattaaca aattgatagt ttcccttgat ttctgtttca gctaaaatag cttttgccag
     6421 taaagaagta tctgtttctt taccgttaag agcttggcga ctttctctca aagcagcaat
     6481 agcattatca atacttgttt gttccactct atttttaatt aaagtttgtg ccttttcaag
     6541 attagctagg taattatttt ttaagtgagt tccagcatta taaaacacag gttgggaaat
     6601 aatgtcagtt tgttctgcta gggcattaat tagaggagct ttattaatca gcgtcaattt
     6661 tgttagattt acttgacctt cttttacgga tacaagtaaa tcatctaagt tctctaaagt
     6721 gctataccca ctaggcagcg ttacgtttaa acgatagtct cctacaggta tgtttttacc
     6781 atagtcagta ggagaataag ttgcttttgg tagatcaaca acagtatttg tcttagtaac
     6841 caactggact gttgctcctt ttggtaaaag cttgtcaaat tcaacgagta aggctgcttt
     6901 cttgagtaag ttaactttaa ataagacgtc tttaaggcta tctttttcag aaatagttac
     6961 agtgacactt tggggactga ttagatttgc tcgttcctca tcgtataaga ataggtcaaa
     7021 cgtataggta ccaaaaggca atttcagtaa attgaggtct ttcccgtgat gttgcttttt
     7081 gatgacatta cctttgtcat ctttgacaga gtaagagaaa tcaatatcga cattactgtt
     7141 aagctcaggg ctaaacactt tgacgtttac taaaccattt ttattgccaa tattgatcaa
     7201 atcttcgagt tttgctgaca ctagattgcc tgcataatct tcaacagtgt agtagacatg
     7261 agagaaatca ttaacctctt taggtagaat aaagctacca tccttttctt gtttgataaa
     7321 tactttgtta tcttcaacaa ggataccgtc ttctcctttt tgacgtaaga cgctattata
     7381 atgaccatct ttatcttttt ttaagtaaaa aacttgttct ctaaagatac cagattcccc
     7441 gtgttctact gcaggacgag ctttgaagat acgtctatct ttgtcataag ttgctgttgt
     7501 tagtgtagga gcttgtctat ccaaagtgat atcaaatacc atttgttgtg gcttagagcc
     7561 agggacatca gagtagtatg ttaaaacgta tttgtatttt ccatcttcta aaggaatacc
     7621 atcagtagtg gttcctttcc attctgtgtc atataaaaat gtagattttg gattttcagt
     7681 atttccgcta tagtaatttt tatctcctgc ttggggagca cttacccata gtggtttttg
     7741 taaattaaca tcatctgcac gatagacttt ggctcttaaa ttattgaaat tacgtaagaa
     7801 aacacctttc agtgcaacgg catcttgatt ttggtcatca ttaggtgaaa tggctagatg
     7861 aggctggcct ttttgctcta gttgtaagat ccattttcca tctatagact tatagcttcc
     7921 caaggctttc aattggatag gactagtacc gtctgttgag tagataggct ctgaacttgt
     7981 agtcattaag gcagtataat cttctgaacc aggaacttcg tttgtttgtt tcggttggaa
     8041 ataaaatccc ttttctttgt tagcaacaag cttataaatg gatttttcta aaacttctaa
     8101 gttttggaat tctcccttaa atccaacata aggaatacta agaacttccc caccatcaac
     8161 agggtctgta aaacgtacgt agccttctag gaaatagccg tttggcatta ctttttttaa
     8221 catgtcatgg tatttcgaaa catctatatc aattgtaatc gttttagttt gccctggttc
     8281 aatccgtatc gttttccctg taaatgtacc aagttgttga ggagccaatg tgacaaagcc
     8341 atctttaact tgatcagtat ttaaataggt tgtatagtgt aaatcttttg caactttatt
     8401 gatattatga acagtaacat caaaggaaat tttatcttta atatttccta atgtaacgct
     8461 accatagttg ttttccccac cagttaggta taatcctgtc tgaactgctg ctgcgacatc
     8521 gataatacct gctccttgtt ggcgtggtga ggtgtaagcg cctgtatctt tgtttaggtg
     8581 tgctttagca gtactcataa gaaggtattt gacagttctt tcaatgtcac cttttttaag
     8641 ttctggatgt tctttcaata agtattgttt aactagagca gtagcaccag cgacatgggg
     8701 agaagccata cttgtcccac tcatcatatc atattcatta tcattgatag cggcgtaaat
     8761 agagcctcca ggagcagata agtcaggttt taattgtccg tctgctgtta atccccagct
     8821 tgaaaagtca gataggacac ctgcattagg gttggcttgt ttatttttga tattgttaaa
     8881 tacaatttta tagttgtttt tagctaaaac atctccaaac tctttttgag taaaaatggc
     8941 aggaatagcg cttgcttcag gatctaaact cattgttaga ttagcttctc cagctttgtt
     9001 attaaagata atagctccca cagcaccgtg attaatagcg ttgacgactt tttttgtaaa
     9061 agtaatatct ccacgttcaa ttaaggcgat tttaccattt aaagttttgt ccttataatc
     9121 attgtcgttt ccttttccta cgaaaacata atcatattgc ttccctactt caaaggtctt
     9181 atcactaact ttaggatctg cataagcagc taatccgttg ttgagatttt tgttgttttc
     9241 taatccgata atgttgaaga ctttattaac cagtgttgta ttattatatg atgctacaga
     9301 aatggcctca cgagctgttg atggactacc aactaaacca taatcaggat aaagagcaga
     9361 aggctttgat gctccactcc caaatgtacc gtcgttacct gctgctataa caacagaaac
     9421 gccagcgagt ctagccatct ctaaagcttt tataagtcgg tcatcggcat taactaaaga
     9481 gccattagct ccacctaaac ttaaattaat gctatcagca ccgagtttaa cggcatcttc
     9541 aatagcttta acataaaggg ctggtccagt tcctcttttt tcatcagaga agactctcat
     9601 aaacattact tgtgcttcag gagcaacacc atagattaat tcatttgtat ctttcttgct
     9661 aggattagct gttgcgatac tggttacgtg cataccatga gaagtgcttt tcacctcttt
     9721 tagctctgta ttgacatcaa catagttgtg accaaagatt actttgttgt tataccattt
     9781 tccatagttt ataccagctt ttgctttagc agcattcatt tgctgttcgt tttggtattt
     9841 tgcttttgtc gaatcattta attggagagc atcgtgattg gtatctagtc ctgagtcaat
     9901 aattgctaca acagttccct ctcctttgta gcctgtgttc catactttgg ggacagtgat
     9961 aattttatta ctatcaatgt tttgaggggt ttctttagaa atagctggtg taggtttgac
    10021 tgctaaattt tgtttggtta aaggagtttt agaaacttct tgagtgcttt caaccggttg
    10081 atttgcggct ggggaaaatg tctcggtggc ttgagagtga ggctcagtta ctggttgtgt
    10141 ttctgttgga ctattattat tagcctttgt aacaagattg tcttgagctg ttgacgcaag
    10201 ggctgatgac gtgttgttag taggtgtctc atttgctatg ctcgttggac tgtcttttac
    10261 ttgctgttca actgcaatat cagatggctt atttatgaca gaatcagcag ctacatttgt
    10321 gataccgcct aaaaaaaaat agggttccca aaagtacaga tacggcacct aacttatatt
    10381 tccggattga aaaacgctgt tttgtgttca taaaaatctc cttttcttgt aacataataa
    10441 ttacaaaaac tattaaaaca taaaaaatca aaattttcta caattttctg aattttcatt
    10501 acaaattatt ttgtggtcaa tttaagaccg tacctaaagc attttttaaa aatcattcta
    10561 tgaaaataat aatattgtcc aactatatgg ctacaatatc aaaggtttat agaataatta
    10621 caggatattt attagtctaa ttgctggcaa agctaaataa tcattcctct tctttcttag
    10681 taatcaccgt tgtgctataa taactgaata tactgaaaat ttagggggat atgatggaaa
    10741 aaggatttaa agagggagtg aaagatgcct tgcccacagc tttagggtat atctcaatag
    10801 gcttggcgtt tgggatagtt gcttcagcat cagatttatc tgcaatagaa gttggtctta
    10861 tgagtgcctt ggtatatggg ggatctgctc agtttgctat gtgtgcgcta ttattagcaa
    10921 aagcagactt gatgactatt acgatgacag tatttttagt taatttgaga aatatgttaa
    10981 tgagtttaca cgcgactact atcttcaagt ctgctcacct aatgaatcaa ttagccatag
    11041 gaactctaat aacagatgag agctacggcg ttttacttgg agaagcattg catcacaaag
    11101 tcgtatcgcc tagttggatg catggtaata atgtgatgag ttatttgaca tgggtaatct
    11161 caacgataat aggaacttta ctggggagta ctattcctaa tccagaaatg tttggtttag
    11221 actttgcatt agtcgccatg tttataggtc tctttgtatt tcagttgttt gggatgttat
    11281 ctgatggaaa gcgacttgtt gtatatgttc ttgcttctgt aggtttaagt tacttcttat
    11341 tatccacttt tttgtcagga gccttatcag tattgcttgc gactgttgtt ggatgtagtg
    11401 taggagttgt tttagatgat aaatagtacc gtttttcttg ctattctttt aggttttgtt
    11461 gtttcttggg tgccaagagt cgctccattc atatttgtaa aatacaaagg gctaccaaat
    11521 agtcttgtga aatttttaaa atttcttcct ctttctattt tatttgccct aacattctct
    11581 agcttttttt cagttaaatt aggacaattg ccaacactaa aatggttaga tttattggct
    11641 ggcataccaa ctctagttgt cactattcga tatcgaaatt tattttacac tgttcttact
    11701 ggtatcgtta ctatggcttt attgagatta ctattttaac gtgtaggaat ggaagattaa
    11761 taaaaacaca aaaaacgcat ctatttctag atacgttttt tgtgtttttg tctagacagg
    11821 aacttttgat agccgatgtg taatagtttg actaattgtt aataatatca acagattttt
    11881 ctgtatcttg ataatatgag ttgattgtaa gattgctttt taaagatatc tacatgctgc
    11941 tattttaaaa tttataaaaa ggtccatagt ggtttatata gtttaaaact atataaattg
    12001 ctaaaaatat aaaatattaa aagctattga tattatagtt ttcatggact aaaataattc
    12061 ataaaactaa aatggttagt tattagctat aacttgatgg aggaaattat ggtaaaagtt
    12121 tcaaatttag ggtatccacg tcttggtgaa cagcgcgaat ggaagcaagc gatcgaagct
    12181 ttctgggcag ggaatcttga acaaaaagat ttagaaaaac aactaaaaca attacgtatc
    12241 aatcatttaa agaaacaaaa agaggcaggt attgacctta ttccagtggg ggatttttct
    12301 tgttatgatc atgttttgga tttgtcattt caattcaatg taatcccaaa gcgtttcgat
    12361 gagtatgaga ggaatttaga cctttatttt gctattgcaa gaggtgacaa agataatgtc
    12421 gcatcatcta tgaaaaagtg gtttaatacc aactaccact acatagtccc agaatgggag
    12481 gttgagacta aacctcactt gcagaataat tacttacttg atctttatct agaagctagg
    12541 gaagtagttg gtgataaagc aaagccggtt atcactggtc caataaccta tgtttcctta
    12601 tcatcaggaa ttgtcgactt tgaagcgact gttcagcggt tattaccact ttataagccg
    12661 gtctttcaag atctgataga tgcaggcgcc acctatattc agattgatga gccgatattt
    12721 gtaactgatg aaggtgaact tttagtagat atagctaagt ctgtctatga tttttttgca
    12781 agagaagtac cacaagccca cttcatcttt caaacctact ttgaatcagc agtctgttta
    12841 gataaactct ctaagctgcc agtaacggga tttggccttg attttataca tggtagggcg
    12901 gaaaatgtag ctgctgttaa gcaaggtcta ttccgcgaaa aagaattatt tgcaggaatt
    12961 gttaatggtc gaaatatctg ggcagtaaat ttagaagaaa cgttggcttt attggaagag
    13021 ataggtccct ttgttaaacg tttgaccctt caaccttctt caagtctttt acatgtaccg
    13081 gtgacgacta aatacgaaac acatttagac cctgtgttaa agaatggctt atcatttgct
    13141 gatgaaaaac taaaggaatt agaactatta gcgagtgctt ttgatggtaa taaaacaaag
    13201 ggatatcacg aagctttatc tcgtttttca gctcttcaag ctgctgattt tcgtcatgta
    13261 gcgttggaat cattagcaga agtaaggctt gaacgaagtc cgtataaatt acgccaagct
    13321 ttgcaagctg aaaaattaca gttaccgatt ttaccaacaa caactattgg gtcctttcct
    13381 caatcacctg aaattaggaa gaaacgcctt gcttggaaaa gagaaaatct atctgactca
    13441 gattataaag atttcataaa aactgaaatt agaagatgga ttgctattca agaagatctt
    13501 gatcttgatg tgttagtaca tggcgaattt gagcgcgtgg atatggttga attttttggt
    13561 caaaagttag ctggttttac gacaaccaaa ttaggctggg tacagtctta tggttcaagg
    13621 gcggtcaaac cacctatcat ttatggtgat atcaaacata ttcaaccctt aagccttgaa
    13681 gaaacggttt atgcccaaag cttgactaag aaacctgtta aaggcatgtt gacaggtcct
    13741 attactataa cgaactggtc atttgagcga gatgatatca gccgatctga tctttttaat
    13801 caaattgctt tggctataaa agatgagatt caacttttgg aacaatcagg tattgctatt
    13861 atacaagtgg atgaagcagc ccttcgagaa ggcttaccct tacgccagca aaagcaacag
    13921 gcttacttag atgatgctgt tgcggccttt aaaattgcaa cttcatctgt gaaagatgag
    13981 acacaaattc atacacatat gtgttattca aaatttgacg aaattattga ttctatccgt
    14041 tcactagatg cagatgttat ttctattgaa acgagtagaa gtcatgggga catcattgaa
    14101 agttttgaaa cagcagttta tcctctagga attggtctgg gtgtttatga tattcattct
    14161 cctcgcatac ctactaagga agaaattatt gtcaatattc aacgatcact aaaatgtcta
    14221 tcaaaagagc aattttgggt aaaccctgac tgtggcttaa aaacacgtcg tgaagcagaa
    14281 acaattgctg ccttggaggt tcttgtttca gctaccaaag aggttcgtca gcaattagat
    14341 aattaatagg ttaatcagta aaggattgcc atgagggagg agtaaagaag atgtccaagt
    14401 ttttagagaa attaaaaaca gatattctag tagctgacgg tgcaatggga actctccttt
    14461 atacttatgg gctagatact tgtcatgaat cctacaatgt aacacatcca gaaaaggttc
    14521 tagccattca tcaagcttat attgaagcgg gtgcagatgt tattcagacg aatacctatg
    14581 gggctcaacg tcatcgatta aagaactacg gtttggaaga tcaggttgtc tctatcaatc
    14641 aagcagcagt taatatagct catcaagcga ctttgggtaa agaaacattt attttaggta
    14701 ctgttggagg ttttcgttct caaagacaat gcgatttaac attggataat attgtagaag
    14761 aaactttgga acaagttgag gctttattgg tgacaggaca gctagacgga ttattgtttg
    14821 aaacttatta tgatattgag gagataacga cagttttaaa aattgtgagg gagatgactg
    14881 atctaccaat tattactaat atttctcttc atgaagcagg ggtaacatca aatggaaagc
    14941 ctattgtaga ggcgctcagc caattagtta tgctaggtgc agatgtgatt ggcttaaatt
    15001 gtcaccttgg accctatcat atgatccaat cattgaagca agttccttta tttgcccagt
    15061 cttatttgtc agtttatcct aatgcaggtc agttatcttt agatggtgag aatagtcaat
    15121 accagtttag tcagaattca gagtattttg gaaaatctgc agaacttctc gtagcagaag
    15181 gagttaggct tattggtggt tgttgtggga caactccaga tcatattaga gctgtgaaac
    15241 gatctattag aggattgaaa cctattgaac gaaaggttgt tactcctata atacctgtaa
    15301 aagattttgt taggcgtatc agacgaacag atactctagt ggataaggta aaaaaagaag
    15361 taacaattat cgcagaatta gatcctccaa aacatttaga tatcgttcag tttcaaaagg
    15421 ctatacgagc gattgaccaa aaaggtattg cagcaattac tttagcagat aattcactat
    15481 ctaatacacg tatttgtaat ttaagtatag ctagcttgct taaagacgaa attagtacac
    15541 cctttttact acatatagct tgtcgtgacc ataatttaat tggtttacag tctagactgt
    15601 tgggcatgga attattaggt tttaaccata ttttagccat aacaggagat ccgactaaac
    15661 ttggtgattt cccaggagcg actagtgttt atgatgtgac ttcctttaaa ctcctttctc
    15721 tgattaaaca acttaatcaa ggtttatctt atagtggtgc tagcttgaga cgtccaacag
    15781 attttacagt tgctgctgcc tttaatccta atgtgaaaaa ccttactcga accgtaaaat
    15841 tgattgagaa aaaagtagct tctggtgctg attattttat gacacaacca atatttgatc
    15901 actctgtgtt aagggaatta gctgatctta ctaaaacagt tgaacaaccc ttttttatcg
    15961 gaattatgcc aataacaagt tataataacg ctgtttttct acacaatgaa gttcctggta
    16021 ttaaactttc tgagtcattt ttatctgctc ttgaaaaggt aaaggacgac aaagaagctt
    16081 gtttaacttt ggccttaaac gagagtaagt ccttgattga tgaagccttg aaccatttta
    16141 atggtatcta tttgattact ccctttttac gctatgatct gactcttgag ttaattgact
    16201 atatacaaaa aaagcaagta agaaagtcct cttaactttc ttacttttta gttggtcgtt
    16261 tattattata ataaaagtta cgtaccaaaa cagaggttaa actagatttt taagccactt
    16321 tctatactaa gataaattag ttaatttaag ttttctttaa gtttatagac ttagaataaa
    16381 gcgttatttt gatgggtagc ataaaatcaa ggcataatat agatgtcagc gattttttta
    16441 gactaacgaa ataacgatgt aaagcttaat aggggtgtat cgaatactca cttatatagt
    16501 accaaaagcc tatagcctgt tggctagctt gaccgtattc aaatgttgaa taaatcttgg
    16561 ttgagaaagg gaggtataaa aatggtatgt gtcgatttta ttgatcaaaa agtttatagt
    16621 gaagcagaag gtctatttgt ttccgattta gacttaggca ttaaaggttt aataagagca
    16681 cgttatcctt tcgctctgga tagtgacttt atttcgtatg ggaatttaag tcattattgt
    16741 atgaattatc ttgatgaaat tgttcagcga gcgaatcaga aaaatgagaa gataaaatat
    16801 aatgtgacta ccttgatgaa agaagaagag aagcccttca acgttgaaga gcgtttgaac
    16861 aagcaagcaa ctatcggtca acgaattgct gatgatgttg cacgttttgg cggatcttgg
    16921 acttttatta tcgtttttgt atccattatg gctatttgga tgttggttaa tatcatgaag
    16981 ccttttggta ttcaatttga tccttaccca tttatcctgt taaacttagc tttatcaacg
    17041 attgctgcga tccaagcacc acttattatg atgagtcaaa atcgtgctgc tgattatgat
    17101 cgtttgcaag ctcgaaatga ctttaatgtc aataaaacct ctgagttaga aattagatta
    17161 ttgcacgaaa aaattgacca tatggtacaa caagatcagt ttgaattgtt agagattcaa
    17221 aagttacaga cagaaatgct agtatcattg ggaaatcagt tagcacaatt gaaacaacta
    17281 caaaaataat cttttagcag ttatttaaaa atactgatac aatactaaga atttaaatta
    17341 atactgcaac ctcctataat ggagagcagt atttttactt tattaataac aaaaacccct
    17401 tagatgatgt gtctaaggga tttttgtatc ggaataatcg agttggtgaa ttattaggct
    17461 agtgtctgtc cgaaagcgtc tgctgctaaa atagcttgag caaacatttt gggatcgaaa
    17521 ggggtatttt tgtattcatt agtagtcatt gcaatttcaa caatttttgg aatatctttt
    17581 tctgataaag agacgtcagc tagagttgtt ggcaaaccta gtgacttatt aaaacgagct
    17641 actttttcaa gttcttctag ttcgttgaaa taagcatgta aaactaacac accaaacgca
    17701 actacaacgc catgtaagaa ttcaccttgt ctttgaatag cggttgtacc atagtaaaat
    17761 gcatgcgcgt gacaagaatt gtagtagaaa tcaggttgat taactaaatt agaagcatag
    17821 ccagttgaga ttaagatatc aagcgcaatt tcttcaactg cacgcgaagc taaattagct
    17881 tcaacgtctt ttagaccttg ttcaccaaat tgataaaaag cttcctttga agacagagcg
    17941 actgcttgtc ctaacactgc agtatgtggt agtttattgg tcttagcctc taaggtagca
    18001 cgttctactt caggggcttt tgaaataccg tccccaatac cagcccaaaa atatttgctt
    18061 ggtgcctgtg caatgatccg tgtattgata aaaatatgaa gtggagactc gggatagccg
    18121 tatcttaaga aagaatggtc atcattatat acaactgcaa tggcagtacc tgcggagcaa
    18181 ttagaacaaa tagttgggat agtaaagcta tttttaccta actcttttgc taccatttta
    18241 gctgtgtcta aagcttttcc accgccaaaa cctaagatag catcagcagc ttgaacttgt
    18301 gggttggcta ctaacttgtc aatgttagat tgtgttgaat ccgttccata gacaaagctt
    18361 cctgtgactt taatatttgc ttcctctaaa atagagcgaa cctcggcttc tgagctgtct
    18421 agggcacgtt tgccacccac gaaagcaatg ctttgaatac ggtaagttgc caaaacgcta
    18481 ggaatttctt tgaagcaatc ttctccgtag ctataatttg ccattacatt tgttgtcatt
    18541 cagtgtcctc ctctattcca aaggtcgatt agagacagct attttctaaa agtgaatagt
    18601 tttttgtctc ttgaccaagt gattatcata ttatagcaga atattcagaa aattggaaca
    18661 tgaaaaataa tttaattttc aagaataaga atagaaaagg taaagcgaga tatacgagta
    18721 aatactgtca gtataataat tataagctct tgagagggtc tgtattggat gcaacaggga
    18781 gttcttttag gtattgatca agatcgcgtt ggtgttttag cacctttatt ttttgagggt
    18841 agtcctcaac aacttgttta tagtttgatt tttgtttgtc agagcgaccg tctttaagaa
    18901 tccaactgat aaattcaaaa tctaattttt ccgggcatcc attggccata tcaggtctag
    18961 tcttaccacg aaacttacag taacgtttga atgctctcca caagcagttg aagcgattaa
    19021 agtcaaaaaa aataatctca tctgcatctg ctagacgttc ttgatatagc agttttttgt
    19081 agttaccttc tataatccat gttctttttt ctaacatgct gctgacatcg tcaatcatgt
    19141 catcatattt tctctcttcc caatttgggg cgaagtgaat actatcaagg tgtaatacat
    19201 tgcaattata gtaatttccc aattttctcg ctaaagtgga cttacctgac ccactatatc
    19261 cgattattgc tattttcata tactacctcc gttaaattgt ttacttgaag ttaatgctgt
    19321 acttaggatt tacttatttt ataagggtta ttcacttttt tagattaata tatttttgat
    19381 ataagttatc catacacatg gtaacataat taccagtaaa tgtaatctag atatcaatac
    19441 tatggttgac gatattgtta acttttgggt tcaaaaaatt ggggtaaaaa tcatcggagg
    19501 gaactgccaa acaaactcac agtttaaaat actaagtaat aaaaagtctt gaatttatta
    19561 tgtttaaggc tttttaagcg tctaaaatac ttagtctagt atgaatttct aaacagaaag
    19621 taaaaataat gagttttagt atggtttttg ggcataatgg aacgatttta tctaaaatag
    19681 tacgtttctt attaaattta tttttttaga aaa
//
LOCUS       DATRSG010000037        19637 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRSG010000037 DATRSG010000000
VERSION     DATRSG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19637
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.037"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..497
                     /locus_tag="VBD89_001273"
     CDS             <1..497
                     /locus_tag="VBD89_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135800.1"
                     /translation="NISKEENRHFELLLKAKLNELLYLLYYHQYVIKKHTDDTYRKNE
                     RIRDLIDYINNNYQQNLTIEFLADYMGYSKTHFMTVFKQHTGTSCTEFIIQVRLNKAS
                     EHLINSTTAIIDIANSVGFNNLSNFNRQFKRYYHTTPRQYRKQFTNKKDGSTLVYRNI
                     NTKK"
     gene            complement(554..2086)
                     /locus_tag="VBD89_001274"
     CDS             complement(554..2086)
                     /locus_tag="VBD89_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO7135801.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2195..3268)
                     /locus_tag="VBD89_001275"
     CDS             complement(2195..3268)
                     /locus_tag="VBD89_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7135802.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3261..4037)
                     /locus_tag="VBD89_001276"
     CDS             complement(3261..4037)
                     /locus_tag="VBD89_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135803.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(4034..4828)
                     /locus_tag="VBD89_001277"
     CDS             complement(4034..4828)
                     /locus_tag="VBD89_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135804.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(4812..5966)
                     /locus_tag="VBD89_001278"
     CDS             complement(4812..5966)
                     /locus_tag="VBD89_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135805.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(6015..6917)
                     /gene="murB"
                     /locus_tag="VBD89_001279"
     CDS             complement(6015..6917)
                     /gene="murB"
                     /locus_tag="VBD89_001279"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO7135806.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(7061..7549)
                     /gene="folK"
                     /locus_tag="VBD89_001280"
     CDS             complement(7061..7549)
                     /gene="folK"
                     /locus_tag="VBD89_001280"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO7135807.1"
                     /translation="MTTVYLSLGSNISDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(7546..7908)
                     /gene="folB"
                     /locus_tag="VBD89_001281"
     CDS             complement(7546..7908)
                     /gene="folB"
                     /locus_tag="VBD89_001281"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO7135808.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(7910..8713)
                     /gene="folP"
                     /locus_tag="VBD89_001282"
     CDS             complement(7910..8713)
                     /gene="folP"
                     /locus_tag="VBD89_001282"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO7135809.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTGPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(8717..9280)
                     /gene="folE"
                     /locus_tag="VBD89_001283"
     CDS             complement(8717..9280)
                     /gene="folE"
                     /locus_tag="VBD89_001283"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO7135810.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(9299..10561)
                     /locus_tag="VBD89_001284"
     CDS             complement(9299..10561)
                     /locus_tag="VBD89_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO7135811.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQIPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(10563..11450)
                     /gene="rarD"
                     /locus_tag="VBD89_001285"
     CDS             complement(10563..11450)
                     /gene="rarD"
                     /locus_tag="VBD89_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO7135812.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(11437..12303)
                     /locus_tag="VBD89_001286"
     CDS             complement(11437..12303)
                     /locus_tag="VBD89_001286"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO7135813.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(12305..13588)
                     /locus_tag="VBD89_001287"
     CDS             complement(12305..13588)
                     /locus_tag="VBD89_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO7135814.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            13765..14653
                     /locus_tag="VBD89_001288"
                     /pseudo
     CDS             13765..14653
                     /locus_tag="VBD89_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(14676..16223)
                     /locus_tag="VBD89_001289"
                     /pseudo
     CDS             complement(14676..16223)
                     /locus_tag="VBD89_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(16366..17742)
                     /locus_tag="VBD89_001290"
     CDS             complement(16366..17742)
                     /locus_tag="VBD89_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO7135815.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(17816..18823)
                     /locus_tag="VBD89_001291"
     CDS             complement(17816..18823)
                     /locus_tag="VBD89_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO7135816.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(18931..19617)
                     /locus_tag="VBD89_001292"
     CDS             complement(18931..19617)
                     /locus_tag="VBD89_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135817.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         6664 a         3957 c         2939 g         6077 t
ORIGIN      
        1 ttaatatttc aaaagaagaa aatcgacatt ttgagctact cctaaaagct aaattaaacg
       61 aactcttgta tttactctat tatcatcaat atgtcattaa aaaacatact gatgatacct
      121 acagaaaaaa cgagagaatt cgtgatctga ttgattatat taataataat taccagcaaa
      181 atcttaccat tgaattttta gctgattata tggggtatag taagacacat tttatgaccg
      241 tatttaaaca gcatactgga acatcctgta cagaatttat catacaagtt cgtcttaata
      301 aagctagtga gcatcttatt aactccacta cggccattat tgatattgcc aacagcgttg
      361 gttttaataa tctatcaaat tttaatcgcc aatttaaacg ctattatcat acaactcctc
      421 gccagtaccg caaacaattt accaacaaaa aagacggtag tacgcttgtc tatagaaata
      481 taaatacaaa aaaatagaaa ctagttaaat ccatgactag tttctatttt ttattattta
      541 agcgttaatt agtttacatc agctgctcac gtacttcttt aatttccgat tcattcacaa
      601 ctaaaaaaat ggtgtcgcca gcattcaaga tagtatttcc tgatactaca gccgatgtct
      661 tatgatgaat ttgagtcgtt attagcacat tttctggtaa ttctaaatct cggacatact
      721 ttcctgatat tttatctgaa acagttaatt caatcaatgt aggtgtcatt actttatctt
      781 ttggattgaa ggtcatttta gccaacatcg cttcataaat aggttctccc tttaataaat
      841 ccatgacaat ataagatacc attgttacaa ttccaatagc cattaattgt tttaaatcgc
      901 caaccatttc tgtaaccaaa atcatagctg ttagtggtgc ttttgagatt gcaccaaaat
      961 agcccgccat tcctagaaca acaaataaag atatctgctg atgagatatt ataccaaagt
     1021 actgcatacc tactgcaaaa aaagcacctg ctaaagatcc taaagctaga attggtaaaa
     1081 aaattcctcc tggcaagcca ctactgtaac tcaacatact ccataaaaaa cgaatgagga
     1141 aaaataatcc taacatcatc agactaagat tactcctagg cagtgataca attaagccat
     1201 ttccaccccc caataattgt gggaaatagt aaccaatagg aagaataaaa attaccgcta
     1261 atataccata taggtgagat ggtagatgaa atagtttacc taaataatca tagatcacat
     1321 gaaatctcaa aattacccac tcatagataa agcctaagat acccaaaaag agacccatga
     1381 gtaaaaaaat ccaataaaag ttgagattta aagatggtaa ttctgatggt aaagctaaaa
     1441 ctggggttaa accaaagata ttaagtgaga caaagtttgc cactaaactt gctaccaaag
     1501 ctgttatcca aactaagcga gaaaaatgat ggtaaatttc ttcaactaca aataaaaggc
     1561 ctgcaatagg agcattgaag gctgctgaaa gacctgctgc tgcaccacta gcaattaaaa
     1621 cacgtttttc cattcgacta gcattgagat actgtgcaat tcccttacct gtcatagctc
     1681 ctaactgaat actgggacct tcgcgaccaa gcataagtcc cgaagaaata gctaaaatac
     1741 ccgcaatgaa tttcttccat acaatactaa accagtctgg cgatagcatt cctttaagtt
     1801 caccttcaac atgcgggata cctgaacctt taatatcagg atcagatttg ataaaaaaac
     1861 ctattatcac tgcagcgata atactagtaa ccgtaatgct caataaaata attggttgat
     1921 aatgggcact tttataaagt tccataacaa ctaaaaaaat tttttcaatc gctaatcgaa
     1981 atatgctgac aacaaatcct gctattaagc ctactactat tcctctccaa acaaatccca
     2041 gaattgattc aagtgaaaac tgaaattcat ttttatgatt ttccatattt tcctcctttt
     2101 cttacaatta ttttaacaaa aatatcacaa actttctagg aactcttggt aactaaaaat
     2161 acctctcatc agagaagtac ttttagttaa tagattactt acgatacatt ttaaattgta
     2221 agtacaaatc attataaata ccaagccatt tttgacccaa gttattataa acttctaaat
     2281 gatcaattgt tttatcagga ggataaaatg ctttatcatt tttgatatct gctggtaaca
     2341 gcgcttttgc tttgagatta ggggtcgcat aaccaatata ttctgcattt tgtgccgcat
     2401 ttttaggttc catcatgaaa ttaataaagg cataagcttc tttgcgatgc tttactgttt
     2461 tggggattac aatattatca aaccaaaggt tcgatccttc actaggaaca acgtaatgca
     2521 aatgcttgtt cccatcaagc atttctcgtg cttctccaga gaatgtaaca ccaatcgctg
     2581 catctccctg aatcatatag cctttcatct catcagcaac aatagcctta acgtttggtg
     2641 ttagagcatc taacttttta gaagcggctt ttaattctga tatgttcttt gtattcaatc
     2701 catatcccaa ggaatttaaa ccaactccaa taacctcacg tgcactatca actagcatga
     2761 ttttatttcg aaactctgga cgccataaat catcccaatg tttaggagga gttttaacta
     2821 attgatcatt ataaacaatc ccaacagttc cccaaaaata aggaatcgaa tatttatttt
     2881 taggatcgaa actcaaattt ttaaacctag ctccaatagc atcccaatta gctatctttg
     2941 aatgatcaag tttaaccaga aggttttctt ttatcatttt gtcaatcata taatcactag
     3001 gtacagcaat atcatatgtc gtcccaccct gcttaatttt ggtgtgcata gcctcattag
     3061 agtcaaacgt ttcgtattgt acttcaattc cagtttcttt tgtaaatttt tttaataagg
     3121 ctggatcaat atagtctccc cagttataaa taactaactt atctgagtta ggtgttgaac
     3181 tactcttctt ttctaagtaa gtggttaaac caaagaggat caggataaca agaataatac
     3241 cgcctaaaaa ggaataaact ctacgcattc ttttctccct tctctttcga aatatagtaa
     3301 taaccaatta ccaataaaat actaaataaa aagacaatag ttgacaaggc attaatttcc
     3361 aatgatatac cgcgacgtgc gcgtgaatag atttctactg aaagcgttga aaagccatta
     3421 cctgtaacaa agaaagtaac cgcaaaatca tctagcgagt aagtgaaagc cataaagaaa
     3481 cctgaaataa tacccgacga caaatagggt aacataactt ctttaagcat ttgccaagtt
     3541 gaagctccta aatcatagga agcattaatc atatcatcat tcatttcttt taaacgaggt
     3601 aagaccatta aaacaacaat aggaatagag aatgcaacat ggctcaataa tacagaagtg
     3661 aaacctaact gtaaaccaat aacagtaaat actaagagaa aacttgcacc aatcataaca
     3721 tctggtgcta ctaaaaggat attattaagc gataaaatag aggtttgatg tctacgtctt
     3781 acctgataaa tccaaatagc tccaaaggta ccaataattg tagctaataa ggcgctaaga
     3841 aaagccaaaa agaatgtttg cactaaaatt agcattaagc ggctatcctg aaacagttct
     3901 ccatagtgac taaaggtgaa tccagtaaaa gaattcatat ccccaccttt attgaaagaa
     3961 taaaaaatca aataaataat aggaatataa aggataataa aaacaaatgc taaatagata
     4021 tttgcgaatt ttttcatgct ttcctctcct ttgttaacca cataatagca accatcacta
     4081 aaattaaaat aacccctatg gttgagccca tacctttatt ttgtgtgatt aaaaaatgtt
     4141 gttctatggc tgtccctaaa gtaataactc gattaccacc aataagacgc gtaagcataa
     4201 agagacttaa actaggaata aagacagatt gtacacctgc tcttacacca ttcagagata
     4261 gaggaaatat aaccttctga aaagtttgcc atgcatttgc acccaagtca tcacttgcat
     4321 aaatgagatt ttgatcaata tcatccaagg cattaaaaat gggtaataac ataaacggta
     4381 attcaatgta agctgctaca aaaagaaatg aaaaatctgt aaatagaatc tgttttgggc
     4441 cgatacctat aaaggttaag aaggcgttaa ttcccccttg ttgaccaaat atccccataa
     4501 aagcataggc ttttaacaaa agattaatcc atgttggcaa aataattagc attagccata
     4561 attgcttgtg ttttaatttt gtaagcaaat aagcagctgg atagctaatc aaaagcgtta
     4621 tgaaactaac gatgcctgca tataggaccg agttaaagct cattttaaga taggttcctg
     4681 agctaaaaaa cgtctggtaa ttagctaaag taaagtgccc attaatatca aagaacgagt
     4741 tccaagcaat caaagcaaca ggtgctaata caaataagaa taaccaagcc atataaggaa
     4801 ttgaaaatag actagaggtt ttcttcattt ctctcctcct cgatagcatt gattaaacca
     4861 tcttcatgat cgtcagtgtc tacatattct tcaatgcgcg catcaaattc ttcttctgtt
     4921 tcattaagac gcataatatg tatatcttct ggagtgaaat ctaacccaat aacttcaccc
     4981 tcaatagctt tacgagttga atgaatcatc cattcattac ctaaatcatc ataagcaata
     5041 atttcatagt ggacacctcg aaaaagttgt gtgtccactt taacttgaag ttttccttca
     5101 tcgggtaggg taatttgaag atcttctgga cgaataacta cttggactga ttcattaggg
     5161 cgcataccgc catcaacagc ctcaaagcgc ttaccgttaa attcaaccaa ataatcctca
     5221 atcatcttgc cagataagat atttgattca cctataaaag ttgctacaaa atgattgata
     5281 ggttcatcgt agatatctac tggtgttcct gattgaacaa tttcaccttc attcatgacg
     5341 aaaatccaat cactcattgc caacgcttct tcttgatcat gtgtgacaaa aacaaaggta
     5401 attcctaaac gttgttgcag ttcgcgcaat tcatattgca tctctgtacg caatttcaaa
     5461 tccaatgctg ataagggttc atctagtaag actactttag gttgattgat gatagcacgc
     5521 gctatcgcaa cacgctggcg ttgtcctcct gataattttt gaatagctcg cttttcaaac
     5581 ccttctaaac gaaccatttt tagagtttct tgaacacgtt tttgaatttc tttcttatcc
     5641 atttttttca actttaatgg aaaagcaacg ttttcaaaaa ccgtcatatg tggaaacaaa
     5701 gcatagtttt ggaaaacggt atgaacatcc cgtttattag tgggaacatc attgatgcgt
     5761 ttcccatcta agtaaatatc gcctgtactc gcctctagca aacctgcaat aatatttaag
     5821 atagttgatt taccacttcc tgaggcccct aataaggtgt aaaatttacc ttcttcaagt
     5881 tcaaaattaa tatcttttaa gacaaccgtg ttgctgtctt cgaatacctt tgatacattt
     5941 ttaaatgcaa tgattgggtt agtcaattct cataaatcct ccatttttct gactaaagta
     6001 agtactaaac tactttataa tagcgactca ccgataatac gaacttctgg ttccaaatgg
     6061 acacctgaag ttgattcaac ggtgttaata acatgttcaa taagattttc ataatcttga
     6121 gctgaacctt ctgcaatgtt aaccataaaa ccagcatgtt tttgactaac ctctactcca
     6181 ccaatacgtt gacctttaag atgtgcttcg ctaatgagtt gccctgcaaa atgtcctgga
     6241 ggacgtttga acacagaacc acaagaaggg tattctaaag gttgtttcag ctcacggaga
     6301 taggttaaac gatccatttc ctgagtaatc atgagatgat ctcctggttt taaggcaaac
     6361 ttagctgata taacaatatc tccactctct tgaatgacag aatgacgata accaaattgc
     6421 atattcctag cttcaatcgt tttgagttca ccctgtggtg tcaaaacttg tgcagacaat
     6481 aaaatatgag caatttcacc accgtaagcg ccagcattca taaagactgc tccacctaca
     6541 cttccaggaa ttccgcaagc aaactcaaag ccagttaaac tatgataacg tgcaatacga
     6601 gttgtttcaa tgagattagc tcctgcttct gcttcaataa cataaccgtt gactgtaaca
     6661 gttgacagtt tatcaaacat gataacaaaa ccacggatac caccatctct aacgataata
     6721 ttactcgcat ttcctaaaac catccaagga atattttgac tgttagcaaa cttaacaata
     6781 cgcgatagtt caaggcgatt ccttgggaat gcaagataat cagcaggtcc tcctacttta
     6841 gtataagtat atttctttaa aggctcatca aagcgaatat cgagaccttc taattctttt
     6901 tgtattgttt taatcataac ctttatctca attctgtttt attgtgtttt atagacacaa
     6961 aaaatcggca taaaaccgat ttctattata ccaaaaatat aggttgttga cgatacatta
     7021 tcacaatttc tgtgtatttt aattttaaaa tcctaactga ctattcgact ggttttaaat
     7081 aatgaacttc tcctaaattc aatttttcga gatagtcttc tagcttagaa ccattaggta
     7141 aacttagttg aggtgctatt tcgagtaacg gaactagaac aaatgctcgc ttcgtcatat
     7201 acggatgggg tactactaaa tcttcttggt taatcacttg attaccaaaa agtaagatat
     7261 caatatctat tgtccttgga ccccagtgct catgtctcac gcgctttaag cgtttttcaa
     7321 tttcttgaca ataatccaat aattcaaaag gtgctaagtc tgtctctacc tgacaacata
     7381 tattaaaaaa atcttcttgg ttagtattcc cccaagcagc tgtttcataa atagcggaaa
     7441 tttgcgcgac ttttgtcttt tgcaaatgat caattgaaaa gagagcctgc ttcagaaaag
     7501 tttctctatc actaatatta ctacctaaac tcaagtaaac ggttgtcata ctcgcttgcg
     7561 ctccaattca attccaactg attcatagtg cccattaatt ggtggattgt cttttgtaat
     7621 ctttaatgta atagcttgta ctggatgaaa ctgtaagaaa atatcttcaa caataacacc
     7681 tgctaatttc tctattaatt gatactgttc gttttcaaca tgatttttaa tacagtcaaa
     7741 aacttcacca taatgaaccg tatcaattaa atcatcagtt tgtgatgctt ttgctaaatc
     7801 aagtgataga acagcatcaa cttgaaaaac ttgccccaat gtttgttctt ccgaaaaagc
     7861 accatgataa ccataaaacc gacatttatt taaataaatc ttatccatat tacaccaact
     7921 gactaattac tgtcacaatg tccttattta cttcaacatt gtgaacacgt acaatctgac
     7981 acccctttgc tattgcccat gccgataaag cagcagtccc catatcacgt tcagttactt
     8041 ccctattacc acctagaaga taattaacag tacgttttcg agagatccca aataacacag
     8101 gataacctaa atcacacact tgttccaaac cttttaatag ctctaaattt tgctcctctg
     8161 ttttagcaaa accaaagcca ggatctatcc aaatattatc ctttgaaaca cctgcttcta
     8221 aagcacgctc tgctctttca agtaagaatt cacaaacttc ttttttgata tcttgataaa
     8281 cagcttcttc ctggttatgc atcaaaataa ttggaacatt tcgttctgct gctaaagata
     8341 gcattttacc atcatataaa cctgaccaaa cgtcatttaa aatatgtaca cctgcatcaa
     8401 gggctgcttg agctacctct gttttataag tatctattga taacaaaaga tcagggtaga
     8461 cctctctaat agctttaatc attggaacta cacgtttaat ttcttcttca gcagtcacaa
     8521 atactgcacc tggccccgtt gattctcccc caatatctac aattgccacc ccttgttcaa
     8581 gcatctcacc aacttgattt aaagctgaat caattgtggt ataagaacca ccatctgaga
     8641 aggaatcagg cgttacattt aaaatcccca tgatacaagc tttacctgta atatcatatt
     8701 gtcctatttt catcttttat cctttttgta ttagcgatag aatttcttgt ctttcatatc
     8761 gatcgtcctt gtataaacca cgagctaccg ttgtaatcgt cttacttcct ggttttttaa
     8821 tgccacgcat tgtcatgcac atatgctctg cttctatcat aacaaaaata cctttaggcg
     8881 ctaaagcatc ctctaaagct tgtgcaactt gtgctgttaa acgttcctga agttgaggac
     8941 gcttactcgc cacttcaact gcacgtgcaa gcttactaag tccagtaaca cgtccatcgt
     9001 ttggtaaata agcaatatga gctttaccat aaaaaggaac caaatgatgt tcacacatgg
     9061 aatagaatgg aatatccttg acaatcacaa cctcttcatg attttcagaa aagactgcag
     9121 tgaattgctc ctttggatct tgattaagtc ctgagaacat ttctatatac atttttgcca
     9181 cacgcttcgg tgtatctttc aacccttctc gatcaggatt ttctcctaaa gcctctaaaa
     9241 attgataaat agctttttcc attttttctt gattgggcat taatttatcc tctccttttt
     9301 aagttgttgt agttttgatt aatagtagtt cctgacgaac ttgagaaata aaataaaggg
     9361 agccagtaat cacatagaaa tctttatctg atgtcagatt tatatcatgt agccatttct
     9421 tccaatcact aactctagtg tacgctttag gatatttgtc taagtttatt gatttagggt
     9481 aatcaaagct agtaaccgaa accggagcga tactagacaa tgattctaac atactctcta
     9541 tcggtttagt attaattgca gcaaatagaa tatgcacgtt tttctgctga tattttttta
     9601 taacttgaac gagagcatcg acactttcat tattatgagc gccatcaatc attacatttg
     9661 gaaagattaa ttctgtacgg cctacccatt ttgtcatttc aagcccatct ttaatggtct
     9721 gtagggttaa ctttggatat ttgtctctta ataataaact ggtagttata gctatgctag
     9781 cattggaaac ttggtgatgt cccggaattt gcaactgaat atgatcaata tttgcttgag
     9841 gacctatata gttaaaataa ttttcctctt cttttatata aaagtcattg tgtaaacgat
     9901 aagtctggct gtgggtttcc ttggctttta tttgaaagac ttcttcaaca tcctgacgat
     9961 cattagcata tacgaatgga actccttttt ttaaaacacc tactttttgt tcagctatat
    10021 ctacataagt tcgtcctaaa acctcctgat gatctaatcc aattgacgga caagtaaccg
    10081 ctaaagcctt gaacatgttg gtagaatcat aataaccgcc cattccagcc tcaataatga
    10141 cgatatcaac tggacagcta ttgccaaaat aatagaacat caaaactgtt ataacttcaa
    10201 attctgtagc tggttctagg ttggtctcaa ggtgcaaccg ttcaacaact ggacgaacag
    10261 tctcaacaag tttgataaaa tcagactctg gaatcatttg accatcaatg ctaatacgct
    10321 ctctaaagtc aacaatatat ggtgatgtaa atgttccaac ttgataacca gaatttgtaa
    10381 aaatatgttg taaataacta gttgttgatc ctttaccatt cgttccaaca acatgaatgg
    10441 cagataggtt gttttgaggg tttcctaact gctctagcat ccatctcata cgttccaaac
    10501 ctggtttaat accaaaagct aatttgctat gaatccattc tagcgcttct tgataagtca
    10561 tcttatttac ctttctttaa catcgtatgt acttgtccaa tactaaaaac taaaatagca
    10621 agccagataa aaataaaacc tattacctcc ccactaacga tagtttcttt aaaaataaac
    10681 aatgctagca ataattggat ggttggattg atgtattgga taaaaccgat aatatttaga
    10741 ggagccctct taatggcttc tgcaaataat aaaagaggaa ctgctgtaat gattcctgaa
    10801 agtgagagta aaactaattg taatatatta taatcagtta aaaaatcttt ggcgaaaaac
    10861 actatataaa tgagtgcaaa cggagcaata aaactacttt ctaccagcat cgaaaaatcg
    10921 ctagataaag aaattgattt ttttaataac ccataaaaac caaaagaaag cgctagagtt
    10981 agtgaaatta aaggaaaatg accagtttga taaactaaaa ttccaactcc catgattgca
    11041 atcaaaattg caaggctaac tactcttgcc aggtgttcac gtaaaacaag gaccgacaac
    11101 aaaattgaaa taatcggcat catataataa ccaagacttg cctcagtagc gtgaccatgc
    11161 gtgactgcaa aaatatagac caaccaatta attgaaatta aaatactggc tacaaacatt
    11221 gtccaaaatg actttttatt attaactaag ccttttaagt cttttaaata gactgttttt
    11281 ctacccgaaa cgagcatata aataagcata gttaaaacag tgaatataat cctataagca
    11341 aacgtactat atgcttcaat tccagataat aatttccaat aaagagataa aagcccccat
    11401 aaaacatagg ctgacaagcc aaggataatt cctaaattat cttttctcaa ctctcactcc
    11461 ttttcgctca atattaagta cttgaatctg tccatctaaa cataatttac ttaactctgt
    11521 gacaattggt tgttcttttt ctttcgaaca cagaaccatt accgtcggtc ctgctccaga
    11581 caaataagta gcatacgcac cgttttgagt gctgatttgt tttataactc caaattcctt
    11641 caccaaaggt tgacgatatc tttcatgaaa gaggtccctt tctatagccc atccagcagt
    11701 taccaaatca cctttcaaca acgacgcaac catcacatta gcgacgctgc ttgaagcaac
    11761 tgcatcctca taactcaatt tttgaggtag tacatttcga ctatcacttg tttttaattc
    11821 ataatttgga ataaaagcga taaaggaact atctggaaac atcacttcca aacttttaat
    11881 atcatttttc actattgatg aaataactaa atcaccaaaa atggccggtg ctacattatc
    11941 aggatgtcct tctaatcggg tagctatctc taatttttct ttttgagaaa gagccaaatt
    12001 tcctaattga ttggctaatt ctattcctgc aacaattaca gaggaagaag aacctaaacc
    12061 tctggccaaa ggaatatctg aaaacatttt taagcggtgt ggtgccaaat ccgacttaac
    12121 atgcaaagct gtttgaataa ggaggttagt atgatctttt ggaatattta ctaaattgtg
    12181 ttcaactaac cactctgtac tttcttctaa tacttcaatg cttaaatact ttgataacgc
    12241 aactcctatt gaatcaaagc ctggaccaat attcgcacta gttgcaggta caattatcct
    12301 catttcaccc tcctattact ttgaaaacat tgattaactg aatgacacca ctctcaggaa
    12361 actcctcaat aaattgagtt attttctctt ggctaacttc atctgttaat acatagattt
    12421 cttgttgttt atcatccaat gctttttcaa taacattttt caaacctata ttttcttgct
    12481 caaagtacct tccaagtgta tcactatctt cagcatttct caaagaaata gctagatagt
    12541 actcattgaa gatatcagac tgcattgaca acctacacgg acaatgataa gtattcatag
    12601 gcttaatttt tatctgtgat cgaatactac gactaatatc gatgatatcg gctaaaacag
    12661 aggttgctgt tggattttgt cccgcacctg gtccataaaa aagagactga ccaatcccta
    12721 ttgattcaat gaatactgca ttcatcacat gattaactgc agctaaggga tgctcttgag
    12781 aaattagtgt cggagaaacg tctacactta taccagttgg agtttcacga agatcagtta
    12841 ccaacttaat ggcaaagccc atctcctgag ctacttcaac gtcctctgag cgaatactag
    12901 aaatcccctt ataattaaca tcatcaaaat caataggcat accaaatcca aattgactta
    12961 agatagtggc tttgtaggca gtatcaatac cttcaacatc atttgtagga tcactttcgg
    13021 cataacctaa ctcttgtgcc ttttttagag cttgttcata agaccatccc tcttcaaaca
    13081 tttttgttaa catgaagttg gaggtaccgt tgagaattcc taataaacgt gtcattttat
    13141 ctgaggcaaa cgaatgcgat agggtcctta aaataggaat gcctccagca actgctccct
    13201 catagaataa agctagacct ctagcgtctg ctaaatccat caactctttt ccgtgtaaag
    13261 caatgagatc tttattggct gtcacaatat gtttaccaaa tcctaatgct tgactcaaat
    13321 aagttttagc tggctcaatc ccccccatta attccactac aatatcaatt tgtggatctt
    13381 gaagaatagt atttatctct gtcacaaagt tataagtaaa tccctgattg atgaaacgct
    13441 ggcggctttc attatctctt actaatactt tatcaattac aatatcttcg ccttctaaag
    13501 aaagtagctt atgttgattt tcttttagca aatatggaat acccttagca accgttccaa
    13561 aacctaataa agctatttta atagtcatga atttctcctt agaaattgtc tctaacttca
    13621 agacaaacat tggctatatt ataacaaatt accaatattt tttatagggt aaacgcgtct
    13681 tgtcctatga aagttcttaa aaagagaata ttatttcaga aaagatgata caataaaagt
    13741 actttaaaag ttaaggagaa ataaatggca cacacaccta ctagtcatcg taaaccacga
    13801 aaacgttccc catggttagc tattgcttct gtcttttttt tgttaatagc tttaatcggc
    13861 atatttctat tcttcaacaa tcgttcaaag caagaaataa aaacaaaaac gaacgcctct
    13921 agtcatcgaa aaattgtcac atctatcaaa aaaaagaaat gggttaagca aaagactcct
    13981 gttaaaatac ccattttaat gtaccatgct gttcatgtta tggacccttc tgaagccgcc
    14041 agtgctaact taattgtggc acctgacatt tttgaaagtc atataaaacg tctcaaaaag
    14101 gaaggatatt acttcttagc ccctaacgag gcatatcgtg cactaaacga aaatgcttta
    14161 cctgaaaaaa aaagtcattt ggattacttt tgatgatgga aatgccgatt tctacactaa
    14221 ggcataccct atactcaaaa agtacaaggt taaagccact aataatatca tcactggctt
    14281 tgttcaagaa ggacgagaat ctaacctcaa cgttcaacaa atgctagaaa tgaagcaaaa
    14341 cggcatgtca ttccaaggac atacggttac tcaccccaat ctttctctct taactccaga
    14401 actacaaacc caagaaatga cactatcaaa actgttttta gatcaaaaac tctctcaaga
    14461 tactcttgcg attgcttatc catcaggacg ctacaatcct acaactttgg atattgctag
    14521 tcaatattat aaactagggc ttacaacaaa cgaaagagtt gcaacaaaag ataacggttt
    14581 gctttctctt aatcgagttc gtatcttacc aactactagc gatgatgatt tgatcaaaac
    14641 gattaaccaa taaaaagaag ccttctggct tctttttatt cttcataaga tttaaatggt
    14701 tcatttttac gtggataacg atctggagaa atcgataagc ccatttcttt tttctcatgg
    14761 aaaagctcca ctaatagcgc tagcatcatc aaaatcatta cgaatgaaaa tggcagagcg
    14821 acaataatta aaacgttctg cagcgctaac agaccaccag acaagagtaa gacaatagca
    14881 atcactgcca acactagccc ccaaattact ttagttcgat tttttggatt taaattacca
    14941 tcttctgtca acatagccaa aacgtacgtt gcagaatctg ctgatgtaat aaaaaatgaa
    15001 aaaattaaaa tgatagcaat agtggataaa agccaaccta aagtgtagtg attaaaagta
    15061 gcaaacaata cttcctctgt tgcaaacttt gtcaaattaa tacccagttg ttgtacctga
    15121 gttgataatg tgccaaatgc tgcaaaccat acaaaactca ataatgtcgg tattaaaaga
    15181 actaccgtta aaaactcccg aatgctacgc cctttagaaa tacgagcgat gaaaactcca
    15241 acaaaaggag accaggaaat ccaccacgcc caataaaaaa tcgtccaatt atcaatccaa
    15301 gaacgtttag tattgtcaaa agcagctgca cgaaagctca ttccaaagaa attttgtaaa
    15361 taagagccta aagactctgt tagggtatca aaaatacgaa ccgttggtcc caatacaata
    15421 actaaagcta agagggctac cgctaaaatc aaatttaagt tcgataaaat tttaacacct
    15481 tttcctaaac ctgataaagc tgacataaca aataaagctg ttgtaataag gataataaca
    15541 atttgaacaa atgcattatt gggaacaccc aataaaaaac ttaaccctcc gttgatttga
    15601 gcagctccaa atccaagtgt cgtagcaact ccaataactg tagcaacaac tgtggtgata
    15661 tcaacaattt ttcctagtca accatctgtc ttatcaccga aaagaggctt taaagtgaca
    15721 gacaagaggt atttctcttg ctttcgaaat ccaaaataag ctagagccaa agcaaccaat
    15781 gcatatactg cccaagcatg tatcccccag tgaaaaaatg taaaacgaaa tgcatcagct
    15841 aatgctgttt gcgattcctt aggtgcgcca ggtgtcgaaa tagcaaaatg cgataagggg
    15901 tcagctgctc cgtagaagac caaaccaatc cccatacctg ctgaaaacat cattgctatc
    15961 catgatacag ttgaatattc aggacgtgaa tgaggtcgcc ctaaacgtat ttgtccaaga
    16021 ggactcataa taaaaaagac acacaaagca agaattgaag taactaacaa caagtacaac
    16081 caaccgaaat tggtagatac ctgttcacgc aagatttgtg tccaagattg aaaaccacga
    16141 ggaacaataa ttcctaataa gactaaaata agcgatacaa ttagtgaacc tgtaaacaca
    16201 ggcgtaatat gttttttact caaaatttcc tcctatacta aaatagtata gcatacttta
    16261 ataaatactc ctctcatttt aatcacctgt atcaagacct ctccttgtct cctcaacaaa
    16321 aaagaaccag ataacattat ctggttcttg tgtttgactt agtgattaat cactatttgg
    16381 tgtaaatacc aaatgttcgt tgacaaaggt atcaaatcca agttggctca attcacgacc
    16441 atatcctgaa tttttaatac ctccaaacgg taattccggt aatgatgtcc accctgaatt
    16501 aataaatgtc atccctgttt caatctgagc cgccactttt ttagcatgtt ctggatcaga
    16561 agagaaaaca gtacttccta aaccataact agaatcatta gctaaagcaa tagcttcttc
    16621 ttcagtatcc actttataga tagaggctac ggggccgaaa atttcttgat tatagattgg
    16681 atttgcttta gtgatattcg ttaaaacagt tggcatgacg aagtttcctg gatgatctat
    16741 agtgtcattt ccaaaaacta cctcagcacc gtggtctacc gccaatttaa tttgttttaa
    16801 aacatcatcc tttgcacctg cagatgacag tggagccaag gttgtttccg aatccattgg
    16861 gtcaccccat ttagcagact taaaggtctc aactaccata tttacaaatt cgtcatactt
    16921 atcagccata acaatgaaac gttttgaaga tgtacatact tgacctgcat tatataaacg
    16981 cgcaaagaat attgttttac taagtaaatc aaaatcagca tcatctaaaa taagaaaagc
    17041 atcgttccca cctaattcca tagacgattt tttcaaattt ttaccagcct cagctgctat
    17101 tgatgcccca ccacgctcag aaccagttaa acacacacct gcaacacgag gatctgatat
    17161 aaggtttgaa acttgatcat acgaagcaaa aatattttta aacgctcctt ctggagctcc
    17221 tgcttctcgg actaaatcct caaatgcttg agctgatgca ggacaaatag aagcatgttt
    17281 taaaagcata gtattaccca ctataaagtt aggggcaaat acacgcataa tctgataaaa
    17341 tgggaaattc catggctcga cagcaagtaa aacaccgaca gcttgtttca aatagtaagc
    17401 ctctccattt ggactttcta acggaacagg ttttaaaaac ttttgcccat tatcagcata
    17461 atagtcagct atgtctgcac acaagtccac ttctccttgt gcttcagtga aaagttttcc
    17521 catatcttta gtcataactt cagcgtactt atcacggtcc ttacgtaata aatctgctac
    17581 tttatgcaat tgattttggc gttcttcaac attatcttct ttgcgccatg tcttgtagag
    17641 ggcatgcgct atgtctaatg actgttcgag atcactgtca ctgatattat cgaattcatg
    17701 caatacttcg ttagtgtacg gataaattgt tttatatgcc ataatgttcc ctccttttga
    17761 tatcctcatt ataacattta tgaaatcgat tacaaatatt tagctttaga caagatcacc
    17821 aagttccaag gatagcttgc attgttaaac taactataat aatgcctaac caacacgctg
    17881 cacctaaaag aattgatttt cctccagttt taattagttt gctgacatta gtatttatac
    17941 caattgcccc cattgccata acaataaaaa atttagataa aaccttaagt ggctcaaaga
    18001 tacgtaacga aaaacctaat gaagcaacta tcgttgttag caaagaagct aagataaaat
    18061 aaagcacaaa cttcggaaat atcttagtaa cacttacaga ttgttctttc tgcgtttgtt
    18121 tcatatgata aatagataat acaatcgtaa ttggaatgat ggctaatgtt cgtgtcaatt
    18181 taacaatagt agctccgccc aaagtattgc tgtgattgat agcatcccat gcagtagcag
    18241 ttgctgtaac tgacgaggta tcattgacag cagtccctgc aaataatgca aaaccgtgat
    18301 cagagagacc aataaaattc cccagcgttg gaaaaattaa agccgctaag atattgaata
    18361 aaaaaataac cgaaatagct tgtgctactt catcatcttt agcattaata actggtgcag
    18421 tcgctgcaat tgcggatcca ccacaaatag atgaaccaac acctattaaa gtagcaatcg
    18481 ttttatctaa cttaaataac ttttgcaaaa cataagcaat aatcaatgaa atggaaatcg
    18541 tcataataat gatgggtaac gatgatatac caactttcat cacttgcatg aggtttagtc
    18601 caaaacccaa taagacaact gctgtttgta aaatatattt agacgtaaaa gcaataccct
    18661 tattaaacaa ttgtcgattt tgataaaagg atccaacaat tattccaatc aagatagcaa
    18721 agactggtgc tcctattaaa gggagataaa gcccaagcaa ccaagatggt atagcaataa
    18781 taaagcataa tattagtcca ggaatttttt ccttaaataa catgatagtc ctcctcacat
    18841 tgactattat atcacaagct ttaaaacgtg gcaggaaaac cgaaagagga ccagaatatg
    18901 ctctgatcct ctttcattac taattcacaa ttatttagtt actttcataa atggatatga
    18961 tttttcgtta cctaaaatca attttgagcc ctctttttta taggctgttt tttcaccatt
    19021 tttcacaata ttaccataga acgcatttgc ctttttattg atatgcgata ctttaataac
    19081 acttgttaag cgatgtactt taccatccat aactggcaat gtcatttcac cagtattagt
    19141 attatatttt cctccgacag cttgtgcagc actttggaaa cttctatcaa tttgttggtc
    19201 aatttgtttg tcagtgattt ttgtttcatc aaaaatcttt ttacctgctt cacttaaatc
    19261 attgtaagtc aaaccttgat cttttaattg tttttcaaga gctttatcaa taaaagtttt
    19321 tatagcttga tggaatttta cctcatcaat ttgagcagtg actgttatct ttgcctcatc
    19381 gtttttgacg ttcatatgca tattagcgcc cttaaccaaa tcagctgcac taatccccat
    19441 atccgctgcc tgacttccaa gttgttgttc tatttctttc tcaacttgct tctctaaaga
    19501 aactgtacgc catttcccct ctagtgactg acgattattc cagaaataga gaccgcctgc
    19561 acctacaatt agaataagta ctagtacagt taaaaaaact tttaaaaact ttttcatgat
    19621 aaaaactcct tatgata
//
LOCUS       DATRSG010000038        19472 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRSG010000038 DATRSG010000000
VERSION     DATRSG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19472
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.038"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(148..1575)
                     /locus_tag="VBD89_001293"
     CDS             complement(148..1575)
                     /locus_tag="VBD89_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO7135818.1"
                     /translation="MTIFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(1592..3460)
                     /locus_tag="VBD89_001294"
     CDS             complement(1592..3460)
                     /locus_tag="VBD89_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO7135819.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            complement(3453..4304)
                     /locus_tag="VBD89_001295"
     CDS             complement(3453..4304)
                     /locus_tag="VBD89_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO7135820.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            complement(4642..5250)
                     /locus_tag="VBD89_001296"
     CDS             complement(4642..5250)
                     /locus_tag="VBD89_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO7135821.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            complement(5330..6367)
                     /locus_tag="VBD89_001297"
     CDS             complement(5330..6367)
                     /locus_tag="VBD89_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO7135822.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            complement(6421..7149)
                     /locus_tag="VBD89_001298"
     CDS             complement(6421..7149)
                     /locus_tag="VBD89_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7135823.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            7325..8317
                     /locus_tag="VBD89_001299"
     CDS             7325..8317
                     /locus_tag="VBD89_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7135824.1"
                     /translation="MKNYRKLIVLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(8376..9320)
                     /locus_tag="VBD89_001300"
     CDS             complement(8376..9320)
                     /locus_tag="VBD89_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7135825.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(9331..10140)
                     /locus_tag="VBD89_001301"
     CDS             complement(9331..10140)
                     /locus_tag="VBD89_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7135826.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(10266..12503)
                     /locus_tag="VBD89_001302"
     CDS             complement(10266..12503)
                     /locus_tag="VBD89_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO7135827.1"
                     /translation="MLHLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTITIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPFVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDNLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            complement(12487..13140)
                     /locus_tag="VBD89_001303"
     CDS             complement(12487..13140)
                     /locus_tag="VBD89_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO7135828.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTLD"
     gene            complement(13240..13980)
                     /locus_tag="VBD89_001304"
     CDS             complement(13240..13980)
                     /locus_tag="VBD89_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO7135829.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            14115..14879
                     /locus_tag="VBD89_001305"
     CDS             14115..14879
                     /locus_tag="VBD89_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO7135830.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            14872..15138
                     /locus_tag="VBD89_001306"
     CDS             14872..15138
                     /locus_tag="VBD89_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO7135831.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKNEQ"
     gene            complement(15321..16907)
                     /locus_tag="VBD89_001307"
     CDS             complement(15321..16907)
                     /locus_tag="VBD89_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7135832.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(17142..17990)
                     /locus_tag="VBD89_001308"
     CDS             complement(17142..17990)
                     /locus_tag="VBD89_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135833.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQAMSSKQIKDKNSKEK
                     VITVATYSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEF
                     FMSIFNKENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLL
                     LQEKKLITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTK
                     INLVPKRDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPS
                     EADVAF"
     gene            complement(18006..18668)
                     /locus_tag="VBD89_001309"
     CDS             complement(18006..18668)
                     /locus_tag="VBD89_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135834.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            complement(18661..19395)
                     /locus_tag="VBD89_001310"
     CDS             complement(18661..19395)
                     /locus_tag="VBD89_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7135835.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLA
                     LVYKLNHQKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILC
                     DEISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDT
                     VVPVKATLNQLKSNYVDQVKEFLLHD"
BASE COUNT         6471 a         3715 c         2892 g         6394 t
ORIGIN      
        1 caatccaaga aactttaagg atgcaacttt attgtatcta gcacttaaat cagaagatat
       61 ttaccttcat aaattataat aaaaaggagt aacctatgga tactcctttt tatattaaat
      121 tgaaacagag actagttgtt taacatgtta tctgttatga tgttcaaaca atgattggcc
      181 atttgtctta atcacttttt ggtaccaacc aaatgacttc ttcttgtaac gttgtagact
      241 ccctgttcca tcatcatttc gatcaacata tattagacca taacgtttac ttagttgagc
      301 tgtagacatg gatacacaat caatgcatcc ccaagatgta tatcccatta tttcaacacc
      361 atcttcaata gctttagcaa cttgaactaa atgttgattc atataatcaa tacgataatc
      421 atcttcgaca gtataatcac cgtctagtgt ttctatcaat tgatcttttg ctcccaatcc
      481 attttctaca ataaacaacg gaatctgata acgttcataa tactgattta agactaggcg
      541 tagccctatg ggatcgattt gccataccca ttctgaagtt gttaaataag gattagtcag
      601 gcctccaagg atatttccct gaccactttg ataattttca aagtcataag cttgtgtcac
      661 gctcatatag taactaaacg ataggaaatc tactgtattt tgtgccaata cttcctcatc
      721 tccttcttca aatttaattt caataccatt atttttgaaa taggactgaa tataagtggg
      781 atatttacct ctgacatgta tatctgaaaa tagcagatta tgttgttcaa attgtcttgc
      841 agccagaaca tctcttggat cagatgtcat aggataagct ggcatagcca gaatcataca
      901 gccaatttta aaattagggt tgatagaacg ccctaatttt gtcactctag ccgatgctac
      961 taattcatga tgaatagctt gataaagctc ctgaggactt agttgtgatt tgtcggccat
     1021 aatagcacca ctggtgaaag gcatatgtaa aatagaattc acttcattaa aagttaacca
     1081 atattttacc ttatctttat aacgctccat gactgtttga gcaaatttct caaaaaatgc
     1141 tatcaaacgt ctatcagccc aaccattata ggtctttgca agatgaagcg gtgtttcata
     1201 atgagataag gtaaccaaag gctcgatatt atatttaagc aactcgtcaa ataaattatc
     1261 ataaaattgt aagccagctt cgtttggagc actatcatca ccatttggaa aaattcgaga
     1321 ccaggcaata gaggttctaa aaactttaaa gcccatctct gcaaacagtt ttatatcatt
     1381 tttatagtta tgataaaaat caattgcttc tagcttcaaa ttgtctggag taggtttggc
     1441 ggtaaaatcg cctagaccac catttggaag tacatcttgt acagataaac ctttgccatc
     1501 tgttctaaat gcaccctcta cctgattggc agcaactgca cctccccata gaaaatgttt
     1561 aggaaaaatc gtcatattat catccttcct tttaaagaat tacaagtaaa tcatcttcat
     1621 ttgtaaccat tgtctcttcg tctttaacga taatttcaag ttggtcaatt gaatttgtaa
     1681 ctacaactgg actaataaga gaatacccct ttgaactaat aaaatcaata tccattcgca
     1741 aaagtaactg tcctcgttta acaaaatcgc caactttaac caattgttca aaaccttctc
     1801 catttagttc tacagtatct attccaatat ggattaacac ttcaactccc ttatcatcct
     1861 ttaaggcaat ggcatgtttt gtaggaaata aacttataat ttcaccatca aatggtgcat
     1921 aaacttcacc cacactagga gtgattgcta aaccttcacc tagtaattta gatgaaaatg
     1981 tttcatcggg tactttagat aaaggtaata cattgcctac cataggtgaa tacaatgtct
     2041 ctttttttga taaaccactt ctaatatgtg tgtactcttg agcattaata ctactttttt
     2101 cgttttctcc ttcatcaatt cccaagaacc atgtaataat gaaagtcaaa ataatagtag
     2161 ctattgctac aataacagca ttactgaaat tattgccccc ctgtggattg atgtattgtg
     2221 gcaaaccaat aatagatggc actacaaatg tataggaagc aatattaact aatccaatat
     2281 aagctcctac taaaccacct gatatcattg cagcgtataa aggcttcttg aatttcagtg
     2341 tgacaccata aagtgcaggt tctgtgatac cagctagtag tgctgataaa cctgctgcaa
     2401 aggccacctg acgtgtctgt ttttgtttag ccttaactgc aacagcaagg gaagctgcac
     2461 cttgagctaa attagaagct aacattgctg gtaaaatcaa aacatctggt gtcgtaacag
     2521 aagcagctaa aaagattgga gcaaatgccc agtgcattcc tgtcataaca accaatggca
     2581 taatagcacc aagaatagct aaagctaacc atggtgccac atggtaaatc gaaagcattg
     2641 cactagacaa tcctttacca ataattactc ccagtggccc cacgaccact aaggctaaaa
     2701 agccagaaat gagaataatc aaagtcggtt gtaaaaagct cttcattaca ctaggaacaa
     2761 ttttattaat ccatctctca atgtattgca taagaaaaac cataattaag attggaataa
     2821 cagaagaacc ataactagcc agtgtaaccg gagctccaaa gaaagataat ggttttcctt
     2881 cagctaccat tgtcacaaaa gcagggtgta agagaatccc tcctactgta gcagctaata
     2941 caggtgtaac tttaaatcgt gacgcagctg aataagcaat cattattggc ataaaataat
     3001 aagcggcatc accaaaaaaa ttgataaaag ctactgtttg agaactagag cttgttatac
     3061 cgagcatagg gagcaaaata cctataacct tgagcatacc tcctcctaga agagcaggta
     3121 taagcggctt aatgactcct gcaatcgttt caattaattt ctcaaatatt gaactctttt
     3181 tttgtgatga aaattctctg gtagtatttt caaaatgacc taaggccaaa aaagcattat
     3241 aataattatt gacatcattt cccaaaataa tttggtattg atcattttta cgcataacac
     3301 cgataacatt cggaatagtt ttaataacct gatcgttgac aatttcatca ttgtctaaaa
     3361 ctaatcgtaa tcgtgtcaca cagtgggtta cgtgttgaat atttttttct ccccctactg
     3421 ctgctaaaat agctttagca gtctcttggt atttagtcat ctgatatctc ctttaaattt
     3481 ttaactaatc gtttaacatg caccgtcaga tataggagtt cacttgaatt aagttgatat
     3541 tggtattgga tagccatatt gttgccgatc tctttaacac atttatattc tctcggatat
     3601 tttttcttaa ctaccaaaag taaatcagca tcatcatctt cgtatttcat tcctgataaa
     3661 acgcgttgag caaataattt ggtatgtgtc atgaaacgat aataatcaat tgaactttca
     3721 tctagttctg ttcgaaaatc tatttttact ttctgttcaa tgtaagatac aatctcagtg
     3781 attttatgcg cttcattaaa cggattatcc aatccagcat ttacaaagtg catagcaata
     3841 aaagctgact catcaattgt taaacaaata cccaattcat cctttataag ttccagagcc
     3901 ttcatcccaa tggagtattc atcaggatag taacgctgga tttccaacct tagtggattt
     3961 tggataacca ttctttgttc atgtctttca atagccgaat gaatatgatc tgtcaaattg
     4021 atataaagaa tttcatctaa gttcttaccc aatttgattt tacctaggtt gataattcgt
     4081 tctgaacatg caactacttc ttctggaaca gtaataaata actccgtaaa acggttcatg
     4141 ttatcactat tttttaaaac aaatgacttc tcaatagcat cgctattaat cctatcacct
     4201 attcttttct taaatgcaat tcctttcccc atcagtaaca catctaatcc ttgatgtgtt
     4261 actgatatga ctgcattatg atttaatacg cgttttataa tcattaaagt cctagtcctt
     4321 cactcgcaca ataaaaaatg acccatccaa gacacaacaa aaagtcgtcg ttgaataggt
     4381 ccctgctcac aaaatgtgat aacatccttt attatgcaat tttatctgtt atcagcttgc
     4441 ttaccttaaa taataacacc tgaaaacgtc ttaattttat catttcttac taatcttgtc
     4501 aagcgctttc aataaactta ttcgcttaat tgaagataat aggactgttc tagttctatc
     4561 tgagtttgga tttctagact tttaaaaaca ttatcaactt ttagaataat aaaaaaacga
     4621 gcctatcgac tcgttttttt attaagcttt agcagcttgg taaagttcat ttactttatt
     4681 ccagttaata atttcaaaga aagctttgat gtagttagga cgaacattac gatagtttaa
     4741 gtaataagca tgctcccata catcaagtcc taaaataggt ttcttacctt ccataattgg
     4801 agtatcttga ttggcagttg aaagcacttc aagtttgcct tcagcattaa caacaagcca
     4861 agcccaacct gaaccaaaac gtcctgttgc tgctgctgtg aaagcagctt taaagtcttc
     4921 aaatgaacca aaagttgcat caatgtcttc agataactct tgtgaaattt gagtttcttc
     4981 tggtgacatc aattcccaga aaagagcgtg gttaagatgt ccaccaccgt tattgatgac
     5041 tgcctgacga atatcttctg gaatttgaga aacatcagct aagagcgcct ctaagtcttc
     5101 tccaatttca ggatgtttct caagagcagc atttgcatta gcaacataag ttgcatggtg
     5161 cttatcatga tgtagtgtca ttgtctcagc atcaatatgt ggctcaagcg catcatatgc
     5221 atagggtaaa tctggtaaaa taattgccat gtcgaaatcc tcttttcatc tattgttacc
     5281 cttattctat aggaatttgg aacagctttc aactcttttg tttctttaat tagggctttt
     5341 tatgagcaat ttttaataag gctaaatcaa agagataatc cttatcataa gtccctcttt
     5401 taatagcata gtcagtctcg attaaaatcc taatcgcttc ttttaaaaaa gcaaggggaa
     5461 ggtttctaga atctctgaca gcaaatttta cttgataagg gttgatttta cgtcctatgt
     5521 aatgggatag ttcagaaaca atttgactct ctgactttcc tttactagcc aatattttaa
     5581 cttgcaaaaa catccgaaac tgtcctaaca tgattgcaat caatttgatt tcgtcttcgc
     5641 cttgtagacg taaatctctt actaaatcac gcgctaaatc tatcctacca agtaagacat
     5701 cttgagtcaa atcaaatata ttatcttgca ggcttttagg gatagcctct ctaacatcat
     5761 tagacgaaat atgaccgtct gttttatatg actttaagaa agcgatatta gttaaagtat
     5821 cactaaaatc atagttagac ttaatcaata actcatcaaa aacacctgct tcaaaaacta
     5881 atccttcttg gtgagcatat ttttgaaaat acgtctttaa atctgattct tttaaagtat
     5941 tagcctctaa aacacgagca tcacgtttca ataactttac aagtcgacgt tttccatcaa
     6001 gttttcctgg agcacaaata acaagtctcg tagtatctac aggattctgc aagtaggctt
     6061 ccaagcgctt cattgcttgt tcatctaaat aagttttttt atcagttgta atatcttgaa
     6121 actgatcaaa aataacaacc ttgtagtctg ataaaaaagg taaactttct aagtctaatt
     6181 ccgcattttg atattcttct tcacttaaat caaaataaga ataggctaaa tcgtctttat
     6241 tgaatccaat tacctgaaat agcttctctt tcatctgagc atattgccct aaatcttctc
     6301 ctgctaaaac agttactaac cctaaattat ctggggtaat tctcccaatt tcttcaatcg
     6361 caatcatatt ccttattata ccataaggaa acttttcaag gtgtcaaata atacttaaaa
     6421 tcattcatca actctatgag aaataattcc aaattgacaa tctcggataa aatggtcatt
     6481 attctcttct aattcttttt gtaaattgac atcttctaac ccctgaaggt cactatattt
     6541 ttgtattctt tctttcatga tatttagtag ccaagataga tctgtaccag tttgtgacgt
     6601 ttgaatgaca ttttctgata caaaatggtc aacgattaag cctgcttcta acattaattg
     6661 ctcaagttga cttccaatat gaatgtgtcc tccttccttc tcaacagttt gccagattag
     6721 tgattgtatt ttattcatat ttacaaagct aggcttagct agttctaaac acattgcatc
     6781 actttcttga aaaacataaa aaccattttg ctttaacaaa ggtgttaaac tttctattac
     6841 tttctttgga tttttttgat acattaatac acgacgtcca ataatagcat caaaactttt
     6901 tgtttcaaag gaggaaagtg ctgctaaatc tgcattttga taactaacat tagcatagcc
     6961 attatcatgt ctagcttgcc ataaaagttg ctcattaata tcaattccta caacatcacc
     7021 ttctttacca acgatatcag cagccaaacg cgttaattcc ccactaccac aaccaatatc
     7081 cataactctc ataccgggct gcaaagcttt ttttaataat gcttttgaaa aatcttctct
     7141 gtgaaacata ataacactct cctctcttct attttagcaa attaaaggtt catacgtact
     7201 atcagcaaaa aattataata ggttgaaatt ttatagcatt attttcttgt tttcgtatct
     7261 actttattat agaataacaa ctaatcattt ttagcatgac gagctcaatt acaaaaggat
     7321 aatcatgaaa aactatcgaa aacttattgt actactactt ctaatctttt ttgccatttt
     7381 tatgggagca tatgcttaca cgcatattgt tgaaaaaaga tccctaacta gcaatactat
     7441 tgaaaaaact ctacctgtgg taaatcagat taagcctcaa accattaaag aataccaaaa
     7501 ttacttaact aaggtagcta aacgtaatgt tcttcctgta gacattcctc aggcattaaa
     7561 taatgaaaag gtagaaatta ctgctactga tggcatgcaa acattcactt ggaatgataa
     7621 aaataatcct aagcaaaagg ttatcttcta tgttcatgga ggatcatata tccatcaagc
     7681 ttccgaatta caatatattt ttgtcaataa actagctaaa aaattagatg caaaagttgt
     7741 ctttcctatt taccctaaag ctcctacata taattatagt gatgctatcc ccaaaattaa
     7801 aaaattatac caaaatacat tagctagcgt cacatctcac aaacagatta tcctagtagg
     7861 tgaaagtgca ggcggaggcc ttgctttagg tattgctgat aaccttgtca cggagcatat
     7921 caaacaacca aaagaaatta ttttaatatc accatggtta gatattgcta ctaataatcc
     7981 aaagattgaa aaagttcaaa aaaaagatcc ccttcttaaa gcttggcaat tacaacaagt
     8041 agctccttac tgggcaaatg gaaaaaagaa ttttaaaaat cctcaagtta gtcctcttta
     8101 cagtagccaa tttaacaaga tggctccaat atcatttttt ataggaacgc acgatatttt
     8161 ctaccccgat aatcaattat tacatcaaaa attagcaaaa gaaaacatta aacaccacta
     8221 tatcgttggt caaaaaatga accacgttta tccagtctta ccgattcctg aagcagagac
     8281 tgctttcaaa caaatgctta aaattatcaa tcattaaata aaaaggtttc caatcaagtt
     8341 tagttggaaa ccttttttct attacttttg aatgactaat tatcttgaga taaaaactct
     8401 gagaataaaa cttcttgact aatcttttca gctaaagcct gtttaaaagc taatttccct
     8461 aaataatagg atttgtggtc aattgttggg agtttcaaaa gttgcccaac taataaacat
     8521 tcctgaccaa taatataagg acattttttc ctactttctt cataaccttt tattagccct
     8581 gccgcaactt catcacttgt agctaatata ccatctatac taacttcttt taccaattga
     8641 taagataaat ttagtccatc attaaagtca tgaacattcc caactaccat atatggagag
     8701 gatagttgcc catagacctc ttgataagct aataatgcgg attgataggt agcagaggat
     8761 tcattattcc ttgaaaataa taaaactaag tgctccaaac ctcgtaactt catatcagaa
     8821 aatgcctcta gaaatgaaga atagcgatca aggtaagcag aggatagatg attatattcc
     8881 tgcaattttt cacaaactac tattcgtcca tattttgcat aagtttcaat aatgtcgagg
     8941 ctaattgctc ttgaagtgaa tattaaagca tcaatagctt ccatcttgag ctgtttcaag
     9001 taggaaagtt ctaattcttg gttgtaatcc gaaggcatca caacaagttg atagtcggta
     9061 gttttagcag catctaatag gccatttatt aattgtgtaa aataaggatg gcgcgtgtgg
     9121 ggaatgacga cacctatttt ttgtgttttg cctcgactta aatcccgcgc tagttgattt
     9181 ggtatataat ccaattcggt gataagagcc attacttttt gtcgcgtctc atcagaaaca
     9241 tgtgggtgat gattgataac acgggaaacc gttgatttag caacacctgc ctttttagct
     9301 atatcactaa tacttgtcat tataaggctc ctactttaaa aatttttgaa tcccatatcc
     9361 cactccgtct tcatcattag acttagttag ttgatatgct atcgctttga tatcatcaaa
     9421 agcattatcc ataacaatag ggtaacctac catttcaagc ataggcaaat cattatgtcc
     9481 atctccaaat gcagccgttt cttctctagc taactgctct ttttgaagga tgtaagcaat
     9541 tcctttagat tttttggcta agagatgtgt aatttcaaga tacgctttac ctgaacgttg
     9601 aatagtgatt tctggtaact ccaaactttg aagatatttt tctaattcta gcatatttgc
     9661 ttcatcgaaa gtaatcatca taattttaaa agtatttgtg tgaccttcta aaaactgatc
     9721 ctcattatga ataaacgttg ggcattgttg agttaaacta tgttcgtatc ggattccttc
     9781 atctatttta tcacaatacc agttattaag atcgtaataa gataaactaa cttgaggaaa
     9841 atggaaccgt attcctctta gcaattgttt tactgtgctt tttttaatga tttgtgtatg
     9901 aataggtaga acttgattat tatctcctat tctataaatc aatcctccat taaaagcaac
     9961 ttggactcct cctaattgaa gtgcatctac ggcatccttc atttccatag gtgccctagc
    10021 agacactaaa gttactggaa tcgctgcctc acgtattaag gtagcgttac ttttactcac
    10081 ttgtccttca ctatttaata gtgtaccatc catgtcacaa aaaatacgtt tgatagtcat
    10141 aaaaaatacc tctcttcttc tgaaatcagt ataaatgctg gaacccgttc catgtcaagc
    10201 ttaaaattac tgaatccttg cgaaaaatag attgtattta acagatacgc aatcatttta
    10261 ataaatcaac gaactgtctg aatatgccac ttccaccatc ctgttagcct aattgtacct
    10321 gattgatccg ttcggaaaat cttgctttta atcttttgga acctttgtaa agtctcttga
    10381 tgaggatgct ggtaacgatt atttttacca gctgacacta gaaccacact aggagacaac
    10441 tttttcagaa aggatagact tgatgagccc ttagaaccat gatgtcctgc tttaaggata
    10501 tctactgata aattaggata agcttctaat aactgctttt ctccctcttc ctccaaatca
    10561 ccggtaaata agaagccttt tcctaataaa tgtccatata aaacaattga atcattattt
    10621 tttccatcac ccttgtgcca tgggtaaagt acttgtaaat aacttcccat gacagaaagt
    10681 ttatcaccag cttctataga ttttacagct actcctatct ttgataaacg cttaacgtac
    10741 tgcgaattcg ttaaagaacc tgaacttgta atcaaacgtg caactttaaa atgctttgag
    10801 ataacttcca tatcccccat atgatctgta tcggtatgag ttatcactaa attatctatc
    10861 ttgctaatac ctctgctttt caagtaagga atgagcgttc tctcagcatt agactggttt
    10921 actttttgtt tccattcttc aggctgttca aatctcaccc ttcccccagt atcaattaaa
    10981 attgtttttc ctaaccaatc cctcactaaa atactatctc cttgtccaac atctaaaatc
    11041 gcaacctcgt tagtaattgg attcttagtg ataaaaaaca aggtaaagat aataagaagg
    11101 gagcaataac gaaaacattt agaatgataa taatcataaa gtaatcccaa aattataatc
    11161 atgactatta aaagaaaaag gctgggcttt ccaaaaataa gtggtcttgg aaacagttgc
    11221 ccagtccatt ttaataatac ttctaggatt tcaaacaaac tattaataca acttaattta
    11281 acgaacggtg ataagacaaa tataacagtt aataaaggca aaaatataat atcaaatgca
    11341 aaagataaaa gtgctgttaa tataattgat attggttgaa aacccgaaaa atagtaggtt
    11401 agtatgggaa gaattcctac agagactgtc aaagattcga tagctacttt ttttatactc
    11461 gaaaaatgat caaaggaggt catcgtaagt ataaaagcat aagcaaagct aagaactcct
    11521 cccgtagtca aaagtgagtg ggggagagag ataagacaaa ttaacaagca gagagcaaag
    11581 ttctcatctt tcttgacacc gctatgtgat aataaagatt gaataagtgc cctaacgact
    11641 gagatactaa agcctgttaa accagcataa attagcgaga atggtatttg aagtaaccaa
    11701 acatggtcta gacgtaagcc aatacgtaga cagatataac gaaaaatacc gagaaaaaaa
    11761 cctacttgca tacctgacaa agcaaataaa tgtataatac ctagactact atataaatcg
    11821 gacatgtcat caaaggtctt atctagatat ccaaacaaaa gccctgacat atagtgacgc
    11881 ataggatttg gaaaagtttg actaataaca attgcacttc tacgccactt aaacataaga
    11941 tcaaagaaag acctatgttg aaataagcgt atctgtttta tctttgtcac tttggcgata
    12001 cgataaattc catgtctttt taaatactga cgataatcaa agccattaaa atgccttctc
    12061 tcctctgctt cttcaagttt gatatctgca attatcatta tattattatc aagattttga
    12121 aaataatctt tctcactttt attttttaat gtgtaaaaag cttgataagt gttacctgaa
    12181 gcatctgcct ggaatgataa aacatcacca ttgatagaaa tagtatcaat acgcacttta
    12241 atctggctaa tatgattcgg tactctttga tattggacat tcatattgtg attttgccat
    12301 aaaaaataaa ataagaaaaa aattactatt gttattgttt taaaaatagt ttcacgatca
    12361 tattgataaa tcagaaaaat aaaagtaaat aaaagaaagg cacaaccaag cattgtccaa
    12421 gaaaacacta gtaagtaaat ttggaagacc aacaatgcta aataaatagg ttttagagga
    12481 aaatacttag tcaagtgtaa catcttgacg taatttttct agtgttttct caccaatacc
    12541 tgatacattt ttgaggtcat caatagtttt aaaaccacct tgactatcac gataatctaa
    12601 aatatcttga gctcttttag ctccaacgcc tgatatggtc tgaagacttg ataaatctgc
    12661 tttattaaga ttaattttcc ccttactatt tccctgagta gcaatattat ttgctttctc
    12721 ctcttccaca acagttattt tttcaccttt ttgagcaaca tagataaccg tctcatcttg
    12781 taatttctgt gctagattaa cggatttact atcagcgaga ttgcttagtc cacctgcacg
    12841 cttaatcgca tctgttacac gactttgaga aggcaaacta taaacacctg gatgattgac
    12901 tgcccctttt acgtcaaccg ttacttgatt agatacttta tctttgacgt gactaatcat
    12961 tggtaccttt ttttctgtta tagttgatac ttttttctct aaattgatat ttaaatcgtc
    13021 ttctttatga tgagtgccaa aatgatttaa acccaaaatt ccaaacaata agagtcctac
    13081 aataattcct gttgtctccc atttatgcga tttaattttt tccaatacta tttcaaacat
    13141 aaaaatattc ctccatttac ttattcgtaa ctaaagagat tttcctatta aaaacaaaaa
    13201 aaactattta ctaaaaagta aatagtttcc atgtaaccct tattttagat gtttttgtgg
    13261 atcccacaca aagcttgcta aataactaaa aattagagtt aggacaagca agacaatagc
    13321 tataattgct aaaggtagac gataaatata agtcaatgga tttaatttct taccatcatc
    13381 ataagtttca ttttctctat ctaaacgatc aaattcttcc tgaatacgac gagatacttc
    13441 tgcaatccct tcggcatcca tacgcttaat atcagaaaca tcaatagggt taccaaaatt
    13501 catatcaaca cgatgcccct ttaatagatt tttaaaaacc ataggacctc tatatgctgc
    13561 tggcataatt ctgacttttg ccatttttgc aataacagct acgcctcctt tgacatcttt
    13621 ggagtggcgg cttccactcg gaaacatcac caaagaacgg ttactatttt tcagcatctt
    13681 tacaggatat cgaatagcgt cttgccctgg cttttcacga tcaataggga aagcaccgca
    13741 cattttaatc caccaaccaa acaaacgatt cgtgaataat tcttttttgg ccataaaaac
    13801 aaactgtttg ggtctagcag caaaagccat gtacacagga tcccaaaatg ttctgtgtgg
    13861 agctactaaa atataatttt catcatcttt taacatttta tcttcattat gatagtgcgc
    13921 attaccatta gccacccaaa tcaagaacat aactaaagta cgtaagtaag tatagaacat
    13981 gtttctcctc tacctttcat caagtaactt ctattttatc atgcttcctt ttttttagaa
    14041 agaattatct taaaatcaac ctccacactt tcaaaactag tgctaggaag ctgtaatttt
    14101 gctataataa gcctatgata gatacaattt taaaagaaaa tgaacgcatt gatcagcttt
    14161 tttcaactga tgtcaaaatc atccaaaaca aagaagtttt ttcctactcc atagatagtg
    14221 ttttattgtc tcggttccct aaacttcctt ctcgtggtct tatcgttgac ttatgcagtg
    14281 gtaatggtgc tgtcggtttg tttgccagca ccaaaacaag tgcgacaatt atagagatcg
    14341 aactacaaga gcgccttgcg gatatggcta agcgttcaat taagctcaat aaattggaaa
    14401 aacaggttac tatgattaat gatgatttaa aaaatcttct tgatcatgtt caacgttcaa
    14461 acgttgattt aatgctttgc aatccacctt atttcaaagc aagcgaaacc tctaaaaaaa
    14521 atctaagccc gcattatctc cttgcaagac atgaaataac aacaaacctt agagagattt
    14581 gtcaaattgc tcaacatgct ctaaaaacaa aaggacgtat cgctatggta catcgtccag
    14641 accgtttcct tgaaattatt gatacgatgc gtcagttcaa tttagcacct aaacgtatcc
    14701 aatttgttta tccaaaatta ggaaaggatg ctaatatgct tttaattgaa gctattaaag
    14761 atggctcgac agaaggtatg aaaatacttc ctcctcttgt tgttcatcaa gataatggag
    14821 actatactga gactattttt gatatatatt tcggggaaaa cggaaaatct catgattaaa
    14881 gttcctgctt atatgtatgt tttagaatgt tcagatggaa cactttatac aggttatact
    14941 acagatgtca agcgccgtct caatacacac aataccggaa aaggtgctaa atatactcgt
    15001 gcgagattgc cagtaaaact tctttattct gaagctttta atagcaaaca agaagcaatg
    15061 agggcagaag cattatttaa acaaaagact cggaaagcaa aattgactta tattaaacaa
    15121 cataaaaatg aacaataata cgataatata ctaagtgcaa tgagcctgtc ctcgcttctt
    15181 taacttttaa atttcaacct cactttaatc ttttgtcttc attcttaaag ttataaggca
    15241 attttacgga tatcactatc tacgctttat cttaaaaaat aaaaaaggag tcgctcagca
    15301 actccttttt cgtaatcctc ttatttatcg cctttgttgc gaataacaaa tccagtttgt
    15361 ttttcacttg aagtgttttt atgaggtttt ttattttctt gacgacgatt atctttcttg
    15421 aacttatcat cacgacgttt aaaatcacga cgacctccac gatcatcacg gtcacgattt
    15481 ccacgacgat caccacgatc attgcgtcga cgatcgccac cacggccatt acctcgtcca
    15541 cctttacctt taaagccacc acctgatggt ttaaatggta atggtttttc acgtgtgatt
    15601 tcaacttctg gtaacgattc tggatcctga actgttaagc ttaagacata aagagcaagt
    15661 tcttctggtg tatattgagt tgctaattct aaagcatcgg ctttaaattt atcaaattta
    15721 gaaactaatt cttgatcctc aaagtcacgt gcaatacgtt tcaatgcaac ttttttctta
    15781 gcctgaaatg cttcgcttgc agtagcaggc ttcattccag tcatacgttt tttagttaag
    15841 ttttcaataa ttgtcaaata tcccatttcg tttggtgaaa caaaagtgat agattgacca
    15901 gatttaccag cacgaccagt acgtccaata cggtgaacat aactttctgg atcttgggga
    15961 atatcatagt tgtaaacatg tgtaacgcct gaaatatcta aaccacgcgc agcaacatct
    16021 gtggcaacaa gaatatcaat atggtcattt ttaaagtctc tgataacacg aagacgttta
    16081 ttttgatcta agtctccatg aattccttct gctcggaaac cacgtaattt tagaccacga
    16141 gttaattcat ctacacgacg tttcgtacgt ccaaagacaa ttgacaattc tggttgatca
    16201 acgtccatca aacgtgtcat agtatcgaat ttttcatttt ccttaacgcg aacataatac
    16261 tgatcaacat tcacattagt taattcagtt gctttaattt taacatgctc aggatctttc
    16321 atgaatttaa ccccgatacg tttgattgga tcaggcattg tcgctgagaa taaaagagtt
    16381 tgacgtgctt ctggtacacg actaatgatt gcttcaatgt cctctaaaaa tcccatgtta
    16441 agcatttcgt ctgcttcatc aagaataaga gtttcgatgt gattaagttt aagtgcttta
    16501 cgtttaatta aatcaagtaa tcgacctgga gttccaacaa caacgtgtgc tcctgaacgt
    16561 aatgccttga tttgtttttc aatacttgac ccaccataaa cagagcggac cttaacacct
    16621 ttatcgcgtc caaaacggaa caattcttct tgtgattgta ctgcaagttc acgtgtagga
    16681 gcaataataa gtgcttgaat agtattatct tcagtatgaa ttttatttaa agttggtaac
    16741 ccaaaagctg cagtttttcc tgtacctgtc tgcgcttgac caatgacatc tttgccttca
    16801 agagcaagtg ggatagtcat ttcttggatt ggtgatggct ctacaaagcc tgctttttct
    16861 accgctgaaa gaatatcttg cgataagtta agttctgtaa atttcaaatg tttctctttt
    16921 ctaaaagaca gtgcgaagct gccttatgaa cctttattag ttatcttttc tgacaaccta
    16981 cttagtgtaa cacaattaaa agtaaaaaga tataaaataa ggtcaatcta ataaataaca
    17041 cgctttcata aattgctagt tctattagtt gcttcagaaa atagaaaaaa ggcaataaaa
    17101 accctccatc tataaagaag gagggttcat agtttatcta tctagaacgc aacgtctgct
    17161 tcacttggaa tctccttagt gatatagtga cgaatctctt tacttgttag tacttcttta
    17221 agtactttta ttttatcact atttttatta tcttcacgag ctaccaaggc acctgcaaaa
    17281 cggatatctg gttttttctc atataataat ctatcccttt taggaactag attgattttt
    17341 gtcacatatc cagggaaatt aaagacaagg tcatattcaa aataggcctg attgaggtta
    17401 agtaatgcta caggttcaat tcgtaatttt ttagggttag taataatatc ttcgatagcc
    17461 ttggtctttt tggacgtatt ctttaaggtg ataagtttct tttcttgcaa taatagcaga
    17521 gctctagtca tattggcagg atcagacgga atcgctacct tagcaccgtc tttaagctcg
    17581 gcaatatttt tcaaatgttg accataaaaa ccagccaatg aatgataaat tggtgtaatt
    17641 gacactagat gaccatcatt ttccttatta aagatactca tgaaaaattc atgttgtaaa
    17701 aggttagcat catgttcttt gttttctaaa gcaatgttag cctgaatata gtcagagacc
    17761 atgacaacct ttaaagtata tcctttttct tttacattat ctttaatcaa atctaaaaag
    17821 gtagatgtag gtttgctgta agttgcaaca gtaatcacct tttctttact attcttatct
    17881 ttaatttgct tagacgacat tgcttgctta gacgacattg cttgcttagt acatgccact
    17941 aaacataaag acatcatgat tagacatgtt agggaaagta attttttcat agttcctctt
    18001 ttcttttatt ttcttgagta gcggtttgct ataaaatagc ccaaactctg taagataaac
    18061 acatatatta taaataagac aacaatcaag tacataactg ggtagtcata ctcttgatag
    18121 ccaaaacgat aggcatattc acccaaacct cctgcaccaa taactcccat tattgtcgag
    18181 tagccaagaa tacttatagc cgttgcagta aatgataaaa caaggtcaat cttgatggag
    18241 gggattaaaa aataataaat tatctgtctc ttgttagccc ctagactgag agctcgatct
    18301 acaacaactt gaggtatatt taataaagct tgttccacat aacgtgcata caaactgact
    18361 gaaaccaaag taagaggtaa gattgccgct atcgttccaa atgaggtccc aaaaattaat
    18421 cgattgagag ggattaagat aaaaataaaa ataaggaaag ggacactccg taacgtacct
    18481 aaaaataaat ttagtagttg ataggctaaa gaatgtttaa tcaagtaaga ctttctcaat
    18541 gaaaacaata aaatacctgt tggaaaagca attagaaaac aaagaatcaa ggttaaccct
    18601 aacattaaat tggtttccca aaatgatttt aagatacctt cgccatgttt ttcaataatg
    18661 tcaatcatgt aacaaaaact cctttacttg atctacgtag tttgacttta attgattcag
    18721 agttgctttc actggaacaa cagtatctac aatagtctgg tgatccaaaa tcattacacg
    18781 atcacaatat tgttttaaaa gagataggtg atgagcaatc atcaagatag agataccata
    18841 tttttcacga agatcagaca acaagttaaa tatctctttt tcactattag tatctaaggc
    18901 agagctgatc tcatcacata gaataagttt cggtttttgc aaaacagcca tagcgattgc
    18961 aactttttgt tgctgcccac cactcaaact atggcatttc acctgtttta aatccatcaa
    19021 acctaaaaaa tctaagatat cattaattgc gtcatgattt actttttgat gatttaattt
    19081 ataaactaaa gctagatgat aatatacact ttctcctgct aacaaattat gattttgaaa
    19141 aatataagca atatctttac gcatctgacg taacttatta tcagaaaaag acttcacttc
    19201 ttttccttga taataaatat ttcctttatc ataagggatt aagccattaa tcatcttcaa
    19261 aatagtactc tttccagtac cattagaacc aatcagacca actatctcgc cttctgctat
    19321 attaaaagaa atatcatcta atctaaactg tgattttgga aaaattttcc caacactctt
    19381 taattctatt atcattattt aatactcctc aaaaaaatat cttatcaaaa aaatacagcg
    19441 ttttcaaaat atatgcttga ggcctactat ac
//
LOCUS       DATRSG010000039        19121 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.039, whole genome shotgun sequence.
ACCESSION   DATRSG010000039 DATRSG010000000
VERSION     DATRSG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19121
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.039"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            48..401
                     /gene="yaaA"
                     /locus_tag="VBD89_001311"
     CDS             48..401
                     /gene="yaaA"
                     /locus_tag="VBD89_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO7135836.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNK
                     DKSKTSKKPVRFPGT"
     gene            404..1513
                     /gene="recF"
                     /locus_tag="VBD89_001312"
     CDS             404..1513
                     /gene="recF"
                     /locus_tag="VBD89_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO7135837.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKSTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(1525..2391)
                     /locus_tag="VBD89_001313"
     CDS             complement(1525..2391)
                     /locus_tag="VBD89_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO7135838.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(2447..3109)
                     /locus_tag="VBD89_001314"
     CDS             complement(2447..3109)
                     /locus_tag="VBD89_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135839.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     EQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVAKAIVFADLANKV
                     SQ"
     gene            complement(3185..4666)
                     /gene="guaB"
                     /locus_tag="VBD89_001315"
     CDS             complement(3185..4666)
                     /gene="guaB"
                     /locus_tag="VBD89_001315"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO7135840.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMNTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(4823..5308)
                     /locus_tag="VBD89_001316"
     CDS             complement(4823..5308)
                     /locus_tag="VBD89_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7135841.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(5318..5998)
                     /locus_tag="VBD89_001317"
     CDS             complement(5318..5998)
                     /locus_tag="VBD89_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7135842.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
     gene            6206..6910
                     /locus_tag="VBD89_001318"
     CDS             6206..6910
                     /locus_tag="VBD89_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO7135843.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNKVIQTYRKAYQTFKTKKGARSSIEALLKRINSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNIPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNRREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
     gene            7176..8408
                     /gene="arcA"
                     /locus_tag="VBD89_001319"
     CDS             7176..8408
                     /gene="arcA"
                     /locus_tag="VBD89_001319"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO7135844.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            8414..8844
                     /locus_tag="VBD89_001320"
                     /pseudo
     CDS             8414..8844
                     /locus_tag="VBD89_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8860..9873
                     /gene="argF"
                     /locus_tag="VBD89_001321"
     CDS             8860..9873
                     /gene="argF"
                     /locus_tag="VBD89_001321"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7135845.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            9936..11363
                     /gene="arcD"
                     /locus_tag="VBD89_001322"
     CDS             9936..11363
                     /gene="arcD"
                     /locus_tag="VBD89_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO7135846.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTVVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVRYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            11384..12340
                     /gene="arcC"
                     /locus_tag="VBD89_001323"
     CDS             11384..12340
                     /gene="arcC"
                     /locus_tag="VBD89_001323"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7135847.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQADSDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVVN"
     gene            complement(12460..13485)
                     /gene="trpS"
                     /locus_tag="VBD89_001324"
     CDS             complement(12460..13485)
                     /gene="trpS"
                     /locus_tag="VBD89_001324"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO7135848.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(13593..14285)
                     /locus_tag="VBD89_001325"
     CDS             complement(13593..14285)
                     /locus_tag="VBD89_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7135849.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
     gene            14529..15401
                     /locus_tag="VBD89_001326"
     CDS             14529..15401
                     /locus_tag="VBD89_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7135850.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            15467..17086
                     /locus_tag="VBD89_001327"
     CDS             15467..17086
                     /locus_tag="VBD89_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7135851.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIIL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            17209..>19121
                     /locus_tag="VBD89_001328"
     CDS             17209..>19121
                     /locus_tag="VBD89_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO7135852.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DSFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKVGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILHFLSLTALFIQNYYFGFMTTIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEIPRRTRLAYGFSLIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILI
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWVFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIRKAQDKQTILYENQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFEHLIP"
BASE COUNT         6327 a         3398 c         3166 g         6230 t
ORIGIN      
        1 ttttatgcta taatgatgtg atatctaaaa ttggagatta aaaagtcatg gattataaat
       61 tatttgatga atatattact ttacaatcac ttctaaaaga gattggtatc attcaaagtg
      121 gcggagccat taaaaaattt ttagcggata atcaagtatt gtttaacggt gaccttgaaa
      181 ataggcgtgg aaagaaactc cgcctggggg atattattac tattcctgat caaaatatcg
      241 agatcattat aaggaagcca tcagatcaag aaattgaaga acgtaatgta gaaatagctg
      301 aaaaacagcg tgtttcagca atcgttaaag aaatgaataa aaacaccaac aaagataaat
      361 caaaaactag caaaaaacca gttcgctttc cagggacata aaaatgtgga taaaaaatat
      421 ttctttaaaa cattatcgta attatgaaga agcacaagta gatttttcac ccaacctcaa
      481 tattttcatt ggccgcaatg ctcaaggaaa gacaaatttt ttagaagcaa tctacttctt
      541 ggctttgact cgaagtcacc gtacccgttc tgataaagaa cttgtacatt ttaaacacca
      601 tgatgtacaa attacaggtg aggtaatccg caaaagtggc catcttaact tagatattca
      661 attatctgaa aaaggaagaa tcactaaagt taaccatctt aaacaggcaa aattatctga
      721 ttatattggg gctatgactg tcgtactttt tgcccctgaa gatcttcaat tagttaaagg
      781 tgcacctagt ctaagacgaa aatttttaga tatagatatt ggacaaatta agtcaacgta
      841 cttagctgaa ctttcaaatt acaatcatgt attaaaacaa agaaacactt atctaaaaac
      901 tactaataac gtcgacaaaa cttttttaac agtccttgat gaacaattag ctgattatgg
      961 aagtcgtgtc attgaacacc gttttgattt tatccaagcc ctaaatgatg aagcggacaa
     1021 acatcactac attatcagta ctgagttaga acatttatct attcactaca aatcgtcaat
     1081 agagtttaca gataagtcaa gtattaggga acatttttta aaccaattat ctaaaagcca
     1141 ctcaagagac atctttaaga aaaacacaag catcggacct catcgtgatg atattacttt
     1201 ctttattaat gatatcaatg ctacttttgg tagccaaggg caacaaagaa gccttatttt
     1261 atcattgaaa ttagcagaaa ttgagctaat aaaaaccgtt accaacgatt atccgattct
     1321 cttacttgac gacgtcatga gtgaacttga caaccatcgt caactaaaac tcttagaggg
     1381 aatcaaagag aatgttcaaa catttattac tacaacaagt ttagaacatt taagtgcttt
     1441 acctgatcaa ctcaaaatct ttaacgttag tgatggtact attagtatta atgaaaaaaa
     1501 agcaacagat taatctgttg ctttttaata agatttaaca ataccaagta atatagcacc
     1561 cataacaaaa caaaggatac ccatgacaac ccatttctgc tctttcttcg tttttgtctc
     1621 acctaaaaat aaaataccac caataataga gataattact ccaagttgag aaaaactaaa
     1681 agcaattgct agccctgctt tagctgctgc caataacatg aagacattac caacgcccca
     1741 catgagacct gtaatcatat ttttaacaac aacagcctca aagttaacac gaaacttcat
     1801 gaaacaaatt gcacctagac acattccaac agccatgggt aaaatgacgg ccatagcgtc
     1861 gaacttcata atgttgttaa ataaaactgc gtacgagatg taacctacag ttgaataagc
     1921 aattgtagca aatccttttg aaaactcttg atgtgttgcc aaagcttgtt ctgaaacatc
     1981 acgtttactt gagaaataga agccgataac taataatgtc aacgctgtca atcctaaaat
     2041 aaattggatt ggcttagtcc attcatgaaa gactaaagca ccaactaggc ttccacctac
     2101 taattgtgca ccacttgaca gtggattagc aacagagact cccatatatt tcattgcttg
     2161 aaattgacca ttttggccga ctgaccatag gataccacct aagataccaa aaatccacaa
     2221 tgaggcagtc atctctggct gtttaaataa ccatacgata atcgcaaata gcaatgctcc
     2281 taaagtcatt ccaaatgttt gttgatttgg acgccctcca attttattac taacaaatcc
     2341 aatacttccc cacgcaaaca tgggaatcaa tgcaataagt aaaccttcca attctttctc
     2401 cttacaatgg aatattaagt attacctatt ttcaatccta gagaatctat tgactaactt
     2461 tattagcaag atcagcaaat actatagctt tagcaacact atctttttcc aaagctataa
     2521 tgacttgttt tatcaataaa ttgaccagtg atttagaata aggtaactgt tctttagtca
     2581 tattactaat cttatttaaa ataacatctt caggtaaaat atcttgaaaa tagcagatga
     2641 ctttgaaata aaaatcattg tactgtgtca caccattcag ataagtaact accaggttaa
     2701 tctcatcttg aggcagcctc atatttaacc ctaaatttga aataaaaagt ttagccaata
     2761 ttttgagaac attgctttct attctatcac aggattgttg ttcttggaaa ctgaaaattc
     2821 tcatgatatc ttcattatct cgataagatt ccagtaaagt taattgctct aaatcagtat
     2881 tagaagttcc agccttaact tttgaatcta gaatggatac ttcattgata gttaccccaa
     2941 cctccaacgt ttgtaaagct ttaataaatt tatcaatacc tatttgtgtc tcaccatttt
     3001 caaaacgtga tagagttgat tttgataatt gagcactttt ggctagagaa cttagggata
     3061 tacctttgtc ttctctcaac tcttttaaaa tttttccata atttttcata caaaaacttt
     3121 actatatttg tttcttttat gcaacaaaaa gcatcctcat aggggatgct tttttgtcct
     3181 tattttagtg tactgaataa tttggtgcct catttgtaat ttgaacatca tggggatggc
     3241 tttctttcaa accagctcct gacatttcaa caaattgagc attatcatga agttctttta
     3301 tgttcgctgc acctacatat cccataccag aacgaatgcc accaagcatt tggaaaacaa
     3361 tatcggctac tgaacctttg tatgctacac gaccttcaat accttctgga accaatttgt
     3421 tagcctcgtt tacagaacct tggaaataac gatcgcttga accttttttc attgctgcaa
     3481 ttgagcccat accacggtat gtcttgaact tacgcccttg gaaaatttct gtttctcctg
     3541 gcgcttcatc tgttcctgca aacattgaac ctagcataac tgcatttcca cctgctgcaa
     3601 gtgcttttac aatatcacct gaatatttaa taccaccatc agcaatgata gtcttaccgt
     3661 actcacgcgc aacagccgct gcatcgtaaa tagctgtaat ctgaggaaca cctaccccag
     3721 caacgacacg agtcgtacaa attgaacctg gaccaatacc aactttaacg acatccacac
     3781 ctgcatcata aagtgcacgc gctccttctg ctgtagcaat atttcccgca atcaatgtgc
     3841 gattagggaa gtgagcacga atttcagcaa ttttacggag cacaccagct gaatgtccat
     3901 gtgctgtatc aatgacaata gcatctgcac ctgcttcaaa caaagcttct gcacgttcaa
     3961 aagtatcaga ggttacacct actgcacctg caactaagag acgtccaaat tcatcttttg
     4021 ctgccttagg aaattcaata actttttcga tatccttaat agtaataagt cctgaaagac
     4081 gtccctcatc atctactaaa ggtagctttt caatacgatg ctcatgaaga atacgctccg
     4141 cagtttcaag atctgtacca ataggagctg ttacaagatt ttgacttgtc atgtgctctg
     4201 agattagttg tttataatca gaaatgaaac gcatatcacg gttagtaata attccaacta
     4261 atttacgatt ctcgagtgtc tctacaatag gaactccact aatgcgatag ttttgcatca
     4321 attcctcagc ttcagagaca gtattatctg gtgtcaagaa gaatgggtca ataataacac
     4381 cattttctga gcgtttaact ttacgaactt cttctgcttg atcaactatt gacatatttt
     4441 tatgaatgat accaagccca ccagcacgtg caatagcaat agccatctta ctatctgtaa
     4501 ctgtatccat tgcagctgta atgattggaa tgtttaatgt caaattatca gccaactttg
     4561 tattcatatc aacttcattt ggtaaaacat ggctttcagc aggaataagc agaacatcat
     4621 caaaagtaaa accttttttc aaaaacttag tatcccaatt tgacatcaaa aaatcctctt
     4681 ttcttagata gtagatataa aaaatcccat taaaattata ttgttactat catatcacgc
     4741 taaaatcatt tgtcaatata aaaagagcat agaattaata gcttctagct ctttaaagta
     4801 atgaaaaaat cttcttaatt ttctattttt ttgagaagaa gaaaggcggc gtatactgac
     4861 ttaatttctc aaaacacttt tgagcttctt cactattatt acaaataatc aaacaagtat
     4921 catcaccaca gacagttgct aagatctcag gtaataaaat cgaatctaat atggagccaa
     4981 aagattgagc taagccaggt agtgtcttca aaacgatttg gttttgaact ggaaacagca
     5041 tgactagggc atcttccatg taaaaacgta accgttcttc ccaacgcttc tgagatatac
     5101 tatgaatctc ataataagtt tctgtagcat tatcactgct ttcgttaacc ttaattaaat
     5161 taagttcctt catatctcga gataatgttg cttgcgtcac tgaaacgcca tttttttcta
     5221 gtaattccct taactcatgt tgtgtacgca ccttagtctc agagactaag gaacgaatta
     5281 attggtgtct tgtttctttt ttattcatat aaactcctta ttcaatgaat ttcttgaaat
     5341 aatttttatt attatagaga agcttctttc ctttcatttc aacgatatct ttttggcgta
     5401 attcctttaa aacatgacta actgtctcgc gagtagttcc gctcatattg gcaatttcaa
     5461 tcgttgtaat aggaaaaggt aaatgtcccc tttctgtagg aacttgcaaa agtagagttg
     5521 caagcgattg gataactctc atactggcac ttgaagtcac catattacga agtcgtattt
     5581 catgaactcg taatagctta gaatatttcc gacaaagatg cttcatttga ttgatatttt
     5641 gaagagagta ttcttcgtat aaagccattg gcagagaaaa atactcaata tccgtaatag
     5701 ctactgctga aaaatggtaa tcatcatcca aaaagagacc tccataagga aaaactgtat
     5761 cttgtctaac ataatctgta taaataaaag aaccagaagc gtctgattgt tcaattttta
     5821 catgtcctga ctggattaaa aacagttttt cacggtaatc accttcaaaa aataaggtat
     5881 gattttttag agctgtacga tgtttaatgt ggctcacaat atggtcaaat tgttcaatag
     5941 taaagtgttt aaaatcatct agttgtctga aataaaaata ttgttctttt gtaatcacct
     6001 gattttctag acctttctaa tcattagatg cgaacatttt ttgtataatg aaaatttttt
     6061 aatgtataaa attttcttgt cacgataaat cctccttcat tatagcaaaa aaattcgtag
     6121 aaagaacgtt tttcgttgaa ttttgaaaac gcttttgaga aaatgtaatt gttctgatat
     6181 cttttaaaaa aattaagagg ccttcatgac taaatttatt gttgattcat cgtactggaa
     6241 tttgtttcca acttctaaaa ttggagtaat cttaatcaaa gattatcaca tggacaggaa
     6301 ccttgaaaca gaacttaaac agttactgtc tgatagtcat tctttagcaa aaaaatattt
     6361 gcaagagaaa gaattcagtc aaaacaaagt tatccaaact tatcgcaaag catatcaaac
     6421 ctttaaaact aaaaaaggtg cacgttcaag tatcgaagca ttattgaagc gaattaattc
     6481 aggaaacgaa attactagta taaatcctct agttgatatc tataatgccg ctagccttag
     6541 gtttggatta cctattggtg ctgaggatag tgatacattt agaggtgatt taaaactaac
     6601 tattaccaat ggtggtgatg aattttactt gattggcgaa gattttaaca taccaactct
     6661 ttcaggtgag cttgcctatg ttgatgatgt tggagcagtt tgccgctgtt ttaattggcg
     6721 tgatggaaaa cgtactatga ttaccgataa tactcagaac gcttttttag ttatcgaatt
     6781 aattgataat agaagagaaa ttatttttaa agaagcgttg gactttatcg ccaccaatac
     6841 aaatcgcttt ctaaaagcta aaacacagac catcatatta gataaagaac acagtgaaat
     6901 aacgctataa cattaaaacg aaaagtaaaa attttacttt tcgttatttt ttaggttaat
     6961 aatttctcac tttccaacga taaaagaaag aaattgtgta gaaatcgctt tcatttttat
     7021 aaatttaaat ttttttggat ataatcgtga cattcttaac acttttaatg cgaataactt
     7081 ccctatttta tattcaaaag aaagtagaat gtaagtgtag atatagagat aaatcctgtt
     7141 tcaatgcaaa tctctaacta agaaaggagt aaaatatgac tcaaacacat ccaattcatg
     7201 ttttctcaga aattggaaaa cttaaaaaag ttatgctaca ccgtccaggt aaagaaatag
     7261 aaaatctgat gcctgattat ttagagagac tcttgtttga tgatatccca tttttggaag
     7321 atgcacaaaa agaacacgat gcgtttgcac aagcacttcg caacgaagga gtcgaagtac
     7381 tttaccttga aaatcttgct gccgagtcgc taacaaacca agaaattcgt gaacagttta
     7441 tcgatgagta cattggcgaa gcaaatgttc gcggtcgtgc tactaaaaaa gctattcgag
     7501 aattattact caatatcaaa gataataaag aacttatcga aaaaactatg gcaggtatcc
     7561 aaaagtctga gctacctgaa atcccatctt ctgaaaaagg tctaactgat cttgtcgaat
     7621 ctaattatcc atttgccata gatccgatgc caaatcttta ctttacacgt gatccatttg
     7681 caactattgg gaatggagtt tcattaaacc atatgttctc tgaaacacgt aatcgcgaaa
     7741 ctttatacgg taaatatatc tttacacatc acccagagta cggtggaaaa gtccctatgg
     7801 tctatgatcg tgaagaaaca actcgtattg aaggtggaga tgagctcgtc ctttctaaag
     7861 atgtccttgc cgtgggtatc tctcaacgta ctgatgctgc ctctattgaa aaactcttag
     7921 tcaatatttt taaacaaaat ttaggtttta aaaaggtttt agcatttgaa tttgcaaata
     7981 atcgtaaatt tatgcacctt gacacagtct ttacaatggt tgactatgac aagtttacaa
     8041 ttcacccaga aattgaaggc gatcttcgcg tttactctgt aacttacgaa aaccaagatc
     8101 ttcatattga agaagaaaaa ggtgatttgg cagatcttct agcgaaaaac ctaggagttg
     8161 agaaagtgga attgattcgt tgcgggggtg acaacttagt cgctgctgga cgtgaacaat
     8221 ggaatgatgg ttcaaataca ttaactatag caccaggtgt cgttgtcgtt tacaacagaa
     8281 atacaatcac aaatgcaatc ttagagtcca aaggattgaa attaattaag attaatggaa
     8341 gtgaattagt tcgtgggcgt ggtggaccac gctgcatgtc aatgccattt gaacgtgaag
     8401 acctttaaaa tttatgaagc tattagtcaa tcacttgcct taccaaagag ctgcaagcct
     8461 atatattcgc ttttctgttt tcgttatcga aagaaatata aaaatgaaag aagaattcga
     8521 tgataatgac gagcaagata ctatttatgc tgttctttat gatggaaaca gccagtttca
     8581 acaggacgct ttcttcctga aactcaaaca gaagctcgtt tgactcgaat tgcgacttta
     8641 aaaggttatc gtggtaacgg ctatggtacc aaaattatta tagcactaga aaattatgca
     8701 aaagaaaatg gttatcacta tctaactata catgctgaat tgacagcaaa agatttctac
     8761 cagacacttg ggtatcaagc tactgggaat atttatatgg aagacgggga agcctgtcaa
     8821 acactcgaaa aatatttaat ttaaaggaga cattaacata tgacacaagt atttcaagga
     8881 cgtagtttct tagcagaaaa agatttttct cgtgaggaat ttgaatatct tattgatttt
     8941 tcagctcatt taaaagacct taaaaaacgt ggtgttcctc atcattatct tgaaggtaaa
     9001 aatattgctc tcttatttga aaaaacatct actcgtactc gcgcagcctt tacaactgca
     9061 gcaattgacc taggcgctca tccggaatac cttggtgcaa atgatattca acttggtaaa
     9121 aaagaatcaa cagaagatac tgctaaggtt ttaggacgta tgtttgatgg tattgaattc
     9181 cgtggtttta gccaaagaat ggttgaagag cttgctgaat tttctggagt acctgtctgg
     9241 aatggtttaa cagatgaatg gcatccaaca caaatgctag ctgactacct tactatcaaa
     9301 gaaaacttcg gtaaacttga aggtattact cttgtttact gtggtgacgg acgtaacaat
     9361 gttgccaact cgcttttagt ggctgggact ttgatggggg tcaatgtaca catcttttct
     9421 ccaaaagaac ttttccctgc tgaagagatt gttaaattgg ctgaaggata tgccaaagaa
     9481 tctggggctc acgttctcgt tactgataat gtagacgaag ctgtaaaggg agcagacgtc
     9541 ttttacactg atgtctgggt atcgatggga gaagaagata agttcaaaga acgcgttgaa
     9601 cttcttcaac catatcaagt aaacatggaa ctgattaaaa aagctaataa tgataatctt
     9661 atcttcttac actgcttacc tgcattccat gatacaaata ccgtttatgg caaagacgtc
     9721 gctgaaaaat ttggggtcaa ggaaatggaa gttactgatg aagtcttccg tagcaaatat
     9781 gctcgtcatt tcgaccaagc tgaaaatcgt atgcacacta ttaaagctgt aatggctgca
     9841 acccttggaa atcttttcat tccaaaagtt taaattaaac gactataggg agttgacata
     9901 tagtcaattc cctattttag gaaatgaggt ataaaatgga aaaagaaaaa aaattaggtc
     9961 ttttaccact aacaatgctt gtcattggct ctcttatcgg tggcggaatc tttgatttaa
    10021 tgcaaaatat gagttccaga gccggtttgg taccaatgct tattgcttgg gtaattactg
    10081 ctatcgggat gggaactttc gttttaagtt ttcaaaattt atctgaaaaa aggccggacc
    10141 taacagctgg aatctttagt tacgctaaag aggggtttgg aaactttatg ggatttaact
    10201 ctgcatgggg ttattggtta tcagcttggc ttggaaatgt tgcctacgct gcactcttat
    10261 tcagttcact cggttatttc tttaaattct ttggtaatgg aaataatatc atctcaatta
    10321 ttggagcaag tatagttatt tgggttgtcc atttcttaat tttaagaggt gttaatacag
    10381 ctgcatttat taataccgta gttacctttg caaaattagt acctgttatt attttcttaa
    10441 tttcagcgtt attagctttc aaatttaaca tttttagtct tgatatctgg ggaaatggat
    10501 tacatcaatc aattttcaac caagtcaatt caactatgaa aaccgctgtt tgggtattta
    10561 ttggtattga gggcgccgtt gtcttctcag gtcgtgctaa aaaacactct gatattggta
    10621 aagcaagtat cctagcatta ttcactatga tttcacttta tgtattgatt tctgttttat
    10681 cacttggtat catgtcacgt ccagaacttg caaacttaaa aacaccagct atggcttacg
    10741 ttctagaaaa agctgttggt cactggggtg ctatcttagt taaccttggt gttatcattt
    10801 cagtatttgg cgctattctt gcttggactt tatttgcagc agaattacca tatcaagctg
    10861 ctaaagaagg tgcttttcct aaattttttg caaaagaaaa taaaaacaaa gctccaatca
    10921 actcactctt agtcactaat ctttgtgtac aagcattctt aatcacgttc ttattcacac
    10981 aaagtgctta tcgttttggt ttcgcattag catcatctgc tatcttaatt ccttatgctt
    11041 ttacagcact atatcaatta caattcacac tccgtgagga taagtcaact ccaggacatc
    11101 aaaagaattt aattatcggt atcctcgcta caatctatgc tgtttacctt atctacgctg
    11161 gtggttttga ttacttactt ttgacaatga ttgcttatac tctaggtatg attctctata
    11221 ttaaaatgag aaaagatgac aagcttccta tctttgtgag atatgaaaaa attagtgcca
    11281 ttgtcattct agcgctttgc ttactatgta ttattgaaat tatgactgga caaattgaca
    11341 ttgctgcaat cctagcaggt tagtcattag aaagagggat aaattggtaa aagaacatca
    11401 aaaaatcgta gttgccttag gtggtaacgc tattctatca acagatgctt cagcaaaagc
    11461 acaacaagaa gcattaatca acacatcaaa atcactggtc aaactgatta aagaaggcca
    11521 tgatgtcatt gttactcatg gaaatggtcc ccaagttggt aacttattgc tacagcaagc
    11581 agattcagat tctgaaaaga atccagcaat gcctttggat acttgtgttg ccatgacaga
    11641 aggaagtatt ggtttttggt tacaaaatgc tcttaacaat gaattacaag aacaaggcat
    11701 tgacaaagag gttgcaacag tggtaactca agttattgta gatgaaaaag atcaagcttt
    11761 cactaatcca acaaaaccta ttggcccatt cttatctgaa gaagatgcca agaaacaagc
    11821 ccaagaaaca ggttcaaaat ttaaagaaga tgctggtcgt ggatggcgta aagtcgttcc
    11881 ttcaccaaaa ccggttggta tcaaagaagc ttctgttatt agacggttag tggactctgg
    11941 tgtggttgtt attagcgcag gtggcggagg tgtaccagtt attgaagatg ctaatacaaa
    12001 agcattaaaa ggggttgaag ctgttattga caaagacttt gccagtcaaa ctttatcaga
    12061 acttgttgat gccgacttat tcatcgtctt aactggcgtt gacaacgttt ttgttaattt
    12121 taataaacct aatcaagaaa aattagagga agttactgtt tcacaaatga aacaatatat
    12181 cactgaaaat caatttgccc ctggcagtat gctaccaaag gtagaagctg cgattgcttt
    12241 tgttgaaaat aaaccagaag cgcgtgctat cattacttca cttgaaaata ttgataatgt
    12301 cttagcagaa aatgcaggga cacaaattgt tgtaaattaa acaatgtaga ttaccattac
    12361 atacactcct tattaataac aaagtaatgt cactccttag gcattcctat aataaaagag
    12421 agctagatat atctagctct cttttattat aggaatatat tatttaaaat agttcaaacc
    12481 catggctgat ttaacctcat ccaaagtaga ggctgcaaca gcttgagctt tctcactacc
    12541 tttttgtaac atttgataaa cttgtcccat atctttagca tattcaaggc gacgttctct
    12601 aataggtgat aattcacgct ctaagatatc aagtagataa cgttttgttt taacatctcc
    12661 aagtccacct ttttgatagt gttctttcat agcagtaatc tctttttggt cttcatctct
    12721 tccaaaaaca tcaaggtaat ggaataccat attaccttcg atttgtcctg gttcttctac
    12781 tttaatatgg tttggatcag tatacatgct cataactttt ttcttaactg tatccatatc
    12841 atctgctaaa aagattccat tatttaatga cttagacatt tttgcattac catctaaccc
    12901 tggcagacga cctgctgcat cattttctgg atagattccc tctggttcaa ctaaaacctg
    12961 acaattataa gcatgattga aagatctaac tatttcacgt gtttgttcaa tcattggttt
    13021 ttggtcagtc cctacaggaa caaggttagc tttaaaagcg gttatatcag ctgcttgtgc
    13081 aacaggataa actaagaatc ctgctggaat agactcacca aaaccttttt gagcaatttc
    13141 tgtttttaca gttggattac gttccaaacg tgcaagtgaa accaagttca tataatacat
    13201 acttaattct gctaactcag gaatttgact ttgaatgaat aaagtcgact tgtttggatc
    13261 cagcccaaca gctaaataat caagtgcaac attaccaatt gattcaacaa tagtttgcgg
    13321 atctttagca tggtctgtca gtgcttgttg gtctgctaaa aagacaaata aagtatagct
    13381 accctcattt tgaagtagaa ctctattttt taagctacct acataatgtc caatatgcaa
    13441 ctttcctgtc ggtctatcac ctgttaagat aattggtttt gtcatgttta ctccttaata
    13501 taccaaagat atcatttcat atagttatct tatcatgtca caagccctat tgcaattcat
    13561 ccctacaaga aacattcacc ttattaagat gattaaacat tatgtaacgt taaatcgttc
    13621 ttaataattt caatcgtata atgaatcata ataattaaaa gcgttatcgt cacacctaaa
    13681 atagctctat atgcaaagat atcgtaaact attgcagcta ccaatgctcc aataataaat
    13741 gctagaacta ccagtgaata attgatagct cgtctttttt gcatagccgt tctctgacga
    13801 ttaacaatat actcatacca aaattgtagc attttccgat aattaccaga agtcattaag
    13861 atagtgtatg gttcagattc aatgcgactt cctgaaaatg tcaacattaa aatacctgtg
    13921 ctaaaggcta ataaagctac ccagatattg tggtttacat tttcaacaaa aggaataaac
    13981 atcgtagaaa gtaaaagggg acataatgca tatagacgcc aaaaagctgt tactgcacgt
    14041 tctttaatca caataccaac caaaaaacca attgaaaaaa agaaaataga aaataattta
    14101 gtgataaaaa agtgaaaatc attttgtccg agatttgcca ccatcaagat aatattacct
    14161 gtctgtgttg caacaaggga gtgaaactgc atgtgactat aaacgtcaat aaaaccacca
    14221 ataaaaccca ataatacagc catttttctt ccatcttgtg gcattataat atcatctctg
    14281 cgcattatag caaattctcc ttcttgtatt atcatgtttt ctattatata ccaataaaat
    14341 aggcattcaa ctcaaaaata tcgagaaatt tcttttctaa atttacttga ctaccaagtc
    14401 tcgctgttag ataataggta ataataagtt ttgataaaat aaaaatgtta tgactgagtt
    14461 gtcaatatta tttattgaca gctttctctt tttatcatgt tattattact aaagttaagg
    14521 agcttatcat gtctaaaaaa attaagaatt ttatactatt aaatattgga tctctaattg
    14581 ttgcatttgg tttcaacgct ttctttcttc ataatcatat tgtcacgggg gggattagtg
    14641 gtttggcagt tagttttcat gaagtatttg gttgggattc tgcttttttt attatgatta
    14701 tcaatattcc attgctcctt ctttgctact ttggcttagg taaacaaacc tttttaaaaa
    14761 ctgtctatgg ttcttggatt tttcctgttt ttattaagtt aacacaaagt gtaccaactt
    14821 tgacccacaa cccactcctc gcagcacttt ttggaggtgt tattgtagga tgtggtttgg
    14881 ggattgtttt ttggagcgac tcttcaactg gtggaacggg gattatcatt caattcttag
    14941 gaaaatatac tcctataagc cttggacaag gggttatatt gattgatgga cttgttacaa
    15001 ttgttggttt cctagctttt gacagtgata cggttatgtt ttctattatt gggttgataa
    15061 ctattagtta tattattaat gctatccaaa ctggatttac aaccttaagc actgtcttaa
    15121 tcgtttctca agagcaccaa aaaattaaga catatatcaa tactgtcgca gatagaggag
    15181 taacagaaat tcccgttaaa gggggatatt ctggaactaa tcaaatcatg cttatgacaa
    15241 ctattgctgg ttatgagttt gctaaattac aagaggcaat agcagaaatt gacgaaacag
    15301 ccttcataac agtaactcca acatcacaag cttctggacg tggatttagt cttcaaaaaa
    15361 atcatggacg tcttgatgaa gacattctta tgccaatgta atcgattgat aattaaagct
    15421 ttttctagta gaatagcaga tagaatatac acaagaggta acaaaattgc ttacagtttc
    15481 agatgtttca ctacgtttta gtgatcgcaa attatttgat gaagttaaca ttaactttac
    15541 agcaggaaat acctatggtc ttatcggagc aaatggcgct ggtaaatcaa ctttccttaa
    15601 aattttagct ggtgatattg aaccaacaac gggacatatt gcacttggcc cagatgaacg
    15661 cctttctgta cttcgccaaa atcattttga ttatgaagat gagcgcgtca ttgatgtcgt
    15721 tatcatgggt aatgaaacac tttatagtat catgaaagaa aaggatgcta tctacatgaa
    15781 agaagatttt tctgatgaag atggtgttcg cgctgcggaa ttagaaggag aatttgctga
    15841 actcggtgga tgggaggctg aaagtgaggc ttctcaatta ctccaaaatc taaatatttc
    15901 agaagagctt cattatcaaa atatgagtga attagctaat ggagacaaag ttaaagttct
    15961 ccttgcaaaa gctctctttg gtaaaccaga tgtacttctc cttgacgagc ctaccaatgg
    16021 acttgatatc cagtctatta cttggttaga agatttcttg attgactttg aaaatacagt
    16081 tattgtagtt tcccacgacc gtcatttctt gaataaagta tgtacacata tggctgacct
    16141 tgattttgga aaaattaaac tgttcgtcgg caactatgat ttctggaaag aatcatcaga
    16201 attagcagct cgtttacaag ctgatcgtaa tgctaaagca gaagaaaaaa ttaaacaact
    16261 tcaagaattc gttgcacgat tttcagcgaa tgcttcaaaa tcaaaacaag caacttctcg
    16321 taaaaaaatg cttgataaaa ttgaacttga agagattgta ccatcaagcc gtaaataccc
    16381 attcgttaac tttaaagccg aacgcgaaat gggaaatgat ttattgaccg ttgaaaatct
    16441 ctctgtcact attgatgggg aaaaaatcct tgataatatc agtttcattc ttcgtcctgg
    16501 tgataaaact gcacttattg gacaaaatga tatccaaact actgcactta ttcgagcatt
    16561 gatgggggat atcgaatatg aaggaacaat caaatgggga gtaacaacta gtcgttctta
    16621 ccttccaaaa gacaattcac gagactttgc ttctggcgag tcaatcttag aatggcttcg
    16681 tcaatttgct agtaaagaag aagacgataa taccttctta cgtggcttcc taggtcgtat
    16741 gcttttctct ggagatgagg ttaacaaatc tgttaatgtc ttatctgggg gtgaaaaagt
    16801 acgcgttatg ctttcgaaat taatgctatt aaaatctaat gttcttgttc ttgatgatcc
    16861 aacaaaccac ttagatttgg aatctatttc aagtctcaat gatggactaa aagatttcaa
    16921 agaatctatt atttttgcaa gtcatgacca cgagtttatt caaactttag cgaaccatat
    16981 tattattctt tctaaaaacg gagttatcga tcgtatcgat gaaacttacg atgaatttct
    17041 tgaaaatacc gaagtacaag ctaaggtcgc tcaactctgg aaataatcct ttggcatatc
    17101 tctaacctaa taaacgtgtc aagattgaaa atcttaaaac tatattaatc atttaaataa
    17161 aaaggttgga acaaatagcg tccagccttt ttatcttagg agatatatat gacaattaac
    17221 cactataagc ttcgtatacc ttactataca ataagctttc tacttccctt cataatcata
    17281 gtctgtatcc tctttactaa gaatatttat tggggaagtc caacaaccat attagcaagc
    17341 gatagctttc atcaatatgt tatctttaat caagcattaa gaaatattct tcatggttca
    17401 aacagcttat tctatacctt tacaagcggt ttaggtttaa atttttatgc tctgagtagt
    17461 tactatttgg gaagtttttt atccccaata gtttatttct ttaacctaaa aaatatgcct
    17521 gatgctatct accttttaac aatatgtaaa gtagggttga taggtttgtc aatgtttgtc
    17581 actctttgta aacgtcattg taaagttaat cgtgtcttac ttttagtaat ttctacttgc
    17641 tatagcttaa tgagtttctc aattagccaa attgaaatta atatgtggct agatgtattt
    17701 attctaattc cccttgttgt tttaggagtt gatcagctac tatgggaaag aaaacctata
    17761 cttcattttc tctctctgac agccttattc atccaaaact attattttgg ttttatgact
    17821 accatcttta ctagccttta cttcatcgta caaataaccc gtaatactga tagtaaggtt
    17881 gcatttaaac aatttcttca ttttaccttt ttatctcttt tagctggtat gactagtagc
    17941 attatgattt tgccaactta ttttgattta actactcatg gtgaaaagtt gacaaaggta
    18001 tcgaagatgt ttacagaaaa ttcttggtac atggatttgt ttgctaaaaa tatgattgga
    18061 gcctatgata caacaaaatt tggctctatt cctatgattt atgtaggttt acttccttta
    18121 ctcctttctt tactttactt tacaataaag gaaattcctc gaaggactag attagcatat
    18181 ggattttcac taatatttgt tattgctagt ttttatataa cacctctaga tttattttgg
    18241 caagggatgc atgctccaaa tatgtttctt catcgctact cttgggtact ttcagtactt
    18301 atctgtctac tagctgctga atgccttgag tatttagaca acatttcttg gaaaaagatt
    18361 ttaggggtca atttaatttt aataagtggt ttcataatca cgtttttatt taaaaagcac
    18421 tatcattacc ttaatcttga attacttctt ttaaccttaa cattcctctc agcttatatt
    18481 attctaacta ttagttttgt cagtaagcaa atccctaaat tagtttttta tccctttctt
    18541 attggttttg ttgtgttaga gatgacatta aacacctttt accaactaaa tagtcttaac
    18601 gatgagtggg tatttccatc taggcaagga tatgctaaat ataatcacag cattagcaaa
    18661 cttgtcagaa agactgagag aaataactct acattcttcc gtacagagcg ctggttagga
    18721 caaaccggca acgactctat gaaatataat tataatggta tttcacaatt ttcatctatt
    18781 agaaatcgtt cttcaagtca agtgcttgat agacttggct ttaaatcaga tggtacaaat
    18841 ttaaatttac gttatcaaaa taatactttg atcgcagata gtctatttgg tgtaaagtac
    18901 aaccttacag aatatccttt tgataaattt ggttttatca gaaaagctca agataaacaa
    18961 accattcttt acgaaaatca gtttgccagt caattagcta tactaacgaa tcaagtatat
    19021 caagataaac catttactgt gaatactttg gataatcaga caactctttt aaatcaatta
    19081 agtggtttga aagagacgta ttttgagcat cttattccaa a
//
LOCUS       DATRSG010000040        19038 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRSG010000040 DATRSG010000000
VERSION     DATRSG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19038
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.040"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            89..2722
                     /locus_tag="VBD89_001329"
     CDS             89..2722
                     /locus_tag="VBD89_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7135853.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SLLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            2822..3481
                     /locus_tag="VBD89_001330"
     CDS             2822..3481
                     /locus_tag="VBD89_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7135854.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            3490..3954
                     /locus_tag="VBD89_001331"
     CDS             3490..3954
                     /locus_tag="VBD89_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7135855.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            3954..4391
                     /gene="msrB"
                     /locus_tag="VBD89_001332"
     CDS             3954..4391
                     /gene="msrB"
                     /locus_tag="VBD89_001332"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO7135856.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            4540..7332
                     /locus_tag="VBD89_001333"
     CDS             4540..7332
                     /locus_tag="VBD89_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO7135857.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDNNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIMI
                     GLDEVRKLFSSRKNKR"
     gene            7332..8435
                     /locus_tag="VBD89_001334"
     CDS             7332..8435
                     /locus_tag="VBD89_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7135858.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYQAEISLE
                     FEKQFAAMVFTIPNIDYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFWGAYHDNSRETK"
     gene            complement(8553..9191)
                     /locus_tag="VBD89_001335"
     CDS             complement(8553..9191)
                     /locus_tag="VBD89_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO7135859.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
     gene            complement(9460..9809)
                     /gene="ssrA"
                     /locus_tag="VBD89_001336"
     tmRNA           complement(9460..9809)
                     /gene="ssrA"
                     /locus_tag="VBD89_001336"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9902..10513
                     /locus_tag="VBD89_001337"
     CDS             9902..10513
                     /locus_tag="VBD89_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO7135860.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            complement(10550..11332)
                     /locus_tag="VBD89_001338"
     CDS             complement(10550..11332)
                     /locus_tag="VBD89_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO7135861.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFYMLTHSIIKYKLNIQ"
     gene            complement(11344..12042)
                     /locus_tag="VBD89_001339"
     CDS             complement(11344..12042)
                     /locus_tag="VBD89_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135862.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            complement(12043..12411)
                     /locus_tag="VBD89_001340"
     CDS             complement(12043..12411)
                     /locus_tag="VBD89_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7135863.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            12556..15660
                     /locus_tag="VBD89_001341"
     CDS             12556..15660
                     /locus_tag="VBD89_001341"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO7135864.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKKRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            15741..16763
                     /gene="pfkA"
                     /locus_tag="VBD89_001342"
     CDS             15741..16763
                     /gene="pfkA"
                     /locus_tag="VBD89_001342"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO7135865.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            16812..18314
                     /gene="pyk"
                     /locus_tag="VBD89_001343"
     CDS             16812..18314
                     /gene="pyk"
                     /locus_tag="VBD89_001343"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO7135866.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            18485..>19038
                     /gene="lepB"
                     /locus_tag="VBD89_001344"
     CDS             18485..>19038
                     /gene="lepB"
                     /locus_tag="VBD89_001344"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7135867.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTAS"
BASE COUNT         6364 a         2980 c         3621 g         6073 t
ORIGIN      
        1 aaaaagatgg caaatagtat tgcaaaaaga aaaacatctg atataattaa ctagttaact
       61 aaaacgaaaa aacatttatg gaggactcat gaaaggtcaa aaaattattg ctctagctgg
      121 tcttgtcctg tcatgccatt ttgctcttac ggcatgtcat actcaagaac acaaaaattc
      181 ttatcatatt aaaacaaagc aggttgctaa gaaaaaagct aataaaaaga aagtctctgt
      241 taaggaaagt cataaaaaac gcaagggtgt tgcaggggtt gactttccaa ctgatgatgg
      301 ctttttactg actaaagatt cgaagatttt atctcataca gattctggaa tcgttgtagc
      361 acatggaaat cattctcatt ttatttttta ctctgattta aaaggttcaa aatttagcta
      421 tttaatccct aatgctaatg caaaaactaa taaaaatcaa gcggtaagaa actttaaagc
      481 aggggcggta gctgttaata cattaaatga tggctatgtt tttaatcccg cagatattgt
      541 ttcagaagat gctaatggct acgtggtcag acatggtgac catttccact atattccaaa
      601 agcaagttta tctcagcaaa agcaagtaca agcaagtaga gctgtttcac gtttaggaaa
      661 tcaaagtaat agccattaca gggttaatag ttctaaaatt gcagggcttc actacccaac
      721 aagtgatggt ttcttattta atggacgagg aataaaaggc actaccccaa caggtatttt
      781 agtagaacat cataatcatc tgcattttat tagttttgct gatttaagaa aaggggggtg
      841 gggatcaatt gctgaccgtt accaaccaca aaagaaagct gattctaaaa aacagtcacc
      901 atcaagtaaa aagccaagaa atgaaaatac tttacctaag gacatcaaag ataagctagc
      961 ttaccttgct cgtgaattac atttagatat ttcacgtatc agagtcctta aaacactaaa
     1021 tggtgaaatt ggatttgaat acccacatga tgatcataca catgttatta tggccaaaga
     1081 tattgatcta agtaagccaa ttccaaatcc acatcatgat gacgaagatc atcacaaagg
     1141 tcatcaccat gatgagtccg accataagca tgaagaacac gagcacacta aatcaaataa
     1201 attatctgat gaaaatcaaa agaaattaat ttatttagca gagaagcttg gtttaaatcc
     1261 taatcaaatt gaagtattaa cgtcagagga tggaagtatc atctttaagt atccacatga
     1321 tgaccactca cacactatag caagtaagga tatcgaaatt gggaagccta tcccagatgg
     1381 acaccacgat cactctcatg caaaagataa agttggtatg gcaactctaa aacaaattgg
     1441 ttttgatgat gagattattc aggatattct gcatgcagat gctccaacac cattcccatc
     1501 aaatgaaact aatcctgaaa aaatgcgtca gtggcttgca actgttacta agattaacat
     1561 tggacaaaga accaatccat tccagcgttt tggtctatca ttgatgccta atattgaagt
     1621 tctaggaatt gggtttactc cgattaatga tatgacgcca gttttgcaat tcaaaaaact
     1681 taaacagctt tggatgacaa atactggtat tacagattat tctttccttg ataagatgcc
     1741 actattagaa ggattggata tttcacaaaa cggtattaag gatttatctt tacttacaaa
     1801 atataaacaa ttaagtctta ttgctgcagc taataatggc attacttcgc taaaaccatt
     1861 ggctgaatta ccaaaccttc aattcttagt tttgagtcat aacaatatct ctgacttgac
     1921 accgttatca aacttgacaa aactccaaga attgcacctt gatcataata atgtgaagaa
     1981 tttaagtgca ctttctggta aaaaagattt gaaagttcta gatttatcaa acaataaatc
     2041 cgcagactta tcgactctaa aaacaactag tcttgagacg cttcttttaa atgagacgaa
     2101 tacaagcaat ctaagtttct taaagcaaaa tcctaaggta tctaatttaa caattaataa
     2161 tgcgaaatta tcatcattag atggtattga agagagcgat gaaattgtta aagtagaagc
     2221 tgaagggaac caaattaagt cactagtatt gaaaaataaa caaggttcgt taaaattctt
     2281 gaatgtgact aataatcagt taacatcact tgaaggtgtt aataattata cttcacttga
     2341 gaccttaagt gtttctagaa ataaacttaa atctttagat attaaaacac ctaacaaaac
     2401 agttacaaat cttgatttta gccataacaa tgttccaaca tcacaattga aattgaatga
     2461 aaaaaatatt cctgaagcag tagctaagaa ttttccagca gttgtagaag gttcaatggt
     2521 tggaaatggt agtcttgctg aaaaagcagc tatggcttct aaagaggaca aacaagtttc
     2581 agataatact aatcaccaaa aaaacactga aaaatctgct caagcgaatg ctgatagtaa
     2641 aaaagaaaac cctaaaacac atgatgaaca tcatgaccat gaagaaacag atcatgcaca
     2701 tgtaggtcat catcaccatt aatcttttac ggagctaata tataaaatcc ttcaagaatt
     2761 tcttggagga ttttgcattt atatgctaaa tcatttattt ttttagaaaa ttagctataa
     2821 tatgactatg gttaaaagag aagatgtgat tttaaatgca actgaaaata tggtttacca
     2881 taaattaaaa aatgatccaa gtggccatga ctggtttcat attgttagag taagaaatct
     2941 tgcagtagaa ttagcacaca aagaaggtgc taataccttt atttgtcaaa tggctgcact
     3001 tttgcatgat attattgatg acaaaatctg ccaagatagt aagcaagcgt cgtatgaatt
     3061 gactcaatgg ttatatagtc aagatcttgc catagcagaa gtagaacata tccttgatat
     3121 tcttgaaaat atttcattca aagcaggaac tggattgact atgaaaacat tggaaggtca
     3181 aattgttcaa gatgcagatc gcctagatgc tatgggggct attggtattg caagaacgat
     3241 ggcttattca gggagtaaag gacgtttaat tcatgaccct aacttaaaac cacgagaaaa
     3301 cctcacttta gaagagtatc gtaatggtca ggatacagct atcatgcatt tttacgaaaa
     3361 attattaaag ttaaaagacc ttatgaatac aaaacaaggg aaaatgcttg ctcaaaaacg
     3421 tcatgatttt ttagaactat atttagccga attttatgct gaatggaatg gtaaaggtta
     3481 ggagtagata tgattcgtag agcgaaagaa aaagatttgc ctgctattgc agagctattg
     3541 aaacaaattt tagtgttaca tcatggggtt cgcccagata tttttcatac aagaggaagt
     3601 aagttttcta aagaacaatt gaaagaaatg ctgatggacg aatcaaggcc tatctttgtt
     3661 tatgaatcag acgagggtaa agttgttgcc cacttgtttc ttcaattaca agaaaaacgt
     3721 gacttacctc gaaagtcctt taaaactctc tatatagatg atttgtgcat tgatgaggaa
     3781 gtaagaggtc agcaaattgg acaaaagttg atggattttg caagacaata tgctaagaaa
     3841 catgggtgtt ataatataac tttaaatgtt tggaatgata atcagagagc ggtctctttt
     3901 tatgaaaaat taggttttaa acctcaacaa actcaaatgg aggagatact ataatgaaag
     3961 aaacacaaga agaattacgt cagcgtatcg gacatacagc ttatcaagta acccaaaatt
     4021 cagctacgga acatgctttt actgggaagt atgatgattt ttttgaagag gggatatatg
     4081 tagatatcgt cagtggagag gttttattct cttctttaga taaatttcaa tctggttgtg
     4141 gttggccagc attttcaaaa ccaattgaga atcgaatggt gacaaatcat caagatcact
     4201 ctcatggaat gcatcgtatt gaggttagga gtcgtcaagc tgactctcat ttagggcatg
     4261 tatttaatga tggaccggtt gatgcaggtg gcttacgcta ttgtatcaat tcagctgcct
     4321 tagattttat tccttatgac cagatggcaa agagaggcta tggggactac ttatcacttt
     4381 ttgatgaata agagacgact taagggtata tcttaagtcg ttttttatgt atggtaaaaa
     4441 gaattggtgg caggtgtatt cttcatcagt ttattttctg aaaaaagagt ataatatagg
     4501 tatcaaaaat gaaaacgttt tagagaagga gactagaata tggcttctac aaataatgtt
     4561 tcagatacaa acacacgttt tgctaaagag gaattagaag aggttttcga agaattaggg
     4621 acaactcaag gtggtttatc agatgaagag gttgctgtac gtcagaaaaa atatggttta
     4681 aacctgttat ctgaagtaaa gcaagaatca attattttat tgtttctgaa aaattttact
     4741 agtcttatgg caatactatt atgggttgga ggatttgtgg ctattgtttc taatagctta
     4801 gagctaggtc ttgccatttg gatggttaat gtgattaatg gtatttttag tttcattcag
     4861 gaataccgtg caagccaagc aacacaagct ctcaagaaaa tgttaccatc ttattcacgt
     4921 gttcttagaa aaagctcaga agaaaaggta ttatcagagc aactagtccc aggagatatt
     4981 gttcttattg aagaaggaga ttgtatttca gcagatggtc gcttgattaa aacaacagat
     5041 ttgcaagtaa accaatcagc tttgacaggg gaatcaaatc ctatttataa agataacaat
     5101 gttgaaaact accagtctaa aacattgatt gaatgtgata atatggtttt cgcaggaacg
     5161 acagtctcca gtggtagcgc aacaatggtt gttacggcaa ttggaatgca aacccaattt
     5221 ggtcaaatcg ctgatttaac acaagggatg aaatcagaaa aaagccctct tcaaagagaa
     5281 cttgatcggt tgaccaaaca gatttctatc atttcaatta ctgtaggaat tatctttttc
     5341 ttagcagcga ctttttttgt taaggagcca gtgtcaaaat catttatctt tgccttgggg
     5401 atgatcgttg catttatccc agaaggttta ctacctaccg ttacactttc tctagctatg
     5461 gcagtccaac gaatggctaa agaacacgca cttgtcaaaa aactttcttc agttgagact
     5521 cttggagcaa cctcagttat ctgttctgat aagactggta cattaacgca aaatgaaatg
     5581 accgtcaatc atttgtggca aaatggaaag agttaccaag tgacaggact gggttatgct
     5641 ccagaaggac agattctttt tgaaggtgac aatatttgct ttggtaatag tgatagagga
     5701 gacttagaaa aacttattcg ttttgctcat ctctgtagca acgctcaagt attaccacca
     5761 aatgatgatc gttcaactta tactgtactt ggagatccga cagaagcttg tttgaatgtt
     5821 ttattggaaa aatcagggat aaacatacaa gaaaatcgta agtttgcacc acgtttgaaa
     5881 gagttacctt ttgattcggt tagaaaacgc atgacaacta ttcatagctt aggaggcgat
     5941 gaaaaggata aaaaaatttc aatcacaaaa ggtgcgccga aagaaatact tgatttatca
     6001 gattatgtct tatctgatgg caaagttatc cccttaaata aggaagaacg taataaaata
     6061 cagttggcta atgatacatt tgctaaagat ggacttcgcg tcttagctgt ttcatactgc
     6121 gatattgaag gcttttcaaa agaacaatgg acgcaagaaa atttggaaca acacatggtg
     6181 tttatagggt tgatagcaat gagtgatcca cctcgtgaag gcgttcgtga agctattgat
     6241 aaatgtcatg cagcaagcat tcgtattatt atggtgactg gagattacgg attaacggca
     6301 ctgagtatcg ctaaaaatat tggtattatt cgtaacgatg atgctaaagt aatttcaggt
     6361 ttagagttat cagaaatgac ggatagccaa ttgaaaaaag aattgtcagg tgaagtcgtt
     6421 tttgctcgtg tagcaccaga acaaaaatat cgagtagtaa cgatattaca ggagatgggt
     6481 gaagtagtag cagttacagg agacggggtc aatgatgcgc ctgcgctaaa aaaatccgat
     6541 attggtgtag ccatgggtgt taccggtact gatgttgcta aagaatcagc tgatatgatt
     6601 ctgacagatg atcattttgc ttctattgta catgccgtgg aagaagggcg ggctgtttat
     6661 caaaacatta agaaattctt gacgtatatt tttaatagta atactcctga ggcggttccg
     6721 tcggcattct tccttttttc taaaggtttt attcctttac cattgactgt aatgcaaata
     6781 cttgctgttg atttgggaac cgatatgctt cccgctttag gtctaggcgt ggaaccacca
     6841 gaaacagatg ttatgaatcg tcctccaaga cggctaacag atcgcttatt ggacaaaggg
     6901 ctacttataa aatctttcct ttggtacggc actattgaat ccgtattagc tatgggtggt
     6961 tttttctggg ctcattactt acgttatggt aattttactt ttttcgtcgc aaacggcata
     7021 ccgtacagag aagcgacaac tatgacactt ggagctatta tctttagcca gataggtatg
     7081 gttatgaata gcagaacgag ctatcaatct attaaaacac tatctatttt tggtaataaa
     7141 ttaattaatt ttggtataat aatggagatt ttagcatttt tagtcttagt atacgtgcca
     7201 ctattccata acttattcaa cacagcaagc ctaggtttat cacattggct ctatttaata
     7261 agctgcccgt ttatcatgat aggtttagat gaagttcgga aattattcag cagtagaaag
     7321 aataaacggt gatgtcaaat ataataactt atttaaaaaa taattcaaac ttaacttttg
     7381 atgaattagc attaaacgat gttgacattc tttgtttaaa tgaatttggc tatatttctt
     7441 ttgaaaaact tataaacact acagaaatga agtcagtttt agtctgtgag ttatatcatg
     7501 agtaccttca aactatggct aaatcatata gctttatgtt cacgtcacaa cgacatgatt
     7561 tatgtcagtt aatgatgacc tcgaaacgtt ttaaaaactt aactttatct tattatcaag
     7621 ctgagattag tttagaattt gaaaaacaat ttgcagctat ggtttttact attcctaata
     7681 ttgattatca tcaggttgta tttcgtggta ctgatgccaa ccttattggt tggaaagaag
     7741 attttaaatt gacgtatatg agagaaatat cggctcatcg ttcagcgatt aaatacttaa
     7801 atacaatact gccttatttt gataaagtag ttctctcagg tcattctaag ggtggtaatt
     7861 tagccttgta cgctgcaatg tttacaaagc cagatttaaa agcaaaaatt gatttgattt
     7921 ggcttattga ctccccagga ctacaaaaaa ctttgttacc tactacagag tataaaacta
     7981 ctaaacaaaa gtgcattcgc ttactaccag aagaatctat tgttggtatg atgctttata
     8041 gtgatataga gccgctaatt attagtagca atgcgcgtgg aattctgcaa cacgatgtta
     8101 caacttggga aattcaagaa ccagctattt taaagactgg agcaggacta tcgctaaaaa
     8161 gtatttgttt tgaaaagaca tttcaacagt ggatggctga attaaaaagt caagaaagaa
     8221 aattattttt tgatttatta tttgatagct tcctatcaag tggcgtatct agtttggatg
     8281 acttcaactt ggcttccaga gcaaaaatga tgaaagcttt tcatagcttt agagaactag
     8341 atgatgataa aaaaaggtta tttaacaaat ctttaaaatt attagtaacc atattttggg
     8401 gagcttatca tgataatagc agagagacta agtagctttc ctagaattgc attagtctaa
     8461 acatgtgcta aataaactag caaaatagca tactaaaata ctactcatat tatgctgagt
     8521 agtattttag tgtgctattt gatgaagtca ttctagagtt tatgattttt gttttcagaa
     8581 atacttttta gagtattgaa gaattccctc ttatctgaaa attcgctaat atgttcctta
     8641 atagcttcat ctgaccaatt ccaccactga gatgcattta ataatgttat ttcttcctct
     8701 gaaaagcgat atttgataat tttagcaggt gttccagcga caacagcata gtcaggaatg
     8761 tctttggtaa taactgaacc tgcttcaata attgctccat tacctattgt aacagatgga
     8821 agtatagtga cattagttcc tatccagaca tcgttgccaa taataacttt tccatttttc
     8881 ttttcatcta ttaagtccgc cctatcctca tttataaatc cccttgactt atagtaaata
     8941 aaagggttag tggtaatgtg atcggttgga tgattcatac cagtaatagt aacgttctgg
     9001 gctattgagc aaaatgctcc aatttctttt aagttaacgc cttcgtaaaa gaattgttga
     9061 tatccatatg tgtactttcc taccgagaat ccatatttag ggtaactggc accagtactc
     9121 atatctgtcc tcataggatc atttttaagc tctaaacgtt tctctaaatg tttttttaaa
     9181 atactagcca taactagcct ccaatcgtag gctaaataga ttataacatt ttttcctcta
     9241 aaaaccttat cttttttaat aaattctata aattagaatg ataaagaact tattctcgga
     9301 tattgacaaa gagtgagcta atgaggtggc ctaattcaat gttagagtct gttccaagta
     9361 tttttttcct aagatagggg caatgtatga gcaaagaaac aaaataaaaa ccttgtaata
     9421 acaaggtttt aagtatgtat agtaagttat gataaaatga tatggagccg gtgggagtcg
     9481 aacccacgtc caaacatctg ctaatatatt tgtctacaac tataggttat gtattgattt
     9541 aactaggtga tgacacataa ctcaagccct cacatagcga gtctataaat ctctttttaa
     9601 ctaactagac agtagccaaa cgtagtttgc tgataaataa gaccttctag caaaacgcaa
     9661 acaatcttgt gaagatcacg caggctgttt tttaggcagc taatgcgtaa gaatttgtat
     9721 tttttgcagt tatatttaac tggcatttta catctgccga tgagtcgcaa aatatacctc
     9781 ataatgcctg tcgaatccgt aacgacccca aaagtagaat aaagcataaa aacttaccaa
     9841 tatagtataa caaatactat ttaaaatagc aaaaatttaa cagttgtgtt atgattaaag
     9901 catgaatatg aaattatcaa aacgttaccg cttttggcag aaggtcatta aggctttggg
     9961 tgtcttagcg ttaatagcga cccttgtctt agttgtttat ttgtacaaac ttggtatttt
    10021 gaatgatagc aatgaattaa aagacctagt acacaagtat gaattttggg gtccaatgat
    10081 ttttattgtg gctcaaattg tacagattgt ttttccagta attcctggag gtgttacaac
    10141 agtagcaggt ttcttaattt ttggtcctac cttaggcttt atttataact atattgggat
    10201 tatcatcgga agtgtcatcc ttttttggtt agtcaaattt tatggacgaa aattcgtttt
    10261 gctatttatg gatcagaaga catttgataa atatgagtct aagctagaaa catccggata
    10321 tgaaaagttt tttatctttt gtatggcatc tcccatttca ccagcggaca tcatggtgat
    10381 gattaccggt ctatctaata tgtctattaa gcgctttgtg acaatcatta tgataacgaa
    10441 acctatttct atcatcggtt atagctattt gtggatttat ggcggtgata ttttgaaaaa
    10501 ttttttgaat taaaaataaa aaaaggttgg agatatatct ccaatctttt tattgtatat
    10561 taagtttgta cttaataata gagtgtgtca acatatagaa tatagcaatt tcaacaatgc
    10621 taacaagtat attgtagagc aatacaggtt gagagaatgt tgcatttgca tgagagttga
    10681 ataaatcaat atgaaaaata ctatctatta ttaatgttaa gaaagtaatt aagagtgata
    10741 ttccaaaata agaaacaatt cccatcaata cacgtttgtt tgtaaataat tgccctacag
    10801 cgatagagag ataaatcagt aatgtccctg caacgtacga tagaaagaaa taggcgataa
    10861 aaagtggagc gttcgaagat gaaataattt tatagatttc tggaaaagcg acaacgaact
    10921 gaccaatacc tactaaaggt aatatgacca atatgattcc aataatgaca ataaaaatat
    10981 taaaaatact ccaaagaagc gaagctagta gctttgaaca aataatctga ttcgtagtta
    11041 ctgggagcgt taaggttaga tatccttgac ggccgaagat attgctataa aaacgtttga
    11101 taataatagc aagggtggat aacagagatc ctgaaataac catcgctaac aataaaacga
    11161 gtattatagt gaatatttgg gcgctagtac tattagtatc agtagaaaaa ttgccgccaa
    11221 gtgcctttaa taccagtcct aaaataatag atactaataa aaccgcagcg ttgagtgtta
    11281 gataccactt accaactgat tttaattcat attttaataa ttttccaaac atatcaatct
    11341 cctttatttc ttgaatcttt cacgaaagag agcatcaatc gaacagttgt gctcttctct
    11401 taagtcatca gcattgcctt gtaggtcaat ttctccttct ttaaggaaga taacttcatc
    11461 aaggataggt tcaatgtcag aaattaagtg agtcgaaatt aaaacagaag catcattaga
    11521 gtagttgcta atgattgttt ttaaaatgta gtcacgcgct gcagggtcta caccaccaat
    11581 aggttcatca aggatgtaga gcctagcttt acgactcata acaagtatta gttgaacttt
    11641 ctccttgttc ccttttgata ggttttttag acgttcctta ggatttaggt taagatcctc
    11701 aaatagttga taagctacct ttgaattaaa atcgttatag aaatcttcaa ataatgtaac
    11761 gacgtctttg attttcatat tttcttgtaa atatgaagta tcagggagat atgaaataat
    11821 ttttttggtt tcaacagacg gtctgtaacc atcgataaca atatctccct tatctggctg
    11881 aagtaatcca tttatcattt tgatgattgt tgtttttccg ctaccatttg gacctaataa
    11941 acctataatc tttcctgttg gaatgcttac agtcacatca ttaacagcaa tatgtttgtg
    12001 atatttcttt gtaacatgat gaagttgtaa taactgtgtc atttaattaa tctcctttat
    12061 aaaatgatcg agagcaaccg taatttcatc taatttatat cctatttttt tcatgttgtt
    12121 gacaaaagtt gctaactctg cttttgcaac ttgttgacga attttaccaa ttagtaagtt
    12181 gtcttctgta acaaagcgtc cggaagtacg ttgtgagaat accattcctt cacgttccaa
    12241 ttcagtgaag gcacgttgca tggtatttgg gttaacacca gcttcttgag ctaactctct
    12301 cacggtaggc aattggtcac cactttttat ttcttgacta acaatttgca tctttatatg
    12361 ttcagcaatt tgtgaataaa taggtgattt ttcattgaat tcccaagcca tagataactc
    12421 ctttcataat cttgtacttt ttgtactata aaaatagtac aaaaaaatat ttgaaatgtc
    12481 aaggaattat cattgagact ttaatcgttt attgtttcat agtataatag taaaaaatcg
    12541 taaaaagggg ctactatgtt tgcacaactt gatacaagaa ccgtttattc ttttatggat
    12601 agcttggttg atttaaagac atatgtctct aaatcgaaat cattaggata tcaaactatt
    12661 ggtatcttgg accacagtaa tctttatgca gcatatcatt ttattcaaga ggcgcaaaag
    12721 gcaaacctaa gaccaatagt tggatttagc tttgatatag tggttgaaaa tagatcaata
    12781 gaagtttact gtattgcgat aaatacagtt ggttataaga atctcttgaa gttgtcaacg
    12841 gctcaaatgt ctgagaagat gtctctaaat ttattgacag aacatcttga aggggtgcaa
    12901 ttgattcttc cctatcaaga tgctcttgat cagctcaatt taccctttga ttatgttatt
    12961 ggtgttaatt tgacctctcc gcagattcca tataccaaac ctattatagc catagatact
    13021 gttcgatatt ttcaaaaaaa tgatattgag acattacaga tgcttcatgc tataagagat
    13081 aatgtcctgt taaaagatgc acaatatgcg agcaaaaatc aagaattaaa accttgtcaa
    13141 gaaatgactc tagcattcaa gaaacgtttt cctgaagctt tagctaacct agaatctttg
    13201 gtagagaatg ttgtttatca ctttgattct gactttaagt tacctatttt taatagagaa
    13261 attcctgctg gagaagaatt aagaacctta actcaaaata acttaaaaag taaaggttta
    13321 tggagcgatg cttatcaaga gcgtctcgaa aaagaattaa caatcattca caaaatgggc
    13381 tttgatgact atttccttat tgtgtgggat ttattaagat ttggtcgtag taagggctac
    13441 tatatgggaa tgggacgtgg ttcagcagcg ggaagtttag tctcatatgc tttaaatatt
    13501 accggtatcg atcctgtcaa acataattta atttttgaac gttttttaaa tgaagagcgt
    13561 tattcgatgc cagatattga tattgatctt ccggatattt atcgtggaga attcttgcgc
    13621 tatgtacgta atcgatatgg ttcaatgcat tctgcacaga ttgtaacctt ctcaactttt
    13681 ggtgcgaaac aggctattcg agatgttttt aaacgctttg gtgcatcaga gtatgagtta
    13741 acaaacataa ctaaaaaaat tcatttcaga gataatttga ccagtgttta taaccgcaac
    13801 ttagctttca gacaaataat agatagtaaa attgaatacc aaaaagccta tgatattgct
    13861 aagcgtattg aaggaaatcc tagacagact tctattcatg ccgctggggt agtaatgagt
    13921 gacgatttgt taacagatca tattccttta aaaaacggtg aggatatgat gataacgcaa
    13981 tatgatgcta gttctgttga ggctaatggt cttctaaaaa tggactttct aggtcttcga
    14041 aatttgacat ttgttcagaa aatgaaggag aaagtcgata aggactacgg tatctctata
    14101 caattagaag ctattgattt agaagataaa gagactctaa aactatttgc ggctggtcaa
    14161 acaaagggga tttttcaatt tgagcaaagt ggagctatta atttgttgcg acgtattagg
    14221 ccagagtgtt ttgaagatgt ggtagctact accagcttga ataggcctgg tgcaagtgat
    14281 tatactgaga attttataaa ccgtcgtttt ggtaaagaaa aaatcgattt agtagatcct
    14341 gtcatagctc caattttaca acctacctat ggcataatgc tctatcaaga acaggttatg
    14401 cagattgccc agacctacgc aggttttacg ctaggcaagt ctgatttgct cagacgtgct
    14461 atgtctaaga aaaattctaa ggagatgcaa aagatgtctc aatccttctt agagggtgca
    14521 gtatctaagg gacatcgtca agaagatgct cgacttatat ttgaacgcat ggctaaattt
    14581 gcaggttatg ggtttaatcg cagccatgcc tttgcttatt cggctttagc ttttcaatta
    14641 gcctatttca aagcccatta ttccgatgtc ttttatgaca tcatgataaa ttattcaaat
    14701 agtgattatc taatcgatgc tattgatttt ggttttgtta ttgaaaaacc ttcaattaat
    14761 actatttcat acagagaccg catctataaa aagaaaattt atttaggttt aaaaaatatt
    14821 aaggctgttc ctaacgactt ggcttattgg atttctaaaa atcaaccatt tcagagtatt
    14881 gaagattttc taatgagact gccacagcag tttcaaaaaa gtggttttat ttctccgcta
    14941 attgctattg gagcatttga cgaatttgat aacaatagac ggaaaatcac ctctaatctt
    15001 gattctttat ttacatttgt aaatgaacta ggtagtttat ttgcagatac ctcatatcat
    15061 tggttagaag tagaagattt ctctaattct gagaaatatg aaatggaaca agatatctta
    15121 ggagtaggaa tcagtccgca ccctttagtg gggatttcac aaaaagctag ccgtccgttt
    15181 ataccaatta gtcaagtcca agaaaatagc gaagctacta ttttagttca gctaaagcag
    15241 gtcaaaatca ttaggacaaa aagttctgga caacaaatgg ctttcttgac tgttatggat
    15301 attaattcaa aaatggatat tactgttttt cctgaaactt tcaatattgt aagagatgat
    15361 ttacaagaag ggaaatatta ttacttgcat ggaaaaattc agaagcgaga tgagcgatta
    15421 cagatggttt taaacggtgt tcaagaagca acggaggaac gtttttggat acttctaaaa
    15481 aatcatgata acgacaaaaa aatatcggaa atattgtcca aatataaagg gcatattcct
    15541 gtctatttgc attatgaaac aactaaagaa actatccaaa gcaaagtaca tttggttaga
    15601 aaagatagtg gcttagccct tgatttatct gaatttgttg tgaaaacggt ttatcaataa
    15661 aattgcatat aaagtgcaga aatttacaaa ttaatatgct ataattaaat cgttaaatta
    15721 ataaaaggag taccaattaa atgaaacgta ttgctgtttt aactagtggt ggtgacgccc
    15781 ctggtatgaa cgctgctatc cgtgcagttg ttcgtaaagc aatttctgaa ggtatggaag
    15841 tttacggcat caaccaaggt tactatggta tggtgacagg ggatattttc cctttggatg
    15901 ctaattctgt tggggatact atcaaccgtg gaggaacgtt tttacgttca gcccgttatc
    15961 ctgaatttgc tgaacttgaa ggtcagctta aagggattga acagcttaaa aaacacggta
    16021 ttgaaggtgt agtagttatc ggtggtgatg gttcttatca tggtgctatg cgtctaactg
    16081 agcacggttt cccagctgtt ggtttgccgg gtacaattga taacgatatc gttggcactg
    16141 actatactat tggttttgac acagcagttg cgacagcagt tgagaatctt gaccgtcttc
    16201 gtgatacatc agcaagtcat aaccgtactt ttgttgttga ggttatggga agaaatgcag
    16261 gagatatcgc tctttggtca ggtatcgctg caggtgcaga tcaaattatt gttcctgaag
    16321 aagagttcaa tattgatgaa gttgtctcaa atgttagagc tggctatgca gctggtaaac
    16381 atcaccaaat catcgttctt gcagaaggtg ttatgagtgg tgatgagttt gcaaaaacaa
    16441 tgaaagcagc aggagacgat agcgatcttc gtgtgacgaa tttaggacat ctgctccgtg
    16501 gtggtagtcc gacggctcgt gatcgtgtct tagcatctcg tatgggagcg tacgctgttc
    16561 aattgttgaa agaaggtcgt ggtggtttag ccgttggtgt ccacaacgaa gaaatggttg
    16621 aaagtccaat tttaggttta gcagaagaag gtgctttgtt cagcttgact gatgaaggaa
    16681 aaatcgttgt taataatccg catgaagcgg atcttcgctt ggcagcactt aatcgtgacc
    16741 ttgccaacca aagtagtaaa taagaatttg tcaatttgac actttaaata aaaaggagct
    16801 cataaattat tatgaataaa cgcgtaaaaa tcgttgcaac acttggtcct gcggtagaat
    16861 tccgtggtgg taagaagttt ggtgagtctg gatactgggg tgaaagcctt gacgtagaag
    16921 cttcagcaga aaaaattgct caattgatta aagaaggtgc taacgttttc cgtttcaact
    16981 tctcacatgg agatcatgct gagcaaggag ctcgtatggc tactgttcgt aaagcagaag
    17041 agattgcagg acaaaaagtt ggcttcctcc ttgatactaa aggacctgaa attcgtacag
    17101 aactttttga agatggtgca gatttccatt catatacaac aggtacaaaa ttacgtgttg
    17161 ctactaagca aggtatcaaa tcaactccag aagtgattgc attgaatgtt gctggtggac
    17221 ttgacatctt tgatgacgtt gaagttggta agcaaatcct tgttgatgat ggtaaactag
    17281 gtcttactgt gtttgcaaaa gataaagaca ctcgtgaatt tgaagtagtt gttgagaatg
    17341 atggccttat tggtaaacaa aaaggtgtaa acatccctta tactaaaatt cctttcccag
    17401 cacttgcaga acgcgataat gctgatatcc gttttggact tgagcaagga cttaacttta
    17461 ttgctatctc atttgtacgt actgctaaag atgttaatga agttcgtgct atttgtgaag
    17521 aaactggcaa tggacacgtt aagttgtttg ctaaaattga aaatcaacaa ggtatcgata
    17581 atattgatga gattatcgaa gcagcagatg gtattatgat tgctcgtggt gatatgggta
    17641 tcgaagttcc atttgaaatg gttccagttt accaaaaaat gatcattact aaagttaatg
    17701 cagctggtaa agcagttatt acagcaacaa atatgcttga aacaatgact gataaaccac
    17761 gtgcgactcg ttcagaagta tctgatgtct tcaatgctgt tattgatggt actgatgcta
    17821 caatgctttc aggtgagtca gctaatggta aatacccagt tgagtcagtt cgtacaatgg
    17881 ctactattga taaaaatgct caaacattac tcaatgagta tggtcgctta gactcatctg
    17941 cattcccacg taataacaaa actgatgtta ttgcatctgc ggttaaagat gcaacacact
    18001 caatggatat caaacttgtt gtaacaatta ctgaaacagg taatacagct cgtgccattt
    18061 ctaagttccg tccagatgca gacattttgg ctgttacatt tgatgaaaaa gtacaacgtt
    18121 cattgatgat taactggggt gttatccctg tccttgcaga caaaccagca tctacagatg
    18181 atatgtttga ggttgcagaa cgtgtagcac ttgaagcagg acttgttgaa tcaggcgata
    18241 atatcgttat cgttgcaggt gttcctgtag gtacaggtgg aactaacaca atgcgtgttc
    18301 gtactgttaa ataatttttg aattcaaacc tattatttaa gaatacctta aataatgggt
    18361 tttttatatg ttttgctttt tattattcga ttattgtgac tagaatagga tatgatatta
    18421 ataattaccc tccttttttt atctatgaaa acatgatata atggaaaaaa gattaggagt
    18481 agttatggtt aaacgcgatt ttattagaaa tattatttta gcattgattg ctgtattgat
    18541 tttgattctc ttgagatatt ttgtttttgc aacctttaaa gtacataagg atgctacaaa
    18601 ttcttatttt agcaacggag atgttgttgt tgttaatcgt aataggacgc ctaagtacaa
    18661 agactttatt gtctataagg ttggaaagat tttctatatt agtcgtgtta ttggtgagcc
    18721 aaatcaaaaa gtgagagtaa tggatgatat tttatatttg aatgatgttt tcaaggatga
    18781 gccttacatt gaaaaaatga aaaatgctta ttctgaaaag aaagacggtc aaatgccctt
    18841 tacttccgac ttctcagttg aaacattaac acgaaataaa gaaagcagag ttcctaaggg
    18901 ttcttatctt gtgttaaatg ataatcgaca aaataaaaat gatagtcgaa aatttggatt
    18961 aatcaaagag aaagatattc gtggtgttat tacctttaaa gtataccctc taagtgaatt
    19021 tggttttaca gcatcaga
//
LOCUS       DATRSG010000041        18529 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRSG010000041 DATRSG010000000
VERSION     DATRSG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18529
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.041"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            50..346
                     /locus_tag="VBD89_001345"
     CDS             50..346
                     /locus_tag="VBD89_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO7135868.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(432..971)
                     /locus_tag="VBD89_001346"
     CDS             complement(432..971)
                     /locus_tag="VBD89_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO7135869.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            1110..3428
                     /locus_tag="VBD89_001347"
     CDS             1110..3428
                     /locus_tag="VBD89_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO7135870.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQNGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTVGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTTALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            3495..3851
                     /locus_tag="VBD89_001348"
     CDS             3495..3851
                     /locus_tag="VBD89_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO7135871.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAIFLINVLLVAVYGALTVDKKIL
                     LKQGGLPILALLTFLF"
     gene            3985..4728
                     /locus_tag="VBD89_001349"
     CDS             3985..4728
                     /locus_tag="VBD89_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7135872.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            4712..7252
                     /gene="mprF"
                     /locus_tag="VBD89_001350"
     CDS             4712..7252
                     /gene="mprF"
                     /locus_tag="VBD89_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO7135873.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGIVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(7331..8845)
                     /locus_tag="VBD89_001351"
     CDS             complement(7331..8845)
                     /locus_tag="VBD89_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO7135874.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(8848..9570)
                     /locus_tag="VBD89_001352"
     CDS             complement(8848..9570)
                     /locus_tag="VBD89_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7135875.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            complement(9792..10625)
                     /locus_tag="VBD89_001353"
     CDS             complement(9792..10625)
                     /locus_tag="VBD89_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO7135876.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            complement(10622..11917)
                     /locus_tag="VBD89_001354"
     CDS             complement(10622..11917)
                     /locus_tag="VBD89_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO7135877.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYTRSIIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDKQK
                     KGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHV
                     RILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            12020..13018
                     /gene="argF"
                     /locus_tag="VBD89_001355"
     CDS             12020..13018
                     /gene="argF"
                     /locus_tag="VBD89_001355"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7135878.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            13030..13956
                     /gene="arcC"
                     /locus_tag="VBD89_001356"
     CDS             13030..13956
                     /gene="arcC"
                     /locus_tag="VBD89_001356"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7135879.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGIALITSLNGLEDALDGKLGTRITK"
     gene            14050..15603
                     /locus_tag="VBD89_001357"
     CDS             14050..15603
                     /locus_tag="VBD89_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO7135880.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(15713..17077)
                     /locus_tag="VBD89_001358"
     CDS             complement(15713..17077)
                     /locus_tag="VBD89_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7135881.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(17061..17732)
                     /locus_tag="VBD89_001359"
     CDS             complement(17061..17732)
                     /locus_tag="VBD89_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7135882.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            complement(17817..18488)
                     /locus_tag="VBD89_001360"
     CDS             complement(17817..18488)
                     /locus_tag="VBD89_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7135883.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
BASE COUNT         5844 a         2850 c         3462 g         6373 t
ORIGIN      
        1 gaatgataac ttatctcgtc gaatcttata taactaagga gattttatta tgaatctaaa
       61 tgatagatta aaaatagaag aaatggaaga gaagtatgat agcttcaaac ctagaatcaa
      121 tgcattagta gaagctatag atgattttca aaagcactat gaagattacg tgaaattaag
      181 agaattttat ggtagcgaag actggtttag actatcagaa caaacagaaa ataatctcaa
      241 atgcggtgta ctgagtgagg atcagctctt tgattttata ggagaacata acgagcttgt
      301 aggccaattt ttagatatgt cttctcaaat gtatcgtcat ttataatgtc agtccttatt
      361 agtgttgtta tcctataatg aggataaaat aaacctaact aaaaatgacc agcctataag
      421 gaaggctggt tttaagtttg aggtaataaa ctgagtagca cttgtgtaat ttgttgaggg
      481 ctttcttttt ttcctcgtgt tatccacatt tgatagactc caaaaatagc atgactgaga
      541 tagacactat aatacaaacg gttgatatca gactttgttg catctggatt gaccacaggt
      601 aattctttag aaagcattaa ttgtaattta ttaaggatgt aggtctgaat ttcttttgtt
      661 ccattttggg ttaagagagc agcaaataat gattctctat ctaagaattg aaaaatttcg
      721 agcaaggcat cttctttttt aaattgattg cgatcaaaaa tatattcgac tttattgaag
      781 agtgactgtt ggtattgatc aatcatttca tatttatctt tataatgagt ataaaaacta
      841 gaacgactga ttccagctgt tttagttaga ttaatcgttg aaatttcatc gaagctctga
      901 tcttttaata acgtaatcat agctgcttca atagctcttt ttgttttttc tctcctcgta
      961 tcgtttacca tattaattac cccttaatat ttttgtacat ttttaaacac agtgtccaaa
     1021 aacgagattt ttatcttgaa attatctatc atcactgtat aatatatttt atcaaaaaaa
     1081 tgaacaatat gtccaaaata ggagaaaaaa tgttagacga attaaaagca atcataaaaa
     1141 gcccaaaatt atggattacg atggctgggg ttgcactgat tccaacgcta tataatgtta
     1201 tctttctaag ctcaatgtgg gatccttatg ggaatactaa aaatttacct gtggcagtcg
     1261 ttaaccaaga taaatcggct aaattaaatg gaaaaactat ttcaattggc aaagatatgg
     1321 aggacaattt gtctaaaaat gatagcttag atttccattt tacaacagct aaaagagcag
     1381 aaaaagaatt agaaaagggt cactattata tggttattac ctttcctaaa gatctttcta
     1441 gaaaagcgac aactctcatg acagaaaaac cagaaaggtt aaatattact tataaaacta
     1501 ctaaggggcg tagttttgta gcttcaaaaa tgagtgagac agcagctaat aaactaaaag
     1561 atgaggtagc agaatctatt acagggactt atactgaatc agtttttaaa aatatgggtt
     1621 ctatgaaaac aggtatcaat aaagctgctg atgggagtca agaattatta aatggttcta
     1681 ataagctaca gaatgggagc caaaccttga caagtaattt agatgtttta gcaagctcta
     1741 gtcaaacttt ttctggagga gctaataaac taaatagtgg aataaacctt tatacagatg
     1801 gtgtgggaac cctatctaac gggttagaaa cattatcaga tggtgttact gcttacacaa
     1861 caggtgtgca taagttgagt gaaggtagtc aaaaattaga tgataagtct caagcattgg
     1921 tggaaggatc agaaaagttg acggttggtt tacaacaatt gtcacaagca actcaactaa
     1981 aaccagagca agaacgtact ttgcagaacc tttcagatgg attaaagaat ctaaatcaga
     2041 ttattacaaa tttacaatcg acagcaacta ctgatagcga tactaatagt aagttattta
     2101 attttttatc aactattgaa tcaagtacaa cggctttgat gaatacggct gctgctgata
     2161 aacaaaagca aatgactgcg gtacaatcaa cgtcagcttt taaaagttta acccctgaac
     2221 aacaatctca aattacctca gcggttactg gaacacctac ttcagctgag acaattgcag
     2281 caaatatttc ttcaaatata gaaaatatga aaactgtgct tagtgaagca tctagttcag
     2341 ccccttctaa taataactca caaaattttc aaactttgtc aggaacagct aacaatttag
     2401 tattaaaagc tatctcagat ttagataaaa ttcaaaaatt gccaacggct actaagcagt
     2461 tatatcaagg tagtcaaacc ttgacaaaag gaatcacgga ttatacgaat gctgtagggc
     2521 aattacgcaa aggtgcagaa actttagata gtaaatccaa tcaattgatt tcaggaacac
     2581 aaaaagcaag tcagggagct caaactttag atagtaaatc ggatcaattg agagatggag
     2641 cggggcaatt agctagtggt tctgatagaa ttgcagatgg ttctaataag cttgctgggg
     2701 gaggacatca gttgacagat gggttgacag agttgtcagg tggtgtttct caattatcaa
     2761 gttctttggg taaagctggg gaccagttgt ccatggtttc tgtaaacaag gataatgcca
     2821 atgctgtatc tagcccagtc actatcaagc atgaagatta tgatagcgtt gatacgaatg
     2881 gtgttggaat ggcaccatac atgatttcag ttgcgttgat ggtggtagct ttatcagcca
     2941 atgttatttt tgcgaaagct ttatctggca aagagccagc taatcgtttt agttgggcta
     3001 aaaataaatt attaatcaat ggattcattg caactctagc agcaactatc ttattttttg
     3061 cagttcaatt cataggtctt aaaccagatt accctggaaa aacctacttt attatcctat
     3121 tgacagcatg gactttgatg gcattagtaa ctgctttagt gggatgggat aataggtatg
     3181 gttccttctt gtcgttatta atattattat tccagcttgg ttcaagcgca ggaacttacc
     3241 caatagaatt gagtcctaag ttctttcaaa caattcaacc atttttaccg atgacttact
     3301 ctgtttcagg attaagggag accatctcgt tgacaggaga cgttaaccat caatggagaa
     3361 tgctagtaat ctttttagta tcatcgatga tacttgctct tcttatttat cgtaaacaag
     3421 aagattaata gaaagtatct agtgatagac taacagtatg atatgctatg tcaaagtatt
     3481 taggaggaga agatatgtct actttaacaa taattattgc aacattaact gctttggaac
     3541 atttttatat tatgtatttg gagacgttag ccacccagtc aaatatgact gggaagattt
     3601 ttagtatgtc taaagaagag ttgtcatatt tacccgttat taaacttttt aagaatcaag
     3661 gtgtatacaa cggcttgatt ggtctattcc tcctttatgg gttatatatt tcacagaatc
     3721 aagaaattgt agctattttt ttaatcaatg tgttgctagt tgctgtttat ggtgctttga
     3781 cagttgataa aaaaatctta ttaaaacagg gtggtttacc tatattagct cttttaacat
     3841 tcttatttta atactactta gccgttcgat ttagttgaac ggcttttagt aatcattttt
     3901 ttctcataat acaggtagtt taagtaattt gtctttaaaa atagtataat ataactatga
     3961 attcaaagag aggtgacttt ggttatgact gagaactggt tacatactaa agatggttca
     4021 gatatttatt atcgtgtcgt tggtcaaggt caaccgattg tttttttaca tggcaatagc
     4081 ttaagtagtc gctattttga taagcaaata gcatattttt ctaagtatta ccaagttatt
     4141 gttatggata gtagagggca tggcaaaagt catgcaaagc taaataccat tagtttcagg
     4201 caaatagcag ttgacttaaa ggatatctta gttcatttag agattgataa agttatattg
     4261 gtaggccata gcgatggtgc taatttagct ttagtttttc aaacgatgtt tccagatatg
     4321 gttagagggc ttttgcttaa ttcagggaac ctgactattc atggtcagcg atggtgggat
     4381 attcttttag taaggattgc ctataaattc cttcactatt tagggaaact ctttccgtat
     4441 atgaggcaaa aagctcaagt tatttcgctt atgttggagg atttgaagat tagtccagct
     4501 gatttacagc atgtgtcaac tcctgtaatg gttttggttg gaaataagga cataattaag
     4561 ttaaatcatt ctaagaaact tgcttcttat tttccaaggg gggagtttta ttctttagtt
     4621 ggctttgggc atcacattat taagcaagat tcccatgttt ttaatattat tgcaaaaaag
     4681 tttatcaacg atacgttgaa aggagaaatt gttgaaaaag ctaattgaaa aagtcaaatc
     4741 actgacttct gtgattaaaa ttgtattttt tatatctgtt ttagtgctta ttattgttga
     4801 aatgattcat ttgaaacgaa ctatttctgt tgagcaacta aagagtgttt ttgggcaatt
     4861 atctccaatg aatcttttct taattatcct tgtgggggtt atcgctgtct taccgacaac
     4921 cggatatgac tttgtactga atggactttt acgtacagat aaaagcaaaa ggtatatttt
     4981 acagactagt tggtgtatca acacttttaa taacttgtca ggattcggtg gcttaatcga
     5041 tattgggttg cgcatggctt tttatggtaa aaaaggtcaa gagaagagtg acctaagaga
     5101 agtgactcgt tttttaccct atcttatttc tggtctgtca tttattagtg tgattgcctt
     5161 aatcatgagc catatttttc atgccaaagc tagtgttgat tactattatt tggtattaat
     5221 tggtgctagt atgtattttc ctgttattta ttggatttct ggtcataaag gaagccatta
     5281 tttcggagat atgccatcta gtactcgtat aaaattaggt attgtttctt tttttgaatg
     5341 gggatgtgca gccgcagcgt ttataattat cggttattta atgggcattc atctaccagt
     5401 ttataaaatt ttaccactat tttgtattgg ttgtgccgtc gggattgtat cccttattcc
     5461 cggtggatta ggaagttttg aattagttct atttacaggg tttgctgccg agggactacc
     5521 taaagaaact gtggttgcat ggttattact ttatcgttta gcctactata ttattccatt
     5581 ctttgcaggt atctatttct ttatccatta tttaggtagt caaataaatc aacgttatga
     5641 aaatgtcccg aaagagttag tatcaactgt tctacaaacc atggtgagcc atttgatgcg
     5701 tattttaggt gcattcttaa tattttcaac agcatttttt gaaaatatta cttatattat
     5761 gtggttgcag aagctaggct tggacccatt acaagaacaa atgttatggc agtttccagg
     5821 tttattgctg ggggtttgtt ttattctctt agctagaact attgatcaaa aagtgaaaaa
     5881 tgcttttcca attgctatta tctggattac tttgacattg ttttatctta atttaggtca
     5941 tattagttgg cgactatctt tctggtttat tttactattg ttaggcttat tagtcattaa
     6001 gccaactctc tataaaaaac aatttattta tagctgggaa gagcgtatta aggatggaat
     6061 cattatcgtt agtttaatgg gagttctatt ttatattgca ggactactat tccctatcag
     6121 ggctcatatt acaggtggta gtattgaacg cctgcattat atcatagcat gggagccgat
     6181 agcattggct acgttgattc ttactctcgt ttatttatgt ttggttaaga ttttacaagg
     6241 aaaatcttgt cagattggtg atgtgttcaa tgtggatcgt tataaaaaac tacttcaagc
     6301 ttacggtggt tcttcggata gcggtttagc ctttttaaat gataaaaggc tctactggta
     6361 ccaaaaaaat ggagaagatt gcgttgcgtt ccaatttgta attgtcaata ataaatgtct
     6421 tattatgggg gaaccagccg gtgatgacac ttatattcgt gaagctattg aatcgtttat
     6481 tgatgatgct gataagctag actatgacct tgttttttac agtattggac agaagttgac
     6541 actactttta catgagtatg gttttgactt tatgaaagtt ggtgaggatg ctttagttaa
     6601 tttagaaacg tttactctta aagggaataa gtacaaacct ttcagaaatg ccctaaatag
     6661 agttgaaaag gatggtttct atttcgaagt tgtacaatcg ccacatagtc aagagctact
     6721 aaatagtttg gaagagattt ctaatacttg gttagaagga cgtcctgaaa aaggtttctc
     6781 actaggatat tttaataaag attatttcca acaagcccca atagctttgg taaaaaatgc
     6841 tgaacacgaa gttgttgctt ttgctaatat tatgccaaac tatgaaaaga gtattatctc
     6901 tattgattta atgcgtcacg ataaacagaa aattccgaat ggcgttatgg atttcctctt
     6961 tttatcatta ttctcttatt atcaagagaa gggataccac tattttgatt tggggatggc
     7021 acctttatca ggagttggtc gcgttgaaac aagttttgct aaagagagaa tggcgtatct
     7081 tgtctatcat ttcggtagtc atttctactc atttaatggt ttacacaagt ataagaagaa
     7141 gtttacacca ttgtggtcgg aacgttatat ttcttgttct cgttcgtcct ggttaatttg
     7201 tgctatttgt gccctattaa tggaagatag taaaattaag attgttaaat aagctttatt
     7261 tggcaattaa aaagagcatg tcatgcgaca tgctcttttt aaatcattta ataccattga
     7321 ttgcttgaat ctactttata atatgatgtg cttttaaata ttgtttagct actgtagctg
     7381 ctgatttatg ctttacagct acttggtagt tcatttcttg catttctttt tcagtgatat
     7441 gaccagcaag tttattgaga gcttttttta cttgagggta ttttttaatc gtatcttgcc
     7501 ttagtagcgg tgctccttga tatggcggaa atagatgctt atcgtctttg agaatttgaa
     7561 gttgatactg gattaattca ctatcggtag agtaagcgtc aataatattg acatctttac
     7621 tattgatagc ttggtatcgt aaagcaggtt ctaaggttga gatatcaagg tttaaaccat
     7681 aatgtttctg taaaccaata ctcccatcct ctctatcctt aaattctaaa gtgaaaccag
     7741 cttttagttt atctaatttt tttaggtcgc tgatattttt gagttgatta gcttcagcgt
     7801 aatctttttt aacagctaca gcgtaggtgt tttgataagc cataggtgac aaaagtgaca
     7861 acttatcttg ttttaaaata ccgtttttgg ctaagttgta aacctgttta gggttattac
     7921 ttactttagg aggattttta agtaaacttg atgtaatagt acctgtaaac tctgggtaaa
     7981 gatcaatatc accagatttt agggcttgat ataagaaagt tgtttttccg aaattaggtt
     8041 taagcttaac tttgatatct gtctgatcct caataagttc tttgtacata ttaattaaaa
     8101 tatctggttc agttcctaac ttccctgcaa cagttatcgt tgcagtattt gttccaggca
     8161 tccattttgg agcataggat agtcctaagc ctgctagtaa aagtatacca gaaacggcta
     8221 tggttcgtaa tctagccttt tctagtaatc caattaaacc actaaatata atagctaaga
     8281 cagcagaaga gattgcccct atgagaatta gagaaggatt gttgcgatca atccctaaca
     8341 aaataaatga tccaaggcca ccagctccga taagactagc taatgtagct gtaccaataa
     8401 tcataacaga ggcagttcta atgcctgaga tgataacagg catacttaaa gctaactcaa
     8461 actttttaag cctttcccaa cgtgtcattc caaaagcggt tgctgcctca atgagattag
     8521 catcaatttg catcagtgct gttacagtat tttgtaaaat tggaaataga gcgtaaataa
     8581 ttagtgcaac gacagctggt acagttccaa ttcccataaa aggtataaat aaaccaagca
     8641 aggctaaaga aggaattgtt tgaaatatcc cagttatctg aaggacaaca tgactgattt
     8701 ttttataatg actaatgatg ataccgagtg gaatagcaat taaagttgct aaaataagtg
     8761 ttagtagtga tagctggagg tgttcaataa gactttgagt ccaatctcca aaacgctctt
     8821 gaaaggtagt gattagtgtt gtcatactca gttctcctct tcaaataggg ttctaacaaa
     8881 ttggtcagag ggattatttt gaattatttc tggtctatcc aattgcacta tcttaccttc
     8941 cttaataaca caaattctat ctgcaagacg catagcttcc ttcatgtcat gggtgacaaa
     9001 cactaatgta atacccaatt cagactgtaa ttgtttggtg atatcttgta attgacgtct
     9061 tgagataggg tctaaagcag aaaatggctc atccataagt agtaccttag gtttagcaac
     9121 gattgctcgt aggataccta tgcgttgttg ttccccacca gagagctcat gaggatagcg
     9181 attaaagtag tccttagcag gtaaacccac cttatcaaga aggtcgctag cggctttttt
     9241 ttggtcacct tttgaccagc ccttcatttc agggataagt tctatatttt ctccaactgt
     9301 taaattaggg aaaagtgcaa tttgttgcaa gacataacca gtttctaagc gtaattgtcg
     9361 caaatcaagt gatgtaatgt tttccccatc taaagtaata gcgccttggc ttggttcgat
     9421 gagacgatta atcatcttga ggatggtagt tttaccactg ccactggccc ccacaaggac
     9481 aaagaattct cctctttgaa tatggcaact aatattatca atgatggtat gatcaccata
     9541 tgatttacta acattttcaa atgaaatcat gtaatttact ttctagtgcg taacgagtgt
     9601 aggtcgtatg atattagggt tatattagca taaagtagac attcaaacaa ctatctgaca
     9661 tttaaattgt cagaaacgat aaactagaag caaaaacaaa caccttgcgg tagacaaggt
     9721 gtttgttgga gatttatgaa aagaaaagtt ttaggatggt tatagtatat aaggccatgc
     9781 ttaaacaagt cttaaaacgt tttactctgt aaaataagtt catcgaaaaa acgtttaagt
     9841 gaaattttac caccatacat gctttttcct tgaataagtt gtgcctcatt atggatattc
     9901 tgaaatccaa acaatgcatt agcatattgc agtgttaatt cattttcaaa atcatcaatg
     9961 tacagatgag ccatatttat catagctttt ttaacggcac gacgaatacg ttgtaaaatg
    10021 attttttgtt ggtgttcatc gatagaaagt tctttttgaa aatcaaattg ggcaaaattt
    10081 aattcttgtt ttaataaaag ttcacagatg ttaagaatat ctgtgtaggc tgtctctgaa
    10141 gtaataccta aataactgag tattgaacgg attttagtga ggttactagt cgttataggc
    10201 ttttgatatg atggcgtatt ttctaggaga ttttggattg tatttaattt tttttgcatt
    10261 tcgatagtat cagtgacacg tttcacgacg ctttttactt caataatatt aatgggttta
    10321 ttaataaaaa attcgattcc tgctttatat gcttcttgtc gtaaatcatt atctttgact
    10381 tgggaaatca taataaattt taaatcggaa cgctgtttat agattttttg gactaatgtt
    10441 acaccgtcta atattggcat taataaatca atcaatacga tatcaacatc tgcaatcagc
    10501 aattcgttgt atgctttact ggagacatta ttcactctga ctactgtatt attgaaatct
    10561 tcttcaataa tatcttgtaa aatcatagtg atagtggggt catcatcaat aatatagaat
    10621 ttcacgtatt atctcctaac ttaaatcgat ttttacggaa taacagtgta aatgttgttc
    10681 catttggctg attaggacaa acagttatcg ttccttgata ttgctcttcc attaaaatac
    10741 gcacgtggga tagcccgatt cctcgataga tatcaccatt agcatcaaat ttagtcgaaa
    10801 atccaggttt gaaaatcatg tgtttcattt tatcaggtat tcctggtccg ttatcactaa
    10861 tctcaatttt aataaaatct tctaattctt ctgtgtgaac actgattgta cccttttttt
    10921 gtttatcaat agcttctact gcatttaaaa cgatattgga aataatggtt agtaaatagt
    10981 aatagtttga tattatcaaa ttacatttat tattttcgag aataatgata tcttgatgcc
    11041 gaaattgaat aatactccta gtataagata aaacaatttg aaagatatct ttcaatgcca
    11101 tagttgattc attttttaca ctaaaatagg ttcctaagcc tttaataata ttttggtaat
    11161 ctttcttaac ttcgtgaacg tcccgcgaaa tatctagaga gaggtgttgg tattctttag
    11221 gtaggtgata tttggataac tctttatcta ataaataagc atttttcatg atgttttcaa
    11281 tttcaatgat atttttttgc ataaaataaa cctcgctttt aacggctgaa gttgaccaca
    11341 taaaataata gtatcgacgt tcgtgatcgt ctttttgtaa gagaagtgat agataactat
    11401 aagtataagc cacagcgcaa ctaataaaag ctcgcaaaag tgctatggca aataaagtag
    11461 cgaatatagt tatagtataa ttgttaaact ttatgagaaa actaatttct acaaggttgg
    11521 caaaataatc acaaataatg atagaaaaga aaaaagtacc tttattacgg taggaacgat
    11581 gccaataaat agtataaaag gtgatgccat aacaaatata aaacgccata tcagttaaaa
    11641 cgaattcaat tatttgatga atctcagcct cgccagcgat acttaatatg attcctctga
    11701 agataggaga agcaaaagta attcctagac agagaagaat tggattaatg tcgtcattaa
    11761 aatataaaaa gacaggcaaa atgaacaaag ataaggtcat aataaaccca tcagcaagta
    11821 ttgagatatt gatttgagca gctaaggcga tagctattgc agagataata atacgctgcc
    11881 ttctttctag atgtttttct gataatatat ctagcatata aatgtcctta aagttttttt
    11941 gaagattttt gatgaatttt gattattttt gaaagcgcta tctacaataa gagtataaaa
    12001 aaaaaggagg caataagcaa tgaaaaattt acgtaaccgt agttttctta ctttattgga
    12061 ttttagtaca gcagaagttg aatttctttt aaaactttca gaagatttaa aacgtgctaa
    12121 atacgcagga attgaacaac aaaaacttgt tggtaaaaat atcgctttga tttttgaaaa
    12181 agattctaca cggacacgtt gcgcttttga agttgctgct cacgatcaag gagctcatgt
    12241 tacttattta ggtccaacag gcagtcagat gggtaaaaaa gagacctcaa aagacactgc
    12301 gcgtgtgcta ggtggtatgt atgatggtat tgagtatcgt ggattttctc aagaaacagt
    12361 tgagacttta gccgaatttt caggagttcc tgtgtggaat ggtttaaccg atgctgatca
    12421 tccaacgcaa gtactagccg attttctgac cgctaaggaa tgcttacata agccctataa
    12481 agatattcgc tttacgtatg tgggtgatgg acgtaacaat gtcgctaatg ccttgatgat
    12541 cggtgcttct attgtaggca tgacttatca tttggtttgt cctaaagaat tagagccaga
    12601 tcctgaacta ctctctaaat gtcaagaaat tgcgaaaact actggtgctt ctattgaaat
    12661 cactgctgat attgcagaag gcgtcagaga ttcagacgtc ctatatacag atgtttgggt
    12721 ttcaatggga gaaccggatg aagtttggaa agaaagaata gctcttttag aaccctatcg
    12781 tattacccaa gaaatgctta atatgacaga aaatccaaac gttattttcg agcattgctt
    12841 gccctcattt cataatatag atactaaagt tggatacgac atttatgaaa aatatggttt
    12901 gaaggaaatg gaagtatcag atgaagtttt tgaaggccct cactcagttg ttttccaaga
    12961 agctgaaaat cgtatgcata ctattaaagc agttatggta gcaactttag gtgattagga
    13021 ggaattttca tggcaaaaat tgttgttgca ctaggaggaa atgccttagg aaattctcca
    13081 gaagaacaat taagattagt aaaacatact gcaaaatcat tggtggcctt gataaaaaag
    13141 ggacacgaaa ttgttgtaag tcatggtaat ggaccacaag ttggtgccat taatttagga
    13201 atgaattttg cggctgagag tggtcaagga actaattttc cttttccaga atgtggagca
    13261 atgagccaag gatacatagg ctatcatctc caacagagcc ttttaaatga attacgtcaa
    13321 gagggaataa ataaggaagt tgctacaatt attacccaaa ttgaggtgga tgaatcagac
    13381 caagcattta gcgcacctac caagcctata ggcacctttt atgataagga gacatcagaa
    13441 aagattgcta tagagaaagg gtatactttc gtagaagacg ctggacgagg gtatcgtcgt
    13501 gttgttgctt cacctgaacc taaaaaaatc atcgaaatta atagcattaa aacacttatt
    13561 gaaaatgata ccctagttat cgcaggtgga ggtggaggca tccctgtcat taataaggga
    13621 ggttatgaag gtattgcagc tgttattgat aaagacaaat ctagtgcact gttagcagga
    13681 gaactagctg cggatcaatt gattattctc actgcagttg attatgttta tactcaattt
    13741 ggtaaagaaa atcaaaaagc tttaacagag gttaatgaga atcaaatgat tgattacgtt
    13801 aaccaaggtg agtttgcaaa aggaagtatg ttacctaaag taatagcctg tatgagtttc
    13861 ttagatcaca atccgaaagg aatagcctta atcacctcat taaatggttt agaagatgca
    13921 ttagatggga aattaggaac acgtataact aagtaatctc agataagtgc aaagaaatga
    13981 ttatttaacc ttcatctttg cactttttct gatgaagtca atctattttt attatagagg
    14041 aggcgtgtca tgccagaaaa aatgacaaac agtgaaaaca aaaggagtaa aggttttcga
    14101 atgccaggtg cctttacaat attattcatt ttaacaatct tttcggtttt ggctacatgg
    14161 tggatacctg ctgggtccta ttcaaaatta caatttgata cagcatcatc caaactagta
    14221 gtaacagatc caaatggtaa gacagttcat gttccagcaa cgcaaacaca gttggataag
    14281 atgaatgtca aaattaaaat taaagaattt acttctggtg ctattagtaa gccagtttca
    14341 gttccaaata cttacaaacg tttgaagcaa aatccagcag gcattggctc cgtgacgact
    14401 agtatggtaa atggaaccat tgaagctgtt gatattatgg tatttatcat ggtacttgga
    14461 ggcatgatag gagtagttag aaagagtggc gcttttgagt ctggtcttct agctttaaca
    14521 aagaaaacca aaggtagaga gtttttattg atatttttag tttccttatt gatggtttta
    14581 ggcggtactc tatgtggtat tgaagaagaa gcagttgctt tttatcctat tttagttcca
    14641 atctttttag ccatgggtta tgattctatc atttgtgtag gtgctatttt cttagcaagt
    14701 tctgttggga catcattttc aacaattaat ccgttttctt ctgttattgc ttcaaatgca
    14761 gctggtatta gtttcacaga aggcttatca tggcgtacag cagggtgtat tgcaggtgct
    14821 atttttgttg tggtgtatct acattggtat gctaaaaaaa taaaagcgaa tccagagttt
    14881 tcatattcct atgaagatag agtagaattt aatgctaaat ggggcatgac aactaaccat
    14941 acaccctcac tctttactat tagacaaaaa attattttaa gtttatttgt tatttcattt
    15001 cctttgatgg tttggggagt aatgtcacag ggatggtggt ttccaaccat ggcttcttcc
    15061 tttttagcta ttacaattat tattatgttt ttaacagcaa ctggggcaaa tggtattgga
    15121 gaaagggatg ttgttgatga gtttgttaat ggtgcatcaa gccttgttgg ggtttcatta
    15181 atcattggtt tagcgcgtgg tatcaatatt attttgagcc aaggttatat atcagatacg
    15241 atgctttaca cagcttcaaa attagcttca catgttagtg gccctgtctt tatcattgtt
    15301 atgatgttta tctactttgt gttaggattt gttgttccat cctcgtcggg tttagcagta
    15361 ctttcaatgc caattctagc acctcttgca gatactgttg ggattcctcg ttcagtagta
    15421 gtaatggctt atcaatttgg gcagtatgca atgttgtttt tagcacctac tggactagtt
    15481 atggcaacct tgcagatgtt agatatgaag tactcccatt ggctaaaatt tgtgtggccg
    15541 gttgttttat tcctcctaat atttggtggc ggtttgttag ttctacaagt tttaatactt
    15601 taaagatttc agctattagt ctcaaaaata tctgaagaga tataataaat tgagtttaag
    15661 ttaaataaaa aaaacgatag gtgtaatcta tcgttttttt tatttccttt ctctagtctt
    15721 tcttcaattt tggaaaggtg agcgtaaact gactgccatc atcaggttta gaatcaaccc
    15781 atattttagc acggtgtatt gtagcgattt gttttactaa agatagccct aaaccaattc
    15841 cagactgact atccttggtt cgagcagagt ccacttgata gaaacggttc caaattttac
    15901 tttggtcatc aactgagata cctagaccat tatctttaat agagaagaca atttgattat
    15961 cggactgtct tagactaata gaaatatgat tgtttgtaaa tttaatagca tttgaaaata
    16021 gattatcaaa aagccgtttg agtagtgttt gttgacctat aatccaaagg tcatcttcga
    16081 tttgagagtc aaggtgtata ggggtattat ctaataatct actcgaatcg gatactagtt
    16141 gcttcatcat aattgagaaa ttaagaggtt ctaactgaat tgataatgga ttttctgcct
    16201 tagtcaattc tagaagttgc tcaactaatt ttttcattga tagtgactga cggtgtataa
    16261 cctcaaagga ttcttttgct tcacttaaat tttctgcgta gttttttccg tattcacttt
    16321 cagaaagtat aactgttaca ggagtccgta gttcatgaga aacatcattg ttaaattgtt
    16381 tttcccgaac aaatgatgat tcaatagaag ccaacatagt attgataaca gctgctagct
    16441 cagttaattc attttcgtta tttttagtga taattcgctt agtatagtct tcattttggg
    16501 taataaattg agcggtttca gtaatttctt gaatcggtct aaaagcgcgc tttaaaatta
    16561 aatagccacc aacagttatt ataatcaaca taagaggaag aacgataccc aaactaaata
    16621 acaattcagt taattgctta tccagtctgt caacagtacg gatagcacgt aaccactgat
    16681 ttttcccaga aattggaata tcaacatact gaaaggtacg attctccatg gaaaatagag
    16741 ataatttctt tttatgttta tgttgatggt ctaaatccct tttaaatcct tttggaaaac
    16801 cagaatagat aacttttcca ttttggtcat agactgaata aaatattcca tcttcaaaag
    16861 cctcataccg atgaccttta gctaacgctt gactcatttg cacagcttta gcttctaaat
    16921 tttttttaga tttgttttca gcaatgcttt ttgaaataaa aaatgaacca attaatacac
    16981 cgataacaag gataaaaata aaagagctgt accagagggt aatttgagtg atgatgctag
    17041 actttttagg gtaatttatt ttactttgga ataacatatc ctaaacctcg tttagttttt
    17101 attagtgatc catctcgatt gttatctagt tttcgacgaa ggtttttgat aaggacatca
    17161 ataatattgg actctccata ataatcataa tcccaaacgt gttcacgaat ttgatgacga
    17221 gagaggactt ggtctctgtg acgtgccaaa tactcaagga cttcgtattc cttagctgtt
    17281 aaatcaatca aattgtcgtt tctccaaact tgctttgtag ataaattgat tgttatatct
    17341 tgaatttgaa tatcatcaga agatacttgt ctatttgtac gtcgtaacat agctcgaatt
    17401 cgggccatga gttctccaaa ttcaaatggt tttacaaggt aatcatccgc accaaaatct
    17461 agacctgcga tacgatcttc aacggcatct cgagcagtca acataagaat agatacttga
    17521 cttcctttgt tgcgaagcag ttttaaaaag gtgaagccgt ccatttttgg catcattaca
    17581 tctaagataa tcacatcata ttctgccact gaaacaaatt gtaaggcttc ttcaccattt
    17641 gaagcactgt caacactgta gtgttgcttt tttaaaagtt ttacaatact acggttcaag
    17701 tcaaactcat cttcaacaac aaggattttc attttttggc ctccgtatgg cttttttcca
    17761 ttatatcaca aaagggaatg gagtactgag gtcctattcc ctttaataca ctagtattag
    17821 tcgtgatttt tatgagttgt attaagctta tttgccttta taaatgatgc tttatcacta
    17881 tcaaattggg caatcttagg agtatttcca tcaagtagtt ttactttata aactagctta
    17941 tttgatttgt tattcgcaag agaaatttct tttatttttg catttttata ttttgcttga
    18001 atagcttgta atgcagtctg aatatcttta cttggttgtt ttaattgggc atcttttgaa
    18061 ttgagttgat tatctgatgt tgttgaaagg atcttcccag aattagcatc aattttatat
    18121 tttttagcta ttttattatc aataactttg atttcataaa aaggtgtatc acctttggta
    18181 tttaattcaa tttgtgaaat aaagcctgag cgagcttctt ttttggcagt ttctaaggca
    18241 gtcttataat cgatttttgt cgagatattc tttaatgttt ttagtgagta tttttctcta
    18301 tgatcgtgat gtttatcgtg tccttcatgc ttatgttcaa aagacatgtg ttctgagtga
    18361 tggttatgcg ctaatgcagc tccacttact cctatagctc ccccaatacc aagtagcgta
    18421 atggaagcta accctacgta taatttattg ctcatgaatt ttttaaagtt gtttttatag
    18481 tttgtcatac tgacttcctt ctttcttttt tcaaaccttg gattaggtt
//
LOCUS       DATRSG010000042        18422 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRSG010000042 DATRSG010000000
VERSION     DATRSG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18422
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.042"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            470..1291
                     /locus_tag="VBD89_001361"
     CDS             470..1291
                     /locus_tag="VBD89_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7135884.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            1392..2006
                     /locus_tag="VBD89_001362"
     CDS             1392..2006
                     /locus_tag="VBD89_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO7135885.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            2166..3605
                     /locus_tag="VBD89_001363"
     CDS             2166..3605
                     /locus_tag="VBD89_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO7135886.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSHKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            3696..4829
                     /locus_tag="VBD89_001364"
     CDS             3696..4829
                     /locus_tag="VBD89_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7135887.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            4925..5605
                     /gene="radC"
                     /locus_tag="VBD89_001365"
     CDS             4925..5605
                     /gene="radC"
                     /locus_tag="VBD89_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO7135888.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(5618..6256)
                     /locus_tag="VBD89_001366"
     CDS             complement(5618..6256)
                     /locus_tag="VBD89_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO7135889.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(6411..6758)
                     /locus_tag="VBD89_001367"
     CDS             complement(6411..6758)
                     /locus_tag="VBD89_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO7135890.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
     gene            complement(6760..7875)
                     /locus_tag="VBD89_001368"
     CDS             complement(6760..7875)
                     /locus_tag="VBD89_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7135891.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            complement(7879..8853)
                     /locus_tag="VBD89_001369"
     CDS             complement(7879..8853)
                     /locus_tag="VBD89_001369"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7135892.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            complement(9030..9602)
                     /locus_tag="VBD89_001370"
     CDS             complement(9030..9602)
                     /locus_tag="VBD89_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO7135893.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            9712..10383
                     /locus_tag="VBD89_001371"
     CDS             9712..10383
                     /locus_tag="VBD89_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7135894.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            10358..11194
                     /locus_tag="VBD89_001372"
     CDS             10358..11194
                     /locus_tag="VBD89_001372"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO7135895.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            11191..12075
                     /locus_tag="VBD89_001373"
     CDS             11191..12075
                     /locus_tag="VBD89_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7135896.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            12101..13093
                     /gene="pta"
                     /locus_tag="VBD89_001374"
     CDS             12101..13093
                     /gene="pta"
                     /locus_tag="VBD89_001374"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO7135897.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            13155..13325
                     /locus_tag="VBD89_001375"
     CDS             13155..13325
                     /locus_tag="VBD89_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO7135898.1"
                     /translation="MISRKVALVTGASAGFGAAVVTKLVSDGYSVIGCARRMDKLKCL
                     GEKFSEGYFYPL"
     gene            13326..13916
                     /locus_tag="VBD89_001376"
     CDS             13326..13916
                     /locus_tag="VBD89_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO7135899.1"
                     /translation="MDITSRESVDKALESLPKNLQSIDLLVNNAGLALGLDKSYEADF
                     EDWMTMINTNVVGLIYLTRCILPKMVEVNRGLIINLGSTAGTIPYPGANVYGASKAFV
                     KQFALNLRADLAGTKIRVTNLEPGLCEGTEFSTVRFKGDHKRVEKLYEGAHAIQAEDI
                     ANTVSWVASQPEHININRIEIMPVSQTYGPQPVYRD"
     gene            14051..16051
                     /locus_tag="VBD89_001377"
     CDS             14051..16051
                     /locus_tag="VBD89_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO7135900.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(16122..16793)
                     /locus_tag="VBD89_001378"
     CDS             complement(16122..16793)
                     /locus_tag="VBD89_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135901.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            17085..>18422
                     /locus_tag="VBD89_001379"
     CDS             17085..>18422
                     /locus_tag="VBD89_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7135902.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
BASE COUNT         5848 a         2838 c         3553 g         6183 t
ORIGIN      
        1 tttgcttaga gtgtttctaa taacttcaaa atccttacag ggtcgtttaa tctccttatc
       61 tcatgtaagg attttttgtt gtattaatat gaccaaattt attaatatac tcaattattt
      121 attttttcaa aacagaataa ctatgtgaaa aaaaaataat gttaggtctc tcaaaaacac
      181 gtatagcaat ttcgaatctc aaatatttta ttatgactat tgtaatagtt acgaatctat
      241 tattcaactc aacaataaga aaactcttca gtaaatcatt atgttaaaca agctctgata
      301 gtatggagat aagtataggt aaaagtcgat ttaatctctg aaacaaaatt taacttaatt
      361 aatagaaccg ttgacgtatc aggtatcaaa tctttttggt ttatatgtga tataatgaaa
      421 gaacatattg agatatttgg caccctcttg aaaggcaaag gttacttgta tgattaaact
      481 aatagcaaca gatatggatg gaacttttct acgctcagat aaaacttatg ataaagcacg
      541 gttttcttct ttattaacgt tgatggagaa gtatgatatt aaatttgtag ctgctagtgg
      601 taatttatac gatcaattgt tgttaaactt tttggaatac cctaatcgta tcgcctacgt
      661 cgctgaaaat ggtggtcgtg tcattgacca agatggcaca ttactaaaag aaacttattt
      721 atctaatgat acagttgcag ctgttttatc ctatttatat cagaactatc ctgagacact
      781 catcagctta tctggtgaaa aacgttcata ccttgagcga agaactccta ttaataggcg
      841 gactgaatta gaatactata tgcctaattt tatctataaa gaccatttgc tgcccttaga
      901 tgatgaccga tattttcaaa tgacactttg ggtaaatgaa aatcttgttt ctgagatgtt
      961 gttagatatt agtgaacact ttaaaaatca tcatatcaga ttgacctcta gtggttttgg
     1021 ttgtatcgat gtcttaccag ctgatgtcaa taaagcggat ggtatcgcta ttctccttga
     1081 aaagtgggga ttgaaacagg atcaagtgat ggtttttggt gatgggggca atgatgtgga
     1141 aatgttgaga gcagcaaata tttcttacgc tatgtcaaat gcgccagaag aaattaaagc
     1201 tattgctaaa tatcaaacag ttagtaacga tcaagatggt gtgttagaaa cgatagagaa
     1261 ctttcttcac aagatgagtg ataatgaata gaaccacgta gcttttcacg aagcatacta
     1321 ttcaattctc aggtgaaatt ttgttataat acaagaaagc ctagagaaat atttaagaat
     1381 gaaggagaac gatgttagaa gttattgaca aagctttaag agattatcaa atgaaacgag
     1441 aacagttttt tgagattaac aatcagacag tacaagaagg tgccattgtt ttcacaggag
     1501 attctatcgt tgagtttttt cctttaaaaa aacatttagg acgtgactat cctttagtta
     1561 atagaggagt agcaggatca gattcttatt ggcttttaga aaacttgaga acccaagtat
     1621 gggaactatt accatccaaa gtatttattt taattggaac aaatgatatt ggtttagggc
     1681 attcacagtc agaaatcata gctaatatca cggatataat tgcagaaatt cgtgcggaat
     1741 cttatatgac agagattaat atactgagtg ttttacctgt tagtgaagaa gatgactata
     1801 tagagcgtgt caaggtaaga aataatcaag caataaaagc tttaaacaaa accttatcag
     1861 taatttctgg cattaattat attgaattgt atgatttatt agttgacgaa aaaggtcagc
     1921 ttgctagcag ttttacaaaa gatggtttgc acctaacaga tcaagcctat gctaaaattt
     1981 ctgaaacgat aaaactccac ctttaaaagt gtttagatac tatttttaag tatttaaata
     2041 cacagaatta gtgaaaagac cttctaagta gaaagttcat gtcacggata gtaccagtat
     2101 ttggcgaatc aatgtcagta actggtcttt tttaatatta tgataagaaa aaacaagagg
     2161 ggtaaatgat ggtaaaacaa gtatttccaa aaggtttttt atggggcggg gcaactgctg
     2221 ccaatcaatg tgaaggagct tacaatgttg atggacgtgg cctagcaaat gtagatgttg
     2281 ttcctactgg agaagatcga tttgcaatta tttcaggaca aaagaaaatg tttgattttg
     2341 aggaaggata cttttatcca gcaaaagaat caatagattt ttatcaccat tataaagaag
     2401 accttgcatt acttgcagaa atgggtttca aaacctatcg tatgtcaata gcatggacgc
     2461 gtatttttcc aaagggtgat gagctatatc caaatgaagc tggtcttcgg ttttatgaaa
     2521 atatttttaa agagtgtcgt aagtatggta ttgaaccttt ggtaaccatc acacattttg
     2581 actgtcctat ctaccttatt aaacattacg gtgggtggcg gagccataaa atgattggtt
     2641 tttatgagcg ccttgtacga gctttattta ctcgttttaa ggggttagtt aaatattggt
     2701 tgacctttaa cgaaatcaat atgattttac acgctccttt tatgggcgct ggcttatatt
     2761 ttgaagatgg tgaaaatcaa gagcaaatta aatatcaagc agctcaccat gagttagtgg
     2821 cttcggctat tgcagtaaaa attgctcatg aagttgatcc aaatagtcaa atcggatgta
     2881 tgttagctgc aggtcaatac tatccaaata cgtgtcatcc acaagattat tgggcttcga
     2941 tgcaaaaaaa tagagaaaat tattttttta ttgatgtgca agcgcgtggc aaatacccta
     3001 attatgctaa aaaatatttt gagcatttag gtatttcaat tcaaatgaca gcagaagatc
     3061 ttgctttgtt aagagactat acggttgatt ttatttcatt ttcttactat tcaagccgag
     3121 tagcttcagg taatccaact gtcagtgaac aggttcaaga aaatattttc gcatctctta
     3181 agaatcctta cttgaaatct tctgaatggg gatggcaaat tgatccgctt ggattacgca
     3241 ttaccttaaa tgctatctgg gatcgttatc aaaaaccgat gtttattgtt gaaaatggac
     3301 ttggagcagt agatattcct gatgagaatg gttacgtaga agatgattat cgtattgatt
     3361 acctccgtca gcacattgct gcaatgagag atgctatata tgtagatggt gttaatctaa
     3421 ttggctatac tacatgggga tgtattgatt tagtatctgc aggaactggt gaaatggaaa
     3481 aacgctatgg tttcatttat gttgatcgca ataataaagg agagggaaca ctcaagcgtt
     3541 acaagaaaaa atcgttttat tggtacaaga aggttattgc aagtaatggc agtcaaatag
     3601 aataaagagc agactaataa ctgttaactc tctttttctc attcataaaa tagtgtataa
     3661 taattgaaag aaattaagat taataaggag atttcatgaa atttgaaaaa cggcaggtct
     3721 attatgttgt cataacattt gctatttgct atgctataca ggcttattgg ggagctgttt
     3781 ctaatatttt aactacgctt cataaggcaa tatttccttt tttgatggga gctggaattg
     3841 cctatattat taatattgta atgtcagtct atgagcgatt atatataaag ctttttaaag
     3901 gatctagact attaatggca atcaagcgta gtgtttctat gattttatcc tatgcaactt
     3961 ttattggttt aattgtctgg ctattttcaa ttgtcattcc agatttgatt tctagtttga
     4021 gttctttatt ggttattgat accggagcac ttgctaaatt ggttaataat ctcaatgaaa
     4081 ataaacaaat ttctgaggct ttaaattata tgggaacaga taaagactta gtttctactt
     4141 taagtggtta tagccagcag attttgaagc aagttttatc tgttttaaca aatttactaa
     4201 cctcagtttc ctctattgcg gcaacacttc tgaatgtttt tgttagtttt attttttcaa
     4261 tttacgtttt ggcaaacaag gagcagttgg gacgtcaatt taatttgtta attgatacct
     4321 atttaggttc aacaggcaaa acattccatt acgttcgtca tatccttcat caacgtttcc
     4381 atggtttttt tgtaagccaa actttagaag ctatgatttt aggaagtttg acggttattg
     4441 gtatgttgat cttccaattt ccttatgctt taacagttgg ggttttagtt gcttttacag
     4501 ctctaatacc ggttgtggga gcctacattg gtgttacaat cggtttcatc ttaattgcta
     4561 ctgaatcgct tactgaagca ttcttgtttg ttcttttctt gatcctttta caacaatttg
     4621 agggaaatgt catttatccg aaagttgtcg gtggatcgat tggactgcct tctatgtggg
     4681 ttttaatggc tattactatc ggaggtgctt tatgggggat cttaggcatg ttacttgctg
     4741 ttcctgttgc agctactatc tatcagattg taaaagatca tattatcaag cgacaaacgc
     4801 ttagaaatcg tgcacgaacc tatcgttaaa taaaaaatac ttagattttt catctaagta
     4861 ttttttattg atttaaaaca tgagtttttg attagtttac gaatattatg actggaggtg
     4921 ttttatgtac catattgaat taaaaaagga agctttacta ccaagagaac gcctagttga
     4981 tttaggcgca gatagattga gtaatcagga gttattagcc attctcttac gtacaggtat
     5041 taaagaaaaa cctgttcttg aaatttcaac gcaaatttta gaaaacataa gcagtttagc
     5101 agattttggt caattatcct tacaggagtt gcaatccatt aaaggaatcg gtcaggttaa
     5161 atccgtcgaa ataaaagcta tgctagaact agcaaaacgg attcacaaag ctgaatatga
     5221 tcgtaaagag caaattttaa gtagtgaaca attagcgagg aaaatgatgc tcgaattagg
     5281 ggataaaaaa caagaacatt tagtagctat ttatatggat acacaaaatc gtattatcga
     5341 acagagaact atttttattg gtactgtacg tcgttcagta gcagagccaa gagaaattct
     5401 acattatgct tgtaaaaaca tggcaacttc tttgattatt atacataatc atccctcagg
     5461 ttctccaaat cccagtgaaa gtgatttaag tttcactaaa aaaataaaac gatcatgtga
     5521 tcatctggga attgtctgcc tagatcacat catcgttgga aaaaataaat attatagttt
     5581 tcgagaagaa gcagatattt tataaataat gaactattta tcgctgctgg ttcatgaaat
     5641 aaagtaaagt ttgtaattcg cttgttaaat ctacatactg aacaatgata tcttttggta
     5701 atgttaaatg aactggagaa aaactcaaga tgccttttat acctgctttg actaagatgt
     5761 cagcaacttc ttgggcttct gtactaggta ctgttaggat agcagtttca atatcactat
     5821 ctattaaatg gtcattgata gtcgaaatac cgtagacagg aattccatcc tcggttgttt
     5881 taccaactaa atcattgcta tctaaatcaa aagccattga aatttgcatt ttattgcgat
     5941 cgtggaaacg ataatgcaag agagctctac cgatatttcc acaccccacc agcataacat
     6001 ttgttgtaga atgatcgttc aatatttctg caaagaagtt cataagtttt ttgacatcat
     6061 aaccaaaacc acggcgtcct agttcaccaa aataagaaaa atcacgtcga acagtagcag
     6121 aatcgatacc tagggcatct gcaatttgtt tggaactagc tttttcgatg ccatcagtat
     6181 taaaacgttt aaaaatacgg tagtacagtg ataaacgttt agcagttgct ttaggaatag
     6241 acttatccat aatcacaaaa ttatctcctt gttaaaaatt ttattattcg atttagaatc
     6301 agttatataa cttaattctt cacatctcta ttgtacaaaa agattgtgaa agtgacaaca
     6361 aaatggttcg caaaaagacc actaaaatgt taaaaatagt ggtcggtaaa ttattttgca
     6421 gttttcgtaa agatattacg ctcaacgagg ctgtccatta agaaataaaa tttgtcttga
     6481 agaattttat gatcggtaga tttaaagata tttactattc taaggccaga tttatcagta
     6541 attaagagct taaaaccaga aagttcttta ttgattgtaa tctttagagg aaagccatcg
     6601 ccagaattag gtactgtttc taggagacgc tctaattcat aattacctaa gcgattttga
     6661 gtgaatgtag tatcgcgtaa ggtatatttt ttaatgttcg ggctgatagt gtaaatgtaa
     6721 ttgcagtctg gaagatttat ttctttttca aatgccatat taatctccta taatttttct
     6781 taaggtttta gctaattcaa gaatttcttc ttgggtattt agttctgaaa aactaatacg
     6841 tattgattca tttagacgtg gtgagttggc tccatacaag cttgttaaga catgactagg
     6901 ttcgactgtt cctgcagtac atgcagaacc tgttgaaact gcgaatccag ctaaatctaa
     6961 ctgtgtcaat aacaagccat tattctgtct aggaaaacct atattaagta catgaggtaa
     7021 gcagtcttga ccgttattaa gatagaagtc aaggtctgaa atagcatcta aaaaggtcgt
     7081 tctcagctga ctaatgtgag tccaattttt aagggtgtta gtcgtagcat cagtaagagc
     7141 ttgagacatt ccagcaatac caattatatt ttcagttgaa gcacgccttt tttcctcttg
     7201 gtctccacca tgaagtagac tatcaaagtg atggggagca cagtaaagta tcccaacacc
     7261 ttttggaccg tgaaatttat gggcagaagc agctaaaaag tcaattccta aagaatgagg
     7321 atcaagttcc attttgctaa agacttgaac ggcatcaacg tgaaaaacag cttggtggtt
     7381 cctgagaagc tgaccaatct cttgaattgg taaaaagtcc ccggtttcat tattagcaaa
     7441 catgagagat accataatag tatcatctcg taaagcttct tgaacgtctt ttgcagtaat
     7501 ttgtccatgg tttggtttta agtaagtaat atcaaaacca aatcgctctg ataagtaagt
     7561 catagtatgt aggactgaat gatgttcaat ttcagaggta ataatatgtt taccttttag
     7621 ctgatttgca agagcatacc ctttgatagc tgtattgttg ctctcagttc ccccagaagt
     7681 gaaaataatt tcacgtgaat taacatttag atattcagca ataatttgtc gacattcacg
     7741 taaaagttga tttgcttgac gcccaaaggt atgtatacta gatggattac catagttact
     7801 tgtcatgaca ttggtcattt tctcaataac agatggggtt agagcggtag tagcagcatt
     7861 gtctaaataa atcatcaatt aatccttctt atctgaacga tatgaaaata atggacttaa
     7921 aggttttctt tcatggatac ggataatagc gtctgcaatg agatggctag cggtcagata
     7981 tttaatattg ctaggaatac gctctttaga taaaacagag tctgttacga taatttctct
     8041 aattggtgca gactctaaaa tatcagctgc gccgccagca aaaagcccat gactagcaac
     8101 agcataaatc tcagttgctc caccacgttc taaaattttt gcagcttcgg caaaggtttt
     8161 tcctgtattt aaaatatcat caataataat agctttctta ccttctacct caccaataat
     8221 atagccttct tcacgttcag aatcatcttg tgcataatca atgatagcaa ttggagagtc
     8281 aaggtattct gccaaactac gagcacgctt gattccagaa tttttaggac taacaacgac
     8341 gacatcttca ccagagagac cgagttgatt atagtgttca gcaaatagtg ggacagtaaa
     8401 aagattgtca actgggatat cgaagaaccc ttgtacttgc actgcatgta agtcgagggt
     8461 aagaactcga tcaactccag ctttaactaa catattagcg accaattttg cagtaatagg
     8521 ttcacgagat gcagcgatgc ggtcctgacg agagtatcca aaataaggga ctacgatgtt
     8581 aacagtatta gcacttgcac gtttgcaagc gtcaatcata atgagtaatt cccagagatt
     8641 atcattgact gggaagcttg tagattggat gatatagata tcgtcaccgc gcaccgtttc
     8701 ctcaatatta atcataattt cgccatcaga gaattgacgt gatgacatct tacccaaagg
     8761 aattcctgaa gcttgggaaa ttttttcagc aatttctcta ttagcggtta atgagaaaag
     8821 tttgatttgt ttatcggcgt attgttctgc catgagatag atgactcctt tagattgata
     8881 ctaatagttt accaaaaaaa tagaaaaaat tcacgataag aaacatgaaa atgcttgcaa
     8941 taacaatgtt gaggagagat agtatgagat tttaagttct tgcttaaatg agaattataa
     9001 aagagattgg aaaatccaat ctctcaacgt tagttatttt ttaaagtagc agagaatcta
     9061 gccaccttac ttttggcgta tttaaaatta atattatttt cttttaaaaa ctggtcgaaa
     9121 tctcttttcc cttgtttagg ttgtttgact tctaactcaa gttcataatc tttaatatca
     9181 gcatatatat tgctgtctag agccattaag ccgattgaag tttccatctc tcgtcttgtt
     9241 gttgtaagtt gtccaaagac agctaatttg gttggatcaa ctcccttttc aaggattaaa
     9301 gaagcaatct cagtatcttc agggaactgg ccatttttaa cgatatattt agcttcttct
     9361 aaagtaaggt catgattgtg ctctaaattt cctacttctc tagggatttt tagtgttagt
     9421 tcggcacggt taggtaacgt tctaatgcga agtgacattc ggtgtgcttt catttcaaat
     9481 gtttctgtgt caaaatagta attggtttga gtaataggtt gaacatgaga aaataagctt
     9541 gttagacgat taaattcgtc tttattcaat agagttttgt attcaatttc taggtgtgtc
     9601 atcttatcac ttccttttcc taaatatggt ataatattaa ttgttgtgtt aattttatag
     9661 aaaaaaagtt tattttacaa gagagtacat taaatatttt ggaggattag aatgtcaatg
     9721 gattgggaaa catttctaga cccttatatt caaacggttg gtgaattaaa aataaagctt
     9781 cgtggtattc gtaaacaatt tagaaagcaa aatcgtcact ctccaataga atttgttact
     9841 ggacgcgtaa agtcggttga aagtattcaa gaaaagatgg ttttacgtgg tatttcagaa
     9901 gaaaatcttg ctcaggactt gcaagatatt gcaggtcttc gtatcatggt acaatttgta
     9961 gatgacgttg atgaagtcct tgccttgttg agaaaacgtc atgacatgac agtggttcag
    10021 gagcgagact atattaccca tatgaaatct agtggatatc gttcatatca tgtcgttgta
    10081 gagtatcctg tagacacaat agatggacaa aagaaagttt tagcagaaat acaaattaga
    10141 actctggcta tgaatttttg ggcaaccatc gaacattcat taaattacaa gtatcagggg
    10201 gacttccctg aagaaattaa acaaagactg gagaaaacag caaaaatcgc tttagaattg
    10261 gatgaagaaa tgcgtaagat tcgcgaagat attcgtgaag cacagctttt atttgatcct
    10321 ttaaatcgaa aattgagcga tggtgtagga aatagtgatg acacagatga attttacaga
    10381 tagagcaaca cgtgttgcta taattgctaa cggaaaatat caaagcaagc gtgtagcctc
    10441 aaagctcttt gctgctttta aacatgatcc tgatttttat ttatcaaaaa aggatcctga
    10501 tattgtcatt tctattggtg gagatggtat gcttttgtca gccttccata tgtacgaaaa
    10561 acaactggat aaagtaaggt ttgtcggtgt tcatacagga catttagggt tttatacaga
    10621 ctatcgagat tttgaagttg acacattaat taataatttg aaaaatgata aaggtgaaca
    10681 aatctcctat cctattttga aagtaactat tacccttgaa gatggtcgag ttattcgtgc
    10741 gcgtgcttta aatgagtcta ctattaagcg tattgaaaaa acaatggtag cggatgttgt
    10801 tattaatcaa gtggtatttg aaaggtttag gggagatggc attttagttt ctacaccaac
    10861 cggtagtact gcttacaata aatcactagg gggggcagta ctccacccaa cgatagaagc
    10921 cttgcaacta acagaaattt ctagtcttaa taatcgcgtc tatcgtacct tggggtcatc
    10981 ggttatcatc cccaaaaaag atgctataga gatagttcct aaacgagttg gtgtctatac
    11041 aatttctata gataacaaaa cggtccatta caaaaatgtt accaaaatag aatatagtat
    11101 tgacgaaaaa agtattaact ttgtttcaac tccaagtcac actagctttt gggaacgtgt
    11161 taacgatgct tttatcggag aacctgaaca ttgaaatttg aatacatagc agaagaaagg
    11221 tgcaaagtta aaacactttt gaaaagtcat gatgtttcaa gggggttatt agcaaagatt
    11281 aagtacagag gtggtaagat ttttgtcaat ggtgaagagc agaacgctat tttcttatta
    11341 gagattggcg atgttgttac gatagatatt cctgacgagc cttctcacga aactttggaa
    11401 ccagtacctc acgatttaga tattgtttat gaggatgatc attttttgat tttaaataaa
    11461 ccttttggtt tcgctagtat tcctagctct attcattcaa atacaattgc taattttata
    11521 aaacactatt atgtgtctaa taactatgcc aatcagcagg tccatattgt gacccggttg
    11581 gatcgagata caagtggatt aatgttattc gccaagcatg gttatgccca tgctcgtcta
    11641 gataaacaac ttcaggctaa agcaattgaa aaaaggtatt atgcattagt atcagggagt
    11701 ggtgatttag cagatagtgg tgatattata gctcctattg cacgagatgt tgatagtatt
    11761 attacgaggc gagtccatga atcaggcaaa tatgcacata ctagttatca agtagttgct
    11821 cgttatggtg atgttagatt agtggatatc aaattacata cagggcgtac tcatcaaatt
    11881 agagttcact ttgctcatat cggctttcct cttttgggag atgatttgta cggaggacgt
    11941 atggatctag gaataaacag gcaagcattg cattgtcatt ctttgtcttt ttacgaccct
    12001 tttatgggaa aaataaataa acagacgcta gacttgacag atgactttga cagcgttatc
    12061 atggaattac attaatttag aaatagaagg ttttatatat atgcgttcct tatttggtgg
    12121 tttaagagaa aaagttgcaa gtgattatgt taagattgtt ttccctgagg gaagcgatga
    12181 acgtgtatta cgtgcagcag ctcgtttgaa atttgaaggt cttgtggatc ctgttatttt
    12241 aggtaaggct gacgaagttc acgattcttt ggcaagactt ggttttgttg atcaagatta
    12301 tagcgttatt gaccctgaac aatacgaaaa atttgaagag atgaaagaag cttttgttga
    12361 gattcgtaaa ggcaaagcta ctatggaaga tgcagatcgt cttttgaagg atgttaacta
    12421 ttttggggtt atgttagtaa aacttggttt ggcagatggt atggtatcag gggccattca
    12481 ttcaacagct gatactgtac gtcctgctct tcaaattatt aaaacaaaac caggaatttc
    12541 acgcacttca ggtgtattct tgatgaaccg tgaaaataca caagaacgtt atatttttgc
    12601 tgactgtgct attaacattg atcctaatgc acaagagcta gcagaaattg cagtaaatac
    12661 tgcggataca gccaaaatat ttgatattaa tcccaagatt gctatgctta gtttttcaac
    12721 aaaaggaagt gcaaaagcgc ctcaagctga aaaagttcaa gaagcagcta aaattgccaa
    12781 agatttatct cccgaattag ctgttgatgg agaattacaa tttgatgcag cttttgttcc
    12841 ggaaactgca gaaattaaag ctcctaatag tgatgttgct ggaaaagcaa atgtttttat
    12901 tttcccagac cttcaatcag gaaatattgg ctataaaatt gcacaacgtt tgggtatgtt
    12961 tgaagcaatc ggaccgatct tacaaggatt gaatgctcca gtaaatgatt tatcacgtgg
    13021 atcaagtgct gaagatattt acaaacttgc tatcattaca gctgctcaag ctattgatga
    13081 gaaatcaaaa taagttataa aacgagaagg atttttccta gctcgttttt ctatatacta
    13141 gaggaggagt atttatgata tcacgtaaag ttgcgcttgt gacgggggca tctgctggtt
    13201 ttggtgcagc tgttgttact aaattagttt cagatggtta tagcgttatt ggttgtgctc
    13261 gtcgaatgga taaattaaaa tgccttggag aaaagttttc agagggttac ttttatccac
    13321 tttaaatgga tattacaagt agagaatcgg ttgacaaagc tttagaaagt ctgcctaaaa
    13381 atttgcaatc aattgatttg ttggttaata atgcaggttt agcacttgga ttggataaat
    13441 catatgaagc tgattttgaa gattggatga ctatgattaa tactaacgtt gtaggattaa
    13501 tttacttgac acgatgcatt ttaccgaaaa tggtagaagt taatagaggg ttaattatca
    13561 atttgggatc aactgcagga acaattcctt atccaggagc taatgtctat ggcgcatcaa
    13621 aagcttttgt aaaacaattt gctctcaact tgcgtgccga tttagctgga actaaaatca
    13681 gagttactaa cctagaaccc ggtttatgtg aagggacaga attttcaact gtacgtttca
    13741 aaggagatca taaaagagtt gaaaaacttt atgaaggtgc gcatgcaatt caagcagagg
    13801 atattgctaa taccgtgtca tgggttgcta gccaaccaga acacattaat attaatcgca
    13861 tagaaataat gccagtcagt caaacttatg gacctcaacc tgtttatcgt gattaaaata
    13921 gtaaagacca ggaaacgctt taataatttc tggtcttttt atagataagt gttgtttttt
    13981 taacttaatc tgctataatg agttcagcca tttttttgac taatattatt taatcggagg
    14041 gttggcagaa atgcaacatg tcaatcattc ttcttttgat aaagcatcaa aagcaggatt
    14101 tattattgct ttaggcattg tttatggaga tattggtaca agcccactct atacgatgca
    14161 atcattggtt gaaaaccaag gtggtatttc tagtgtcaca gaatcgttta tcttaggttc
    14221 tatatcttta atcatatgga ccttgacact tattacaact atcaagtatg tgcttgtagc
    14281 tttaaaggcg gataatcacc acgaaggtgg tattttttct ttatataccc ttgttagaaa
    14341 aatgacacct tggttaattg ttccggctgt tattggaggt gcaaccttgt tgtcagatgg
    14401 agctttgacg ccagctgtaa ccgtaacttc agccgttgaa ggattaaaag tagttcctag
    14461 tttgcagcat attttccaga atcagagtaa tgttattttt gcgaccttgt ttattttact
    14521 gttacttttt gccatccaaa ggtttggaac gggtgttatt gggaaattat ttgggccaat
    14581 tatgtttata tggtttgcct ttttaggtat tagcggtctc cttaatagtt ttgcccatcc
    14641 agaagttttc aaagcaatta atccatacta cggtttgaaa ttgttattta gtccagagaa
    14701 tcataaaggt atttttattt taggatctat tttcctggcg acaacgggag cagaagcact
    14761 atactctgac ttaggtcatg ttgggcgtgg aaatatacat gtttcatggc cgttcgttaa
    14821 ggttgccatt atactttctt attgtgggca aggggcatgg attttagcta ataagaacgc
    14881 aggaaatgaa ttgaatccct tttttgctag tattccttcg caatttacaa tgcatgtcgt
    14941 tattttagct actttggcag ctatcatcgc ttcacaggca ctgatttctg gatcatttac
    15001 cttagtttct gaagctatgc gactaaaaat attcccacaa tttcgttcaa cttatcctgg
    15061 tgacaatatt ggtcaaacct acatacctgt tattaattgg ttcttatttg ccattacaac
    15121 ctctattgtt ttgcttttta agacttcagc gcacatggaa gcagcatatg gattagcgat
    15181 aacaattacg atgctaatga caactatttt actgtctttc tttttaattc aaaaaggagt
    15241 aaaaagaggt ttagtcttat tgatgatgat tttctttggt attttagagg gaatcttctt
    15301 ccttgcttca gctgttaaat ttatgcatgg aggctatgtt gttgttatta tagcagtagc
    15361 aattatattc atcatgacta tctggtataa aggtagtaaa attgtttcgc gttatgttaa
    15421 gttgttggat ttaaaagatt acatcggaca attagataaa cttagacatg atcatcgtta
    15481 tccgatttat catactaacg ttgtttattt gacaaatcgt atggaaggag acatgattga
    15541 taagtctatc atgtattcta tccttgataa gcgccctaaa aaagcacaag tttattggtt
    15601 tgtaaacatt aaggtcactg atgaacctta tacagctgag tataaagttg acatgatggg
    15661 aaccgacttc attgtgaagg tagaacttta ccttggattt aagatgcgtc agactgtgtc
    15721 acgctatctt cgtacgattg tagaagaatt acttgaaagt ggacgtctgc ctaaacaagg
    15781 aaagacatac agcgttagac ctgatagtaa agtaggagat tttaggttta ttgtccttga
    15841 tgaacgtttt tcaagcagtc aaaacctaaa acctggtgaa cgatttgtta tgctaatgaa
    15901 atcatctgta aagcattgga cagcaacacc aatacgatgg tttggtctac aattttccga
    15961 agttacaaca gaagtagttc ctttgatttt tacagctaat cgaggtctac caattaaaga
    16021 aaaaattgaa cttacaacaa ctggagattg attaataaaa ggcgctggaa ttaaaccagt
    16081 gccttttagt tattagtttt ctttttagag tgacgagtgg attatagttc aaaatcatcg
    16141 ttagagatag aagcaccaga cgtgacatca gaccatccaa cgatagcttg accggattct
    16201 tttaaatacc cctctaatag tggtttaata ttatggacat gttcaatata accaactagt
    16261 tgcccactat cattttctaa gcgtttataa gattgtacta gaatataatc ttgtgatgcg
    16321 ccaggtaaga gtaaatgtag ctctttcgct tttttctcta ttagactgtt ttgaacccat
    16381 ttgtctatag gatattcttc ttcaggataa atatcaaaag gatcttcagg tttatggttc
    16441 gaataaattg tttgaccgtc cttattatgc aatctaaaac gatatgggag atggttaaat
    16501 aagtcaaaat ttagcatata gtaaaactcg gtcattgttt tggagagttc cgagaacgtt
    16561 ttaatatcgt tagcctctgt ttttgagtta gtctgactaa tcgctaagta gttggagtca
    16621 attaccagat gaggtatgct agtttctata tcatggaatg caaagctacc gtcaaaatct
    16681 gcatgattga gagttgaaat atctgtaatg atttgaatag aaaacaaata attaaagtaa
    16741 gtctttaacg taataaagaa attattttca gattcatttg aggcaaaagc cactattttc
    16801 ttgggtatta gcatatgaac atgataacgg tattaagagt aaattgcaat tgttatgttc
    16861 tctgaagtgg tgattgatgg gtagagaata gataagaaag tgaaggcatt aatattcaaa
    16921 aaactctttg tttttcttag ctttatttga tataataaga gctgttgatt gtcctttgac
    16981 aactaatata aacaatttaa ttttaattcg aatgaattaa atggagcacc ttgatttagc
    17041 ggggtgctct attttatgat aataatcaaa gtaaggagat tttaatgact aaaaagggat
    17101 ggcgtattat gcctgccata attacaacgg ctatattagg tttttcaggg attcttattg
    17161 aaacatctat gaatgtcact tttccgcttt tgatgaaaga atttggggtt aaccccgctg
    17221 ttattcaatg ggtgacaaca ggaaatttgt tagctgttgc tgtgactgtg cctttgtcag
    17281 catttatgat taaaaattta tcagagagac aaatttttac attggcaaat gtgctttttt
    17341 tatttggtgt tttaattgat agctttgccc ctaatttagc tatattgtta gtagggagag
    17401 tcttgcaagg tgttgggaca ggattagccc tccctttatt gtttcatatc attcttacac
    17461 aaattccgat ggagcgtaga ggactaatga tgggagtagc tgctatggtc acacttttag
    17521 caccggcagt tggacctact tatggtggtg tcatttcagg aatgttagga tggaagatga
    17581 tttttatgct tctggcacca atacttatca tatctacctt tataggtttg gcttctattc
    17641 ccaaacgtca agtaagaatt aatgataaac tcaattttcc tgcctttgtc agtttaggta
    17701 ttggcttggc aacccttctt ttagctattg aaaagatgtc tattttttac ttattagtag
    17761 ctattgttag ttttgttatt ttttactatt taaataaaca gctagaattt ttgaatttga
    17821 atgtttttaa agataaagat ttctcaatct tattgtatgg cgtcctggct tttcaaatga
    17881 ttcctctagc actttcgttc ttattaccta acctcttgca acttgtttta catcaaactt
    17941 caaccaaagc tggtttgttt atgtttccag gtgcaatagc agtagttttt ttatcccctt
    18001 ttgcaggtta tctcctggat aaaattggtg catttaagcc aattatgata ggtatctccc
    18061 tttctttgat aggtttaatt ggtacagcta tattcattcc tgcgaagttt gttgtagtac
    18121 ttttagcctt tgatattctt actaaaattg gtatggggat tggagcaagt aatatggtta
    18181 cgacagcttt aacaaaacta aagccagcac agtcagcgga tggtaatagt atcttaaata
    18241 cactacaaca atttgcggga gcttttgcaa ccgcagtagc ctcacaaatt tttaccatcg
    18301 gacaagtagc tattccgaaa aatggagcta taattggtag tcaatttgca gttctattcg
    18361 ttatcgttgt tgttatctta gctattgtag gattaactta tcttcgaaaa agaaaagcaa
    18421 ta
//
LOCUS       DATRSG010000043        18202 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRSG010000043 DATRSG010000000
VERSION     DATRSG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18202
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.043"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..681)
                     /locus_tag="VBD89_001380"
     CDS             complement(<1..681)
                     /locus_tag="VBD89_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO7135903.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGNYGFMMKTPILGITNPFLNLLIVTTLLSFLVLFVKQIFQKVR
                     MRASFKTEE"
     gene            complement(810..2030)
                     /locus_tag="VBD89_001381"
     CDS             complement(810..2030)
                     /locus_tag="VBD89_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO7135904.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            complement(2217..3584)
                     /locus_tag="VBD89_001382"
     CDS             complement(2217..3584)
                     /locus_tag="VBD89_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO7135905.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIAGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(3731..4777)
                     /locus_tag="VBD89_001383"
     CDS             complement(3731..4777)
                     /locus_tag="VBD89_001383"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO7135906.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(4918..5514)
                     /gene="recR"
                     /locus_tag="VBD89_001384"
     CDS             complement(4918..5514)
                     /gene="recR"
                     /locus_tag="VBD89_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO7135907.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(5529..7574)
                     /locus_tag="VBD89_001385"
     CDS             complement(5529..7574)
                     /locus_tag="VBD89_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO7135908.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(7703..8395)
                     /locus_tag="VBD89_001386"
     CDS             complement(7703..8395)
                     /locus_tag="VBD89_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO7135909.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(8572..9330)
                     /locus_tag="VBD89_001387"
     CDS             complement(8572..9330)
                     /locus_tag="VBD89_001387"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO7135910.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(9511..10707)
                     /gene="tuf"
                     /locus_tag="VBD89_001388"
     CDS             complement(9511..10707)
                     /gene="tuf"
                     /locus_tag="VBD89_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO7135911.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(11059..12327)
                     /locus_tag="VBD89_001389"
     CDS             complement(11059..12327)
                     /locus_tag="VBD89_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO7135912.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSVINQGVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGA
                     HGWIVIGPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLK
                     DWRFYSLFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFI
                     GTIAVVGVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGL
                     GNSIEKLGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNS
                     MIALGIGAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDAN
                     EKKELIMKEAEEQYKPQEKNEKIINLDAFK"
     gene            complement(12433..15228)
                     /gene="ppc"
                     /locus_tag="VBD89_001390"
     CDS             complement(12433..15228)
                     /gene="ppc"
                     /locus_tag="VBD89_001390"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO7135913.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            15437..17236
                     /gene="pepF"
                     /locus_tag="VBD89_001391"
     CDS             15437..17236
                     /gene="pepF"
                     /locus_tag="VBD89_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7135914.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(17295..17684)
                     /locus_tag="VBD89_001392"
     CDS             complement(17295..17684)
                     /locus_tag="VBD89_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO7135915.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(17668..18192)
                     /locus_tag="VBD89_001393"
     CDS             complement(17668..18192)
                     /locus_tag="VBD89_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7135916.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
BASE COUNT         5884 a         3514 c         2885 g         5919 t
ORIGIN      
        1 ttcttctgtt ttaaaactag ctctcattct cactttttgg aaaatttgtt taacaaatag
       61 gacgagaaaa cttaatagag ttgtcactat caaaaggttt aaaaagggat ttgttattcc
      121 tagtattggt gtcttcatca taaagccata atttccccca gtcaagaggt taataaaaat
      181 caaagacata ttaatgagaa gcgttagttg tataatcaat tttggattta atttgtccaa
      241 attggactgt gtcatcagat gtagtaaact aaggacaaat aaagcaaaat gtccaataaa
      301 gaagaaaata ttagtcacat gtagaaactg gtaagggtaa aagtctgggt gaatcattgc
      361 caatactcca cctactactc ctaacaatgc gaaataatcc tttaatattc taggcctcgc
      421 taagagaacg acaaacatgc ctattcgaca atggtaaagg ggaagacttt cagataaagg
      481 caattttgcc cagaaaaacc aagtataaat cgtaattaac tgaagtccta aaaggatact
      541 ccaaatagtt ttatacaaag gcttatctct attaaccaaa gtcagccaca tcagaaaagg
      601 taagacagat aaaatcaaac tatggataag taaaggcatc tgcggaatct ctgttctgat
      661 attagaaaag aattctatca ttttagtatt atctttctat cattttttta ctagatactt
      721 agtatatcaa attatttgct ataagacaaa actatttttc aaagaaaaaa ctctttggta
      781 aaaccaaaga attttaaatt agatatatac tacacaactt tagttgttaa ttttcttaaa
      841 tatagagata acaataatcc gaataagaat aaaaaaccaa aaaccattaa tgtcaattga
      901 taggaatttc cccatgaata tgtctttgat aacaataggg gcccaaacag acctgctatt
      961 gcccatgctg ttaaactata accatgtaaa gtagctaatt cctttgttcc aaaaatatca
     1021 cttagataag caggtaataa ggaaaaacct gcaccataac aagtcattaa aatagacatc
     1081 gctattgcaa atacaatagc attgaatgac aaaaataaac tagaagtcat aataaagttc
     1141 acaataaata atattataaa ggtcaacggg cgtccaatgt agtcagagag acttgcccat
     1201 aacagacgtc caaaaccgtt aaatatccct agtaccccta ctaataaggc tgcagattct
     1261 gcggaatagc ctgctaaatc ttgtgccatt ggtgaagctg ctgatattaa acctaagcca
     1321 cacgaaatat taataaacaa ggtcaaccaa atgatgtaaa aggtttttga ttttatagcg
     1381 acgtttgctt ttaatccagt gataatttgt gaattcatag cattcttttt accatcgtga
     1441 gtcaaaatag ttattttttc ctgaggtggt tgtttaataa attgtgaggc aatcatcatg
     1501 acaaaaaaat atactaatcc caaaatataa aacgttttac ccacaccaat cctaatcagt
     1561 aaggattgtg caagcggact tgttactaaa gaagcaaagc caaatcccat aatagcgaat
     1621 cctgttgcta gtcccctcct atcaggaaac catttaataa tagtcgatac tggagtaata
     1681 taacctgaac caagtccgat tcctcctaaa ataccgtatg caacatacag taaccataac
     1741 tgctgagttt caatggctaa gcctgttaac acattccctg ctccatataa aatagcagaa
     1801 atcattccca ttatcctagg accaaaacgc tctactaagt gtcccataaa agctgcagac
     1861 attcctagac aaaaaatagc caaactaaaa gcgaatgaaa ctgatgaaat atcccaacca
     1921 gtctctgaga taattgggtt acgaaacaca ctccaagcat aagttgatcc tagcattaaa
     1981 tgtaaaacga ctccagaaac cgcaactaca taacgattta aatttttcaa ataatcctcc
     2041 taaaaacgaa cttaagtttt atttttaaaa atatagttcg caataaacag attatcaaaa
     2101 aactgaatat atcaccagtt ttctgcttag catatctatt ttcctcaata aaactgaata
     2161 ttattcgtga ttaaaaaaca aaaaagttag ggtaacttgt taccctaacc cctctattat
     2221 cctacttcaa gaatatcaac tattttagaa agattcattg agttagaacc ttttaataat
     2281 atttgatctt tctccttaag agtatctttt acttttgcaa gcaattgatc aaactgatcc
     2341 acttcttgat tctttttaaa gaaatacact ttaccaattg ggaacatctg gcttgctaat
     2401 tgagctaacc cctcaatatc ctggccataa caaatcaaag tatcaataga gtcaggtctg
     2461 atactcataa tcatttgatt atgtaaatcc acagattgtt ctcccagttc tttcatatca
     2521 gctaaaagag caatcttctt tccaccatca ttattgggaa tagctgaaaa ggtttctagt
     2581 atcaatcgca tagccgttgg gttagcattg taaacatctg ataaaatatc tgctccattg
     2641 gctgatttct tccattcggt gcgattacga gttagttgaa gattttctag agcatccact
     2701 atatcttctt ctgctactgc taataatttt ccaacatagg cagcgaccat cgcattagtt
     2761 gcattgtact taccaggtac tggtatacgt aattggtgct ccaaagcatt agttttaaaa
     2821 gttaaggaat gtttttcttc tttgagttct gtcacttgca attcttggtc atggccaaaa
     2881 cgaatagtca tctggtttgc tggaagatat ggatcaataa ttggatctcc cggtgcaatt
     2941 aatatgccat ctgaactcat accatctgtg atttgcattt tcccttctgc aatcttttct
     3001 cgactaccaa aaaattctaa gtgtgcttca ccaatcaaag taacaacagc aatacgtggt
     3061 tttgcaattt cagataacac atggatatct ccaagatgat cctgtcccat ctccaaaaca
     3121 attttttcag tatcttctgg catgtgtagc acagtatatg gtaagccaat ctcgttattg
     3181 tagtttcctt gtgttttata agtcttatat gtggttgata agattgcagc tatcatatct
     3241 ttagttgttg tcttaccatt tgagccagtt actgcaataa catcaacgtt catcttttca
     3301 atataatact gtgccaagac ttgaaatgct ttaagtgcat cagacactaa aagataagga
     3361 tgacccgcta tttccttctc tgaaatggtt gctattgctc cattatcaaa agccatctct
     3421 ataaactcat gtccatctct tgctcctttt aagggaagaa ataagtcacc ttcactgata
     3481 ttacgactat caaattcaat atttcccagg ggaacgtctt caaactctga tacttgattt
     3541 ttagcgccaa ctactttagc aacttcatgc aaactaagct tcatatcatt tccttttcta
     3601 ttatttagca acaatgttgt agatagtatg ctatccctta taaacgaata tactcctaaa
     3661 ttataacaca ctttcataac atatcataga tttgaaaaag ctcaactaag aaagacttag
     3721 ttgaactttt ttaaattaaa tgactctcac gtttttcaaa catttcttta gctaacataa
     3781 ctaacttttc aatcaaatca ctataagtta gccccatatt ttcccaaagc agaggataca
     3841 ttgaccactg agtaaaaccg ggcattgtat tcagttcgtt taagaagatt tgtccatctt
     3901 tcgtcaaaaa gaaatcacag cgtgataaac cacaagcccc gattgcttta aaagctttac
     3961 ttgcatattg acgcattgct tccatagttg cttcatcaac tttagctgga atatccatag
     4021 taattttatt atcaatatat ttggcgtcat agtcatagaa atcgacgtct ttaacaactt
     4081 cgccaggaaa agttgtctta acatcattat tgcctaaaat acctacttca atttcacgag
     4141 ctgtcacgcc ttgttcaatc aaaatacggc tatcatactt gagagctaag tcaattgcag
     4201 agcgaagtga ggattcatct gtcgcttttg aaatacctac tgatgacccc atattagccg
     4261 gttttacaaa aattgggaaa cttaaagttt ctaaagagag tttaatcgca tgttccaaat
     4321 catcaccctc aaaataagtt tgatatgcaa cctgaggtac acctactgtt gcaaggactt
     4381 gttttgttgt aattttatcc atagccacgc ttgaagatag aatattagtc ccaacataag
     4441 gcatccttaa aacttctaaa aatccttgga tagaaccatc ttcccccatt ggtccatgta
     4501 aaacggggaa aacaattgca ttatcatcat agatatcact tggacgaacc attttgtcta
     4561 aatcaacagt ttggtttgtc attaactttt catctgaaga tggcatttca tcaaattctt
     4621 gtgttttaat aaattgacct acttgcgtga taaaataagt tttaacaaaa aatttatcat
     4681 aattaatagc acgcatgacg ctttctgcag acagtacaga tacttcacgt tccgcagagc
     4741 gaccaccgta aagcaagatc aatgtttctt ttgacatatt tcttctcttt tctatctaat
     4801 ctaagattga ccttttaata ataccatatt ttaacttaac tttcaaaaat caatccactt
     4861 aaagttagta ttgtaaaaga aaaagttagg agattcccaa ccttttcttt ataacaatta
     4921 taactctgta cgattttcga ttgctcgaag caaggttact tcatctgcat attcgatatc
     4981 tgaaccaaca gctaaacctc tcgctaatcg tgtcactttg ataccagctg gttttaagac
     5041 tcgtgaaata tacattgatg tagcttcacc atcagcagtc gcattggtag caacaataac
     5101 ttctgtcacc tgaccatcca tcaatcgagt aattaaactt ttcaaattaa tgtcatcagg
     5161 gctaatacca ttcataggtg aaattaagcc atgtaagaca tgatacaaac cattatactc
     5221 ttgaattttt tccatagctg acacatcttt actatcttca acaaccaaga taacagactg
     5281 gtcacgcgtc ttatctgtac aaattaagca aggatcatca tcagtcaaat taccacaaac
     5341 agaacaatag gttaattccc gcttagcagc taataaattc ttagcaaatt cattaacatc
     5401 ttcatcactc ataccaatgg tgtaaaaagc taaacgtgtt gctgttttag tacctatacc
     5461 cggtaatttt gaaaagctgt caatcaattt agcaattggt gttgggtaaa gcattcaaat
     5521 gtcctctctt attgtcctgt gaactgtgaa atatattgat caatcatatc tcgtgcaact
     5581 aattgatggg attttgttgt atcagttgta acatgcggat acattaccgc tactgctatt
     5641 ttacgattag tatcgtaggc aatagcgttt aagttgtagg tagaaactgt ttgtccattt
     5701 atatttttag caaatgtttc agcggtacca gttttaccac taatggttgt cacattcccc
     5761 ctcattgacg taccagttgc atagccactt ccactattga caacgttata aaaaccttct
     5821 tgaataatgg ctaactcttg atctgagata gctatcttat ttagtgtttt aggagtgatt
     5881 gaacgaacca attgagcgaa cttattagaa tcattccctt catagatatc tgaaaccacg
     5941 tgaggtgcta aacgattccc attattcgca atagttgaga tatactgacc aagttgcatt
     6001 ggtgtatagg catcatactg accgaacgat tccattagag ttgttcctaa gctgtatttt
     6061 ccaggtacat aaccttctga ttcaggtaaa tctaatcctg tagaaacccc taaaccatat
     6121 tcgccgtaag ttttccttag tttagccatt gcttcttgat agcctcgatc agttaaagca
     6181 tctcctgtat tatagtcttg tcccattaaa cgaagtgcta cttgaaccat ataagtatta
     6241 gatgaatact ctagcgcttg tgccgcagat attggagtca aacctcgtgt aaaccatgac
     6301 cgtatattag caatttcttg gtcataaagc acttcatttc ctctaagaac tttattttcc
     6361 catcctgatg atagcgtcgc acctttcaca acagatcctg gcgtgaaatt cttagcaata
     6421 gtgcctaggc tgtcatcaac taaattccca tgatcatttt tcagcccagc cattgctaac
     6481 actttaccag ttgaaggttc tatagcaact gcatacatcc cttcggaaaa actagcgcgc
     6541 ccactagata attcactgcc atagtattgt ttcaagatac tttctacttt gttttggtaa
     6601 ttaagatcaa tcgttaattt aagatttctt cctgacttac cttcttgcgt aatattatct
     6661 gaaacaactt tacctttttt attaacaact actttgcgaa tttgtctgat tccttgtaag
     6721 tcatcttcat attgtttttc aaggtaagaa gttcccactc ggtcattcag tgaatagcct
     6781 tttttcaaat attttttaac ttcttctctt ggtaaacctg ccttttctgt agaaatagta
     6841 cctaatatat ccgataaaga agttcctttt ttacgacgat gccaattact tgttacagaa
     6901 attcctaaca gttccttctt atcagcatcc agtttcttga tttgatcatc acttaattca
     6961 cctgtactta atttcactga accaaacgtt ggtgtcgcat tcatttggtt aaataaggca
     7021 accactttta actcatcttc agaatagtta atagcgctca ctggaactgc agcaactgca
     7081 tttgcatata cggttgattc ggctagatgg ttaccaaatt tatcgtaacg tttagaatct
     7141 ggtaaacttt caacaacttt tttgtagttg gctttatctg ctaaataata atctctcttt
     7201 gctctatcgc ttgaagcaac ttcggttaag gtaatgtaat gtgataattt attcgctaac
     7261 tctttgatag actgagatga aatattgttt cctctagaaa acgccacagt aggacgttca
     7321 tcattttcaa ctaagacaac tcccttagca tcaaaaatct gacctctcgc ttttgattct
     7381 ttcactgtat aactcgtaag agctgtcagt tttttcatgt aaaatgattg ttgcccaatc
     7441 tgcatttgct ctaaacgaag aattagaaca gtaaataata aaacaataat aaaaaataga
     7501 agatttaatc gtctaggtat gctagcattt tgctttttta cagtgagacg ataccttttt
     7561 ttacgattca acattaactc cttgctataa aaactctgac tactattgta tcataaaaat
     7621 ggttttttac agacactcaa aatgttataa ataataaaaa attcaagcca acttggcctg
     7681 aattttttat ataaagatta gattatttac ctaagtaata ttctgaaaca aggtttaatt
     7741 tttcatcaaa ttcgaaaaca agtggtggga agttaggaat ttcaacgtcc atgatttcat
     7801 catctgacaa ttgtttgata tgttttacaa gagcacggat tgagttaccg tgtgcaccaa
     7861 caaacacatt tttaccatct ttaagagcag gagcaatttt atcttcccag aaaggaagag
     7921 cacgctctaa agtaactttt aggttttctg catctggaat aacagaatca tctagtgaag
     7981 catagcgacg atcagtatgt gctgaatgtt catcatcttt agccatatct ggaggcaata
     8041 catcatatga acgacgccaa atatgaactt gctcatcacc aaattgttca gctgcttctg
     8101 ctttattttt tcctgtcaat ccaccgtaat gacgttcgtt caagcgccat gatttttcaa
     8161 ctggtaccca aagttgatca gctgcttcaa gggcaaggtt agttgttttg atggcacgtt
     8221 taagaactga tgtaaaagca aggtcgaact caatacctgc tgcttgaatt aatttcccag
     8281 catcaatagc ttgttgtgta cctttttctg aaagatctac gtcagcccat ccagtgaaaa
     8341 ggttagcttt attccactca gattcaccgt ggcgtgcgaa tactaatttt accattagaa
     8401 aatttctcct ttaattttgg ctataccaat atttacttta actattctac aaaaaatagc
     8461 cataaaaatc tagtctgatt actagttcaa taatcaaaaa tagcttatta ttaactatct
     8521 ataagaaaag gcatatcacc tagagtgcta tgcctttaga tatcatgtga tttatttaac
     8581 aaaatctaat aaggcaagga agctttctgc ttcaagtgaa gctccaccta caagggcacc
     8641 gtcaacgtct gggcaagcca tatattctgc aacgttttca ggtttaactg aaccaccgta
     8701 ttgaacgcga actttgtcag caactgcttg accgaagtct gctgcaacaa cgtcacgaac
     8761 tgctttacac atgttttgtg cgtcgtcttg agttgctgat ttaccagtac caatagccca
     8821 gattggttcg taagcgataa caagtgatga tacttgctct tctgacaaac ctgcaagagc
     8881 tgctgaaact tgagcaccta cgaattcaac cgctttacca gcttcgtaag tttcaagaga
     8941 ctcaccgcaa caaatgattg gagtcaaacc attagcaaag atagcttttg cttttttgtt
     9001 gatatcttgg tcagtttcat ggaaataatc acgacgttct gagtgaccga taacaacgta
     9061 gtctgtaccc atttcagcca atactttagg gctgttttca ccagtgaatg cacctgaatt
     9121 ctcaaaatag ctattttgag cagcgatttt caattctgaa cctttagctg cttcaagaac
     9181 tgttgataaa gtaagtgctg gagcagcaat ccctgcttca acaagttctg atgatggtaa
     9241 tttagaagct actgcttcga tgaatgcttt tgcttcttct ggatttttat tcattttcca
     9301 gttaccagcg ataaatggtt tacgtgacat tacacatacc tcttcttttt ttatttacta
     9361 atctatcata ccataaatta tagttaatta aaagtttcac gagtctagta aaacgcttta
     9421 attatagata ctaaaaaaga gtccctgagg actcttcaca atttgagctg tctgactcta
     9481 actgccgttt gggaacttaa ttaagttaaa ttaagcttcg atttctgaaa cgatacctga
     9541 accaacagta cgtccacctt cacggattga gaaagtagta ccttgttcta cggcgattgg
     9601 gtgaatcaat tcaacttcga tagtaacgtt atcaccaggc ataaccattt ctgttcctgc
     9661 tggaagttcg attgaacctg ttacgtcagt tgtacggaag tagaattgtg gacggtagtt
     9721 gttgaagaat ggagtatgac gtccaccttc ttctttagaa aggatgtaaa cttcaccttt
     9781 aaatttagtg tgtgggttga ttgaacctgg tttagcaaga acttgaccac gttcgatttc
     9841 atcacgttga acaccacgaa gaagaacacc aacgttgtcc cctgcaagac cttcgtcaag
     9901 ttgtttacgg aacatttcaa caccagtaac aactgctttt tggatatctt ctttaatacc
     9961 aacgatttca acttcgtcgt tgacacgaac agtaccacgg tcgatacgtc ctgaagcaac
    10021 tgtaccacgt ccagtgattg agaatacatc ttcgactgga agaagtaaag gtttgtcagt
    10081 atcacgttct ggttctggaa tgtactcatc aacagtgctc atcaattcca tgatgatgtc
    10141 ttcgtatttt tcatcgcctt caagtgcttt aagagctgaa ccttggataa ctggaaggtc
    10201 atcacctggg aagtcgtatt ctgaaagaag gtcacgaatt tccatttcaa ccaattcaag
    10261 caattcttca tcatcaacaa ggtcaacttt gttcatgaat acgataaggt gtttaacacc
    10321 aacttgacgt gaaagaagga tgtgctcacg agtttgtggc attggtccat cagttgaagc
    10381 aactacaagg atagctccgt ccatttgagc agcaccagta atcatgtttt taacgtagtc
    10441 cgcgtgtcct ggagcgtcga tgtgggcata gtgacgtttt tcagtttcgt actcaacgtg
    10501 tgcagtgttg attgtgattc cgcgttcgcg ctcttctgga gcagcatcga tagaagcgta
    10561 atcttttggt tggttaactg aagtaggcaa gcgacgtgcc aatacagttg tgatagctgc
    10621 tgttaaagta gttttaccgt ggtcaacgtg tccgattgta ccaatgttaa cgtgtggttt
    10681 actacgatcg tatttttctt ttgccatttt aggaaaagcc tccaataaaa tatattttat
    10741 agatagacgg taggcaatac agtctaactt tacttatcta ttttaacaaa atcctctaaa
    10801 aaagcaagta ttttatgctt ttttgcttat ttctttattt ccttaaaatt cgggtaagat
    10861 tgattgaggc gtttgttatc tcttatatgt attttttagt agttatcata taatcatatt
    10921 gttaaataca tcctaattta tagtattaga atattaatag acacttcttt tcttcaaata
    10981 tcttctctct attaattaga gtttaaattt caaaaaatac aaaaatcgag ataaaaatct
    11041 cgttttttgt ttggatgatt atttaaaggc atcaaggttg attatttttt catttttttc
    11101 ttgtggctta tattgctctt ctgcttcttt cattatgagt tcctttttct cgttagcatc
    11161 aatattaagc acgaatccga tagctacaga aagtaccaag aggctgttcc ctccttggga
    11221 taagaaagga aatgtcactc cagttgaggg gattaaccca gaaataccac caatgttaac
    11281 aaaaacttgc atcaatagca ttgctccaat accaagagcg atcattgagt tgaaaggatc
    11341 tttagccttt atacctacgt gcataatacg taaaattaag aagaaaacca aagctaaaat
    11401 aaaccctgca ccaataactc ctaattcctc aataacaatt gaaaagacaa agtctgttgt
    11461 tgcttctggt aagtacccta atttttcaat agaattccca agtcctcgac caaaccagcc
    11521 accgttgctc attgcataat atgaattagc caactggtgt cctgaatctg ttaaatcttt
    11581 aaatgggtca aaaaaagcat taaatcgctt agctacataa ccaaagacag ggactttagc
    11641 cattgtctca actccaacaa ctgcaatagt tccgataaat aaagttgacc ccacgacgat
    11701 tagaccaagt aaagctgaaa accatcgata accaatacca ctaatacagt acataataat
    11761 gaccgttaga acaattatcg aaccatttcc taaatcaggt tgtgcaatta ccaaaccaat
    11821 cataaataaa gaataaaatc gccaatcttt taggtcgctt agtgatctcg gaagccatct
    11881 cccttttgtc aaagcttgat aatcatatat ttctatcttt ttttgccttc gagcaaaagt
    11941 aaacgctaaa taccatacaa tgataacttt taaatattca gcaggttgaa aactaatagg
    12001 accaataaca atccaaccat gtgctccatt aacttcttgt gtgaaaaagc gcgctattag
    12061 tagcaaaaat acttctacca atactgccat agtcaacact ttagaatttt tcaaaaaatt
    12121 tagtttcagt ttatagataa agataattgc aaccaaactg actgcccaaa agacaccctg
    12181 gttgattact gaacgaaaag ggttagctcc aagctgaatt agagttgcac tagttgtcga
    12241 ataaattaca attaagccta gaatagacaa tatcaaatag ggaatgagga tggaataatt
    12301 cagaagatgc cttttatcaa ttttcatatt gtattatttc ctttaatata tcaaaaaatt
    12361 accattatgc attatatcat ctttcgaagt aaagtaaaag ggagaatagc aatgatcctc
    12421 cctctatcaa tattaacctg aatttcgtaa acctgttgca ataccattaa tagtaatatg
    12481 aatcaacttc tcatagtctt catctagttg gttactacga agacgtttaa tcaattcaat
    12541 ttgaacataa ttcaaaacat tgaaatatgg caaacgataa tctaaacttg catgtaacat
    12601 tgggttttct tctaataaat tatcatgctg ttcaatagct aaaatcatat ctttagttaa
    12661 ctgccattca ttaaggatga tattaaaaac atctcgaact tctttacttc cagctaactg
    12721 agcatactga agcgcaatat tcatatttga ttttgataaa accatatcta cattagataa
    12781 aagagaatta aagaatggcc atttttgata catggtttgt aattttgcta agtttgcctc
    12841 atcttgttca ataaattgct tgaaggctga cccaacacca taccaacccg gaaacataat
    12901 acgattttgt gaccatgaaa atacccatgg aattgcacgt aaacctgaaa tttctgtaat
    12961 tgttttacgt gctgctggcc gagaaccaat atttaaactt gatacctctt taattggact
    13021 tgcttcaaag aaatagtcat agaaataagg attatcaaaa actaaatttc tataaacggc
    13081 attactttca gccacaatac catccattgt ctcacggaaa ttatcaattt cattaaggtt
    13141 agtaatcata cgtgttacca tgcggtctat ggaagctgaa ataagcattt caaggttata
    13201 ataggctgca tcttgattac catatttatt ctcaatgatt tcaccttgct ctgttaggcg
    13261 aattcgatct ttgattgaac caaaaggttg tgatgtgata gcttcataag atggaccacc
    13321 accacgacca acagttccac cacgaccgtg gaaaaatgtg attttaatac cgttttcttc
    13381 acctattttt gttagctcat tttgagcttt atatagagtc caaccagatg ataaatagcc
    13441 accatcttta ttactatctg aataacctag cattatttct tgataattgt tgttagttgt
    13501 aatccatttt tttactaatc cataatgtag atactgggtc ataatatcac gtgaattatc
    13561 caaatcttcg atggtttcaa ataacggtac aatctgaatg cgagcttgat tggcatcaat
    13621 tagtccaact tcttttaaca tgatagctaa ctcaaacata tccgaaacgc tctctgtgtg
    13681 agaaatgata tgctgtttta taatgtcttc acccaattga tcttttaact cacgtgctgt
    13741 ttggaaaata gccaattctt tttgaagtaa ctcggattta ggagtatgcg tagaagataa
    13801 tgttctagga tcttctgtta attcttttaa gagaagttga catttttctt cttctgaaag
    13861 agaactgtaa tcatctacaa tattggcaga cttcaataat tctgctacac aagcttcatt
    13921 aatactagaa tcttgacgca tatcaatagt agctaaataa aatccaaaga tatctactgc
    13981 ttgaataaga caagctaatt cgccatcgac aagagaatct tgtccatgtt gaactaaaga
    14041 ttgcttcaca agtaacaaat cttgtttaaa ctcctcagca gtagcgtatg ctggtagttc
    14101 ttctgtctgt atgtcagaag ctacttgagc gatatgtgat tttatatacc ttcctacaat
    14161 accagaagat tcttggcgac taacaaattt ctcttttggg taagaaccaa cttttaaatt
    14221 tagtagggtt tgtactagtt tatcttggat aaaattgaaa gctttacgat aaggttcatt
    14281 ttccctatat actgaattat cttgtgactg gtttgccaac gtaacaagtt cgggagagac
    14341 ttcagttaga cttattgaaa gtgacatatt acgataaagt tcatttagct gctcaatata
    14401 atggttaata atcacttcac tttgtaccat agctgataat cttagtgtct cagcagttac
    14461 aaagggattg ccatctctat caccaccaat ccacatcccc atagtcagag gcttaggatt
    14521 ctctaagtga ataccttttt tagctgccag tgccttatat tctgctgtta atttggtaac
    14581 tgctttaata agagaacggt tgtaatattc cattacattg gttatctcat ttttgacttt
    14641 caattttttc tcgcgaatag tgtctgtttg catgataatg ccgatgtaac gacgcaagtc
    14701 agcataccat ttttcttgat tcacgattcc tgctttgaca tctctgtatt tacgtaataa
    14761 atcatgaatc ttagacgtta attcgagcac cgttttacgc tgcacctgag taggatgagc
    14821 tgttagcaca ggcacaacat tgacgtgttc aagaatatct ttagcatttt catggccagc
    14881 tacaacatca atagtcgttg ataactttcc taggtaatct tggttaagat tatttttata
    14941 attaatttca tatgctaaat caacgtcttc tgaaatatta attaagagcg gcaaaataga
    15001 gaagtaacga gaaacaataa ccatctcctc gttgcttaaa gccgaaatag tttccttcaa
    15061 accagtataa tcgtcagttg atgataatga aactattttt tcaatcttgt caaatgattc
    15121 actaccaatc aatttttgcg ttgcttcatc caaaagctgc ttcagtaaac cgacctcttc
    15181 ggtaatgatt tctttattgc tactactttc taatttcggg tgggacaaac catcacttcc
    15241 tctcttaaat ctcactacta tgataccatt ttaaagtaaa aagtaaacca ttctaggatt
    15301 tttagataga attttcagat ttttacttat ttatgacagt aatcagtttt tcttgttcga
    15361 cttttttgat atactgagga taattgagat taactgatta attgttaatt aacaactatt
    15421 attaaaggag tatcgcatgg aacttaaaaa acgctctgaa ttccctgaaa atgagctttg
    15481 ggatctgact gctctctaca aagatcgtca agatttttta ctggctattg aaaaagctct
    15541 tgaggatatc aaggttttca aaaaaaatta cgagggaaaa cttaattgtg ttgaggattt
    15601 tactagtgcc cttatggaaa tcgagcatat ttatatccag atgagtcaca ttgataccta
    15661 tgcttttatg cctcaaacta ctgactttag caacgaagag tttgcacaaa tttctcaagc
    15721 aggatcagac ttcgcaacta aagctaatgt actactttct ttctttaata ctgccctagc
    15781 aaatgcagat ataaagattt tagattcttt agaaaataac cctcatttca aagcaactat
    15841 tcgccaagct aaaattcaaa agcaacactt attaagtcca gaagtcgaaa aagctttgac
    15901 aaacttaaat gaagtcctaa atacaccata tgatatttat actaaaatgc gtgctggcga
    15961 ttttgatatg gaagattttg aagttgacgg aaaaacctac aaaaatagtt ttgtaactta
    16021 tgaaaattac tttcaaaatc acgaaaatgc agagatacgt gaaaaatcct tccgttcatt
    16081 ctcgaaaggt ctacgtaagc accaaaatgc tgcagctgcg gcttatcttg caaaagtcaa
    16141 atctgaaaag ttaattgctg atatgcgtgg ttacgatagt gtttttgatt atcttctttc
    16201 cgaacaagaa gttgaccgtt caatgtttga cagacaaatt gatcttatca tggatgaatt
    16261 tggtccagta gctcaacgat tcctcaaaca tatcgccgat gttaatggta ttgaaaagat
    16321 gacttttgca gattggaaat tagacattga taacgaactt aatcccgaag taagtatcaa
    16381 tgatgcttat gacttagtaa tgaaatcagt tgctccttta ggaaaagaat attcccaaga
    16441 ggttgagcgc tatcaaaaag agcgttgggt tgattttgca gctaacgcta ataaagattc
    16501 tggaggatat gctgcggatc cttacaaagt tcatccctat gtactcatga gctggacggg
    16561 gcgtatgagc gatgtctaca cacttattca tgagattgga cattctggtc aatttatctt
    16621 ctcagataat caccaaagtt ttttcaatac tcacatgtcc acttactatg ttgaagctcc
    16681 atctactttt aacgaacttt tacttagtga ctacttagaa aaccaatttg atacagcacg
    16741 tcaaaaacgc tttgctcttg cacatcgctt aactgatact tacttccaca atttcattac
    16801 tcacttactt gaagctgctt tccaacgtaa agtttacact ctaattgagg agggagaaac
    16861 ttttggagca gaacaattaa atgctattat gaaggaagta ttaacacaat tctggggaga
    16921 tgctattgaa atagatgacg atgctgcttt gacatggatg agacaagctc attactacat
    16981 ggggctctat agctatactt actctgctgg tttagtaatc tcgacagccg gctatctcaa
    17041 tctcaaaaat aatccaaacg gagcaaaaga gtggttagca ttcttgaaat ctggtgggag
    17101 ctgtacccct ttagaaacag ccctattaat cggtgctgat atttctactg ataagcctct
    17161 acgcgatact attaatttct tatctaacac tgtagatcaa attattaatt atagtaatct
    17221 tattaaacaa ggctaaacta cagtctttca aaaacataaa aacaagttgg agatatctcc
    17281 aacttgtttt tatcttattt tttgtaaact tcctgataaa attcaatttt gtcgttcttt
    17341 ttaactttaa tttggtttgc tgccttagga gctaatttat tattgacctt gaacatccaa
    17401 taaagccctt tggtttcatc ttggctgatg ccatcaattg aagtaatgaa accatctttt
    17461 tctttaactt tatagttagc tttaagcaca tccaaaacag tgtcaccttt atggtaaaca
    17521 accttttcac ttaacacgtt cgtatcttct ttaacaacca attcaacctt accaacttct
    17581 aacgttgagc tagttgttgt cttattcgat gtagatggtt gcccgcaagc tactaaagtc
    17641 gtagcgaata ctgttagcaa gaataattta atgtattttt tcattcctaa atctccttaa
    17701 aatcggtaac aaaagaggat aaaataaaca agttgataaa gcgtgtgcca tattaaaact
    17761 aagtcctgct aatacataag tccaccaagg catatgatat aaccaagcaa aacaggtatc
    17821 aataatgacg ccataaacaa cccctaaccc tcctgcaaag aaagtttgaa gtactacctc
    17881 cgttatttta ccaaaacata cagcttttgt cagcggatat atcagaaatt tccacaagca
    17941 taaaattaaa gtgaaggaaa tgatttgcaa aaagacccat ggcccaaagc ccattaaaaa
    18001 agaagaaact agcattgtta cagttacagt agctacacct tcttttaaat cgaataagac
    18061 aactgtaatc aaaaaaatgg cagtaattgg ctgaatgtta ggcagaggcg caaaagcata
    18121 tctcaacaca acacacaaag cagataatat agctactcta gtcaatttag ttaaagttga
    18181 attctttatc atgtctttaa tt
//
LOCUS       DATRSG010000044        17826 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRSG010000044 DATRSG010000000
VERSION     DATRSG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17826
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.044"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(101..238)
                     /locus_tag="VBD89_001394"
     CDS             complement(101..238)
                     /locus_tag="VBD89_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135917.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFISTISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(297..503)
                     /locus_tag="VBD89_001395"
     CDS             complement(297..503)
                     /locus_tag="VBD89_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135918.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(654..1847)
                     /locus_tag="VBD89_001396"
     CDS             complement(654..1847)
                     /locus_tag="VBD89_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO7135919.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(1879..2853)
                     /locus_tag="VBD89_001397"
     CDS             complement(1879..2853)
                     /locus_tag="VBD89_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7135920.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(2968..3191)
                     /locus_tag="VBD89_001398"
                     /pseudo
     CDS             complement(2968..3191)
                     /locus_tag="VBD89_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3169..3561)
                     /locus_tag="VBD89_001399"
     CDS             complement(3169..3561)
                     /locus_tag="VBD89_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO7135921.1"
                     /translation="MVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLN
                     AEFEGAHLEYLRQNKLYLCKQDKIVTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMS
                     QTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(3550..3625)
                     /locus_tag="VBD89_001400"
                     /pseudo
     CDS             complement(3550..3625)
                     /locus_tag="VBD89_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793382.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
     gene            complement(3622..4713)
                     /locus_tag="VBD89_001401"
     CDS             complement(3622..4713)
                     /locus_tag="VBD89_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO7135922.1"
                     /translation="MTLRQVTLKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RHYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(4598..5530)
                     /gene="tadA"
                     /locus_tag="VBD89_001402"
                     /pseudo
     CDS             complement(4598..5530)
                     /gene="tadA"
                     /locus_tag="VBD89_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(5702..6067)
                     /locus_tag="VBD89_001403"
     CDS             complement(5702..6067)
                     /locus_tag="VBD89_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO7135923.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(6181..9831)
                     /gene="rpoC"
                     /locus_tag="VBD89_001404"
     CDS             complement(6181..9831)
                     /gene="rpoC"
                     /locus_tag="VBD89_001404"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO7135924.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(9948..13523)
                     /gene="rpoB"
                     /locus_tag="VBD89_001405"
     CDS             complement(9948..13523)
                     /gene="rpoB"
                     /locus_tag="VBD89_001405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7135925.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(14047..16344)
                     /locus_tag="VBD89_001406"
     CDS             complement(14047..16344)
                     /locus_tag="VBD89_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7135926.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            16455..17714
                     /locus_tag="VBD89_001407"
     CDS             16455..17714
                     /locus_tag="VBD89_001407"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO7135927.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT         5537 a         3657 c         2927 g         5705 t
ORIGIN      
        1 tactttgtaa tccttcggag atactttact attgggtgac aataatcctt ttattaacat
       61 agaatactac tttctaaaac ttcaatttaa tcatcagcta ttatatcgtt ttcttcaatg
      121 aagcatagcg ataaatagca aaaatagaca tcgcaatgct aatactaata gtactgataa
      181 aaacatattg ctggtaaacg attggtggac aaaaatcaat agtccacatc cttgacatat
      241 tacagattaa cttttaacct tttgtctttt gtggactgta tttttccatt aaaattctat
      301 cctccttcac tttctgtata aataaaaact tcctcaattt gtttatcaaa ataacgcgct
      361 atcttaaatg ctagctccaa agatgccgtg tatttctctt tttctagaga aataatagtt
      421 tgcctagtaa ctccaagtgc aactgcaagt tctgcttgac ttaatttcct agattttcgt
      481 aacttttgta gagaattttt catatcttac acctttttgt atagttagct ttacatttat
      541 agtatagtta actatacatt ttttgtcaag tacattttac atacctgttc aaaaaacaaa
      601 aaagttgata gttaaactat caacttttct ggtcttatca attttttgat aacttattta
      661 gctttaaggc gctcaacgtc acgcgcaatc attaattctt catcagtagg aataactaaa
      721 accttcactt ttgaatctgg ttttgtgata tctccaaaat aaccaaaaac atttttttct
      781 ggatcaagtt caatgccaaa ccatgataat cctgcgataa catcttgacg cattaatggc
      841 gcattttctc ccatacctgc agtaaaaata atagcatccg cgccatttaa tactgccaag
      901 tattgtccaa taaatttctt aatacggtca ataaagacat tataggctaa cactgcattt
      961 ggatctttac tttgaagacc tgcttcaata tctcgcatat cacttgaagt gcctgatact
     1021 ccaagcaatc ctgattgttt gttaagcata ttaacaacag cagctgcatc ttccaattct
     1081 ggatcattag ctacaagata agggataatc gcaggatcaa tgtctcctga acgtgttccc
     1141 atcattggtc ctgcaagtgg tgtgaatccc atcgatgtat caatagactg tccatgataa
     1201 tttgctgtaa tagaaacacc attacccaca tgtgctgtga ttaattttaa ttcttctaaa
     1261 ggacgtccta attgcttagc tgcttcttgt gcaacgtatt ggtggcttgt cccgtgtgca
     1321 ccgtatttac gtactttata atcagtatag tacttttgtg ggattggata taagtaggta
     1381 tgaggttgca ttgttgtatg gaaggctgta tcaaatacac atacactcgt gatatctggt
     1441 aagatttctc taaacgcacg aatccctgct gcagctgctg ggttgtggag tggagctaaa
     1501 gctgataatt cttcaacctg ttctacaact ttatcatcta ctaaagcaga ctctttaaaa
     1561 tattcaccac cagcaacgac acgatgacca acacctgtaa tttcattaaa gtctttgata
     1621 ataccatgtt ttgttagatc ttctaaaaga attttaacag cttgggtatg atctacgata
     1681 tctaaaattt gttcatcttt tttatcgtca aattttacag tcgaaataga atctttcaaa
     1741 ccaatacgtt cgataatccc tttggcaact actttctcct ctggcatttc atataattgc
     1801 catttcaaac ttgaacttcc agcattaatt gctattgttt ttgacataat ttacctctct
     1861 aaagcgtttt cacaatctct attctatcat attttttgag taatgactat tttctctgct
     1921 ccatttttga aaattttcaa tgaagtttgc catattctca cgattttgca aatctgtcag
     1981 cggatataca aaggtttctg gtttttgttc tgcttgcttc ttgagaacaa atatagattt
     2041 cgcattttga cgacttccaa aaatagtttc tggtagagtt aaaacggcaa tgacatctgc
     2101 atatcctttt aaccactcct tcagcaaatc actttgtgga cttgttaaaa ggttttcggg
     2161 tgctaaaaat atagcgattc catctttttt caaatattta agagattgct ccatcaatag
     2221 atggtgagca taggtgtgct ctttagaact tgatacagca tatcgtttag caatatcatc
     2281 attaggataa tagccaactg gtaaatcact aatgattaca tcgctttctt tcaaaatttg
     2341 tggtctaacg gcatcctctt ggataaattg ggcactagaa cctataattt cagcaatgct
     2401 tgctgataga tcaatcaaaa gatcatcaac ttcaatgccc atataattta actctttcga
     2461 gctgttattg aggagagtct gagctaaatt cccagttcca cttccaattt ccaagacatc
     2521 cactgtctct tgactcgtta attcttccaa aagaaataac aagataaaac caatactatc
     2581 tggcgtaaac tgatgattag cttgtaattg ctctgtttgc gcagatttga taaaaatgaa
     2641 ctggaaagtg cgacgccatt cttcttgact taagtcaagt tgacgtaatt tttggttatt
     2701 caccacaaca atatctaaat cacaacttga accaaggtaa taagagttct gttcaattaa
     2761 ggcatcataa atatgagttt ttaattggtt ctcaatcgtt tggatatttt ctaaaatcag
     2821 ctcataggct gtctcaattt tttcaaaatt catgatttcc tccagaattt atcataacaa
     2881 aaaagacagc atctgaaaag agaagcaaac aaaaatcctg tttttaagca aaaccttgtc
     2941 ttactaatat ttcatcactg tatattttta atgtttatgg cttgctttat tatcattaga
     3001 cttgagaggt acttggagag tgtctatttt acgataaaac ttatggttat agtttcctcc
     3061 cttatcctat gatactgtct ttctgttgcc aatattcttg ctagataaaa ctgtaagacg
     3121 agtgtaaata cagcagccaa tacaatagct tgcaaaagga tgccagcttt aagtttcttc
     3181 tttaaaatca tagtaaaatg tcctttttaa cccgtcctta aaataaaaaa caagttttac
     3241 catacttttg gtctgactca tttgacaatt gtctaaccca taaaccattg gttgataacc
     3301 tcgaccatta taacctgtct tacggaaatc atctttatta gatttgccaa aggttacaat
     3361 cttatcttgc ttacataaat aaagtttgtt ctgtcttaaa tattccaagt gagcgccttc
     3421 gaattctgca tttagttgct gagttaataa cacccattca gactgactgg aagaagacat
     3481 cactactatc tgttgagcca acaattttgt tagtccttga taaactagca aagctcctgt
     3541 gattgtaacc aatgctacca aacattctaa aatgtgtaaa tgctttaacc ttcttatcct
     3601 tacattttaa caataaattt ttcatgataa tatctccata ttttgataca ttggtaacag
     3661 cattgctgca taaatcataa caatgataag agctacaaaa ataaaaataa cgggttggat
     3721 taattgggtc gctctagcta attttgtaaa aaaatcctcc cacttctcat cagcatatat
     3781 atctaactct gttcctaatt tcgccttaac ttgaccatat tcaatcatta aactaagctc
     3841 agttaagaaa aacggatagt ctaatacttt ttggtgaaat gctttacctg atagaaaacc
     3901 ttcttccatg tcatatccta tttccctaaa aagtttggat ttctgatttt gcattacttt
     3961 aacaatttgg tccaattcaa caccttgact taataaattt ccccactcac gggcatagta
     4021 agcagttaaa taaagcttaa catatgatcc aactaaggga attgttgtta aaaaacaagc
     4081 tactttaatg cgcgacaatc gcttttgaat aatataaaat attaaactaa aaataagtac
     4141 aactgctaaa agtaataaga aaatattcgg cacatttgta atcagtctag ttgcaaaatt
     4201 attttctcct aattggggca ttaaataatg cctaaggcca atcataatta gcaccagaaa
     4261 agacaataat atcaatgggt aagtagctac ttctattact ttttttctaa ctaacaaaag
     4321 attagctaaa taagactcaa tctttagtag gctccttgaa atgttaccat gcaaatcagc
     4381 taatgcaacc tgtgtgataa cagtgtcaga aaaacctaac tctcctaaca tttttgataa
     4441 atcttttccc tctaataatg ccttattcat cctatctgta taacaatcag acaataactt
     4501 actcctcttt aagaaggtga ccatatcagt taaagaaaaa ccactagcaa aaagattatt
     4561 aaaaagttgg actactttcc gttgtttctt gagggataat tttttcgact tgtgcctgtt
     4621 tcttactgat atgtccttct tcagccaaga tatccacttg tctattccac ttgtctgatg
     4681 agtgtttttt aaagttactt gtctcaaagt caattaggct tcctcctcca attaaacgtt
     4741 gatatgctat taattttaga ctattttcta actcttgata gttaacccct aattctataa
     4801 gcctatcata gactccggga atacttttag catgaatagt agaaaaaacc attactcccg
     4861 ttaaacttgc acgaataaca gcacgggccg tcgcttgatc tctaatctct ccgataatta
     4921 aaatatctgg acgatgccgt aaagacagtt tgattaaagc atcataagtc attccaatat
     4981 cctcattcaa ttggagttgt aacatcttgt cattcttgat ttctaccgga tcttcaatcg
     5041 tgataatttg cttattttta aatacttctg aagctaattg atacatgaga gttgttttac
     5101 cactccccac agggccggaa aaaagatata gccctcttgc acacagtact tccttcattt
     5161 gctttatatt atcaaaccaa tattttaagt cctgatgacc tgaatacaaa gtacgaataa
     5221 ctaaagattc ttgaccacga taatctccca cacttgatag tcgtaatgaa accagtcttc
     5281 cctctgacag ttcatagtca caagaaccta attgacttcg tcttttttct ccaacgttca
     5341 tgcctgccac aaatttaaag tgactaataa gactagccat cctattaaac tcaaaaacat
     5401 caataaaccg cctttcatca tcaatacgca tatagaattc ataacaatca cctttgggaa
     5461 tgatataaat atcttgagca tttacttcta ctgcctgatg aatgacgtgc tttgctaatg
     5521 attgaaccat cccgtacctc cttaaccata tattcgaaaa taaaataaaa aaatctcacc
     5581 aattggtgag attttttcaa tgatttcttg aggggtagta acataaatat tatgaagcag
     5641 aggctgaaca aacctattat atccccataa aaactatcta acttttttaa actactttta
     5701 attacaaatt taaagaacat gccgatggca actcagctat cttattaaat acttcaaatc
     5761 gctcaataga ttcttcctta ggaatttctt gccattcttt caataacatt aaggaatgat
     5821 actgttggag gtactcatca cattcttcac accaacgttt ttcttttata ctccaaaacg
     5881 ttgccaacaa actagactta ctttgaaaat aaggccactt ttcctgtccc ctatcccact
     5941 cctcttgaaa atagagcaaa gcttctgata aggtatcata ctcagcaatt tcagtaatat
     6001 cttcctccca accttcaata aaccaccatg gttcccaatc accaaacatt tttacaactt
     6061 gatacataat acacttcctt tagagagtat tatataaaaa ataagaataa aataaaaaat
     6121 atctgctcat tccaaattct tgtaaaaaaa gaacctgagt taaactcaag ttctttttga
     6181 ttaatcttct gttgtaggtg ttgatacttc cgtaacttct gcatcaacag gagttccttc
     6241 aataatctca acttcattaa cagcaagtgg ttcaatatta cggtaacgag ccataccagt
     6301 acccgctgga atgattttac cgataataac attttcttta agaccaagta agtgatcttt
     6361 cttaccacga atagcagcat ctgtaagaac acgtgttgtt tcttggaagg atgcagctga
     6421 taagaatgaa ttagtttcaa gggaagcctt agtaataccc ataaggacag gacggcttgt
     6481 cgcagggatt ccaccagaaa taacaatatc tttgttagca tctgtaaagt ctgaaatatc
     6541 cataagtgta cctggaagaa gatctgtatc tcctggatcc atgacacgaa ctttacggag
     6601 catttgacga accattacct caacgtgttt atctccgatt tctacccctt ggctacggta
     6661 aactttttgt acttcagcaa gtaggtacgt ttcaacagat aaagtatcac gaacttcaag
     6721 aagacgtttt ggttggatag atccttcagt taaggccgca ccacgagcta cttcatcacc
     6781 aacttcaact ttcatacgtg ctgtaaatgg aacaacgtat tctccctcac ctgtctgacc
     6841 tttaacgaat actttcttag tacgtgttga agagtcttct tcaattgcaa cgacttcacc
     6901 cttaacttca gtaatgactg cctcaccttt agggttacgt gcttcaaaga tttcttggat
     6961 acgtggaaga ccttgtgtaa tatcagtatt tgaagcaaca ccaccggtat ggaaggtacg
     7021 cattgtaagc tgtgtccctg gttcaccgat agattgagct gcaatagtac caactgcttc
     7081 accaacctca acagcatcac ctgttgccaa gttaataccg taacagtgac gacaaacacc
     7141 atggcgagtg ttacatgtaa atactgatcg gatagttact tcctcaacgc ccgctttaac
     7201 aactttagca gccatatctt cagtaattaa ggtatccgca ccaacgagaa tttcacctgt
     7261 ctcaggatgt ttgattgact tcttagtata acgtcctatc aaacgctctt caagtgtctc
     7321 agtaacctct ttaccatctg taattgctgt gattgtcaaa ccgcggtctg ttccacagtc
     7381 atcttcacga ataataacat cttgggcaac gtcaaccaaa cgacgagtaa ggtaacctga
     7441 gtcggctgtc ttaagggccg tatcagtcat acctttacgc gcaccgtgag ttgagaagaa
     7501 catttccaaa acactaagac cttcacggaa gtttgataag attggcaatt ccatgatacg
     7561 tccgttagga gcagccatca aaccacgcat accggcaagt tgtgagaagt ttgagatgtt
     7621 accacgagct cctgagtcca tcatcataac gatagggttc tttggatctt gtgtctcaat
     7681 aagacgtttt tcaagtgctt ctttagcttc acgccatgtt gttgtaacag caacataacg
     7741 atcttcttca gtcatcaaac ctcgacggaa ggctttattg atatcttcaa ctctgtggtg
     7801 agctgcatca ataatctcag ctttattgtc aataactggg atatcagcaa tacccacagt
     7861 taaaccagca agtgttgaat gataatatcc aaggtccttc aaacggtcaa ggaaagcaga
     7921 agtttctgtt gtacggaaac gtttgaaagt ttctgcgata atattaccaa gatttttctt
     7981 cttaaaggga atattaatct ctaagttatc aataactgct tgaatatcct gacctggctc
     8041 taagaagtat ttatctggag ttttttctgt caagtttgca ttattaggtt caattaagta
     8101 cggtaaatct tcaggcatga tatcgttgaa gagaatctta ccaactgttg taaccatgat
     8161 tttatgtttt tgctcttctg tccaaggctt attcggcatt gaatcaacag cgataccaac
     8221 acgtgtatgt aaatgaacat aaccattttg atatgccata actgcttcgt catgatcttt
     8281 gaaaatcatg ccttcacctt cacgaccagc atcttccata gtcaggtagt aatttcctaa
     8341 aaccatgtcc tgagatggtg ttacgactgg cttaccatct ttagggttaa ggatgtgctc
     8401 tgcagcaagc atcaaaagac gagcttctgc ttgggcttct tcagacaatg gaacgtggat
     8461 ggccatttgg tctccatcaa agtcagcatt gtaggcctca catacaagtg gatgaagacg
     8521 aagcgcctta ccatcaataa ggacaggttc aaacgcttgg atacctaatc tgtgaagagt
     8581 aggtgcacgg tttagaagta ctgggtgctc tttaataact tcttctaaga tgtcccaaat
     8641 acgctcgtcc ccacgttcaa ccatacgttt agctgcttta acgttccctg cgaggtcacg
     8701 agcaacgatt tcacgcatta caaaaggttt gaagagctca atagccattt cacgtggtac
     8761 accacattgg tacattttaa gtgttggacc aacagcaata acggaacgtc cagagaagtc
     8821 aacacgttta ccaagcaagt tttggcggaa acgaccttgt ttacctttaa gcatatggct
     8881 taatgatttc aaaggacgac tacctggtcc ggtaattgga cgaccacgac gaccattgtc
     8941 gataagcgca tcaacagctt cttgtaacat acgtttttca ttttgaacga tgatgccagg
     9001 agcatttaat tccaacaaac gtgccaaacg attgttacgg ttaataacac ggcggtaaag
     9061 gtcattcaag tctgatgccg caaaacgacc accatctaat tgaaccatcg gacgaagatc
     9121 cggtgggata accggtaaaa tgttaagaac catccattct ggtttatttc cagatttttt
     9181 aaaggcatct aacacatcca gacgacgaac tgctttcaca cgtttttgac ctgtcgctga
     9241 tttcaactcc tctttaagaa ctgcgatttc tgcatctaaa tcaacgcgtt taagaagatc
     9301 ttgaatggct tcagcaccca ttttagcaac aaacgaaccg taaccgtact cttgaagttt
     9361 ttcacggtat tcacgctcag taaggagtga cttcggttct aatggtgtgt ccattggatc
     9421 gataacaaca taagccgcaa aataaataac ttcttcaagt gcacgaggac tcatatccaa
     9481 tgtgagtccc atacgcgatg ggataccttt gaaataccag atatgagata ctggtgcttt
     9541 taactcgata tgtcccatac gttcgcgacg aacttttgca cgagttactt caacgccaca
     9601 acggtcacaa atgattcctt tgtaacgaat acgtttgtat tttccacacg cacattccca
     9661 atcttttgtt ggaccaaaaa taacttcatc aaaaagtcct tcacgttctg gtttcaatgt
     9721 gcggtagttg attgtttcag gttttttaac ttcaccataa gaccatgaac ggaccttact
     9781 tggtgatgct aatgtgattt gcatactttt aaaacgattt acgtcaacca ctagttttac
     9841 ctttcttcga tttatcatca tactagccca gctaggattt caataaatcc taggactagc
     9901 attgcatatc aattctatat tcgcttctag ttataaacta gataaattta atcttcttga
     9961 acgacttcag atacttgttc agcttgtttt tcttcagctt cttgtttcac acgcgctttt
    10021 tcaaggtcat caacgtgcat cacatcatca tcttcacctt catcaagatc acggagttca
    10081 acttcgttgt catcttcatc aaggacacgc atatcaagac caagtgattg caattctttt
    10141 acaagaacac ggaatgattc tggaacacct ggttttggaa ttggcttacc ttttgtgata
    10201 gcttcataag ctttaagacg tcctgtaaca tcatcagatt tgtatgttaa aatttcttga
    10261 agaacatttg aagctccata agcttcaagc gcccaaacct ccatctcacc aaaacgttgt
    10321 ccaccaaatt gagctttacc tccaagtggt tgttgagtaa caagtgagta tggtcctact
    10381 gaacgggcat gaagtttatc atcaaccatg tggtgaagtt tgatcatgta catgacacca
    10441 accgacacac ggttatcgaa tggttcacca gtacgaccat cataaagaac agtcttagca
    10501 tctgaatcca tacctgcttc ttgaacagtt tcccaaaggt cttctgaaga agccccatca
    10561 aagactggtg ttgcaatatg aatacctaaa ttacgagcag ccatacctaa atgaagctcc
    10621 ataacttgtc cgatattcat acgtgatggc accccaagtg ggttcaacat gatatcaact
    10681 ggcgttccat ctggtaaata aggcatatct tcaacaggaa caatacgtga gacgacacct
    10741 ttgtttccgt gacgaccggc catcttatca ccgactttga ttttacgttt ttgagcgata
    10801 taaacgcgga caagcatatt aacacctgat tgcaattcat caccatttgc acgagtaaag
    10861 attttaacgt cacgaactac tccatcgcca ccgtgaggta cacgtagtga agtatcacga
    10921 acttcacgtg atttatcacc aaaaatggca tgcaagagac gttcttcagc agataagtcc
    10981 ttttcaccct taggcgttac cttaccaaca agaatgtcac cttcttttac ctcagcacca
    11041 atgcggataa ttcccatttc atcgagatca cgtagtgaat cttcaccaac atttggaatt
    11101 tcacgagtaa tttcttcagg cccaagcttt gtatcacgcg tttcagattc gaattcttcc
    11161 aagtgaacag atgtatagac gtcttctttc acaaggcgct cactcatgat aacggcatcc
    11221 tcgaagttat aaccttccca agtcatataa gcaacaactg ggttttgtcc aagagccatt
    11281 tcaccatttt ccatagatgg tccgtcggcg ataaaatcac ctttttcaac gagatcacca
    11341 actttaacta aggtacgttg gttataagca gtacctgagt ttgaacggcg gaatttttga
    11401 acatgataaa catcaagaga accatcttcg cgacgcactt caactttttc agcatctgaa
    11461 aaaataacac gaccgtcatg tttagcaatc acagctgcac ctgaatcgtg ggctgcttga
    11521 tactccatac cagtaccaac atatggtgct tttggatcaa tcaatggcac agcctgacgt
    11581 tgcatgttgg cacccatcaa ggcacggtta gagtcatcat tttccaagaa aggaatacat
    11641 gctgttgcga cagcaactac ttgcttaggt gacacgtcaa caaagtcaac aatgcttgaa
    11701 ggaaactctt ggttattacc ttgatgacga cccataacga tttcttctgc aaatgtaccg
    11761 tcttcgttaa gcttagagtt ggcttgtgca actgtaaatt catcttcttc atcagcagtc
    11821 aaccaaacaa tttcgttcgt taccgcacca gtagaacgat ctactttacg atatggtgtt
    11881 tgaataaatc catatttatt gaggtgtcca aatgaagata agttattaat caaaccaatg
    11941 tttggacctt caggtgtttc aattggacac atgcgcccat agtgggtata gtgcacgtca
    12001 cgaacttcat atccagcacg gtcacgtgtc aaaccaccag gtcctaaggc agagagacgg
    12061 cgtttgtgcg acaattctga tagagggttg tgttggtcca tgaattgtga cagctgtgat
    12121 gaaccaaaga attctttaac agcggcagtc actggacgaa tgttaatgat ttgttgaggt
    12181 gtcaagactt cattgtcttg tacagacata cgttcgcgga cattgcgttc catacgagca
    12241 agtccgatac ggaattggtt cgcaagtaat tcaccaactg cacgaatacg acggttacct
    12301 aaatggtcaa tgtcatctac cttaccgata ccctctgcaa ggtttaagaa gtatgacatc
    12361 tctgctaaaa tatctgcagg agttaaggca cgaactttat cttcaggatt tgagtttcca
    12421 acaatagtaa caacacggtc tggatcagtt ggagcaacaa ctttgaattt ttgaagaata
    12481 actggttcag taactacagc ataatcattt ggtgtgtaga caaacttgtt taagtctcca
    12541 tcgatatgct ctgcaattga atcaatgaca tctcgtgtca taacggttcc agcttcaaca
    12601 agaatctctc ctgtttcacc atcaactaaa ttttcagcga ttgtttggtt caataggcgt
    12661 gttttaagat tcagtttttt attaatctta taacgaccaa cagccgctaa gtcataacga
    12721 cgtggatcaa agaaacgtgc aactaaaaga ctacgtgagc tgtctgcagt ttttggttca
    12781 cctggacgaa gacgttcata gatttcttta agtgcttcat ctgtacgaga atcacttgga
    12841 tttttatgaa tgtctttttc aatagtattg cgaacaagtt cactgtcacc aaagatgtca
    12901 acaatttcat cgtctccaga gaaaccaagt gcacggacta gtgttgtaaa tggaatttta
    12961 cgtgtgcggt cgatacgtgt ataagcaata tcttttgcgt ctgtctcaag ttccaaccaa
    13021 gctccacggt tagggataac agttgaccca taaccaactt ttccattttt atctacctta
    13081 tcattaaagt aaacaccagg agaacggact aattgtgaaa caatgatacg ttcaccacca
    13141 ttgatgatga aagtacccat ttcagtcata attggaaaat caccaaagaa aacttcttga
    13201 gttttaattt caccagtttc tttattaact aaacggaaag taacaaaaat aggtgctgag
    13261 tagctagcat catggatacg agcttcttcc aaagtatatt taggttcttt taattcataa
    13321 ccaacaaatt caagatccat agtatctgta aagtttgaaa ttggaagtac atcctcaaat
    13381 acttctttca agccagcatc aagaaaatct tggaatgaat cagtttgaat ttcaattaag
    13441 tttggtaaat caagaacttc cttaattctt gaaaagctac gacgtgtacg gtgttttccg
    13501 tactgaactt catgtcctgc caagttttta gctccttttt aaagatagag tgactaagtt
    13561 tttatctcat aaaacttagt gagtttattt catttgctcg cctaacaaga acaagcttat
    13621 agatgtcgtt tgtggtaatt ttcagaaagt ttaaatatat attacaataa ccttttttct
    13681 taaaattagg cacaaaaaga gcagctaaat ctcactttat caagttgatt taactgctgt
    13741 atatctaggt cggacaatat ttcaacctag acagacgaca aatagttacg tacattataa
    13801 cttattttca ccaataaatc aagttttatt ctcttgactt caaggaagct tctagaattt
    13861 caatttgttc atcaacaccc ttgagtttta gcataacacg atgattttta aacatagcat
    13921 actggtgttt ttctaattct ttgaaagaag actatacaag aattaaatca tattatcttg
    13981 gaaaaaataa aaggagaaca gatcactcta ttctcctcaa ataaaactat cagtatcgta
    14041 aacctgttat cgttttccac ccaaagtaga ccaagctttt tgataatcac tatcagttcc
    14101 cccaatagca aaacgatagg tcatcgttcc aggtccattt tttgcccaat aacttgttgt
    14161 gctttcacct ccaacagtga ttcgacgacc gtttactgtt actacacctg gctgtaaacc
    14221 tgtagactta agaactttag cttttataac gctatcatca agtctaaacc tttctgactt
    14281 accaaaagta ttgccatctg cattattaat agcattaacc aagtgagcca tataattagc
    14341 attattattg taacctgtca atttcgctaa agaggcatta ttatcatgtc ctgcccatcc
    14401 acctaaagtc actttaggtg tagaaagcat caaccaaaca tcagaagttg agttagtagt
    14461 acccgttttt ccaatccaat ctaccccagc taaaccacta tttaaacctt gtaagcgatt
    14521 tttaaaggtt gtagtctttc cggagttaat tggtccatgt aatagctgct gtaagatagt
    14581 agctgttgct ttcgaaaaga cacggacagg tttactttcg tgattataaa tcactttccc
    14641 attactatcc tctatacttt ctatcatata ctgcttatga taaacaccac catttgcaat
    14701 catttgataa aggtttgttt gctgagcaac tgacgtatca ataccaccac caagtggtaa
    14761 actttctata ccaaagtttt caattggata gtctaatttc tccatatagt tttttacatc
    14821 aacgcctcga tctcgtagca tcttgtaagt ccagaaagct ggaatgttcc aagaaatgtc
    14881 caatgactct tgaaggttaa ccatagcagt accttcttca tccgcatgca taatcttttc
    14941 gccacttgaa tatgtcgttg gataatttga aagaacacta ccacttccta acatcccttg
    15001 atcaatagca attccgtaag gtaatatcgg tttaatactt gaccctggag agcgtgccgt
    15061 atcaaaagca tggttatttt gattctcact atagttacga ccaccaataa atcctataat
    15121 agcaccacta gaattgtccg ttagtacatt tcccatttga accttgccag taccatcatc
    15181 taacaaccct ccgtattgag ctgcagcatc ctgcatggct tgataaacag atttattgat
    15241 agtagtttta atagtatacc ctccctgttg aatctcttca atagcgcggt gtctgtacgt
    15301 cgctctagtc tcatcatttt ttaaatcatg ctctgaaaca ttatcctttt taatgaggta
    15361 attgtacatc actttttgag cttcagataa tgccgagtag taaagataat catgatgatt
    15421 tgttgtcgcc actgcaggtt tgataaaatc ttttttgatg tcataatctt tgtaggattt
    15481 gtactcatct ttggttaaag ctctcgttct atacatattg tataacacat ttttttggcg
    15541 ctttatacca aaactaagat ctttatctga tttcaactgg gcatctgcag tataaggaga
    15601 atatacaata ggactttgcg gtaaacctgc aaggaaagct gcctgaggaa ttgttaaatc
    15661 ttttgcggaa actccaaaaa tcccttgtgc tgcttcttca atacctgcaa tattctgtcc
    15721 tttgttattg cgaccaaacg gtgatacatt taaataatct gataaaatcg aatccttatc
    15781 catgtaacgc tctaatgcta gagcataaat aatttctttt gatttgcgtt taaatgacgg
    15841 atcatctccc aaaatttgct gctttaataa ttgttgtgtc agcgttgaac caccgctact
    15901 ctctccaaac cccagaacag atcccgctgc agcacgtagt acggctttgg gaactacacc
    15961 tttatggtca ttgaaatttt cgtcctctgt tgcaatgata gcctttttaa tattatcaga
    16021 tatagcatcc tttgccactg gtgtacgttg taaatcagtg gcaatctctg aaatttgtga
    16081 tttatcagaa taagttaacc tagacaccct agtaagtgta ttaacttgtg tcactaaact
    16141 atttttactt ggtactttaa cagagtcaac ttgactagca agatacccta ctgcaagtcc
    16201 tactccgagc ataacaaata gtaaaataat aacatagatg aaatctgata aaagctttac
    16261 aattcttaaa aaaatagaac caaattcaag tggtgtgtaa tcacctaatt tagaactatt
    16321 caacttctta ttacctttaa acatgaataa tacctcatcc tacattatat cagatttaat
    16381 ataacttgca tatcctaaag agttttgata tagttgacat taatacacaa caagttaata
    16441 attaggagaa aaagatgaat atttttgatg aactcaaaga acgtggactc gtttttcaga
    16501 caactgacga agatgcttta cgcaaagctc ttgaagaagg ttctgtctct tactatactg
    16561 gctacgatcc tacggctgac agccttcatc ttggacattt agttgctatt ttaacctctc
    16621 gtcgcttaca acttgctggt cacaagcctt atgcacttgt gggaggcgca actggactta
    16681 ttggggaccc ttcatttaaa gatgttgagc gcagtttaca aacaaagaaa actgtagtta
    16741 gttggggtaa taaaatccgc ggtcaattat ctaattttct agagtttgaa acaggtgata
    16801 acaaagctgt attggtaaat aattatgatt ggttcagcaa tattagtttc attgatttct
    16861 tacgagatgt tggtaaatat tttactgtca attatatgat gagtaaagaa tctgttaaaa
    16921 aacgtatcga aacagggatt tcctatactg aatttgctta tcaaattatg caaggatatg
    16981 acttttacga attaaataag aactataatg tcactttgca aattggtggt tctgaccaat
    17041 ggggcaacat gactgctgga accgaactta ttagacgaaa atcaaatggt gtatctcatg
    17101 ttatgactgt tccattgata acagattcta caggaaaaaa attcggaaaa tctgaaggaa
    17161 atgctgtttg gcttgatgct gacaaaacct ctccttatga aatgtaccaa ttttggctca
    17221 atgttatgga tgctgatgcc gttcgcttcc ttaagatttt tacattccta agccttaaag
    17281 aaatagaaga tatacgtatt caattcgagg aagcacctca ccaacgctta gcacaaaaaa
    17341 cactagcacg tgaagttgta actcttgttc atggtgaaaa agcttataag gaggccgtta
    17401 atatcacgga acagctattt gctggcaata taaaaggatt gtctgttaaa gagctcaaac
    17461 aaggtttacg tggagtacca aattatcacg ttcagacgga agataacctc aatatcatag
    17521 accttctagt cacttcagga gttgtcaatt ctaaacgtca agctcgagaa gatgtctcca
    17581 atggagctat ttatatcaat ggagatcgta ttcaagattt agaatatact atctcagaaa
    17641 atgataagct tgaaaatgaa attacagtca ttcgtcgtgg aaagaaaaaa tactttgtat
    17701 taaactttaa atagacttga agtgtaatcg atactctgat aataatattt caagacaata
    17761 aaaagtgtag tcaattgact acacttttta ttaatgaaca acattcctga agagacaact
    17821 tttaaa
//
LOCUS       DATRSG010000045        17766 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRSG010000045 DATRSG010000000
VERSION     DATRSG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17766
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.045"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            85..1038
                     /locus_tag="VBD89_001408"
     CDS             85..1038
                     /locus_tag="VBD89_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO7135928.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            complement(1088..2983)
                     /locus_tag="VBD89_001409"
     CDS             complement(1088..2983)
                     /locus_tag="VBD89_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO7135929.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFK
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            3131..4114
                     /locus_tag="VBD89_001410"
     CDS             3131..4114
                     /locus_tag="VBD89_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7135930.1"
                     /translation="MTEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDIIEAGK
                     TENALQYEVADMEEAISGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            complement(4160..5323)
                     /locus_tag="VBD89_001411"
     CDS             complement(4160..5323)
                     /locus_tag="VBD89_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7135931.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFLKRLFFKKM"
     gene            5435..6079
                     /locus_tag="VBD89_001412"
     CDS             5435..6079
                     /locus_tag="VBD89_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO7135932.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDITVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGC
                     FHLELSSLADSFGISYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            6145..6759
                     /locus_tag="VBD89_001413"
     CDS             6145..6759
                     /locus_tag="VBD89_001413"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7135933.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(6831..7832)
                     /locus_tag="VBD89_001414"
     CDS             complement(6831..7832)
                     /locus_tag="VBD89_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7135934.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(7912..9816)
                     /locus_tag="VBD89_001415"
     CDS             complement(7912..9816)
                     /locus_tag="VBD89_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO7135935.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDNPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQSLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVNCPGEHVLTNQYILDDQVIYENGL
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVHMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            complement(9896..10711)
                     /locus_tag="VBD89_001416"
     CDS             complement(9896..10711)
                     /locus_tag="VBD89_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7135936.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNLHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTAQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            complement(10698..11564)
                     /locus_tag="VBD89_001417"
     CDS             complement(10698..11564)
                     /locus_tag="VBD89_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7135937.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKTALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            complement(11600..12094)
                     /locus_tag="VBD89_001418"
     CDS             complement(11600..12094)
                     /locus_tag="VBD89_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO7135938.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            complement(12149..13345)
                     /locus_tag="VBD89_001419"
     CDS             complement(12149..13345)
                     /locus_tag="VBD89_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO7135939.1"
                     /translation="MMKIKPVKVESIENPKRFLDSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDAKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKHLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNSEFIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            complement(13348..13782)
                     /locus_tag="VBD89_001420"
     CDS             complement(13348..13782)
                     /locus_tag="VBD89_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7135940.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            14060..14872
                     /gene="dhuD"
                     /locus_tag="VBD89_001421"
     CDS             14060..14872
                     /gene="dhuD"
                     /locus_tag="VBD89_001421"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO7135941.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            14889..15527
                     /gene="dhuI"
                     /locus_tag="VBD89_001422"
     CDS             14889..15527
                     /gene="dhuI"
                     /locus_tag="VBD89_001422"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO7135942.1"
                     /translation="MKIALINENSQASKNTLIYKELKAVSKKKGFDVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADSVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     VKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            15553..16560
                     /locus_tag="VBD89_001423"
     CDS             15553..16560
                     /locus_tag="VBD89_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO7135943.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            16572..17210
                     /locus_tag="VBD89_001424"
     CDS             16572..17210
                     /locus_tag="VBD89_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO7135944.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDAETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
BASE COUNT         5752 a         3470 c         2916 g         5628 t
ORIGIN      
        1 ttaaattgat agaatttaag aatatcgttg ctcattatct ccatatgtgg tagactaaca
       61 acaatttaaa taggaggtta accaatgaaa aaattaacaa aaggcagcca cattcgtgtt
      121 cttagtccct cttcttccat tgaacgtctg ggtggttttg aggctaacct attagccaaa
      181 gaagtactag aaaagctggg atttcaagtg tcattctcta agcactatct agaaaatgat
      241 atcctctatt ctgcttcgat tgctagccgc gtagaagatt tacatgaagc atttgccgac
      301 ccttctgttg atgccatatt agcaaccatt ggcggattca attccaacga gcttttacca
      361 tatttagatt atgatttaat ctctaagaat cccaaaataa tttgtggtta ctcagattca
      421 accgccttcc ttaatgctat ttttgccaaa gcaaaaatcc aaacttacat gggccctgct
      481 tattcttctt tcaaaatgaa ggaaggtcaa ccttaccaaa cacaagcttg gcttactgcc
      541 atgacagaaa accactatga actttggcct agtgaagaat ggtctagtga tccctggtat
      601 gatcccagca aaccacgcca atttttccca acagaatgga aaatttataa tcacggaaaa
      661 gcttccggta cgattatcgg aggtaatctc tctacgtttg gcctacttcg aggtacccct
      721 tatgcaccga aaattgaacg ctatgtcctc ttgattgaag aggctgagga aagtaatttt
      781 tatgaatttg accgcaacct tgctgcaatt ttacaagctt atcctcatcc acaagctatt
      841 cttatggggc gctttcctaa agaatgcggc atgactccac aagtctttga atatatcctt
      901 tcaaaacatg ctattttcaa ggaaattcct gttatttatg atatggattt tgcgcacacc
      961 caacctcttt tgacagtgac tattggagct gagataagcg ttgacactac tacattatct
     1021 ttatctataa aagaataaaa aaatactcct gctactcatc tgagtaacgg gagtttatca
     1081 tttagagtta ccaaataata acacgttttt caactggacg ccacatgtta tcaccatctt
     1141 taacatcaaa ctctttatgg aattcttcaa aattcgttac tgtaatatta gttcgccatt
     1201 gtgctggggc gtgaacatct acactagcta acatttgcat atactcatca cgtgccttca
     1261 tacgccaaat tgtcgcaaag ttaataaaga aatccctcgc tgaaaaatct gactcacgct
     1321 gcgcagcttc taaagcacaa gcaactccac ctaaatctgc aacattttct gatactgtta
     1381 gtttaccatt gacttttgcg ccataagatt ccagtccatc aaactgctca actactttat
     1441 ctgtaagttt tttgaaagct ttaaaatctt catcagtcca ccaattattc aaacttccat
     1501 gttcatcaaa tgaagctcca ttagtgtcaa aagcgtgtga tatttcatgt gcaatcactg
     1561 caccaattcc cccataattt gctgatgaac tttgctctaa agcataaaat ggtgcttgta
     1621 aaatggctgc tgggaagaca atttggtttt gttgtggatc ataatacgca ttgaccatgt
     1681 gggcaggcat atgccattca cttctatcaa ctggtttatt ccatttactc caaccatagg
     1741 ctattgaaat tttgtctaaa ttagtagcat tttcgacaag cgatagttta ggatcaatga
     1801 tttttttagt ataagtctct ggtagtttct cagggtatcc aatgtgtggc gtgataacat
     1861 taagtttcac aatagctttt tcccgtgttg actgtgcaag ccaatccgcc ttttctaagc
     1921 gtgacttata gacatcaatc atcgttgcaa tcttatgctc aacatcagct ttagcttctg
     1981 gagagaattt ttcccctgca taccacaaac ctagagcttg attgtaaggt cctgaagcaa
     2041 ggtagtaagc tgcttttttc ttatccattg cttgtggtgt gcctgataaa gctcgactgt
     2101 aaactcccga tttgatacga atatcatcag ttaagtaagc attataggca ttcgcagctg
     2161 ataaaataag atctgccttt aataattccc aattagattc tgagtaatag tcagatgcaa
     2221 attctgtcca gaaacgttct tcaggaacaa taactttatc tggtttttgt cctaatatct
     2281 tttggaaaat agcatctaat ggaagttcag gtgctaattt tgtgaaatct tcccagttat
     2341 aaggatggta aagtttaaca tattccgaac tctcttcgcg agataagaca tattgtgcca
     2401 attttgcatc caaagctaaa accttatcca agatatcttt aatttcctgt tctgaaaggc
     2461 caaacaaagg cagtaagtct tcttgacttt tacgccaaag agcaaggagt tcctttcctt
     2521 tttcattatc ttcactataa taagttgtat ccggtaaaat aatcccaggc gcttcagccc
     2581 aaagtacatt caattgagca ttcataaagt caggagccac accaaatgga aattcatttg
     2641 gtttaccttc tagttcatat tctgcaactt ttgatgcaaa ctcagagaaa gattgtagac
     2701 ctttatattc ttcaattaat ggtaatactg gtgatactcc tacctcttca cgtttttgat
     2761 aatcagctgt cattttatga aaagcaataa aatgatttaa aatcgtatca cttggtttat
     2821 tttcatcagc cagccactta tctgtcgttg acagcattaa tgcttcaatg tcatcagcaa
     2881 gatcagaaaa accacctgtc cttggtttgt catcaggaat aacagctgtc ttagcccatt
     2941 ccccattaac cgcttggtaa aaatcatctt gatattttat cattattttc tccttaatat
     3001 ctcatatgta atctcttaca gtctaacaga aaattcagag tttgactatt aaaaatactc
     3061 tcatattggt gctttcttta aatatcattt tgttattttt gctatactat aaaccaacaa
     3121 ggaggttgtt atgactgaaa aatattataa ttgggcaacg cttggaaccg gcgttattgc
     3181 caacgaatta gcccaagcac tggaagcacg tggacaaaaa ttatattctg tagctaatag
     3241 aacttacgac aaaggacttg aatttgctaa caaatatggt atccaaaaag tttatgatca
     3301 catagatcaa gtatttgaag accctgaagt ggatatcatt tatatctcta ctccccacaa
     3361 tactcacatc tcatttttac gaaaggcttt agcaaatggt aagcacgttc tttgcgaaaa
     3421 atctattact ttaaatagta ctgagcttaa agaagccata gatttagccg aaactaacca
     3481 tgttgtctta gctgaagcca tgactatttt tcatatgcca atttaccgcc aattaaaaac
     3541 attagttgat agtggaaaat taggaccgtt aaaaatgatt caaatgaatt tcggaagtta
     3601 taaagaatat gatatgacta accgtttttt cagtcgtgac ctagcaggcg gtgctttgct
     3661 ggacattggt gtttatgcac tttcttgtat tcgctggttt atgtcagaag cacctcacaa
     3721 cattacctct caagttacat ttgcaccaac aggggttgat gaacaagttg gtatcctact
     3781 aaccaaccca gcaaatgaga tggcgactgt cagccttagt ttacatgcaa aacaacctaa
     3841 acgagcaact atcgcttacg ataaaggcta cattgaactt tttgaatatc cgcgaggaca
     3901 aaaggcagtt attacttata ctgaggatgg gcatcaagat attatcgaag ctggcaaaac
     3961 tgaaaatgct ctccaatatg aggtagctga tatggaagaa gccatttcag gaaaaactaa
     4021 ccacatgtac ttaaactata ccaaagatgt tatggatatc atgacacagc tacgtcaaga
     4081 atggggattt acctacccag aagaagaaaa atgaagagag cttgacaatg attgtcaagc
     4141 cctttttagt taatatacac tacattttct taaaaaatag gcgtttaagg aaataaatca
     4201 taccaactgc taaacttaaa atagcataaa catagaaaac tgcatttatt tgtcctagta
     4261 aatgatcaaa acaagacaga agaatagccg cactagctcc accaatatta caaaatacta
     4321 aaactaatgt cgttgcagaa ccaaccaacg aatattcaac acggtctgct actaggctaa
     4381 acacagcagt gaccatcagg ctgtaaagga aacctgacgc catcgcacta attgttagta
     4441 ccattagatt agaaggcaat ccaactccaa gcaatgttaa agaaaataag acgaggcaga
     4501 ttggaattaa ttgtttatga aacatagcta tcaactgacc aaaactcata cctgctatta
     4561 ttcccataag catcatcgca ctaagaacaa gactagcttg agctggcgta cccaaaccaa
     4621 attctactac tagcagaggt atcctaagat taatcccagt atttatagtt atgacaaatc
     4681 ctgctaaaat agctaaggcg acaatataag tcaaatcagc tttagggatt ttttgacctt
     4741 tagttttaat cttgtcattt gtatctttca ccactttctt aggcgcaaaa acaagatata
     4801 gaactaagat tggtaaagct aaaaaataaa tcataaatga tttagaccaa ccaaaagtca
     4861 acaatagccc aactaaggct gtcaaaccag catttcctaa aacttcaaag gaccccctta
     4921 atcctaagag ttttcttctt tctttgcctt gataatattc actaatcaca ctaatggccc
     4981 tagcattaat aaaaccgata ccactcccca ataaaagacg cgatacaaat actaaaaggt
     5041 agttctgact gacaaccggc aatcctcccc ctaatgctgt tagcaatagt ccaaagataa
     5101 tcatatgttt ctcagaaagt tttttactta accaaggtgt gataagcaag atgaagataa
     5161 tagccatcga aggaattgaa aataataatt caacttgggg tgatggtaag cccttatctc
     5221 tataataaga tatcatttgt ggtagggctg gtgatgtcga aaaagttgat agcaagataa
     5281 gtgataaaga gagtaaactt agcttctcca taaagatttt caagtgacaa tacctctttc
     5341 tgatgtcttt ttttagataa ataaagtata atcaatatta aactagaaat atatgacata
     5401 tgtcctaaaa ctatctcaaa tagggagtca atacatggat atagaaaact actgccaaga
     5461 ctactgtttg gagactatct ttccaaaaca ttatttcaag gatttacaag taatcaccct
     5521 aactgccggc caatccgtct gtaaacaggg agaacaatta gaatacttgc actacatcgt
     5581 caagggacgt tttaaaattg tacgtcgact ctttaacggc aaagaacata ttttagatat
     5641 taaaacaaaa ccaacactga ttggtgatat tgaacttttg actaatagac aaattgtatc
     5701 atctgttatt gccttagagg atattacagt cattcaatta tcacttaaag gacggaaaga
     5761 gaaactgtta acagacgcaa ctttcctcct aaaacttagt caagagctag ctcaggcttt
     5821 tcatgatcaa aacatcaaag cgtctactaa tttagggtat accgtcaaag agttacttgc
     5881 aagtcatatt ttagcaattg aagaacaagg ttgctttcac cttgaactaa gtagtttagc
     5941 tgattcgttt ggcatcagtt atcgtcattt attaagagtt atccatgata tggtcaaaga
     6001 aggccttatc caaaaagaaa aaccaaaata ctttatcaaa aatcgtttcg cactagaatc
     6061 attaaatatt cagccatgaa gcgtagcttt atcatttaat tattttatgc taaactaaat
     6121 cagagaatac agttaggaga aaatatgtct gctattgata aattagtaaa agctagtcat
     6181 ttaattgaca tgaatgacat tatccgcgaa ggcaatccaa cccttcgcaa agtggctgaa
     6241 gaagtcacat tcccactttc tgagaaagaa gaaattctcg gagaaaaaat gatgcaattt
     6301 ttaaaacact ctcaagatcc aattatggct gagaagctag gcttacgtgg tggtgttggt
     6361 ttagccgcac cacaattaga catctcaaaa cgcatcattg ccgtccttgt tccaaacgtt
     6421 gaggatgctc aaggaaatcc acctaaagaa gcttatagcc tccaagaagt aatgtacaac
     6481 cctaaagttg tatcgcattc tgttcaagat gctgctttat ctgacgggga gggatgccta
     6541 tcagtagatc gtgaagtacc tggctatgtc gtccgccacg cgcgtgtaac tattgaatat
     6601 ttcgacaaaa ctggtgaaaa acatcgactc aaattaaaag gttacaactc tattgttgtt
     6661 caacatgaaa ttgaccacat tgatggtatt atgttttacg accgtatcaa tgagaaaaat
     6721 cctttcgctg tcaaagaagg cttacttatt ttagaataag gacaaataac ctagagataa
     6781 ttttctctag gttttatttt ttaatagcaa ataaaataag aaatcttttt ttaaaaagtc
     6841 gactctttcc aaaacatctg gcaatctaca atctgttggt tatcgacgga gtcatctcct
     6901 tcaatacgat taatcaaaat ctgtgctact tgttcacctt cttcataagc cggctggaca
     6961 attgttgaca ctttaggaga tgaaaagtct gtccattcaa tattgtcaaa cccaactaaa
     7021 ccaactcgtg gcatatcaaa attgagattt ttcatggcag taaaaaccat tggtaaagcc
     7081 caacaatttg gtgcaaatac taatgtctcc tccttatctg gaactaccgc ttttagaaag
     7141 tcttctatat cactttttga atgatcatca tcttcaatca ccaaagtgtc ataaccgaaa
     7201 ccattgtcct ttaaagcatc catgaatccc gaagctctct caatacgtgt actaagcaag
     7261 ctcgtatcag cagtaatcat aataaacttt ttataccctc gatttagaca ctcctgtgtc
     7321 atatcataaa cagcatcata gttattagcc ttaacccaac tcgtcttatg ttcataaagt
     7381 tgactatcaa aaaataccat tggtttcttt ttttctttta aaatacgaga atactttcga
     7441 aaatttgatg taggctgaat aatgaaacca tcaacaccta aatttaacat attttcaata
     7501 taatcttctt cacttttggc atcataatta ctattaccca caataatttg atagcctttt
     7561 tgtttggtga tgtgctcaat tccttttaca atttgatttg aaaatgtgtt tgttatatct
     7621 ccaattaaga cacctagtag ttttgttttt ttagaattta gacttcttgc tatcgtactt
     7681 ggtctataac ctgtttcatc aatcacctct tgaatacgct gcctcgtctc atccgacatt
     7741 ttttcaaatt tttgattcaa gaaaaaagaa accgttgttt tggaagtttt tgataattga
     7801 gcgatgtcat taatggtcat ttttttagac ataacaaacc tcctttctaa ttttactgat
     7861 tttatttttt tatggttaat atagtactag tatagcataa taatagccct cttaattttt
     7921 taaacgactc attttgccat tatttttatc atatacaatg acttttcctt tgcattttat
     7981 cccttgcatc aaatagagtt tatccccaaa gataatatca tccatatgca ctccaattaa
     8041 ataatgaaag tcttgagacc taatatcaaa tgcaatgcta ttacttagtt ccttatgact
     8101 gttcgtttgc actaaaggaa cacatttcac ctgacagttc cgatcaacta tcaaggtata
     8161 atccatcacc tcatcaacaa attttatttt cttaactaat ttatgacttt ctgtcaattg
     8221 attataccgc ttagaaataa ggcaatcttc tagattaaag gtcgtaggac ttactaattt
     8281 caagtcgtta acaagcccat tttcataaat gacctgatca tctaaaatat attgatttgt
     8341 taaaacatgc tctcctggac aattcacctt atcaataact aaggtaatgg cctgcggtaa
     8401 agttaacaac catcgcttat ggtaatagat atcagaagct ctttggacat gtagttgtcc
     8461 ttcgactaaa tggtacctat cctttgaagt cagctgacaa tagtcacatt ttggataaga
     8521 attgtatgtc caagagtcct tgatcatttc taaagattgc ccatcaaggg tacatgttga
     8581 atgactagtc gaacgtttaa aatcatacct tagttgttct tctttgtaag tatatcttcc
     8641 tgcatcaatg aaaataggtt tctttttatc atagagacaa acactattat tatcactatg
     8701 agtatgagcg ctaccaaatg gaccgttctt aaaaaatata taacgcctat catcacgtaa
     8761 acacacatga ccagaatctg gaaaaagata agcagactca cctattgttg cacgcggtat
     8821 ttcttcaaaa agataaattg atggcttccc aaacaaaagt aaagtttcta aattaacatt
     8881 atcaaaagaa aatgattttg ttttagaaga tttcaacaca agtgttgcca aagttagaat
     8941 atcacgagta tcggtcacat cactgtcccc tatagctagt tgacaataat ccggaccagt
     9001 catagcaatt agataggtaa ccattttttc aatagtttct tctaatggta gataatattt
     9061 aggcgcaagg ataactagtt ccattaagga cttcaagact tctacatgat acataattga
     9121 ttgttcatac tggctcccgt catctaaaat ttgaagcttg atttgaagta ataattcttc
     9181 ttcagcaaaa ctctgaattt caggtaggtt taattcatct tcataatagt ataaacacgc
     9241 caatattgct gttgtttgta aaattcccca gttacttaga ctatcttttt cacgataata
     9301 gtccctcata taagttatct gctcccgtaa actagttaat agtttaattt ttttagtttc
     9361 tgttattaat ccaaaataat ctaaaaaaat caaaactttg agccatgaca tgcaacgtat
     9421 cccagtatca atagtacgac ttaccgcccc ttctggcttt agtgtaaatt ggcaatcaat
     9481 ccaatggtac ataaaatatt tcatttgtct aagataagac ttatcgcctt caacaatgta
     9541 gaccaccagg aatttaaaaa gataagtttg acgatttagc atatacaacc attctggatc
     9601 atctgttact ggattatccc actccaaagg atcaagacaa tatgctttag aacaaggttc
     9661 catatcccaa ttatcattaa atacaaaagt gttcctcatc atcaaattaa cagaggcttt
     9721 catttcttga taggcattag attggtatgt tttaatatac ttttgacaaa attccctatc
     9781 aaaatccttg aatttattat ataagcgatc tttcattttt ttacaaaacg tcctcgtccg
     9841 atataaaaag gactatgtca ttgatatatc ataatcctta tttatcaaaa ttttattaag
     9901 ctaaaatacc taaccagcta ccaaggatac caataataac cgtaaggata actagcttat
     9961 aagttgtcca ttttttattt ttaataaggt aaaacataag gaaagtgtag agtgctggaa
    10021 gtagagctgg tgcaatttta tcaagcatac cttggacagt aacaattttt tgcgctgtac
    10081 tagttacttt ttctggagca aaagtgtaag ggattgtaag cttaacttgt gttgcagcta
    10141 aacttgagat gaccgttaca ccaacgatat tagctgcccg tgagataact gacatttgtt
    10201 cactcaattt atcaataaaa ctggtcccaa gacgataacc atacatgcca gtaactaatt
    10261 taattcccgt taaaatagta ttcatagcca caaagaacag aatcggtccc atcaccaaac
    10321 catctgtcgc taaagaggct gcaatcgttg agaacaaggg tgctaagcaa aattgtgata
    10381 aagaatcacc gatacctgca agaggaccca taagcgccat cttaataccg cgaatttctt
    10441 cttctggccg ctcattatca agcattgcta aatgtaaact agttacaaaa ggtaagaaat
    10501 gaaggtttgt attgtaaaac tccacatttt cttctagagc tcccgctaaa gcttttttgt
    10561 catctccata atattttttg agagctggat atatgacatt agcataacca agaccttgat
    10621 agttactgta attaaagcca ttttgtaaat aaaaggcacg taaagctgtt ttagtgtaat
    10681 cactttttgc taatttttta gatccagtca tcttggttgt ctccttctgt cttagtttct
    10741 ttagtaggtg cagctggttt tctgaaaata tcgagcaaag caaatattgt tgcaattata
    10801 gctaccccga tagttggaac cccaagcaca ctgttagtag ccactgatgt taaaactccg
    10861 tctccatttg ctgttggagt tagaactggc aaaccaaaat aaacagctag aacatatcca
    10921 agtaggatat aaggaatgag ttctttttta gccattacag agagaatcat cgcaaaacca
    10981 attgcaggaa gcattttccc agctaaggtt agaccattaa gcaaaacagg gggaatgaga
    11041 gcaaataaat ctgtcaaagt ttgcgttgaa agagcaccca gtactcctaa accaaatccc
    11101 aatccagcga atgcccaaat tgtaccattt gctgcaattt taaatcctcg gaaattacct
    11161 gcttgcaaag ctgttttagc tgtttcaggc gcacctgcaa aagcagtata agttgctgtt
    11221 tgaagaaatt gaataccaac tgcaattggg gttgataatg caagagcagc ttccggtgtg
    11281 atttttcctt tagttccagc tgttgtaata gccatcaaag ttccaaaaat cccaggaccg
    11341 attgggtttg gaggaactgt ccctcccgct cccacaccaa atcccatgta agccagttca
    11401 gatattgctc caactgccaa agctgtttga atatctccta aaataacacc aacgcctaga
    11461 gagagaacga tacaacgatt tgtatagagg ccaagcaaca tgccactaaa acagaaagcc
    11521 gtccacagtc caattaagac agcctgaaga atactaatat ccatattttt ctcctttata
    11581 tgatagtgag tttgtccaac taaatataat ctaaaatatt aacttccaca gcaccatcgt
    11641 tacctgttgg agttgtttta gtattaaatg caatgtgata ttcttggttt aattttctaa
    11701 ttatctcttt atccttcatc cctaagaaga tagagcgact tacctgctct ttaccttctc
    11761 cattatgaat atttccaata ttaatctctt taattgggac gcctcctgct acaagtctat
    11821 agacatcttt caaatcttta acaattataa aaatcgtctg tgctgggttg gccttgtgaa
    11881 taatatcaat aactttttga atatcaaaaa atcgcaatgc aattgattct ggcaccaccg
    11941 tcttcattaa cgtttgttga agatgatctt tggagacatc atcatttgcc acaataacag
    12001 tattacaact taaaaatttc acccaaagtt gtccttgtcc atgaataaga cgctcatcaa
    12061 cacgtgtcat tacaatattt ggtcctgctg ccatttctga cctccttgtt ttctattatt
    12121 tattgtttat tttgttagta aaatactctt accaataaag ttcccagtct ttatagaatc
    12181 ttattaaggc ttctaagtaa taataatctc cccaaatatt accttcatct actcctttac
    12241 ctgaatgcca cgaatacaca ccgtgcaata gaagaggacg tcctgctata aattcactat
    12301 tactatactg ttcaatcaga ctacgaagca ttgtatgcat agcatatttg tatgtctctt
    12361 tatcaggatc tacttctggt aaatgtttaa gcatctcatg aatcccacac acagctgttg
    12421 ctgttgcgga tgtatctcta ggctgacccg agccatccgt aaaaataagg tcccaatagg
    12481 ataccttgtc ttcaggtaaa cgatttagaa aatagtttgt cataccttta aaaaggataa
    12541 tctgctgata atctttcatt ttccggtaac taagcggaat accgtagatg ccccatgctt
    12601 gccctcttgc ccaagatgac tcatcactat aaccctgtcg tgtgacacct tttaacggtt
    12661 ctccagtttc tgggtcgaaa taaaaagtat gaaaagcaga agaatcatca cgcaccacat
    12721 tgttagctga agcgtagaag tgattcaccg caacttgtct atacttttca tcacctgtct
    12781 gttcataagc aaaaaataag agttggatat taagtaagca atcgataatt aagcgatagt
    12841 gttccttata cccgagttct ccccaagcct gaataaatcc acctttttct tgatagcgct
    12901 ccatcaattt atctgcagct tttatagtgg cttctaaagc tttagcatca ccattgatac
    12961 gatattctgc tgtacaagat ggtgtgtaaa gaaatcctaa gtcgtggtga tctaatgcta
    13021 tacgattatt aatacgattt agaaatgaca atacattttt gtgggctatg ttttttaact
    13081 ttttatcctg attatactca taagctaacc acaagcaccc tgtccaaaaa ccatttgtcc
    13141 attctgtgtt atccataact ttataagtat tattgaatgt tgcaggcgtt ggatactctt
    13201 caccaaagta atcaatatta atataaagtt gcttcaaggc tttttcaatg gcttcctcaa
    13261 cttcaatctt agttaataat ctactgtcta aaaaacgctt tggattttca attgactcaa
    13321 ccttgaccgg ttttattttc atcattatta aatgcctccc tcaaaagttg cttctgcagt
    13381 ttgtgtagca aggcaagtag aaaaatcaac aattccttca tgagatgatg ttataacttt
    13441 atcaacaagg tcatctacat gttcaaacat cgttctagat aaaacagctt ccattaacat
    13501 tgaaaggttc aagcccgata aaacttttat ctttttatct gtatattcta ctgataaggc
    13561 ggaagaaaca ttgaaaggag tcccacctag caaatcagtc aatactaaaa tttctttgaa
    13621 atcgataacc tctctttgca aagcaactct aacatcatta gaagacatgc ctgcaataaa
    13681 gttaatacca acaacatatt cctgatgccc agcaataagt tctaaagagc ttaaaatacc
    13741 atctggaaag ttgccgtgtg ctacaataat aatttttatc atattttctc ctcttgagtt
    13801 taccggttta cttttataat catattatag cgctttcatt tttaggtgtc aacatctttt
    13861 ttatattttt tgagcagttt acttgatttt ttataatgat gtctttttta tctttaagaa
    13921 tcataactta gataaagctt tgttatatat gttgccaagt tttataggag aagaactcta
    13981 tagttgacaa aatcggttta ctttgttact attcatgtaa acaggtttac ttcctgaatt
    14041 tttttacgga ggtcatatta tgactgaaca attcttaaaa gacaactttt cactagaagg
    14101 taaagtcgcc ttaataacag gcgcttctta cggcattggt ttttcaatag caactgcttt
    14161 tgctagagct ggagcaacta ttgtcttcaa tgatattaag caggaactcg ttgacaaagg
    14221 catctccgcc tataaaaaac ttggcattaa ggcccatggc tatgtttgcg atgttacaga
    14281 tgaagatgga atcaacgaaa tggtgaacaa aatttctcaa gatgttgggg taattgatat
    14341 tctagttaac aacgcaggta ttataaaacg cacccctatg ctagagatga gcgctgcaga
    14401 ttttcgacaa gttattgata ttgatttgaa tgctcccttt atcgtatcaa aagcggtatt
    14461 acctggaatg atccaaaaag gacatggtaa aatcatcaat atttgctcca tgatgagtga
    14521 gcttggacgc gaaacagttg ctgcctatgc tgctgccaaa gggggactta aaatgttaac
    14581 caagaacatt gcttctgaat acggttctgc taacatccaa tgtaacggta tcgggcccgg
    14641 ctatattgcc acaccacaaa cagctcccct tcgtgaacgt caagatgatg gttcacgtca
    14701 tccattcgac caatttatca tcgctaaaac acctgccgct cgctggggtg aggcagaaga
    14761 tttaggtgca ccagctatct tccttgctag cgatgcatca aactttatca atggtcacat
    14821 actctatgta gatggcggta ttttagctta catcggtaaa caacctcaat aataaaggag
    14881 taatataaat gaaaattgca ttaatcaacg aaaatagcca agcgagtaaa aatactctta
    14941 tctacaaaga attgaaagcg gtttccaaaa aaaaaggatt tgatgttttc aactacggga
    15001 tgtatggcaa agaagaagaa agccagctaa cttatgtaca aaatggttta ctgactgcta
    15061 ttttacttaa tagcggtgct gcagattttg ttattacagg ctgtgggaca ggaattggag
    15121 cgatgcttgc ttgtaatagc tttcctggcg tagtctgtgg attcgctgct gattctgtcg
    15181 acgcttactt attctctcaa gtaaacggag gaaatgctct ttcccttcct tttgctaaag
    15241 gatttggttg gggagctgaa ttgaatctga ggtatttatt tgaacgtctt ttcgaagatg
    15301 aaaaaggagg gggataccct aaagagaggg ctgtacctga gcaacgcaat gcacgtatcc
    15361 ttagcgaagt aaaacagatt acttacagag atttactctc tgttttaaaa gaaattgatc
    15421 aagatttctt aaaggaaact atttctgggg agcatttcca agaatatttc ttcgctaatt
    15481 gccaaaatca aaatatagca gactacttga aatctgtctt agactaaagc gtctgtttta
    15541 gaaaggactt ttatgtctaa gattcttttt ttcggtgaac ctctcattcg catcactccc
    15601 aaagaaaatg actattttgc agatagtatc tcaactaaac tattctatgg tggctctgaa
    15661 gttaatatcg caagagctct acaaggcttt gggcaagata ctaaactcct ctcagcctta
    15721 cccaataatc ccataggtaa tagttttctt caatttttaa aagcacaggg tattgatact
    15781 cattctattc agtgggttgg agaacgtgtt gggttatatt tcttagaaaa ttcctttgct
    15841 tgcagaaaag gtgaggtggt ctacgaccgc gattatagta gtttacatga ctttaggata
    15901 aaccaaattg atttcgatca attatttgaa ggagtttctc tctttcattt tagtggtatt
    15961 acactatcat tggatgagtc tatccaagag ataacactgc ttcttttaaa agaagctaaa
    16021 aaaagagaga taacaatctc cttagatctc aattttcgca gtaaactaat ttctcctaaa
    16081 aatgccaaaa tccttttttc tcagttcgct acctttgcgg atatttgttt tggtattgaa
    16141 cctcttatgg ttgactcaca ggacactacc ttcttcaatc gcgatgaagc tacaatagag
    16201 gacatcaaag aacgaatgat ttctcttata aatcactttg actttcaagt cattttccat
    16261 actaaacgtc tacaggatga atggggacga aaccactacc aagcttacat agcaaatcgt
    16321 aaacaagagt ttgtcacttc aaaagaaatc accacagctg taaaccagag aattggaagc
    16381 ggagatgctt ttgtggcggg agcgctatat caactacttc agcactcaga cagcaagaca
    16441 gtcattgatt tcgctgtcgc tagtgcatct ttaaaatgtg ctttggaagg ggacaatatg
    16501 tttgagaccg ttaccgcagt taacaaagta ctaaatcaat cgaaagacat tattcgttag
    16561 aagggagaat tatgataaaa tcaaagattt tgttgcaatt aatgcaagga aaaatagttg
    16621 ctgtcattcg tggaaatagc caagaagaag cttttcaagc tgctcaagca tgcataaaag
    16681 gtggtatttc tgctatcgaa atcgcctata ctaatagcaa agctagccaa gtcattgaac
    16741 aactagtcac acaatatact aaccaagaac aagttgtcgt tggggcagga actgttctag
    16801 acgcagaaac tgcccgaatg gctattttag caggagcaaa gtttattgta tctcccgcat
    16861 ttaatctaca gactgctaaa ttatgtaatc gttatgctat tccttattta ccaggttgca
    16921 tgaccttatc agagataaca acagcacttg aagctggttg tgaaataatt aaaatattcc
    16981 caggaggtac tctaggaaca tctttcatct catcactaaa agcaccttta cctcaagtac
    17041 aaattatggt gactggtggg gtaaatctca caaatgccaa agactggttt ctatctggag
    17101 taacagccat tggtattggt ggagagttca ataaactagc agctttgggg gaatttgata
    17161 aaatcacaga aatggctaaa caatacaaaa tacagctaaa tctgaattaa cacaataaaa
    17221 agacaaggca cccccacacc tcaatcatga gacagaagaa aagaactttt ataaagccat
    17281 ttttctgtag taccgtttat cttaatcaga ctcattatct cgattttctt atcagaccga
    17341 gataattttt agctaatctt cctgtagaca taaccgttct acttccgaca actgatccat
    17401 gtcttcacca aagatatact acttgcccgc agattgatat aagtagtgaa gctcatcatt
    17461 ctcatatcat ttcagcaaat aatagatttg atatcatttt tatatccaaa aacttagcaa
    17521 tgacctatca ggcttacccg cttttcctat ctcaacacag gctaacttag ctcctatcaa
    17581 atacattttt taaacataat taaaaacatt tctgattata gtttattaga tttcaacaga
    17641 agtatttttc ctgcgtctca gcttttgggt gtaccgttat tttgaagttt tgatagttga
    17701 gtgatatcat taatagtcat tttttagaca ttctaaacct cctttctaat ttgaccgatt
    17761 ttattt
//
LOCUS       DATRSG010000046        17479 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRSG010000046 DATRSG010000000
VERSION     DATRSG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17479
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.046"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(18..947)
                     /locus_tag="VBD89_001425"
     CDS             complement(18..947)
                     /locus_tag="VBD89_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7135945.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            complement(931..1623)
                     /gene="ftsE"
                     /locus_tag="VBD89_001426"
     CDS             complement(931..1623)
                     /gene="ftsE"
                     /locus_tag="VBD89_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO7135946.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            complement(1642..2740)
                     /gene="prfB"
                     /locus_tag="VBD89_001427"
     CDS             complement(join(1642..2667,2669..2740))
                     /gene="prfB"
                     /locus_tag="VBD89_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO7135947.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            complement(2809..3933)
                     /gene="queG"
                     /locus_tag="VBD89_001428"
     CDS             complement(2809..3933)
                     /gene="queG"
                     /locus_tag="VBD89_001428"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO7135948.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            complement(4023..5954)
                     /locus_tag="VBD89_001429"
     CDS             complement(4023..5954)
                     /locus_tag="VBD89_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO7135949.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSVS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFAPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKGVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIVWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            6106..6966
                     /locus_tag="VBD89_001430"
                     /pseudo
     CDS             6106..6966
                     /locus_tag="VBD89_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(7011..9695)
                     /locus_tag="VBD89_001431"
     CDS             complement(7011..9695)
                     /locus_tag="VBD89_001431"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO7135950.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(10039..10437)
                     /locus_tag="VBD89_001432"
     CDS             complement(10039..10437)
                     /locus_tag="VBD89_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO7135951.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGIQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGACFANYELKIQWY"
     gene            10501..11817
                     /locus_tag="VBD89_001433"
     CDS             10501..11817
                     /locus_tag="VBD89_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7135952.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            11889..12701
                     /gene="yidA"
                     /locus_tag="VBD89_001434"
     CDS             11889..12701
                     /gene="yidA"
                     /locus_tag="VBD89_001434"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO7135953.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKDVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            12686..13921
                     /locus_tag="VBD89_001435"
     CDS             12686..13921
                     /locus_tag="VBD89_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7135954.1"
                     /translation="MGNKLMYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQ
                     NERLGFYKDDQLVAVASILIKSLPLGFTMLYIPRGPIIDYSNKELVGFVLKTLKNFGR
                     KKRAVFAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQ
                     PRFQANIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKG
                     VALRNENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQRDLSETPSHQKSRL
                     TRLNQQEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYP
                     QYLLNARVFEDAFKNGIVSANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPL
                     LYKLANLAYTIRKKQRHSH"
     gene            13934..15145
                     /locus_tag="VBD89_001436"
     CDS             13934..15145
                     /locus_tag="VBD89_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7135955.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWLKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            15164..16399
                     /locus_tag="VBD89_001437"
     CDS             15164..16399
                     /locus_tag="VBD89_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7135956.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDGLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            16587..>17479
                     /locus_tag="VBD89_001438"
     CDS             16587..>17479
                     /locus_tag="VBD89_001438"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO7135957.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLKAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIFHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGY"
BASE COUNT         5744 a         3401 c         2805 g         5529 t
ORIGIN      
        1 gatttcatct ctttttatta aatcttaagg taacgtctca ttgataggac agatccaagt
       61 gagccaataa taataccaat aatcaccata ccaccaataa ttgctggaac cataatatcc
      121 ataggataca atgaaacatg gcttgtctca aattttggat taaacttgtt aaacacaaat
      181 tgataaccaa aatagaaaat cagcgatgga acgatagccc ctaaaatccc tacccaagca
      241 ccttcaaaaa agaacggtcc tcgaatataa gaatttttag cgccaactaa tctcatgatt
      301 tcaatatctg tacggcgtga cataatcgac atacgaatag tatttgaaat caaaaagata
      361 gcaaggacaa ttaataaggc aacaccacca aatccccata tacggatgtt atctgtgaga
      421 ttagatagtt ttgttgaatc aataggttct ttagtataat caacaccttc aatgcgtcca
      481 attgcttcac tgacactctt agaaactttt gcagaacttg tcccaactac gtaaacatct
      541 aacaaagcat ctttgtaggt atcatcaata acatctgaac catactcttt ttgaacttct
      601 tttaattgtt cagctttact tgaataagtg actttctcta caccagaaat acgtttaatc
      661 ttgtcataaa ctttatgata atcaggatta tcttttagct ttccgtcctt atctttgact
      721 tgtttttgag catccgttga gtcaacattt aaaaaggcac tgatagtaaa attacctgaa
      781 acatcagttg ttaacttttc aacattcagt aaaactgatg aaaacaagcc tacaagaagt
      841 aatgttatgg taactgatgt tacagaagca aaagtcatcc aaaagttacg ttttaagttt
      901 ttaagcgatt cccagaaatg tctaaagaaa ttaatcatga taaccatatt ctcccttctc
      961 ttcatctcgg ataacgctac ctgcttcaat ctcaatgaca cgatgacgaa gtgtgttaac
     1021 aatttgacta ttgtgtgttg ccatcaaaac agtcgttcct tgaaggttaa tacgttccaa
     1081 tagatgcatg atttcccaag cgatttctgg atccaaatta ccagtaggtt catctgcaat
     1141 taataatttt ggattattaa caatcgcacg tgcaattgcc acacgttgtt gttctccacc
     1201 agataactgt gttggaaaag aacgcatctt gtgttttaaa cctactaatt ctaaaacttc
     1261 tggcacgcgt tttttaatat gacggcgctt agctccaata acttgcatcg caaacgcaac
     1321 attctcataa actgtttttg ttggtagtaa tttataatct tggaagacaa ctccaatact
     1381 acgacgtaaa ataggaattt gacgacgttt gagtttattg agattgaatt cccctacctt
     1441 taatctacct gaacttaatt tttcttcacg gtataggagg cgaatcaagc tagatttacc
     1501 agcacctgac ggaccaacta aatacacaaa ctcaccttgc tgaattgata aatttaagtt
     1561 tctcaaggca gttgttgaac ggcgatactt tttagtaaca ccagacattt ctattagtgc
     1621 catgtttctc cttcattatt tttagtcttc aatgcgccac ttaaggtagg catcaataaa
     1681 gccattgatt tctccatcca taaccttatc tacctgagca agctcaaagt tagtacgatg
     1741 atccttcacc atcgtataag gggtgaaaac ataagaacga atctgacttc cccaagtgat
     1801 ttctttttta tcacccttga gagcatctac ttcttgtgct ttcttttctt gttctaactg
     1861 atatagctta gcctgcaaca tcttcatagc gcgatcgcgg ttcccatatt gagtacggtc
     1921 aacggtagac gataccacaa ttccagttgg aatatgcgtc aaacgaacac ctgttgatac
     1981 tttattgacg ttctgaccac cagctcctcc ggaacggaaa gtatccattt taatgtcatc
     2041 atcacgtact tctacttcaa tcgtatcatc taactctggc atcacttcaa ctgaagcaaa
     2101 tgaggtatga cgacgtttag ccgaatcaaa aggtgatata cgaacgaggc gatgaactcc
     2161 catttctgat ttcaacaaac cataagcatt gggaccttca aaagagaggg taaccgattt
     2221 aataccagct tcatcccctg cttgataatc caatacttct actttgaaac cattagcatt
     2281 gccaaaacgg gtatacattc gtaagagtaa atctccccaa tcttgcgctt cagttcctcc
     2341 tgaaccagga tggatttcta agatagcatt attgtggtca taaggctcag ataagagaag
     2401 cgtcatttca taagcaccca ttatcttatc tagctgcatt agattttctt ctaattcttc
     2461 tttcaaagaa tcatcttcgt caagcatctc aagtaagagt tctgtttcgt cagataattc
     2521 ttgcatatta tggaaagtat catacttacc ttttaactca ttcagttctt gagatgtctt
     2581 ttgcgctgca atattgtcat tccaaaagtc tggctcagtc atttgatttt caagaagagc
     2641 aatctcctct tccaaacgat ctaagtcaaa gagacctcct gaagctagtc aacttctctt
     2701 tattttctac tattttttgg cgaatttcag ccacttccat gagatttctc caatctttgt
     2761 taatctgttt tattttatca taaggttctt aaaaatacca taaaaggctt aaaattgcca
     2821 tttatgacgg attaattcca actcttttcg gctatcttca tcttttaagg ttaaattact
     2881 cataaactcc agtatctcat cgtttggttt tttgacaatt tctcccaatg cccatatagc
     2941 tgttgcagta tgaatagggt tattattttt atcaataatc tctattaatt taaccactgc
     3001 ggttttatca tgagcatttg ctagcgcaat aatggcatta cgctgtaaaa tatttttacc
     3061 tcgccaactt ccagcaatca taccaaattt ttctttaaat tgtccatttg ataaactcaa
     3121 aaatggtatt aattctggtt gagccaactc aggatcaatc tctgtcgcca atggattatt
     3181 tatacctttg ttataaggac agcaaatttg gcaaatatcg caaccataga taactgtttt
     3241 aatctttttt cgaaactcta tgtccatcat tcccttatct tgggtttgaa atgatagaca
     3301 acgcttggca ttcattgagc catcaccaat taaacaagaa gttggacagg catctagaca
     3361 acgacgacag tcaccacaat cataatcgac aggcttgtct ggctcgatct ctaaattggt
     3421 aattaattct cccaaaaaca tataagagcc atattcttta gaaataacta gaccattttt
     3481 tcctataaaa ccaatacctg ctctttgggc aactgcagta tcaactaaag ctccagtatc
     3541 gaccattgct ttttgttgca aaggaaaatc tcgacagagc tcttcaatgc cttttgatag
     3601 tctgtctaac ttttcgccga ccacataatg ataatcaagc ccccacgaat taggtgtaat
     3661 ttttcctcgt ttataaggtg ttttttgagg ttgctgagga agtttgtgag gataagcaac
     3721 gccaattgaa ataatcgttt tagccgattc taaaagcctt tcagggtaga tacgatcctc
     3781 tatgacttta tgttcaaatc ctgaattgcg cccctcttcc acagaagcac gcaatgactt
     3841 ttccaaataa tcaaagttat ctgccgtcgt aaaaccaatt ttagagatac caatttcttt
     3901 tgctaatttt tgaatttctg cctttatgtc cataatatta ttttaagtca atttaataaa
     3961 agagacaagg taagaaactg aaaaattaaa tcctacatat caattagtaa attacttaaa
     4021 cactagtcat catattcctg aaaatgacga taaaggtgat ctaagtcttt aatctctttg
     4081 gttaactttt gtccaatagt tgtatctttt acaagaatcc tatccttggc ttcttctact
     4141 aatcgcttcg tatcaatgat atagttggtt ccacttaaaa cttcctcaat ggatgtaaaa
     4201 ggaaggtgtg aaattaattg tatcccatag ctattataaa tcaaagtgta gccagctaaa
     4261 cctgtatttt tctgataacc tttggcaaag ccaccatcaa ttactaacat cttaccattt
     4321 gcttttatcg gttgctcacc atttttttct ttgactggcg tatgaccatt gacaatatgt
     4381 ccaaacttag gcaaatcaaa ctcttgtaaa agggcagtgc aaatatcatc tttatctctc
     4441 agtttaaagt agggattttt tctttcttga tgagttactt tgtcggaaac ataatagcgc
     4501 tcaaaagtag tcatggcatt cttaccaaaa agagaagaat tttcaccaca ccaaagatac
     4561 caaacgatat ctgtcgccaa atcatcatgt ttctctggtc tagcatatgc tagacgtatt
     4621 tgactttcaa ataaatccag taaatcccta ccaccataag tttttcctgc aattttaaag
     4681 cttttaaaat caccattact atgcattggt atgcaaccat ggaataaaag attatcatta
     4741 taggttaaat acatacttcc tttctcaaat aggaaatcaa tatgtttctt taaactttca
     4801 gaagtctgga aggctatcat caattgttgt aaaagctctt cttcctcact tgttaatcga
     4861 ctgggatttt tcgaatcaat gcatctacta ttaaaatctt taagttgatg cacaactccc
     4921 tttatagata tacttaaatc tttataattt acctggttaa taagttgtcg attatgcatt
     4981 tgaaattctg gacgcctgtc aatcaattgg gcttccaatt taaactgtaa aattgctgtt
     5041 gcttgttgta tcatatttaa caaatctaat tcatctggat gagtcatttc ctctccatct
     5101 aatatggggg cgaaagaagg tagaggttca tagtaacggc gtgagtaatc aaccaagcga
     5161 cgcaaattga taccatatcg gtcttcaatt aaagtaatat tattataacg tgcagcaata
     5221 cggataacat ttaccataca aaggtaggaa ccagatgctg ctcccatcca agtgacatca
     5281 tggttccccc attggatatc caaatttgga aaagccatta acctattcaa gatacgatca
     5341 ggatacctac cacgatcgta aatatcccct acaacatgta aatggtcaat gattaaaact
     5401 tgaattgttt gggataatac tataattaaa tcttctactt ggtctaattc aaataatttt
     5461 tcaataatag tatcaaaata gcttttcttc tctgggttac ggtcaatctc agctaaaagt
     5521 tcttcgataa tgtaagcaaa cttacctggc aattgcttac gtactttaga ttttgtatat
     5581 ttactagaaa aatattttaa aacttgtatt tgtaacggaa tcatttccca taacttcgtt
     5641 ttataagcac ttactgacaa gttcatttta tcgaaggcta atttttcctt tgggtaataa
     5701 agcagtatac aaaaatcatc taaatcaaca gcaactattt tctgccaatc aaagcagtct
     5761 aataatttag ctctaataga ccctgcacct gtccgtaaga gataatcaac agcatcgtat
     5821 tccccgtgta aatcactaat aaaatattcc gtccctttgg gtaattgaca aatagcttct
     5881 aaattgatca tttctgttac gatatcttct tttcgaggaa atttttcttt caagagctta
     5941 taaaaattag acatgagacc tccttaaggc accttaaata taaagcttct aaacacaatt
     6001 atgaaagcgc attctacaat gacatatcta ttatagaatg actttaaaac tatttcaaaa
     6061 atttactgtt tatctctttg aactacaata tggtacaata cccttatgac caaacttgct
     6121 atcatgagtg atttacatat tgacctcaat catttttcta cctttgagac gaaaacgtta
     6181 attaatttgc ttaagaaaga aaaaattgat cacctccata tcgctgggga tatatctaat
     6241 cattttacta aagatacact tccctttatc aataacttaa aaaagcatat taaacttagc
     6301 tataatttag gaaaccatga tatgcttgat ttaacagaaa cagaaatcca acgccttgac
     6361 tttcaaacct atcgatttga caaaaaaatg ctactagctt ttcatggcta gtatgattac
     6421 agcttttcaa ataatagaga tatcaaggat gttgaaaaat taaaaaagac tttttggttt
     6481 gatagacgtt taaaacgtcc aaataatgat gtaactatcc aagcatcaat tttaaaacgc
     6541 ttagacgaaa tattagcaaa agtcgattct tcaaatatca ttattgctat gcactttgtt
     6601 ccccataaac agttcacgat gactcatcct agattctctc cttttaatgc ttttttgggg
     6661 agtcaggctt atcatgattt attccaaaaa tatcatatca aagatgttgt ttttggtcat
     6721 gcacaccgct catttggcga cgttaaaatt ggagagacta cttatcattc tagaccactt
     6781 ggatatatta gagaatggaa tttaactatt gactttgtca atcagaatcc caatcataac
     6841 cctaatttaa cctggaatct tagcaaaagg cataatgccg ttaagcattt ggatagtttt
     6901 gaaaactatc caaaaaaata ttttgaagac gaattacgca actctatgac aatatttgat
     6961 tgctaaaaaa ctcacttaga tattctaagt gagttttttt atcagctaaa ctaaatagct
     7021 ttttcgtctt gtcctaattt acgttggata aatttaacaa tttccgttaa cactaccatc
     7081 aataaacttc cgataactac agttaaccat tgtgttgaac ttaaatgagt tacatggaaa
     7141 agtttattaa atccaggaac aacaatagtc accatcaaaa gaatgaaagc tactggaata
     7201 gaccagttaa aggttctatt tttaaaagct ccaactgtaa agattgattg gtaaactgat
     7261 ttaacgttaa atgcatgtac caattggatt aaaccaagcg ttgcaaaggc catcgtaagg
     7321 gcatctgcgt gaatcatgcg ataaccagca tgttctggat acatcaatgc ccaaccatag
     7381 acaccaagta ctaagatagt ttgtaaaata ccttgataga taatagctcc cataacacca
     7441 ccatcaaaga agtttgactg gcgaccacga ggtttatgtg tcataactcc tggttcagct
     7501 ggctcaactc caagagcgat agctggaaga gtatctgtta ccaagttaat ccaaagaagg
     7561 tgaaccggtg caagtacatc ccatcctaat aaagttgcaa agaaaattgt aaatacctct
     7621 gccatgttgg ctgaaagaag atattgaata gatttttgga tatttgagaa gactttacgt
     7681 ccttcctcaa cagccacaat aatagttgca aaattatcat cagcaagaac catatcagaa
     7741 gcacctttgg aaacttctgt accagttatt cccataccaa taccaatatc tgctgttttt
     7801 agcgatggag catcattaac accatcacca gtcatggcaa cgactttgcc atcgttttgc
     7861 caagctttca cgatacgcac tttatgttca ggagaaacac gtgcatagac agaatattgt
     7921 ttaaatactt tttgaaactc ttcgtcactt aattcattaa gttcagcacc cgtaaagaca
     7981 tgatcttctg tgtcatttgc atcaataata cctagacgct ttgcaatagc ttctgcagta
     8041 tcttgatggt ccccggtaat cataatcgga cgaattcctg cttctttagc aactcgaaca
     8101 gcttctgccg cttctggacg ctcagggtca atcattccca ctaaacctga gaatactaaa
     8161 tctgactcaa caatatcagt ctctaaagtt ggaatttgtg tttcatattt ataagccatc
     8221 atcaagacac gaagagcttg ttttgcgagt tctttattgg tatttaggat agcctcttta
     8281 tcttcagccg tgatatcacg aaccaagcca ttatcttcaa ttttagtaac tcgttttaat
     8341 aattgatcag gagcaccctt gactgcgata aagtagcgac catctgattc tttatgaatc
     8401 gtagacatta acttacgatc tgaatcaaat ggtaactcag cgacacgtgg ttcatttttc
     8461 aaaacttcac ggacatcaaa attcttatct aaaccaaatt gtaccagtgc agtctctgtt
     8521 gggtcaccga ttaattttcc tgaaggatca atcttagtat cgttggagaa attcataata
     8581 cgtaaagtgt tattatcaac tgaaatttct tccgaagatg attggagaac gccattagta
     8641 tacacttttt cgacagtcat ttgattcatt gtcagagtac cagtcttatc cgatgcaatg
     8701 atttctgtag aacctaaagt ttcaactgca gggagtttac gaacgataga attacgttta
     8761 gcaagagttt ttgttcccat tgataagaca atcgtaacaa ttgcaggaag cccttctgga
     8821 atagctgcaa ctgctaaagc aactgaagtc atcaatcctt caatccaacc ttctttacgg
     8881 acaaatatcc ccactaagaa agtaataacc gcaataatca cgattaagta agtcaataat
     8941 ttagaaagtt gaactaaact ttgtttcaat ggtgtttcag attcatcagc atttgccaac
     9001 atatcagcaa ttttaccaac ttcagtatac atccctgtgt tagtaacaac accatatcct
     9061 cgaccatacg tgacatttga attttgatac gccatattca cacgatcacc aatacctgca
     9121 tcctctgcta ctacttgaga tatgtctttc tcaacaggta ctgactcacc agttagagct
     9181 gcttcttcaa tttttaaaga cgcggcttct aaaagtcgta aatctgcggg aacaacatct
     9241 cctgcttcta acataacgag gtcaccagga actagttctt tagaatccac ctcaatagta
     9301 tgaccatcac gacgaacacg cgcaattggg cttgacatat ctttgagggc ttcaatagct
     9361 gcttcggctt gcccctcttg ataaacacca aaagctgcat ttaagataac aactgccaag
     9421 ataatcaatg catccgttaa gccatgcata ccttccgtaa taacagagag agctgcagca
     9481 accagtaaga taataatcat taaatctttg aattggtcaa aaaacttagc tattagacta
     9541 cgttttttcc cttcttcaag ctcattgcga ccatacatct ctaagcgatt tttcgcttct
     9601 gttgttgata atccttcacg agaagattct aaagacgtta gaacctcttc ttgaccttgt
     9661 gtgtaaaaca aggatttttt ctgttctttt aacaaaacct ttcctccttt ggtctctcca
     9721 caaacaaaaa gagacctgtt tttatgaaaa caagtctcgc tgtttaagac aacgccggaa
     9781 atttctttcg taatgacgac attgccacgg taaaccgtct gctactccct tgatatgtta
     9841 atcattgtaa cagaaaaaaa tccatttttc aattaattat ccttgctatc actaataatc
     9901 tcaagggaaa aaacgaagtt catctagtcg aaataaacca tactgcagtt tatcaaataa
     9961 atggctctca taaagagatc ccagcgtctt cagtagacga aattcataat gactactctt
    10021 ttgttaatgg taattatctc aataccattg aatctttaat tcataatttg caaaacaggc
    10081 tcctgtctcc gtttgcttaa ggacataatt gatgtgcaac ctttgctgtg acaaatcaaa
    10141 ctgatagtga cttgtctctg taatcaaatg ttgaatacca agtggagtgg ggatattaac
    10201 caaagcaggc gtttcacgat gaaaacgcat tacagactta ggatttgaaa acctcgtcat
    10261 aagcaattcc tcatgatttg ccttaataac tactctctcg ccctcagcat taatataatt
    10321 caaataaacg taacttcctt tttcaataaa ttgacaatcg tgaatctcat gaatcatttc
    10381 ggtttcgttg tcaatttgaa tgttattgtt aatttctagt ttcatgactt ctctcacttc
    10441 ttttatcctt ctattctacc aatttctgac tcaagtttca atttttgcta taatatatat
    10501 atgaatgaaa aagtatttcg tgaccccgtt cacacttata tccatgtaaa taatcaagtc
    10561 atatatgact tgattaacac taaagaattt caacgtttac gacgcattaa acaaacctca
    10621 acgacttctt ttaccttcca tggtgcagag catagtcgtt tttcacattg tctcggagtt
    10681 tatgaactgg ctcgtaaagt tacagaaatc tttgatgaac attattctga tctttggaat
    10741 aaaaatgagt ccctgctaac gatggctgcc gccctactac atgatatcgg acatggcgca
    10801 tattctcata catttgagcg tctcttcaat actgatcatg aagcttacac tcaggaaatc
    10861 atcaccaatc ctaccacaga gattaatgct attttacgaa aggtggctcc tgattttcct
    10921 gataaagtag ctagtgttat taatcatagc taccccaata aacaagttgt tcaattaatt
    10981 tctagtcaaa ttgattgtga tcgaatggac tatcttctca gagattctta ctacactgct
    11041 gctagctatg gacaatttga tttaacacga attttacgcg ttattagacc aaccgattct
    11101 ggaattgctt ttgctcgtaa cgggatgcac gctgtggaag attatattgt tagtcgcttc
    11161 caaatgtata tgcaagttta tttccatcca gccagtcgtg ctatggaatt actcttacaa
    11221 aatttactaa aacgtgctcg cttcttattt gatacccatc gcgatttttt tgaacaaact
    11281 tctccaaatc ttattccttt cttcacggat caatatgatt tacaagatta tttagcccta
    11341 gatgatgggg taatgaatac ttacttccaa tcttggatgc aagctgatga taacattttg
    11401 gcagatttag ctaatcgttt tatcaatcga aaagtcttta aatcaattac ctttgaagag
    11461 gctgataaag aaaatctagt taaaatgaaa gaactagtct cacaggttgg ttttgatccc
    11521 gattattaca ctggtgtcca tgctaatttt gacttacctt acgatgttta tcggcctgaa
    11581 cattcaaatc cacgaacaga aattcaaatt atccaaaaga atggacaact tgctgaatta
    11641 tcaagcttat caccgattgt aaaagcatta actggatcta attatggtga tcaacgtttt
    11701 tattttccaa aagaaatgtt gacattagat agcctatttt caagtacaaa agaagaattt
    11761 caatcttata tcacaaatga gcatttgaca ctcacgaaag ataatagcca ttcatgacaa
    11821 accctttatt ttttagtaag atataaggtg agtattttag gatattgaaa taaagagtgg
    11881 gagataaaat gacttcaatt aaacttgttg ccgtagatat tgacggcacc ctattaaata
    11941 gtaagcgtga aattacacct gaagtggcta aagctgttca agaagcaaaa tcaaaagacg
    12001 ttaaaattgt tattgctaca ggacgtccaa tcatcggagt ccaagatctt ttagaagaat
    12061 tgaaactcaa tgaagaggga gattatgtta tcacctttaa cggcggtcta gttcaagaca
    12121 cagccacagg agatgacatc atcaaagaaa ccttaaccta tgaagactat ctggatttcg
    12181 aactgttagc gcgtaaatta ggcgtccaca tgcacgccat tacaaaagaa ggaatttaca
    12241 cagcgaatcg tgatattggt aaatatacaa tacacgaagc aactttagtt aatatgccgc
    12301 tcttctatcg tactcctgaa gaaatgggag acaaagaaat cattaaacta atgatgattg
    12361 atcaacctga catcttagat gcagcaattg ctaaaattcc aaaaaaagtt ctcgataact
    12421 acaccattgt aaaatcaact cctttttatt tagaaatttt acccaaaaac gttaataaag
    12481 gcactgcact tctccacctt gcagagaaaa tgggattaac tgtagatcaa acaatggcta
    12541 ttggtgatga agaaaatgac cgtgctatgc ttgaagttgt tggaaatcct gttgttatgc
    12601 aaaacggcaa tcctgaacta aaaaaaatag caaaatatat caccaaatca aacgaggaaa
    12661 gtggcgtagc ctacgcacta cgtgaatggg taataaactg atgtatcatg ttaccgttgg
    12721 aatatccgaa aaagaatatg atgcctttgc tattgcatcg tctcaaacca atctcctcca
    12781 ctcttcaaag tgggcacaag taaaatcaaa ttggcaaaat gaacgtttag gcttttataa
    12841 agatgaccaa ttagttgctg tagctagcat cttaattaaa tcattacctc ttggttttac
    12901 aatgctttat attccacgtg gacctattat agactacagc aataaagaat tagttggctt
    12961 tgtgttaaaa acactaaaaa actttggacg aaagaaacgt gctgtttttg caaaattcga
    13021 tccagcactt cttctacgtc aatatcacct taaagaagag aatgtcgccg aagagataga
    13081 tgaaagtaga caggcaattg ataatcttaa gtcagcaggt gcccaatgga taggtccaac
    13141 gaaagccatt tccgaaacca tccaaccacg ttttcaagcg aacatctata ccaaagcaaa
    13201 tatagaagaa aactttccaa aacacactaa acgtctgata aaagatgcaa agcatcgcgg
    13261 tgttcaaatt tatcgtgcca acattgatga tttacctaaa ttcgccacgg ttgtagcatt
    13321 aaccgagaat cgtaaaggtg tagctttacg taatgaaaac tatttccatc aactaatgac
    13381 aatttatgga gaagatgcct atctctattt ggctaaagtc aatcttccta aaagattagc
    13441 gcaatttaaa gaacaacttc ttcaaatcca aagagattta tcagaaactc catcccatca
    13501 aaaaagtcgc ctgacaaggc taaatcaaca agaagcttct gttaaacaat atatccttga
    13561 atttcaggag tttagcaaga aatatccaga tgagcctgtc attgcaggaa ttttgtccat
    13621 tcgttttggc aatgtcctgg agatgttgta tgctggtatg gatgatagct tccgtaaatt
    13681 ttatccacag tatctcttga atgctagggt gttcgaagat gctttcaaaa acggtattgt
    13741 atctgctaat ctaggcggtg tcgagggaag tcttaacgat ggtttaacaa aatttaaaag
    13801 taacttcaat ccaatgtttg aagaatatat tggcgagttt aatctagcaa tcaatccatt
    13861 attatataaa ttggccaacc tagcctatac tattcgaaaa aaacaaagac actctcacta
    13921 agaaaggaaa ctcatgtata gagaaattac cgctgtcgaa cacgatcgct ttgtgagcga
    13981 atccaaccaa acaaacctac ttcaatcttc taattggctc aaagtaaaag acaactgggg
    14041 tagtcaatta cttggctttt ttgacggtga aacccaaatt gccagcgcta gtattctcat
    14101 caaatcactt cctcttggct tctccatgct gtatattccg cgtggaccaa tcatggatta
    14161 ctccaatcta gatattgtaa ctaaggtcct taaggacctt aaagcttttg gcaaaaaaca
    14221 aagagctctc tttatcaagt gtgatcctct catctattta aaaatggtca atgccaaaga
    14281 ttttgaaaat tcgcccgatg aaaaagaagg gttaatcgcc attgatcatt tacagcgtgc
    14341 aggtgctgat tggactggtc ggacaacaga tttagctcat actatccaac cacgttttca
    14401 agcaaaccta tatgctaatc aatttggact tgataaaatg tctaaaaaaa ctcgtcaagc
    14461 tattcgaact tccaaaaata agggagtaga tatacaattt ggtagccatg aactacttga
    14521 agattttgca gagttaatga aaaaaacaga agaccgtaaa gggataaatc ttagaggaat
    14581 tgactactac caaaaactcc ttgatacgta tcctaataac tcatacatca caatggcttc
    14641 gctagatgtt gcaaaacgat tagaaaaaat agaaaaagaa tgccagatag ctcaatctga
    14701 aagaataaaa tcacttgaac ttaatcgtga gaaaaaagtt aaacaacacc aagatacgat
    14761 tgatcgatta aataaagaaa ttgattttct caaagaagct caaaaagcct atgaccgaga
    14821 cattgttcca ttggctgcaa ccctcactct agaatttggt aatacatcag aaaatattta
    14881 tgctggtatg gacgattact tcaaatctta ttctgctcct atttacactt ggtttgaaac
    14941 agctcaaagg gcttttgaac gtggaaatat ttggcaaaat atggggggga ttgagaatga
    15001 cttatctggt ggtttatatc attttaagtc taagttcgaa ccaatcattg aggaatttat
    15061 tggagaattt aacattccag tgaacagatt gctttataaa gcctctaatt atgtctatgc
    15121 cttacgcaaa aaacgtaata gctagttagg aaaggatact tttatggcgc ttaaagaatt
    15181 aacagctaaa gagttcgaaa gttattcagg aaattacgat ttacaatcgt ttatgcaaac
    15241 ccctgagatg gcaaagcttc taaaaaaacg tgggtatgac attacctaca tgggctacca
    15301 aatcgatggg aaaatggaaa tcatcagtat tgtttatacc atcccaatga ctggtggact
    15361 tcatatggaa gtcaattctg gtccagccca ttccaattct aaatatctga aacattttta
    15421 taaagaacta cagaactatg ccaaatctca gggcgcttta gaattattaa ttaaacctta
    15481 tgatacctac caagagttta ctggtgaagg tgaacctaaa ggtgcaccga acacctatct
    15541 tattgatggc ctgactagta taggttatca ccacgatggg cttcatattg gttatcctgg
    15601 cggtgaacct gattggcatt atgttaaaaa tctagaaggt atcactcctc aaaatcttct
    15661 taaatctttc tctaaaaaag gacgcccctt ggttaagaaa gccatgtctt ttggtataaa
    15721 aatacgtgtc ctcaagcggg aagaactaca tatctttaag gatattactt cttctacttc
    15781 tgatcgccgg gactatatgg ataaatctct agattattat caagattttt atgatagttt
    15841 cggagataag gcggaatttg tcattgcaac tctaaacttt agggaatatg atcataacct
    15901 acaattaaat gcaaagaaat tggaagaaca gattacagtc cttgataatc gccatcaaaa
    15961 taatacagat tctgccaaat accatcgtca acgtactgag cttgtcaatc aactagctag
    16021 tttagataaa cgtcgcaaag aagtcgaacc ttttattcaa aaatttggaa atcaagatgt
    16081 tgttttagct ggtagcctct ttatttatag ccctaaagaa actgtctatc ttttctctgg
    16141 ctcatacaca gaatttaata aattttatgc gcctgcagtt cttcaagaat atgttatgca
    16201 agaggctcta aagcgtcaat caactttcta taatttttta ggtattcaag gaaattttga
    16261 tggttcagat ggcgtacttc gctttaaaca gaattttaat ggttacattg ttcgtaaaat
    16321 gggtactttc cgctactatc caaatccatt aaaatataag agtattcaat tactaaagaa
    16381 aatcttacgc cgaacataaa aaataagctt acataagctt atttttttat gcattataga
    16441 aagcaacatt tatttcttta ctattattgt ttacacaaat aataacgttc gttatttagc
    16501 gaaattttat taccaatatc attattttct tgccgctctg ttaatcaagt gataaaatag
    16561 taaaaattta tcttcaggag gatattatgg tatcgctaaa aacggaaatt gctggtttta
    16621 gttttgacaa ttgtttgatg aatgcagcgg gtatttattg tatgactaag gaagagttat
    16681 tagctattga aaattctgaa gctggttcct ttgttacaaa aacaggtacc cttaaagcta
    16741 gagagggaaa ccctcaacca cgttatgcag atactgactg gggatcaatt aattctatgg
    16801 gactccctaa taaaggtatt gactattatc ttgatttcgt gactgaatta caagaccaag
    16861 acaatagcaa aaatcacgtt ttatcattag taggtttatc accagaagag acacatatta
    16921 ttcttaaaaa ggttgaaaac agtagttaca atggcctgat agagttaaat ttatcgtgcc
    16981 caaatgttcc tggaaaacct caaattgctt atgattttga aatgactgac ctcatccttt
    17041 cagaaatctt tagctattat caaaagccat tgggcatcaa attaccacct tactttgata
    17101 tctttcactt tgatcaagca gctacaattt ttaacaaata tcctttagct tttattaact
    17161 gtgttaattc tatcggaaat ggacttgtta ttgatgatga aactgtcgtt ataaagccta
    17221 aaaatggctt tggaggtatc ggcggtgatt ttatcaagcc taccgcctta gctaacgtcc
    17281 atgctttcta caaacgttta aatccttcaa ttaaaattat cggaacaggt ggtgttaaaa
    17341 acggccgtga tgcctttgaa cacattctat gtggagctag tatggtccaa atcggaaccg
    17401 ctttacaaaa agaaggacca gaaatttttc agcgtgtaag cagagagtta aaagaaataa
    17461 tggctgataa aggctatca
//
LOCUS       DATRSG010000047        17300 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRSG010000047 DATRSG010000000
VERSION     DATRSG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17300
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.047"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            150..1394
                     /locus_tag="VBD89_001439"
     CDS             150..1394
                     /locus_tag="VBD89_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7135958.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            1396..2679
                     /locus_tag="VBD89_001440"
     CDS             1396..2679
                     /locus_tag="VBD89_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7135959.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            2802..3344
                     /gene="pgsA"
                     /locus_tag="VBD89_001441"
     CDS             2802..3344
                     /gene="pgsA"
                     /locus_tag="VBD89_001441"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO7135960.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            3344..4183
                     /locus_tag="VBD89_001442"
     CDS             3344..4183
                     /locus_tag="VBD89_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7135961.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            4159..5001
                     /locus_tag="VBD89_001443"
     CDS             4159..5001
                     /locus_tag="VBD89_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7135962.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDVSYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            4994..5788
                     /locus_tag="VBD89_001444"
     CDS             4994..5788
                     /locus_tag="VBD89_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7135963.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            6004..6543
                     /locus_tag="VBD89_001445"
                     /pseudo
     CDS             6004..6543
                     /locus_tag="VBD89_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            6649..7353
                     /locus_tag="VBD89_001446"
     CDS             6649..7353
                     /locus_tag="VBD89_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO7135964.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQTYAVTETTYKPAQHQTSGQVLSNGNTAGAVGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(7488..8360)
                     /gene="sdaAA"
                     /locus_tag="VBD89_001447"
     CDS             complement(7488..8360)
                     /gene="sdaAA"
                     /locus_tag="VBD89_001447"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO7135965.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(8375..9043)
                     /gene="sdaAB"
                     /locus_tag="VBD89_001448"
     CDS             complement(8375..9043)
                     /gene="sdaAB"
                     /locus_tag="VBD89_001448"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO7135966.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            9289..10410
                     /gene="mnmA"
                     /locus_tag="VBD89_001449"
     CDS             9289..10410
                     /gene="mnmA"
                     /locus_tag="VBD89_001449"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO7135967.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            10442..11053
                     /locus_tag="VBD89_001450"
     CDS             10442..11053
                     /locus_tag="VBD89_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO7135968.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            11223..13124
                     /gene="mnmG"
                     /locus_tag="VBD89_001451"
     CDS             11223..13124
                     /gene="mnmG"
                     /locus_tag="VBD89_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO7135969.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            13191..15173
                     /locus_tag="VBD89_001452"
     CDS             13191..15173
                     /locus_tag="VBD89_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO7135970.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDVERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            15179..15631
                     /gene="rplI"
                     /locus_tag="VBD89_001453"
     CDS             15179..15631
                     /gene="rplI"
                     /locus_tag="VBD89_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO7135971.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            15674..17029
                     /gene="dnaB"
                     /locus_tag="VBD89_001454"
     CDS             15674..17029
                     /gene="dnaB"
                     /locus_tag="VBD89_001454"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO7135972.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            17041..>17300
                     /locus_tag="VBD89_001455"
     CDS             17041..>17300
                     /locus_tag="VBD89_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO7135973.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIR"
BASE COUNT         5706 a         2704 c         3371 g         5519 t
ORIGIN      
        1 aaaatgggag taaatattgg ttgacgactt tgtcaatttt gaagaagatt gaaatttaga
       61 attaatcata tcttggatgt ttaatctttt ttaacgcctt taaaaatgat ataatcttag
      121 ctaacaatac agaacacaaa ggatattgaa tgaaaatagt tgaaggcgtt tctctacatt
      181 taattaaaaa ccaacaattt aagactaatc atcttacttt tcgattttca ggtgatttta
      241 ataataaaac agtagcacgt agatcgctcg ttgctcaaat gttagttact gcaaatgcta
      301 agtaccctaa ggtacaagaa tttcgagaaa agttagcttc cttatatggt gctagtttat
      361 caactaagat ttctactaag ggtcttgttc acattgttga tattgatatt gtatttgtta
      421 aaaatacctt tactttagaa caagaaaata tcgttgaaca aattattaca ttcttggagg
      481 atatgctgtt ttctccttta atttctttag aacagtatca aacatctatt tttgatactg
      541 agaagaaaaa ccttattcaa tatttagagg ccgatattga agataatttt tatagcagtg
      601 acttagcact aaaatcttta ttctacaata ataagacact tcgtttacct aagtatggta
      661 cagcatcttt agtggaatca gaaaattcat ttacagctta tcaagaattt cagaagatgc
      721 ttaaggaaga tcaattagat atttttgtcg taggagattt tgatgactat cgaatgattc
      781 aggcatttaa tcgtatggca tttgagccac gtcacaaagt attagctttt gattatacgc
      841 agacttatga aaatatcacg agaagtcaag tagaagataa ggacgttaat caatctatta
      901 tgcaattggc ttatcatctt ccaataactt ataaagatga agattatttt gctttaatag
      961 tatttaacgg tttatttggt gcttttgccc attccttgtt gtttacagaa attcgagaaa
     1021 agcaaggttt agcctatact ataggtagtc aatttgacag ctttacaggt ctctttacga
     1081 tatatgcagg tattgataaa gagaatcgtg agcgtttttt gaagcttatt aataagcaat
     1141 tcaataatat taagatgggc aggttctctt ctacattatt gaaacaaaca aaagatatat
     1201 tgaaaatgaa ctatgtttta gcttctgata atcctaaggt catagttgac cacatttatc
     1261 atgaacatta tttagatcag tttcatactt cagcactatt cattgacaaa gtggacgatg
     1321 taacaaaatc agatattgtc agcgtagcaa caaaattaaa attacaagct ttttattttt
     1381 tggaaggaaa ctaggatgac agttaataaa ataacttatc agaaccttca ggaggaagta
     1441 tataagttaa cattagagag cgggctaaat gtatatctta ttccaaaacc atcatttaag
     1501 gaaactgttg gagtattgac agcaaatttt ggatcactcc atacaagata tacgaggaat
     1561 ggttgtgtag aacattatcc ggcaggaatt gctcacttct tagaacataa attatttgaa
     1621 ctggataaag gacaagacgc tgcaactcaa tttactaaat atggcgccga gagtaatgcc
     1681 tttacaactt ttgataaaac tagtttttat ttttcaacta taagtcacat tacaaactgc
     1741 ttagatatac tacttgactt tgtattgaca acaaacttta cagaagagtc aattactaaa
     1801 gaaaaagaca ttatcaaaca agaaattgaa atgtatcaag atgacccaga atataggctt
     1861 tatcaaggag tgttatcgaa cctttacccc aactctccat tagcatttga tatcgctgga
     1921 gattaccaat caatctctca aataacctta accgatttac aagaaaatca caaagacttt
     1981 tatcagttgt ctaatatgaa tttagttctt gttggacaat ttagtccaca agaaattata
     2041 acttaccttc aaaaaaattc tcatttaaca aattactcac agaatattga ccgagattct
     2101 attagtcttg aacctgttat aaaaaacaac tcttgccata tgacagtgac aaaacctaaa
     2161 ttagctattg gttatcgaaa atcaagtcat atgagacatg gttcttacct aaaagagaaa
     2221 atcggattac agctattttt tgcaatgctt ttaggatgga cttctacaat taatcaagac
     2281 tggtacgaat ctggtcaaat tgatgactct tttgatatag aaattgaagt tcatccagat
     2341 tttgagtgcg ttattatttc tttagatacg acagagccaa ttgctttttc aactcagttg
     2401 aggttattgc taaaaaatgc tttgcaatca tctgatctta ccgaaagcca cttacaaaac
     2461 gttaagagag agctctatgg cgatttttta aggagcttag attctattga aaatctcgca
     2521 atgcaattcg tcacctattt atatgatgga aaaacgatgt atttagattt gccatctatc
     2581 gttgaagaat tagacttgga agatgtcatt acgattggta aggatttctt agataatgct
     2641 gacacatctg acttcgttat ttttccaaaa tcttcgtaaa taatctgaaa ttttgatata
     2701 ataaggaagc ttagacatct ataaagttgg tatgtcaaaa gtcttttact agttttgaaa
     2761 taacataata taggaatact atgtttccaa aaggagaatt tatggtgaag aaagaaaata
     2821 ttccgaatct attaactgta gtcagaattt taatgattcc gttatttata gtattaactt
     2881 cagtaacaac aagtacaact tggcatattg ttgcagctat tgtatttgct attgcaagct
     2941 tgaccgatta cctagacggt tatctagcac gtaaatggca agtagtaaca aattttggga
     3001 aatttgcaga tccattagct gataaaatgc tagttatgag tgcttttatc atgttagttg
     3061 gactagactt agcaccggct tgggtttcag ctattatcat ttgtagagag ctagcagtta
     3121 caggtttgcg tttactttta gttgaaacag gtggaacagt acttgctgca gctatgccag
     3181 ggaaaataaa gacggctact caaatgtttg cagttatttt tttgttagta cattggatga
     3241 cacttggtaa catcatgtta tacattgctt tattctttac cttgtattca ggctacgact
     3301 attttaaagg tgcaggcttc ttatttaagg atacttttaa ataatgacaa atattattac
     3361 tgttaataat ctctttttta aatatgatag taatcaaaca cattatcaat tggagaatgt
     3421 atcgtttcat gtgaaacaag gagaatggct atctattatc ggtcataatg gttctgggaa
     3481 atccactact gttcgtttaa tagatgggct tttagaggcg gagtcaggcc agattatcat
     3541 tgatggacaa gagttaaccg aagataatgt ctgggagtta cgtcacaaga ttggcatggt
     3601 atttcaaaat cctgataacc aatttgtagg ggcaactgtt gaagatgacg tcgcctttgg
     3661 tttggaaaat aaaggtattc ctttgaaaga catgaaagaa agagtggacc aagcacttga
     3721 tttggtaggc atgtctgaat ttaaaatgag ggagcctgca cgtttatctg ggggacaaaa
     3781 gcagcgtgta gccattgcag gagctgtagc tatgcgtcca caagttatca tcttggatga
     3841 ggcgactagt atgcttgatc ctgagggacg attggaactg ataagaacga tacgagctat
     3901 tcgtcaaaaa tataatctta ctgttatttc aattacacac gacttagatg aagttgcatt
     3961 gagtgatcgt gtcatcgtta tgaaaaatgg taaagttgag tcgacatcca ctccaaaagc
     4021 gttatttggt cgtggaaatc gtttgattag tttaggttta gatgttccct ttacaagtag
     4081 gttaatggca gaactggctg ctaatggtct tgatataggg acagagtatc ttacagagaa
     4141 ggaattagaa gaacaattat gggaattgaa tttaaaaatg taagttatac ctatcaagcc
     4201 ggcactcctt ttgaagggcg tgcccttttt gacgtcaatc tgaaaattga agatgtttcc
     4261 tataccgcgt tcattgggca cacaggttct ggaaaatcaa ctattatgca acttttgaat
     4321 ggtttacata ttcctacaaa aggtgaggta attgtcgatg atttttctat taaagcaggg
     4381 gacaagaaca aagaaatcaa atttataagg caaaaagttg gtttagtttt tcaatttcca
     4441 gaaagtcagc tttttgaaga gacagtttta aaggatgttg cttttggacc acaaaatttt
     4501 ggtatttctc agattgaagc tgaaaggctg gctgaagaaa aattaaggtt agttggtatc
     4561 agtgaggatt tattcgataa aaatccattt gaactttctg gagggcagat gaggcgggtt
     4621 gctatagctg gtattttagc gatggaaccc aaagtactag tactggatga gccaacagct
     4681 ggacttgatc ctaagggaag aaaagaatta atgactcttt ttaaaaatct tcataaaaaa
     4741 ggaatgacta tcgtcttagt gactcactta atggacgatg tagcggatta tgctgactat
     4801 gtgtatgttt tagaagcagg gaaagtaacc ttatcaggac aaccaaaaca gatttttcaa
     4861 gaagtagaac ttttagaaag taaacaatta ggagttccca aaatcaccaa gtttgctcaa
     4921 agactatctc ataagggatt aaatttacct agtttaccaa ttactattaa cgaatttgtg
     4981 gaggctatta agcatggata aattgatttt gggacgttac ataccaggta attctctcat
     5041 tcataaatta gatcctcgaa gtaagttact agccatgttg ctttttatca ttattgtttt
     5101 ttgggcaaat aatgtagtga caaatgtgat cgtattcatt tttacgttgg ttattgtggg
     5161 tttatctcag ataaaatttt cctatttttt caatggtatt aaaccgatgg ttggtattat
     5221 tttatttaca accctgttcc agatgctgtt tgcacaaggc gggcaggtta tcttctcgtt
     5281 ttggattttt agcatcacta gccttgggtt acaacaagca gcacttattt ttatgagatt
     5341 tgttttaatt atcttctttt cgacattact taccttaact actactccgt taagtttagc
     5401 agatgctgtt gaatctttgt taaaaccatt ggaagtactg agagtaccag cacacgagat
     5461 tggtttgatg ttatccctta gcttacgttt cgttcctact ttaatggatg atacaacacg
     5521 tatcatgaat gctcaaagag ctcgtggagt tgattttgga gagggaaacc taattcataa
     5581 ggttaagtcc attatcccta ttttgattcc attatttgct tctagtttta aaagagcaga
     5641 tgcgttggca attgctatgg aagcaagagg ttatcaaggt ggagccaatc gtagtaagta
     5701 ccgtttattg aagtggactg ttcgggatac ttttagtata ttactgatgt tattattggg
     5761 tttgagtttg tttctattaa aaaattaaaa ggttaaggtc agtttatggt cttagccttt
     5821 tcatttatat aatgtgaaaa gctcaacaac tttacttaaa gaaaactaaa aattgttttt
     5881 tggaggcctt ttctttatta atttacaatt tatttgtaat aaatttcatc taaggcaaaa
     5941 caattagtaa tatttacaag gtatgatagt acgcgtatca attaatgaaa gagagcatat
     6001 attatgtcaa taacctcggt taaaaaatca aaaccattta aattaggagt ggcaggtctt
     6061 ttagtgggtg cttcattagc tttaccactt tcagtaagcg cagcatctta taccgtgaaa
     6121 tcaggtgata ccttatcagc tattgctaaa aatcataaaa ctacggtaca atagttagtg
     6181 tctctcaata gtatcagtaa cgctgatgtc atcagtatag gtgatgtttt aaaattggat
     6241 aattctacag ctagtcaagc agaagcaaaa tctcaaccaa caattgaaaa ttcaatgaat
     6301 tcttcatcaa atttgagttc aagtgattca gctgcaaaag aagaaatagc tcgtcgtgaa
     6361 tcaaatggta gttatactgc acagaatgga caatattatg gaagatatca actgtctcaa
     6421 tcttacctaa atggcgactt atctcctgaa aatcaagaaa aagtagcgga caattatgtg
     6481 gcttctcgtt acggatcttg gtcggcagcg ctatcatttt ggaatagtaa cggttggtat
     6541 taataaaagt tgataaaaca aagagattta cttaaaatta aatctctttg taatattttt
     6601 aacatctatt caattgcaag gtaacaaata aagtataata taggtaacat gaataaaaga
     6661 agaaaattat caaaattgaa tgtaaaaaaa caacatttag cttatggagc tatcacttta
     6721 gtagcccttt tttcatgtat tttggctgta acggtcatct ttaaaagttc acaagttact
     6781 actgaatctt tgtcaaaagc agataaagtt cgcgtagcca aaaaatcaaa aatgactaag
     6841 gcgacatcta aatcaaaagt agaagatgta aaacaggctc caaaaccttc tcaggcatct
     6901 aatgaagccc caaaatcaag ttctcaatct acagaagcta attctcagca acaagttact
     6961 gcgagtgaag aggcggctgt agaacaagca gttgtaacag aaaatacccc tgctaccagt
     7021 caggcacaac aaacttatgc tgttactgag acaacttaca aacctgctca acaccagaca
     7081 agtggccaag tattgagcaa tggaaatact gcaggggcgg tcggatctgc tgctgcagca
     7141 caaatggctg ctgcaacagg agtccctcag tctacttggg aacatattat tgcccgtgaa
     7201 tcaaatggta atcctaatgt tgctaatgcc tcaggagctt caggactttt ccaaacgatg
     7261 ccaggttggg gttcaacagc tacagttcag gatcaagtta attcagctat taaagcttat
     7321 cgtgctcaag gtttatcagc ttggggttac tagtgaatag ctataaatta actttacact
     7381 attgtcaata ataattacag attgctaaaa caaataaata aaaacgctac agtaaagctg
     7441 tagcgttttt atttattttc agggaaagag tagaaaattg ataaacttta ttccccaaaa
     7501 atttctttgc tataacgtct tcctgtcggc gtggcagcaa gtcctccttc tgcagtttca
     7561 cgaaaagcag tcggtaaact tgatccaact tgatacattg catcaataac ttcatctact
     7621 ggaatttgcg attcaatacc agccaaggcc atatcagcag caacaagtgc aaaacttgat
     7681 ccaagagcat tccgcttcac acaagggact tcaactaaac ctgcaacagg gtcacagata
     7741 agtccaagca tatttttaat aacaaatgct atagcttggc tagcttggaa aggagtacct
     7801 ccagcagcca taactaaagc agccgcagcc atagcactag ctgacccaac ttcagcttgg
     7861 caacctcctt ctgcacctga gatagaggca ttattaccaa tgacaagacc aaatgcgccg
     7921 gctgtaaata gaaaattaag ttgctcttct tctgttaaat taagcttttc aatggctgta
     7981 gaaatcacag ctggtaaaca tcctgcacta cctgcagttg gtgttgcaca gaccagtccc
     8041 atcttagcat ttaactcatt aacagccata gcattcctaa cggcagctag gattgtggta
     8101 tctgaaatag tttttcctga ttgtaaatat tgatccatct tgacagcatc accgcctgtt
     8161 aaaccactga ttgatttact aggggtcaat ccatcaataa cagaagcttt cataacctca
     8221 aggtttcggg acataatata acgaatttct tcacgacttc taccagtcat ttcaatttcc
     8281 gtttggatca tgagctctgc tatgttaccc ttatgttggc tattagcttg ctctaccagc
     8341 tcttcaattg tataaaacat agttaccctt tctcttaatc aaagaagttg acattgtgta
     8401 aatgaggaat agcttctatc tttttgacag cttgttgaca atctcgtgaa tctacttcta
     8461 taatcatgat agctttttct cctgcacttt cgcgtgttac attcatttga gcaatattaa
     8521 tgttaaagtc agataaaata tcagttactt tagcaatcat gccaggtata tcttgatgaa
     8581 cgataattaa agtaggagta ttcattgtta atgaaactga gaatccattc aactcagtaa
     8641 cttggatgtt tccgccacca attgacactc ctgtaatact catgctccta tccccatttt
     8701 taacagttat tttcgcagta tttggatgtg gtgaattact atcttttagg atatcccagt
     8761 aaatcttaat tcctttttga tgagcaattt caagagagtt tttaatatct ggattatctg
     8821 tatccattcc tagaatccct gcaaccaatg ctttatcagt accgtgtcct tggtaagttt
     8881 tagcaaaaga attgtataaa tgaaaggtta cttcactagg ttcaccaaaa atagaatgga
     8941 caactttacc aatgcggaca gctcctgcag tatgactact tgatggtcca atcataacag
     9001 gaccaataat gtcgaagacc gattgaaatt ttaaatgttt catagttttt cctcgtgatt
     9061 tttgtacatt ctcattataa cagattattt ctaaaaaata tcataaaaag ataaaaaaac
     9121 tgcaatgtaa taaatattta tcacaacaaa aacatacaac ttgacaacaa atagtgctat
     9181 aatatataag cacttttata gtgtttctta gtcaatatga ttgatctaag aacaacttgg
     9241 cccttcaagc ctaagttaaa aataaaactt tttgatggga gaattcttat gactgataat
     9301 tcaaacattc gtgtcgttgt aggcatgagt ggaggtgttg attcatcagt aactgccctg
     9361 cttttaaaag agcaaggtta tgatgttatt ggtgttttta tgaaaaattg ggatgacaca
     9421 gatgaatttg gtgtttgtac ggctactgaa gattataaag atgttgctgc tgttgctgat
     9481 cagatcggca taccatacta ctctgtcaac tttgaaaaag agtattggga cagggttttc
     9541 gaatattttt tagcagaata tcgagcaggt cgtacgccaa atccagatgt tatgtgtaat
     9601 aaggaaatta aatttaaagc atttctagat tatgcgatga cattaggtgc tgactatgta
     9661 gcaacaggtc attatgcaca agtaacacgt gatgagaacg gaatagttca tatgcttaga
     9721 ggagcagata ataataagga tcaaacttat tttctaagtc aattatctca ggagcaattg
     9781 caaaaaacgt tatttccact tggtcattta caaaaaccag aagttcgccg tatagctgaa
     9841 gaagcaggac ttgcgaccgc taagaaaaaa gactctactg gtatttgttt tattggagaa
     9901 aaaaatttca aggatttcct gggtcaatat ttaccggctc aaccaggtcg tatgatgact
     9961 gttgatggtc gtgacatggg tgaacatgct ggtttaatgt attatactat tggtcaaaga
    10021 gggggccttg gtattggtgg gcaacatggt ggagataata aaccttggtt tgtagttgga
    10081 aaggatttaa gtaaaaacat tctttatgta ggtcaaggtt tctatcatga ctcattgatg
    10141 tcaactagct tgacagcttc tgaaatccac tttacacgtg atatgcctaa tgaatttaag
    10201 ctagaatgta cagctaagtt cagatatcgt caacctgaca gtaaagttac agtttacgta
    10261 aaaggtaatc aagcaagggt tgtgtttgat gatttacaac gagcaatcac accaggtcaa
    10321 gctgttgttt tttataatga acaagaatgt ttaggcggtg ggatgattga tcaggcttat
    10381 cgtgatgata aaatctgtca atacatttaa ttgacactta attattaagt gaggtaatcg
    10441 tatgatttca aaatttattt tagctttcat ggcttttttc gccattatga atcccatttc
    10501 taatttgcca gcttttatgg ctttagtagc tgatgatgat cagaagattt cacgtcggat
    10561 tgcagcaaaa ggagtattac ttgcttttgt cattattgta atctttgtat taagtggcca
    10621 cttacttttt aacttatttg ggattaccct agccgcccta aaaatatctg gtggaatatt
    10681 ggtaggcatt ataggttata aaatgattaa tggtatacat tcaccaacca ataaaaacct
    10741 agaggagcat aaggatgatc caatgaatgt ggcagtttca ccattagcaa tgcccctttt
    10801 ggcaggtccg ggtactattg caactgctat ggggttatca agtggtggtt tatctggcaa
    10861 actcattact atacttgctt ttgccatatt atgtgtgata atgtatgtta ttttaataag
    10921 tgcaaacgaa attactaaat ttcttggaaa aaatgcaatg actataatca ccaaaatgat
    10981 gggacttatt ttgatgacta tcgggataga gatgctaata acaggcataa aaattggttt
    11041 tcatttaaca tagcctatcc cctctggttg acttttaaaa gataaatgct aaaataaatt
    11101 taacaataac tatgatggtc aaagctcatg gaatttgtaa gtctttttga cgtacaattt
    11161 tcgagagttt tggctatttt tgtgttttaa tgaattagac tttgaaattg gaaaggaata
    11221 aaatgacaca taattttgca gaaaactatg atattatcgt tgttggagcg ggtcatgcag
    11281 gtgtggaagc tagtctagct gctagtcgta tgggatgtaa gacacttcta gccactatca
    11341 acttagaaat gttagcattt atgccttgta atccttctat aggtggctct gctaaaggta
    11401 tcgttgtcag agagattgac gctttaggtg gtgaaatggg taaaaacatt gataaaacct
    11461 atatacaaat gaaaatgctt aatacaggta aggggcctgc tgtccgtgcc ttaagagcac
    11521 aagctgataa agccctctat gcacaaacaa tgaagcaaac tgtagaaaag caagaaaatc
    11581 ttaccttaag acaagctatg attgacgaaa tacttgttga agatggaaag gttgtggggg
    11641 ttcgtacagc aactaatcag aaattttcag caaaatctgt tgttataaca acaggtactg
    11701 ctcttcgagg agagattata ttaggtgatc ttaagtactc ctcgggccct aataatagtt
    11761 tagcttctgt gacattggca gataatttaa gagacctagg attagaaatt ggtcgtttca
    11821 aaactggaac acctcctcgt gtaaaagcct cctctattaa ttatgaaaag acagagatac
    11881 aacctgggga tgagcaacca aatcattttt catttatgtc tagggatgaa gattatatta
    11941 ctgaccaggt accgtgctgg ttgacctata cgaatacttt gagtcatgac atcatcaatc
    12001 aaaatctaca ccgtgctcct atgttttctg gaatcgttaa gggagttggt cctagatatt
    12061 gtccgtcaat tgaagataaa attgtgcgtt ttgctgataa agagcgccac caactcttct
    12121 tagaaccaga aggtagatac actgaagaag tttatgttca agggctatca acgagtcttc
    12181 ctgaggatgt tcaagttgat ttattacgtt ctataaaagg gcttgaaaat gctgaaatga
    12241 tgcgtacagg ctatgctatt gagtatgata ttgtattgcc tcatcaatta agagcaacac
    12301 tcgaaacaaa agtaattgct ggtttgttta ctgcaggaca gactaatggt acttcaggtt
    12361 atgaagaagc tgctggtcaa gggttggttg ctgggatcaa cgctgctctt aaagttcaag
    12421 ggaagccaga acttatttta aaacgaagtg atgcttacat tggtgtcatg attgatgatt
    12481 tagttaccaa agggactcta gaaccatatc gtctgttgac aagtcgcgca gaatatcgtc
    12541 tgattcttcg tcatgataat gcagatatgc gtttaacaga aatcggttat gagattggcc
    12601 tagtagatga agagcgttat gcgatattta aaaagcgtca aatgcaattt gaaaatgaat
    12661 tagaacgttt agactctata aagttaaagc cagtttctga gacaaataaa cggattcaag
    12721 aattgggctt taaacctttg acagatgctt taactgccaa agaatttatg cgccgcccac
    12781 aaatcacata tgcagtagca acggattttg ttggttgtgc tgatgagcca ttagacagta
    12841 aggttattga acttttggaa actgaaatta aatacgaagg ctatattaaa aaggctttag
    12901 atcaagttgc aaaaatgaaa cgaatggaag aaaaacgtat tccccctcat attgactggg
    12961 acgacattga ttctatcgca acagaagcta gacaaaaatt caaaaaaatt aacccagaaa
    13021 cgctaggtca ggctagtcgt atttcaggag ttaatccagc cgatattagt attttaatgg
    13081 tttaccttga aggaagacaa aaaggtcgta aaaatatcaa ttgataatag tgttcgcccc
    13141 tgtttatcag gggcgttttg tgttataatt aggtcttaag aggttattta atgaaaagat
    13201 ttcgttttgc aaccgtccat ttagttttaa taggcctgat tttatttgga ctattagcta
    13261 tttgtgttag gttatttcaa tcctatacgg ctctattatt ggctattttt gttgctttat
    13321 cttttgttgt tgccttatta tattaccaaa agataactta tgaactatct gaagttgaac
    13381 aaattgaatt acttaatgat caaacagaag tttctcttaa gagtcttctt gaacaaatgc
    13441 cagttggtgt tatacaattt gatcttgaaa caaatgatat tgagtggttt aacccttatg
    13501 cagaattgat ttttactggg gataatggtc attttcagtc tgctactgtt aaagatatca
    13561 ttactagtag acgtaatgga acggctgggc aatcatttga atacggtgat aacaaatatt
    13621 cagcttattt ggatacagag acgggtgttt tctatttctt cgataatttc atggggaata
    13681 gaagaaacta tgatagttca atgctacgtc cagttattgg tattatttcc attgataact
    13741 acgatgatat tatggatact atgttagagg ctgatatgtc aaagattaac gccttcgtga
    13801 ctagttttat ttctgatttt acacagtcta aaaatatttt ttatcgtcgt gtcaatatgg
    13861 atcgatacta tatttttacc gactatagcg ttttaaatac attgataaaa gataaatttg
    13921 atattttaaa tgagtttcga aaacgcgctc aggaaaacca tttatcatta acattaagta
    13981 tgggaatttc ttatggtgat ggtaaccata atcaaattgg gcaaattgct ttagaaaatc
    14041 ttaatacagc actcgttcgc ggaggagatc aaatagtagt acgtgaaaat gatagtagta
    14101 aaaaagcact ttactttggt ggtggtgctg tttcaactat taaacgttca cgaacacgta
    14161 ccagagctat gatgactgct atttctgacc gtttgaaagt agttgatagt gtctttattg
    14221 tgggacacag gaaattggac atggatgcac taggtgcatc tgtagggatg caattttttg
    14281 ctagtaatat tgttaatgcc agttatgtag tttatgaccc aaatgacatg aattctgatg
    14341 ttgaaagagc aattgattac ttgcaagaag acggcgaaac acgtctcgta agtgttgaga
    14401 gagcttttga attaattact caaaattctt tattagtcat ggtagaccat tctaaaacag
    14461 ctttaacctt atctaaggaa ttctttaaca agtttgcaga tgttattgtg gtagatcatc
    14521 atagaagaga tgaagatttt ccaaaaaatg ctgttctttc atttatagaa agtggcgcaa
    14581 gtagtgccag cgaattagtt acagaattga ttcaatttca acaagctaaa gataaattaa
    14641 gccgcagtca agcaagtatt ttaatggctg gtatcatgct tgatacaaga aattttgcat
    14701 ctaatgtaac gagtagaact tttgatgtgg caagctattt acgcggttta ggtagtaata
    14761 gtatggctat tcaaaaaata tcagctacag attttgatga atatcgttta attaatgaat
    14821 tgattttaaa aggtgagcgt atttatgata acatcattgt tgctaccgga gaagaacata
    14881 aagtttactc gcatgttata gctagtaaag cagctgacac catgttgaca atggcaggta
    14941 ttgaggctac ttttgttatt actaaaaaca gttcaaatat tggtatttca gcgcgcagta
    15001 gaaataatat caatgtccag cgtattatgg aaaaacttgg tggtggcgga cattttagtt
    15061 ttgctgcttg tcaaatacaa gacaagtctg taaagcaagt tagacgtatg ttactggaaa
    15121 taattgacga agatttaaga gaaaatagta cagtcgaaaa tagaagggat taattgagat
    15181 gaaagttatt tttttacaag atgttaaggg gaaaggaaaa aaaggtgagg ttaaggaagt
    15241 acctactggt tatgctcaaa acttcttatt aaaaaagaat ctagctaaag aagcaacgac
    15301 acaagctatc ggtgaattaa aaggaaaaca aaagtccgaa gaaaaagcac aagcagaaat
    15361 tttagcacag gcaaaggagc tgaaaacaca attagaatcc gaaactaccc gagtgcaatt
    15421 tattgaaaag gtaggtccag atggtcgtac gtttggttct atcacagcca aaaagattgc
    15481 tgaagagtta caaaaacaat acggcattaa aattgacaaa cgtcatattg atttagatca
    15541 tactattaga gcgattggta aggttgaagt accagtaaaa ttacataaac aggtttcttc
    15601 acagattaag cttgatatca aagaagcgta aactgagaga tgataaaggt tgacaagtca
    15661 ggaggaatga agtttggtag aagtatctga gctaagagta caacctcaag acctgttggc
    15721 agaacaggcc gttctaggat ctatctttat ctcaccagag aaactgatta tggtgagaga
    15781 atttataagt ccagatgatt tttataaata ttcacacaaa gttatcttca gagcgatgat
    15841 taccttagct gatagaaatg atgcaattga tgctgctact gttagaaata ttttagatga
    15901 ccaaggtgat cttcaaaaca ttggtgggtt gggctatatt gttgaattag ttaatagtgt
    15961 tccaacaagt gctaatgcgg agttttatgc caaaatagtt tctgagaaag ccatgttgcg
    16021 agatatcatt tctaagttga cagatactgt caacatggcc tatgaaggaa atgattctga
    16081 tgaaattatt gccaccgctg agaaggcttt agttgatatt aacgaacaca gtaatcgtag
    16141 cgggtttaga aagatatctg atgttttaaa agttaattac gaaaacttag aattacgttc
    16201 acagcaaacc tcagatgtca caggtttacc aactggattt agagatttag acaggattac
    16261 tacaggttta catccagatc aattaatcat cttagcggct cgtcctgcag taggtaaaac
    16321 agcctttgtt ttaaatattg ctcaaaatgt tggaactaaa caaaatagac ctgtagctat
    16381 tttttcactt gaaatggggg ctgagagttt agttgaccgt atgttggcag cagagggaat
    16441 ggttgattct catagtttac gaactggtca gttgacggat caagattgga ataatgtaac
    16501 gattgctcaa ggagctttgg cggatgcgcc aatttatatt gatgatacgc ccgggattaa
    16561 aattacagaa attagagcac gctctaggaa gttatctcaa gaagttgatg atggtttagg
    16621 tttaatcgtc attgactacc tccaattaat ttcaggaact agaccagaaa atcgtcagca
    16681 agaagtttct gaaatttcaa ggcagttgaa gattttagct aaagaattaa aggttcctgt
    16741 tatcgcatta agccagctct ctcgtggggt tgagcaaagg caggataaac gaccggtctt
    16801 atctgatatt cgtgaatctg gatcgattga acaagatgct gacattgttg ctttcttata
    16861 tcgagatgat tattatcgta gagagggcga agaagcagag gaaattgtag aagataatac
    16921 tgttgaggtt attttagaaa agaaccgtgc tggtgcgcgt gggacagtaa aattaatgtt
    16981 tcaaaaggaa tataataaat tttctagtat agctcaattt gaagaatagg agatgttaat
    17041 atgagtgatg catttgcaga tgttgcaaaa atgaaaaaaa taaaagaaga tatcaagtca
    17101 catgagggac agatggtcga acttactctc gaaaatggtc gtaagaggga aaaaaataaa
    17161 attgggcgcc taattgaagt ttacccatct ttgtttatcg tagagtataa ggatactgca
    17221 gctgtaccag gagcaattga taatacttat gtagagtctt acacttattc agatatttta
    17281 actgagaaaa ctttaattcg
//
LOCUS       DATRSG010000048        16800 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRSG010000048 DATRSG010000000
VERSION     DATRSG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16800
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.048"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..230)
                     /locus_tag="VBD89_001456"
     CDS             complement(<1..230)
                     /locus_tag="VBD89_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135974.1"
                     /translation="MLANNKKRKRKFTLFLLMIIYRTFIKSYNKLVKYRKYCIFLENM
                     VYIVNKMMTKLIEFSYRKIFQKNNFMLKSFDC"
     gene            complement(217..1413)
                     /locus_tag="VBD89_001457"
     CDS             complement(217..1413)
                     /locus_tag="VBD89_001457"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO7135975.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1598..2533
                     /locus_tag="VBD89_001458"
     CDS             1598..2533
                     /locus_tag="VBD89_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO7135976.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2514..2708)
                     /locus_tag="VBD89_001459"
     CDS             complement(2514..2708)
                     /locus_tag="VBD89_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO7135977.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2797..4461)
                     /gene="dnaX"
                     /locus_tag="VBD89_001460"
     CDS             complement(2797..4461)
                     /gene="dnaX"
                     /locus_tag="VBD89_001460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO7135978.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4461..4958)
                     /locus_tag="VBD89_001461"
     CDS             complement(4461..4958)
                     /locus_tag="VBD89_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO7135979.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(5045..5674)
                     /gene="udk"
                     /locus_tag="VBD89_001462"
     CDS             complement(5045..5674)
                     /gene="udk"
                     /locus_tag="VBD89_001462"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO7135980.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            5819..6901
                     /locus_tag="VBD89_001463"
     CDS             5819..6901
                     /locus_tag="VBD89_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7135981.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(6933..8186)
                     /locus_tag="VBD89_001464"
     CDS             complement(6933..8186)
                     /locus_tag="VBD89_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO7135982.1"
                     /translation="MKKLIIGIFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIA
                     EKTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLAD
                     SNFANVKKLSIYQILYQKEKGSMFQKSSRLLRTYLLDQNKKPFELDELLAHNISGFKA
                     ILENIAPGTQLKEHDSNKEFLKTGRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYL
                     KSSDLDLVSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFIMGQSAVGHT
                     DILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYG
                     ATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTSIDA
                     LPTIMDYLTTQGYYFVTVGELYSTP"
     gene            complement(8326..9753)
                     /locus_tag="VBD89_001465"
     CDS             complement(8326..9753)
                     /locus_tag="VBD89_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7135983.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(9903..11636)
                     /gene="ptsP"
                     /locus_tag="VBD89_001466"
     CDS             complement(9903..11636)
                     /gene="ptsP"
                     /locus_tag="VBD89_001466"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO7135984.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(11641..11904)
                     /locus_tag="VBD89_001467"
     CDS             complement(11641..11904)
                     /locus_tag="VBD89_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO7135985.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
     gene            12287..12511
                     /locus_tag="VBD89_001468"
     CDS             12287..12511
                     /locus_tag="VBD89_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO7135986.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            12589..14748
                     /gene="nrdE"
                     /locus_tag="VBD89_001469"
     CDS             12589..14748
                     /gene="nrdE"
                     /locus_tag="VBD89_001469"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7135987.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            14951..15910
                     /gene="nrdF"
                     /locus_tag="VBD89_001470"
     CDS             14951..15910
                     /gene="nrdF"
                     /locus_tag="VBD89_001470"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7135988.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      15995..16093
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16150..16713
                     /gene="thiT"
                     /locus_tag="VBD89_001471"
     CDS             16150..16713
                     /gene="thiT"
                     /locus_tag="VBD89_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO7135989.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
BASE COUNT         5394 a         3183 c         2713 g         5510 t
ORIGIN      
        1 tgacaatcaa atgatttgag catgaaatta tttttttgaa aaatttttcg ataggaaaat
       61 tcaattaact tggtcatcat tttattcaca atatacacca tattttcaag aaatatgcag
      121 tatttcctat acttaaccag tttattataa gattttataa atgttctata aattatcatg
      181 agcaaaaaaa gcgtaaactt tcgtttacgc tttttattat ttgctagcaa taaaatcctt
      241 taaggcctgg actttatcaa ctcgctccca tggcaaatcg atatcagtac gtcccatatg
      301 cccataggct gctgtttgtc gataaatagg tcgtttgagg tccaacatat taataatgcc
      361 tgctggtcgc aaatcaaata tttggcgtac agctgcctct aggtctgctt ctgctataac
      421 acctgttcca aatgtatcta cacgcacaga gacaggctga gcaacaccaa ttgcataagc
      481 aagttgtact tctacttttt tagctaaatc agctgcaaca atattttttg caatataacg
      541 agctgcataa gatgctgaac gatctacttt agtagcatct ttacctgaaa atgcaccacc
      601 accatgacga gaataaccac cataagtatc tacaataatc ttacgacctg ttagtcctga
      661 atctccttga gggcctccaa taacaaagcg ccctgtagga ttgataaaga attttgtttg
      721 gtcatctaaa taatggcttg gaataacttc attgataact ttctcaatca cgtccttatg
      781 gagttggtca ttagttacat taggatcatg ctgtgtcgaa ataactactg catccacacg
      841 aattggttga tcattatcat catattcaac cgttacttga gattttgcat ctggtcgaag
      901 ataggtgagt tccccagact tacgtagatc tgtcaatttc ttaactaact gatgagctaa
      961 agaaattggc aatggcatta actcaggtgt ttcatcaaca gcaaacccaa acatcaaacc
     1021 ttggtcacct gcaccaatta aatctaaagg atcttgttca agagaaccac gtacctcaag
     1081 agcttcattg actccctgtg caatgtctgg cgactgttca acgagtgacg gatgcactcc
     1141 gacagattca gctgagaagc catattcagc tttatcatag ccaatttcag cgattgtgtt
     1201 acggacaact cggttaatat caacataggc tgtcgttgat atctcgccaa atacatgaac
     1261 cgaacctgta taaacagctg tttcggctgc tacgtgggca tctggatctt gctctaaaat
     1321 tgcatctaaa atcgcatcgg aaatttggtc agcaatctta tcgggatgtc cctcagatac
     1381 tgactcagac gtgaaaagtt tacgttctga cataaaaatg tcccccctaa aatgtaaaat
     1441 gtgtcgagtt acaaagaact agcctctagg ctaccttttc gggactcata tagctattgt
     1501 accactattt catcttttgt gaaataagtt accaataaat aaaaaattat ctctattttc
     1561 tttagtcttt tttctcgatt tatactatac tagatttatg aaaacctacg aaaaaattta
     1621 tcaaatacta tcaaaaaata acaactttat tagcggagaa actatggcaa atcaacttaa
     1681 tatttcccgc acagctattt ggaaaggtat aaaaacgtta gaagaattag gcctagagat
     1741 tgagtctgtc actaacaagg gctaccgatt ggtatcaggt gacatattat taccagaaca
     1801 actcgaacaa gaaattggta ttaaggttag tcttaataat aatagtgctt caacccaact
     1861 agatgctaaa atggggatag aaagcaaatt aaagactcca cacctttttt tagctcctaa
     1921 tcaaaaaaaa gctaaaggac gttttgatag acctttcttt acatcaaatc aaggcggcat
     1981 ctatatgtct cttttattac aacctaatgt accgattgaa gatataaaac cttatactgt
     2041 catggtagcc tctagtgctg taaaagctat ctcacggtta acaggaatta ctcctgaaat
     2101 taagtgggtc aatgatattt atttagataa taaaaaaata gctggtattt taaccgaggc
     2161 tatcgcttct gttgagagtg gattggtaac gaatgttatt ataggattag gtatcaattt
     2221 ctatatcaaa gagttcccta gggcgttaac taagcgcgca ggtagtctct ttacagagca
     2281 accgaccata actagaaatc aattgattac agaaatctgg aatctctttt tcaatattcc
     2341 actagaggat cacttaaagg tatatagaga aaaatcccta gtcttagata aaaccgttag
     2401 ctttatggat ggtcaaacaa tgtattctgg gaaagctatt gatattactg ataaaggata
     2461 tttagtggtt gagttagatg atggtcagtt gaaaacactt cgcagtggcg aaatcagtct
     2521 ttcttcttgg tagatttgat aagctctttt gtgaattttg aaatcgcata caccttttga
     2581 agatctcgaa gtaataaaac tccccaaaaa atagcaaaaa tccaggagcc atccataacg
     2641 gattgattga aaaaataagt ttcaaaagag cataaaagcg ccataaaaat aaattgtcgt
     2701 tttgtcatat caccattata acagaatcta ctaaataatt aaaaaactta tcaaaaaaat
     2761 agttgcctgt tacgggcaac tatttttagt tacgttttaa tcttcaacga ttgctattcg
     2821 ttctgctaga taagaaaagt cttctggaat ctgatgatta acttcaggtt ctgtttccgt
     2881 cttttgagct ttcattttct ttgcaaaatc gctccgaatt tgattaaaat catttctagg
     2941 tacagctaaa atattaggtg aaaaacctgc tgctttactc ataatattac caaatatatc
     3001 attgagatcg gtacgtttca tagcttgttc agcattaaag gccgcatcaa atgccaaaat
     3061 agcattctcg ctattagcta agacaggttc agatcccatt aataaggctc tatcttgagc
     3121 cgtaatatta tctaatatct cgttccaagc actctttaaa gcttctaaat attcacgtga
     3181 acgttggcta tctacaaccg tttcttccat aatggtcaaa atctttgtac ggtcaacttt
     3241 gaatgtgaat gtcttattat taacctttac tttttgagtt gatgaacttt gatctgctcg
     3301 agagagttgg ctcatttcgt tttttaacga ttttaactct cgacgaagac tatctaattc
     3361 tgctgtcaca tctgcgggta tgtttgacga atttttttct accatttcag acaattgaat
     3421 ggtcatcatc tctgcataaa ctttaggatg gctaccattt tttatctcag gaagaacaga
     3481 tgtcacttta tcaatcattt caaataaaat atcttgttta acatcaagat tggccataaa
     3541 agtatcactt gaatgactat tttcaccacc tgcttgaaca acaagtaaat ctcttaagta
     3601 catcaataaa tctgttgcaa aacgactcat acttttgcca ctatcaaaga ttacttcaag
     3661 tttagcgagt gcttcagttg tatcgtgagc caatatattc gatacataat catctatagc
     3721 agataaggaa atgctccctg taatttcttc tgctacatct aaagaaatgt ggttatcttt
     3781 cgctaaactc aaggcttgat cgagaattga taaagcatca cgcataccac cttcggctcg
     3841 tctagcgact agtgtcagag catccaaatc atatgaaata gcttctttat ctaaaatctg
     3901 tgctaaatga tctcgaatag ctaataattt aatcgcttta aattcaaagc gctggacacg
     3961 agataagatt gttgcaggga ttttatggag ttctgttgtt gctaatataa aaacaacatt
     4021 ttctgttggc tcttcaagtg ttttcaatag tgcattgaag gcacctgtag acaacatatg
     4081 aacttcatca ataatataaa ctttataggt tgccctactt ggagcataag ttgatttgtc
     4141 cctgatatca cgaatttcat cgacaccatt atttgacgca gcatcaattt caatgacatc
     4201 ctctaaacta ccatttgtaa tatctcgaca aatatcacaa tgattacaag gttcgccatt
     4261 aacttgattt gggcaattca tcgcttttgc aaatatttta gcagcacttg tttttccagt
     4321 tcccctaggt cctgaaaaaa ggtaagcatg gctaattttt ttgctggaca cagcttgctt
     4381 taaagtagtt gaaataacac tttgtccaac catttcatca aatgtttggc tacgatattt
     4441 ccgataaagg gcctgataca ttacttttca actccaaaca tatccaaatt ccaaatcgta
     4501 tgttctacta gaatacctac aaatttttct aagtattctt gatcaatctc atcataatct
     4561 gctactaaag aagaatctaa atctagaact cctagaagtt tgccattttt aaacatgggt
     4621 actacgattt cactcatagc ttttgaatca caggagatat agttagcatg ctttgtaaca
     4681 tcatcaacaa tcagcgtctt agcagtttgt gcagattcac cacaaacacc ttttcctaaa
     4741 gtaatatgca cacatgatac accaccctga aaagggccaa gaattaactc ctctccatca
     4801 aataaataaa agcctgtaaa tacagaattt ggaagcatag catttagcat agctgaagca
     4861 tttgataagt tggcaagagc atttgtttca tctgagaata gagcttgcgc ttgtagtaat
     4921 aataattgat aattttcgat tttcttagac ttgttcatag cacttattat agcataattt
     4981 gaaagttact aaaactaaat aaccatccta tataaaacaa aaaaataaac acaaaccatt
     5041 gtgtttattt tgcttcattt aagatgctag ccactttggt attgattaaa tcaatagcga
     5101 caatattact tactccttct ggaattacaa tatccgcata acgctttgtt ggttcgataa
     5161 attgatgata cataggtttt acaacttctg tatactgttc aataatacta tctaaacttc
     5221 tatcgcgctc ttccatatca cgcttaatac gacgaataat acgaatatca tcatctgtat
     5281 caacaaataa tttgatatcc attaaatcac gcaaacgctg atcttctaat accaaaattc
     5341 cctcaacaat aatgacatcc tgaggttctt ggcggatagt tctatcacta cgggtatgtt
     5401 ttgtgtaatc ataaaccgga atatcgaccg gacgcccctc aatcaattcg ttcaattgct
     5461 caatcatcaa attggtatca aaagcaaggg gatgatcata attggttttt acacgttctt
     5521 caaaagtcaa atgactttga tctttatagt aagaatcatg ttcaatcatg ataatttttt
     5581 gatctgggaa atttgataaa atcgcccgag aaacactggt tttaccacca ccagatccac
     5641 ctgtaacacc aataataata ggttttttac gcatttgtca ctccaaatta aatttatcct
     5701 ctatctattg tactataaac ttattttttt taaagtctaa attttattga aaacgctcta
     5761 aaaataaaaa gcatgttata ataacaatga caacatataa aataacaaga ggattcttat
     5821 gataacaaaa tttccagacc aatggcagga taaattaacc cagcgtcaat ttgatgatct
     5881 aacagatatt caaaataaac tttttcaacc tattactgat ggtgataata tccttggaat
     5941 tagccctaca ggtactggta aaactctagc ataccttttc ccaacgcttc ttaagttgca
     6001 acctaaaaag agccaacaac tcttaatttt agctccaaat tcagaattag ctggacaaat
     6061 cttcgacgtt acaaaagaat gggctgaacc attaggactg acagcacaac tattcctttc
     6121 agggtctagt caaaaacgcc aaattgagcg actaaaaaaa ggaccagaaa tccttatagg
     6181 aactgctgga cgcgtatttg aacttgtgaa actaaaaaaa ataaaaatga tgaacattaa
     6241 tactatcgtc cttgatgagt tcgatgaatt actcggcgac tcacaatatc attttgtgga
     6301 caatattatt aatcgcgttc ctcgtgatca gcaaatgatt tacattagtg ccaccaacaa
     6361 actggacaat agtaaacttg ccgataacac tataacaatt gacctatctg atcaaaaact
     6421 tgatactata aaacattatt atattacagt tgataaaaga gagcgtactg acttacttcg
     6481 taaattttca aatattcctg attttagagg tcttgtcttt tttaacagct tatcagactt
     6541 aggtgcatgt gaagaacgtt tacaatttaa tagggcctcg gcagtttctt tagcaagcga
     6601 tattaatatt aaattccgta aagttatctt agagaaattt aaaaaccatg atatttcatt
     6661 actcctagga actgatttgg tagctcgtgg cattgatatc gacaacctag aatatgtgat
     6721 taattttgat atcgcacgcg acaaagaaac atatacacat cgttctggac gaactggtcg
     6781 tatggggaaa gagggatgtg ttattacatt tgtaacacat aaagaagagc tcaaacaact
     6841 taaaaaatac gccaccgttg cagaacttgt acttcacaat caaaaattac acttaaaata
     6901 aaaaagaaac aagtaacttg tttctttttt tattaaggcg ttgaatataa ttcacctaca
     6961 gtaacaaaat aataaccctg ggttgtcaga taatccataa ttgtaggcaa agcatctatg
     7021 gatgtctggt gaatatcatg catcaaaatc actcccccag gacgtaattg ttttttgata
     7081 ttggtcatca ttgcttgtgt attatgattt ttccagtctt ctgtatcaac tgaccacaac
     7141 atttctttca atcctgtcac tgtttttaca gtagcatttg ttgctccgta agggggacgt
     7201 aagtacactg gtttttgtcc tgttgctttc atgattagat cattagtctt atgaatctct
     7261 tccttgattt tctccgctgg tagtttggtt aaattaggat gatcccatgt atgattacca
     7321 atctcatgac cttcagcatg catcctttgt aaaatatctg tgtgccctac agcactttgt
     7381 cccattataa agaaagtcgc tttagcatta tatcttttaa ggatttctaa ggctttagga
     7441 gtcgtttttt catttggtcc atcatcaaag gtaagtgcaa cacgtggtgc tttagctttt
     7501 aagttagaaa ctaaatctag atcacttgat tttaaatatg actcatcgat cacattatat
     7561 aatttatcaa gtggtaattt taaatcatta ataattaact taccatcttt aacatctagc
     7621 ccatctgtta ctcttccagt ttttaaaaat tctttattgc tatcatgttc ctttagttga
     7681 gttcctggag ctatattttc taaaatagcc ttaaatccag atatattatg agctaaaagc
     7741 tcatctaatt caaaaggttt cttattttga tcaagtaagt aggttctcaa aaggcgactt
     7801 gatttttgaa acatacttcc tttttctttt tgatataaga tttgataaat ggataatttt
     7861 ttaacatttg caaagttaga atctgctaat tgaggacgta gaataatgta ttgattgtca
     7921 taaaattttt tatgctcaaa acttttttga ttccacttta aaatatcagc gcttagactt
     7981 ttgtcatatc ctttaggttc aagaaaaaga acacgttcag ttttctgtct tttcaccact
     8041 tctaccgttt tctcggctat gcgaatttct ttttcaacaa cagcttgttg aagttttatt
     8101 tccttatgtt tcaaaaacga aaaaccttgc caagcaagaa tcgctataat aattagacta
     8161 aaaataccta taattaattt cttcatataa tgactctttt gttatttttt atgacaatta
     8221 tatcacaaaa ataaaaccac tttagaagat tatataattc tttattttta atgtaaaaga
     8281 ggtttataaa acgactcaac tgacaatcgt tgagtcgttt tacatctatt tcatatcaaa
     8341 aacaatcgat tttacatttg tcattgcttc aattgaatat ctgatacctt gaacacctgc
     8401 accagatcct ttgagtccta agaatgggaa gttatcagga ccacgtccag tcttattgtt
     8461 aatgtgaact gtaccaactt caagtttatt tgctatatca aatgcttttt ggaaatcacg
     8521 tgtaaagact gatgattgta atccaaaatc agatttgttg gcaatagcaa cagcttcttc
     8581 tgcatccttg acacgaataa ttgggagaat tggtccaaaa ggctcttccc aagctagttt
     8641 catatctaaa gtaacatgat caaacaatcc tggagttaat agacgaccat cacgtttaaa
     8701 ttcattcaat tctttcgcac ctttttgacg tgcatctact actaagcttt caataaagtc
     8761 agctgaatta tcatcaataa ccggtgtcac cgttgcatta tcaaatggat cacctactga
     8821 taattttgct acattttcag atattttttc tgccaattca tctgcaactt cttcaacgac
     8881 aagcacacgc ttaattgccg tacagcgttg tccagagtaa tcataagcac ctgcaacgat
     8941 ttgtttagca gcgttatcaa ggtcagcatc agctaagacg atacctgcat ccttaccgcc
     9001 caactcaagc ataattggac gcattcctgc caacttacca atacgttgtc caactggcgt
     9061 tgatcctgta aagttaataa aattaacttc ttcatgctca acaatgtaat ctccaatctc
     9121 agaaccgcgt cctgtaatag tattaaagac acctgctgga agacctgctt ctgcaaaagc
     9181 ttttgctaaa acaagtcctg agactgaacc ttgtgttggt ggtttaaaca tcacaacgtt
     9241 tccaccaatt agagctggcg caatttttga tcctgagagg ttaacagggt aattataagg
     9301 tgcgattgct aaaacgatac ctactggttg acgacgaata acagccaact tatgacctgt
     9361 tgaagcatcc atttttccac cttcgtcagc tgaagttgat aaacgaattc cttcttctgc
     9421 tgcataacga ataagatcag ctgtccttac aacctcagtt actgaagcat tgtaagcttt
     9481 agaaatttct tttgctaaaa cagtagcaat ttcttcttta tcacgttcaa taatgtctgc
     9541 ggctttatga aggtattgtg cacgttcata tactgttaaa gcagcccaag ctggtaaagc
     9601 ctcacgaccc gctttcatag catgatcaac ttcttctcga gtcatcgctg gcacgaatcc
     9661 caatgaagaa tcatcaattg gtgacaaaat ctcaatctga ttaacagatg atttccattc
     9721 gccattgaca taattttgat attcttttgt caaagtaaat ctccttttac attgtaatag
     9781 gtttattcta tcactttatc tatttatttc aattatctga catatttttc ttagaaactt
     9841 aacaatatcc aaactttcaa taaaaaagta gagatttttt aatccctact tttttattat
     9901 tctcagtcaa agtcaacgta ttctttttga agttcgataa cttcttccat agttgaacac
     9961 tctgaaagag cacgattagc gtattcttcc attttagctg tgtccaattt tttcattaag
    10021 ctacgtgtac gaagtactga ggttgctgac attgagaatt catccaatcc cataccaaca
    10081 agaagtggaa ccgcagtttg atcaccggcc atctcaccac acatacctgc ccatttacct
    10141 tcagcatgag cagctttgat aacgttgtta atcaaacgaa ggattgatgg attgtatggt
    10201 tgatagaggt atgaaacttg ctcattcata cggtccgcag ccattgtgta ttggataaga
    10261 tcattagtac caattgagaa gaaatcaact tcttttgcaa attggtctgc aagcatagct
    10321 gccgctggga tttcaatcat aataccaact tcaatgcctt cagctactgc tacaccgtca
    10381 gctaacaagt tcgctttctc ttcttcaaat atagctttag cagcacggaa ttctttaagc
    10441 aaagcaacca ttgggaacat gatgcgtagc tgtccatgaa ctgaagcacg aagaagtgct
    10501 cggatttgtg tgcggaacat tgcatcacca gtttcagaaa ttgaaatacg caatgcacgg
    10561 aatcctaaga atgggttcat ctcttttgga agatcaaagt aaggaagctc tttatctcca
    10621 ccgatatcca ttgtacgaac aacaactggt ttaccattca taccctcaag aactgcttta
    10681 tatgcttcat attgctcatc ttctgttggg aaatcttgtg aatccatata taagaactct
    10741 gtacggtaaa gaccaactgc ttctgctcca ttttcgttaa caccctcaac atctttaggt
    10801 gtaccgatat tagcagctaa ttcaaaatgt ttaccatcag cagtaactgt ttgtgcatcc
    10861 ttaagaagtg cccattcagc tttttgcttg gcataagctt cacctgctgc cttgaaggct
    10921 gaaatttgag cttcagttgg ttcaatgata acttcaccag taataccatt aacagcaatc
    10981 aactgaccat cttgaacacg ttcagtaata tcatttgtac caagaacagc tgcgatttcg
    11041 agtgtacgtg ccataatagc tgagtgactt gtacgccctc caatatttgt tacaaaagct
    11101 ttaacaaatt gtttatttaa ttgagctgta tcagaaggtg tcaaatcgtg tgcaataacg
    11161 attgattctt cattgatagt tgccgggttt ggtaatttaa cacccaaaag atgtgctaag
    11221 acacgtttgg caacatcacg gatatctgct gcacgttctt gcatgtatgg gttatcttcc
    11281 ataccttcaa agatagtgat gaacatatca gtcacttctt taagacctgt ttctgcattt
    11341 acttgttttg cacgaatagt ctctttaatt tgattaatca tttctgggtc agaaagaacc
    11401 attaaatgag catcaaatac agctgcggct tcttcgccta agctttctac tgccttctca
    11461 cggataacag aaagctcgtc ttgtgaagct tggagtgcaa catcaaggcg agcttcttct
    11521 gcgtttgtat cttcgactgt tacagtctca aatgacaaat ccggttggac aagtagatat
    11581 gctttagcaa ctgcaacacc atcagaggct gcgattcctt taagcatttc tgtcattttc
    11641 ttatgccaat ccttcttttg tcattgtttc ttcgatagct gcaattgcat catctgcatc
    11701 tgcaccttct gctgaaatag taacatcagc accttgacca acaccaagac tcataacacc
    11761 catgattgat ttaaggttta ctgctttacc tttgtaatca agcgtgatgt ctgaagcaaa
    11821 tttgctagct gtttggacaa gcaaagtagc tggacgtgcg tgaatacctg tttctgcaac
    11881 aatgtggaaa tcttttgaag ccatattatg gttctccttt tgtcatttgt aaatatttga
    11941 gtcacctatt ggtaaccctt acaagttaaa agtataacat attgcttttt atttttcaag
    12001 attaaatgtc tcaaacagaa aaaaacttga atgttttcca cagcttttca tattaaaaat
    12061 gagagtctta gaatttaaaa atagaattct aaaaatgatt tttttatgtt cttctgaaag
    12121 gaattggaaa acaggctgaa agcttttaaa ctaaccctaa aaacgaaata tagtgtctca
    12181 ttttatttta agacactata tattgtgttt tgttagaaat aaaacacttg ctttttccat
    12241 tcttttttta tatgctatta ccatgaaaaa aaattaggag gacaacatgg cagctattac
    12301 tgttttttcg aaaaacaact gtatgcaatg taaaatgact aagaaatttc ttgaccagca
    12361 cggcgctgat tttgaagaaa ttaatattga tgaaaaacca gaaaaaattg aatatgtgaa
    12421 aaatctcggc ttttctgcgg ctccagtgat tgaagctggt aatgttgttt tttcaggttt
    12481 ccaaccaagc aaactaaaag aacttgttta agcaaattag agtctgggaa taaagcaatt
    12541 aattgcttta ttcccatttt gtattgttaa gttaagagga taaattcaat gagtttaaaa
    12601 aatattggcg atgtatctta ctttcgccta aataatgaaa ttaaccgtcc tgttaatgga
    12661 caaatcccac tccataaaga taaagaagcc ttaactgctt ttttcaaaga aaacgtacag
    12721 cctaattcaa aagcttttga ctcaatcact gataaaattg cttacctcct taagtatgat
    12781 tatcttgaag aagctttcct caataaatat cgtcctgaat ttattgaaga attatctacc
    12841 aaactcttcg ataaaaagtt tcgttttaaa tccttcatgg cagcctataa gttttatcag
    12901 cagtatgcat taaaaacaaa tgatggagaa tactatttag aaagtattga agatcgcgtt
    12961 ctctttaacg ctctttattt tgcagacggt gatgaagaac tagcaactga cttagctcta
    13021 gaaatgattt cacaacgcta tcaacctgca acaccaagtt ttttaaatgc tggtcgaagt
    13081 cgtcgcggag aattagtctc atgtttctta atccaagtaa ctgatgatat gaatgctatt
    13141 ggtcgttcga ttaattcagc tcttcaatta tctcgtattg gtggtggggt tggaatatca
    13201 cttagtaacc ttcgtgaggc tggagctcct atcaaaggtt ttgctggtgc tgcttctgga
    13261 gtcgttccag tcatgaaatt atttgaagac agtttttctt actccaacca acttggacaa
    13321 cgccaaggtg ctggagttgt ttaccttgac gttttccatc cagatattat ttcattccta
    13381 tcaactaaaa aagaaaatgc tgatgaaaaa gtacgtgtta aaacattgtc attagggatt
    13441 actgtacccg ataaattcta tgaactagca cgtaacaatc aagaaatgta cttgtttagt
    13501 ccttatagta tcgaacgtga atatggtgtt cctttcagct atattgatat taccgaaaaa
    13561 tatgatgaat tagtcgctaa ccctaatatc actaaaacaa aaatcaatgc tcgtgattta
    13621 gaaacagaga tttctaaact tcaacaagaa tctggttatc cttacatcat caatattgac
    13681 actgctaatc gtactaatcc tgttgacgga aaaattatta tgtcaaatct ttgctctgag
    13741 attttgcaag tacaaaaacc aagtcttatc aatgatgctc aagaatacct tgaaatggga
    13801 actgatattt cttgtaacct tggctcaact aatgttttaa atatgatgac atctcctgat
    13861 tttggaaaat caattaaaac aatgactcgt gctctaactt tcgttactga ctcatcaaat
    13921 attgaagctg ttccaactat taagaacgga aatgctcaag cacatacatt tggacttgga
    13981 gcaatgggac tccactccta tttggcaaaa aatcatatcg aatatggtag tccagaatca
    14041 attgaattta ctgatattta tttcatgcta atgaactact ggaccttagt agaatcaaac
    14101 aatattgccc gcgaacgtca aacaacattt gtaggttttg aaaaatcaaa atacgctgac
    14161 ggtacatact ttgataaata tgtatctgga aaatttgttc cacaatctga caaagtaaaa
    14221 tccctattta ctaatcattt tatccccgaa gctaaagatt gggaaaacct tagatacgca
    14281 gttatgaaag acggactata tcatcaaaat cggcttgcag ttgctccgaa tggttctatt
    14341 tcgtatatca acgattgttc agcatctatc caccctatta cacaacgtat tgaagagcgt
    14401 caagagaaaa aaatcgggaa aatttattat ccagctaatg gcttagctac agatactatc
    14461 ccttattaca cttctgccta tgatatggat atgcgtaaag taatagacgt ctatgccgca
    14521 gcaactgagc acgtagacca aggtctctca atgacactct ttttacgaag cgaattacca
    14581 aaggagttgt acgagtggaa aacagaaagt aagcagacta ctcgcgacct ttcaatccta
    14641 cgtaactatg ctttcaacaa aggtgtgaaa tcaatctact atatccgtac ttttacagat
    14701 gatagtagcg aagtcggagc taatcaatgc gaaagttgtg tcatctaacg aagaaactct
    14761 acttaaaaca tcagaaacta gactgagata aactatctca gtttcatgat gttatcttat
    14821 tttctaaggt attttcatct ttatttttct aaaaatcgat aatacttgaa atattgctag
    14881 atcagaattc tatattctac tcttcttcca tcatcattat ataaaaacct atttaccatt
    14941 ggagaaaacc atgacaactt actacgaagc tataaactgg aacgaaattg aagatgttat
    15001 tgataaatca acttgggaaa aactaaccga acaattttgg ctcgatacac gtatcccttt
    15061 atcaaatgac ttagacgatt ggcgcaaact ttccgctcaa gaaaaagatc ttgttggcaa
    15121 ggtttttgga ggcttaaccc tacttgatac catgcaatca gaaactggtg ttgaagctat
    15181 tcgtgccgat gttcgcacgc ctcacgaaga agctgtctta aacaatattc aattcatgga
    15241 atctgttcac gctaaatctt attcttcaat tttctcaact ttaaatacta aatcagaaat
    15301 tgaagaaatt ttcgagtgga ctaataataa tgagttcctt caagaaaaag cacgtattat
    15361 caatgacatt tatgctaatg gaaatgccct tcaaaaaaag gtggcttcca cctacctcga
    15421 aactttcctt ttttattctg gctttttcac acctctttac tatttgggaa ataataagtt
    15481 agcaaatgtt gctgaaatca ttaaattaat tattcgtgat gaatctgtac atggtactta
    15541 tatcggttac aaattccagc ttggttttaa cgaattacca gaagatgagc aagagaattt
    15601 tcgtgattgg atgtatgacc tcctttatca gctgtatgaa aacgaagaaa aatacaccaa
    15661 gacactttat gatggcgtag gatggactga agaagttatg acctttttac gctacaatgc
    15721 taataaagct cttatgaatt taggacaaga tcctttattc ccagatacag caaatgatgt
    15781 caacccaatt gttatgaatg gtatttcaac aggaacatca aaccatgact tcttctctca
    15841 agtaggtaat ggttacctac ttggtagcgt tgaagctatg catgatgatg actataacta
    15901 tggattataa gaaaaagaca ctgatttagt gtctttttct agttaaatac aaatttcttg
    15961 acaagcaaac cctttcaaga tagaatgtta gagtaaatac acaagggagt gccttgagct
    16021 gagattgcag atatgcaaaa tcctctaacc tgatctcgtt aggacgagcg taggaattgt
    16081 ggtttgaatt ggttaaacat gacaaatgac aaaagaatct cctttgctgt ataaaaaagg
    16141 agatttttta tgtcaaaaaa taacaacact acctgtctta ttgaaactgc tatttttgca
    16201 gcgcttgcta tggctttatc aatgattcct gattttgcgt catggttcac tccctctttt
    16261 ggagcaattc ctctgatact ttttgcatta cgtcgtggta caaaatacgg tctatttgct
    16321 ggcttaattt ggggcttatt gcactttgtt ctttccaaag tatattacct tagtttatcc
    16381 caagttttta tagaatacat cttagcattt ataagtatgg gacttgcagg cgtattttct
    16441 gctaaattta aagatgcact tagcagcagc tctaaaacta aagctttatc attagcatta
    16501 tctggagcaa tattagcaac atttgttcgt tatgtttggc attatatcgc tggtgttatt
    16561 ttttgggcta gttatgcacc aaaaggtatg tctgctacat tatactcatt aagcgtcaat
    16621 ggtactgctg gtttgttaac tctatttttt gtagtcatta gtattattat tttagttatc
    16681 agctacccta gcttcttttt acctaagaaa tagaaaaaat aaaaggactg attttcagag
    16741 aatcaatcct tttattttac actaatataa gttttttgat aaaaaatcta gctactacta
//
LOCUS       DATRSG010000049        16676 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRSG010000049 DATRSG010000000
VERSION     DATRSG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.049"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..138
                     /locus_tag="VBD89_001472"
     CDS             <1..138
                     /locus_tag="VBD89_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135990.1"
                     /translation="RDVVVTYDLKENGTLKYDTGSYFTSGNENIAYKKDSEAYKKALK
                     K"
     gene            complement(158..1009)
                     /locus_tag="VBD89_001473"
     CDS             complement(158..1009)
                     /locus_tag="VBD89_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO7135991.1"
                     /translation="MQRIYFNVWNRVKQTLYPFMKLNKVHPLYYTFQDYFNYEVDKKN
                     ILVIPHHIRIDIAGFNINDDVLTISYQDSDSITRQNFTKCHELGHILLHHSGRVFTDF
                     HDDSLQEREANFYSAFLLMPDIVLLTKIFYQQMAFQEVEAALQVSGQALKTRLTDFLN
                     FELDINRLSAKQIVEDYLTRKNKHIVGYFDQVKDYIISDYNEFEPSALDKFNYLINQS
                     NFISDIDLPELADTQFTSLIKQCYPKFKIWGLYDKGQSISYAWNPKQVTEVEAKRHAK
                     LLLITRK"
     gene            complement(1016..1378)
                     /locus_tag="VBD89_001474"
     CDS             complement(1016..1378)
                     /locus_tag="VBD89_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7135992.1"
                     /translation="MFPERLKALRLEANLTQSDVAKEFGISQQAYAKWENAKANPTQS
                     VITKLANFYNVSIDYLMGKSDYKNSDEIDLSTFEVLYRKTSKNLSDEEKKILEEDLRE
                     FLLEREKAIRAMNKEKKD"
     gene            2059..2331
                     /locus_tag="VBD89_001475"
     CDS             2059..2331
                     /locus_tag="VBD89_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO7135993.1"
                     /translation="MATVPTQIRIDETVKAQATSLFNDLGMDMSSAVNIFLRQCLLRG
                     GLPFTVEVPNYSQKTLEALAEAKRISRDKTIASYDNLADLKKALDA"
     gene            2332..2610
                     /locus_tag="VBD89_001476"
     CDS             2332..2610
                     /locus_tag="VBD89_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEO7135994.1"
                     /translation="MYQVKFTSTFKKSYKRIKKRGLEMTLLDEVIEKLRLGQTLEEKY
                     RDHELKGNFVGFRECHIKPDWLLIYLIEDDILTLTLADTGSHADLFKM"
     gene            2739..2975
                     /locus_tag="VBD89_001477"
     CDS             2739..2975
                     /locus_tag="VBD89_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135995.1"
                     /translation="MNDYEQSFRRIYENFVFSLKIYPDKHYQKVLCYQVLEKMDKLFH
                     DYQKLGLPTVQLVQWKNHYKFKVQHDYIMKGGTT"
     gene            2972..3199
                     /locus_tag="VBD89_001478"
     CDS             2972..3199
                     /locus_tag="VBD89_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEO7135996.1"
                     /translation="MNYFEQRFQQIYEKFLFSLKIYHANPTHCETCYRDCLNEMDSLF
                     LRHDTHDQFAKQLLNCKKTFQFKVKKAYFGM"
     gene            3313..3627
                     /locus_tag="VBD89_001479"
     CDS             3313..3627
                     /locus_tag="VBD89_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7135997.1"
                     /translation="MEITKVFVDQNQVLGNEVFVSGMEEVRANDYNPVSYRIFEISSR
                     KEPSAFLIVEGFKMSDVPEGRQKVVFPRGIAIERRNFRFGGKRKHENVIVAEEMRTVK
                     SK"
     gene            3650..4060
                     /locus_tag="VBD89_001480"
     CDS             3650..4060
                     /locus_tag="VBD89_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HEO7135998.1"
                     /translation="MTVKFAADVIETFDTVKTLGTLNFLEAIPFMHWEDYVEEGTGEE
                     KRRETDKVDYVDVKLYSSAVNGDITVTVPPEAKVAQMTAEKNYNDEVTLVAPTARFWS
                     NAETINNRRITTSGVKLRAKDVVLVGKPEPKKEG"
     gene            4067..5725
                     /locus_tag="VBD89_001481"
     CDS             4067..5725
                     /locus_tag="VBD89_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO7135999.1"
                     /translation="MKLFTYRGIRVRKFHEKIKQLTFALFLLPFLLGGGILAYLHQNQ
                     LQNQFQRDPLTYGILFGGSVLGLLLLDGLVQVICYRNFLFFSRLDSLRILSHFLLDNN
                     LYKVNKIKSNHHTRERILLPKVYLKRDHFSITVSLHLQGSRFQDRFIALEKPLEIMFD
                     GDFMNKQFTKGYVSYTIAIDQFSGRLSIFDVKMTDKGIRLMKDVYWDFNKHPHLLIGG
                     GTGGGKTVLLMILIWVLAQIGYVEILDPKRSDFVGLKNIPVFKGRVYWDKKEMLACLK
                     RAEQEMDKRYDYMTSHPDYQAGKKYYAYGLKPRFIVIDELAALAAKLERDYQSSGDFI
                     EYLTELILKGRQSGVIMIVAMQRPDGEYLKTSLRDQFMKRISVGHLEDTGNKMMFGDA
                     NANKVFKKIDEIDGEEIFGRGYIANGGEVAREFYSPTVPFEEGFSFFEEYKKLPVLDD
                     PLLETTETTQTSSSDLSVEQAESPLLPLNDFAKEFDVPISTLRNLVKYIEEQDYTFRR
                     EDNRILLDEADRALLEEIVTEKEQADLPYKQVVLQHFETPPEPA"
     gene            5937..7169
                     /locus_tag="VBD89_001482"
     CDS             5937..7169
                     /locus_tag="VBD89_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7136000.1"
                     /translation="MISNTDLKRIRLELGFTQRKMATMIGYSYYNYRNIEQGQRKMTK
                     EFEEALFHFLNRKSETKLESTVDWLKIRFKTLDFKAVITHVLKLKLADFFYEEKSLYS
                     YSDMITYGSIRVLYSHSEKKAEAGTLIDLTGGGCRELELLLKQQGRNWFSFLHDVFLF
                     AEQERKDRPLEVFLAFPRFDIALDELYKKSGNLDLFDIKNRIFDNKIIMRKLRTFTAI
                     EGLKKVENRFVNQGLTLNFGSRQSNLMIRFYQKDYEQALLKDVSVDYIHEAYNYKNRY
                     EIELHDTKAFDILKEWYTLETDLTKIGARILNNYFEVKDWNGNYDSEWDNLLGTQAGF
                     KFVTRPRQINYARTKHWVTKQVSSALKLLKIVDMVYQTDELSEIISEAYLSNNHTKLA
                     EEICERNGVDFNVIVGQI"
     gene            7147..7533
                     /locus_tag="VBD89_001483"
     CDS             7147..7533
                     /locus_tag="VBD89_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136001.1"
                     /translation="MSLSDRYKPFNVPDKFNRPLQTKTFPVGYEELYLSFYDFELVKD
                     LIDYWGLLYYQPKKDSELKYAEQFRKQAFKDENHQQNAIKKATRQEARQPFFEELKTK
                     PLKKMSQNARWVAEMLVQTGYAQLVL"
     gene            7553..7789
                     /locus_tag="VBD89_001484"
     CDS             7553..7789
                     /locus_tag="VBD89_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002270998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136002.1"
                     /translation="MEYKVELNSLDNFRAWSGARNTLATVRERGDMDRLTSLGEDIFS
                     GSIPTETEINDWLWFDSDDIYRFLGYHDLVEDDV"
     gene            7786..8340
                     /locus_tag="VBD89_001485"
     CDS             7786..8340
                     /locus_tag="VBD89_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7136003.1"
                     /translation="MKLVKKIYEGISCFLDKNEFWNLYIVLMKEKEFFLDAFARKTID
                     LEYPAHYQHAYFTLDGQVLDFNQHMTTQLVTLFRQVILENQITFMEELIMATQNTLEK
                     KVRAVSLELGELMKAHDDKEAWKKAGELHGLLKKEEVKQLPEALVESLHAELRGYYYV
                     NSELNKLHKQLYAKGNKLIELANQ"
     gene            8350..9342
                     /locus_tag="VBD89_001486"
     CDS             8350..9342
                     /locus_tag="VBD89_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7136004.1"
                     /translation="MNTVKLIYHQLKTYLGNFTKRPKKKGKPTLTLANKKTVNLAVLS
                     GLAFILLVGLLGGIRAMTMSSKVTNLEKLIVAKKATSSPSSAYQTIDNRLQYYLNDFV
                     ECYFTIPADSDGQTKQAKQLTSFYGSEPDIQAQGQVKNPSQLSWSRLLTVTDNVATYE
                     VHYNQTVKNGDKTEEKELVTGFNIPYAKTKTGYYVTGLPWFSSLSTNQADKKSTDTEV
                     KLNQSDRVSEKTKKKLDKFLNVFFTNYTTDQDNLDLVAHNVTVVKNATFKTLDFSYYK
                     ATDKAVMAYVQVTFEVAGSTHAENFTLTLSEKGKSYYVEKVSHTIPANYADTKE"
     gene            9359..9586
                     /locus_tag="VBD89_001487"
     CDS             9359..9586
                     /locus_tag="VBD89_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136005.1"
                     /translation="MSSSNLQTWLQGDGFWFITFGAIILIILAWRNSSWGRLFTTLVF
                     YAVIASLTGGGRQLLHVIGWFLRLFGIETGL"
     gene            9588..10004
                     /locus_tag="VBD89_001488"
     CDS             9588..10004
                     /locus_tag="VBD89_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7136006.1"
                     /translation="MEKERLYDYARGINAPYWIQEVKTQKGKRIWYFATPMQLSFFVV
                     FALVLILMLTVFSSLLLPLNKLTHSISCYLYWYIPYKLAKFYTEYEPQGKKMHVFLWD
                     YICYLMDFRLNRKAIYHEDRMKMVDEEEIVFEKTDL"
     gene            10015..12537
                     /locus_tag="VBD89_001489"
     CDS             10015..12537
                     /locus_tag="VBD89_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7136007.1"
                     /translation="MVLKLVYPILKTQDNIALTTDKMVMSFYRIPNTPITITDKAKKQ
                     KHKLTTAQLIKKLVKNKSFEVSLIPKDYLLEEKMKDFSHALSPDSQALGEELLQYSTD
                     KLTQEMEIPYQFDWLIGVNLLKDDQNTTVQDMAVERFSEVSEMIANSVGFQYEIPEDW
                     AQEYLVSEATVYQILSALRAQRLTNEELFYYQRMQYLRYIPHLKQEVTANRALFNVTD
                     TVLRPMAGGFMKLDSPYGSSFLTILPIAKTPIIFNGFHLGEFTQRFNFPVELRIKAEF
                     IEKNSLKGRMARSNIRYRNIMNEAESTDTVQQDEIILGAMSLKDLMKKVGGKEEVIEY
                     GAYLIVSASSLSQLRRRRQVVLSYFEDMHVEVSEASHDTPYLFQSLLYGQPLENKTRT
                     WTHYVTPRGFAELMPFTNTASGNHIGWHIGRVDNWTGRWESIEKAQEASKNMVLFNAT
                     VGNKEGIAGKLTKNPHILITGATGEGKSFLAELIFLLTSLQDVKSLYVDPKRSIRKHF
                     EAIAHHPDFEKRFPLLAEHIKQINFVTLDSRVKSNHGALDPIVMLDKDDAVSVSKNML
                     NYLIFSNKRMKVTMKQMTAISRAINKVVAQRQTGEIVGLKHVLKELTNNPDSTIFEVG
                     DYLTSIVENSILELAFSDGNVEGLSYDKRVTVLEVADLSLPDSKDNQQDVTPDDKEIN
                     STALMFALGAFCTRFGELNRYEDTIEFFDEAWVLMKSAEGRAVIQSMRRVGRFFNNVL
                     CLITQSVHDAEGDDDTTGFGTLFAFREDNELPDILEHVGLTDNEENLEWIKNMISGQC
                     LYKDVYGNLNMISIYNIFDSIDPLLKPMKATVSSNLENKYAS"
     gene            12550..14481
                     /locus_tag="VBD89_001490"
     CDS             12550..14481
                     /locus_tag="VBD89_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7136008.1"
                     /translation="MQEAFEKLKGMDIFSLKSYMIKTGFGDFSGVWVMFNEIFVNFPF
                     FLLNLIVGFFSLMIRILENVRLYDTYKQYVFSGAQSIWRGFTGATSGVAQSSLVFLIL
                     LCLAFYLFYQLLMSKGNFSQRLLHVLLVLFLGFGWFGTVAGTSGGLYLLNTVDNVAST
                     TIHHISNVSVSYGKNQSLKIGSSVADSYIAETSYKAYLFVNTGQENGKYKDRQTGKEE
                     PFEDSKVLGSLDKSGKFKAVSTADRNKYLEIGNGASDDNEHNRWVSAIPDYIPVKFFY
                     ILFKMVEAIVIAIPIVLIQLLNVLAQALVLIMILLFPIALLVSFIPKMQDILFGVFKV
                     MFGGLAFPAITSLLTLALLYLEKIIENLVSSGFDKVIGDFSSLSTFSALFELIVSVCA
                     KGVIYWLLWKYKGELIELLLGSRAKITFDEIDTKVKETVTNGQEPLKQLPTKATNAFE
                     MAQTSGNFFLAGAGLGAGLFMNAGKQMKTLFSNPHPTSEGTEDSTPPENDEEETPTTS
                     SFEETGAPDEVTTPFQEPDMPMEIFESAIQEEDTSVQPFDTEPSPLMDTKQNREQKEF
                     EERRQNRLSWRQKHHINKLEKELEPYKDDEALYQAQGSNAFIKNYRKTLPKDDKLKAN
                     INRKHQIIEELARLRGGKE"
     gene            14522..14704
                     /locus_tag="VBD89_001491"
     CDS             14522..14704
                     /locus_tag="VBD89_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136009.1"
                     /translation="MNNAVALAYKKRKPSIDFLLLFSVKEIEKEQLLATIKENPLILT
                     AQWRFETVMMTVYVKT"
     gene            14713..15768
                     /locus_tag="VBD89_001492"
     CDS             14713..15768
                     /locus_tag="VBD89_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7136010.1"
                     /translation="MKKKLVIVSALGALVLPLFLVVIIMGAFAGSTSSNGQAKGLQTK
                     LTAKEVAQKANISEERASDVIKILNYELSQEQFTLAGASGSLAVAERESGFDPKAVNT
                     GGGVAGYFQWSGWSSTVNGNRWSKAKQKKLDSDIELDLLSTELNGSYSKVKTEMQKAT
                     DPEEAALYWSEYYEGVALSDGQTKTSDLKKNARKWYNLFKDTLTGDTTNAKTRDGGGV
                     TSDGVPAGYSLTKAINTSNYTSATYPWGQCTWFVYNRAKEVGVNFDPYMGNGGDWKQK
                     AGYQTTHTPTEHSALSFSPEQAGADPTYGHIAFVEQVKSDGSILISETNTKGLGVVSY
                     RTFDKATANQFTYVIGK"
     gene            15825..16130
                     /locus_tag="VBD89_001493"
     CDS             15825..16130
                     /locus_tag="VBD89_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HEO7136011.1"
                     /translation="MTKKMFQKRVDSELLDEVSVLYKSLGTSVGDAFVMFLQKSKEVN
                     GLPFDLRKQVHAISDNPLERLALANSNVIKVTASNPEAIADFFDEDYPEYEERHGQL"
     gene            16117..16449
                     /locus_tag="VBD89_001494"
     CDS             16117..16449
                     /locus_tag="VBD89_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEO7136012.1"
                     /translation="MDNYNPYDILVATIAFSDGTGAKRRPVLVIANNEEVIRTYRITS
                     QYSRKSSYIRSKYFEIKEWEEAGLVKPSWIDTVQLYDLDVELVQVRLIGKLTENDQQR
                     FEKFLREN"
     gene            16560..>16676
                     /locus_tag="VBD89_001495"
     CDS             16560..>16676
                     /locus_tag="VBD89_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7136013.1"
                     /translation="MQVILPEEQTKEIQLFVANLIKNEIETVKEDSGQISPFL"
BASE COUNT         5451 a         3028 c         3278 g         4919 t
ORIGIN      
        1 cgtgacgttg ttgttaccta cgatttaaaa gaaaatggca ctttaaagta tgatacaggt
       61 agttatttta cttcaggtaa tgaaaatatt gcatacaaaa aggatagtga agcctataaa
      121 aaggcactga aaaaatgact tagagatttt ctctaggtca ttttcttgtt attaaaagaa
      181 gttttgcatg tcgtttagct tctacttctg tcacttgttt gggattccaa gcatagctaa
      241 tagattgccc cttatcatac aatccccaga ttttgaactt tggataacat tgttttatca
      301 aagaggtgaa ttgagtatct gctaactctg gtaagtcaat atctgaaatg aagtttgatt
      361 gatttattaa atagttgaac ttatctaatg cgctaggttc aaattcattg taatcagaga
      421 tgatatagtc tttcacttgg tcaaagtagc cgacaatgtg tttgtttttt ctagttaggt
      481 aatcctctac aatctgtttt gcggatagtc tattgatgtc taattcaaaa tttaagaagt
      541 ctgtcaaacg agttttgaga gcttgaccag aaacttgtag tgctgcttct acctcttgaa
      601 aagccatttg ctggtaaaaa atcttagtta gaagcacaat atcaggcatt aagagaaaag
      661 ctgagtaaaa attagcctct ctttcttgta gagaatcatc atgaaaatca gtgaatactc
      721 gcccactgtg atgtaataag atgtgcccta attcatgaca ttttgtaaaa ttttgacgag
      781 taatagaatc actatcttga taggaaatcg ttaaaacatc atcattgata ttaaaacctg
      841 ctatatctat ccggatatgg tgaggaataa caagtatatt tttcttgtca acttcataat
      901 taaagtaatc ctgaaaagta taataaagag gatgtacttt atttaatttc atgaatggat
      961 aaagtgtttg tttgacacga ttccaaacat taaagtaaat tctttgcacg aaacatcagt
     1021 ctttcttttc tttattcata gccctaatgg ctttttctct ctctaacaga aattctctca
     1081 aatcttcttc tagtattttt ttctcttcat cagataaatt tttagaagtt ttccgatata
     1141 gaacttcaaa tgttgataaa tcaatctcat ctgaattttt gtagtcagat ttacccatta
     1201 aataatcaat ggatacattg taaaaattcg ctaatttagt aataactgat tgtgttgggt
     1261 tagctttagc attttcccat ttagcatagg cttgctgaga aatcccaaat tcttttgcta
     1321 catcgctttg tgttagattg gcttctaaac gcagagcttt taagcgttca ggaaacattt
     1381 ttgtacctct ttaaaaaaaa ttaatttttt tacaaaaaac tcttgacaat acaacctata
     1441 agttgtatta tatatttaca acctgaaggt tgtgtaagtc ttgttaatac taaacctaaa
     1501 attatgaaat agataaattt aataaagtat gaaaaaggtc gatattaaca aaaagattta
     1561 aatttgtcct ttgaaaattg aataaaaaag tgcgtcagac aacctcactc aagcttagct
     1621 gaccaacaca caataatcat ttagcaatta ttattgtaca ggttttatcg caaaaagtca
     1681 aggaggttgg agtagatgaa caaaaaaatc ctttggagtt atctcacgca ctcggctttt
     1741 tgcccctctt gaccggataa caaaggggaa ctgctggcgc tggtggcgtc ggaaatgctg
     1801 acaggtaaga tacgttctgc tggggtaagc ataggcggtt aatcgtggct atagtgaagt
     1861 gagatgacta taataacaga gattagtcaa gtacagctat atagagcggt gataacaaca
     1921 ctgagtgtgc gacgatacga gcacgcccta aacagaggat tcatctgttt agacgagtag
     1981 aaaacactta aaaaaataca aatgattgac aatgtaaact atatggatta caataaataa
     2041 tgaaaggtgg tggcatttat ggcaactgta ccaactcaaa ttcggattga tgaaactgta
     2101 aaagcccaag caacttcatt atttaatgac ttgggaatgg atatgtctag tgcggtcaat
     2161 atttttcttc gtcaatgtct attgcgtggt ggacttcctt tcacagttga agttcctaat
     2221 tatagtcaaa aaaccttaga agctctggca gaagctaaac gaatctctcg tgataaaacg
     2281 atcgcttctt atgacaatct agcagattta aagaaggctc tggacgctta gatgtatcaa
     2341 gtcaagttca cctctacttt taaaaagagc tataaaagga ttaaaaagcg aggcttagag
     2401 atgacactct tagatgaagt gattgaaaaa ctccgtctag gtcagacact cgaagaaaaa
     2461 taccgtgacc atgaactcaa aggaaacttt gttggcttta gggaatgtca tattaaacca
     2521 gattggctct tgatttatct gattgaagac gatattttga ctttaacctt agccgacaca
     2581 ggcagtcatg cggatttgtt taagatgtga agacagttct ttgtgagctg tcttttttgt
     2641 tttctactcc tttaagctga tgaatcatta gcctatccat gtcctttcaa acggacagtt
     2701 caaaaaattg ccattctaaa ggaaagcgag gaaaaaagat gaatgattat gaacaaagtt
     2761 ttcgccgtat ttatgagaac tttgtttttt ctttgaaaat ctatccggat aagcattatc
     2821 aaaaggtctt atgttatcaa gtgctagaaa aaatggataa gttgtttcac gactaccaaa
     2881 aactaggctt gccaacagtt caattagtcc aatggaaaaa tcactacaaa tttaaagttc
     2941 aacatgatta cattatgaaa ggaggtacaa catgaattat tttgaacaac gattccaaca
     3001 aatctatgag aagtttcttt tctcccttaa aatttatcat gctaatccta cacattgtga
     3061 aacatgctac cgagactgtc tgaatgaaat ggatagtctt tttttacgcc atgatactca
     3121 tgaccagttt gctaaacagc ttttgaattg taaaaagact tttcaattta aagttaaaaa
     3181 agcctatttt ggaatgtaag gaggacggta tgaccagagt aagagcacct gccgattaaa
     3241 aagatgagtt gattttttga cctagacaag tcataaaacg gtctaaacaa aatagaaatt
     3301 gaggtaaaca taatggaaat tacaaaagta ttcgttgacc aaaaccaagt attaggaaat
     3361 gaagtctttg tatcaggtat ggaagaagtc cgtgccaatg attacaaccc tgtctcttat
     3421 cgtatctttg aaatttcaag tcgaaaagag ccctctgcct tcctcattgt agaaggtttt
     3481 aaaatgtctg atgtcccaga aggtcgtcaa aaagtcgtct tccctcgtgg aattgccatt
     3541 gaacgccgta acttccgctt tggaggcaaa cgtaaacatg aaaatgtcat tgttgctgaa
     3601 gaaatgcgta cagtaaagtc taaataagaa atagaatagg agaaacacta tgactgtaaa
     3661 atttgctgct gatgtgattg aaacttttga tactgtaaaa actcttggaa ctctaaattt
     3721 ccttgaagct attcccttta tgcactggga agactatgtg gaagaaggaa ctggtgaaga
     3781 aaagcgtcgt gaaacagata aggttgatta tgtagatgtg aaactctact cctctgcggt
     3841 caatggggat attaccgtaa ccgttccacc tgaagcaaaa gtggctcaaa tgaccgctga
     3901 aaagaactac aatgatgaag taacgcttgt agctcctact gctcgttttt ggtcaaatgc
     3961 agaaaccatt aacaatcgcc gtatcaccac aagcggggtt aaactacggg ctaaagacgt
     4021 tgtattagtg gggaaacctg aacctaaaaa ggaaggatag tagcctatga aactgttcac
     4081 ctatcgggga atccgtgttc ggaagtttca tgagaaaatc aaacaactaa cttttgctct
     4141 ttttcttctg ccctttctcc ttggaggggg tattttagct tacctgcacc aaaaccagct
     4201 tcaaaaccag tttcaacgtg acccgctaac ctacgggatt ctttttggtg gaagtgtatt
     4261 aggattattg ctactagacg gcttagtaca ggtgatttgt taccgaaatt ttcttttctt
     4321 tagccgtcta gatagtttac ggattctttc ccacttttta cttgataata acttgtataa
     4381 ggtcaataaa atcaaatcga atcatcacac gagagaacgg attctattac ccaaagtgta
     4441 tttgaagcgt gaccatttta gtattaccgt ttcactccac cttcaaggca gccgattcca
     4501 agaccgcttt attgcccttg aaaagcctct tgaaattatg tttgacggag acttcatgaa
     4561 caagcaattt accaaaggct acgtttctta taccattgcc attgaccaat tttctggtcg
     4621 cttatctatc tttgatgtta agatgacaga taagggaatc cgcctaatga aagatgtcta
     4681 ttgggatttc aataaacacc cacacttgct gattggtggt ggaacgggtg gcggtaaaac
     4741 ggttctcttg atgattttga tttgggtctt agctcaaatt ggttatgtgg aaattcttga
     4801 ccctaaacgc tctgactttg tcggattgaa aaatattcct gtctttaaag gtcgggttta
     4861 ctgggataaa aaagaaatgc tagcctgttt aaaacgtgct gagcaagaaa tggataaacg
     4921 ctatgattac atgaccagcc accctgatta tcaagcaggg aaaaagtatt atgcttacgg
     4981 gttaaaacct cgttttatcg tcattgatga gttggcagcc ttagcggcta aactagagcg
     5041 agattaccaa tcatctggag actttatcga atacctaaca gaattgattt taaaagggcg
     5101 ccaatccgga gtcatcatga ttgttgctat gcaacgtcct gacggggaat acctcaaaac
     5161 gtctttacgt gaccaattta tgaaacggat ttctgttggt cacttagaag atactggtaa
     5221 taaaatgatg tttggggacg ctaacgcaaa taaagttttc aaaaagattg atgaaattga
     5281 cggagaggag attttcggtc gtggttatat tgcaaacggt ggggaggtgg ctagagaatt
     5341 ttattcacct acagttccct ttgaggaagg cttttctttc tttgaggaat acaagaaact
     5401 acccgtctta gatgacccac tattagaaac aacagaaact actcaaacgt catcatctga
     5461 cttgtccgtt gaacaagcag aatctcccct ccttcctctg aatgactttg ccaaagaatt
     5521 tgatgtgccc atttcaacct tacgaaacct tgtgaaatac atagaagaac aggactatac
     5581 attcagaaga gaggataacc gtattctgct agatgaagca gatagggctt tgttggagga
     5641 aattgtaaca gagaaagaac aggctgactt gccctataag caagttgtcc ttcaacactt
     5701 tgaaacacct cctgagccag cgtaggcacg aaggtcaagg tcagggctac gcccgtccga
     5761 cctgcccgac cgagctgaca agcgggcgtt ttggggtgtg ccctaggcac gccccaaaag
     5821 ggcttaacct cccgatacta ataggggggt taaaactaac acaagtcctt aaaaggctgt
     5881 tagattccct attcttcccg tcactagaac aaacacaaaa aggaggtgga aaaagagtga
     5941 taagcaatac cgatttaaag cgtatcaggc ttgagttagg atttactcaa cgcaaaatgg
     6001 caactatgat tggttatagt tactacaact accgaaacat tgaacaaggt caacgaaaaa
     6061 tgaccaaaga gtttgaagaa gcactctttc actttctgaa tcgcaaatca gaaaccaagc
     6121 tagaaagcac ggtggattgg ttaaaaattc gcttcaaaac acttgatttt aaagctgtca
     6181 tcacacacgt tctaaaatta aaactagctg acttttttta tgaagaaaaa agtctataca
     6241 gctattcaga tatgataacc tatggttcaa ttcgggtact ttactcccat tctgaaaaga
     6301 aggcagaagc tggaacgctg attgacttaa caggaggggg ttgtcgtgag cttgaattgt
     6361 tattaaaaca acaaggtcgg aattggtttt cttttcttca cgatgtcttt ctctttgcgg
     6421 aacaagaacg aaaagataga cctttggaag tttttttagc ctttccacgc tttgatattg
     6481 cccttgatga attatacaaa aaatcaggga atcttgactt atttgatatt aaaaatcgga
     6541 tttttgacaa taaaatcatc atgcgcaaac tccgcacctt taccgctatt gaaggcttga
     6601 aaaaagttga aaatcgcttt gtcaatcaag gtttgacctt aaactttggc agtcgtcaat
     6661 ccaatctcat gattcgcttt tatcaaaagg attatgaaca agccctctta aaagacgttt
     6721 ctgttgacta tatccatgaa gcttataatt ataaaaaccg ttatgagatt gaactacatg
     6781 ataccaaggc gtttgatatt ttaaaagaat ggtatacgtt ggaaactgac ttgactaaaa
     6841 taggcgctcg gattttaaat aactattttg aagtgaaaga ttggaatggc aactatgata
     6901 gtgaatggga taacctgctt ggcacacaag cgggttttaa atttgtcaca cgtcctcgtc
     6961 aaattaacta tgcaaggaca aaacattggg ttacaaaaca agtctctagt gctttgaaat
     7021 tgctgaaaat cgttgatatg gtttatcaga cagatgagct ttcagaaatc atttctgaag
     7081 cctatttgag taacaatcat acgaaattag cagaagaaat ttgtgaacga aatggagttg
     7141 attttaatgt cattgtcgga cagatataaa ccattcaatg ttcccgataa attcaatcgc
     7201 cctcttcaaa caaagacctt cccagtcggt tatgaagaac tctacctatc gttttacgat
     7261 tttgaattgg tcaaagattt gattgattat tggggactct tatattatca acctaaaaaa
     7321 gattctgaat tgaaatatgc agaacaattt agaaagcaag cctttaaaga tgaaaaccac
     7381 caacaaaatg cgattaaaaa ggcgactagg caagaagcta gacaaccttt ttttgaagag
     7441 ttaaaaacaa aaccactaaa aaagatgagt caaaacgctc gttgggtggc agaaatgctc
     7501 gtacagacgg gctatgctca attggtacta taaaaatgaa aggaaaaatg aaatggaata
     7561 taaagttgaa ttgaatagtc tggacaattt cagggcttgg tcaggtgcac gcaatacact
     7621 agcaactgtc cgtgaacggg gagatatgga cagactaacc tcacttggtg aagatatttt
     7681 ttcaggaagt atccctacag aaacagaaat caatgattgg ctttggtttg attctgatga
     7741 catctatcga ttccttggct atcatgattt ggtagaagat gacgtatgaa gcttgtaaaa
     7801 aaaatttacg agggaatctc ctgtttcctt gataaaaacg aattctggaa tctctacatt
     7861 gtcctaatga aagaaaaaga gtttttctta gacgcttttg cacggaaaac cattgacctt
     7921 gaataccccg cccactacca acatgcttat ttcacactgg atggtcaagt gttagacttc
     7981 aatcaacaca tgacgacaca acttgtcact ctttttagac aggttatctt agaaaatcaa
     8041 ataacattta tggaggaact gataatggca acacaaaaca cacttgaaaa gaaagtcaga
     8101 gcggtctcat tggagctagg agaattgatg aaagcccatg atgataaaga agcttggaaa
     8161 aaagcaggtg aattgcacgg acttctcaaa aaagaagagg tgaaacaact ccctgaagca
     8221 ctggttgaaa gtcttcacgc tgaattgcga ggttactatt atgtcaatag tgaacttaat
     8281 aaacttcata aacaacttta tgcgaaagga aataaattga ttgaactagc caatcagtaa
     8341 ggggtaccta tgaatacagt aaaacttatc tatcatcaat taaaaactta tcttggaaat
     8401 tttaccaaac gtcctaaaaa gaaaggaaaa ccaacattaa cccttgccaa caaaaaaaca
     8461 gtcaatcttg ccgttctatc aggattggct tttattttgc ttgtagggct actaggaggc
     8521 attcgtgcga tgacaatgtc tagtaaagtc accaatcttg aaaaactgat agttgctaaa
     8581 aaagctactt cttctccctc ttctgcttac caaacaatag ataatcgcct gcaatattat
     8641 ctgaatgatt ttgtagagtg ctatttcacg attcctgcag attcagacgg tcaaaccaaa
     8701 caagctaagc aattaacgag cttttacggt tcagagcctg atattcaagc acaaggtcaa
     8761 gtcaaaaatc ctagtcaatt gagttggtca cgcctgctaa ccgttacaga taatgtggca
     8821 acgtatgagg tgcattacaa tcaaacggta aaaaatgggg ataagacaga agaaaaagaa
     8881 ttggtaacgg gttttaatat cccttatgcc aaaacaaaaa cgggttacta tgttacgggc
     8941 ttaccttggt tttctagttt atcgaccaat caggcagata agaaaagtac cgatacagaa
     9001 gtcaagctca atcagtcaga tagggtttca gagaaaacaa aaaagaaatt ggataagttt
     9061 ctcaacgttt tcttcactaa ctatacgact gaccaagata atttggactt agtggctcac
     9121 aatgtaacag ttgtaaaaaa tgccactttt aaaaccttag attttagtta ctacaaagca
     9181 accgataaag cagttatggc ttatgttcaa gtaacatttg aagtcgcagg ctcgacacat
     9241 gctgaaaact tcaccttgac cttatcagaa aaagggaagt cctattatgt ggagaaagtc
     9301 agccatacaa tccctgctaa ttacgcagat acaaaagaat aaaaggagag tttaatcaat
     9361 gagttcatct aatttacaaa catggttaca aggagacggt ttctggttca ttacctttgg
     9421 agcgattatc ctgattattt tagcttggag aaacagctct tgggggcgac tatttaccac
     9481 gcttgttttc tacgcagtga ttgcctcttt aacaggaggc ggacgacaat tacttcacgt
     9541 tatcggttgg ttcctccgtc tctttggaat tgaaacgggg ttgtgacatg gaaaaagaac
     9601 gattgtatga ttacgctaga gggataaacg ctccctactg gattcaagaa gtaaaaacac
     9661 aaaaaggaaa acgtatttgg tattttgcaa cacccatgca actttccttt tttgtggttt
     9721 ttgcactggt cttgatttta atgttgacag tgtttagctc acttcttctt ccgttgaaca
     9781 aattaacgca ttccatttca tgttatcttt attggtatat cccttataaa ctcgctaaat
     9841 tttatacaga gtatgaacca caaggaaaga aaatgcacgt tttcttatgg gattatatct
     9901 gctatcttat ggattttcgc ctcaatcgta aagcgattta tcatgaagat agaatgaaaa
     9961 tggtggatga agaagaaatt gtttttgaaa aaacagattt ataaaggagg tctaatggtt
    10021 ctaaaacttg tctatccgat tcttaaaaca caggataata tagctttaac aacagataaa
    10081 atggtgatga gtttttatcg tattcccaat actcccatca ccattacaga taaagcaaag
    10141 aaacaaaaac acaagctgac aactgctcag ctgataaaaa aactggttaa aaacaagtcc
    10201 tttgaagtct ccttgattcc aaaggactat ttgttagaag aaaagatgaa agacttttct
    10261 catgccttgt ctcccgatag tcaagcattg ggggaagaac tgttgcagta ttcgacagac
    10321 aaattgacac aagaaatgga aattccctat caatttgact ggttgattgg tgtcaatctt
    10381 ttaaaagatg accaaaacac caccgttcag gatatggcgg ttgagcgctt ctctgaagtc
    10441 tctgaaatga ttgccaatag cgtgggcttt caatatgaga ttcctgaaga ttgggcgcaa
    10501 gagtatctgg ttagtgaagc gaccgtctat caaatccttt ccgctcttcg ggcacaacgt
    10561 ttgaccaatg aagagctctt ttactatcaa cgcatgcagt acttgcgcta tattcctcat
    10621 ctaaagcaag aagtcacagc caatcgggca ctctttaatg tgactgatac ggtcttaaga
    10681 ccaatggctg ggggctttat gaagttagac agcccctatg gttcttcttt tctcaccatt
    10741 cttccgattg ctaaaacacc cattattttt aatggctttc atctagggga atttacccaa
    10801 cgctttaatt tccctgtaga actacggata aaggcagaat ttatcgaaaa gaactcttta
    10861 aaagggcgaa tggcacgttc taacattcgt tatcgaaata ttatgaatga agctgaaagt
    10921 acagatactg tccaacaaga tgaaattatt ctgggggcaa tgtctctcaa agacttgatg
    10981 aaaaaggttg gtgggaaaga agaagtcatt gaatatggag cttatctcat tgtttctgct
    11041 tctagcctat cccaattacg acgtcgccgt caggtagtct taagttactt tgaggatatg
    11101 cacgtagaag tgagtgaagc tagtcatgat acgccttatc tcttccagtc tctcctctat
    11161 ggtcaaccac ttgaaaataa gaccagaacg tggacacact atgtcactcc aagaggtttt
    11221 gcagaactaa tgccatttac caacacagct tcaggaaacc atattggctg gcatatcgga
    11281 cgggtggata attggacagg acggtgggaa agtattgaaa aggcacaaga agcgtctaaa
    11341 aatatggtgc tctttaatgc gaccgttgga aataaagaag ggattgcagg aaaacttaca
    11401 aagaatccgc atatcctaat tactggcgca acgggtgaag gaaaatcttt tttggcagaa
    11461 cttatctttc tcctaacctc tcttcaagat gtgaaatctt tgtatgttga cccgaaacgc
    11521 tctatccgaa aacactttga agcaattgct caccacccag actttgaaaa acggttcccg
    11581 cttctggctg aacatatcaa acagataaac tttgtcactt tggatagtag ggtgaaatcc
    11641 aatcatgggg ctcttgaccc aattgtcatg ttggataaag atgacgctgt ttcagtttca
    11701 aaaaacatgc tcaattatct tattttctcc aacaagcgaa tgaaggtcac aatgaagcaa
    11761 atgaccgcta tttcgagagc aattaacaaa gtggtggctc aaagacaaac gggagaaatc
    11821 gtggggttaa aacatgttct caaagagctc accaataatc cagatagcac tatctttgaa
    11881 gtaggagatt atttgacgtc cattgtcgaa aattccattt tggaattggc tttttcagac
    11941 ggtaatgtag aaggattgtc ttatgataaa cgggtgactg ttttagaggt agcggactta
    12001 tccttaccag atagtaaaga taatcaacaa gatgtcaccc cagacgataa agaaatcaat
    12061 tctacggcgc tgatgtttgc tttgggagct ttctgtacac gctttggaga actcaatcgt
    12121 tatgaagata ccattgaatt tttcgacgaa gcgtgggtac tgatgaagtc ggcagaagga
    12181 cgagccgtta tccaatccat gcgtcgtgtt ggacgtttct ttaataatgt cctctgcctc
    12241 attactcaat ccgttcatga cgctgaagga gatgatgata ccacaggctt tgggacactc
    12301 tttgccttta gagaagacaa tgaacttccc gatattttag aacacgttgg actaacggat
    12361 aatgaagaaa atctggaatg gattaaaaat atgatttcag ggcaatgcct ctataaagat
    12421 gtttacggca atctcaatat gatttccatt tataacattt ttgatagtat cgacccgctt
    12481 ctaaaaccaa tgaaagctac tgtttcttct aatcttgaaa ataaatacgc atcttaagga
    12541 ggtgagcttg tgcaagaagc atttgaaaaa ttaaaaggca tggatatttt ttctttaaaa
    12601 tcctacatga taaaaactgg ctttggagat ttcagcggtg tttgggtgat gttcaacgaa
    12661 atctttgtca acttcccttt tttcctcctc aacctcattg ttggtttctt ctctttgatg
    12721 attcggattt tagaaaacgt ccgactttat gacacttaca agcaatatgt cttcagtgga
    12781 gctcaatcca tttggcgagg ctttacagga gctacttctg gtgtggctca gtcatcatta
    12841 gtctttctga ttctcctttg cttggctttt tatctctttt atcagctcct catgtctaaa
    12901 ggcaatttct ctcaaagatt gcttcatgtc cttctggttc tctttttagg ctttggttgg
    12961 tttgggacag ttgctggaac ttctggtgga ttgtatctgc tcaatacagt agacaatgtc
    13021 gctagtacta cgattcatca catttctaat gtctccgtct cctacggaaa aaatcagtct
    13081 ttaaaaatag ggtcttccgt agctgattct tatatcgcag aaacctctta taaagcctat
    13141 ctctttgtca atacaggaca agaaaacgga aaatacaaag accgtcaaac aggaaaagaa
    13201 gaaccgtttg aggattcaaa agttctagga agcttggata aatcaggcaa gtttaaagcc
    13261 gtttctaccg ctgataggaa taaatattta gaaataggaa atggagcaag tgatgataat
    13321 gaacataacc gttgggtatc ggctattcct gattatatcc ctgttaagtt cttctatatc
    13381 ctttttaaaa tggttgaagc gattgtcatt gccattccta ttgtcttaat tcagttgctc
    13441 aatgtccttg cacaagccct tgtccttatc atgattcttc tttttcctat tgcccttttg
    13501 gtttccttta ttcccaaaat gcaagatatt ctttttggag tttttaaggt catgtttggt
    13561 ggtcttgctt tcccagccat tacaagcctg ttaactttgg ctttacttta ccttgaaaaa
    13621 atcattgaaa acttagtaag tagtggcttt gataaggtta ttggagattt ttcttccttg
    13681 tctactttta gcgctctctt tgaactaatc gtctctgttt gtgctaaagg agtgatttat
    13741 tggttgcttt ggaaatacaa aggagaattg attgaattgt tactcggttc acgagcaaag
    13801 attacgtttg atgagataga taccaaagtc aaagaaaccg ttacaaatgg gcaggaaccc
    13861 ctgaagcagc tgcctacaaa agctactaac gcctttgaaa tggctcaaac atccggaaac
    13921 tttttccttg ctggagcggg attgggagcg gggctcttca tgaatgctgg taaacagatg
    13981 aaaactcttt tttcaaatcc ccatccgact tcagaaggaa cggaagattc aacgcctcca
    14041 gaaaatgacg aggaggaaac acccactact tcttcctttg aagaaacagg cgctcccgac
    14101 gaggtaacaa caccgtttca agagccagat atgccaatgg agatttttga atctgctatt
    14161 caggaagaag acacaagcgt tcaacctttt gacacagaac cttccccgct aatggataca
    14221 aagcagaaca gagagcagaa agagtttgaa gagcgccgtc aaaatcgcct ttcttggaga
    14281 caaaaacatc acatcaataa acttgaaaag gaattagaac catacaaaga tgatgaagca
    14341 ctataccaag cgcaaggttc aaatgccttt atcaagaact atcgtaaaac cttgccgaaa
    14401 gacgataaat tgaaagccaa tattaaccgt aaacatcaga taatagagga attagcacga
    14461 ttgagaggag gaaaggaata atgaatatac gcatttattc taaaagaggt tttaacagac
    14521 ggtgaacaat gcggttgccc ttgcctataa aaagagaaag ccttctattg actttctcct
    14581 tttattttca gtaaaagaga ttgagaaaga gcaactgtta gcaactatca aagaaaatcc
    14641 tcttattttg acggctcaat ggcgatttga aacggtaatg atgactgtct atgtgaaaac
    14701 ttaggaggga agatgaagaa aaaactagta attgttagtg ctttaggtgc tctggtctta
    14761 cctttgttct tggttgttat tatcatggga gcttttgcgg gtagtacgtc ttctaatgga
    14821 caagccaaag gactacaaac caaactaacg gctaaagaag tagctcaaaa agccaatatt
    14881 tcagaagaac gggcaagtga tgtcatcaaa attctcaact atgaactctc acaagaacaa
    14941 tttactctcg caggagcttc tggaagttta gcggttgcag aacgtgaaag tggctttgac
    15001 cctaaagccg tcaatacagg tggcggtgtg gctggttatt tccagtggtc aggctggtct
    15061 tctactgtca atggcaatcg ctggtcaaag gcaaaacaaa agaaattaga ttctgatatt
    15121 gaactagacc tcttatctac agagctaaat ggctcttata gcaaagtcaa aacagaaatg
    15181 caaaaagcga ctgaccctga agaagcagct ctttattggt cagaatatta tgaaggggtc
    15241 gctttatcag acggtcaaac caaaactagt gacctgaaga agaatgcgag aaaatggtat
    15301 aacctcttca aagacacttt aacaggggat acaactaatg ccaaaacacg agacggtgga
    15361 ggcgtaactt ctgatggagt gcctgctgga tatagcctta caaaggcaat caacacttct
    15421 aactatactt ctgcgaccta tccttgggga caatgcacgt ggtttgtcta taaccgagcc
    15481 aaagaagtag gcgttaactt tgacccttac atgggcaatg gtggggattg gaagcagaaa
    15541 gcaggctacc aaaccacaca tacaccaact gaacatagtg ctctcagctt ttctcctgaa
    15601 caagctggcg cagacccaac ttacggacac attgcgtttg tggagcaggt caaatcagac
    15661 ggatctattc tcatttcaga aaccaacacc aaaggacttg gagttgtttc ttaccgcaca
    15721 tttgataaag caacagctaa ccaatttaca tacgtaattg gaaagtaaaa atttgacaaa
    15781 aaactacatt tgtcgtacaa ttgaagtaca aaagaggtga ttgtatgact aaaaaaatgt
    15841 ttcaaaagcg agtagattca gaattattag atgaagtatc ggtactatac aaatccttag
    15901 gaacttctgt aggtgacgct ttcgtgatgt tcttacaaaa atcaaaggag gtgaatggat
    15961 tgccatttga tttaaggaaa caagtccatg ctatttctga taatccttta gaaagactag
    16021 ctttggcaaa ctcaaatgtt atcaaagtga ctgcttctaa tccagaagca atcgcagatt
    16081 tctttgatga agactatcca gaatacgagg aaagacatgg acaactataa cccatatgat
    16141 attctggtag caactatcgc ttttagtgat gggacaggag ctaaaagacg tcctgttcta
    16201 gtcattgcaa ataatgaaga agttatcaga acctaccgta tcactagtca gtattcccgt
    16261 aaatcatctt atatccgttc aaaatacttt gaaataaaag aatgggaaga ggcaggatta
    16321 gtgaaaccat cttggattga taccgttcaa ctttatgatt tagacgtaga acttgttcaa
    16381 gttcgcttaa tcggaaagtt aacagaaaat gaccaacaga gatttgaaaa atttttaaga
    16441 gaaaattaag attatacgat tatattataa ataatcctaa aacttttctt tttcataatc
    16501 tccttaaggt aagggcttta tagctcttgc ttttttattg cctaaaaggg aggaaaatta
    16561 tgcaagttat tttaccagaa gaacaaacaa aagaaattca actttttgtt gcaaatctta
    16621 ttaaaaatga gattgaaacc gttaaagaag attcaggaca aattagtcct ttttta
//
LOCUS       DATRSG010000050        16425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.050, whole genome shotgun sequence.
ACCESSION   DATRSG010000050 DATRSG010000000
VERSION     DATRSG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.050"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(127..1665)
                     /locus_tag="VBD89_001496"
     CDS             complement(127..1665)
                     /locus_tag="VBD89_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7136014.1"
                     /translation="MSKQKVTATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(1758..2858)
                     /locus_tag="VBD89_001497"
     CDS             complement(1758..2858)
                     /locus_tag="VBD89_001497"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7136015.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVTSLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(2855..3214)
                     /locus_tag="VBD89_001498"
     CDS             complement(2855..3214)
                     /locus_tag="VBD89_001498"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO7136016.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(3239..4246)
                     /locus_tag="VBD89_001499"
     CDS             complement(3239..4246)
                     /locus_tag="VBD89_001499"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO7136017.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(4372..6900)
                     /gene="secA"
                     /locus_tag="VBD89_001500"
     CDS             complement(4372..6900)
                     /gene="secA"
                     /locus_tag="VBD89_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO7136018.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMATLSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGSELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(7009..7956)
                     /gene="manA"
                     /locus_tag="VBD89_001501"
     CDS             complement(7009..7956)
                     /gene="manA"
                     /locus_tag="VBD89_001501"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO7136019.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLRIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(8074..8955)
                     /locus_tag="VBD89_001502"
     CDS             complement(8074..8955)
                     /locus_tag="VBD89_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7136020.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(9023..10942)
                     /locus_tag="VBD89_001503"
     CDS             complement(9023..10942)
                     /locus_tag="VBD89_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO7136021.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRIMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTLSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            11127..12566
                     /locus_tag="VBD89_001504"
     CDS             11127..12566
                     /locus_tag="VBD89_001504"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO7136022.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENILKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRTFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            12568..13530
                     /locus_tag="VBD89_001505"
     CDS             12568..13530
                     /locus_tag="VBD89_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7136023.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIISSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(13614..14048)
                     /gene="nusB"
                     /locus_tag="VBD89_001506"
     CDS             complement(13614..14048)
                     /gene="nusB"
                     /locus_tag="VBD89_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO7136024.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(14041..14430)
                     /locus_tag="VBD89_001507"
     CDS             complement(14041..14430)
                     /locus_tag="VBD89_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7136025.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(14519..15079)
                     /gene="efp"
                     /locus_tag="VBD89_001508"
     CDS             complement(14519..15079)
                     /gene="efp"
                     /locus_tag="VBD89_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO7136026.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(15808..15948)
                     /locus_tag="VBD89_001509"
     CDS             complement(15808..15948)
                     /locus_tag="VBD89_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136027.1"
                     /translation="MLLIDVMIHLSEENEKGFTKLQFFTRQIVVGVNWYVLPKIDNYF
                     NP"
     gene            complement(16007..16306)
                     /locus_tag="VBD89_001510"
     CDS             complement(16007..16306)
                     /locus_tag="VBD89_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO7136028.1"
                     /translation="MLLPVGSVVYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFP
                     EGLNLEQVYYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT         5194 a         3230 c         2648 g         5353 t
ORIGIN      
        1 tttggtccaa atcccttaag attagaaatt ggtgattgta atagcataag attctccttc
       61 taatatcttt tattatacta aaagatcacc aaaaaggcgc ctcaagagcg cctttattat
      121 gtctaattat ttatggccaa taacataagt taattgtgca gcttgtgctg aactaaaagt
      181 acggtaagag acaataccac gtcccattgc attagattct gaaatgagaa ctgaaccatc
      241 ttttttaact tcttcaacaa tagctacgtg accgtaagtg ccatcagcac cagcttgtcc
      301 tggtgaaaat gatacagcat agcctacttt tggtgaatgt gttgtttcaa agccagcctt
      361 atgttgccaa tctccaccat tacccataaa tggatcaaat gtataaccta actctttagc
      421 gcggttaaag acataccatg tacattcacc ccatggataa gttgatgcaa tatagctagc
      481 tgtgttaacc gctgttgata agctaaaacc acttggtaaa tctgctaaac ttgctgtcga
      541 taaagctttc aaggcagcag gaatttctgg tgtccctcca tgttgatcaa tccagcggtc
      601 taaatcttcc ataatctggg cacgacgttt accagttcca gagttatctg tataataaac
      661 accaccctca gtaactgtat taagttgtcc agaagataat ttctgtgctt tcaaatcttg
      721 gatttcaaag ctagagttgt tgtttttaat aattgtattt tttgtcaaca acataattgc
      781 ttcttcatca ttataagcac tagcatggct agagtcatga tcaaaggcac cataaccaaa
      841 catattagca cctggcatct tagccactcc ttgagttccc aatgaacttt caaggacagc
      901 aatagctaca atagcacgga catctaaacc actatctttt tcccattgta ataacttagc
      961 accattgata cgattagaat catattgaat ccctaaagat tttaagtacc catctaattg
     1021 ttcagctttg ataccgtaac gatgtgacaa taaatagtga gagtaggcat cctgaccaga
     1081 ccagtgttct acgtagaatg gcgttgctgt cactggtgaa atcggtgttg ataataagtt
     1141 atcatcaaag gataaagcag gaacagaagc attctgattt gtctgaacac tttctatctg
     1201 agacggagcg ggagttattt taggctctac taatggtaag gtaggctcag gttttaattc
     1261 agtctttgtt gattgacctg gcttactgtc tgcttgacta ctgtttgtag tattagtgct
     1321 aggagtagat gctgttgaag cagaatcaga tgttttagca tcttcgttgg aagaagattg
     1381 atgtgatgaa cttgatgatg attcattgct tgatgatgtc tctgtcgtac caatctctgt
     1441 tttttctgtt gagatacttc cttcggctga aggtgaagca gtttctgaat ctgtaggttg
     1501 tgttttttcc tgtttctctg cttcagaaga actttctgtt gatgctgttg ccattgtcag
     1561 gcttgtttct ggattaatag tttctgctaa ggtcactaaa ggtgatgata gcgataagac
     1621 taaagtggat aacaacaaag ttgccgttac tttttgtttt gacatatact aatgattccc
     1681 ttctataaaa attgtcactg attagtctaa catattttta aattttttaa aacagttaac
     1741 tactgtgtca tttgttttta ataatatctc ggaatacgat cgcttaaaag acaaagtact
     1801 tcataattaa tcgtctgtct tttttgagcg acgtccgtag ttgtaatgtt acaactacct
     1861 tgttgaccaa tcaaggttac tttagttccg atagtatatt tttgaggtaa acggatagtc
     1921 atttgatcca tcgagacacg cccaataatt tcacaaagtt ctccatttac tataactgaa
     1981 aatccttgca tatctcgtgt ccatccatca gcgtagccaa tcggtactgt tccaacaaat
     2041 tcgtctccag taacttgata ggttgcgcca tagccaactt gagaaccatc gtgtagttgt
     2101 ttaacatgta ctagctcaga ctctaaactt aaagcagggt ttatagggta aggtaagtct
     2161 aaatcagttc cacttggatt taagccgtac ataacaacac ccaatctaac ggcattaaaa
     2221 attgtctcac tatgccaaag gcttgtagca gaattactag catgaacaag gtccggacaa
     2281 ttatctaatc ctgaaataaa gtctttaaaa aaagtaagtt gttgattgaa tttacagtta
     2341 tcggcttcat cagctgttgc aaaatgggtg aaaatcccct tgacagaggc accactttcc
     2401 cccaattcag aaattagctt atttccttca tctaactggc gaacacctat acgtcccatt
     2461 ccactatcaa ctttgatatg tacttctaga ccagataaat ctactgctga tgtctgacat
     2521 aaacgtaacc actccaagct agtaactgtt agagtaatat tttcattttt ggctagtata
     2581 acctgctcag gcataacaac acctaatacc aatatcattt ttacaattcc tgcttgacgt
     2641 agctcaatag cctcatctaa attggatacg caaaaaccat ctacaataga ctcaatgtat
     2701 tttgaaactt caacagcacc atgcccataa gcattagcct taactacagc aaatgttttt
     2761 ttatcacttg gaatgtgctc ttgtacagat ttaacgttat ttgcaatcgc ctctaaatct
     2821 atcaaggctc tggttggtct atgataactt gaaatcattt ttcttcctcc aaaataacac
     2881 tagcttgtgc ataattacca ctatgagata ttgaaacaaa agattttcca ttaaacggtt
     2941 cttttgtaat caaaggcgct cctttatcat ccgataaaat ttcgatatca tgaaaattaa
     3001 ctttcccaat tcctgttcca agtgctttgc tataagcctc ttttgctgcc catcgccctg
     3061 ttaaaaaaga catctgacgc ttgggattgg aaattccttt aaaaagaagc aattcttgtt
     3121 cggtcaaaac gcgttctgca aaacgttgat tacgctcata tgctttagta atcgcctcta
     3181 tctcttgtaa atcaattccg tgtccaacaa tcatgtgtaa ctcctataag ttcgatactt
     3241 acaaactatt gtaaatatcc ctaattagca tttctgtttt atcccaacct agacatggat
     3301 ctgtgataga cttaccaaaa acatctggtt tatcttgtct tccatcttct aaataagatt
     3361 ctatcaaaaa accacgaaca tagtttctaa ttttagtatg ccactgtcgg tctgcaagta
     3421 ctgatttcac aatgcgaatt tgttctaaaa attgcttacc tgaattgtca tgatttgtat
     3481 caacaacaat aaagggattt tgcaaatcag tctcttcata atgagtaata gtttctaaaa
     3541 ggttttcata atgataatta gggatacttc gatagtcggc attatgatat cctctcaaaa
     3601 taacatgtga cagcaaatta ccatctgttc taacttgctt attttgatag aataattcct
     3661 gttgattttg tgctgcatag accgcattaa acatgactct taaattacca gacgttggat
     3721 ttttcatacc tactggtgca tctatacctg aagatataaa acggtgtccc tgatcttcca
     3781 ctgaacgcgc accaatagca tagtaagaaa ctaaatcatc catcactgat aacatctctg
     3841 gataaagtaa ttcatcagct gtcaggagtc cagtctctcg aatgatgtca tagtgcatct
     3901 tacgtgcttg aaaaaaaact cctagtttgt cagaatggtg aactaagcct ttataaccat
     3961 caccattcgt tcttggctta gcggtataaa ctctcataac caaaaacatt ttatctttaa
     4021 aggcagcttc caatttagct aaacgctttg cataagttag caccgccttt ggattatccg
     4081 cagaacaagg acctactatt actagaaacc gctgatcctc accttttatg atatttttta
     4141 actcttcttc tttggctaat tttttttggc taacttctag atctaagcta gttttctgct
     4201 tcaatatttc aatattaatt ttatcactta actgatgaaa tcccatgttt tttcctaata
     4261 caacctaagt ctattaataa gctctgctat acaatatagt cgtcatacta ttagacaagt
     4321 aatgataaaa cagtcttctc agactgtttt atttttattt taaaatcatt gttattgtaa
     4381 acatctcaga ccatgacaat ttttaaactt catgccagag ccacaaggac atttatcatt
     4441 acgtttaatc ccttggtatt ctggtgattc attattcata ggtacatgtt gtgccgagat
     4501 attttgctct gctgttgtcg tagcatgttg agaagcacgt tcacgctctt gttcatgaat
     4561 ttgagctttc atcaatgtac gtgtcacatc aaactcaatc gaaccaatca tatcttggaa
     4621 catacggaaa ccttctgatt gatactcaac gattgggtta ttttgagcat aaccacgaag
     4681 tccaacggag ttacgtaatt gatctaaggc atcaatatgc tctgtccatt gattatccac
     4741 taccatcaag attaagactt tttggaattc gataatagct tctggatctt ttaatttagc
     4801 aatttgttgc tcataaacag ctaaggcacg ttcataaaga agttctttaa tttcagcctc
     4861 ttttagacca cgaagttctg aaactgtgat agcttcttca tctaataacg ccgatcttgc
     4921 aaaattaaca atagcttctg ctgcagtatt tttatcgctt ctgctatgtg catcaactgc
     4981 tcttttaatc gtacgtttaa tcatcccttt gagctctgat cctaaatcac gctcagcagt
     5041 aatcacttct ctacggttag cataaataat ctcacgttgt tcacgcataa catcatcgta
     5101 ttgcaagact tgtttacgtg tgtcatagtt attaccttca acgcggcgtt gagctgattc
     5161 aacttgtcgt gtcaacattt ttgatttaat aactgtatca tcttcagcta aattcattcg
     5221 ctcaagaacc actttaatac gatctgtacc aaagcgacgc atcaaatcat cttctaacga
     5281 aagatagaat tgtgattcac ctggatctcc ttgacgaccc gaacgaccac gaagctggtt
     5341 atcaatacga cggctttcat gacgctctgt accaataaca cataaaccac caagttcacg
     5401 aaccccttca ccaagcttga tatcagtacc acgaccagcc atgttggttg cgattgtaac
     5461 agcaccacgt tgcccagcat tcataatgat ttgagcttct ttaaagtgat ttttagcatt
     5521 caaaacttca tgtggtactc cagcagcaac taattttcta gaaattaagt ctgacgtttc
     5581 aacagctact gtaccaacca gtacaggttg tccttgttca taacgttctt tgacatctgc
     5641 taccaccgct ctaaatttag agtcaagagt tggataaagt agatctgaat gatctatacg
     5701 ttgaacagga cgatttgttg ggataggaat aacgcgcata ttataaattt cacgaaactc
     5761 ttcttcctca gtcttaccag taccagtcat tcctgctaat ttatggtaca tacggaacat
     5821 attttggtaa gtaattgaag cgctcgtttt tgattcttct tggattggca ctgactcttt
     5881 tgcttcaata gcttggtgta agccatctga gaaacgacgt ccttccatcg tacgaccggt
     5941 aaattggtcc acaattaaaa tctcttgttc ttcactaacc acataatcaa tatttaacaa
     6001 cataatatag ttagctcgaa gagcgttatc aatgtagtga gttaaggcaa cattttcgag
     6061 atcatacaag ttatttagat ggaaatagtt ttcagccttg tcaatacctg tatctgataa
     6121 accaattgtt tttgttggaa catcaatgat ataatcatcg ctattaagcg tttttacaaa
     6181 catgtccgca cgtgtataca gttggttcat ctcagagctt acaggacctg aaacaatcaa
     6241 tggcgtccta gcttcatcga ttaatacaga gtcaacctca tcaaccaaag catagttgag
     6301 tgggcgttgc accatatctt cttggcgaac aaccatattg tctcgaaggt agtcaaatcc
     6361 gacttctgcg ttagttgagt atgtgatatc acaattgtaa gcttcgcgtt tttcaaatgg
     6421 tgacttagct gctaagttaa tccctacaga taatccaagc caactataaa cctcacccat
     6481 ttcggtcgca tcacgtgttg ataagtactc attaacagta ataacatgaa cacctaatcc
     6541 tgagatagca tttagataaa caggcattgt cgctgttaaa gtttttccct caccagtacg
     6601 catttctgga atatcaccat ggtgaagaac gataccaccc ataatttgga cgtggtatgg
     6661 gtataaacct aatacacgtt tagaagcttc acgaactacc gcaaacgctt ccggtaacaa
     6721 ttgatctaaa gtttcaccat tttgataacg ttctttaaat tctggagttt ttgcttgtaa
     6781 agcttcatct gataacgtag ccatatgatc tgcgtaagag tcaactttct tagctatttt
     6841 atctaatttt tttaattcac ccttgtcatt ttcaataaca gtgcgtagaa tatttgccat
     6901 tctaattcct ttcgatactc ttatcaccac attttaccat aaataatgtt gtctttcaat
     6961 aaatggtcgt tttaatcgag attaaaacaa gtggtatccc cactctcatt attttttaac
     7021 atgacttgct ataatttcta actgtccatc aatatcccat ttgacaacat catttggcaa
     7081 gataaagtgg tcaccttttt taagtgtata aactttgtta tctactgtaa tgtgacccgc
     7141 tccgtctaag acactaacca aaaggtaggg tgcaaattgt ttaaagttgg taacaccaga
     7201 aatttcccat ttataaactg taaagaaatc atttgaaact aataaagttg aagatagatg
     7261 ttttaacttc atattggcag gaacagtatt ggcaggttta ccaatcgtga gaacatctat
     7321 tgattgttca atgcgtaaat cacgtaattt tcctgaagca tctggacgat caaaatcata
     7381 aacacgataa gtagtatcac tagattgctg tgtttctaaa attaatatcc ctttacctat
     7441 agcatgcatt gtcccacttg gaacgtagta aaaatcccct gatttaacag gaattcgggt
     7501 taaaagatgt tcccaatctc cagattcaat catttggcga agttcttctt tagttttagc
     7561 attatgacca tagataattt cagatccttc atcagcagaa ataatatacc aacattctgt
     7621 tttaccaagt tcaccttcat gtttaagagc ataagcatca tccggatgaa cctgtacact
     7681 aagccagtca ttagcatcta agattttagt tagtaaagga aagacagtat catcagggtt
     7741 gccaaataag cttttttcac cttgatacag cttatctaaa aaacaacctt taaagcgacc
     7801 attcttaact cttgagacac cgttaggatg agctgaaata gcccaatatt ctcctgttgt
     7861 ttcacttgga atatcatagc caaattcatc ccttaacttt gttccacccc agattttatc
     7921 gtgcatgctt gcttctaaaa ataatggttc cgacataaga atcccctatc ttttgtaaaa
     7981 ttcaagccta ttatagcatt ttatccatca tatcgcttac tttttttaat cttttatttg
     8041 ctaactaaac taagtagtgg cttaagggtt ttattaacct tttgatattt tctttgctaa
     8101 agcaaaattt cctaacgttg cagaaccatt ttcttctata gctggtgtca caatataatc
     8161 agacaaatcg ggaactggta aatacccatt taataaagtt gcaaatgtct ggcgaacacg
     8221 tcttaacatg tgctcttgtg ccatgacgcc tccaccaaat acaatgactt gtggacgata
     8281 taatacagtt gcttgtattg cagcctgagc aatataatag gcttgaacat cccaaaccat
     8341 tgaattttct tcaatcaatt caccacgaat accagtccta gcttctagag ttggaccaga
     8401 tgccaaacct tctaaacagc tattatgaaa cggacagata cctttaaaat catttgcttg
     8461 gtcctgcgga tgcatcgcca tataagtatg acctgcttca gtatgaccag ttcctcctat
     8521 aaattcaccc ttttgtatag ctcctgcacc aattcccgta ccaatggtgt aataaacaag
     8581 tgaatcaata ttgttacgag ctataacttc accataggca gagctattaa catctgttgt
     8641 gaaataaaat ggaacattga aatctttaga aattaagccg agtaaatcaa cattagccca
     8701 atgtaacttt ggggtcgttg tgatataacc gtatgtcttt gattttttat caatatcaat
     8761 gggaccaaaa gaaccaatag caactgcctc taacttcttt tcaaatcttt taaagaaatc
     8821 aacagtcttt ttaatcgttt cttgaggcgt cgttgttgga aattgcattt tttctacaac
     8881 ttttaattcc tcatcaccaa ctgcacaaac aaactttgtc ccacctgctt caatactacc
     8941 atataacttt gtcatctttt ttcctatcta taatctctat ctcaaagaca aaggttggga
     9001 taattatccc aacctttaat cctcacttaa catcaataat catgtcacct gtagtaacaa
     9061 caccctcttt taccaatggt ataacatcag caaaatcagc tgtattagtc acaataatca
     9121 tagctgtatt atccagacct gcttctgcaa tcttggctga atcaaaggtg ccaagaatat
     9181 cacctttttt cactttttgg tctgccttga ctttagaagt aaaccctgtg ccattcatgg
     9241 tgacagtatc aatacctata tgaatcaaaa tttctgcacc tttatctgat ttgataccgt
     9301 aagcatgacc tgtttcaaaa gcaatttgga caaaaccatc aacaggtgag taaattgtat
     9361 tacctcttgg tttaatagca aggccatctc ccataatacc tgatgaaaaa actgggtcac
     9421 ttactgtatc aagagcaata acctgtccat caagagggct aacaatcgtt tctgccctta
     9481 attggctagt ttgagtaatt gttggatcgt catctgtttc ttcaaccaaa ggactaactt
     9541 cagctactgc tttttcatct ttatcttcat atccaaacat ataagttaat acaaaagcca
     9601 aagacgtagt tccaattacc ataataaggt acttaacaat ttgaccattt aaataaagta
     9661 atgttcctgg gataatggta ataccaaaac cagttcctgc taatttcaat attgaagcta
     9721 accagccacc tgctgctcct gcaattaatc ccataataaa tggcttacca aaacgtaagt
     9781 ttactccaaa tatagccggt tctgtaatac caagtccagc tgaaagcgct gccggaaaag
     9841 caagggcttt taatttttta ttttttgttt taacaccaac tgccaacgtg gcacctgctt
     9901 gtgctgtcat agcagctgta ataatagcat taaatggatc tttcccatca gcagcaatta
     9961 actgagcttc aagtaaatta aagatatgat gaacacctgt aacaacgatt atctgatgaa
    10021 caccacctaa aataagacct gataggccaa gaggtaaatt aaggacaaat tttgtccctg
    10081 ctaaaacata gttttcaact gaatggaaaa taggaccaat gacaaaaagt gctaaaattg
    10141 acataactgt aaaagttagg aacggcacta atagtaaatc aagaacatct gggattttct
    10201 tatgcaacca tttttcaagt ttagcaccaa gtaaacctac aaagaatgcc ggcaatactg
    10261 aattttgata accaactact ggaataaagc cgaaaaattt tatgggatgg gcatcacctg
    10321 aggccactgc ccaagcgttt ggaagggcag agttgaccat catcaatcct aatacgagac
    10381 cgataacagg atttccacca aaaactctaa aagctgacca agaaatcaag gcaggaaaaa
    10441 aagcaaaggc cgtgtcagtc aaaacgacag tataggtata aaaattagaa cttgaaaatg
    10501 cttttgaagt cgtcccaaat aaagctaaaa ctgtatcatt attaatcgca ccgcgaatcc
    10561 ccataaataa accagtagca acaatggctg gcaacaatgg aacaaaaaca tcaccgaaag
    10621 tacgaatagc tcgttggaaa gcattccctt gtttagccgc ttctgccttt tgatcagagg
    10681 ttgacaaagt tggcaaacct tgagccacta cttcatcata aatcttatta acagtaccag
    10741 ttccaaaaat aatttgatat tgacctgaat tgaaaaatgc tccctgaact ttttcgatat
    10801 tttcaacagt atttttatca atgactgtct catcttttac cataatgcga agacgagtgg
    10861 cacagtgtgc tacgctacgc acattactag caccaccaat cgcattaatg acttgtttag
    10921 caatttctgt atttgaagcc atctataaat ctcctttgac attttgaaag cgtttcattt
    10981 tatttactct aatattctag catgtctcat aaatatatgt caagcgtttg acataaaata
    11041 atattttttg atgttaagag ttgatttcaa aagagaaaac gtttactata tagttattag
    11101 atattaagac agtcaaggag tatcaaatga atttacccac tgaaatccgc tatcgtcctt
    11161 atgatgaatg gactgaagaa gataaggaaa atattttaaa gaatgtctca aaatcccctt
    11221 ggagagcaac ttatcactta gaagctaaaa cagggctttt aaatgaccca aatggcttct
    11281 cttactttaa tggaaaattc catctttttt atcaaaattg gccctttgga gctgcacatg
    11341 gtttaaaaca atgggtacat acagaatcag atgatttagt tcatttcaaa gaaacaggaa
    11401 tcaagcttaa acctgaccat gtcaatgata gccatggtgc atactccggt tctgcattag
    11461 ccattgacga taagcttttt cttttttata ctggtaatgt ccgtgacatg aaatggaacc
    11521 gtgacccccg ccaaattgga gcatggatga caagtgatgg caagattaca aaatttgata
    11581 aagtccttat ttcacaacca aatgatgtca ctgagcattt tcgtgatcct cagattttca
    11641 attatgataa tcaattctat gctgttatcg gggcgcaaaa tagtaaaaaa tgcggattca
    11701 ttaaattata taaagcactt aataatgaca tccatcattg ggagtttgtg ggagatttag
    11761 attttggtgg tactggatct gagtatatga ttgaatgtcc aaatattatt tttgtcaaag
    11821 gaaaacctgt attactttat agtcctcaag gactagataa aaacgaattg gactaccaaa
    11881 acatttatcc aaatacctac aaaatcggac aatatttcga tgctaactca tctaagatag
    11941 ttgaaccatc ccctatctat aatttagatt atggttttga agcctatgct actcaaggat
    12001 ttaatacttc tgatggtcgt acctttatcg tgagttggat aggcttgcca gatattgatt
    12061 atcctagcga tcaatttgat tatcaaggag ctatgagttt agttaaagag ttaagcataa
    12121 aaaatgggaa cctttatcaa tatcccgttc ctgctatgaa aaatcttcgt caacatcaag
    12181 cagaatttaa aacacaactt caaactaaca acacctatga attagaatta ctagttccta
    12241 gaaacgatct ttcttcattt gttctctttg ctaatccgaa aggacaagga ctatccatta
    12301 ctattgatac tgcaaagggg aaagttatcg ttgaccgctc acaagccgga cagcagtacg
    12361 ccacagaatt tggtactagt cgccagtgtg acattccaaa agatgctact agtatcaata
    12421 tttttatcga taagtccatc tttgaaatct ttataaataa aggagaaaaa gtatttacgg
    12481 gacgagtctt tccagatgca gaacaatcag gaattcaact aaaagaagga cacgttcatg
    12541 gtaaatattt tgaattaaaa tattaatatg gttgcaaaat taacagatgt tgctgcactt
    12601 gccggtgtta gcccaacgac tgtatcacgc gttattaaca aaaaaggcta tttatctcaa
    12661 aaaacagtca ccaaagtcaa tgaagccatg agaacccttg gttataaacc taataattta
    12721 gcacgtagct tacagggaaa gtctgctaaa ttaattggac tcattttccc caacatccgt
    12781 aacatttttt acgctgaatt aattgaacac cttgaaattg agttgtttaa gcatggttat
    12841 aaaactatct tatgtaatag tgaaaaggat cctatcaaag aaaaagaata cctcgaaatg
    12901 ctaggagcaa atcaagtaga cggtatcatt tcaagtagtc acaacctagg aattgatgat
    12961 tacgagaaag ttgaggctcc catagtagca ttcgatagaa atttagctcc acacatcccg
    13021 atcatctcat ctgataattt ctttggagga aaaatggctg ctcaaaccct taaaaaacat
    13081 ggctgtcaaa agatgattat gattacaggg aatgataatt cagattctcc tactggactt
    13141 aggcggctag gtttttccta tgaatcaaag gaatccaaag ttatcacagt cacaaatggg
    13201 ctatctaaca tgagacgtga gatggaatta aaatcaatta tctcgactca caaaccagat
    13261 ggtattttta catcagacga cttaactgct ttattagtaa tcaaacttat cagccaacta
    13321 gggttgtcca tcccagaaga tatcaaagtg attggatatg atggtacaag tttcattcaa
    13381 gattacgtcc cacatctaac cactattaaa caacctattc gagaaattgc gcaattgatg
    13441 gttgaaatac tcctagctaa gattgagggg caaaagacaa ataaggatta tattttacca
    13501 gtcagtctca tccctggtag tagtgtttag gaaatcatat ttataaaata atttttagta
    13561 atagttattt ttcaaagcaa attaaaaatg tggaagaatc ttcttccaca tttttaattt
    13621 tcatttgtta taaattggct taataaacca ttgacaaatt ttgctgacgt ctcatctgaa
    13681 tattttttag caatctcgat aatctcattt agagctacac gatcaggcgt ttcatcaaaa
    13741 tatttaattt catagaggcc taagcgtaag agagatttat caaccaatgt tagacgttct
    13801 aagctccatc ctgattttaa gtgactagaa atcaaagtat ctaattcatc cttgtgatct
    13861 acaacaccat taacaagatt aagtaaaaag attggaacct ctaaaacttt gtcttcagat
    13921 actgttttat cataaccata agcaaagtga gccgcatcaa taaactctcc accagtttct
    13981 aagctaaata gtgtttgaaa agcacgttca cgtaaatcac gtcttgaatc tttaaataca
    14041 ctagtcatct aagaaatcct catcaaacaa tgacttcaag tctggttttg gtgttttctc
    14101 tggaacaata ccttcaacat ggatattgac cgaagaaatt tttacttctg ccatgtcata
    14161 tacagctgtt ttgacagctt gttgaatagc aattgaaact gctggtacat ttacaccata
    14221 ctgcaagtaa acataaatat ctgcagctac tgtatcatct tcttcatttt taaggtagac
    14281 acctctacct agagatttct tactaaggct atccgttacc gctttgttac gtaacgaatg
    14341 aacaccatca actttagttg cagcaatacc tgtaattacc tcaagtacac gtggtgaaat
    14401 gacaatttct ccaagatttt cagttgtcat agcttatcct ttcttttagt gtttactaag
    14461 gtttttcctt agtagagtaa attcatcatg aatctactta acactttttt gagagtcatt
    14521 aggcgcgtga aacgtaagtt ccttctgctg tattgattac taacttttgt ccagcttcaa
    14581 taaagtctgg aacgttaaca acaagtcctg tttccatagt tgcaggtttt cctgaaccag
    14641 ttacagtagc acctttgatt gatggttgag tttcagcaac tgtcaattca acagttgttg
    14701 gaatttgaac accaataact tctgttccat agaattgaat ttttacttct gagttttcaa
    14761 gaatgtagag taattcattt tcaatgttaa cagttggaat ttcatattga tcgtaagttt
    14821 cattattcat gaagtaagct gtatcatcca tcttataaag gtattgagca ggtacagttt
    14881 caataatagc ttgctcaaat ttttcttcag gacggtaact tgtatcaaaa gttgaaccag
    14941 tacgaacatc acgcaatttc atacgcatga ttgtgttacc tttacctggt ttgtggtggc
    15001 tagcttcaag aacacggatt aatttcccat cagccgtttc aaaagtcatg cctgctttaa
    15061 gcttacttgc ttcaatcatt tattaatacc tctttgtaaa aattatttat caactaattt
    15121 tatcacaaat aacctgcttt ctcaaatagt ttattgattt ggtatgtgtc tgatttcttg
    15181 ataaataaat aaaatcacca agagggcgta aataagactg aagcacttga cactttcctc
    15241 ttacttcagt tttgataaca gttttaatat tataccgata tcttgactaa gattgaaata
    15301 ctgtttaggt gtttttagtg actttttagt aaattttaat aaaggtagta aactatagtt
    15361 gtcttattga aacagataaa ataaatcacc tacggagcta ttcgtatgaa aatgtagtta
    15421 ttctcgatat aagttttaga tacgaactta atttgctcta ttgaagctgg taaaggaaat
    15481 cggggtggtg ttagtggacg tgcgctaagc attggtactg agcttggtga taccaatctt
    15541 gcttactaca tcaggtagct ctctgtttta gtgccatatg caattggaat aagagctgtc
    15601 attggtattg aaggagccct agtccactga tcattgaatt actaatttag gaggtggaat
    15661 gatgaatttt aatatgtctc attatagaaa gttacaacta tgtcgttttc taatcgtaca
    15721 gatacctgcg ctggctttat tagctattct tttatacggg attttcctaa agtacagtct
    15781 attttcaaaa gtattttttc ttatctttta ggggttgaaa tagttatcaa ttttgggaag
    15841 tacataccag tttacaccaa ccacaatttg tcgagtaaag aattgtaatt tagtaaagcc
    15901 tttttcgttt tcttctgata agtgtatcat gacatctatc aatagcatat tcacactagt
    15961 agcatattga tttgtttttg agtagaattt actttttaga ttatttctat tctacactag
    16021 tctttccctt tggaagattc tttccctctg tatttttcca tttttcaatg agacgagaaa
    16081 ctctttcttc ctcctcatca tggtaacctt caaaaacaac ctcatcaata tcttcttgat
    16141 taaaatagta aacttgttca agatttaaac cctcaggaaa aattccacct aggtaatcaa
    16201 aataaacttc ttgtccttct tgctcaacaa ttgcacctct atttacaata actaattttt
    16261 gattgccatc aataagatag acaacactcc ctactggtaa taacatactt cagttctccc
    16321 caatataata atttttccca tttaacgatg gttgaaactc aatgaacttt ataaaaaata
    16381 aactgtttga aaaaataaat ccaaataaaa ataagtttga aaagt
//
LOCUS       DATRSG010000051        16424 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRSG010000051 DATRSG010000000
VERSION     DATRSG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16424
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.051"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            109..1314
                     /locus_tag="VBD89_001511"
     CDS             109..1314
                     /locus_tag="VBD89_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO7136029.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
     gene            1433..1993
                     /locus_tag="VBD89_001512"
     CDS             1433..1993
                     /locus_tag="VBD89_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO7136030.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            1994..3946
                     /gene="gyrB"
                     /locus_tag="VBD89_001513"
     CDS             1994..3946
                     /gene="gyrB"
                     /locus_tag="VBD89_001513"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO7136031.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            4040..5764
                     /gene="ezrA"
                     /locus_tag="VBD89_001514"
     CDS             4040..5764
                     /gene="ezrA"
                     /locus_tag="VBD89_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO7136032.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KAQERIRF"
     gene            5858..6499
                     /gene="serB"
                     /locus_tag="VBD89_001515"
     CDS             5858..6499
                     /gene="serB"
                     /locus_tag="VBD89_001515"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO7136033.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(6520..7005)
                     /locus_tag="VBD89_001516"
     CDS             complement(6520..7005)
                     /locus_tag="VBD89_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7136034.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(7018..7473)
                     /locus_tag="VBD89_001517"
     CDS             complement(7018..7473)
                     /locus_tag="VBD89_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO7136035.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            7671..8978
                     /gene="eno"
                     /locus_tag="VBD89_001518"
     CDS             7671..8978
                     /gene="eno"
                     /locus_tag="VBD89_001518"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO7136036.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(9086..10150)
                     /locus_tag="VBD89_001519"
     CDS             complement(9086..10150)
                     /locus_tag="VBD89_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7136037.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
     gene            10379..11662
                     /gene="aroA"
                     /locus_tag="VBD89_001520"
     CDS             10379..11662
                     /gene="aroA"
                     /locus_tag="VBD89_001520"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO7136038.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            11655..12167
                     /locus_tag="VBD89_001521"
     CDS             11655..12167
                     /locus_tag="VBD89_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO7136039.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            12224..13597
                     /locus_tag="VBD89_001522"
     CDS             12224..13597
                     /locus_tag="VBD89_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7136040.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAIIPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            13698..15053
                     /gene="rlmD"
                     /locus_tag="VBD89_001523"
     CDS             13698..15053
                     /gene="rlmD"
                     /locus_tag="VBD89_001523"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7136041.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            15161..15382
                     /locus_tag="VBD89_001524"
     CDS             15161..15382
                     /locus_tag="VBD89_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136042.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            15500..16237
                     /gene="aphA"
                     /locus_tag="VBD89_001525"
     CDS             15500..16237
                     /gene="aphA"
                     /locus_tag="VBD89_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO7136043.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
BASE COUNT         5407 a         2532 c         3251 g         5234 t
ORIGIN      
        1 aatatttgca actcataatg atttgagttg cattttttat tgcaaaaaat ataagtcaga
       61 ttctgaaaaa atgttataat aattaccatg aataaaaaag gaagtcctat gtcacaaaaa
      121 tcaaactatt ttaaagggca aattgattat gctgttgtga taccagtatt ttttttatta
      181 atgattggtt tagcctcaat ttatgttgca acgatgaatg attatccttc taatatctat
      241 atcgcgatgt ttcagcaggt gtcttggatt attatggggt gtattattgc atttgtcgtt
      301 atgcttttca gtactgaatt tttatggaaa gccacgccct atctgtatgc tttaggacta
      361 accctaatgg ttcttccctt aattttttat agtccacagc tttttgcggc aacaggtgct
      421 aaaaattggg tgaccattgg ctcggtaact ttgtttcaac cgtcagagtt tatgaagatt
      481 tcctatattt taatgttatc tcgaataact gttagttttc atcagaaaaa tcgcaaaact
      541 tttcaagatg attggaagtt gctaggattg tttggtttgg ttacattgcc agtgatgatt
      601 cttctaatgt tacaaaaaga tttaggaaca gcattagttt tccttgccat tttgtcaggt
      661 ttaatccttt tatcgggcat atcttggtgg ataatcttgc caattttatc tactatcgtt
      721 ctttttatag ctagcttttt gatgattttt atctcaccaa acggcaagga atggttttat
      781 aatttgggga tggatacata tcaaatcaac cgtttatctg cttggattga tccatttagt
      841 tttgcagact caattgctta tcaacaaaca cagggtatgg tatcaattgg aagtggcggg
      901 gtaacaggta aaggttttaa tattttagag ctctcggttc ctgttcgtga aagtgacatg
      961 atttttactg tcattgctga gaattttggc ttcattggtt cggcgattgt tttagggtta
     1021 tatctcatta tcatttaccg tatgttaagg atcaccattg aatctaataa tcaattttat
     1081 acttttatat caactggttt tatcatgatg attgttttcc atgtttttga aaatattggt
     1141 gcagcagttg gtatattacc tctaacagga atcccacttc cgtttatttc gcaaggtgga
     1201 agttcattac taagtaatct tattggaatt ggattggtct tatccatgtc ttatcaaaat
     1261 acagttcgtc aggccaaggc taatggatca catttgaagt atcgtagtat ataacaaaaa
     1321 aaggttggaa agataatcca gccttttaat aatcgaaata ttttagagga gtgagttaat
     1381 taagtaaggt ggtagctata aaaaatcgta ataatatgat ataataaagg gtatgaatta
     1441 tcatgattat atttgggatt tgggtggtac actacttgat aattacgaaa gttccacacg
     1501 tgcttttgta gagactttga aagaatttgg atatcaagca gaccacgata gtgtgtatca
     1561 aaaattaaaa gagtcaacag cagatgcaat tgcatatttt atccctgaag aagctgattt
     1621 tttaaaagag tataaggcta atgaagctaa ggttttggaa acaccaattc tattccaagg
     1681 ggcaaaagaa cttttagcga aaattcaaag acaaggtagt cgtaattttt tggtgtctca
     1741 tcgtgataat caagttatag ttattctgga aaaaacagaa attattgact attttacaga
     1801 agtagtgact gctgataacg gcttttcaag gaaacctagt cccgagagta tgctttattt
     1861 gaaagaaaaa taccaaattg ataattgttt agtaataggc gatcgtgata tagataagca
     1921 agcaggagaa tcggctggtt ttgatacctt attagtcgat gggtcaaaat cattgatgga
     1981 gataatagag taaatgacag aagaaacaaa gaatatggaa caacgtgctc aagaatatga
     2041 cgcaagtcag attcaagtat tagaaggttt agaagctgtt cgcatgcgtc caggcatgta
     2101 tatcggttcg acttcgaaag aagggcttca tcaccttgta tgggaaattg ttgataactc
     2161 aattgatgaa gccttagctg gatttgcagg acatattaaa gtttacattg aaccagataa
     2221 ctctattaca gttgttgatg atggtcgcgg tattccggta gatatccaag aaaaaactgg
     2281 aagaccagct gtagagacag tttttacagt tctccatgcc ggtggtaaat ttggtggtgg
     2341 cggatacaag gtttctggtg gcttacatgg tgtagggtca tctgttgtta atgcattgtc
     2401 aacacaatta gatgtcaagg tttataaaaa tggcaaggtt cattatcaag aataccaacg
     2461 cggtgttgtt gttaatgatc ttgaaattat cggtgatact gacttatcag gaacaacagt
     2521 tcactttacg cctgacccag aaattttcac tgaaaccact gtatttgatt ttgataaatt
     2581 agccaaacgt attcaggaat tagctttttt aaatcgtgga ctaagaatct ccatttcaga
     2641 caagcgagaa ggtcaagaag ttgaaaaaga atatcactat gaaggtggta taggttcata
     2701 cgttgagttt atcaatgaga acaaagaagt gatttttgaa aatcctattt atactgacgg
     2761 tgaattagat ggtatttcag ttgaagtagc aatgcagtat actactggat atcaagagac
     2821 tgttatgagt tttgcaaata acatccacac tcatgaagga ggaacgcacg aacaaggatt
     2881 tcgtactgcc ttgacacgtg tgattaatga ttacgctaaa aagaataaga ttctcaaaga
     2941 aaatgaagat aatttaactg gagaagatgt tcgtgaaggt ttaacagcag ttatatctgt
     3001 taaacatcca aatccacaat ttgaaggaca aactaaaaca aaacttggaa attctgaagt
     3061 tgttaaaatt acaaatcgtc tctttagcga agctttcaat cgattcctat tagaaaatcc
     3121 acaagtagct aagaagattg ttgaaaaagg aattttggca tctaaagccc gtattgcagc
     3181 aaaacgggcg cgtgaagtaa cacgtaaaaa gtcaggctta gagatttcaa acttacctgg
     3241 gaaactggct gattgctcat ctaataatgc tgagatgaat gaacttttta tagtggaggg
     3301 agattctgcg ggagggtctg caaaatcagg acgtaatcgt gagtttcaag caattttacc
     3361 gattaggggt aagattctaa acgttgaaaa agctacaatg gataaaattt tagcaaacga
     3421 agaaatccga agtctcttca cagctatggg aactggattt ggtgcagatt ttgatgtatc
     3481 aaaagtcaga tatcaaaaat tagtcattat gactgatgcc gatgttgatg gagctcacat
     3541 cagaacttta cttttaactc tgatttatcg ttttatgcga cctgttttgg aagcaggata
     3601 tgtttatatc gctcaaccac caatctatgg tgtcaaagta ggaagtgaga ttaaagctta
     3661 tattcagcca ggggtcaatc aagaagagga attacgtcaa gctctagata cctatagttc
     3721 aggacgttca aaacctactg tacaacgtta caaaggtcta ggtgagatgg atgaccatca
     3781 actttgggaa acaacaatgg atcctgaaaa ccgcttgatg gcacgtgtct cagttgatga
     3841 tgcagcagaa gcagataaga ttttcgatat gctaatggga gatcgagtag aaccacgacg
     3901 tgaattcatt gaagctaatg ctgtttatag taacttagat atctagtcca aagatagctt
     3961 tgaaaaacag ttcaagtatg ctataatctt aacgttatag tagattttta gaaaattaga
     4021 ccattataag gagagttgaa tgtcgagcgg aatcatattg ctgcttgtgg ctatagtcct
     4081 gcttgtgatt attgcctacg tagtcggcgt cgtaataaga aaacgtaatg atacattaat
     4141 tgcaaactta gaaactagaa aacaagagtt agttgacctt cctgtgcaag aggagattga
     4201 gcaagtcaaa ttgcttcatt tgattggcca aagtcaatca acttttagag aatggaacca
     4261 aaaatggact gatttatcaa ctaattcatt taaagatatt gattttcatt tagttgaagc
     4321 tgaaaatctt aatgattcat ttaattttgt gcgtgctaag cacgagattg ataatgttga
     4381 tagtcagcta actattatag aagaagatat tgtttcgatt cgtgaagctt tagaagtttt
     4441 aaaagagcaa gaagagaaaa atagtgcccg tgtaacacat gctttggacc tatacgaaac
     4501 acttcaaaaa tcaatttctg aaaaagaaga taattacggg acaaccatgc cagaaattga
     4561 aaagcagttg aaaaatatcg aggctgaatt ttcacatttt gtgaccttaa actcaactgg
     4621 tgacccgatt gaagcttctg aagttttgaa taaagcagag gagcatacga ttgctttagg
     4681 gcaaattaca gaacaaattc cagctattgt tgctaaatta gaagacgatt ttccagatca
     4741 gttagatgat ttggagacag gatatcgtcg tcttttagag gaaaactacc atttccctga
     4801 aaaggatatt gaacaacgtt ttcaagaagt tcgtgaggct attcgttcga attcagatgg
     4861 tttagttagt ttggatctag atcgtgctcg cgatgaaaat gaacatattc aagaaaaaat
     4921 tgataaactt tatgatattt ttgaacgtga gattgctgct tataaagtag ctcataagga
     4981 tagtaaaatt attcctcaat tccttgctca tgcaaaatct aacaatgaac aactggggca
     5041 tgaaatcaaa cgattatctg ctaaatatat cttaaatgaa aatgaaagct taagtttacg
     5101 ttccttcaca aatgatttgg aagaaattga aacaaaggtt ttaccttctg tagaaaattt
     5161 tggtcaagaa gcttcacctt atactcatct acaaatttta tttgagcgta cgttgaaaac
     5221 attgactact gttgaagaaa atcaaatgga agtttttgaa gccgttaaaa ctattgaaag
     5281 cgttgagaca cgagcacgcc aaaatatgga taagtatgtt aacaagctcc atatgattaa
     5341 acgtttcatg gaaaaacgca atcttccagg aatcccgcaa gacttcttaa gcactttctt
     5401 tacaacaagt tctcaaattg aggcacttat taatgaactc agtcgcggtc gtattgatat
     5461 tgaggctgtt tcacgtttaa atgatgtgac tacaaatgct atcgctaatt tagagcaagc
     5521 tacatatcta gttgttcaag atgcgactct cacagagcag ttactacagt attcaaaccg
     5581 ttatcgtagt tttgaacaaa atgtgcaaaa gagctttgaa caagctttgt acttatttga
     5641 agttgaacat aactataaag catctttcga tgaaatttca tacgcacttg agactgtgga
     5701 accaggtgta actgaccgtt ttgtgacgtc ttatgaaaag gcacaagaac gtattcgttt
     5761 ctaaaaaaga agagttagta ctcttctttt tttaattgct atctatgaaa ggtgctaatt
     5821 ttaaggtaca atagtaaaaa agttttggag gttttaaatg attaaaggtt tgttagtcat
     5881 ggacgttgat tcaaccttaa taatggaaga agctattgat ttattagcta ttgaagcagg
     5941 ggtggggaaa caggttgctg cactgactga cgctgcaatg cgtggcgagt tagattttga
     6001 agaagctttg aagaaaagag tagcgctact taaaggtttg cctgttacta ttttgacaga
     6061 tatattatcc tcaatacatt ttactccagg ggcttatgaa ttgattaaag agtgtcacaa
     6121 gcgtcagatg aaagttggtc ttgtatcagg aggttttcac gagacgattg atatcttagc
     6181 taagcaatta caagttgatt atgtcaaagc gaatcgtctt ggtgtcaaag gtggttttct
     6241 cactggcgaa gttgagggag agattgtcac taaagaagtg aaaaaaataa agttaaaaga
     6301 gtgggcttct gaaaatcact tagacctttc acaaaccatc gcaatgggag atggtgccaa
     6361 tgatttacca atgatcaaat ccgctggggt aggaattgct ttttgtgcaa aaccaattgt
     6421 cagagaagaa gctgcgtatc agattaatga aaaaaatttg cttaaggtat ttgatattat
     6481 tgatgcaaat gaaaactagg cctttattaa gcctagtttt tagattaagg attttaagag
     6541 ttttatttga gagggtatta atttgttttt agataaaaat aaatcaatat attctggttt
     6601 taaccagata tagtcaacgg tctctccttc ttgcaatgta attatatctt ttgcaccatt
     6661 gtattttgcg ataaaatggt ctacaaagta agaacgctca gagaaccact ctctattaag
     6721 aaaggttaga cattgaggaa cgataccagt ttcttctttg agttccctta gaatagcttg
     6781 cttagcattt tctcctttaa gggcagatcc gccagcggtg gcttcgaaat aagcaggaaa
     6841 agctttcttt ttagggtgcc gtttcatcat caatacgtca ccatcctcat gaaaaaggat
     6901 aacatcaaca actaagtgga ataagccttg tgggatattt tgatccctat taagtgtaag
     6961 acctgtaatg aacccatatt ggtcataagc atcccaaatt tccattagac ctccttgtta
     7021 tttttgacta aagaaatgtt gccatagact ttttttagga gactcctctt tttttggtga
     7081 attgaataaa tcagggaatt gtgtctcaaa atcgttccat tcaacgtttt cagccttatc
     7141 actatgtata ataataccaa atggagaatt ggcatttgct tcattaacaa tcgtagcagc
     7201 tagtgataga cggttagcta acttcatgta gaacatttga acatcagtag ggagatttgg
     7261 tgataatttt aaactaagat taggatattc aagtatcaga ttattaagag cagatgttat
     7321 agcaagttga aacttttctt cttgtgcctc ttttaaactt aagtggagca caactctttc
     7381 tttataagta ccaaggtagt aacgttgttg atcaggatca agacgatttt caccgcttgc
     7441 tttttgtagg attttattgt caagttcagt cataatttct ccttgaattt gctaatttta
     7501 ttataaccta aatgggttgc attagcacag gtaaacttga aaagaattag tgattttttt
     7561 atcaaaaacc acgtaataaa gcggtttcac tgggtttttc cttgttatta actaaagatt
     7621 atgatattat gaatatgtaa aaaattaaaa ctcataagga gagtattgta atgtcaatta
     7681 ttactgatgt ttacgcacgc gaagtccttg actcacgcgg taacccaaca cttgaagtag
     7741 aagtttatac agaatcaggt gctttcggac gtggtatggt tccttcagga gcttctactg
     7801 gtgaacacga agcagttgaa cttcgcgacg gcgacaagtc tcgttacggt ggtcttggaa
     7861 cacaaaaagc tgttgacaat gtaaataacg ttattgcaga agcaatcatt ggttatgatg
     7921 tacgcgacca acaagctatc gaccgtgcta tgatcgctct tgacggaact cctaataaag
     7981 gtaaattagg tgcaaatgct attcttggtg tttctattgc cgtagcacgt gctgcagctg
     8041 attaccttga agtaccactt tacagctacc ttggtggatt caacactaaa gttcttccaa
     8101 ctccaatgat gaacatcatc aacggtggtt ctcactcaga cgctccaatc gcattccaag
     8161 aattcatgat tatgccagtt ggtgcaccaa ctttcaaaga agcacttcgt tggggtgctg
     8221 aagtattcca tgctcttaag aaaatcctta aagaacgtgg tcttgaaaca gctgttggtg
     8281 atgaaggtgg atttgcacct aaatttgaag gtactgaaga tggcgtagaa actattctta
     8341 aagctattga agcagctggt tacgaagctg gcgaaaacgg aatcatgatt ggttttgact
     8401 gtgcttcatc agagttctac gatgcagaac gtaaagtata cgactacagt aaattcgaag
     8461 gtgaaggtgg agcagttcgt actgctgcag aacaaatcga ttaccttgaa gagttggtta
     8521 acaaataccc aatcatcact attgaagatg gtatggatga aaacgactgg gatggttgga
     8581 aagctcttac agaacgcctt ggtggacgtg tacaattagt tggtgacgat ttcttcgtta
     8641 caaatactga ttacttagca cgtggtatca aagaagaagc tgctaactca atccttatca
     8701 aagttaacca aatcggtact ttgacagaaa ctttcgaagc tattgaaatg gctaaagaag
     8761 ctggttacac tgcagttgta tcacaccgtt caggtgaaac tgaagattca acaatcgctg
     8821 atatcgcagt tgcaactaac gctggtcaaa ttaaaacagg ttcattatca cgtactgacc
     8881 gtattgctaa atacaaccaa ttacttcgta tcgaagacca acttggtgaa gttgctcaat
     8941 acaaaggtat caaatcattc tacaacttaa aaaaatagtc tcagattatt aaacagttga
     9001 aaatataata aaaaccatct tgcatatgca agatggtttt tatgtacaca ttataataat
     9061 agaattgatt tgataataaa cagttctacc attgcaatcc agcagcagcg atttctgctt
     9121 ttgaagcgta ttgtccattt ggacgatgcc accttcctct atagtcacgt ttgtagcctt
     9181 gactagtttg ttcttgtact agagtttctt gttttttctg agtttcagct gcgagccttg
     9241 cttgctcctc tttttgcttc ctttgagctt cagcagcaag ccttgcttgt tcttcttcca
     9301 gttttttctg agtttcagca gcagcttttt cctcttcaac tttcttttct gcaagtcgtt
     9361 tttcttcttc gtgcttagcg ataagttttc tttgttcttt agctgataag acagtatttt
     9421 gagcctttcc gctgaggtag tcaatggtag catttggtga ggtattctct aaggtaacag
     9481 tgctgatgcc gaattgatct tgagtggatt tgtttccgat aaaaataggt aataatttcc
     9541 cagtcttatc aataccaaca tattttaatt caatttttct tggaattagt tcattatttt
     9601 gataaattgg tgttacttta taatctaagt agtagttttt atgagtcgaa agccaagtct
     9661 ttagttgttt ttcatagaat agcatgctct cagggttaga tgaattcgtg ctattgtagt
     9721 ttccagtatt tagccaattc gtcattggaa ctaaattctt tctttcattg tttagtccac
     9781 taaattggtg gcaaataaga cggcctcggc tcattagcca agctttttga gtaccatcat
     9841 tataatagaa tttaaaattg tgccatccaa caggggttgt ttttaaacgt tttactctct
     9901 ttttcttggg ttcatcttta acttttagct gaatatgtgc gaatgtggca cgacctttgt
     9961 catccaactc tcctaaaacc aattgtcgtt tgtgtttaaa aggtaaagta ccattctctt
    10021 ttgttttata ctcttgagca gtaacaatat tttctgtggt atgatttgta gctaggttta
    10081 ttggtgtgta aagcgaaata accgttagta aggttgcaat tcctaatttt ataaactgtt
    10141 ttcttttcat ctgttaacct ctaaaataac ataaaatagt agctgtttat ttttaaatgc
    10201 attctgaaaa ttaaaatgtg atttttgtaa caatattata acataatgat gtatgacatg
    10261 tgaagttaat ttataaaaga tgttacagct atatagagga gctatagtat aaataaaaaa
    10321 ttcaaaatat agcatatatt atgatacaat aatagcaaaa tagattttgg gagttatcat
    10381 gaaattactg actaatgcaa atactctcaa gggaaccatc cgtgttcctg gcgataaatc
    10441 aataagtcac agggctatta tttttgggag tatatctcag ggagttaccc gaattgttga
    10501 tgtcttaaga ggcgaggatg ttttatcaac tatagaggct tttaaacaaa tgggggttct
    10561 aattgaagac gatggcgaga taatcactat ctatggcaaa ggatttgcag gtttaacaca
    10621 accgaataat ctcttagaca tgggaaactc tggtacttca atgcgtctga tagcaggtgt
    10681 gttagctggt caagagtttg aagtgacgat ggttggagac aatagtttgt caaagagacc
    10741 tatggatcgt atagcattgc ctttaagcaa aatgggagct agaatttcgg gagtaactaa
    10801 ccgtgattta ccaccattaa aacttcaagg tacaaaaaaa ttaaaaccta ttttttacca
    10861 tttacccgta gcttctgcac aagtaaaatc agctttaatt tttgcggctt tacaaactaa
    10921 gggagaatct ctcattgtag aaaaagagca aacaaggaat catactgagg acatgattcg
    10981 gcaatttggt ggacatttgg atattaaaga taaagaaatt cgactcaatg gcggtcagag
    11041 cttagtagga caagatataa gagttcctgg tgatatttcg agtgctgcct tttggatagt
    11101 agcgggtctt attattccta acagtcatat tattttagaa aacgtaggta taaacgagac
    11161 acgaactgga atccttgatg tcgtcagtaa aatgggtgga aaaattaagt tatcttcggt
    11221 tgataatcaa gtaaaatcag caacccttac agttgattac tctcatctac aagctactca
    11281 tatttctggg gctatgattc cacgtctgat tgatgaatta cctattattg ctcttttagc
    11341 aacacaagct caaggcacca cagtcatagc agatgctcag gaattaaagg tgaaagagac
    11401 agatcggatt caggtcgttg tcgaatcgct aaaacagatg ggtgctgata ttactgctac
    11461 agcagatggt atgattatac gtggtaatac ccctcttcac gctgcttcac ttgattgtca
    11521 tggagatcat agaataggaa tgatgatagc tattgctgct ctcttggtca aagaaggcga
    11581 agtagattta tctggtgaag aagctattaa tacaagctat cctaactttt tagaacattt
    11641 ggagggatta gttaatgcct aaggtattat tgggattcat gggagttggc aagactagtg
    11701 ttgccaactg cttagaaaat gaagtaattg atatggatag tctgattgaa aaacatattg
    11761 gtatgtcaat ttcccgattt tttacagaag aaggagaagc ctcctttcgt gctttagaat
    11821 ctcaattttt aaacgaactt ttaaagaaaa aaaatgaagg acttgtgata gctagtggag
    11881 gaggtattgt actccttgaa gaaaatcgtc gtcttttaac tttaaatcgt cataataata
    11941 ttcttttaac aggaagtttt gaagtcttat atcatcgtat aaaaaaagat gagaagaata
    12001 gaagaccatt atttttaaac cattcaaaag aagaatttta tgatatttat caaaaacgca
    12061 tgttactcta tagtggtttg tctgatatga ttattgatac cgattatctt acaccacaaa
    12121 aaatagctac tgtaattgga gagtcactgg ttaatttatc taactaagtt atcacagttt
    12181 ggtagtatga actattatta ttgaaagtaa atcacctgaa aatatgtcaa gaaataatta
    12241 cggtcagcta aatcatcatg aagagctacg atataactat cttttaaaaa acatccatta
    12301 tcttaatgaa cgtgaaaaga tggaatttca gtaccttcat tataaaaaaa cagcagtacg
    12361 accacagaga aggacagaaa gtccaccaac taattcttat tatgaggaac cttattctga
    12421 ttcttattat caagatgacg atttttactc agagcctcag ctaacctcac aaggtttacc
    12481 aatttatcaa gaagagagag ctccgaagaa gaaaaaacaa agggccagaa aagaaaaaca
    12541 gagggtgaag gttatggcgc ctttccctcc aaaggctata atccccccta gaaagaaaaa
    12601 aaaatttaag ggtttcctaa agttcatagg tattatatta ctaattgttt tatcaggtat
    12661 ggttttcatg tttgttaaag gaatgcgtga tgtgaataat gggaagtcac attatagccc
    12721 tgcaattatt gaagatttta agggaaaaga tgctgttgat gggactaata tcttaatttt
    12781 aggtagtgat aaacgtgtca gcgagagatc tactgatgcg agaacagata ccataatggt
    12841 tgctaatgtt ggtaacaagg ataataaggt taagatggtg agttttatgc gagatctctt
    12901 agtcaatatt cctaattata gtacggaagg ttactatgat atgaaattaa atgcgtcttt
    12961 taatttggga gaacaagata atcataaagg agcagagtat gttcgacaaa cgttgaaaaa
    13021 tcattttgat attgatataa aatattatgt tatggttgac tttgagacat ttgcagatgc
    13081 tattgataca ctgtttccta atggtgtcaa gattaatgct aaatttggac ttgtcggtgg
    13141 acaatctgct gattcagtta aagttcctga tgatttaagg atgaaaaatg gcgttgtacc
    13201 tagccaaaaa attaaggttg ggattcagta catggatgga aggacccttc taaattatgc
    13261 tcgtttcaga aaagacgatg atggggactt tggtcgaaca caacgtcaac aacaagttat
    13321 gagagctatt gtctctcaaa taaaagaccc acgtcgtctc tttacaggtt ctgctgctat
    13381 cggtaaagcc tatgctttaa cttcaagtaa tttatcttat agctttgtcc taacagatgg
    13441 cattccaatt ttatcagatg ctaaaaatgg tattaagcaa atgacaattc caagagaagg
    13501 agattgggta gatgattatg accagtatgg tggtcaaggg ttaacaattg attttgctaa
    13561 gtacaaaaag atcctaaaga aaatgggact gcgttaacat ttttaattat gatataatag
    13621 agtgatttat tgtcactcta tttttatctt tagtgtataa actaaagact tcaaatgata
    13681 gatataaaag gaaaattatg ttacataaaa atgatattat tgaaactgaa attagcgaca
    13741 ttagccatga gggaatgggt attgccaaag tagatggctt tgttttcttt gttgaaaatg
    13801 ctcttccagg tgagattatt aagatgcgcg tcttgaaatt acgtaagcgt ataggatatg
    13861 gaaaagttga ggaatattta acgacttctc ctcaccgaaa tgaaggatta gactatacct
    13921 atttacgtac agggattgct gatttgggac atcttactta tgaacaacag ctgttattta
    13981 aacaaaaaca agtagctgat aacctttata aaattgctca tatatctgat gttttagtag
    14041 aacctactct tggtatgaca atccctttgg catatcgaaa taaagctcag gtaccagttc
    14101 gtcgtgttga tggacagcta gagacaggtt ttttcagaaa aaattcacat actctggtat
    14161 ccattgaaga ttatttaatt caggaaaaag agattgatgc tctgattaat tttacacgag
    14221 atttattaag aaaatttgat gttaaaccat atgacgaaga gcagcaatct ggtttaatta
    14281 gaaatctcgt tgttcgtcga ggacattata cagggcaatt aatgttggtt ttggtgacaa
    14341 cacgtcctaa aatattccgc attgaccaaa tgattgaaaa gttggtgtct gcctttcctt
    14401 ctgttgtttc aattatgcaa aatattaatg atcgtaatag caacgttatt tttgggaaag
    14461 aatttcgtac tctatatggt tctgatacta ttgaggacca aatgttagga aatacttacg
    14521 ctatatcagc ccaatctttt taccaagtta atactgaaat ggctgaaaaa ctgtaccaaa
    14581 aagcaatcga tttttcagat ttaaactcag aggatatcgt tattgatgcc tattcaggta
    14641 taggaacaat tggcttatct gttgctaaac aagtaaaaca tgtttatggt gttgaagttg
    14701 ttgaaaaagc agtaagtgat gccaaagaaa atgctactcg aaatgggata actaacagta
    14761 cctatgttgc tgattcggct gaaaatgcaa tggcaaagtg gcttaaagaa ggtataaaac
    14821 caactgttat aatggttgat ccaccacgaa aaggattaac ggagagtttt gtatattctg
    14881 ctgcccaaac aaaagcagat aaaatcactt atatttcttg taattcagcg acaatggcgc
    14941 gtgatatcaa actatttgaa gaattagggt atcatttagt aaaaattcaa ccagtagact
    15001 tattcccaat gacacatcat gtggagtgtg tggctctgct cgtaaaagcc tagaaacttt
    15061 ttaacgaaag gaatattaaa aagccttaga taatcgactg acctattacc aaaaaatcga
    15121 aggggctcta cttgccattg cacaaacacc aggaaatagt ttgccaacta tgcagctgtt
    15181 agatgagtta gttcttccaa aagacattac catagcagat gtcagtcaaa gtatccaaga
    15241 atttgaaata gaaagagatg cccttcaaga attttttgac gatatgctga agacttatac
    15301 aacctaccaa gaaatgaaag attatattaa atctcgtgaa gcgaacactc ctaagcaaga
    15361 gacagaagaa aaatcacttt gaagactgta gacttaacat aagtaaaaaa taaccatata
    15421 tgatatcgct ttcattctta tgttttttgt gataaactaa ttaaagaaat aatttttata
    15481 ctttattatg gaggttctta tgaagaaagt gttagtgagt agtcttttgg ttttagggat
    15541 tacgataacg ttacaaacag tagttgaggc taaggggcca aaagtagctt atacacaaga
    15601 gggaatgact gctctttcgg acacaaataa agataaagtc actactattt ctattgacga
    15661 gattcaaaaa agcttagaag gtaagaagcc gattactgtt agttttgata ttgatgatac
    15721 actgcttttc agtagtcaat attttcaata tggtaaagaa tatgtaactc ctggatcgtt
    15781 tgattttctt cataaacaaa aattctggga tcttgttgca aaacgaggag atcaagattc
    15841 cattcccaaa gaatatgcta aaaaattaat tgctatgcat caaaaacgag gagataaaat
    15901 tgtttttata acaggtagga caagagggtc aatgtataag gagggcgagg ttgataaaac
    15961 agctaaagcc ttagctaaag attttaaatt agacaaacca attgctgtaa attatacagg
    16021 cgataaacct aaaaagccat acaaatatga taaatcatat tatattaaga aatatggttc
    16081 agacattcat tatggagata gtgatgacga tattcatgca gctagggagg ccggtgctag
    16141 accaattaga attttaagag cacctaattc tacaaatcta cctttaccag aagctggagg
    16201 ctacggtgaa gaggttctcg aaaattcagc ttactagtat ctttattagt taacatagta
    16261 ttatctatca atttttagat ttgtaataag aagtgctaaa aaagtagcaa tcaatgatat
    16321 gctaataagg gaagttggag tagtttagat tgattattaa tgactgatct tctctcctct
    16381 cggtaacaag aagtttttct aacaaatgcc tgtctaaaaa aatt
//
LOCUS       DATRSG010000052        15217 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRSG010000052 DATRSG010000000
VERSION     DATRSG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.052"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            47..799
                     /locus_tag="VBD89_001526"
     CDS             47..799
                     /locus_tag="VBD89_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7136044.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     AENKSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRDADNKSQVGQL
                     IGKNPLFSKPTVSRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNNSRTISVINKL
                     PKTGGDQNVIFKLVGFGLILLTSRCGLRRNEN"
     gene            1007..2083
                     /locus_tag="VBD89_001527"
     CDS             1007..2083
                     /locus_tag="VBD89_001527"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO7136045.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            2253..2591
                     /locus_tag="VBD89_001528"
     CDS             2253..2591
                     /locus_tag="VBD89_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136046.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            2722..4263
                     /locus_tag="VBD89_001529"
     CDS             2722..4263
                     /locus_tag="VBD89_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7136047.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            4303..5535
                     /locus_tag="VBD89_001530"
     CDS             4303..5535
                     /locus_tag="VBD89_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO7136048.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            5748..6449
                     /gene="pyrF"
                     /locus_tag="VBD89_001531"
     CDS             5748..6449
                     /gene="pyrF"
                     /locus_tag="VBD89_001531"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO7136049.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            6462..7091
                     /locus_tag="VBD89_001532"
     CDS             6462..7091
                     /locus_tag="VBD89_001532"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO7136050.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            7103..8395
                     /locus_tag="VBD89_001533"
     CDS             7103..8395
                     /locus_tag="VBD89_001533"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO7136051.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            8560..9483
                     /locus_tag="VBD89_001534"
     CDS             8560..9483
                     /locus_tag="VBD89_001534"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO7136052.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQCLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            9497..10573
                     /gene="carA"
                     /locus_tag="VBD89_001535"
     CDS             9497..10573
                     /gene="carA"
                     /locus_tag="VBD89_001535"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO7136053.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVGTSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTFDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            10604..13786
                     /gene="carB"
                     /locus_tag="VBD89_001536"
     CDS             10604..13786
                     /gene="carB"
                     /locus_tag="VBD89_001536"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO7136054.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNISDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYSTVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRAGRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESKAFTLKVLD"
     gene            13912..14235
                     /locus_tag="VBD89_001537"
     CDS             13912..14235
                     /locus_tag="VBD89_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136055.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIWSLN
                     REENE"
     gene            14232..14702
                     /locus_tag="VBD89_001538"
     CDS             14232..14702
                     /locus_tag="VBD89_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO7136056.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLKIIEGSQIIDFHQKRS
                     FMVLEVNDNIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHGKCSVR"
     gene            14680..>15217
                     /locus_tag="VBD89_001539"
     CDS             14680..>15217
                     /locus_tag="VBD89_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO7136057.1"
                     /translation="MVNVQSGNISNYQKNLLDSATNFQNIFPALVNQYSSSITANESL
                     KKSLEASDNMFNDLMTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNELLEKQL
                     SDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSEALKSQD
                     NIAKDQSEQLDKIVREGLA"
BASE COUNT         5107 a         2437 c         3008 g         4665 t
ORIGIN      
        1 taataaaatg acttttaaaa tttaaaaaaa gtaaggagaa aattaattgt tcaataaaat
       61 aggttttaga acttggaaat caggaaagct ttggctttat atgggagtgc taggatcaac
      121 tattatttta ggatcaagtc ctgtatctgc tatggatagt gttggaaatc aaagtcaagg
      181 taatgtttta gagcgtcgcc aacgtgatgc ggaaaacaaa agtcagggta atgttttaga
      241 gcgtcgccaa cgtgatgcgg aaaacaagag ccaaggcaat gttttagagc gtcgtcaacg
      301 cgatgttgag aacaagagcc aaggtaatgt tctagagcgt cgccaacgtg atgcggaaaa
      361 caagagccaa ggcaatgttt tagagcgtcg tcaacgcgat gttgagaata agagccaagg
      421 caatgtttta gagcgtcgtc aacgtgatgc ggaaaacaaa agtcagggca atgttctaga
      481 gcgccgccaa cgtgatgcgg ataacaagag ccaagtaggt caacttatag ggaaaaatcc
      541 acttttttca aagccaactg tatctagaga aaataatcac tctagtcaag gtgactctaa
      601 caaacagtca ttctctaaaa aagtatctca ggttactaat gtagctaata gaccgatgtt
      661 aactaataat tctagaacaa tttcagtgat aaataaatta cctaaaacag gtggtgatca
      721 aaatgtcatt tttaaacttg taggttttgg tttaattttg ttaacaagtc gctgcggttt
      781 gagacgcaat gaaaattaag tataatcaat catttagtaa ctattataat gatatataca
      841 atcaataaaa aggaatcgga tacgagattc ctttttataa ttaggttggt tagggtgact
      901 tttttcattt ggctattctt gaaagtttat aaaaatgtag tataatagtc acattaaaat
      961 gttttgaaaa tattgatgaa taacatcaac aaatagaggt cattatatgg gatataccgt
     1021 tgctatcgtg ggtgctacag gtgccgtagg aacacaaatg attcgtcaat tagaacaatc
     1081 gaatttacca atagaacaag tgaaactttt atcatcaagt cgctcagcag gtaaaatttt
     1141 acattttaaa gatgaggcta tacgtgttga agagacaaca aaagaatcat tttacgatgt
     1201 tgatattgcc ttgttttcag ctggtggatc tatttcagca aagttcgctc cttatgcagt
     1261 aaagtctgga gcagttgtag tagataacac gtcatatttt cgtcagaatc ctgatgttcc
     1321 actagttgtt cctgaagtaa atgctcatgc catgattggt cataatggta tcatagcttg
     1381 tcccaattgt tctactattc aaatgatgat tgctttagag cccattcgtc aaaaatgggg
     1441 gatagagcgt gttatagttt ccacctatca agctgtttcg ggttcaggtg cacgtgctgt
     1501 tgaagaaact aaggaacagt tgagacaagt tttaaatgat aacctttcac ctgatcaatt
     1561 aattgcaacg gtcttgccat gttctagcga tcaaaaacat tatcctatcg cattcaatgc
     1621 attacctcaa attgatattt ttactgataa tgattatacg tacgaagaaa tgaaaatgac
     1681 tttggagaca aagaaaatta tggaagatgc tactattaag gtttcagcta cctgcgtccg
     1741 cattcccgtt ttatcgggtc attctgagtc tatttatatt gagacgaaag aattagcctc
     1801 aatctctgaa attaaaaagg ctattgcaaa ttttcctggt gccgtcctac aagaccttcc
     1861 atctcagcaa atttaccctc aagcgattaa tgctgtaggc catagggaga cctttgttgg
     1921 tcgtattcga aaagaccttg atcaagaaaa tggtgttcat atgtgggtag tttcagataa
     1981 tctactgaag ggggcagctt ggaattctgt tcaaatcgca gaaacattgc ataagaatgg
     2041 gctggttaaa ccagcgaaag agttaaaatt tgaattaata tagaagaata tgacattgct
     2101 taatacttat actgacttct agtgatatta atttttcatc aatatcaggt aaaacacttg
     2161 acaaattatg aaaacatttg cataatgaaa ttaacaaatg aaaatgattg caaatcgttt
     2221 taacattgtt tccacattaa ggaggggctc ttatgcaaat catcaaacgt gatggacaga
     2281 ttgttgaatt tgatcctgaa aaaatctacc aagctatctt gaaagctgct caaacagttt
     2341 atgtgcttga tgagtcactt cgtcaagacc ttgctcaagt gactaaaaaa gtaatcttag
     2401 atttagagga tgctcaggtt gaacgaccaa cgatcaatat gattcagtct ttggttgaaa
     2461 atcgtttatt ggatgcagga tacattcata ttgctgaaca ctatatttca taccgtttgc
     2521 aacgtgattt agagcgaaat ggttatgatg atcaaatcac tgttcattta cactttgaaa
     2581 aaattaagta aggtttgact tttaagttta aactctctat acttgtatat ggaacttact
     2641 atgtaatgtg ctaatttaaa aacttgtaga gtgtctctat gagtttttta ataacctcta
     2701 ttatcaatca aaggatttta aatgagtatt ttagaagtaa aaaacttaag tcacggtttt
     2761 ggtgatcgtg ctatttttga gaatgtttct tttcgccttt taaaaggtga acatatagga
     2821 cttattggtg ctaatggtga aggaaaatca acattcatgt caattgttac aggtcatctt
     2881 caacctgatg aaggaaaaat tgaatggtca aaatatgtaa ctgctggtta ccttgatcaa
     2941 catactgtgt tagaagaagg tcaaacagtt cgtgatgttt tgcgaacagc atttgatgaa
     3001 ctttttaaaa cagaggcacg tatcaatgat atatatatat ccatggcaga tgatggcgca
     3061 gatgtagata gccttatgga agaagttgga gaactacaag atcgtttaga atcacgtgat
     3121 ttttatacgc ttgatgctaa aattgatgaa gtcgcacggg cattaggagt tatggatttt
     3181 ggtatggatt ctgatgttac tgaattatct ggtggtcaaa ggacaaaggt ccttttagca
     3241 aaactgttat tagaaaagcc agatatcctc ctgcttgatg agcctacaaa ctaccttgat
     3301 gctgaacaca ttgattggtt aaaacgctat ttacaaaatt atgaaaatgc ttttgtttta
     3361 atctcacacg atattccatt tttgaacgat gttatcaata ttgtctatca tgttgaaaat
     3421 caagatttag taagatattc cggtgattat actaattttg aatcagtcta tgcaatgaaa
     3481 aaggctcaat tagaagcagc ctatgaacgt caacaaaagg aaatagctga tttacaagat
     3541 tttgttaatc gtaataaagc acgcgttgca acacgtaata tggcaatgtc tcgtcaaaag
     3601 aaactagaca aaatggatat tattgagtta caagcagaga aaccaaagcc aagttttgaa
     3661 tttaaagaat ctcgcacacc aggacgtttt attttccaag ctaaagatct tcaaattgga
     3721 tatgatcgtg ctttaacaaa acctctaaac ctaacatttg aacgtaatca aaaaatagct
     3781 attgttggag caaatgggat aggaaaaaca acattactta agagtttact aggtattatc
     3841 ccccctattt ctggtaatgt tgaacgaggt gattttattg atttaggtta ttttgaacaa
     3901 gaagtacccg gagggaatcg ccaaactcca cttgaagcag tatgggatgc ttttcctgct
     3961 ttgaaccaag cagaggttag ggcagctctt gctcgttgtg gtttaacttc aaagcatatt
     4021 gaaagtcaga ttcaggtgtt atctggtggg gagcaatcaa aggtcagatt ttgcttatta
     4081 atgaatcgcg aaaataatgt ccttgtactt gatgaaccaa ccaatcattt agatgtagat
     4141 gctaaagatg agttaaaacg tgctttaaaa gcttataagg gatcaatctt gatggtatgt
     4201 catgaaccgg acttttatga aggatggatg gatgatgttt gggactttaa ccaacttagt
     4261 taagtggcta gggtaataaa gtagaaggca gagccaaata agatgcaaaa tgatatgagc
     4321 gttgttctta gagctggtaa attactgatt gaaagcggag cagaagtcta tcgtgtcgaa
     4381 gacacaatga agcattttgc taaggctctt cagatagaaa attttgaagc ttatgtggtt
     4441 agtagcagta ttatcgcttc cgggattaac cgctatggta aacaagaagc taaagtatgc
     4501 aatacagatg gtgtaacagc aaatttaggt agacttgaag ctgttaataa cctttctagg
     4561 caaattgcta aacaagactt agtatctcca gaagaaattg taaagcagtt agacttaatt
     4621 gaacatcaaa aagattatag tttgcttgtt actttaattt cttatttttg tggagcgggt
     4681 agtttttctt tagccttggg tagttcctta ttagattcat tttccgctgc tgtaacaggc
     4741 ttaattttag gatatttttt gaatttgatg gaaagtcgta tacatacagg atttcttttg
     4801 accattctag gtagttctgt agtggcttta agtgctaatt tattatattt ttcaggatta
     4861 ggtgagcatc gtagcattat catattaggg gcattaatgg ttatggtgcc aggggctgcc
     4921 tttgttaatt cggtgagaga attttcacag aataattttt caacaggttt agctttgatt
     4981 atgtctgctt tactcatttg tatttctata tcagcaggcg tggctattac aattgaaatt
     5041 attccgtttg cagaacaaat gacaggttct ttttctggtg ttcctaatac cattcttgag
     5101 attatcatca gaacactaat ggccggttta gggacgatag ccttctctat tctctaccat
     5161 gttccaaaac gttatttcct tgacttaggg atactaggag caatatcttg gatgctctac
     5221 ctgatcttat ggcaacaatt tcacatggat gctatagccg tattttttcc agggcttttc
     5281 atcacttatt tttcacggct attagctgct aaacgtaagt gtcctgccac tgtcttttta
     5341 gcgacaagta tgtttcctct tatcccagga cttagcttct atagagccgt ttacttttta
     5401 ttgacgggag cagatgcctt agcaatggaa tattttagat cctgttttgt aacagctttt
     5461 acaattgcta tagcaatcag tattgtacag cagattcctc tatcgttctt tattagacgt
     5521 aaaatgataa aataatttag tatttatttg acaaaatgat tgtaaacttg tagaatacct
     5581 acaaatgtat ctttaagtga gtccagagag gcgaacaagg tcaccatcat aagtcaaata
     5641 gcgagtgctt tttcgctaac tttttatgaa accttgccct caggcaaggt tttttgtgtc
     5701 atactggtac atttccaaca attattattt ctatagagag gaattatatg ttagaaaaat
     5761 gccctatcat tgccttagat ttttctgatt tggcttctgt aacaactttt ttagaacatt
     5821 ttccaaaaga agaattgctt tttgtcaaaa taggaatgga gctttattat tccgaaggtc
     5881 cctcaattat tagatatatt aaatccttag gacatcgcat ttttttggat ttaaaattac
     5941 atgatatccc taatacagtt cgattatcta tgtctgtctt agccaaatta ggcattgata
     6001 tgactaatgt ccatgctgca ggaggagtcg aaatgatgaa agcagctaga gaaggtttgg
     6061 gggagggtcc aatactatta gcagtgacac aattaacctc gacttcacaa gaacaaatgc
     6121 aggtggatca acatattaac ttaagtgtag ttgattcggt ttgccattat gctcaaaaag
     6181 ctcaagaagc aggcttagat ggagtagtcg catcggctca agaggtaaag caaatcaaga
     6241 aacagactaa cgaacatttc atttgcttaa cgcctggtat cagacctcca cagaccaatc
     6301 agttagatga tcaaaaaaga acgatgactc ctgagcaagc acgtatagtt ggggctgatt
     6361 atatcgttgt gggaagacct ataacgaaag cagaaaatcc atatcaagca tatctagaca
     6421 taaaagaaga atggaatcgt attaaataaa ggagtactga gatggattta gcaagacaga
     6481 ttgccatgga actattagat attcaagctg tttacttaag accccaacaa ccctttactt
     6541 gggcttcggg ggtcaaatcg ccaatttaca ctgataatcg tgtaacctta tcgtatcctg
     6601 agacaaggac actgattgaa aatggttttg ttaaacaaat acagaaacat ttcccaaacg
     6661 ttgatatcat cgcaggaaca gcgactgcag gcataccaca tggcgctatt atagctgata
     6721 agatgaactt gccctttgcc tatatacgct cgaaggcaaa ggatcatggt gtggggaatc
     6781 aaatcgaggg acgtgtttac tcaggccaaa aaatggttat cattgaagat ttgatttcaa
     6841 caggaggatc tgttttagag gctgttacgg ctgcgcagag tcaaggtatt gaggttttag
     6901 gggtggtagc gatatttaca tatcaattag ctaaggctga acaagcattt cgtgaagctg
     6961 atattccttt ggtgactttg acggattaca atcaattaat taaagttgct aaagttaatg
     7021 gatatattac agctgaccaa ctggtattac tgaaaaaatt taaagaagat caaatgaatt
     7081 ggcaaagtta gagaggacag atatgtatat cattaaaaac ggattgatta tcgaccctca
     7141 aagtggtttt aatcaagtat cagacatgct gattgatcaa ggaaaaatta aacaaatatc
     7201 gaaagaaatt gatattaaag gcattccaat cattgatgca agcaataaaa ttgtagctcc
     7261 aggattagtc gatattcatg tacattttcg tgagcctgga cagacacata aagaaaatat
     7321 ccatacagga gccttatcag ctgcagcagg tggttttacg acagttctta tgatggccaa
     7381 tactaaccca acgatttcta gctcagaaat tgtaaaacaa gttaaggaga gtgcagcaaa
     7441 agaagcaatt aagattgaaa cagttgctac aattactaaa tccctgaatg gtaaagattt
     7501 agttaatttc gaagaattat tagaagctgg agttgctgga ttctcagacg atggtatccc
     7561 tttgacagat actaaggttt tgcaagaagc tatgaactta gctagaaaac atgatgttgt
     7621 cctatcgtta catgaagagg atccatcgtt aaatggtgtt cttggtatca acgaacatat
     7681 tgctcagaaa atttatcatg tctgtggagc tagtggtctt gctgaatatt caatgattgc
     7741 gcgagatgct atgattgctt accaaacaca ggcgaaagtc catatccagc atttgtcaag
     7801 ttcagaatca gtagaagtag ttgattttgc gcagaagctt ggtgctaact taacagcaga
     7861 agtaaccccc caacattttt caaagacaga ggacttactt ttaactaagg gtgctaatgc
     7921 aaaattaaat ccaccactgc gacttgaaaa ggatagacaa gccctcattg atggtcttaa
     7981 aagtggtgtt atttcaatta ttgcaagtga tcacgctccc catcatatta tggagaaagc
     8041 agcagataat attagtcaag cgccctctgg tatgactggc ttagagacat cattagcctt
     8101 agggattact tatttagtat ctactaaaga actctcaatg attgactttt tggcaaaaat
     8161 gacatgtaat cctgcacagt tatatggatt tgatgcaggt tatctcagag aaggtggacc
     8221 tgcagatatc gttatttttg atcaagctga agagagaata ataaaggcag aatttgcctc
     8281 caaatccagc aactctcctt ttatcggtga taaattgaag ggagttgttc actacacaat
     8341 ttgcaatggt gaaatagtct atcaaaaaga tagccacgca gcgacattgg tataggtaaa
     8401 acatttctta ttaatatcct ttaaggtatt gtccagagag gcaaacaagg aattgcgtta
     8461 acaaatagta agttagttcc ctatctagct tactattttg cagagcctcc ttgttttagg
     8521 aggctttttt ctagctaaaa tttatagaaa gaaattatca tgaatcatca aacacaaaca
     8581 ctttcattag aacattttgt cagtttagaa gaattatcaa atcaagaagt tatgtctttg
     8641 ataaagagaa gtatcgaagt taaagaaaat cctagcaaca ttggttttga taaagattac
     8701 tatgtgtcaa atctcttttt tgagaattca acaagaacac ataagtcatt tgaaatggca
     8761 gaactcaaat taggattaaa aacaattgaa tttaatgctg atacaagttc tgttaataaa
     8821 ggagaaaccc tttatgacac tatcttaaca atgagtgctc taggattgga cgtttgtgtc
     8881 attaggcacc ctgatattga ctattataaa gaattgattg ctagtccgaa cattcattca
     8941 gctattgtca atgggggtga tggctcaggc caacatccaa gccaatcact tttggatttg
     9001 gtaactattt acgaagaatt tggatacttc aaaggactaa aaattgcgat tgtaggagat
     9061 ctaactcact cacgggtagc taaatctaac atgcaagtgc taaaacgatt aggagctgaa
     9121 attttctttt ctggacccaa agaatggtat agtagtcagt tcgatgaata tgggcaatgt
     9181 ttaccaatag atcagttagt tgatcaaatt gatgtcctta tgcttttacg tgttcaacac
     9241 gaacgtcatg atggtaaagg ggtgttttca aaagaaagct atcatcaaca atttggtctt
     9301 acaaaagaac gctataaaca tttaagggat acagcaatta ttatgcaccc agcacccgtc
     9361 aatagggatg tagagattgc tagcgattta gtagaagcag ataaggctcg aatagtgaaa
     9421 cagatgagta atggcgtcta tgcaaggata gctatcttag aagcagtgct caatagcaga
     9481 taataaagga gataaaatga agcgtctatt attattggaa gatggtagtg tgtttgaggg
     9541 tgaggctttc ggagcggatg taggaacaag tggtgaaatt gtttttagta caggaatgac
     9601 agggtatcaa gaatctataa cagatcaatc ttacaacggt caaatcatta cttttactta
     9661 tcctcttatt ggaaattatg ggataaatcg agacgattac gaatctatta gaccaacatg
     9721 taagggagta attgtttatg aatgggcaga gtatccaagt aactggcgtc aacagatgac
     9781 atttgatgaa tttttaaaat taaaaggaat acctggaatt tcaggtatag atactcgagc
     9841 attaacaaaa attattcgaa aacatggaac aatgaaagct tgtttaataa acgagggaaa
     9901 ttctattcat gaagcactag aaaaccttca aaaaagtgtt cttttaaata atcaaattga
     9961 acaagtatca accaaattag cttatgcttc gcctggagtt ggcaaaaata ttgtattagt
    10021 tgactttggt ttaaaacatt ctattctaag agaactgtca caacgtcaat gtcatattac
    10081 agtcgtccct catactacga cagcgcaaga aatcttaaat ctcaatccag atggagtact
    10141 cttatctaac ggccctggga acccagaaca attaccgaat gctttacaaa tgatccaaga
    10201 aattcaaggt aaaattccaa tttttggtat ttgtatggga catcaactat ttgctaaagc
    10261 caatggtgca aagacttata aaatgacttt tggtcatcga ggttttaacc atgctgttcg
    10321 tcatttgcaa acaggacagg ttgattttac aagtcaaaat catggttatg ctgtctcgag
    10381 agaagatttc cctgaggctc tcttcattac acatgaagag attaatgata aaaccgtcga
    10441 aggtgttcga cataaatact atcctgcctt ttcagtgcag tttcacccag atgcagcacc
    10501 agggccgcat gatacatctt atctttttga tgaatttatt aatatgattg atgatttcca
    10561 gcaaaaaagc tagctttaaa atatagaatt agaggagttt gatatgccaa agcgcacaga
    10621 catacgtaaa attatggtta tcggttctgg cccgattgtg attggacaag cagcagagtt
    10681 tgactattct ggtacacaag cctgtctctc tttaaaagag gagggatacc aagttgtttt
    10741 ggtcaattcg aatccggcta ctatcatgac agataaagat attgctgata aagtttatat
    10801 tgaacctatt accttggagt ttgtcacaag aattctcagg aaggaaagac ctgatgctct
    10861 ccttccaact cttggaggtc aaactggatt gaatatggct atggctctat caaaaaatgg
    10921 tatcctagaa gaattgaatg ttgaactatt agggaccaaa ttatcagcca ttgataaagc
    10981 tgaagatcgt gatttattta aacagttgat ggaagaactc aatcaaccta ttccagaatc
    11041 cgagattgtt aattcggtag aagaagctat tcaatttgca gagcaaatcg ggtatccatt
    11101 aattgttcgt cctgccttta ctttaggagg tacaggtgga ggcatgtgcg ataatcaaga
    11161 acaattggtt gacatcacga cgaaggggtt aaagttatcg cctgtgacac agtgccttat
    11221 tgaacgttca attgctggct ttaaagagat tgaatacgaa gttatgcgtg atgcagcaga
    11281 taacgctctt gttgtttgta atatggagaa ttttgatcct gttggtatcc atacagggga
    11341 ctctatagtt tttgcacccg cacaaacgtt atctgatgtg gaaaatcagt tattacgaga
    11401 tgcgagttta gatattataa gagctttgaa aattgaagga ggatgcaatg ttcaactagc
    11461 ccttgatccg aatagtttta aatactatgt cattgaagtt aatccaagag tatcaagatc
    11521 ttctgcttta gcttcaaaag ctactggata tccaattgct aaattagcag ctaagatagc
    11581 tgttggtttg acactagatg aggtaattaa tcctattaca aaaacgactt acgctatgtt
    11641 tgaacctgct ttagattacg tagtcgcaaa aatgccgaga tttccttttg ataaatttga
    11701 aagtggcgat agaaaattgg gaacacagat gaaagctaca ggcgaagtca tggcaatagg
    11761 acggaatata gaagaatctt tgttgaaagc ttgtcgttca ctagaaatag gtgtcgatca
    11821 cattaaaata gcagatttag ataatatatc agatgatgtt ttattagaaa aaataagaaa
    11881 ggcagaagat gatcgcttat tttatctagc agaagcttta cgtagacatt atagtattga
    11941 aaaattagca agtttgacta gcattgattc tttcttcctt gataagttaa gagtgattgt
    12001 agagttagaa gatctattat ctaaaaacag actggatatc aatattctca aaaaggttaa
    12061 aaataaaggt ttttcagata aagctatagc gagtttatgg caaataaacg aagatcaagt
    12121 tcgtaacatg cgaaaagaag caggaattct tccagtttat aagatggttg atacatgtgc
    12181 ggctgagttt gattcagcaa cgccctactt ttattcgact tatgccgtag aaaacgagtc
    12241 attgatatca gataaagcct ctattttggt tttaggatcg ggcccaatcc gaattggaca
    12301 gggagttgaa tttgattatt caacggttca ttctgttaaa gccatcagag agtctggttt
    12361 tgaagcgatt atcatgaact ctaatccaga gacggtctca acggatttct ctatttcaga
    12421 taagctttac tttgaacctt tgacttttga agatgttatg aatgttattg acttagaaaa
    12481 acctgaaggg gtcatcttac aattcggtgg tcagactgct attaatctag caaaagattt
    12541 aaacaaggca ggggttaaaa tactaggcac acaattagaa gatttggatc gtgctgaaaa
    12601 tagaaaacaa tttgaagcca ctttacaagc tctaaatatc cctcaaccac caggttttac
    12661 cgccactacg gaggaagagg cagttaatgc agcgcaaaag attggttatc ccgtgttggt
    12721 tagaccatca tatgttcttg gtggacgagc tatgaagata gttgaaaacg aagaagattt
    12781 acgtcattat atgacaaccg cggtaaaggc aagcccagat catccagtgc ttatagatgc
    12841 ataccttata ggtaaagagt gtgaggttga tgctatatcc gatggtcaga atatactgat
    12901 tcctggaata atggaacata tagagcgagc tggtgttcat tcaggagatt ccatggctgt
    12961 ataccctcct caaacattgt ctgagaccat tatcgaaact attgtagatt atacaaaacg
    13021 cttagcaatt ggattaaact gtatcggtat gatgaatatt cagtttgtaa tcaaggatca
    13081 gaaagtatat gttattgaag ttaatcctcg tgctggtaga acacttccat tcttatcaaa
    13141 agtaactcac attcctatgg cccaagtagc aacaaaagtt atcttaggag ataaactctg
    13201 caattttaca tatggttatg atctctatcc agcttcagat atggtccaca ttaaagcccc
    13261 tgtctttagt tttaccaaac ttgctaaagt agatagttta ttaggtcctg aaatgaaatc
    13321 aacaggcgaa gtgatgggat cagatattaa tcttcagaaa gccttgtata aagcctttga
    13381 agcagcatat ctccatatgc cagactatgg taacattgtt tttacagtag atgatacaga
    13441 caaagaagaa gctttagaac ttgcaaaagt ctatcaaagt attggttatc gtatttatgc
    13501 tacccaaggg actgcaattt attttgatgc taatggttta gagacagttt tagtaggtaa
    13561 gttaggagag aatgatcgaa atcatattcc tgatttaatt aaaaatggta aaattcaagc
    13621 agttatcaac acagttgggc aaaataacat tgataaccat gatgctctca tcatcagacg
    13681 ttctgcaatt gaacaaggag ttcctctatt tacatcttta gatactgcac atgcaatgtt
    13741 caaagttctt gaaagcaaag catttacatt aaaagtacta gattaatcaa acaatcaagg
    13801 taaaattaac ttccagcaaa acagaatcgg actagattaa atatagtttg ttctgttttt
    13861 ataaaaaagc ctaattgtga taaaataggg aacaaactta aggagttaat tatgttaaaa
    13921 acacgaacac atattaatga attagaaaca aaaatcgccc ttgatttagt acacagatat
    13981 ctaggtattc aacaacttgt tttttcaaag tggagacatt ctttagaaac tggttcatgg
    14041 tcttctatta taacaataaa tggcgctaat attatcaatt acattgttga tgatattagc
    14101 gacagtgata gtatttcagg tattatttgt catcctaaaa gttttcatct cagacctaga
    14161 gaaaaaaagg aacctattca gtctattgat gagttagata tcatctggtc tttgaataga
    14221 gaggaaaatg aatgattcca gatagtagtt ttaaagtgct atcagatatg gcatataata
    14281 ttgaaaaatc caaagcagaa agtaatggac accttaaaat tatagaaggt agtcaaatta
    14341 ttgatttcca tcaaaaacgc tcttttatgg tattagaggt taatgataat atcgataatg
    14401 gtatgcaggc tatggcatgt cagtctttgg atgagagtaa tcatattgtt atagcatatt
    14461 caggaacgaa cgcttttaat aagaaagata tcttaacaga tatcgattca gttgtattgg
    14521 ggaagaacaa taaattgcta acttgtcctt caagtattac cctgagagag gatggcattt
    14581 ctctgccttg taaaaatcca agtcaaatta caacatctaa actatttgca caaaaaatta
    14641 agatgaacta tcctgatgca accctctggt taacaggcca tggtaaatgt tcagtcaggt
    14701 aatatctcaa attaccaaaa aaatctttta gattctgcaa ctaatttcca aaatatcttt
    14761 ccagccctcg ttaaccagta tagtagttcc attactgcta acgaatcact gaaaaaatct
    14821 ttagaagctt ctgataacat gtttaatgat ttgatgacaa cccagacaaa tactggaaaa
    14881 gatttatcaa gcttaataga acagcgccat caagatagca tttcgtatga agcgttttcg
    14941 gcctcattac tagaaatgaa taacgagtta ttagagaagc aattatctga tattatcaca
    15001 caagcacaaa aagaccaaga aacattatca tcacaactca atagtattat gggtgatgat
    15061 aacgataatg atcataatca taaagaaaac tcatcagctt atctaaatgt tgcaaggcaa
    15121 aaaatccaag aactatctga agcactcaag tcacaagata acatcgctaa agatcaaagt
    15181 gaacaactag ataaaattgt tagagaggga ctagcaa
//
LOCUS       DATRSG010000053        15062 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRSG010000053 DATRSG010000000
VERSION     DATRSG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15062
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.053"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            88..954
                     /locus_tag="VBD89_001540"
     CDS             88..954
                     /locus_tag="VBD89_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO7136058.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            1000..1917
                     /gene="pstC"
                     /locus_tag="VBD89_001541"
     CDS             1000..1917
                     /gene="pstC"
                     /locus_tag="VBD89_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7136059.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            1907..2794
                     /gene="pstA"
                     /locus_tag="VBD89_001542"
     CDS             1907..2794
                     /gene="pstA"
                     /locus_tag="VBD89_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7136060.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            2806..3609
                     /gene="pstB"
                     /locus_tag="VBD89_001543"
     CDS             2806..3609
                     /gene="pstB"
                     /locus_tag="VBD89_001543"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7136061.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            3621..4379
                     /gene="pstB"
                     /locus_tag="VBD89_001544"
     CDS             3621..4379
                     /gene="pstB"
                     /locus_tag="VBD89_001544"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7136062.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            4413..5066
                     /gene="phoU"
                     /locus_tag="VBD89_001545"
     CDS             4413..5066
                     /gene="phoU"
                     /locus_tag="VBD89_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7136063.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            5212..7761
                     /locus_tag="VBD89_001546"
     CDS             5212..7761
                     /locus_tag="VBD89_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO7136064.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            7923..8603
                     /locus_tag="VBD89_001547"
     CDS             7923..8603
                     /locus_tag="VBD89_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7136065.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            8587..9900
                     /locus_tag="VBD89_001548"
     CDS             8587..9900
                     /locus_tag="VBD89_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7136066.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            9989..10321
                     /locus_tag="VBD89_001549"
     CDS             9989..10321
                     /locus_tag="VBD89_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO7136067.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            10339..11904
                     /gene="ffh"
                     /locus_tag="VBD89_001550"
     CDS             10339..11904
                     /gene="ffh"
                     /locus_tag="VBD89_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO7136068.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            11971..12657
                     /locus_tag="VBD89_001551"
     CDS             11971..12657
                     /locus_tag="VBD89_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO7136069.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            12647..13420
                     /locus_tag="VBD89_001552"
     CDS             12647..13420
                     /locus_tag="VBD89_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO7136070.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWVLLV"
     gene            complement(13453..15048)
                     /locus_tag="VBD89_001553"
     CDS             complement(13453..15048)
                     /locus_tag="VBD89_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7136071.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYNQTGSVKALTNQKIRQALNLATDRKGVVKAAVDTGSTPAESLVPKKLAKLP
                     NGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKNAVDYVKS
                     TWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFTTNSAYN
                     YGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGKKGLQNS
                     KLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         5004 a         2621 c         2744 g         4693 t
ORIGIN      
        1 tactatttgt aaatcattaa acactcaatg attttagtaa tatatatcaa ctactaaata
       61 tcaaaattta ttcaaaagaa taggaaaatg aaaatgaaaa aaaacatttt tttgttaacc
      121 ttgctagcca ccgtcttatt gacgctgtct ggttgtgcta attggattga taaaggtcaa
      181 tcaatcacca gcgttggttc aaccgcctta cagccacttg tagaagctgc agcagatgag
      241 tttggtaaga caaacctcgg taagactatc aatgtccaag gaggaggctc aggaacaggc
      301 ttatcacaag tacagtctgg agctgttcaa attggtaata gcgacttatt tgcagaagaa
      361 aaagatggta ttgatgctaa gaaattgaaa gaccatcaag tcgcagtagc tggattagct
      421 gttattgtta ataaaaaagt taatgttaaa aatctaacca cccaccaact tcgtgatatt
      481 tttgcaggaa aaatcaagaa ttggaaagaa gtgggaggac aagatttaga tatttccatc
      541 atcaatcgag cagcaagttc tggatcccgt gcaaccttcg ataatactat tatgggaaat
      601 gttgctccta ttcaaagcca agaacaagat tcaaatggta tggtgaaatc aattgtttct
      661 caaacacctg gtgcgatttc atacctcgct tttgcatacg ttgataaatc tgtcggaaca
      721 ttaaaactaa atggttttgc gccaacagct aaaaatgtta ctaccgacaa ctggaaactt
      781 tggtcatatg aacatatgta tacaaaaggt aatgaaacag gtctaacaaa agaattctta
      841 gactacatga aaagtgataa agttcaaagt agtatcgtac aacatatggg atatatctct
      901 attaacgaca tgaaagttgt taaagatgct gaaggtaaag taaccccaaa ataaaactta
      961 atcacatcta gataaagcat cttgatccta ggaggaatta tgaaaaatca agaattagct
     1021 aaaaaattaa catctccttc aaaaaactct cgattagaaa aatttggtaa aggaataact
     1081 tttctttcgc ttgccctaat cgtcttcatt gttgcgatga ttttgatttt tgttgctcaa
     1141 aaaggactat ctacattttt tgttgatggc gttaaattaa cagacttctt atttaataca
     1201 aaatgggaac caagtgctaa gtcattcggt gctcttccaa tgatagctgg atcatttatt
     1261 gttactattc tatcagctat cattgctaca ccatttgcta tcggtgctgc cgtatttatg
     1321 actgaaatct cacctaaata tggttctaaa attttacaac ctgcagttga attactagtt
     1381 gggattcctt ctgttgttta cggttttatt ggtttacaga ttattgttcc cttcgttcgc
     1441 tctatttttg ggggaacagg ttttggcatt ttatctgggg tctgtgtcct cttcgtcatg
     1501 attctaccaa ctgttacttt catgacagta gatagcctta gagctgtgcc acgtcactat
     1561 aaagaagcaa gtttagccat gggggcaact cgttggcaaa caatttggcg tgttatatta
     1621 aacgctgcac gtccaggaat tttcactgca attgtttttg gtatggcacg tgcatttggt
     1681 gaagctctag caattcagat ggttgtcgga aactctgcaa ttttaccaac atcattgaca
     1741 acaccagcag caacattaac gtctgttcta acaatgggta ttggtaatac tgtaatggga
     1801 actgtacaaa acaatgtcct ctggtcgcta gccttagtac ttttaattat gagtttagca
     1861 tttaatacag ttattaagtt aattacaagg gaaggaaaga aaaactatga acgctaaaaa
     1921 ggctgacaaa ttagcaacta caatactcta tagtattgca gctatcatcg ttacaatcct
     1981 cgcctctctt ttaattttta ttcttgttcg cggattgcca cacgtatctt ggtcattttt
     2041 aactgggaaa tcatcatcat atgaagcagg aggcggtatt ggaatacaat tatacaattc
     2101 ctttttccta cttattgtta ctctgattat ttctattcct ctatctttag gagctggaat
     2161 atacctctca gaatatgcta aaaaaggacg tttaacaaac tttgttagaa catgtatcga
     2221 aattttgtca tctcttcctt ctgttgtagt tggtctgttt ggttacctaa tcttcgttgt
     2281 tcaatttcaa tatggcttct caattatctc aggtgcctta gcacttactg tttttaactt
     2341 accccaaatg acacgtagtg ttgaagatag ccttcaaaat gtacaccaca cacagcgtga
     2401 agcaggattg gccctaggta tttctcgttg ggaaacggtc atttacgtcg ttgtccctga
     2461 agctctgcca agtattgtaa ctggtgttgt gctagcttca ggacgtattt tcggtgaggc
     2521 agcagcactt atttatacag ctggtcaatc tgctccagca cttgattggt caaattggaa
     2581 cgtccttagt gttacaagtc caatctcaat tttccgtcaa gcagaaactt tagctgttca
     2641 tatttggaaa gttaatagcg aagggacaat ccctgatgca acacaagtat ctgccggtag
     2701 cgccgcagtc ttattggttg ttattcttat tttcaacctt tcagctcgct cgatcggtaa
     2761 gaaacttcat tcgaaattaa cctcgtctaa ttaaaaggag tagtaatggc agaatataat
     2821 tgggacgaac gccatatcat taccttccct gaagaaaatt cagctctcac cacaaaagat
     2881 ttacacgttt actacggtga gaaagaagct atcaagggaa ttgatatgca atttgaaaaa
     2941 aataaaatta cagccttaat cggcccatct ggatgtggaa aatcaacata tcttcgtagc
     3001 ttaaatcgta tgaacgatac gattgatatt gcacgcgtga ctggacaaat catgtatgaa
     3061 ggaattgatg ttaacgctca agatatcaat gtttacgaga tgcgaaaaca tatcggaatg
     3121 gttttccaac gtccaaaccc gtttgcaaaa tcaatttata aaaacattac ctttgcatat
     3181 gagcgtgctg gtgtaaaaga taaaaaattc ttagatgaag ttgtcgaaac ctcactaaaa
     3241 caagctgctc tatgggatca agtaaaagat gaccttcata aatcagcatt cactttatct
     3301 ggcggtcaac agcagcgatt atgtattgca cgtgctatcg cagttaaacc agaaattctc
     3361 ttaatggatg aacctgcttc agcactcgat ccgattgcaa ctatgcaact agaagagaca
     3421 atgtttgaac tcaagaaaaa ttacactata attatcgtaa cacataatat gcaacaagca
     3481 gcacgtgcta gtgattatac tgcatttttc tatcttggag acttaatcga atacgataaa
     3541 acgaataaca ttttccaaaa tgctaaatgt caatctacta gcgattatgt ttctggtcga
     3601 tttggatagt gaggtaaaag atgacacaac ctattttaca agtcagtgat ttgtctgttt
     3661 actataataa gaaaaaagct ttaaaagaag tctcaatgga cttctatcct aatgaaatta
     3721 cagccttaat tggtccgtct ggttctggaa agtcaacact cttacgagct attaaccgta
     3781 tgggagactt aaatcctgaa gtcactctaa caggagcagt gatgtataac ggacataatg
     3841 tttatagccc gcgcactgat accgtagaac ttcgtaaaga aattgggatg gtttttcagc
     3901 agcctaatcc atttcctatg tcagtatttg aaaatgttgt ttatggtctt agactcaaag
     3961 gaataaaaga caaagctacg ttagatgaag ccgtagagac ttcattgaag ggcgcgtcta
     4021 tctgggacga ggtcaaagat agattacacg actctgctct tggtttatct ggtggtcaac
     4081 aacaacgtgt ttgtattgcc agaacccttg ctaccaagcc taaaattatc cttctagatg
     4141 agccaacctc tgcacttgac ccaatttctg ctggtaaaat tgaagagact ttacatggtc
     4201 taaaagatca atataccatg ttacttgtta cacgttctat gcagcaagcc tcacgtattt
     4261 ctgatcgtac aggtttcttc ttagacggta acttaattga atatggcaat acaaaagaaa
     4321 tgtttatgaa tcctaaacat aaagaaactg aagattacat tacaggtaaa ttcggttaat
     4381 accatataat aagatcagaa agagaataca atatgttaag atcaaaattt gatgaagaat
     4441 tagataaact gcataaccaa ttctatgcaa tgggaattga agctatcgga caaatcaaaa
     4501 aaacagttcg tgcttttgtc agtcatgatc gagagttagc taaagaagtg atagaagatg
     4561 atgtaacgct taacaacttt gaaacgaagt tagagaaaaa atcattggaa attattgcac
     4621 tccagcagcc tgtatcacaa gacttacgca ccgttattac cgttttaaaa gctactagtg
     4681 atgttgagcg tatgggagac catgcagcag cagttgcaaa agcaaccatc cgtatgaagg
     4741 gcgaggaaag aatcccagca gtagaattag aaatcaataa tatgggaaaa gcagttaaaa
     4801 acatgcttga agaagcctta acagcttata tcaatggtga tgatgaaaaa gcttacgaag
     4861 tagcagcaat ggatgaaata gttgatgatt acttccgtga tatccaaaaa atggtagttg
     4921 aaacaattca aaagcatcct gacgtagcat ttgccgccaa agaatacttc caagtattga
     4981 tgcacttgga gcgaattgga gattatggca aaaatatctg tgaatggatt gtttatttaa
     5041 aaactggaaa aattatcgag ttatgatata attattgctt gtagagtggt aattgtaagt
     5101 atagttcgtg cgatagaaaa gctgtaacta tcttgttaca gcttttctat cttttctagc
     5161 ccatacagga ctagtaatgt ttcaaatgtt tctgaattag ttaggagtta tatgaaaact
     5221 gtagaacatt ttgttaccaa atttgtccct gaaaactata accttttctt ggatattaat
     5281 cgtcaaacta aaaccttttc aggtaacgtt gctgtgtctg gcgaagcctt agataacaat
     5341 atctcttttc accaaaaagg gttaaccatt aaatctgtct tattagataa tcaaccatta
     5401 gatttccaac tagatgaaga caatgaagct atgcatattc aattgcatga aactggtagc
     5461 atggtattag tctttgaatt cagcggtcac attaccgata atatgacagg tatgtatcca
     5521 tcttactata cagttaatgg catcaaaaaa gaggttatct ccacacaatt tgaaagtcat
     5581 tttgctcgtg aagtattccc aagtattgac gaaccagaag caaaagcaac ctttgatttg
     5641 agcctaaaat tcgatcaaaa agaaggagag attgctcttt caaatatgcc ggaaatcaat
     5701 gctgaacaac gtcaagaaac aggtttatgg acatttgaca ccacacctaa aatgtcctca
     5761 tatttattag cttttgcctt gggggaattg catggtaaaa caacacatac caaaaacggc
     5821 actcttgttg gctcatatgc cacaaaagcc catcagttaa atgaattaga tttttcttta
     5881 gatatcgttg tgcgtgttat tgagttttat gaggactact ttggtgtacg ttatcctatt
     5941 cctcagtcac tccacgttgc tctacctgat ttttctgctg gagctatgga aaattgggga
     6001 ttagtaacat atcgtgaagt ctatctttta gtagatgaga actctagtgt ttccagccgt
     6061 cagcaagtag cattagttgt agcacatgaa atagctcacc agtggtttgg taacctcgtt
     6121 actatgaaat ggtgggatga tttatggtta aatgaatcat ttgctaatat gatggaatat
     6181 gtatcaattg attatattga accaaaactt aatatatttg aagactttca aacaggtggt
     6241 ctaccattag ctcttaagcg tgatgccaca gatggcgtcc aatctgttca cgttgaggtc
     6301 aatcatcctg atgaaattaa cacacttttt gatccagcta tcgtttatgc aaaaggtagc
     6361 cgcctcatgc acatgttacg tcgctggtta ggcgatacag attttgccgc aggactaaaa
     6421 atttattttg aaaaacatca atatcaaaat actattggtc gtgacctttg gaatgcactt
     6481 tctcaaacct caggaaaaga tgttgcagct ttcatggatt cttggttaga gcaaccagga
     6541 taccctgtga tggcagccaa aatagaagaa gatgagctca ttttaactca aaaacaattc
     6601 tttattggtg aacacgaaga taagtctcgc ttgtggcaaa ttcctcttaa tagtaattgg
     6661 gaaggaatcc cagagatatt aaccgaggaa acggtagtaa tccccaattt tagtcagtta
     6721 gctgaaaaga acaaagaaaa tggtgcactg cgtttcaata ctgaaaatac tgcacattac
     6781 attactaact atcaaggtca actcttagag catatcattt ctgatcttcc tcttatggat
     6841 aacatttcta aactacaaat tgtacaagaa cgtcatttac ttgctgaaag tggtatgatt
     6901 tcatatagta gtttaatacc tttagtctct cttttgagcc aagagacatc atatcttgtt
     6961 aactctgcta ttaagagtgt tattgatggt ttatccttat tcgtccagga agatagccaa
     7021 gatgaattcg actttaaaga atttgttaat aaattatcag catttaattt taatagactt
     7081 ggctttgaaa aaagagaagg agaaggagat gatagcgaaa tggtccgtca tctatcccta
     7141 agccttgctc tctactcaga taatgaacat gctattgaag aagctcacca tatttttaaa
     7201 gcccacgaaa ataatatcgc tgctatacca gctgcaattc gcctgttagt tcttaccaat
     7261 gaaatgaagc atttcgaatc caaagaactt agccacttgt tattagagac ttatagtact
     7321 actaccgatg ggaatttcaa acgacaacta gcgagtgctt tatctcatac tacagatagc
     7381 aagacactca agaaattatt gtcagattgg aaaaataaag atattgtgaa accacaagac
     7441 ttagctatga gttggtatgc tactttcctt aaaaattctt ttacacaaga aagtgtctgg
     7501 gagtgggccc aagaaaactg ggaatggatc aaagcaactc taggtggtga tatgagtttt
     7561 gataaatttg tgatttatcc ctcaagtagt ttcaaaacag aagaacgcct tgaacaatat
     7621 aaaaactttt tcgagcctca attaagcgat atggcgatta gccgtaatat ttcaatggga
     7681 attaaagaaa tttctgctcg tgtcctttta atcacgaagc aaaaagaaga agttatcaat
     7741 actatcaaaa aatatatcta ataaactcaa atagagattg atggattatt caatctctat
     7801 ttctttgtct gtgtcatgaa attagttgac ataaaacata ctttatttag gatttattaa
     7861 gcaaagtttg gtattctaat attaatagtt cagataaaat atgtacatcg aaaggatata
     7921 ctatgataaa aattttatta attgaagatg acttgagtct ttccaattcc gtttttgatt
     7981 tccttgatga ctttgccgat gtcatgcaaa tttttgatgg agaagagggt ctttacgaag
     8041 ccgaaagtgg agtctatgat ctgattcttc tagatctcat gttacctgaa aaaaacggct
     8101 tccaagtgtt gaaagagcta cgtgaaaaag gtattacaac ccctgtatta ataatgaccg
     8161 ctaaagaaag cattgatgat aagggacaag gtttcgactt aggtgctgat gattatctaa
     8221 caaaaccttt ttacctagaa gaactaaaaa tgcgaattca ggctctttta aagcgttctg
     8281 gaaaatttaa tgataacagc ctaatttatg gtgatattcg cgtagacatg tctactaact
     8341 caacctttgt taatcaaaca gaagttgagc tcttaggtaa agagtttgat cttttagtct
     8401 atttccttca aaatcaaaat gttatcttac cgaaatctca aatctttgat cgtatttggg
     8461 gatttgatag tgatacaacg atttcagttg tagaagtcta tgtctctaag gttcggaaaa
     8521 aattaaaagg aacacttttt tcagaaaacc ttcaaacttt gcgtagtgta ggatatattt
     8581 taaagcatgt cgaaaccgat taagataagt aaattcaaaa aaaatgtctc agatagtcat
     8641 tttatccatt ttttcacagt ttttagtggt atttttctgg ttatgactgt tatcatcctc
     8701 caagtcatgc gctatggtgt ttattcttct gttgatagta gtttaaagta tatcagtacc
     8761 catcctaaaa attacatcaa tatggttatg tcacgtactg ctgcctacct agataactcc
     8821 aatatcgcaa gtgtcaagtt aaaacccggc ggtcagacag tagctaatac tgatattatt
     8881 ctttttacat cagaagaaga agtcattaac tattttgacg cattttcaaa ctatcagttt
     8941 ttaaaaccta ataaaaaaaa tctaggtggc attagtgaat taactttaac caacatcttt
     9001 ggtcaagatg agacatatca tgctgtcacc gtaaaagtta ataaccctgc ttatcctaat
     9061 gtgacttaca tgacagctat tgttaatatt gatcaattag taaatgctaa agaacgctat
     9121 gaaaaaatta ttatatttgt catgactact ttttggatta tttcaattgg agcaagtatc
     9181 tacttagcta aatgggcaca aaaaccgatt atagaaaact acgaaagaca gaaggctttt
     9241 gtcgaaaatg ctagccacga gctgcgtact cccctagctg tcttacaaaa tcgcctagaa
     9301 acgctattca gaaaaccgaa tgccaccatt ttagaaaata gtgaaaatat cgcttcaagt
     9361 ctagatgaag tacgaaatat gcgtatttta accactaatc ttttaaactt agcaagacgt
     9421 gatgatggaa tcaaaccaga attagctgtt attaagccta cactttttga tagtatcttt
     9481 gagaattatg atctaatcgc ccaagagaat ggtaaaaatt ttactggaca taatatgatc
     9541 caagatagtt tcaaaacaga taagacactg ctaaaacaac tcatgactat tttatttgat
     9601 aatgctataa aatatactga caatgatggt agcattgact ttaccatatc tgaaactgat
     9661 aagtatctct ttttagaaat cgctgataat ggaccaggaa tttcagagga ggataaagtc
     9721 agaatttttg atcgttttta ccgtgttgac aaagcaagaa cacgacaaca aggagggttt
     9781 ggcctcggcc tttctttagc tcaacaaatc gttaactcac tgcgaggtaa tattacagta
     9841 attgataata aacctagagg cagtattttt aagataaaac taccaaaagt aaaaggttag
     9901 gccatgttag tcctaacctt ttaaatttta aagtggtctt gcatacttga tatagcaagc
     9961 tgataggaaa tttggtataa taaaagctat ggaaattgaa aaaacaaatc gtatgaatgc
    10021 cctttttgag ttttatgcgg cattattaac agataaacaa atgaattata tcgagttata
    10081 ctacgccgat gactacagcc ttgctgaaat agctgaagaa tccggtgtta gccgacaagc
    10141 tgtctatgat aatatcaaac gaactgaaaa aattcttgag gcgtacgaaa tgaaactaca
    10201 tatgtattca gattatattg tccgtagtca gatttttgat gacattctag aaaaatatac
    10261 cgatgatgcc tttttacaag aaaaaatttc cattctaagt agcattgata atagagatta
    10321 aatgaggaga aataatttat ggctttcgaa agtttaacag agcgcctgca aggagttttt
    10381 aaaaatattc gtggtaagaa aaagttatct gaaaaagatg tgcaagaagt caccaaggag
    10441 attcgtctag ctcttttaga agctgacgtt gctcttccag ttgttaaaac ctttattaaa
    10501 catgttcgtg aacgtgctgt tggacatgaa atcattgata cactagatcc tacgcaacaa
    10561 attgttaaga ttgtcaatga agagctaaca gatttattag gggctgagac ctctgaaatt
    10621 gaaaaatccc ctaaaattcc aaccatcatt atgatggttg gtctacaagg tgctggtaaa
    10681 acaacttttg ccggtaagtt agcaaacaaa ctaatcaaag aagacaatgc tcgtcctatg
    10741 atgatagcag ctgatattta tcgaccagct gctattgatc agttaaaaac ccttggtagc
    10801 cagattaatg ttcctgtctt tgatatggga actaaccatt cagcagttga gattgtcact
    10861 aaaggtttag aacaggctcg tgaaaatcgt aatgactatg ttcttattga tacagccgga
    10921 cgcctacaaa ttgatgcaac ccttatgcag gaattgcatg atgtcaaagc catagctcaa
    10981 cctaatgaaa tactccttgt cgttgacagc atgattggac aggaagctgc aaatgtcgct
    11041 gaagaattta accgtcagct cagcattagt ggtgttgttc tgacaaagat tgacggtgat
    11101 acacgcggtg gtgctgccct ttcagtacgc gaaattactg gaaaaccaat taaatttaca
    11161 gggacagggg aaaaaatcac tgatattgaa actttccatc ccgatcgtat ggcaagccgt
    11221 atattaggga tgggagacct gctaacactt attgagcgcg ctagtcaaga atacgacgaa
    11281 aaacgttcaa tggaattagc ggagaaaatg cgtgaaaaca cctttgactt caacgatttt
    11341 attgatcaat tagatcaagt tcaaaatatg gggccaatgg aagatctcct taaaatgctt
    11401 ccaggaatgg ctaacaatcc agcaatgaaa aacttcaaag tggatgaaaa cgaaattgct
    11461 cgcaaaagag ccattgtgtc atcaatgact ccagaagaga gagaaaatcc aaatctcctc
    11521 aatccaagtc gtagacgtcg tattgctgct ggatcaggta atactttcgt tgatgttaat
    11581 aaattcatta aagattttaa ccaggcaaaa caaatgatgc aaggcgtgat gtccggtgat
    11641 atgaataaaa tgatgaaaaa aatgggaatt gatcctaata acctccctaa agatatgcct
    11701 ggaatggacg gaatggatat gtctaattta gaaggtatga tggggcaaaa tggcatgcct
    11761 gacttatcta gtttaggtgg cgatatggac ttcagccaaa tgtttggtgg tggcttaaaa
    11821 ggaaaagtag gagcctttgc agctaaacaa tcaatgaaac gtatggctaa taaaatgaag
    11881 aaagctaaga agaaacgcaa ataaaaatgt ctatttaaat agacattttt atttttgtct
    11941 gctataatag acaaaaaggt ggtgatattt atgtatttag cattaatcgg tgatatcatt
    12001 aattcaaaac agatacttga acgtgaaact ttccaacagt cttttcagca actaatgacc
    12061 gaactatctg atgtatatgg tgaagagctg atttctccat tcactattac agctggtgat
    12121 gaatttcaag ctttattgaa acaatcaaaa aaggtatttc aaattattga ccatattcaa
    12181 ctagctctaa aacctgttaa tgtaaggttc ggcctcggta caggaaacat tataacatcc
    12241 atcaattcaa atgaaagtat cggtgctgat ggtcctgcct actggcatgc tcgctcagct
    12301 attaatcata tacatgataa aaatgattat ggaacagttc aagtagctat ttgccttgat
    12361 gatgaagacc aaaaccttga attaacacta aatagtctca tttcagctgg tgattttatc
    12421 aagtcaaaat ggactacaaa ccattttcaa atgcttgagc acttaatact tcaagataat
    12481 tatcaagaac aatttcaaca tcaaaagtta gcccaactgg aaaatattga acctagtgcg
    12541 ctgactaaac gccttaaagc aagcggtctg aagatttact taagaacgag aacacaggca
    12601 gccgatctat tagttaaaag ttgcactcaa actaaagggg gaagctatga tttctgattt
    12661 tttaagggat aatccaatcc taactttatt attttgcgca cattttttag ctgactttca
    12721 gtggcaaagt caatccttag cagatagtaa atcacatagt tggaggggac tctggagaca
    12781 tctcttgatt gttttcctcc ccctagcagc tttaatgata ttgataccag aaactacttt
    12841 gttaaattta agcatatggg gaagtcatat tgttattgat agtataaaga aattgagtta
    12901 cccttgggta gaggaagggc atttccaaaa agcagctttt attatagacc aactagccca
    12961 ctatacttgc attatcgttt tttaccatgc gcttcctaca tatttaccgc ctaatcattg
    13021 gcttttgcct atcaagcact ttattgtcat agctctggtt tttatcatta tcaccaaacc
    13081 cattaatatt gtgtttaaaa ttttctttaa taaatttcaa gctaaagaat tatcatcatt
    13141 attaacacaa gaaaaaacaa aaataatgaa agaaaaatct gaagatcatg aagaaacaat
    13201 tgaaggagca ggagctatga ttggtaattt agagcgtcta attatggcta tattactaat
    13261 ctcaggacag tatgctgcta taggtcttgt ttttactgca aaatccattg cacgttacga
    13321 taaaatttct aaaagtcagg tttttgccga atattattta attggatcat tgttctcaat
    13381 tataagtgtt ttgataactc attgggtact tttggtatag aaaagcgcta gaaatatcct
    13441 aacgcttttc ttttattctg tcttataagc ataagtaaaa tcaacattca aaccagttga
    13501 atttcttacc aaccccttta atttagaatt ttggagtcct tttttcccta ggtaataaac
    13561 tgggttataa tatgattgat caaataaagc tttttcagca gttttgtaat cattagcggc
    13621 atctcctggt ttcaaggcgt cagtagtaat tgccttttga taggcattgt catactcttt
    13681 agaagaaaat ttaccataat tatacgcaga attagtcgtg aataacccat agaaagttga
    13741 tccttctgga taatctcctc cccatgagaa gagcacgaca tcaaaatttt cattttttgc
    13801 atcttcaagg cgttgcttga aagtaacaaa tttttcttca acagttagac caggcaaagc
    13861 actctcccat gtagatttca catagtcaac agcattttta gctgctggag agtccgaatc
    13921 agctgtgatt gttaacttga gagatgactg accaacctca gcaagacctt ctttaaaaag
    13981 tttttgagct ttacttgtat tatacgtata acctggggca gtatacttgg aaagatcctc
    14041 accattaggt aatttggcta attttttagg gaccagagat tcagcaggag ttgaacctgt
    14101 atcaactgcc gctttaacaa cacctttacg atcagtcgct aaattcaaag cttgcctaat
    14161 tttctgattt gtcaatgctt taaccgaacc agtttggtta taaatagcag aagttcctga
    14221 tatttcagca aaatcaatct gaccttgctt gtacatttga actgcagtat ctggtttttt
    14281 gatagtttga actatgacac tatttgtttt aacatgttta gaatcccaat aatatttatt
    14341 tttgactaat ttaaatttac catttgaacc attccaatcc ttaacaaggt aaggacctga
    14401 gtaaatttga ttttttgatg ttgtaccata atctttacca actttttcaa catacgattg
    14461 tttttgaggc ataaaattag agaaggctaa ataatattta aattgagggc atggacttgt
    14521 caatttaaag gtcacttgat tgcctttagc tgttacacca agtttattta aatctttaat
    14581 atctccagaa ttaatcttat cagcatttaa aaggtgcgat tctgtcgcca agtaagcata
    14641 ttcagatgca gttttaggat caactattct ccgccatgtg taaacaaaat cttcagcgct
    14701 tagtttgctt ccatctgacc attttaaatt atcacgaagt gtagcagtat aagtaagacc
    14761 atcagaagaa acggatactt ttttagctaa atcgggtttt ggtttgccct ctttatcaat
    14821 tctaagtaaa ttgctgcctg aattacctat agctaagttt gaataagcat ctgtattctt
    14881 tgaaatgtcc aatgtactga tttcagtagg aatataccaa ttaataactt tattatctcc
    14941 agagttcttt gactcattac cacaggcaac cagggtaact gtggtggctg ttagagtcgc
    15001 caaagatata cccaaaaact taagtttttt tctattaaaa ctagacataa taccagtcct
    15061 cc
//
LOCUS       DATRSG010000054        14809 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRSG010000054 DATRSG010000000
VERSION     DATRSG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14809
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.054"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            5..1159
                     /locus_tag="VBD89_001554"
     CDS             5..1159
                     /locus_tag="VBD89_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7136072.1"
                     /translation="MFFKWILNNQAVIALMITFLVFLTIFIFTKISFMFKPVFDFLAV
                     LILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLLIWAMSSFIPMMSNQ
                     LRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNISSQAVSYAEKFSKNV
                     LDWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLPTKLRQPADRILREM
                     NSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIAGVLNMVPYLGSFVAQIP
                     VFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHPITIMFILLTSGAMF
                     GVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEERSEEVKNVE"
     gene            1149..2375
                     /locus_tag="VBD89_001555"
     CDS             1149..2375
                     /locus_tag="VBD89_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136073.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2485..4167
                     /gene="alsS"
                     /locus_tag="VBD89_001556"
     CDS             2485..4167
                     /gene="alsS"
                     /locus_tag="VBD89_001556"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO7136074.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4181..4900
                     /gene="budA"
                     /locus_tag="VBD89_001557"
     CDS             4181..4900
                     /gene="budA"
                     /locus_tag="VBD89_001557"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO7136075.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(4954..6609)
                     /locus_tag="VBD89_001558"
     CDS             complement(4954..6609)
                     /locus_tag="VBD89_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO7136076.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            6968..7972
                     /locus_tag="VBD89_001559"
     CDS             6968..7972
                     /locus_tag="VBD89_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7136077.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            7985..8848
                     /locus_tag="VBD89_001560"
     CDS             7985..8848
                     /locus_tag="VBD89_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136078.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            8848..9609
                     /locus_tag="VBD89_001561"
     CDS             8848..9609
                     /locus_tag="VBD89_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7136079.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            9906..11567
                     /locus_tag="VBD89_001562"
     CDS             9906..11567
                     /locus_tag="VBD89_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7136080.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            11624..12412
                     /locus_tag="VBD89_001563"
     CDS             11624..12412
                     /locus_tag="VBD89_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO7136081.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            12502..13212
                     /locus_tag="VBD89_001564"
     CDS             12502..13212
                     /locus_tag="VBD89_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO7136082.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLRNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            13450..14121
                     /gene="rpiA"
                     /locus_tag="VBD89_001565"
     CDS             13450..14121
                     /gene="rpiA"
                     /locus_tag="VBD89_001565"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO7136083.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            14178..>14809
                     /locus_tag="VBD89_001566"
     CDS             14178..>14809
                     /locus_tag="VBD89_001566"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7136084.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVG"
BASE COUNT         4731 a         2288 c         3046 g         4744 t
ORIGIN      
        1 tagtttgttt tttaaatgga tcttaaataa tcaagcagtt attgctctca tgattacctt
       61 tttggtattt ttaacgattt ttatttttac caaaatctct tttatgttta aacctgtgtt
      121 tgattttctt gctgtgctga tattgccgct tgtaatttct ggcttgcttt attacctatt
      181 aaaacctatg gttacatttt tagagaagcg gggaattaag cgtgtaacag cgatattatc
      241 agtttttact attataatcc ttctgttaat ttgggcaatg tctagtttta ttcccatgat
      301 gagtaatcaa ttacgccatt ttatggaaga tctcccttca tatgtgaata aagtgcaaat
      361 ggaaacaagt tcgtttatag atcacaaccc ttggttaaaa tcttataaag gggaaatatc
      421 gagcatgtta tctaatatca gtagccaagc ggtctcttat gctgaaaaat tttcaaagaa
      481 tgttttagat tgggcaggaa atttagctag tacagttgca cgtgtgacag tagcaacaat
      541 catggctccc tttattttgt tttatctttt aagagatagt cgcaacatga agaatggttt
      601 cttaatggtt ttaccaacca aactacgcca accagctgat cgtattttgc gagaaatgaa
      661 tagtcaaatg tcaggatatg tgcaaggaca aatcattgtt gctattactg ttggtgttat
      721 tttttcaata atgtatagta ttataggcct tagatatggc gtgacattag ggattattgc
      781 cggtgtgtta aatatggttc cctatttggg aagttttgtc gcccaaattc cagtgtttat
      841 cttagcgctt gtcgcaggac ctgttatggt tgttaaagtt gcgattgttt ttgttattga
      901 gcaaactcta gagggacgct ttgtctcacc tttggtttta ggtaataaac ttagcattca
      961 tccaattaca attatgttta ttttattaac ctctggagcg atgtttggtg tttggggagt
     1021 attcctcagt attccgattt atgcatctat caaagttgtt gttaaagaat tgtttgattg
     1081 gtacaaagct gtcagtgggc tatatacaat agatgttgtt actgaagaaa gaagtgaaga
     1141 agttaaaaat gttgaatagt gaaaaaatga ttgtttctat ccaaaatcag gatttagaac
     1201 atgctaacaa gtatttcgaa aaagcgttga aaaatgatcc tgaggaaatc ttattggaac
     1261 taggagctta tttggagagt attggttttt taccccaagc aaaacgtctc tatgatcaaa
     1321 tccgaccaaa ttatccagaa gttgctatca atttagctca aattgttgcc gaagatggag
     1381 atattgaaca agcattctta tacttagatt atatctcaga agatagtcaa gagtatgttt
     1441 cagccctcct cgttatggct gacttatatg atatggaagg cttgactgat gttgcgcgtg
     1501 aaaagctttt gctagccagc aaacttagcg atgacccatt ggttacattt ggtttagctg
     1561 aaatgaacct tagtttggaa cattatcaag aagcgataga aggatatgca tcacttgaca
     1621 atcgagaaat cttagaaacg actggagttt caacttatca gcgtattggt aaatcctacg
     1681 ctattatagg gaaatttgat gcagctatcg aatttttaga aaaagccgtt gacattgagt
     1741 atgatgattt aacagttttt gagttagcaa caatccttta tgatcaagaa gaatatcaaa
     1801 aagctaacct ttattttaaa cagttggata ctattaatcc agactttgat ggctatgaat
     1861 atatctatgg tttatctctt cgtgaggagc ataaatctga agaagcttta cgtctagttc
     1921 agcaaggtct tcgtaaaaat agttttgatg gtcaacttct cttactagct tcacaattat
     1981 cgtatgaatt acatgatgtt ccttcgtcgg agtcttattt gaaacaagct gaaaaagttt
     2041 ctgaaaacca agatgaaatt gttatgagat tgtcaaattt gtatcttgaa gaagagcggt
     2101 ttgaggaagt cttggaatta gacaatgatg atttagaaaa tatccttgca aaatggaata
     2161 tcgctaaagc acacaaggct ttagaaatgg atgatagtgt agactattat caatctttgt
     2221 ataatgattt aaaagataat ccagaatttt tacaagatta tgcttacatt ttgagagaat
     2281 ttggttacct tgataaggca caagaagtag gaaaggctta tttaaaatta gttccagacg
     2341 atatagagat gtcagaatgg gtgaataaca tttaaatagg aaaacactta cagaaaaaaa
     2401 taaaaaattc acaagtaaat tttcacaatc tgatgtaaaa actgtgatat aattgaacca
     2461 ttcttgtgaa aagagggaga taaaatgaca gaatcacata atcaatatgg ggctgactta
     2521 attgtcgata gcctcattaa tcatgatgtc aaatatgttt ttggtatccc aggagctaag
     2581 attgaccgcg tatttgacac tctagaagat aaaggtccag agttaattgt tgctcgtcat
     2641 gagcagaacg cgacttttat ggctcaggct gtcgggcgta ttacgggaga accaggagtt
     2701 gttattgcaa ctagtggacc tgggatctcg aacttggcaa cagggttagt taccgcgaca
     2761 gatgaaggtg atgctgttct tgcaattggt ggacaggtta agcgaggaga tttattgaaa
     2821 cgtgctcatc aatcaatgaa taacgttgct atgctagaac ctattacaaa atattctgct
     2881 gaggttcatg accctaatac tttgtcagaa actgttgcaa acgcctatcg tcttgctaaa
     2941 tctggtaaac caggagcaag ttttatctct attcctcaag acgttacaga cagtccagtc
     3001 tcagtaaaag caatcaaacc tcttagtgca cctaaattgg gttcggcatc tgttttagat
     3061 attaattatt tagcgcaagc tattaacaat gctgttttac cggtactgtt attaggaaat
     3121 ggcgcatctt cagagggagt aactgctgcc gtacgtcgac ttcttgatgc tgttaaacta
     3181 ccagtagtag aaactttcca aggtgcgggt attgtgtcac gtgagctcga ggatgaaaca
     3241 ttctttggtc gtgtaggcct cttccgtaat caacctggag atatgctttt gaagcgtgca
     3301 gacctagtta ttgctatcgg gtatgaccct attgaatatg aggcgcgtaa ctggaatgct
     3361 gaaatttctg ctcgtattat cgttattgac gttgaacaag cagaaattga tacgtatttc
     3421 caacccgagc gagaactgat tggggacatg gcacatacac ttgatttact tttaccagct
     3481 attaaaggat atgaattgcc agaaggttca aaagagtatt tgaaaggtct tcgtaataac
     3541 attgaaaacg ttagcgatgt taaatttgat cgtgactcag ctcatggact tgttcatcct
     3601 cttgatctca ttgatgtact ccaagataat accaccgatg acatgactgt tacagttgat
     3661 gtgggaagcc attatatttg gatggctcgc tactttaaat catatgaagc gcgtcacttg
     3721 ctcttctcaa acggaatgca aacattagga gttgctctac cttgggcaat ttctgcggct
     3781 cttttgcgtc ccaacacaaa agttatttca gtttctggtg atggtggctt cttattctca
     3841 gctcaagagt tagaaactgc agttcgtctt catctaccaa ttgttcacat tatttggaac
     3901 gatggaaaat ataacatggt tgaattccag gaagaaatga agtatgggcg ttcttcaggt
     3961 gttgactttg gaccagttga ttttgtaaaa tacgctgaaa gtttcggtgc taaaggttac
     4021 cgtgtagaca gcaaagacag ttttgaagaa acactcaaac aagctctaat cgatgctgaa
     4081 aatggaccag tgttgattga tgtgcctatt gactataaag ataacgtgac actaggtgaa
     4141 acaatcttac ctgacgagtt ctactaagac gaggtctaat atgtcagaaa cagttaaatt
     4201 atttcaatac agcaccttga gttcactgat ggcaggattg tacaaaggca gtctaactat
     4261 cggagaatta ttaactcacg gtgacttagg aatcggtact gttcatatga ttgatggtga
     4321 attgattgta ttggatggga aagcttatca agctattggt acagatggta aagcagagat
     4381 tatccaatta tcagatgatg tgacagtacc ctatgcagcg gttttaccac accatatcca
     4441 aaaacaattt gatatcaatg ctgagacaga taataaggat ttggaagaga tgattctaaa
     4501 aaactttgaa ggtcaaaatc tctttaagtc tttaaaaatc aaaggaacgt tttcaagaat
     4561 gcacgttcgt atgattccaa aatctccaca acataaacgt tttgcagata ttgcatctaa
     4621 tcagccagaa tttaccagag agaatgtatc tggtacgttg gttgggattt ggacgccgga
     4681 attatttcat ggtgtggggg taaaagggtt ccatgtccac tttatttcag acgatttaac
     4741 tttcggtggg catgttatgg attactcgct cacacaagga aaagttgaaa ttggaaaggt
     4801 tgaccaactt gaccaatgtt tcccaactca agatcaagaa tttttgaaag ctaactttga
     4861 cctacaaaag cttagagaag atattgattt aagtgaataa gaatacctat aaataattaa
     4921 taacactaaa aatagacagg gatatgtccc agtctatttt tttagtttaa ggctatctat
     4981 tttttcttgt gtgggagtga ctcgtaaggt tttttgacca gtgtaagtta caaaacctgg
     5041 cttagtacca cttggtttat tgagtttttt tgcttcaatc atgtctactt gaacaagatt
     5101 ggagagacga gctttagagt aataagcagc taactcggcg gcatctgtct taacctcgtc
     5161 acttggattg aggttatccc gaattaacac atggcttccg gggatatcct tggcatgaaa
     5221 ccacaactcc cctttacgag ccattttaaa tgttaactcg tcattttgga gattattacg
     5281 tcccaccata ataatagtct tgccatcgga tgctaaatat tgttcaggtt ttttacgctt
     5341 atgttgttta tcatgtgcgc gtcgcttaat aaatccagtt tcaacaagtt cttcacgaat
     5401 atcattgata tcttccatag aagcatgatt tagcgatgtt tcaacagatt caaggtaggt
     5461 gattgtattt ttcgtatctg aaataattcc ttttaaatgt tttacagctt cttttaattt
     5521 ttgatatttt ttaaagtagc gttgggcatt ttggttgggt gttaaagctc gatcaagtga
     5581 aatttcaatc gtttggttgg tgtaataatt atcaagaaca acgatatctt gattatttgg
     5641 caccatagag agataggtag ttagaagctc tcctttttgg cgaaactcct ctgcattttc
     5701 agtagctaga agttcgtctt gttgcttagc tagttttttg atatttttct ctaactcact
     5761 ttgaacacgg tgaatgaggt cactagcttg ctgtgctatg cgatcttttt cagccttctc
     5821 ttggtagtaa taatctaaca attgtccaag agactgaaat gtttcgtgac tgcttgagaa
     5881 ttggacagcg gaaaacgatt tttctgttag gctaggataa tattctctcg aaaagaattg
     5941 acgaaaatcg ttaagtttat tgtctttcaa acagtgagat aattctagcg cagtatcgcg
     6001 tcctaaacct tggaagagct gttggaggtt tttagggctt aggtcattgg tttgaagcag
     6061 ctcaaataga gtttgatcag aaatatcaaa aggattgatt gcttttgttt tgggtggggc
     6121 aatgtaagtt gaaccaggaa ggatagtacg gtaggagttt tgcgagaaac caacatgctt
     6181 aattgattca ataattttat gttcattttt atcaatcaag ataatgttac tgtgttttcc
     6241 catgatttca acaacaagtg tcgctttaat gtgatcaccg atttcgtttt tattagacac
     6301 gatgatttct aatatacggt cattttcaat ttgttgaatg gtttcaatca ctgcaccttg
     6361 caaatacttt ctcatgatca ttgtaaaagt atttggattt tgaggatttt ggaaatttgc
     6421 ttcggtagtt tggatacgtc caaatacagg gtgagcagat aggagtagtt tatagttgcg
     6481 acgattattg cgtattgtta ataccaattc atggtcaaaa ggttggttaa ctttttggat
     6541 acgtcctttt tcaatctgtt cttgtaattc atttgtgagg tgatgtaaaa aaaatccatc
     6601 aaaagacatg gcgtccccct tattctaaaa attatagctt atttattata ccataggctg
     6661 agctaaaaat atttttttat aacgcaaaag gcttgcaatt ttcagaatat agtatataat
     6721 tatagaaaaa ttcatgagaa aggatgacta gtaaatgact aaaacattat ttaccaattg
     6781 gcaaaactgg cgtagataaa gttgtgttta tgggtgcata gatacgactt tttggagtac
     6841 ttcaaaaaag tatctatgcg ctggtctagt ctaacaatag agagcgcaag gtaagttatt
     6901 tatcaggcgc tttttctttt gtgctaaact ccagaaagtc aggatattat taaagtgagg
     6961 agaaaatatg aaaaataaag gactgatagc tacgctaatt ctattgacca tattagtggt
     7021 tggggaatta ttttataaca aatcagaaaa acatcttaat ttatcagaaa agcaagttgt
     7081 taaaattggt attttacagt atgtaaccca tgatgctttg gatgctattg aaaagggagt
     7141 tgaagatggt ctagctcaag aaggatataa agggaaaaaa gttaaattaa ccgttttaaa
     7201 cgccgaagct gatcaaagta aaattcaagc gatgagtaaa caattagtta atcatcacaa
     7261 tgatattttg attggaattg cgacaccatc agcacaaggt ttagcagctt cgactaagga
     7321 tactcctata atcatgggag ctgtttctga tcctttaggg gccaagttag taacgaatat
     7381 gaagaaacca acaactaatg ttacaggatt atcaaacgtt gttccgacca agcaaactgt
     7441 gcaattaatc aaggatataa ctcccaatgt taaacgtata ggaattcttt acgctagtag
     7501 cgaagataat tctgtgtcac aagtaactga gtttacaaaa tatgctcaaa aagcagggct
     7561 agaagttctt aaatattctg taccatctac aaatgaaatt aaaacaagta tgtcagtcat
     7621 gaccaaaaaa gtagacgctg tctttgttcc acaagataat actattgcga gtgcttttag
     7681 aacagttatc gtagcagcaa accaagctaa tatacctgtg tattccagtg ttgataccat
     7741 ggttgaacaa ggaagtatag catcagtagc gcagagtcaa tatggcttag ggttagaaac
     7801 tgcaaaacag gccattaaag tcctaagagg gaagccggtt aaagatgtac cagtcaaagt
     7861 tattgataca gggaagccaa gcctcaattt gaaagcagcc aaacacttag gaattaaaat
     7921 tcctaagaag attatgaaac aggcagaaat taccgttaag gttgacgatt agaaagaggg
     7981 ttttatgatt atttcatcag tttcacaagg tttattatgg gggatattag gattagggat
     8041 atacttaacc tttagaatct taaaatttcc agacatgaca acagagggct ccttccctct
     8101 agggggagcg gtttgtgtta ccttaatgaa tcaaggggtt aatcctattt tggcgacgat
     8161 tctaggcatg ctatcaggga tgttagcggg atttgttact ggtttacttt atactaaagg
     8221 aaagataccg acgattttag cagggatttt agtaatgacc tcatgccact ctattatgct
     8281 aatggttatg aaaagagcaa atctaggttt aaatgagatt caaacattga aagattttct
     8341 tccgttttca aatgatttaa atcttctcgt attaggtttg atagctattc tcttggcgat
     8401 ttctgtctta atttattttc tctatacacg cttggggcaa gcttatattg caacaggaga
     8461 caaccctgat atggcaaaga gcttcggcat tgataccgac aaaatggaaa tgcttggctt
     8521 aattgtatca aatggcttaa ttgctttatc aggagcttta gtaagtcaac aagatggata
     8581 tgctgatgta tcaaaaggta taggtgttat tgttattggg ttagctagta ttattattgg
     8641 agaggtactt tactctactg gtttaactct ctttgaacgt ttaattgcta tagtggtggg
     8701 ttctattctt tatcaatttt taataacggc agtaatcgcc ttaggattta atacgaatta
     8761 cctaaaatta tttagtgcaa tagtgcttgg aatttgttta atggttcctg tcctaaaaac
     8821 gaagattttg aaaggagtac gcttataatg atgatgaaaa tcatagagtt aaaagaagca
     8881 accgtacaag tcagtaatgg tttagcagaa atgaaaacga tattagatca tgttaatttg
     8941 agtatttatg aacatgactt cattacaata ttgggtggaa atggagctgg aaaatcaacg
     9001 ctttttaatg taattgcagg taccttgatg ttaagcagtg gaaatattta catcatggga
     9061 caagatgtta ctaatttatc agcagaaaaa cgagctaaat atttatcacg ggtatttcaa
     9121 gatccgaaaa tgggaacagc acctaggatg acagttgctg aaaatttatt agttgctaaa
     9181 tttcgaggtg aaaagagacc gttagttcct agaaagatta ctaactatac cgaagaattt
     9241 caaaaattga ttgctagaac tggtaatgga cttgatcgcc atttagagac acctacaggt
     9301 ttattatcag gtggacaaag acaagcgctt agtttattaa tggcaacttt gaaaaaacca
     9361 aatctgttat tattggacga acacacagca gcgcttgatc ctagaacaag tgtttcgctt
     9421 atgggcttga ctgatgagtt tatcaaacaa gattcgttga ctgccttaat gattacacat
     9481 catatggaag atgctctcaa atatggaaat cgtgtattag taatgaaaga tggcaagatt
     9541 gtcagagatt taaatcaggc acaaaaaaac aagatggcta tagctgacta ttatcaatta
     9601 tttgattaaa aagaagtgaa atggaagtgt ttcacttctt tttctataca ctttgcaaga
     9661 aattattttc aaaatatgat atactaataa tgctgtaaat cgacttgtag agacaaggac
     9721 cgtaagtaca aatggaatga aaataaggac acactcttta attcatattc ctattattga
     9781 ttaataaggt ttttcttcaa acccaagaaa agttaccgat aaatcaatta ctaatgataa
     9841 taattggaca atttcttgtc tgctttataa aaaagaatta acaaaattta aggagaaaac
     9901 tcattatgag cgacataaaa atcattgctc ttggaggtgt tcgtgaaaac ggaaaaaacc
     9961 tatatgtagt agaagtcaat gattctatct ttgttttgga tgctggactc aaatatcctg
    10021 aaaatgaaca actgggtgtt gatgttgtta ttccaaatct tgattactta attgaaaata
    10081 aaaaacgtgt tcaggggatt ttcttaaccc atggacatgc ggatgctatt ggtgcattgc
    10141 cttatattat cgccgaagtc aaagcacctg tgtttggttc acctctcaca attgaattag
    10201 ctaagttatt cgttaaaaat agtacagcag tcaagaaatt caataatttc catgtgattg
    10261 atagtgaaac ggaaattgaa tttcaagatg cggtgatttc gttctttaaa acgactcatt
    10321 caattccaga gtcaatggga attgttattg ggacaaaaga gggaaatatc gtttacacag
    10381 gtgactttaa atttgatcaa gctgcaagga aatattacca aacagatctg gcgcgtttag
    10441 ctgaaattgg tcgtgatggt gttttagcac ttttatcaga ttctgcaaat gctacaagca
    10501 atgagcaagt tgctagcgaa tatgaggtcg gtgatgaaat taagagcgtt attgaagacg
    10561 cagaaggtag agtcatcgta gcggcagttg caagtaatct cattcgtatt caacaagtct
    10621 ttgatgcagc ggcagaaaac ggtcgtcgag tagttttaac gggttttgat attgaaaata
    10681 ttgttcgtac ggccattcgt atgaaacgca ttcatattgc tgatgaaaat atgattatca
    10741 aacctaaaga tatgacacgt tacgaagata acgaattatt aatccttgaa actggacgta
    10801 tgggagagcc gataaatggt ctccaaaaaa tggcgatcgg acgtcatcgt tatgttcaaa
    10861 ttaaagatgg tgatttagta tttatcgtaa caacaccaag tattgcaaaa gaggcagttg
    10921 tagcgcgtgt tgaaaatctg atttataaag ctggtggctc agttaaattg ataacacaga
    10981 atttgcgcgt atcaggtcat gcaaatggtc gcgaattaca attattaatg aatctattga
    11041 aacctaaata ccttttccca attcaaggag agtataggga tttgtcagca catgcgggct
    11101 tagctcaaga ggtaggaatg tcagcagatg atatttatat tgtgaagcgt ggtgacatca
    11161 tggttcttga aaaagatggc ttcttccata gtggatctgt tcctgctgga gatgttatga
    11221 ttgatggaaa tgctattggt gacgttggta atattgtact tcgtgaccgc aaggtattat
    11281 ctgaagatgg aatttttatt gttgttataa ctgtcagcaa aaaagaaaag aaaattattt
    11341 ctaaagctcg tgttaatacg cgtggttttg tgtatgtgaa aaaaagcaga gatattttgc
    11401 gtgagagcgc agaattagtt aatacaacgg tagaagatta cttaagtaaa gatacatttg
    11461 actggggtga acttaaaggg aaagtgcgtg atgaggtcag caagttctta tttgaccaaa
    11521 cgaaacgtcg cccagcaatc cttcctgtgg ttatggaagt tcgttgatag tgttaagagg
    11581 ttaccaaatt ttggtaacct ttcttgctag aatggaggtt tttatggcat tttttaatat
    11641 tgaatatcac tctaaggtac taggaacgga acgtcaagtc aatgttattt acccagatgc
    11701 ttttgagatg tctgatgata aaattgatga ttgtgacatt cctgttttgt atcttctcca
    11761 tggaatgggg ggaaatgaga attcttggca aaaacgcaca aatatagagc gtcttttacg
    11821 tcatactaat cttatcgtcg tgatgccatc aacagactta gcatggtata ccaacacaaa
    11881 gtatgggttg gattattttg atgcaatagc tattgagtta ccaaaagttt tgaaacgttt
    11941 ctttcctaat atgtctgata agagagagaa aaatttcatt gccggattat ctatgggggg
    12001 atatggagca tataaaatag ctctactgac taatcgtttt tctcatgctg cctctttgtc
    12061 aggtgcactt tcttttgact ttgatttatt attcaataat ggcaacgata acactaacta
    12121 ttggtcggga atttttggtg atttgaataa cactgataac atagagcgtc attcactaag
    12181 gcgttacgtt gagtcttttg atatgaagac taaattttat gcatggtgtg gttatgaaga
    12241 ttttcttttt gaagctaatg aagtcgcaat tgatgaactt cgtcaattag gtttaactat
    12301 tgactattcc aatgatcatg gtaaacatga atggtattac tggaatcaac aattagaaaa
    12361 agtcttagaa tggctccctg ttgattatgt caaagaagag cgcctttctt agtattagct
    12421 aaatcgttga aatccaagta aagtcttgtt atactaaatc attaagtttt gggaaattag
    12481 acacagaagg tgagataaaa aatgagaaca ctatttagaa tgatatttgc tattccaaag
    12541 tttatcttta gattgatttg gaatatcatt tggggaatat tcaagacagt tcttgttatt
    12601 gcgattattt tatttggctt gtattactat gcgaatcaca gtcaatcaga atttgctaat
    12661 caacttagtg acattattca gacaggaaaa acatttttaa attttgctga cactaatcag
    12721 ctcaaaaatt cctttgctaa tctagctacg gataatgttc atcattcaga aaatactaga
    12781 tgggataaaa atcaggcaac tatctatata gcaactagag attctgagtt ggcctctgct
    12841 tacaagcaag caatctccaa ttggaatgca acaggtgctt ttgtgttaca aacaacagat
    12901 aatcctaatg ctgatattat tgctaaggat tactcagatg ctaaaacaca agctgcaggt
    12961 gtagctgaaa ccgagagaaa tgctttaact aatcgtattt caaaggtaac agttaaatta
    13021 aacacatttt accttaggaa taaacagttt ggttatgacc atactaggat tgttaatacg
    13081 gccgaacatg aattaggaca tgctttaggt ctaggacata atgatcaaca acattccgtt
    13141 atgcagtcaa aaggttcgca ttatggcatt caagaagttg atattcaaac gctaaaaaac
    13201 ttatatgcat aaaaagatga gctaataaac tcatcttttt tgtttgaaca cttattttag
    13261 gtgatataag tgcgttagca atagattatt aaaaataaag aatttgacta tcactactaa
    13321 ctaaaatgca taaagaacaa agaaaatgtt ttcaaaaatc acttaaaaaa tggtataatg
    13381 tgtcttgaga tagtgttttg aatagactta tcaaaaatat tagataacat taacttaaaa
    13441 ggggaaaata tggacgaact aaaaaaactt gctggagtga ccgctgcaaa atacgttaaa
    13501 aacggtatga ttgtgggttt agggacaggt tctacagcat atttttttgt tgaagaaatt
    13561 ggacgccgtg tcaaagaaga gggattgcaa gtcataggcg ttacaacgtc aaaccgtacg
    13621 actgagcagg caagaggatt aggtattccg ttgaaatcag cagatgatat tgatgtcatt
    13681 gatgtcactg tggatggagc agatgaagta gaccctgatt ttaatggtat caaggggggc
    13741 ggtggtgcgc tgctcatgga aaaaatagta gctactccaa ctaaagaata tatctgggtt
    13801 gtcgatgaga gcaaacttgt tgaaacgtta ggtgcattta aattgcctgt tgaggttgtt
    13861 agatatggtt ctgagcgtct ttttcgggtt tttaaatcaa aaggttattg tccttctttc
    13921 cgtgaaacag aaggtgaccg ctttatcact gatatgggga actacatcat cgacctagat
    13981 ttgaaaaaaa tagaggatcc aaagcagtta gcgaatgaac ttgaccatac agttggtgtt
    14041 gttgaacatg gtttatttaa tggtatggtt aataaagtta tagtagcaag caaaaatggt
    14101 cttgacatac ttgaaaaata attatttaaa taatcggttt taggagctaa ccgtaacctc
    14161 cttagaaaga agatataatg tcacaatttg atcgcattca cttagttgtc ttagattcag
    14221 tgggtattgg agcggcacca gatgccaatg attttgtcaa tgcaggagtt cctgatggtg
    14281 cttcagacac gcttggccac atttcaaaaa cagttggact tgcggtgcca aacatggcta
    14341 aaatcggact tggtaatatt cctcgtcccc aagcgcttaa gaccgtccct gcagaagaaa
    14401 atccaagtgg ctacgcgacg aagttacaag aagtatcact tggtaaggat acaatgacag
    14461 ggcattggga gatcatgggc ttaaatatta cagagccatt tgatactttc tggaatggtt
    14521 ttccagaaga tattatcaca aagattgagg atttttcagg acgtaaagtt attcgtgaag
    14581 cgaataaacc ttattcaggg acagctgtta ttgatgactt cggaccacgc caaatggaaa
    14641 ctggtgagct catcatctat acctcagctg atccagtcct acaaattgca gctcatgagg
    14701 atatcatccc tctagaagaa ctctaccgta tttgtgaata tgctcgtagt atcacaatgg
    14761 agcgtccagc tctccttggt cgtattatcg ctcgtccata tgtgggtga
//
LOCUS       DATRSG010000055        14026 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRSG010000055 DATRSG010000000
VERSION     DATRSG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14026
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.055"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            6..548
                     /locus_tag="VBD89_001567"
     CDS             6..548
                     /locus_tag="VBD89_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO7136085.1"
                     /translation="MYHMILCNSDIMKILAIIKSLPLNDCWLCAGTLRNFIWNKLSGI
                     NETLTSDIDVVFFDKNISYEETVVLEQQLKDNYPQYDWELKNEFYMNTHSPNTPKYTS
                     SKDAISKFPEKCTAVGERLDDRNQLELYLPYGEEEILNFIVSPTPYFEEDLLRYNVYL
                     KRVDKKKWNNIWPRLTIIKK"
     gene            768..1187
                     /locus_tag="VBD89_001568"
     CDS             768..1187
                     /locus_tag="VBD89_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO7136086.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1387..1866
                     /locus_tag="VBD89_001569"
     CDS             1387..1866
                     /locus_tag="VBD89_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7136087.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLINGERFAGKKKFIRNIQPFFNAFKAD"
     gene            1988..2680
                     /locus_tag="VBD89_001570"
     CDS             1988..2680
                     /locus_tag="VBD89_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7136088.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGWG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            2677..3429
                     /locus_tag="VBD89_001571"
     CDS             2677..3429
                     /locus_tag="VBD89_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO7136089.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMVKHHLTLEEYITLLKENNQ"
     gene            3426..4001
                     /locus_tag="VBD89_001572"
     CDS             3426..4001
                     /locus_tag="VBD89_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO7136090.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            4096..5178
                     /gene="hrcA"
                     /locus_tag="VBD89_001573"
     CDS             4096..5178
                     /gene="hrcA"
                     /locus_tag="VBD89_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO7136091.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKESEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            5181..5753
                     /gene="grpE"
                     /locus_tag="VBD89_001574"
     CDS             5181..5753
                     /gene="grpE"
                     /locus_tag="VBD89_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO7136092.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEEIVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            5934..7763
                     /gene="dnaK"
                     /locus_tag="VBD89_001575"
     CDS             5934..7763
                     /gene="dnaK"
                     /locus_tag="VBD89_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO7136093.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            8052..9167
                     /gene="dnaJ"
                     /locus_tag="VBD89_001576"
     CDS             8052..9167
                     /gene="dnaJ"
                     /locus_tag="VBD89_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO7136094.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFEDIFSSFFGGGGMR
                     NPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGTSPVTCQKC
                     HGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTHKVSVKIPA
                     GVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLNIGFVQAAL
                     GDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVNIVTPTKLN
                     DAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            9285..10532
                     /locus_tag="VBD89_001577"
     CDS             9285..10532
                     /locus_tag="VBD89_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO7136095.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETIPRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            10603..11379
                     /gene="truA"
                     /locus_tag="VBD89_001578"
     CDS             10603..11379
                     /gene="truA"
                     /locus_tag="VBD89_001578"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO7136096.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIYGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            11342..12100
                     /locus_tag="VBD89_001579"
     CDS             11342..12100
                     /locus_tag="VBD89_001579"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7136097.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVETQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            12110..12574
                     /locus_tag="VBD89_001580"
     CDS             12110..12574
                     /locus_tag="VBD89_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7136098.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            12571..13140
                     /locus_tag="VBD89_001581"
     CDS             12571..13140
                     /locus_tag="VBD89_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO7136099.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(13177..14019)
                     /locus_tag="VBD89_001582"
     CDS             complement(13177..14019)
                     /locus_tag="VBD89_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO7136100.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGAVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
BASE COUNT         4659 a         2119 c         2917 g         4331 t
ORIGIN      
        1 ctaaaattta tcatatgatt ttatgtaatt ctgatataat gaagatttta gctataataa
       61 aatctcttcc actgaatgat tgttggctgt gtgctggcac attgagaaac tttatatgga
      121 ataaactttc tggaataaat gagacattga catcagacat tgatgttgta ttttttgata
      181 aaaatatttc ttatgaagaa acggtggtat tagaacaaca attaaaggat aactatccac
      241 agtacgattg ggaacttaaa aatgaatttt acatgaacac tcattctcct aataccccaa
      301 agtatactag ctctaaagat gctatttcga aatttcctga aaagtgtact gcagtgggag
      361 aaaggttaga tgatagaaat caactcgaac tttacctccc ttatggtgaa gaggaaatat
      421 taaatttcat tgttagtcct acgccatatt ttgaagagga tctattacgt tataatgttt
      481 atttgaagcg agtggataag aagaaatgga ataatatttg gcctcgttta acaatcataa
      541 aaaaatgata acgttactgg agttggctta acagctttta aatttttaga ctgacaaata
      601 gtatagacct ttaataaggt cgtttatcga taatcatagt gtactgtgtt ataatcgtta
      661 tagcataaaa acacgttgca gttggtagtc tgcaagcgtt cagtgaacgt cagtaacctt
      721 ccctccaggt cgtccattgc aatttaatat ttaaaggagg gggctgtatg aaaatgacag
      781 tctattttga tggtaatttt tggttgggtt tgattgagta cgatgatgat ggtgattaca
      841 aagtttttcg gtattttttt ggaaaagaac ctaaagatga tgatgttttt aattttataa
      901 atcataaact taatgatttg ataaaaaaat acgagtttgt taaaacggat atttctctaa
      961 agcgtacgaa tgaacacaaa aaaagtccta aaagaatgca aagagaaatt aatagagaaa
     1021 aacgtaagcc agtagtatct acaaaagcgc aattggcgat gaaaactatt catatgagta
     1081 taaaaaacga acgtcaatta agtcagaaat gtaagaaaaa tgagttaaga aagcatcggt
     1141 accaattaaa acaagaaaaa cgttaccaaa agaaaaaagg gcattgattg tgtcatcata
     1201 taaaaagctt gagagtatca tctcaagctt tttatatgtt aactaatatg agttactaaa
     1261 tagtctgata atgctcaatt aatgtgtttt gggaaaaaat catttcctta aaggacacca
     1321 ttgtcatgtt gttatcacct tacttttttt atacttagta tatgtttaag aaaggaataa
     1381 gttgttttga gagattttga ggagttgttt gataaagtaa agccaatcgt aatgaagctg
     1441 agaagaaatt attttgttca actttgggaa tatgatgatt ggattcaaga aggacgtatt
     1501 gtacttttta ggttgcttga agagcatccg tatttattgg ataatgaaag caaactattc
     1561 atttacttta aaacaaaatt ttcaaattat ttaaacgatg tattaagaca ccaagattgt
     1621 caaaagcgac aatttaataa aatgccttat gaagaaatca gtgaagttag tcattatgtt
     1681 aagagtaaag gactagtttt agatgactat attgcttatc gtgatacttt aacaaaagtg
     1741 gaagaaactt tgtcagatat tgataaagaa aaatttgaaa aactgattaa tggtgaacgt
     1801 tttgcgggga agaaaaaatt catacgaaat attcaacctt ttttcaatgc ttttaaagcg
     1861 gattaatcga ttttatttct gcagtagata gctgcagaat ttttttatag ggaaagttcg
     1921 ctgatatatt cctgtgaaag cctttgtttt ttgttataat ataataaaat gcttgtgagg
     1981 tgtcagtatg agtaaagttc gtttatatat cgctcgtcat ggtaaaacaa tgtttaatac
     2041 aattggtcgt gctcaggggt ggagtgatac tcctttaact acttttggag aactcgggat
     2101 taaagagctt ggttggggac taaaggcttc aaatattagt tttaaagagg ctttttctag
     2161 tgatagtgga cgtactttac aaactatgga aattattctc agggaagtgc aacaagaaaa
     2221 tattccctat acgagagata aacgaatccg cgagtggtgc tttggtagtt tggatggagg
     2281 atatgatggc gaccttttta atggcgttct gcctcgagtt tcaaacggag atatgagcca
     2341 tttgacacac gaagaaattg ctaatttgat ttgtcaagtt gatacggctg gctgggcaga
     2401 accgtgggca atattgagta accgtattct gtcaggcttc actgctatag ctaaaaaaat
     2461 agaggatatt gggggaggta atgcgattgt tgtcagtcat gggatgacta ttgcgacttt
     2521 tttatggtta atcgaccata gtacccctcg tagtttaggc ttagataatg gtagtgtctc
     2581 agtcgttgat tttgaagatg gaacattttc tattcaatct attggggata tgtcctatcg
     2641 agaaaaagga agagaaattt tagaaaaaac attgcaatga aaaaaaataa aattatccga
     2701 ttcagtttag ttggtgttct acttgcgata ctatgcttta gtctttttgc tttattgaag
     2761 cctaacagtc aacaatcatc atctcaaaag ttgaggaatg aggatataaa aaagacatcc
     2821 tctcaaaaaa gaaataagaa attacgatta ccagctgtat catcaaaaga ttggaacttg
     2881 attttggtca atcgtgacca taaacatgaa gaattaagtc cagatgtggt gcctgttgaa
     2941 aatatttatt tggataaacg tattacgaag caagctactc agtttttaga ggctgctaga
     3001 gcaattgatt cacgagaaca tttaatttcg ggttatcgta gtgttgccta tcaggagaag
     3061 ttgttcaatt cttatgttac tcaagagatg actagtaacc ctaatttgac gaggggacaa
     3121 gcagaaaagt tggtaaaaac ttactctcag cctgcaggtg ctagtgaaca ccagactgga
     3181 ttagcgatgg atatgagtac tgtagattct ttgaatgaga gcgatcctag agtagtcagt
     3241 cagttgaaaa agatagctcc acaatatggt tttgtcttac ggtttccgga tggtaaaaca
     3301 gcagaaacag gggtaggtta tgaagattgg cattaccgct atgttggggt agagtctgca
     3361 aaatatatgg tcaaacatca tttaacatta gaagaataca taactttatt aaaggagaat
     3421 aaccaatgag gaaacgtttt tccttgctaa attttattgt tgttactttt attttctttt
     3481 tctttattct ttttccgctt ttaaaccata agggaaaagt agatgctaat tctaggcaga
     3541 gtgttaccta caccaaagaa gaatttatac aaaaaattgt gccagatgcg caagatctag
     3601 gaaagtcgta cggtattcgt ccttcattta ttattgcaca ggcggctttg gattctgatt
     3661 tcggagagaa aatattagct aataagtatc ataatctgtt tggtttgctt gcagaaccag
     3721 gaacgccctc aattacctta aatgatagta gtacaggaaa aaaacaggaa aagcaattta
     3781 ctcattataa atcttggaag tattcaatgt atgattacct tgctcatata aaatctggag
     3841 cgacaggcaa aaaagattca tatactataa tggtgtctgt taaaaatcca aaaactttag
     3901 tgcaaaaatt acaagatagt ggttttgata atgacaaaaa gtacgctaaa aaaatgacgg
     3961 aaatcattga tttgtatgat ttaacaagat atgataagtg atattagcac tctttgaata
     4021 agagtgctaa tttttgatgt tttttccttg actatctttt aaaaaggagt ataataaaat
     4081 catagttagc agtcgatgtg taagagtgct aatttcagaa agaccgagag gggtgatgtt
     4141 tttgtgatta cacaacgtca aaatgatatt ttgaatttaa tcgttgagtt atttacacaa
     4201 actcatgaac cagtagggtc taaagcttta cagagaacga ttgattctag tagcgccact
     4261 attcgtaatg atatggcaaa actagagaag ttggggttat tggaaaaagc acacacttct
     4321 agtggacgga tgccgagtcc agctggtttt aagtattttg tagagcactc ccttagacta
     4381 gatagtattg atgagcaaga tatttatcat gttataaaag cttttgactt tgaagcattc
     4441 aaattagaag acatgcttca aaaagctagt catattttat cggagatgac gggctataca
     4501 tccgttattt tagatgtgga gccagctcgt caacgtttga cagggtttga tgttgttcag
     4561 cttagcaacc atgatgctct agcagtcatg actttagatg aatctaagcc agtgacagtg
     4621 cagtttgcta ttccaagaaa ctttttgaca cgtgacttga tagcatttaa agctatcgta
     4681 gaggagcgct tgctggatgg tagtgtcatg gatattcatt ataagttgag gacagaaatt
     4741 cctcagatcg ttcagaagta ttttgtaact acggataatg ttttacaact atttgattat
     4801 gttttttcag agctgttttt ggaaacggta tttgtagctg gcaaggtaaa ttctttaaca
     4861 tattcagatc tatcaacgta tcagtttttg gataatgagc agcaagtagc aatttctttg
     4921 aggcaaggtc tgaaagagag tgagatggct tctgtccaag tggcagatag tcaagaagcc
     4981 gcacttgcag atgtgtcagt tttaactcac aaatttttga ttccttaccg tggatttgga
     5041 ctcctgagtt tgattggtcc tattgatatg gattatcgta ggtctgttag tttggtaaac
     5101 attatcggaa aggtattggc tgctaaatta ggtgattatt atcgatacct aaattctaac
     5161 cattatgaag ttcactgata atggcagtat tcaataagtt atttaaaagg aggcactcag
     5221 tgtcagaaga aattaaaaaa gacgatttac aggaagaagt ggaagccact gaaactgaag
     5281 agatagttga ggaagttatt gaagaaattc ctgaaaaatc agagttagaa ctcgcaaacg
     5341 agcgtgctga tgagtttgaa aataaatact tgcgtgctca tgcagaaatg cagaatatac
     5401 agcgtcgctc tagtgaagaa cgtcaacaac ttcaacgcta tcgttctcag gatctggcaa
     5461 aagcaatctt accatcgctt gacaatctag aacgtgcctt ggctgttgaa gggttgacag
     5521 atgatgttaa aaaaggttta gaaatgactc gtgatagcct tattcaagca ctaaaggaag
     5581 aaggcgtaga agaagtagaa gtcgactcat ttgaccacaa ctttcatatg gcagttcaga
     5641 ccctcccagc cgatgatgaa catccagccg atagcattgc agaagtcttt caaaagggat
     5701 ataaattgca cgagcgtctg ctaagaccag cgatggtagt tgtctataac taaggtaaat
     5761 gagttttcgt ttttgtccgt aatgacagta aactagatag caagttagaa gctattcagc
     5821 ttgctgatta aactatagtg attgcttaga attggaagta aaataattcg agtgcttact
     5881 aagataaatt gaaataaaaa gtaataaagt attataaaat aagaggtatt aacatgtcta
     5941 aaattattgg tattgactta ggtacaacaa actcagcagt agcagttctt gaagggactg
     6001 aatcaaaaat cattgctaac ccagaaggca atcgtacaac tccttcagta gtatcattca
     6061 aaaatggtga gattatcgtg ggtgatgctg caaaacgtca agcggtaaca aatccagata
     6121 ctgttatctc tatcaaatca aagatgggaa cttctgaaaa agtttctgca aatggtaaag
     6181 aatatactcc tcaagaaatt tcagcaatga ttcttcaata ccttaaaggt tatgctgaag
     6241 actatcttgg agaaaaagta gaaaaagcag ttattactgt tccagcttac ttcaacgatg
     6301 cacaacgtca ggcaactaaa gacgctggta aaattgcagg tcttgaagta gaacgtatcg
     6361 ttaacgaacc aacagcagcc gcacttgctt atggtatgga caagactgac aaggatgaaa
     6421 aaatcttagt ttttgacctt ggtggtggta catttgacgt atcaatcctt gaattaggtg
     6481 atggtgtctt cgacgttctt gcaacagcag gtgataacaa acttggtggt gacgactttg
     6541 accagaaaat tattgatttc ttggtagaag aattcaagaa agaaaatggt attgatcttt
     6601 ctcaagacaa aatggctctt caacgcttga aagatgctgc tgaaaaagct aaaaaagacc
     6661 tttcaggtgt aactcaaact caaatttcat taccgttcat cactgctggt tctgctggtc
     6721 ctcttcactt ggagatgagc ttatcacgtg ctaaatttga cgatctcact cgtgaccttg
     6781 ttgaacgtac gaaaactcca gttcgtcaag ctctttcaga tgcaggcttg tcattgtcag
     6841 aaattgatga agttatcctc gttggtggat caacacgtat cccagcagtt gttgaagctg
     6901 taaaagctga aactggtaaa gaaccaaata aatctgttaa ccctgatgaa gtggttgcca
     6961 tgggtgctgc tatccaaggt ggtgttatca ctggggatgt gaaagacgtt gtacttcttg
     7021 acgtaacacc attgtcactt ggtattgaaa caatgggtgg tgtcttcact aaattgatcg
     7081 accgcaacac aactatccca acatctaaat cacaagtctt ctcaacagca gcagacaacc
     7141 aaccagccgt tgatatccat gttcttcaag gtgaacgccc aatggcagca gataacaaaa
     7201 cactcggtcg cttccaattg actgatatcc cagctgcacc tcgtggaatc ccacaaattg
     7261 aagtaacatt tgatatcgat aaaaatggta ttgtatctgt taaagctaaa gatctcggta
     7321 ctcaaaaaga acaacacatt gttatccaat ctaattcagg attaactgat gaagaaattg
     7381 ataaaatgat gaaagatgct gaagcaaatg ctgaagcaga tgcaaaacgt aaagaagaag
     7441 ttgatcttaa aaatgaagtt gaccaagcca tctttgcaac agaaaaaact attaaagaaa
     7501 ctgaaggtaa aggctttgat acagaacgcg atgcagcgca atcagcactt gatgagttga
     7561 aaaaagctca agaatcaggt aaccttgacg acatgaaagc taaacttgaa gctcttaacg
     7621 aaaaagcaca agctcttgca gttaaacttt acgaacaagc agctgcagca caacaagcag
     7681 ctcaaggggc tgaaggtgca caatcagctg attcatcaag caagggtgat gatgttgtag
     7741 atggcgaatt cactgagaaa taattattaa tattgttcag attcatttga atataagcat
     7801 gaaaactata ctagcatagt aaagttcttc gtgatagggg ttgctcaata atctagataa
     7861 gtttcagatt acataagcta atttcgctat cactaaataa aaacatatta ataataaata
     7921 ggcggggcgc ctcgctccgt ctgttttatt aagtgtcata tatatgttaa ctatttagag
     7981 ctgtaactgg gcaagaataa ttgttaatct cttcaagtgt agtatatgaa caaaatataa
     8041 aggattagat aatgaacaat acagaatttt atgatcgtct tggcgtttca aaagatgctt
     8101 ctcaggacga aataaaaaaa gcttaccgta ggatgtctaa gaaataccat ccagatatta
     8161 ataaggaagc gggagcagag gaaaaatata aagaggttca agaagcttat gaaaccttga
     8221 gtgatacaca aaaacgtgct gcctatgatc aatatggcgc tgctggggca aatggcggtt
     8281 ttggtggctt cgatggtggt ggctttggtg gttttgaaga catcttctca agcttttttg
     8341 gtggaggagg gatgcgcaat cctaatgcac cacgtcaagg agatgaccta caatatcgtg
     8401 ttaatttaag ttttgaagaa gctatctttg gagctgaaaa agaagtatct tataatcggg
     8461 agtcatcatg tcatacttgt tcaggaagtg gagctaagcc aggtactagc cctgtgactt
     8521 gtcaaaagtg tcatggttcg ggtgtgatta atgttgatac acaaacacct ctaggaacaa
     8581 tgcgtcgcca agtgacttgt gatgtttgtc agggtagtgg tcaagaaatc aaagagaaat
     8641 gtccaacatg tcatggaact ggtcatgaga agaagacgca taaagtatca gttaaaatac
     8701 ctgcaggagt tgaaactggg caacaaattc gtttaacagg acaaggagaa gctggcttca
     8761 atggtggtcc ttatggtgat ctctttgtta ttattaacgt tctcccaagc caacagtttg
     8821 agcgtaatgg atcaacaatt tattatactt tgaatattgg ctttgtccaa gctgctttgg
     8881 gtgatacaat tgatatacct actgtccatg gagcggtaga aatgtcaatt cctgctggta
     8941 ctcagacagg aaaaacgttc cgtcttcgcg gaaaaggtgc ccctaaactt cgagggggtg
     9001 gacaaggtga tcagcatgtt actgttaaca tcgtaactcc tactaaattg aatgatgctc
     9061 aaaaagaggc attgcacgct tttgcagagg caagtggaga taagatggta catccgaaga
     9121 agaaaggttt ctttgataaa gtcaaagatg ctttagatgt tgattaaaaa ataaaagtct
     9181 agaatcgaaa gtattctaga cttttttgta gttattgcta gagggattgc ttgaacctaa
     9241 attttacagt acaatgaatc taatattgta gggggacaat ttttatggta accaaagttg
     9301 aagagattcg ctcatatttg atagcttcta tacaaaatgg taagttggct ccaggagatc
     9361 gcctaccatc tataagacag ttagctaatc aattttcctg taacaaagat acagtgcaac
     9421 gagttttgat ggaattgcgt tttgataatt atatctatgc aaagcctagg tcaggctatt
     9481 acgtctttga ttctcatcaa gaggaagttg aagaaggggt tagtttacca aactctgaga
     9541 ttgcaaatat agcttatgat gattttagat tgtgtttgaa tgagaccctt attggtaggg
     9601 aagattacct tttcaattat tactatcgtc aagaaggtct tcttgattta agtaaagcag
     9661 tggctaaatt aatggaagaa acaggggtct atgttcccct tgatgatatt gttattacgg
     9721 ctggtactca acaggcatta tttattttga cacaggttac ctttccaaat cgaaaatctc
     9781 gagttttaat agaagaaccg acttatcctc gtatgattga actaatcaaa acacaaaatt
     9841 taccctatga aactattcct cgaggtactc atggaattga ttttcagcgc ttagaggaga
     9901 ttttccagac acaatcaatt aagttttttt atgttatacc tcgcatgcat aatcctttgg
     9961 gaacatccta taatccggta gagatgaaaa gattaataga gatggcagag aagtatgatg
    10021 tttatattgt ggaagatgac tatatgtctg attttgcaag tcagtcacca ttacattatt
    10081 atgatactca cgggcgtgtt atttatctaa aatctttttc aaaggctatt ttccctgctt
    10141 taagattagc tgcgatttgt ttaccacaag ctttaaaatc aacatttatg gcttacaaga
    10201 agttgatgga ttatgatact aatctgattt tacaaaaagc attagcgctt tatattgaaa
    10261 atggccttta tgctaagaat agtcaatatt tgaaatatcg ttatcagaaa gaccttgcaa
    10321 attcaaaatc tattttagct gatcacccta atctaccccc atatagttta catcacgata
    10381 gtgtattatt tgattgttcg aaactcgata acttcaaaat attacggcaa tacggcgata
    10441 ctttggaaaa ttatttttgt caaaaatcgc atcaatctct cttacaagta aaaaatgatt
    10501 cctgcttaaa gcagttcttg ggatcgttgt agtataattg tagggggaca gtttaaaata
    10561 gatattatag ttttcataaa tgaaaggtgg taaaataaac ccatgacaag gtataaggca
    10621 cagatttctt atgatggttc agctttttca ggcttccaaa gacaaccaaa ttgtaggaca
    10681 gttcaagaag aaattgagag aactcttaag cgcctaaata gcgggaacga tgtgataatc
    10741 tatggtgctg gtcgcacaga tgctggtgtt catgcttatg gacaagttat tcattttgat
    10801 ttaccacagg cgcgtgatgt agaaaaatta cgctttggtt tagatacaca gtgtccagac
    10861 gatattgata ttgttaaagt agagcaagtg tctgatgatt ttcattgtcg atatgataaa
    10921 cacattaaaa cttatgagtt tttagtagat atcggtaggc cgaaaaatcc tatgatgaga
    10981 aactatgcaa cacattaccc ttatcctgtc attattgagc ttatgcaaga agctattaaa
    11041 gatttagtag gcacccatga ctttacaggc tttactgctt caggtacgag tgtagaaaat
    11101 aaagtacgta ctatttttga tgcgaaaatt cagtttgaag cgtctaaaaa tcttttaatt
    11161 tttaccttta caggaaatgg ttttttgtat aaacaggtgc gtaatatggt tggtaccctg
    11221 ctaaaaattg gtaatggacg catgcctatt agccaaatca aaacaatttt acaggctaaa
    11281 aatcgggatt tagcaggtcc aactgcggca ggaaatggtt tatatttgaa ggagattatt
    11341 tatgaagacg aggaatgttt tagcaatttc aggaaatgat attttctctg gcggcggctt
    11401 gcatgcagat ttagcaactt acgttgttaa taaattacat ggttttgtgg cagtaacatg
    11461 cttaacagca atgtcagata aagggtttga agtcattcct atagaggcat ctatactaaa
    11521 gcagcaacta gaaagtttaa aagatgtaga gtttgggtct attaaactag gattgcttcc
    11581 caatgtagaa acagcacaag ttgttttgga atttgtaaaa tcaaaacaag aatgtccagt
    11641 agtcttagac ccagtcttag tttgtaaaga aaatcatgat ttagaagtta gccaattacg
    11701 tgagcagcta attgcgtttt tcccatatgc tgatgtgatt actcctaatt tagtagagac
    11761 tcaattattg acgggtcttt caattgaaaa cttagatcaa atgaaaattg ctgctgagaa
    11821 actttacgat atgggggcta aacatgttgt tatcaaaggg ggcaaccgtt taaatgctga
    11881 agaggctaca gacctttact atgatggtga acgttttgaa acctatgttt ttcctgtagt
    11941 agatgccaat aatacaggtg ctggatgtac atttgcgtca agtattgcca gccaattagc
    12001 tatgggtaaa aatgtagaag atgcagtcaa aatgtctaaa ggtttcgtct atcaagctat
    12061 taaagcatcg gataagtacg gagttgtcca acatttttag gagattttaa tgaggaaaga
    12121 gaaaactagt caattggttc aattggcaat tgtaacagct atatctattg tattggggat
    12181 gtttataagt attccgacac caacaggttt tttgacatta ctagatgctg gaatattctt
    12241 tgctgccttt tactttggca aaaaagaagg ggctgtggta ggtgccttag ctggtttttt
    12301 aattgattta ttgaaaggtt atcccaattg gatgttcttt agtttattga ttcatggggc
    12361 gcaagggtat ttagcgggtt taccaggtag aagacgtctt ttaggtctta ttagtgcaac
    12421 tcttgttatg gtattaggtt atgctatagc tagtggattg atgtatggtt ggggagctgt
    12481 tttaccagat attcctggca atattatgca aaatatggtt ggtatggtcg ttggttttgc
    12541 tcttaataag tctctagaaa gagtgaagaa atgatactag attataataa gttaaagcag
    12601 gaaactaaag ctattgtagt tgatattatt gagcgtagtg ctcttaaaaa aggtcaaatt
    12661 tttgtactag gtctatcatc tagtgaggtt tctggtggtt taattggaaa aaattctagt
    12721 tctgaaattg gtgaaattat cgttgaagtt atcctcaaag agttacattc acgcggcatc
    12781 tatttagcag ttcaaggttg tgaacatgtt aatagggcgt tggttgttga agcagaattg
    12841 gctgaacgtc aacagttgga agtcgttaat gttgttccta atctacatgc tggcggtagt
    12901 ggtcaggttg cagcttttaa gttgatgaca tcaccagttg aggttgaaga aattgtggca
    12961 catgctggaa ttgatattgg agatacttct attggaatgc atatcaagag agtacaagtt
    13021 cccttgattc caattagtcg tgagttggga ggggcacacg ttacagcact agctagtcgt
    13081 cctaaattaa ttggcggtgc tagagcagga tatacgagcg atccaatccg taaattttaa
    13141 aataaacctc tgctatgcag aggtttattg tttatctcaa cgagagtggt tattggagaa
    13201 attgtatgtt ggtaagtcaa taccttcttc aagaatagct ttttgataaa gtttgtaaaa
    13261 ttcagcatag atgtcaaact gagcaccgtt ttgcgtgaag atattaatac gataaacaaa
    13321 ttgaccatta gcattggttg taggaccaaa aacagtaggc tctccaacga tagcaggatg
    13381 ttttgaaaca tactcttcat tgatttttgt tacaatatca gaaatctgat caaggtttgt
    13441 atgaacaaat aaaggaatat caatttgagc acgcatattg ctacgagact tattgctgac
    13501 aattgtaata ttacgattag gaatgaagtg taaggttcca tcgaaatcat gaatttgtgt
    13561 ggtgcgtatc ccaacattag taacagtgcc tgatacagca ccaacattaa taatatcacc
    13621 aacatcaaat tggttttcta gcagaataaa aaagccattg acaacatctg ataagaagcc
    13681 ttgagcacca agtccaatag caaccccagc aataccagcc ccagctaata aactagaaat
    13741 tggaacgccc aatatactta aaatccaata gaatagaaag aaataaaggg tatagtttaa
    13801 aacattgtga gaaagtttag ctaatgtttt ttgacgagct actttttgtc tagaaaaggc
    13861 gagtgagcga ttaacagtct tttcgaaaag ataattgatg acttgcctaa cgataacgaa
    13921 tgcaatgatt aataataaga ttgatattag tttggtgaag aaagtaaata aaacttcctc
    13981 tacattaaga tggtcaataa atttttctaa agtaatcata gtaata
//
LOCUS       DATRSG010000056        13608 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRSG010000056 DATRSG010000000
VERSION     DATRSG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13608
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.056"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(179..1516)
                     /gene="brnQ"
                     /locus_tag="VBD89_001583"
     CDS             complement(179..1516)
                     /gene="brnQ"
                     /locus_tag="VBD89_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7136101.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDLEKFEG"
     gene            complement(1712..3709)
                     /gene="metG"
                     /locus_tag="VBD89_001584"
     CDS             complement(1712..3709)
                     /gene="metG"
                     /locus_tag="VBD89_001584"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO7136102.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLDEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            3852..4727
                     /gene="tehB"
                     /locus_tag="VBD89_001585"
     CDS             3852..4727
                     /gene="tehB"
                     /locus_tag="VBD89_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO7136103.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            complement(4885..5580)
                     /locus_tag="VBD89_001586"
     CDS             complement(4885..5580)
                     /locus_tag="VBD89_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7136104.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            5916..7133
                     /locus_tag="VBD89_001587"
     CDS             5916..7133
                     /locus_tag="VBD89_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7136105.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYSYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            7117..7959
                     /locus_tag="VBD89_001588"
     CDS             7117..7959
                     /locus_tag="VBD89_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7136106.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            8036..8863
                     /locus_tag="VBD89_001589"
     CDS             8036..8863
                     /locus_tag="VBD89_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO7136107.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(8903..9259)
                     /locus_tag="VBD89_001590"
     CDS             complement(8903..9259)
                     /locus_tag="VBD89_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO7136108.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(9261..9737)
                     /locus_tag="VBD89_001591"
     CDS             complement(9261..9737)
                     /locus_tag="VBD89_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO7136109.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(9792..10942)
                     /locus_tag="VBD89_001592"
                     /pseudo
     CDS             complement(9792..10942)
                     /locus_tag="VBD89_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(11015..11563)
                     /locus_tag="VBD89_001593"
     CDS             complement(11015..11563)
                     /locus_tag="VBD89_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO7136110.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(11631..12722)
                     /gene="serC"
                     /locus_tag="VBD89_001594"
     CDS             complement(11631..12722)
                     /gene="serC"
                     /locus_tag="VBD89_001594"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO7136111.1"
                     /translation="MIIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNITRGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKVQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENT"
     gene            12855..13490
                     /locus_tag="VBD89_001595"
     CDS             12855..13490
                     /locus_tag="VBD89_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO7136112.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVKQAHGTRITQ"
BASE COUNT         4486 a         2590 c         2123 g         4409 t
ORIGIN      
        1 ctagaaaaaa atgtatattt tcaatccaca gctgttataa aataattttt aaaaagtgtt
       61 tatatcttag tttaatcaca ccttactgca atctatatat catttaaaaa aatctgatat
      121 aaaaaaggct aaaactaagt tttaaccttt ttgacatatc tataaaattg aaatagtgct
      181 atccttcaaa tttctctaaa tcaaatgctt ggcctttttg tttatctgac agcaccaaag
      241 aaaatactat accaatcaac attggaacta gccaccccat tgaaatagta tgaaagggga
      301 ggaatccaac taactgagtt aatgtttttt cttgccattg tcccgctaga acctcaacaa
      361 acgacactaa agtgacaaga ccaattgtca aagacattcc cttttttgaa agcggcaacc
      421 acttattaat caaaataatt aagacaataa caataacaat cggatacaat aaagttagca
      481 caggaactga aaaagtaatt acagcattta aaccaagatt agcaattaaa aagccaatca
      541 aagtaaaaac agtagcaaat aatttataat tcccaaaacg aaagttctta tcaaaaaatt
      601 cagatactga cacaataagc ccaactgttg ttgtaaagca tgtcaatgtt accataatac
      661 tcaagaaata acgaccaaaa tttccaaata atttataaga tgcttgtgat aatacataag
      721 ctcctttatt aacatttggg tcagctaaaa tatcagctgg gacaggaaac ttattcccta
      781 aaaaacctaa gccaatatat aaaatactaa atgctaaact agtaacaatt ccaacaatcc
      841 aaatggttga taaatattct tttttagttt taaaaccaaa ctttttaagc gtttctgttg
      901 ctactagaca aaaagcaact gctgcaagtg catcaagtgt attatagcct gctaagacac
      961 cacttccaaa cgcttttcct gcatagtcag cactcgcctt agcactctcc aagtttccat
     1021 aagcaatagc tccaacaaca accaaagaga gaattaaaaa ggcaaaaaca ggcgttaaga
     1081 tttttccaac actatctaat ataccatttg gtctaatagc gagataataa gctgcagcaa
     1141 aaaaacaggc tgtaaaacac aaaagagcaa tcggactatg ccctacaatt ggagcaaccc
     1201 caatttcaaa agaaaccgtt gctgttctag gaatagcaaa aagtgggcca atagttaggt
     1261 agagtataac taaaaatgtt aaagaaaacc acggactaaa tttttccgac atctctgtct
     1321 tataaccacc attagttagt gtgcctagta ggagggtaat aatagctaat ccaactcctg
     1381 acaaacaaaa tcctaaaatc gctggccaga aatcttgtcc agatgctaca cctaatgctg
     1441 gtggaaaaat gaggttccct gcaccaaaga agataccaaa taagagtaat cccgttaaaa
     1501 agcctttttt taccataagc aaccattagt gtaaataatc tatttacacc ctttccataa
     1561 attctcctta tcaaattcta tctaaatgct tagaatcgat gtcttttatt atatcaaaaa
     1621 taaaaataaa ttctaggtca ttttcataat tttccaacta ttcagtcact tttttcttga
     1681 aaacaaaaaa agagcataat gctcttttcg attaaccaat aattgatcca tttgccactg
     1741 ctgaatcgac cgtcaacact gtcaatttac catcatgttc tgctgagaga atcatgcctt
     1801 gactgacata ttttttcatc attttacgtg gtttgaggtt agcaacaatt tggagttttt
     1861 tccctactaa ctcttgttca tttgggtaaa attttgcaat ccctgataag atttgacgat
     1921 ggccctcatc accagcatct agacggaaac gaagaagttt atcagaacct tccactttct
     1981 caacttcaat tacttctgca acgcggattt caacagcgtc aaaatcctca aacttgattt
     2041 gacctttgct tgatgtcaat tcaacttctt caggaaccca ctccttttcc actggtgctt
     2101 ttccagcatt catttgttct ttgatataag taatttcgtc ttccatatcc aaccttggga
     2161 aaattggtga accttttgca acaactcgaa caccttctgg tagatcagca aaagtcaatt
     2221 tttcaagatc gaatgttgcc cctaaaccaa gttgttccat aatggcatcg gaagtttcca
     2281 tcatgaacgg ttgaatgagg tgagcaacaa cacgtagact agcaactaaa tgactcatta
     2341 cagctgctaa cttatcacga tctgtttcgt cttttgcaag tacccacggt gctgtttcat
     2401 caatatattt gttagttcgt gaaataagat tccatacagc ttcaagggca cgtgggaaat
     2461 ccaccgcttc catttgttta tgatagtttt caatactatc tgtcgcaaca gaagctaaat
     2521 cggcatcaaa gtcagttgca acagcaaacc ttggtacttc tccatcgaaa tacttgttaa
     2581 ccatagcaat agtgcggttg agaaggtttc ctaaatcatt agcaagttca tagttaatac
     2641 gtccaacata atcttctggc gtaaaagttc cgtctgaacc aacaggcaat gaacgcatga
     2701 gataataacg gagtggatca agaccaaaac gctctactaa catttctgga taaacgacat
     2761 tcccttttga tttagacatt ttaccatctt gcatcacaaa ccaaccatga gcaactagac
     2821 gttttggtaa tggcaaatcc agcgccatca acatgatagg ccaataaata gagtggaaac
     2881 gaagaatatc tttaccaatc atatgaatgt ctgctggcca aaatttatga tattgactga
     2941 gatcgtcaga ccaaccataa ccaagagcag aaatatagtt catcaaagca tcaatccaga
     3001 cataaataac atgttttgga tttgatggta cctgaactcc ccatgtgtaa gttgtacgag
     3061 acactgctaa atcttctaaa cctggttcta taaagttttt aagcatttca ttcatacgac
     3121 catctggctg aataaattct ggatgctcag cgtagtacgc ttttaaacga tctgcatatt
     3181 tgctcatgcg gaagaaataa gattcttcag aaactttttc aacttcatga ccagatggcg
     3241 caacaccacc aatcatatta ccgttttcgt cacgataaac ttctgctaat tggctttcag
     3301 taaaaaattc ttcatctgaa acagaatacc atccggtata ctcatcaaga tagatgtccc
     3361 cttgtgccaa caattgttca aaaattttag caaccgcttc ttcatggtaa gtatctgttg
     3421 tacggatgaa tttgtcatag gaaatatcta aaagttccca taatgttttg acactttcag
     3481 ccatgccatc aacatattcc tgtggtgtaa tgcctgcttc ttcagccttt tgttgaattt
     3541 tctgaccatg ctcatcaaga ccagtcaaat actgaacatc aaagcccatc atgcgtttgt
     3601 aacgtgccaa aacatcacat gctatcgttg tataggcaga accgatgtgc aatttacctg
     3661 atgggtaata aattggggta gtaatgtaaa atgatttttt ttctgtcatg atttctccta
     3721 taagccaccc taatgagagg tgttttttat catgatacat tataacataa aatctttttc
     3781 aactggatat atctctgaat aatatatcta cttttgctat aataaaggta ttaaaaatag
     3841 agagttaata tatggttaat acaaaaacac ttctccccta caaaactatg ccggtttgga
     3901 ctgcacagtc tattccaaag gcatttttag aaaagcataa tactaaggaa ggcacctggg
     3961 caaaactaac cattctaagt ggttctttag tattttacca gttatctcct gatggagagg
     4021 aaatctcgcg gcatattttt gatgctagta gtgatattcc ttttgttgat ccacaagtct
     4081 ggcataaagt ttcgccgaat agtccagact taagttgcta tctaactttt tactgccaaa
     4141 aagaagatta cttccataaa aaatatggtc tcacgcgcac acattctgag gttatcgcca
     4201 gtgcacctct cttatctgag aagagtaata tattagacct tgggtgtggt caagggcgaa
     4261 actcacttta tttatcgctg ctgggacatc aagtgacttc tgtcgattca aacggacaga
     4321 gccttgtagc tttagaaaat atggcattag aagaagagct tccttacaat ataaaaaggt
     4381 atgatattaa tactgctgct attgaagggc actatgattt tattttatca actgtggtat
     4441 ttatgttttt aaatcccgac tgcatttctg atattatttt acaaatgcaa tcacatactc
     4501 agattggagg atacaatctt atcgtttctg ctatggatac cgctgaaaat ccttgcccgc
     4561 ttccattccc ttttaccttc aaagaaggac aattgaaatc ttattacaat gactgggaaa
     4621 tcatcaaata taatgaaaat ttaggtgaat tgcacagggt ggatgaaaac ggtaatcgct
     4681 taaaattaca gtttgcgact ttattagctc gaaaattaag atcataaaag tgagtagaaa
     4741 tctactcact tttatctttg gttaggtaat aagtatattc taaacccata cataattatc
     4801 ctataaaata tatagagatg atatttcaaa tcattattgt aacaatcagc tttagtatgg
     4861 ctcttcaaca taaaacgcca tacattagga gtgcctttct acttgtaggt tataaaattc
     4921 atggcctaac agcaagttga catataaaag aggaattaat attaatgata agctccattc
     4981 gcccagttta cttgaaaaat aagtcgagca aacaacacca aagataaatc ctccaataat
     5041 aataatcgta tttctagcaa ttaagaaaat atcagaattt ttttcgtaaa gtccctttgt
     5101 taataaataa gcagcattct tgatattacc agtcatcatc atattagcat aaggtgctcc
     5161 tctgagcgat ttaaaggtat ctacttgtat tgaagcaaag aaggctagtc ctgccacagt
     5221 aaacgacgat ggtaagaaag gagtcaatat aattgttact atagcaacct gtgtcaacgc
     5281 aaaactagat aataggtacc agtgcagttg atgtttattc gcccaacgat tcatgaaata
     5341 agtaaacgat tgacctaaca tgaaaaccat tataggtagc aaaaaagcaa gcgcttgact
     5401 ataatgctta ttactaagat gaatagcaaa agataacaaa ttccctgttt gtacacctgc
     5461 aaaacgtttt ccttgtgtta cataagtaaa agcattgata taacctgaaa taaaacagag
     5521 tgcaaccgca cacctgagcc cttcatatat ttgatattga cggtgagact ttttagacat
     5581 attttcctct aactttatgg caaacaaaaa taagactggg caatcttgct cagcactttt
     5641 tttctaatag attggtttaa aatagtatta atattagcac tagctagcct aacagttcta
     5701 aataaattat taagcaatga taaatgtagt tttagtatat catagcctca gcattgaagc
     5761 aatctcactt tatgaaagga taaagaaaat ctcataagtc atttaccctt aatttgaagt
     5821 ttaattagtt acttttaatt ttactaccaa aaaacttgca ccattcttaa catttgttac
     5881 aataaataca acctattagg aggtcagtac agattatgca cagcactatt tataaatgtc
     5941 atcattgccg ttatctaatt tataaaagtt ttaaagatat ctttcctttt gctctcattt
     6001 tttcttggat tgaaatcctt caaaaactcg ttatatcaga tgattctttt ttagctcaaa
     6061 caacaaagtc cagtatttat aaaaacgagt ggttatatac ggtttccaat actctttatg
     6121 atttatctca tttactaatc tactttatgg ctagttattt tgtggctagt ttagtatgga
     6181 gaggcttatc taaccaaact aaagaaagtt tttctgcaag tttacttggc tttgccactg
     6241 tttggttgct tctaacttca gttgttccag gtacagatta tcactatcct agccaaccca
     6301 tttggcttat gctggctttg atttcgtatc tttttatctt tttaactcga ctgcttcaaa
     6361 atcgacatca tattttccag tatagtatgt tagctatact actattaatc tcttctattt
     6421 tgtcacatat cttatatagt tatcctaata ttgatttaga ataccttata caaaatcact
     6481 tttctcaatg gctgggtgat gggccacatc aattaattcc tattatgctt tggtctatct
     6541 tagctatttt tctaacctta tcaggtcttt ttgtaccaga tattttattt cgtccttact
     6601 cctatttttc agtagttagc gaaaatttaa atgctgcctt gagccaacat acagataaaa
     6661 tcccttatct gtataccttt tatactgtta aaaatagctt tgctatgttt ggcggtattg
     6721 gaatcctatt atccttattt ttagcagttt tatatgaaag tagaaaacta caatcgaaga
     6781 attactataa attgacatta ctgaccttaa ctcctcttat ttttgatcaa aatttaccat
     6841 ttttagtagg tctacctgtc attttacaac caatattatt cattccgatg gtattaacaa
     6901 ctatctttgc agaggctttt ggagctctta tgctctacct aaaatttgtc gaccctgcag
     6961 tttatactgt cccaagtggt acacctagtc tactatttgg ctttcttgct tcaaatgggg
     7021 attggcgtta ccttccagtc actgcaatta ttctagttgt tggcttcttt atttatcgtc
     7081 cgtttgtcaa aatcgctttc gctaaggagg agcaatatga aaaaattact tagatggctt
     7141 cctcctgtac ttttcattat tatccttata ggaatgacta tcttaggtaa gtcctatatc
     7201 aataaagtaa cagctcacaa aataaaactc tataactctc gaatgactcc tactatttta
     7261 attccaggat ccagtgctac tcaagaacga tttaacagca tgttagcaca gctcaaccaa
     7321 atgggagaaa aacatagcgt tttaaagtta actgtcaaaa aagacaatag cattatctac
     7381 aatggacaaa ttagcggcaa tgaccacaaa ccctacattg tcattggatt tgaaaataat
     7441 gaagatggtt atagtaacat caaaaaacaa acaaaatggc tacagattgc tatgaatgat
     7501 cttcagaaga aatataaatt taaacgtttt aacgctatcg gtcattcaaa tggtggctta
     7561 tcatggacta ttttcctaga agattattac gactctgatg aatttgatat gaaatcattg
     7621 ttaacaatgg gaacaccttt taactttgaa gaaagtaaca cctcaaatca tactcaaatg
     7681 cttaaagatt taatcagtaa taaaggaaat attccatcaa gtctcatggt atacaatttg
     7741 gcaggaacta attcatatga tggtgataaa attgttccat ttgctagtgt ggagactggt
     7801 aaatatattt tccaagaaac cgctaaacac tatacccaac taacagtaac tggtaataat
     7861 gctacacatt ctgacttgcc tgataatcct gaagttatcc aatatgtcgc agaaaaaatt
     7921 cttaaaaatg agaaaggtaa attaccaaaa cctcactaaa gctgtagaat agcaacgcct
     7981 ctggtatttt gatataatat attgcgaatt aaactaaata aaaaaggata ttattatgaa
     8041 actcatttca tggaatattg attccctcaa tgcggcatta acaagtgaat caacacgtgc
     8101 tttaatgtca cgccaagtta ttgatacttt agttgctgaa gatgccgaca tcattgcgat
     8161 tcaagaaact aaactgtctg ccaaaggacc tactaaaaaa catttggaag tacttgaaac
     8221 ttacttccca gaatatgatt tggtttggcg atcatcagtc gaacctgctc gtaagggcta
     8281 tgctggtaca atgttcttat atcgtaaagg gttaaatcct attgttagct tccctgaaat
     8341 cgatgctcct actacaatgg ataatgaagg tcgcattatt actctcgaat tggaaaactg
     8401 ctatatcaca caggtctata ctccaaatgc aggtgatggc ctaaagcgct tagctgaccg
     8461 tcaaatatgg gatattaaat atgctgagta tttagcaaca ttggatagtc aaaaacctgt
     8521 tctagcaaca ggtgactata acgtagccca caaggaaata gacctagcta atcctagcag
     8581 taatcgtcgt tctgctggat tcacagctga agagcgccaa ggctttacaa atctactagc
     8641 caaaggtttt acagatactt ttcgttacct tcatggcgat gttccaaatg tttatagctg
     8701 gtgggcacaa cgaagtcgta catccaaaat caacaataca gggtggagaa ttgattattg
     8761 gctaacaagt aaccgtgtcg cagataaaat taccaagtca gaaatgattc attctggtga
     8821 tcgtcaagat catacaccaa ttattttgga gattgaacta taaaccaaaa aaagatgatt
     8881 gagttataac tcaatcatct ttttatacta agttgaggtc tttatactta gtacggtagc
     8941 caatctgaag aattttatta tctttaacta gcaatggacg cttaataagc attccatccg
     9001 atgccaataa gtccaaagct tggtcaagag ttaaatcatc aaatttatct tttaagccta
     9061 attctctgta agaatttcct gaagtattga aaaacttttt aaggtcataa ggcgaattct
     9121 ctaacaattc ttttaataac gaagctttgg gaggattgct ttttatatca atcgcttcaa
     9181 aagtcaagcc gagttcagtt aactcttttt ttgctgaacg acaagtggta catttggggt
     9241 attcataaaa cgtatacata ttatttttcc ttttcgtatt ctaataacca agattttctt
     9301 tctaagccac cagcataacc agttaattga ccatatcttc ctaaaacacg gtgacaaggt
     9361 ataattagag aaataggatt tttacctatt gctcctccaa cagcctgtgc agattgacaa
     9421 tttagctctt tcgctaattc tccataagtt ttggtatctc caaaagggat tttctgtaat
     9481 tcctgccata ctctcgtctg aaatgctgtc cctctcaatg ataatggaat actaggtaaa
     9541 ttttgctgtg aaaaataagc atctaaccac ttcttaccaa gtaaagtata ggaatttgac
     9601 atattaacaa tttcctcttg gttgactgct agcatatcat atttttgccc aacaaaataa
     9661 agccctgata aacctagatt gtctgctaga aggcgaattt ctccaagagg ggactgatag
     9721 aattcttgat ataacatatg ataagcataa cttaaaaagc catgaaaagc aattcatgac
     9781 tttttagttc tttattgttg ttttgcaatt aaacgtacac ggacgatatt ctcatcacct
     9841 tcaaattctt caattaaagt agaaatcttg ttattatccg tttcatctaa atctattagt
     9901 gtgtaggcgt agtcaccctt tgaacggtta ataatgttat caatatttat acctaacact
     9961 gatacagcag ttgaaatttt agccataatg ttaggaacat tcttgttaat taaagtaata
    10021 cgaaatggtg cggtttgaat ttgatgtaca ttggggaaat taactgaatt agtgatttct
    10081 cctgtctcca taaaacaacg aatggtttga cttgccataa tagcacaatt tagctctgcc
    10141 tcatctgttg atccgcctac atgaggaaag actgtaattc ctttttggtt taataattct
    10201 ttgtctccaa aatcagtaat atagcgcttg acaacaccag tttctatcgc ttcaaatagc
    10261 tcttggttat taactaattc tgcacgagca aagttgataa tcgtagttcc ttttttcatg
    10321 attgaaaaag cttccgcatc aaaagtatgc ttagtttcat ttgttaaggg aacatgaatt
    10381 gtgatatagt cacaagtttc aaaaatatcc ttaatctctt taaccctttg aacatggctt
    10441 gaaatattcc aagctgtttc aattgaaata tagggatcat aaccaagaac tgtcattcct
    10501 aagcgtctag cacattcgca attctagctc caatggcacc aaggccgata actcccaatt
    10561 tttttcctgc aatttcatta ccagcaaaag ctttctttcc tgcttcaatt tgtttgggaa
    10621 tatctgttcc agttagagta ttaacccatc ggttagctcc taaataatca cgagctgaaa
    10681 gtaataaggc agcaatgacc gcttctttta cagcattagc atttgcacct ggggtattaa
    10741 acacgactat tccctgtgca cttgcctctt caataggaat attatttgtt cctgcacccg
    10801 ccctagcaat agctttgaag ttacttggga aatcctgatt atgcaaattt tgactacgaa
    10861 taatataggc atcaggattt tctgacatat ccccatcaat ctggaaacga ttccctaact
    10921 cttgtaaacc aatttgatta atattattaa atgttttgac actaaggtta tctctttagt
    10981 catcattgac caaaataacc cctttctttt tttcttacat gggatttctt tcatcaatct
    11041 cacgatagat gctcgtttct cccttttctt ttatcttctg ataagccaat cgtaccccat
    11101 ctagaggtac tttaccacaa tattgatagc ctaatttatt tagaatatgc tgcatagtga
    11161 catttttttc atgagtatcg caacgaaaat caggtccttt atgaccttca attaaccctt
    11221 gaaggaatgt ctgtgctagt cctctaccac gaaattgatt tgagatcgca atacgatgaa
    11281 atgtaagata gcgatggtta tcatggagcc attttccctc ataaatagca tcataaacct
    11341 cttcatgacc atcaatgaca gccgcataag tcgctagcat cccatcttca ataccaaccc
    11401 aagcataacc gttcaagata tcatcaataa tatcgttacg attagggtat ccatcttctt
    11461 tttgccattg atcacttcct gttttagcaa tttcggcacg cgcttcctca ataagaagca
    11521 taatttggtc aatttcattt ggaaaagcta atctgatttg cattatatta cccactttta
    11581 tatattatag actataataa cggtttaatt ttctttcgtg ataacattta ctatgtattt
    11641 tctgcttcaa atgttttcat aaaatcaatt aattcgatga caccttgttt aggaaaggca
    11701 ttataaaggc tagctctcat accacctacc gaacgatgtc ctttaatatt tttaaatcca
    11761 cgttctgtcg cttcctttac aaatttttca tctaaatctt tattaatagt tatgaaagga
    11821 atgttacata gagaacgact ctttttatct ctgacaggat tactataaaa ttctgaggaa
    11881 tcaatataat catagagaag tcctgatttt tcacggttag ctttttccat agcatcaaca
    11941 ccacctagac ttttcaccca ttcaaagact aatttagcaa tgtaaatact gtaagcaggc
    12001 ggcgtattat aaagagaacc agcatctgac tgaaccttat aatccaacat tgatgataaa
    12061 gttggttcct cattaatcat atcttcacgg ataataacta ccgttacacc agcaggtcca
    12121 atattttttt gagctcctgc ataaatcatt gcaaaatctt ctactttata ttttacagct
    12181 aatatattag aagacatatc cgcaataaca ggcactccat tagttttagg aatatcataa
    12241 agcgatgttc cttcaatggt attatttgtt gtcacatgaa cataggcagc tttaggatca
    12301 atctcttttt catcaaactc tggaatataa tcataaactg attcttctga tgaagctaat
    12361 agaattggtt caaaaggaat tgtttttgat agttttacag cctctgtata agcttttttc
    12421 ccccacgaac cagcaacatg atagtaagct ttacgccctc tggtaatatt caagggaatc
    12481 atactaaact gtaaggatgc accaccttgt aaaaagatta ctttataatt atccggaatt
    12541 cccatcaaat ctcttaagta acgctcagca cctttgataa tatcatcaaa ctctttggag
    12601 cggtgcgata cctccaaaac actcatacta gacccttgat aattaaggag ttcagattgg
    12661 gcttttacaa gcactggttt tggtaagact gcaggacctg cagaaaaatt ataaattatc
    12721 atataacacc tccatataat tgtgtaagat tatagcacag tattttgaat attgtaaaga
    12781 tttttttgat taattttgct ttttattcgt gatataatgg taaaggcgaa caatcaagtt
    12841 taaaggagta atttatgatt ttgagggaat tttgtgcaga gaatttaaca gacttaacca
    12901 gattagataa agctattatt tcacgtgtcg agctttgtga taaccttgct gtcggaggca
    12961 ctactcctag ctacggtgtt atcaaagaag ctaatcaata tttacatgaa aaaggtatta
    13021 gtgtcgctgt tatgattcgt ccccgtggag gaaattttgt atacaatgat ttagaattac
    13081 gtattatgga agaggatatc ctacgtgctg ttgagttaga atcagatgct cttgttttgg
    13141 gaatattaac ttcaaataat catattgata ctgaagccat tgaacaactt ctacctgcta
    13201 cccaaggttt gccacttgtt tttcatatgg catttgatgt tatcccgaaa tctgaccaaa
    13261 aaaaatcaat agatcaatta gttgctttag gctttacaag gattctccta catggttcat
    13321 ctaatggcga accaattata gaaaatatca aacatatcaa agcattagtt gaatatgcta
    13381 ataatcgtat tgaaattatg gttggcggcg gtgtgactgc tgaaaactat caatatattt
    13441 gccaagaaac tggggttaaa caagcccatg gtactagaat aactcaataa aaaattcgaa
    13501 acagggctat aagtttcttt agtatattta ctactaagtt ctctcatgtt ttgtaaaacc
    13561 tgaaagaacc ggaataagag cataccatct tttataacct actctcag
//
LOCUS       DATRSG010000057        13366 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRSG010000057 DATRSG010000000
VERSION     DATRSG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13366
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.057"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(18..248)
                     /locus_tag="VBD89_001596"
     CDS             complement(18..248)
                     /locus_tag="VBD89_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO7136113.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            546..2807
                     /locus_tag="VBD89_001597"
     CDS             546..2807
                     /locus_tag="VBD89_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7136114.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQAGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            2993..3448
                     /locus_tag="VBD89_001598"
     CDS             2993..3448
                     /locus_tag="VBD89_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7136115.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            3512..3814
                     /locus_tag="VBD89_001599"
     CDS             3512..3814
                     /locus_tag="VBD89_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO7136116.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTTAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(3884..6676)
                     /locus_tag="VBD89_001600"
     CDS             complement(3884..6676)
                     /locus_tag="VBD89_001600"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO7136117.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTRMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(6961..7731)
                     /locus_tag="VBD89_001601"
     CDS             complement(6961..7731)
                     /locus_tag="VBD89_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO7136118.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(7741..8529)
                     /locus_tag="VBD89_001602"
     CDS             complement(7741..8529)
                     /locus_tag="VBD89_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO7136119.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(8531..8785)
                     /locus_tag="VBD89_001603"
     CDS             complement(8531..8785)
                     /locus_tag="VBD89_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO7136120.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWLPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(8788..9393)
                     /locus_tag="VBD89_001604"
     CDS             complement(8788..9393)
                     /locus_tag="VBD89_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO7136121.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(9405..10079)
                     /locus_tag="VBD89_001605"
     CDS             complement(9405..10079)
                     /locus_tag="VBD89_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO7136122.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(10085..11365)
                     /gene="ftsZ"
                     /locus_tag="VBD89_001606"
     CDS             complement(10085..11365)
                     /gene="ftsZ"
                     /locus_tag="VBD89_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO7136123.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(11387..12760)
                     /gene="ftsA"
                     /locus_tag="VBD89_001607"
     CDS             complement(11387..12760)
                     /gene="ftsA"
                     /locus_tag="VBD89_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO7136124.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKTNIGERIRG
                     IFGSMFD"
     gene            complement(13033..>13366)
                     /locus_tag="VBD89_001608"
     CDS             complement(13033..>13366)
                     /locus_tag="VBD89_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HEO7136125.1"
                     /translation="DLLLLDMHDGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEV
                     GVYTTTSTIESTPVKAEDTKNKSTDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQ
                     APNPQNVN"
BASE COUNT         4182 a         2644 c         2216 g         4324 t
ORIGIN      
        1 aaggtctttt ataattatca gtataataaa tcgtaaattt ccattgcgat aaggtcaatg
       61 ctgtcaaatg aatatgattc acgcgcttct acatcacgta atgtgaaaac aggaccgttt
      121 tcagggtcat tactaaaaga tacttctgct acaacaacac cttcacgttc aaaattacgt
      181 tttaaattac caccatcagt cgccattaat tcgaggcgat tgatgattct cactaaatgt
      241 gattccatct tacttactcc tatatatttt ctagtctttt ctattttaac acaaaagtga
      301 ccaaagcaac atgaaaagca acatttttac taacttttta acgattttat cgtctaaaat
      361 ccgcaaataa cgatttttct cagtagtcat ctatttttca atttagcact ctaatatatt
      421 cagtgctaaa aacagaactt ttttcttgat ttctatgctt taggtgctat aataaaagca
      481 taaagtttga ctatatttga ctatatacac atcaatagtt aaatgtcata agaaaggggt
      541 agcctatgct ctgtcaaaac tgcaaactaa acgaatcaac tattcatttg tatacaaatg
      601 ttaatggaaa acaaaagcaa gttgatcttt gtcaaaattg ctaccaaatt attaaaactg
      661 accctaataa tccacttttt tcaggtttaa atcatgtttc acatgcgcct ggtggcatca
      721 atcctttctt tgatgatttc tttggagatt taaataattt cagagccttt aatggccaag
      781 atttaccaaa cactcctcct acacaagcag gaggaaaccg aggtggagga aacggtaatg
      841 gacgaaataa taatcgcaac caaactgcaa ctcctcctca agccaaaggg attctagaag
      901 aatttggtat caatgtcact gaaatagccc gtcatggtga tattgaccct gttattggac
      961 gtgactcaga aattattcgt gttatcgaaa tccttaatcg tcgtacaaaa aataatcctg
     1021 tacttattgg cgaacccggc gtagggaaaa ctgccgttgt tgaaggactt gctcagaaaa
     1081 ttgttgatgg taatgtgcct cataaacttc aaggcaaaca agttattcgt ttggatgttg
     1141 tgagtctagt acaaggaacg ggcattcgtg gtcaatttga agaacgcatg caaaagttga
     1201 tggaagaaat tcgtcaacgt caagatgtta ttctattcat agatgaaatt cacgaaatag
     1261 ttggagctgg tacagctggc gaaggtagta tggatgcagg taatatttta aaacctgccc
     1321 ttgcacgtgg agaattacaa ctagtaggtg ccactactct caatgaatat cgtattatcg
     1381 aaaaggacgc tgccttagaa cgccgtatgc aacctgtaaa agtcgatgaa ccttctgttg
     1441 aagaaaccat tactattttg aaaggtatcc aaaaaaaata cgaagattat catcacgtaa
     1501 aatataataa tgatgccata gaagcagctg cagtactatc taatcgttat atccaagacc
     1561 gctttttacc tgataaagca atagacttat tagatgaagc tggttctaaa atgaacctaa
     1621 cactaaattt tgttgatcca aaagaaattg atcaacgtct cattgaagca gaaaatttaa
     1681 aagcgcaagc gactcgtgaa gaagattacg aacgtgcagc ttacttccgt gaccagattg
     1741 caaaatataa agaaatgcag caacaaaagg tcgacgatca agatacacct attattaccg
     1801 aaaaaacaat tgagcacatc attgaagaaa aaacgaatat ccctgttggt gatttaaaag
     1861 aaaaagaaca atctcaatta attaatctcg cagatgactt gaaacagcat gtgatcggcc
     1921 aggatgacgc tgtcattaag attgcaaaag ctattcgtcg taatcgagtt ggtcttggta
     1981 gcccaaaccg tcctattggt tcctttttat ttgtaggacc aaccggtgtt ggtaaaactg
     2041 aactttctaa acaactagca attgagctct ttggttcagc tgatagtatg attcgttttg
     2101 atatgtcaga gtacatggaa aagcatgctg ttgctaaatt agtcggagcg cctccaggat
     2161 acgtgggata cgaggaagct ggacaactaa ctgaaaaggt tcgtcgaaat ccttactcgc
     2221 tcatccttct agatgaaatt gaaaaagctc atcccgatgt catgcatatg ttcttgcagg
     2281 tccttgatga cggtcgatta acagatggac aaggaagaac tgttagtttt aaagatacca
     2341 ttatcatcat gacctcaaat gctggttctg gtaaaactga agcaagtgtt ggctttggtg
     2401 cctcacgaga aggtaggacg aattccgtac taggtcaact aggtaacttc ttcagccctg
     2461 aatttatgaa ccgctttgac ggtattattg aattcaaggc tttagataaa gagaatctcc
     2521 tcaatatcgt tgatattatg ttatctgacg ttaatgcacg tctcgccatc aatggtattc
     2581 atctagatgt cactgataaa gtgaaagaaa aattggttga tttaggttac gatcccaaaa
     2641 tgggagcacg tccattacgt cgtaccattc aagaacatat tgaagatgct atcacagatt
     2701 actatcttga gaatccaagc gaaaaagaac ttcgtgctat tatgactagc aatggaaata
     2761 tcataataaa atcttctaaa aaaactgagg aaagtacaaa aggttgagta agaaactcaa
     2821 ccttttttca cactcttctc aaaaaataat agcctctata aagctgtttc tctcctatct
     2881 cgtctttaaa ttttaataca attattaagg aaatattcca aaaattttag tataataaat
     2941 aaaaaataat taaagctttt aaaatcataa aacacaatca ggagagagaa ctatgactaa
     3001 tcctactttc ggtgaaaaaa tagataatgt agactatagg tcacgatttg gtgtctatgc
     3061 tattattcca aatccaactc atgataaaat tattttagta caggcaccta atggtgcttg
     3121 gtttcttcca ggtggggaaa ttgaagaaaa tgaaaatcac ctagaagctt taactcgtga
     3181 gttaattgaa gaattaggtt attctgcaac aattggccat tactatggtc aagctgatga
     3241 atatttttac tctagacacc gtgatactta ctactataat cccgcctata tttatgaagt
     3301 tactgcctat cataaggagc aagcgccact agaagatttc aaccatctag cctggtttcc
     3361 tatccaagaa gctaaagaaa agctaaaacg aggaagccat agatggggtg tccaagcttg
     3421 ggaaaaaaat caccattcta ggaaataatt catacctttc ttcctaaata gtgttataat
     3481 aaacctaata agaatcaggg ggaaaatagt tatgagacta attaatacaa ccagtagtca
     3541 tcctgaacta gttcgaaatc aactccaaaa tacagatgca aaattagtgg aagtctattc
     3601 agctggcaac accgatgtcg tttttacaac agcacctaaa cattatgaat tattaatttc
     3661 aaacaaatat cgtgctatca aagatgaaga attagaagct attcgtgagt tcttcttaaa
     3721 gcgtaaaata gatcaatcta ttattattca agagcagatg aaatcactcc atactgccaa
     3781 gttaattgaa atttcatatc caacaacagc ataattaaaa gaagcctaat caaggcttct
     3841 tttttgatga ctactaaaca aatatagcat taaataattt ttattaagct tcaaaacctt
     3901 gagctactgc ttcaggataa tattgctcaa taattgctgc actagcatca caaacagcta
     3961 caccatacga acgcatcctt gtggttggat caatacggcg tgagcgttcg cagacttcac
     4021 cttcagcgtg ttcaacagta aatgcaacac cttcaaatga tactgagtca gctggtttgt
     4081 ctgcttcatc agcaattgtt aattgtgaga caatcatcaa gagtgcaata tcgctattta
     4141 acgctgttaa taacgttttc acttcttgac tagcataaat tgttaaatgt gcttctagag
     4201 atttaccaat gactttagcg ttacgtgctt cttcgagagc tttttgagct tgtgttcgta
     4261 acgtcatgaa tgctgaccac tcttcaagaa tttcttcttg accagagaat gtctgtgcaa
     4321 caggcatttc tgctaattga acaaattcct cctcctcatg ttcaaggtat gaccaaattt
     4381 cttcagcagt atgtggtaga atcggagtca aaagttttgt aagtttaact aaaatatcgt
     4441 agaaaacagt ttgcatacga cgtcgttctg gactgttagc cgcttcaatg taaaccacat
     4501 cttttgcaaa atcaaggtaa aatgctgata gatcaactgt tacgaagtta acgacagctt
     4561 tataaattgc cataaaatca taagcagcat aagctttgtt gattgtatcc acaacttgat
     4621 taaatttaat agtcatataa cgatcaacgg cacctaaatt ttcataggct actgcatctt
     4681 gtttaggatt aaaatcggaa gtatttgcaa tcaagaaacg caatgtattt ctgatcttac
     4741 gataagtttc tgatgtttgt tttaagatat ccattgaaat acgaacatca ttacttgaat
     4801 caacagaagt tacccacaga cgcaagattt ccgcaccaaa ttgtttttca acatcactcg
     4861 gaagaattgt atttcctaga gatttagaca ttttctcacc tttaccatca agtacaaaac
     4921 cttgagataa gactgctttg tatggagcgt gaccattaac agcaactgat gtaattagtg
     4981 atgagttgaa ccaaccacgg tattgatcag aaccttcaag ataaagatct gctggatagg
     5041 aaaggttttc acgagcattc attactccat tccatgatga accagaatca aaccaaacat
     5101 ccatgatgtc tgtttctttc tcaaagagac cattaggtga acctggatga gtataacctg
     5161 ctggtagaag gtccttagca tcacgttgcc accaaacgat tgagccgtat tcagcaaata
     5221 aatctgcaac gtgatcagtc acttcttttg tcatgatagc tgtaccatct tctgcataaa
     5281 aaattggaag tggaacaccc caagcacgtt gacgtgagat tacccagtcg ccacggtcac
     5341 ggatcatgtt ataaagacgt tttttacccc actcaggttg gaaatttgtc ttttcaattt
     5401 catcgaggat ttcttgacgg aatttcgaaa cagaggcaaa ccattgtgga accgcacgcc
     5461 aaatgattgg ctttttcgta cgccagtcaa atggatatga gtggttgata acttcgctgg
     5521 ctaaaagaag atctcccaat ttttctttta caagtggtgt cactttgtca tagaattgac
     5581 cttcaaaatc aggaccagca ttttccatca tcaaaccacg actatctacg gtaactacaa
     5641 catcaagtcc attagcaata ccaacgttat agtcatcttc accaaaacca ggagccgtgt
     5701 ggacaatacc tgtaccagaa tctgttgtaa catggtctcc aaggataacc aactcttcta
     5761 cttctgtatc ccatggatgt tctgtaacaa tgtgattaag ttctttacca gtgtgatgag
     5821 tcacaatttc aaagttttcc cagccaaact tagcagcgag actatctaca agaacctctg
     5881 caagaaggta tttacgctca ctacctactg gtgcaactac aacatactcc atatctggtc
     5941 ctactgttaa accgcgtgaa gctgttacag taaatggtgt tgtcgtccaa acgacgatat
     6001 aggtatctgt atcaagaatt cccttaccat ctttaacttt attggcatag tagagtgatg
     6061 tcgaatcaat atcatgatat tcgatttcag cctcagcaag ggcagactct gatgaccatg
     6121 accaatacac tggtttagca ccacgataga tatatccttt atctgccata gcaccgaaaa
     6181 cacgtacttg atctgcttca taatctggtg tcagtgtaat ataaggattt tcccaatcag
     6241 cagaaacgcc cagacgttta aaatcatcac gttgtttgtc aacttggctg agagcataat
     6301 cacgacacat ttcaaggtat tcagccaagt ccatttcttt acgtttaacc ccttttttag
     6361 ctaatacttg ctcaattggg agtccatgag tatcccaacc aggcacataa ggagctcgaa
     6421 aacctgacat tgattttgaa cgtacaataa tgtctttaga aattttattt agtgcatgtc
     6481 ctacatgaat attcccatta gcatagggag gtccgtcatg aaggtgaaag gctggttttc
     6541 cttcattcaa tgcttgacgt tttttataaa tatcagcttg atcccatgct tcttgccatt
     6601 gaggttcctt atttggaagc cctgcacgca ttggaaaagc tgtttgtcct aaattcaagg
     6661 tttcttttaa tttcatacta actccttatc tcatagtaat agttatcaca attttttaaa
     6721 ttgctaaata aaaaagcttt catcacataa aggacgaaag ctcgtggtac caccttaatt
     6781 cagagcagaa aatagctatt tcttactccc tcaacaaccc gtaaggtggg ttaagccttc
     6841 taccttactt aaataatagt tcaggtagaa tgattgaaaa tgataatttc aatattaggg
     6901 attgtagggc tctcaccatc tcctactcgc tgataatata atattaaaac cttgtttttc
     6961 ttaatcttca atatttaatt taaaagtttg agtttcacct aaattgatcg gttcttccat
     7021 ttggaaatta ttagtatcta ataaacctga ctcttcaagt tgtttgttac tttcttcaac
     7081 acgacgctgt aattcttcca tttcatctgg tgagaattga cgagtagcat caaatgactc
     7141 agtgtcatcc accactggta aagcatcatc ttcatcaaga actttttcaa caacttcttt
     7201 gaaagaagca tcagaatttt gaagataaat agctgttggt ttcaacaatt cttcccaagc
     7261 agatgagtta gctaatttta actgaccttc taattcagaa agtaagcgtt ggtggaaaac
     7321 acgtgattga cgtttcaaat cttctgtttc tatagcgaca cgcttagcat catctgtcgc
     7381 atcacgaagg atttgatttg cttttaattt agcctcatca attaaatgtt gagcatcaaa
     7441 tgtagctttc cccattaggt tagatgcttc atcctgtgct gaaattttca cacgttcagc
     7501 agtttcttga gctaaaataa ctgattgact taacgattcc ttcatttcgt tgaagtaagc
     7561 gattttctct tctaaatctt taatgtattg ctcttgctca cgattacgtc taatcaagtc
     7621 ttcgtaatcg tcaacaacaa tctctaaaaa ttcattaact tcttcttcgc tataaccgcg
     7681 aaattttgat gaaaatgttt tatctttaat ttcaagtgct gtaagtggca tgttttctcc
     7741 ttagttatgt atcattttgt ctacttctaa cttatatttt tgatttttag ttaaccctag
     7801 gttatggttc aacgtaaatc gaccatatcc tctaacacta atcaaatccc cctctactaa
     7861 ttgttcagaa actcgattga cgattgcata attgaccttg actttatctg cctcaatcaa
     7921 tttcgtcgac tgtgttcgtg aaatctttaa aatcgttgct aaaattttat caagacgtaa
     7981 actggaagca ataacagtta gtttttgaat atcttgtttg ggagttagaa gtttggacaa
     8041 gggaacttca gctaattgta cactagcagt tccaattttt gtaactgaat gaacaaggtg
     8101 atttgtcatt tgactatcga ccaaaacctg agcacagcct tcttcaacta ggatatcccc
     8161 taaaattgat cgttttacac ctaagtaatt cagtaaagtt cccataattt tagcatgggt
     8221 aaggtgatta aatttagcat tatactttat ttcaattagg ctaaggttaa aatcaaccat
     8281 atctaactga tagtattccg gtgctattat tactttaaca tattctgttt gaaaatactg
     8341 accagaagtg taataatgac tacctcgcct ctctagaaca ctttcgagta ttttaaattc
     8401 cctaggattt agaaattcag tggtaataaa cgaataggtg ttttcgacat attgagctaa
     8461 atggtctatt ttattgataa acgcatactc ttctggtcga aaatgctgat aaatatcatc
     8521 taacgtcatc ttatgtaaaa agcaagatta aaaatctact caacatattt agtaaaaaga
     8581 atgctactat tactgtaaaa tctagaccta aaaattgtag attaaatctt ctgagtgggc
     8641 gaataatcgg atcaacaatc tgtaccacta gtctaccaat ccatgaatcg tatgctcccg
     8701 gaagccaaga taacaaggca taggctagta agatataaga atagacccta attgttttta
     8761 ataatattaa tagtactaat atcatattta tcgtctcttc atatcaaaat caaaactcgt
     8821 ctcttgacca gtctttggaa tattcatctc ctcaatatcg accataacat tggctggtgt
     8881 taatagaaac attgaactcc caaccttttg taatgaacca tataatactc tacttgcacc
     8941 atcaatataa tccaagcaac gtcgtgcttg cgcatcaagc atatattgaa aatcaatcaa
     9001 gacacattca ttaacgataa gtaaatcaac aatttcttgt gcatcctcat acttacgggg
     9061 atacttcaga gcaattgtta cttgttctct ctgttgtcga actgagtctt tattttgaat
     9121 cctaggagta gcttgctgat aagcattgtc tctctgtacc ctttgtctct cctgacgatt
     9181 aggattatag gtagaagttc tcggacgact accaatcatc tcttcctctg cggagttagt
     9241 catatgacta cgctgagaag cttcctgtgc tgtagctgct ctggaatctc tttgcactga
     9301 agttctctct tgtacttcgt gtacttcatt ttcacttaca tcgtcagtgt caaaataaga
     9361 aataattttg tcaaatctat cttttaatgc catattcccc tccattattt aaagaatgaa
     9421 gttcctatcc ttacaaatgt tgatccattt tgaatagcaa tatcataatc tctactcatg
     9481 cccatgctta attctgtaaa cggcattcgt tctatgttcc tagcttgaag ccttttccta
     9541 agactctcag tctcttcaaa aattgatgat atttcctctt tagttgcttt taagggagcc
     9601 atagtcatga tgccgataag ttcaatttta tcgtaccgag aaattaagtt cagtgcatca
     9661 tctatttgct caatagtaaa accatgctta ctgtcttctc gtgatatatt aacttgtaga
     9721 aaacatttaa ctggtttttg agcacgtttt tgaatctcag ctgctaactt aacagaatct
     9781 aaagcatgaa aataatcaac ataattaatg acatctttaa cttttcgacg ttgtaaacta
     9841 ccaatgagat gccatgtaag cttttcatct tttaatgctt gatatttttc aagaaattta
     9901 tcaacacgat tttcaccgat atgattaaca cctgttctaa taagcgcttc tgttgtttga
     9961 cagttaacat attttgttac agctacgata tgaactgatt caggagcgcg acctgctttt
    10021 agtgctaatt tactaacatt gtcaaaaatc gctgttttat tttcttgtaa attcatcatt
    10081 ttaattaacg gtttttaaag aatggaggtg tttcaagttc atcatccata tcatcatttt
    10141 ctgaaaacgt tgacatagac aacttgctat caagttcacc ttctgtcgga cgtgaaatat
    10201 tatcacgacg aaggtcccaa ttaccaaaag cagaactttg ttgctgattt tgtgcatgag
    10261 gctgattttg ttgagttggc atttcgcgag actctgtcat atcaaagttg ccacgacgat
    10321 caaagtttga atttgaagta ctttgacgtt ctgaaggtgc ttgattagtt ggagctgatg
    10381 ttgtaaaacc agatacttga ttagttttat ctttacgcac acctgttgca acaactgtta
    10441 cgcggatttc gtctttcata tccatgtcaa ttgaagtgcc taaccaaata ttgacacctt
    10501 tacctgctgc ttggctaaca atctctgatg cttcctctgc ttcagtaagt gtcatatcca
    10561 taccgccggt aacattgaca ataacgtctt ctgctccatc aatcgttgtt tcaagaagtg
    10621 gtgaataaat tgcttttcga gcagcttcag taatacgttc ctcaccagaa ccgataccaa
    10681 ttcccatcag tgcattacct ttatttgcca taactgtttt aacatctgca aagtcaaggt
    10741 taattaaacc tgggttagta atcaaatctg taataccttg aacaccttga cgtaaaacat
    10801 tatctgcttc actaagagct tcaagcaatg gtgtcttctt atctacaatt tcaagaagat
    10861 tattatttga aatgataagc agtgtatcaa cttgttctct taattcttga ataccttcga
    10921 tagcgaagtt agaacgttta ttaccctcaa agccaaaagg acgtgtgata accgctactg
    10981 taagtgctcc taaactcttt gcaatacgtg caataactgg agccgcacct gtaccagatc
    11041 cgccccccat acctgctgtg ataaatacca tatcagcgcc agtaagtgct tctgttaaaa
    11101 cttcttcact ttcttcagct gctttacgac caacttcagg ttgtcctcct gcaccaagtc
    11161 cgcgagttaa ttttggacca agttgaatga cagtttcagc ttttgagcta cttaaggctt
    11221 gaatatcagt atttgctgca ataaactcaa cgccagcgac accttcgtca atcatgcggt
    11281 taatagcgtt accgccaccg cctccaacac caataacttt aataactgca ccttgtactg
    11341 atgctgtatc aaatgaaaat accattttta ttcctcactt attgttttag tcaaacatac
    11401 ttccaaaaat tcctctgatg cgctcaccaa tattagtttt aggctcagta ggctcttgag
    11461 cgttttctct tgctcggatt ggttctaaat tagaatcttc catagaagag gttaacggct
    11521 cagaataagg cattgtcgac attgtatttg ttttttcttt ataatcaaag tcgactggtt
    11581 tatgtcttag catttcgtcg ccagttacag catgttgtgc gataatgtca acttctgaca
    11641 tcattcctac ataatcaacg atactaataa cattagcgaa cattggattg cgaataccta
    11701 cttggtttgg aacgtgcaat ttaactctag ttccaaaaat ttgttgagca acctcgacaa
    11761 ctcctggcat aattgcacca cctccaacaa gaataatacc accaggtaaa tcaagtaaac
    11821 gacctctacc caagtcttgt ttgacatgtt ctaaaatttg acgaatacgt cctgaaataa
    11881 tttgcgaaag atagctttct gtaatttcaa caggttcctc gttaccaaca acattaactt
    11941 gaactgtatc agaagtactt gcttcttcaa cattagcttg tccaaagttg aatttcaatg
    12001 cttcggcaat ttctactgtt gtacgtaaca ctttagaaat atctttggtg acataatcgc
    12061 taccttcaga ataaatatta gtatattgca attcttgatt acgcatagaa gcgactgttg
    12121 tttgtccgcc tcccatatca atcacagttg caccaaactc acgttcacct tcgtttagaa
    12181 ctgattttgc taaggctaga ggggcaatca caacatgctc tacttttata cctgctcgtt
    12241 caacagtttt acgaagatta tgaaggatag ttgttggtcc agtatagata agaccacgca
    12301 tctcaaggcg aatccccatc atccctctag ggtctctaat cccttggaaa ccatctacaa
    12361 tgaattcaag cggaatcaat gaaataactt ctctttcagg agtaatgctt ttagtcaatg
    12421 ctgatttaac aacactttcg acatcttcat ccttaatttc ttttgattca ttgggaacag
    12481 gaatcattcc ttgagttggt tcaatttgga gaagatttgc tggtaatcca acattgattt
    12541 tgtcaatggt gatgcctgct ttctcctcag cttgttttac cgcctctttg attgcagttg
    12601 ctgctgcctc aatatcaata attataccat cttttacacc ggaactaggg acgttgctaa
    12661 ccccaattac attcatttca tttgcaataa attctgcaac taaaacttta atcgagcttg
    12721 ttcctatatc caaaccagta aaaaagccat ttctagccat tcactcgacc tcgctatctt
    12781 tccattcttt tctattctac atctgccaaa aacagaatta ttcagaaaat attataccat
    12841 aaaaaaagca gaattgttac caaattacgt ttttttatta agcaacatca aattgaaata
    12901 tttctaactt atttaaacca ttcgcaactt ataacaaact taaatttttt tcaagaaatc
    12961 aatttttact agcaatctat gactgacttg atatgtataa aaaagactga taaatccagt
    13021 ctttttttat cgctaattaa cattttgagg gttgggtgcc tgctctgttg ctatctgttg
    13081 tccttgttga ttagtatttg agttatttgt ttgtccttga ctattttccg caacctgacc
    13141 attttgtgtt tgtgttttat cagttgattt attttttgta tcttccgctt tcacaggggt
    13201 tgattcaata gtacttgttg ttgtgtaaac tcccacttcc atatcaacaa tagaaggttc
    13261 cttaaggttc ttcttaattt gtttgtaaaa aggaagtctt tctttaaatt tagataatgg
    13321 tattctaata ctatttccat catgcatatc taacagcagg aggtca
//
LOCUS       DATRSG010000058        13156 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRSG010000058 DATRSG010000000
VERSION     DATRSG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13156
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.058"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            58..813
                     /locus_tag="VBD89_001609"
     CDS             58..813
                     /locus_tag="VBD89_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7136126.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSDIANLFQEAEITIQSLLQVP"
     gene            932..1915
                     /locus_tag="VBD89_001610"
     CDS             932..1915
                     /locus_tag="VBD89_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO7136127.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQEDFQCLDEEAAVGEITCRFFNHSGDSVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTMYQKS"
     gene            2119..2442
                     /locus_tag="VBD89_001611"
     CDS             2119..2442
                     /locus_tag="VBD89_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO7136128.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            2459..2779
                     /locus_tag="VBD89_001612"
     CDS             2459..2779
                     /locus_tag="VBD89_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7136129.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            2781..4082
                     /locus_tag="VBD89_001613"
     CDS             2781..4082
                     /locus_tag="VBD89_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7136130.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSGVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            4263..6719
                     /locus_tag="VBD89_001614"
     CDS             4263..6719
                     /locus_tag="VBD89_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO7136131.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANTKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            6729..7397
                     /locus_tag="VBD89_001615"
     CDS             6729..7397
                     /locus_tag="VBD89_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7136132.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGCEASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            7465..8553
                     /locus_tag="VBD89_001616"
     CDS             7465..8553
                     /locus_tag="VBD89_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO7136133.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVIIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDVKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(8705..9631)
                     /gene="cysK"
                     /locus_tag="VBD89_001617"
     CDS             complement(8705..9631)
                     /gene="cysK"
                     /locus_tag="VBD89_001617"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO7136134.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSNDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(9722..10366)
                     /locus_tag="VBD89_001618"
     CDS             complement(9722..10366)
                     /locus_tag="VBD89_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO7136135.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            10422..11711
                     /locus_tag="VBD89_001619"
     CDS             10422..11711
                     /locus_tag="VBD89_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7136136.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKKNGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHIAEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            11711..12377
                     /locus_tag="VBD89_001620"
                     /pseudo
     CDS             11711..12377
                     /locus_tag="VBD89_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            12454..13008
                     /gene="raiA"
                     /locus_tag="VBD89_001621"
     CDS             12454..13008
                     /gene="raiA"
                     /locus_tag="VBD89_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO7136137.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT         4363 a         2166 c         2533 g         4094 t
ORIGIN      
        1 aagtttattg aacttgttta ataaataaaa aatgataaaa tagaaaggag gtgtttcatg
       61 aatagattag aaaatattat tagtttagta tcccagtacc aaaaaataga tgttaataca
      121 ctctcggagt tgttgcaagt ttctaaagta accatacgta aagatttaga taaactagaa
      181 ggcaaagggc ttttgcatag agagcacggt tatgcagttt taaacagtgg ggatgatctt
      241 aatgttaggt tatcttttaa ccataaaact aaaaaagaaa ttgctgcctt agctgcaaat
      301 atggtgtctg acaatgacac tatcttaata gaatcaggct ctacttgtgc tcttttagcg
      361 gaaaatatct gtcagacaaa gcgaaatgtc actattttga ccaactcttg ctttattgct
      421 aactatttac gagagtatga ttcatgccaa attgtcttac ttggtggtga gtatcaatca
      481 agttcacagg ttacggtagg tcctctcttg aaaaagatga tttcactttt tcatgtctcg
      541 ttagcttttg ttgggactga tggttttgat ccaaaactag gatttatggg tagagattta
      601 atgcgttctg aagttgcaca agagatggcg aatgctgccg atgaagtcat tattttaaca
      661 gattctagta agtttaatca gactgcattg gtggaacagc ttccgttgtc aactgtatca
      721 caagttatta cagataaaca tccaaattcg gatattgcta acttgttcca agaagcagag
      781 attaccattc aatctttatt acaagtacca taaaagaggc tatttttctt acattttgca
      841 catttgtggc gataatagat aaagtgtcat gtcaaattgc acagatgtaa cctttttgtt
      901 aaactgaagc aaatgctgaa aggttatgag tatgaaatta gagcgtcaaa aattgttagc
      961 taaagtagct tacctttact atatggaagg gaaaagccaa tctgagattg ccaatgaatt
     1021 aggaatttat cgtacaacaa taagtcgtat gttagccaaa gcgagagaag aaggtcttgt
     1081 tagaatcgaa atttcagatt ttaatccaga aatttttcag ttagaatcct attttaaaag
     1141 taagtatcac cttaaagata ttgagattgt ttcttcgcga aaagatagcg atacttcaga
     1201 gatagagaag gatttagcac atgtagctgc agctatgatt cgaaaaaaaa ttaaagagaa
     1261 tgacaaggtg gggattgctt gggggaggac acttagtaaa gtagttgaag caatgcgtcc
     1321 tcatccggtt tctcaagttt cttttgttcc tttggccgga ggtccaagtc atattaatgc
     1381 acgctatcac gttaatactc tggtttacga aatgtcaagg cgtttccaag gaagttgtac
     1441 tttcattaat gcaactctag ttcaagaaaa cgctaattta gcgaaaggaa tattaacttc
     1501 aaaatatttt gaggggctaa tggataattg ggaaaaactt gatgtggcaa ttgtgggcgt
     1561 tggtggtaaa cctaaaagca atgaacaaca gtggcttgat cttttaaatc aagaagattt
     1621 tcaatgcttg gatgaagaag cagctgtagg tgaaattact tgtcgttttt ttaatcattc
     1681 aggtgattct gtgaatcaac atttagctaa aaggacaata ggaattacct tagaacaact
     1741 acagaaagtt cctaatagaa tagcggttgc tcatggcaat tataaggctg cggcactctt
     1801 agccgtttta aaaaaggggt atattaatca tttagttact gatttttcca cagccttaaa
     1861 tattttaaga ttagataaag acacctttgt ggatactatg tatcaaaaga gctaggattt
     1921 attcctagct cttttaataa tgtttctatt accaagtgaa ttgttataga ataagcacaa
     1981 aagtgcgcta ttatcataaa gggcacaagg tctattgacg acgattaagg gacatgttac
     2041 atttgttttg cgaaagaaaa ttagaacctt ttgtaagaaa gtttaaactt ataaaaaatc
     2101 attaaagcgg aggtgcttat ggaaatcata gtcgcagacc aaattattat ggggctcatt
     2161 ttgaatgctg gggatgctaa acaacatatc tatcaagcct taaaacttgc aaaagaagga
     2221 aattttgcag aaagtaaaat tgaaatagaa ctagcagata gtgcactcct agaagctcat
     2281 aatcttcaaa cacaattcct tgcacaagaa gcaggaggga caaggacaga tatctcagca
     2341 ctctttattc attctcagga tcatttgatg acaagtatta ctgagattaa cttgattaaa
     2401 gaaattattg atttacgcca ggaattacat attcaaaaat aagtaaaggg gatcaaatat
     2461 gataaaaatt ggtttattct gtgcagcagg tttttcaaca ggtatgttag taaacaatat
     2521 gaaaatcgca gcggataaag aagggataga ggcacatatt gaagcttatt cacaaggaaa
     2581 aattgcagat tatgctaaag atttagatgt tgcattactt ggtccacaag ttagctatac
     2641 tctagataaa tcaaaatcaa tctgtgatga atacggtgta ccgatagcag ttattccaat
     2701 ggcagattat ggtatgttgg atggtgtaaa agttttgaaa cttgctttga gtttattgga
     2761 aacaagaaaa gaggcttgat atgtcaaagt ttgatagtca gaaaataatt actccgatta
     2821 tgaagtttgt caatatgcga gggattattg cactcaaaga tggcatgcta gcaattttac
     2881 cactaacagt tgttgggagt ctctttttaa tattagggca gcttccattt aaaggtctta
     2941 atcaagccat agctaatgtt tttggaccag aatggacaga accatttatg caagtttatt
     3001 caggaacttt tgcgattatg ggcttgattt cctgttttgc aattgcttat gcttatgcta
     3061 aaaatagtgg tgtagaaccg ttacctgctg gcgttttatc actttcgtct ttctttattt
     3121 taatgaaatc atcttatatt cctgtaaaag gagaagcaat agcggacgct atttctaagg
     3181 tttggtttgg cggtcagggg attattggag caattattat tggcttagta gttggtgcta
     3241 tctatacatg gtttatccaa catcatattg ttataaaaat gccagagcaa gtaccacagg
     3301 caatagcaaa acaatttgaa gctatgattc cagcttttgt tatctttctt ttatcgatga
     3361 ttgtttattt gattgctaag gtaacaactg gaggtacctt tattgagatg atttatgata
     3421 tcattcaagt accgttgcaa ggcttaacag gctcacttta tggagcaatt gggattgctt
     3481 tctttatttc attcttatgg tggtttggtg tccatggcca atctgtggtg aatggtattg
     3541 tgactgcttt gttactatcc aatttagatg ccaataagtc tttattagca gcaaatcgct
     3601 taacattaga taatggcgct cacattgtaa cacagcagtt tttggatagt ttcttgattt
     3661 tatcgggctc aggaataact tttggactag ttattgcgat gctttttgca gcaaaatcaa
     3721 aacaatacaa ggcacttggg aaagtggccg cttttcccgc aattttcaat gttaacgagc
     3781 caatcgtctt tggctttcca atcgtaatga atcctgtgat gtttctgcct tttattttag
     3841 taccagtttt ggcagcttta attgtttatg gagctattgc ggttggtttt atgcaaccat
     3901 tctcaggtgt tactttacca tggagtactc cagcaattat ttctggattc atggtaggtg
     3961 gttggcaagg tgcactcgtc caaatagtca ttttagctat ctcaactgct gtttacttcc
     4021 cattctttaa aatccaagat aatattactt acaaaaatga atgtgaaatg gaaaggggat
     4081 agctagcaga taagaggttg aggtgtgtaa tgcaccttaa cctttttcta aaaatgcaca
     4141 tttgtgcaac tttagatgta gcattctatt ctttattttc ttcttgttat tttaggattt
     4201 aggtgctatt atatttacga agaaaaactt tttagtttcg aaagaaacaa gcggaggctg
     4261 ttatgaccaa acaattactc actcaaaaat atacaaatga tgttcaacaa aattctcaaa
     4321 agcattttgg ctatttgaca gagcgcatgt attcttatcg cgataaggtt cttgataaga
     4381 aaccattcat agatgcagaa cgtgctatct tggtaactga agcctatcaa aagcatcagg
     4441 aaaaacctaa tgttttaaag agagcttaca tgttgcaaaa tattttggaa aaaatgacaa
     4501 tctatattga cgatgagaca atgattgtcg gtaaccaagc ttcatctgat aaagatgctc
     4561 ctatttttcc agaatacact ctagaatttg ttgtaaatga actagacctt tttgaaaaac
     4621 gagatggtga cgtcttttac attacagaag aaaccaaaga acaaattaga aatattgcac
     4681 ctttctggga aaataataat ttacgggctc gtgcgggcgt tatgcttcca gaagaagttc
     4741 aagtttatat ggagacaggc ttctttggga tggaaggtaa gatgaattct ggggatgctc
     4801 acttagctgt taactatcag aagttactag aagaagggct tattggcttt gaaaaaaaag
     4861 caagaaaagc taaagctgac ttagatttaa caaagcctga gagtattgac aaatatcatt
     4921 tctatgattc tattttaatt acaatagaag ctgttaaaac ctatgcagag cgttttgcta
     4981 tcctagcaaa aaaacaagct aaaacagcaa atactaagag acgtcaggaa ttgttagata
     5041 tcgctagtat ttgtgaacga gtgccatatt acccagctga aacgtttgca gaagctgttc
     5101 aatcggtttg gtttattcag tgtattcttc aaatagaatc aaacggtcat tctctatctt
     5161 atggacgctt tgatcaatac atgtatccat atgttaaagc tgacttagaa gctggacgtg
     5221 agactgagga ttcgatcgtt gagcgcctaa ctaacctatg gattaaaaca ataactatta
     5281 ataaggttcg cagccaagct catacgtttt cgtctgctgg tagtcctctt taccaaaatg
     5341 taacgattgg aggacaaacc cgacataagg aagatgctgt taatccttta tcatttctag
     5401 tactaaaatc agtcgctcag acacacttac cacagcctaa tctaacggtt cgttatcatg
     5461 ctaacctaga caagtcattc atgaacgaag ctattgaagt tatgaaatta ggttttggta
     5521 tgccagcttt taataatgat gaaattatta ttccatcatt tattaaaaaa ggagtttccg
     5581 aagaagatgc ctatgattat tcagcgattg gttgtgttga aacagcagtt cctgggaaat
     5641 ggggttatcg ttgtaccggc atgagctata tcaacttccc taaagtgtta ttaattacaa
     5701 tgaataatgg cattgacccg gcttctggga agagatttgc gccttcttat gggcatttca
     5761 cccagatgac ctcatataaa gaacttaaag aagcttggga caaaacactt cgttacttaa
     5821 ctcgaatgag tgttattgtt gaaaatgcta ttgatattag tctcgaaaga gaagtacctg
     5881 atattctttg ttcagcttta actgatgatt gtattggacg aggtaagcat ttaaaagaag
     5941 gtggtgcagt atatgactac atatctggtc tacaagttgg aatcgctaat ctttcagact
     6001 cacttgctgc gcttaaaaaa ttagtatttg aagaaaaacg cctaaccaca ttagaagtat
     6061 ggcaagcatt gcagagtgat tacgcaggtc cgcgtggtga agaaattcgt caaatgttga
     6121 tcaatgaagc accaaaatat ggaaatgatg atgactacgc ggatagttta gtacgtgagt
     6181 gttatgatgt ttatgttgaa gaaattgcta aatatcccaa cacacgctat ggacgtggac
     6241 caatcggtgg tattcgctat tcaggaacgt cctctatttc agcaaatgta ggtcaaggac
     6301 gaggtacttt agcaactcca gatggtagac atgcaggaac tccattagct gaaggttgtt
     6361 caccatctca taatatggac aaaaagggac ctacctctgt tttgaaatca gtttcgaagt
     6421 taccaactga tgagattgtc ggcggtgtac tccttaacca aaaagtcaac cctcaaacct
     6481 tggcaaaaga agaagataaa caaaaattaa ttgccttact tagaacattc ttcaatcgcc
     6541 ttcatggtta ccatatccag tataatgttg tctcacgtga gactttgatt gatgctcaga
     6601 agcaccctga aaaacaccgc gatttgattg tgcgtgtagc aggttattct gcattcttta
     6661 atgtcctatc taaggcaact caagatgata ttattgcacg tacagaacat gctctttagg
     6721 agattattat ggaattttta cttgacactt taaatttaga agctataaaa aaatggcatc
     6781 atatactgcc actagcaggt gtaacttcaa atcccaccat tgctaagaaa gaaggagata
     6841 tacatttttt ccaacgcatt cgtgatgttc gagaaattat tggatgtgaa gcaagtttgc
     6901 atgttcaagt agtggccaaa gattatcaag ggatactgga tgatgctgct aaaatacgcc
     6961 aagaaacgga tgatgacatt tatatcaagg ttccagttac gccagatgga ttagcagcaa
     7021 ttaaaacgtt aaaagcagaa ggttataata ttacagccac agcaatatac accagtatgc
     7081 aaggtctttt ggctatatca gctggagcag actacctagc accttacttt aaccgaatgg
     7141 agaaccttga tattgatgcg acacaagtta ttaaggaatt agcgcaagca atagagagga
     7201 caggttcttc tagcaaaatt ttagcagcta gttttaaaaa cgcttcacaa gtaacgaagg
     7261 ccttatcaca gggtgctcaa tcaatcaccg cgggaccaga tatttttgaa tcagtctttg
     7321 cgatgccatc tattgcaaaa gctgttaacg actttgcaga tgattggaaa gccagtcaac
     7381 actcagaaca tatttaacag agaacgccaa cttttgaggc tgtatcttta acataaaatt
     7441 ttttaaagaa gaaagaggtt gagaatgaaa gttttcgcaa gtccatcgcg ctatatacaa
     7501 ggaaaggatg ctttattcca aagtattgaa catatcaaat ctttaggtca gactccactt
     7561 atcctttgtg atgatgttgt ttataatatc gttggagaaa ggtttttatc ttatctacaa
     7621 gacgatttgt taccacatcg tgtctccttt aacggagaag catctgataa tgaaatcaat
     7681 cgtgtcgttg ctgttgcaaa agaaaagaat agtgacctta ttattggact tgggggtgga
     7741 aaaactattg actctgcaaa ggcaattgca gataaagtta atttaccagt gatcattgca
     7801 ccaacagtag cttcaacaga tgccccaaca tcagcgttat cagtaatcta tacagatgaa
     7861 ggagcttttg aaaaatatat tttttattca aagaatccag acttagtttt ggtcgatact
     7921 caagtcattg ctcaagcgcc aaaacgttta ctagcttctg ggattgcaga tggcttagct
     7981 acttgggttg aagcgagagc agtcttacaa aaaaatggga tagcgatggc tggcggtaga
     8041 cagactttag cgggcgtggc aattgctcaa gcatgtgagc gtactttatt taatgatagt
     8101 ttacaagctt tagcggcttg tgatgtcaaa gtggttacaa aagctttaga aaatgttatt
     8161 gaagctaata ccttacttag tggtttaggt tttgaaagcg caggattagc tgcagctcat
     8221 gctattcata atggttttac tgccttgagt ggagatattc atcacttaac ccatggtgaa
     8281 aaagttgcct atggaacgtt gacacaactt ttcctcgaaa atagaccaaa ggaagaaatt
     8341 gatcgctaca ttaatcttta ccaagcgatt ggaatgccaa ctacacttgc agaactccat
     8401 ttgggtgatg caacatatga agaactttta aaagttggtc agcaagcaac aattgaaggt
     8461 gaaacaatac atgaaatgcc attcaaaatt agtgcagagg atgtcgctgc agccctgtta
     8521 gcagttgacc gctatgtttc aaatcatcaa taaaaacaat agaaccaagt gaaattgctt
     8581 ggttctattt gtcgttttaa tattttaact aaatagggct tactagtatt taaagagata
     8641 taaactaaaa aagatcagac aacatatcta atctttttta gttatcgttt actgaagatt
     8701 aaagttattc aaaatcatac aggcttgttg ataagtaacg ttcgccatta tcagggagaa
     8761 gagctaaaac ctttttccct tttcctagtt gtttggccac ttcaatagca gcataaatag
     8821 cagctgaagc agaaattcct gcgaggaaac cttctagtcc gccgatgata cgtcctgttt
     8881 caatagcatc gttagatgta acgcgaatga tatgatcata agaatctgta gccaatgttt
     8941 ctggaatgaa acctgcagaa atcccttgta ttttatgagg cccaggttgc tcaccagaaa
     9001 ggattgcaga ttcatcagct tctacagcat aaatagcgac atcaggattg acttttttta
     9061 atgcacgaga gacaccagta attgtcccac cagttcccac acctgcgata aaagcatcta
     9121 aacctttttc accaaaagtc tcgataattt ccctaccagt cgttttttcg tggatttctg
     9181 gattggcttg attattaaat tgcaggggta gccaagcatt ttgctctgca gaaatttctt
     9241 ttgccttagc aatagctccc ttcatccctt cactaccagg tgttagtact aattgagcac
     9301 cataggcttg aatgatttta cgacgttcaa ttgacatggt ttctggcata acaataataa
     9361 cattataccc tttagctttt cctacccaag ccaaaccgat acctgtatta ccagatgtag
     9421 gttctacgat tgtatctccg gctttaagaa tccctttttg ctctgcatct tcaatcatag
     9481 ctagagcgat acgatctttg acagatgaac ctgggttaaa ggattctaac ttaacgtaca
     9541 cctctgcagc tccttctgga acaatatgat gtaattgaat aatgggtgta tttccgatta
     9601 agtctgtaat agaattataa atttttgaca taagctatac ctcttcatga cattggttaa
     9661 gaatattata aaactcacat tgattaaagt aaaatatcaa ttctttatca agtgatagct
     9721 attatttatc ggtttcagcc ttaaagggaa tttcaacaat ttgactccct tcttcagtta
     9781 acaaagcttt tccttggtag aactctgtca aattagttat tgtgtgttca aattccttag
     9841 ggtcaacgta tattttacag gttacagctt ctaaaaattc tgtttcaaac tcctgaaggt
     9901 ggtgctcttt taggaagttg tcaaaagttt gatattgagg ataagttaat tgaatacgaa
     9961 taccaatttg ttctttaact tctactaccc cgatctcttt gatggcatta gctacgctac
    10021 cagagtaggc acgaataagc ccccctgtgc ctaatttgat acctccaaaa taccgtgtaa
    10081 cgacggcaac aacatttgtc aggccttgct tttcaagtac ggttagcata gggatacctg
    10141 ctgtaccact aggttcacca tcatcactag atcgttttat atcccctttt tcgccaataa
    10201 ccatagctga acacgaatga ttggctttgt agtgcgcttt cttgatttgc gtaatataat
    10261 tacgcccctc ctcttcggac tctactcgct ttagatgaca aataaatcgc gattttttaa
    10321 tttcttcttc gacaattccg tctgatttta tcgttttata gttcatagag aaattttaca
    10381 aaaattcttc aaatttagca aaaaaatccg aatgaaataa tatggaaaat tatttaggta
    10441 gactttggac aaaagcacaa ttatctgagc aacttagaaa aattgcaatt aacttgccat
    10501 cttttataaa gaaaggaagc gactatattt gtactcgatg ctctagttct gttgccaaaa
    10561 attgccaatt accaactgga aattattatt gtagagaatg tatagttttt ggaagagtta
    10621 ctagtaatga aaatttatat tatttcccac aaaaaacatt ttctaaaacg aattctttaa
    10681 aatggaaagg gggactgaca ccttatcaaa atgaggtttc agaagagttg ttgaaaggta
    10741 tttcaagtaa ggaaaatctt cttgttcatg ctgtgacagg ggctggtaaa acagaaatga
    10801 tataccactc tatcgccaaa gtaattgata ctggagggag cgtttgtatt gccagtccta
    10861 gaattgatgt ttgtttagaa ttgcataagc gactttcaaa cgattttaga tgtgccataa
    10921 cattaatgca tggagaatct ccttcttatc agagatcccc tttaactatt gccacaacac
    10981 atcaattgtt aaaattttat catgcttttg atttgctaat tgttgatgaa gttgatgctt
    11041 ttccatatgt cgacaacccg atactatacc atggtgtaaa acaggcctta aagaaaaacg
    11101 gaacaagtat ttttctgact gcaacttcaa cgactgagtt agaaagaaaa gttgcaagaa
    11161 aagaactaaa aaaattacat ctagctagga gatttcatgc caatccgtta gtgatacccg
    11221 agatggtttg ggtaagtggt atccagaaaa gcttgcagac acaaaaacta cctcccaaat
    11281 tatatcagct gataaataaa cagcgacaaa cgagatatcc tttgttatta tttttccccc
    11341 atattgcaga aggtcaggta ttcaccgaaa tgttaagaca agcctttcct atggaaaaaa
    11401 ttggttttgt ttcaagtaag agcacatcta ggttaaagtt agtgcaagat tttcgagata
    11461 acaaattgtc tatcctagta tcaactacca ttctagagag aggagtaact ttccctagcg
    11521 tcgacgtttt tgttatacaa gcaaatcatc atttgtttac gaaaagttcc ttggtacaaa
    11581 tttcaggaag agttggaaga gcattagaaa gacccgaagg gctcctttac ttccttcatg
    11641 atgggaaaag taaatcgatg caccaagcca taaaggagat taaaaacatg aatcatatag
    11701 gaggatttta atgacttgtt tgttatgtca tgaaatagat ttatcgcaat taacttttgt
    11761 ggaacttata cttctaaaac caaagcaaaa cgtaatttgt caaacgtgca agggtagttt
    11821 tgaagcactt agtagggaga tgggttgcca aacatgttgc aagcaaatcc tccgaaaaca
    11881 atgtcaagat tgtatttatt ggggtaaaaa aggtatagag gtaaatcatt tttcccttta
    11941 tagatacaat gaagcaatga aaaaaatatt tttctctttt taaatttcaa ggggattatt
    12001 tgttgaaaga tgtttttaca aaagaaataa aggctgctct aaaaaggtat aagggctaca
    12061 ctatagtgcc agttcccttg agtcatgaag gataccaaaa taggcaattt aatcaagtga
    12121 ttgcttttct acaatcggca aatatacctt ataaaaatat tctttctaaa aaagatggag
    12181 gtaagcaatc ggctaataat aaagaagaaa gactcaaaca agttcagcaa tttacattaa
    12241 aaaatgaggc tgagttaagg gataaccttt taattgttga tgatatttat acaacaggcg
    12301 caacaatagc acaaatcagg aaactattag aagaaaaagg tataaaaaat ataaaaagtt
    12361 tttcattagc acgctaaaat cttgcaaatt ttcatgaaag cggttataat aagaatatag
    12421 aaagcgttaa caacgcaaga aagaggtact catatgatta aatacagtat tcgtggtgaa
    12481 aacatcgaag taacagaagc tattcgtgag tatgttgaga ccaaactctc taaagttgaa
    12541 aaatacttta atgaagaaca agaattagat actagagtca acctcaaagt atacagagag
    12601 aagaccgcaa aagttgaagt aacaatctta atagattcta ttactttacg agcagaagat
    12661 gtttcacaag atatgtatgg gtcaattgac ttagttgttg ataaaattga gcgtcaaatt
    12721 cgtaaaaata aaactaaaat tgcgaaaaaa tatcgtgaaa aaattccagc aagtcaagtt
    12781 ttcacaacag aatttgaggc tgagccagat gaagaagcag taagccaaag aattgtacgt
    12841 actaagaatg ttaatctaaa accaatggat gttgaggaag cattgttgca aatggaactt
    12901 ttaggtcatg atttctttat ctatacagat gcagaagata atacgacaaa tgtcctttac
    12961 aagcgtgaag atggcgaact aggtttaatt gaagctaaag gaaattaact ttgtaacata
    13021 atttaaaaaa tctcagagtt ttttctgaga tttttttaaa acctattgac aacaaataaa
    13081 aaattatagt atactaaatg agttcttaag cgaacctaat attgtattat cattttataa
    13141 acaacttaaa aaaagt
//
LOCUS       DATRSG010000059        13045 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRSG010000059 DATRSG010000000
VERSION     DATRSG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13045
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.059"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            313..702
                     /locus_tag="VBD89_001622"
     CDS             313..702
                     /locus_tag="VBD89_001622"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO7136138.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            767..1816
                     /locus_tag="VBD89_001623"
     CDS             767..1816
                     /locus_tag="VBD89_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7136139.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            1961..3496
                     /locus_tag="VBD89_001624"
     CDS             1961..3496
                     /locus_tag="VBD89_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136140.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            3489..4550
                     /locus_tag="VBD89_001625"
     CDS             3489..4550
                     /locus_tag="VBD89_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136141.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFQLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            4552..5508
                     /locus_tag="VBD89_001626"
     CDS             4552..5508
                     /locus_tag="VBD89_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136142.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            5714..7084
                     /locus_tag="VBD89_001627"
     CDS             5714..7084
                     /locus_tag="VBD89_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO7136143.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(7205..8194)
                     /locus_tag="VBD89_001628"
     CDS             complement(7205..8194)
                     /locus_tag="VBD89_001628"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7136144.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            8433..10892
                     /gene="gyrA"
                     /locus_tag="VBD89_001629"
     CDS             8433..10892
                     /gene="gyrA"
                     /locus_tag="VBD89_001629"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO7136145.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            10899..11642
                     /locus_tag="VBD89_001630"
     CDS             10899..11642
                     /locus_tag="VBD89_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO7136146.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            11658..12071
                     /locus_tag="VBD89_001631"
     CDS             11658..12071
                     /locus_tag="VBD89_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7136147.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            12224..>13045
                     /locus_tag="VBD89_001632"
     CDS             12224..>13045
                     /locus_tag="VBD89_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO7136148.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKD"
BASE COUNT         4229 a         2004 c         2694 g         4118 t
ORIGIN      
        1 agcgtaaaaa taataaatag ttcggataat aactatcgta ttattttttg gaaaggtttt
       61 ctctaaaaat gttttcttta aattccgtat aagataaaaa gaattctgct ttttattctg
      121 aaaattataa aaaactactt acattatcta aaaaaaatgg taaaatagat tcagctaaaa
      181 taccaagact aatgaaacta caagacttgg ttactcaata ataaatactt ctgtgtttga
      241 taagaagata ataaacgtgt tctaaaaatt ttgccagtaa acattatgaa gcgaaaggtg
      301 cagctactaa aaatgggaaa tatagagttg aaaaaattgg ctgtaaaagc gagtgaaaat
      361 gcatacgttc cttactcgaa atttccagta ggcgcagcat tgcgtacagc agaaggaaaa
      421 atatttacag gatgtaacgt tgaaaatatc agttatggat tagcaaactg tgctgagcgt
      481 actgcaattt ttaaagctgt ttcagaaggt tataaagatt tttcggaaat tgctatttat
      541 ggaaatactg aagaaccaat ttctccttgc ggtgcatgcc gtcaagtgat ggtggaattt
      601 tttaataaga atgctaaagt tacattgatt gctaaaaatg gtaagacggt cgagacgacg
      661 gtcggtgagt tgttaccata ctcgtttgtt gatttaaaat aatttaaatc agttgctctt
      721 ttaagcattt agcttaaata ttaagttttt taggagggtt catttcatga acaaaaaaat
      781 tgccgggatc ggcttggctt cgattgcagt acttagttta gctgcatgtg gacatcgtgg
      841 tgcttctaaa tctggtggta aatcagatag cttgaaggtt gcaatggtaa cagataccgg
      901 tggtgttgat gataaatcat ttaaccaatc tggttgggaa ggtatgcaag cttggggcaa
      961 gaagaatggc cttaaaaaag gagctggttt tgactatttc caatcggcaa gtgaatctga
     1021 ttatgcaact aacttagata cagctgtgtc tagtggttat aaattgattt tcggtattgg
     1081 attttctctt catgatgcta ttgataaagc agcagacaat aacaaagatg ttaattacgt
     1141 catcgttgat gatgttatta aagggaaaga taatgttgca agtgttgtct ttgcggataa
     1201 tgaatcagct tacttagcag gtattgcagc cgctaaaact accaaaacaa aaacagttgg
     1261 ctttgtaggt ggtatggaat ctgaggttat tacccgtttt gaaaaaggtt ttgaagcagg
     1321 tgtcaaatca gttgataaat caattaaaat taaagttgac tatgctggtt cattcggtga
     1381 tgctgctaag ggtaagacaa ttgcagccgc acaatatgct tctggcgcag atattgttta
     1441 tcaagtagct ggtggaactg gtgcaggtgt attcagtgaa gcaaaatcac gcaatgaatc
     1501 tttaaaagaa gctgacaaag tttgggtatt aggtgtagac cgtgaccaag cagcagaagg
     1561 taagtatact tctaaagatg gtaaggcttc taactttgtt ttggcttcat caattaaaga
     1621 agttggaaaa tcagttgaat tgattgctac taaaacaagt aaaggtaaat tcccaggtgg
     1681 taatgtaaca acttatggtt tgaaggatgg tggagttgat atagcaacta caaacctttc
     1741 agatgacgcc gttaaagcga ttaaagaggc taaagctaaa attatttcag gagatatcaa
     1801 agttcctagc aaataatatc tttaagaact ttcaaaaaag cgattagaaa tcgctttttt
     1861 gaagctgaga taaacttata tctcagtaaa atccatcagt cgttaacgat tggtggattt
     1921 tactgaacta taaatgaatt tttctgaaag ggagaaccac atgacacaga atgtcattga
     1981 aatgaaagaa atcactaaga aattcggtga ttttgtggca aatgatcata tcaatttgac
     2041 ggttgaaaaa ggtgagatac atgctctgct tggcgaaaac ggtgcaggga aatcaacttt
     2101 aatgaacatg ttagcaggtt tactagaacc aacagacggt caaattttta ttaatggtca
     2161 gcctgtaact attgattcac cttccaaatc gtcacaattg gggattggta tggtacatca
     2221 gcatttcatg cttgttgaag catttactgt agctgaaaat attgttctag gaaatgaaac
     2281 cacccaaaat ggtgttttag acattaagac agcagctaaa gaaataaagg aactatcaga
     2341 gaaatatggt ttgtcagtaa atccaaatgc taaaatatct gatatttctg taggagcaca
     2401 gcagcgtgtt gaaattttga agacacttta tcgcggtgca gatattttaa tttttgatga
     2461 gccgacggcg gtgcttacac catcagaaat aaaagaattg atgacaatta tgaagagcct
     2521 tgttaaagaa ggcaaatcca ttatactaat tacccataaa ttggatgaaa ttcgggcagt
     2581 tgctgataaa gttactgtga ttcgccgtgg taaaagtatt gaaacagtcc ctgttgcagg
     2641 cgcaagttct caacaactgg cggaaatgat ggttggacga tctgtttcct tcagaactga
     2701 aaaaaaggaa gctaatccga ctgacataat cctttctgta aaggatttag ttgtagaaga
     2761 aaatagaggc ggcgttctag ctgttaaaaa tctgtcgtta gatgttcgag caggtgaaat
     2821 tgtaggtata gctggtattg atggtaatgg acaaagtgaa ttaattcaag ctattacagg
     2881 tttaagaaag gtaacttctg gtcaaattgt aattaaaggt aaagatgtca caaaattttc
     2941 aagtcgacaa attacagaat taagtgtagg tcatgttcca gaagatcgtc atagagatgg
     3001 tttggttcta gatatgacga tggcagaaaa tttagcttta caaacttatt ataaagagcc
     3061 attgagtcat aaaggtatcc ttaattttgc taaaattaaa gagtatgctc gtcaattaat
     3121 gacagaattt gatgtgcgtg gtgctggtga acatgtttta gcaagaggat tttctggtgg
     3181 taaccaacaa aaagctatta ttgctcgtga agtggatcgc gatccagacc tactcattgt
     3241 tagccaaccg actcgtggtc tagatgttgg tgctatcgag tatattcata aacgtttgat
     3301 tgaagagcgc gataaaggga aagcggtctt agtagttagc tttgagttag atgaaatctt
     3361 aaacttatca gatcgtattg cagttattca tgatggtaaa attcaaggaa ttgttaaacc
     3421 cgaccaaacc aacaagcaag aattgggtat tttgatggct ggtggaaaaa ttgaaaagga
     3481 ggagagagat gtctaagaag gcgcaaaaaa ttgcagttcc tctgatttca gttgtactag
     3541 gaatcattct aggagctatt attatgctga tatttggtta tgatccactt tggggttatg
     3601 agggattgtt ccaaacagca ttcggtagta taaaaaatat tggtgaaatt ttccgtgcaa
     3661 tgggaccatt aattttaatt gctctaggtt tctcagttgc cagtcgtgca ggtttcttta
     3721 atattggact tccagggcaa gccttatcag gatggattgc cgcaggttgg ttcgctcttt
     3781 cacacccaga tatgccacgt ccagcaatga ttctttgtac tattattatt ggtatcgttg
     3841 ctgggggaat cactggagct attccaggta ttctcagagc ataccttgga acaagtgaag
     3901 ttattgtaac tattatgatg aactatattg ttctttattc aggaaatgct attgtacaac
     3961 gtgttttccc aaaaagcatt atgcgtacaa gtgactcgag tgtatatgtt tctgccaatg
     4021 catcatacca gacagattgg ttatctagtt taacgaataa ttcacgtatc aatattggta
     4081 tcttcattgc tattattgca gttgtgttag tttggttcca attaaataaa acaacgcttg
     4141 gatttgaaat tcgttcagtt ggtttaaatc caaatgctag tgagtacgct ggtatgtctg
     4201 caaagcgtac aattattcta tcaatgatta tttctggagc ttttgcagga cttggtggtg
     4261 ttgttgaagg actaggtaca tttgagaatg tatttgtaca gccatcctcg ttagctattg
     4321 gctttgacgg tatggcagta agtctactcg cagcgaatag tccaataggt attttatttg
     4381 cagcatttct atttggtgtt ctaagtgttg gtgcgcctgg tatgaacatt gcaggtattc
     4441 cacctgagtt aattaaagtt gtaacagctt caatcatctt cttcgtaggt gtccattaca
     4501 ttattgagta tgttattaaa cctaaaaaac aaatgaaagg tggtaagtaa attgagttta
     4561 acaacgattt ttgcattgct attttcatca atgctaattt acgcaacacc tcttatcttt
     4621 acaagtattg ggggaacctt ctctgaacgt ggtggtatcg tcaacgttgg tttagaagga
     4681 attatggtaa ttggagcttt ctcaggcgtt gtatttaatt tagagtttgc ttctgttttt
     4741 ggtgatgcga ctccatggat ttctgtacta gttggtggtt tagttggttt aattttttct
     4801 gttatccatg cagttgcgac agttaatttt cgtgcagatc atattatcag cggaaccgtt
     4861 cttaatttaa tggcaccttc attagctgtt ttcttgatta aggttcttta taataaaggt
     4921 caaactgata atattcaaga atcatttgga aaatttaatt tccctatact atcagatatt
     4981 ccttttgttg gtgatatctt ctttaaagga acaagcttag tcggttatat tgctattctt
     5041 ttctcattcc ttgcttggtt tattctatat aaaactcgct ttggattaag attacgctca
     5101 gttggagaac atcctcaagc agcagacacc ttaggtatta atgtttatct tatgcgttac
     5161 tctggtgtct tgatttcagg tttccttggt ggaataggag gagctgtata tgctcaatct
     5221 atttctgtta actttgcagc gacaactata ttaggaccgg gctttatctc cttagcggcg
     5281 atgatttttg gtaagtggaa tccaattggt gcaatgcttg ctagtctatt ctttggttta
     5341 tcgcaaagtt tagcagttat cggtagccat ttaccacttt tgagcaatat tccaacagtt
     5401 tatctacaaa ttgctccata tgtcctcact atcattgtat tagcagcttt ctttggacaa
     5461 gcagtagctc ctaaggcgga tggtattaat tatattaaaa cgaaataaat aaaaagaaac
     5521 tagtataagc tagtttcttt ttattgtttg tgaatttgtg aaaaaaatac tttgtgaaaa
     5581 tttcacataa attataatac aataacaata aaatataaaa aaatagaaaa ttgagtcatt
     5641 attctagtaa gagtttgtga ataatgttat aatgtgtttt gttattaatt atttataaag
     5701 gagaactctt aatatgagta aaatcgttgt cgtagggaca aaccacgctg gtacagcagc
     5761 aattaaaaca atgcttagta attatggtga agccaatgaa attgtaacat ttgatcaaaa
     5821 ttccaatatc tctttcttag gatgtggtat ggctctttgg attggtgaac agattgatgg
     5881 tccagaagga ttgttctact ctgataaaga gcagttggaa agtatgggag ctaaggttta
     5941 catgaactct ccagtactta atattgacta cgataaaaaa gaagttacag ctttagtaga
     6001 tggaaaagaa catgttgaaa gttatgaaaa acttatttta gcaactggtt cacaacctat
     6061 cattccacca attaaaggtg ttgaaattca agaaggatct cgtgaattta aagctactct
     6121 tgaaaacctt caatttgtga aactttatca aaattcagaa gaagttattg aaaagttagc
     6181 taaacctggt atcaatcgtg ttgctgttgt tggggctggg tatattggtg tagaattagc
     6241 agaagctttc caacgtattg gaaaagaagt aacacttgtt gatgttgctg atacttgtat
     6301 gggtggttac tatgatcgtg actttactga tatgatgagt aaaaaccttg aagaccatgg
     6361 tattcgtctt gctttcggac aagctgttca agcagttgaa ggtgatggta aagtagaacg
     6421 tcttgttact gataaagaaa catttgatgt tgatatggtg atcttagctg ttggattccg
     6481 tccaaataca gaactcggtg caggtaaatt agatacattc cgtaatggcg cttgggtagt
     6541 tgataagaag caagaaacaa gcgtgaaaga tgtttatgct ataggtgatt gtgcgacaat
     6601 ttgggataat tctcgtgatg atatcaatta tattgctctt gcttcaaacg ccgttcgtac
     6661 tggtattgta gcagctcaca atgcatgtgg tacagagctt gaaggtgctg gtgtccaagg
     6721 gtcaaatggt atctcaatct atggccttaa catggtgtca actggtttga ctttagaaaa
     6781 agcaaaacaa gctgggtaca atgcagttga aacaggcttt aatgaccttc aaaaaccaga
     6841 atttatcaaa cacaataatc atgaagtagc tattaagatt gtctatgata aagatagccg
     6901 agttatcctt ggttgccaaa tggtctcaca cgaagatgta tctatgggaa ttcatatgtt
     6961 ctcacttgct attcaagaaa aagttactat tgaaaagctt gctttgaccg atatcttctt
     7021 cttaccacat tttaataagc catataacta tattacaatg gctgcattag gcgctaaaga
     7081 ttaatttaaa attaaaaagg ttaagcgaaa tgcttagcct tttatttcat cctttaacaa
     7141 ggtcaaaaat gtaacggcaa agaaaaagct aagatattcc tatcctagct ttttttcaac
     7201 tatgttagtt ttttgatgct tcttggaatt ctgggttttt ccaagcttcg tcaataatat
     7261 cttttaattg ttcagctgag gcttgcattt tttgaagttc agcatcattt aatgggatat
     7321 taactggacg aacgatacca tgtgcaccta cgattgcagg ctgaccgata aagacatctt
     7381 taacatcacc gtattggcct tcttgatata cagataatgg aagaactgca ttttcatcat
     7441 caaggatagc tttagtaata cgagcaagtg cgacagcaat accgtaatat gtagcacctt
     7501 ttttgttgat gattgagtat gcagcgtcac gaactgaaat aaacaaatca acaagtcctt
     7561 gttcatcaat atcgcgattt tcttgtaacc attgttcaag ttggacacca gcaacgttag
     7621 catgtgacca aacggcaaat tctgagtcac cgtgttcacc catgatgtat gcgtgaactg
     7681 aacgagcatc aacaccaatt ttatctgcta aagcttgacg gaaacgtgca gagtcaagtg
     7741 aagtacctga accaataacg cgttccttag ggaaacctga gaatttccaa gttgaatatg
     7801 taagtacatc tacagggtta gccgctacta agaagatacc gttgaaacct gattcaacaa
     7861 cttgagttac gattgatttg ttaattgcta agtttttacc aacaaggtca agtcgagttt
     7921 cacctggttt ttgaggtgcg ccagcagtaa tgacaacaag gtcagcatct gcgcagtctg
     7981 cataagtagc tgcgtagatt tttttaggtg atgtaaatgc aagggcatgt gaaagatctt
     8041 cagcatcacc aacagcttta tcaaataaag ctgggatttc aatgatacct aactcttgcg
     8101 caataccttg gttaacaagt gcaaaagcgt aagaagaacc tacggcacca tcaccaacga
     8161 gaataacttt tttatgttgt ttagttgcag tcatttctaa acatctcctt aaatattttt
     8221 gggaagtttc ccatacacct atattctatc attttcaatg aaagaagtca ctaataaaat
     8281 gtgaaaaaat ttgcaaacaa attgaaatat catagaaaac tattgaaaac gctgagtgtc
     8341 ttgatatttt tgataccgat ttatcatcgc aattttacct aaattatggt acaatgtaag
     8401 agacttacag tgaagaaaag gggcattttt taatgcaaga taaaaattta gtagatgtta
     8461 atctaactag tgaaatgaaa acgagtttta tcgattacgc catgagtgtc attgttgctc
     8521 gtgcacttcc agatgttaga gatggtttaa aacctgttca tcgtcgtatt ttatatggta
     8581 tgaatgaact aggtgtgaca cctgataaac ctcataagaa atcagcacgt attactggtg
     8641 atgttatggg taaataccat ccacatggtg attcatctat ttacgaagca atggtgcgta
     8701 tggcacaatg gtggtcatat cgtcatatgc tcgttgatgg tcatggaaac tttggttcaa
     8761 tggatgggga tggtgctgcc gcacagcgtt atacagaagc acgtatgagt aaaattgctc
     8821 ttgagatgct tcgtgatatc aataaaaata cagttgattt tcaagataac tatgatggca
     8881 gtgaacgtga acctcttgtt ttaccagcgc gtttccctaa tttattagtc aatggtgcaa
     8941 ctggtattgc tgtaggtatg gcaacaaata ttccaccaca taatctaggt gagtctatag
     9001 atgctgtcaa attggttatg gataaccctg atgttactac acgggagtta atggaagtta
     9061 ttcctggtcc agattttcct actggtgcct tggtgatggg acgttcaggt attcaccgtg
     9121 cctatgaaac tgggaaggga tcaattgtct tacgttcacg gactgaaatt gaaacaactt
     9181 caaatgggaa agagcgtatt gttgtcacag agtttccata tggcgttaat aaaacaaaag
     9241 tacacgagca tattgtacgt ttagcacaag aaaagcgcat tgaaggtatt actgctgttc
     9301 gtgatgaatc aagtcgtgaa ggcgttcgtt ttgttattga agttcgtcgt gatgcttcag
     9361 caaatgtgat tttaaataat cttttcaaat taactagctt gcagacaaat tttagcttca
     9421 atatgcttgc tattgaaaaa ggtgttccaa aaattttgtc attacgacaa atcattgata
     9481 actatattga acaccaaaag gaagttattg ttcgtcgtac tcagtttgac aaagctaaag
     9541 cagaagcccg tgctcatatc ttagaaggtt tacttgtggc gcttgatcac ttggatgaag
     9601 ttatcacaat tattcgtaat agtgagacag acaccattgc tcaagcagag ttgatgtcac
     9661 gttttgaatt atcggagcgt cagagtcaag ctattttaga tatgcgtctt cgtcgtttaa
     9721 ctggattaga acgcgataaa atccaatcag aatacaatga cttactagct ttaattgctg
     9781 atttagcaga tatcttagct aagcctgaac gtgttgttac cattataaaa gaagagatgg
     9841 atgaggttaa gcgtaagtat gctgatgccc gtcgtacaga attaatgatt ggtgaagttt
     9901 tatcacttga agatgaagac cttattgagg aagaagatgt tcttattact ctctcaaaca
     9961 agggatatat caagcgtctc gctcaagatg agtttagagc acaaaaacgt ggaggacgcg
    10021 gtattcaagg gactggagtt aataacgatg attttgttcg tgaattggtt tcaacaagta
    10081 ctcacgatac tgtgcttttc tttacaaatt tagggcgtgt gtatcgtttg aaagcatatg
    10141 aaattccaga atatggacgt acagctaaag gcctccctat cgttaacctt ttaaaacttg
    10201 acgaaggtga gacaattcaa acaattatta acgctagaaa agaagatgtc gctaataaat
    10261 atttcttttt cacaactcag caaggtatag tgaaacgtac aagtgtttct gaatttagta
    10321 acattcgtca aaatggactt cgtgctatta atttgaaaga gaatgacgag ttgattaacg
    10381 tcctcttgat tgacgaaaat gaagatgtta ttatcgggac acgtacaggc tactctgttc
    10441 gcttcaaagt gaacgctgta cgtaatatgg gacgcacagc gactggggta cgaggcgtta
    10501 atcttcgtga aggtgataaa gttgtgggag cttctcgtat cgttaatgga caagaagtac
    10561 taatcatcac tgaaaaaggt tatggcaaga gaacagaagc ttcggaatat cctacaaaag
    10621 gccgtggtgg taaaggaata aaaaccgcta atattactgc taaaaatggg ccattagcac
    10681 gcttagtgac gattaatgga aatgaggaca tcatggtcat tacagataca ggtgttatta
    10741 ttcgaaccaa tgttgctaat atttctcaaa ctggtcgttc taccatgggt gttaaagtga
    10801 tgagacttga tcaagaagct aaaatcgtca ctgttgcgct agtggaacaa gaaattgaag
    10861 ataagtctaa tatagaggat acaaaagaat aggaagttat gcgtaataaa aaaaaatcac
    10921 atggtttctt taactttgtt agatggttat tggttgtatt gctaattatt gttgggttgg
    10981 ctttggtgtt caataaacca attcgcaatg ctttcatagc tcatcaatca aatcattatc
    11041 aaatttcacg agttagtaaa aaaacaattg aaaaaaataa gaaatctaaa acaagttatg
    11101 acttttcatc agtcaaatct attagtacag aatcgatttt atctgctcaa acgaaatcac
    11161 ataatttgcc tgtaatagga gggattgcta taccagatgt cgaaatcaat ctcccaattt
    11221 tcaaaggact gggcaataca gagttgagtt atggtgcagg aacgatgaaa gaaaaccaga
    11281 ttatgggagg cccaaataac tatgccttag ctagtcacca tgtctttggt ttgacaggtt
    11341 cttctaagat gctcttttca cctttagaac atgctaagaa aggtatgaaa gtttacctca
    11401 ctgataaaag caaagtatac acttacacta ttactgaaat ttcaaaggta acaccagaac
    11461 atgttgaggt tattgatgat acacctggca agagccaact cactttagtg acctgtacgg
    11521 atccagaagc cacagaacgt attattgtgc atgctgaatt agaaaaaaca ggtgaatttt
    11581 caacagcaga tgagtcgata ttaaaagcat tttcgaaaaa atataatcaa attaatctct
    11641 agaaggtagt tagaattatg aaattaaagg ctgttcatca tattgcgata attgtttctg
    11701 actatgaaaa gtcaaaagac ttctatgtta ataagctagg atttgaaatt attcgtgaaa
    11761 accatcgtcc ggagcgtcat gattataaat tagatttacg gtgcggtgat attgagcttg
    11821 aaatatttgg aaatcgtctt gatgatccag aatatgaaac tcctcctcag agaataggta
    11881 ggccgaattg gccacgtgaa gcatgtggtt tacgacacct tgccttttac gttccagatg
    11941 tggaagcgta caaggtagaa ctggagaatt taggaatttt tgtagagcct attcgttacg
    12001 atgactatac tggcaaaaag atgacctttt tctttgatcc agatggttta cctttagaat
    12061 tacatgaata ataaacaaag ttgcgtggat acgcaacttt gtttaattta ctgaataatt
    12121 ttatattcaa gaagtttttt aaagaaataa gtaagttaat catggtataa tgtaaggtat
    12181 gaaaagaatt gaaaaggaat tattttgaaa tgaggatact aaaatgagta ttaaaaagct
    12241 attgtttgca ggccttgctt ttattttatt taccctagcg agtccagcat atgctgcatc
    12301 agatgttcag aaagttattg atgaaactta tgttcaacct gattatgtct taggttattc
    12361 gctaaatcaa gaacagcgtg cacagacatt acaacttctg aattatgatg aatctagaga
    12421 cacgaaagtt aaaacgctaa atactagctc atatgctaaa ataatgaata tagctgatga
    12481 tgcaagcatc caattatatt catctgttaa aataaaaaaa ttagggtcta acgatacctt
    12541 agcagtaaac attgttacac ctgagaatat tactaaagtt acagaagata tgtatcgcaa
    12601 tgctgcagtt acattaggta ttgagcatgc aacgatatca gtggctgctc caataaaagt
    12661 aactggggag agtgctcttg caggtatcta ttattcatta gagaaaaatg gagcaagtgt
    12721 atcatcagaa aataaacaac tagcacaaga agagttaagt actttatctg gtattaatgc
    12781 tgagaataag ggtaaggaag gttatgacgc cgacaagctt aatgtagctt taacggatat
    12841 taagtcagcc gtagctaaag gaggatctga tctttcaaaa gatgatattc gaaaaattgt
    12901 tgaggaaaca ttgaaaaatt atcatcttga taacgctgta acggaaaatc aaataaattt
    12961 aattgttaat tttgcagtta atctttcaca aagtaatgtc atcaaaaata gtgatttcac
    13021 gaacaccttg aataatttga aagac
//
LOCUS       DATRSG010000060        12631 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRSG010000060 DATRSG010000000
VERSION     DATRSG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.060"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            22..3722
                     /locus_tag="VBD89_001633"
                     /pseudo
     CDS             22..3722
                     /locus_tag="VBD89_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            3932..4840
                     /locus_tag="VBD89_001634"
     CDS             3932..4840
                     /locus_tag="VBD89_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7136149.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            5394..6404
                     /gene="nrdF"
                     /locus_tag="VBD89_001635"
     CDS             5394..6404
                     /gene="nrdF"
                     /locus_tag="VBD89_001635"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7136150.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            6405..6818
                     /gene="nrdI"
                     /locus_tag="VBD89_001636"
     CDS             6405..6818
                     /gene="nrdI"
                     /locus_tag="VBD89_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7136151.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            6820..8985
                     /gene="nrdE"
                     /locus_tag="VBD89_001637"
     CDS             6820..8985
                     /gene="nrdE"
                     /locus_tag="VBD89_001637"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7136152.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            9048..12215
                     /locus_tag="VBD89_001638"
     CDS             9048..12215
                     /locus_tag="VBD89_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO7136153.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGDEHCNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQMEKNLKPVDYKTPIFN
                     KALPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            12228..12617
                     /locus_tag="VBD89_001639"
     CDS             12228..12617
                     /locus_tag="VBD89_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO7136154.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
BASE COUNT         4359 a         1962 c         2478 g         3832 t
ORIGIN      
        1 tttttggagg aaataggaaa agtggataaa catcactcaa aaaaggctat tttaaagtta
       61 acacttataa caactagtat tttattaatg catagcaatc aagtgaatgc agaggagcaa
      121 gaattaaaaa accaagagca atcacctgta attgctaatg ttgctcaaca gccatcgcca
      181 tcggtaacta ctaatattgt tgaaaaaaca tctgtaacag ctgcttctgc tagtaataca
      241 gtgaaagaaa tgggtgatac atctgtaaaa aatgacaaaa cagaagatga attattagaa
      301 gagttatcta aaaaccttga tacgtctaat ttgggggctg atcttgaaga agaatatccc
      361 tctaaaccag agacaaccaa caataaagaa agcaatgtag taacaaatgc ttcaactgca
      421 atagcacaga aagttccctc agcatatgaa gaggtgaagt cagaaagcaa gtcatcgctt
      481 gctgttcttg atacatctaa aataacaaaa ttacaagcca caacccaaag aggaaaggga
      541 aatgtagtag ctattattga tactggcttt gatattaacc atgatatttt tcgtttagat
      601 agcccaaaag atgataagca cagctttaaa actaaggcag aatttgagga attaaaagca
      661 aaacataata tcacttatgg gaaatgggtt aacgataaga ttgtttttgc acataactac
      721 gccaacaata cagaaacggt ggctgatatt gcagcagcta tgaaagatgg ttatgggtca
      781 gaagcaaaga atattttgca tggtacacac gttgctggta tttttgtagg taatagtaaa
      841 cgtccagcaa tcaatagtct tcttttagaa ggtgcagcgc caaatgctca agtcttatta
      901 atgcgtattc cagataaaat tgattcggac aaatttggag aagcatatgc taaagcaatc
      961 atagacgctg ttaatctagg agcaaaaacg attaatatga gcctgggaaa aacggctgat
     1021 tctttaattg ctctcaatga taaagttaaa ttagcactta aattagcttc tgagaagggc
     1081 gttgcagttg ttgtggctgc cggaaatgaa ggtgcatttg gtatggatta tagcaaacca
     1141 ttatcaacta atcctgacta cggtacggtt aatagtccag ctatttctga agatactttg
     1201 agtgttgcta gctatgaatc acttaaaact atcagtgagg tcgttgaaac aactattgaa
     1261 ggtaagttag ttaagttgcc gattgtgact tctaaacctt ttgacaaagg taaggcctac
     1321 gatgtggttt atgccaatta tggtgcaaaa aagattttga aggtaaggac tttaaaggta
     1381 agattgcatt aattgagcgt ggtggtggac ttgattttat gactaaaatc actcatgcta
     1441 caaatgcagg tgttgttggt atcgttattt ttaacgatca agaaaaacgt ggaaattttc
     1501 taattcctta ccgtgaatta cctgtggggg ttattagtaa agtagatggc gagcgtataa
     1561 aaaatacttc aagtcagtta acatttaacc agagttttga agtagttgat agccaaggtg
     1621 gcaatcgtat gctggaacaa tcaagttggg gcgtgacagc tgaaggagca atcaagcctg
     1681 atgtaacagc ttctggcttt gaaatttatt cttcaaccta taataatcaa tactaaacaa
     1741 tgtctggtac aagtatggct tcaccacatg ttgcaggatt aatgacaatg cttcaaagtc
     1801 atttggctga gaaatataaa gggatgaatt tagattctaa aaaattgcta gaattgtcta
     1861 aaaacatcct catgagctca gcaacagcat tatatagtga agaggataag gcgttttatt
     1921 caccacgtca gcaaggtgca ggtgtagttg atgctgaaaa agctatccaa gctcaatatt
     1981 atgttactgg aaacgatggc aaagttaaaa ttaatctcaa acgagaggga gataaatttg
     2041 atatcacagt tacaattcat aaacttgtag aaggtgtcaa agaattgtat tatcaagcta
     2101 atgtagcaac agaacaagta aataaaggta aatttgccct taaaccacaa gccttgctag
     2161 atactaattg gcagaaagta attcttcgtg ataaagaaac acaagttcga tttactattg
     2221 atgctagtca atttagtcag aaattaaaag aacagatggc aaatggttat ttcttagaag
     2281 gttttgtacg ttttaaagaa gccaaggata gtaatcagga gttaatgagt attccttttg
     2341 taggatttaa tggtgatttt gcgaacttac aagcacttga aacaccgatt tataagacgc
     2401 tttctaaagg tagtttctac tataaaccaa atgatacaac tcataaagac caattggagt
     2461 acaatgaatc agctcctttt gaaagcaaca actatactgc cttgttaaca caatcagcgt
     2521 cttggggcta tgttgattat gtcaaaaatg gtggggagtt agaattagca ccggagagtc
     2581 caaaaagaat tattttagga acttttgaga ataaggttga ggataaaaca attcatcttt
     2641 tggaaagaga tgcagcgaat aatccatatt ttgccatttc tccaaataaa gatggaaata
     2701 gggacgaaat cactccccag gcaactttct taagaaatgt taaggatatt tctgctcaag
     2761 ttctagatca aaatggaaat gttatttggc aaagtaaggt tttaccatct tatcgtaaaa
     2821 atttccataa taatccaaag caaagtgatg gtcattatcg tatggatgct cttcagtgga
     2881 gtggtttaga taaggatggc aaagttgtag cagatggttt ttatacttat cgcttacgtt
     2941 acacaccagt agcagaagga gcaaatagtc aggagtcaga ctttaaagtt caagtaagta
     3001 ctaagtcacc aaatcttcct tcacgagctc agtttgatga aactaatcga acattaagct
     3061 tagccatgcc taaggaaagt agttatgttc ctacatatcg tttacaatta gttttatctc
     3121 atgttgtaaa agatgaagaa tatggggatg agacttctta ccattatttc catatagatc
     3181 aagaaggtaa agtgacactt cctaaaacgg ttaagatagg agagagtgag gttgcggtag
     3241 accctaaggc cttgacactt gttgtggaag ataaagctgg taattttgca acggtaaaat
     3301 tgtctgacct cttgaataag gcagtagtat cagagaaaga aaacgctata gtaatttcta
     3361 acagtttcaa atattttgat aacttgaaga aagaacctat gtttatttct aaagaaggaa
     3421 aagtagtaaa caagaatcta gaagaaataa cattagttaa gcctcaaact acagttacta
     3481 ctcaatcatt gtctaaagaa ataactaaat caggaaatga gaaagtcctc acttctacaa
     3541 acaataatag tagcagagta gctaagatca tatcacctaa acataacggg gattctgtta
     3601 accatacctt acctagtaca tcagatagag caacgaatgg tctatttgtt ggcactttgg
     3661 cattgttatc tagtttactt ctttacttga aacccaaaaa gactaaaaat aatagtaaat
     3721 aaaactaagt aaactactcg gtaatatgat gaaaagttca gtttaacatt atgttaagct
     3781 ggacttttag ggtattgact cttatttttg attaatttta tattgattaa cggcatataa
     3841 tattataata ttataattac gattagtatg tgatactgtt cgctttgtaa gctttcataa
     3901 gatgaactag ccttaaggag tttggatagc aatgaaaacg ataaaagatt ggcaaataaa
     3961 tatttgctta gcgacctata atggtcaaaa ataccttcgc cagcaattag atagcataat
     4021 acaacaaggt tatactgact ggatttgttt aataagggac gatggatcta ctgatgatac
     4081 agtagctatt attaaagaat atgttaatag agattcacgt tttatattta ttaattctaa
     4141 cgatgataga aagttaggaa gtcaccgcag tttttacgag ttggttaact ataaaaaagc
     4201 agatttttat gttttctcag accaggatga tgtttggaaa gaaaatcgtt tagaacgtta
     4261 ccttgaagaa gcagagaaat ttaaccaaga attaccattg cttgtttatt cgaactggac
     4321 ttctgtagat gaaaaattaa cagtattgaa agaacataat ccagcaacag ttattcaaga
     4381 acaaattgcc tttaaccaaa taaatggtat ggttatcatg atgaatcatg agttagcaaa
     4441 gctttgggaa taccgtcaaa ttggcgccca tgattcttat gttggaactc tggcttacgc
     4501 cgttggaaat gtggcttata tttctgacag tacagtcctt tggcgtagac aagtaggggc
     4561 agagtcgcta aataattacg ggagacaata tggggtggct acgttttggc agatgataaa
     4621 tacttctttt gatcgtgctt cgcttatttt tgctcaagta tccgataaaa tgtccctgga
     4681 gcgtaaactc ttctttagtc gttttataga attaaaaaat gccaatctaa taagaaggat
     4741 ttatctttta tcaaaattaa aacttcgtcg gaaatcattg aaagaaacag ttgctatgac
     4801 tatcctactt ttaacaggtt acggtaaacc aaaagcataa tttttttggt tgaaggaaat
     4861 caagttgagt tgtagttaaa aatatagaaa atatttattt ttcaaaaagt tgttgacact
     4921 taacgaaatt agtgtataat aatattaaag aacaaggtag aaccttggct cgcattaatt
     4981 tcgggttttg gaaaaggagt agcaatcgct actccttttt tgttttacac aggaaaagta
     5041 tgaaggaaaa gccttttttc ttataaaaat taagcttgct taatttttta atttttatat
     5101 tctttacatt atgatgaaaa cacaagatat agtggtgata tatgttgaaa gacacaaaac
     5161 tttgtgttag tatactgttt aagcagatcc ttaataaagg taaccgacaa aaaagtttaa
     5221 cgtgacttaa caaaacagtg gttttgaagc aagagttagc ttcttaccag aataaaaaaa
     5281 catcaagaag aacattccaa tagttgtgtg attttaacaa aaatccagtc ctcttgggag
     5341 gcttttttgg tgcttaaaac tcgcttaatt ttaatattta ggagactagt ttaatgcaat
     5401 catactatga tcgttctcaa tcaccacttg actatgcctt gtcagaaaaa gcttttccta
     5461 tgcgttctgt taattggaat aagctaaacg atgacaaaga cttagaggta tggaacagag
     5521 taactcaaaa tttctggtta cctgaaaaga tcccagtttc taatgacctt aattcttgga
     5581 gaacattgga tgctgattgg cagcaattaa ttactcggac ttttactggt ctaaccttat
     5641 tagattctgt gcaggctact gttggtgata ttgctcagat taagcattct cagacagatc
     5701 atgaacaagt tatttatgct aactttgcct ttatggtagc tattcatgcg cgatcttatg
     5761 gaacgatttt ttcaacctta tgcactagcc aacaaattga ggaagcacat gaatgggtag
     5821 tggatactga aagtctacaa gctcgatcac gtatacttat tcctttttat actggtgacg
     5881 atcctttgaa atctaaggtt gctgcagcca tgatgccagg atttttgtta tatggtggct
     5941 tttaccttcc tttttacctt tcagccagag gaaaattacc aaatacgtct gatattattc
     6001 gtttaatttt acgagataag gtaattcata actattacag tggctataag tatcaacaga
     6061 aagttgctaa attatctgta gaaaagcaag ccgaaatgaa gacatttgtc tttgacctcc
     6121 tctatcagtt gattgattta gaaaaagcct atctttacga actatatgat ggttttgatt
     6181 tggctgaaga tgcaatcaga tttagcattt acaatgctgg taaattttta caaaatttag
     6241 gatatgattc tccatttaca gaagaagaga ctcgtatatc tcctgaggtt tttgcacaac
     6301 tttcggcgcg tgcagatgaa aatcatgatt tcttctcagg aaacggctct tcatatatta
     6361 tggggattac agaagaaaca ttggatgaag attgggagtt ttaaatggat agtacagtga
     6421 aggtggtcta tttttcatcg aaatctaaca atacccatcg ttttgttcaa aagttggctt
     6481 gttctaacca gcgtattcca tcagatggta gtagtatatt ggttactgag gattatattt
     6541 tgattgtgcc tacttacgct ggaggaggag atgataccaa aggcgcagtt ccaaaacaag
     6601 ttgtacagtt tttaaatgtt cgacaaaatc gggaacattg ccaaggggtt atttcttctg
     6661 gaaataccaa ttttggtgac acttatgcca ttgctggacc tattattgct cgaaaattaa
     6721 atgttccttt gcttcatcaa tttgaattac tgggaacaca agaagatgtt acaagggtaa
     6781 aggagttact ttgccaattc actagaaagg ataaataaga tgacacaatc agatgcatat
     6841 ctctcgttga acgcgaagac acgctttaga gatcgcacag gtaattatca ttttacttcg
     6901 gataaagagg ctgttgaaca atatatgata gaacatgttg aacctaatac gatggtgttc
     6961 acatcactaa ttgaaaagct agattatttg gtttctaata actactatga atcggacctt
     7021 ctaaaacaat ataaccttga gtttatttgc caaatttttg agcatgcata cgctaagaaa
     7081 tttgcttttc taaattttat gggggcttta aaattttata atgcttatgc tcttaaaaca
     7141 gaagacaatc gttattattt ggaacactat gaagatcgtg ttgtaatgaa tgctctgttt
     7201 ttagctgctg gtgatgaaaa agctgcctat gacctagtag atgacatgct agcaaatcgc
     7261 tttcaaccag caacacctac ctttttaaat gcagggaaaa aacgtcgtgg tgaatatatc
     7321 tcttgctatt tgttgcgcat tgaagacaat atggaatcta tttctcgtgc tattagtaca
     7381 agccttcagt tatcaaaacg aggtggtggg gtagcacttt gcttaaccaa cttacgggaa
     7441 tttggtgctc caataaaagg tattaaaaat caagctactg gtattgtacc tgtcatgaaa
     7501 ttactagaag attcgttttc ttacgccaat caattaggac aaagacaagg agctggtgca
     7561 gtttacttac acgctcacca tcctgaagta ttaacgtttt tagatactaa acgtgagaat
     7621 gccgatgaga aaattcgaat taaatctctt tccctaggac ttgttattcc tgatattact
     7681 tttgaattgg ctaaggcaaa taaagatatg gccttgtttt caccgtatga cattgagcga
     7741 gtatatggta aaccaatgtc tgatatctct atcacagaag aatatgaaac tttattagcc
     7801 aatgcggata ttcgtaaaac ctttattagt gcacgaaaac tatttcaaac gatagcagag
     7861 cttcattttg aatctggtta tccttatatc ttatttgaag atactgtaaa tgctaaaaac
     7921 cctcacaaaa aagagggacg aattgttatg tctaatttat gttctgagat tgcacaagtt
     7981 aatacagcta gtcaattttc agaagacctg actttcacta aggttggaca tgatgtttgt
     8041 tgtaatttag gttctattaa tattgcgcgt gctatggatc aagcggcgga ttttgaaaaa
     8101 ttaattgcta atagcattag agcccttgat cgtgtatctc gaacttctga tttagatagt
     8161 gctccatcta ttaaaaaagg taatgcagct aatcatgctg ttggtttagg tgctatgaat
     8221 ttgcatggat ttttggcgac taaccatatt tattacgatt ctcaagaggc tattgatttt
     8281 acagattgct tcttttacgc tatggcttat tatgccttta aagcttctaa ccatttagca
     8341 aaagaaaaag gaacctttga gggcttctcg gaatcaagtt atgctgatgg cagttatttc
     8401 tatcaatata ccgaacaaaa ctttgagcca aaaacacagc gtgttaaaaa tctactagct
     8461 gagtatggtc tgactctacc aagccaagag gattggagaa agcttgtgca aagtattaaa
     8521 gagataggat tagctaatgc gcatttatta gctgttgctc cgacagggtc aatcagttat
     8581 ctttcttctt gtactccgag ccttcaaccg gttgtatcac ctgtcgaagt acgcaaggaa
     8641 ggagcactgg ggagggttta tgtacctgct tataagattg atgcagataa ttatgtctac
     8701 tacaaaaaag gagcttatga agtgggatct gaggcgatta tcaatattgc agctgccgct
     8761 caaaaacaca ttgatcaagc tatttcgtta acgcttttca tgacagatca agcaactacg
     8821 cgagatttaa ataaagccta tattcaagca tttaaacaaa aatgtgcctc tatttattat
     8881 gtacgagtga gacaggacat cctagaaggt agcgagagtt atgatgatat gctggatgat
     8941 ttcacttcat cggacttaga agactgtcaa tcctgcatga tttaactaaa tatactcaat
     9001 cagaagatta agagaaatat attggctttt agaaaggctt tattgtaatg acaaaaaaac
     9061 atcttaaaac gcttgccttg gcacttacta cagtatcagt agtgacatac agccaggagg
     9121 tatatggatt agaaagagag gaatcggtca aacaagaaca aacccagtca gcttcagaag
     9181 atgattggtt cgaagaagat aatgagagga aaacaaatgt ttctaaagag aattctactg
     9241 ttgatgaaac agttagtgat ttattttctg atggaaatag taataactct agttctaaaa
     9301 ccgagtcagt ggtaagtgac cctaaacaag tccccaaagc aaaaccagag gttacacaag
     9361 aagcaagcaa ttctagtaat gatgctagca aagtagaagt accaaaacag gatacagctt
     9421 caaaaaagga aactctagaa acatcaactt gggaggcaaa agatttcgta actagagggg
     9481 atactttagt aggtttttca aaatctggaa ttaataagtt atctcaaaca tcacacttgg
     9541 ttttaccaag tcatgcagca gatggaactc aattgacaca agtagctagc tttgctttta
     9601 ctccagataa aaagacggcc attgcagaat atacaagtag gctaggagaa aatgggaaac
     9661 cgagtcgttt agatattgat cagaaggaaa ttattgatga gggagaaata tttaatgctt
     9721 accagttgac taagcttact attccaaatg gttataagtc tattggtcaa gatgcttttg
     9781 tggacaataa gaatattgct gaggttaacc ttcctgagag tctcgagact atttcagact
     9841 atgcttttgc tcacatgtct ttaaaacaag taaagttacc agataaccta aaggtcattg
     9901 gagaattagc tttttttgat aatcagattg gtggtaagct ttacttgcca cgtcacttga
     9961 taaaattagc agaacgcgct ttcaaatcta atcgtattca aacagttgaa tttttgggaa
    10021 gtaagcttaa ggttatagga gaagcaagtt ttcaagataa taatctgagg aatgttatgc
    10081 ttccggatgg acttgaaaaa atagaatcag aagcttttac aggaaatcca ggagatgaac
    10141 attgcaacaa tcaggttgta ttgcgcacaa ggacaggcca aaatccacat caacttgcga
    10201 ctgagaatac ttacgtcaat ccggacaaat cattgtggcg tgcaacacct gatatggatt
    10261 ataccaaatg gttagaggaa gattttacct atcaaaaaaa tagtgttaca ggtttttcaa
    10321 ataaaggctt acaaaaggta agacgtaata aaaacttaga aattccaaaa caacacaatg
    10381 gtattactat tactgaaatt ggtgataacg cttttcgcaa tgttgatttt caaagtaaaa
    10441 ctttacgtaa atatgatttg gaagaaataa agctcccctc aactattcgg aaaataggtg
    10501 cttttgcttt tcaatctaat aacttgaaat cctttgaagc aagtgaagat ttagaagaga
    10561 ttaaagaggg agcctttatg aataatcgta ttggaactct agacttgaaa gacaaactta
    10621 tcaaaatagg tgatgctgct ttccatatta atcatattta tgccattgtt cttccagaat
    10681 ctgtacaaga aataggacgt tcagcttttc gacaaaatgg tgcgcttcac cttatgttta
    10741 tcggaaataa ggttaaaaca attggtgaaa tggctttttt atccaataaa ctggaaagtg
    10801 taaatctctc tgagcaaaaa caattaaaga caattgaggt ccaagctttt tcggataatg
    10861 cccttagtga agtagtctta ccgccaaatt tacagactat tcgtgaagag gctttcaaaa
    10921 ggaatcattt gaaagaagtg aagggttcat ctacattatc tcagattact tttaatgctt
    10981 ttgatcaaaa tgatggggac aaacgctttg gtaagaaagt ggttgttagg acacataata
    11041 attctcatat gttagcagat ggtgagcgtt ttatcattga tccagataag ctatcttcta
    11101 caatggtaga ccttgaaaag gttttaaaaa taatcgaagg tttagattac tctacattac
    11161 gtcagactac tcaaactcag tttagagaaa tgactactgc aggtaaagcg ttgttatcaa
    11221 aatctaacct ccgacaagga gaaaaacaaa aattccttca agaagcacaa tttttccttg
    11281 gtcgcgttga tttggataaa gccatagcta aagctgagaa ggctttagtg accaagaagg
    11341 caacaaagaa tggtcatttg cttgagagga gtattaacaa agcggtatta gcttataata
    11401 atagtgctat taaaaaagct aatgttaagc gcttggaaaa agagttagac ttgctgacag
    11461 atttagtcga gggaaaagga ccattagcgc aagctacaat ggtacaagga gtttatttat
    11521 taaagacgcc tttaccattg ccagaatatt atatcggatt gaacgtttat tttgacaagt
    11581 ctggaaaatt gatttatgca cttgatatga gtgatactat tggcgaggga caaaaagatg
    11641 catatggtaa tcctatatta aatgttgacg aggataatga aggttatcat accttggcag
    11701 ttgccacttt agctgattat gaaggtcttt atattaaaga tattttaaat agttcccttg
    11761 ataagattaa agcaatacgc cagattcctt tggcaaaata tcatagatta ggaattttcc
    11821 aagctatccg aaatgcagcg gcagaagcag accgattgct tcctaagaca cctaaggggt
    11881 acctaaatga agtcccaaat tatcgtaaaa aacaaatgga gaaaaattta aaaccagttg
    11941 attataaaac gccgattttt aataaggctt tacctaatga aaaggtagac ggtgatagag
    12001 cggctaaagg tcataatata aatgcggaga ctaataattc tgtagctgta acaccaataa
    12061 ggtccgagca gcaattacat aagtcacagt ctgatgtaaa tttacctcaa acaagttcta
    12121 aaaataattt tatatacgag attctaggat acgttagttt atgtttgctt ttcctagtaa
    12181 ctgctgggaa aaaaggaaaa cgagcaagaa aataaggaga aattaatatg attacacaag
    12241 aaatgaaaga gattattaat agtcagttag caatggtcgc gacggttgat gctaaaggtc
    12301 aacccaatat tggcccaaaa cgttctatgc gtctctggga tgataaaacg tttatttata
    12361 atgaaaatac agatggtcaa actcgtatta acattgaaga taatgggaaa attgaaattg
    12421 cctttgttga tcgtgaacgt cttttaggtt acaggtttgt gggaactgct gaaattcaaa
    12481 cagaaggtac ttattacgaa gcagcgaaaa aatgggcaga aggacgcatg ggggttccga
    12541 aagcagttgg tattattcat gtcgagcgta tttttaattt acaatctggt gctaatgcag
    12601 gtaaagaaat cacttaatat aaaaaagcct c
//
LOCUS       DATRSG010000061        12492 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRSG010000061 DATRSG010000000
VERSION     DATRSG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.061"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..206)
                     /locus_tag="VBD89_001640"
     CDS             complement(<1..206)
                     /locus_tag="VBD89_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136155.1"
                     /translation="MIRHSFKSKMLLLVFILTIIVNYLSATGLLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVIL"
     gene            complement(330..542)
                     /locus_tag="VBD89_001641"
     CDS             complement(330..542)
                     /locus_tag="VBD89_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO7136156.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            complement(669..1955)
                     /locus_tag="VBD89_001642"
     CDS             complement(669..1955)
                     /locus_tag="VBD89_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7136157.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            complement(2085..3011)
                     /locus_tag="VBD89_001643"
     CDS             complement(2085..3011)
                     /locus_tag="VBD89_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7136158.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            3355..3630
                     /locus_tag="VBD89_001644"
     CDS             3355..3630
                     /locus_tag="VBD89_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO7136159.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
     gene            complement(3671..4102)
                     /locus_tag="VBD89_001645"
     CDS             complement(3671..4102)
                     /locus_tag="VBD89_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7136160.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(4099..4497)
                     /locus_tag="VBD89_001646"
     CDS             complement(4099..4497)
                     /locus_tag="VBD89_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO7136161.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            complement(4512..5285)
                     /locus_tag="VBD89_001647"
     CDS             complement(4512..5285)
                     /locus_tag="VBD89_001647"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO7136162.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            complement(5278..6213)
                     /locus_tag="VBD89_001648"
     CDS             complement(5278..6213)
                     /locus_tag="VBD89_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO7136163.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            complement(6441..6704)
                     /locus_tag="VBD89_001649"
     CDS             complement(6441..6704)
                     /locus_tag="VBD89_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO7136164.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            complement(6785..7243)
                     /locus_tag="VBD89_001650"
     CDS             complement(6785..7243)
                     /locus_tag="VBD89_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO7136165.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            complement(7206..9368)
                     /locus_tag="VBD89_001651"
     CDS             complement(7206..9368)
                     /locus_tag="VBD89_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO7136166.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            complement(9472..10515)
                     /locus_tag="VBD89_001652"
     CDS             complement(9472..10515)
                     /locus_tag="VBD89_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO7136167.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIILAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLSIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
     gene            10648..10776
                     /locus_tag="VBD89_001653"
     CDS             10648..10776
                     /locus_tag="VBD89_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136168.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            10782..11684
                     /locus_tag="VBD89_001654"
     CDS             10782..11684
                     /locus_tag="VBD89_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7136169.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            11684..>12492
                     /locus_tag="VBD89_001655"
     CDS             11684..>12492
                     /locus_tag="VBD89_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO7136170.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRV"
BASE COUNT         3921 a         2505 c         1899 g         4167 t
ORIGIN      
        1 cttaatatta ctaaaaaggt caataaatag atgactgacc aaattgaaaa ggctaatggt
       61 gctggtgtca agagcgtttg ataacgatca gacagactct tttgactgtt ccctgttaac
      121 aaacctgttg cggataaata attcactatt attgttaaga taaagactaa caataacatc
      181 ttacttttaa atgaatgtct aatcatagag gactcctttt acctccatta taattctttt
      241 agtttaaaaa ttctattttt ttaatcaaca tgtttactga atactcgctt gtctcgacaa
      301 aaaaagtggt ttttgaaacc actttttatt tatttacgaa aagcatctaa aatgacttta
      361 aattcttcat tagataaagt gataccttta cccatttttg tatgatctgg actccatgta
      421 cgcaaatcaa atttagctgg cgctccgttg aaactaacac gattcaactc ttttgtccaa
      481 cctttttcat tttcagatag gaccaataac ttctcaacaa tttcaaatgt aaattctgac
      541 attttatacc tccacttata ctattcgtta tttttatagc tttttgttct atttaaatga
      601 acttcttcta ttttaacaat ttttagtaat attgaccatg taaaatgaca aaatagttat
      661 aaaagtatct aagctcttat agtcttgcta gtaccatcat tttgataaag gttcactaat
      721 ccttctttac aagcacgaat catatcccct ttttttactg gatttggtaa tgttatggtt
      781 aataattcca tcggatttgg agcacggtct attttatttc cttcagcatc gcgcagacct
      841 tcaataaaac attcaaagtg acggaagcca ggaccataga attctacgcg atctccttcc
      901 ataataacat tacgctgacg aattgtagcc tccattttag cattgtcaaa tgaaaccact
      961 tccccaacaa atttgtattg aggaatttta cgacgagctc caaagagttg ttcgttttca
     1021 gttggtgtat ggtagtaaaa acctgttgct aattcgcgtt gtgcaacctt ccaaagttca
     1081 tcaatcaagt cttctttaat agcttcaaaa gcttctggac tttccatata ggcatctaca
     1141 gcagctttat agcaattagt tactgtagaa acataatgaa tggatttcat acgtccttct
     1201 atttttaaac tatctacgcc attttcaatc atatctggaa tatgctcaat catacacata
     1261 tccacagctg acattgagaa aggttctgga atctcgcctt ttaacgattg acgttcttgt
     1321 ccaaatggca tatcgtagag gtcatatttc caacgacatg actgagagca accgccacga
     1381 ttagcatcac gatggctcat atggtttgat agaacacatc gtcctgagta tgaaatacac
     1441 atcgcgccat gaacaaatgc ttctatctca acatctgtcc tcttcctgat ttcagccaac
     1501 tctgccatag taacctcacg tgctaatacg acacgagtaa gccccatctc tttccaaaat
     1561 tcaaaagttt catagttcgt ggaagaggct tgagttgaca aatgaatttc taaacctggt
     1621 gcttctgtag cacaaataac aataagagca ggatctgaaa caatgactgc atctagtccc
     1681 atatcacgta attcacgaaa ccacggacct gccccaagct cattaccttc atgagtaacc
     1741 atattagccg ctacataaac tttagcatct ctcgcatgag catagtttat tccctcttgt
     1801 aactcttcca tagaaaagtt accagctcta cttcgaagac catacgcttg acctccaaca
     1861 aatacagcat ctgctccata atcaatagca acttttaatt tttctagtgt tcctgcaggt
     1921 gataaaacct cagggcgttt ttttacatta gacatcttat atttccttct attttcaaga
     1981 taaaagaatt attttctatc ttaattaaca taccaatcga tatgcagttc ttagtgcaat
     2041 tggctacgct ttgagataaa aaactttcac gtagcaaata aatcttattt tactgttgat
     2101 gggtcaaaat cataaaatcc agtatctaaa ccacgtcctt tagggtgcag ttttctaatt
     2161 cgttcatcaa acaagaaagc ctgatcctga gtgaatgttc cattttcaat caaacgagct
     2221 gtttcgacaa aaatttcagc aatagcaaca aaattatctc ctggacaata aataccatct
     2281 aatttccagt gagtaaagtg atgttcaact agttcagtca ctttagtcat catattgata
     2341 tcattgttaa taaaaatatg agtcccatgc ttatcttcgt aaatagaata gtgactttct
     2401 ggatcacctg gttctgctaa aaaaagacca cgctcacgtg ttttttcatc agtgatatga
     2461 gtaaaattat aatagttttg caataacgga cgcttagagt gatgaattac agaagcccca
     2521 taaactaaaa tctcagcagg aaattcaagg ttctcagaca tcttaaaaag ttcttcagaa
     2581 ggtatttcac gtgctaaaac cgtttctact gctccatgtt gtccccaaaa attaacttga
     2641 cgacttgatg taacgaatac agaagtgtcg tagataagtt tgaagttata accatcacgc
     2701 ttgttgatat aaaatactcc tgcatcacca actaccaaat aatccacatt aatttctttc
     2761 atcaactcca aaaatggttt gatattatcc atcatttctt gatgcatgag tgcattacaa
     2821 gctactgtca attctttacc agcatcatgt accaatttag cgatttcacg caattcatcg
     2881 tctgaaaatg aatgtggtaa acgaagaccg taattttctt cccctacgta gatgcggtca
     2941 attcctattg ctagcaattg ctttacttgt tcaatagatt cagctgtcgc tgtcaaaatt
     3001 attttttcca taattagtat tataacaaac tttcagcatt tttaagcata tttcttgaca
     3061 agaataaata aaaaatattc ttttcgaaaa ttatatcact ttagaggtta agcactatca
     3121 gataagaaaa aaaacatagt attgaaatac tatacgaaat tatatctatt tttttccttt
     3181 ccaatttaaa aaagtaagga aagtagtaaa aaatactttt agtattctac tactaatatg
     3241 ggcaatgttt ttgttttttt gagaaaaaac ctttatttta actcatagta tcaaaaaact
     3301 agtgacaaac tctattattt gtgatatgat atgctagtat caaaggagaa agctatgtca
     3361 tcaccactta agcaagagca aactcacgaa agcaaaacag agcaagctca accaaaattt
     3421 caagtatttc aagaatttga tcacagtgct aagttgcgtg agctagtctt ttttgctcgt
     3481 atcgctctct tcagtataac cactgtgcta atctctttct tattactagc actaagttta
     3541 acacctgtgt ggagtttctt actagcgatg ctggcaagtc tagcagttac atctgcagtc
     3601 tcttcattca tttggtcact cagacaataa aaatctcata aaaaagaagc tacttatagt
     3661 agcttctttt ttatttttct ttatcttctg tgttttcttt ataatcaatg acaatatctt
     3721 ctacttttgt ttcaggagcc ttatcttctt ttttagcagt atcctcgtct gagaattttg
     3781 atttagcttg attgacaaaa tcattagcaa agtcaactac ttctccgctt ttttccttaa
     3841 cggctgagac gatatcctct gttgtcaatt cacctgattc aaatttacct ttataatctt
     3901 taaaagtatc aacagctaaa tttgaatatt cacttgcttt atctttagct atttgatgat
     3961 attcttctgg attttcttta tattctccat agaatttttc tgcatttttc cttagctctt
     4021 ttcctttttt tgttgttaaa aaataagcag ctgctgcacc tgatgcagca ctgataacta
     4081 aagttttaaa taatttactc attttttgtc tcctttttta cgaaaaattt ttgaagccgc
     4141 ttttccaata gctaaagctg accctgcctt agtaacactt gaggttgcac tacttgcctt
     4201 ttgtccaatg tgtcttgcct gtaggtttaa atcagataca gattctgata agtcagcaat
     4261 agctacaaaa agaggatcaa tagtagatac tttaccatta acatcatcaa ctaaaacatt
     4321 agctttggct aaaatttcat tagtttggta aagagtcacg ttaacatcgc ttgttaatac
     4381 tttaatagtt tgcttggttt cctcaatcgt ttctgatact tttttcagaa cgaaaagtat
     4441 tcctaaaact aaaacaacaa acgcaatggc aataattaat acagcaattt ctatcataat
     4501 gcctcctctg tttaataata tgaaatatgt tgttcacgac gatgataaat aatcatgaca
     4561 attccgataa taactaaaat aactgataac cattgtgaca cgcgcaaacc cgccaaataa
     4621 aggctatctg tcctcatccc ttcaataatg aaccgtccac atccatacca aactaaataa
     4681 aaacatgcca cttcaccttg ttttaaagtg cgcggtcgat gacgaataga catgatgatg
     4741 acaaaaccta ggaagttcca aagagactca tataaaaatg taggtactcg gtaagctcca
     4801 tcaatataca tttgattttt tataaagttt ggtaagtaat tcagattttt tactgcccta
     4861 ccgtatgctt cttgattaat aaaattcccc catctaccta tggcttgggc aatcatcact
     4921 cctggagcag caatatccag aaaatcaatg ggattaatca agcgacgata tgaaaaaatc
     4981 acaaggagaa tggctccggt aattaaccct ccgtaaatag caataccacc gttccaaatg
     5041 gcgataatct ctactgggtg cttagagtaa taagcccatt cgaagataac ataataaata
     5101 cgagctccaa caatggatag tggaaaagcc atgagaatga aatctagaat atcatccgac
     5161 ttaatgttct tacgaggggc ctctttcatt gctaagtaaa ctgctaacaa cataccagac
     5221 actatgcaaa tagcatacca gcgaatagaa aaggggccta attgtattgc tactggatta
     5281 atcatcattg acctcgttct gactaatcaa atgtgttaaa cgatcctcaa aagtctgggt
     5341 agcatcaaac cccatttgtt tagctctgtg attcatagcc gctgcttcaa taactactga
     5401 cacgtttcgg cctgttttaa caggaatacg tatccgagga accttaactc cagataattc
     5461 gatctcttca ttgccatttc cgagtctatc aaaaactttt ccagtttcaa agttttcaag
     5521 gtatatagct aattgcactt gcgatgaatc tttaaccgca ctagctccat ataggctcat
     5581 aatatcaatg attcccacac ctctaatttc aagtaaatga cgtaatatct cagcaggctc
     5641 tccccaaagc gtctcttcgt ctttagcata aacatcaaca cggtcatctg ctaccaaacg
     5701 atgtcctcgc ttaactagct ctagtcctgt ctcactctta ccaataccgg aatctccttg
     5761 aatcagaaca cccataccat agatatccat caagacacca tgaacacttg tcctctcagc
     5821 taagcaagaa tctaaatacc aagaaacttc acctgataaa cgacttgtag gggccttact
     5881 ttgaagaata gcaataccgg tatctttagc cgcctcatac atctcttctg gaatttctaa
     5941 atcacgtgct acaacaatag ctggtgtttc tttttggaac atctcacgta acacttgata
     6001 acgattatgt cctgtcatag ccattaaata tgaccactct ttcataccaa caagttgcaa
     6061 acgctctggt gcataataat caaaataccc tgtcatttct agaccaggac gtgaaatgtc
     6121 agctgttgta atccgcttag ataatagatg ttcatcacca tagatgacat ttaactttaa
     6181 gcggtcaact aacatttgaa cagtaactgc catacttact ccttcttata aaagtgaata
     6241 tccctattgc tataccctac aatttaaagt tgagcaaagg tactaaacac tttcattaat
     6301 ttacacccac attatatcat actttttact aaaagtaagc gctaacgcgt ttttctgaaa
     6361 aaaagaagat attttgaaaa aatggtctaa ataaagctaa gaaaaaagga gattcgtcta
     6421 gcaaatctcc tttatatgaa ttaccagaac caaccgtcat catcaacagg ttcagcatct
     6481 ttacgtcttc tgggaccttg tcttcttgtt tcaataatat cttctttata gggtaagaaa
     6541 acagctaata aaatatatag caaaagacca aatttggtaa aatacaaaat cagtgcaatc
     6601 aacacccgtg aaagagctaa atcccagtta tacttatcag ctagaccggc gacaacaccg
     6661 cagaccaact taccttttcg ttgtttgtag aagcttgatt tcatacctat ccttctttct
     6721 atctactcta tattttaatt ataactactt tgacagtaaa attaaatagg ccttacgact
     6781 gattttaaaa actctctatc aagcgaccat gacaataacc acaagcaaac ttgcgtaaat
     6841 taacacgtct cctacgtcgg tatacttgac cacaagattg gcaagagtaa tgatgataat
     6901 tcactttaac agccgtaact ctaggggtat atcgtaagcc atcgactttt tctagtaact
     6961 ctttaaaagc tttatcccta tgtcgatatc ctttaccttg tatatagaga tgataatggc
     7021 aaagctcatg tcttacaatc ttacgaaaaa tatcaatacc aaactcacga taaattttgg
     7081 gattaaagtc aaggtgtcta tcgtttggaa agaagcgtcc tccggttgtt cttaagcgat
     7141 tattccaatg tgcagtatgt aaaaactgac atccaaaatc ttctatagac accttcttaa
     7201 cataattagt caagtccgtc tggcttgata agactaagtc caattctgcc acgctccgca
     7261 tctacctcta caacccaaac agttacaata tcaccaacag agagatattt gcttggatgt
     7321 ggtaaaggag gcatttttct agttttttta tcgcgttttc gcttaataag gcgagactgg
     7381 tggattagac catcttcatg aacaccaata tcaacaaaag caccaaaatc aacgacgttt
     7441 cgaacagttc cttgcaattc ctgtccaact tttaaatcac tgacatctaa aacatcatgt
     7501 ctcaaaacag gcgcttcaaa atcatcacgc aaatcacgac ctggtttcaa aagatcttct
     7561 ataatgtctt ttaatgtttc ttgacctacc ccaatacttt ctgctgttgc aattaagtca
     7621 aggtgttgta acttctcttt tgcaagatca tctaattctt tgatagtcag ttggtcaagc
     7681 aactttttaa cagcttcata agactcaggg tgaacgcctg tattatccaa gaaattttta
     7741 gcattaggaa tacgtaagaa tccagcagct tgctcaaaag cttttgctcc taatctgggt
     7801 actttcttaa tttcagcacg tgacttaatt tgcccattct cttcacgata tttaacaata
     7861 ttttcagata ttgttttatt taaacctgaa acgtgagcta acaaggctgg acttgctgta
     7921 ttaacattaa cacctacttg gttaactaca gtttcaacaa caaaatcaag attttctgct
     7981 aatttttttt gactaacatc atgttgatat tgacccacac caatagattt tggatcaatt
     8041 ttgactagtt ctgctaacgg gtcttgcaga cgtcttgcaa ttgagatagc cgaacgtttt
     8101 tcaactgtta agtctggaaa ttcatgacgt gccaattctg aggctgaata aacagaggca
     8161 ccactttcgt taacaatcac ataggatacg tctggaaaat cttgtaaaac ttctgcaaca
     8221 aatgcttctg attcacgact cgctgttcca ttccctatag caataatttc tatattgaat
     8281 tctttgataa gttttgctaa ctctattttt gattgttcga tttttgcttg attagctgga
     8341 ggaactggat aaatcacttg tgttgtcatt aattttccag tttggtctac tactgctaat
     8401 tttgctcctg tacgaaaggc agggtcaaaa cctaacacca ttttcccctt aagcggtgag
     8461 actaacaata agttacgaag attctctgaa aacagtgaaa ttgcaccatc ttctgctcct
     8521 tcacttagtt ctgtacggat acggcgttcc atagctggaa caattttttt cttgacagtc
     8581 tgtacaataa ggtcatctat atattggcta gtttctttaa atcgaacagc aaaaaagcga
     8641 aacatttttt caaggttatg ctcaaaatta accttaagaa cacccatttt ctcaccacga
     8701 ttcaatgcta aaacttgata gccatggagt ttacttattt tttcagaaaa gtcatagtag
     8761 attttgaaga cctgtttttc atcaagtgac tcatctttaa caactgcagt gattgagctg
     8821 tagttccaga tctcattata tgtccatgaa cgtaatttat tatcctcaga aaaagcttcg
     8881 ataagaatat ctactgctcc agaaagggcc ttcgtagttg tctcaaaacc atctgtaaga
     8941 tagttttgag cttcttcctc aaggttatcc ttattttgta aaataagacg tgctaaagga
     9001 aaaagacctg cttcacgtgc aatcgtagct ttcgtacgac gtttttcttt ataaggtagg
     9061 tagagttcct caacatctgc tagtttagtt gcttcttcaa tagccttttt gagttcttga
     9121 gtcaactttc cttgttcttc aattttggct aagaccgtag ttttacgatc tgaaagagct
     9181 gtcatactct tatcaaggtc aataattgat ttgatttgaa cttcatccaa attacctgtc
     9241 atttctttac gataacgggc aataaaagga atagtatttc cttcatctgt taattctaaa
     9301 accttttcga tttgtgacgc tttaatacct aaatcaatag caatttgtgt aatatttaaa
     9361 ttttccataa cgcttttatt atagcatatt tttgacttac gaagagtttt aaaaagcgat
     9421 ttcaagatag cgtttttata atctaacaat aaaaaacaga ccaggtctgc tttaattctt
     9481 ttcaaaatat tttcgaacaa ttggtgctac ttcctcacca taaagtttga tggcatttag
     9541 gacgtctttg tgaggcatag agccaactgg tagatgtaac ataaaacggt ctaactcaag
     9601 cgcttcaaca agtcctataa ttttatgtgc aaccacttca ggacttccta caaaaatagc
     9661 accttctgga cctactgaac gtaaatactg ctctttagtc atttcagacc aatgagggcg
     9721 tcccttagca atattatcga cggtctgttt cgtagggaaa aaataacggt caatagcggt
     9781 ttgattatcc tcttcaatcc atccccaaga gtgagcagca acttttagtt gttctggttt
     9841 gtgtccatta cgactaccaa cttctttata aatatggacc aattgacgaa aggctttggg
     9901 attcccacca atagttgcat aaacaattgg caaaccttgt tctgcaatac gaattgtaga
     9961 atcaacattt cctcctgttg ccacccatat tgataactgt ctttgtaatg ctcttggata
    10021 aattggtcgc tcattaactg tttgtgtcaa atgacctttc caatcgagat ttgtcgctga
    10081 gttaattgct aacaacatat ccattttttc attaaataaa tcatcataat ccgctaaatc
    10141 gtatccaaac aatggaaaag actcaataaa ggaaccacgc cctgccataa tttctgctct
    10201 accatttgaa agtgcgtcaa tcgttgaaaa ttgctgatag acgcgaatag gatcattgga
    10261 agagagaatc gttactgcac tagataaacg gatattatta gttctaaccg ctcctgctgc
    10321 taggataatt tcgggtgcag agaccgcaaa atcttcacga tggtgctctc caataccata
    10381 tacatctaaa ccaacttgat cagctagttc aatctcagca actaattggc gaatcctctc
    10441 aggatgtgaa tagctttggt ttgtttcttc aaggattgtt gtctctccga atgttgtaat
    10501 acctaattca atcataatct tacctcatca atctactaat aacattttat cactatatag
    10561 tatcatttcc aatcgatttg tttttttatg ttaaaaataa gctaatatag tataataggt
    10621 tattatataa agaaagaggt gccattaatg gctattacgc acaaacgtca agatgactta
    10681 gaaagtatgt tcgctagttt tgctaaagta ccaaaaccta aaaaagttga ttctgattca
    10741 aaacctgaac agaaagatga ttctcagaaa gattaagttc tatggatatc tttaatcaat
    10801 taccggatag tgtccttcaa tggtttgcca ttttcatttc cattattatt gaggcacttc
    10861 cttttgtttt gcttggaact attttatcag gaattattga agtctttata acacctgata
    10921 tagtcaataa gttcttaccg aaaaataaat ttctcagagt cttatttgga accttcgttg
    10981 gctttgtctt tccttcttgc gaatgtggta tcattcctat tattaatcga ttccttgaaa
    11041 agaaagtgcc ttcatacacg gcagttccgt ttcttgctac tgctcctatt attaatccta
    11101 ttgttttatt tgcgacctac tcagcatttg gaaattctat acgcttcctg atattacgct
    11161 ttgtaggtgc taccattgta gccattgctt taggagtcat gcttgccttt ttagtagatg
    11221 ataatattct aaaagaagac gctaaaccta ctcattttca tgattattct gataagaagt
    11281 ggtatcaaaa aatctttcta gccttagctc atgccatcga tgaatttttt gacacaggac
    11341 gctatctagt atttggcact ttaattgctt cagctatgca aatttattta ccaactcgag
    11401 ttcttactac tatcggacac agccctatca cagccatctt agttatgatg ttgcttgctt
    11461 tcatcttgtc attgtgtagt gaagctgacg cttttatagg tgcttcttta ttatcaacct
    11521 ttggaatcgc gccagtgatg gctttccttt taattggacc catgattgat attaagaatt
    11581 taatgatgat ggtaaattct tttaaaacaa ggtttattgt ccaattcatt agtgtatctt
    11641 ctcttattat tattatctat tgtttatttg tgggggtaat ataatgattc gatttcttat
    11701 tcttgctggt tattttgaat taagcatgta tttaaagtta tcaggtaaac taaatcagta
    11761 tattaatact cattatacct atttggccta tatttcaatg gtcctatctt ttattttagc
    11821 aattgtacaa ctaattattt gggttaaaaa tatgaaaatg catagtcacc tacatggtaa
    11881 aatagctaaa tcaacaagcc caatgatttt agtatttccc gttttagtag gtttgttagt
    11941 acctacagtt agtttagact cgaccacagt ctctgccaag ggatataatt tccctctagc
    12001 agccggctct actggtactg tcagtcaaga tgggacacgc gttcaatatt tgaaaccaga
    12061 taccagcact tattttactt cttcagccta tgaaaaagaa atgcaaaaag agcttaaaaa
    12121 atataaaggc tctggtacat taaccatcac aactgaaaac tacatggaag ttatggaact
    12181 aatttatctc tatcccgagc aattcatgga tcgccaaata caatatactg ggtttgttta
    12241 taacgaaccc aaacacgaag gatatcaatt tattttccgc tttggaatca ttcattgtat
    12301 tgctgattca ggagtttatg gtctactaac aactggaaat caaaaaagtt atccggacaa
    12361 tacttgggta actgttagag gtacaattaa atccgagtat aatcaattgt tacaacaaaa
    12421 cttaccagtc ttacacattg aagagtctcg tcaagtttct aaacctaata atccttatgt
    12481 ttatcgtgtc tt
//
LOCUS       DATRSG010000062        12395 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRSG010000062 DATRSG010000000
VERSION     DATRSG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12395
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.062"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..483)
                     /locus_tag="VBD89_001656"
     CDS             complement(<1..483)
                     /locus_tag="VBD89_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7136171.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKEL
                     G"
     gene            complement(635..5959)
                     /locus_tag="VBD89_001657"
                     /pseudo
     CDS             complement(635..5959)
                     /locus_tag="VBD89_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            complement(6202..8193)
                     /gene="uvrB"
                     /locus_tag="VBD89_001658"
     CDS             complement(6202..8193)
                     /gene="uvrB"
                     /locus_tag="VBD89_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO7136172.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
     gene            complement(8254..9174)
                     /locus_tag="VBD89_001659"
     CDS             complement(8254..9174)
                     /locus_tag="VBD89_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7136173.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFTCFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGNIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
     gene            9357..11540
                     /locus_tag="VBD89_001660"
     CDS             9357..11540
                     /locus_tag="VBD89_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7136174.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            11540..12280
                     /locus_tag="VBD89_001661"
     CDS             11540..12280
                     /locus_tag="VBD89_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7136175.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
BASE COUNT         3855 a         2365 c         2005 g         4170 t
ORIGIN      
        1 tcctagttct tttttgttta ataaaatttt tcttataaaa ccatcttcaa gtcgcttact
       61 ataaaacatc tctgagatgt catcttcgtc aaatactctc aaaaggttaa gcatatcata
      121 tgctagttca atcttatcaa gttgtgaacg ttcaatccat ataacatcac cttcatctga
      181 cgattgtaat tcacctgaaa aatcatatgt tttataaagg aataccaaat aacggatgcc
      241 ttcatctgtg taccaatgct tcataccaac taatttggga ttacttattg ttaaacctgt
      301 ctcctcgcga acttctcgaa cgagagcatc atacaagctt tctccttctt caatatggcc
      361 gcctggtaga gcagctcctg accaagaata acgtttagga tcacggattt gcatcacaat
      421 acgtccttta ttatcttcaa taagacacat attagttaaa ataacttttt gactgcgtga
      481 cattatttcc tctagtatat aaatttagtg atagagttac aataccattt atttatcaat
      541 ttttctacaa aataatagat taagttaaaa ctcccttaat aatttatcca aaggaatttt
      601 agcttaataa attagaaaac taatttaatc aaaatcaata tttctttgaa gcttttttaa
      661 ggcctagtaa accaagtaca attactatga gtgcaattcc tttagccagt aaattattat
      721 tgtcatcact tgtccttggc aattcatgtt ttgccgctct agacattgga atcttagttg
      781 ttttcttcgc ttccatttga ctatgaacaa atgggggtgt ctttgtactc cctttcatgc
      841 ctacagctat cacttgtggt ttatccattt gatgtttaga tatttcttta aattcaccat
      901 gaactgctgg atcaacctta tgccctttta acgactctgt ttcttttttt agttttgtgt
      961 cgtcagagct tttatgatca tttttcaaac gtctgtcaga gttgatttct tccattgttt
     1021 ttacaatttg tatacttgtt tcgaaatggt tatatgcacc atttttaaat atcaaaccct
     1081 ttatagtatg atttcccact ttattatcga ctatttctgt caataaaata gagctatcct
     1141 tatccaagtc gaaaaggtcc gtaattcctt ttggatctgt taaaaggaga gtttgttttt
     1201 tcaaatgtgg ttttagtttt gaatctactt taaggtttga agttgataat aatgcttcaa
     1261 actctctaac cacagaacga ccaagggggt cagatatttt tgccgtaaag ctcacttttt
     1321 tgtccatgag ttggactggc ttaatcaatt ccgcagtctg attgtgggct agggataata
     1381 aattatcgat agaggtaaga tcttgcattt ggaaagaggt tttgcgtaaa cttgctgata
     1441 attcagggaa gttgccgatt gttataattt tttcagtgac tttacctgat ttatcaactg
     1501 ctgatacttt gatgacgtaa cgcccttcga aaccttttaa ttggtaagag tcaatacttt
     1561 gtccttctgt gtagggcttg ccattaacag ttatcattaa atttttaagg tgattatctt
     1621 ctgctttagc tataaccact ttgacttttc cggataataa atctgcttca ttaatagtta
     1681 acttcggtgt atccacatca ttaactacct tataatgttc agaaacggta ttacctgatg
     1741 tatctaccgc tgaaatcgta acaatatctc ctgctgctgc ttttatctta tgagtaaatg
     1801 ctcatctatt actgataaat tcaccacttg aatatttaac atcgacgcta tcaccattaa
     1861 tagaaagacc ccaacctttt acattatcag aaacgtgccc tttgatttca atttctccat
     1921 tttgagcata gatttgacca gtgataaagt tatctttgtc agcagcttct tctatgttag
     1981 tcttgtccag ttctaatttt ggaggttgta catcaacggt aatatgatag ccacgactaa
     2041 ataccaattt atcattctta ttataaacct tcaaattcat gatgttattc ccagacataa
     2101 atttgaccca tgtggtttta aatgtgtcag agtcatcacc atacatataa atagggttgg
     2161 atactgtacg atatgggtcg gaatgttttg gatctttagc gttctcatga aggttaacaa
     2221 ctcttatttc tgcacgatag ccatctttta ataagagaga aacactatgc tccaagcttt
     2281 cattttcaac atttcgaatt ttatttacag ttcgaattga atataccgtt tctcgagtta
     2341 ataataaacc atcctcgata tcagaaatat tttcttcaaa atcaaaatct ttttcttcgt
     2401 taaggtcatc atcgttatca tcccagatat cgtcatcatc tgaactggtt gcttgagctt
     2461 tcatattgcg atgagacagt gactgtggtt gtaatgtatt atcttcctca ctagagtaag
     2521 atttctctga tacttcttct gattctgata aagcaatttc cttgtttgaa ttcccagcat
     2581 agtctgcaac ttttatatcc agatcagatt ctcttaacgt taactcatca atatgatagt
     2641 gattttcggc aattttttta actgtgatag gcttttgtgt ctctttatta cgaacaatta
     2701 cttctttaac acgatgatta tcatgggtta aaatatcaaa gccgttatta acttttttaa
     2761 gagaaaaagt tggtctttca ttatctatta aaattggaag gtattggact tgataaggat
     2821 ctgtaggatt tgaacgtgca cgtaacttaa agtaatataa gcctggcttt gcagctttaa
     2881 aatcaccagt cttaggatca taaaactttc catcccaaac tagcgatgat tctgtcgttc
     2941 tcaaagcatc ataatatgag tcttcttcct catttcttga tttgtaatat ttctccacga
     3001 aatgtttcac tgttatacga cgaagtattg gtgaatcgtc ttttgcttct gatacaatag
     3061 agaggtcaaa atctgatgct gagcgtaata aagaaaatcg aggtgctaca cgcacaactt
     3121 tatcgggagc tacacgcaca ttagacatag taaataggga tggattgacc tttttactac
     3181 ctatttcttg tccgatttca taaaatttgt tagttccgtg tccaaccaaa gttgcttgca
     3241 tcaaggtcgt taatttattt ttagaacgac tatcccacat aggtgcgtca aaaattggtt
     3301 ctaaatccca tgttcctgag aagcccatgt aaggagctga gatatcttgt ccatctttat
     3361 ctaaagtttt gaaatacaaa tacccttcag caaaatcttg tataggtttt tttatctcaa
     3421 gatctatatc aagctctatg gctgattgcg gtgctagtga gataacttta cccatttgta
     3481 aagttgctcc ctctattact ttagcgtgaa ctgcctttaa aacgacattt tcatacccct
     3541 gattagtcac tttatcaaca attttagttg ttaaaatcgc tcctggttca atactaaagt
     3601 tacgacgttt ttgtcctgta ttgaccaatt tcaaggtaaa tcttttatgg ttcttaatct
     3661 cttttataga gaatgatcct gttttatcac caaaaacaag aacatcgtta gctaaagctt
     3721 tttctaagtt aaccatacct gcaccttgac gtcgtggtga aatttctaaa gctttattta
     3781 gagtatcttt caaaggttga cttgtattca tcaagacaat ctttgtcaaa tcaacgtttg
     3841 tcaaaccagc taacattgga agttttaatg ccattatttc agctacttta ctttttacta
     3901 atgcactaat ggcagctact tgaggggtag ccattgaagt tccagacatg tacccatagg
     3961 taccgtcgtt aaaagtagaa taaatatctt ctcctggagc aactacatct ggcttcaaat
     4021 gcaaatctgg tgtcactccc caactagaaa agcctgaaat tgtaggattt ggacggcgaa
     4081 caaaggtttc tttaggtaaa aattctaatt tagcagtttt gtttttcttg atgtagtcaa
     4141 tcaactgatc accatctttt tttgatagac taatagccca tttaccttct ggtatatcat
     4201 agtgataatc aaacatgggg tgagtatcaa aattcccaga actataataa gcaggttcat
     4261 ttatcataat aaccccttta gcaattccga taaaacgttt tagcttatct ttaacgccct
     4321 ctcctccacg ctcaacgacg ataacttttc cagtataact tttaaggtcc tcttctgaga
     4381 tatctttttt caactggcct ttaccaacat agactagatc aaatgtttta tttttgagca
     4441 tttttaatat tgtttgactt ttatgtccca gttctgcata tcctaattta agatttccaa
     4501 ctttcaaaac atctaaaaga agagcactat tttgaactga accaactcca ataaccttat
     4561 caacagctgc aacagacacc atagcagcag tatcttttgt tcccaaagag ttgtcaacga
     4621 cattatcgta ggtagtagat gaagccgaat taccatagtt tcccattgct gctactacga
     4681 taacaccctt gtcagtcacc ttcttaattg tctcatacca aagttcacct ggacgtcctg
     4741 ttcccaaata tccaaagctt aaactaataa cgtctgcccc tcttttagcg gcatcttcca
     4801 ttgcatggaa gactgcatca gagccggtcc actctgcaga tggatcttct ctatttgttg
     4861 aaattttgta cactaataac tgtgcattag gggcaactcc tttaaaacca tcctttgcat
     4921 ttcccgctaa gatacctgct aaatgcatac catgagggcg ctgaacgtcg tctttcaatt
     4981 ctaaattatc gcctcgttga taattgtatg caaaaggaac tttatttgta taaggagcct
     5041 taatatcttt aatacgacgc tttgccatca catctggatc attctcatcc aatcgcatgg
     5101 caggatgatg gatatctaca ccactatcaa tagtcgcaat aagtagccct ctaccgtcaa
     5161 ctttactatt ttgtttagct tttaaacgtg cagctaagac atgtgtccat tggttagcag
     5221 taaacatttg aggttttaat acttgaactg cttcaatatg ttttatctct ttaatcttac
     5281 caattttttc aatttctttt tcatctactg ctaatgaata gccattaaaa aggctactat
     5341 aagttaactt aatacgagtg cccggaattg ctttaagggc ttcatctact ttttccttat
     5401 ttacttgatc aaatgttaca ataacgcgct tatacacttt agaatcagat gggggagttg
     5461 gcttcgtcga agcattattc acgacatttt tcatatccac ttcattagcc ttattaggct
     5521 tattagtgtt tttgttttcc tcacttgaag gttgtgatga agtatttaat aacttttcac
     5581 ttgattgtac ttgcttttta gcctgtaaag aggtttcatc tttttgattt ttcgtttgtt
     5641 cttgaacgac ttgtttaaca ccagcaactt tatgtgcagg ttgttcactc tttagtatat
     5701 ttgaatctac agatgattta ttctcaagct tttgtgagtt actcgaggct attgttggag
     5761 gggttactgc attactttca gtacgtgact gtttaacttc ggaaggcttg atagttgtgt
     5821 ttttttctaa aagctgacta gtattcgcac tagtatgagg gactactgtg tctgcatcta
     5881 ccactacaga ggtcgctacc gcatttacag ctaaaaacgc aatcagtaca ccggttgttt
     5941 ttaaagaaat tcttttcata attccactca tttttaagtt agattatttt atttgttaag
     6001 caagcttaat ttaagcactt ttgtatagag ttgtcaatat ctatttgcta attaatgagt
     6061 attttttaag aatttttatc ttatttagat tagttctttt taaccaaact aaaaagctaa
     6121 ctctacctgc tagagtcagc ttatttaata atctataacc ttctgctatc tcctaaagag
     6181 agaaccagat gagagataag tttaatcgat tgcttttagt tctaagataa catctcggat
     6241 ttgtgcagct agctcaaagt ccaataattc agctgcatct tgcatttgct tttgaagagc
     6301 tttaatttta gcttgccttt cttttttaga taaactactg taatcgacaa ctttttcagg
     6361 cttatcgcta tcattacttt tagtaatagc aattaaatca cggatttctt tttttattgt
     6421 ttgtggcaca attccatgct tttcattata atccatttgt aaacgacgac ggcgagcggt
     6481 ctcatccata gctctttgca tagaatctgt tattttatca gcatacataa taacatgacc
     6541 attactgtta cgtgctgcac gtccgattgt ttggataaga ccacgctcat tacgaaggaa
     6601 accttcctta tcagcatcta agattgcaac aagggaaact tcaggaacat ctattccttc
     6661 acgtaagaga ttaataccaa ttaaaacatc aaacacacca agtcgtagat cacgaataat
     6721 ctctgtacgt tctaaggttt taatatcgct gtgcatatac ttaactttaa ctcccatttc
     6781 tttgagataa tctgttaagt cttctgccat acgctttgtc aatgttgtaa taaaggtacg
     6841 ctcacctttt tcagtcctca aattgatttc accaagcaag tcatccatct gccccatact
     6901 tggtctaact tcaacttctg gatcaagaag tcctgttggc cggataattt gctcaacaac
     6961 tgtatctgtt tgctccattt catagtctcc tggcgttgca gatacataga caatttgatg
     7021 gacatggctt tcgaattctt cacgacgaag tggacggtta tctaaggcag atggtaaacg
     7081 aaaaccgtaa ttaaccaaca tttctttacg cgaacggtct ccattataca tccctttgat
     7141 ttgccccatt gtcatgtgac tctcatcaat cataatcaaa aaatcttctg ggaagaaatc
     7201 taacaaagta aaaggtggct caccttcact tctaccatcc atgtgacgtg agtagttctc
     7261 aacaccattt gtatatccca tttcacgaag catttcaata tcatattctg tcctttgtcg
     7321 aatacgttgc gcttcaatca atttcccttc tttttcaaaa agttctactt gattttccat
     7381 ttcagcttgt atttttgaaa tagcttcttc catatgttca tcatttgtca taaagtgtgt
     7441 tgctgggaaa atagctaaat gttccacttc tcctagcaca cgccctgtta acgattctat
     7501 ttctcttata cgatcaattt catcaccaaa aaattcaatt cgaaaggcat gctcatcacg
     7561 actcgcagga aaaaattcta caacatcgcc acgaacgcga aattttccac gttgaaaatc
     7621 aatatcgtta cgctcaaatt gaatatcaac gagattgttc aataactgat cacgtgaaat
     7681 ttcttgacct ggcctaaggc taacaacact gtctgcatac tctttaggag aacctaaacc
     7741 gtaaatacaa gaaacagatg ctacaacaat aacgtcattt ctttctaata acgaagaagt
     7801 cgctgagtga cgtaatttgt caatttcgtc attgactgag ctatcttttt caatataagt
     7861 atccgaagaa ggcacataag cttctggttg ataataatca taatatgaaa caaagtactc
     7921 tacggcattg tcaggaaaaa attctttaaa ctctccataa agttgacctg ctagggtttt
     7981 attatgtgca atcaccaagg ttggcttgtt aacttgggcg ataacttggc tcattgtgta
     8041 ggttttacca gtaccagtag caccttttaa aatctgagct ttttctcctc cttcaatatt
     8101 gtcaaccaac gtttcaatag cttgaggttg atctcctgaa ggactatatt tggaaactaa
     8161 tttaaatcgg ttagtatctt ttctatctat catttcttta cctcgattac tttattttaa
     8221 catagaaaaa agaaaaagac tgagctgcaa cactcaatct gtttcttcag tcttaattag
     8281 ccatagaagg tatataattg cagctaagag aacaaaaata gaaatcaaac ttccttctat
     8341 tccgaatgca ccacctgata tccagtccgg tgcgttttct ttaacggtaa aatgcaaaag
     8401 agatgctcct gctttctgac cacttactgc aataccatag agattacctt ggctaaaatt
     8461 ccaagctcca tgtaaggctg caacgctcca aatattacca cttttaatca tatacaagga
     8521 cattaaaact cccgaacata taatacttac aatagaaaga aaagtgacat gggcatttac
     8581 caagtgcaaa atgccaaata aagtggatga gacccctatg gcaacagcta aatgaaaacg
     8641 atgattaatt agaggaagaa gccaccctcg tgttagcaat tcttccgtac cgctttgaat
     8701 aagccaaaaa ggaaggagag ttacaacata gaaaatcgtt gccttagaaa catcatagct
     8761 atgaaagtca agtcctccca atatataagc agtgatgaca gaaattgaaa atagaataaa
     8821 acctatactc cagcctttta taagtgacca cagagttctt ttaaactttg tagaaaaacc
     8881 caattctgaa aaaggtcttt tttctacgaa ttttacccag ataagcaaaa ggaatgctga
     8941 aaaactaaag gttcctaatt ctccatagat attattaaat aaattaataa actcaaaaaa
     9001 agtataggaa gaaaacttat agagtacaga agacactaga acaattccca caccacctag
     9061 agttagaccg acgaaaaaaa ataggtatac cataaaacaa gtaaataaca ataatagcca
     9121 tgacggccag tttttataga cagcgaactg actatttaaa atccttttat tcataataat
     9181 cctaaatcct ttttgtgtga tgacactaga acaattatat cataatacta actaaagata
     9241 tatataaaat taacataaca tacatgttac gttttctaac atagaaatga cattttgttg
     9301 ctctatctta tttattttga tataataaaa cacatattta taaaaaggag atttacatga
     9361 agcacaagtt aaaagctttt acgcttgctt tactctcaat attctttgtg tttggtggaa
     9421 aggtcagtgc agagactgtg aatattgttt ctgatacagc atacgctcca ttcgaattta
     9481 aagattctga tcaaacttat aaaggaatcg atgttgacat cgttaacgaa gtcgctaagc
     9541 gtgctggctg gaatgttaac atgacgtatc caggttttga tgccgcagtt aacgctgttc
     9601 aatctggaca ggcagatgcg ctaatggccg gaactactgt tactgaagca cgtaaaaaag
     9661 tctttaattt ctcagatact tattacgata cttccgttat tctttatact aaaaataata
     9721 ataaagtcac aaactacaaa caactaaaag gaaaagtagt cggtgtaaaa aatggaacag
     9781 ctgctcaaag cttcttagaa gaaaataaat ctaaatacgg ctataaagtt aaaacatttg
     9841 atacaagcga cctaatgaat aacagccttg attctggttc tatttacgcc gctatggacg
     9901 atcaaccagt tgtgcaattt gcgataaatc aaggaaaagc ttacgccatt aacatggaag
     9961 gcgaagcagt tggtagcttt gcatttgctg tcaaaaaagg tagtggacac gataatctaa
    10021 ttaaagaatt taacacagct tttgcacaaa tgaaatcaga tggcacttat aatgacatca
    10081 tggataaatg gcttggaaaa gacgctacaa aaacaagcgg caaagcaaca ggtaatgcca
    10141 atgaaaaagc aactcctgta aagccaagtt ataaaattgt ttctgattct tcattcgcac
    10201 cattcgaata tcaaaacggt aaagggaaat atactggttt tgatatggaa ttaatcacga
    10261 aaattgctaa acagcaaggt tttaaacttg atatctcaaa tccaggtttt gatgccgctt
    10321 taaatgctgt ccaatctggg caagctgacg gtgttattgc aggagccaca atcacagaag
    10381 cacgccaaaa aatctttgat ttttctgatc cttattacac atctagcgtt atcttagcgg
    10441 ttaaaaaagg aagcaatgtc aaatcatacc aagatttaaa aggaaaaaca gttggtgcta
    10501 aaaatggtac tgcctcatat acttggttat cagaccacgc agataagtac aactatcatg
    10561 ttaaagcatt tgatgaagca tctacaatgt atgatagtat gaactcaggt tcaattgatg
    10621 ctctaatgga tgacgaagcc gttcttgctt acgctattaa tcaaggtcgt aaatttgaaa
    10681 cacctatcaa aggtgaaaaa tcaggcgata tcggatttgc agtgaaaaaa ggggcaaatc
    10741 cagaattaat taaaatgttt aacaacggtc ttgcttcact caaaaaatcg ggtgagtacg
    10801 ataaacttgt taaaaaatac ctttccacag ccagcacttc ttcaaacgat aaagctgcta
    10861 aacctgtaga tgaatcaact attttagggt taatttctaa taactacaaa caattgctat
    10921 ctggtattgg aactacttta agtttaactc ttatctcgtt tgcgattgct atggttattg
    10981 gtattatctt tggtatgatg agcgtatcac caagtaatac tctccgcaca atttcaatga
    11041 tttttgttga tattgtccgt ggtattccac tcatgattgt ggccgctttt attttctggg
    11101 gtattcctaa tttaatcgaa agcatcacag gtcaccaaag tccaattaat gacttcgttg
    11161 ctgctactat cgctctttct ttaaatggtg gtgcgtacat tgctgaaatt gtacgtggtg
    11221 gtattgaagc tgttccttct ggtcaaatgg aagcaagtcg cagcttaggt atttcttacg
    11281 gcaaaactat gcaaaaggtt atcttacctc aagcagtacg ccttatgtta ccaaacttta
    11341 tcaaccaatt tgtcatctca ttaaaggata caacaattgt atcagcaatc ggacttgtgg
    11401 aactcttcca aactggtaaa atcataattg cacgtaacta tcaatcattc cgtatgtatg
    11461 ctatccttgc aattatgtat cttgtaatca ttacccttct cacccgtcta gcaaaacgtt
    11521 tagaaaagag gcttaaataa tggcagaatt aaaaattgat gtccaagatt tacacaagtc
    11581 gtatgggcaa aatgaagttt taaaaggtat tgatgctaaa ttttatgaag gtgatgttgt
    11641 ttgtattatt ggaccttctg gttcaggaaa atcaactttc cttcgcacat tgaacttact
    11701 tgaatcaatc acaagtggaa aagttgttgt ggacggcttt gagttatcaa atccaaaaac
    11761 agacatagac aaagcacgtg aaaatatcgg aatggtattt caacacttca atctttttcc
    11821 acatatgtca gttcttgaaa acatcacttt tgctcctata gaacttggta aagaatctaa
    11881 agaagcagct gaaaagcatg ggatggaatt acttgaaaaa gttggtttag ccgataaagc
    11941 taatgccaaa ccagatagtt tatctggagg acaaaaacaa cgtgtagcta tcgcaagaag
    12001 cttagcgatg aatccagata tcttactttt tgacgaacca acttctgcac ttgatccaga
    12061 aatggttgga gatgttctca atgttatgaa agatttagct gaacaaggta tgactatgtt
    12121 aattgtgaca cacgaaatgg gatttgctcg tcaagttgcc aatcgtgtta tctttacaga
    12181 tggtggtcgt ttcctcgaag atgggacacc tgagcaaatc tttgatacac ctcaacaccc
    12241 acgtctccaa gacttcctta ataaagtttt aaacgtttaa taaaaaaaga tgacttcgtg
    12301 tcatcttttt ttatatcctg atcacaaaca aattataata ggataagttt tcttctaaaa
    12361 ttagcaaaac atcagctaga atgtttgaat aatgt
//
LOCUS       DATRSG010000063        12385 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRSG010000063 DATRSG010000000
VERSION     DATRSG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12385
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.063"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..1830
                     /locus_tag="VBD89_001662"
     CDS             <1..1830
                     /locus_tag="VBD89_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO7136176.1"
                     /translation="FTNSLISSIPSLNLSELHLTQEEKAIQFTSSDSEIIKKVSNERT
                     LYLNTNLLNRLFEARKNRDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTV
                     NGKQTRTLDPSQNNNIIINSSYQGELSNSSITKPSYTITIGNQKQIVQNDDGEIQRSY
                     LEAEATYQRTLQEVIDAYNTTHDLVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVK
                     NGITKYLMDENTADAQQKVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETL
                     QTINMKVQNITQDQSKVNDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQ
                     VNQIFTSFNKDVQDAKNSGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNG
                     VPNDILLNFLSRPVAESASAVRATENTYRPFTWILLLEVVSLFIAYIFATQNLIKKLT
                     DRYNVNRWLQTDFLNIIVISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFL
                     LIHSQYFFIKNFKAVGMGLALFMIISFVYLSNAVGTVATVSGFPKLLKAINPLSILEN
                     QLSSYFDNVTTGFIFLILVLLVDVAFIIMNIFITLNFEAKVKE"
     gene            1839..2312
                     /gene="essA"
                     /locus_tag="VBD89_001663"
     CDS             1839..2312
                     /gene="essA"
                     /locus_tag="VBD89_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO7136177.1"
                     /translation="MKLKRFLACLLILSCMSQTSVFSDGLKNNDIHFDTKRLESDSQK
                     NSGFQTELTKDLFKKEDLKKLDKNRQSKVSLQKKQSKQLFQYSNKGQSDRKKRIKQLF
                     SEESNQFKFVTRTEEATVSADIGHMIMYLVLISLVLILASIMTFKIKDKKDEYTY"
     gene            2296..2538
                     /locus_tag="VBD89_001664"
     CDS             2296..2538
                     /locus_tag="VBD89_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO7136178.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            2538..3812
                     /gene="essB"
                     /locus_tag="VBD89_001665"
     CDS             2538..3812
                     /gene="essB"
                     /locus_tag="VBD89_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO7136179.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAINADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYYAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGCGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
     gene            3819..8247
                     /gene="essC"
                     /locus_tag="VBD89_001666"
                     /pseudo
     CDS             3819..8247
                     /gene="essC"
                     /locus_tag="VBD89_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
     gene            8252..8968
                     /locus_tag="VBD89_001667"
     CDS             8252..8968
                     /locus_tag="VBD89_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136180.1"
                     /translation="MKKQRLLLLFGGLLIMIMMTACKDSKIPENRTKKEYQAEQNFKS
                     YFKYISDKNNYLDNIKVYYFSISISKDVQDKVSETTTCSYRLEKQKNQEFIGNFEHEV
                     SESSQYSTEVKNQIQYPIQYKDNSIRFTEKTPSERYDEFVFSSFDSSLLKKYKIYDYL
                     LKHPETELKGVSYKIPINSEIVAPFIKQFNIKNPKKSSISVTKTESKEYYYTISIDTD
                     SEIYSIFEGITLEDVKEFSK"
     gene            8965..9495
                     /locus_tag="VBD89_001668"
     CDS             8965..9495
                     /locus_tag="VBD89_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136181.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQINGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            9587..9810
                     /locus_tag="VBD89_001669"
                     /pseudo
     CDS             9587..9810
                     /locus_tag="VBD89_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10142..10618
                     /locus_tag="VBD89_001670"
     CDS             10142..10618
                     /locus_tag="VBD89_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136182.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            10874..11211
                     /locus_tag="VBD89_001671"
                     /pseudo
     CDS             10874..11211
                     /locus_tag="VBD89_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11605..11985
                     /locus_tag="VBD89_001672"
     CDS             11605..11985
                     /locus_tag="VBD89_001672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136183.1"
                     /translation="MVFVSDGSPVDANTLYFGKGTPIFMIMNVTIFIISTLIYSYYYL
                     PKNQGKVWKFNQWNNEKSREQQYISNFIVTFGLILLVTPFLKYGIYNSFGVLISLLFT
                     ATLPSLIVDSLYAAFYVHKHPEYE"
BASE COUNT         4367 a         1984 c         2190 g         3844 t
ORIGIN      
        1 ttcacaaatt ctctcatttc aagcattccc tcactaaacc tttcggagtt gcacttaacc
       61 caagaagaaa aagccattca gtttacatct tctgattcgg aaatcattaa aaaagtaagc
      121 aatgaaagaa ctctgtattt aaatactaat ttattgaatc gtctttttga agctaggaaa
      181 aatagagatg aagccaaaaa taaagttaat caattaaaat tgccatctag ttctactcga
      241 actggtgagc agatagtttc tgttgaatca aataatccag attacagggt agatacctgg
      301 acggttaatg gtaagcaaac aagaaccttg gaccccagcc aaaacaataa tattattatc
      361 aatagcagtt atcaagggga attatctaac tctagtatta caaaaccaag ctatacaatc
      421 actatcggga atcagaaaca aattgtacaa aatgatgacg gtgaaattca acggtcttac
      481 ttggaggcag aagcaacgta tcaacgaact ctacaagaag taattgatgc atataacacc
      541 actcacgact tagtagctaa atactacatt atttctgatg gcgaagaacc gcaaaattta
      601 tttgatcaat ttttaaatca aagtgttaac gatacgatgg ttgatctggt caaaaatggt
      661 ataactaagt atttgatgga tgaaaacact gctgatgcgc aacagaaagt taaagatgtc
      721 atggaggaag ttgaaaatag ccaagatgaa ttggcggatc aaatggcaaa agtaactgag
      781 acaaatgtac gactaacaga tacaatcaaa aaacagcttg agactcttca aacaattaac
      841 atgaaggtcc aaaacatcac acaggatcaa tctaaagtaa acgactcaca gaaaacaact
      901 gatcaacaat tatctgattt gaagaaccag ctagatgggc taatgacctc tgcaactggc
      961 gtaaaagata tgtccaaatc taatagtcaa gaggcggacc aagttaatca aatattcaca
     1021 tcattcaata aagatgttca agatgctaaa aattcgggca acaaactttc gacggatgca
     1081 acagatttaa tggctaactt ccaaaaagaa ttggccaata acggtgattt cgtagcttct
     1141 ttctctaaag tttttaatac tgcttataaa aatggagtgc caaatgatat tctccttaat
     1201 ttcttatcac gacctgtcgc tgaatcagcg tctgcagtta gggcaactga aaatacttat
     1261 cgcccattca cttggatact attattagaa gttgttagcc tatttatagc atatatcttt
     1321 gcgactcaaa accttattaa gaaattgaca gataggtata atgtcaatcg ctggcttcaa
     1381 acagactttt taaatatcat tgtcatttca ggcctctctc ttgtgattgg tttagcactg
     1441 ggagttatct caagcagaag tcttcatgtc atgcctgaat atgtaccatc ttggttccta
     1501 gttatgactc tgtttagttt tttattaatt catagtcagt atttctttat taaaaatttt
     1561 aaagcagttg gtatgggttt agctttgttt atgattatta gttttgtgta tctatcaaat
     1621 gccgtaggca cagtagcgac tgttagtgga tttccaaaat tactaaaagc tattaatcca
     1681 ctatctatcc ttgaaaatca attatcatct tattttgata atgtaacaac tggatttatt
     1741 ttccttatct tagtacttct tgtggatgtt gcttttatta tcatgaatat ttttatcacc
     1801 ttaaattttg aagctaaagt aaaagagtga ggttgttgat gaagttgaaa cgatttttag
     1861 cctgtctact aatattatcc tgtatgagtc aaacatcagt attttctgat gggttaaaga
     1921 ataatgatat tcattttgat acaaaacgtt tagaatcaga ttcacaaaaa aattctggtt
     1981 ttcaaactga attaactaaa gatttattta aaaaagaaga ccttaaaaaa ttggataaaa
     2041 accgtcaatc taaagtctca ctccagaaaa agcagtctaa gcaactgttt caatattcaa
     2101 ataaaggaca atctgatagg aaaaaacgta tcaaacaatt attttctgaa gaaagtaatc
     2161 aatttaaatt tgttacaaga acagaagaag cgactgtttc ggctgatatt ggtcatatga
     2221 ttatgtacct ggttctcatt agtttggtac taatattagc ttctataatg acttttaaaa
     2281 taaaggacaa aaaggatgaa tacacatatt aacattactc tagatatggg ggaaacctca
     2341 aaagatttac gtatcccttt gaaaattagc gttaagcaat tagtaatgga attagatagt
     2401 atttttagcc gtactgaaag gcgtactaaa tatcaactaa gagttataaa taaaggtatt
     2461 ttattgggag agaacgatat attagcgcac catccagtaa caactggtga tcgtataagg
     2521 atagaggagt tttattaatg gaaacagatt cgtttgtatt tgaagaacaa gactttcaat
     2581 ttgaaaaagg accagatttt tggcagttat ctcttaagcg ttcaaaagta ggaactcaaa
     2641 atctgcaaca actgcaattg ttagaaattc accattcttt gttgatgcct atgacaactg
     2701 caattaatgc agataccatt caatttcaat tccagacaga agcgcacgct ctatcttttg
     2761 aaagctttaa aaaagaaact ttatctgaaa aattacgatt agccttgaat gtcttggatt
     2821 tagataaggc tttgtctttg ccagtaaatt tcattttgca tccctctaat ctatttttaa
     2881 caaaaaatgc aactgctaaa atagcttatc gtagtcttcc tgggattatg acacctgaga
     2941 agtttggtcc agaggagttt ttatatcaat tcaaatgttt tgtctttgca ttactgacgc
     3001 aacatgacta tatagaattg tataatggtg ctatttctgt tattgaagta tcagatttcc
     3061 taaaaagcat ttattatgca gaaactattc aggctgttag agacatcatt actactgatt
     3121 atgagcagca agtagaagta gaaactcata cgttagcaaa agtatcaaag gcaaaatata
     3181 agctttataa atacataagt gtctggctag gagctttatc tacaatactc ttgattccgc
     3241 tagtatatct ggtttttatt cacaatccat tcaaagaaaa aatgttggca gcggatactt
     3301 catttattaa agttgattat aatcaagtga ttaatcgact agaacatgta aaagtaagta
     3361 agttacctta tacacagaag tacgagttgg cctattccta tattaatgga atgtcatttt
     3421 ctgaagaaca gcgtgaagtt attttaaaca atgttacgct caagactgat gagctctatc
     3481 ttgattattg gattaatatt ggctgtggtt tagatgatga tgctattgat gctgccaaac
     3541 gtttagacga ttctgacctt gttatttatg ctattgttca gaaaatggat caggttagaa
     3601 aagacaatag tttatctggt aaagatcgtg aacaaaaact ttctgagcta caaacagact
     3661 atgataaata ttggaaagac cgtaagacag ccttaacaga tgaggaatcc aaatcgaaaa
     3721 atagcaataa tcattcgaca aattccaaca aagagtcatc tgaatcatca agtactacag
     3781 caagtacatc ttctaaaact aaaagtaggt aggtggatat gttagttatt tgttattatc
     3841 aatcactccg ctacgaattt aatcttgaag aggagaaatc ctttctaata tcatcaaacg
     3901 gcaagtcgcc aataccagta tcagatttag aaaatgacat cacgctaaaa aatatgcaag
     3961 gtcaattggt atatattatt gaccaaaaag aaaaagagtt aactaatggt gtagagattt
     4021 caggaattgt cttctattta gcgaataatc aaaaagaaat ctatactccc ttagattacg
     4081 aagatatatt aatcggtgat aaagaagggt atcatgtccg ttttaaagaa ggagccccta
     4141 atttattact gaaaaaaata gaaagcaatt ggcagcttaa tttatttgaa ggtgatattt
     4201 atctgaataa tcatcttcaa aaagtagtgc agcagttacc tttaagttta ggtgatgaga
     4261 ttagcttcca aggaacaatt gttaagcttt tcccagatga aattcaaatc tggggggggg
     4321 actgattatg aaacatcatt aactaagaaa gttatgtcag cttatcaatt ttacgaaggc
     4381 tatcctgatt ttcaccgctc acctcgtatt atttatcgtt ccagtgaaga taaaattaca
     4441 gttaatgcgc ctggtaataa acctaataag tctaaagatg aactgttaaa attaattgtt
     4501 cctcctctag tgatgattgg agtcagtatt cttatttcta tttttcgtcc tcgtggtatc
     4561 tatatcattg caacaatgtc aatggctcta gtgacgatga ttttctctat cactggttac
     4621 tttaaaaatc gtaaacagta taagcaggac ttacaagaac gcattgattc ctatcatgac
     4681 tatttatctg ataagtcaat tgaacttcag aagttagcta aagagcaaaa gcgtgggcaa
     4741 cactatcatt acccaacgat tgaaggttta caggaaatgg ctgatactta tcaccatcgt
     4801 atttatgaaa agacaccact tcattttgac ttcttgtatt atcgcctagg tttgggagaa
     4861 gtaccaacga gctataatat tcattattct caaccagaac gttctgggaa aaaagatcct
     4921 ttagagaacg aaggctataa cctctacttt aacaatcgtt atattaaaaa tatgccgatt
     4981 gttgcgaatt tatcacatgg accagttggc tatataggac cacgaggttt agtattggag
     5041 caactccaat tgatggttaa tcaattagct tttttccatt cttaccatga tgttcaattt
     5101 attactattg ttccagagga agagatggat aagtggtctt ggatgcgttg gttgccacat
     5161 gcaaccttac aagatgtcaa tgttcgtggc tttgtttata atcaaagaag ccgtgatcaa
     5221 gtgctcaaca gtcttaacca aattttaaaa ttacgtcgca cacaacgcga agataaatct
     5281 gccaaagaag ggactttatt ttctccgcat tatgtcgtta ttgtaactga tgaaaagtta
     5341 attttagatc acgtcatcat ggaatttttt acggaagatc ctacagaatt gggatgttct
     5401 ctcatctttg ttcaagatgt tatgagttca ctttcagaaa atatcaaaac cattattaat
     5461 attaaagacc gtaatactgg tcagttagtt atagaagaag gagagcttaa agaaacagat
     5521 tttgaattag atcatttctt agaggattat gacaaggaaa atatttcacg tcgcctagct
     5581 ccgttaaatc acctacaaaa tcttaaatct tcaataccag aagcagttac ttttatggaa
     5641 atgtatcaag cagaagaatt tgaagatttg catgttcaag agcgttggat aagccatgct
     5701 ccgtataaaa gcttagctgt tccacttggc ttacgtggtc aggatgatat tgtttatcta
     5761 aacctacatg aaaaagctca tggtcctcat ggattagttg caggaacaac gggatcaggg
     5821 aagtcagaaa ttatccagtc ctatatcctt tctttagctg ttaatttcca tccacacgat
     5881 gtagctttcc ttctcataga ctacaaaggt gggggaatgg caaatctctt taaggacctg
     5941 ccacacttac ttggcaccat caccaactta gatggtgccc aatcgatgcg tgctttggtt
     6001 tctattaatg ctgaattgaa acgtcgtcaa cgtttatttg ctaaagctga tgttaatcat
     6061 attaaccagt atcagaagaa atacaaactt ggagaagttt cagaaccaat gcctcatctc
     6121 ttcttaatct ccgatgagtt tgccgaattg aaatccaacc aacctgagtt tatgaaagag
     6181 cttgtctcaa cagcgcgaat tggtcgttcc ctagggattc acttaatcct agcgacccaa
     6241 aaaccatctg gcgtggtaga tgaccaaatc tggtcaaact cacgcttcaa actagctctt
     6301 aaagtagcag accgcggcga ctccatggaa atgctccata ccccagatgc cgccgagatt
     6361 acacaagcag gacgtgccta cctccaagtt ggtaataatg aagtctatga actcttccaa
     6421 tcagcatggt caggagcaga ttaccaacct gaaaaagacg accaagggat tgaagaccat
     6481 accatttatt ccatcaacga ccttggccaa tatgaaatcc taaatgatga tctttcaggc
     6541 ttagaccaag ccgaaaatat taaagaagta cctacggaat tagatgccat cgttgaaaac
     6601 attcaaactt taaccaaaga aatgggcatc tcagacttac ctcaaccgtg gctaccgcct
     6661 ctaagcaatc agattgcagt aaccgatctc cgtaaggaag aatcagtaga tctctggagt
     6721 aaagccccat cttacaaagt tgttctgggc tttatggata taccaagcca acaagcacaa
     6781 gaagtagctt accacgattt tgaagatgat gggcatttgt caatttttgc aggtccaagt
     6841 atggggaaat ccacagccct tcaaactgta acattggact tagcacgtca caatagtcca
     6901 gaatttttaa acctttatct ctttgatttt ggtaccaatg gcctcttacc attaagacgt
     6961 ttgccacatg tagcagattt ctttaccatt gatgatgacg aaaaaatagc caagtttatc
     7021 agcagaatca aaacggaaat gtctgaccgt aaaaaagccc tcagtcgcta caatgttgca
     7081 acagcaaaac tttaccgcca ggtatctggg gaaaccatgc ctcaaatcct gattgttatt
     7141 gacagttatg aagggctacg ggaagctcaa acactgacta acttagaagc ttgtttccaa
     7201 aatatttcgc gtgatgggtc gtcacttggc atttccttag ttatctcagc tggtcgtatg
     7261 gcagctttga ggtcgtcctt aatggcaaac cttaaagaac gtatcgctct taaattaaca
     7321 gatgactctg aatcacggac acttgtcggt cgtcaccaac acatcatgga agatattcca
     7381 ggacgcggat taatcaaacg tgatgacatt gaagtgttac aagtggctct cccaacagaa
     7441 ggcactgaaa cctttgacat catcaataac atccaaaacg aatctgacgc catgaacagc
     7501 aaatggactg gaccaagacc aaaagcaata ccaattgttc cagaaacact tacctttgag
     7561 gagtttaagg catatgagga tgttaaaaaa gcaagtgaga ctaatcagat ttcacttggt
     7621 attagcttaa aagaagttga taattgtagc attccacttt cgaaattcaa acagttactt
     7681 tttacttctg acaatccaga taatttgtct aacttgacgc gcttcttagt taaatctgtt
     7741 tcattgatta aagatagtaa ggtttatctt ttagacactg cttctgatta tgatgatttg
     7801 acagaacaac aaattgaata tatcaataat cgaagtgaaa tagcaggaca tttggaagct
     7861 attgtttcag aaatctattt aagagaagaa tctggtaaaa tagaaccatg gtttgttatt
     7921 attcctgata tgaatagttt tgtggcagaa acaaatatgg ataaagaaca gattttattc
     7981 ttacttgact cggctcacaa atatcatatt tatttcttat ttggtggctt agcatcatac
     8041 cttatgacta atatctcaga tgttccaaaa gctattagaa cacaggcaca gtatgttctc
     8101 ttgggaatga gagttatgga tcagtcggta ttaccgaaaa catataattc aaaagaacct
     8161 tatttgaaac cagatatgat ttatattcat gatagaagac aagagacaat gcttaaaatc
     8221 actcaagaaa tagaaatgga gcattgagta tatgaaaaaa caaagactat tactgctttt
     8281 tggaggctta ttaataatga taatgatgac agcatgtaag gattcaaaaa tcccagaaaa
     8341 ccgcacgaaa aaggaatacc aggcagaaca gaattttaag tcatacttta aatatatatc
     8401 agataaaaat aactatttag ataatataaa agtttattac ttttctataa gtatttctaa
     8461 agatgtacaa gataaagtca gtgaaacaac aacttgttca tatagactag aaaagcaaaa
     8521 gaatcaagag ttcattggta attttgaaca tgaagttagt gaatctagtc aatattcaac
     8581 cgaagttaaa aatcaaatac agtatccaat ccagtataaa gataattcaa ttcgttttac
     8641 tgaaaaaaca ccgtcagaac gttatgatga gtttgttttt agttcatttg attcttcatt
     8701 attaaaaaaa tataaaatat atgattactt actaaaacat cccgaaactg aattaaaagg
     8761 tgtttcctat aagattccta taaattctga aattgtagcc ccttttataa agcaatttaa
     8821 tataaaaaat cctaaaaaat catctatttc ggttacaaaa acggaaagta aagaatatta
     8881 ttatacaatc agtattgata ctgattctga gatatattct atattcgaag gtattacatt
     8941 agaggatgta aaggagtttt ctaaatgagt ccaagtgaga aattagatag aagaattgat
     9001 tcttcatcag acctctccca gtatgtatat gattttgaaa agcaatatac agaatatatg
     9061 aagaaggggt attctgaaaa aaatgcgagt atacaagcac gacgaaccct gaatgaaata
     9121 ctacaagaaa ataatccagg attgagatct agcgacgcca tgtatgataa agaaacaggt
     9181 attgcagcta ttgctgtaaa agataaaaat actggtgaga cttatatagc ttatgcgggt
     9241 actaataagg atgctgatgg ggcaaccgac tttatttcag atgcaaatat cggtgctaat
     9301 aactctgctt atttaaaacg attaggagac aaagctacta gcttttatga tcgtgttcaa
     9361 attaatggcg ataatattac tgtcacaaca ggtcattctt atggtgactt tctcgcttct
     9421 agagtggcaa ttgagctaat tccattcttt attaacttta gaaaagttaa tgattcattt
     9481 cacttttgtt attagcttgt gatcacttat aaagtgacag ctagttcatg aaatttctta
     9541 ggagttttct ggctttaaag gtaacctatg atttaaaaaa catctcatgg aattcaatgc
     9601 ggaatcctca acagtctttt gtggactaac agaagttcag gatgtcgttt cggcaggcct
     9661 taactattct cagaaggttt tggaaatttc aatggtacat ttgatacctc aggtaaggga
     9721 ttagaatgga caaaaacaat tcaaacaggt atgaaaaaac gagaagagaa aatcaaatac
     9781 cctcatttta caattgttga cataaagtaa ttaaacaaaa tccggaaaat ttatattaag
     9841 taatttttct aaaaagtcat ataggcatac ttttaaagtt cctctaaact taataaggaa
     9901 gaaacgtttt gaaaaaagat aaatatattg cattgaattg gttgtgtgcg atagccacgc
     9961 tatactatta agttattaca aactatgaat tacacctcaa atgttggaga taaaacctat
    10021 cgtttggggt gttttttggt aaaatagaat gaaaagagtt actctgtgtc attactaagt
    10081 taaaaaatta tactaactta attattaacg ttttattgac tgagttactc ttttttaaaa
    10141 aatgtatatc attccagctg gttactcacc tgaacgttta gactcagaag tccttacagc
    10201 aaagattgaa gatgatgaag ccttattgcg gaaaattcaa gaccttaaag atcatttaga
    10261 taaaaatacc aaaatcttgg cttacgcttt tgatggcttg gaagtcagag tgaaagctag
    10321 actaagaaaa aatcgtaaga aactcaaaaa ccttatagaa ttcaatgcca aatctacctc
    10381 agtctttaat gaattgaagg attattcaaa tgctgtatcg tctggtttaa cctttgtatc
    10441 taaaggcttt agtagtttca atggccagtt ctcttcagcc tcaaatcttg attggcaaaa
    10501 gactattaag acaggaatga gaaaacgaga agaagatgct aaacagttga gaaaaaaagc
    10561 tttagaagat tcaaataagc tcagaaaaat tgcatcaaaa aggggttact ttgaataatg
    10621 cagttgaatt tgctaaagaa cagaacaaac ccctaaaaat aaaaagataa ccatagataa
    10681 atatggtcgt gtaaaaatgg ataaaacttt tctttatcga aaagctaaag atggaaaatc
    10741 tgcatatatt tattctgaag ggagtgatca atataaagaa ctaactggga aagaatttga
    10801 tcctaaaact acaaatatta tgaaaggtag aaaattaggg acagatggta agatatcaat
    10861 tgattttggt gagattagta actcaggaaa aaatgcagct attaagtccc ttaaaaatga
    10921 aagtccttga attaaatccc aaaaatgctt ttaaaggatg gaatgagtta agcaaaatag
    10981 ctaaaactgg gaaagtacta ggatggacag gtaaaattgc taatatttac atgaatggaa
    11041 aaaaagatct ttttgatgat aaaacatctt caatagatca aaaaatcaaa aactttgcta
    11101 taggttcagg tgtagatgca ttgtccggtg cagcaatagg gactttggta ggaggacctg
    11161 tgggaaccgt tgttggagca ggtgcagggc tggcatttga ttttgctttg aattttaaag
    11221 ttaaggagtt gggtaatgaa tcaattacag gttttgctaa aaaaggttta aaaaatcttg
    11281 caggaggatt aggattttga aaaaaagtat aaatgtaagt aggtatcatg gacctttaat
    11341 tgaatttgag gttgatgcaa gttatttaag aattttttca tatatcggtt tacctttatc
    11401 attgttttgg tattttgtta ctcataacag tgatgacttt ggaatattaa aatcgatatt
    11461 tttaatttgt attatagtca atttaatcgg gctcatatta ggattaatac aaaagtttac
    11521 taaaaaactt atcaaatctc tatcattttt tgtactttct tagcgtttct ttcgattttc
    11581 ttagggttag atacaatatg tttaatggta tttgtttcgg atgggtcccc tgttgatgcg
    11641 aatacgttgt attttggtaa gggtactcca atttttatga taatgaacgt tacgatattt
    11701 atcatttcta ctttgatcta ttcctactat tatttaccaa aaaatcaagg gaaagtttgg
    11761 aaatttaatc aatggaataa cgaaaagagt cgagaacaac aatacataag taattttatt
    11821 gttacatttg gtctgatatt gttagttact ccatttttaa agtatggtat ctataacagt
    11881 tttggagttt taatcagttt actatttaca gcaaccttac catcattaat tgttgattca
    11941 ctttatgcgg cattttatgt ccataagcat cctgagtatg aataatcaac ttataaagaa
    12001 catcgaaaac acatgatacc ttttataaga cagaaactat aaattcagat aaaacatctt
    12061 cgcttgataa gtcagagact ttaaagatag aggataacaa gctattttag acaaaaaatt
    12121 agaaatttca caatagattc tagagtagat actttattag gtgctggaag gttattgggc
    12181 acttgattgt gattggactg gacaaaacaa accagatcaa tcagtaactg gttttgcaaa
    12241 aaagaattaa aagatttatt tggaggggaa aatttttgaa acagagcaat catagatacc
    12301 atggaccgtt aattgagttt gaaatagaat attcatatat taaaattttt ccatggattg
    12361 gactatttat atcttgcata gggtt
//
LOCUS       DATRSG010000064        11634 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRSG010000064 DATRSG010000000
VERSION     DATRSG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.064"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            77..148
                     /locus_tag="VBD89_001673"
     tRNA            77..148
                     /locus_tag="VBD89_001673"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Glu,seq:ttc)
     gene            211..729
                     /gene="rimM"
                     /locus_tag="VBD89_001674"
     CDS             211..729
                     /gene="rimM"
                     /locus_tag="VBD89_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO7136184.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            716..1471
                     /gene="trmD"
                     /locus_tag="VBD89_001675"
     CDS             716..1471
                     /gene="trmD"
                     /locus_tag="VBD89_001675"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO7136185.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            1452..2444
                     /locus_tag="VBD89_001676"
     CDS             1452..2444
                     /locus_tag="VBD89_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7136186.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            2581..3651
                     /locus_tag="VBD89_001677"
     CDS             2581..3651
                     /locus_tag="VBD89_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7136187.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            3664..4587
                     /locus_tag="VBD89_001678"
     CDS             3664..4587
                     /locus_tag="VBD89_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO7136188.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            4799..6433
                     /locus_tag="VBD89_001679"
     CDS             4799..6433
                     /locus_tag="VBD89_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO7136189.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFGGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLPKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            6577..7662
                     /locus_tag="VBD89_001680"
     CDS             6577..7662
                     /locus_tag="VBD89_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7136190.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPFDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGI"
     gene            7687..7812
                     /locus_tag="VBD89_001681"
     CDS             7687..7812
                     /locus_tag="VBD89_001681"
                     /inference="COORDINATES: protein motif:HMM:NF014445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEO7136191.1"
                     /translation="MLRFKQNFNGYIIRKMGTFNYYPSPFKYKGIQLLKKVLKRN"
     gene            7877..8686
                     /locus_tag="VBD89_001682"
     CDS             7877..8686
                     /locus_tag="VBD89_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7136192.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            8683..9594
                     /gene="pfkB"
                     /locus_tag="VBD89_001683"
     CDS             8683..9594
                     /gene="pfkB"
                     /locus_tag="VBD89_001683"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO7136193.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            9591..11555
                     /locus_tag="VBD89_001684"
     CDS             9591..11555
                     /locus_tag="VBD89_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7136194.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAIVSGVLFGLFRKTK"
BASE COUNT         3858 a         1748 c         2390 g         3638 t
ORIGIN      
        1 gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt cgaatccagc
       61 taccccagtt tcacatggtc cgttggtcaa ggggttaaga caccgccttt tcacggcggt
      121 aacacgggtt cgaatcccgt acggactatc gtggccagtt gcattttagc ggctggtttt
      181 tcttttattt tcaaaaatat cggagttact atggaatact ttaacgttgg aaaaattgtc
      241 aatacacaag gattacaagg cgaaatgcgt gtattaagcg tgactgactt tgtagaagaa
      301 cgctttaaaa aaggtcaagt gttagcttta ttcgatgaaa agaatcagtt tgttatggat
      361 attgaaattg ctagtcatcg taagcagaag aattttgaca ttataaaatt caaaggaatg
      421 tatcatatta atgatattga aaaatataaa ggtttcacac tgaaggtggc agaggatcaa
      481 ctgagcgacc ttaaagatgg tgaattttat tatcatgaaa ttattggctt agatgtctat
      541 gaaggggaag agctgattgg aaaaatcaaa gaaattttac aaccaggagc aaatgacgtt
      601 tgggttgtgg aacgtcatgg aaaacgtgat cttttattac cctatatccc gccagtagta
      661 ttagaagtgg acctttctaa tcagcgtgtt caagttgaat tgatggaagg tttagatgat
      721 gaagattgat attttaactt tatttcctga aatgtttgcc cctctggaac attctatagt
      781 tggtaaggca aaagaaagag gacttcttga aattaattat cacaatttca gagaaaatgc
      841 tgagaagtct cgacatgttg atgatgagcc ttacggtggg gggcaaggga tgcttttacg
      901 tgctcaacct atttttgata caattgataa aatagatgct caaaaagcac gtgttatttt
      961 gctagaccca gcgggcagaa catttgatca ggattttgca gaagaactat ccaaagaaga
     1021 tgaattaatt tttatttgtg gtcactatga aggttatgat gaacgtataa aatcattagt
     1081 aacagacgaa gtatctttgg gagattttgt tcttacggga ggagaattgg cggctatgac
     1141 aatggtcgat gctaccgttc gtctgattcc agaagttata ggtaaagaaa caagtcatca
     1201 ggatgattca ttttcatcgg gcctattaga atatcctcag tatacgcgcc catatgatta
     1261 tttaggcatg acagttcctg atgttctcat gagtggtcat catgagaaca tccgaaaatg
     1321 gcgtttagaa caaagtttgc gaaaaacact agaacgtcga ccagacttgt tagaaaatta
     1381 tgcaatgacg gatgaagaga gacttatctt agagaaaatt aaaacagaaa tagaaagaac
     1441 agatacagta aatgaacaaa acaatttatg atatcactat tgtaggaggc ggaccagtag
     1501 gtctctttgc tgctttttat gctggactac gcggagtatc cgttaaaatt attgaaagcc
     1561 tttcggaatt aggaggccag ccagctatcc tttatcctga gaaaaaaatc tatgatattc
     1621 ctggttatcc ggtgattact ggacgtgaat taattgataa acatattgaa caattagaga
     1681 gatttaaaga ctctattgaa atctgtttaa aagaagaagt tttatcattt gaaaaagtag
     1741 atgatgtttt cacaattcaa acagataaag atcaacattt gtcacgtgct attgtttttg
     1801 cttgtggcaa cggtgctttt gctccgcgtt tactaggttt ggaaaatgaa gaaaattatg
     1861 cagataataa tttattctat aacgttacaa agttagaaca gtttgcaggt aaacatgttg
     1921 ttatttgtgg tggaggcgac tcagctgttg attgggccaa tgaattagat aagattgcag
     1981 ctagtgtagc gattgttcat cgtagagatg cttttagagc gcatgagcat agtgtggata
     2041 tactaaaggc atcaggtgtt agaattttaa ccccttatgt cccaattggt ttaaatggag
     2101 atagtcagag agttagcagc ttggtcgttc aaaaagttaa aggtgatgaa gtaattgaat
     2161 tacctttaga caacttgatt gttagttttg gcttttctac ttctaataaa aatttacgtt
     2221 attggaattt ggattataaa cggtctagta tcaatgtttc ttcacttttt gagacgactc
     2281 aagaaggcgt gtatgctatt ggagatgctg ctaattatcc aggaaaggta gaattgattg
     2341 caacaggata tggggaagca ccagtcgcta taaaccaagc tatcaattat atttatcctg
     2401 atcgagataa ccgtgtagtc cattcaacat cattgataaa ataactataa ataaaactga
     2461 taagtttata aaaaaggatt atatatattg ttaaaaaaat aacaaaactt cctatttatt
     2521 ttgtgatata attaactttg tcgttatgaa tgatattaaa taaatgaagc gaggtatttt
     2581 atgacaaata cggtaacacc aaaagagacg gctggctctt ttatcaataa agtgttaggt
     2641 gggacagcaa ctgcaatcgt tgtagcactc atcccaaatg ctatcctagc cacttttttg
     2701 aaaccatttt tatcttacgg acttgctgca gagttcttac acatcgtgca ggtattccaa
     2761 tttttcacac caattatggc aggatttttg attggtcaac agtttaaatt caccccaatg
     2821 caacagcttg ctgttggagg tgctgcatat attggttctg gtgcgtgggc ttacacagaa
     2881 gttattcaaa aaggagttgc tactggtagt ttccaattgc gtgggattgg tgatctcatc
     2941 aatatgatgc taacagcagc tttagcagtt ttagcagtta agtggtttgg taataaattt
     3001 ggttccctaa ccattatctt gttacctatt atcataggta caggcgtagg ctatttagga
     3061 tggaaattac tcccttacgt ttcatatgtg acaacactta ttggtcaagg aattaattct
     3121 tttactactt tacaaccaat tgcgatgtca attctaattg cgatggcttt ttcaatgctt
     3181 attgtaagtc ctatttcaac agtagcgatt ggtctagcga ttggtcttaa tggtatgtca
     3241 gcaagtgcag cttcaatggg agttgcttca acaacagcag tgctagtttg ggcaacgatg
     3301 aaggctaata agtctggagt tccaattgct attgctttag gtgcaatgaa gatgatgatg
     3361 ccaaatttcc taaagcatcc agtaatggcg attccaatgc ttatgactgc tacagtaagt
     3421 tctctaacag tcccattgtt taaattggtg gggacaccag cttcgtctgg ttttgggctt
     3481 gttggtgcag taggtccgat tgcttcattt gaagcaggag caagtatgtt gattgttatc
     3541 ttatcttggc tcgttattcc ttttgcagta ggttttgtat cacacaaaat ttgtaaagac
     3601 attctgaaac tttataaaga cgatattttc gtttttgaag gtcaaaatta atttggaggt
     3661 tgtatgttag tttatattgc tggttcaggc gctatgggat gtcgttttgg atatcaaatt
     3721 tctaaaacaa atcatgatgt tattttactt gataattggg cagaccatat tatggctatt
     3781 aaagaaaatg gcttgaaggt aacaggtgat acagaggacc ttgttaaact tcctatcatg
     3841 aagccaactg atgcgacaga agaagcagat ttaattattt tgtttactaa agcaatgcag
     3901 ttgccaaata tgcttcaaga tattaaaaaa attattggta agaaaacaaa ggtcttatgt
     3961 cttttaaatg gattaggaca tgaagatgtc attcgtcaat atatccctga acacaatatt
     4021 ttgatgggag taacagtttg gactgcaggg ttgaaaggac cagggcatgc tcatctggaa
     4081 ggagttggtt ctgtaaatct tcaaagtatc gatcctaaca atcaagaagc aggtcatcgc
     4141 gttactgaat tgcttaatga agcaaaatta caggctactt atgacgaaaa tgttttgcca
     4201 aatatttgga gaaaagcttg tgttaacgga acgatgaatt caacttgcgc cttgctagat
     4261 tgtacaattg gtcaactctt tgctagtgaa gacggcgtaa atatggtaca tgaaattatc
     4321 catgaatttg ttactgttgg aaaagcagaa ggtgtagagc ttgatgaaga agaaataaca
     4381 aagtacgtta tggatacatc tgttaaagca gcccatcatt acccttcaat gcatcaagac
     4441 ttagttcaaa atcagcgctt aactgaaatt gatttcttaa atggcgcagt gaataaaaaa
     4501 ggtgaaaacc taggcattga tacaccttac tgtcgattaa tcacacaatt aattcacact
     4561 aaagaaaatg tacttagcat aaaataataa tcaaccggga gaatagtgcc cggttttttg
     4621 atatgcttaa tgataatgat tttgttatac taagaataat aagagttggg tatttttttc
     4681 gagaagtatt tacagtataa aatattacat ttgtattaca aatatttcta aaatatgttg
     4741 acatgttacg aaaaggatat tataatagta gtgtaaataa ataaggaagt aggtgtttat
     4801 gatgaaaaaa ggacaagtaa atgatactaa gcaatcttac tctctacgta aatataaatt
     4861 tggtttagca tcagtaattt tagggtcatt cataatggtc acaagtcctg tttttgcgga
     4921 tcaaactaca tcggttcaag ttaataatca gacaggcact agtgtggatg ctaataattc
     4981 ttccaatgag acaagtgcgt caagtgtgat tacttccaat aatgatagtg ttcaagcgtc
     5041 tgataaagtt gtaaatagtc aaaatacggc aacaaaggac attactactc ctttagtaga
     5101 gacaaagcca atggtggaaa aaacattacc tgaacaaggg aattatgttt atagcaaaga
     5161 aaccgaggtg aaaaatacac cttcaaaatc agccccagta gctttctatg caaagaaagg
     5221 tgataaagtt ttctatgacc aagtatttaa taaagataat gtgaaatgga tttcatataa
     5281 gtcttttggt ggcgtacgtc gatacgcagc tattgagtca ctagatccat caggaggttc
     5341 agagactaaa gcacctactc ctgtaacaaa ttcaggaagc aataatcaag agaaaatagc
     5401 aacgcaagga aattatacat tttcacataa agtagaagta aaaaatgaag ctaaggtagc
     5461 gagtccaact caatttacat tggacaaagg agacagaatt ttttacgacc aaatactaac
     5521 tattgaagga aatcagtggt tatcttataa atcattcaat ggtgttcgtc gttttgtttt
     5581 gctaggtaaa gcatcttcag tagaaaaaac tgaagataaa gaaaaagtgt ctcctcaacc
     5641 acaagcccgt attactaaaa ctggtagact gactatttct aacgaaacaa ctacaggttt
     5701 tgatatttta attacgaata ttaaagatga taacggtatc gctgctgtta aggtaccggt
     5761 ttggactgaa caaggagggc aagatgatat taaatggtat acagctgtaa ctactgggga
     5821 tggcaactac aaagtagctg tatcatttgc tgaccataag aatgagaagg gtctttataa
     5881 tattcattta tactaccaag aagctagtgg gacacttgta ggtgtaacag gaactaaagt
     5941 gacagtagct ggaactaatt cttctcaaga acctattgaa aatggtttac caaagactgg
     6001 tgtttataat attatcggaa gtactgaagt aaaaaatgaa gctaaaatat caagtcagac
     6061 ccaatttact ttagaaaaag gtgacaaaat aaattatgat caagtattga cagcagatgg
     6121 ttaccagtgg atttcttaca aatcttatag tggtgttcgt cgctatattc ctgtgaaaaa
     6181 gctaactaca agtagtgaaa aagcgaaaga tgaggcgact aaaccgacta gttatcccaa
     6241 cttacctaaa acaggtacct atacatttac taaaactgta gatgtgaaaa gtcaacctaa
     6301 agtatcaagt ccagtggaat ttaattttca aaagggtgaa aaaatacatt atgatcaagt
     6361 gttagtagta gatggtcatc agtggatttc atacaagagt tattccggta ttcgtcgcta
     6421 tattgaaatt taaagctaaa tctttttaga tttagcttta ctaatattat gtgtgttgga
     6481 aaaaacagtg gagatttacg tcaaaattat attatttgga tagtatcgaa tagatagaat
     6541 gtggagaaaa gtaggaaata gttaagaaag gtatttatga cgcttcgaga attaacaata
     6601 gaagaattta aagaacattc aggaaattat gattcacaat catttttaca aacacctgag
     6661 atggctaaac ttttagaaaa acgcggctat gatgttaggt atttgggata tcaagtagaa
     6721 aataaactag agataatcag tttatcttat attatgccag tcactggtgg ttttcaaatg
     6781 aaaattgatt caggaccagt tcattcaaat tctaagtatc taaaacaatt ttataaagca
     6841 ttgcaaggct atgccaaatc caacggtgtt ctagaattaa tagttgagcc ttttgatgat
     6901 taccaattat tcactagttc gggagttcct agtaatcagg gaaatgataa tctgattgaa
     6961 gattttacca gttcaggtta tcaccatgat ggtttaacaa ctggttttac tggtaaatat
     7021 ttatcttggc actatgttaa aaatttagaa ggtgtcactt ctgaaacgtt actatcttca
     7081 ttctctaaga caggacgagc tttggttaag aaagcaatgt cttttggaat caaggttcgc
     7141 gttcttaaac gtgatgagct acatttattt aaagagataa caacttctac gtcaaataga
     7201 cgtgattata tggataagtc cttagattat tatcaagatt tttacgatag ctttgaaggc
     7261 aaggctgaat ttgtgattgc cactttaaat tttagagaat acgaccataa cttgcaaata
     7321 aaagctgaag cattggaaaa taagcttaaa ttgcttgatg aacgttttag ggaaaatgca
     7381 gactctccta agtatcatag gcaacgctct gaaataatta atcaactggc tagttttgaa
     7441 acacgacgac aagaagttca gtcatttatt caaaagtatg ataaccaaga cgttgtttta
     7501 gctggtagtc tatttgttta tagtcttaag gagacagttt atttcttttc aggatcctac
     7561 acagaattta ataaatttta tgcaccagca gttcttcagg aatatgttat gcaagaggct
     7621 ctcaaaagag gttctacttt ctataacctc ctaggtattt agggaacttt tgatggttca
     7681 gatagtattc ttcgctttaa acagaatttt aatggttata tcattcgtaa aatgggaact
     7741 tttaactatt atccaagtcc ttttaaatac aaaggaattc agcttcttaa aaaggttttg
     7801 aagagaaatt aaagtatttg taaaagctaa tctttagatt aggctttttt agtttattga
     7861 tagtttttgg aagaaaatgc ttgacattga aagaaaaaga gatataatta aattgcaaac
     7921 gatttcaatt caaggaggga ctatgctaaa gtcaaagaga aaagaaatta tcttatcacg
     7981 acttgagcaa aataagagtg ttactttaga tgaattaacg tctatcttag agacttctga
     8041 atctacagtt cgcagggatt tagatgaact tgaatctgaa ggttttttaa aaagggtgca
     8101 tggtggtgct gaattaccat actcacttgg ccaggaatta agtaatcaag aaaaatctat
     8161 caaaaacgtt caaaaaaaat tggatattgc tagacaaact gcaaagctga tagcaaagca
     8221 agatgttata tttattgatg ccggaactac gacagaatta ttaattgatt ttctccctca
     8281 cgagcaatta acggtggtga cgaattctat tcatcatgct gcaaagttag ttgatagagg
     8341 gataaaaaca atcattatcg gaggagcggt taaacattca acagatgcta gtattggcca
     8401 ggtagccatt aatcagatta ggcagataac ggttgacaaa gcttttctgg ggatgaatgg
     8461 tatagatgaa gtttatttaa ctacaccaga tttagaagaa gctgctatta aagaagctat
     8521 tattaataat agtcaacaaa cctttattct aatggattca tctaaaattg gacaagttac
     8581 atttgctaaa gtgaaagaga taaatgacat taatttagta actaataaga ctgattcaga
     8641 acttatgaca ataataaaag agaaaatgaa ggtgattcaa gtatgattta tacagtaaca
     8701 ttaaatccat cgattgattt tattgttcgt ctagatacac tattgttggg tagtgttaac
     8761 cgtatgacta gcgatgataa atatgtaggt ggtaaaggaa ttaatgttag tcgcatatta
     8821 aaacgcttaa aaattgacaa tactgctaca ggttttatcg gtggatttac aggacatttt
     8881 gttgaagatg gacttgtatc agaaggtatc aaaactgact ttgtttctgt taatgaagat
     8941 acacgtatca acgttaaagt aaaagcaaaa attgaaactg aaatcaatgg cgggggtcct
     9001 aggataacaa atgaacaact tcatcgtcta gagaaacttc tttcgagact aacacctgag
     9061 gacacagtag ttttcgcagg ttcagcacca gctagccttg gaaataaggt ctataataca
     9121 ttgattccaa ttgctaaaaa gacaggagcg gaagttgttt gcgattttga aggtcagaca
     9181 cttttagacg ctcttgctta tcagcctctt ttagtaaaac cgaataacca tgaattagca
     9241 gatatttttg gcgtagagct agaaggtctg cctgatatcg aaaaatatgc acataagatt
     9301 ctagataaag gagctaagaa tgttatcgtt tctatggcag gggatggtgc attgttagta
     9361 actccagaag cttcatattt tgcaaaaccg attaagggtg aagtgaaaaa ttccgttggg
     9421 gctggtgatt caatggtagc tggctttaca ggagaatttg ttaagtcaaa aaatccagta
     9481 gaagcactaa aatggggcgt agcttgtggt acagcaacca ctttctcaga tgatttagca
     9541 acagctgagt ttatccaaga tatttataat aaagttgagg tagaaaaatt atgaaaatcc
     9601 aagacctact aaaaaaagaa gttatgatta tggatttaaa agcaaccagt aaagaggctg
     9661 caattgatga aatgattaca aaattagttg acacaggtgt tgtgacaaac tttgctattt
     9721 ttaaagatgg tatcatgaag cgtgaggcac aaacatcaac tggattaggt gatggtatcg
     9781 ctatgccaca tagtaaaaat gctgccgtta aagaagctac tgtccttttt gcaaaatctg
     9841 catcaggtgt tgattacgaa gcacttgatg gacagccaac tgatttattc tttatgattg
     9901 ctgcgccaga tggtgctaat gatacgcatt tagctgcctt ggcagaacta tctaaatatc
     9961 ttttaaaaga agggttcgct gatcaattac gtcaagcaaa aacgccagat gacattattg
    10021 ctacttttga tagtaatagc atttctcaag aaacagtagc tccacaaaca gtacaatcaa
    10081 caagtaaagg ttcagattat attgttgcag taacagcttg cactacggga attgcccaca
    10141 cgtatatggc agaagaagct ttaaagaaaa aagcagctga aatgggtgta ggtattaaag
    10201 ttgaaacaaa tggcgcttct ggtgtaggaa ataagttgac ttcaagtgat attgcaaggg
    10261 ctaagggtgt tattattgca gcagataagg ctgtagagat ggatcgtttt gatggcaaac
    10321 ctttggtatc acgtccggta gctgatggta ttaaaaaaag tgaagactta attaacatta
    10381 ttctcgataa taaggcacaa acctatcatg caaaaaatca aaatgataaa caatcaggtg
    10441 aatctgatgg aaaatcaggc ttaggctctg ctttttataa acaccttatg ggtggtgtat
    10501 cacaaatgtt accattcgtt attggtggtg gtattatgat tgcgattgct ttcctttttg
    10561 ataacatact tggtgtacca aaagaccaat taagtaattt aggttcatac catgaaattg
    10621 cagccttatt caaaaatatc ggtggcgcag catttgcatt tatgttacca gtattggcag
    10681 gttatatcgc ttattcaata gctgaaaaac caggactagt agctggcttc gtagctggtt
    10741 caattgcatc aagtggactg gcattcggta aagtaccttt tgcagaaggt gggaaagcaa
    10801 ccttagcatt agcaggtgtg ccatcaggtt tcttaggggc tttagtaggt ggtttcttag
    10861 ctggtggagt tatcctacta cttagaaaat tactttcagg tctcccaaaa tcgcttgaag
    10921 gtattaaatc aattcttcta tacccattgt taggtgtttt gattacaggt ttcttgatgt
    10981 tgctcgtaaa cattccaatg gcagctatca acacagctct taacactttc ttacaaggtt
    11041 tatctggtag ttcagcagtc ttgatgggct tactagttgg tggtatgatg gctgtagata
    11101 tgggtgggcc tgttaataaa gcagcttatg tttttggtac aggtactctt gcagcaactg
    11161 ttgcaaatgg cggctctgtt gttatggcag ctgtaatggc tggaggaatg gttccaccgt
    11221 tagcagtatt tgtggcaact ttattattta aagataaatt taataatgaa gaacgtcaat
    11281 ctggtctaac aaatatcgtt atggggttat cttttatcac tgaaggtgct attccgtttg
    11341 gtgctgctga cccagctcgt gcaatcccta gctttattgt aggttcagcc ttaactggag
    11401 cacttgttgg tttagctggt atcaagttga tggctcctca tggagggatt tttgtcatcg
    11461 cattaacaag taatccttta ctttatattc tatttattct aattggagcg atagtctcag
    11521 gtgtactctt tggcttgttc cgtaaaacaa aatagttaat ctcaacttat ccaaatttac
    11581 attgaataaa aggtgaataa actatcgtta ttgggtctat tcgccttttt atgt
//
LOCUS       DATRSG010000065        11028 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRSG010000065 DATRSG010000000
VERSION     DATRSG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.065"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1002)
                     /locus_tag="VBD89_001685"
     CDS             complement(<1..1002)
                     /locus_tag="VBD89_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7136195.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVAGGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     "
     gene            complement(986..1657)
                     /locus_tag="VBD89_001686"
     CDS             complement(986..1657)
                     /locus_tag="VBD89_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7136196.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            complement(1688..2374)
                     /locus_tag="VBD89_001687"
     CDS             complement(1688..2374)
                     /locus_tag="VBD89_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136197.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            complement(2374..3462)
                     /locus_tag="VBD89_001688"
     CDS             complement(2374..3462)
                     /locus_tag="VBD89_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136198.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDRLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            3644..4630
                     /locus_tag="VBD89_001689"
     CDS             3644..4630
                     /locus_tag="VBD89_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7136199.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            4623..5534
                     /gene="rbsK"
                     /locus_tag="VBD89_001690"
     CDS             4623..5534
                     /gene="rbsK"
                     /locus_tag="VBD89_001690"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO7136200.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            5509..5907
                     /gene="rbsD"
                     /locus_tag="VBD89_001691"
     CDS             5509..5907
                     /gene="rbsD"
                     /locus_tag="VBD89_001691"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO7136201.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANSDQLSRLLAKLDNSVNIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            5923..7401
                     /locus_tag="VBD89_001692"
     CDS             5923..7401
                     /locus_tag="VBD89_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO7136202.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            7403..8344
                     /gene="rbsC"
                     /locus_tag="VBD89_001693"
     CDS             7403..8344
                     /gene="rbsC"
                     /locus_tag="VBD89_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO7136203.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLTPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            8397..9365
                     /locus_tag="VBD89_001694"
     CDS             8397..9365
                     /locus_tag="VBD89_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO7136204.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(9405..10859)
                     /locus_tag="VBD89_001695"
     CDS             complement(9405..10859)
                     /locus_tag="VBD89_001695"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO7136205.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
BASE COUNT         3555 a         2066 c         1925 g         3482 t
ORIGIN      
        1 aacaattata cgtttaacta ttgctagtcc taatccattt ccttgttgat tatgcgactg
       61 atccgcttgg taaaatttat caaatatatg ctgaacatca tattgagaaa ttcccctacc
      121 tttatcagaa ataataactc tcagataatg attattagtt tcttccattc gtacacttaa
      181 gtctacaata tcctcggaat attttattgc attatctaaa aggtttatcc aaacttgcat
      241 cagcaagtca gagtttgagt aaacagtata tggttttgaa tccagttgaa aatttatcct
      301 tttagcttgc caacgttctg ttaaactaat cattgcttga cgaatttgtt catctacacg
      361 aacagaggat agctccccaa tagtctcctg attatccacg cgcgacattt gcaacatcgt
      421 atcacataat cttgttaacc ttaggctctc tgttttaaca agacctaata actcactttg
      481 tgtttcttca tctaattctg gatcttgtaa tagttctaca atattagcta ctgcagcaat
      541 cggagtcttt agctcatgag aaacattagt gataaagtct tttcgcatat gatccatatg
      601 agataaagaa acaatcatct gattaatact ttcagatagc tcatccaatt cattataata
      661 ctcataggat ttacgacgat tactgtttcg ataaatatgc ccttcaaagt caccgcctgc
      721 aacattggac acaatatggc taatatctaa aattggtttt gttaaatgat aagaaccgta
      781 ccacatacta atactagcta tcacaaggct taaaaaaaca acagcgagag cgctaacttg
      841 taatacatga gtagttaaga ctccttgaaa aaaagtaaaa actattgctg caactagtaa
      901 aagagaagct aggactgaga aaagcatcgt taacacaaac attaatgtga aataaaatct
      961 taacggaaac cgaaatcgcc ttagcttagt cattttttgc acgataccct acccctctca
     1021 ccgtttcaat cttaaaatct ggtaagtgtt ctacttttcg tctaatttta ttgatgcaag
     1081 catctaccac ccgctcatct aaatcagtat ccattcccca aatactatcc aatagttcta
     1141 aacgtgtgaa aatcctatta ggatagctaa gtaagtggaa aagaacccta aattctttct
     1201 gaggaaatac aatttcttgc tcaaaatatt tcaagcttaa ttcatcttca ttaagacaag
     1261 tattcccaat aattagctga tgttgagcta caatattagc tctcctcaag agtgccttaa
     1321 tacgtaaagc taattcttgt aactgcacag gcttaaccat ataatcatct gcaccaaggc
     1381 caaacccttt tgtcatatct tccatttgtg attttgctgt cataacaatg attgggacat
     1441 tgtaagcagc tgacctgagt tcttgaatta attgataacc atccatcatg ggcatcatga
     1501 tatcagttat aatcaattgt acttcttctg tatctagaca atttagagct tcttgtccat
     1561 ttgaagcagt gataaattgg tagccctctt gtgttaattt tgtggtaatg attttttgtt
     1621 gaacaatgtt atcctctaca actaatattt taatcattaa atcacctcta gcttattgta
     1681 acacaagtta gaagttttct ttaactaatt tcccgtcctt taaacgataa acggtatcta
     1741 ctaaatctag aacgcgctca tcgtgtgtca ccataatagc tgcagtatta tgtgatttaa
     1801 cttcctgacg aataagttct gtaacttgac gcccacgttc ttcatcaagg ctagctgttg
     1861 gctcatctgc caagataacc ttaggatgat taataaatgc tctagcaatt gctgcgcgtt
     1921 gcttttgtcc tcccgataat tggttagggt atttatgtgc acactgctct attcctaaat
     1981 ctgataataa tttattaatt tcttccaagc ttgttttctt atcatagtgt tttgcaaatc
     2041 gagcctcttg ttgcaactgt tcttggattg ttaaataagg aagcagttga tgactttgaa
     2101 atataaaacc tagtgcttca cggcgcaatt gtgttctttg tctttgagac aaatccgtaa
     2161 cgttctcatg attgacatac aaactacctt gatctgcacc aagaagtaac cctgcaatgg
     2221 ataaaagtgt gcttttacct gaacctgagg gtcccagtat agctacaaac tcacctgcag
     2281 aaactgataa atctaaatta tctaatattg ataaaagctc atctccatct ggatagtgtt
     2341 ttgagatgtg ttttaattct aagattgctg acattattct cctcccccat taataacttc
     2401 gactgggtcg attttagcaa cttttacaat tgacaaagca ctactaatca ttgcaatgac
     2461 taaaaataca aacgatacta gaataatatt ttgccaatta ataacaaatg gcatttgagc
     2521 tggtaataca taagagagag caatcgccaa tcgatctcca acaatgatac caaacagtgc
     2581 caatatgatg acttgactca attgaaatag ggcaatctct gacatttttg tcccaatagc
     2641 tttcatgacg ctaaattcat gacgtttttg aagtgtgatg atgtagaaaa agacacctaa
     2701 aataccagct gaagctacga caaggaccca taaaatcata ttcaaagtgg attgttcagc
     2761 actataacca ggaatatgtt gaatgacatc ttttttacta acagcttcta attgagttgg
     2821 taaatgagat atacttttat catttttcat aactaatgcc tgaggtaaga actggtattg
     2881 aggattaatc tttttattaa tttcggtata aatgtcttta tcaataaatg ccacaggtcc
     2941 gtgaccatac atactattat ggacaaatcc aacgactgtt agtgatatag atgatgactt
     3001 atcaatcact ttatcaccta actttatccc ttcagctttt aaactatcat ttaaaataat
     3061 agcttttttg taacttgtca gttgttttcc ctcagaaagt gtaggtgcca taaaagaatc
     3121 tttatcaata gcaaaataag aaatatcaat tttcttttca tgtccttgtc gagttaaact
     3181 cgaacgttga atatttaaag tggtactatc ttttaaattc aaactgttta aatctgtctg
     3241 atctttagtt gtcaatacag atgatgtaat cacttgttca gcaccttcat tcaaaatata
     3301 ggtttgagct gggttatttt caatagcagc actaacagct ctacctaaac cgtttgctaa
     3361 acctgataaa aaaactacca tgaacatcat aacaatgatt agcaattcaa tcaggaggta
     3421 ttttttctta taaaaggtta attctttcca agcatgtgac atggtttttc tcctttgatc
     3481 ttttgatgtg aacatagtat cacagcttta tgacttgaat atgacctcta aaaataatga
     3541 tattttatca ttaaattatt taaaaaatag cttgacaatt ttttagtaag cgattacaat
     3601 tctaattatg aaaccgttcc cacaataaaa gaaaggagtt tctatgagta cgattagaca
     3661 agtcgccgag aaagctggcg tatcaacatc tactgtctca aggtatatat cccaaaatgg
     3721 ttacgttagt caaaaagcaa gtcaaaaaat cgagcaagct atccgcgaac tccattatgt
     3781 tccaaatttc cttgcacaat ctctgaaaac caaaaaaaat caacttgttg gattactact
     3841 tcccgatatt tcaaaccctt tctttccaag acttgctaga ggagttgaag aatttctaaa
     3901 agaacagggt tatcgtgtca tgcttggcaa cacaaataac aagagccatt tagaagaaga
     3961 gtacttaaat gtgctattac aaagtaatgc tgcagggatt attacaacac acgatttcac
     4021 caaaaatcat cctgaaatag atattccagt tgtagttgtt gaccgcgtta atcaagaaac
     4081 acagtacggc gttttctctg ataataaaga gggaggaaaa ttagcagctc aagctatttg
     4141 gacagcaggt gctaccaata tattactcat tcgtggtccg ctagataaag ctgataacct
     4201 taatcaaagg tttcaaggaa gccaaaatta cctattaaac aaaggggctc gttttgctat
     4261 cgaagatagt gccagttttg attttgcaga gattcaaatt gaagcgaaaa ctttacttga
     4321 ccaccatcca gatattgata gcattatcgc tccatctgat atacatgcta ttgcctacct
     4381 acacgagatt cttaatcgag ggaagcgaat ccctgaagat gtacaaatta ttggttatga
     4441 tgatatttta atgagccaat ttatctaccc ttcgttgtca actattcacc aatcatcata
     4501 tattatggga caaaaagcag ctgaattaat tttcaaaatt actaatcaat tacctattac
     4561 caataagcgt attaaattac ccgttcatta tgtcgaacgt gaaaccctaa ggaggaaaat
     4621 agatgagtaa cattgtcatt atcggtagta tttccatgga cttagttatg gaaaccaatc
     4681 gtatcgccaa agagggagaa accgtatttg gccaacgttt ttcaatggtt ccaggaggta
     4741 aaggtgctaa tcaggctgtc gctattgggc gtcttagcca agatcgagat aatattacta
     4801 ttcttggtgc cattggcgag gattcttttg gtcccattct tttggataat ttaaacaaaa
     4861 accatgttac tacggatttt gtgggaacga taccatcttc ttcaggtgtt gcccaaatca
     4921 ctctttataa taatgataat cgaatcattt actgtcctgg agccaacgga aaagttgata
     4981 ctaaaaagtg gtctcaggag tggtcaataa ttaaagaagc tgatttagtt gtattacaaa
     5041 atgaaattcc tcatcaagca aatatgaaaa ttgctaattt ttgtaaagaa catagtatta
     5101 aattactcta taatcctgca ccatctagaa agactgatat tgagatgctc gataaggtcg
     5161 attatttcac accaaatgaa cacgaatgtc aagagctgtt cccaaaccaa aaactagagg
     5221 atatattagc cacatatcct gaaaaactaa ttgtaacact cggcacaaaa ggagccatct
     5281 actctgatgg aaaagaaagc catttaattc ctgccttaga aactaaagct gttgatacaa
     5341 ctggtgcagg tgatactttt aacggtgcat tcggttatgc tatctcgaaa aaatttaaga
     5401 ttgctaatgc cttacgtttt gcaactctag ctgctcactt atccgtgcaa aaatttggtg
     5461 cacagggtgg catgcctaca attaaagaaa tggaggacaa ccaacattat gaaaaagact
     5521 ggtatattaa atagtcatct cgctaaatta gcagatgacc ttgggcatac tgatcaagtt
     5581 tgtattggag atctgggtct acctgttcca aatggcattc caaagattga tttgtctttg
     5641 acctctggaa tacctagttt ccaagaggta cttgatatct atttagagaa tatcttagtt
     5701 gaaaaagtca ttttagcaga agaaatcaag gaagcaaatt cggatcaatt atcaaggtta
     5761 ttagctaaac ttgataactc tgttaacata gagtacgtca gtcacaatca tttaaaacaa
     5821 atgacccaag atgtcaaagc agttattcgt acaggtgaaa atacaccgta ttcaaatatt
     5881 attctacaat ctggtgttat catctagaaa ggagtttagt ccatgaaaat tgacatgagg
     5941 aatatctcca aatccttcgg tactaataaa gttctagaga agattgattt ggaattacaa
     6001 tctggtcaaa tacacgcact tatgggtgaa aatggtgcgg gtaaatcaac cttaatgaac
     6061 attctaacag gactttttcc agcaagtaca ggaactattt atattgatgg cgaggaacga
     6121 actttttcaa accctcaaga agcagaggaa tttggtatta gttttattca ccaagagatg
     6181 aatacttggc cggaaatgac tgtcttggaa aatctctttc ttggacgtga aatcaagaca
     6241 acttttggct tactcaatca aaaattaatg agacaaaaag cacttgaagc ttttaaaaga
     6301 ttaggtgtga ctattcccct agacatcccg attggtaact tgtctgttgg acaacaacaa
     6361 atgattgaaa tcgccaaaag tctcctaaac caactctcta ttttggtaat ggatgaaccg
     6421 actgctgctt taacagatcg tgaaactgaa aatcttttcc gagttatccg aggtttaaaa
     6481 caagaaggag tgggcgttgt ctatatctcc catcgaatgg aggaaatttt taaaatcact
     6541 gacttcgtaa ccgtcatgag agatggtgtc attgtagata caaaagaaac atcactcaca
     6601 aattctgacg aattggttaa aaaaatggtt ggccgaaaat tggaagatta ctatcctgaa
     6661 aaacattctg aaattgggcc tgttgctttt gaagttagta acctctgtgg agataatttc
     6721 gaggacgttt ccttttatgt ccgtaaaggc gagattcttg gattttctgg attaatggga
     6781 gctgggcgta ctgaagttat gcgaactatc tttggaattg ataaaaagaa gtctggtaag
     6841 gtgaaaattg ataaccaaga aatcactatt actagcccaa gccaagctat caagcagggc
     6901 attggttttt tgaccgaaaa tagaaaagat gaaggtctca tcctagactt caatatcaag
     6961 gacaatatga ctctccctag cactaaagat tttagcaaac atggtttctt tgatgaaaaa
     7021 acaagcacaa catttgtgca acaattgatt aatcgcctct acattaaatc agggagacca
     7081 gatttagagg ttggaaatct ttctggaggt aaccaacaaa aggtcgttct cgctaaatgg
     7141 attggtattg ctcctaaagt cttaattctt gatgagccaa cacgtggtgt tgatgtcgga
     7201 gctaaacgag agatttacca attaatgaat gaactagcag acagaggtgt tccaattgtc
     7261 atggtatcat cagacttacc tgaaattcta ggtgttagtg accgcatcat ggtaatgcac
     7321 gaaggtcgaa tcagtggaga actcagtcga aaagaagctg accaagaaaa agttatgcag
     7381 ctagccacag gaggtaagta aagtgaaaaa tatgatgaaa tatatatcag agttaacaac
     7441 cctgattgca ttagctggtt taatggttat tatcacactc attaatccta atttcctcac
     7501 aaccaataat ctactcaatt tattattaca agtaactgca aatggtttta tcgctttcgg
     7561 gatgaccttc gttattttga ctggcggtat tgatttatca gtcgggtcca tattagccct
     7621 atctagtgca ttaacagctg gattaatcgg ccacggagta cccgttggta ttgctatcgt
     7681 tttatctgtt gccttaggtg gcatcttggg aatgctaaac ggactcttca tttcttacgg
     7741 aaaattaaca ccatttatcg ttacactggc aacaatgact atctttagag gagcaacgct
     7801 agtttattca aacggcaacc caattaccgc tggattatcc gatagttttc tattccaatt
     7861 tttgggacaa ggctatattg ttggtatccc attcccagtc attctcatgt ttcttacttt
     7921 tattattttg tatattttgt tacataaaac agccttcggg aaatctgttt acgctttagg
     7981 aggtaatgaa aaagcagcgt atatctcggg tattaagtta aacaaagtta aaattatcat
     8041 ctacactatc tcaggtatca tggcttccat ttctgggtta attatcacat cacgccttag
     8101 ctcagcacaa ccaacagctg gagcaagtta tgagatggat gctattgccg ccgttgtctt
     8161 aggtggaacg tctttatctg gtgggaaagg acgtatcatt ggaacattga ttggtgctct
     8221 cattattggt gtcctgaaca atggattaaa catcattggt gtctctgcat tttggcaaca
     8281 agttgtgaaa ggtatcgtta ttttaatggc tgttttactt gatcgcttta aagtagcaaa
     8341 atagtcttta ctctaatttc tataagttca ttatatttta tctaaaggag tttcaaatga
     8401 aatttggaaa aaaacttggc tttctagcct tattgatgtc tattgtcctc attctcggtg
     8461 catgtggtaa aactggactt ggaaattctt ctgggaattc tacaaaaaat gtaaccaaaa
     8521 aatctgctaa agatttgaaa ctaggtgtat ctatttcaac aactaacaac ccctactttg
     8581 ttgcgatgaa agacggtatt gacaaatatg cgagcaataa aaaaataagc attaaggtag
     8641 ctgatgctca agatgacgcc gcacgtcaag cagatgatgt tcaaaacttt attagtcaaa
     8701 acgtagatgc tattttgatt aatcccgttg actcaaaagc tattgtaaca gctattaagt
     8761 ctgcaaacaa cgctaatatt cctgttattc taatggaccg tggtagtgag ggaggcaaag
     8821 tattaactac tgtcgcttca gataacgttg ctgctggtaa gatggctgcg gattatgccg
     8881 ttaaaaaact aggcaaaaaa gcaaaagcct ttgaattatc tggagtacca ggtgcctctg
     8941 ctactgtaga ccgtggtaaa ggatttcatt cagttgcaaa atctaaactt gacattcttt
     9001 caagccaatc tgccaatttt gaccgcgcca aagctttgaa tactacacaa aatatgattc
     9061 aaggacataa agatgtccaa attattttcg cacaaaatga tgaaatggca cttggggctg
     9121 cacaggctgt caaatctgct ggcttgcaaa atgttcttat cgtaggaatt gatggtcagc
     9181 cagatgccca tgatgccatt aaaaaaggtg atatttcagc cactattgca caacaaccag
     9241 ctaagatggg agaaatagct attcaagcag caattgatca ttataaaggc aaaaaagtag
     9301 agaaagaaac gatttctcca atttaccttg ttaccaaaga caatgttgaa aaatacaatt
     9361 ggtaaaagta cacaaaaagt cgtgaaaatt cacgactttt tatattataa ttttgctaac
     9421 atattatcga tatgttgaac tgatttttct ttacctaata aataaatagt atctggtaac
     9481 tctggtccat gcatttcacc agaaaccgca atacgaatag gcataaatag gttcttacct
     9541 ttgatgcctg tttctttttg aacagcctta atttgaggga aaatagtatc tctagtgaat
     9601 tcttcgtcag aaagcgatac taacttttcc ttaaaagcag ataagactgt tgggactgtt
     9661 tctgctgcca ttacttcttt ttcggcttct gttaattctg gaaaatctgc aaagaaaagg
     9721 tctgttaatg gtacaatctc atcagccgat tttaattgag gtttataaag ctcaactaat
     9781 ttttcagctt tgtctgtaag tcgaccagct tcttctaaga atggtttgca aagtgcaaaa
     9841 acactctcaa aatcagcatt ttttaggtaa tcattactca tccaatccat cttcttctga
     9901 tcgaatgctg ctggtgattt gctaagacga ttttcatcaa atagattaat taattgctcg
     9961 cgtgagaaga tttcttcttc tccaccagga ttccaaccaa gaagggcaat gaaattaaag
    10021 acagcttccg acatataacc ttttttacga tagtcttcga taaattgaag agtatttgtg
    10081 tcgcgttttg ataatttctt accagtctct gagtttataa tcaatgtcat gtgaccaaat
    10141 tgtggcgctt cccaaccgag tgcttcataa accattaatt gttttggcgt atttgcaata
    10201 tggtcatcgc cacgaataac atgcgaaatt tgcatgtcat gatcatcaat gactactgcg
    10261 aaattgtaag ttggataacc atctttcttc tgaataaccc agtctccgcc gatattagag
    10321 ccttcaaact caatgtcacc ttttaccata tcagtccact tataaatccc agattcatta
    10381 acagcaaggc gtactgtcgg aatgatgcct gctgcttcac gttctgcaat gtaagcttct
    10441 ttttcagttt ctgacattcc gataaattcg ttgatatagc gaggtgtttc gcctgccaat
    10501 tcttggcgtt cacgctctgc tgctaattct tcctcagtca cataggactt gtaggcttta
    10561 ccttctgcta acaactgatc aatataacgt tggtagagct caaggcgttc tgattgacga
    10621 taattttcat gtgtttcagg gctttcatcc cagtccatac ctagccaacg taagttctcc
    10681 aattgagagc gttcaccatc ttcgacgtga cgttttcggt cagtatcttc aatacgaata
    10741 acaaaatccc caccatgatg gcgagcataa aggtagttaa agagcgcagt acgcgcattt
    10801 ccgatgtgta acaatcctgt tggacttggt gcataacgca cacgaatctt attagccaaa
    10861 attctactcc tatattttct gatagtaaac tttttcagtt taactgaatt agtcaagggg
    10921 gcaaaacatc attcaaactc ttacagatga ttgccaatca accctattat accactaaat
    10981 cactaaaaat gataatcagt aataatagtc acactaattt tcgataaa
//
LOCUS       DATRSG010000066         9871 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRSG010000066 DATRSG010000000
VERSION     DATRSG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.066"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            305..1474
                     /locus_tag="VBD89_001696"
     CDS             305..1474
                     /locus_tag="VBD89_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7136206.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1471..1617
                     /gene="rpmG"
                     /locus_tag="VBD89_001697"
     CDS             1471..1617
                     /gene="rpmG"
                     /locus_tag="VBD89_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7136207.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1664..1900
                     /gene="secG"
                     /locus_tag="VBD89_001698"
     CDS             1664..1900
                     /gene="secG"
                     /locus_tag="VBD89_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO7136208.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2013..4418
                     /gene="rnr"
                     /locus_tag="VBD89_001699"
     CDS             2013..4418
                     /gene="rnr"
                     /locus_tag="VBD89_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO7136209.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4421..4888
                     /gene="smpB"
                     /locus_tag="VBD89_001700"
     CDS             4421..4888
                     /gene="smpB"
                     /locus_tag="VBD89_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO7136210.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4972..6360)
                     /locus_tag="VBD89_001701"
     CDS             complement(4972..6360)
                     /locus_tag="VBD89_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7136211.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6489..7415)
                     /locus_tag="VBD89_001702"
     CDS             complement(6489..7415)
                     /locus_tag="VBD89_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7136212.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7544..7936)
                     /gene="gloA"
                     /locus_tag="VBD89_001703"
     CDS             complement(7544..7936)
                     /gene="gloA"
                     /locus_tag="VBD89_001703"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO7136213.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8024..8698)
                     /locus_tag="VBD89_001704"
     CDS             complement(8024..8698)
                     /locus_tag="VBD89_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7136214.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8965..9807
                     /locus_tag="VBD89_001705"
     CDS             8965..9807
                     /locus_tag="VBD89_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7136215.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
BASE COUNT         3326 a         1600 c         1908 g         3037 t
ORIGIN      
        1 aacgatgcca atattattag taagcctagg aattatagtt gttattttaa ttattggcta
       61 tttagcagtt gtagtatttg ctccaaaaac tttccgttta aaaagttaat tttaattgtg
      121 gtataataac agaggcatgt gataacatag gtttagttac ataaaggaaa ttataatgga
      181 aataacatct caaatagatg ttaaatctca ataaaactgc taagatagtt agtggtagaa
      241 aagtttagaa agaagatgat gttttatctc agttgaaacg gttaactgga agcaaaattt
      301 aaaagtggct tggctcggta atttttttac tggggcaagc ttttcacttg ttatgccttt
      361 tatggtatta tatgtagaac aattaggagc acctagcaac aaagtagagt ggtatgcagg
      421 cctatcagta tccttatcag ccttgtcttc agctttagtt gccccattat gggggcgctt
      481 agctgataaa tatggtagaa aaccgatgat ggtaagagca gggttgatga tgacttttac
      541 tatgggaggc cttgctttca ttcatagtgt tacaggactt ctgattttac gtatattaaa
      601 cggcattttt gcaggctatg tacctaactc tacagctttg atagcaagtc aagctcctca
      661 agaggaatca ggatatgcct taggaacctt agcgacagga gtgactggag gtatgttaat
      721 tggtccactt ttagggggac ttttagcaga gtggtttggt attagggaag tttttttatt
      781 agtgggaacc atattgttaa tatctacttt aatgaccatt tttatggtaa aggaagattt
      841 caaacccatt tctaacgaag aaaccatgcc aactacagaa gtgttcaaaa gcgttaaaag
      901 cttacaaatt ttaatagggc tgtttgtaac aagcatgatt attcaaattt ctgcacaatc
      961 aattgcacct attttaacac tttacattcg tcatttagga cagactgaaa acttgatgtt
     1021 tgtttccgga ttaattgttt ctggtatggg attctctagt attctgagta gtccaaagct
     1081 aggaagaatc ggggatcgca taggcaatca tcgtttgcta ttattagctc tactgtatag
     1141 tttcctcatg tatgtcttat gttcattggc acagacttca ttacagctag gggttattag
     1201 attcctgtat ggttttggaa caggtgcctt gatgccgagt attaattcaa tcttgactaa
     1261 aattgctcca agacaaggtt tatcaaggat tttcagttat aaccaaatgt ttagcaattt
     1321 aggacaagta ttaggtcctt ttgtcggttc agcagtttct attcatttag gattccgatg
     1381 ggttttcttt gtgactagct tcatcgtttt agctaatttt gtttggtgtt ttattaattt
     1441 tagaaaatat attagagtta aggaaattgt gtgagagtta aaattaattt aaagtgtagc
     1501 tcttgtggta gtaagaatta tttaaccagt aagaataaaa gtaatcatcc cgataaaatc
     1561 gaggttccaa agtattgccc aaaggagaga aaagtaacac tacatattga atcttaataa
     1621 gttttatgtt agaatgtact ttgaccatag tggaggaatt aacatgtata acctattact
     1681 caccattcta cttgttttat cagtattatt gattatctca attttcatgc aaccacaaaa
     1741 gaatccaagt agtaatgttt ttgatagtag cggttctgaa gccctttttg aacgtagtaa
     1801 agctcgagga tttgaagctt ttatgcaacg ttttacaggt gttttagtct ttttctggct
     1861 actaatcggc ttagtattat ctattttatc aagtcattaa tgatagctaa atacagataa
     1921 gtgaggatgg gggatagccc aacctcactt tttgttagta gaatggttgt agagtaataa
     1981 taaagtgact aaagttacta gaaaaggaaa taatgaaaga aaaaattata aaatatttgg
     2041 aagaacacgg taaaaccagc gttaatgatt tagctgctgc cctagatatg gcaggcgcaa
     2101 aagcatttcc aaaattaatc aaaacaatct caaatttgga gagccacagg caattaagat
     2161 ttgacgataa tggaagtctt tctcttcaaa agaaagaagc taagaaaaag gaaatcacag
     2221 taaggggtct ttttagagca aataaagcag gatttggttt tcttagtatt gaccaagacg
     2281 aagatgatat gtttatcgga aaaaacgata ttgcttatgc tattgatggt gacacggtag
     2341 aagctgttgt aaagaaacca gcggaccgtc ttaacgggac tgcagcagaa gcgcgtgttg
     2401 ttaatattgt tgaacgtagt ttaaagacgc ttgttggtaa gtttgtctta gacgatgagc
     2461 gccctaaata cgcaggatat atcaaatcga aaaatcaaaa aatcaatcaa aagatttata
     2521 tacgcaaaga accggtcgtt ttagacggaa cagaaattat caaagtagat attgataaat
     2581 atccgacacg aggacatgat tacttcgtag caagtgttcg tgatatcgtt ggacatcaag
     2641 gagatgttgg tattgatgtt ttagaagtct tggaaagtat ggatatcgtc tcagaatttc
     2701 ctgaggacgt tattgcagaa gccaatgcta tcccagatgc tccaacggag aaagatttaa
     2761 ttgggcgtgt tgatttacgt caagaggtca ctttcactat tgatggagcg gacgccaaag
     2821 atttagacga tgctgtgcat attaaattac tggataatgg tcattttgaa cttggagttc
     2881 atatcgcaga tgtatcatac tacgttactg aaggctctgc cttaaatcgt gaagcactta
     2941 gtcgtggtac gtccgtatat gtcacagatc gcgttgtacc aatgttacca gagcgtctat
     3001 ctaacggtat atgttcatta aatccaaatc ttgatcgttt aacgcagtct tgtattatgg
     3061 aaattgacca aaatgggaga gttgttaatc atcaaattac acagtcagtg attaatacca
     3121 cttatcgtat gacttatatc gctgtaaatg atattattgc tggcgatgag gaaatctgct
     3181 cagaatatga gtctattgtg tcctcagtac agcatatggt cactttacac catactttgg
     3241 aagctatgcg tacgcgtcgt ggagcgctga attttgacac atctgaagca aaaattatgg
     3301 ttaatgataa gggaatgcca gttgatattg ttattcgcaa cagaggcatt gctgaacgca
     3361 tgattgagtc atttatgttg gcggcaaatg aaacggttgc tgaacattat gctcgtctaa
     3421 aactgccatt tatttatcgt atacacgaag agcctaaagc tgaaaaatta caaaaattca
     3481 tcgattatgc ttctgttttc ggtgttcaga ttcaaggaac agcaactaaa atcacccaat
     3541 ctgctttaca ggactttatg aaaaaagtac aagggcagcc aggaagtgaa gtgttatcta
     3601 tgatgttatt gcgctcgatg caacaagcta gatattctga acacaaccat ggacattatg
     3661 gacttgctgc ggaatattac actcacttta ctagccccat tcgacgttat ccagacttgt
     3721 tagttcatcg tatgattcgt gattatgatg ataaagcgat ggataaagct gatcattttg
     3781 ctaaccttat cccagaaatt gcaactcaaa cgtctagtct tgagagacgt gccattgatg
     3841 ctgaacgtat tgtagaggca atgaagaaag ccgaatatat ggaagaatat gttggtgagg
     3901 aatttgaagg tgtggttgca agcgttgtta aatttggtat gtttgtagaa ttaccaaata
     3961 ctattgaagg attgattcac gtaacgacct tgcctgaata ttatcacttt aatgaacgta
     4021 cgctgacgct tcaaggtgag aaatctggga aagttttccg tgttggtcaa caaattaagg
     4081 ttaagttgat tcgttctgat aaggaaactg gtgatatcga ttttgattat ttaccatcag
     4141 attttgatat tgttgaaaag gtaagtaaaa gctcgcgtga gggtcgtcct aatagaagtt
     4201 ctaaacgaga acatcagcat agaatttctg atcgtgataa taagaataaa aatacatcta
     4261 agaaaaaagc atctcgaaaa cccaaaagga actcggatag caaatcacat catcataaag
     4321 acgatagaac aacaggttca accaagaaaa aaacgaaaaa acctttctat aaaggcgttg
     4381 ctaagaaagg tcagaaacgg aaatctaaga aaggttaagt atggttaaag gtcaaggaaa
     4441 cgttgtagca caaaataaaa aagcgcatca cgactatact atcgttgaaa caatagaagc
     4501 aggaattgtt ttgacaggaa cagaaatcaa aagtgtccgc gcagcgcgta ttacattgaa
     4561 agatggatat gcacaaataa aaaatggtga agcgtggttg attaatgttc atattacacc
     4621 atatgatcaa ggtaacatat ggaatcaaga tcctgatcgt acacgaaagt tattactaaa
     4681 aaaacgtgag attgaaaaaa tatcaaatga actgaaaggg actggtatga ctctggtacc
     4741 tctaaaagtt tacttaaaag atggttttgc taaagttcta ttgggtttgg caaaaggaaa
     4801 acatgattat gataagcgag agtctatcaa acgtcgtgag caaaaccgtg atattgcccg
     4861 tcagttaaaa aactataatt cacgatgatt atcaaaaggt tagggaattg cctaaccttt
     4921 tttggacgaa aaaagtgaag ttgattgacc aacttcacta ataatataga cttaatgtga
     4981 tttatgtttg aagtgactat ataaaccaaa tccaataatc cagatggttg ctccaatagc
     5041 cccgatgtat gttgaatctt gcaagaagag agaaacgaat acaaagagga agaagagaag
     5101 tgtgattggt gaaaggattt tgtaggcagg cattttataa ccatcttcca taaaatcagg
     5161 tgatttacga tatttccaat gtgcaatcat aataagaaca tagatagaaa tgtaaacacc
     5221 agaagatgat gcagtaatta aggagaaggc atctgaaata ccaggtaaaa cactgataag
     5281 tgctgaagct ccaacgatta cagctgatgc aataatagct cgacttggaa cgctttgacg
     5341 agataattgg tctaatttta acgcttttgt aagagcattt ggtgattcat tagccaattg
     5401 gaataaatga cgccctgttg agtacaaagt agaattaaga gcagatgctg cagacgttag
     5461 gacaacaaag ttaatcaaag cggcagccca cttaatccca gctagtttga atactgtaac
     5521 gaaaggtgac tcattaactg gtaactgttg ccatgggaag atggccataa ttgctaataa
     5581 tgaacctgcg tagaataaaa taatacgcat cggaatttct tggattgctt ttggtaaaac
     5641 tttacgtgga ttagctgttt cagaagttgt tactccgaca aattcaatag ctaaataagc
     5701 aaagaatacc atttggaaag ccatgaagaa attcaattta cctttaggga accattcaaa
     5761 atgattggtg atatttgaga ttgaagcatg gtatccgtga ccggtgtcaa agtttgtcaa
     5821 taccataaag ataccggttg ctattaaacc aagaattgca ataactttta tcatagcaaa
     5881 ccaaaactcg gtttctccga atgcttttac agcaattaag ttgatagaac ttaggatagc
     5941 taaaaaaaca atttggataa tccaggatgg ccattcaggg aaccaaaatt gaacatactc
     6001 tcctacggct gtaatctctg ccataccgat aaatattagg gaaatccaat aagaaagtgt
     6061 tgagaaaaat ccccaaccgg gtcctaaata tttggtaatg aaattaataa aagtatgctg
     6121 atttggatct tgataaagca tctctccaat agcacgcatc atgaagaaca tgaaagcgcc
     6181 agtgatagca tagacaagaa ctattgaagg acccgtcagg ctaattgaac gacctgcacc
     6241 aagaaaaagc ccagttccga ttgtccctgc aatcgcaatt aactgtacgt gacgattttc
     6301 taggccgcgt accataccat tctcagattt ttgagtgtgg tcattattat tttttgacat
     6361 tataaatact ccatctattt ttgaaaaact ccttcattat ataatttttt tgcaaaattc
     6421 gcaataatta ctatacaata tttttctatg ataaaatctc ctagctaagt gagctaagag
     6481 atttcaattc atccctcttc cttgacaata tagactgggc gttttttagt ttctaggaaa
     6541 atcttagaga tgtatttgcc tataattcct agcgacaata actgaatacc acccataaaa
     6601 agtacaatgg taactgtgct tgcccaacct gaaacgggat ccccaaatag taattttcga
     6661 attataataa aaatgatagc taaaagtgat agtactgaac taagggttcc aatccaggta
     6721 gctatagcta atggaatttc tgagaagttg atgaaaccgt ctagagagta ttttaagaga
     6781 ttaaagaaac tccatgaggt cttacccgca attcgttctc gattctcata actaatataa
     6841 gcgacatcat atccaatcca ggaaaaaatc ccttttgaga agcgattcac ttcatttagc
     6901 tcgagaatac tgtcaacaac ttgttttgtc atgagacgaa aatcacgcgc accatcaacc
     6961 attttagtat ctgacacttg attgattaat ttataaaaaa gcttggcaaa aagagagcgt
     7021 atcaacggct cccctttacg atcttttcgc cttgtagcga caatatcata accttcttga
     7081 agttttaggt acatttctgg taataaagtg ggtggatctt gcaagtcaac atccataaca
     7141 gtgataaaat caccagtagt atgttctaaa ccagccaata aagcagcatc tttaccaaaa
     7201 tggcgtgaaa atgaaaggta atgaacattg tcaaattgtt tagctgcttt cttaagaagt
     7261 tcgagtgtac gatcggtaga accatcgttt acgaaaatat attcaaaatg ggtgtaacga
     7321 gccatttcgg attcaacttt tttgatttct gttaaaaaag ttgagacagt ctcttgttca
     7381 ttataacaag ggacaataac tgataataaa gccatttgta attctccgta tcattcttgc
     7441 ttaaatttta acataatatg ggaaaaaata gaaagattat aaggtaatca taaaaaggac
     7501 taggtttttg cctcagtcct taacttaata tgatgagatg ttattagtca gctttaaatt
     7561 gactgagtcg aataacttca attttatagc catctggatc ttgaataaag tagaacatat
     7621 taggtttacc tggtaatcca gaaatttttg ttacagagta acctgctttc tgatgggcat
     7681 cataagtagt ttccaaatcg tctacaccaa cagcaatgtg accataccca tttcctaaat
     7741 cataagcttc gtgatcatag ttataggtta actctaattc gtaactaggg tcgtcctcta
     7801 gggacatata gactaaagta aattgatttt caggaaagtc attacgacga acttccttaa
     7861 aaccaagtgc ttcttgataa aaagcaattg aggcgtccaa atctttaacg cgaatacaag
     7921 tatgtaaaaa tggcatagtc tctctcctat ttctttatta aacgtattat gtcaaaataa
     7981 aattatcttt tcaaaaatat tgattataaa ttaggctgtc tatttactca acccaagtaa
     8041 tgacctcttg acgttctttc ctagcacggg aatgtttagg ttcacgcaaa cgataaccaa
     8101 atgaaaccat acaagaaata gcttctttct tatcgtctat taacccttct ttactaagaa
     8161 tattattgac cttttcatag tcaaatcctt caattgggca tgaatctacg ccgatcatag
     8221 cagcagccgt catcatatta ccaagtgcga tataagtttg ttttgctgtc caatcccaaa
     8281 gagaacgttc agaatcaatt ttcatatcgt tttcttggaa ggatttgtag agaggtatcc
     8341 tactctcaag ggcggctgga tcacctagac cacgtcgaat gagactgtta atcatgctat
     8401 cagcgttata ataagcacct ttttcagcta gtagtaaaac aaagtgacta gcagtatcta
     8461 gttgatattg tgctccccaa gcaacttcct taagtttatc tcgaaatttt gcaattgttt
     8521 gtctatctag tactaaaaat cgccagcctt ctaaaccgac tgaagaagga ctcaaccaag
     8581 cagtgtctaa aatatactcc atatcttctt taggaatatc attattatta taaacacgaa
     8641 tcgccatacg aaaatcaaaa gctcttcgaa cttgtttttt gatatcttct tttgtcattt
     8701 tagatcctcc tgttattaat gctatcatat cacaatttag aaaaaatctc agcaggcggt
     8761 atcgtggcaa agtagagcta caaaattcta atgactgtca gaaaaataaa gggaatgaac
     8821 aaaatgatag ggttttgatg aagaaagtta tataatttta cataaggagg ggataatacc
     8881 atatatcaaa ctatcattta gatgtttaaa atagatgttt ttcactatga aattcatttt
     8941 tgtcacatag aaaaggagat tgttatggaa acatatactc tatcgaatac tcttaacatt
     9001 cctaaaattg gttttgggac atggcaacta actgaaggtg aagaagctta taaagcagtg
     9061 actcatgctt taaaagttgg ttatactcat attgatactg ctcagattta tggtaatgaa
     9121 catagtgttg gacgtgctat aagagacagt ggtttggcgc gagaaagtat ctttttgaca
     9181 actaagattt ggaatgacaa acatgactat catttagcca aagcttctat tgatgaatct
     9241 ctgcagaaat taggtgttga ttatattgat cttcttttga ttcattggcc aaatccaaaa
     9301 gctcttcgtg aaaatgatgc gtggaaagct gggaatgcag gcacttggaa agcgatggaa
     9361 gaagcttata aagaaggaaa ggtaaaagct attggtgttt cgaatttcat gaaacatcac
     9421 ttagaggctt tatttgaaac cgccgaaata aaacctatgg ttaatcaaat tatcctagca
     9481 ccaggttgtg ctcaagaaga tttggttcgc ttctgtaaag gaaatgatat cttattagaa
     9541 gcttacagtc catttggaac aggtactatt tttgaaaatg aatctataaa agctattgct
     9601 gaaaaatatg gtaaatcagt tgcacaagta gctcttcgct ggtcacttga taatggcttt
     9661 ttaccattac ctaagtcagc gactcccaaa aatattgaag caaaccttga tatttttgat
     9721 tttcaattaa acgaagatga tattgctacc ttaatacaac tagattcagg tattaaaccc
     9781 aaagatccag ataatgtctc attttaataa gaaaagatgg aaatgtattt ccatcttttt
     9841 tatgtgggat agttccttaa agttaagaaa a
//
LOCUS       DATRSG010000067         9783 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRSG010000067 DATRSG010000000
VERSION     DATRSG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.067"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..93
                     /locus_tag="VBD89_001706"
     CDS             <1..93
                     /locus_tag="VBD89_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO7136216.1"
                     /translation="LIYETITIILLIIGRKHLKLRKYKNKMQAL"
     gene            373..4485
                     /gene="cas9"
                     /locus_tag="VBD89_001707"
     CDS             373..4485
                     /gene="cas9"
                     /locus_tag="VBD89_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO7136217.1"
                     /translation="MNKPYSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEDDKRGSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAH
                     IIKFRGHFLIEDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIP
                     YYVGPLAREKSDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLISDGRANRNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIVPQAFIKDDS
                     IDNRVLVSSAKNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QVNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKAQKLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQ
                     HVSYFDDILQIINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSL
                     GAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
     gene            4487..5356
                     /gene="cas1"
                     /locus_tag="VBD89_001708"
     CDS             4487..5356
                     /gene="cas1"
                     /locus_tag="VBD89_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO7136218.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLVFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARYDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDTSNREGHAARIYFNTL
                     FGNDFSREHDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYDGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            5353..5694
                     /gene="cas2"
                     /locus_tag="VBD89_001709"
     CDS             5353..5694
                     /gene="cas2"
                     /locus_tag="VBD89_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO7136219.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            5681..6346
                     /gene="csn2"
                     /locus_tag="VBD89_001710"
     CDS             5681..6346
                     /gene="csn2"
                     /locus_tag="VBD89_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO7136220.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     repeat_region   6447..6746
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6447..6482
                     /rpt_unit_seq="gttttagagctgtgctgtttcgaatggttccaaaac"
     gene            7025..7162
                     /locus_tag="VBD89_001711"
     CDS             7025..7162
                     /locus_tag="VBD89_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136221.1"
                     /translation="MVKGLDCSTLYLIISLTALVLLVIAGVSLSLILASLQGKQWRTY
                     P"
     gene            7303..7719
                     /gene="ndk"
                     /locus_tag="VBD89_001712"
     CDS             7303..7719
                     /gene="ndk"
                     /locus_tag="VBD89_001712"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO7136222.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            7855..9687
                     /gene="lepA"
                     /locus_tag="VBD89_001713"
     CDS             7855..9687
                     /gene="lepA"
                     /locus_tag="VBD89_001713"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO7136223.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
BASE COUNT         3294 a         1482 c         1926 g         3081 t
ORIGIN      
        1 ttaatctatg aaacaataac tataattctt ttgataatag gtagaaaaca tttaaaatta
       61 agaaaatata aaaacaaaat gcaagcctta taatagcgct tgcattttgt ttgctatttt
      121 tttataatac aaatatgaga aatgtcggaa ttataacaat gacataaaaa aagcaccgaa
      181 tcggtgctca ctttttcaag ctaaatacgg actggattaa aaaatcactg ccttatttta
      241 acgtgctgtg ctgtttcgaa taattccaac aaaattatta tatgttattt taatgtaata
      301 atcaagtaaa aattagcaaa actttaaagt ttgcgttttt aaagtgatat tttaaataaa
      361 aggagataaa ttatgaacaa accatattca ataggtctcg acatcggaac taattccgtc
      421 ggatattctg tggttacaga tgattataag gtacctgcta agaagatgag ggtcttaggg
      481 aatacagata aagaatacat aaagaagaat cttataggtg ctctgctttt tgatggtgga
      541 aatactgctt cagatagacg cttgaagcga actgctcgcc gtcgttatac acgtcgtaga
      601 aatcgtattc tatatttaca agaaattttt gcagaggaaa tgagtaaagt tgatgatagt
      661 ttctttcatc gattagagga ttcttttcta gttgaggacg acaagagagg gagcaagtat
      721 cctatctttg caacaatgca ggaggagaaa gattatcatg aaaagttccc aacgatttat
      781 cacttgagaa aagaattagc tgacaagaaa gaaaaagcag accttcgtct tgtttatctg
      841 gctctagctc atatcattaa attcagagga catttcctaa ttgaggatga tagctttgat
      901 gtgaggaata ctgatattca aagacaatat caagcttttt tagaaatctt tgatactacc
      961 ttcgaaaata accatttgtt atctcaaaac atagatgtag agggaattct aacagataag
     1021 attagcaagt ctgcgaagaa agatcgcatt ttagcgcagt atcctaacca aaaatctact
     1081 ggcatttttg cagaattttt gaaattgatt gtcggaaatc aagctgactt caagaaacat
     1141 ttcaatttgg aagataaaac gccgcttcaa tttgctaagg atagctacga tgaagattta
     1201 gaaaatcttc ttgggcagat tggtgatgaa tttgctgact tattctcagt agcgaaaaag
     1261 ctatatgata gtgttctttt atctggcatt cttacagtaa ctgatctcag taccaaggcg
     1321 ccactttctg cctctatgat tcagcgttac gatgaacata gagaggactt gaaacagtta
     1381 aaacaattcg taaaagcttc attgcctgag aaatatcaag aaatatttac tgattcatca
     1441 aaagatggct acgctggtta tattgaaggc aaaactaatc aaggagcatt ttataaatac
     1501 ttgtcaaaat tgttgaccaa gcaagaaggt agcgagtatt ttcttgaaaa aatcaagaat
     1561 gaagattttt tgagaaaaca aaggaccttt gataatggct caattccaca ccaagtccat
     1621 ttgacagaat tgaaggctat tattcgacgt caatcagaat actatccatt cttgaaagag
     1681 aatctagata ggattgaaaa aatccttacc tttagaattc cttattatgt cgggccacta
     1741 gcacgtgaga agagtgattt tgcatggatg actcgcaaaa cagatgacag tattcgacct
     1801 tggaattttg aagaattggt tgataaggaa gcatctgcgg aagcttttat ccatcgtatg
     1861 accaacaatg acctctatct tcctgaagaa aaagtcctac caaaacatag tcttatttat
     1921 gaaaaattta cggtctataa tgagttgact aaggttagat ataaaaatga gcaaggtgag
     1981 acttattttt ttgatagcaa tattaaacaa gaaatctttg atggagtatt caaggaacat
     2041 cgtaaggtat ccaagaagaa gttgctagat tttctggcta aagaatatga ggagtttagg
     2101 atagtagatg ttattggtct agataaagaa aataaagctt tcaacgcctc attgggaact
     2161 taccacgatc tcaaaaaaat actagacaaa gattttcttg ataatccaga taatgagtct
     2221 attttggaag atatcgtcca aactctaacc ttatttgaag atagagaaat gattaagaag
     2281 cgtcttgaaa actataaaga tctttttaca gagtcacaac taaaaaaact ctatcgccgt
     2341 cactatactg gctggggacg attgtctgct aagttaatta atggcattag agataaagag
     2401 agtcaaaaaa caatcttgga ctatctgatt tctgatggaa gagcaaatcg caactttatg
     2461 cagttgatac atgatgatgg tctatcattt aaaccaatta ttgacaaggc acaggctggt
     2521 agtcattcag ataatctaaa agaagttgta ggtgagcttg caggtagccc tgctattaaa
     2581 aaggggattc tacaaagttt gaaaattgtt gatgagcttg ttaaagtcat gggatacgaa
     2641 cctgaacaag ttgtggttga gatggcgcgt gagaatcaaa caacaaatca aggtcgtcgt
     2701 aacactcgac aacgctataa acttcttgag gaaggcgtta agaatctagc tagtgacttg
     2761 aatggcaata ttttgaaaga atatcctacg gataatcaag cgttgcaaaa tgaaagactt
     2821 ttcctttatt acttacaaaa tggaagagat atgtacacag gggaagcact agatattgac
     2881 aatttaagtc aatatgatat tgatcacatt gttcctcaag ctttcataaa agatgattct
     2941 attgataatc gtgttttggt atcatctgct aaaaaccgtg gaaaatcaga taatgttcct
     3001 agcattgata ttgtaaaagc acgcaaagca ttctggaaaa aattacttga tgctaagtta
     3061 atgagtcagc gtaagtatga taatttgact aaggcagagc gcggaggcct aacttccgat
     3121 gataaggcaa gatttatcca acgtcagttg gttgagacac gacaaattac caagcatgtt
     3181 gcccgtatct tggatgaacg ctttaataat gagcttgata gtaaaggtag aaggattcgc
     3241 aaagttaaaa ttgtaacctt gaagtcaaat ttggtttcaa atttccgaaa agaatttgga
     3301 ttctataaaa ttcgtgaagt taatgattat caccatgcac atgatgccta tcttaatgca
     3361 gtagttgcta aagctattct aaccaaatat cctcagttag agccagaatt tgtctacggt
     3421 gactatccaa aatataacag ttacaaaacg cgtaaatccg ctacagaaaa gctatttttc
     3481 tattcaaata ttatgaactt ctttaaaact aaggtaactt tggcggatgg aaccgttgtt
     3541 gtaaaagatg atattgaagt taataatgat acgggtgaaa ttgtttggga taagaagaaa
     3601 cactttgcga cagttagaaa agtcttgtca taccctcagg tcaatatcgt gaagaagaca
     3661 gagattcaga caggtggttt ctctaaggaa tcaatcttgg cgcatggtaa ctcagataag
     3721 ttgattccaa gaaaaacgaa ggatatttat ttagatccta agaaatatgg aggttttgat
     3781 agtccgatag tagcttactc tgttttagtt gtagctgata tcaaaaaagg taaagcacaa
     3841 aaactaaaaa cagttacgga acttttagga attaccatca tggagagatt cagatttgag
     3901 aaaaatccat cagctttcct tgaatcaaaa ggttatttaa atattaggga cgataaatta
     3961 atgattttac cgaagtatag tctgttcgaa ttagaaaatg ggcgtcgtcg attacttgct
     4021 agtgctggtg aattacaaaa aggtaacgag ctagccttac caacacaata tatgaagttc
     4081 ttataccttg caagtcgtta caatgagcta aaaggaaaac cagaagaaat tgaacaaaaa
     4141 caggaatttg tagttcaaca tgtttcttat ttcgacgata ttcttcaaat aatcaacgat
     4201 ttttcaaatc gagttattct agcagatgct aatttagaga aaatcaataa gctttaccaa
     4261 gataataagg aaaatatatc agtagatgaa cttgctaata atattatcaa tctatttact
     4321 tttaccagtc taggagctcc agcagctttt aaattttttg ataaaatagt tgatagaaaa
     4381 cgctatacat caactaaaga agtacttaat tctaccctaa ttcatcaatc tattactgga
     4441 ctttatgaaa cacgtattga tttgggtaag ttaggagaag attgatatgg caggttggcg
     4501 aaccgttgtt gtaaatacac attctaagct ctcttataaa aataatcatc tggtttttaa
     4561 agattcttat cagacggaaa tgattcatct atcagagatt gacattctaa tcatggaaac
     4621 aacagatatc gttttgtcga ccatgctgat taaacgtttg gttgatgaaa atattttagt
     4681 tatattttgt gatgacaaac gcttgccaac agctatgtta atgccgtact atgccagata
     4741 tgattcgagt ttacaattat ctaggcagat gtcatggatt gaagatgtca aagcggatgt
     4801 ttggacgtca atcattgcac aaaaaatttt gaatcagtct ttttatcttg gtgagtgttc
     4861 tttctttgaa aaatcccagt ctattatgaa tctctatcat gatttagaat cttttgatac
     4921 ttccaatcgt gaagggcatg cagctaggat ttatttcaat acactttttg gaaatgattt
     4981 ttcaagagaa catgataatc caataaatgc tggtttagac tacggatatt cattgctttt
     5041 gagtatgttt gcgcgtgaag tcgttaagtg tggttgcatg acacaatttg ggttaaagca
     5101 tgctaatcaa tttaatcagt tcaacctagc aagcgatatt atggaaccat ttcgcccaat
     5161 cgttgatagg attatttatg aaaataggca gagtgatttt gtcaaaatga aaagagaact
     5221 cttttctatg ttttcagaga catacagtta cgacggtaaa gaaatgtatc tttcaaatat
     5281 tgtcagcgat tacaccaaaa aagttattaa gtcgctaaat agtgatggga atgggattcc
     5341 ggagtttagg atatgagtta tcggtatatg agaatgattt taatgtttga tatgcctact
     5401 gaaacagtag aagaacgtaa ggcgtatcgt aagtttagaa agtttctttt gagcgaaggt
     5461 tttattatgc atcagttctc tgtttatagt aaattattac tcaataatac agctaataat
     5521 gctatgatag gtcggcttaa agtgaataat cctaagaagg gtaatatcac actcttaaca
     5581 gttacggaaa aacaatttgc gagaatggtt tacctccatg gagaacgcaa cacaagtgtt
     5641 gccaactttg atagtcgctt ggttttccta ggagattctt atgatcaaga ttaattttcc
     5701 aattttagat gaaccattag tgttaagtaa tgctacgatt ttaacgatag aagatgtttc
     5761 agtttattct tcattggtga aacattttta tcaatatgac gtagatgaac atttgaaatt
     5821 atttgatgat aagcagaaaa gtctgaaggc aacagagtta atgatggtta cagatatctt
     5881 aggatacgat gtcaactcag cacctattct aaagttgata catggtgact tagaaaatca
     5941 attcaacgaa aagccagaag tgaaatcaat ggtagaaaaa ttagcagcta ctattacaga
     6001 acttatcgca tttgagtgtc tagagaatga gcttgattta gaatacgatg aaatcacgat
     6061 tttagaactc attaaggcac taggagtcaa aattgagaca cagagcgaca ctatctttga
     6121 aaaatgtttt gaaattatac aagtttacca ttatttaacg aaaaagaatc tcttggtttt
     6181 tgttaatagc ggagcttatc ttaccaaaga tgaagttata aaattatgtg aatacatcaa
     6241 tttaatgcaa aagtcagtac tctttctaga acctagaaga ctctatgatt taccgcaata
     6301 tgttattgat aaggattatt tcttgatagg cgaaaatatg gtataatatt agtaaaagca
     6361 cagtaataac aaggaatcat cgaaactgaa gtcctgctga gacgaatggc gcgattacga
     6421 aagctcaaaa gaaaattttc tacgaggttt tagagctgtg ctgtttcgaa tggttccaaa
     6481 acagcagaag aaaaagacaa ttctgtttta gagttttaga gctgtgctgt ttcgaatggt
     6541 tccaaaacct ctattctatc ttcaattgat gcacgtctgt tttagagctg tgctgtttcg
     6601 aatggttcca aaacccaagc cgttactgca aacatcatgt cgctgtttta gagctgtgct
     6661 gtttcgaatg gttccaaaac atttgaacga tttttatatt cctgatatgt gttttagagc
     6721 tgtgcggtta ttatgctagg gcaccgttgt ggtgttctag ttttttgtta tactgaaata
     6781 aattttcaga gaatgtgggg gaaggcggta attagattaa ttcaagacgt aattcagaac
     6841 ttagttggcc aagctaacga aatcacccca gtttatcagt ttgattggga aacttatata
     6901 ttggcgacta aaaaatatga acgtcattta gaggtgtgtc tattagtaga aaattcgaat
     6961 tatttttcgg attcaaagag aatgtgtcga tagaagagat acgaaagact ataattccaa
     7021 ccttatggtt aaagggctag attgttctac gctttactta attattagtt tgacagcgtt
     7081 ggttctttta gtgattgctg gtgtttcttt gtcactaata cttgcaagct tacaaggaaa
     7141 gcagtggaga acctatccat aatcaactaa cagctatgat ggatctcacc tattaatata
     7201 gttaaggagt gtccgacact ccttttttga aatacagttg gaagttacag aaaacatatt
     7261 ttcatgttat aatagtggag aaagaaatga ggtgctaata ttatggaaca aacctttttt
     7321 atgataaaac ctgatggtgt aaaacgtggt tttattggag aagttattag tcgtattgag
     7381 cgtcgaggct tctctattga tcgcttagag gttagacatg cagatgcaga tattttgaaa
     7441 agacattatg cagaactgac ggatagacca ttctttccaa cattagtaga ttatatgact
     7501 agtggtccag taatcattgg agttatttcg ggagaagagg tcatttcaac ttggcgcacc
     7561 atgatgggga gtactaaccc caaagatgcc cttccaggaa ctattcgagg agattttgca
     7621 caagctccct ctccaaacca agcgacatgt aatattgttc atgggtcaga ctcacctgaa
     7681 tctgcagctc gcgagatagc catttggttc aataactaat agacttaatg aagattgatg
     7741 ttcaagtgtt aattctcttt tacatcggtc ttctttttga tataatattc tagagtctat
     7801 aggactgttt taatatttta gaagttaaac atatcaaatt gtataggaaa tagaatgaat
     7861 attgaagatt tgaagaaacg tcaagaaaag attagaaact tttcgattat cgctcatatt
     7921 gaccatggaa aatcaacctt agcagaccgt attttagaga aaacagaaac ggtttctagt
     7981 cgtgaaatgc aggcgcaact gttggactct atggacctag aacgtgagcg tgggattact
     8041 ataaagttaa atgctattga gcttaattat actgctaaag atggtgaaac ttacattttt
     8101 cacttgattg acacgccagg gcatgttgac tttacatatg aagtatcacg ttcattggca
     8161 gcttgtgagg gtgctatact ggttgttgat gcagcccaag gaattgaagc tcaaactctg
     8221 gcaaatgttt atttggctct agacaatgat ttagagatac taccagttat taataaaatt
     8281 gatttaccag cagcagatcc agaacgtgta cgtgcagaag tagaggatgt tattggtcta
     8341 gatgcttcag aagctgtact tgcctcagct aaggcaggga ttggtattga agaaatttta
     8401 gagcagatcg ttgaaaaagt tcctgctccg actggggaag tagatgctcc tcttcaagct
     8461 ctaatttttg attctgttta cgatgcttat cgtggagtaa ttctgcaagt aaggattgta
     8521 aatggtatgg ttaagccagg agataagatt caaatgatgt ctaatggtaa aaccttcgac
     8581 gttactgagg ttggtatttt cactcccaaa gcagttggac gtgatttttt agcaactgga
     8641 gatgtagggt atattgctgc ctcaattaaa acagttgctg atacacgtgt tggtgatacc
     8701 attacccttg ctaataaccc agctatagac cccttgcatg gctataagca aatgaaccca
     8761 atggtttttg ccggtcttta tccaattgaa tcaaataagt ataacgatct gcgtgaagcc
     8821 cttgaaaaat tacaattgaa tgatgctagt ctacagtttg aaccggaaac atcacaagca
     8881 ttagggtttg gtttccgttg tggtttctta gggcttttac atatggacgt tatccaagag
     8941 cgcttggaac gagagttcaa tattgatctt attatgactg caccatcagt agtttatcat
     9001 gttaatacaa ctgatggtga aatgcttgaa gtttcgaatc catctgaatt tcctgatcct
     9061 actcgtgttg acagcattga agaaccatat gttaaggcgc aaattatggt tcctcaagaa
     9121 tttgtcggtg ctgtaatgga gttagcacaa cgtaaacgtg gtgactttgt aacaatggat
     9181 tatattgatg ataatcgtgt taatgttatt taccaaattc ctttagctga aattgtgttt
     9241 gacttctttg ataagcttaa atcatcaaca cgtggctatg ctagttttga ttatgaaatt
     9301 tccgaatatc gtcgctcaca gttagttaaa atggatattc ttttaaatgg tgataaagtg
     9361 gatgctttaa gctttattgt tcacaaagaa tttgcctatg aacgtggtaa attgatcgtt
     9421 gataagttga aaaaaatcat tcctcgtcag cagtttgaag ttcctatcca agcagctatt
     9481 ggacaaaaaa ttgtggctcg ttctgatata aaagcactac gcaaaaatgt cttagcaaaa
     9541 tgttatggtg gtgacgtttc acgtaaacgt aagttgcttg aaaaacaaaa agctggtaaa
     9601 aagcgtatga aagcaattgg ttccgtcgaa gttcctcagg aagctttctt aagtgtttta
     9661 tcaatggacg atgatgacaa aaaataattc agaaagatga tatcatttat ctattttaaa
     9721 gtaacttagc ttcgtgctta agttactttt tctttgtctg caattatcaa ataatatggc
     9781 ttt
//
LOCUS       DATRSG010000068         9715 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRSG010000068 DATRSG010000000
VERSION     DATRSG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.068"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            147..1361
                     /locus_tag="VBD89_001714"
     CDS             147..1361
                     /locus_tag="VBD89_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO7136224.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECISYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            2350..2559
                     /locus_tag="VBD89_001715"
     CDS             2350..2559
                     /locus_tag="VBD89_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HEO7136225.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2671..4284
                     /locus_tag="VBD89_001716"
     CDS             2671..4284
                     /locus_tag="VBD89_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO7136226.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGKVE"
     gene            4337..4453
                     /locus_tag="VBD89_001717"
     CDS             4337..4453
                     /locus_tag="VBD89_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136227.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            4697..5812
                     /gene="ychF"
                     /locus_tag="VBD89_001718"
     CDS             4697..5812
                     /gene="ychF"
                     /locus_tag="VBD89_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO7136228.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            5896..6471
                     /locus_tag="VBD89_001719"
     CDS             5896..6471
                     /locus_tag="VBD89_001719"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO7136229.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKKLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     SNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            6468..>9715
                     /gene="mfd"
                     /locus_tag="VBD89_001720"
     CDS             6468..>9715
                     /gene="mfd"
                     /locus_tag="VBD89_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO7136230.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEEQRAYLSEVLSTTENHFKHSNIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNVELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFT"
BASE COUNT         3396 a         1429 c         2024 g         2866 t
ORIGIN      
        1 gatacctatt caagattaga agcaatttta tatccataca cacaaagcag ttagtcttag
       61 gattgactgc tttttctata cagattttta atgacaggca attatcaatt atggtagttg
      121 ccttttttga ttggaggaat tgaagaatga atgataaaaa ctttattgaa gaactaagac
      181 aaaagcgtga ggaatacgga gtaacacaaa caaggcttgc tgttgcctgt ggtatcagcc
      241 gtgaatatta caaccgcatt gaaaaaggaa aacagccatt gaatgacgaa ttaagagaaa
      301 tcatagaaaa acagattgag cgtttcaatc cgcaagaacc actattctta ttgattgatt
      361 actttcgtgt ccgctttcct actacggacg cattggcgat tatccgtgat gtattacaac
      421 tgaaatctaa ttacatgctt tatgaagatt atggaaaata cggatatgaa agcaaatatg
      481 tacttggcga catcaatatc atgtgttcca tgcaggagca tttaggtgtt ttattggaac
      541 tgaaaggcaa aggctgtcgg caaatggaat gttatctact ggcacaggaa cgctcatggt
      601 atgacttcat gctggattgt atgacggctg gtggtgtgat gaaacggctt gatttggcta
      661 tcaatgataa agcaggaata ttggatattc caaagttaaa ggaaaaatac aaggctggcg
      721 aatgtatctc ctacttccgt atgcagaaag attacagcgg tacagaaaaa tgcggtagcg
      781 atttaccaaa gaatacagga gaaaccttat atctcggttc aacaagtagc gaattatata
      841 tgtgtgctta tcagaaaaat tatgagcagt atgtcaagaa tggcacagaa attgaagata
      901 cggagattaa gaaccgtttt gaaatacgca tgaagaacga acgagcctat tatgcggttg
      961 tagatttact gacctatcgg gacgctgaac gcaccgcttt ttctatcatc aatcattatg
     1021 tccgctttgt tgacagagag gacgacaagc caaaaagtca atggataaca aatgatgatt
     1081 gggcatggtt tgtaggggaa aacagagagc cgatacgctt aaccacaaag ccagagcctt
     1141 acactttaca aaaagcgttg cattggctac aaagacaagt cgcaccaacc ataaaaatgg
     1201 tacaggcatt agacagagaa aatcgtacaa cgatactgaa agatatgatt gagcaggcag
     1261 aactaaaaga caagcataaa catttattac aattagagaa atcaaccata gaagaacgta
     1321 tagataccgc tgttccacaa gagaatgacg gtatttttta atgtaattga aaaatttcag
     1381 atttagtcag caaagagctg ttttcattcc ctatgtgaag tgaaagaaga aaaataaatt
     1441 ttttattaaa tctgcaacaa aatggctact tgcgtacaga tatggtgcga gaaaaggaaa
     1501 tctaaacttt ttttgaaaat aggtcattaa atcggggctt gctgtcccta tatgaagtga
     1561 aagaagaaaa aagttttgaa aaaatgacgg aaattcgtga ttagtggaca gtcttctgtg
     1621 aaaagaggaa aaacatcatc attttgtatt caaacaggca ttagcagttg agtgttgatg
     1681 tgaaaagatg aaaatacttt gttctcaact taacaattta aggtttccgt tccctatatg
     1741 gtgcaaagaa aatatttcat tctattgttg gcagtaacgg tatggtttgg gtagttaggg
     1801 tgtgaaaagg aaaatattct tttttcatca tcaacttagc aaaatgacat tttctatccc
     1861 tatatggtgc atgaaaagaa gttaaaattt ttccatttaa acttagcaaa tggacgtttg
     1921 ctgtacagat agtagtgaaa aaggagaaaa tcaaccgttg attgccaaaa tgaaccgttc
     1981 catttgtact aataatggaa aaagttttgg attttggtta cgtttctcct cttttccaac
     2041 gcgatatata ggaaagacga tttttttctt tcaatcgttc tttgacaact gaataaagta
     2101 attcaatacg tttgacagac agctagccgt tgaaacaaat acgccacgac ctttcccctg
     2161 caagagcgag cgaaaaccta tttgtgttct tgtaaaagat ttgtcctctt ttatcgccat
     2221 gacctgtact gtcagataat gatactcccg tacagccaca gcttgagcat taagagcgtc
     2281 gcacgcaatg ggtacggcta cataagaact atgcagaggt ggaactcctg tgggctatga
     2341 caaaagccgt tgaattgctt agaaagattt tacagaaagg gggtatgtgt tattgataat
     2401 gctgtaaaaa caattcaaaa gaaaaatggc aagtgtggca ttggcaacag aggaaagaca
     2461 aagattgtag taaaagagca tttttccgaa aagggcaaaa caatggaaga acttctaact
     2521 gatgtcatgc tggaaaaagc aaagcagaca atcgcataaa atcggtgcag ttgtaagaaa
     2581 tagattgaat gacaaaaagt acccgtgtta tactttactt gcaagcaggt attgtaaaca
     2641 cgggttagtt ataaaggagg ataactgaca atgaaacaac agatttacaa tactgcactt
     2701 tatcttaggt taagccgaga tgatgaatta cagggcgaaa gttccagcat taccacacag
     2761 agaagtatgt tgcgtttata tgcaaaagaa catcatttga atgtcattga tgaatatatt
     2821 gatgacggct ggtcgggaac aaattttgac agaccgagtt ttcaaagaat gattgaggat
     2881 atagaggcag gaaaaatcaa ctgtgttgta acgaaagacc tttcccgtct tggcagaaat
     2941 tatattatga caggacaata tacagaattg tatttcccta gtcataatgt ccgctacata
     3001 gcgattgatg acggtgtaga cagcgaaaaa ggcgaaagtg agattgcacc atttaagaac
     3061 atcatcaatg aatgggtggc aagagataca agccgtaaag tcaaatcagc atttaagaca
     3121 aagtttgcgg agggggctca ttacggtgct tatgctccgt taggatataa gaaacaccct
     3181 gacatcaaag gaaaactgtt ggttgatgat gaaacaaaat ggattattga aaaaatcttt
     3241 tccctagcct atcaaggtta cggaagtgca aaaatcacaa agcagttaag agcagaaaaa
     3301 gttccgacag cgtcatggct aaattttaca agatacggta cttttgcaca tatctttgag
     3361 ggaaaacccg aaagtaagcg ttatgagtgg acgattgctc atgtcaaggc gatattgaaa
     3421 agtgaggttt atatcggaaa cagtgttcac aatatgcagt ctacggtatc gttcaagagc
     3481 aaaaagaaag tgcgtaagcc cgaaagtgaa tggtttcgag tagaaaacac tcacgagccg
     3541 attattgaca aggaagtgtt ctatcgtgtg caggagcaga taaaatcaag acgcagacag
     3601 acaaaggaaa aggcaacgcc gatttttgca gggcttgtca agtgtgcgga ttgtggttgg
     3661 tctatgagat ttggaacgaa taagacaaat aaaacgcctt acagttatta tgcttgcagt
     3721 tactacgggc agtttggcaa aggtaattgt tctatgcact acattcgtta tgatgtgctg
     3781 tatcaagccg tattggaacg cttgcagtat tgggctaagg cagtacagca ggacgaagaa
     3841 aaggtattga acaagataca gaaagctggc aatgcagaga gaatacggga aaagaagaaa
     3901 aaggcaagta cattgaagaa agccgagaac agacaaaatg agattgaccg tttatttgcg
     3961 aaaatgtatg aagatagagc ctgtgagaag ataacagagc gaaattttgt gatgttgtcc
     4021 agcaagtacc aaaaagaaca gatagaactg gaacagcaga taacaagcct aagagaagaa
     4081 ctaagtaaaa tggaacagga tatgataggt gctgaaaagt ggattgagtt aatcaaggaa
     4141 tattccgtac caaaggaact gacagcacca ttattaaatg ccatgataga aaaaatcctc
     4201 atacatgaag caacaacgaa tgaggataac gaaagaatac aggaaattga gatatattac
     4261 cgatttattg gaaaagttga gtaatcaaca agagtaatat ttttaactaa gggaaaccgg
     4321 ggataccaca ccagatttgg ataatttaat caggttagct gagatttttg acgtgagctt
     4381 agatgaacat gttttagcta aaacaaatga agttaaagtt gaacgtattt atgaaaaaaa
     4441 tcttgatctg taaaaatata ataaaattta ttggtttatt tttcgaaata ttattcgatc
     4501 tctactaatt atcttagcta tattaacttt cttataagct ctaggtcttc cctttgtttc
     4561 ggagtggata atttaaatga aggtgaaaga gtggcataat gataagcaaa tctgaaattt
     4621 tcttcttatc ataccgcttt ttcttctatt ttttgatata atggtcttga ttgaaatttg
     4681 aatggagact cactaaatgg ctttaacagc aggtattgta ggcttaccaa atgttggtaa
     4741 atcaacttta tttaacgcaa ttacaaaagc aggagcagaa gctgctaact atccttttgc
     4801 gactattgat cccaatgttg gtatggtaga ggtaccagat gagcgtctgc aaaaattgac
     4861 agaattgatt acgcctaaaa aaacagttcc gacaaccttt gaatttactg atattgcagg
     4921 tattgttaaa ggtgcttcga agggggaagg tctaggaaat aaatttttag ctaatattcg
     4981 tgaagtggat gctattgttc atgtggtacg tgcttttgat gatgaaaatg ttatgcgtga
     5041 acaaggtcgt gaggatgcct tcgttgatcc tatagcagat attgatacta ttaatcttga
     5101 attaatctta gctgatttag agtcaatcaa taaacgttat gcgcgtgttg aaaaaatggc
     5161 acgaactcaa aaagataaag aatcagtagc agagttcaat gttttacaaa agattaaacc
     5221 tgttttggaa gatgggaaat cagctcgtac cattgagttt acagaagagg aagcaaaagt
     5281 tgttaaaggt ctctttttat taacaactaa acctgtttta tatgttgcta atgttgatga
     5341 ggacaaagta gctgatcctg atgatatcga ctatgttaat caaattcgtg catttgcaga
     5401 aacagagagt gcagaagtag ttgtgatttc tgctcgagct gaagaagaaa tatctgaact
     5461 tgatgatgaa gataagttag aatttttaga agctattgga ttaacagaat ctggtgttga
     5521 taaattaaca cgagctgctt atcatctttt aggccttgga acttacttta ctgctggtga
     5581 aaaagaggtt cgcgcttgga catttaagcg tggtataaaa gctccacaag ctgcgggtat
     5641 tatccattca gattttgaaa gaggttttat tcgtgcagta acgatgtcat atgatgacct
     5701 tattcaatat ggatctgaaa aagcagtaaa agaagctggc cggttacgtg aagaaggaaa
     5761 agagtatatt gttcaagatg gcgatatcat ggaattccgt ttcaatgtct agtaataaat
     5821 aatcaatttg ttaactttta ggttaaggat attgttatta tccttaacca tttttgcgtt
     5881 ttaatggaga agataatggt aaaaatgatt gttggattag gaaatccagg aagtaaatat
     5941 aatgatacaa aacataatat tggatttatg gctgttgaca gaattgtaaa aaaacttgac
     6001 gtcaacttta cagaagataa aaattttaaa gcagagatag gtagtgattt tatcaacggt
     6061 gaaaaaattt attttatcaa accaactact tttatgaata acagtggtat tgcggtaaaa
     6121 gccctgctta cttactacaa catttctatt aaggatatga ttatcatata tgatgattta
     6181 gacatggaag tcggtaaaat acgttttcgt caaaaaggat ctgctggggg acataatggt
     6241 atcaagtcta ttattgctca tttaggtact caagaatttg atcgtattaa agtaggtata
     6301 ggacgtccga atggacgtat gacagttatt aatcatgttc tagggaagtt tgataaaaat
     6361 gatgaaatta tgatatcaaa tactcttgat aaggttgaca atgctgtcaa ttattattta
     6421 cagacgaatg attttcaaaa aacaatgcag aaatacaacg gtttaaaatg aatattatag
     6481 aattatttag ccaaaataaa gtggtgcgga catggcactc tggattagta actaatagtc
     6541 gccaattagt tatggggttt tcaggagcaa gtaaagctat tgctatagca tcagcctacg
     6601 agaagttatc taaaaaaatt atggtagtca ctgccactca aaacgattct gataaattat
     6661 caagtgatat atcaagttta attggagaag ataacgttta tcaatttttt gctgatgacg
     6721 ttcctgcagc agagtttatt ttctcttcat tagacaaatc aatttcacga ctcagtgcct
     6781 tgaggtttct aaaagatcca gaaaaaaacg gtgttttgat tactagtatc agtggtttaa
     6841 ggttactatt accaaatccg gaagttttct cgaaaagtca atataagttt gaaatcggtc
     6901 aagaatgtta tttagataaa ttgagtaaga acttagtcaa tttaggttat cagaaagttt
     6961 ctcaagtatt tagtcctggt gaatttagcc aaagaggaga tattttagat atttttgaga
     7021 tgacacaaga atatccttat cgcttggaat tcttcggaga tgaaatagat ggcatcaggc
     7081 agtttgatat tgacactcaa aagtctctta aacaattaga gtcagtacag atttcgccag
     7141 cagatgatat tattttacaa gatgcagatt tcgagcgtgc aaagaaaaaa cttgagggct
     7201 atctagtaac agctagtgag gaacaacgcg cctatttgag cgaggtttta tctacaaccg
     7261 agaatcattt taagcactca aatattcgtc gttttctaag tatcttttat gaaaaagaat
     7321 ggggaatatt agactatatt cctgagggaa ctccactttt tgttgatgat tttcaaaaaa
     7381 ttgtagatcg aaatgctaag ttagatttag aaatagctag ccttctgacg gaagatttac
     7441 aacaaggcaa gtctcattct agtttgaact atttttcaga tccttataag caattaagac
     7501 aataccaacc agcaaccttc ttttcaaatt tccacaaagg attaggaaat ctgaaatttg
     7561 ataaacttca tcattttact caatatggaa tgcaagaatt ttttaatcaa tttccattgt
     7621 tggtcgatga aatcaatcgt tataagaaat ctggagcgac agtactgttg caagtagatt
     7681 ctcaaaaagg tttaaatctc cttcaagaaa acttaaaaga atatgggtta gatttaatca
     7741 tttcagataa aaacgatatc gttccaaagg agtcacaact tattgttggg catctttcaa
     7801 atggttttta ttttgcagat gagaaaattg ttctgattac tgaacgtgag atttatcata
     7861 ggcgtgttaa aaggaaaatt agacgcagta atattagtaa tgcagaacgt ctaaaagatt
     7921 ataatgaact ttcagttggc gattatgttg ttcataatgt tcatggcgta ggaaaatttt
     7981 taggtattga aacaattgag atacaaggta ttcatcgtga ttatctgact attcaatatc
     8041 aaaatgctga tcggatctct atccccgtag aacaaattga acttctaaca aaatatgtct
     8101 ctgcagatgg caaagagcct aaaattaata cgttaaatga tggtcgcttc aaaaaagcaa
     8161 aacagagagt tgcaaagcaa gtagaagata ttgctgatga tttgttgaaa ctttatgcag
     8221 agcgtagtca attacaagga tttgcatttt ctcctgacga taatatgcaa aatgactttg
     8281 ataatgattt tgcatatgta gagacagagg atcaattgcg gtcgataaaa gaaattaaac
     8341 aggatatgga aggtaatcgt ccgatggatc gattattggt tggagacgtt ggttttggta
     8401 agacagaggt tgctatgcgt gcggctttca aagctgtcaa tgaccataaa caagttgctg
     8461 ttttagtccc aacaacagtt ctagctcagc agcattttga aaattttaaa gaacgtttta
     8521 gtaattatcc tgttacagtt gatgtactta gtcgttttcg tagcaaaaaa gaacaaacag
     8581 atacactaaa aagattatct aagggccaag ttgatattat tattggaaca catcggcttc
     8641 tctctcaaga tgttgtattt tcggatttag ggttaatagt tattgatgag gaacagcgat
     8701 ttggtgtaaa gcataaagaa aagttaaaag aattaaaaac aaaagttgat gtcttaactt
     8761 tgacagcgac cccaatacct agaacattac atatgtcaat gcttggcatt agagacttat
     8821 cagtcattga aacacctcca actaatcgtt atcctgtgca gacttacgtt ttggagacaa
     8881 atcctggttt agttagagaa gctattattc gtgagataga cagaggagga caggtttttt
     8941 atgtttacaa taaagttgac actattgaac agaaagtttc agaacttcag gagttagtac
     9001 cagaagctag tattggtttt gttcatggtc aaatgtctga aattcaatta gaaaatacat
     9061 taatagactt tatcaatgga gattacgatg ttttagttgc aacgacaata attgagacag
     9121 gagtcgatat ttcaaatgtt aataccctct ttgttgaaaa tgcagatcat atgggcttgt
     9181 caacccttta tcaattaaga ggccgtgtgg gtagaagtaa tcgcattgct tatgcttacc
     9241 ttatgtacag acctgataaa gttttaacag aaatttctga gaaaagacta gatgcaatca
     9301 aagggtttac agaattggga tctggtttta aaatagcgat gcgtgattta tctatacgtg
     9361 gtgcaggaaa tattttagga gcttcacaaa gtggttttat tgattcggtt ggattcgaga
     9421 tgtattcaca gcttctagag caagcgattg ccaccaaaca aggaaaatca ttaattcgtc
     9481 aaaaaggtaa tgttgaatta gccttacaaa ttgacgccta tttaccagca gaatatatat
     9541 cagatgagcg ccaaaaaata gaaatatata agcgtattcg tgaattagaa actagggctg
     9601 attatgaagc cttacaagat gagttaatag atcgttttgg agaatatcct gatcaagttg
     9661 catacctttt agaaatagga cttttaaaag cttatctgga tcttgccttt actga
//
LOCUS       DATRSG010000069         9415 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRSG010000069 DATRSG010000000
VERSION     DATRSG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.069"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(27..1499)
                     /locus_tag="VBD89_001721"
     CDS             complement(27..1499)
                     /locus_tag="VBD89_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7136231.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     IPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(1730..2566)
                     /locus_tag="VBD89_001722"
     CDS             complement(1730..2566)
                     /locus_tag="VBD89_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7136232.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(2566..3936)
                     /locus_tag="VBD89_001723"
     CDS             complement(2566..3936)
                     /locus_tag="VBD89_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7136233.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(4034..5281)
                     /locus_tag="VBD89_001724"
     CDS             complement(4034..5281)
                     /locus_tag="VBD89_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7136234.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            5537..6565
                     /locus_tag="VBD89_001725"
     CDS             5537..6565
                     /locus_tag="VBD89_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7136235.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            6686..8182
                     /gene="malQ"
                     /locus_tag="VBD89_001726"
     CDS             6686..8182
                     /gene="malQ"
                     /locus_tag="VBD89_001726"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO7136236.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            8194..>9415
                     /locus_tag="VBD89_001727"
     CDS             8194..>9415
                     /locus_tag="VBD89_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEO7136237.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNK"
BASE COUNT         3200 a         1527 c         1774 g         2914 t
ORIGIN      
        1 tgtaacctag ttctttttag atgtctttat ttaactccag acattagttt ttcaatgcgt
       61 ggaacataaa ataagaggat aaatccaaat acaactgtta taccaccaac aacaccataa
      121 taaagaattt gagtatcagg tgtgtacaat ttaacgattt gagcgttaat agcttgtgct
      181 gcagcattac ttaagaacca gatgctcatc atttgagctt ggaatgcttt aggtgcgagc
      241 ttactagttg ctgataatcc aactggtgag attagcattt ccccaacgat gacaatagcc
      301 caactcattg ttaaccaaag agggcttact ttagcgttaa ctccaaagag taaacctggt
      361 agcatcatcc ataagaatga agctccagca aagaaaagtc catacgcaaa ttttttaggt
      421 gatgaaggtt gcttacttcc ccatttagcc cataaccaag caaagaatgg tacataaagc
      481 ataatgaaga gagggttcat actttggaaa taacttgatg ggaaattaat atgatgccca
      541 aagaagttaa ggtaaagttt tgtttgttca tctgcaaata aggctaagac aactgaacct
      601 tgttcttcaa ttgaccaaaa tagaattgag gcgataaaga gagggatata tgcccacact
      661 cgagaacgct caacagaaga aatcttttta ctacttaaaa tcttgataaa ataaataatt
      721 gggatggcga ttgcaataat actaaaaata tcgataatta cttcaattgt tagcatatgc
      781 atcaagtgaa gtagaataag tacaataaca ataccaataa ggataagccc aactttttta
      841 tatagagagg acttttcttc aggacttaaa ggatcgtttg gtctgagact ctcttcagtc
      901 aaatattttt ttccatctaa ggtatattgg aggagaccaa agaacatacc aatagcagca
      961 agtgagaaac caagatggaa atttacttct tgtccgaggt accccactac aatgggtgaa
     1021 ataaaagcac caaggttaat accaaaaaca aagatactaa aaccagcatc acgacgagaa
     1081 tcattttctc catataaatt accaaccatc tctgatacat ttggttttaa tagtccagta
     1141 ccaaggataa ttagagcaat cgaaatgaat agagctgttt gaccaaatgg tatagctaga
     1201 gcaatatgac ctagcatgat tagaatacca ccatacagaa cagttttacg actacctaga
     1261 atacggtcgc tgacaaaacc accaattact gatgagagat aaaccagtga accatagaca
     1321 gccatgattg atgcagcgac agtcttgtcc ataccaaggc caccttgaga aacactatag
     1381 tacatatagt acagcaaaat agcacgcata ccatagtatg agaatctttc ccacatttca
     1441 gtaaagaaaa gagtggacaa accacgagga tgtccaaaga atgattttgt tttttccatg
     1501 aaaaacgctc cttttgtaga taacactatt aataatatag taaatagaag caactgtcaa
     1561 attacttagc actatcaagt tatttttttg tagaggttaa cacccttggt aaaccaagtt
     1621 ttttaactat ctagcttaga aaaaggcttg aattcaatag aacccaagcc ttttttagaa
     1681 aagaaaccac agtgaaataa aaatgtcgtg tctttaaatt atctgaagat tattttacag
     1741 atccacctgt aataccttca acatagtatt tttgcatgaa catgaaaagt attgtgattg
     1801 gaattgcaat aataacagat cctgctgtaa atgacatgaa ccactggttg atggtatctt
     1861 gttgcaacat tgaaaagaga ccaatagcga ctgtgtactt gctcgtagca tcacctaaaa
     1921 taacttttgc aaagataaag tccatccaag ggcccataaa agcgattaat gcagtataaa
     1981 cgataatggg ttttgacaat ggtagggtga ttttaaggaa aatatccaaa cgggtcgccc
     2041 catcaatcat agcagactca tccaaagagt atggaatagt atcaaagaaa ccttttgcga
     2101 tgtagaaggt taatgctgct cctgcagaat agacaaatat taaagcggtt aatgtttggt
     2161 ctagatttag agcttttagg atgtagtaaa ctgcaatcat tgacatgaac ccaggaaaca
     2221 tattcaagac taaagctagc tttaggaaac cattacggtg tttgaatttg attctgctga
     2281 gagaataagc cattgctaca gtgattaaag ttgatagtat acaagtacat gtagcgacaa
     2341 ataatgtatt taagaaccat tgaccaaatg ggaatgtgtt ttgggtgaag agtttagcgt
     2401 agttgtctaa tgtccaagtt ttaggaataa agtagttgac aaaggcactt ccttcaccac
     2461 ggaagcttgt gagaacgacc caaacaatgg gaaagagcca cataattgat agtactatca
     2521 ataaaatata aacaaaagta aggttaagac gttttttctt attcattatt tagctgcccc
     2581 ttccttgtaa gaatttgttc ttgtgtaagc tagtaaacta aatactgctg aaagaataaa
     2641 gattaagata ccaacaacag atgctaagtt ataatctgca gcagttactg tcaaattgta
     2701 tagccaagtg acaagtaaat cggtgctacc tgcttggtag tatgttgagt ttgttggtcc
     2761 accttgagtg agtaggtaga taacgttgaa gttgttaata tttccgataa attgttgaat
     2821 taaagtagga gtcataatca acaaaatttg tgggaaagta attgattgaa agacttggaa
     2881 cttattcgcc ccatcaattt cagcagcttc aatttgttct gcaggtaaat tcatgatgat
     2941 accagtagca acaagcattg ttacaggaat accaacccac atattaacaa agatgattga
     3001 gaatttcgcc cagaccggat cagaaaggaa tggaagggga tgtgctgaag aaataagtcc
     3061 ccattttatt aacaaggcgt taaccggtcc agcgtcactt aagaggtttc tcatgattaa
     3121 gagagagata aattgaggga cagccatggt aataacaaaa atagtacgcc acatcttttt
     3181 gaatttcaaa ccttttgtat taattagtaa ggcaagaata ataccaaaga agaaattcgt
     3241 tacagtagcg aaaacagccc aaattaatgt ccaagataga acagggaaga aagtactggc
     3301 catacgcccg ctaaagatat taccgaagtt agcaaaacca acccagtcaa ataaactttt
     3361 tggcggtaag tggttatgat catagttagt aaatgccaaa caaatcatat aaactaacgg
     3421 aaggattgta aatagaagca cccctattaa aggaatagac attaaagtca tatggaagcg
     3481 tccatctgtc aagcttttaa tatctttttt gaaggatgga atttctcttc cttcttcttt
     3541 caaggtcaat aaatgtgctg cgcttttaat gtttgaccag taaatatagg caaatacgca
     3601 acagaagata agcgaagcta atccaaaaat tagcattaac atagagttat caccttgtgt
     3661 cgcaacttgg attttaatac catccacagt tttagttgtc ataccttgtt cttgggtccc
     3721 taaggaaata agacctttta cagctgggat aacttggtaa acaaaggtaa ttaaaaacag
     3781 aatttcactg attaaaaata aaagtccctt gacaatttgt ttatttttga ggttagcaag
     3841 tcccataatt aagaatgata gtttagttgc taaatcacct ttttgaaaga cctcttttag
     3901 ggacatacca tcaagtgaga tcagtttttt attcataatg gtctccttta attgttattt
     3961 tacaaaataa gagatgggaa gcactctcaa ttgaaaggaa gagtactcac ctcatctcat
     4021 tactctagac gtactatttt gttttagcga tgtctttatc gaattgtttt agacgtttaa
     4081 ggtaatcgct agatttgatt ttaccactgt aagtatcgct aagaatagca gcactttctg
     4141 tccagaatgt tgacatttga ctcaacttag gcataaccgt tgtatattta tctgagctac
     4201 ccatttcgat aactgctttt gcaagttcat ctttttggac gctatcagaa gattgaattg
     4261 atgagttagc aggaacgata tgacgttttt cgaattgaat tttttgactt tcagcattag
     4321 ttagatatgc agcgagtttg tagctagctg agattcgttt agtgtttgaa ccagcaggtg
     4381 cttggttaac ggcataaagt ttaacgccca agaatgcttt ttgttgaact tctttgtcac
     4441 cgattttcat tgttgggtaa acagcaacac cgattttatc ttcaccgaca gcttttttag
     4501 cagcgtcgta atcccatggt ccactttcaa aagcatgaac agaaccatcg ccaaatttag
     4561 acattgtatt ttcagctgtc aagttgacaa aaccatcatt tttcttttga tctgcaatcc
     4621 atttaaggac agaaacacct gcttcattac cccagttagt gcctttagca tcttcaccag
     4681 atttaccaaa taaagtgtcg cctacagaaa ggaaaagagg acctgttaca tatgagttag
     4741 ctgctttaag ctgttgaccg aatttccctt tgcttgtaat tgtttcgtat gatttaacgt
     4801 cgtcagcagt taactttgtt ttattataat aaagaacttg agattcaata ccaaatggga
     4861 atgcataagt tttcccttta tattgtgcac cagtaagtga ttgtttagtg tcgtttttag
     4921 caatttcttt tgagtattgc tctggaattt cttggataac accagattct actaattgac
     4981 caagttggtc atgtggaagt gagaatacat cggctgcctt gcttgggtct ttttttacgt
     5041 tttcttgagc tttggagtca ttagactcaa tcatttttac agtaacgcct ttgttttcct
     5101 tctcgaattt tttaacaatt gctttataag acgcttttga atctgttggg acccaaagtt
     5161 tgatagttgt ttttgaagca gcatcaactg agttagagtg agtagcagca attgctcctc
     5221 ctgcaactac tgaaagagca gcagtactaa caagtaattt tttccaagta tttttttcca
     5281 ttttggttct cctcctagag aatattttaa ttacacctat attatgcaac cgtttgcaaa
     5341 aaaagtcaag tcttttttta aaaaaaataa gaaaaataaa aaaataatat caattaatac
     5401 taaatgagaa gattatttta gtgagagtat tacatttgca gagaaggcaa acgtttgttt
     5461 ttttcttaga ttagagtata atagacaaag gactatacca aaaggtataa aatgattaga
     5521 atgaagaggt cgtaggatgg taactataaa agatgttgct gccaaagctg gtgttaatcc
     5581 atcgacagta agtcgtgtac taaaagataa tgaatctatt tcaagtaaga cgaaagagcg
     5641 cgtgaaaaaa gctatggaag agctgggcta tgttcctaat gttgcagcac aaatgttggc
     5701 tagtggttta acgcaaaata ttggagttat ttttcctcca ctggttttac cagaccgctt
     5761 aagtgaaccc tttttcatgg agattctttc tacattaact aatgaagcaa aagagcatgg
     5821 gtttacagtt tctattgcaa caagtatgtc ttctgaggag ttgttagaac aagtaaaatt
     5881 aatgcatcta caaaaacgag tggatggttt tattgttctc tattcggatc aaaaagatcc
     5941 tatcagagag tatttattaa aatataaact gccctttgtt gttgttggtg ctgctaatgg
     6001 ctatgaaaat gagattactt atattgataa tgataatcag ctgatgggga aatctgccat
     6061 tgattatctc tataaaaaag gacataaaag catccagttt gttacagatg atttaaattc
     6121 agaagtatca gaagagcgct atttagggta ttttaaaggt gctagaaagt taggtttaaa
     6181 ccaaaagcca gctcttttat ttgacagagg caatccgcaa gttttagagg aatttataaa
     6241 tagagtcaaa gaagaagaaa caactgcttt aattgttatt ggtgatactg tctctgttag
     6301 agtaatgcaa tttctttctt tctataaatt aaaagttcca gatgacattt ctatcatgac
     6361 ctttaataat tctctcttct cacatctgat tcatccttac ctaagcacat ttgatattaa
     6421 tgttaataat ctaggaagga caagcgttcg tcgtctgatt gacatcatta aatctccaga
     6481 caaagtcttt agtgagacta ttattgtgcc ttttacttta gaagagcgtg aaagtgtccg
     6541 agatatttct aaaaataata attaagctat gatataaaga cttctatcaa ttttcgatag
     6601 aagttttttt aaattatgct tgacttatgc aatcgcttgc gttaagatgg aggagtaaag
     6661 taagtaatcg aaaaggagaa tcaatatgaa aaaacgtgca agtggtgtct taatgcacat
     6721 cacttctcta ccaggtgatt taggtattgg aacatttgga cgagaagcct acgcttttgt
     6781 tgattttttg gtcgaaactg atcaaaaatt ttggcaaatt cttcccttaa caacaacaag
     6841 cttcggggac tctccttacc agtctttttc tgcagtagct ggtaatacac atttaattga
     6901 ttttgatttg ctaacgcttg aaggtttcat ttcgaaagat gactaccaaa atattagttt
     6961 tggacaggat ccagaagttg ttgattatgc tggtctgttt gaaaaacgcc gtccagtttt
     7021 agaaaaagca gttaaaaatt tcttgcaaga agagagagct acgagaatgc tatctgattt
     7081 cttgcaagaa gaaaaatggg taactgattt tgctgaattt atggcgatca aagaacattt
     7141 tggtaataag gcgcttcaag aatgggatga caaggctatt atacgccgcg aagaagaagc
     7201 cttagcagga tatcgtcaaa agcttagtga agtgataaaa tatcatgaag taacgcaata
     7261 tttcttttac aaacaatggt ttgagttaaa agaatatgct aatgataaag ggattcaaat
     7321 tatcggtgat atgccaatct acgtttctgc cgatagtgta gaagtttgga caatgcctga
     7381 actgtttaaa ttagattgcg acaaacaacc acttgctatt gctggtgtac cagcggatga
     7441 tttttctgac gatggtcagc tttggggcaa tcctatttac aattgggatt accacaaaga
     7501 gtcagatttt gactggtgga tttatcgtat tcaatctggt gttaagatgt atgactattt
     7561 gcgtattgat cattttaaag gtttttcaga ttattgggaa attcgaggag attatcaaac
     7621 agctaatgat ggctcatggc aaccagctcc cggtccagaa ttatttgcaa ctatcaaaga
     7681 aaaattaggt gatttaccta ttattgctga gaatttaggc tatattgatg agagagcaga
     7741 gagattactg gctggaacag ggttcccagg tatgaaaatc atggaattcg gtttttatga
     7801 tacaacagga aatagtattg atattcctca taactacaca gaaaatacaa ttgcatatgc
     7861 tggaactcat gataacgaag ttattaatgg ctggtttgaa aatcttacag tcgaacagaa
     7921 agcttatgca gaaaattaca tgcgtcgttt gccaaatgag cccattactg agactgtact
     7981 gcgtaccttg tatgcaaccg ttagtcaaac aacgattaca tgtatgcaag atctactaga
     8041 taaacctgca gatagccgta tgaacatgcc aaataccgtt ggaggtaact ggcaatggcg
     8101 tatgagaaaa gaagacttaa cagaaaatcg caaagcattc ttaaaagaga ttacaactat
     8161 ttataataga gggaataaat aaggaatatt atcatgacaa gaaattttac aacttacgtt
     8221 ggacaacaag gaaaagtatt atcagaatta acaaatgaag agatttacgt tgaattgtta
     8281 aattttgtaa aagaagaagc tgccgctaaa tcaaaaaata gctctcaacg taaggtatac
     8341 tatatttcag ctgagtttct aattggtaaa ttactttcaa ataatttgat taacttaggg
     8401 atttataaag atgtcaaaaa agagttagaa cttgtaggta aatcaattgc tgaaatagaa
     8461 gatgtagagc cagaaccatc acttggtaat ggtggtttag ggcgtttagc atcatgtttt
     8521 attgactcga tttcttcttt aggtattaat ggtgaaggtg ttggtttaaa ctatcactgt
     8581 gggcttttca aacaagtatt tcgcaacaat caacaagaag cagaagctaa ctactggatt
     8641 gagaataatt cttggttagt accaacagat atttcttatg atgttccttt ccgtgacttt
     8701 actttaaaat cacgcctaga tagaattgat gtattaggtt ataagaaaga tactaaaaat
     8761 tatttgaacc tttttgatat tgatggactt gattataacc ttattgaaaa aggtatcact
     8821 tttgataaga cagaaatcaa gaagaattta accttgttct tgtatccaga tgattcagat
     8881 aaaaatggag aattattacg tatctatcag caatatttca tggtctcaaa tgctgctcaa
     8941 ttattaattg atgaagcaat cgagcgtggt tcaaaccttc atgatttagc tgaatatgct
     9001 tatgttcaaa ttaatgacac tcacccatct atggtcatcc ctgagttgat tcgtttattg
     9061 acagaaaaac atggttttga atttgacgaa gctgtgtcag ttgttcgcaa tatggttggc
     9121 tatacaaacc ataccatcct tgcagaagct cttgaaaaat ggcctttaga gtatcttaat
     9181 gaggtagtac cccacctagt aacaatcatt aaaaaattag atcaaatgat tcgtgaagag
     9241 caaacaaatc cagaggttca aattattgat gaagctggac gtgttcacat ggcgcatatg
     9301 gatatccact tctcaacaag cgttaatggt gtagcagcat tacatacaga aatccttaaa
     9361 aatagtgaat taaaggtttt ctatgatatt tacccagata agtttaacaa taaga
//
LOCUS       DATRSG010000070         9316 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRSG010000070 DATRSG010000000
VERSION     DATRSG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.070"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(46..1365)
                     /locus_tag="VBD89_001728"
     CDS             complement(46..1365)
                     /locus_tag="VBD89_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO7136238.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
     gene            complement(1519..2016)
                     /locus_tag="VBD89_001729"
     CDS             complement(1519..2016)
                     /locus_tag="VBD89_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO7136239.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTADVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            complement(2152..3516)
                     /gene="radA"
                     /locus_tag="VBD89_001730"
     CDS             complement(2152..3516)
                     /gene="radA"
                     /locus_tag="VBD89_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO7136240.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            complement(3678..4124)
                     /locus_tag="VBD89_001731"
     CDS             complement(3678..4124)
                     /locus_tag="VBD89_001731"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO7136241.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            complement(4187..4272)
                     /locus_tag="VBD89_001732"
     tRNA            complement(4187..4272)
                     /locus_tag="VBD89_001732"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4236..4238),aa:Leu,seq:aag)
     gene            complement(4319..5245)
                     /locus_tag="VBD89_001733"
     CDS             complement(4319..5245)
                     /locus_tag="VBD89_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7136242.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTVVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            complement(5354..6958)
                     /locus_tag="VBD89_001734"
     CDS             complement(5354..6958)
                     /locus_tag="VBD89_001734"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO7136243.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            complement(7231..7806)
                     /locus_tag="VBD89_001735"
     CDS             complement(7231..7806)
                     /locus_tag="VBD89_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO7136244.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEEEEEEEEEEEV"
     gene            complement(7995..9278)
                     /locus_tag="VBD89_001736"
     CDS             complement(7995..9278)
                     /locus_tag="VBD89_001736"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO7136245.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGVAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
BASE COUNT         2844 a         1978 c         1429 g         3065 t
ORIGIN      
        1 gataaaaaaa gtaaagcaaa attgccttac tttttaaaaa tattcttaaa agttaaacaa
       61 atcattaaaa ttctctacca ttgttgggtg agtgaaaatt tgtttttgga aataagtata
      121 tgggatttta ttatccattg ccattgtgat gatattaatt aattcatggg attctgcacc
      181 aaataagcga gctcccaaaa taagatttgt ctcagtatct accacaactt taaaaatacc
      241 tctaaggtca ttattaacat gtgcacgtgg catggcagaa acaagaaggc tattagattt
      301 aacttgatat cctttttcct tagcggtctt ttcatctaga cctaccgttg ctaaaggagg
      361 atttgtaaag gttgaagtcg gtactgctcc tcgatttttt agagaataat ccttatcacc
      421 attcaaataa tttaagacaa tacgtgagtc atctaaagaa atatatgtga attgagggcc
      481 gccattaaca tcaccaactg caaaaatatt ctcaacagaa gtttggcaat attcatcaac
      541 agcaatagct ccacgttctg tcaatttaat atctgtattt tcaagtgcta aaccttctgt
      601 atttggctta cgtcctgttg catataatac tgcatcaaag cttaattttt catcctcaaa
      661 gctaataaca acgttttcat cttcattttg cacagatttg atatcggctg ataatttaaa
      721 agatatcccc atctcttcta aataatcttg agccatctct gataattcct cttcctcacg
      781 cgcaaagata cgagattgac tatcaataac tgtcactttg ctccctaatt cggaatataa
      841 agtcgcaaat tcaagcccaa tattaccgcc tccaatgata cctaaccgtt taggaagatg
      901 agccaattct tggattgccg ttgaatcata aacatgttga gaatcagcta atcctggaat
      961 tggtaattgt actgacttag caccagtatt gataataata acatcagctg taagttcttg
     1021 tttgtcagct cctcctgtta attcgaccac tttatttgat ataaagcgag ctttagcatt
     1081 ataaacatcc acagtgtctt tattatccaa catagcaaaa tttttagcac gtaaacgact
     1141 tgtaacttca tttctagtag tcatagcttc ttgaaaatcg tgatttttgc tagctgatac
     1201 taaaagcgtt tttgtcggaa tacatccaat gttgatacat gttcccccat acattttatc
     1261 gtcttcttcg accattgcaa cgcttttacc ttgtgttgct aattttgctg ctaatgtttt
     1321 acctgcttta ccaaaaccaa gaacaatgac atcatatttt ttcattttaa atttcctccg
     1381 cttttataat atagtttcag tatagcaaaa gatgtttcct ctgtctgaat tccagcataa
     1441 ggcatgataa gcctaactgt tacctttaaa tctagttttc taaaataaaa aaacccatcg
     1501 ctaaggacag gattttttct atttaacttc caccatacga ccagtatcta catcataaat
     1561 agcaccagaa ataacaacat catttgggat taatggagaa gcatgtaatt ttgccacatc
     1621 ttcacgaacg ctttcttcaa tatcattaaa aggaagaaaa tcatgtccat gcatatcaac
     1681 acccaaatct ctctgtaatt gagcagcaaa agcttcgtta gtaaacgtct gtgctccaca
     1741 atctgtatgg tgtagaacaa cgatttctcg tgttcctaat tgttgttgcg aaataactaa
     1801 agaacgtaga acatcggctg taactcgtcc accagcatta cgtaaaatat gagcatctcc
     1861 taaagctagc cctagagctt gagcaacgtg taaacgagaa tccatacagg ttacaatagc
     1921 aacctttgtc tttggcttga taggtagatg agctgttcca tgtaagtcag cataggcttg
     1981 attggttttt aaaaagttat caaaataagt ggtcatagag atctcctttg ttagtgtaac
     2041 atcattcgat atttactaaa taccaaccct tttatattat cattttgccc attatttttc
     2101 acgaaatgtc tctcgccacc aaaaaagatt cggctgaacc gaatctaatc attatgttga
     2161 aaacactgct tttaagacct gactaacagt tgtcacacca ataacatcaa tgcctttagg
     2221 tatttcaatc ccagctagag aattctttgg tgcgtaaatt ttagtaaagc ccaatttact
     2281 agcctcatta atacgttgct caatacgtgt cacgcgacga atctcaccag tgagtccaat
     2341 ttctccaata aatgattctt gtggatttgt gggtttttct ttgtaactgg aagcaatagc
     2401 tacagcaaca gctaaatcaa ttgctggttc atccaattta acaccacccg ctgattttaa
     2461 ataggcatct tgattttgaa gcaataaacc acaacgtttt tctagaactg ccataataag
     2521 actgacacga ttaaagtcaa gacctgtggt tgtgcgctta gcatttccaa aaacagttgg
     2581 agtcactaaa gcttgaacct ctgctaaaat aggccttgtt ccttccatag taacaacaat
     2641 agcagatcct gttgcaccgt ctaaacgttc ttcaagaaaa acttgacttg gattcaaaac
     2701 ttcaactaga ccacccgact gcatttcaaa gatacctatt tcattggtcg aaccaaaacg
     2761 gtttttgact gcccgtaaaa tacggaacgt gtgatgacgc tccccttcaa aataaagaac
     2821 cgtatccacc atatgttcta gcattcgagg gcctgctaat gttccctcct tagtcacatg
     2881 acctacaata aaagtagcaa tattattagt tttagctaac tgcatcaact cagcggtcac
     2941 ttcgcgaact tggctaacag agccttggac acttgatact tcgggactca taatagtttg
     3001 tatagaatca ataataagaa aatctggttt aatcttctca atctccgacc gaatactttg
     3061 catattagtc tcggcataaa ggtaaaattc attatcgata tcgcctagac gctcacttcg
     3121 taacttaatc tgttctgcag attcctcacc tgagacatat aatactgtac ctttattagc
     3181 tagctgagtt gatacttgta gtaataaagt agatttacct atcccagggt ctcctccaat
     3241 cagaaccaaa cttcctggta caacaccgcc tcctagaaca cggttaaatt catccatatc
     3301 agtcttagtt ctagaatagt taattgagga aacatctttt aacttcgtcg gtcgggactt
     3361 ttcaccgttc aaactaactc gtgcattttt aacttcttga acttcaacct cttctacaaa
     3421 tgaagaccag gctgagcaat ttggacaacg tcccaaatac ttaggtgatt ggtaaccaca
     3481 ctcttgacag gtgaagactg attttttctt agccactcag gcctccttta taacttctaa
     3541 attaatatga tagcttggta ccaaataact agtacactca ttatatcaca ccaagttaga
     3601 gaaaatataa gtttactctc atcaaaaaag gctaagaagt aagtctagcc ttttctaaca
     3661 ttattcgtaa aataatttta ttttccagta ctaccaaaac cacctgtacg cattcctgta
     3721 gcctgatcgt catctgccaa taagaatggg gcaaaaacag cctgaacaat ccgttcccct
     3781 tcttctaaaa taacagcctg atcagtaata ttttgcatct gtgcaaaaat atgtccttca
     3841 ttgacttggt tattatagta gtcaccatca ataacaccaa ctgaatttat taggacaatc
     3901 ccttttttac gtggattcga tgaacgatca taaaggtaca ggacctcccc tggttgcatg
     3961 tgtgctttaa taccggtcgg gaccaatgta atttcaccag gttcaataac cgttttttta
     4021 gctactttta aatcatagcc agctgcgtga gctgtttctc gttttggtaa taactcttta
     4081 ttgctaaatt ggctaactaa ttcaaagcca cgtacttttg acatgtgata ctccatcttt
     4141 atcttaaata tgcaaaaagt cgagttccaa atgaactcga ctagtctgcg gagagaggga
     4201 cttgaaccct cacgaccaaa agcggtcaca agatccttag tcttgcgcgt ctgccaattc
     4261 cgccatcccc gcttaacaac ataataaatt atatcaactt atcatattgt tgtcaatatt
     4321 atttttcata ttttttcaaa aaactagaga tacgtttctc atatttttct ggctctgttt
     4381 caaaagattt cgcatgtttt gcccctttta caatataaag ctctttctta cctgctgtag
     4441 ctttatagtt gtcataaacc atacttgttg gaacaaaatt atccttatca ccatgaataa
     4501 agagggctgg taaattattc tttttcaatt gttcgacact acttgcttgt ccatacgaaa
     4561 aacctgctct gattttagaa attgttgaaa cttcatataa gagtgggaag gctggtaaac
     4621 catacatctc tttagcctga aattttaatt catcccaaac actagaataa ccacaatctt
     4681 caatgatatt aacaacctga ctaggtaatt tttcaccact agccatcatg actgttgctc
     4741 cacccattga aacaccaaat aaagtaattt ggcttgatgg attcttatcc actatcattt
     4801 ctgtccattt gataatgttc tcgcggtcgt tccagccata gcctatcaac tgcccatgac
     4861 tttcaccatg tgcaatgttg tcaggcataa gaacattgta tcctaactta tgaaacagcc
     4921 aaccatatgc cttcatattc tctttgctat tcgcaaaacc atgaacgaca acaactgtct
     4981 tatgagtttt cttagcagca ggaacatacc aagcaacttg ctttatattt tggtttgtca
     5041 tttctatttt ttgctttaat agcttatcaa aggatttatc ataagcatat aaagagtttc
     5101 caggcttacg ttgaccattt gaaataaagg atttatcatc tcgaacttgg gcaacgtgga
     5161 aaaaataaaa actagctgct acactaatta aaaacaaaat aacaacaatc attttaataa
     5221 actttgataa tcgaatcttt ttcatgcaac ctattatacc ttatttttag aaaaagaaaa
     5281 gagctaatga tactttgata gtgaaactat ctaaaaaaat aaaatcgcct cctaatactg
     5341 ggaggcgata aagttatttc atattttcaa ctgctgctgt tacaaaagct gtgtaaagct
     5401 cttcagcatg gttaggacgt gattgtagtt ctggatggta ttgtgctgca acaaagaatt
     5461 ttttctctgg taactcaaca acttccatca aacgattatc cggtgatacc cctgagaaga
     5521 caaatccagc agcctcaaat tgctcacgga attttgtatt aaattcataa cggtgacggt
     5581 gacgacgttg gacaacttct tgattattat aagctgccgc agctcttgaa cctgctttca
     5641 atttacatgg atatagacca aggcgaagtg taccacccat atcttcaata tcgatttgat
     5701 cacgcatgat atcaatgatt gggaacggtg ttttagggtc aagctctgct gaattagctc
     5761 catgaaggtt taaaacatta cgcgcaaatt cgacacaagt tagctgcatt ccgagacaaa
     5821 caccaagcat tggaacatca ttttcacgtg catagcgaat tgcttcaatt ttaccctcac
     5881 tacctctttg gccaaatcca ccaggaacga tgataccatc tgcatctccg acaagttctt
     5941 taatattatc ttctgtcact tcagcagcat ttacccattt taggtcaata gcaacatcgt
     6001 tgacgtaccc tgagtgctta agggcctcca caactgaaag gtaagcatca ggtaattcaa
     6061 catatttacc aaccaatgca attttaactt ttttctcaag gttcattact ttatcaacca
     6121 ttgctgacca ttctgtcata tcagcagcag gtgtctcaag cttcaagtga tcacaaacaa
     6181 tttgatccat attttgagct tgcatgttaa gtggaatttg ataaatatgg tcaacatcaa
     6241 gggactctat aacagcctct ggtgcaacat cacaaaattg tgccaattta ttcttaatac
     6301 tttgacctgc tggttgctct gtacggataa caagcatatt aggttggata cctaggccac
     6361 gtaactcttt aacagaatgt tgagtcggtt ttgttttcat ttcgccggca gcctttaggt
     6421 atggtaataa cgttgtatga atgtacataa cattatcaga accaacatct gccttcattt
     6481 gacgaagtgc ttctaaaaac ggaaggctct caatatctcc aacagtaccg ccaacttcag
     6541 taataataac atctgaatct gtagttgtcg cagcacgttt gattttttct ttcaaagcat
     6601 ccgtaacgtg tgggataact tgaacagttg caccaaggta ttctccacgg cgttcttttt
     6661 taagaacttc actataaatc ttacccgttg taacatttga atacttgttt aagttaatat
     6721 caataaaacg ctcgtagtgt ccaaggtcaa ggtcagtttc tgcaccatca tctgtaacat
     6781 aaacttcccc atgttgataa gggctcattg tccctgggtc aatattaata tatggatcaa
     6841 atttttgaat tgtcaccttt agcccacggt tttttagtaa gcgcccaaga cttgctgcaa
     6901 caatcccctt accaatagaa gagaccacac cacctgtaac aaaaatatac tttgtcatta
     6961 aaaactcctt aaattaaatg ttaatctaac agttagttta cgggtaaaac ccaaaacaca
     7021 tgattgataa ataataccag cttctctagt caaaactagt agaaactatc tgatcaaggg
     7081 cctactcaga aaactatctg gaaccattct gtatctttcc caaaaacaaa atagccccct
     7141 atcactaggg agctttatct gacctcataa gaggtgcccg aataatagtt taaactatat
     7201 ctgactattt gtcaaataca aaatccccat ctaaacctct tcttcctctt cttcctcttc
     7261 ttcctcttct tcgttgattt ctacatcctc atctaaatct tcatcaggaa tgatttcatt
     7321 aatctcagaa tcatacgatt ctacctctga tttttcatcg tcagggtttt cttcatcata
     7381 ttctaaactt ggtgtttctt ctgtgaaatc ttcatcttct ggatcatcat cattgtaatc
     7441 aatagcatct tcatcaccat ccatgaaagc attgacacgt ttcttcttac gttttggagc
     7501 gccatcttca tcttcttcaa gagtgatgat ttcctcgtca atttcatcga tagcatacca
     7561 tgaacgaagt ccccatttgt tttcgccaag tggaataaaa cttccgtctg tattcaaatc
     7621 tgaatagaaa aatggcaatg actcacggat tgctgagtca gattttccaa ggtaggtttg
     7681 aatatcattt accaaatcgc tgaagtacat ttcgttatca cgaccacgtt gctctaaaat
     7741 agcgcgagca acttcaatca tcgaaagttc acttttttct tgcccagcaa atacttctaa
     7801 ttccaaagtt attctcctta attgtgtaag actatcattt tgtttagcaa tattctttat
     7861 agatagaaaa tagataagtc atattttcaa attttcgcta tttactattt tacgctattt
     7921 ttagctattc gtcaaaagaa taatcacaaa taaaaagact gggaagccca atcttttcaa
     7981 aatttaataa gcaattattt tacagtagct gagctagtga taacgtcaac cgcttttttc
     8041 attgcaatat catgtttaag catatctgct gaaagtagac cgcgtacttg gtcagcttcc
     8101 atattatatt ctgaagcaag atctgtgatt tctttttcga tttcttcatc tgttgcttca
     8161 aagccttctg cagctgcaat tgcttcaata acaaggttag ttttaacgcg tttgtcagca
     8221 tctgcttggt attgcttatg aaggtcttct tcagttgtac ctgttaattg gaagtacatt
     8281 tctggtgaaa ttccttgacg ttgcatatta cccatgaact cattcatagc acgatgtact
     8341 tcatcatgga ccatttcttc tggtaattca acaatttctg cattggcaac agctaattcg
     8401 attgctacac cttcaactgc atcatcaaat gcaatttctt ttgctgattc tagctctttg
     8461 cgatatttcg ctttcaactc atcaagtgtt tcaacttcat catcaatatc ttttgcaagt
     8521 tcatcatcaa gtgctggaac ttctttagtt tttacttcat gaattgttgt aacaaattta
     8581 gcatctttac cagcaagatc ttctgcttga taatcttctg ggaatgttac attaacgtct
     8641 acagtttgac cagctttaga acctactaat tgttcttcaa atcctgggat aaattgacct
     8701 gaaccaagtt caagtgagaa gttatctcct ttaccaccat caaattcaac accatcaact
     8761 gaacctacaa agtcaataac aactgtatca ccttgtgctg cttcaccatc tttaacagtc
     8821 aattctgcaa ggttgttacg ctcacgttca actttagcat caacttcttc atctgaaact
     8881 tcttttgaag catcaacttc tactgaaagg tctttgtaat ccccaagttt aacttctggt
     8941 tttgtaacaa cttcagctgt caacttccaa tcttgacctt tttccattga tacaacatca
     9001 atttttggtt gagcaacaac gtctaaacca agttctgcaa cagctgcttc gtaagccttt
     9061 ggcaacacta aattcaatgc attttcgtag agtgcttctt caccaaattt ttggttgaaa
     9121 actgtacgag gcatatgtcc tttacggaat cctggaacgt tcaaatcttt tttaactttg
     9181 ttgaatgctt gatcaagagc tggcttgatc tcatcttgac taattgtaaa tgtgataata
     9241 ccacggtttg tagctttatt ttcaaatgat gtagacatta agtcatttct ccttaaaatt
     9301 atttagatac agttta
//
LOCUS       DATRSG010000071         9221 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRSG010000071 DATRSG010000000
VERSION     DATRSG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.071"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..782)
                     /locus_tag="VBD89_001737"
     CDS             complement(<1..782)
                     /locus_tag="VBD89_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO7136246.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADF"
     gene            complement(782..2125)
                     /locus_tag="VBD89_001738"
     CDS             complement(782..2125)
                     /locus_tag="VBD89_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO7136247.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2265..3116
                     /locus_tag="VBD89_001739"
     CDS             2265..3116
                     /locus_tag="VBD89_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO7136248.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3119..4078
                     /locus_tag="VBD89_001740"
     CDS             3119..4078
                     /locus_tag="VBD89_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO7136249.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTQKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4132..5484
                     /locus_tag="VBD89_001741"
     CDS             4132..5484
                     /locus_tag="VBD89_001741"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO7136250.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5607..5978
                     /locus_tag="VBD89_001742"
     CDS             5607..5978
                     /locus_tag="VBD89_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO7136251.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6003..6383
                     /locus_tag="VBD89_001743"
     CDS             6003..6383
                     /locus_tag="VBD89_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136252.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6476..7606
                     /locus_tag="VBD89_001744"
     CDS             6476..7606
                     /locus_tag="VBD89_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO7136253.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7610..8347
                     /locus_tag="VBD89_001745"
     CDS             7610..8347
                     /locus_tag="VBD89_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO7136254.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            8348..9118
                     /locus_tag="VBD89_001746"
     CDS             8348..9118
                     /locus_tag="VBD89_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO7136255.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            9108..>9221
                     /locus_tag="VBD89_001747"
     CDS             9108..>9221
                     /locus_tag="VBD89_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO7136256.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLK"
BASE COUNT         3043 a         1431 c         1826 g         2921 t
ORIGIN      
        1 tcaaaatctg cttttgattt aacgtctccg tattcctcag gaatctctaa agataaaatt
       61 tcttcataac tagggaggac aatctcttta ccatatttgt tacggagggc agttgccaca
      121 agacggtaag ctagattagc attacgtgaa aggattggac cgtgaaagta tgacccaaaa
      181 acattcttat aatgaacccc ttcagtacca tcttctttgt tattcccatt tccataaatg
      241 acagttccta aaggtttttc gtcttctgat aaaaaggtac gtccttgatg attctcaaag
      301 ccataatatg tttcattgaa ttcatcgttg tgaatcttga tatcaccaat atagcgatta
      361 ttattttggt ttagcgtata gtgtcccata acgccaatgc cctctattcg ttctccatta
      421 gcttgaatat agtattgtcc taaaagttgg aaacctccac aaatagcaag tactacacca
      481 ttattttgaa tgaatttgtt aatgtcttct ttcttactgg gaagatcgcg tgcaacaata
      541 gcttgttcgt agtcttgtcc accaccaaaa aatgccatct gataatagtt gggatcaaat
      601 ctatcttcta atgaaacgat atcaaatgtc atttgacatc ctagtttttc tcccacatat
      661 ttcatcatta agatattccc attatcgcca taagtattta ataggttacc ataaagatga
      721 gctacattaa gagtgtattt ataatctttg gtagtaggag attttagtga ggtataagtc
      781 attaattcat ctcctttctg atagcgttgt ggttagcaag aatttcacga aactccagca
      841 tagctgtata tgttgctaaa atataagcat gctcagtctc ttgctgctca atcattgcca
      901 taatatcttg cagtttatca gcttgtttta tgcgtttctc atcataacca gttacacgta
      961 agcgacgagc aatttcagag tggcgaacac caccagcaaa tatttctggg atattcatcg
     1021 tgagaatagt ttcaaaatta gcgtcccaaa tccaagaagt atcaataccg tcagcataat
     1081 tggcatttaa tagtactgaa agagagaacg ggtatggagc aagtttaatc atatcaagag
     1141 cttgactagc tcctactgga tttttaatca gaactaaggt acatttttta tttccaattg
     1201 taaaggtttc ttgacgacca aaaacggcac ggcttttatc aaatccatct ttaatttgac
     1261 ttgcttctac accaaaatat tcagcaacag atactgccgc caaagcattg taaatattat
     1321 aaaggccacc cacgttgata ttatattgtt ggccatcaat aacgaaacct gatgaagtat
     1381 ttgtcagatg tattagcctt gttaaggcat aatcaaggtt tggacgttca aaatcacaat
     1441 taggacaagt gtagtcacca aggttggcat atgtgttcaa tcgataagtt aaaattgctt
     1501 gacactttgg acagagaata ccctcagtat tatagtgtgc taatcgtggc tcatgtttgt
     1561 cggtatcaaa tccatagaat tgcacagggt tagtcacgct tttagaatta aaaagtggac
     1621 tgtcaccgtt agctaagata gtagcttgcg gagcgttagc agctccatct aaaatcattt
     1681 gataagttgt gtagatttcg ccataacggt ccatttgatc acggaaaata ttggtaaaga
     1741 cgaacaaact aggctttata tactgtgtaa tacggggtaa actcgcttcg tctatttcaa
     1801 ggacagctat ttttttacca gatttacttt tcttagctgt taggaaggtt gaaacaatac
     1861 ctgtaatcat atttgctcca cttgggttgg ttaccacctg accgaaggct tcctttaaaa
     1921 taccaactgt taaagctgtt gttaaggttt taccgttagt accagtcaca actacaattt
     1981 cataatcttt ggcaattgta tctagtatat ctttatcgaa tttgagtgct aaactacccg
     2041 gataagtaga accacgtccc attttactta aaaggtaatg agcagactta ccggcagcca
     2101 ctcctagtgc tgtatttatt ttcataaaga tattttatca taaaaagatg ctttcttgct
     2161 aaagaaaata ttatgaaaac atggtataat gtagacgatg gaaaataaga aatcaaggag
     2221 ttaagcaaga aactcttcga caggctacca gtagagagga agatatggat attttttcag
     2281 ctattgattc aaagttttgg gcgagcatta tggaaaatcc atggatgatc cttattcatc
     2341 tcatggatat tatcatagta gctgttctga tttataaatt tatcaaagcg ctagcaggca
     2401 caaaaattat gtccttaatt cagggcgtta ttttgtttat catcattaga tttgtgtcag
     2461 aatggatagg gctaacaacg attacatttt tgatgaatca gattgttact tatggtgtaa
     2521 ttgcaggtgt tgtcatcttt gctccagaga ttagaacagg tctcgaaaaa tttgggcgaa
     2581 caccacaact atttacgcaa cgttctcaat taagcagtga tgaaaagcta gtagatgccc
     2641 ttgttaaggc agttgcttat atgagtccgc gtaaaatagg agccttgatt tcaattgaac
     2701 gtacccaaac tttgcaagag tatatagcta caggtatccc attagatgca gatatttcca
     2761 gtgaattgtt aattaatatt ttcattccta atacaccact gcacgatggt gctgttattg
     2821 taaaagataa aaaaattgct acagcatgtt cgtacttacc tctatcagaa tcatcttcga
     2881 tttcaaaaga atttggaaca cgccacagag cagcgattgg tctatcagag aattcagatg
     2941 cactaacggt tattgtttct gaagagactg gtggtatttc tgttgctttg aaaggtgaat
     3001 ttttacatga tctttctaaa gatagctttg aagcaattct gagaactcag ttgattcaaa
     3061 atcaagagga aaactcaaaa ttagcttggt ataatcaact attgaggagg aagtagacat
     3121 gaaaaaattt tttacaaata aattttggtt aggcgttgtc tcactctttc tggctatttt
     3181 attattttta acggcaactg caacaagtat gaaccatcaa gacaatagta agattgcagg
     3241 agcgtctgag acatatacac ataccctaac agatgtgcca atagacatca aatacgatag
     3301 tgatgattac tttattagtg gctattctta tggggcagat gtctatatgt catcggttaa
     3361 tcgtgttaag ctagattctg aaataaatga ggatacacga aaatttaaag tagtcgctga
     3421 tttgacaaat atgaaaccag ggactcagaa agtcccacta aaggtagtaa atttgccatc
     3481 tggtgtaaat gctacggttt ctccaacaac aattacagta actatgggta aaaagaaaac
     3541 aaaagagttt ccggtttatg gacatgttaa tgataagcaa attaaagctg gttatgcagt
     3601 cgataagatg tcagttgatg tgtcaaaagt taaagttact agtgatgaat ctattataga
     3661 tcgtattgac catgtcgctg ccaatatccc tgatgataaa gttcttgatg atgattttaa
     3721 caagacagtt actttacaag ctgtaacagc agatggtaca gtattagcaa gtattattca
     3781 cccttctaaa gcaactttat ctgtcaaggt aaaaaaattg accaagacag taccgattaa
     3841 tttgatacca gtaggacaat tcagcgatag tatttcaaaa attaactaca agttgagtca
     3901 agaaaaagca gtaatttcag gaacaaaaga ggctttagaa gctatttctg ttattaatgc
     3961 tgaggtagat atttcagacg taactaaaaa tacagaaaag aaaattaatc tttctgcaaa
     4021 caatgtttca gttgatcctg ctcaagtaac agttcaattg acaactacta aaaaataata
     4081 gataataaaa acgaaaaaaa tggaggtagc gtttttcgct agaaaaaaat tatgggaaaa
     4141 tattttggta cagatggtgt tcgcggtgaa gctaatgttg aactgacgcc agaattggct
     4201 tttaaattag gacgttttgg aggatatgtt ctaagtcaac acgaaactga tcgtccgaga
     4261 gtttttgtag ctcgtgatac tcgtatttct ggtgaaatgc tggaatctgc tttgattgct
     4321 ggtctacttt cagtaggtat tgaggtttat aaacttggtg ttttagccac tcctggagtg
     4381 tcttaccttg ttcgtacaga aaaagctagt gcaggggtta tgatttcagc aagccacaat
     4441 ccagcacttg ataatggtat taagttcttt ggtagtgatg gttttaaact tgatgatgat
     4501 cgtgaattag aaattgaagc tttacttgat gcgaaggaag acactttacc acgtccatca
     4561 gcacaaggtt taggtacttt agtagattat cctgaaggac ttcggaaata tgagaagttt
     4621 atggaatcaa ctggtattga tttagaaggt atgaatgttg cgttggatac agcaaatggt
     4681 gcagcaacag cctcagctcg gaatatcttt ttagacttga atgctgatat aagtgttatt
     4741 ggtgatcaac cagatggttt gaatatcaat gatggtgttg gttcgacaca tcctgagcaa
     4801 ttacaaagtt tagttagaga aaatggttcc gacatcggtc tagcttttga tggtgatagt
     4861 gatcgtttga tcgctgttga tgaaaatgga gagattgtag atggtgataa aattatgttt
     4921 atcattggga aatatctctc tgataaaggg cagcttgctc agaatacaat cgtaacaacg
     4981 gttatgtcaa atctcggctt tcataaagcg ctcgaccgtg aaggaattca taaagcgatt
     5041 accgctgtag gagaccgcta cgtggttgaa gaaatgcgta aatcaggata taatcttggc
     5101 ggagagcaat caggccacgt tattatcatg gattacaata ctacagggga tggacaatta
     5161 acagccatcc aattgactaa ggtcatgaaa gaaactggta agaaattatc agagttagct
     5221 agtgaagtga cgatttatcc tcaaaaatta gtaaatattc gtgttgaaaa taacatgaaa
     5281 gataaagcga tggaagttcc tgcaattgcg gaaattattg ctaaaatgga agaagagatg
     5341 gatggcaatg gtcgtattct agtacgtcct agtggtactg agcctcttct tagagttatg
     5401 gcagaagcgc caacaaatga agcggttgat tattatgttg atactatcgc agatgtcgtt
     5461 cgtacagaaa ttggtttaga ttaaaacggc gttattctgc tataaagtcg tcaatgttat
     5521 aaaataataa agagagggaa ttggagtgga gcatcaagca tacgcctttc tctctctttt
     5581 tgatttgaga agggagttta gagaaaatga ctaaagaagc agaattatta tgggctaaac
     5641 acaagtattt agtcttaagt aaatctcaga aaatttattt ggatattaga caaactttga
     5701 aatcaccaaa ttgtaccgtt ttagacgttc agtcactcat tgaccaagca gtgcttttag
     5761 aagaatcacc atcacaagtg actaatgctt atatgcatat atggggctat tttaaaaata
     5821 aggcagagcg tcaagaaaaa gaagaatttt taaccttgct ggaaaaatac aggaaaacag
     5881 gttatcaacg aagaaaactg ttagcttttc taaaacagct attagctaaa tacccaaatt
     5941 cttatttaca aaactcttct attttcgaag aagagtaaaa gattgatagt tgaggtatat
     6001 aaatgagatt atggcatcaa gatattattg agttattgcc taggcagcaa cttttaggac
     6061 agcatagaga gtgttgtgca ttgagaggga atgggtgggg gagaaaacat gagactgttg
     6121 attatgtttt tcgatattca ccttataggt tatttgcata tcatcaatta gttatggaag
     6181 aaatgatgga gcgtgggtat cgtgtatcta aagaatggct aattgcagaa tataggggaa
     6241 tgaagtgccc aagatatgat acacttaatc ctgttgactt ggagacacct atctatcctg
     6301 agcacaatca agactatcta caagaatgtc tatggaattt aaaagcaaaa ggaattgacc
     6361 ttcctataaa taagataaaa tagagattta tctctatttt tattttggaa taaaaagcta
     6421 tattatcaag ttttagagga gtgttttttt aagggcaaag tggtaaaata gaaacatgtt
     6481 aaagaaacca acctcagcat atgtacacat tccattttgt acccaaatat gttattattg
     6541 tgatttttca aaagttttta ttaaaaatca gcctgtagac gcttatcttc aggctcttat
     6601 ccgtgaattt cggtcttatg atattacaga attgcgcacc ttatatattg gaggagggac
     6661 acctacatct ataagcgctg tgcaactgga ctatttattg acggagttaa gtagagattt
     6721 aaacttaaat actttagagg agttcactat cgaagctaat ccaggagatt taaccgttga
     6781 taaaatagag gtgcttcaaa agtcagctgt caatagagtt tctctaggag ttcagacctt
     6841 taatgataaa catttaaagc gtattggacg cagtcacaat gaggtacaaa tttattctac
     6901 aattgatgct cttaaaacag ctggttttca aaatatctct attgacctaa tttatgcctt
     6961 acctggacaa acgatggatg atgttagaag taatgtagcc aaagctctct ctctaaatat
     7021 tcctcatttg agtctatata gtttaatttt agaacaccat actgtattta tgaataaaat
     7081 gcgtcgtggg aaattacatt tgccaacaga agatttagaa gccgagatgt ttgaatatat
     7141 catttctgaa atggagagaa atggttttga gcattatgaa atttctaatt ttacaaagcc
     7201 gggttttgaa agtcgtcata atttgatgta ctgggataat gtagaatatt atggtgttgg
     7261 tgcaggtgct tctggttatc ttgatggtat tcgttatcgt aatagagggc caatacaaca
     7321 ttatctaaag ggtgtttcag aaggtaatgc aaggttatct gaagaagtat tatcaaaaaa
     7381 tgaaatgatg gaagaggagc ttttcctcgg tcttcgtaaa aaaaagggtg tttccatcgg
     7441 aaaatttgaa caaaaatttg gaacgtcctt tgagaaacgt tacggtcaga ttgttcaaga
     7501 actccaaagt gatgggcttt tgaaagaaaa taatggtttt atccagatga ctaaaaaagg
     7561 tctcttctta ggagatacgg ttgccgagaa atttattgtg gagtaaaaaa tgggtttatt
     7621 atatcgagaa acttacgaag tgccttttta tgaatcagac actaatcatt atatgaaatt
     7681 accgcagttg ttggctttag cgttacaaat ttcagccaaa caatctttaa aattaggaat
     7741 aggcgatgac atcgttttta aaagatacgg attggtttgg gtcgttacag attatattat
     7801 tgatattgag cgtttaccca agcatgctga aaagatagtc attgagactg aagccaaagc
     7861 tcataataaa ttattgtgtt accgctattt ttatatttat ggtgaagacg ggcaaaaaat
     7921 aataacgatt tcatctgctt ttgtcttaat ggattttaaa acacggaaga ttcatcctgt
     7981 tcttgatgat attacttcaa tttaccaatc ccaaagaatc aaaaaagtta ttcgagggcc
     8041 gaaatatcat cccattggag atagtaaagt taaacaatac catgttcgtt attttgattt
     8101 agatatgaat ggacatgtta ataatagtaa gtatctagaa tggatgtatg atgtgttgga
     8161 cttagatttt ctaagtagcc acattcctaa aaaaattgat ttaaaatata ttaaggaaat
     8221 tcaatatgga acggatatta agtctcattg gtatcaagat ggtcttgtga cacgtcatga
     8281 tattattggt ggcgatgcta ttcatgctca agctaggatt gaatggcagg aaaaaaagga
     8341 ggattaaatg gcttataaag gatatcttat tgatttagat ggtactattt acaaagggaa
     8401 aagtcgtatc ccagcaggag agcgttttat cgaacgtttg caagaaaaag gcattcctta
     8461 tatgttagta actaataata cgactcgcac accagaaagc gttcaggaaa tgcttcgagg
     8521 cttcaatgtt gaaacaccac ttgaaacaat ctataccgca actatggcta cggttgacta
     8581 tatgaatgat atgaaccgtg gaaaaacagc ttacgttatt ggtgaagaag ggcttaaaaa
     8641 agctattgca gatgcaggtt atgtggagga tacaaaaaat ccagcctatg tggttgttgg
     8701 tttagattgg aatgttactt atgataaatt ggcaactgct acgttagcaa ttcaaaatgg
     8761 tgctttattt attggaacca atccagattt gaatattcct actgaacgtg gtttattacc
     8821 cggtgctggc tccttaaatg ctttattaga agccgcaacg cgtataaaac cagtctttat
     8881 tggaaagcca aatgccatca ttatgaataa ggcacttgaa attttaaata ttcctagaaa
     8941 tcaagcagtc atggtcggtg ataattattt gacagatatc atggctggta ttaataatga
     9001 tattgatact ttgttagtaa caacgggttt tacaacagtt gaagaagtac cagatttacc
     9061 aattcaacca agttatgttt tagcaagttt agatgaatgg acatttaatg aaggataagt
     9121 tattagtagt tttaacttgg atttggatta tatcgttagc aactctagct acaatttata
     9181 ttgcttggtt gatctatcct atagaaattc agtttttaaa a
//
LOCUS       DATRSG010000072         8789 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRSG010000072 DATRSG010000000
VERSION     DATRSG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.072"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            318..1388
                     /gene="xerS"
                     /locus_tag="VBD89_001748"
     CDS             318..1388
                     /gene="xerS"
                     /locus_tag="VBD89_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO7136257.1"
                     /translation="MKRELLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKE
                     YRRFLEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVS
                     QTTINRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGNETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEY
                     RGVPNRIDSSSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDKL"
     gene            complement(1431..2369)
                     /locus_tag="VBD89_001749"
     CDS             complement(1431..2369)
                     /locus_tag="VBD89_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7136258.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(2366..3034)
                     /locus_tag="VBD89_001750"
     CDS             complement(2366..3034)
                     /locus_tag="VBD89_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7136259.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            complement(3144..5099)
                     /locus_tag="VBD89_001751"
     CDS             complement(3144..5099)
                     /locus_tag="VBD89_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136260.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            complement(5101..5853)
                     /locus_tag="VBD89_001752"
     CDS             complement(5101..5853)
                     /locus_tag="VBD89_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136261.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(5879..6841)
                     /locus_tag="VBD89_001753"
     CDS             complement(5879..6841)
                     /locus_tag="VBD89_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO7136262.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            7007..7494
                     /locus_tag="VBD89_001754"
                     /pseudo
     CDS             7007..7494
                     /locus_tag="VBD89_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
     gene            complement(7526..8374)
                     /locus_tag="VBD89_001755"
     CDS             complement(7526..8374)
                     /locus_tag="VBD89_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7136263.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(8507..>8789)
                     /locus_tag="VBD89_001756"
     CDS             complement(8507..>8789)
                     /locus_tag="VBD89_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7136264.1"
                     /translation="LLNNNAKVYLAIKDNQIIGDVTAWDYGEYVEIDDFYVQESYRGQ
                     GIGTRLQFDASRGFKKVILISEEENRKMYEHQGYEEVAYYWTALRSPRK"
BASE COUNT         2935 a         1522 c         1330 g         3002 t
ORIGIN      
        1 tttaaagtag aatttaactt tggttattaa cttgcaaata taattaaaaa taattgagta
       61 cactttatta ccttttcaga taccgataaa atcaatgaaa ttatgaatca gttttctcaa
      121 aatactctgt ctttactata acaaacatgt taaacgataa taagtaatac tgatcatcat
      181 aaagaatgga tatttgatgt acttaccgaa tgaattcctg tttttaactt ttaaactttc
      241 cgaaaaacta taattttctt gaaagttatg atagagtatg atatacttct atttatagat
      301 ataatggaga ttttattatg aaacgtgaat tactactcga aaaaatcgat gaactaaaag
      361 aaattatgcc ttggtatgtt ctagaatatt atcaatcaaa gctttctgtt ccttatagtt
      421 tcacaacact ctacgaatat ttaaaagaat atagacgttt tcttgaatgg ttattggatt
      481 ctggtgttgc taattgtcat cacattgcag aaattgagct ttcagtttta gaaaacctta
      541 ctaaaaaaga tatggaagct tttatactat atttacgaga acgcccttta ttaaatgcta
      601 acacaaggca aaatggggtg tctcaaacca ccattaacag aactctatct gccttatcta
      661 gtcttttcaa ataccttact gaagaggttg agaatgctga cggggagcct tacttttatc
      721 gcaatgtcat gaaaaaagtc tcaaccaaaa agaaaaagga aactttagct tctcgggcag
      781 aaaatattaa acaaaagtta tttttgggca atgaaacgat agaattctta gagtatatag
      841 attgtgaata tcaaaacaaa ctttccaaac gtgctttagc ttttttcaac aaaaataaag
      901 agagagattt agctattatt gctttgctac tagcttcagg agtgcgtcta tctgaggcag
      961 taaacttaga tttaaaggat attaacctta atgtaatggt tattgatgtc acacgaaaag
     1021 gggggaaaag ggactctgtc aatgtcgcta gctttgcgaa gccctacctt gctaattatt
     1081 tagacatccg taaaaataga tacaaagcag aaaatcagga cattgctcta tttttatcag
     1141 aataccgtgg ggtgccaaat cgtattgatt cttcaagtgt agagaaaatg gttgctaagt
     1201 attcccagga ttttaaggtt cgtgtaacac ctcataaact aaggcatact cttgctacta
     1261 ggctatatga tgctacaaaa tctcaagtat tagttagcca ccaactaggt catgcttcca
     1321 ctcaagttac tgacctatat acccatattg ttaacgatga acaaaagaac gctttagata
     1381 aactatagta acataaataa agttatgtaa atattttatt ttacataact ttatttatgt
     1441 atagaaacag cagtcccata attaattttg ctatccaccg aaatggacaa attcagacta
     1501 gataagactt cttttgtcat atataacccc atgcctgttg ccttttggtg ctcatgaccg
     1561 ttgtaacccg taaagccatc ttcaaaaagt ctagggatat cctcttcgag tatgccgata
     1621 ccgtagtctt gtattctaat actctcttct cctatgctta ttattatctt tgactcagga
     1681 ttagaatatt ttatggcatt atctagcacc tgtgaaagag caaaagttaa ccactttttg
     1741 tctgttttcc agatattatc gccttcaatt atgatagata agcttttgga tagacaaata
     1801 accttatacg attttataat ttctactact acttctctaa ggctaacagc ttcaaaacga
     1861 aaatcgtcac gatattgtct aaatttcaaa aatgctaaca acgtttcaag ataatgttgc
     1921 aatttcaata attgttgttc aacttcctta ggatcgagat gatttgtctg ggccattaat
     1981 gaaatagctg ccaatggaac tttcatttgg tgcgaccaca tctttatcat aagtgcaaca
     2041 tgattggttt gttcaattgt ttcagaaact ttttgcgctt ctctagcttc taattgtgta
     2101 ataatattaa aataagcgta atcactcggt tgaagttcga aagaggggtc ctgactacta
     2161 ttaaaatatt tgagatgtaa catttttttc ctgtaacgac tgtattgcca aatactaatt
     2221 cctagtaaaa caataacatt taaacctaag gaattaaaca aatagggcat tggtaaatga
     2281 tatagataga aactaataaa aaataggaca aacatcatga tatataaaat ataccaaatc
     2341 aagtgttctc ttaaaaactg gcgtatcatt gtagtaaata cccaactcct ctcactgtat
     2401 gaatgtaatc aaaacctatt gggacaattt ttttacgtaa gcgtgtcata ttaacattaa
     2461 gtgtattttg atcaataaaa ctatcatttt cccaaagttt ctctaataga gactcttttg
     2521 agactacttg tttaggatgc atgagcaaga tagataggat tttattttct gttggcgata
     2581 aaataacctc tttatcttgg ctagacaata acccttctct tgtcaacgta aatcccccaa
     2641 aagttaattc ctgttggata aattgttgac ttctccttaa aatagcagtt agcttagcat
     2701 ctaatacagc tagagagaat ggttttgaaa taaagtcatc accccccata tttaatgcca
     2761 taaccatatc catttcatca ttactagatg aaatgaaaat aataggaatt gttaaaaact
     2821 tacgcaattc tgcagtccag taaaaaccat taaaataggg taacgtaata tccattagta
     2881 tcaaatcggg ttgaaatgcg ataatttctt gtttcacatc acgaaaattg ctgacactag
     2941 agacatgata gctagctgat aaatgatctt ttaaaagtga cacaatcgtc atatcatctt
     3001 ctacaatata aatttttcct tgctcttgtg acatatcccc tcctaaaata gtacgaaatc
     3061 tctttctttc atttagtata acaaattttt tagtctatcg gtttctatta atgcaaaagg
     3121 attggtaaaa taaccaatcc tttttagcgt tcaataatat gataataagt tcgactagtt
     3181 atacggtaaa tgataaagta aattatagaa atcgcaagta ctgtcaaacc acttactaca
     3241 tatacgattg ttgagttaag caccccaaat accaaaagca tctgttttag cattggaata
     3301 gcaactccaa agtgaataac tgctaccact aaaggttgga agaagaaaat cataatttga
     3361 gaattaatag tccgtttaac taattttttg ctcataccta cttcttgtaa gatacgataa
     3421 gatctcttat cttcatgacc ttctgagtat tgcttatagt aaacaattag agctatacct
     3481 agcaaaaagc ttatacctaa cagaaatcca gtaaataata acccaccaaa catttgataa
     3541 ccttctttta gaaaactctg tttagtgcta atatttccaa catacttacc atctacctct
     3601 attatgtcgt ttttagaaat tgaatttatc tcttgactat tcaaatccaa atatgcctta
     3661 aaggtttttg gaaaagtatt gatgttctta gtataaggta aatatgcttt tctaatattg
     3721 tcaatttgtt tgttgtttgc aaatataatc aaacccgggt tgtaagtatt tgtagtctct
     3781 gggacataag cctcttttaa attttttaca acgtcaaatt tatttcccaa tagatttatt
     3841 tttttcaaac ggctatcacc tttttgaaca aagtaagcca cttggttatc ttttaatttt
     3901 ggtaattggt gacctaaacg acggaaatca ttctgagtaa ttaggtacat aaaacccgtt
     3961 tttgtgatat caacatgttt gacgttttta gatgtgatat taagttcact tgattgacta
     4021 actggcatcg aaatcattgt ctgattataa gtgatagctt ccttagatga cttacctagt
     4081 ttcttcaaaa tcttttcttc aaatatattt ttcgcgtcac cttttgaatt atctattgat
     4141 aaacttactg attttggaaa tagtccggta acaacatttt gtgtatttga atagagtgag
     4201 actgttgtag caatagtaac tagagccata acagctaata aagtgatact tgctaaccct
     4261 actgcatttt gcttcattcg aaaaatcatt tgcgaagttg atacaaaatg ctcagattta
     4321 taataataat gcttgttttg acgcaacctt tttaagtacc atgtcataaa actaatataa
     4381 aaaagataag taccagcaat aactaaaagt actgcaaaga agaaacgata gataacagct
     4441 aatgcaggtg ctttacctga agtaagagcc attgtataag cgatggcgat agctactaac
     4501 gctaaaattg caagtataag attacctttt ggttcttttt ctccctgttg tttctttcta
     4561 aaaagactta atggggatga taaatgaaca tgtcgaataa ctggaacttc taaagtcaga
     4621 aaaatacctg taaatataac gatacaaata ataaatggcc ataaacttaa actaagattt
     4681 agtgcatgat agttaataat gttgacaaaa attaaataaa taaatttagc aaaaaaagca
     4741 ctaaacagac ttcctataga aataagaaat atataaataa taaacagctc tagactagct
     4801 acacgcgcaa cttgacgttt attcatcccc aaaatgttat acagtccaaa ttcactacta
     4861 cgctgtttca tcaagatatt gtagctataa tgttccatga ctaccgcaaa gattgacaaa
     4921 acaatcactc caagaaaaag aaccgttgcc gctgtcccca tatctcttcc aacagcactc
     4981 attaagatta gtagcgtaga acaagtcaat gaataaagta ataaactggc taagaggaat
     5041 ggtatgtact ggtctataga atgttttaaa ttatgccagg ctattttgag ataaaacata
     5101 ttattcaccc cctaatatgg cagacattgt taaagaaata tctttattga attcgctatt
     5161 attcttatta ccacgataaa gttggtgaaa aatacgtcca tctttgataa ataaaacgcg
     5221 tttggcatga ctagcagcat ttgctgaatg ggttaccatc aaaatagttt gtccatccaa
     5281 gttaatagtt tcaaataaat tcagcaagtc ttctgaatta cggtaatcta acgctgctgt
     5341 tggttcatct gctaataaaa tttgagggtt ggttatcaaa ctacgagcaa tcgctacacg
     5401 ttgcttttgc ccaccagaga gctcgaaagg tctcttatct aataagtcat caattctcag
     5461 atgagaagat aattctgaca aacgatgatc catttctttg taacgtttac gatcaagaac
     5521 taaaggaaga tagatattat ctcttacaga gagagtatcc aaaaggttga agtcctgaaa
     5581 gacgaaacca agatttttca aacgaaaact cgctaatttt gcctctttaa ttttcgtaat
     5641 atcttcccca tttaaaatca cttgtccatt tgtcggtttt tctaaagtag ctaaaatatt
     5701 tagcagagtt gtttttcccg aaccagactc tcccataata gcaatgaatt ccccttgttc
     5761 aactttaaaa tcgacgtctt gtagagccct tgtctcttct tttgaaaaac gtgtacgaaa
     5821 aactttttct aagtgattga tttctaataa catataattc tcctttattt attataccct
     5881 aattttgatt gattctactc tttaaccaag actgtgactc ttgtaaagga aggctggtta
     5941 cttgatctgg taagataggg gtgttctcgt atttatatcc ttgtcgatca ctaactattc
     6001 ctgtagttaa agctaatcta gcaccgtcgt aaagcatgaa agtttcatta acagtcgtat
     6061 atcctgctgt agcttgacca aagcttttaa cattaggtaa tcctttaaat gataaaaaag
     6121 tcatttctgc cgaactagca gttttatgat tagttataat agcgattgga acatgcttag
     6181 tattgattgt tttcctagat atttttaatg cttctagcgg tatatttttc atcgtgattg
     6241 ttttcttatt tccatactta tcagtataat gaaatagggt atcatttggt aaaatagagg
     6301 ctaacccccc aatcataggg atcatattgc cgccagtatt attcgataag tctaaaatga
     6361 gtcccttata acttttttcc atcaatatat tttgtaattt accactataa tggctaatag
     6421 atcgtttatc taacttactt atagaaggaa tagtaattaa gagaatgtct tcgtttaatc
     6481 ttcgatattc tggatatcga ctatctaggg tatctcttcg tacagtctct ttggataata
     6541 tttgactatg tttaccgcct ccataccgta ctgcttcctg tacaatgttg cgactctctt
     6601 gataagattt tgacgttttc aacttttttt ctgatcgttg tcttataatt tgccattgac
     6661 gaccttgcga atacaaacca cgctgttcaa cacgatctaa aataacacgt ccataacgtt
     6721 cgctactagg aggtaataga taaatattta atgctgaacc ataataatga actaccactc
     6781 ctaaaattcc taaaactata agcattggaa ctacaaaaag taaaactatt tttcgccgca
     6841 tctcttgctc cccctttctc tttaagtcta ttatatggca aaatggttta ctataacctt
     6901 acatcttaag acctatttct tacaaatttg aaagttttaa aagctttata aactataatt
     6961 ttgatataat atcttacata acaaatggaa agtacggtga aaacatatgg ccaagacaag
     7021 taaaaaattg acaaaatctt tagggcctat tggtaaatta atttcaataa tccctgacac
     7081 aactgaactt attggaaaag caattgataa ttctcgtcca attattgaaa aagaacttga
     7141 ccgtcgtcat gaaaaaaaca gacttatact cagcttgata atgtagtaca ccttgatatc
     7201 aatgatgcgc aagtttactt agagcaacgc cattttaatg tatcaaggat tgttgctgaa
     7261 ccaaattcaa agtgggcaac cctacatcct aatcaagttg tgaatatgtc gcctaaagct
     7321 ggtagggtca gaatcggttc attagtgaag ttgtactata ttacagagga tatccttgaa
     7381 gaaagtaaga cactactttt atctaaatcc gaaaaaagta aagcacgaca acaacggtta
     7441 acagaagtat tgagtacccc tagtcaatta ctcaaaaaaa atcgaaataa ataagcatga
     7501 tcgaattgac catgcttatt tatttttacc aaacaggttg atctaaacct tgagatgttt
     7561 tttcgataac ttttttaacc gcatctgtat ggtaagcttt cacaacttct ttaatggctt
     7621 tagcttttga tttgtcttgc caaccttttt gagcaactaa aaggttgtac cattgttttg
     7681 catttttact ttttggttca ataaaaatag ctgatttagg atttattcct gcttctgtca
     7741 caaaatcatt attaataact gctgcatctg tggaatccaa agaacgtgcg gtctgtgctg
     7801 catctacttc ttttaaatct aaagatttag gattggaaac aacatctgac attgttgcta
     7861 atgtatcacc tgatactttc aattttagca agcctgctga ttgtaacaag tagagagagc
     7921 gactttcgtt aactgcatcg tttgggatag taatagttgc tttatttgga atttcagaaa
     7981 ctgtttggta tttaccttta ccgttcttag taccagagta taatctaaag gaagtaaagt
     8041 atgtctcagc aacggaaaca agattggtct tatttgcttt attccaattg tttaagtaat
     8101 tataatgctg gaaagcatta atatcaatct catcactttc taaagcttta ttaggttgtg
     8161 tataatctgt gaattccgta aattttagct taacgccttt tttcttaact aattcttcga
     8221 ttttatccca acgagctttt tcagttttac tctttaccat tgttccaact ctaacgactt
     8281 tggcgtcttc atttttagac tgaccacagg ctcctaaaat aagtgctgaa attactactg
     8341 ttgtcgttaa ccctaatagc tttttgattt tcataattta tttctccttt gatatggttg
     8401 tatgattaat caactgatac tagtgtaaca aaaagctaat ggattaaaaa atacttatta
     8461 tcaatcaaga gatatagctt ttaactatat catttctttt ataattttac tttcgagggc
     8521 tccgtaaagc ggtccaataa taggcgactt cctcataacc ttggtgctca tacatttttc
     8581 tgttttcttc ttctgaaatt aagataactt tcttaaaccc cctacttgca tcaaattgta
     8641 ggcgcgtacc aattccttgt cctctgtagg actcttggac ataaaaatca tctatttcca
     8701 cgtattcacc atagtcccaa gctgttacat ctccaataat ttgattatct ttaatagcta
     8761 ggtaaacttt agcattatta tttaaaaga
//
LOCUS       DATRSG010000073         7645 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRSG010000073 DATRSG010000000
VERSION     DATRSG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.073"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            15..674
                     /locus_tag="VBD89_001757"
     CDS             15..674
                     /locus_tag="VBD89_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO7136265.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDI
                     FSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYR
                     F"
     gene            725..1402
                     /locus_tag="VBD89_001758"
     CDS             725..1402
                     /locus_tag="VBD89_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO7136266.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1395..2840
                     /locus_tag="VBD89_001759"
     CDS             1395..2840
                     /locus_tag="VBD89_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO7136267.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLDPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2847..5006
                     /locus_tag="VBD89_001760"
     CDS             2847..5006
                     /locus_tag="VBD89_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136268.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKESKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            5146..6558
                     /locus_tag="VBD89_001761"
                     /pseudo
     CDS             5146..6558
                     /locus_tag="VBD89_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            6798..7298
                     /locus_tag="VBD89_001762"
     CDS             6798..7298
                     /locus_tag="VBD89_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7136269.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAALGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
     gene            7496..>7645
                     /locus_tag="VBD89_001763"
     CDS             7496..>7645
                     /locus_tag="VBD89_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136270.1"
                     /translation="MEKITNDFEKVIAVLNRLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKI"
BASE COUNT         2647 a         1062 c         1482 g         2454 t
ORIGIN      
        1 gaaaaaaata tttaatatca cttgaagatt actatctttt atctaagaaa ttttcccaag
       61 taatgacgct cgattcgcat agttcagacg acggctatac tatccgttcg ctatactttg
      121 attctttaga agatgttgat tggcaagaaa aggaagatgg tgttgagata cgaagaaaga
      181 ttcgtttgcg caattatggt agtcaaagca attttgcgaa attagaaatg aaacaaaaac
      241 aaggtgctaa tcaaaaaaag cgctctctaa aattaagcaa gacagatgct caagcgctga
      301 ttaatggtaa caatagtgtt ttattaaatt atccggatac atttgctgcg gagtgttatt
      361 caaggataac aacgcaactt tatcaaccta aaacggttat tgagtaccaa agaaaagctt
      421 ttgttgctaa ggaaaacaat atccgaatca cctttgacca ccatataata ggtacagagg
      481 ctagttatga tattttctca gaggatttaa atcaaaatta tttattagat ccttatcttg
      541 ttattttaga agttaaatat aatggatttt tattgtctta tattaaagaa ctcttagatt
      601 cggtagatag aagtgaactt tcagcgagta agtattgttt aggaagagct gttagtaaac
      661 attatcgttt ttagtgaaaa agtatatacg ttaattatta ttaaatgtga gaggataaca
      721 aaaaatgaaa gacctattac gaaatagtct agagcaaagt ggaaatttaa gttttcaaga
      781 tatgatttta catattcttg tagcagcttt attgagtgta gttatttatg tttcctatgc
      841 ttatacgcat agtggaactg cctatagtaa aaagtttaat gtttcattaa tgacattgac
      901 ggtcttgact gcaacagtaa tgaccgttat tggtaataat gtagccttgt cattgggtat
      961 ggtcggtgcc ttgtcagttg ttcgttttag gacagccata aaagattcaa gagatacagt
     1021 ttatattttt tggaccatag ttgttggtat ctgttgtggt gtcggtgact atgtggtagc
     1081 tgcattagga agtagcgtta tctttatctt attatgggtt atgggacgtg ttaaaaacga
     1141 gaatcgtatg ttattgattg tgaagtgcga tagaacacta gaagttgatt tagaaggaat
     1201 tttcttccaa tattttgacg gaaaagctgt tcagcgtgtt aaaaattcaa caactaatac
     1261 tattgaaatg attttcgaaa tctctagaaa agattacgat aagcaactcc atgtagataa
     1321 tcagttaact gaaaaagtgt accaattggg aaatattgat tatttcaaca ttgttagcca
     1381 aagcgacgaa atcaatgggt agacttatga gagggaaggt tatttacggc acaaccctta
     1441 taggtctttt tctattctta tttttctatt tttggattcc taagcatcac atcgagagaa
     1501 tacatcatca tcgtataaag caggtagatg cgaagagtga tttaacagga tttaaaaccc
     1561 atttgcccat tatcagcatt gatacaaagc aacaagttat tcctcttgtt acaaaagaag
     1621 gcggaaaata tgtcaaagct agggataata ttaatgttga tatcgaatta cgggattctc
     1681 caagtagatc acatcattta tcagaaaagc cgagaattag gacaaaaggg ttaatatcat
     1741 atagaggaaa ttcctctcgt tactttgata agaagtcatt gaaagttaag tttgttacta
     1801 ataagttaaa ggaaaagaag catcgattag caggaatgcc taaagaatcg gagtgggtat
     1861 tgcatggtcc ctttctagac agaacattat taagaaatta tctgagttat aatattgctg
     1921 gtgagattat gtcctatgcc ccaaacgttc gctactgtga gttatttgtc aatggtgagt
     1981 atcagggagt atatctagca gttgagaata tcgaacaagg tgagcaacga gttcccattg
     2041 aaaaaagtga taaaaagttg cataaaacac cttatatcgt tgcttgggat cgtgaacata
     2101 aggcaaagca aaagttagat aactatgtac attatacaca ccagtctggt atctcagctc
     2161 ttgatgtgaa atatccaggt aaacaaagat tgacatcaaa acaattagag tttattaata
     2221 aggacatcaa tcatattgaa aaagtccttt attcttatga tttttcacaa tatcctaaat
     2281 atattgatag agaatcattt gcaaattatt ttgtcatcaa tgaatttttt agaaatgtgg
     2341 atgcgggtaa attttcaacc tatctatata aagatcttcg tgatagagca aaactagtag
     2401 tttgggattt taataatgct tttgataatc aaattgaagg acgtgttgat gaggcagatt
     2461 ttaccttgac ggatgcaccg tggttcaata tgctcattaa ggacaaagca tttatagacc
     2521 tggttgttca tcgctataaa gaattacgaa agggagttct agcgacagaa tatttatcta
     2581 attacataga cgagacaagg cattttttag atccagctat tgatcgaaat tataaaaaat
     2641 ggggctatgt ctttgattta aagaatactg atccaaggaa ttatttgatt cctactgaac
     2701 gcaatgtgac aagttatcac aaatcagtag aacaattaaa agattttatc aaaaagcgtg
     2761 gcagatggat ggatagaaat attgagacct tgtatcaata tagtgcacca tcgaaaaata
     2821 ctaatacttt actggagtag aacattatga aaaaatatct aagtattgta gcccttttaa
     2881 ctattttgtt tagctctatt tattatttgt attactttga tggtagtttg tatttaccaa
     2941 agggcttatt aaaagaaaat acaagaacta actttgttgt taaaggtgat actgtacttc
     3001 acaagcccac caataaacct tttgttgtta aaggagtaga cgttgagtct tccttagcgg
     3061 gttatcatca caacgatttt cctattactc aaaaaacgta tcgtgaatgg ttccatttaa
     3121 tttccaacat gggggcaaat actgtaagag tcaaggtacc gatgaatgtt gcattttacg
     3181 atgccttata tcaccacaac aaagaatcaa agaggccact gtatttgttg caaggaatac
     3241 gtatagattc ttatcgcaat aatgcttcta taacagcttt taatgataat tatagggggt
     3301 atttaaaacg agaagcaaaa ggcgttgtgg atattctcca tgggcgtaag caagtatgga
     3361 atactgattt tggtagccgt cattatcatt atgatcttag tccttgggta cttggttatg
     3421 tcgtagggga tgattggaat agtggtactg tcgcttatac taatcatcaa gagaaaaaaa
     3481 cgcaatataa aggacgttat tttaaaactt ctgtggcagc taatccattt gaggtcatgc
     3541 tagctcaagt aatggatgaa ttgacacatt atgagacagc taaatatggt tggcaacatt
     3601 tgattagttt ttcaaactca ccaacaacag acccttttca ttatcgaaaa ccatttgagg
     3661 cacaggctcc taaatacgta caactaaatg tagaaaatat tcaagctaat tcaaatgtta
     3721 aagcaggtat gtttgcagca tataaagcta ttgatttcca tcctcgatac aaggattatc
     3781 tattatttga taaagagaat atcagtaaag aagatagaca aaagattaaa gaactttctt
     3841 tgtcacaggg atacgttaaa ctgctaaatg cttatcacaa aatccctgtt ctagtcacgg
     3901 gttatggcta ttcgacagcg agaggtattg cccaaaaaga aattgataaa cgtcctctgc
     3961 cgattaatga aaaagaacaa ggtcagcgtt tactagaaga ttatgaatct tttatatcat
     4021 ccggtagttt tggagcgact atcaatgcat ggcaagacga ttggaatgca agggcgtgga
     4081 atacatcttt cgccacaaat aaacatagtc aattcctatg gggggatgca caagtattta
     4141 atcaaggtta tggtttatta ggctttaaaa acgcaaaaca tcattatcaa gttgatggta
     4201 aaagaggcaa aggagagtgg aaacatcctc tgatgactag tgcaacagga gatgacttat
     4261 atgctagcag tgatgaaagc tatctctacc ttgcgattaa aacaaaacct gaaaaactaa
     4321 aagaaaaacg attattacca atagatatta caccaaaatc tggtagtaga aaaatgaatg
     4381 gtagtaaggt cacattttct aaatctagtg actttgtatt gtctattgat ccaaatggca
     4441 agtctgaatt atttgtccaa gagcgctata atgccttaaa agcgaactat cttcgacagc
     4501 ttaacggtaa agatttttat gctttcccac caaagaagaa cagtagtaat tttgagcaga
     4561 taaatatggt attgagaaat acaaagattg ttgaagacat ggaaaaagta aaagcaacag
     4621 agaggttctt accaactcat cctactggtc ttctcaaaac aggaacaact gataggcacc
     4681 aaaaaacatt tgattcacaa acagatattt cgtttggaaa ggactttata gaggtcagaa
     4741 ttccgtggca gttgttgaat ttttctgatc catcatctca aaaaattcac gatgattact
     4801 ttaaacatta tggtgtgaag gagttagaaa ttgagagcat tgctttagga ttaggtgcta
     4861 atagcaaaga aaacacactg ataaagatgg cagattatcg tttgaaaaat tgggagagac
     4921 ccgataccaa aaccttttta aaagactcct attatagtat taagaaagaa tggtctaaag
     4981 aaagagagag aacatatggt acataatatt ttatattttt acattttgat ttgcttagca
     5041 attattgttt ttaacattca ctatatctta agacaacgat tagataagct tagttggctt
     5101 cgtattcgga aaaaggatat aaatatcaaa taaaggggat tttctatggc attatttttt
     5161 accttcctaa aggtatctca agtcattttc ttagtattat tgattgccta tgccagcttt
     5221 ttagtgattt cattagtcat tggaggtgtt catctctatg aaaaaaatga gcgcaaaaaa
     5281 agtttaacta gaaataaaga aaaaattcca atttcaatta ttgtaccagc ctataatgaa
     5341 tcaacaacca ttgtgagttc gattgattcg ttattgcacc ttgattatga agcgtatgaa
     5401 attatcgttg tggatgacgg ttccagtgac aatacttcag atgttctcaa ggaagagttt
     5461 gcattaatga aaatctcgaa tacgattgat tcaattattg ctactcaaac ttgtaaagat
     5521 gtctttcaga gacaggttgg taaggttaaa ctgacactca ttgttaaaga aaatggtggc
     5581 aaaggggatg ccttaaatat ggggatcaat gctgctaatt acgattattt tctatgtcta
     5641 gatgcagatt ctatgttgca ggtagattct ttgagtcaaa ttagcaaatc catacaggta
     5701 gatccgacgg ttatagcagt gggagggctt gtccaagttg cccaaggtgt taagattgag
     5761 caggggaaag tagcatctta tcgtttgcct tggagaatta ttccttgtgc acaagcactt
     5821 gagtatgatt catcattttt aggagctcga atctttttag actatttaag agctaattta
     5881 attatttcgg gggctttcgg tctttttaag aaagaccttg ttaaagcggt gggaggatat
     5941 gacacccaga ctctaggaga agatatggag ttggtgatga aattacattt cttctgtcgt
     6001 aataataata ttccttatcg tatttgttat gaaacagatg ctgtttgttg gagtcaagca
     6061 ccaacgaatt taggggattt acgtaaacag agacgccgat ggtatcttgg cttgtactaa
     6121 tgtttaaaaa aatataaaag tatttttgcc aattatcgct ttggagctgt aggatcaatt
     6181 tcttatattt actatatttt atttgaatta ctgacccctt ttatagaatg ttttggtatt
     6241 gtcattatat ttttgtcctt attatttaat caattaaaca tcccattctt tatatcattg
     6301 gtgagccttt atatctttta ctgtgtttta attacattaa gctctttctt acaccgtatt
     6361 tattctcagc aactagtaat aggaatttta gatattgtta aagtatttta tattgctgtt
     6421 tttaggtatc ttatcctaca tcctgtatta acttttgtta aggttgcaag tgttattggc
     6481 tataaaaata aaaaaatggt ttgggggcat attacgagag agaaacaaat gaaggatttt
     6541 gaaacaagtg ttacttagaa gttatagcga ttcatatata taaaataatc actaaaagag
     6601 cgtaattatc tacgctcttt tagtgattat caataattgg cctaatctga ggttaatgtt
     6661 taaaaccatc taaaatttta gaaatataat gataaatgag aaccagaact gttcaaaata
     6721 aaaattaata tcacgaaaat ggtataattg gagtagttat caggcagtta ataataattt
     6781 ttgaaaggat tttagttatg gcttgcataa tcaggagagc aagattagga gatgaagtca
     6841 atttagctta tattcaaacg gagagctgga aagctgcttt gggtaagata ctacctgagg
     6901 atatcattca aaaaactacg gaaatagaac ctgccataac aatgtatcaa cagctactac
     6961 acaaggaggt tggtaagggg tatatattgg aagtagatag caatcctcat tgtatggctt
     7021 ggtgggataa aagtagagaa gatggtatgc ttgattatgc agaactcatt tgtatacata
     7081 gtcttaaaga aggttgggga aaaggttatg ggtctcaaat gatgaatcat gtactatcag
     7141 agatacaaca agcaggttat aataaagtga tactttgggt atttacagaa aatacacgcg
     7201 caaggaaatt ttacgatagg tttggttttt cttttaaggg taaatcaaaa acttattttg
     7261 aaaaggaaga aatgtgttat gagaagattt tgcactagcg agataaagca tccgataata
     7321 caatagtata aacatcctat tttgaaaaac aatttccttt aaggggaaca aatttgtaaa
     7381 agaaaattgt tataggtata atgaagatta tgaaggtaaa attttttgag tgaaaaacag
     7441 tgtatcaatt ttaggttctc tagtctacaa atagttatgc taactctatg gaagaatgga
     7501 gaaaattacg aatgattttg aaaaagttat agctgtgcta aatcgactaa gtatttctta
     7561 tgaattagtg gaaaatggac ctgttttgtt gacggagcaa gcagataact ttattgtggg
     7621 aattgaagga gtaagaacaa aaata
//
LOCUS       DATRSG010000074         7622 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRSG010000074 DATRSG010000000
VERSION     DATRSG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.074"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..431
                     /locus_tag="VBD89_001764"
     CDS             <1..431
                     /locus_tag="VBD89_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO7136271.1"
                     /translation="LDKAKIEHDSLQLDALQGDLPNGIQKQDIFKTLALIGDKTGPII
                     GILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANNPIGIRQKHNYPIFID
                     TIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            453..1094
                     /locus_tag="VBD89_001765"
     CDS             453..1094
                     /locus_tag="VBD89_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7136272.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(1130..2032)
                     /locus_tag="VBD89_001766"
     CDS             complement(1130..2032)
                     /locus_tag="VBD89_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7136273.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMASERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            2207..3697
                     /gene="lysS"
                     /locus_tag="VBD89_001767"
     CDS             2207..3697
                     /gene="lysS"
                     /locus_tag="VBD89_001767"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO7136274.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3772..4242)
                     /locus_tag="VBD89_001768"
     CDS             complement(3772..4242)
                     /locus_tag="VBD89_001768"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO7136275.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(4257..5450)
                     /locus_tag="VBD89_001769"
     CDS             complement(4257..5450)
                     /locus_tag="VBD89_001769"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO7136276.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(5468..6118)
                     /locus_tag="VBD89_001770"
     CDS             complement(5468..6118)
                     /locus_tag="VBD89_001770"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO7136277.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(6099..7208)
                     /gene="ribD"
                     /locus_tag="VBD89_001771"
     CDS             complement(6099..7208)
                     /gene="ribD"
                     /locus_tag="VBD89_001771"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO7136278.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(7402..7516)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         2432 a         1575 c         1179 g         2436 t
ORIGIN      
        1 ttttagataa agcaaaaatc gaacatgata gtctacaatt ggatgcttta cagggagatt
       61 tacccaacgg tattcaaaaa caagatatct ttaagacatt agcgctaatc ggtgataaaa
      121 ctggtcccat aataggtatc ttacctctca ccgagcattt atccgaaaaa aagttagcta
      181 aaatttcagg aaacaaaaag gtacaaatga tacctcaaaa agatctccag aaaataacag
      241 gttatattca cggagcaaat aatccaattg gaattcgcca aaaacataat taccctattt
      301 ttatcgatac tatcgcttta gaaaaacaag aactcattgt ctctgcagga gaaattggac
      361 gttctattcg tattaatagt gaggttcttg cagatttcgt aaatgctaaa tttgccgata
      421 taaaggaata ataattagga ttggagaaaa atatgaaatt ttatcttgtt agacacggaa
      481 aaacacaatg gaatttagaa gggcgtttcc aaggtgctaa cggagactca cctctccttg
      541 aagaagctat tgaagaatta gaagaacttg gacaatacct atcatctatt cattttgatg
      601 ctgtttattc tagcgactta ggtcgtgcta gagatactgt taatatcctc aatgatgcta
      661 attcatgccc aaaagaaatc cactatactt ctcagcttcg tgaatgggca ttgggaactt
      721 tagagggttg taaaattgca actatgcaag ctatctatcc tcgtcaaatg accgcatttt
      781 atcaaaatcc cttacaattt aaacatgaca tgttcggtgc agaatctctt tatcagacaa
      841 cgcatcgtgt agagagtttc ttacgttctt tagcttcaaa aaattacgac aaggtcctta
      901 ttgtaggaca tggagcaaat ctaactgcgt ctattcgttc cttactgggt tatcaatatg
      961 gtagtttaca ctacaaagat aaacttgata atgccagcct cacaatcata gaaactcatg
     1021 attttaaaga cttcaactgt ctgacttgga acgataaatc ttatttaagg caagaggtca
     1081 aaatgacaca ctaaaataat ccactagatt catctagtgg attatttaat tatctagcat
     1141 acttcatgcg taatactttt tcctgttttg cagttaggcg cagtaaaata acaactttgt
     1201 caacgctcat attactctca agcagacgct ctgatgccat cattaaacca ttattaagtc
     1261 ccatttcaag gactggatta cttttatcgt tgatatcaaa aataaattta ttatcaggta
     1321 agtcaaacaa cactttaaca gcaccgaata attgctcaaa gttatcaata gcaacatcat
     1381 aaactggttt aattcccggt tttaaagaac gtcctctatg gttgaaccac atagaatgcc
     1441 aaccaccatt aaatgcaccc ataatatcat tatcatatga atcacccaca taaagtgtag
     1501 ttgaagggtt catatcaaat tgctctgctg ctaaattaaa aatctccttt tcaggctttt
     1561 gaaaaccagt tgcttgacta actataacac gttttggatc aacatagtca taaagtccca
     1621 attttttaac ttttttcaat tgatgttccg tagggccatt tgtaataatt cccattggta
     1681 cattttttga tttcaaaaag tcaagtgtca tacgcatctc atcaagcatt gtaatatttt
     1741 caagttcatg ttcgtatatt tcttgaaaat aaataccagt agcttcatca atttcacgat
     1801 aaccaaactc taaaagcgtt tctttgcatc gccagaaacg aaaatattct gttgtccatt
     1861 caccagccat tactcgtgga aagccaatat ctgaataatg acgaaagcga atataagctt
     1921 ggttaatagc gctcatatca aaatcaggaa agcatttctc cacagcaata cggtaaggtg
     1981 cttgttgatc gtaaattgta tcatcaacat caaaaacaat tgaagtaatc atgattatcc
     2041 ttttctttct gaattttcaa ttgttaatta tacctttttc tacccttatt ttcaaatggt
     2101 gaaaagtcta ttaaaaatga gaataacacc ataaactcat aaaataaatt atgaattatc
     2161 agtgtttttg tggtacaata ggatgaatac taacttggag gaaattatgt ctaaccaaca
     2221 tattgaagaa ttaaatgacc aacagattgt tcgtcgtgaa aaaatggctg ctttgactga
     2281 acaaggcatt gatccttttg gtaaacgatt tgaacgtaca gcaacatctg gtcaattaaa
     2341 tgaaaaatac gctgacaaat caaaagaaga cttgcacgac attgaagaaa ctgctacaat
     2401 cgctggtcgt ttaatgacta agcgcggtaa ggggaaagtt ggctttgcgc atatccaaga
     2461 tcgcgaaggt caaattcaaa tttatgtccg taaggattct gttggcgaag aaaactacga
     2521 aatctttaaa aaagctgatt taggtgactt ccttggtgtt gaagggcaag taatgcgtac
     2581 agatatggga gaactttcta tcaaggctac tcatattact cacctttcaa aagcactccg
     2641 tccacttcct gaaaaattcc acggcctaac tgatattgaa acaatctatc gtaaacgtca
     2701 tcttgattta atctcaaatc gtgacagctt tgaccgtttt gtaacacgct caaaaatcat
     2761 ttcagaaatt cgtcgcttta tggatagcaa tggtttctta gaggttgaaa cacctgttct
     2821 tcataatgaa gctggtggtg cttctgctcg cccatttatc acacatcaca atgcacaaga
     2881 cattgacatg gttcttcgta tcgcaactga attgcatcta aaacgtctta ttgtaggtgg
     2941 tatggagcgt gtttatgaaa tcggacgtat tttccgtaat gaagggatgg atgcaaccca
     3001 caatccagag ttcacatcta ttgaagccta ccaagcctat gctgactacc aggatattat
     3061 ggacttgaca gaaggtatta tccagcatgt tacaaaaact gtaaaaggtg atggaccaat
     3121 caattatcaa ggcacagaaa ttaaaattaa tgaaccattc aaacgtgttc atatggttga
     3181 tgcggttaaa gaaatcactg atattgactt ctggaaagaa atgactcttg aagaagcaca
     3241 agctttggca caagaaaaaa atgttcctct tgaaaaacat ttcacaacag ttggtcacat
     3301 cattaacgcc ttcttcgaag aattcgttga agacacttta attcaaccaa cttttgtctt
     3361 tggtcatcca gtcgaagtgt ctccacttgc taagaaaaat gatacagacc ctcgttttac
     3421 agaccgtttc gaactcttta tcatgactaa agaatatgct aatgctttta cagaattaaa
     3481 tgacccaatt gatcagctat ctcgctttga agcacaagca tctgctaaag aacttggcga
     3541 tgatgaagca actggagttg attacgatta tgttgaagca cttgaatatg gtatgccacc
     3601 tactggtgga ctaggtattg gtatcgaccg tctctgtatg ctgttaactg acacaacaac
     3661 tatccgagac gtcctacttt tccctacaat gaaataagaa tatattaaaa cccaccctta
     3721 tttaaggtgg gttttaatat tacctctact gaactttgtt aggtttctga tttatcgctc
     3781 acgcaatttt ttatcaaggt ttaccatttc aatagccgaa agagcacaat cataaccttt
     3841 atttcctgct tttgttcctg ccctctcaat agcttgttcg atattatccg ttgttagaac
     3901 accaaagata tgagggatat ctgactggct atttaaatga ccaatacctt ttgtcacctc
     3961 attacaaaca taatcataat gatcagtgct gcctttaatg actaccccaa gacagataat
     4021 agcatcataa tctttatata atgccatttt tctagccata taggggattt caaaagcacc
     4081 gggtacccat acaatatcta tatcttcttc tgaaacacca tgtctcaaca gtccatctac
     4141 tgctcctgat aataatttag atgtaatcaa ttcattgaaa cgtgagacta cgattcctat
     4201 tttcatttcg tttgccacta attgtccttc aataattgtc attttttctt cctcctttaa
     4261 tctaaaagat gtcccatttt ttctttttta gtttttaaat aattaaggtc ataagctgtt
     4321 gctgccacct gtaatggcac tctgtttttg acgcaaattc cttctgcttc aagttgttgg
     4381 attttatctg gattattggt tagtaagttt atagaattta tacctaaatc ttttaaaagt
     4441 tgagcactaa ctccatagtc tctctcatct ccttcaaaac ctaaagccaa attagcttct
     4501 aaagtatcta agccttcttc ttgcaaatga taagctttta acttattaat caaaccaatt
     4561 cctcgacctt cctgtctcat ataaagaagg acgccacgac cttcagcctc tatttgttgg
     4621 agtgcattag ccagttgttc cccacaatca catctcaagg aatgaaagac atcacctgtc
     4681 aaacattctg agtgtaaacg acataaaaca tcttcaccac tactaatatc ccctttaaca
     4741 agggcaacat gttccttatt tgccacaact tcactatatc cataagcctt aaaatggccg
     4801 aattgcgttg gtagctgaat ctcaatttct ctaaccaccc ctccctcttc ttgacgtcta
     4861 tagtcttgca attgttttat ggtagcgata gctaaatcgt gcttttgagc aaatgctaat
     4921 aactcatcct ttctcatcat actaccatct tcagccatta tttcacaaca aagtccacat
     4981 tcctttaatc cagctaaacg acatagatca acggttgctt cagtatgtcc atttctagct
     5041 aaaacacctc cttccttagc taacaaggga aataagtgcc caggacgtct aaagtcttta
     5101 gctttgctac tgtcgttagc aaccatctta gctgtcaaag ctctatcttc cgcagaaatc
     5161 ccagtactag ttgatagatg atcaatggat atggtaaaag ctgtttcatg attatcagta
     5221 ttatggctag ccatttgagc taattctaac ttttcagcat agtaattaga cagaggtaag
     5281 caaattaatc ctttcccaaa tttagccata aaattaatcg actcttttgt caccatttca
     5341 gccgcacaaa taaggtcccc ttcattctct ctattttcgt catccatcaa aactaccatt
     5401 tcccctgatt tgatgtcttg taaaagtttt tttattggac taaaggccat tcgctttctc
     5461 ctctctctta gtagccattt tctgataaaa aagaccaatc cattactgat gaagtgtgat
     5521 tagttctatt agtcggtgct aaaaatttct cagcatattt gcctaaaaca tccacttcga
     5581 tatttaataa atccccaacc ttttttaagg ataaattagt ttgagccatc gtgtggggaa
     5641 cgaccgaaac ttcaaaagaa tgctctgtaa atgataccac cgttaggcta atcccatcta
     5701 gagccaccga acctttctct ataatataag acataatact gggcggagca tcaattgaaa
     5761 atctgacagc aatatcatcc ttttggattt tttctatttt tccaacccca tccacatgtc
     5821 caagaacaaa atgcccccct aaccttgagt cacttctgag tgctaattct aagttaactt
     5881 tagaatgaag tcgtaaagca gctaagcttg ttcgcatcat actttctggc atgacgtctg
     5941 ctataaaagc tttttcactt ttgtcaagga ctgtcaaaca aacaccatta acagctacgc
     6001 tatccccttt tcttaataaa gggactagtt tgggaacatc aatacttaat tgttgggatt
     6061 ttatgccatt ccgaatacga ctgacttgac ccatttcctc aataattcct gtaaacataa
     6121 tgttctacct ttccttcaat aacaacgtca gaatccatac gactaagttc aatatctttt
     6181 actctgaaag cttggttaag ccaatccaag ccttctccag aaatagctgt ttttgctttc
     6241 agacctccta ttattttggg tgcaataaaa acaattagtc tgttaacaat accagcttct
     6301 aaagctgaaa aatgtaaact acttcctcct tcaataagaa ggctatcaat cccttcttgt
     6361 cctaaaatag tcattaatac tttcaaatcc agctttccat cttttcttgg gaccttaatc
     6421 aaacggcaac ccatttcttt taatgtttgt tgctgagcaa gattatctga acatgttgca
     6481 atccatgttg gtattgtttt agcgctctta actaggtaag agtctaaagg tatcttcaac
     6541 tgactatcac atacgatcct cacaagcgct tcacctttag gaatacggca agttaaacgt
     6601 ggattatcag ctaatacagt attgataccg accataatag cactacattt ttgcctcaat
     6661 ttctgaacag attgtcttga gtgctcattg gatatccact tagaatcgcc cgtctttgta
     6721 gctatcttcc catctaaagt catagcatat ttcaaataaa caaaaggctg cttataggtc
     6781 atgtgaaaaa taaatctttc attcagagca tcgcattctt ctcttaggat accaacttca
     6841 acatttaaac cttcttttct taacaaggca atcccttttc ctgaaactaa aggatttgga
     6901 tctaaacttc caactactac ttttttgata ccagatttaa taagagcttc agtacaaggt
     6961 ggttgcttac ccacatgaca gcatggttct aaagtcacgt acagagtact tccagagata
     7021 tcctcatcag catttttaat agcctgtctt tccgcatgta aatctccaaa tcgtttatga
     7081 taacctttgg aaattatacg atcatcttta acaataacag cacctactaa tgggttggga
     7141 gcaacgaacc ccataccttt ctcagcctct ttcaaggcta atgccatgta gtcttctttt
     7201 ttcatcattt cctttttcct tctaaaataa aaacccttag cttctaaaat agaaaactaa
     7261 ggggagggat gagaagtcgt tgattatact aattagcatt atattttagg tagcacaaaa
     7321 aaactaaaga ctccctatag aaaactatag ggagtctctc attaatccag cacaaataaa
     7381 ctgcaagtag cagacaatca ctgaattctt ctctcatcca gactttactg tcggtactgg
     7441 aatcacacca gttcatgctc gaaagctcgc ggactatacc gccggttagg attttcacct
     7501 gaccccgaag aaattctatt tgacagttat tttactaaac tcatttttat ttgtcaataa
     7561 aaataatgtg ttttttatta taaaataata attaagtagc tcatctattc ttatctttaa
     7621 aa
//
LOCUS       DATRSG010000075         7331 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRSG010000075 DATRSG010000000
VERSION     DATRSG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.075"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            4..237
                     /locus_tag="VBD89_001772"
     CDS             4..237
                     /locus_tag="VBD89_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001888659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7136279.1"
                     /translation="MIFYDYRFGMISNDAVNKFLASSLEKLEIESKMSSTGARHTYGS
                     YLLANGVDIWAVAKLMGHKDIKQLIETYGHLLL"
     gene            complement(373..443)
                     /locus_tag="VBD89_001773"
     tRNA            complement(373..443)
                     /locus_tag="VBD89_001773"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Thr,seq:ggt)
     gene            complement(514..1348)
                     /locus_tag="VBD89_001774"
                     /pseudo
     CDS             complement(514..1348)
                     /locus_tag="VBD89_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            1659..1913
                     /locus_tag="VBD89_001775"
     CDS             1659..1913
                     /locus_tag="VBD89_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO7136280.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            2022..2333
                     /locus_tag="VBD89_001776"
     CDS             2022..2333
                     /locus_tag="VBD89_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136281.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            2601..3170
                     /locus_tag="VBD89_001777"
     CDS             2601..3170
                     /locus_tag="VBD89_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7136282.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWE"
     gene            3268..3852
                     /locus_tag="VBD89_001778"
     CDS             3268..3852
                     /locus_tag="VBD89_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7136283.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVT
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASVLVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKKRR"
     gene            3849..4415
                     /locus_tag="VBD89_001779"
     CDS             3849..4415
                     /locus_tag="VBD89_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7136284.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFLNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVAFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENHLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     YKINNTCLTATETAYLIIQKINQIKEKN"
     gene            4415..7066
                     /locus_tag="VBD89_001780"
     CDS             4415..7066
                     /locus_tag="VBD89_001780"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO7136285.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYADSSIVTAAYPTVNPAFENAAAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLGNERFLANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
BASE COUNT         2342 a         1280 c         1513 g         2196 t
ORIGIN      
        1 caattgattt tttatgacta taggtttggt atgatatcaa atgatgctgt taacaaattc
       61 ttggcaagct cattggaaaa attagaaata gaatctaaga tgtcatcaac aggtgctaga
      121 catacttatg gaagttattt gttggctaac ggagtagata tttgggcggt tgcaaaatta
      181 atggggcata aagatatcaa acaattaata gaaacatatg gtcatttgct tctgtaagtg
      241 gaagaaaagg aaaatgatca gcttttaaaa attcttaata ataaaggtag tttttaaaat
      301 gactatcaag aacaaattaa gaacaaaaat aaaagaaacc ctattataat aaggtttcac
      361 tcatatattt tctgccacca ccgagaattg aactcggaat tgagcattac cattgctcta
      421 ttataccatt taactatagc ggctactttt ctatgatact ataagatttt gacgaaatca
      481 agtatttctt tacttagaaa aatagtcgtt tttttagtct aattctattt ggagaaattt
      541 actattttca atagctctta aatggtgtgt ttcttgtgga gagaggctaa taaggctatc
      601 tttttcaagg atatgtttct gatcaccaag gataatttca agttttcttt ccaaaacatt
      661 tattaatttt ggatgagggc atcgttcatt tttcagtccc cggctgatga ttttaatggt
      721 attgaataaa ccttcatctt taatcattcc taacggggac ataagactta tttttccaaa
      781 ttgatattct gaactttaaa aaccattttg taagaataaa ttttgccaat catctatttt
      841 tagaggagca acgttaacat tcatagtatg tctaagttga tcgatcagct tggcagtgtc
      901 ttcatcttga taagaaacat catgtgtgag taaacgtcca ccaggtttta ggacacgtaa
      961 gtactctttt atggctttct ccttagctga attgttaagc atcgttaaca ttgcttcgtt
     1021 gataacgata aactattatc aggaaagggc agtctgagtg catttccctg aataacttca
     1081 ataaattctt ctaattgggc ctttttgaca ttttctttgg ccttttctat tactttacta
     1141 tctagatcaa gcgctgtaat gtgacaggaa aatgctttag cgatatcgat ggaactggta
     1201 cattgattac aagctacttc tagaacacga ctgtcagcac taaagtgtcc ttttttgata
     1261 agccaattcg ttgcttggac acctcctggt cttaagcgtt ttttcctagt cgtgctaaga
     1321 attgatgtcc aatttcttta tgtggcaata agatcacctc tttcttttaa tagtaatatc
     1381 attttttctt aattataaca catagatgct aagataacaa ggagtaatag tgattgaatg
     1441 gtataagtga tgaattttag agagcgtttt cttttaacta ggggtgtgat gccaaaacta
     1501 gaatggcata gagaaccata catctcaaaa ggtcttcctt agataaaact agttgttttt
     1561 atatctgtca gaaaaatcat aatttatttt tgatataatg attttttgag tatttaattt
     1621 atatgttata atgaaaaaaa ctaaaaaaga ggaatatcat gtcatacgaa caagaatttt
     1681 taaaagactt tgaagagtgg ttacaatcgc aaataagtat caaccaaatg gcaatggaca
     1741 gtgcaaagaa agttttagag gaagataagg atgagcgagc agcagatgcg tatattcgtt
     1801 atgagagtaa gttagacgct tatcgctttt tgcaagggaa atttaataac tatcataacc
     1861 aaaaatcttt ccatgatttg cctgatggtc tttttggaca acgtcattat taaaaggttt
     1921 atcgctagaa agcttgtcaa ataggcattt ctatgataca ttgttatagt agtttttaga
     1981 tactatgata gatttcataa atgaggagaa agcattatac aatggcaaag cgtattaaac
     2041 cgaataaaaa aagagagctt ttgaaagcta aagggatcaa ttggaaggat gttcgactat
     2101 ctcaaacttt acaagaagtc gcatcaccga aaaaaaaggc tcctgaagcg ccaaaggcag
     2161 tcgatctttt agaaggttta actgctaata aacaagatgt tttaaaagaa gctggccttg
     2221 tcagccttga agcgtttgcg aaagtaagtg aagcagatgt tttagcacta aaaggtattg
     2281 gtccagcagc tattaaacag ttagttgata atggtgctgt ttttgcaaaa tgatttgatt
     2341 caccttgcga aagtagtctt attttgctag aataaattaa cttagaggtg ttttgaaatc
     2401 ccatttgtca caggaagcgg tcatactgag aaatccgcac aaatgtcaaa gcttgttgct
     2461 aagtccaata attgaaacaa aacgagttgc ggatgttatc atccgaattt gggtggtacc
     2521 gcggaaatcg taatacgaaa ttcgtccctg tcagaattga cagggatttt ttgtatttag
     2581 gagtgaggag attgaagtat atgcagattc tagaagattt cgatggcaaa gcattaccaa
     2641 aactagaaac ggatcgactt atcttgcgtc agcgaacggt gggggatgtc ccagccatgt
     2701 ttgattacgt ttgcttggaa gaggttgcct atccagcagg gctcagtcca atagcttctt
     2761 tagaagatga gtatgattat tttgaaaatc gttactatca aaatctggaa aaggcaaaat
     2821 taccatctgg ttatggtatt accgttaaag gaagtgaccg gattatcggc tcttgcgact
     2881 ttaaccaccg ccatgaagac gatgttttcg agattggcta catgcttcat cctgactatt
     2941 ggggtcatgg ttatatgaca gaagctgtcg cagccttgat tgaagtcggt tttaccctcc
     3001 tcaatctcca caaaatcgaa atccgctgct atgattacaa taagcaaagc cggcgcgtgg
     3061 cagaaaaact aggcttcacc cttgaagcca ccatccgtga tcgtaaggat aatcaaggta
     3121 atcgctgtgt caatttgata tatggtttgc tgaggagtga gtgggagtaa ttattctact
     3181 cttcatagaa gttactagat agactaattt ggctgtgcag ggtacttagc taactcatca
     3241 ataatatcgt agatatggag gagacaaatg attaagatac ttggagaaat cgtagataac
     3301 cagctacctg ttgtggaaac gaatcgtctg ttgttgcgtc aacgcaagtt agaagatgct
     3361 aaggagatat ttgaatttgt caaacttgat gaggtgactt atccagcagg ttttccggct
     3421 gttaaaagct tggaagaaga gataacctat attcaagaga tttatccaac taatcttgaa
     3481 aaagaaaagt taccctctgg ttatgctatt actctaaaag gcgatgataa ggtaattggt
     3541 tcagtagatt ttaaccatcg ccacgaagac gacatttttg aaattggcta cctgctccac
     3601 cctgattatt ggggacaagg cattgtgcca gaagcagctt cagtccttgt ggagataggt
     3661 ttcacactgc taggactaca taagattgag ctgggatgct atgactataa taagcaaagt
     3721 caggcggttg cgcgcaaatt aggttttact ttagaggcta atattcgtga tcgaagagat
     3781 gcccaggggc aacgttgtgg cgatatgcgc tttggtttac tgcgaagcga gtgggagaaa
     3841 aaaagaagat gaatattatt atcatcggag cacaagcttc tgggaaaatg acaattggtc
     3901 aggaaatcgc aaaacagaca ggcatgacct tattccttaa ccatgattct atagattttg
     3961 ttttacgctt catgccatgg tctccagata gtattgctct aacggagtct attcgcttta
     4021 aattttttga aacgtttgct aagactggtc aagaaatgat ttttactatt gtaattgatt
     4081 tcaatgattc aagagatgta gcatttctag aaaagattca aacagttttt caatctcaca
     4141 accaagaggt attatttgta gaattggaaa cagagctaag tgagcgtctc aaacgaaatc
     4201 gcacagaaaa tcacctcaag cataagcctt caaaacgtga tatcaaatgg tcagagaacg
     4261 atatttgttc aaccatggat tacgctatct ttaatcctga agtagcccca gaagcgctta
     4321 catattatta taaaatcaat aatacttgct taactgctac tgaaacagca tatcttatta
     4381 ttcaaaaaat aaatcaaatc aaggagaaaa actaatgtca aaagaactat caccaaaata
     4441 caatcctgcc gaggttgagg caggccgtta tcaaacttgg cttgaccaag atgttttcaa
     4501 gccatcaggg gatgctgagg ctaagcctta ttctattgtg attccaccac caaacgttac
     4561 gggtaaattg cacttgggtc acgcttggga tacaactcta caagatatca ttattcgtca
     4621 aaagcgtatg cagggctttg atacactctg gcttccaggt atggaccacg ctggtatagc
     4681 cactcaagct aaagttgagg agcgccttcg tgagcaaggc atttcacgtt atgatcttgg
     4741 tcgtgaaaaa ttcctagata aagtttggga atggaaagac gagtatgcag caactattaa
     4801 atctcagtgg gggaaaatgg gtctttcggt tgattatagc cgtgagcgtt ttacccttga
     4861 tgagggactt tcaaaagcgg taagaaaagt ctttgttgat ttgtacaata aaggttggat
     4921 ctatcgtggg gagttcatca ttaactggga tccagcagct cgtacagccc tttcagatat
     4981 tgaagttatt cacaaggatg ttgagggtgc tttctatcat atgaattata tgctggaaga
     5041 tggctcacgc gccctagaag tagcaacaac tcgtcctgag actatgtttg gagatgtggc
     5101 tgtagcggta aatccagaag atccacgtta caaggatttg attggtcaaa atgttatcct
     5161 tcctatcatc aataaaccta ttccaatcgt cgctgatgaa cacgctgatc cagaatttgg
     5221 aacaggtgtg gttaagatta cgccggctca cgaccctaat gacttcgctg taggtcaacg
     5281 ccacaactta ccacaagtta acgttatgaa cgatgatggg actatgaatg aacttgcgga
     5341 tgaatttaat ggtatggatc gtttcgaagc tcgtaaggca gtagtcgcta aattagaatc
     5401 gttaggaaac ttagtaaaaa ttgaaaaaat gactcattct gttggtcatt cagaacgaac
     5461 aggcgttgtg gttgaaccac gtttatctac tcaatggttc gtcaagatgg atcaattggc
     5521 taagaatgcc attgccaacc aagatacaga agacaaggta gagttttatc caccacgatt
     5581 taacgatact tttatgagct ggatggaaaa tgtccatgac tgggttatct ctcgtcaatt
     5641 atggtgggga caccaaattc ctgcatggta caacgtcaat ggtgagatgt atgttggaga
     5701 agatgcccca gagggtgacg gatggacaca ggatgaagac gttctagaca catggtttag
     5761 ttctgcttta tggccgtttt caacaatggg atggccagac accgaagcag ctgacttcaa
     5821 acgctacttc ccaacgtcaa ccttggtaac tggatacgat atcatcttct tctgggtatc
     5881 gcgcatgatt ttccaatctc ttgaatttac aggacgccaa ccattctcaa atgttcttat
     5941 tcatggactc atccgtgatg aagaaggacg taaaatgtct aaatcacttg gcaatgggat
     6001 tgatccgatg gatgtcattg agaaatatgg tgcggatgct cttcgttggt tcctctcaaa
     6061 tggttcagcc cctggtcaag atgtgcgttt ttcatacgaa aaaatggatg cttcgtggaa
     6121 tttcattaat aagatttgga acatttcacg ctatatcctt atgaataacg aaggtttaac
     6181 gttggatcaa gccagagaaa atgtggaaaa agttgttaat agtcaagttg gaaatgtaac
     6241 agaccgttgg attctccaca atttaaatga gactgtgggt aaagttactg aaaactttga
     6301 caagtttgaa tttggtgttg ctggtcacat cctttacaac tttatctggg aagagtttgc
     6361 taattggtac gttgagttaa caaaagaggt tctttatagt gacaatgaag atgagaaagt
     6421 tgttacacgt tctgttcttc tttatacctt agatcaaatc ttgcgtcttc ttcatccaat
     6481 catgccattc gtgacagaag aaatctttgg tcagtatgca gatagctcaa tcgtaacagc
     6541 tgcttaccct acggttaacc cagcctttga aaatgctgct gcccacaagg gagttgaaag
     6601 cttgaaggac ttgattcgtt ccgtgcgtaa tagccgagca gaggtcaatg ttgctccatc
     6661 taaaccaatc actatcttgg ttaagacaag tgacagcgaa ctcgaaagct tcttcaaaga
     6721 caatagtaac tacatcaaac gtttcacaaa cccagaaaca cttgaaatca gctcagctat
     6781 tgccacacca gaacttgcca tgtcatcagt catcacagga gctgaaatct tcctcccact
     6841 tgctgacctc ctcaacgtag aagaagagtt agcacgactt gaaaaagaac tagccaaatg
     6901 gcagaaagaa ctggacatgg taggcaaaaa actcggcaac gaacgcttcc tcgccaacgc
     6961 caaaccagaa gttgtccaaa aagaaaaaga caaacaagcc gactaccaag ctaaatacga
     7021 cgcaacccaa gaacggattg tagagatgca aaaacttgtt aaatagttaa aatcatccat
     7081 tacttccgca gagtcctgcc gctagaccat cagtctaagc gacagggctt tttgctatat
     7141 tcaaagattg tgaaaaatta atatatatga tgcctacttc ttgcgtctga aatacaagaa
     7201 gtgaaaatga tttggccata aagaaaaaca ggcagaaacc caaatgatta atgcctattt
     7261 cttatatttt ttcgtcaatt attttataaa taaaattgag gtacaaacca gtttttaaaa
     7321 tgataaggtg a
//
LOCUS       DATRSG010000076         7247 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRSG010000076 DATRSG010000000
VERSION     DATRSG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.076"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..1175
                     /locus_tag="VBD89_001781"
     CDS             <1..1175
                     /locus_tag="VBD89_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7136286.1"
                     /translation="KSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHYLILSNFS
                     KTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQINASEQDY
                     FIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFNLDKIKQF
                     SKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELRSEKNYSI
                     MIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFYTQQVHSL
                     KKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEKHITIQQY
                     FLNLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQKPKTI"
     gene            complement(1370..1894)
                     /locus_tag="VBD89_001782"
     CDS             complement(1370..1894)
                     /locus_tag="VBD89_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7136287.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            2026..2889
                     /locus_tag="VBD89_001783"
     CDS             2026..2889
                     /locus_tag="VBD89_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7136288.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            2974..3828
                     /locus_tag="VBD89_001784"
     CDS             2974..3828
                     /locus_tag="VBD89_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7136289.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAINQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            3938..4965
                     /locus_tag="VBD89_001785"
                     /pseudo
     CDS             3938..4965
                     /locus_tag="VBD89_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            4981..5370
                     /locus_tag="VBD89_001786"
     CDS             4981..5370
                     /locus_tag="VBD89_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7136290.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            5380..5775
                     /locus_tag="VBD89_001787"
     CDS             5380..5775
                     /locus_tag="VBD89_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7136291.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEENKKAVSLEPGNVIVTDKGVKHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSLEEYSRLG"
     gene            5803..6120
                     /locus_tag="VBD89_001788"
                     /pseudo
     CDS             5803..6120
                     /locus_tag="VBD89_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            6207..6491
                     /locus_tag="VBD89_001789"
     CDS             6207..6491
                     /locus_tag="VBD89_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO7136292.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
     gene            6730..7221
                     /locus_tag="VBD89_001790"
     CDS             6730..7221
                     /locus_tag="VBD89_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7136293.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKT
                     NLI"
BASE COUNT         2530 a         1188 c         1225 g         2304 t
ORIGIN      
        1 tgaaatcatt acacagtctt ctaggtctaa ctattactgt ttgtgatcaa aatttttctg
       61 ttatcagaga gtataaatcc gagaaaacta tttcactttt ttacaatcat taccttattt
      121 taagtaactt tagtaaaact caacatgatt ttttatttca ttatggctct ttaggagaac
      181 tttttcttgt ccaccatatt caacaatatt atattatcat cggtccctgg cgtagcaatg
      241 ttattgaccc tttacttctt aagaaaaagc tgactgaaac acaaatcaat gctagtgaac
      301 aagactactt tattgataga ctatcccaat tacctttctt ctcacttagt caaatccgag
      361 aattactaat agtaaccaat tattgtctaa ctggggttgt gaaagataaa ttatcagagc
      421 ccttacatta ctacacgaaa gggtggagca actcctttaa tctagataaa attaagcaat
      481 tttctaaaca gaatatgagt tcttacaaat atcagtatca ttttgaaaat aatattctaa
      541 aagctgttaa atcaggtagt gaatttcttt tgaaagaaac agtggaacaa tttagcaatt
      601 ctatcgtacc tataatcagt ggagatgaat tgcgatctga aaaaaactac tccatcatga
      661 tttatgatcg tctctcacag gctaccatcc aagcaggact tgatatcgaa acagcttatc
      721 gagcacgaga tcgttttata aaagaaaacg agtcaactat aagtctaaat gaagttttaa
      781 aattacgtga tactgctatc ttattctata ctcaacaagt tcattcttta aaaaaacatc
      841 tcgaaacccc tcattctcaa actattgtcg cagtgattcg atatcttgaa aataatctaa
      901 atcgctttat taaaacagaa gaaatagcta aagaatgtca catgagtgaa tcgaagttac
      961 gaaaactatt taagcaagaa aaacacatca ctattcaaca atattttcta aacttaaaaa
     1021 tcgaagctgc taagcaatta ctagatgaaa ataagaaagt agaagaagtt tccaatttac
     1081 ttggattttc cacttcttct aatttttcaa ggacatttaa aaaaatagtg ggaattagcc
     1141 cactagaata taagcaaaag cctaagacaa tatagtaatc gaaaaatatt cattttgctt
     1201 atggtgacat caacaatccc aaagaaactc tttcatcaag gggacttttt tatctcccga
     1261 attatactca tttaatctaa taatttcaac tcgctatatt agacaatcaa tccattcatc
     1321 acctctgata acgctaatga aataagagaa tctcccaact ctctataatt tacctctcta
     1381 ccatagttaa ataatatttt agtaaatctt caacagtcat tttctgtctt tgatgaagcc
     1441 accactttaa ggtctcgata aaactggata aaagaaattg ttttaagaaa tcttcaggaa
     1501 tgtccacctt tgtaatatac tcctcacgca atctagggta aacatcgtgt tctaattcac
     1561 ttctaaagcg taataaaaaa tagggatcgt cagataataa taacgtagcg atactatctt
     1621 gattttgttc aaaatgtttt aagatatgaa ctaaatactc ttcaaacgta acatctctac
     1681 cctgcttaaa taagtggtga aaaaggtctt cacagagctc cttgagtaac acttctttgc
     1741 tttcatagtg agagtaaaaa gttgatcttc ctacatttgc aagtccgatt acatcttgaa
     1801 ccgttatttt agaataatca ttttcctgta gcaagcttat aaaagcatta taaatcgcta
     1861 cttttgtttt ctgaatttgt ctatctttta ccatatagga cactttctaa gttttgttca
     1921 gtattgaaca agtgttcttt tttgtttcta gcaattctct tttatctcct agataataga
     1981 agaaagaatc acactacaat accagtataa caaaaggaga aaaatatgac atcaaacaaa
     2041 aaagtggcta tcgcttttat attaaatatc agcttttccg tccttgaatt catctttgga
     2101 agtctattct tctcaggagc tatcttagcc gatgctgttc atgattttgg tgatgcaata
     2161 gctattggca tctcagctac tttagaaaaa aaatctaaga aagatgaaga tacgatattt
     2221 tcattaggtt ataaacgttt tagcctttta ggagctctta tcacaagtct aattcttata
     2281 agtggatcta tcttagtcat gatagaaaat attccaaaat tgtggcatcc tactcctgtt
     2341 aattatcacg gcatgtttat tctagctgtc atcgctatta tcattaatgg tctagctagt
     2401 tttattctcc attctggtca aagtaaacat gaggaaatat taagccttca ttttttggaa
     2461 gatattctag ggtggttagc tattattgtt atatctctta tcttaaattg gaaaccgttg
     2521 tatatccttg atccattatt atcagttgcc atatctactt tcatcttatc taaagcactt
     2581 cctaaattac tatcaaccct gaaacttttt ttagatggtg ttccagattc catagattac
     2641 gctgcattac atgatgaact taagggactt tcgcaagtaa gatctatcaa tcaactcaat
     2701 atctggtcaa tggatggtat tgataatcga gcaatcattc actgttgctt aaatcaattg
     2761 atatctgaaa aagattgtaa aagagctatt cgaacgattt gccaacatta taagataaac
     2821 gatgttactg ttgaaatcga ttattcttta agagaacatc aaaatcattg caaaccatta
     2881 aaaaactaac ctgagtgtta gttttttcct tgacttggag ttaactctaa gtgttaagat
     2941 taggtcaata ctaatgaaag aaggaaaata aagatgacag atacaatgat attaaatgac
     3001 aatcataaaa taccaacttt aggtttaggt acgtggatga tagataatga tagggtagct
     3061 caagttgtta aagatgctat cgaattaggc taccgccata ttgatactgc acaagcatac
     3121 aacaatgaaa aaggggtcgg tgtagggatt aaagaatctg gtattacacg tgaatcttta
     3181 tttgttacga ctaaaattgc cgctgaacat aaagattatg aaagtgctac aaaatcaatt
     3241 gatcgatcac tagaagcttt agggcttgat tatattgatt taatgattat tcatagtcca
     3301 cagccttgga aggaatggcg tgaaacagat aaacattttg accaaggaaa tttagaagct
     3361 tggagagctt tagaggatgc tcaaaaatct ggtaaggtaa aatccatcgg tgtctcaaac
     3421 tttttagaaa aagacttaga aaatatctta aaaaatggtt atgttaaacc tgctatcaat
     3481 caaatcttag ctcacatcgg aaatacacct ttcgacttga ttgattactg tcaaagtaaa
     3541 ggtattcaag ttgaagctta ttcaccaatt gcacatggac aagcattgaa atctgatggt
     3601 attcaaaaaa tggctgaaaa atatggggtt agtgttgctc aattatgtat tcaatattta
     3661 ctacaattaa acctgatcgt tttaccaaag gcttctagta aagaacattt acagtctaat
     3721 ctagactttg actttgtaat tagtgacgaa gatatgtcga tattaaaatc acttatgttt
     3781 gacgattatg gggaattttc taacttccct gtttttagtg gaaaatgaaa ccatatgcgg
     3841 atttttacag atgtttaatt ttcaaaattt ttaagttttt cttgacttga agttaactct
     3901 aagagttata atgaagttat agaaaagagg taaggttatg aaagtagcta catttattga
     3961 acctggcaaa atggttataa ctgatacacc aaaaccagtc attgaacaag agacagatgc
     4021 tgttatcaaa attgttagag cctgtgtttg tggttcagac ttgtggtggt atcgtggtat
     4081 ttcaaaacgt gaaagtggtt cttttgcagg tcatgaggct attggtatcg ttgaggaagt
     4141 tggtactaaa gtaactgacg tgtcaaaagg tgattttgtt attgttccct ttacacatgg
     4201 ctgtggccaa tgtccgtctt gtaaggctgg atttgatgga aattgtacaa atcatcaagc
     4261 tgaaaaaaat gtagatacca aggtcaatat ctacgttata ctaatgcaaa ctgggcattg
     4321 gttaaaattc ctggtcaacc ttctgactat gataatgaga cacttaactc tcttctcacc
     4381 ttatcagatg taatggcaac tggttatcat gctgcagaag ttaaagaagg tgatacggta
     4441 gttgtcatgg gagatggtgc tgttggactc tgtggtgtta ttgctgctaa aatgttaggt
     4501 gctaaccgta tcattgcaat gagtcgtcac aaagataggc aggaactagc attgactttt
     4561 ggtgcaacgg atattgtcga agaacggggt gacgaagccg ttaaacgtgt tttagattta
     4621 accaatcaag caggtgccga tgctatttta gaatgcgttg gtacagagca gtctgttgat
     4681 acagctaccc aaattgctag gcctggtgct gtcataggac gtgttggtat cccacaaaat
     4741 ccagacatga ataccaataa tttattctgg aaaaatatcg gccttagagg tggcattgct
     4801 tctgtaacaa catttgataa gtctgtcctt ttagatgctg ttctaactca taaaattaac
     4861 ccaggcttag tttttacaaa atcctttgta ttagatgata ttcaaaaggc ttatgaagca
     4921 atggataaac gtgacgctat caaatcctta gtgattgttg actgaatatc gatgttaaaa
     4981 atgcatatca aagaagttag taaaaagact ggcattacgt cagataccat taggtattat
     5041 gagagaatta atcttattcc ccctattcat cggagtgaat caggaattcg tgagttcaca
     5101 caaaacgata ttgatatttt agaatttgtc cgctatttta gaggcgcagg tgtttccgtt
     5161 gaaagtctca ttgattatat tcatttggtt gaaaaaggcg attccacaat cccagctagg
     5221 ctagaaattt taaaagataa acgcaacaac cttgaagata ggcttcaagg cctccaagaa
     5281 gcacttaatc gtctaaatca caaaatagac aattatcaaa aagttcttgt tcctagagaa
     5341 caagaacttt ttgatccaac aacgaaatga ggaaatctta tgcaagaaac aactaagtct
     5401 atctttccta agggtgaaaa aaatccatat ggtgaattct ttattaggca atcctatctc
     5461 gcagcattag caaaatctcc agatgggaat gtttcagttg gaaatgtaac ctttgaagct
     5521 ggctgtcgta ataattggca tgttcatttg gacggttatc aaatcctcct cgttacagaa
     5581 ggttcaggtt ggtaccaaga agagaataaa aaggcagttt cattagaacc aggtaacgtg
     5641 attgtaactg ataaaggcgt taaacattgg catggtgcta aaaaagattc tgaatttgca
     5701 catattgcga ttacagctgg taagagtgag ttttatgaag ctgttagtct tgaagaatat
     5761 tcaagactag gttaaattca agtaaggagg aatcaatcca ttatgaaaga aaaacaaaca
     5821 gccggacgtc gtcaattaga agaatttgca cctgaatttg ccagatataa tgatgatatt
     5881 ttatttggtg aggtttgggc aaaagaggat catttaacag ataaaacacg ttctattatt
     5941 actatatctg ccttaattag ctgaggaaat cttgaacagt tagagcatca tcttcaattt
     6001 gccaaacaaa acggagttac taaagaagaa attgctgaca ttatcactca cctcgctttt
     6061 tatgttggat ggccaaaagc ttggtcagcc tttaataaag ctaaggaaat ttggatataa
     6121 aataacagac atagcaaggg agatactttc ccaaaagcca aaaagaaaga tcaaccaatt
     6181 attaaaccaa ctattaggag aatgatatgc ttactattca agaaatgaaa gatacaacct
     6241 ttgaattaca agaaaatttt agaaggttaa attatcctat taaacaagtt gctaaagact
     6301 tgcaacttaa tatcagtgaa gttgaatctc ttttaagtct tgatgttact tatcctggag
     6361 atgtttgtat gctgagagat tatctagaag atatgttgaa aaaagagggt aaagaagttt
     6421 atccattttc tagattagca tctcattcag caaatcgttg gtacccttat gagacaccat
     6481 ggcgttatta aagactaaag attaatccat catctttaga gaaataaaca aaaatagtca
     6541 atagattctt tttagaaaaa agctataaaa taagtgcctc ttagagaata ctctatatca
     6601 taagatttct tctttgaacc atatcattcc aaaagaaaat tatgattgct aacaagacaa
     6661 ttattctaaa aagaaactat tttaacgact ctataattac ttaaggaatc ataaagaata
     6721 aagattttta tgctatcatt tcaaattgtt gaaaagccag caatgatact tgctggagta
     6781 actttagaaa atgtaaagtc aaatcaagaa tgtcctaaac tttgggaaaa attacataaa
     6841 caatataatc catcaaaact aaaagaaaaa ggtatccagc aagccattgg catttgtaaa
     6901 acacaacctg attttcgttt cgattacagt gccacttatc aagtagagac aagtgtccaa
     6961 gccccaaaag ggttagaaat tattaggatc ccaagcgcta cctatgcagt catttctgtt
     7021 aaaggtccaa tgccatcgag tcttcaagaa acatggcgaa aaataataca aggatttttt
     7081 caagagaata atctaaaacc agctaactcg ccaaacttag aaatttattc atctcaacat
     7141 ccacaagata ctgattatca aatggaaatt tggcttgcta tcgcagatat tactaataac
     7201 tcgaaaacaa acttaatcta gtattgctta atataaaaaa gcccctt
//
LOCUS       DATRSG010000077         6756 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRSG010000077 DATRSG010000000
VERSION     DATRSG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.077"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..546)
                     /locus_tag="VBD89_001791"
     CDS             complement(<1..546)
                     /locus_tag="VBD89_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7136294.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMVDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDIL"
     gene            652..1572
                     /locus_tag="VBD89_001792"
     CDS             652..1572
                     /locus_tag="VBD89_001792"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO7136295.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTEKELESIKSLNDNINI
                     QDVIDIYLPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAVGKSTTSR
                     LLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLLNFLDTIK
                     NGITAKIPIYSHEIYDIVPNQLQTIETPDFLILEGINVFQNQQNHRLYMNDYFDFSIY
                     IDAENKQIEEWYLQRFNSLLQLAEADPSNYYHKFTQIPPHKAMELAKDIWKTINLVNL
                     EKYIEPTRNRADFIIHKGKHHKIDEIYLKK"
     gene            1626..1874
                     /locus_tag="VBD89_001793"
     CDS             1626..1874
                     /locus_tag="VBD89_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO7136296.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
     gene            complement(1958..2791)
                     /locus_tag="VBD89_001794"
     CDS             complement(1958..2791)
                     /locus_tag="VBD89_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7136297.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVTKTSSHINSIKDMKGKKLGAQSGSSG
                     FDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLKNNRIDGLLIDEVYANYYLKQEGNI
                     KAYYFVKTAYQGENFVVGARKVDRRLIEKINKAFKQLHNKGRFQKISYKWFGEDVYSK
                     E"
     gene            complement(2805..3434)
                     /locus_tag="VBD89_001795"
     CDS             complement(2805..3434)
                     /locus_tag="VBD89_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7136298.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            complement(3444..4085)
                     /locus_tag="VBD89_001796"
     CDS             complement(3444..4085)
                     /locus_tag="VBD89_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7136299.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            complement(4220..4549)
                     /locus_tag="VBD89_001797"
     CDS             complement(4220..4549)
                     /locus_tag="VBD89_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO7136300.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFIKK"
     gene            complement(4712..6526)
                     /gene="glmS"
                     /locus_tag="VBD89_001798"
     CDS             complement(4712..6526)
                     /gene="glmS"
                     /locus_tag="VBD89_001798"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO7136301.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
BASE COUNT         2270 a         1284 c          961 g         2241 t
ORIGIN      
        1 taaaatatcg caattaccaa aagtatctag cattttagct tttgcacttg gagcgccttg
       61 tttcttttga atgacaatgg taaggctacc gcctgtatta aggtaacaga tactttcttc
      121 gataatagaa tgaacaactt gctttccagc acgaataggt gggttgctta taatatagtc
      181 gaaagtttta gaaatatttt cataaatgtt tgactgaaag acttcaacta ctactccatt
      241 gcgtgttgca tttttcttag ctaattccaa tgctctcgta ttaatgtcaa ccatagttgc
      301 tttaactcct tgcactttgg ctagagaaat tcccagcgga ccgtatccac acccaaggtc
      361 taacaaggat ttattttttt caaaatgtaa actatttaga aggacttgac taccataatc
      421 tatcattctt ttagagaaaa ctcctgcatc agttaaaaaa ctgaatgatt ctcctaataa
      481 tttgacattt agctcatgaa tatcatgttc aacattagga ttctctgtat aatacatatt
      541 agccataaat aattctaacc ctccaatttc gccatattat tttaccatat tttgaaacgg
      601 ttttcaatca ctcaataaaa tgttataata gctttcatat aggagtttga catgaataat
      661 gaatttatta atttcgaccg tatttccaga gaaaattgga aggacttaca tcagcaatcc
      721 caagctttac taactgaaaa agagcttgaa tctatcaaaa gcttaaacga taatattaat
      781 attcaagatg ttattgacat ctaccttcct ttaattaacc ttattcaaat ctataaacgt
      841 agtcaagaaa atttatcttt ttctaaagca atatttctta aaaaggaaaa ttatcaacgt
      901 ccttttatta tcggcatatc agggtcagtt gctgttggaa aatcaactac cagtcgctta
      961 ctacaattac taatttcacg aacttttaaa gatagtcacg tcgaattagt cactactgat
     1021 ggctttttat atcctaacga gaaactcata caaaatggaa ttttaaaccg taaaggtttc
     1081 ccggaatcct atgatatgga gtcacttcta aatttcttag atactataaa gaacggtata
     1141 accgcgaaaa tcccaattta ttctcatgaa atctacgata tcgttccaaa tcaattgcaa
     1201 actattgaaa caccagattt tttaatttta gaaggaataa atgtctttca aaaccaacaa
     1261 aaccatcgac tttacatgaa tgattatttt gatttttcaa tatacattga tgctgaaaat
     1321 aaacaaattg aagaatggta tttacaacga tttaacagtc ttctacaatt agccgaagca
     1381 gaccctagta attactatca taaattcaca caaataccgc cacataaagc tatggaacta
     1441 gctaaagata tttggaaaac tattaattta gtcaaccttg aaaaatatat agaaccaacc
     1501 agaaataggg ctgattttat tattcataaa gggaaacatc ataaaattga tgaaatttat
     1561 ctcaaaaagt gataaagggt tgccaaaaat cacttaatta gatatactta tatagttaga
     1621 ttaatatgga ggtgaaaaca ttggcaaata ttaaatcagc tatcaaacgc gctgaactta
     1681 acgttaaaca aaacgaaaaa aactcagcac aaaaatcagc tatgcgtact gctattaaag
     1741 catttgaagc taacccttca gaagaacttt accgcgctgc ttcttcaagc atcgacaaag
     1801 ctgcttctaa aggtttaatt cacacaaaca aagcaagccg cgataaagca cgtcttgcaa
     1861 ctaaacttgg ttaattaacc tagaaaacat cggatatatc gatgtttttt atttaatttt
     1921 ttcgtagaat aaaagactag gagaaattcc tagtctttta ttctttacta taaacatctt
     1981 caccaaacca tttgtaagag attttttgaa atctcccctt attatgaagc tgtttgaaag
     2041 ctttgttaat cttttcaatt agtctacgat caactttacg agctcctact acaaaatttt
     2101 ctccttgata agcagtttta acaaaataat aagcttttat atttccttct tgctttaaat
     2161 aatagttagc ataaacttca tcaatcaaaa gaccatcaat acggttattt tttaaatcaa
     2221 tcaaagcctg agtgaaagta tcgtattgaa ctgcttcttt tccttttaca aactttttta
     2281 aaatatcagg tttagcgtta aaagcatcaa aaccagatga acccgactgg gctcctagtt
     2341 ttttcccctt catatcctta atactattaa tatgtgatga agttttagta acaattactt
     2401 gatgattatt catatatggg tttgtaaaag cgactttttt agcacgttct gccgtttttg
     2461 aataaccatt ccaaataagg tctatattac cattattaag ttcagtttct ttcatatccc
     2521 agttaatagg ctgccatttc actgaaatac cgtattcttt aaaaacagca ttagctaaat
     2581 caatatcaaa gccggtatag tcaccagaac gactttcaaa tcccatagga acaaaagtat
     2641 tatcaaatcc aatagtaatt ttcttttcct tttgataatg ttcccaatta tcaataccag
     2701 ccatttcctt attagacata ccacatgctg ataatataat cataaataat ccaaatataa
     2761 tggttaataa tatatttttg tgagtcaaca atctaccccc aagtctactt aggattaatt
     2821 ttgagaatcg tatcagatat actttcagca aattgaagat catgtgttac tacaatttgt
     2881 gtcatacctg tttctctatt ttgtaaaatt agtttttcta cttcttgacg caactctgga
     2941 tcaagagcgc tagttggctc atcataacca ataatctgtg gatcaatcat catcgcacga
     3001 gctagtgcga cacgttgctt ttgcccccca gataatgaaa atggatagac taaagcatgg
     3061 tctttaagtc ccaagcgttc aagtaatact aaggcttttt ctttagcaag ttcttttgat
     3121 aatcccatcg ttttaacagg agagaggatc aaattatcta atactgttaa atgtgggaat
     3181 aattgaaagt cttggaagac gaatcctaaa agatttagtg tttccaaatg atcaacagat
     3241 acttctttac catcgtgaac aatagtccct gaatcaatct tttccaatcc agctaacata
     3301 cgtaatagtg ttgtttttcc acctccagaa ggtcctacta aagagagaat tttaccttct
     3361 tctacagtca gattgaaatc tttaaagatt tctttttgcc catagcattt agaaatattt
     3421 tttaattcta acatcgtccc tccttattta tagtagttaa aacgcttttc aacttgttta
     3481 gataaaatag ttaaaagacc aattaataat aggtaaatac cacctgctat aaacattggt
     3541 gcaagtgttg catccctatt tgcggctgtc ttactttcta gaagtaagtc gcccacacca
     3601 agtatgtaaa ccaatgagga atctttcact agagtaatcg tttcattgaa aacactcggt
     3661 aagactattt tgaagacttg aggtaatact atgtaccaaa ccgtttgaaa cttagaaaac
     3721 tttaatacct ttgcagcttc atattgccct ttaggaatag actcaatacc tcctcgaaaa
     3781 atctctgcaa aataagctgc ataattcatc gtaaaagcaa taatcgcagc tggcatacga
     3841 tcaaaagaaa ttcctacact tggtaaaaag taataaaaaa agataagctg taataacaga
     3901 ggcgtgcctc gcataatcca aatataagtt gtcagtagcc attgaagcgg cttaaaatga
     3961 actttcatta ggaaggctac aacaatacca agtggaattg ataaacaaat aacaataaag
     4021 aaaacttgca atgtcacttg cgcacctact aataagctag gtaagatttc taaaatataa
     4081 gacatggtcc cccctaaata aaaaatagat gttctcatta tatcaaaatt caaaccaatg
     4141 tcatagagct tttttaataa aaatgcatga aatcaagtca tattcaaaaa gcaattgacc
     4201 gtcgccaatt gcctgaaagc tattttttta taaactcaga ttttagtttc atagctccaa
     4261 aaccatcaat tttacaatcg atattatgat cgccatcaac taaacgaata tttttaacac
     4321 gagtgccttg cttaatatcc ttaggcgctc cttttacttt taaatcctta ataactgtca
     4381 cagtatctcc atctgataag cgcgttccat tgctatccaa tacaattaaa cctacttctt
     4441 cttcgatttc ttctggattc cactcgtaag cacattctgg acaaaccagt aaaataccgt
     4501 cttcatagac atattcagag ttacacttag ggcaatttgg taatgacatt tttctctcct
     4561 taaattaatt aataaaatag tcagcaagta acattctaac tgattatagg cttcttgaca
     4621 ataggaaaaa gcctattttc cttcaaaaat aggctttttt acaattttta atattttgta
     4681 gttaatatta gcgtatttta gtgtaacgag tctattcaac agttacagct tttgccaaat
     4741 tacgtggttt atcaacatct aagccacgtt gtaatgaagc atagtaagca attaattgag
     4801 tcggaataac cattgaaatg gctgataaga atgggtgaac tttattaaca ataatatcat
     4861 caccttcacg ttccaaccct tcttctacaa taatcaaagc atttgctccg cgagaaacaa
     4921 cttcttggat atttccacga gtatgcgctg caatagttga atcagcagat attaaagcaa
     4981 taacaggagt attgtcttca atcaacgaaa ttgtaccatg ttttaattca cctgcagcaa
     5041 aaccttctgt ctgaatataa gaaatctctt ttagcttcag tgcagcctcc attgtaacat
     5101 aatagtcgtt accacgacca atgtagaagg cattacgtgt cgagattaac aactgctcta
     5161 ccttctcaga gataacatct ttttctgata aggtcgcttc aattgattgt gctacaattg
     5221 acaattcatg aaccaaatca aaatcttttg cttctgcctt accatttgct tctcctaccg
     5281 cttttgctaa gaaagctaac gttgcaactt gagcagtata tgcttttgta gaagcaacag
     5341 cgatttcagg tcctgcatga atgagcatcg tataggttgc ttcacgagaa agtgttgagc
     5401 caggcacatt tgttatagtt aaactaggga ttcccatttc gtttgcttta actaaaactt
     5461 gtctactatc tgctgtttct cccgattgac taagtaaaat aaacattggc ttcttactca
     5521 aaagtggcat gttgtaaccc cattctgaag aaactccaag ttcaacaggt gtatcagtta
     5581 atttttcgat catggtctta gcagcaaaac cagcatgata tgacgttcct gcagccaata
     5641 tgtaaagacg atctgcttct tggactgatt ttataatatc agaatccaca ttcatatctc
     5701 cagattcgtt cgcataagtt gaaatcaact tacgcatcac agttggttgc tcatcaattt
     5761 ctttaagcat gtagaatgga taagtcccct taccaatatc tgataagtca agctctgctg
     5821 tgtagctacc acgttcaatg acattaccat cataatcttg aacttcaacg ctatcttttt
     5881 taacaataac gagttcttta tcgtggattt ccatatattc acttgtctca cgtatcatag
     5941 ccatggcatc tgaacacacc atattataac catttcccaa acctattaga agaggtgatt
     6001 tatttttagc tacgtaaatc gtatcagcat cttgagaatc aataagagca aatgcgtatg
     6061 atccttctat aatatgtaag gcttttttaa aggcttcaag cactgataag ttatcttctt
     6121 caacaaagtg ttcaactaaa tgaatagcta tttctgtatc agtttcaccc ttgaggttat
     6181 gttttgttaa gtaagtttct ttaatttgaa ggtaattttc aataacacca ttgtgtacca
     6241 atacaaaacg accagatcct gaagtgtgag gatgtgcatt tccttcagta ggtttaccat
     6301 gggttgccca acgtgtatga ccgatacctg tcgtaccact aacagaatct cctacttttg
     6361 cctgaagctc cgcgattcga ccgactgact tcacaagttg acttttgtta tcacctacta
     6421 caaaaatacc tgctgagtca tatcctcggt actctagctt ttctaagcct tgaattaaaa
     6481 tatccgttgc atttgtattt ccaactacac ctacgatacc acacatatta ttattaccaa
     6541 taagagcttt ctcttatact ttctatctaa actttctttt tgaattggta tatattaata
     6601 tataccgtta aaaagtaaca aaaacagtat ataattaatg tttttgtttg tcaataaaaa
     6661 aattggtata gtctttttga ttttctgtga taacgagata aacgctatca aaaattctat
     6721 ctatcttaaa aaaatatatt tcttagccat tttatt
//
LOCUS       DATRSG010000078         6593 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRSG010000078 DATRSG010000000
VERSION     DATRSG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.078"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            2..529
                     /locus_tag="VBD89_001799"
     CDS             2..529
                     /locus_tag="VBD89_001799"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO7136302.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            520..1197
                     /locus_tag="VBD89_001800"
     CDS             520..1197
                     /locus_tag="VBD89_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO7136303.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            1329..2372
                     /locus_tag="VBD89_001801"
     CDS             1329..2372
                     /locus_tag="VBD89_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7136304.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGIHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EGIVSGKIQVPMK"
     gene            complement(2463..3362)
                     /gene="galU"
                     /locus_tag="VBD89_001802"
     CDS             complement(2463..3362)
                     /gene="galU"
                     /locus_tag="VBD89_001802"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO7136305.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(3399..4415)
                     /locus_tag="VBD89_001803"
     CDS             complement(3399..4415)
                     /locus_tag="VBD89_001803"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7136306.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVASLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            4585..4914
                     /gene="rnpA"
                     /locus_tag="VBD89_001804"
     CDS             4585..4914
                     /gene="rnpA"
                     /locus_tag="VBD89_001804"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO7136307.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAVKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            4927..5742
                     /locus_tag="VBD89_001805"
     CDS             4927..5742
                     /locus_tag="VBD89_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7136308.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            5750..6571
                     /locus_tag="VBD89_001806"
     CDS             5750..6571
                     /locus_tag="VBD89_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO7136309.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRIETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         2194 a         1023 c         1257 g         2119 t
ORIGIN      
        1 tatggaaaag aaattattaa gaaaagaagt tttgataacc ttaaaatctc aacctcaagc
       61 ttacaaatca gaagttgact ctaaactttt agaggctttt ataaaaacta aagcttatca
      121 aaatagttgt gtaattgcta cttacctatc ttttgactac gaatacaata cacaacttct
      181 tattaaacaa gctttatgtg atggcaaacg tgtccttgtt cccaaaactt atccaaaagg
      241 taaaatgatt tttgtggact atcaaaaaga taacttgagg gcaacaccgt ttggcttatt
      301 agaacctgtt aatgatagag ctgttgaaaa agctagtata gacttaattc atgtgcccgg
      361 acttatcttt aacaataaag gttttcgtat aggttatggt gcaggttatt ttgatcgcta
      421 tttgagtgat tttgaaggtg atactattag tactatttat aggtgtcaga gacaggattt
      481 tgtagaagag aagcatgatg tagcggttaa ggaggtatta tgtctatgaa gaagttcgct
      541 aaagagtatc cgacgacagt actcttggtc ggtctgacaa cccttgtttt tttattgatg
      601 caactaactt atggaagtca agcagagtct agccaagtta tttttcaatt tggtggaata
      661 caaggtgatt atctaaaagc atatccaaca aatctatgga gactaataag tcctatcttt
      721 gttcatattg gttgggaaca ttttttatta aatggtttag ctctgtattt tgtgggacaa
      781 atgggggaaa gtatttgggg aagtttgaga tttctaattc tctatatttt atctggctta
      841 atgggtaata tttttacgct attttttact ccacacgtgg tggcagcagg agcctcaacg
      901 tctttatttg gtgttttttc tgctatagca atcgcaggtt attttggaaa aaatccttat
      961 ttaaaacaag ttggaaaatc atatcaagtg atgatcttgt taaatttatt ttttaatatc
     1021 ttcacacccg gagtgagttt agcaggccat gttggtggtt tagttggtgg agtattggtg
     1081 gctatttttt tgacaaaaca aaatggttct ttattattta aaacttggca aagtatttta
     1141 gctttaatga tatttattat tgtatcgatc agtttaatag gtctatcgtt ggtataattc
     1201 tacggctata gtctctaaaa ccaaactttc tttgggaaaa atagaataaa gtttgctaaa
     1261 tgattttttt atgttatgat agaaatctgt ttttatgtga ctataactta ttaggaggtt
     1321 cattctcggt gaacagaaaa attattggct taactctact atctttatct gtactaacat
     1381 taacagcatg tggtaatcgt tcagataagt ctgcaaataa atcagatatc aaagttgcga
     1441 tggtaactaa tcaaggtgga gtagacgata aatcgtttaa tcaatcagct tgggaagggc
     1501 tacaaaagtg gggaaaaaag aaggggttaa caaaaggtaa tggctttgat tatttccagt
     1561 catctaatga atcagatcat gctaataacc ttgatacggc agcctctagt ggctataatt
     1621 taatttttgg tattggattt ggtattcatg atactattga aaaagtatca gaaaataata
     1681 aagatgttaa atacgttatt gtagatgata tcattaaagg taaagaaaat gtagctagtg
     1741 ttacttttgc agacaatgaa gctgcttact tagctggagt tgctgcagct aagacaacta
     1801 agacaaaaac agttggtttt atcggaggga tggaaggcgt cgttgttaag cgatttgaag
     1861 caggatttaa ggctggagta aaatcaattg atccggctat taaagttgcg gtatcttatg
     1921 caggctcatt tacagatgca gctaaaggta aaacgattgc tgccacacag tatgcaacag
     1981 gtgttgatgt tatttaccag gctgctggtg gtacaggagc aggaattttt agtgaggcta
     2041 aaactgaaaa tgagacccgc aaggaatcta acaaagtctg ggtaattggt gttgacagag
     2101 atcaaagtca agaaggcaat tatgtttcaa aagatggtaa gaaagcaaac tttgttctgg
     2161 catctactat aaaagaggtg gggaaatctc tccaatctgt agcagaatta acagagaaaa
     2221 aacaatatcc aggagggaaa gtaacagttt ttggtctaaa ggatagtggt gtagatatta
     2281 aagagcatca attgtctagt gagggatctg ttgctgtcaa aaaagctaaa gaggggattg
     2341 tttcagggaa aatacaagtt cctatgaaat gataaaagtg tggaggaaca ataagttctt
     2401 cgtcaagtac aataaaaaag tttcctaaat aggaaacttt tttattgtac ttgactcata
     2461 ttttatttct tagatgtttt ttctagagat tttcctaaat caatgatata tttcttcaag
     2521 tcatctttaa cttgtgggtg ttgaagagca taatcaatag aagttttcat aaaaccaaac
     2581 ttgtcaccaa cgtcataacg gtcccctgta aattttctag caaaaacacg ttgcgtttta
     2641 tttaaagtat cgatagcatc ggttagttga atctcatttc ctgcaccagg tttttgagtt
     2701 tctaaaatat taaaaatttc tggagttaac aagtagcgac caatgattgc caaattactt
     2761 ggagcctctt cgggagaagg tttttcaacg aaagtgttaa cgctatatag accattaact
     2821 ccttctcctt gtggagcaat aacaccataa gcagaaacat cttcatgtgg aacttccata
     2881 acagcaattg ttgatgcatg agtagcttca aagtcattaa ttaattgttt ggtgagagga
     2941 ataactttat tattggtaat atccattaga tcatcaccta gcataacaac aaaaggttca
     3001 ttaccaacga aggcttttgc ttgtagcaca gcatctccaa gcccacgagg atgactttgt
     3061 cgaataaaat ggagtcgaat gccagttgtt tcatcaacaa gtttgagaag ttcgtttttc
     3121 cctttctctt ttaggttata ttctaattca aaatttgaat caaagtggtc ttctatagaa
     3181 cgttttgatt taccagttac aactaaaata tcttcaattc cagattttag tgcttcttca
     3241 acaataaatt gaatagttgg tttatcaacg attggaagca tttctttagc taaagcttta
     3301 gtagcaggta aaaaacgtgt tcctaaacca gcggcaggga taactgcttt tctgactttc
     3361 atatatagct cctttaaata gtattaatat aaacgatatt agtgccattc attctctgaa
     3421 cgaaattcat tggacatcat atctaaaatg ctatctttga tattagcccc ttcatatatt
     3481 gatttataaa tagcttctgt aatgggcatg taaacattta agttttgggc gatttcgtaa
     3541 gcaaccttag ttgttgagat accttcaata accatcccca tatttttttc aatatcttca
     3601 agtttctcac cacgtcctaa agcatctccc gctctccagt tgcgagaatg aacagatgta
     3661 ccagtcacaa ttaaatctcc aactccggat aaaccactga aagtgagagg gtcggcccct
     3721 aattgtacac caaggcgtgt tatctcagct aaaccacgcg taataatagc tgctttagca
     3781 ttatcaccat aaccaagacc gtgtagtgct ccagcaccaa cagcaataat attttttaga
     3841 gcaccagctg tttctactcc tacgacatca gtgttagtat aaagtctaaa ataatgatta
     3901 ctaaacaatt tttggacata cttagctgct tcaatatctt ttgaagcagc agtaatcaaa
     3961 gtaatatcac gaacgatggc ttcttcagca tgactgggac ctgaaacaac aacgatatca
     4021 ctacgatatt gttcagagat ttcttcttct aaaattgtag aaagtcgttc atgagtgcca
     4081 ggttctagcc ccttagaagc atgcataatt acaactttat gctttaaaag actagccact
     4141 tgtttagcaa caagtcttgt aactttagtt gggacgacga atagaatgct gtcaacatta
     4201 ttaatcgctt cctcaagatt agtgtaggct tttattttgg aatctaaggt aatatctttg
     4261 aagtaacgtt gattagtatg gttagtatta atctcttcta tttgttcaac cacgtttccc
     4321 cataagcgta cctcatgtcc attgtcattt aatacttgtg caagagcagt cccccatgaa
     4381 ccaggtccaa gaacggcaat tttttgtttt gtcatcttat tccttctttc taataataag
     4441 gcatatagcg ctaacattat atcatactct aatacacttt gtcttgttta gtctgttgtc
     4501 gatgatagta gcattagaaa agaattgagt cttttaggtt atttatggta taataaaacc
     4561 aatttagata aatggagcac tattttgaaa aaaacctatc gggtaaaaag tgataaggat
     4621 tttcaaatga ttttcagcag aggtaaaaat gttgctaatc gaaaatttgt tatctactat
     4681 ttagaaaaag aacaaaaaca ctttcgtgtg ggaatctctg ttagtaagaa attaggaaat
     4741 gctgttgttc gtaacgctgt taaaagaaaa attaggcatg ttttactctc tcagaaaaca
     4801 gcgctgcaag attatgattt tgtcgtgatt gcgcgtaaag gtgtagagga attagactat
     4861 caagcattag aaaaaaatct tatacacgtt ttaaaaatag caggactcat ctagaaaggt
     4921 ataaaattga agaaaaaact taaaacattt agcttaatat tgctgacagg ttctttattg
     4981 gttgcttgcg gacgaggaga agtatctagt cattcagcta cattatggga gcaaatagtt
     5041 tatgcatttg ctaaatcaat acaatggctc tcctttaacc attcaattgg tttgggaata
     5101 atcttattca ctctaataat cagagcgatt atgatgccgc tttataatat gcaaatgaaa
     5161 tccagccaaa agatgcagga aattcagcca aggctcaagg aattacaaaa aaaatatcca
     5221 gggaaagatc ctgatagccg tttaaagcta aatgatgaaa tgcaatctat gtataaagct
     5281 gagggagtta acccatatgc aagtgtatta cctcttctaa ttcagttacc tgtcctatgg
     5341 gctcttttcc aagctttgac tagggtttct ttcttaaaag taggaacgtt tttgagccta
     5401 gaattatcac agccggaccc atattatatt ttacctgtac tggcggcctt atttaccttt
     5461 ttatcaactt ggttaactaa caaagcagct gttgaaaaaa atattgcttt aactcttatg
     5521 acttatgtta tgccgtttat catattggta acaagtttta actttgctag tggtgttgtt
     5581 ttatactgga cggtgtctaa tgcttttcaa gtatttcaaa ttctattatt gaacaaccca
     5641 tacaagatta taaaggttcg agaagaagca gtaagagtgg cacacgaaaa agaacaaaga
     5701 gttaaacgtg ctaagagaaa agcaagtaag aaaagaaagt aggaaaaaca tggtattatt
     5761 tacaggagcg actgtagaag aagctattga aaaaggacta caagagttaa acatttcacg
     5821 attgagggca catataaaag ttgtatcacg tgagaaaaaa ggttttttgg gctttggtaa
     5881 aaagccagca aaagttgaaa tagaaggtat caccgacgaa gtaactgata ttaatgaatc
     5941 ggtagcgtta aaaaatatta agaatgtacc gtctagcgta gatgtggtgg aagaatatat
     6001 agaggaggtt gatgaaacgc ttgaaaaaga agacgtatca caaccagaac ttccaaaaat
     6061 tgatgataaa aacgttgtaa ctacaagtga agctattgaa aaaattgacc tacttcctaa
     6121 tattgaagta gcagctgctc aggtgactaa atacgttgaa aatatcatct atgagatgga
     6181 tttagatgca acaatagaaa ctacgacaag taagcgacaa ataaatctcc aaatagaaac
     6241 gccagaagct gggcgcatca ttggttatca tgggaaagta ttaaaatcgt tgcagttgtt
     6301 agcacaaaat tatcttcatg accgcttttc taagagtttt tcagtatcta ttaatgtcca
     6361 tgactacgtt gagcatcgta tagaaacttt aattgatttt tctaaaaaaa tagctagacg
     6421 tgttttagag actaatgagc cttaccatat ggatcctatg agtaacagtg agcgaaaaac
     6481 tgttcataaa acaattgcaa ctatagaggg tgttgaaagt tattctgaag gtaacgatcc
     6541 taatagattt gtagtagtta ctaagaagta aaaagaggtt tcacctcttt ttt
//
LOCUS       DATRSG010000079         6462 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRSG010000079 DATRSG010000000
VERSION     DATRSG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.079"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            37..993
                     /locus_tag="VBD89_001807"
     CDS             37..993
                     /locus_tag="VBD89_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO7136310.1"
                     /translation="MTNFVCHTLYHLLITIIKLKDKENTRIFICDTITDYETWVKILN
                     DQGIRTESFKEFAYREQLQNKNIEEVMELVDSDLNHYFERVDTQVYLFNDDTLIGRYM
                     VYLGKNYHLIEDGYNCFQAKLFLGGSVVKRVIKTYLFKKYVPYGFSKYCLSIEVNSLV
                     GLPHDIRSKKYKELPRKKLFDSLNKEQKSLIFKIFKTKPLTITPKSVLLLTQPLAQDK
                     CYKTPTERFQSIQEQYDYFDDIVQEYRTLGYNVYLKVHPRDVVDYSKLPVELLPSNVP
                     MEIIELMSTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            990..2390
                     /locus_tag="VBD89_001808"
     CDS             990..2390
                     /locus_tag="VBD89_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7136311.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDTIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2390..3415
                     /gene="neuB"
                     /locus_tag="VBD89_001809"
     CDS             2390..3415
                     /gene="neuB"
                     /locus_tag="VBD89_001809"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO7136312.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3492..4646
                     /gene="neuC"
                     /locus_tag="VBD89_001810"
     CDS             3492..4646
                     /gene="neuC"
                     /locus_tag="VBD89_001810"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO7136313.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETPSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4643..5272
                     /locus_tag="VBD89_001811"
     CDS             4643..5272
                     /locus_tag="VBD89_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO7136314.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5283..>6462
                     /gene="neuA"
                     /locus_tag="VBD89_001812"
     CDS             5283..>6462
                     /gene="neuA"
                     /locus_tag="VBD89_001812"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO7136315.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGHSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     Q"
BASE COUNT         2117 a          958 c         1202 g         2185 t
ORIGIN      
        1 taactatcaa aaagactaat ttaggggaaa aataaaatga ctaattttgt ttgtcatact
       61 ttataccatc tactgattac tataataaag ttaaaagata aagaaaatac tagaatattt
      121 atttgtgaca ctatcacgga ttatgaaact tgggtaaaga tattaaatga tcagggaata
      181 agaactgaat catttaaaga gtttgcttat cgggagcagt tacaaaataa aaatattgaa
      241 gaagtgatgg agcttgtaga ttcagactta aatcactact ttgagagagt agatacacaa
      301 gtctatctat ttaatgatga tactttaata ggtcgctata tggtgtattt aggtaaaaat
      361 tatcatctaa ttgaagatgg ttataactgt tttcaagcca agttattctt gggtggttct
      421 gtcgtaaaac gagtaattaa aacttactta tttaaaaagt atgttccgta tggtttttca
      481 aaatattgtc tctcaattga ggttaattct ttagtggggt tacctcatga cataaggagt
      541 aagaaatata aagaactacc gagaaaaaaa ttatttgata gtcttaacaa agaacaaaaa
      601 tcactgattt tcaaaatatt taaaacaaaa ccattaacta taactccaaa gtcagtatta
      661 ttgttgacac agccacttgc acaagataaa tgttataaaa cacctacaga gaggtttcaa
      721 agtattcaag agcaatacga ttattttgac gatattgtcc aggaatatag aacgttaggg
      781 tacaatgttt atttaaaagt tcatcctaga gatgtagtag attattccaa attgccggta
      841 gagctattac catcaaatgt tcctatggaa attatagagt tgatgtcaac aggtcggttc
      901 gaatgtggga taacacattc gtccactgcg ctggattttt taacttgtgt tgataaaaaa
      961 ataactttag tagatctgaa ggatattaaa tgaatactaa gaaattactt caatctggtt
     1021 ttatttatac actcgggaat ttattggtgc aaggattagc ctttatcaca cttcccatct
     1081 atactcgggt tatctcaaca gaagtttatg gccaatatag tttatatgtc gcttggatga
     1141 atattattat gctttttatt ggccttcaga cgagcggttc tttaagttca gcccgggtca
     1201 aatacggaga agaatttaaa agctattcag ggagtgcctt ttctgtaggt aatatatggt
     1261 ttcttattat acttttgata gcttttctat ttagaagttt tcttgcacca ttagttggtt
     1321 tttctgaatc tattttttta ttaatggtgt gtcaaagtta cgctagctat gtggtgactt
     1381 tctttggtca gtattttata caacaacaga ggagtttggc taatttaata ttatccttag
     1441 ccaatgcagt ttcatctgtt gcactatctc tatttttaat ttttcattgg tccgatgact
     1501 ttttatctag ggtttttgga gcttttgttc ctactataat aactggaata gttgcctttg
     1561 cttatattta ttatcatagc aaatcttttt acaatcctaa gtattttcgg ttcattgtca
     1621 ctgtgtctgt tcccttgatt ttccatttgt taggacacca gttgttgggg caattagata
     1681 gaattatgct agctcgttta tacaatacta aagaagttgc aatgtatagt ttcggttatt
     1741 cgcttggaat gattattcag attgttttga atagtattaa tatggcttgg attccatggt
     1801 tttttgatgc tagaaaagaa aaattactgc aactttcaac ttatattagc cgttatctct
     1861 acttaggagt tttcttaaca ttaggttatt tgactgtttt tcctgagtta gctcaaataa
     1921 tgggaggaga caagtatagt agtagtgttc aatttatttc tttaattatt gtcagctact
     1981 ttttagtttt tctctataca tttcctgtaa atatccaatt cttctatgct aatacgacat
     2041 ggattcctat tgggacatta ttagctgctg gtgtaaattg gttactaaac cttgtattaa
     2101 ttcctcatta tgcagcctat ggcgctgcta tggctacaat aatatcttat ttagccttgt
     2161 tgatttttca tcatattgtt tcgaaagtta aataccatta ttcagatgtc tcagttaggc
     2221 agtacatcat tttaagtggt atagtattta gttatgctat gttaatgaac atgttcctag
     2281 atactatcgt tattagatgg agtttaggaa taattattct tatagtatat agcattgttt
     2341 ttcaaaaagt cattttagac ttattgagta aaaaaagaag gagaagataa tggtttatat
     2401 tattgcagag attggttgca atcataatgg agatattaat cttgcgaaaa aaatggtaga
     2461 tgttgccgtg tcttgtggtg ttgatgctgt taaatttcag acttttaaag ctgagaaact
     2521 tatttctaaa tttgctccca aagctgaata tcaaaaagca actacaggaa cagcagacag
     2581 tcaacttgag atgacgaaac gtttagagtt aagctttgaa gaatacttag aaatgcgtga
     2641 ttatgcaatt tcaaaaggtg tggagacctt ttcaacacct tttgatgaag agtcattaga
     2701 gttcttaatt tctacagata tgccaattta caaaattcca tcaggagaaa tcactaattt
     2761 accttactta gaaaagattg gcaagcaaca aaagaaagtt attctttcga cgggtatggc
     2821 ggtaatggaa gagatccatc aagcggtgaa tattttacgt cagaatggta caaccgacat
     2881 ttctatttta cattgtacaa cagagtaccc aacaccttac ccctctctaa atttaaacgt
     2941 tattcatact ttgaaagatg aatttaaaga tttaacgata ggttattcgg atcattcaat
     3001 tggatcagaa gtacctatcg cagcagcagc aatgggtgca gaagttattg aaaaacactt
     3061 tactttagat actaatatgg aaggtccgga tcataaagcc agtgcaacac ctgatatttt
     3121 agctgcttta gttaaagggg ttcgcattgt tgaacaagcc ttaggtagat ttgaaaaaat
     3181 cccagatcca gtagaagaga aaaataagat tgttgctcgt aaatcagtcg ttgctttaaa
     3241 accaattaaa aaaggcgata tttattcaat agaaaatatt acggtgaagc gcccaggtaa
     3301 tggtatttct cctatgaact ggtatgacat cttgggacaa gaagcgcaag atgatttcga
     3361 agaggatgaa gttattcgtg attcacgctt tgaaaatcaa ttgcccgagt tataataaca
     3421 cctaattata atcggcaaca gatgtattac acggctaaga ttatgtaatt actatatata
     3481 gaagggatac gatgaaaaaa atttgtttag taaccggatc gcgtgctgaa tacggaataa
     3541 tgaagccatt gattcaaaga ttatcaaaag ataaagaagt caacttacaa attattgcaa
     3601 cagcaatgca tctggaagaa aagtacggct atacttatcg tcaaattgaa gaagacggtt
     3661 ttgatattgc ttataaagtt cccttacatc tttatgatac tgacagaaga actgtatcta
     3721 ctgcaatggc gcatttacaa ctaggattga ccaaaatttt tgacaaggaa gactatgatc
     3781 tagtcatcat tttaggggat cgttacgaaa tgttaccagt tgtgaatgta gcgttgattt
     3841 ataatgtccc agtatgccac cttcatggag gggagacctc attaggcaat tttgatgagt
     3901 atattcgcca tgcaattact aagatgagtc acctacactt agtctctaca gaggactttc
     3961 gtcaacgtgt gattcagatg ggagaacaac ctcaatttgt aattaacaca ggagctctcg
     4021 gagtggaaaa tgctctatca atcccttctc taaccaaaga agcaatagaa aagcaattag
     4081 gtattgtact agaggaatcc tattttgttg ttctctatca tcctgttact tttgaacaag
     4141 gaaaaagtgc tggagaacag atgaaagcgg tactatcagc cctttctaaa tttggtgtcc
     4201 agtgtctatt tataggatcc aattcagata ctggttctga tgatattgca aaggctatca
     4261 atacttattt gataaatcat gaaaatagtt attgctttgc ttctttatca acccaacttt
     4321 atcattcctt aattcgtcat tcactaggac tgattggtaa ttcgtcttct ggtttaatag
     4381 aagttccttc tttgatgaaa cctactctta atattggtga tcgccaaaaa ggtaggctac
     4441 atggagaaag tgtagtaagt gtaccagtcg aaaccccctc tgtattggag ggattatcaa
     4501 aattgaatga agtgaccaac tttgacaatc cctactacaa agagaatgct tcatctattg
     4561 catatgaagc aataaagctt tatttgaaag atgaaccttc aatttcccaa ccattttatg
     4621 atttaaagga gaataatcta aaatgaaaaa aaagttggca tttttaggcg caggaagtca
     4681 tgctgatgcg attgctccgg ttattgatcc tcttgtgtat gatttcgtag gtttttttga
     4741 tgataaagat attacggagc atgatggtta tcctgttctt ggaaaacttt atgatgtgct
     4801 accttacctt gaagatggct caatagatgc agtatttatt acaataggtg ataatgctaa
     4861 aaggaaagaa ctatttgaat atgtagcaaa ggattattat gactttatta ttaacatcat
     4921 tagtcccaat gctttagtat tgacaccaga tagtatttgt gggcgtggta tctttattgg
     4981 ttttggggct tttataggtt ctaaagtgaa gctgtttgat aacaacgttg ttaatacagg
     5041 agcgctcatt gaacatcata ctgttgtaga atcacactgt aatatagcac ctaatgctac
     5101 cataaatggt ctttgttata ttagagaaga agtttatgta ggtagtgcca gtgttattat
     5161 tcaaaccttg gatatttcat cgtgtacgac aattggcgca ggagctgttg ttgtaaaaga
     5221 tattatagaa cctggaacct atgtaggggt tccagccaaa aaaataaaat aaaggatatg
     5281 ttatgaagcc aatttgtatt attcctgcgc gatcagggtc aaaaggatta ccagataaga
     5341 atatgttatt cctagcaggt aaacccatga ttttccatac tatagatgct gcaattgaat
     5401 ctggaatgtt tgataagaaa gatatttttg ttagtacaga ttcagaattg tacagagaga
     5461 tttgtttaga acgcggtatt tcagtggtga tgagaaaacc ggaactttca actgatcagg
     5521 caacttcgta tgatatgtta aaagattttt tatctgacta tgaagataat caggagtttg
     5581 tgttacttca agtaacctct ccactaagaa aatcatggca tataaaggaa gcaatggagt
     5641 attattcttc acatgatgtt gacaatgttg taagtttttc tgaagttgag aaacaccctg
     5701 gtctgtttac gacattgtct gataaaggct atgctataga tatggtggga gcagataaag
     5761 gttatcgtcg ccaagattta caacctttat actatccgaa cggcgctatt tttatttcta
     5821 ataaagaaac ttacttaagg gaaaaaagct ttttcacctc taggacatat gcttatcaaa
     5881 tggcaaagga attttcatta gatgttgata cgagagatga ttttatccac gtcatcggtc
     5941 acctcttttt cgattatgcc attagggaaa aagagaataa agttttttat aaagaaggct
     6001 atagtcgttt gttcaataga gaagcttcaa agataatttt aggtgattca aaaacgatat
     6061 ctatctcact agaaaattac cataattatt cccaaggtgg tgtaacatta gcaacgatgt
     6121 tggaaaactt acctaacttt ttgacagcta atgtaacaga ggcttttgtt tctatagggg
     6181 taaatgatct cattacaggt catagtgtgg aagaaatatt cagcaatttc caaaaactct
     6241 actcattatt agctgagaat aaaataaaga tgagattgac aactattgct tacacccttt
     6301 ttagagaaac tgtcaataat gcagatattg agaaaatcaa tcaatggcta acagaatttt
     6361 gttatcaaaa tcagattcca ctgttagata ttaatagatt tttatcaaag gatggtaatc
     6421 taaattatca tttaactagt gatggattac attttactca ag
//
LOCUS       DATRSG010000080         6367 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRSG010000080 DATRSG010000000
VERSION     DATRSG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.081"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(344..2320)
                     /gene="ftsH"
                     /locus_tag="VBD89_001813"
     CDS             complement(344..2320)
                     /gene="ftsH"
                     /locus_tag="VBD89_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO7136316.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
     gene            complement(2343..2885)
                     /gene="hpt"
                     /locus_tag="VBD89_001814"
     CDS             complement(2343..2885)
                     /gene="hpt"
                     /locus_tag="VBD89_001814"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO7136317.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            complement(2890..4164)
                     /gene="tilS"
                     /locus_tag="VBD89_001815"
     CDS             complement(2890..4164)
                     /gene="tilS"
                     /locus_tag="VBD89_001815"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO7136318.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNIYFPTLERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKDREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            complement(4166..5452)
                     /locus_tag="VBD89_001816"
     CDS             complement(4166..5452)
                     /locus_tag="VBD89_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7136319.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIISLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            complement(5452..5586)
                     /locus_tag="VBD89_001817"
     CDS             complement(5452..5586)
                     /locus_tag="VBD89_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO7136320.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            complement(5589..5960)
                     /locus_tag="VBD89_001818"
     CDS             complement(5589..5960)
                     /locus_tag="VBD89_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO7136321.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            complement(5947..6219)
                     /locus_tag="VBD89_001819"
     CDS             complement(5947..6219)
                     /locus_tag="VBD89_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO7136322.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
BASE COUNT         1937 a         1210 c          913 g         2307 t
ORIGIN      
        1 tagcaaactt attctgcttt gtcaaccact ttttttaagt tgttaactac gcgctactag
       61 aaactgctct ctcgagacaa cttattcatt atactaaata tttctacttc ctgtcaatac
      121 tatttttgca ttttttcttt tatttttaaa aagttaatat tatttatagt aactaacctt
      181 ctatacttgt ttaatggata gcatttttac cgttgtcatg ttcatatttc atcttcttaa
      241 ttcacaaatt taaacttcat cttcataaaa aacacccctc aaattttatc taaatttgaa
      301 gggtgtttga aatttataaa gttctttaaa aatatatgat gacttatttt ttatcttctt
      361 cttgcatttt ttctttgatt tcatcgtatg ataacgctct cgctttatct tcatcatttt
      421 ctgtctcagg cattttacct gtctcgaaaa tcgatttaat ctgagcagca tcaagagtct
      481 catattttaa gagggcttct gcaattaatt tatgagtatc acggttttcg ttgataatat
      541 cagctgcctt attacgtgct tcattaagaa ggtgacgaac ttcatcatca ataagttgtg
      601 cagtttgagc agaatatgat ttttcaggtg acatttggcc agccatcatt gcatggttac
      661 cttcgtattg aactgggcca agtttctcac tcataccata ttcagttacc atagcgcggg
      721 ccatggcagt ggcttgttca aagtcatttg aggcacctgt tgtctgagcg ttgaaaataa
      781 tttcttccgc tacacgtcct cccataagac ctgctaattg ctctttcata tcatcttttg
      841 aaagaagcat ttgatcttct ttaggtaaag caatcatata tccacctgca cgaccacgtg
      901 gtacgatagt aactttatga acaactcgcg catttgaaag aatcaaaccg acaattgtgt
      961 gcccagcttc atggtaagca accatagctc tttctctttc agaaatagta cgatcttttt
     1021 tagaaggacc agcaattaca cggtcttctg cttcatcaat atctgaagca tcaataactt
     1081 ttttatttcg tcgcgcagca actaaagcag cttcattgag aacattctcc aaatcagcac
     1141 caacaaatcc tggggtttgt tgagctacta cttttaagtc aacattatct gctaatggtt
     1201 tatttttagc atgtactctc agaattgctt cacgaccttt tacatcaggt tgtccaacta
     1261 gaacttttct atcaaaacgt cctggacgca gtaatgcggg atctaacacg tcactacggt
     1321 tggtagcagc gataacgata atactttcgt ttccttcaaa accatccatt tcaattaaaa
     1381 gttggttgag cgtttgttca cgttcatcgt ttccgccacc cataccagct ccacgtcgac
     1441 gtccaacagc atcaatttca tcaatgaaaa taatagcacg ttcagctttt ttagcatctt
     1501 caaacaaaga gcgtactcga ctagctccaa caccgacaaa catttcaaca aaatctgaac
     1561 cggaaatact aaagaatgga acccctgctt caccggcaac agcttttgct aatagggttt
     1621 tacctgtccc tggaggtcct tctaatagaa cccctgctgg aattcgggca cctagagatt
     1681 tatatcgttt aggatcttta aggaagtcaa cgacttctat taattcttgc ttttcttcct
     1741 ctgctccagc aacatccgaa aatcgaactt taacctcatc tttactagat gatcttgctt
     1801 tatttttacc aaagctcatg gcaccacgag cgccaccgcc accgccttgg ttcatcatca
     1861 tcataaagaa accaatcatg ataactaaag gtaaaaaact ggcaatataa gaaatccatg
     1921 tcccacttga actttcatgt ttgacctgaa tattagtgtt attttcttca gcagcgctaa
     1981 caagagattt tattgaagag tcatttggta gtataacaga attaaatcct gtaactttag
     2041 tagcaacact accacctaaa aaagtaaaac tattagctga tttaattgtt ttggctttct
     2101 tatatgtccc actaacttca acaacaccac cacttggctg gtatgaaata gacttaattt
     2161 ctccagcttt taattgttta acaagttttg tataagaaat ttgctggttt tgagagctag
     2221 ttccttttaa atagtattga aaggttgtaa taaccgcaat aatcaataat atgtaaataa
     2281 aggaattttt cagaaaacca ttatttttat tatttttcat agtttaagag agtacctttt
     2341 ttctattttg agtaaatttc ttcttttaac acaccgacat aaggtaggtt acgatagttt
     2401 tcagcatagt ctaaaccgaa tcccacaata aattcatttg ggatatcgta acaaacgtaa
     2461 tcagcatcaa tatctaccaa tcgcccttct ggcttatcaa aaagggttgc aactttgaca
     2521 gagttagctt gacgatactt aaacatatct cgaaggtatt ttaatgtacg tccagtatca
     2581 atgatgtctt caataaagat tacatctctt ccttcaatgt tagtatcaac atcttttaaa
     2641 attttaactt ctccactact agttgtacca ccatgatagc ttgaaacaac cataaaatca
     2701 atttcaacat gtgtatcaat atgctttaat agttcagcca taaaaggcac tgaccccttt
     2761 aatacaccta ctaataatgg attcttacct gcataatctg cagttaattt agctcctaat
     2821 tctttcgttt tcagaataat atcttcttct gaatataaaa ctttttttat atcattttca
     2881 agcatgatgt cacctttttt ctaaattttc aatataaagt ttgcccttca ttatatcatg
     2941 ttcagaaggt tttctcaaat atgttctacc tgcaacaatc acaaatatca actctttatt
     3001 ttgctcccca ataacagctt cttctctgtc ttttagagtt attttctcat caataaatag
     3061 acggcgaatt tttttagtgt gatttcctaa aaatatccta tccccttctt ttcttcttct
     3121 taaaataatc ggagaagtat cataaatagg aatagtaatg tctgcttgag aaatgaattt
     3181 tccataggaa aataaatatt gtgagaatat ttgtatatta tgcaattcta atcttttttc
     3241 aaccttaaaa ctatccgtct taggatggat ttttgtaata tgaaaactct cgtaatcctt
     3301 atgaagaaaa tagcccgatt taatagtata gtcaatattg gctttagttc taatcaattt
     3361 taatattact ctgaactgtt ctttactcac ttgtaaatct ggaaatttgc taatgtaatc
     3421 ttgcaataaa aattcttgga cggaatgagt ttgccttaaa aaatcatagc agtttgttac
     3481 ttcgatgctc cttgttaggt ctgttaaagc cgtgaataaa cactctgttt cctcagagag
     3541 agttatgaga gaatctttca attgattatt ttctctttcc aaagtaggaa agtagatgtt
     3601 tcttattcga tttctcaaat aatcagacga agcattgcta gcatcttcaa aatgaaaaat
     3661 atttggtaat aactcctttt tatatggaag aaatggtcta atcaattgac catttgcaaa
     3721 agcactcact tgcttaatcc ctgataaata gcgtagcctg cttcctctta aaattctcat
     3781 aaatacagtt tcagcttgat catcagcatg gtgagctgtt actaaagccg tgtaaccttc
     3841 ttcacgcata acttttgaaa aaaaattgta gcgatggtta cgtgctctat cttctgaaaa
     3901 tattccttga aaataagaga taaagacagg gacatcatgt atctgtcccc actgtctgat
     3961 atattcttct tctttttctg attcttttcg ctgtccatga tttatgtgtg caataccaat
     4021 actaattgat aactcttttt gatactggta taaaaattgc aacaaattta ttgaatcaat
     4081 gccaccagaa acagcaatta gaactttctg atgagcggta aataaccctt tggataaggt
     4141 atctttcaaa atagtgttat acatattact ttaaaacctg gtaaacgtcg tcagcaatct
     4201 tagtgatatc atcgtaagat gatttatctg taaaaataat cataataaaa ggttgttctg
     4261 cataaacaat agccgcatca tgtttgtagt cataagcatc tccaatttta tgtgctactt
     4321 taactggtat atttttaggg attcttgtat tatcaaaatc agtctttgat aggtaattaa
     4381 caatatctcc attttgataa taaattgctt ccatcatttt acccgcagct tgtggagaaa
     4441 tctggcgttt cttcatatcc cagtgcatac cagaaattgt gtctacttgt gtttgaaaca
     4501 ttgaatcgta ttgattattg acataataac ctagcatatt tgttgcaaca ttatctgatt
     4561 ccttggcagt tgcttcaagt aactccttaa ctgtatataa tttatgatca gcttttttct
     4621 ctaatttccc actaccactt ggatcatagc tattaggaaa ttgattaaca ttatctttgt
     4681 attcaaattt gctgtccaat gacagttttc ctaattttac ttgctcttgg ctagcataaa
     4741 gtgttgctaa ttttgcaata cttgcagagt acattgacct atcaatatta ataccagcta
     4801 ctgtctgtgt atttaattgt ttgatgtata taccatattt atctttatta tatttttcca
     4861 ataatacttc ttgaacctta gccatacgat tatcagcaac cgatagatct tctactgata
     4921 tccaaaaacc actgtccgtt ttataataaa taccatgttc tgtttctgct tgttctacta
     4981 aatgcactct ggaataaggt tttaaattat ttttaacttc actaacacca tttgtgtaag
     5041 gtgcttcata agtcacaaaa gaaggtttta accaataatc caatgagaca gattgctgct
     5101 tagaaactaa atcattaaaa atagcttcac gactagcctt tatgaactgt ccattggaca
     5161 aagtaaatac tggaattcca tttgaattaa ttgtaaggga aataatcgac aataaagtat
     5221 ttggaactaa agtctctcct gttatggtta aagcttcatc ttgaaaactg cttatttcaa
     5281 tttttgtagt tggtctctct ggaatagcat caaaatacat ttccttatcc acaactgtct
     5341 gagttaaggc gtatcgtcta ttttgagaaa gcgttaatgg ctcctcggta cttatagctg
     5401 ggattgatac aaaatataag ctacataata agaaggttaa aactttcttc attacttttc
     5461 tctgctctca atcttttctt tttctacaat tgtttgccta agttgatagt tcttatcagc
     5521 taactcttct agtttgtcat ccgagtaggc tagaaaatgt tctattgttt caattaaatt
     5581 attcataatt attttggtaa aaggtctggt aatgggtaaa tcatttcgcc ggtcttagag
     5641 aaataatact tcgctcgagc atatttttga acgtaatctg gattttttag ttgttttgct
     5701 agtaacttct ggttctcagt tctattagtt aatgtctgat agtctttcgt taattttaca
     5761 acttcttgac gacgttcttg taaagttctg taactcttaa ctaaattata agtgggtaaa
     5821 ataaataaaa gcatgacaaa aataagaacc caacccatta aacgattttt tcggcgtaac
     5881 tcctcagctt cgtaacgttt ttttagattc tcatcgttaa tatattgatt atttaactga
     5941 acaacattag gcttgctcat ctgtttctat ccttgtttca ttaataattt catacatttt
     6001 gatagcatct tctttcttag tgctatcttt catttctaat actttaacgg tcaacaattt
     6061 attaccaaaa cgaatttcaa cttggtcatt aagctttagg tcagttgatg actttgctaa
     6121 tacgccattc accttgacac gtcctttgtc tgcaacttct ttagcaacgg gacgacgctt
     6181 aatgatacgc gacactttta gatacttgtc taatctcact gatgatcaca tccttctatc
     6241 taaattaact ctactatttt agcatttttt ttgaaaaata tctgaactga taatatttgt
     6301 caatataatg taacaaaaag agagctatta gctctcttaa ttctctcaaa tatcaagtaa
     6361 aaaactt
//
LOCUS       DATRSG010000081         6347 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRSG010000081 DATRSG010000000
VERSION     DATRSG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.082"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1369)
                     /gene="gtfA"
                     /locus_tag="VBD89_001820"
     CDS             complement(<1..1369)
                     /gene="gtfA"
                     /locus_tag="VBD89_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO7136323.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLD
                     RSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFIT
                     STETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKH
                     IDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYK
                     DYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNP
                     QQISTA"
     gene            complement(1385..3781)
                     /gene="secA2"
                     /locus_tag="VBD89_001821"
     CDS             complement(1385..3781)
                     /gene="secA2"
                     /locus_tag="VBD89_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO7136324.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKVYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKDLTEHSLRRYILENLTYKFKYFPDEFDV
                     HNSDDVFKLLIDIFDREFTAKKTKLQSDNEFDNFVRISILKAIDKSWIEEVDSLQQLK
                     GVVTNRGIGQRDIIQEYYKESLNSYYKMGKEIRYSIVKNIMLSTISKASDGSYSIYYV
                     "
     gene            complement(3778..4323)
                     /gene="asp3"
                     /locus_tag="VBD89_001822"
     CDS             complement(3778..4323)
                     /gene="asp3"
                     /locus_tag="VBD89_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO7136325.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQINFYNRQKEKIGF
                     SIIRDHSSDFIVPDDTYFYDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVE
                     SSLPSELELIRTLIAFSKDKK"
     gene            complement(4316..5905)
                     /gene="asp2"
                     /locus_tag="VBD89_001823"
     CDS             complement(4316..5905)
                     /gene="asp2"
                     /locus_tag="VBD89_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO7136326.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRPDDFETSMDILQNIIGSQNDEAINQFNQKFWN
                     KFKRSNFEKTQFAISYMKHDDYDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQSIN
                     NWFTSQYKTILSDDFGRVFSD"
     gene            complement(5907..>6347)
                     /gene="asp1"
                     /locus_tag="VBD89_001824"
     CDS             complement(5907..>6347)
                     /gene="asp1"
                     /locus_tag="VBD89_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO7136327.1"
                     /translation="DKILSSKNPDYFFTSLSLEDENHFKEEQKTKIRGISFDIYTNEN
                     QIIDALDTARLVIDLRDDPDLYTQIAALSSGIPQINKVHTNYVEHQKNGWIISDSTEL
                     NNAIDYYFDGLMNWNKSLVYSVKKMGDYTSGKILQRWRKLLEKE"
BASE COUNT         2047 a         1212 c          838 g         2250 t
ORIGIN      
        1 aagcagttga tatttgttga ggattatctg gtgtttccct tggaataagg tagccattca
       61 aattattttc aataaaggtc tgattaccat acggaacatc taaaccaatt atcggaagcc
      121 ccgatccgat tgcctccatc aaggttaaac caaaaccttc actggtagaa cccgataaat
      181 aaacttgata atctttataa acagaagata ggtttttatg ccccattaat cgtatataat
      241 tatttgcctt atttttagca attatttctt gtaacttttg ccgttcgcca ccttcaccat
      301 aaatatcaaa ggttaattca ggaattactt tattagcttc taccacagca ttaattaacc
      361 aatcaatatg tttttccgaa gctagacgtg agcaagttaa caacgaaaaa gcttttcttt
      421 catgccactc ttttctctgt aattttgata aacttcctac aggaattgta taaacgaccg
      481 gttcaatatt taaatacttt ttaaattgat caatcaacgt cttagtttgc gtttctgttg
      541 aggtgataaa agcatctatg ctatcagcat ttgaaaattg atagtcataa taattattcc
      601 ataaaatcgt agtttcatca gttgcgctaa cattataatg ctctgcatga acaacaacag
      661 ctactttagc atttccttta ttccttagaa cagcttgccc aatgcctgta gagcggtcta
      721 acaagattag atcactattc gttaattgta atttttctaa catgtatcca actaattctt
      781 ctttagaata tagtatagtt tctttgaatt taaaaatact attctcgcca tctacatttt
      841 cttcatatgc aacacttcca tcttcattat aaaatgtacg ctgatacagg catggaccta
      901 tatcttcggg agcataatat tctgaaaaca ttttagtata tgagtagtag tcttttctaa
      961 caagctttcc ccccataaga tactctaccc tttggacgat gttactagat tcatcgttaa
     1021 gcataactgt aactacaata ctacttcctt taaaaaaaaa tgatattatt ttaccgtttt
     1081 tctcagtcct atcaatcggt agtgaaaaac tttctttcaa ttgttgcagt gaataagaag
     1141 ttgcagcaat tgttaaatca gtaaaaaaag tataaagcca tataatctca ttgtcttcaa
     1201 agccgatatt tcgagtaagg tgctctatat tttcattctg aaacatatca gtaaaaataa
     1261 atttagcttc aacacctaac tttctaaaaa cttctgaacg atatgcttga gcatactcta
     1321 caccgctact tgcccaacct atccctctat tcaaattata aactaccatt aactccgttt
     1381 tcctttatac ataatatatt gagtaacttc catcacttgc ttttgaaatt gtacttaaca
     1441 taatattctt aacgatagaa tatcttattt cttttcccat cttatagtaa gaattaagcg
     1501 attctttata atattcttga ataatatctc gttgaccgat acctctattg gttacaactc
     1561 cctttaattg ctgtaggcta tccacttctt ctatccacga tttgtcaata gcctttaata
     1621 tcgatattct gacgaaatta tcaaattcat tatcactttg aagtttagtt ttctttgcag
     1681 taaattctct atcaaatatg tcaatcaata atttaaatac atcgtcagaa ttatgaacgt
     1741 caaattcatc agggaaatat ttaaatttat aggtaagatt ttctaatata tatcttcgta
     1801 aagaatgttc cgttaaatcc ttatcattag caatatcgtt aaaattatcc tgaataattt
     1861 ttatcacacc atcaaccaca atggaactat cagctattaa ttcatttcgt aattgataaa
     1921 tattttcacg ctgtatacgc aagctctctc cgaacttaat agtttgctcc ctagaagata
     1981 cacctttatc ttcacttctg agttgagcat ttttaactat ttgataaaaa aagcgtttct
     2041 ttctaggtac catatagtaa tttgaagttt ctttacgaga atattttttt aaatacttta
     2101 tcacccattc cggagaatga gaaacaatta attcatcctc tagcgacaca taaaattggc
     2161 taatccctgg gtctccttga cgccccgaac ggcccctaag ctgccagtcc atacgtaaat
     2221 taggcatcct ttccgttcct ataactgcaa gtcctcctaa ttctctaacc ccttcaccta
     2281 gtttaatatc tgttccacga ccagcaagaa ctgttgcaca tgttactgaa cctttttgtc
     2341 ccgcttcttt tataatttgt gcctctttaa caacattatc agctgttaag gtattatggg
     2401 caatgccttc tcgcagcaat aactttgaat aaatgttagc aatttcaact gttccagaaa
     2461 ttaacaatac tggctgacct ttactatgtc tttgtttaac atcatctata gtagcttgca
     2521 acttttctgg taagctaaca tatattttat cggcaagatc tactcgtttg atcggaaaat
     2581 ttgttggtat aacaataaca ggcaggtggt aagtttttat aagttcatct tcagaagatt
     2641 ttccagttcc tgtcatccca gacaaccttg ggaacatatt aaagagactt tgatacgtta
     2701 ttgaagcaat tgccctagat tcatttgtca aagcaacatc ttcttttgct tcaatagctt
     2761 ggtgaatccc tgattgaagt tttgtgcctt ccataacacg acctgttgcg ttatctaaca
     2821 attttacttc attattttta actacataat cataatctct tttatacaaa caatgagcac
     2881 gcaatgctag gtttatatgt ctatttaatt cccaatactc atcattatat aattctgaaa
     2941 tattgaagta ctgttttgcg taacttacac ccttctcagt aagatagaca attttatcct
     3001 tagctttatg gatatattca tcattttcct taagagtgag tacaaaatta ttagtgatac
     3061 catagagatt agattgtaca cgaggatctc ccgctataat taagggtgtt tgagcaatat
     3121 ctaacaaaac agagtctgct tcatcaacga taacaaaatg aaaaggtctt agaaattgtt
     3181 ctagtttatt actagccaga ttgtgaacga gatagtcgaa acctaaggca cctgcagtag
     3241 tataagtaat atcagattca tatacttctc tcttaatagt cgcatctgct tcttcttttt
     3301 tatcaaaaac attaatacca actgtcatac ctagaaattg atatactttt cccatctcta
     3361 atgcatctcg ttttgccaaa taatcattgg tcgtcactaa gattgctccc ttgctagtta
     3421 aagcattcag atacaagggc atagttgcag ttaatgtttt tccttctccc gttttcattt
     3481 cagctatcgt tccttgatga agagcaatag cacccataac ctgagcatca tatggaaaca
     3541 aacctaaaat tcttttatta gcctctctta tcacagcaaa agcatcaaca agaatatcat
     3601 ctagactttc tccacttgaa agtctatttt ttaatattaa agtttgatgg cttaactcct
     3661 tatctgataa ttgagcgtag tacacttttt tattatttat ctcgtttaca attttttgaa
     3721 ttctttgcaa tctcaattta tttagaatag agattttggg tttaacaaca acttttttca
     3781 ttttttatcc ttagaaaatg ctatcaacgt tctaataagt tcaagctctg atggtaagct
     3841 gctttcaaca taactgttta gttcaatacc atctttttct tcttgagtct caattacttc
     3901 aaataattct aggtaacaaa attctacact tatacaaccc gcaccaataa gagaaatatc
     3961 ataaaaatat gtgtcatcag gaactataaa atctgaggag tgatctcgaa taatagaaaa
     4021 acctattttt tctttttgac gattataaaa atttatctga ataatcacgc atccctcagg
     4081 cacagtagtt aattttgata ccaactcata ggtatgatta ggttttagta aggtaagctc
     4141 tggcaacgtt ctattagctt gaaaattcgt tttagaaagc cattttttga ttgtttttcc
     4201 tgtagagaat cttagattct caaaaaaaac acgattatgc tccaaatact ttatagtcga
     4261 accataaaga taaacattgc tactatcagt ccaaagcacc ttctgtattc tttttttaat
     4321 cactaaacac tctcccaaaa tcatcactga gaattgtttt atactgagaa gtaaaccaat
     4381 tatttattga ttgactatta tcattatgtc gcccagcata tccacttgca tacaacaggg
     4441 tgttagtttt agaaaaataa tcaatcagcc ttggagttgc atttctatca taatcatcat
     4501 gtttcatata agatatagca aactgcgttt tttcaaaatt agaacgctta aacttattcc
     4561 aaaatttttg attaaattga ttaatagctt catcgttttg agaaccaata atattttgga
     4621 gaatatccat actcgtttca aaatcatctg gtcttcttaa ttttaaattt actaccatat
     4681 cgcctaaatt tgtaaagggt ttccccacaa ctatagcatg gggggaaaaa gtagaaccat
     4741 aataaagggc accaaaactt cccatagata aacctgataa aataagttga caatgatcaa
     4801 attgtaggta atctaaatat ttttgtaaaa cattttctaa ttcactttcg tattctgaag
     4861 tacctaggta gaagcttcca ccttcaatcc ttggatcacc aattaagaga aacggagctc
     4921 ccaaacgttt catcatgtaa aaaccctcaa aaccttcggc tcctcgaaaa cctgaaaaat
     4981 aaacattcaa tggaggtttc atatctccag ggttaaaata gtagataaac tcttgccttt
     5041 tggaatcact gtacctttct ccaccgatta aaaattgacc caaaccttta cgcgaatatc
     5101 gccaatgtag tgcaccaaat ctcaccactc cgtcacctct agcaaagata gaaattgatg
     5161 aaaaaccaac tgattcatca tattctaaaa tatatggatc agccaaatca ttttctgaaa
     5221 ggtattttgt atttaatatc aaatcaagtg atcctttttg gtattgaatg acttctatca
     5281 taatactatt atcaccttgc aatttttgat attcttgcca tatttctatc gctttatcag
     5341 tagaggataa attatagcga aatgagaata gaggttgaaa atcttctcca aaattccctg
     5401 aaaaacaaat agagctattg ccttcatata ttatctctcc cttaaaatca ggatttagat
     5461 ctatgtcaga aaccttcaat tttgcaccat attgttccga aaaaagagta cggcttagga
     5521 aaataatttt ttctttcctt gatccattaa atggtagtgg tctcaaaacc ttacgtttaa
     5581 atataccgtt ttctgagcaa aaatcgagtt gaaaatcctc gtcgtaaaag agtgtatgcg
     5641 cttctaagca atctgaaagt aggttcaatt gcaattcgct aacctcacta gttaaaatga
     5701 ccgcattaaa ccataattta acttcattag ttgaagtttt accaatatat tcaacattat
     5761 ttttttctaa ctctttactt tttaatttct ctaagaaaat ttgaatagca tttccattac
     5821 agtataacca ttcaatattg tctggagtta ttaaatcagc atcccatggt tcctcaccaa
     5881 tctgcaaaat ttttaatttt tccatattat tctttttcta ataattttcg ccagcgctgt
     5941 aatatcttac cgcttgtata atcacccatt ttttttacgc tgtaaactaa cgatttattc
     6001 caattcatta gtccatcaaa ataatagtca attgcattat tgagttctgt agagtcacta
     6061 ataatccagc cattcttctg gtgttctaca taattagtgt gaactttatt gatttgagga
     6121 attccagaac tcagagcagc aatttgagta tataggtctg gatcatctct taaatcaata
     6181 actaacctag cagtatctag agcatctata atttgatttt catttgtata tatatcaaaa
     6241 ctaatgccac gaattttagt tttttgttct tccttaaaat gattctcatc ttccaaagaa
     6301 agagaggtaa aaaaataatc tggattttta gaagacaata ttttgtc
//
LOCUS       DATRSG010000082         6341 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRSG010000082 DATRSG010000000
VERSION     DATRSG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.083"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            247..3864
                     /gene="srr2"
                     /locus_tag="VBD89_001825"
     CDS             247..3864
                     /gene="srr2"
                     /locus_tag="VBD89_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
                     /protein_id="HEO7136328.1"
                     /translation="MLKKQFGNFGEKSRKVRVKMRKSGKHWVKSVMTQIGYVILSRFS
                     GKEKSSKVQTTSEDLSRTKTSASILTAVAALGAVVGGTTDTTSVSAEETPTATELTGN
                     EKTLATAETVVVAPEVKTVNSDSSSHSTSESQSMSTSTLQSTSASLSASESLMDSTSA
                     SLSESSSLSEYSSLSLSSSESVSASESVQSSEAATTARVQPRAMRVVSSASDMETLPA
                     ALISGEGDVTTVQGQDVTDKLQNLDIKLSGGVQAKAGVINMDKSESMHMSLKFTIDSV
                     NRGDTFEIKLSDNIDTNGASNYSIVEPIKSPTGEVYATGIYDSQKKSIVYSFTDFAAS
                     KNNINGILDIPLWPDDTTVQNTKEDVLFSVKIKDQEATIKETVKYDPPVRIDFAGGVS
                     VDSRITNIDDVGKKMTYISQINVDGKSLYNYNGLYTRIYNYSKESTADLKNSTIKIYK
                     TTSDNIVESMVQDYSSMEDVTSKFANSYPEKGWYDIYWGQFIASNETYVIVVETPFTN
                     AVTLNTTLSDYNENNGVEHNHTYSSESGYSDVNAQERKILSELVSSSESVSSSESVSN
                     SESISTSESVSNSESISSSESVSSSESISTSESVSTSESISSSESVSSSESVSSSESI
                     SSSESVSNSESISSSESVSNSESISSSESVSSSESISNSESISSSESVSTSESISSSE
                     SVSNSESISSSESVSSSESISNSESISSSESVSTSESISNSESVSSSESVSTSESISS
                     SESVSNSESISTSESVSTSESISSSESVSSSESISSSESVSNSESISNSESVSSSESV
                     SNSESISSSESVSNSESISTSESVSTSESISSSESVSNSESISSSESVSNSESISSSE
                     SVSNSESISSSESVSNSESISSSESVSSSESVSSSESISTSESVSNSESISSSESVSN
                     SESISSSESVSNSESISSSESVSNSESISSSESVSSSESISSSESVSSSESVSNSESI
                     SSSESVSNSESISSSESVSTSESISSSESVSNSESISSSESVSSSESVSSSESISTSE
                     SVSNSECISSSKSVSNSESISSSESVSNSESISSSESVSNSESISSSESVSSSESISS
                     SESVSNSESILSSESVSSSESISSSESISSSESVSMSTTESLSESEVSGDSEISSSTE
                     SSSQSESMNHTEIKSDSESQHEVKHQVLPETGDNSASALGLLGAGLLLGATKSRKKKK
                     D"
     gene            3974..5227
                     /gene="secY2"
                     /locus_tag="VBD89_001826"
     CDS             3974..5227
                     /gene="secY2"
                     /locus_tag="VBD89_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO7136329.1"
                     /translation="MIGKLYYSYRKSTLLRSILWTILIVGVYMLGQRVLLSTVPLSHQ
                     EIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWMSSMIIWRFVSLFSWAKNATKRK
                     AEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSDIVFKALTMVLLISGSFVLMWLA
                     NLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHFSIMDLLGTIIAISIILSVLLLI
                     STVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAGSYPFMYGMTLMMLPTFIIQALL
                     LAFPNERLLIQISSMIAINKPLGIGLFVILLFILSIGFAYFNYDPYNIAKNMRQRGEY
                     FQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLPMLAVVGKTNQVSIAMIVINIYI
                     ISTFMLSIIEQVNTLKLWGSHRNII"
     gene            5237..>6341
                     /gene="asp1"
                     /locus_tag="VBD89_001827"
     CDS             5237..>6341
                     /gene="asp1"
                     /locus_tag="VBD89_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO7136330.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWPKGT
                     YFVYTPFLVLAKHGGEIIARIQFAENGNLLLIELLIDGVLDKIMIFDDRGFVSSIKYF
                     KQNRLIYQDYLNQNGVWQIREYASKFPRIVVNPMSDKVFNFTEYDDWDTLIRERILVL
                     KKLEVTPDDYIIVAVNDIHNNLVRKSFPDQKKIFSFFGERYSFDNRLDFNELLDEGEL
                     FIAESEKIERLLITSLQEKNYSSNYVENHISRIPPFDTRLRLGQSQNIEELIAYLLVD
                     DLSQTYLSEVLDQLLAKMEVNPSLELKIVSYKND"
BASE COUNT         2087 a         1023 c         1164 g         2067 t
ORIGIN      
        1 acaccttatt tagattagaa agaagcaata gttgatttaa ttttcagaag agaggaagaa
       61 ttgtttattg tagcttttta agataggagg ggtctttata ggcaaatatg ctatttattg
      121 atgatgtttc aagcaatatt ttagaacttg aaataacaaa gtagtatttt gttatagcta
      181 tatttgagaa ttcttagaat tagaaaagtt ttttatatag cattattttt taaatatgga
      241 ggaaaaatgt taaaaaagca gtttggaaac tttggtgaaa aatcacgcaa agttcgagtt
      301 aaaatgagga aatcaggtaa gcactgggtc aaaagtgtta tgacacaaat tggctatgtt
      361 atcctttcga ggtttagcgg taaagagaaa agctctaagg ttcaaactac tagtgaagat
      421 ttaagcagaa ctaaaacgtc tgctagcatc ctaacagcag tcgcagctct tggagctgta
      481 gttggaggga cgacagacac aacttctgtc tcagctgaag aaacacctac tgcaacagaa
      541 cttacaggaa atgaaaaaac attagctact gctgaaactg tagtggttgc gccagaagta
      601 aaaactgtaa actcagattc ttcatcacat tcaacaagtg aatcacaatc aatgtcaact
      661 agtaccttgc aatctaccag cgcctcatta tctgccagtg aatcactaat ggattctacc
      721 tctgcttctt tatctgagtc tagctcgtta tcagaatata gttctttatc tctatctagt
      781 agtgagagtg tctcagcatc agaatcagtt caatcatcag aagcggcaac gaccgctaga
      841 gttcaaccga gagcgatgag agttgtatct agtgcttcag atatggaaac tttaccagca
      901 gcattaatct ctggtgaagg agatgtaaca actgttcaag gacaagatgt aacagataag
      961 ttacaaaatt tagatataaa gctctctgga ggagtgcaag caaaagcagg tgttataaat
     1021 atggataagt cagaaagtat gcacatgtct ttaaaattca ctattgattc tgtgaataga
     1081 ggtgatactt ttgaaattaa gttatcagat aatatcgata caaatggagc ttctaactat
     1141 tctattgtag aacctataaa atcacctacg ggtgaggtct atgcgactgg aatttatgat
     1201 tctcaaaaaa aatctatagt ctatagtttt acagattttg cggcttcaaa aaataatatt
     1261 aatggaatat tagatattcc attatggcca gatgatacga cagttcaaaa cacaaaagaa
     1321 gatgttcttt tttcggtaaa aataaaggat caagaggcta caattaaaga aacagtgaaa
     1381 tatgatccac cggtaagaat tgattttgca gggggagtaa gtgtagattc tcgaataacc
     1441 aacattgatg atgtgggaaa aaagatgact tatataagtc aaattaatgt agatggaaaa
     1501 tcactctata attacaacgg gttatatact aggatatata attatagcaa agagagtaca
     1561 gcagatttaa agaattcaac gataaaaatc tataaaacca cctcggataa tattgtagag
     1621 agtatggtac aggattattc aagcatggag gatgtaacta gtaagtttgc aaatagttac
     1681 ccagaaaaag ggtggtatga tatttattgg gggcagttta ttgcatcaaa tgaaacgtat
     1741 gtaattgttg tagaaacacc atttactaat gcagtaactt tgaatactac tttatcagat
     1801 tataatgaga acaatggtgt agaacataat catacttact catctgaatc gggatattca
     1861 gatgtaaatg ctcaagaaag aaaaatttta tccgaattag taagtagctc agaatcagta
     1921 tcaagttcag aatcggtatc taattccgaa tctatttcaa cttctgaatc ggtatctaac
     1981 tccgagtcta tttcaagttc cgaatcggta tcaagctcag aatctatttc aacttctgaa
     2041 tcggtatcaa cttcagagtc tatttcaagc tccgagtcag tatcaagttc agaatcggta
     2101 tcaagctcag aatctatttc aagttccgaa tcggtatcta actccgagtc tatttcaagc
     2161 tccgaatcgg tatctaactc cgaatccatt tcaagttctg aatcggtatc aagctcagaa
     2221 tctatttcga attcagagtc tatttcaagc tccgagtcag tatcaacttc agagtctatt
     2281 tcaagctccg aatcggtatc taactccgaa tccatttcaa gttctgaatc ggtatcaagc
     2341 tcagaatcta tttcgaattc agagtctatt tcaagctccg agtcagtatc aacttcagag
     2401 tctatttcaa actccgagtc ggtatctagc tccgagtcag tatcaacttc agagtctatt
     2461 tcaagttccg aatcggtatc aaactccgaa tccatttcaa cttccgagtc agtatctacc
     2521 tcggaatcta tttcaagttc tgaatcggta tcaagctcag aatctatttc aagttcagaa
     2581 tcggtatcaa actccgagtc tatttcaaac tccgagtcgg tatctagctc cgagtcagta
     2641 tcaaattcag agtctatttc aagttccgaa tcggtatcta actccgaatc catttcaact
     2701 tccgagtcag tatctacctc ggaatctatt tcaagttctg aatcggtatc aaattcagag
     2761 tctatttcaa gttccgaatc ggtatctaac tccgagtcta tttcaagttc agaatcggta
     2821 tcaaattcag agtctatttc aagttccgaa tcggtatcaa attcagagtc tatttcaagt
     2881 tcagagtcag tatcaagttc agagtcagta tcaagctcgg aatctatttc aacttcagaa
     2941 tcggtatcta actccgagtc tatttcaagt tccgaatcgg tatctaattc tgaatctatt
     3001 tcaagttccg aatcggtatc aaattcagag tctatttcaa gttccgaatc ggtatcaaac
     3061 tccgagtcta tttcaagttc cgagtcagta tcaagctcag aatctatttc aagttccgaa
     3121 tcggtatcta gctccgagtc agtatcaaat tcagagtcta tttcaagttc cgaatcggta
     3181 tctaactccg agtctatttc aagttcagaa tcggtatcaa cttcagagtc tatttcaagt
     3241 tccgaatcgg tatcaaattc agagtctatt tcaagttcag agtcagtatc aagttcagag
     3301 tcagtatcaa gctcggaatc tatttcaact tcagaatcgg tatctaactc cgagtgtatt
     3361 tcaagttcca aatcggtatc taattctgaa tctatttcaa gttccgaatc ggtatcaaat
     3421 tcagagtcta tttcaagttc cgaatcggta tcaaactccg agtctatttc aagttccgag
     3481 tcagtatcaa gctcagaatc tatttcaagt tcagaatcgg tatcaaactc cgagtctatt
     3541 ttaagttccg aatcagtatc aagctcagaa tctatttcaa gttcagaatc tatttcaagt
     3601 tcagagtcag tatcaatgag tactacagag tctctaagtg aatcagaagt atcaggggat
     3661 tctgaaatta gctcaagtac agaatcatca agtcaatctg aatcgatgaa tcatactgaa
     3721 attaaatccg attccgaatc tcaacacgaa gttaagcatc aagtattacc agaaacaggt
     3781 gataactcgg cttcagcatt aggtctgtta ggtgcaggat tgttgttagg agctactaaa
     3841 tctcgcaaga agaaaaaaga ttaattttca aagtgatatt tagatgataa atcgaaagta
     3901 ctttcgattt atcacttaag atagtaagta gttatactgt cttaagtgat aaattgattt
     3961 gaaaagggaa aaaatgattg gaaaattata ttatagctat agaaagtcaa cgctattaag
     4021 aagtatttta tggactattt taattgttgg tgtatatatg ttaggacaac gtgttttatt
     4081 atccactgtt cctttatcac atcaagagat aaaactagca gtagatcaac atttactcaa
     4141 taacttttca gcagtaagtg gtgggagttt taataaatta aatgttttca cactggggtt
     4201 gagtccatgg atgtcaagta tgattatttg gagattcgtt tccttatttt cgtgggcaaa
     4261 aaatgcaacg aagcgaaaag cagaagtagc tcaatatact ttaatgctta ctatctcagt
     4321 tatacaagca tatggtgttt caggaaatca atttataaaa agctctttat taggttctta
     4381 tagtgatatt gtttttaaag cattgacaat ggtcttgtta ataagtggct catttgtgtt
     4441 aatgtggctt gctaatctca atgcttctaa agggctagga ggaagtattg ttatcatatt
     4501 agtgagcatg ctccaagcaa cgactactgg aataataact tattttgaac agtcacattt
     4561 tagtattatg gatctgctgg gaactattat agctataagt ataatactta gtgttttatt
     4621 attaatctca acggtggttt atagggcaga atatcgtata cctattaggc gtattgatat
     4681 cgttagtaaa tttgcccaag atacctatat tcctattcgc ataaatccag cagggtccta
     4741 ccctttcatg tatgggatga cattaatgat gcttccaacg tttataattc aagcattgtt
     4801 acttgctttt ccaaacgagc gattattgat tcagatctcg tcaatgattg caattaataa
     4861 gcctttaggt attggacttt ttgttatatt attatttatt ttgagcattg gatttgcata
     4921 ttttaattac gacccgtata atattgcaaa aaatatgcgt caacgggggg aatattttca
     4981 aaatgtaact ccaggagctg ctactcaacg ttttttacaa gaaaaaatta gtatcatggc
     5041 agtagttgga gcaattttta cggttattat gggaggtctc ccaatgttgg ctgtggtggg
     5101 gaagacaaac caagttagta ttgcaatgat cgtcattaat atttatatta tttcaacttt
     5161 tatgctcagt attattgagc aagttaatac cttgaagttg tggggcagtc accgaaatat
     5221 tatttaagga gcaatcatgt tttattttat tccttcgtgg tacaattctg atcaaaagtg
     5281 gcataatgaa gctcctatct ggtatcgtgt attggagaac atgacttttg atgatactgt
     5341 tagtcaggtt agaatgtttc agaatgcaga tgaggaagtg cgtttacttc tatttagtta
     5401 taatcctcag ttgagataca tgcttcatga agagagtatg ttggctgtct cttattggtc
     5461 tttttttgac gatattcaaa atattcaaca aaaagagatt aagttaatta attttaaaga
     5521 actgaattgg cctaaaggga cttattttgt ttatacacct tttttagtat tagctaaaca
     5581 tggtggcgag attattgcac gtatccaatt tgcggaaaat gggaatttac tactgattga
     5641 acttttaata gatggtgtgc ttgataagat tatgatattt gacgatcgtg gttttgtatc
     5701 cagtatcaaa tatttcaaac aaaatagatt aatttatcaa gattatttaa atcaaaatgg
     5761 tgtttggcaa attcgagagt atgcttcaaa atttccgaga atagtagtta atccaatgtc
     5821 tgataaggtg tttaatttta ctgagtatga tgattgggat actcttattc gagagagaat
     5881 attggtttta aaaaaactag aagtgactcc agatgattat attatagtag cagtaaacga
     5941 tattcataac aatttagtca gaaaatcatt tccagatcaa aagaaaattt tctcattttt
     6001 tggtgaacgc tattcatttg ataatcgact cgactttaat gaacttcttg atgaaggtga
     6061 attgtttatt gctgaaagcg aaaaaataga acggttattg attacttcat tacaagagaa
     6121 aaattattcg tcaaactatg ttgagaatca cattagccgt attccccctt ttgatactcg
     6181 tttgagactc ggacagagcc aaaatattga agagcttatt gcatatttac ttgtagatga
     6241 tctttctcaa acttatttaa gtgaagtttt agaccaatta ttagcaaaaa tggaagttaa
     6301 tccgtcatta gagttgaaaa tagtcagcta taaaaatgat t
//
LOCUS       DATRSG010000083         6077 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRSG010000083 DATRSG010000000
VERSION     DATRSG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.084"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(19..1800)
                     /gene="uvrC"
                     /locus_tag="VBD89_001828"
     CDS             complement(19..1800)
                     /gene="uvrC"
                     /locus_tag="VBD89_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO7136331.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGNPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     DIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            1959..2726
                     /locus_tag="VBD89_001829"
     CDS             1959..2726
                     /locus_tag="VBD89_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO7136332.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(2785..4125)
                     /locus_tag="VBD89_001830"
     CDS             complement(2785..4125)
                     /locus_tag="VBD89_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7136333.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(4225..4641)
                     /locus_tag="VBD89_001831"
     CDS             complement(4225..4641)
                     /locus_tag="VBD89_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7136334.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(4645..5142)
                     /locus_tag="VBD89_001832"
     CDS             complement(4645..5142)
                     /locus_tag="VBD89_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7136335.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            5364..5960
                     /locus_tag="VBD89_001833"
     CDS             5364..5960
                     /locus_tag="VBD89_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO7136336.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
BASE COUNT         2098 a         1150 c          903 g         1926 t
ORIGIN      
        1 actgttctac taagtttttt atttaggagg atcattaaga gaatctcgga gactttctgc
       61 aactgtccta ggaataccac acataataat gtcctcaata tcagcttttt ggatattagg
      121 taagctttta aaatgtttca ttagcaattg tttacgtttt ggtcccaacc cagtgatacc
      181 atctaatttt gaagaaaagg aatttttgga gcgtaattga cgatgaaaag taatagcaaa
      241 acgatgtacc tcatcttgaa tacggtgaag taagaaaaat tcttctgaat tacgcggtaa
      301 ttctactact tctaaaggat ctccaaaaag taattcatgt gtttggtgtt tatcattctt
      361 ttgcaaacct gcaataggta tggctaaacc taattggttc tcaataacat cacgcgctat
      421 atttacttgc ccctgaccac catcaattac aatcaagtcc ggaggtgtca agccatcttt
      481 taaaacccta ctataacgcc gatgaatgac ttctctcata ctagcataat catctggtcc
      541 gataacagtc ttaattttaa actttcgata gtctttttta cttggatttc cattcacaaa
      601 taccaccata gcagctactg gacttgtccc ttgaatattg gaattatcaa atgcttctat
      661 gcgtactggt ttagggatat taagaagatt acctaaattt tcaattgccc catgagtttt
      721 acgaatatct ttttccagta agtcaaactt ctgttgtaaa ctgacacgag cattttttat
      781 tgccagattt actaactgtt tcttttcacc acgttgtggt ttaacaatct tacagcctac
      841 aattgtttca accgacttag catcaatatc ctgtggaata aagacttctt tgggtagaaa
      901 atgttttgta tcttgataaa attgccctat gtaagttaga aaatcttcct ctggttcatt
      961 ataataagga aacatattca cgtcacgctg aatcaatttt ccatttcgaa caaaaaagac
     1021 ctgtacacac atccaaccct tatcaacgaa ataaccaaaa acatctctat ccttcatatc
     1081 ttgatgaata acacgctgct tagtacgcaa caaactaatt gcttcgatca aatctcgata
     1141 ttcagccgca cgctcaaatt ccatagtcat agcagcgctc ttcatttttt cagttaatcc
     1201 gttaacaatt ttattatctt taccattaag aaactgtttt acatcttctc tcagactatc
     1261 ccaataagct ttattagttt gacaaactgt atgtgcattg cattgcccta aatgataata
     1321 aaagcatact ttatttgctg gattagtaca tttcttaaaa ggaaagagcc tatctagtaa
     1381 ccgcttaatc tcagttgctg ccccagaatc cggataaggt ccaaaataag tgccatctga
     1441 tttttttact tgacgtgtaa tcataagacg aggataacgt tcatttgtta ttttgatata
     1501 tgggtaagac ttatcatctt tcaatcgaat attatacttc ggcatatttt cttggattaa
     1561 attaatttcc aataatagag cttcggtatt agatgttgtc acaatgtatt caaagtcttc
     1621 aatctcagag accaaaagtt ctgtcttagt attatgactc ccatgaaaat aacttttcac
     1681 acgattctta agatttttag ccttacctac ataaataatt gtcccattct tatctttatg
     1741 aaggtaacaa ccaggactgt ctggtaacaa ttctagctta tgtttaatca gttcattcat
     1801 agaactatta taacaaaatg tactgtaatc tttacgaaat aaatactatt ccccaacatc
     1861 tattgtatct tccaatactt gaataaaacc ccaatactcc tagtctaagc aattatttta
     1921 tgttacactt taaacaagtg aattattgag gagtttttat gatagacgtt gagaatattc
     1981 ttgaaaaaat gaaacctaac caaaaaatca attacgattg ggtcatgcaa caaatggtta
     2041 agcagtggca agctagtgat atccgtccga aaattctgat gcatgtttgc tgtgctcctt
     2101 gtagtacata tacccttgaa tacttatcac aatgggcaga tgttaccatc tattttgcta
     2161 attcaaatat ccatcctaaa gatgaatatt atcgccgcga atatgttact caaaaatttg
     2221 ttcatgattt caacaaaaat actggatatt ctgtacaatt cttatcagca ccatatgaac
     2281 caaatgaatt tttcaaaatt gttcatgggt tagaagaaga acctgaaggt ggggatcgtt
     2341 gtaaagtttg ctatgatttc cgtcttgata agacagcgga aaaagccgtt gaactaggtt
     2401 ttgattactt tggcagtgcc ctaactatta gtcctcacaa aaactctcaa acaatcaata
     2461 ctattggtat tgatgtccaa aaaatttatg atactcagta tcttcctagc gaccttaaaa
     2521 aaaataaagg ttaccaacgt tccgttgaga tgtgtaaaga ttatgatatc taccgacaat
     2581 gttactgtgg gtgtatcttc ggtgctaaag atcaagggat aaatcttctc caaatcaaaa
     2641 aagatgcgaa agctttcgtg agtgataaag acggcaaaga agaatttcct aatatacgct
     2701 tcacatttaa tggcaagtca atgtaaatga taaaaaaaga cttagtaaag aactaagcct
     2761 ttttggtata aatcttgtca aactttaata atacaacttc ttaatctttt gcagacgttg
     2821 gtttagaaat agaccacaaa caaaaatacc tgtgattaaa ccaatccaat aggcaaacgg
     2881 acctaactgg ctattttttt ctaagataac cgctaatggc aaagcacata accaataaga
     2941 gcccgcaccg atcataaatg gttttgttgt atccttatag cctcgtaaaa tcccctgtac
     3001 aggagctgca taagcatctg caaactggaa aaagagacta taagttagga attgagctgt
     3061 aatagcgaca aagtgagggt cactattata cattgctgct acattctcac gaaatagaaa
     3121 taaaaatagt aaggttcctg atgtaatccc taccgctgtt aagcgtccta tcctactata
     3181 agtgtttgcg tcctgaaagc gctctgcccc tacttcaaac gatattgtaa tagctagagc
     3241 agtggaaata cttaaaggaa atgcatacat taatgatgaa aaattcatag cagcctgatg
     3301 tgctgcaata atgattgaag aaaattttgc catgaataag cctactactg caaaaattgc
     3361 aacttctgca aaaatttgta aaccaatcgg taatcccaat cgaatatctt caataatcaa
     3421 aggagctttt attctttcca gagtccatat atgatatgtt ttaatttgag gatgaagtga
     3481 catcacaata ataataacaa taaaaatagc ccaataagtt aaagaagttc caagacctgc
     3541 ccccgcacct cctagtctag gcataccaaa tttaccgtag ataagcatat aattaaaaaa
     3601 tgaattaaag ggtagaatta aaagcatcag atacatagat aaccttgtta accccaatgc
     3661 atcaaagaat gaacggcaaa tgctaaacaa caccagcggc atgattccaa tcaacatata
     3721 atttaaataa ccacgaccaa ctgctagaac ttcatcttct aaacccaaac tccccaagac
     3781 aggttgagca ataaattgca tgattaaaaa taatattaag gacagtatca aacctaaata
     3841 tagaaattga tgaaattctg tgcgaatttg ttctttattt cctctaccca aatgttgacc
     3901 aactactggt actaatgctg aaatcatacc tactaataaa gcgaaaaacg gagtccataa
     3961 attactagca gttgacacac ctgccaaatg tagctgacta tactgtccag tcataaccga
     4021 atcaataaaa gtagctgaaa aattagcaaa ttgataaatc aatactggga taaataattt
     4081 caaaaataaa acaaattttt ccttatttgt ctgagtttga tacatctttt ttctcctttt
     4141 caccttaatc aatttatttt agcttagcta ggatactctg tcaatgctat ttaaaaaggt
     4201 tgagaaataa tcctcaaccc cattttagct aagctcagta ttgatttgcc atgtcatacc
     4261 atatttgtca gtcacatttc cataagctgg actccacgga actgcttgta gaggcatttt
     4321 aatgttcatt gcatctcgag aaagcttatt gaataactct tgcgcttctt ctacagattc
     4381 cacaattaaa gctgctgtca tattttgtcc aatttgaaaa tcaaaaatag gattattgtc
     4441 agaaagttgc attcgaatac catcaatatt aaattgagcg ttcataatta aatgttcaag
     4501 ttctggcgga caatctggcc aaccctgtgc aaaagttgtg acgtgttctg cttttgtatt
     4561 aaaagcctct tcataaaatg taatagcttc ggccccacga ccattaatat tcatataaat
     4621 ttcaattgct ttcattacca tacctcactt catattatcc aaccaacgct gcatcagagt
     4681 ttcattttcc ccttttaggg taaaataaga accacgtatt atacctgcga gttttgactt
     4741 ttgacgagca aacttaaact tgtccgctaa ttcaatagga atatccattc catctgataa
     4801 ttttaaacct acagctctct ttttaggatt atctaattca tataaaatat taatggctaa
     4861 accatcctca tagaaaacat gtgtccataa aatacgattc tctaaaaaaa gctgtgaaat
     4921 ctctaaaggc tttgcagcaa aaacaaggtt acgttcattt tttagaaggt gattaatatc
     4981 tgccatcaat tctggatagt ctgatactgg aaatgtttcg taggctactt tgaatttatt
     5041 ccagaaatag cgagcttcat ttgcacgcaa accagccaag gcttctgcca ctggagaagt
     5101 ctccaaacct ttagttgaaa cggttttaaa gtcgataatc atgttctttc ctttttgatt
     5161 aaaatcttcg ataatttagt atactctttt ttgaagaaaa agtacattct aatcacttat
     5221 aaaaagtagt acatttagaa cactaccacc tcagtaattt cttgaccttt ctctacttaa
     5281 tacttataat taatctagac ttaatgtcaa agcatcaatc aaggtgtaaa cctgattcac
     5341 ctaaaaagct gatggagcta aacatgacca aaaagatatt cttatttatc tgcgctactc
     5401 ttttagtagg aatagcctta tatacaccaa caacttctat ttttaatcat aacaacgttt
     5461 atgctgccac caagaaagca aaaattaaat ttaataaaac tcaaaaaaaa attgtcaaaa
     5521 aagcaagaga atatgctaag tctggccata tgtctaaaga tagtatcatt gaaaaattaa
     5581 aaaaggattc taagaaatat cgtcaagaag acatcaactt tgtgattaat aacctcaagg
     5641 ttgattacaa aaaaaatgcc ttgatttctg ccaaaattta ttcaaagacc atgaatttat
     5701 ctaaacaatc tatctttgaa cagctctatt cggagtctcc agataaggca acccactctg
     5761 ataaatttac aaaagaagaa tcccaatatg ctatagacca tctcaaagta gatttcaaag
     5821 aaaatgctct tgagactgct aaaagttatc aaagtagttc ttctttatcc aaagaagaaa
     5881 tctataaaca attgacatct acgctgggcg ataaattcac aaatgatgaa gcccaatatg
     5941 ctgtagatca tttgaaatga tgacatacca atagtctatc cttatgtaat agaattgata
     6001 gcttatgcta atattccagc tgtaaaatag caagaaccct cctaagttca ttaagactta
     6061 ggagggtttt tatcaat
//
LOCUS       DATRSG010000084         5810 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRSG010000084 DATRSG010000000
VERSION     DATRSG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.085"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1329)
                     /locus_tag="VBD89_001834"
     CDS             complement(<1..1329)
                     /locus_tag="VBD89_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEO7136337.1"
                     /translation="MNKLVIKSLLIVDYENKKANKFRFSDSANLIVSSTNGEGKSSLV
                     KSIYYALGANLKSFPKGWNNSDYIFQLEVSIDDKDFLIKRHNKVISISDNGRVQVFEN
                     FKEYSSWFQKKLGMNLELVSKNSDTPSLASVEALLSPMYIDQDKSWDGKLFKDSFEGL
                     GRYKPSDFPKNVIDYYLNISDSKILDKESEKNEYSKQLDLVNGKITQIQSVYETYRSQ
                     KNVTETVPKNFEELKKELNFYIRETDKFSKEITEKIGELSKERIKLDILQQDLEELKK
                     LSSKTKKRFSNIKHECVYCHSILTREQSLTRLELADNELAINVRMDEISQQIDKSERK
                     VQEKEGAIERLEEQFYQYHERLAELKQLSNIEEYVNQNVLSELKKLEIQENLEKTKLD
                     SNISELKKEVIKLKKELNQRAKEIEGEYEELKNELSVQIGSAGITDKKFRN"
     gene            complement(1322..2257)
                     /locus_tag="VBD89_001835"
     CDS             complement(1322..2257)
                     /locus_tag="VBD89_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HEO7136338.1"
                     /translation="MAKDNGGAIAQKGFNYQNCVVSLVAIRNYKKPNFSIYVEADEDF
                     EVMYDDDYHAYIQVKGQKGMSIKKLLSTTNNKPSIFEKNLSSGTDDSKYKIVVYNFNE
                     KDLKEMQEQIDTEELFYSSWLLSDSQKNKVSNPKTKNFSLVKTDFDNNISAARTFLKG
                     ELANQKISIDNRDDVILDELLQQIIQKSEKEIHSDADKELKKITSEELNLILQKITAK
                     ARFDKELKKFGFTSIKNEKIKREEKKIILEYMTAKKAVISFFKSDEDRLENEEMTILI
                     PKSFDLSEMHTLSENSKYAIGISAYCDILEGIANE"
     gene            complement(2274..3494)
                     /locus_tag="VBD89_001836"
     CDS             complement(2274..3494)
                     /locus_tag="VBD89_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEO7136339.1"
                     /translation="MQFIPYDDELEVINNLHKYNSNDYTVIRLTQTMLNKSIIDANGF
                     LRTLLLDNDLLDFRNLTDKVYLTANLVLDNQKHRVKISFYKANKRGDERFWIYGLGKF
                     IKSSQINVNDLIYITVNNQKELTLFNVTHSIPQNSTIIELFGQDKVEDSLNRLIPQIK
                     AIARQGFHNNSKGAGKIAPKDAGDTLESLLGIQTNNSQEADFEGIIELKSKSSKTLDT
                     LFTLRPNFEGTPVAEFEPNDRSRVSAFARLYGYDSDKHLGYKSLYITIGSEENPQNNQ
                     GFYLEVNDSDNIVELRKQENKKSILTAFWTFEALEEELHKKHPATLWVKVESKMDGEV
                     GQFKYVEAELTRSPQFMTFLSLIKSGAITYDWRGYTTPAGKYVGKNHGNAWRIKNKQR
                     GLLFGSTEVIDLSF"
     gene            complement(3494..3955)
                     /locus_tag="VBD89_001837"
     CDS             complement(3494..3955)
                     /locus_tag="VBD89_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7136340.1"
                     /translation="MERYVKENIERLLYTSGLTQNELADKANLSRQTVNKLLSSNEKY
                     SPKLSTLIAISNVLLINFPELLIRSNKLSASINFTLNSVEEYQNTLIQNVKLYLRSWK
                     QFSLSNSSGVRESTISNLVNGKYSDVYFSTIESLAKEMEVPLADLFKRGES"
     gene            4149..5699
                     /locus_tag="VBD89_001838"
     CDS             4149..5699
                     /locus_tag="VBD89_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEO7136341.1"
                     /translation="MPYAIDLFCGAGGFSEGILQAGFDIIFSSDKSPMVEETYTNRHK
                     QLGLVEGVDTHFELADIKELTSDKIFQSINSLKYGNIFERGTIDVIFGGPPCQGFSRL
                     GKRDSKDPRNMLFHEYLRIIKDVMPRYVVMENVTGILDMQMLDFPSVLNDRIYGGQHL
                     VQSILRNELEELGYTLLDVQVLNAANFGVPQQRNRVVFLAYRNDVAPIQYPESDNDIP
                     NATVYDALGDLYGGEFSYETNYSLQSRLGRTPNTDGHPITNDNPLNMEISRHDDIVTQ
                     RFSLYQQGENRAKAVNRIQLEGLELKRDYPNLFIESLFQINGKANQLHIIKQLKKQDL
                     LQESFKSERWLQFTNRQLGLLSMNDTDNSAKQHILKSLALRLKTSFEEAELFWNEIKN
                     QLNSDITEDKFSKMLRDGDLTPEAIEAIFTKKSIRVRLDQNSVSPTMVTLPDDYIHPF
                     FNRVLTVREMARLQSFDDSFEFLGKRTTGGDKRAKETPQFTQVGNAVPPLLAKAIAEQ
                     VMIALNND"
BASE COUNT         1800 a         1009 c          847 g         2154 t
ORIGIN      
        1 attacgaaat tttttatcag taattcctgc agaaccgatt tgaacagaga gctcattttt
       61 aagttcttca tactctcctt caatttcttt agccctctgg ttaagttctt tttttagttt
      121 gatgacttct tttttgagtt cactaatatt tgaatcaagt tttgtttttt ctagattttc
      181 ttgaatttct aactttttca gttccgataa aacattttga tttacatatt cttcaatatt
      241 agatagttgt tttaactctg ctaaacgttc atgatactga taaaattgtt cttccagtcg
      301 ttcgattgcc ccttcttttt cctgaacttt tctttcagat ttgtcaattt gttgggaaat
      361 ttcatccatt ctaacgttga tagcaagttc attatctgct aactccaatc gcgttaagga
      421 ttgttccctt gttaaaatcg aatgacaata aacgcattcg tgtttaatat tagaaaatct
      481 tttcttagtc ttacttgata atttcttgag ttcctctaaa tcttgttgta agatatcaag
      541 ttttattctt tctttggata gttcacctat tttttcagta atctcttttg aaaacttatc
      601 tgtttcccga atataaaagt tcaattcttt tttcagttct tcaaaatttt tagggactgt
      661 ttctgtaaca ttcttttgag aacgatatgt ttcatataca gattgaattt gagtaatttt
      721 tccattgact aaatccaact gttttgaata ctcattcttt tcactttctt tgtcaagaat
      781 cttactatca gaaatgttta aataataatc aataacattt tttggaaagt cacttggttt
      841 atatcgtcct aaaccttcaa atgagtcttt aaataatttc ccgtcccatg atttatcttg
      901 gtcaatatac attgggctca gaagagcctc aacagatgct aaactagggg tgtcgctatt
      961 tttactaacc aactccagat tcatgcccaa ctttttttga aaccatgaag aatattcttt
     1021 gaaattttca aacacctgaa ctcttccatt gtctgaaatt gaaataactt tattgtgtct
     1081 ttttatcaaa aaatccttat catcaataga gacttccagc tgaaagatat aatcgctatt
     1141 gttccaacct tttgggaaag acttaagatt ggctcccaaa gcataataaa ttgatttaac
     1201 tagactagac tttccctcgc cgtttgtact actgactatt agatttgctg aatcagaaaa
     1261 tctaaattta ttagcttttt tgttttcata gtctactatc aaaagacttt ttattactag
     1321 tttattcatt agcaatcccc tccaaaatat cgcagtacgc tgatatccca atagcatact
     1381 tactattctc cgataatgta tgcatttcag ataaatcaaa cgatttgggt atcaaaatag
     1441 tcatttcttc gttttctaat ctgtcttcat cgcttttaaa aaaactaata acagcttttt
     1501 tagcagtcat atattctaag ataatctttt tttcttctcg tttaatcttt tcgtttttta
     1561 tagatgtaaa tccaaatttt tttaactctt tatcaaacct tgcttttgca gttatttttt
     1621 gtaaaattag atttaattct tctgatgtaa tctttttaag ttctttatca gcatctgagt
     1681 gtatttcttt ttctgatttt tggataattt gctgtaataa ttcatcaagg attacatcat
     1741 ctctattatc aatagaaatt ttctgattcg ctagttcccc ttttaaaaat gtcctagcag
     1801 cgctgatatt gttatcaaaa tcagttttca ctaacgaaaa gttttttgtt ttaggattgc
     1861 taactttatt tttctgacta tcagataata gccatgagct atagaaaagt tcctctgtat
     1921 ctatttgctc ctgcatttcc ttcaagtctt tttcattaaa attatacacc acaatcttat
     1981 acttagagtc atctgtacca gacgatagat ttttttcaaa gattgacggt ttattattag
     2041 tggtactcaa taatttttta atactcatgc ctttttgtcc tttaacctga atataggcat
     2101 gataatcatc atcgtacata acttcaaaat cttcgtctgc ctctacatat attgaaaaat
     2161 taggtttttt gtaatttcta attgcaacta aggacacaac acaattttga taattgaatc
     2221 ccttttgtgc aattgcccca ccattatctt ttgccataaa atttctccaa atactaaaaa
     2281 cttaaatcaa ttacttctgt actaccaaat aacaaacctc tttgcttatt ttttatccgc
     2341 caagcgttac cgtgattctt acctacatac tttcctgctg gtgtagtata gcctcgccaa
     2401 tcataagtaa ttgctcctga ttttatcaga gaaagaaaag tcataaattg tggtgaacga
     2461 gttaattctg cttcaacgta cttgaattga cctacttcgc cgtccatttt agattcaact
     2521 ttcacccata aggtggctgg gtgtttttta tgtaattctt cctctagagc ttcaaatgtc
     2581 caaaaggcag ttaaaatact ctttttgttt tcctgtttcc gtagctcaac aatattatcg
     2641 ctatcattaa cttccagata gaaaccttga ttgttttgag gattttcttc tgaacctatt
     2701 gtaatgtata agctcttata tcccaaatgc ttatctgagt catagccata cagacgagca
     2761 aacgccgaca ctctactcct atcattcggt tcaaattctg ctacgggcgt tccttcgaaa
     2821 tttggacgta aagtaaatag ggtatctagg gttttagagg attttgattt tagctcaatg
     2881 attccttcaa aatcagcttc ttggctatta ttggtttgaa ttcccaaaag tgattctagg
     2941 gtatctccag cgtctttcgg tgcaatctta cctgccccct tagaattatt atgaaagcct
     3001 tgtcttgcta tggctttaat ctgtggaatt aatctattta gtgaatcttc aactttatct
     3061 tgtccaaata gttctataat agtactattt tgtggaatgc tatgtgtaac attaaataat
     3121 gtaagttctt tttgattatt tacggttata taaatcaagt cgtttacatt gatttgagat
     3181 gacttaatga acttccctaa tccatatatc caaaatcttt catctcctct tttgttagct
     3241 ttatagaaac ttatttttac cctatgcttt tggttgtcca gtacaagatt agctgtcaaa
     3301 taaactttat cagtcaagtt cctaaaatct aacaagtcat tatctaatag caaagttctt
     3361 agaaatccat ttgcatcaat aatactttta tttagcatag tttgtgtcaa tctgataaca
     3421 gtgtagtcat tagaattata cttatgtaga ttattgataa cctctaactc atcatcatat
     3481 ggaataaatt gcattagctt tctcccctct taaataaatc agcaagcgga acttccattt
     3541 ccttagcaag gctttctatt gttgaaaaat atacgtctga gtatttacca tttacaagat
     3601 tacttatcgt agattctctt actccactgg aattacttag tgaaaattgt ttccaacttc
     3661 tcaaatataa tttcacattc tgtataagtg tattttgata ttcttctaca gaatttagag
     3721 taaagtttat agaagcactt aatttattgc ttctaataag gagctctgga aaatttatta
     3781 ataagacatt agaaattgct attaaagttg aaagtttagg actatacttt tcattagaag
     3841 ataatagttt attaacagtt tgcctgctaa gattcgcctt atcagctaac tcattttgcg
     3901 ttaatccgct tgtgtaaagt aaccgttcaa tattttcttt aacatagcgt tccatatcta
     3961 ttttctccaa ctattgtttt tatttatatt aaagctttta attataataa aattcatttt
     4021 actttacatt ttgactggtt ttagaaaaaa tttactcatg tataaagtag aaaagtagta
     4081 acacctggac ttttcaatga aattctgata tactactaaa gtaataaata cataattcga
     4141 ggtaaattat gccctatgca atagacttat tctgtggtgc tggcggattc agtgaaggaa
     4201 ttctacaagc tggatttgat attatcttct ctagcgataa aagcccaatg gtagaagaaa
     4261 cttatacaaa ccgacataaa caactaggtt tagtagaagg tgttgataca cactttgaac
     4321 tcgcagatat aaaagaatta acttctgata aaatttttca atctattaac tcattaaaat
     4381 acggaaatat ttttgaaaga gggactattg atgtcatttt tggtggtcct ccttgtcaag
     4441 gattcagtcg tttaggaaaa agagattcca aagaccctag aaatatgctc tttcatgaat
     4501 atttgcgtat tataaaagat gttatgccac gttatgttgt tatggaaaat gtaactggta
     4561 ttttagatat gcagatgtta gacttcccta gcgtactcaa tgatagaatc tacgggggac
     4621 aacatttggt tcagtccatt cttagaaatg agctggaaga attaggatat actttacttg
     4681 atgtgcaggt tcttaatgct gcaaactttg gtgtcccaca acaaagaaat cgtgttgtat
     4741 ttctagcata ccgtaatgac gtagcaccca ttcaatatcc agaaagtgat aatgatattc
     4801 caaacgctac tgtctatgac gctttaggag acctttatgg tggggaattt agctatgaaa
     4861 caaattattc cctacaaagt cgactgggta ggactcctaa tactgatggt caccccatta
     4921 caaatgataa tccgttgaat atggaaatct caagacacga tgatattgtc acacagcgat
     4981 tctcactata tcaacaagga gaaaatagag ctaaagccgt taatagaatc caattagaag
     5041 gactagaatt aaaaagggac tatccaaacc tatttattga aagtctattt caaatcaatg
     5101 gtaaagctaa ccagcttcac attatcaagc aactaaaaaa acaggatttg cttcaagaaa
     5161 gttttaaatc cgagagatgg ttacaattta ccaatcgtca gcttgggtta ttatcaatga
     5221 acgatacgga taactctgct aaacaacata ttttaaaatc attagcgctt agattaaaaa
     5281 cttcatttga ggaggctgaa ctattttgga atgaaatcaa gaaccagttg aattcagata
     5341 ttacggaaga taagttttca aaaatgctaa gagatggaga tttaactcct gaagcaatcg
     5401 aagccatctt tactaaaaag agtattcgag ttcgtcttga ccaaaatagt gtctcaccaa
     5461 ctatggtaac attaccagat gactatatcc acccattctt taaccgtgtt ttaactgtta
     5521 gggaaatggc tcggctgcaa tcttttgatg atagttttga atttcttggg aagcgtacca
     5581 ctggtggaga taaacgggca aaagagacac cacaatttac tcaagtaggt aatgctgtcc
     5641 caccattact ggctaaggca attgctgaac aggtgatgat tgctctaaac aatgattaaa
     5701 aagcttttta tatactccaa atatttcatt ttgaattatg tctgtaacat ttttctagta
     5761 taatactata aaaggaggtt tggaaaatga aaaaaatact tagtctatta
//
LOCUS       DATRSG010000085         5724 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRSG010000085 DATRSG010000000
VERSION     DATRSG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.086"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            120..1265
                     /locus_tag="VBD89_001839"
     CDS             120..1265
                     /locus_tag="VBD89_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136342.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITTEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1265..1900
                     /locus_tag="VBD89_001840"
     CDS             1265..1900
                     /locus_tag="VBD89_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136343.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1903..2829
                     /locus_tag="VBD89_001841"
     CDS             1903..2829
                     /locus_tag="VBD89_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7136344.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2830..3480
                     /locus_tag="VBD89_001842"
     CDS             2830..3480
                     /locus_tag="VBD89_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136345.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3542..4723)
                     /locus_tag="VBD89_001843"
     CDS             complement(3542..4723)
                     /locus_tag="VBD89_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7136346.1"
                     /translation="MNINGIKLLSSRAVSKLGDVLYDYGNSTWIASIGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWISYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4796..5649)
                     /locus_tag="VBD89_001844"
                     /pseudo
     CDS             complement(4796..5649)
                     /locus_tag="VBD89_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
BASE COUNT         1936 a         1024 c          929 g         1835 t
ORIGIN      
        1 gtagatcggg cgcaagctta acgattcttt ttaaaatcat taaattttaa aacaaatttc
       61 agacatattg ccaaagtttt gatattatta ctataatata gtttgtagag gagaataata
      121 tgggccaaga acctatcatc gaatatcaaa atatcaataa agtgtatggg gaaaatgttg
      181 cggttgaaga tattaacctt aaaatttacc ctggtgattt cgtttgtttc atcggtacga
      241 gtggatcagg taaaacaaca ttaatgcgta tggttaacca tatgttaaaa ccaacaaatg
      301 gtactctatt atttaaggga aaagatatct ctactattaa ccccattgaa ttaagacgca
      361 gaattggata tgttatccaa aacattggtt taatgcctca tatgaccatt tacgaaaata
      421 tagttcttgt accaaaatta ttgaaatggt cagaagaagc taaaagagct aaagcaaggg
      481 aacttattaa attagttgaa ttacccgaag aatatttgga tcgctaccct agtgagttgt
      541 ctggcggtca gcaacaacgt atcggtgtca ttcgcgctct tgcagcagac caagatatta
      601 ttttaatgga tgagcctttt ggagctctgg atcctattac tagagaaggt attcaagact
      661 tagtcaagtc tcttcaggaa gaaatgggga aaactatcat cttagttact catgatatgg
      721 atgaagccct caagttagca acaaaaatta ttgttatgga caatggtaaa atggtccaag
      781 aagggacacc caatgatctc ttacatcatc ctgctactag tttcgttgaa caaatgattg
      841 gagaagagcg tctccttcat gcgcaggctg atattactcc tgttaaacag ataatgttaa
      901 ataatcctgt ttcaataact actgaaaaaa cactaactga agctattaca ctaatgcgcc
      961 aaaaacgcgt tgactcactt ctagtaaccg ataacggtaa attaattggt tttattgact
     1021 tagaatctct aagcagtaaa tataagaaag atcgacttgt ttctgatatc ttaaaacata
     1081 ctgattttta tgttatggaa gacgacttac ttagaaatac tgctgagcgt attttaaaac
     1141 gtggtttaaa atacgctcca gttgttgacc atgagaataa cttaaagggc attgttactc
     1201 gtgcatccct agttgatatg ttatacgata ttatttgggg cgatactgaa acggaggatc
     1261 aataatggtc aatttcttat cacagtatgg catgcaaata ttagtaaaaa catgggaaca
     1321 agtctatatc tcattttttg ctatagcact tgggattgca attgctgttc cattgggtgt
     1381 ggtcttaaca cgttttccca aagtagccaa aattatcatt gctatagcta gcatgctaca
     1441 aactatacca agtttagcct tattagcttt aatgattcca ttgtttggaa ttggaaaagt
     1501 cccagctatc gttgctttat ttatttattc cttgcttcct atactaagga atacttatat
     1561 aggtatgaat aatgtcaatc caacacttaa agattgtgct aaagggatgg gaatgaaacc
     1621 tattcaatct atatttcaag ttgagttacc tttagctact ccaattatta tggctggtat
     1681 tcgcttgtct actatttatg ttattgcttg ggctacatta gcttcttata taggtgcagg
     1741 tggacttggt gatctcattt ttagcgggtt aaatcttttt cagcccaaat taattctagg
     1801 gggcacaatt cctgttatta tcctgtcact tattatagat tatcttttag gattactgga
     1861 aacagctcta acgcccagaa caacaagaag ggaggcttag atatgcttaa aaaatcgcac
     1921 tttttacaga tatttacact ttgcttagcc ctcttaacga tttctggttg tcaattaacc
     1981 gatactaaaa aacctggtca taccacaatt aaggttgctg cccaaagttc tacagagtct
     2041 agtatcatgg caaatattgt caccgaatta attcatcacg aattaggata caacacaact
     2101 ttaataagca atcttggttc ctctacggtt actcaccaag ctttgctccg tggtgatgct
     2161 gacattgctg ccacacgtta tacaggaaca gacatcacag gaactcttgg cttaaaagct
     2221 gttaaagacc ctaaagaagc ttctaagatt gtaaaaactg aattccaaaa acgctacaat
     2281 caaacttggt atcctactta tggtttttct gatacttatg cattcatggt tactaaagag
     2341 tttgccagac agaataaaat caccaagatc tctgatctca aaaagttatc aacaactatg
     2401 aaggcagggg ttgatagttc atggatgaat cgcgagggag atggatacac tgatttcgct
     2461 aaaacatacg gttttgaatt ttcacatatt taccctatgc aaattggctt agtctatgat
     2521 gcggttgaaa gtaacaaaat gcaatctgta ttaggctact ccactgacgg tcgtatttcg
     2581 agctatgatt tagaaatttt aagggatgat aaaaaattct ttcctcctta tgaagcctct
     2641 atggttgtca acaattctat catcaaaaaa gatcctaaac taaaaaaatt actccatcga
     2701 ctcgatggta aaatcaattt aaaaacgatg caaaacctta attatatggt agatgataaa
     2761 cttttagaac cttcagttgt tgccaaacaa tttttagaaa aaaaccatta ttttagagga
     2821 gataaataaa tgaaacaaat gaatactttc caacaattta tctactactt ccaacataat
     2881 ggtagttata tcttagaaca atttatccac cactttttaa tttcaattta cggcgttatt
     2941 ttagctgcaa ttgttggtat ccctctaggt atcttcatcg ctagacgtgg taaaattaaa
     3001 acctttgtta tgggaatcgc aaatgtcatc caaaccattc cttctttagc tatgatctct
     3061 atcattatgt taggtctagg gctaggtatt aaaacagtag ttgcaactgt ctttctttat
     3121 tctcttctgc caatcatcac aaatacttac actggtatta gaaatgttga ctccgactta
     3181 ctagatgctg ctaaaggaat gggcatgact aagcgtcaac gtttatttat ggttgagtta
     3241 ccactatcta tttctgttat catggctgga cttagaaatg cccttgttgt agcaattggt
     3301 attacagcca ttggtgcttt cgtcggtgga ggcggacttg gagatatcat cataagagga
     3361 actaatgcaa caaatggtgg tgctattatc ttagcaggat ctcttccaac agcccttatg
     3421 gcaattttct ctgatttaat cctaggcggt atccaacgca tgcttgaacc aagaaaataa
     3481 ctattaaaaa cttgtcagta gagacaagtt tttttgatgt ttcagaataa tttcttaagt
     3541 tctaaaattg ttttttacta tagctaaatc ctaatcctcc caatatagca atggcacatc
     3601 ctataacaat aaagctaaca tttgataagg caaatgaaaa gatagtcata aatagtgttc
     3661 ctataggcat aaacataata gcaatagtaa atatagtcga catcacacgc cccatatacg
     3721 cttcatcgac cctgatttgt acctgactga aaaaatgaat attaaacatt gtcagaagac
     3781 tattaaaaag aaaactacca ctgtaagata tccatatcgg taattcaaat agagatggga
     3841 atcccataac tatgactccc aaactactga atactagcat agatagcata gaattaatac
     3901 tagatttaat ttttctagca ataagcgctc caataattga tccgatagct gatatactaa
     3961 gtattgttgc ataagcacct gaagttttca atagggagtt agtaaaaggt aaaagataat
     4021 tgaacattgc taggaaagtg tttaataaag cagcaataat tagaaaaaac ataatttctt
     4081 tttctttttt gatgtaagta aatccatctg caatatcagc taaaatattt tttatcgcta
     4141 ccttttcttg ttttgaaagc tgtacccttt ccactttaat agcataaaga aacaggaaag
     4201 agatcaaaaa agtaatcgca tcaacaatta aagtaatcct aataccaaaa ttattaaaaa
     4261 taaggaaacc taaaacaggg gaacttacac taataatttg cacaatggtt tccagattag
     4321 cattataggt aatgatatca gctttatcta ctatttcagg aatataagat ttataagccg
     4381 gagaagaaaa agcattggaa accgctaaga tagcattagc aactattaga ccatacacca
     4441 ttactttatc atctccaata aaagaaagta agaaacacat gatagcacag atagcatctg
     4501 ttattagtaa aatttttctt cgttgaaagc gatcagctaa ggcgcctcca aaagggttga
     4561 ggacaataga aaccaacaac tctactatct gataaatacc taaaatcttt tgtcctagac
     4621 cacctatcga agcaatccaa gtactatttc cataatcata aagcacatca ccaagtttac
     4681 tgactgctcg actagatagt aattttatac cattaatatt cataattccc tccttagttc
     4741 gacattatag caaaccaaaa ggagaaatat taaaaaatcc cataaatggg attttttata
     4801 atcttgttac cctattaaag tgttcctgaa aattttcaac atgatgttta gaaccaattg
     4861 cttggaagca aaaaatagct tgcttcatgc tttgtattcc tcgactatcc cctttagcat
     4921 aggcaagaaa tcctttttca tagagtaaaa taatacggtg ataagcattc cgctcattat
     4981 ttaaaagtgc ttcaactttc tctagaaaaa acgttgcttc ttctagagcg cgccgttcta
     5041 ttagtgtctc taccacattt aggagggttg cttcgacaac attgcggttt gtataaatat
     5101 ctcgataata ttccttttga ttgataacct ctttaacaag ttggcaaata tgccttgtgt
     5161 tgtaataccg gtaaagattt ccaatcaaaa tatagtcact aatttcccaa ctatcctttt
     5221 gaaagaggta atccgacaaa aagcaaaggt cttcttccga catttttctt gaggagtcat
     5281 attcacatat cattcctcta aaaagaacta tgtttaaacg acaacgtata tctgaactat
     5341 ccttttttag tttaccttct tccacagaaa tcatcttttc taacccagct acgtctcttt
     5401 gataatgata ttgagccatc tgagacatta ctgatcagat ttttgatatt tatttactct
     5461 atccataaat tcgctaattg ataaatcaat tgcctctaaa gcccctaaaa attttgttaa
     5521 tgataaatct gattcacccc tctcaaatcg tgataactga gaaacagaaa gttcatttga
     5581 agcaacctgt ttaagagaaa actgtcgatt aagtcgaaat tccctaaaaa tagggccata
     5641 tataaacatt agtattcctt tctaaccgta atctcattgg taaacttatg aggtaatttc
     5701 aataatttat atttaccaac ttta
//
LOCUS       DATRSG010000086         5683 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.087, whole genome shotgun sequence.
ACCESSION   DATRSG010000086 DATRSG010000000
VERSION     DATRSG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.087"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            266..1408
                     /gene="tgt"
                     /locus_tag="VBD89_001845"
     CDS             266..1408
                     /gene="tgt"
                     /locus_tag="VBD89_001845"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO7136347.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            1514..1825
                     /locus_tag="VBD89_001846"
     CDS             1514..1825
                     /locus_tag="VBD89_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136348.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            1832..2371
                     /locus_tag="VBD89_001847"
     CDS             1832..2371
                     /locus_tag="VBD89_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO7136349.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYMNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            2510..3286
                     /locus_tag="VBD89_001848"
     CDS             2510..3286
                     /locus_tag="VBD89_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7136350.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            3286..3792
                     /locus_tag="VBD89_001849"
     CDS             3286..3792
                     /locus_tag="VBD89_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO7136351.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            3827..3912
                     /gene="ffs"
                     /locus_tag="VBD89_001850"
     ncRNA           3827..3912
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBD89_001850"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4064..5413
                     /locus_tag="VBD89_001851"
     CDS             4064..5413
                     /locus_tag="VBD89_001851"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO7136352.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
BASE COUNT         1832 a          899 c         1126 g         1826 t
ORIGIN      
        1 ggagtaaaaa aagttgattc ttaccttgaa gattgcttaa tatagttttt gcctcatttt
       61 taatttctga attcgtcata tctcctccat acataaaagt acaattcatt tcattataac
      121 acattcctta agatgagcat gacaagagta catttttttg ataaaatctt aatggctaaa
      181 aaagaaatta atacaaagta ttacatttag atattgatta gcactaaagc ccatgactgt
      241 ttttcatgga agcaaggaga aaacgatgac tgatcatcca ataaaatatc gattaattaa
      301 acaggaaaaa cacacaggag ccagacttgg tgaaataatc acaccacatg gtacatttcc
      361 gacaccaatg tttatgcctg ttgggacaca agcaactgtt aagacacaat ctcctgaaga
      421 actaaaagaa atgggttcag gaattattct atcaaatacc taccatttat ggttacgtcc
      481 aggtgatgaa ttgattgcta aagctggagg tttacataaa ttcatgaact gggatcaggc
      541 tattctaaca gacagtggag gctttcaggt ttattctttg gctgatagtc gaaacatcac
      601 tgaagaagga gtaaccttta aaaaccattt aaatggtgcg aaaatgtttt tatcaccaga
      661 aaaagctatt tcaattcaaa ataaccttgg ttctgatatt atgatgagtt ttgatgagtg
      721 tccacaattt taccagcctt atgattatgt aaaaaaatca attgagcgaa caagccgttg
      781 ggcagaacgt ggattaaatg cccaccgccg tcctcatgat caaggactat ttggtattgt
      841 tcaaggggca gggtttgagg atttacgtcg tcaatcagca cgagatttag tatcgatgga
      901 ttttccaggt tattcaattg gagggcttgc ggtcggagaa acacatgatg agatgaatgc
      961 agttcttgat tttactgttc caatgcttcc gaatgataag cctcgttatt tgatgggagt
     1021 tggtgcacca gatagtttaa tagacgccgt tatccgagga gtagatatgt ttgactgcgt
     1081 cttacctact cgtattgctc gaaatgggac atgtatgaca agtcaaggtc gtttagttgt
     1141 taagaatgct aaatttgcag aagattttac gcctcttgat ccaaattgtg attgctatac
     1201 ttgtaaaaat tatacacgcg cttatatccg tcacttattg aaagcagatg aaacttttgg
     1261 tattcgtttg actagttacc ataatcttta tttccttgtc aatcttatga aagatgtcag
     1321 acaagctatt atggatgata atcttctaga gttccgtcaa gattttatgg agcgctatgg
     1381 ttatggcacg aataaccgta atttttaaaa aagttcctgg taaaacaggg ctttttttga
     1441 tctgtttgtt aaccgcgttt taaaaaaagt tgacaataga aaaacttttg tataatatgt
     1501 tataggagga atcatgatgc aagagtactt tggtattggt cttgatagta gcagccgctg
     1561 ttaccactat catactaaat tggatattgt cgctttaaaa tgtgctgttt gtcaaaaata
     1621 ttatgcttgt tataaatgtc atgatgcact cgaggagcat tgttttgcag caacaaaatc
     1681 tgatgaaatg tttcctgtct tatgcggttc ttgtcgtcag atgttgacat taaaagagta
     1741 taaaactggt ttttgtcctt attgtcgtat gctttttaat ccaaattgtc aacgccatga
     1801 ttctatttat ttttcgaagg agtaattttt aatgtttcac acaaaaagcc taatccagat
     1861 ggctatgatg gctactttat tggtggtact tggtttcatt cctgctctac cacttggttt
     1921 cattccggtc cctatcgtct tacaaaacct aggggttatg atggctggtg ttattttggg
     1981 cggaagaaaa gggagtttat ctattttgct cttctttctt gtcggtttag ttattcctgt
     2041 attctcaggt ttacgttcga ctatacctgt attgacagga ccaacagctg gttatgttat
     2101 tgcttggttg tttgttcctt tgcttatctc atatggtgta aaattagtaa ataaaaagaa
     2161 ttttatagct attttcctta ttgtatggct agcaggggtc ctttttgtag atgtagcagg
     2221 agcagtttgg ctggcatctt atatgaacat cccattagat aaatcacttc ttcaaaactt
     2281 agtatttctc ccaggagata caataaaagc cattatagct gctgttgttg ctgttaaata
     2341 taaagatagt tttttcttga ctgaaaaata atcaagattg ctatatttta gatggactag
     2401 tagatctaaa aatttagatt tactagtctt ttttgtgcat aaaatctaaa acagttattc
     2461 ttaacaaaat ttggtataat agcattaaat cagtaaggag gggattatca tgaaattaac
     2521 aacgttaggt tgttggggtg gttatcctta taaagatgga ggaaccacaa gttttctatt
     2581 aactggagaa aataattttc acttattaat agatgcggga agtcgttcaa tcaatgagtt
     2641 agaaaaagaa ataagcccgt tggatttgga tgcagttatc atcagtcatt atcatccaga
     2701 tcatgtagca gatttgggtg tgttgtatca ttatcgtttg ctatacccta aagataaatg
     2761 ggaacccaaa gtattgccag tttatggaca taatgaggat gattatgagt tttctaaatt
     2821 gacattacct aacgttgctc aaggaattgc ttatcatacc aatggagttg agcaagttgg
     2881 tcccttcaat attacgttta tcaaaacagt ccacccagtc gtctgctatg ccatgaggat
     2941 tacagaaaga gaaactggtc aagtattagt ctttacagga gattctgggt attttgctga
     3001 ccttgtacct tttacaaaag gcgcagattt atttttagca gacgtttatc tttacgaggg
     3061 aaatgaaaat catattgcac atttgactag taaagaagca ggtgagattg cttataacgc
     3121 taaagttaag aaattagttt taacacatgt tccgcagttt ccgggagaag gaataaaccc
     3181 cgaaaatcat ttggaagaac ttcgactaca gtctgaaaag tatgcaggtg gcatctctgt
     3241 tgatttagct cagccacata aatcttggga cttaggaaaa ctataatgca gaaatttacc
     3301 caagaagaaa aagaatggtt tatgacagaa gctctaaagg aggcaaaatc ctccttagag
     3361 aaagaagaaa ttcctatagg ttgtgtcatt gtaaaagatg gtcatattat tggaagagga
     3421 cataatgctc gtgaagagtt taataaagct attttacatg ctgaaatcat ggctattaac
     3481 aatgcaaatg agaaggtagg gaattggcgc ttattagata ctactttatt tgttactgta
     3541 gaaccttgtg taatgtgtag tggagctatt ggcttagcta gaattcccca cgttatatat
     3601 ggcgctaaga atacgaaatt tggagcagcg ggaagtctct atgatatttt aagtgatagc
     3661 cgacttaatc atagagttga agttgaaacc ggcattttag aagatcaatg cgctaaaata
     3721 atgcaggatt tctttcgaaa aggacgacaa cgcaagaaag aagctaaact aagtgaaaaa
     3781 actaagattt gatagcttgc aataaaatat tagttttgtt ataatatctt tggagcaaca
     3841 tttactcgtg aagtgggtca ggggaggaat ccagcagccc taagcgatgc taaatgtgtg
     3901 ctctttttat tttcaaaagt caaagtgttt ttggaaataa ttatgaaagt ttcataaaaa
     3961 ttggtagcgt ttcaaaaaat atgaaaatac agttaaaaaa ccttgaaaaa aaactcacaa
     4021 aagagtaaga tgatagtgaa acataattgt taggagaaat aaaatgacac atattacatt
     4081 tgactattca aaagtcttgg gtcaatttgt aggcgaacac gagttagact acctacaacc
     4141 acaagtaagt gcagcagatg ctttccttcg tcaagggact ggtcctggat cagattttct
     4201 cggatggatg gacctcccag aaaactatga caaagaagaa ttttctcgca ttcaaaaagc
     4261 cgctgaaaag attaaatcag atagcgaagt actcgtggtt attggtattg gtggttcgta
     4321 ccttggtgca aaagcagcaa ttgacttttt gaataatcat tttgctaatt tgcaaaccgc
     4381 agaagaacgt aaagcgcctc agattcttta tgctggaaat tctatttcat ctacttacct
     4441 tgccgattta gttgaatacg tccaagataa agaattctca gtaaatgtca tttcaaaatc
     4501 aggtacaaca actgaaccag cgattgcttt ccgtgtattt aaagaacttc tagttaaaaa
     4561 gtacggtcaa gaagaagcta ataaacgtat ctacgctaca actgataaag ttaaaggtgc
     4621 cgtaaaagtt gaagcagatg ctaacaattg ggaaacattt gtagttcctg ataatgttgg
     4681 tggtcgtttc tcagtactga cagctgtagg tcttcttcca attgctgctt caggagcaga
     4741 tattactgct cttatggaag gcgcaaatgc tgctcgtaaa gatttatctt cagataaaat
     4801 ctctgaaaac attgcttatc agtacgctgc agttcgtaat gttctttatc gcaaaggtta
     4861 tatcacagag attttagcta actacgaacc atcattacaa tactttggtg aatggtggaa
     4921 acaattagca ggtgaatcag aaggtaaaga tcaaaaaggt atctatccta catcagcaaa
     4981 cttctcaact gatcttcact cacttggtca atttattcaa gaaggttacc gtaacctctt
     5041 tgagacagtt gttcgcgttg agaaacctcg taaaaatgtg actattcctg aattaaccga
     5101 agatttagat ggtcttggtt atcttcaagg aaaagatgta gattttgtta ataaaaaagc
     5161 aacagatggt gtgcttcttg ctcatacaga tggtggggtt ccaaatatgt ttgtaacgct
     5221 tcctacacaa gacgcttaca ctcttggtta cactatttac ttctttgagt tagcaattgg
     5281 cctttcaggt tatcttaact cagtaaatcc atttgatcaa ccgggggtag aagcatataa
     5341 acgtaatatg tttgctcttc taggtaaacc tggattcgaa gagcttagcg ctgaattgaa
     5401 tgcacgtctt taaaaatatc taactaaaaa gttcctcaaa gagaggaact ttttagtttt
     5461 gttgtaggat aactaaaaac ccactagcac tggtagcgat agtgggttgg aaaaagggtt
     5521 gagaaaagtt attaactttt ctcaggagat ttatgaaaaa ggaaaataat ttaggataat
     5581 attttcctaa cggctaggat aaaccgctta ttttaggatg atgctagtat acagtagaaa
     5641 ccttaaataa accttaaaaa ttcatacttt atcattttat ttt
//
LOCUS       DATRSG010000087         5530 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.088, whole genome shotgun sequence.
ACCESSION   DATRSG010000087 DATRSG010000000
VERSION     DATRSG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.088"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..505
                     /locus_tag="VBD89_001852"
     CDS             <1..505
                     /locus_tag="VBD89_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO7136353.1"
                     /translation="ISVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVS
                     FPIPHFYGLKLEAYQKAMTVINKRKVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWK
                     AYQNNKGIKIFKAQKGFMKINIPKGKGKVTLIFIPYGFKFGVGLSITGIVLFTVYYLK
                     FGKNKIG"
     gene            complement(573..646)
                     /locus_tag="VBD89_001853"
     tRNA            complement(573..646)
                     /locus_tag="VBD89_001853"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(610..612),aa:Asn,seq:gtt)
     gene            complement(676..747)
                     /locus_tag="VBD89_001854"
     tRNA            complement(676..747)
                     /locus_tag="VBD89_001854"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Glu,seq:ttc)
     gene            939..1012
                     /locus_tag="VBD89_001855"
     tRNA            939..1012
                     /locus_tag="VBD89_001855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:973..975,aa:Arg,seq:tct)
     gene            complement(1085..1564)
                     /gene="rlmH"
                     /locus_tag="VBD89_001856"
     CDS             complement(1085..1564)
                     /gene="rlmH"
                     /locus_tag="VBD89_001856"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO7136354.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            1765..2994
                     /locus_tag="VBD89_001857"
     CDS             1765..2994
                     /locus_tag="VBD89_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO7136355.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            3092..3865
                     /locus_tag="VBD89_001858"
     CDS             3092..3865
                     /locus_tag="VBD89_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO7136356.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFNNQEELNRIINTLK"
     gene            4074..5435
                     /gene="dnaA"
                     /locus_tag="VBD89_001859"
     CDS             4074..5435
                     /gene="dnaA"
                     /locus_tag="VBD89_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO7136357.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT         1955 a          947 c          922 g         1706 t
ORIGIN      
        1 aatatccgtt aataatggac catttgtacc taatactctt gataatgctt attcattttt
       61 taatattggt tcctttgctg aaaacagtcg tatcaaagta aaatttcaat ttccacacaa
      121 tgatcaagtt tctttcccaa ttccccactt ttatgggctc aagttagaag cttatcaaaa
      181 agctatgact gttattaata aacgaaaagt aaaagtaaga actgatcaca ataaagttat
      241 tgctaattat acaagtccta atagaagttc tcttttcttc actattccct atgatagagg
      301 ttggaaagct tatcaaaata ataaaggaat aaaaattttc aaagctcaga aaggttttat
      361 gaaaataaat attcctaaag gaaaaggcaa agtaacatta atttttatcc cttacggctt
      421 taaatttggg gtaggcctat ctattactgg aatagtatta tttactgtct attatttaaa
      481 atttggaaaa aataaaatag gatgagagta ctctcatcct attttattaa caaaaaaacg
      541 ccgacaaatc aacgtttaga tgtgtcttat cactccggca gcaggactcg aacctgcgac
      601 atcatgatta acagtcatgc gctactacca actgagctat gccggataaa taaaaaactg
      661 caaagcagtt atcaatagtc cgtacgggat tcgaacccgt gttaccgccg tgaaaaggcg
      721 gtgtcttaac cccttgacca acggaccata ggttaggtgt ttccttagta ctcttaatat
      781 tatatcagaa aaaaaaggtt tgtctatact tttgttcaat aatttttaca aaaaaattga
      841 acctacttat gagcaaaaaa atatatttcc aactcataaa tagtacatat gaaaacttga
      901 cagcctgcct aatattttgg tagaataaca gagttgatgg ttccatagct cagctggata
      961 gagcattcgc cttctaagcg aacggtcgca ggttcgaatc ctgctggaat cagataatga
     1021 aagtctagat agattatcta ggctttttta tttgttttta gcggttgttt cataaatgga
     1081 acgattattt gtggtaagga gtgccttgac gaatcataaa agcacgataa atttgttcca
     1141 tcaagactaa tttcataagt tgatgaggta aggttaacag tccaaaactc attaaataat
     1201 cagcacgttt tttcactttt tgagataaac ccaaactacc accaataaca aaagtaatag
     1261 tagagtagcc ggatatcatt ttttgatcga ttagatggct aaatgattca gatggcaatt
     1321 gtttcccttc tatagccaaa ccaatgacat aatccctctc acctatttta ctcaaaatac
     1381 gttccccttc tatatctaaa attctttgat tttcagatat actagcttta tcaggtgtct
     1441 tttcatcagc tagctcaatt gtctcaattt tagaaaagcg attcagacgt ttctgatact
     1501 ctgcaacacc ttctttaagg tatttctctt tcaattttcc aactgtaatt atttttaatt
     1561 tcatagctac attataacat aatccacaat attttcacaa tttatccaca tgtttatcct
     1621 tacatatccc cttattttat aggcaatcta taactataat caaggttatc cacaacttgt
     1681 ggatatttcc tttttttagc ttttaaggta taattaagaa gcttgtcata agattagtat
     1741 aaaaatttac ggaggtagct atttgtgaaa aaaaaattag tctcatcact tctaaagtgt
     1801 tctctaatca ttattgttag ctttgctggt ggagcatttg ctagttttgt catgaatcat
     1861 aatgacaata ttccaaatgg tggtgtcact aaaactagta aagtaaatta taataacata
     1921 acgcctacaa caaaagctgt taaaaaggta caaaatagtg ttgtttctgt tatcaattat
     1981 aaacaacaag agagtcgttc tgacctatca gacttctata gtcatttttt tggtaatcag
     2041 gggggcaaca ctgataaggg cttacaagtt tacggtgaag gctctggagt catctataaa
     2101 aaagatggta aaaatgccta tgttgtcact aataaccacg tcattgatgg ggctaaacaa
     2161 attgaaattc aactagctga tggctcaaaa gcagttggga aacttgttgg gtcagatacc
     2221 tactctgatt tagccgtcgt caaaattcca tcagataaag tttcaaatat tgcagaattt
     2281 gctgattcat caaaactcaa cattggtgaa actgctatag cgatcggaag ccctcttgga
     2341 actgagtatg caaattctgt aactcaaggt attgtatcta gtttaaaaag aactgtaaca
     2401 atgactaatg aagaaggaca aacagtttct acaaatgcta tccagacgga tgctgctatc
     2461 aatcctggta attcaggtgg agcacttatc aatattgaag gacaggttat tggaattaat
     2521 tctagtaaaa tttcttctac atcaaatcaa acctcaggac aatcgtcagg aaatagcgtt
     2581 gaaggtatgg gatttgccat tccttcaaat gatgttgtta agattatcaa tcaacttgag
     2641 agtaacggac aagtagagag acctgctcta ggtatttcta tggctggatt aagtaattta
     2701 ccatccgatg ttattagtaa actgaaaatc ccaagtaatg ttactaatgg tattgtagta
     2761 gcatctatcc aatctggcat gccagctcaa ggcaaactaa agaaatacga tgtcattact
     2821 aaagttgacg ataaagaagt agcatctcca agtgatttac aaagtttact ctatggccac
     2881 caggtagggg attccataac agtaaccttt tatcgtggtg aaaataaaca aacagtcact
     2941 ataaaactta ctaaaactag taaagattta gctaaacaac gagcaaataa ctaaatcaat
     3001 tctagttatt ttaactaaga tatagtaaaa ttaaaggggt tggtcagata gaccaatccc
     3061 tttagtaagt ctatagaatc tgaggtctaa aatggaatat ttagaaacga tcaacatcaa
     3121 tcatattgct ccaaatccgt atcaacctcg cctagaattt aatacaaaag aactagaaga
     3181 attagccgac tctattaaaa ttaatggcct tattcaaccc attattgtaa gaccgtcagc
     3241 agtctttggt tacgaactgg tagccggaga acgacggtta agagccgcaa agttagctaa
     3301 attggaaagt atacctgcaa ttataaaatc ctataataat gatgatagta tgcaattagc
     3361 tattgtcgaa aatttacaac gatctaattt gtcacccatt gaagaggcta aagcctactc
     3421 tcaacttcta caaaagaaaa gtatgacaca tgaagagtta gcaaaatata tgggaaaatc
     3481 tcgtccctat atatcaaata cgatacgact actaaattta ccgccactaa ttacttcagc
     3541 tattgaagaa ggaaaacttt cctctggtca tgcgcgtgca ctactcagtc taccagatgc
     3601 tagtcaacaa aaagattggt atcagagaat cctcactgaa gatattagtg taagacgact
     3661 agagaagtta ttaaaacaag aaaagaaaac aaatcataag tcacttcaaa ataaagatgt
     3721 ctttctaaaa catcaagaaa atgagttagc tcaatttcta ggtagcaaag tgaaacttac
     3781 tattaataaa gatggtgctg gcaatataaa gattgctttc aataaccaag aagaattaaa
     3841 cagaattatc aacactctaa aataagggtg ttgattattt ttttatttaa tcaaacttat
     3901 ccacaaggta ttttgctatt tttctgttga ttctctaagc ttttctaatt tccacagtct
     3961 gtggaaaact ttaattaaca ttgttgattt tattcttcaa catctgtgga aaactttatt
     4021 tttttatggt acaatataac aataattatc cacaagacaa taaggaagaa gctatgacgg
     4081 aaaacgaaca actattttgg aatagagtac tagagctatc tcgttctcaa atagcaccag
     4141 cagcttatga attttttgtt ctagaggcta gactcctcaa aattgaacat caaacggcag
     4201 ttattacttt agataacatt gaaatgaaaa agctattctg ggaacaaaat ttggggcctg
     4261 ttatcctaac agctggtttt gaaattttca atgctgaaat tacagctaac tatgtctcaa
     4321 acgatttaca tttacaagaa actagttttt ctaactacca gcaatctagc aacgaagtaa
     4381 atactttacc aattagaaaa atcgactcta accttaaaga gaaatatact tttgctaatt
     4441 ttgttcaagg agatgaaaat agatgggctg tttcagcatc aattgctgta gctgatagtc
     4501 ctggcacgac ttataatcct ctatttatct gggggggacc tggtctagga aagacgcatc
     4561 tactaaatgc tattggaaat caagttttaa gagataatcc aaacgcgagg gttttataca
     4621 tcactgctga gaattttatt aatgaatttg tcagtcatat tcgtttagat tcgatggaag
     4681 aattaaaaga aaagtttcgc aacttggact tactcctgat tgatgatatt cagtcgcttg
     4741 ctaagaaaac cttagggggg acccaagagg agttcttcaa tactttcaat gctttacata
     4801 caaacgataa acaaatcgta ttgaccagtg accgaaatcc aaatcaatta aatgatctag
     4861 aagaacgtct agtcacgcgc tttagttggg gactcccagt aaatatcaca ccacctgatt
     4921 ttgaaacacg agttgctatt ttaaccaata aaattcaaga atatccttat gattttcctc
     4981 aagataccat tgaatactta gcaggagaat ttgattccaa cgtacgtgaa ttagaaggag
     5041 ccttgaaaaa tattagtcta gttgctgact ttaagcatgc taaaactatt acagtagata
     5101 tagctgcaga agctatcaga gcacgtaaaa atgacggtcc tattgttact gttattccta
     5161 tagaagaaat tcaaatacaa gttggtaaat tctatggcgt aactgtaaaa gagataaaag
     5221 caactaaaag aacacaagat attgtccttg caagacaggt agccatgtac ttagctcgtg
     5281 agatgacaga taacagtctc cccaaaatag gtaaagaatt tgggggacga gatcactcaa
     5341 ctgttctcca cgcttataat aaaataaaaa atatggttgc tcaagatgac aacttacgaa
     5401 ttgagataga aactatcaaa aataaaatca ggtaggcttt gtggataagt atcagaaaaa
     5461 cacgagactt ttccacaggt tgtgaacaag ttattttcct tatgaatact aaagttataa
     5521 tacttattca
//
LOCUS       DATRSG010000088         5479 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRSG010000088 DATRSG010000000
VERSION     DATRSG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.089"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            28..1194
                     /locus_tag="VBD89_001860"
     CDS             28..1194
                     /locus_tag="VBD89_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7136358.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            1300..2169
                     /locus_tag="VBD89_001861"
     CDS             1300..2169
                     /locus_tag="VBD89_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7136359.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            2172..3125
                     /locus_tag="VBD89_001862"
     CDS             2172..3125
                     /locus_tag="VBD89_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7136360.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            3126..3890
                     /locus_tag="VBD89_001863"
     CDS             3126..3890
                     /locus_tag="VBD89_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136361.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            3890..4600
                     /locus_tag="VBD89_001864"
     CDS             3890..4600
                     /locus_tag="VBD89_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136362.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEQSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            4619..5278
                     /locus_tag="VBD89_001865"
     CDS             4619..5278
                     /locus_tag="VBD89_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7136363.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
BASE COUNT         1713 a          822 c         1185 g         1759 t
ORIGIN      
        1 ttaccatttt caaaaaagga gaaaaatatg gaaaagagac ttagtttagg agcgcttgtt
       61 ttagctagta cagttctttt agcagcatgt ggaaatgtcg gaggaggagc atcttcgaca
      121 ggaacaaaaa tagggaaaga tattaaggta gggtataact gggaattatc aggaaacgtt
      181 tcttcatatg gaaattcaat gaagaatgga gctgacttag cagtaaaaga aattaatgct
      241 gctggagggg ttggcggcaa gaagttaaaa gtcctatcac aggataataa atcagaaaat
      301 gcagaggcag caacggttgc cacaaacttg gttaccaaag gagctaatgt cattatcgga
      361 ccagcaacat cgggtgcagc tgcatcttca actccaaaag taaatgcagc agcagttcca
      421 atgattgcac ctgctgcgac acaagacaat ttagtctatg gttctgatgg aaaaacctta
      481 aatcagtatt tcttccgagc tacttttgtc gataattatc aaggaaagct attgtctcag
      541 tatgctacag acaaccttaa agctaaaaaa gttgttctat tttatgataa ttcatcagat
      601 tactcaaagg gggtagcaaa atcatttaag gaaagttata gtggaaaaat tgttgatagt
      661 atgacattct ccgctggtga tactgatttc caagcgtcat tgactaagtt gaaagggaaa
      721 gaatatgatg ctattgtgat gccaggttac tataccgaga caggattaat agttaagcaa
      781 gcgcgtgatt taggtatctc taaaccggtt cttgggcctg atggttttga tagtccgaaa
      841 tttgtgcaat cggcaacacc tgtaggagct tcaaacgttt attatttgac aggtttcact
      901 acacaaggat caaccaaagc taaagctttc catgatcatt acgttaaggc atatggtgaa
      961 gaaccatcca tgttctcggc tttatcatac gacgctgtgt atatggcagc taaatctgct
     1021 aaaggagcta aaacttcaat tgatattaaa aaagctttag ctaagctgaa agattttaaa
     1081 ggtgtgacag ggaaaatgtc tattgataaa aatcacaatg ttgttaaatc agcttacgtt
     1141 gtcaaattag aggatggaaa aacaagtagc gttaacatta tttcagcaaa ataagtattc
     1201 tctagaaaaa tgggagttta attttcagct cccctttttt aaaaagtatt ttttcagaat
     1261 aattcgacaa taatatataa tagaaagtat ggttataata tgcttcaaca gctcgttaat
     1321 ggacttatct taggatccat atatgcttta cttgccttag gatatactat ggtatatggc
     1381 attattaagc tgattaattt tgctcatggg gacatctata tgatgggcgc tttcatgggc
     1441 tattatttga ttaatcatct tcatcttaat tttttcctag ctttattaat tgcaatgctg
     1501 gggtctgctt tcctaggtgt tgtaattgaa tatttagctt atcgtccact gagaaaatca
     1561 actagaattg ccgcgttaat tacagctatt ggtgtttctt ttcttttgga gtacgggatg
     1621 gtataccttg ttggagcgga tactagggca tttccgcaag ccattcatac tgttaaatat
     1681 aatttaggac cgataactat aaccaatgtt cagctcatca ttttaggtat tgcgctatta
     1741 ctaatgctta ctttacagtt catcgttcaa aaaactaaaa tggggaaagc tatgagagct
     1801 ttatcagtgg atagcgatgc cgcacaattg atgggaatta atgttaaccg taccattagt
     1861 tttacatttg cgctggggtc tgctttagct ggagcaggtg gagtactgat tgggctttat
     1921 tataattctg ttcaacctct tatgggggta actccagggt tgaaagcatt tgttgcagct
     1981 gtcttaggtg gtattggtat cattccggga gcggctatcg gtggttttgt tattggcatt
     2041 ttagaaactt tagcgactgc acttggtgtg tcagattttc gagatggtat cgtttatgct
     2101 attttaattc tcattttctt gatccgtcca gcaggaattc ttggaaaaaa tattaaggag
     2161 aaggtctagg catgaaagaa aatatgaaat caattttatc ttggttagct atagtagtgt
     2221 taggttatct cttgattagc gtgctaatat ctatgggtat atttaacctc tatcatattc
     2281 aaattattga aacaattggg attaatgtta ttttagcagt tggtttaaac ctaattgtag
     2341 gttgttcggg acagttttca ctaggacatg caggtttcat ggctattggc gcttatgcag
     2401 ttgctattat cggcgttaaa atgccaactt atgtaggttt tttgatagct atacttgttg
     2461 gaactcttgt tgcaggaggt attgccttag gtgtaggaat tcctactttg agactaaaag
     2521 gtgattattt agctatagct actttaggtg ttgctgagat tatccgtatt ttgttggtta
     2581 atggaggtga tattacgaat ggtgctgcgg gtatcatggg gattccgcct tttacaacct
     2641 ggtctttagt ttatggggtg gcagttgtga gccttatcct tgcaatgaat tttttgcgta
     2701 gtcccttagg gcgtaataca attgctatac gagaagatga aattgcagca gagtctatgg
     2761 gtgtggatac aacaaaagtg aaagtaattg tttttgtttt tggagctatt ttagcttcta
     2821 tcgctggtag tttacaggct ggctacgttg gaactgttat gccaaaagac ttttcattta
     2881 tgatgtctgt caatgtttta attattgttg ttttaggtgg tttaggttca atgacaggga
     2941 cagttttagc agcaatttta ttaggattat tgaatatgtt actacaagac tacgcttctg
     3001 ttcgaatgat tatctatgct ttagccctga ttttaattat gatttttaga cctagcggtt
     3061 tattaggaac gaaagagtta actctatctc atctatttcg taagacgagt aagggaggca
     3121 attagatggc acttcttgaa gtaaaaaatt taagtaaaca tttcggtggc ctaactgctg
     3181 taggtgatgt ttcgatgaaa ttacataaag gtgaattaat tggcttaatc ggccccaacg
     3241 gtgcgggtaa gacaacgctt tttaatctct taacaggagt ataccttcct agtaaaggga
     3301 caatatcact tgatggtaag atattaaatg ggaggaaacc agctaaaata gcttctttag
     3361 gtttaggtag aacttttcaa aatatccgac tttttaaaaa tatgactgtt ttagacaatg
     3421 tactagtagg actcagtaat catcacttat cacatcctat tgctagtttt ttacgattac
     3481 ccaaatacta tcatagtgaa aaagccttaa ggaaaaaggc actggagcta ttagagattt
     3541 ttggtttgaa agcatatcaa gatgctcttg ctaagaacct cccttatgga aaacaacggc
     3601 gtttagaaat tgttcgtgct ttagcaacag aaccaaaaat attgtttttg gatgagcccg
     3661 cggcaggaat gaatcctcaa gaaactgcag aattgacgca gttaattagt caaataaaga
     3721 gtgattttga tattactatt atgctgattg aacatgatat gaacttggtt atgcaagtaa
     3781 cagagagaat ttatgtattg gaatatggtc gtctgattgc tcacgggaca cctgaagaaa
     3841 ttaaaaataa taaacgtgtt atagaagcat acttgggggg ggagctataa tgaccatgtt
     3901 gaaagtagaa aatctatcta ttcactatgg tgttattcaa gcggtgaatg atgtctcgtt
     3961 tgaagttaac caaggtgagg ttgtaacctt aattggagcc aatggtgcag gaaagacatc
     4021 aattttgagg acaatttctg gtttagtaag accaagtcag ggttctattt cttttatggg
     4081 gaaacctata cataagttag cagctagaaa aattgttggc aatggtttag ctcaggttcc
     4141 agaagggcgc cacgttttct caagtttgtc tgttatggaa aatttagaaa tgggagcctt
     4201 tcttcaaaag gatcgtgaac aaaatcaaaa aatgttaaaa aaagtgtttg atcgttttcc
     4261 tcgcttagaa gaacgtaaaa accaagatgc agcaacctta tcaggtggtg agcagcaaat
     4321 gctcgctatg gggcgtgcat tgatgagtcg tcctaaatta ttacttttag atgaacagtc
     4381 aatgggccta gctccaattt ttattcaaga aatttttaat atcattgaag acatcaaaaa
     4441 acaagggact acagttcttt tagttgaaca aaatgcaaat aaagctctga cgattgctga
     4501 taaagcatat gttctagaga ctgggaaggt tgttttatca gggacaggta aggaattact
     4561 agtttcagat caagttcgta aagcttactt aggtggataa tatttagagg agtcaggtat
     4621 gccagtgaaa gattttatga caaaaaaatt agtttatgtc tctccagata ctacagttgc
     4681 agaagctgct gatttattga gagaacacca cttaaggcgt ttgccagttg ttgaaaatga
     4741 tcagctagtg gggttagtga cagaagggac aatggcagaa gcacaacctt ctaaagcaac
     4801 cagtctttct atatatgaga tgaactatct tcttaataag acaaaaattc gagatattat
     4861 gattaaagat attgtgactg tttcacagta tgctagcctt gaggacgcta tttatttgat
     4921 gatgtctcga aagatcggtg ttttaccagt agttgataat ggacaacttt atgggattgt
     4981 cacggatagg gatgttttta aagccttcct tgaaattgcg gggtatggtc aagaaggtta
     5041 tcgcttggtt atattagctg atgaggggat aggggtacta tcaaaagtcc taaatcgtct
     5101 ttctagtgca aatttaagtg ttaaacgttt agtcattatt gaacgaaaag caggtaagaa
     5161 agcggtcgag atacaactag aaggatacgc tgacaaggat gttttaaaac aagaactagt
     5221 atttgatgat gttattgtgg aaactttaga aaagaccatg aaaaaaccac tttcttaaaa
     5281 atatcagcta aattctcaaa gtaagttcca cctctagtag aaatggaatt tagtctgata
     5341 aaaaccaata aaaaccaacg aaaatccgtc tcagactaag ttccgttggt ttttataata
     5401 cttgatttca taggttttca gagttaaaaa tcgcaaaaaa agggtacaag aaaacatcct
     5461 catctgaaag cgtttcaga
//
LOCUS       DATRSG010000089         5475 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRSG010000089 DATRSG010000000
VERSION     DATRSG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.090"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..156)
                     /gene="secE"
                     /locus_tag="VBD89_001866"
     CDS             complement(<1..156)
                     /gene="secE"
                     /locus_tag="VBD89_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO7136364.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMD"
     gene            complement(192..344)
                     /gene="rpmG"
                     /locus_tag="VBD89_001867"
     CDS             complement(192..344)
                     /gene="rpmG"
                     /locus_tag="VBD89_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7136365.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(393..2714)
                     /locus_tag="VBD89_001868"
     CDS             complement(393..2714)
                     /locus_tag="VBD89_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7136366.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRLEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGQLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYKYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            2758..3642
                     /locus_tag="VBD89_001869"
     CDS             2758..3642
                     /locus_tag="VBD89_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7136367.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKVLNNYKKGVG"
     gene            complement(3667..5307)
                     /locus_tag="VBD89_001870"
     CDS             complement(3667..5307)
                     /locus_tag="VBD89_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136368.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
BASE COUNT         1704 a         1068 c          842 g         1861 t
ORIGIN      
        1 gtccatgact cctgcagcta acagtttgtc aaagatgtag ataacgattg tgaaaaatac
       61 agtgtattcc aagatggaaa taaaatcttt ccaacgttgt tttcgatttg gccaagtagt
      121 gtcacgcaat acttgaaaaa tccctttgac aaacattatc agttctcctt tttgcagttt
      181 gttacgctat atcaacgcgt ttctttgtgt attgtatatt tcttacaata tttacaaaat
      241 ttatttactt ctagtcgtgt tggcttgggg gtgctactta ctccgattga gtaatttcgg
      301 cttccgcatt ctgtacacgc taggcttgct ttcttttgtg ccataacgcc tccataagtg
      361 ttattttaac atgatttgta taatcctgca agctatctaa agtagtcctt tagactattc
      421 caagcatctt ttaccttctc ttggaaattt gaatcttcca cagctctaga aatatcttgt
      481 gcagccttac ttgccgaatc ttttaaatca ttaataattg agcgtgattc ttggttcgtt
      541 gtattactag catcttgtgc attataaact gaaccaatac cgttttggaa ataagcattc
      601 tcaatatgag taaaactaga ccccttagta tacggtagta tatatgaagc ttgcgtgcta
      661 aatatatttg aagctgtacc tgcactagaa tctgtcaaat aatggtgttt atctgtgttc
      721 ttaaagccaa cccattgact aataacgaca tcaggcgtat agccgactac ccactggtct
      781 ccagataaat tggggttaaa atctgtttcg gttgttccag ttttacctgc cattgtataa
      841 ccataaacat tggcattaat agctgtaccg tttgagaatg ttcctaacat catactagtc
      901 attttactag ccactgagcg actaataact cgcttaggct tatctgtaaa ttgctttact
      961 aattttccat tagctgtctc aattcgagta atcaaatgag cgcgatgcat tataccatca
     1021 tttgcaaaag ttgagtaagc ttgtgccatt tctaaaggat tagtagttac acttccacct
     1081 agcgccactc ctagctcttt gtttgctgat gacatattta gaccaaactt cctgccataa
     1141 gtaaaagctt tattgattcc tagtttatct aaagtataaa cagcaggaat attataagaa
     1201 ttagctagtg cttgatacat cgggatactt tcagtctcaa ttccaccata gttacttggt
     1261 ttataaccat gaaaatcttg aactttatta ggaagttctt tatctattga ccatcctgaa
     1321 gcaatagctg gtgaataaac aactaaaggt tttatggttg aggctggact tctctttgat
     1381 tgagtcgcgt agttaaaact tctaaactgt gcatctttag tactttgaac acgtccaact
     1441 aaacctctta caccaccagt ttttggatct agagctacgc tagcaccttg ggcagattga
     1501 ccatcatagt cagatacagg gaagaggcta gtatcatcaa atgttttttg cattcctgat
     1561 tgataatttt gatctaatgc agtgtaaatc ttatagccat tattgacaat atctttttct
     1621 gaaataccat aagtatcaat ggcttcattg acaacagcat caaaatagga tgggtacttg
     1681 taatcattaa ttttaccagc ataggtatct gctaatctat ttttcatacc aattgaagca
     1741 gcttctttag cttgagactt ggttagttga cctgcatcaa ccatcgctgc caaaacagta
     1801 tctcttcggt tagttgcatt ttcaacagaa taataaggat tataaacttc tggtccttta
     1861 agcattccag caagtgtagc agcctcatct actgtcaaat tagcagcact ggttccaaaa
     1921 tatttcctac tagcatcttc cacaccccaa acaccattac caaaataaga gttattaaga
     1981 tacatggtca gaatctcagc cttgctgtat ttcttagtca attctagcgc taaaaagaat
     2041 tcacgcgcct tgcgtttaat cgtttggtcc tgtgacaagt aagcattttt tgctaattgc
     2101 tgggtaatag ttgagcctcc gccaaatttc ccaagtgtag ctactgccaa aaaaaatcgc
     2161 ttaaagttta caccattatt ttcataaaaa gttctatctt cagttgctat aacagcattt
     2221 ttgagatgat cagaaatagc atttaactct acataagttc ccttttgtcc cgaaagtttt
     2281 ccagcgtatt ctttattctt atcataaatg gtggtggttg tttttagagc actctgtaaa
     2341 tcactaactg tagcagtttt agaaagataa aaaagatagc taccaaatat aagaataaaa
     2401 gccattaagg cgataaaaag caacttccca atacgatagc gccgccaaaa acgacggata
     2461 ggattcttag gagaagggag gtaacgatca actttttgta gccattcaag tcgagattta
     2521 gcattttcct tttggtgacg aatgcctcgc tgatactggg atttactagg tcgggtaata
     2581 tcatcaatat catagacttc ttcgccatcc aaattgctag tttcttctct agtttcttgt
     2641 tcattttttc ttgtcatttc gtcgttgtct tcatcaacac gaaataagtc tataaactta
     2701 tcaaataatt tcatagactt attatatcaa ttttcaataa aatgctataa taaaaccatg
     2761 tcattttcat taaaaattag aaatccatac ggtgaacata ccgttaaaga actccttgaa
     2821 gattattttt tgattccacg taagattaga cattttttgc gtgttaaaaa acatgtactt
     2881 ataaacaatg aattcattaa ttggcaaact gtcgtccaag aaaacgatac tattacctta
     2941 atctttgatg atgaggatta ccctactaaa aaaattcctc tgggcagagc agagcttatt
     3001 gattgtcttt atgaggatga acatcttatt atcgttaata aacctgaagg tatgaaaact
     3061 cacggtaacc aaccaaatga aatagcactg ttaaatcatg tatctgccta ttctggacaa
     3121 acatgctatg ttgttcatcg cctagatatg gagaccagtg gagctgtttt atttgctaaa
     3181 aatccattta tacttcccct tatcaatcaa cgcttagaac gaaaagaaat ttggcgtgaa
     3241 tattgggctt tagttgaagg aaaattttca cctaagcatc aagttttgag agacaaaatt
     3301 ggacggaacc gtcatgacag acgtaaacga atcattgatt ctaaaaacgg tcaacatgct
     3361 atgacaatca ttgacgtttt gaagtatatc caaaatagta gtctcataaa atgccgactg
     3421 gaaaccggaa gaacccatca aattcgcatt cacttatctc atcacggaca tcctttaata
     3481 ggagatcccc tctacaaccc ttcttctaat aatgaaaggt taatgctaca cgctcaccga
     3541 ttgactctat cccatccatt aacttgcgaa actattagcg tagaggcccc ttcatctact
     3601 ttcgagaagg ttttaaacaa ttataaaaaa ggagttggat aataattatc caactccttt
     3661 taatcattat tgcttagtta atggcttact gagtagtggt ttatggtaat caaattctcc
     3721 atcactagat gtctgccaga ttactgggat gatcttattc ccttcaattt ttattccata
     3781 ggcgtattta actttaagac caccgatacc tcctgttaca aaagttgtag tatcagtacc
     3841 tgattcatat tgataaaggt agagcccatt tcccttatcc tccaacttcg taactttagc
     3901 agaatggtta ctttccttag atttactatc cttgtaaact tttttctgag taactttacc
     3961 atccttatca aaagtcatcg ttactgtacc gtcctcaact tcacctttcc aacttcctac
     4021 aaggttctct ggaaaaatag aaacgggatc agccttaact ttattagttt ccttagctac
     4081 tgtgatatct tttttaccat cattagaaat aaggtaacta tttcccgatt ttttcataat
     4141 aaaattacca gttaaattct gaataatgtg gccatatgtt ttcttatcag gatctgttga
     4201 tttatcataa ttaaaatcat aatcggcagc aaaattaact tgataacttt ctttcccaac
     4261 ctgtgtcatt ttattaagga caatattagg aatagtgaaa tggctcgctt tacgattatc
     4321 agttacaaat tttgctttaa tactttcttt caatcctttg taaaaatcat tttcggcacc
     4381 tttttcaaaa actgttccta aagtctgagg gtcttgccca gttgatacat aaagtatcaa
     4441 ttgattgaaa gcagttatca atttctgtcc agcttctttt tcatttaata aaccatcaac
     4501 atctaatttg attgtctgat tatcagcgat agagattaat ttttgtttat gagaggttat
     4561 ctcaccatca ttaaaaactt ttttgatata agcttttgaa ccatcagtca ctggataatt
     4621 ctctacggaa tagctaccat cttttaattt agcaatacga ttatcaccaa aataaagatt
     4681 accatccata agattacttg ttactgtaaa agatttaaaa gcgaccgata aatcaatagt
     4741 ttggttttta ccatcataat cttttttgag tttaatctct tttccgtcac tagtcccttc
     4801 aaggctagca gtataatggg ttctaggtaa acgttccacc gttactgaaa aatgatctga
     4861 attgcttgtc gcgacctttt tttgattcaa caaaacatcc aatttaggaa cattggtttt
     4921 caaggtcaaa gacataggct tattagcaat acgataatca gggaatatac ctaagcgact
     4981 acctacacgc tttagataca ttgaatcatt tttgtttcct tcttttattt cctgccctaa
     5041 tttacgcgcc tcacttttag acaaaggctc aaaattagcg taattggcct catcatttgt
     5101 gatagattct cccgtatctg accaaaccat gtattcttta atattatcaa acgcttgacc
     5161 tgacgtacga gaacgtgctg ctacaaaacg actaagtact tgtccctttg aaaaataaaa
     5221 gaatcctaga ctcagaagtg atactaaaac tataccaaaa ataactaagc acactttaac
     5281 aacttttttc ttaaaaaaat caaacatacc ttatcctctc attcacttta tattaaagta
     5341 aattgcttaa agaaaagtta caacactgat aaaacagctg tatttgtact tgtaaccgct
     5401 atttttctat tataacaaaa cgaaaaaagg attgaaacca taatccgatt tcaacctcta
     5461 aaatttttgt ctatg
//
LOCUS       DATRSG010000090         5435 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRSG010000090 DATRSG010000000
VERSION     DATRSG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.091"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1019)
                     /locus_tag="VBD89_001871"
     CDS             complement(<1..1019)
                     /locus_tag="VBD89_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO7136369.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            1123..2250
                     /locus_tag="VBD89_001872"
     CDS             1123..2250
                     /locus_tag="VBD89_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO7136370.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIEIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            complement(2287..3273)
                     /locus_tag="VBD89_001873"
     CDS             complement(2287..3273)
                     /locus_tag="VBD89_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO7136371.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANI"
     gene            3436..3742
                     /locus_tag="VBD89_001874"
                     /pseudo
     CDS             3436..3742
                     /locus_tag="VBD89_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3900..5273)
                     /locus_tag="VBD89_001875"
     CDS             complement(3900..5273)
                     /locus_tag="VBD89_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7136372.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
BASE COUNT         1909 a         1100 c          794 g         1632 t
ORIGIN      
        1 tattttctaa aaatcgcttt agatatgata taggtcatta taattgacaa aagaattgtg
       61 gtagctaatg ctaacagttg aactggcata tgtgataatt taaagagacc aactcctaaa
      121 cttcccaaca atcccccaat accattcatt ccaaaggagg aaacgacatc atcaatggca
      181 aaagctttcg caacgtaatc cgttacaaag taggtgaaga gggatgcaaa gaatgttaca
      241 gctaataatt gccaagttaa gagataccca actcctgctg tactcgtcac aagaccaaca
      301 ataatgccat tcaataagct tcctgttttc tcttcctcac cattccactt agcagccaat
      361 gtccacgcca aaccaccacc tataatagca aatatagtat ttaaaagtat cataatggct
      421 tgtgaattcc actcaccaac tggacccata ttaaatccaa accaaccaaa tgtaataagt
      481 atcattccta aaaagagact atcattttta accttattgt gctgatgttc actcttgcca
      541 ataacatgag ctaaaatata cgaagaaagc cctgcagata aatgaacgac cattccgcct
      601 gaaaaatcaa gtactcccat ctttgcaaaa acaccctctg aattccaaat taaataagct
      661 aagggcgtat acaccaagaa aagccaagaa acaacagcta ataaaagtcg cttcgtctga
      721 actctatcaa taattgaccc aataagcatt actactgcgt aaagggcgaa acaaagttga
      781 aataaacaat ttacaatttg tttaccatct gcttcttgga attgaaagac agttttaaaa
      841 tgaccttcaa atgctaaaaa ataggcaccc atgaaccaag ctgttgtcgt tagcaaaaca
      901 gtaagtacaa actgataaat aatccttgaa gtcaatgatt gatgtagcga cccaaagtaa
      961 tagaatgcaa ccccaaaaat catgagccac atcgataaaa tacataatag taaaaacaca
     1021 aatagtcctt tctttacaaa cactctttta tttgttaaac tgtattgttg ttaactggtt
     1081 aagtatttta tcttacacat ttatttttgt caaggagaag acatgaaaca ttatcaaacc
     1141 ataattatcg gtgcaggcgc tgcaggaatc ggttttggtt ctgccatgca acgattagga
     1201 cttacaaatt ttcttatcat tgaaaaaggg cacattggag aatctttttt aagatggccc
     1261 agaacaacac aatttatcac accttccttc actaccaatg gctttggttt tccagatcta
     1321 aacgctgtta ttccagatac ttctcctgct ttctcatttg aaaaagaaca tctttctgga
     1381 gtggaatatg cacgctactt acaattagtt gctgctcatt ataatctccc tatccaaaac
     1441 gaaacatctg ttctatctat tgataaacga gatagccttt ttgttattaa aacttcaaag
     1501 ggagattttt ctgctgatta tcttattatg gcaacaggag aatttcaaaa tccaaatacc
     1561 attgagatta aaggtgcaga tctaggaatg cactatggtc aagttgataa tttccatatc
     1621 aaatctgaca atccatttat catcattggt ggaaacgaaa gtgcttgtga tgcactaaca
     1681 catctagtat atttgggaaa tcaagtcgaa ttatatacag atacgtttgg tcgtaaagag
     1741 agcaatcccg atccaagcat ctctttatcc cccctaacta aagaacgttt aaaacatata
     1801 caagaccaca aaaaagaata ttatagtatc tctgaaggca agaaagccat tgagattaaa
     1861 caaattggaa aacaatatca agttactttt gatgacggta gtactgcgga gtctttccat
     1921 aaacctattc tttccacagg ttttctaaat acctgtcatc tcattgacgg aatcgcttta
     1981 tttgaatacg ataaaaatca gttgccaata gttactgaag atgacgaatc tactatcgtt
     2041 aataactgtt tcttaattgg tccaagctta cgacaaagag atactatttt ctgctatatt
     2101 tataaattca gacagcgctt tgtccctgtt attaatcata tcgcacaacg agaagggatt
     2161 atattagacg aagataccct tgaattcttt aaagaaaatc aaatgtacct tgataaccta
     2221 gcttgttgcg atgtcaattg tgattgctaa aaaatcgcta gctttattgc tagcgatttt
     2281 ttaatattaa atatttgcta cttgaggtaa ggattctcca atagcttcta aacgttctgc
     2341 ccattcttga cgtgataaac cattttcttg cacatagcga ttacgttcat gtgcacgaca
     2401 ttcaggcgag caccctcgaa catacttcgc ttcgttttct tctgaagcaa aaatttgttt
     2461 gttacaaaac ggatttgcac aattgacata acgttcacaa ggtgttccat caaaataatc
     2521 tttactaata acagttggat taacatgatt aattggcacc gagatacgat catcaaaaac
     2581 atacatcgca ccatcccaaa gttcaccttg aacttccgga tcttttccat atgttgcaat
     2641 accaccgtgt aattgtccaa catctttaaa accttctcga accatccaac ccgagaattt
     2701 ctcacaacga acaccaccgg tacagtacac aacaacacgt ttttccataa acttatcttt
     2761 gttgtcacgt acccattgtg gtaactcacg gaaattgcga atatcaggac gaattgctcc
     2821 acggaagtga cctaagtcat actcataatc attacgcgta tctaaaacaa cagtatcttc
     2881 atcaagcaag gcttctttaa attgtttagg atttaaatac tcacctgttg tttccaaagg
     2941 attaatatcg ctatcaaagt tattatcttc taacccaagg tgcacaatct ctttcttata
     3001 acgaacaaac attttacgga aagcttgttg attttcctcg tcaatcttga accaaagatc
     3061 agcaaaacgc tcatcgctgt ggatccaatc catatacttc tgagttgttt cataatctcc
     3121 agaaacagtt ccattaatcc cctcatccgc aatcaaaata cgacctttaa gcccaataga
     3181 tttacaaaat tctagatgtt tcgcagcgta ctcttccgca ttttcaattg aaacatattt
     3241 ataatagagt aaaactctga ttttttctga cattttttct cctcatctgc aaaataagtt
     3301 agtatacttc attatataac gactccttac aacatagcaa aaaatacgct taggacatat
     3361 tctttgcttt aaagacaaat tattcttctt aagaacacac atatgttaaa atagtaaaag
     3421 actaggagga cttttatgac ttatgaatta tgtctagaat acggtactta tccacttaga
     3481 cctgtagatg cttgggctga tgaaatcaat actgcacctg cgtttattac tgaagataaa
     3541 aaaactttta gaacttttag aagaagtcaa taccttattc catgagctct ttttaacgat
     3601 agaatgttcc ttccactata ttggtcatga cttcccagaa aaaagagcaa aaatcacaca
     3661 aatttatcat gtgattatcg aacatttaag catacactac cctgaatatg acattaaaat
     3721 tgaatcatta ttaatggggt agttttattt taactaggta gaaactatct attaaaaaca
     3781 atcgatatca ctaattaatt caacaaatag attatctaaa acaaaaaagg tctctttcaa
     3841 gaggcctttt ttcataactt atctcactaa atattaaatg atacttttac caaataatat
     3901 caccaatact ctagactctt atctcttgaa agcaaggtac agacaatagc cgcaaatcca
     3961 gaaataccaa atattataaa tgctactccc caaccaaagt ggtcaataat aataccaata
     4021 gctagagtag cgatcgttgc tcctccgaca taactaaatg tgccagtcaa tcctgaagct
     4081 gctccagctg ctgcttttgg aactaactcc atagcataca ggccaaccat cataacagga
     4141 ccgtagattg atgcccccaa acctagtaaa ataatgaagt ctaatgccat atattgaggt
     4201 cctgctggaa taaaccaata ggcagctagt aataagattg ctacaactaa gaaggctaca
     4261 ttggttaaag gtttagagtt tttaaacact ttggcagata gatatcctgc tgttagcatc
     4321 ccaataatac cacctatttc aaaaagtgaa aaaccaccca taccagctgt tgcagtaata
     4381 ccttttccac catcttgcac actcgtcgct aaaaacttag gagtccatga gacaataccg
     4441 taacgtaaaa tatatacaaa tattgaatac aaggatgccc acatcagttt agtatttggt
     4501 agaatatatt tttttataat ttcgcccata cccaaaactt caggctcttc taaaatgtca
     4561 cttgaatcca caacattagt atattgctct ttatgatact cttctatagg cggaagtccg
     4621 atagattctg gagtatcttc ttgtaaaaag taaacaagaa cagccaaaag acaagccacc
     4681 acccctggaa accaataagc agcacgcgct tgattaagag aatcattacc agcaagagct
     4741 agacctacac cagttaaaat cggcgctatt gctccaccaa aattgtgaga taaattccaa
     4801 atggtagcat atttaattct ttctttatta tcataccaat agactaatgt acgcgctcca
     4861 ggagggtatc ccatcccttg taaccaacca ttaataagat acatgatagt aaagagccag
     4921 atactagtaa ttacagatgg aataacaccg attaacacat taactagtcc tgtaagaaac
     4981 aaaccaactg gaaggaaaat tttagaattc gattcatctg aaatcaaacc agaaataaat
     5041 ttagagagac cataagcaat gtagctagca caggtaataa taccaatctc agtagcagac
     5101 atattaagga taccagataa aattgaagtc gtattacgaa caaaatagta tcccatataa
     5161 cctacaaaaa tagaaataag gacttgtaca cgataacgag gatatttatg cttaatttcc
     5221 tcagctgata aacgttctct tttctgaggg ggtgcaaata gttttttcaa catagcaaat
     5281 tacctttccg atttttagat gtgtttaatt atacaactat gaattcacac tgtcaattaa
     5341 ttaataattt attttttatc attttatctt atttttttaa atgtggataa tactctttaa
     5401 agtgtagata atactatatt ataagctttt tatct
//
LOCUS       DATRSG010000091         5369 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRSG010000091 DATRSG010000000
VERSION     DATRSG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.092"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            377..595
                     /locus_tag="VBD89_001876"
                     /pseudo
     CDS             377..595
                     /locus_tag="VBD89_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1251..2990
                     /locus_tag="VBD89_001877"
     CDS             1251..2990
                     /locus_tag="VBD89_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7136373.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHRNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKKGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            3002..3736
                     /gene="ltdR"
                     /locus_tag="VBD89_001878"
     CDS             3002..3736
                     /gene="ltdR"
                     /locus_tag="VBD89_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO7136374.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            3892..>5369
                     /locus_tag="VBD89_001879"
     CDS             3892..>5369
                     /locus_tag="VBD89_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO7136375.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSL"
BASE COUNT         1753 a          879 c          946 g         1791 t
ORIGIN      
        1 tctggtgata ttgatttttt taatagataa tagaattcat attcagtttc tagtgccaaa
       61 acctatgcca ttaattgatg gaatgaccct ctgtaggatt taaagtactt tgtttaacta
      121 atgatgcgtg cgcatcatta ggaataggag tatcggcgat atcaatagtt ggtataacaa
      181 gcgaaattgc acaagtaaaa ttaaaaccag gaccagttga aataaaatta tccgttggtt
      241 tgtaggaagg attagttttg ggattattac taaaattgac tggaaactct gagagtttag
      301 ttcttcaaat atatgatatt gatacgaaga agtgggaaga aagaatcagt ctaaaaaagt
      361 agaagaaggt ttttttgtga aatcaaaaat agctaatctt gaatatcata caaataataa
      421 tattacttca aatgaaagag tattagcttg gtctgataaa gaacctacaa ataatatgaa
      481 tttaaaaatc ttgagtctta cattgatatg tataccgtaa ctatattgca ttgagttggt
      541 tgtttgcgat agcaacgtca agacagcaat aaaattgaaa aaatcaataa gataaaaagg
      601 atttaaaaaa ttgattatta ttgtggaagg gataagctta atgatgattt taacagcatg
      661 tgacttagta aaaaaagatc accaatgggt tcaagaacat tgaaaatact tagaagctgg
      721 atcaaaagtt atcaaagaag tcagccaaaa cattcaacca gttatttctg atctgaatag
      781 ctatctcaat ggagcgacaa gtgcttttga gcaatcggat tcgagtgcag ctactcatat
      841 taaaaataac accatcgccc aacttaaaaa aagcagtacc aaatcgtgtg agaaattacc
      901 aggctaattg tgagtgagaa tctattgttc tcactttttt attatcaaaa cgcttaaatt
      961 ctttacttag acctcgccta cacacttcag ataatgggtg ttaccatatt aattaataaa
     1021 tatgtaaaaa ataatatttg atatgctata ataataatat atttgattaa tcaaaatttt
     1081 ttagtgaaag tatctatcat ccttaagtag ttgttttcat aatagttgtt gatggatatc
     1141 cccttaattg tcttgagaat acttgataga gagacatttt aaatgagtaa tgaaatgaat
     1201 cattttatac ctatgttata acggactaat taatactttg gagagattta atgacactct
     1261 ttttaattat gatggagcga gcggggttaa tcatattatt agcctatgcc tttgtgcata
     1321 ttccctttat aaaacaaact ttgaagcaac ccgaattaaa aaaacaccag tatattctgc
     1381 ttatcttatt tagcttattt gctattattt ctaattttac aggggtagaa attcagtcag
     1441 atttatctat cattccacaa actcttaatc atatagcaga tcaatcatca gttgccaaca
     1501 cacgtgtttt aaccattggt gtatcaggtc taatcggtgg ccccatagtc ggtattatag
     1561 ttggtctctt atctgtattc gttaggtact tgcaaggagg attagcccct catatttatg
     1621 tcatttcttc cttactaatt ggtttatgct ctgggttatc aggcaattat cttcgtaaaa
     1681 attataataa aattcgtgtt ttagatgcaa tggttgttgg tttcgggatg gaaattctac
     1741 aaatgatctg tatcctcatt tttagtgttg actttaatca ggcgcttcga ttagtatctt
     1801 ttatcagcat gccaatgatt ttaagtaata ctttaggttt aggtatattt atatccatta
     1861 tttcatctac tcaaaaatta gaagaacatg ccaaagcatt tcaaacccat caagtacttg
     1921 aattagcaaa tttaaccctt ccttacctta gaaagggact gacaacagaa tcttgtcaac
     1981 cagtagctga aattattcat aaacatatgg atgtgagtgc tgttagcctg actagtcaat
     2041 cagccatttt agcctatgta ggagatggag ctgaccacca tcttcctaat actcaaatat
     2101 taacaaagct tgctaaaaga gcaatagata ctggtaaagt tagtgtcgca actgataaaa
     2161 gtgaaatcga atgcgaccat aggaattgcc cacttagttc agcaattgtt attccattac
     2221 acatccacga tgttatagtg ggtactttga aattatattt ttcagacgca caacatatga
     2281 cttacgttga ccgtcaatta gctgaaggat taggcaatat cttttccact cagttagctt
     2341 tgggacaagc tgaggaagca acaaggttac tacaggatgc ggaaatgaag tctctccaag
     2401 cgcaggttaa tccgcatttt ctttttaatg cactcaatac tatttatggt ttaattcgca
     2461 tggatagcga aaaagcaaga aagctcgtcc aagattttag caaggttatt cgtgctaatc
     2521 ttcaacgggc aaagcaaaat ttaattcctc tccatgatga attggaacag gttaacgcct
     2581 atttagccct tgaagaagca agatttccta acatggtggc ctttaattta gacaaccaaa
     2641 caaattcaga tgacaactta atgattcctc cctttacatt gcaggtcttg attgaaaact
     2701 cttataaaca cgcttttaag catatcaata aaaataacca gctaaaggtt actattgcaa
     2761 gaaataatga taggcttcat atcattgtcc aggacaatgg tattgggatt ccaaaagaaa
     2821 aattaataac gcttggaaaa aaaactcaaa tttcaaaaaa aggttctggt acagctattg
     2881 agaatctcgt tcgtcgcttg aatattattt atgatggaca agcaagttta aaatttgaat
     2941 caaatgattc aggaacttgt gctattgtta acatccccat taaaaattag aaaggtattg
     3001 tatgaaagtt ttagtagttg atgatgaacc agttgcacgt aacgaattaa tttaccttct
     3061 taataagtat gattctaacc tcgttatagc agaggcgcat gatatggcta ctgcattagc
     3121 tattttactt agagaaactt ttgatgtagc actgttagat atccatctca gagatgattc
     3181 tgggttgcaa ttagcagagt atatcaataa aatgcccaaa ccaccattat tgatattcgc
     3241 gactgcttat gatcaatatg ctattcaggc ttttgagcag gatgcgcgtg attatttgtt
     3301 aaaaccctat gagtttgata ggttaaagca agctatggat agagtaaaag gagcgctaag
     3361 tacatctaca attatagaga gcgtagcttc cggtcctctc ttcaagcaac agtatccatt
     3421 gacagtagaa gatcgaatct atctggtgtc ggcggatgat atccttttga ttgaagctat
     3481 gcaaggaaaa ctgattatac aaacacctga taaaaattat gaaattgatg gctctctaca
     3541 acaatggcaa gataaactac catcatctca atttgtacgg gtacatcgct cttacattgt
     3601 gaatattaat gctattaaaa cgattgaacc ttggtttaac caaacacttc agttacacct
     3661 ttgtaataaa ataacagttc ctgttagcag agcaaatgta aaacccctaa aacaaatgtt
     3721 aggcatatct acctaactaa tgaaaagtta ttcatcataa aacttagact attcatttct
     3781 aaaaaacaac cattcatctt gaaaatagga aatttcaatt tttcaatgct atactaaagt
     3841 catgaaaacg cttaataaaa ttaagcaaaa aaatttttag gaggcagtga tatggtaaca
     3901 tttttaggag gggtagccct tttaattgtt ggttatttca cgtacggtag atacattgaa
     3961 aagaattttc aaattgatga aaatcgccaa acacctgcag aagcattaag ggatggatat
     4021 gattttgttc ctatgccaaa atggaagaat gggatgatcg agttattgaa catcgcagga
     4081 actggaccta tctttggacc tattttagga gcactctatg gtccagttgc ttatatctgg
     4141 atagtcttag gatgtatttt tgcaggggct gttcatgatt atatgattgg aatgatttct
     4201 cttagaaata atggtgcata tcttcctgaa ttagctagtc gctatcttgg aaaatcaatg
     4261 aagcatgtga tcaacatttt ttcaatgcta ttacttattt tagttgcaac cgtatttgtg
     4321 gtaactccag ctaatcttat cctgagtatt ttaccagctg ggacactatc tttaccttgg
     4381 ataatcggtc ttattttcgt ttattacctt atttctacag tattacctat tgataaagca
     4441 cttggtaaag tttatcctgt attctgcgtc atcctaatgg ttagtacagc agcagttggc
     4501 ttccgtttat taactggtgg ctttgatatg ccaaacttaa cttttgaaac ctttaaaaat
     4561 atgcatccag ctggacttgg gatattccct gctttattct ttaccatttc atgtggagcc
     4621 atctctggtt tccatgctac ccaagcacct atggtctcaa gaacaactgt taatgagaga
     4681 gaagggcgtt tcactttcta tggtatgatg attgcagaag gagtaattgc gatgatttgg
     4741 gcaggtgctt caatgagctt gttcaaaggt caaaatctct atgaaatgat tgctgctgga
     4801 acaccttctg cagttgtcaa tcaagtaatg ttaatgttac tcggtagtgt aattggaact
     4861 atcgctatca ttggtgttat tgttctccct gtttcatctg gtttatcagc tttccgtagc
     4921 ttacgtacta tcgttgctga ttatatccat gtcaaacaag atactcttcc aaaaatcttt
     4981 gctgtaacta tcccattata tgttatttca tttgtcctta cacacgttga ttttaatctc
     5041 ctatggcgtt actttaactg ggcaaatcaa gtaactgctg ttattggttt attagttgct
     5101 acaagatatc tcatcctaaa aagacgaaat tattgggtaa catttgtacc agctatgttc
     5161 atgttgtatg cagtagttgt ttatatttta tctcaaccaa ttggatttaa tatgggctta
     5221 ggcatcttaa cttattcact tgctttagtg ttaacaggta ttcttgttgg acttttctgg
     5281 aaatctggtc aaaaacaatt aaaaactgtt catcctgaag cattcctgtt taacgatcat
     5341 agaccaatta attattacag tagtttaga
//
LOCUS       DATRSG010000092         5210 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRSG010000092 DATRSG010000000
VERSION     DATRSG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.093"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..192)
                     /locus_tag="VBD89_001880"
     CDS             complement(<1..192)
                     /locus_tag="VBD89_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136376.1"
                     /translation="MELIFRIFEMKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSR
                     RLVVKLLPDDYKFNLYIALL"
     gene            complement(255..1016)
                     /gene="recO"
                     /locus_tag="VBD89_001881"
     CDS             complement(255..1016)
                     /gene="recO"
                     /locus_tag="VBD89_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO7136377.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFYDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
     gene            complement(1006..2181)
                     /locus_tag="VBD89_001882"
     CDS             complement(1006..2181)
                     /locus_tag="VBD89_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7136378.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEASTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            complement(2289..3257)
                     /locus_tag="VBD89_001883"
     CDS             complement(2289..3257)
                     /locus_tag="VBD89_001883"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7136379.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            complement(3381..4682)
                     /locus_tag="VBD89_001884"
     CDS             complement(3381..4682)
                     /locus_tag="VBD89_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7136380.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAVAEAKAQAAAQVESATAPTETVQTQPRTEIKPSNLTATSSATT
                     VAKTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSNTYPMGQCT
                     WGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGYGHVAVVTS
                     VANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            complement(4918..5005)
                     /locus_tag="VBD89_001885"
     tRNA            complement(4918..5005)
                     /locus_tag="VBD89_001885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4969..4971),aa:Ser,seq:gct)
     gene            complement(5019..5092)
                     /locus_tag="VBD89_001886"
     tRNA            complement(5019..5092)
                     /locus_tag="VBD89_001886"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5056..5058),aa:Ile,seq:gat)
     gene            complement(5127..5197)
                     /locus_tag="VBD89_001887"
     tRNA            complement(5127..5197)
                     /locus_tag="VBD89_001887"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5163..5165),aa:Gly,seq:tcc)
BASE COUNT         1711 a         1036 c          822 g         1641 t
ORIGIN      
        1 taaaagcgca atgtataaat tgaatttata atcatcaggt agcaatttta ctacaaggcg
       61 tctacttaca atgatgccga taacttgaat tagaactgca ccaataactg gcacaaacca
      121 gttagcaaaa aagagtttat tggtaatgta taatgttttt ttcatctcaa atatcctaaa
      181 aattaattcc atattagttt atcctaatat taaaaaaaag aacatgacac taatgtcatg
      241 ttcctcttta caacttaatc tgatttcata atacttcccc aactagacaa atcatcaata
      301 aattttttac ttttaagatg aatccctaca tattcatcgt aaatcatgtc caaaaattga
      361 cgaattttaa gtttcatctc aggtttcaca gaaattgttt gtaaatcatc aaattgaatt
      421 gactgaaaac gattgattaa gtacagcata ttaggatcta ggtggtttct tctctcgtct
      481 ttataataat ggtttggaca taataatcca gaatatttgt gtgaaaaatc aaaaggtaat
      541 cctgccctat gacaaaaaac acaatcataa aaatttaaag caacaccgaa cctctctagt
      601 agttgtactt caaaaatatt cgttagaatc tcataatcta aaccgtcctc aatcaattct
      661 aacgttttct ttaggaaaat aaataattga gcatccgcta cattatcact aatagccaca
      721 tcagccaaag aagtaatata actcgcatat gacaacttaa acaagtctga attggtttct
      781 tgaaatgcta aaacttcttt ataatcatca atataagaaa gaccattatc attaattttt
      841 aaaataaaat gagcaatagt caaaggttgg agcacagcat tgaattttga ctttgaagca
      901 tgctttacaa aaaacattct tttcccctca gtctccgtaa agattttaac taatttatcg
      961 tcttcacgat aattacgatt atacaaaacg agaccgtatg tttgactaac cctcatatga
     1021 ttccataaaa actttcaatc tagccattgc tgtttcaata acttccatac tagcagcata
     1081 agaaagacgt atataccctt caccatattt accaaaagcc acccctggaa taatagctac
     1141 agcttgttga taagcaaaat cctgacaaaa cttgaaagaa tcctgtggat aactatcagg
     1201 tattttagca aagatataaa aagcgccgct cggtttaatt attttgaaac caagtttaga
     1261 cattttatca atgatataat ctctacgttt aatatatcct tgacgcatag gtaacgtatc
     1321 atttttaccc acagataagg cttctacccc agcaaattgc gaaatagtac tagcagctgt
     1381 caccatgtat tgatgacttt taatgatttg agcagtagat gcttcaggag catacaccaa
     1441 tccaactctc caaccagtca tagcatgtga ttttgataag ccattaatta agatagtttg
     1501 gttaggtaaa tattctgcaa ttgaaacatg ttgctgacca gtgtaagtta actcagagta
     1561 aacttcatca ctaataacaa aaatatcata ttttttaagg acttcagcta atgcagcaat
     1621 ttcttgcctt gaataggtaa tacctgtagg attagtagga taattaagta aaacagcctt
     1681 taatttttca ccttgttgga taatagccgt ttctaacatt tcaggggtta gtctaaagtc
     1741 attttctcta gtatcaattt ctacgatatc agctcctaca aggttaacta ttggctcata
     1801 gcctggataa gcgggggcag gtaatagaac aacatcacca gcttctaaaa tagctataag
     1861 agatgcagac agagcttctg tggcacctat cgtaaccaag atctcactat cagggttata
     1921 agtaagatta tatttatcct ttgcaaaatc tgcagcagct tgccttagag ctaataaacc
     1981 gctcatacca gtataatacg attgattcgc atcaatagct gatttagctg cttctttaac
     2041 atgatcagga gtggtaaaat ctggttctcc aagagttaat tttaccattc caggtatatc
     2101 tgaaatagat tgatcaaact gacgaattaa ggagacctct attctatcta agtacttatt
     2161 aaatcgtttc tctaatgtca tcataacctc ctgataagaa ataatatatt taattataca
     2221 agaaaaataa tttgtgtcca gttttccgtc attaaatgat aaaaaacctg ttaataacag
     2281 gttttttatc attttagttt attcatttca aataatggtg ataaaggacg tttctcgtga
     2341 atacggataa tagcttcacc aatcaattcc gcaattgaaa tttgttcaat cttatcaatc
     2401 aaacgttctt cagggaggta aatcgtatct aacacaatta atttttcaat agctgaattt
     2461 tgaatattat ccaaagccgg ccctgataaa acagggtgag tacatgaagc ataaacagct
     2521 gttgcccctg cttcagctaa agcgtctgca gcatggcaaa tagtacctgc agtatcaatc
     2581 atatcatcaa ttaaaataca ttttttacct ttgatattac caataatatt cattacttca
     2641 gatgtattca ttttagtaac actacggcgc ttatcaatga tagcaatcgg tgtctttaaa
     2701 cactgtgcca gcttacgtgc acgtgttacg ccaccatgat caggactaac gacaacaaca
     2761 tcatcaccaa ctaatccctg acgatcaaag taatctgcaa taaggggagc ccccataagg
     2821 tgatcaacgg gaatatcaaa gaatccctga atttgagctg catgtaaatc aactgttaaa
     2881 agacgatcaa caccagcaac ttctagcatg ttcgcaacga gtttcgaagt aataggttca
     2941 cgagaacgtg ccttgcgatc ttgacgagca tacccatagt aaggcattac cacactaaca
     3001 gactccgcac tagcacgctt taaagcatca accataatta aaatttccat caaattatca
     3061 ttaactggtg agcttgttga ttgtaaaata aaaacatgat ggccacgaat agactcttca
     3121 atattaactt gaatctcacc atcagaaaat tggcgaactg tcgattgacc tagcggaatt
     3181 ccaatcgtct gagacacttt tttcgctagt tctttgtttg atgacagtgc aaacaatttt
     3241 aaattagaat aagacatgat ttcctccaaa actttagtcc tataccattt taacgctttt
     3301 tacacaattt ttcaatccaa aataaaccat acctaattaa taaacaaaaa ggagaccacg
     3361 aaagatctcc ttagtattca ttaatttggg taaatatagt aaacagagcc agaagcacta
     3421 gggttaaatg aaccacggta attgccaatg ctcatatttc cagcatagtt tgattccata
     3481 acttggatag atgaattgtt agccactgac gttactactg caacgtgccc gtaaccacca
     3541 ccatcatatg gccaaacagc tactgcacca acacgagggg tagttcctac agaataacca
     3601 gctgcacgtg cactagcacc ccactgatta gcatttcccc aatagttacc aacccaagaa
     3661 gccattgact tagctcccca agtacattgt cccattgggt atgtgttact tgaatcataa
     3721 cttctgacaa caggttttga taccgctcta ggtgttgaag aaacaactgc tttaggtgct
     3781 tcaactgctt tagttactac agaaggttga gtaacttttg gctcattagt agctgtagct
     3841 gtagttttag caacagtagt tgccgaagaa gtagccgtaa gattagaagg ctttatttca
     3901 gttctcggtt gtgtttgaac tgtttcagta ggagctgtgg cagactcaac ttgtgctgct
     3961 gcctgtgctt tcgcttcagc tactgcttgt gcttcagctg ccttacgcgc agcttcctca
     4021 gcttgtgctt ttgcctgaat taaagatgct ttttcatttt ggactgtagt caattgagca
     4081 gataactcta attgagctgc ttctaattgt gctctttgtg ttgctaaggc tgctttatta
     4141 ttttcgatag cttgcttgtt agctgctaca gtatttatag cattttgatt ttcaatttgt
     4201 ttagcttcta aggctgcttt gtcagcctct tgttgtgcta acatcttttc attagctgaa
     4261 acaacctcac gaatagcaac aacacgatta acagcatcag atactgattt cgaatttaaa
     4321 atagtattaa tatagttagt caagttaccc ttttgagcac tacgaacttg ctttttcaaa
     4381 gattcgttac gtgcaacaat tttattagaa agagtttgaa tctcttgacc taactgctga
     4441 gatactgcct caagttgagc attttgcgct tctaattctg attgttgaga ctctaaagca
     4501 ccaacttgtc ctttaatagc agtcacctgg ttttgtgctg ctgcttgttg ccctgataaa
     4561 gtattgatta ctgaatcagt agcagcaatt ttcgagtcaa agtcatcagc gtttactgtt
     4621 acggcagcag ttccaagagt tactccactc acaaggactg ctgataatat tctttttttc
     4681 atttttatta aactactcct ttacgataag acattaaata ttttaccaaa aaattcacga
     4741 aattatatta cgtcattgtt acatttatat ttgaaatcaa ctatttctaa atgaaccata
     4801 atcaaatcta gaaaacgata accttcttct attcactcta tcaatataat tactccatag
     4861 tgaaactaaa agagaaataa aaaaagagta taattactct taaaattata atatttacgg
     4921 agaataaggg attcgaaccc ttgcgccagt tacccgacct aacgatttag caaaccgtcc
     4981 tcttcagcct cttgagtaat tctccaaatt aatattaatg ggcacgagtg gactcgaacc
     5041 accgacctca cgcttatcag gcgtgcgctc taaccacctg agctacgcgc ccaagcaaat
     5101 gcttggtttt acttttatgt aaagtaagcg ggtgacgaga atcgaactcg cgacaacagc
     5161 ttggaaggct gtagttttac cactaaacta cacccgctaa aaacttatat
//
LOCUS       DATRSG010000093         4982 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRSG010000093 DATRSG010000000
VERSION     DATRSG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.094"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            157..1881
                     /locus_tag="VBD89_001888"
     CDS             157..1881
                     /locus_tag="VBD89_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEO7136381.1"
                     /translation="MNNNEKKVKYFLRKTAYGLASMSAAFIVCSGIVNTPTVSADSPD
                     TLKVEKLGKLKDVKSVHELTPISIPNELKGAKEQALSSIISHPNITNSEVDKLASDYS
                     FRINTSNDVNDVKRLLNEFYNAVARKQLDTNSADYRSKIDNISTTGLAIALEAKEIYE
                     ANKSILPHRYKDSVGTYVNSFEERRSPGKFNIWNGQEGFNAAQKLLEDVKKLLLELQN
                     LTKNNKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDILSV
                     QKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPVAKGTYDVKYVD
                     TEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFTAGAQVRTYVYEKVA
                     KPVAKGTYDVKYVDTEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFT
                     AGAQVRTYVYEKVKPEVKPDVKPEAKPEAKPEVKPDVKPEAKPEAKPEVKSDVKPEAK
                     PEAKPATKKSVNTSGNLVAKKAIENKKYSKKLPSTGEAASPLLAIVSLIVMLSAGLIT
                     IVLKHKKN"
     gene            2087..2626
                     /gene="nusG"
                     /locus_tag="VBD89_001889"
     CDS             2087..2626
                     /gene="nusG"
                     /locus_tag="VBD89_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO7136382.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(2673..3878)
                     /locus_tag="VBD89_001890"
     CDS             complement(2673..3878)
                     /locus_tag="VBD89_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO7136383.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWFRLFLADYIPSSRVLYLDSDI
                     IVNTNLDYLFELDFKGHYLAAVKDPHKNEEGGFNAGMLLANLELWREDGLTKTLLKTA
                     EELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNI
                     IHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKVISYEKSMELLSVSDIHLF
                     TLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQYPNVLVYPELIEARIEKL
                     ITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQENITKHIVNHNSIDNMVVL
                     INELNLNQL"
     gene            complement(3943..>4982)
                     /locus_tag="VBD89_001891"
     CDS             complement(3943..>4982)
                     /locus_tag="VBD89_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO7136384.1"
                     /translation="VGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMTYARYFIPQYI
                     SADTVLYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGVMVINNQRWRQ
                     ENMTIKLIEKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFDMGAAIDGHKF
                     IFDIPITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISSKKVFGLDHPI
                     KTQNYRLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISLLAFENIRLHK
                     TYTPPIIDKLLAEADIYLDINVFGKMEGILQKMSQSEKPILTTRECNSQLVPETVFAS
                     IDDLINYLQSI"
BASE COUNT         1783 a          757 c          905 g         1537 t
ORIGIN      
        1 attcaattta ataaaatgta aataaattaa agaaaaagtg ttgaaataaa aaactccaag
       61 ttatataatt agttaaggaa ctcaaattaa attaaaataa acgtggtcct atcctaataa
      121 attaggattt ttaactaact atataaggga gcgaatatga ataataacga aaaaaaagta
      181 aaatactttt taagaaaaac agcttatggt ttggcttcaa tgtcagcggc gtttatagta
      241 tgtagtggta ttgtaaatac tcctacagtg tctgctgata gtcctgatac attaaaagtc
      301 gaaaaattag gcaaattgaa agatgtgaaa tcagttcatg aactcacacc catatcaata
      361 ccgaacgaat taaaaggtgc taaagagcaa gcactttctt caataatttc acatcctaat
      421 ataactaatt cggaagtaga caaactagct agtgactata gttttagaat taatacatct
      481 aatgatgtga acgacgttaa acgtctatta aatgaatttt ataacgcagt tgcaaggaaa
      541 cagttagata caaattctgc tgactaccgt agtaaaattg ataatatcag tactacaggt
      601 cttgcgatag ctcttgaggc taaagaaatt tatgaagcaa ataaatctat attacctcat
      661 cgttacaaag attctgttgg aacttatgtg aacagttttg aggaaagacg aagtccagga
      721 aaatttaata tttggaatgg tcaggaagga tttaatgcag ctcaaaaatt gttagaagat
      781 gttaaaaaat tattacttga gctacaaaat ttaacaaaaa ataacaaacc aaatattcaa
      841 gtacctaaac aagcacctac agaagctgca aaaccagctt tgtcaccaga agccttgaca
      901 agattgacta catggtataa tcaagctaaa gatctgctta aagatgatca agtaaaggac
      961 aaatacgtag atatactttc agttcaaaaa gctgttgacc aagcttatga tcatgtggaa
     1021 gagggaaaat ttattaccac tgatcaagca aatcaattag ctaacaagct acgtgatgct
     1081 ttacaaagtt tagaattaaa agataaaaaa gtagccaaac cagtagctaa aggtacatac
     1141 gatgttaagt atgtagacac agaaggaaaa gaagtagcta agtcacgtca cttcgaagga
     1201 gaagaaggcg cagcttttgt cacttcagcg aaagaagtag cgggttacaa acttgttaga
     1261 acggaaggcg ctgtttcaaa tgtcttcaca gcaggagcac aagtacgtac atatgtttac
     1321 gaaaaagtag ccaaaccagt agctaaaggt acatacgatg ttaagtatgt agacacagaa
     1381 ggaaaagaag tagctaagtc gcgtcacttc gaaggagaag aaggcgcagc ttttgtcact
     1441 tcagcgaaag aagtagcggg ttacaaactt gttagaacgg aaggtgctgt ttcaaatgtc
     1501 ttcacagcag gagcacaagt acgtacatat gtttacgaaa aagttaaacc agaagttaaa
     1561 ccagacgtta agccagaggc caaaccagag gctaagccag aagttaaacc agacgttaag
     1621 ccagaggcca aaccagaggc taagccagaa gttaaatcag acgttaagcc agaggctaaa
     1681 ccagaagcca agccagcaac caaaaaatcg gttaatacta gcggaaactt ggtggctaaa
     1741 aaagctattg aaaacaaaaa gtatagtaaa aaattaccat caacgggtga agccgcaagt
     1801 ccactcttag caattgtatc actaattgtt atgttaagtg caggtcttat tacgatagtt
     1861 ttaaagcata aaaaaaatta aatttatttt taaacataaa tcgaaaggtc tatcctttca
     1921 aaatcttaag gcaaacctca tcagaacttt gcctttttta tttgaatttt agaatgattt
     1981 agtcggtact ttaattgaca gcttatttta ttttctttat aatatatatt gaaaatagca
     2041 gtccaataag ccgtaaaagg cttttttatt aaggaaagga aaacttatgt tagattcttt
     2101 tgataagggt tggtttgtac tccaaaccta ctcaggttat gaaaataaag taaaggaaaa
     2161 tctcttgcaa cgtgcgcaaa cttataatat gttagacaac attttgcgtg tggaaatccc
     2221 tactcaaact gtaaatgttg agaaaaatgg gaaaacaaaa gaaattgaag aaaatcgctt
     2281 cccaggttat gtacttgtgg aaatggtaat gactgatgaa gcctggtttg ttgttcgtaa
     2341 tactccaaac gttactggat ttgttggatc acacggcaat cgctctaaac caacaccgtt
     2401 gttagaagaa gagattcgtt caatactcat ctcaatggga caaaccgttg atgtctttga
     2461 tactaatatc aaggaaggtg atgttgttca aatcattgat ggtgctttca tcggacaaga
     2521 aggacgtgta gttgagattg agaataacaa agttaaatta atgatcaaca tgttcggctc
     2581 agaaacacaa gcagaacttg agttgtacca agttgcagaa ttataaaact tctgaaaaat
     2641 ccatcttaaa ataaagatgg atttttgtta atttataatt gatttaggtt taattcgtta
     2701 attaaaacaa ccatattatc aatagaatta tgattaacga tatgtttggt tatattctct
     2761 tgatgagacg tgttgttgaa agaataaatt ggcttttgtt tcaaatgaac tctttgtaaa
     2821 atattaccta cttctggacc atgattaata tcaaggtatg cagtggctaa tgttattaat
     2881 ttttcaattc ttgcctcaat taattcaggg taaactagga catttggata ttgttctagt
     2941 ttgctaagtt tatttgacac tgttgtgtag gcaccaatat ggaattgaac agtaggacat
     3001 ctacaaatta gcgattcaat gtgttccaaa ttatcggaag atgtaagggt gaaaagatga
     3061 atatctgaaa cagaaagcaa ttccatggac ttttcgtaag aaatcacttt tctttgcttg
     3121 ccggttaatt ggcaaaaatc aagttggaag taataccacc atagttctct aaaccttgca
     3181 acgctatttt cattccaagg tttgtcacta gttaaaaaat gtataatatt aggagttcta
     3241 ttttctatgt ttagatataa ataagatcga tgattatagc ggctaacgac atcataagtt
     3301 tgaaagttcc atgttttgtt cagagataac caacgattat ggcaaacaat gttcaagata
     3361 ctttgatccc ctgttttgac aactcggtgg agttcttcag ctgtttttag taatgtttta
     3421 gtgaggccat cttcccgcca tagttctaga ttagctaaaa gcatgccagc attaaaccct
     3481 ccttcttcat ttttatgggg atctttgacg gctgctaagt aatgaccttt aaaatctagt
     3541 tcaaataagt aatcaagatt agtgttaacg ataatatctg aatctaaata aagtacacgt
     3601 gaactaggta tataatctgc taaaaataat ctaaaccatg tactaacagt tagaaaagtc
     3661 gttagatgag caatatcata gttatatatt ttaacattaa tcagttttga tcctagaggc
     3721 tctagtttcc tgtttatatt acgtacccat tcaactgggg tatcttgatt gaataaataa
     3781 aaagttatat ttctattata aacacaaata gactttattg ttactaaggc tttatccaag
     3841 tagtttgaat caactgcaag tgcaactgct ttttccattt aaattctcct agttaagtgt
     3901 ttagaataat gttgggtata tagtgaatgg atgccaattt ttttatatag actgaagata
     3961 attaattaaa tcatcgatag atgcaaaaac tgtttctgga acaagctggc tattgcattc
     4021 tcttgtagta agaattggtt tttcagattg tgacattttc tgtagaatgc cctccatttt
     4081 tccaaaaaca ttaatatcaa gataaatatc agcttctgct aataatttat caataatagg
     4141 tggagtgtaa gttttatgaa ggcggatatt ttcaaaagct aataggctta taagaatgtc
     4201 agacataact gttggtgctg caatgtgaaa tagacaatct ggaagggcag tgactaattc
     4261 tgagatagat ggtatacaat cagaagttgt aacaataagg aaattgagac gataattttg
     4321 tgttttaatt gggtggtcta aaccaaatac ttttttacta gatatgatac ttgaccattc
     4381 aagtaaatta tagtgccacc atacctcacg gagtctcata tttgataatg tttgccaagg
     4441 tttgattccc gaaatgtagt gaataatttt tgggagtggg gtaattggga tgtcaaaaat
     4501 aaatttatgc ccatcgatag cagctcccat atcaaaacca atttgaaaat tgtaggtatc
     4561 atctaaataa attacctgat tttcaaacat gcgattaaga attgtttgat ccccttcgtt
     4621 ggcattctca atttcctttt gatttttttc aattaattta atagtcatat tttcttgtcg
     4681 ccaacgttgg ttgttaatta ccattactcc agcattaaac ccatatccaa gcccaaaaag
     4741 agctggaact gcagctaaat atgcattgtc tagtgaaatt tgaaagaggt tatctaaatt
     4801 agtagtaaca actaagtcag agtcaagata taaaactgta tcagctgaga tgtactgagg
     4861 tatgaaataa cgagcatagg tcatataatt aatatgatta agctttttat tttcccattt
     4921 tggggataag tgatcatgga agagctttac atcctgaatt tgatttccag tttgtccaac
     4981 cc
//
LOCUS       DATRSG010000094         4728 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRSG010000094 DATRSG010000000
VERSION     DATRSG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.096"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            1..1122
                     /gene="dnaN"
                     /locus_tag="VBD89_001892"
     CDS             1..1122
                     /gene="dnaN"
                     /locus_tag="VBD89_001892"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO7136385.1"
                     /translation="MNKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAIILTGSN
                     GQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQHQIVLT
                     SGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRPILTGV
                     HLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDDIETVE
                     VFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAMERAYLI
                     SNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYLIESLK
                     AVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            1180..2073
                     /locus_tag="VBD89_001893"
     CDS             1180..2073
                     /locus_tag="VBD89_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO7136386.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGKIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYR
                     LGKITYIVIAIKSLLHSSKVQVLVEGETGQRIKLNDLYFFALANNTYFGGGITIWPKA
                     SALTAELDMVYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEI
                     DGEIVELDQISLKCQKRYLYM"
     gene            2083..2280
                     /locus_tag="VBD89_001894"
     CDS             2083..2280
                     /locus_tag="VBD89_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO7136387.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            2886..3359
                     /gene="dfrF"
                     /locus_tag="VBD89_001895"
     CDS             2886..3359
                     /gene="dfrF"
                     /locus_tag="VBD89_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HEO7136388.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            3466..3996
                     /locus_tag="VBD89_001896"
     CDS             3466..3996
                     /locus_tag="VBD89_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7136389.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            4542..>4728
                     /locus_tag="VBD89_001897"
     CDS             4542..>4728
                     /locus_tag="VBD89_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HEO7136390.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNC"
BASE COUNT         1680 a          666 c          899 g         1483 t
ORIGIN      
        1 ataaataaaa atttcttctt gcatgctcta acagtaacca aacgagctat tagtcataaa
       61 aatgcgattc caatcctttc aactgttaaa atagaagtga ctagagatgc tatcatttta
      121 acggggtcaa atggacaaat ttcaattgaa aatactattc ctgcttcaaa tgaaaatgca
      181 ggtttactag taacgaatcc aggctctatt ttgttagaag ctggtttctt tattaatatt
      241 atttcaagtt taccagatgt aactttagaa tttacagaga ttgaacaaca tcaaattgtt
      301 cttactagtg gaaaatcaga gattactttg aaaggtaagg atgtcgatca ataccctcgt
      361 ctgcaggaaa tgacaacaga tactccatta acattagaaa ctaaactgtt aaaatcaatt
      421 attaatgaaa ctgcttttgc tgctagccaa caagaaagcc gtccaatctt aacaggtgtt
      481 catttggtta tcagtcaaaa taaatacttt aaggctgttg cgacagattc acaccgtatg
      541 agtcaacgca ctttccaatt agagaaatcg gctaataatt ttgatttggt tgttccaagt
      601 aaatcccttc gagaattttc ggctgttttt acagatgata ttgaaactgt agaggttttc
      661 ttctcagata gtcaaatgtt atttagaagt gaaaatatca gcttctatac acgtttgctt
      721 gaaggaaact accctgatac tgatcgcctc ctaactaatc agtttgaaac cgaaattatc
      781 tttaatacaa atgctttacg ccatgctatg gaacgtgctt atttaatttc gaatgcaact
      841 cagaacggta ctgttcgttt agaaattcaa aatgaaacag tctcagctca tgtaaactct
      901 ccagaagttg gtaaagttaa tgaggaattg gatactgtta gccttaaagg tgatagttta
      961 aatattagtt ttaatccaac ttacctaatt gaatctttaa aagcagtaaa aagcgaaaca
     1021 gttacgattc gatttatttc tccagtacgt ccatttactt tgacccctgg tgaagatact
     1081 gaagatttca tccaattaat aactcctgtt cgtactaact aaataagaaa actacccaag
     1141 ttattcttgg gtagttttct tattctaaga tatgctataa tgatgagtaa tatgactcta
     1201 tatataatag ctaaccccca tgctggtaat aaaaatgcct ccactattgt tggtaaaatt
     1261 caggagcttt atcatactga agatatttct gtgttctata cagaacagaa agatgatgaa
     1321 aaaaaacaag tcattaatat actaaggtct tttaaagaaa gtgatcatct aatgattata
     1381 ggaggagatg gtaccttatc aaaagtaatg acttatctcc cccaacatat tccgtgcgct
     1441 tattatcctg ttggttcagg aaatgatttt gccagggctt tgaaaattcc taacctcaaa
     1501 gaaaccctta ccgctattca gacagaaaga ttaaaggaaa ttaattgctt tatttatgat
     1561 aaagggctga ttttgaatag tttagattta ggttttgcag cctatgtcgt ttggaaagca
     1621 tctaactcta aaataaaaaa tattttgaat agatatcgtc taggaaaaat aacttacatt
     1681 gttatagcta tcaaatcatt attacattct tctaaagtac aagtattagt ggaaggagag
     1741 acaggacagc ggataaaact aaatgacctt tattttttcg cattagcaaa taacacttat
     1801 tttggaggtg gcattactat ttggcctaaa gcaagtgctt taactgcaga gttagatatg
     1861 gtttacgcta aagggcatac ttttctaaaa cgattaagta ttttattatc tttggttttt
     1921 aaaaggcata ctacctctaa aagtataaaa caccagactt tcaaagctat gaccgtatat
     1981 tttcctaaaa atagtttgat tgaaatagat ggcgaaatag ttgagttaga tcagattagt
     2041 ttaaaatgtc aaaaaaggta tttatatatg taaggagaaa agatgtatca agttggatca
     2101 cttattgaaa tgaaaaaacc tcatgcttgt gtaattaaag aaactggtaa aaaggctaat
     2161 caatggaagg tactgagagt aggagctgat attaaaattc agtgtactaa ctgtcagcat
     2221 gtgattatga tgagtcgtta tgattttgag cgaaaattaa aaaaagtact gcaaccatga
     2281 aagtcctttt aaacaaataa ttatagctat atttgcaacc aatactttcc taaataattg
     2341 ctagtatgcc gttggaaaat tagctattct aatgttatcg aaagaagaaa ggtggccatt
     2401 gacaatgacg aaagttgcag aacagttgtt tatttttcgg taagtcatat caaaatggga
     2461 aaatggtgtt tcccaaacaa aaagatatta agaaacagcc catgtttacc tgcttgcaaa
     2521 caaaagaata ttgctactag actaagagat actacataag tgtgttggta tctctttttt
     2581 gttgccgtga aacagcagac tatttcattt ctgcttgatt accttaaaac ttattagtct
     2641 atggtttgtc aagggcttgt tgcgtgagaa atgctgaata tcacccttta caaaacatag
     2701 gctgataagt aacttttcaa aagcagaaaa gaaatggcaa tgcttataac aatatgatat
     2761 aatgcagatg aagacaaaaa aggaatttgt cgagctggtt agttcgagat aattttgagt
     2821 ttgttaagat gtggtgcact tgatggaatc acaggtacga catcatatta tggaggatga
     2881 aatagatgat aggtttgatt gttgcgaggt caaagaataa tgttataggc aagaatggta
     2941 atataccatg gaaaataaag ggagaacaaa agcaatttag agagttaaca acgggtaatg
     3001 tggttattat ggggcgaaag tcttatgaag aaatcggtca tccgttgcct aatagaatga
     3061 atattgttgt ttccaccaca acagagtatc aaggagataa tttagtttca gttaaatcat
     3121 tagaagatgc attattattg gctaaaggac gagatgtata catatctggt ggatatggac
     3181 tatttaagga agctttgcaa atagtagata aaatgtatat cacagaagta gatttaaata
     3241 ttgaagatgg agatacattc tttccagaat ttgatatcaa tgattttgaa gttttgatag
     3301 gggaaacact tggtgaggaa gtgaaatata cgagaacatt ttatgtaagg aaaaaatgaa
     3361 ttgagtaaat tttggattta gggtgttttc ataaatatat tgctttttat gcatatataa
     3421 attgttgtaa gacaaattcc tgtttgtatt tataaaagag aaaaaatgag aaaagtagtt
     3481 ttatttattg ccatgagcct tgacggatat attgcggacg gtaatggtgg agtagcttgg
     3541 ctaaatggtc atggaaatga caatgaaaac atcgacactt atacagaatt taccaaagat
     3601 atagatactg tcttaatggg gtggaatact tctcatcagg ttgttactga actttctccg
     3661 caagaatggg tatataataa atttacaact tatgtattaa cacataaaga gtgcaattct
     3721 tcccaacaaa ttcattttac aagtgaaaat cctgttttat tattggaacg gctaaaacaa
     3781 gaagcaggaa aggatatttg gatttgtggc ggagcaaatc ttgttcagca gttggtaagc
     3841 aaaaatatga ttgataaata ttacatttct gttattccta ctctgttagg aaatggggtt
     3901 cgtcttttgg gcaacataga cagggaaata aagttaaggc tttgtaaaac acagacttat
     3961 aacggaataa ccgatttgat ttatatgcgt agataactag agagaaacag aaaagctgtt
     4021 aaaccgacgg agcgatccga ccacgttggc gggaaaatcc ttaagatttt tccgccatgc
     4081 ttgcaagggg gcggttcgct tcggcggttc ggcgagcttg cgagccgttc aaagcccccg
     4141 ttatctaaca aggggggcac aaaaaacaag aaacactata cactaacaag cggaaaacgc
     4201 tgtatttata aggcaaaacc ccatttttac agtagtgata taaattgtac ctatatcact
     4261 atgaaaattg aatagtaaat atgagaaaga cagttgattt caaaaaataa gaaattgatt
     4321 gtcgttttct tttgcagaaa tttaagtgca ggcaattatg gtagttgtcg taattgctgt
     4381 cagctcagtg tttatcactg atggtgtgaa agatttacgg ttggagatgt tcaataagat
     4441 tattggtgct taattatttt tattgtagtg ctgatatgag aatgttataa ttggggtata
     4501 aaacatacaa ataaatttgc tgaaaaatga ggtatcaaaa tatggaaaat gaattactgg
     4561 aaaacttagg aagacttcac acaactgaac ttggcgttat tagaattaaa aagaacttat
     4621 ctttgaatgt agaaaatgta attgaatggt gcaaagaaaa aattagcctt tctaatgcgg
     4681 aaatcacaag aaaaggaaaa aactggtata taacgattga taattgta
//
LOCUS       DATRSG010000095         4661 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRSG010000095 DATRSG010000000
VERSION     DATRSG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.097"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..506
                     /locus_tag="VBD89_001898"
     CDS             <1..506
                     /locus_tag="VBD89_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136391.1"
                     /translation="YLRKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQE
                     FQKFKKKVLNNFKYIKPAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMA
                     NMIILERANGLRELFFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWS
                     ITGIKTK"
     gene            1160..4600
                     /locus_tag="VBD89_001899"
     CDS             1160..4600
                     /locus_tag="VBD89_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7136392.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGNAPSEMKEPYRLEGAMPEA
                     QLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETKKAFD
                     YAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLT
                     ETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIERGDI
                     DFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNPPKTI
                     TFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKLSGTS
                     MSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFSPRQQ
                     GAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYYQVTV
                     QTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKNGYFL
                     EGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQL
                     DGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTFAKQD
                     DDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNVVWTS
                     EVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISSGAKE
                     QHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDEDLPTT
                     EYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEGHSNK
                     PEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSSRTLE
                     KRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQKETKK
                     "
BASE COUNT         1644 a          906 c          908 g         1203 t
ORIGIN      
        1 cataccttag aaaatatcca attgaattaa ccgattttgc tttagatgcg gtaaaaaaat
       61 ataattctga taagcttgaa ttagtttttg atactgttga atcactgatt tgtgatgaac
      121 ttgaagatca agaatttcag aagtttaaga aaaaagtatt aaataatttc aaatatataa
      181 aaccagctca tcttagaaat ctttcaaatc gtggtattgg tatcatggaa tcacaacaca
      241 gaaagataac gtatagaatg aagcgacgtg gcatgtattg gtcaaagtgg ggaatctcca
      301 caatggcaaa tatgattata cttgaaagag ctaacggttt acgagaatta tttttcggtt
      361 cttggagaaa ggtatacagt gagtataaag aaggttcatt tagtgcaggg cgacttttta
      421 aaaagacaga tgaattagat aaattttcta agccccttct aaaaaatggc agaaaatgga
      481 gtataacagg aatcaaaaca aaatagaaaa agtctaaaga tacctttatt tctagcgttt
      541 ttttgcatgt tttaacttgc attaaatctt ctaaagtagt ataattatat taaagaaaag
      601 caaaaaaggt gaaggagcgc gaactcctac accaaaccga ttaagacaaa taccacttgc
      661 ttaatcagtc gaaccttatt gtagcataac taaaacaata ccgtcaagac tgaacaagca
      721 aaaaatactt gttttggtct ttttaagaat aattcaagtt ttcttaaatt tatgaggtat
      781 cgactaatct caaaatttca gacacatacc tgagtgaaca tcaagagaga accgatcggt
      841 tctctcttga tgtctaaaag aatgaggtta aggaggtcac aaactaaaca actcttaaaa
      901 agatgacctt tactaataat cgtctttgtt ttataataaa gatgttagta atataattga
      961 taaatgagat acatttaatc attatggcaa aagcaagaaa aatagctgtg tcatatgcaa
     1021 ataacccctg tttgctcttt aaaaaagacg ttatccttat ttctctacac acagacggat
     1081 agctaggaga gaatcgtttg attctctctt ttcttaatgg tcataaagac aaagtctctc
     1141 atcatgaaag gacgacacat tgcgtaaaaa acaaaaacta ccatttgata aacttgccat
     1201 tgcgcttata tctacgagca tcttgctcaa tgcacaatca gacattaaag caaatactgt
     1261 gacagaagac actcctgcta ccgaacaagc cgtagaaccc ccacaaccaa tagcagtttc
     1321 tgaggaatca cgatcatcaa aggaaactaa aacctcacaa actcctagtg atgtaggaga
     1381 aacagtagca gatgacgcta atgatctagc ccctcaagct cctgctaaaa ctgctgatac
     1441 accagcaacc tcaaaagcga ctattaggga tttgaacgac ccttctcatg tcaaaaccct
     1501 gcaggaaaaa gcaggcaagg gagctgggac cgttgttgca gtgattgatg ctggttttga
     1561 taaaaatcat gaagcgtggc gcttaacaga caaaactaaa gcacgttacc aatcaaaaga
     1621 aaatcttgaa aaagctaaaa aagagcacgg tattacctat ggcgagtggg tcaatgataa
     1681 ggttgcttat taccacgact atagtaaaga tggtaaaaac gctgttgatc aagaacacgg
     1741 cacacacgtg tcaggaaatg ctccatctga aatgaaagaa ccttaccgcc tagaaggtgc
     1801 gatgcctgag gctcaattgc ttttgatgcg tgtcgaaatt gtaaatggac tagcagacta
     1861 tgctcgtaac tacgctcaag ctatcagaga tgctgtcaac ttgggagcta aggtgattaa
     1921 tatgagcttt ggtaatgctg cactagctta cgccaacctt ccagacgaaa ccaaaaaagc
     1981 ctttgactat gccaaatcaa aaggtgttag cattgtgacc tcagctggta atgatagtag
     2041 ctttgggggc aagccccgtc tacctctagc agatcatcct gattatgggg tggttgggac
     2101 acctgcagcg gcagattcaa cattgacagt tgcttcttac agcccagata aacagctcac
     2161 tgaaactgct acggtcaaaa cagacgatca tcaagataaa gaaatgcctg ttatttcaac
     2221 aaaccgtttt gagccaaaca aggcttacga ctatgcttat gctaatcgtg gtacgaaaga
     2281 ggatgatttt aaggatgtcg aaggtaagat tgcccttatt gaacgtggcg atattgattt
     2341 caaagataag attgcaaacg ctaaaaaagc tggtgctgta ggggtcttga tctatgacaa
     2401 tcaagacaag ggcttcccga ttgaattgcc aaatgttgac cagatgcctg cggcctttat
     2461 cagtcgaaga gacggtctct tattaaaaga caatccccca aaaaccatta ccttcaatgc
     2521 gacacctaag gtattgccaa cagcaagtgg caccaaacta agccgcttct caagctgggg
     2581 tctgacagct gacggcaata ttaagccgga tattgcagca cccggccaag atattttgtc
     2641 atcagtggct aacaacaagt atgccaaact ttctggaact agtatgtctg caccattggt
     2701 agcgggtatc atgggactgt tgcaaaagca atatgagaca cagtatcctg atatgacacc
     2761 atcagagcgt cttgatttag ctaagaaagt attgatgagc tcagcaactg ccctatatga
     2821 tgaagatgaa aaagcttatt tttctcctcg ccaacaggga gcaggagcag tcgatgctaa
     2881 aaaagcttca gcagcaacga tgtatgtaac agataaggac aatacctcaa gcaaggttca
     2941 cctgaacaat gtttctgata aatttgaagt aacagtaaca gttcacaaca aatctgataa
     3001 acctcaagag ttgtattacc aagtaactgt tcaaacagat aaagtagatg gaaaacactt
     3061 tgccttggct cctaaagcat tgtatgagac atcatggcaa aaaatcacaa ttccagccaa
     3121 tagcagcaaa caagtcaccg ttccaatcga tgctagtcga tttagcaagg acttgcttgc
     3181 ccaaatgaaa aatggctatt tcttagaagg ttttgttcgt ttcaaacaag atcctacaaa
     3241 agaagagctt atgagcattc catatattgg tttccgaggt gattttggca atctgtcagc
     3301 cttagaaaaa ccaatctatg atagcaaaga cggtagcagc tactatcatg aagcaaatag
     3361 tgatgccaaa gaccaattag atggtgatgg attacagttt tacgctctga aaaataactt
     3421 tacagcactt accacagagt ctaacccatg gacgattatt aaagctgtca aagaaggggt
     3481 tgaaaacata gaggatatcg aatcttcaga gatcacagaa accatttttg caggtacttt
     3541 tgcaaaacaa gacgatgata gccactacta tatccaccgt cacgctaatg gcaaaccata
     3601 tgctgcgatc tctccaaatg gggacggtaa cagagattat gtccaattcc aaggtacttt
     3661 cttgcgtaat gctaaaaacc ttgtggctga agtcttggac aaagaaggaa atgttgtttg
     3721 gacaagtgag gtaaccgagc aagttgttaa aaactacaac aatgacttgg caagcacact
     3781 tggttcaacc cgttttgaaa aaacgcgttg ggacggtaaa gataaagacg gcaaagttgt
     3841 tgctaacgga acctacacct atcgtgttcg ctacacgccg attagctcag gtgcaaaaga
     3901 acaacacact gattttgatg tgattgtaga caatacgaca cctgaagtcg caacatcggc
     3961 aacattctca acagaagata gtcgtttgac acttgcatct aaaccaaaaa ccagccaacc
     4021 ggtttaccgt gagcgtattg cttacactta tatggatgag gatctgccaa caacagagta
     4081 tatttctcca aatgaagatg gtacctttac tcttcctgaa gaggctgaaa caatggaagg
     4141 cgctactgtt ccattgaaaa tgtcagactt tacttatgtt gttgaagata tggctggtaa
     4201 catcacttat acaccagtga ctaagctatt ggagggccac tctaataagc cagaacaaga
     4261 cggttcagat caagcaccag acaaaaaacc agaagctaaa ccagaacaag acggttcagg
     4321 tcaaacacca gataaaaaaa aagaaactaa accagaaaaa gatagttcag gtcaaacacc
     4381 aggtaaaact cctcaaaaag gtcaatcttc tcgtactcta gagaaacgat cttctaagcg
     4441 tgctttagct acaaaagcat caacaagaga tcagttacca acgactaatg acaaggatac
     4501 aaatcgttta catctcctta agttagttat gaccactttc ttcttgggat tagcagctca
     4561 tatatttaaa acaaaacgcc aaaaagaaac taaaaaatag tattacttaa actgataaga
     4621 gcttatttaa tagagagaac caatttggtt ctctctattt t
//
LOCUS       DATRSG010000096         4633 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRSG010000096 DATRSG010000000
VERSION     DATRSG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.098"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            115..1614
                     /locus_tag="VBD89_001900"
     CDS             115..1614
                     /locus_tag="VBD89_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7136393.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
     gene            1616..2332
                     /locus_tag="VBD89_001901"
     CDS             1616..2332
                     /locus_tag="VBD89_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO7136394.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            2432..3499
                     /locus_tag="VBD89_001902"
     CDS             2432..3499
                     /locus_tag="VBD89_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7136395.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVTELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            3511..3963
                     /locus_tag="VBD89_001903"
     CDS             3511..3963
                     /locus_tag="VBD89_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO7136396.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            4265..4429
                     /locus_tag="VBD89_001904"
     CDS             4265..4429
                     /locus_tag="VBD89_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136397.1"
                     /translation="MLLTSIDVNPVYANMAFDWVYDNYKDDIINYGQETIKNIGKSIS
                     ANNSLGTLYN"
BASE COUNT         1491 a          710 c          916 g         1516 t
ORIGIN      
        1 ttttcagaaa ataatattat ttcttgatta aaaaatcaaa aaataacctt ataagaccta
       61 tttgttattc ttaaaagata ttagttaaaa tcagaaatag agattggaga taaaatgact
      121 acaaaagcaa aacatgatat tcatggcaaa ccatataatc gtacagccat gatcacctta
      181 ttgttgattg ctacatttgc aggggtatta aatcagacaa gtttgggaac ggctatccca
      241 actttgatga atagctttaa catttcgttg tcaacggctc aacaagcaac aacatggttt
      301 ttattagcga atgggattat gattcctgtt tcagcttatt tagcgacacg tttttcaaca
      361 aaatggttgt atgtgacatc ctacgttgtt ttacttattg gactgttaat gacgacgtta
      421 gcacctacat ctaactggaa ccttttttta gtaggacgta ttattcaagc gatttctgta
      481 gggatttcta tgcctttgat gcaggttgtt atggtgaatg tttttcctcc agagcagcgt
      541 ggtgccgcta tgggacttaa tggtttggtg gttggtcttg ccccagctat tgggcctaca
      601 ttagcagggt ggattttaaa acaagaattt cattttgcag ggcatgattt aacatggcgt
      661 gcaattttcc ttcttccttt actaatttta acggtcacta caattttatc cccctttgtt
      721 ctaaaagatg tggttgataa taagtcagtt aaattggaag tgccttccct tatcctttca
      781 ataattggct ttggtagctt tttgtggggc ttcacaaatg tggcaactta tggatgggga
      841 gatattggat atgttatttc tcctattatg gttggtatta tttttatcgc cttattcatt
      901 catcgtcaat taaaactaga aacaccgttc ttagatatcc gtgttttcaa aaataaacaa
      961 ttctcagtaa caacagcagc tattgcactt tcaatgatgg cgatgatggg tgttgaaatg
     1021 atgctaccgc tttacttgca aaatgttcat ggtctctctg cacttgattc tggtttagct
     1081 ttattaccag gtgccttgat gatggggata gttagtccaa tttctggagc tgtatatgat
     1141 aaagttggtg caagacgtat ggctatgatt ggttttacca tactaggtgt agcgacgtta
     1201 ccttttgttt tcttaacgac tacaacacca gatcatttta ttaccctctt atatgcagta
     1261 cgtatgtttg gtattgctat ggttatgatg ccattgacag caagtgcgat gagcgcactt
     1321 ccaccacatg aggccgcaca tggaacagca gctaataata ctgctcgtca aattgcttca
     1381 gcagttgtag ttgcattgct ttcaagtgtt gctcaaaata ttatcaccaa caataaacca
     1441 tcaaaagatt tgctaacaat gaatccttta aaatatgcaa atcagatgtt gaatgctagt
     1501 cttgatggct tccatgtttc ttttgccatt ggttttgtat ttgcggttct tggtttactg
     1561 gtatctcttt tcctgagaaa agggaaaatt attgagacgg aaaaggaggt atagcatgat
     1621 tattttgatt attgcactgg caactatttt gacatttgtc acatggatgt tgattcgtcc
     1681 aaaagctcta cggtatattc taggtcttct ctcattagct ttgttgtttg cctcagtttg
     1741 gatgttaacg gatcacttta ttaatcatac aggtatgact attgaaacta agacaaagac
     1801 acgtgagatt tatagtgcgg gtgatagtaa ggctccattt ggtctcatga tttataaaga
     1861 gattggtact cattcgagta ataatgtttt agtttatcga acagatttaa atcaaaaaga
     1921 agcaaaagct cactttgttc caaatacaaa agaagtgaca agtgctgtga aaaaatctgc
     1981 tagctaccaa ttagctgatg tgaaaaaagc cacagtaaca acaaagacta aacgttatgt
     2041 gtggaaagat agtctttcga aactacttta tggctttgca ggtgaaaatg gagaattgat
     2101 ttctcagaaa tcagttgcta aaataccacg aaaaacttgg ctagttttga ctaaagagca
     2161 gtccgaacac ttagaatcgg ctgcggctaa actaaaagaa caaatgactt cgcaagcaaa
     2221 agtaaaccca gtgaaagcta tggagataaa gacattagct aagacaaatc cagaagctta
     2281 tgctgagatg caagttaaag cattaaaaga agctcttaat ataaaagaat agtaaagtaa
     2341 aagcaactga aatgagttgc ttttcttata gctaaatgtt ttaaaagatg ataaaattaa
     2401 cctaattaag gttagaatag cgaggtttct tatgggtttt gaacaacgtc taactagatg
     2461 tcaaacagca atttcacaat tatcatgtga cgcactctta ataacaaatt taacaaatat
     2521 tttttatttg actggttttt caggaactaa tgcaacggta ctcattagtc caaaacaccg
     2581 gatttttgtt acagattcac gatatgcctt gatagcaaag aatactgtaa gagagtttga
     2641 tattatcatt agcagagaac cacttgcggc tattttgaaa attattcgcg atgacgcatt
     2701 gatagcgatt ggttttgaaa ctgatatttc ttatcatatg tacaaacata tggtggaagt
     2761 ttttgaagat tatcgcttga tagaagctcc atctgttgtt gaaaagttac gtatgattaa
     2821 agatgtaaca gaacttgcga tcatccgtca agcctgtcgt atttcggacc aagctttttt
     2881 agatgtgttg gattttataa agcctgatcg aacaactgaa ttgcaagttg ctaatttttt
     2941 agactttcgc atgagagaat taggagcaac tggtccttca tttgatttta ttgtagcatc
     3001 tggatatcga tcagcgatgc cacatggggt tgcgagtcag aagactattc aatcgggaga
     3061 aactctgact ttagattttg gttgttacta tcagcactat gttagtgata tgacacgtac
     3121 gattcatatc ggtcatgtca ctgatcagga acgcgagatt tacgatatcg ttttgaaaag
     3181 taatcaagct attattgata atgtaaaatc aggaatgaag cgttgtgatt atgattactt
     3241 ggcgcgacaa gtcattgaaa attctggata cggtaatcat tttacccacg gtatagggca
     3301 tggtatgggg cttgatgttc atgaaattcc ttattttggt aagtcagagg gagtgattgc
     3361 atctgggatg gtagtgactg acgagccagg aatatatctc gataataaat acggtgttcg
     3421 tattgaagat gatttattaa taacggaaac aggttgcgaa gtattgacat ctgcaccgaa
     3481 agaattaatt gtactttaaa ggaaaataga atgaatagac tttcttggga agattatttt
     3541 atggcaaatg cagagttgat ttctaaacgt tctacctgtg accgtgcttt tgttggagct
     3601 gttttagtca aaaataatcg catcattgct acaggttata acggaggagt ctctgagaca
     3661 gataactgta acgaagtggg acattatatg gaagatggtc actgtattag gactgttcat
     3721 gctgagatga atgcccttat tcagtgtgct aaagaaggta tttctactaa taatacagaa
     3781 atttatgtga cgcattttcc ttgtatcaat tgcaccaagg cgctcttgca agccggggtt
     3841 aagaaaatta cttataaagc taattatcgc ccacacccat ttgctattga acttatggaa
     3901 gcaaaaggag ttgcttatgt tcaacatgat gttccagagg taacactagg gatggaagag
     3961 taaagaggaa gatagagtat atgcctatct tccttgaagg ttttatcgtt taagctaagg
     4021 gccagttatg ttaaatatat tgaacgagat aattctcaaa attggtcaat tgttagtcat
     4081 gggattactg aaaatggcct aaaaaaggtg atcaccagtt aatagtcatt attcctaatg
     4141 atttttcaca gaaagtttta gatgttgata atagaatagc tgatccatct attatcaaca
     4201 tctaaaatta gtactgctgg gaatctcgaa ttgcaaaaat aatttacaaa aaagagaaaa
     4261 gatattgttg ctgacctcaa tagatgtaaa tccagtttat gctaatatgg catttgattg
     4321 ggtgtatgat aactataaag atgatatcat caattatgga caagaaacta tcaaaaatat
     4381 tggtaaatcc atttcagcta ataatagttt aggtacttta tataattaag gagcaagatt
     4441 tgaaaacgga aatagaaaca aataatctta ttaatttagg atggtttcat gataatgctg
     4501 tttattatga tatgaaaacg aaaaaattat ataaatctaa tctaaaaaaa aaatagcaca
     4561 ggtataatgc tactaactcc tattatttca actattattg gaaaaataat tcaaagtata
     4621 agtgaaaatc tag
//
LOCUS       DATRSG010000097         4611 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.099, whole genome shotgun sequence.
ACCESSION   DATRSG010000097 DATRSG010000000
VERSION     DATRSG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.099"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(20..787)
                     /locus_tag="VBD89_001905"
     CDS             complement(20..787)
                     /locus_tag="VBD89_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO7136398.1"
                     /translation="MTDFSTALKVLVEQYSYHNAFLLLRKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
     gene            complement(796..1506)
                     /gene="deoD"
                     /locus_tag="VBD89_001906"
     CDS             complement(796..1506)
                     /gene="deoD"
                     /locus_tag="VBD89_001906"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7136399.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            complement(1490..2746)
                     /locus_tag="VBD89_001907"
     CDS             complement(1490..2746)
                     /locus_tag="VBD89_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO7136400.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            complement(2748..3557)
                     /locus_tag="VBD89_001908"
     CDS             complement(2748..3557)
                     /locus_tag="VBD89_001908"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7136401.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            complement(3596..4003)
                     /gene="arsC"
                     /locus_tag="VBD89_001909"
     CDS             complement(3596..4003)
                     /gene="arsC"
                     /locus_tag="VBD89_001909"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO7136402.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            complement(4054..>4611)
                     /locus_tag="VBD89_001910"
     CDS             complement(4054..>4611)
                     /locus_tag="VBD89_001910"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7136403.1"
                     /translation="ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHD
                     MGHNKSNSHGIDTLIKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDER
                     LPEIISAMRDKDLLLITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFAD
                     ISATVADNFGVDTAMIGESFLQDLV"
BASE COUNT         1499 a          913 c          774 g         1425 t
ORIGIN      
        1 tctcagaact gtttttaaat tactcaagta tctttataat aagcttgtta accgtatcgt
       61 agtgaaaatt aacagtatca tactcaacac caattacctt tgccaagaag ataatctggt
      121 ctaaaaatgc ttgagaagaa aattgagttg tacgatatag ttcttgctca tcaaaaggtt
      181 taatcaggtg agccaaagga tttctgactg atttttcgaa ctgtcttaat tgaatgatgg
      241 tcgatttcac ttcttcatca aggtcggtta attgtaaaca ttctgcaagg cttttagaag
      301 tcaagcgact atcgtgccac ctttcagaga atgccaaaag gactggatgg tttgactcct
      361 ttaattcttt aaacttccaa gtatcgtagt gatcatctct tgcattatag atgtaatcat
      421 gcaagtgagg tacttcttgt gcaagtaaaa tcataaatag acgatataaa ataggactaa
      481 cactacggac aaaatctatg attaatccat tttttacttt tgcttctaaa tccatgatgt
      541 aatttgccag caactctaaa tcatacgagt tatgcaaaag ctttatattg tatttttctt
      601 gtaatataac aacttgttct tgatgagcaa aaagaaaatc aatgtttaac tctcgacgct
      661 ctttcatcat ttctaataaa aataataaat ctgagtttaa aggaccatgt tttcgtaata
      721 ataagaaagc attatgataa gaatactgtt ccactaatac tttaagagca gttgaaaaat
      781 ctgtcatttc tatcctcatt cagaaataag tgtttcgagc ccaactttca tcatatcagt
      841 aaatgttgtt tgacgctctt ctgcacttgt atcttcttct ggattattca agttgtctga
      901 aatcgtcatc atagcaagag cattaacatt gtgttgagct gcaagatagt agagagctgc
      961 tgcttccatc tcaatagcat gaacacctag tttacccaaa gccatattac gatctggttg
     1021 gtttgaataa aacacgtctg aagataatac actgcctaca tgagttgtaa tatccatttc
     1081 ctttgcgatg tgataagctt tatccaaaag tttaaaatca gcaatttgcg gaaaatcaaa
     1141 ttcaggccaa tcattacgga taatattaga atttgttgct gctgcttgtg ccaaaacaag
     1201 ttcacgaaca tggatatcag gattaatagc acctgctgtt cctacgcgaa tcaatgtttt
     1261 aaccccataa tcaacaatta gttcgcgagc ataaattgaa attgaaggca tacccatacc
     1321 agttcccata atagaaacac gatgtccttt ataggtacct gtataaccaa acatattgcg
     1381 aactgtatta aaacacaccg catcttctaa aaaattctcg gcaataaatt tcgcacggag
     1441 tggatctcct ggaagtaaaa ttttatctgc aatttcacct tgttttgctt caatatggat
     1501 tgacatataa actcctcatt tttcttataa aataatcata ggcagtagca aaaagtactg
     1561 ctatagttag gggaacaata gttataacag attgaccagt aaaagatatg actaaaccga
     1621 cagctgtcag tggcgctctc attgtaatag ctaaaaaaat agctgcacca accaacatac
     1681 tggttactat cgataggtgg ggaagtaaag gaatagaaat actagctaac aaaaaaccta
     1741 aacaagcacc aatggaaaaa gatggtgtca gtataccgcc ataagcacca gcgtaaagag
     1801 tggataatgt tgctatcatc ttgagaacaa ctaaaatagc gattaagcta actgtggtcc
     1861 ctttaaagac ttcttgaact agggcattac catttcctaa aatctgggga tagattattg
     1921 ataaaacaga tactatcaat gacactactg gcaaacttaa caatattttt atatttttaa
     1981 tacgtctctc tgtcactttt tgatttaaat atctaaagct aatcgcaatc ggagttacac
     2041 ataaaacgat caagaacata aaagctaatg actgtgaatt cagactcatc tttgaaatat
     2101 gataaagagg ggctgtcgaa ataactaatt gagcaatact agctgcacca aaagtagaga
     2161 ctagcaatgt cacaatattt ttcagtgaaa ttcctaaagc taaagtttca aaagcaaaga
     2221 atacactagc taagggtact tgataaactg ctgacaaacc tgcagctgca ccactaacga
     2281 ttaataaacg tttatccagc actttaagtg ctaataaatc cgatattttc ccagcattta
     2341 tggctccaaa ctctcgtggc gctccttctt ttccaatagg accaccagct cctacataaa
     2401 tcaattgcaa aaatgcgtga cctatatgta gccagtaagg tgattttttt acagaaaaat
     2461 ccttaaaaac aacctgttgt ttaatactag ttatttgctt tggcttgatt tgtaagtaat
     2521 accaactaag ggatgctaaa aaggagacta aagtaagtga taacactctt ttaattggtg
     2581 tgacactatt taacaaagac agagtatttt ctctactatc accaaatgct aaccactctg
     2641 taaacattaa tacatagtgc attaagatag ctccaactcc tgccataact gtcataaata
     2701 gaacggctat cataaacttg acagcatagt ataatcgaga aaccatttta aagttcagca
     2761 actaatgatt taactaatcc cttgaaatct tctttaatac gctgagtaac ttcaacgacc
     2821 tcctcatgat tgagctctga ttggaagcca gcggcaaagt tagtaattgc tgaaattcct
     2881 aacactttaa gccctgagtg agctgcaacg atcacctctg gaaccgtgga catacctacc
     2941 gcttgtgcgc ccattgtttg gaatgcacga atttctgcag gtgtttcata agtgggtcct
     3001 gatacaccca agtacacacc ttcttcgagt ttgatattct gtttttcagc aatttggtga
     3061 gctttttgtc gatatgttgc tgtataagca tccgacatgt ctgggaaacg tggtccaaat
     3121 tcttcaaggt tctcacctat gagagggtta gtcccaatca tattgatgtg gtctttgatc
     3181 agcattaaag ttcctggtcc gtatccaatc ccacccgctg cattagtcac aagcacactg
     3241 tggcaagcca atgctctcat gatacgtact gggaaagtaa cgacttccat tgtatttcct
     3301 tcataaaagt gaaaacgacc ttgaagcgct aataccttac ggcctgataa atccccatac
     3361 actaattttc cagcatgacc aactactgtt gactgtcccc aatttgggat gtctgcataa
     3421 tccacaacaa tgggattttc gatttcttca gctaattctc ctaaaccaga gcctaaaata
     3481 agaccaaatt ctggtgctgt gacgcctttt gcttgcaaaa agtctctagt ctcattaatt
     3541 ttttctaata atgtcattta cttacctcct aaactattat tcactataca atatgctata
     3601 aactttggat aatctgccca tcttctttaa caaaatcttt ttccatcttt acaggtaaaa
     3661 tggttaaaat ttcttcacag ggtcggcaga gttttgcacc tttactcgta acaactatgg
     3721 gacgattgat aagaacagga tcttgtatca tcgcatcaat catttcttca tctgtaacgc
     3781 tagaagattc caaattgaat ttctcataag caggaacatc tgttcttaga agttctctca
     3841 cagaaagttt catttctaac aacagttcaa ctagtgccat cttgctaggt ggtgttttca
     3901 gataataaat aatttctggt tcaataccac aatgacgaat aatggcaagt acgttccttg
     3961 atgtcccaca atttgggtta tggtaaatcc taactttttc catcatcttc ctctcaattt
     4021 ttaatgctac aagtgtctta aggaaataaa aatttatacc aagtcctgca agaagctctc
     4081 tccaatcatc gcagtatcaa caccaaagtt atccgcaact gttgctgaaa tatctgcgaa
     4141 atgccctact gggataaggc cgtttcctgt gaaagatggg ctataggcta agagaggaat
     4201 gtattctctt gtatggtctg tacctgcata agttgggtca ttaccatgat cggcagtgat
     4261 caaaagtaag tctttgtcac gcattgctga aatgatctct gggaggcgct catcaaactc
     4321 atgaagacaa tcacgatagc catgaggatc acgacggtga ccatagagag catcaaagtc
     4381 aaccaaatta gtaaaggaga agcctttttc aaactctgat aaccccattg tttttatcaa
     4441 agtatcaata ccatgactat tcgatttgtt atgtcccata tcatgattaa tgcctgaacc
     4501 attgaaaata tcattgattt taccaacagc ataagtatcg atgccagctt gatctagttt
     4561 atttaatacg gtatcttcaa atggtgatac tgcatagtcg tgacggttgg c
//
LOCUS       DATRSG010000098         4599 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRSG010000098 DATRSG010000000
VERSION     DATRSG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.100"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            73..923
                     /locus_tag="VBD89_001911"
                     /pseudo
     CDS             73..923
                     /locus_tag="VBD89_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
     gene            1010..1453
                     /locus_tag="VBD89_001912"
     CDS             1010..1453
                     /locus_tag="VBD89_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO7136404.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            1456..2166
                     /locus_tag="VBD89_001913"
     CDS             1456..2166
                     /locus_tag="VBD89_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7136405.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            2156..2968
                     /locus_tag="VBD89_001914"
     CDS             2156..2968
                     /locus_tag="VBD89_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7136406.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILMF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(3179..4209)
                     /locus_tag="VBD89_001915"
                     /pseudo
     CDS             complement(3179..4209)
                     /locus_tag="VBD89_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT         1346 a          649 c          886 g         1718 t
ORIGIN      
        1 catcttaatt gtccgaattt gtcatttgtt atgttataat atggatattc tatattttat
       61 tagggaggtt ttatgaaaat ttttgagaaa gctcctgcca aacttaattt aggattagat
      121 attaaaggac gatgtgacga tggttatcat gaattagcta tgattatggt tagtattgat
      181 cttaatgact acgttaccat ttctgagcta aaggaagatt gtattgttat cgattctgat
      241 agtagcaaaa tgcctttaaa taatgataat gatgtgttta aagctgcaga tataatcaaa
      301 aatcaatatg gtattaataa aggtgttcat attagattag aaaaatccat tcctatctgt
      361 gcaggactgg gggggggtct actgatgctg ctgctacaat aagagctctt aatcgattat
      421 ggaatcttca aatgggccat gacgaaatgg ttgctattgg ttttaaaatt ggtagtgatg
      481 ttccttattg ccttggtggg ggatgttcgt tagtattagg taaaggtgaa attgttaaac
      541 ccttaccgac attgagacca tgttggatag ttctggttaa gccggatttt ggtatttcaa
      601 ctaaatccat ttttagagat attgattgta agtctataag cagagttgat attgatttat
      661 taaagtctgc tattttgtca tcagattatc agttaatggt caaatctatg ggaaattcac
      721 tcgaggatat tacaattact aaaaatccag ttataagtac aataaaagaa cgtatgttaa
      781 attctggagc cgatgttgca ttaatgacag gaagtggacc aactgttttt tcaatgtgtt
      841 ctacagagaa aaaagctgat cgtgttttta atagtatgaa aggtttttgc aaagaagttt
      901 ataaagttag gttgttgaga taaaaggttt tatcttcttc acctatctat gattgattct
      961 tctatatctt cgtgatataa tttactggtt aacaaaaaaa gaggtatata tgacagtttt
     1021 agaacaaaaa ttagaccatt tagtgagtca aatcttgctt aaggcagaaa atcaacatga
     1081 attattgttt ggtacttgtc aaagtgatgt aaaattgact aatacgcaag aacacattct
     1141 gatgctttta tcacaagagc agttgacaaa ttctgattta gctaagaagt taaatattag
     1201 tcaggcagct gtaactaaag cagtaaaaag tttaatttct caggatatgt tgaaagcgaa
     1261 taaagattct aaagatgctc gtattactta ttttgagtta tctgaacttg ctaaacccat
     1321 tgcagatgaa cacacacatc atcatgacaa tacgttaggg gtttacggaa gattagtaaa
     1381 tcatttttca aaggacgaaa aggtcgtttt agaacgtttt cttgatcttt tctctagaga
     1441 attagaaggg taatcatgcg atatattaca gttagtggtc ttacttttca gtatgatagc
     1501 gatcctgtgc tggaaggggt taattaccat ttagacagtg gggagtttgt taccttaaca
     1561 ggtgaaaatg gggctgctaa atcaacttta ataaaggcta ctttaggtat tttaacgccc
     1621 aaagtgggta cagttaatat ctctaaagaa aacaaagaag gaaaaaaatt acgtattgcc
     1681 tatttacctc aacagattgc aagttttaac gctggctttc catcgtcagt ttatgaattt
     1741 gtgaaatctg gacgataccc tagaaatggt tggtttagaa gactgactaa acatgatgaa
     1801 gaacatatta gggttagttt agaagctgtt ggtatgtggg ataatcgcca taaaaagata
     1861 ggaagtttat ctggcggtca aaagcagagg gctgtcattg ctaggatgtt tgcttctgat
     1921 ccagatattt ttgtattgga tgagccaaca actggtatgg acgcagggac tactgagaaa
     1981 ttttatgagt taatgcatca taatgctcat aagcatggta agtctgtttt gatgattact
     2041 cacgatcctg atgaagtaaa ggggtatgct gaccgaaata ttcatcttgt taggaaccaa
     2101 tctttaccat ggcgttgttt caatgtccat acaaacgaaa tggaggttga atcagatgct
     2161 acttgatatg ctttcctatg attttatgca acgagcgctc ttagcagttg tggctattag
     2221 tatttttgct ccaattttag gcatcttcct aattttacgt cgtcagagtt tgatgagtga
     2281 tactttgagc cacgtttcct tagccggtgt tgcattaggc gttgttttag gtatctcgcc
     2341 tacgtggtca actattttcg ttgttacttt ggcggcagtt gtattagagt atttgcgtac
     2401 tgtatacaaa cattatatgg aaatatcaac tgctatcctg atgtcgatgg ggttggctat
     2461 atctttaatc gttatgagta aagctcataa tgttggtaat gtcagtcttg aacagtattt
     2521 atttggttct attatcacta ttgggaagga acaagtgatt gctttgtttg ttattgcatt
     2581 aatcactttt attttaacca tcctatttat tagaccaatg tatatattaa tgtttgatga
     2641 ggatacagct tttgtagatg gattgcctgt gcgtacaatg tctattttgt tcaatgttgt
     2701 tacaggtatt gctattgcgt taacaattcc tgccgcaggt gctctattgg tgtcaactat
     2761 tatggtttta ccagctagta ttgctatgcg gttaggtcga aattttaaaa ctgttatttt
     2821 tcttgggatg ttaattggat ttgtgggaat ggtagctggt attttccttt cttattattg
     2881 ggagacacct gctagtgcaa caataacgat gatttttatt ggcattttcc tattagttag
     2941 cttagtaggc ttgcttcgca aacgttaaat aaaaaaatct atcttattct aggatagatt
     3001 ttttatttat ctccctgttt ttttcgaata tattttatga gggatcagtt gagaaattat
     3061 ctcgatgagg tatctactaa gttttctggc ttaagttgat agaaggggca atgtgaatct
     3121 atttttgcgt tttaggataa aaaatactac cggcaatttt gccggcagta aattgtaatt
     3181 attgtgcttc agtagtgctg tgatatttgt gttgatttaa cgcttcttgt tcagtcattt
     3241 gaacgatatt agcggtattt gcatttttta tgttttctaa actataccaa taagtatttg
     3301 aacgaccctt atttgcaacg tagacgattc tatttttggg agctacatta tttgtatctg
     3361 attcagaagt attagtgtta gcattttctt ctatatcaga tgcaccttca atagctgctt
     3421 cgttttcatc agcatcgcta tttggatctt caccttcttc tgcctgtttc ccatcttctt
     3481 tgattagcat accagttttg taatcaattt ttgcgagtgg agacgtattt tctaatgtaa
     3541 cagttgtgac gccgtattca tcaacttgtt ctttgccgcc gcctaatttg atttctagaa
     3601 gttttccttg tttatcaatt ccaacatact gaagttcaac tcggctaggt actaaattat
     3661 ttccttcgta tattggagtc actttgtaat caagctagaa atttttatga tttgccaacc
     3721 aactatctaa tctattttcg tagtataaca taccatcagg attattttcg ctaaatcctg
     3781 tatttagata tttggtcata gtaactaggt tctcaggaac attgttcatt cctgaaaatt
     3841 gataaccaac gaggtgacct cggtctataa gccaagttgt tttacccttt tcagtagtaa
     3901 atttgtagtt gtgccaaccg gttggattat acgtcagtct ttctcttttt atcttaggct
     3961 catctgcatc ccttaattgt atgtgagaat atgttgctct ttctaagtta tctaatgaac
     4021 ctaccttgat ttgcttatta tgagtaaacg gtaagatttt tttgaagaag tacttacata
     4081 ctgtgtctca acatgggatg tcgtcttata atgtttagcc ttagcttcaa cattctgttg
     4141 taatgcccca acgcatagta cgacagttaa tagtgggagt gatttgtata attgtttaga
     4201 taatttcata aacctaaacc cttctaaatg atatttgtga aggcctttca caagtataat
     4261 tttatcattt tattgggaaa aagtaataaa aaaacataac tagttaattt ttttggagat
     4321 tatgttattt aactagatag ttattaaagt taaatgttat agatatactg ttattacata
     4381 ataattttta tatgatttgt tattttatta gattttttaa tggattattt ctttagctga
     4441 ttttgattac aaagttaagg tattctaatc atattttctt gatttataac atgtaaaggt
     4501 tagaataaat tcgttggtgt tagacagacg atatttgttg tttttatgaa aaaattgtgg
     4561 gtattagata tgtatatttt ttcgataact atttactct
//
LOCUS       DATRSG010000099         4162 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRSG010000099 DATRSG010000000
VERSION     DATRSG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.101"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(47..1609)
                     /gene="guaA"
                     /locus_tag="VBD89_001916"
     CDS             complement(47..1609)
                     /gene="guaA"
                     /locus_tag="VBD89_001916"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO7136407.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1822..2520
                     /locus_tag="VBD89_001917"
     CDS             1822..2520
                     /locus_tag="VBD89_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7136408.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            2634..3968
                     /gene="trmFO"
                     /locus_tag="VBD89_001918"
     CDS             2634..3968
                     /gene="trmFO"
                     /locus_tag="VBD89_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO7136409.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            4046..>4162
                     /locus_tag="VBD89_001919"
     CDS             4046..>4162
                     /locus_tag="VBD89_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7136410.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIITSERYFSNYISY"
BASE COUNT         1369 a          737 c          804 g         1252 t
ORIGIN      
        1 aagaaataag agtaaaaaaa cttagctact taaagtagct aagtttttat tcccactcaa
       61 cagttgcagg tggtttactt gtaatatcat aaacaatacg gttaacgtga tcaacttcgt
      121 taacaatacg tgtcgaaatt ttcttaagta catcccaagg aagttgcgca aagtcagctg
      181 tcatgccatc aattgaagta attgcacgga tagcaattgt atagtcataa gtacgaccat
      241 ctcccataac accgacagaa cgtacgccag tattaactgt gaaatactgc catacgtcac
      301 gatctagacc agctttagcg atttcttcac gaagaatagc atctgattcg cggactgttt
      361 caagtttttc ttcggtaatt tcacccataa cgcgaatagc aagaccaggc ccagggaatg
      421 gttgacgcca tacaacttca tcaggcattc caagtgcagt accaagagcg cgaacctcat
      481 ctttgaataa tgtgttaagt ggctcaatca attcaaattg catatcttct ggcaatccac
      541 caacattatg gtgtgacttg attgtttggg ctgtttctgt cccagattca ataatatctg
      601 tgtagagggt accttgagca aggaaatcaa cgcctttaag tttacttgcc tcatcatcaa
      661 aaacgtacac gaactcattt ccgatgattt ttcgttttct ctcgggatct tcaacaccag
      721 aaagaagatc taggaagcgt tttgaagcat caacacggat aatattaagt ccaaatttac
      781 caccaagcat atccataact tggtcacctt catttttacg taataaacca tgatctacaa
      841 aaatacatgt taattgatca ccgatagcac gttgtaggag tactccgaca acagatgaat
      901 caacaccgcc agatagtcca agaagtactt tacgatcgcc tacagtttca cgtatttttg
      961 caatttccat atcaatgaag ttatccattg accaatcacc acgtgcacca cagatattaa
     1021 cagcaaaatt tttaaggata tcattgccat atactgagtg acgaacttcg gggtggaatt
     1081 ggataccata aaattgtttt tcagtatttt ccattgcagc aaatggacag tcaacagagt
     1141 caccaacgag atggaaaccc tcagggattt cagtaacagc atcaccatgg ctcatcaaaa
     1201 caagttgttc ttgaggagtg ccagcaaaga gtgctgattc tgagcgtagg cgaagtgctg
     1261 attgaccata ctcacggtga ccagcttctc ccgcaggaag aaccttacca ccgagtttat
     1321 gggtaatcag ttgcatacca taacaaattc ctaagattgg aattcctaat tcaaaaattt
     1381 cttcatcgat tccaaaagcg ccatcagcgt atacggaatt tggaccacct gaaagtacga
     1441 taccaatagg attaatatcg cgaatttcat cagccgtaat tttatgtgat tttaactcag
     1501 aaaatacacc aaattcacgg atacgacgtg cgataagctg gttatattga ctaccgtaat
     1561 ctagaacgat aattttttgg atatcattca atatagaaat atcagtcatt gtttcccttt
     1621 ctttgtagct aattttaaaa acagctgtac ctataccatt ttagcatatt ttatataaat
     1681 taacatcatt aaaataattg attaaacgct ttcttatcaa gaaatattgc ttatgatttt
     1741 tctgaattat ttcacgattt tagagttaga aatatataga agtatggtaa aatgaagtgt
     1801 agattctagg aggagagaat attgttacca gcatatatta aaattcacga cgctattaaa
     1861 aaggaaatcg ataaagggac ttggaagatt gggcagcggt tacctagcga aagagattta
     1921 gcggatgatt atagtgtaag tcgtatgaca cttcgccaat caattacatt gttagttgaa
     1981 gaaggtattt tggagagacg agttggtagt ggcacctatg ttgctagtca tcgtgttcaa
     2041 gaaaaaatgc ggggaacaac tagttttaca gaaattgtca attctcaagg gcgtaagcca
     2101 tccagtaaac taatctcatt tcaaagaaaa ctagctaatg aaaccgaaat tcaaaaatta
     2161 aatttatcac agtcagatta tgtggttcgt atggaaaggg tacgttatgc tgataaggta
     2221 ccgttggttt atgaggtagc ctctatacca gagaatttaa ttaaaggttt tgaacaatca
     2281 gaggttacag aacatttctt taaaacctta acagagaatg gctacgaaat tgggaaaagc
     2341 caacaaacta tatatgctag aaatgctagt gaacgtgttg cttcgcattt agaagttaac
     2401 gcaggccatg ctattttggc attgacacaa gtttcatact ttactgatgg taagcctttt
     2461 gaatatgttc atgggcaata tgttggagat cgttttgagt tttatttaga gaataactag
     2521 tagtttaatg aataacgttt tttgaagaag tagctatgta tgttataatg gaaaaataaa
     2581 ttatatagaa agatttgagg aagtagttga ctactttcaa gaggtattag ttcatgtctc
     2641 aatcttacat taatgttatc ggcgctggtt tggctggttc tgaagcagct tatcaaattg
     2701 ctaaacgtgg aataccagtt aagctttatg aaatgcgtgg ggttaaatca acccctcaac
     2761 ataaaacaga taattttgca gaattagtat gttccaattc attccgcggt gattctctaa
     2821 caaatgcagt tggtctttta aaagaagaaa tgcgtcgttt ggattctatc attatgagaa
     2881 atggggaggc acatcgagtt ccagcaggtg gagccatggc cgttgataga gaaggttatt
     2941 ctgaagctgt tactgaggaa atccataaac atccattaat cgaagtaatt cgtgatgaaa
     3001 tcacagatat ccctggagat gctattacag ttatcgctac aggaccatta acaagtgata
     3061 gtctggcagc taaaatccat gaacttaatg gtggtgatgg tttttatttt tacgatgccg
     3121 ccgctccaat tgtcgataaa aatacgattg acatgaataa agtttacctt aagtctaggt
     3181 atgacaaagg tgaagcagct tatctaaatt gtccaatgac caaagaagaa tttatggctt
     3241 ttcatgaagc tttaactaca gctgaggaag caccactaaa ttcttttgag aaagaaaaat
     3301 acttcgaagg ttgtatgcct atcgaagtga tggctaagcg tggtattaaa acgatgcttt
     3361 atggtccaat gaaaccagtt ggtttggagt atcctgagga ctacaaagga ccgcgagatg
     3421 gtgaatttaa aacaccatat gctgttgttc aattacgtca agataatgct gcaggctctc
     3481 tttataacat agttggtttc caaactcatc ttaagtgggg agaacaaaaa cgtgtatttc
     3541 aaatgattcc tggtcttgaa aatgctgaat ttgtccggta tggtgttatg catcgcaatt
     3601 cttatatgga ttcaccaaat cttttaaacc aaacctttgc tacacgtaag aacccaaatc
     3661 ttttctttgc tggacaaatg accggtgttg aaggttatgt agaatcggca gcaagtggtc
     3721 ttgtagcggg aatcaatgct gtccgtcgct tcaatgggga gagtgaagta gttttcccac
     3781 aaactactgc tattggtgca ctgccacatt atattacaca tacagacagc aaacatttcc
     3841 aaccaatgaa tgttaacttt ggtatcatca aagaattaga aggtccacga attcgtgata
     3901 agaaagagcg ttatgaagct attgctacac gagctttgaa agatttagaa aaatttctca
     3961 actattgata tattcatttg actttgccaa caagcaaggt caaattttct atattgattt
     4021 ttaaaaatgt atgaatggag taaatatgca ctataaagaa ttacttgagt catccgaaaa
     4081 agcttatttg gaaactaatc ggtataaaca ttttaagaat ataatcactt cagagcgtta
     4141 tttttcaaac tatattagct ac
//
LOCUS       DATRSG010000100         4097 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRSG010000100 DATRSG010000000
VERSION     DATRSG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.102"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            98..1646
                     /locus_tag="VBD89_001920"
     rRNA            98..1646
                     /locus_tag="VBD89_001920"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1701..1773
                     /locus_tag="VBD89_001921"
     tRNA            1701..1773
                     /locus_tag="VBD89_001921"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1734..1736,aa:Ala,seq:tgc)
     gene            1928..>4097
                     /locus_tag="VBD89_001922"
     rRNA            1928..>4097
                     /locus_tag="VBD89_001922"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1177 a          822 c         1184 g          914 t
ORIGIN      
        1 gacgaacgaa tgtgcagggt ggatttacat tgtaaatccg tcaatgacaa aaaacaataa
       61 atctgtcagc gagacagaaa agagcaaagc tcaaactttt aatgagagtt tgatcctggc
      121 tcaggacgaa cgctggcggc gtgcctaata catgcaagta gaacgctgag gtttggtgtt
      181 tacactagac tgatgagttg cgaacgggtg agtaacgcgt aggtaacctg cctcatagcg
      241 ggggataact attggaaacg atagctaata ccgcataaga gtaattaaca catgttagtt
      301 atttaaaagg agcaattgct tcactgtgag atggacctgc gttgtattag ctagttggtg
      361 aggtaaaggc tcaccaaggc gacgatacat agccgacctg agagggtgat cggccacact
      421 gggactgaga cacggcccag actcctacgg gaggcagcag tagggaatct tcggcaatgg
      481 acggaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaagctct
      541 gttgttagag aagaacgttg gtaggagtgg aaaatctacc aagtgacggt aactaaccag
      601 aaagggacgg ctaactacgt gccagcagcc gcggtaatac gtaggtcccg agcgttgtcc
      661 ggatttattg ggcgtaaagc gagcgcaggc ggttctttaa gtctgaagtt aaaggcagtg
      721 gcttaaccat tgtacgcttt ggaaactgga ggacttgagt gcagaagggg agagtggaat
      781 tccatgtgta gcggtgaaat gcgtagatat atggaggaac accggtggcg aaagcggctc
      841 tctggtctgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc
      901 tggtagtcca cgccgtaaac gatgagtgct aggtgttagg ccctttccgg ggcttagtgc
      961 cgcagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag
     1021 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag
     1081 aaccttacca ggtcttgaca tccttctgac cggcctagag ataggctttc tcttcggagc
     1141 agaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
     1201 ccgcaacgag cgcaacccct attgttagtt gccatcatta agttgggcac tctagcgaga
     1261 ctgccggtaa taaaccggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgac
     1321 ctgggctaca cacgtgctac aatggttggt acaacgagtc gcaagccggt gacggcaagc
     1381 taatctctta aagccaatct cagttcggat tgtaggctgc aactcgccta catgaagtcg
     1441 gaatcgctag taatcgcgga tcagcacgcc gcggtgaata cgttcccggg ccttgtacac
     1501 accgcccgtc acaccacgag agtttgtaac acccgaagtc ggtgaggtaa ccttttagga
     1561 gccagccgcc taaggtggga tagatgattg gggtgaagtc gtaacaaggt agccgtatcg
     1621 gaaggtgcgg ctggatcacc tcctttctaa ggataaggaa acctgccatt tgcgtcttgt
     1681 ttagttttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc
     1741 aggaggtcag cggttcgatc ccgctaggct ccattgaatc gaaaggttca aattgttcat
     1801 tgaaaattga atatctatat caaattccac gatctagaaa tagattgtag aaagtaacaa
     1861 gaaaataaac cgaaaacgct gtgaatattt aatgagtttt ctagttttaa agaaactagt
     1921 ttaataaggt taagttaata agggcgcacg gtggatgcct tggcactaga agccgaagaa
     1981 ggacgtgact aacgacgaaa tgctttgggg agctgtaagt aagcgctgat ccagagatgt
     2041 ccgaatgggg gaacccacta gttaatgact agtatccata tctgttaagg atatgagaag
     2101 gaagacgcag tgaactgaaa catctaagta gctgcaggaa gagaaagcaa aagcgattgc
     2161 cttagtagcg gcgagcgaaa cggcaggagg gcaaaccgaa gtgtttacac ttcggggttg
     2221 taggactgcg atataggatt aatcattata gaagaattac ctgggaaggt aagccaaaga
     2281 gagtaacagc ctcgtattta aaatgataga ttaaccttag cagtatcctg agtacggcga
     2341 gacacgcgaa atctcgtcgg aatctgggag gaccatctcc caaccctaaa tactctctag
     2401 tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccggg aggggagtga
     2461 aatagaacct gaaaccgtgt gcctacaaca agttcgagcc cgttaatggg tgagagcgtg
     2521 ccttttgtag aatgaaccgg cgagttacga tatgatgcga ggttaagttg aagagacgga
     2581 gccgtaggga aaccgagtct taatagggcg ccatagtatc atgttgtaga cccgaaacca
     2641 tgtgacctac ccatgagcag gttgaaggtg aggtaaaact cactggagga ccgaaccagg
     2701 gcacgttgaa aagtgcttgg atgacttgtg ggtagcggag aaattccaaa cgaacttgga
     2761 gatagctggt tctctccgaa atagctttag ggctagcgtc gatgttaagt ctcttggagg
     2821 tagagcactg tttgggtgag gggtccatcc cggattacca atctcagata aactccgaat
     2881 gccaacgaga tataatcggc agtcagactg cgagtgctaa gatccgtagt cgaaagggaa
     2941 acagcccaga ccaccagcta aggtcccaaa atatatgtta agtggaaaag gatgtggggt
     3001 tgcacagaca actaggatgt tagcttagaa gcagctattc attcaaagag tgcgtaatag
     3061 ctcactagtc gagtgaccct gcgccgaaaa tgtaccgggg ctaaaacata ttaccgaagc
     3121 tgtggatgac acaaaagtgt catggtagga gagcgttcta tgtgtgaaga aggtgtaccg
     3181 tgaggagcgc tggaacgcat agaagtgaga atgccggtat gagtagcgca agacaggtga
     3241 gaatcctgtc caccgtaaga ctaaggtttc caggggaagg ctcgtccgcc ctgggttagt
     3301 cgggacctaa ggagagaccg aaaggtgtat ccgatggaca acaggttgat attcctgtac
     3361 tagagtatat agtgatggag ggacgcagta ggctaactaa accagacgat tggaagtgtc
     3421 tggtcaaaca gtgaggtgtg atatgagtca aatgcttata tctttaacat tgagctgtga
     3481 cgaggagcga agtttagtag cgaagttagt gatgtcacac tgccaagaaa agcttctagc
     3541 gatacatata ctctacccgt accgcaaacc gacacaggta gtcgaggcga gtagcctcag
     3601 gtgagcgaga gaactctcgt taaggaactc ggcaaaatgg ccccgtaact tcgggagaag
     3661 gggcgctgat tttagatcag ccgcagtgaa taggcccaag caactgttta tcaaaaacac
     3721 agctctctgc taaatcgtaa gatgatgtat agggggtgac gcctgcccgg tgctggaagg
     3781 ttaagaggag tgcttagcgt aagcgaaggt atgaattgaa gccccagtaa acggcggccg
     3841 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa
     3901 aggcgtaatg atttgggcac tgtctcaacg agagactcgg tgaaatttta gtacctgtga
     3961 agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg cagtttgata
     4021 ttgagtatct gtaccacatg tacaggatag gtaggagcct atgataacgg gacgccagtt
     4081 tcgtttgagg cgttgtt
//
LOCUS       DATRSG010000101         3857 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRSG010000101 DATRSG010000000
VERSION     DATRSG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.103"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            49..1586
                     /locus_tag="VBD89_001923"
                     /pseudo
     CDS             49..1586
                     /locus_tag="VBD89_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1763..3307
                     /locus_tag="VBD89_001924"
     CDS             1763..3307
                     /locus_tag="VBD89_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO7136411.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            3393..3773
                     /locus_tag="VBD89_001925"
     CDS             3393..3773
                     /locus_tag="VBD89_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO7136412.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
BASE COUNT         1281 a          598 c          796 g         1182 t
ORIGIN      
        1 accggttaat tattttttga aatatatttt ttctggagga aaatagtaat gaataaatca
       61 ttcaatacca aattaggttt ggtaactgta gcggcattat caggtattgt attaacgtct
      121 caattacctg tgaacgctaa agctgagaca ccagtaatgg cagcaagtgc acaacaaggt
      181 ttccgttttg tagcaaccgt tgtagatagt cagacgcgtg tcttgccggg aaaattggta
      241 actttgtcag aggtgacaac tggtcagcct aaaattattg caagtgtaaa aacgaatgat
      301 gccggacaag ctatttttaa taatctccct ataaaacgta accttagtgt gtctgttgat
      361 ggacaagtaa aaggatatac tattcgaact gacgtagcag gctctagtaa agcagctagt
      421 tttacagcaa caggagtagg gacaaatgag ccaacgtatt caaaaaaaac catagacatt
      481 acagtgagag accaaaatgc tgaacctgtt tcaggcagga cagttacctt aaaaacacat
      541 gctggtcgtg agattgctag cttggtttct ggtgacaatg gattaactcg ctttactgat
      601 cgtttactag atggaacatt ctaccaatat tttgttgatg ggaaaaaatt ggtgatattg
      661 tacctggaga atcaagaagt gcatatgtta atgttgcacc taaaaaagat tattttacct
      721 ttacagtcac tgctttagat aaagatggac ttgttgttaa gggaaaagaa gtaacgttga
      781 ctgatattac tgatggtaaa gcagtagcgc ttgcttcttt aaaaacgaat gataatggac
      841 aagctatctt taccaaccta ccattatcaa gaaatatcag tgtttcaatt gatggaaaat
      901 caaagggcta tactcttcga actgatgtag caggctctca taaagcggct gcattctatg
      961 tagatggtaa aggtaccaaa gcacctcaat ttagtgcaga agcagcggtt gtaactattt
     1021 acgatgctaa tggaaatact atagcaaacc aagaagtgac attaactaat tcaaatggga
     1081 caattgttgc aaaaggtttg actgaaaaag atggcaaagc aaggttcgcc aataaattaa
     1141 tgggaggaac gttatacaat atttttgtaa atggtattga gatgcctaaa actgcccttg
     1201 ttggtagtga tgtcagtgtt tttctaacag ataaacaaat taagaaagaa aaacctgtta
     1261 ctcctgataa attggataaa gaaatgaacc aagagaagga aaaggagctc ttagatactc
     1321 caaaaccagt accacctact tcgaaaccaa cagcaccaat taaaaataaa aaggacgatt
     1381 cccctaagaa gtttacgaaa agtcaacctt ctagtttgaa gtcaagtgtt aaggctatag
     1441 gtgctaagcc aaaagctatg acagccttag ctaaaaaatt gcctaaaact ggtgatcaag
     1501 ccattagtat tttaactttg gttggtttta ttgtaacagg gttagcgatt atgttaggct
     1561 tctttaaaaa atcacgatca aaataattta ttttagaagc ttgggtcttt agacacaggc
     1621 ttctcttctt tcttcggaaa tagattgaac ttgaagaatg ttgttaaatt cgctataata
     1681 ataaagctat agacaagatt attgtttcag ttgagactac tgaaactaat actaagaaat
     1741 ggataaaagc taaggattga atatgacatt acaagacgaa atcaaaaaac gccgtacttt
     1801 tgccatcatc tctcacccgg atgctggtaa gacgactatt actgagcaat tattatattt
     1861 tggtggtgaa attagagaag cagggacagt aaaagggaaa aaatcaggta cttttgcaaa
     1921 gtccgactgg atggatattg aaaagcaacg gggtatctct gttacttcat ctgttatgca
     1981 atttgattac gcgggtaaac gtgttaatat tttggataca cctggacatg aggatttctc
     2041 ggaagataca taccggacct taatggctgt ggatgcagct gtcatggttg tagattcggc
     2101 taaaggtatt gaggctcaaa ctaaaaaact ttttgaagtt gttaagcatc gtaatattcc
     2161 ggtttttacg tttattaata aattggaccg tgatggacgt gagccactgg atttgcttga
     2221 agagttagaa gaagtactag gtattgcttc ttatccaatg aattggccaa ttggtatggg
     2281 gaaatcattt gaagggcttt atgatttaca taataaacgt ttagaactct ataaaggaga
     2341 cgagcgtttt gcaagtattg aagacggtga tcagctgttt gccaataatc cattttatga
     2401 acaagtcaaa gaagatattg aacttcttca agaagcaggt aatgactttt cagaacaagc
     2461 tattcttgat ggtgacctaa ctccagtatt ctttggctct gcccttacga actttggagt
     2521 tcagactttt cttgatactt tcttggagtt tgctccagaa cctcatggtc acaagactac
     2581 agaaggtaat gttattgacc ccttagctaa agatttttca ggttttgtct ttaaaattca
     2641 agctaacatg gacccacgtc atcgcgaccg tattgctttt gttcgtattg tttctggtga
     2701 atttgagcgc ggcatgggag ttaatttgac acgcactggt aaaggcgcta agctctccaa
     2761 tgttactcaa tttatggcag aatcacgaga aaatgtgacg aatgctgttg ctggggatat
     2821 tatcggggtt tatgatacag gaacttatca agttggggat accttaacgg ttgggaaaaa
     2881 taaatttgaa tttgaaccat taccaacttt taccccagaa ttatttatga aagtatcagc
     2941 aaaaaatgtt atgaagcaga aatcattcca caagggtatt gagcaattag tacaagaagg
     3001 agcaatccag ctttataaga attatcagac aggcgaatac atgctgggtg ctgtgggtca
     3061 acttcagttt gaagttttta aacatcgtat ggaaggtgaa tacaatgctg aagttgttat
     3121 gacaccaatg ggtaaaaaga ctgtacgttg gattaactca gacgatttag acgaacgcat
     3181 gtcctcaagc cgaaatattt tagcaaaaga tcgttttgac caaccagttt tcctatttga
     3241 aaatgatttt gcacttcgct ggtttgctga taagtatcct gatgtgaagc ttgaagaaaa
     3301 aatgtaaaat ttaaagtcac tcaaatttga gtgacttttt aagtttttta gactaaaata
     3361 gttctaatac atagaaaaga ttggagatta acatgggaat tttttcggga ttaatgggaa
     3421 atgcttcaca agtggatacc gataaagtcg agaatcagtt atctgatatt ctgattccag
     3481 gtgaacaggt tgatttagct tatactttaa ttcgtgattt gattgttttt actaattatc
     3541 gtttaatttt agtcgataaa cagggggtga caggtaaaaa ggtttcttat aattctatac
     3601 cttatgcttc tatatcacgt tttacagtgg aaactagtgg tcattttgat ttagatgctg
     3661 agttaaagat ttggatatca tcagcaatag aaccagcaga agtacttcag tttaagaacg
     3721 accgtaacat agtgagtata cagaaagctt tggcgatagc tgttttaaaa taaacaattt
     3781 agcaagtgct gctactaaga gttatttgtt ttaattataa cttttaatag tgctaaattc
     3841 tcattttcaa aataaaa
//
LOCUS       DATRSG010000102         3777 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRSG010000102 DATRSG010000000
VERSION     DATRSG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.104"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(210..2678)
                     /locus_tag="VBD89_001926"
     CDS             complement(210..2678)
                     /locus_tag="VBD89_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7136413.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(2691..3611)
                     /gene="lmb"
                     /locus_tag="VBD89_001927"
     CDS             complement(2691..3611)
                     /gene="lmb"
                     /locus_tag="VBD89_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO7136414.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT          992 a          785 c          701 g         1299 t
ORIGIN      
        1 caataaaata acacagtttt ctgtctactt atttgacatt agtccaagaa tcctcctctt
       61 tttttactat tctaacatgg ataccatttc tttgcaaaag aaaaccgcta ttatccaact
      121 tttatcacta ctcgttacat agaatgactt caaaatcacc gtaacttgct ttgacaaagc
      181 agtgctttaa caatgagatc ttcttttggt taagggttta tttgttgaag tgtcttgata
      241 tcataagtta ccaattcacc atttttatta taaaattgga caccttctgg ttggaaaatg
      301 agatacttag gatcgatatt agctttttgt gctaattgat tgatatgatc ttctagggtt
      361 gctctatcta gaccatagtc tggtaaactg tctataaagt catctgattc tttttcttct
      421 ttactggctt cacttggctg ttggttttca ttgctcttat ctgcctgttg cttttcttca
      481 ggtttatccg tatcagtagc atcaccagca tttttctttg ccaataactc ttgagcttgt
      541 tgccactcag cttgggatag atcggattta ttaatggttc tcaatgaact gccatctgct
      601 cttgggatgc taaaggagcc atctttccat acaaaagttt cttttgccaa aacatctcgt
      661 ggatcgaaaa tatagccgtc tggtgctgca aaacgacctt ctgctagggc tttttgaact
      721 tcttcagcag aatggataat ttgccagttt ggcataccag cacgtttatc aagaggcgta
      781 acatttggaa tgaccgaacc tgactcttca tgccctggct tagaccatac atccggacga
      841 acttcggggt gttgcatcac atacttgatt gttgcaatct gattgcgcgt caaccatgaa
      901 tacggaacga catggatatg atcaatatgt gggataacaa acgaacttcc agtatcgtaa
      961 gtagcattac cagcatgcat cggcagactt gacacatcta cagcaagtcg tggctcaatg
     1021 cctgtatcaa caatgtcata acggtaatgc ttcttatctt taagcattag ttcttgttcg
     1081 gcaaaggcaa tctgagtcaa atcaagttga taacgagcat agaaatagtc cttgccatct
     1141 tttggcataa tatagcccac tttaccatct tttgttactt tgcgactcac ttttttagtg
     1201 tcaaagagtg gtttttcttt gccttgttcc tgatccaaag cagcaacaag ctcatcagct
     1261 tgaccttttg ctttcaccca gttagcgacc tcatccaact catattgttc aagttctcca
     1321 aatcctatat agtggaaatg atctccgtgt ttagctgtaa ctcctgattt atccactgaa
     1381 tgaatggatt ctttactaaa aacataagca tcactcgtat catatggttt accatctaag
     1441 ccttttccgt aagctttgat gcgatgacca agaaaggtat gtgtcacttc tttatctgat
     1501 ggttttgagt gatctgaacc tgagtcgttg tcatcagttt ggccggctaa gtatcgatct
     1561 gctaattcca tttcaagagg tgataactga cttcttggga taatatgata atgatctcca
     1621 tgaggcacca catacccaaa agcgtttgat ttgatcactt gagtcggttc aaaaatcaac
     1681 ccatcttctt ccacatgacg gtatttcaaa tcaagacggt gtagttgatc taaaagttcc
     1741 ttaaaggttt ttcctttaaa ctcatctctt tggtgtttgt tttgtgacgc atcatttggc
     1801 ctaggaggcg ctggatgata accaccgtta tctggctgat gaccttgtcc agggttaggc
     1861 gtcacatcag gaattggggc tttcctacga cctggggctg gtgtcgggcg gtaatcagac
     1921 ggtctagcac ctcgaccttg tttttgactc cagtaggctt gtgcagcagc tagctcactt
     1981 ggagacaaat cttttttagg aatataatga tagtgattac catgaggtac taaataagca
     2041 tctcctaaat catcaatgat atctgtcgga ctaaaaatat agccatcgtc tgtagtatag
     2101 cgtccttgtc tttttgcttc attgactgcc gcaacttctt ctttactgag atgggccact
     2161 tgagctaaac ctttttcttt agcttcttta gttcctttgg ctacttgctc agcaatttgt
     2221 tgtttggttc gaatgttttt gcgcttacta cctggcttga ggtaaacata atagttgcca
     2281 ttgactttaa taacgtaacc gtctaagatt tcattgataa cgtctgattg tttaaaatgg
     2341 taattaggat ccgtcatcaa caactcttca ctaataatcg catcataagg aactttccca
     2401 ttgtaaaaat gataatggtc accgtgtgag gtaacataac cttggtcagt aattttgact
     2461 acgatctgtt cagcagagat gccttcttca gcactgattt gatccatcgt tttgtttgtt
     2521 ttaggggctt ttaccttacc tttgctatca tcaatatagg caatctgatt gtcctttgtt
     2581 gctagaccca tatgatgctt accaagctgg taacttccaa tatgagtagc tagtaaaata
     2641 gcagcaactg agccgatata accatatgtt ttcttcacta ataatctcct ttacttcaac
     2701 tgttgataga gcacttccaa atttgctcta agattttcta gatatgtctt gtttccgctt
     2761 ggagcagctt caagtggact taatgtcttt actttagctc ctgttgattt cgcaatagca
     2821 tgagcaattt tggggttgac gttgtcttct gcaaaaatag tcttgacgtt gtattcttta
     2881 acaaagtctt gaatttcttt caattggcga ggagagggct cttgctctgg agaaataccc
     2941 gagataccaa gttgtttcaa gccgaatcgt ttagccagat aagaaaatgc cgtgtgttgc
     3001 gtcacaaatg tttttgagcg caccttttta aatttttgag tgtattcttc agttagttgc
     3061 tctgcttctt ttttgaaagc cttagccttt ttagtgtaac tgtctttgtg tttaggatcc
     3121 aaatgtccta gctctttagc gatattaaca gcttcctcac cagctaaaac gggatccgtc
     3181 caggtatgtg ggtcataaag tgtcgcaggg tcaatgcctt gtgtgacttc catatcttct
     3241 agccctttga ctctatctag tgtcagaggt tttgacgctt caaacacatt aacctttgat
     3301 ttttttaaat tagggtctag atcccttgcc caagcttcta aggtatgtga atggtaaaca
     3361 aacaaatccg cgtcataaat agctgccaca tcatttacag acggttcaaa ggaatgaatg
     3421 cctgcacctg attggatcat cctcacatca tttagatctc cagatacttc ttttgtcatc
     3481 gcatacattg ggtaaaagct ggttacaact gacatgcctt gcgtaggctg tttggggttt
     3541 gctgacttat cacaccctgc tatcattact aaactcacaa ccatagccat gagaaaaaaa
     3601 acttttttca tagtacctcc tcaattataa tttaaccagt tattaaccag ttaattttac
     3661 tcctttatca atgctttgtc aatgacaact accatctttt atcacatcaa ctgaagggat
     3721 aagcaaaagg caattgttga cagctatttg tcctaacttc tttttgatga ggtagca
//
LOCUS       DATRSG010000103         3578 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRSG010000103 DATRSG010000000
VERSION     DATRSG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.105"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            286..1053
                     /gene="cfb"
                     /locus_tag="VBD89_001928"
     CDS             286..1053
                     /gene="cfb"
                     /locus_tag="VBD89_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO7136415.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLTNKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1226..1528
                     /locus_tag="VBD89_001929"
     CDS             1226..1528
                     /locus_tag="VBD89_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7136416.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1685..2548)
                     /locus_tag="VBD89_001930"
     CDS             complement(1685..2548)
                     /locus_tag="VBD89_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7136417.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(2853..3413)
                     /locus_tag="VBD89_001931"
     CDS             complement(2853..3413)
                     /locus_tag="VBD89_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO7136418.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHALVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVSEGFLSLEDRE
                     NVLFSDDIAEIEDFITNYKVPSTRQY"
     gene            complement(3410..>3578)
                     /locus_tag="VBD89_001932"
     CDS             complement(3410..>3578)
                     /locus_tag="VBD89_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136419.1"
                     /translation="RPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISA
                     HRTKLEKRFET"
BASE COUNT         1276 a          542 c          636 g         1124 t
ORIGIN      
        1 aaatatttag atttatataa ggtggtaaat tatcgttatg gttttttaca tgaaaatcta
       61 aattaaatta tcagaaatta acaaaaggat aggcgcaagt atattatagc aaaatgataa
      121 tattagtttt atgaaaaaaa tgcagtttat ttgtaataat gtaaaatata ggggaaaaga
      181 aagcgcttga cgaccttttg gacaagtagt aagatacaaa catgggccct gtaaattaaa
      241 aatactgcag taaaagtgat tttagtttaa aggaggaaat ttattatgaa cgttaaacat
      301 atgatgtatc tatctggaac tctagtggct ggtgcattgt tattttcacc agctgtatta
      361 gaagtacatg ctgatcaagt gacaactcca caagtggtaa atcatgtaaa tagtaataat
      421 caagcccagc aaatggctca aaagcttgat caagatagca ttcagttgag aaatatcaaa
      481 gataatgttc agggaacaga ttatgaaaaa ccggttaatg aggctattac tagcgttgaa
      541 aaattaaaga cttcattgcg tgccaaccct gagacagttt atgatttgaa ttctattggt
      601 agtcgtgtag aagccttaac agatgtgatt gaagcaatca ctttttcaac tcaacattta
      661 acaaataagg ttagtcaagc aaatattgat atgggatttg ggataactaa gctagttatt
      721 cgcattttag atccatttgc ttcagttgat tcaattaaag ctcaagttaa cgatgtaaag
      781 gcattagaac aaaaagtttt aacttatcct gatttaaaac caactgatag agctaccatc
      841 tatacaaaat caaaacttga taaggaaatc tggaatacac gctttactag agataaaaaa
      901 gtacttaacg tcaaagaatt taaagtttac aatactttaa ataaagcaat cacacatgct
      961 gttggagttc agttgaatcc aaatgttacg gtacaacaag ttgatcaaga gattgtaaca
     1021 ttacaagcag cacttcaaac agcattaaaa taatatttgt atttttcgtg tgatgctgtc
     1081 gacttcgtga ttttgtacta ccatgattgt tatgattaaa agatttacga caatagtcat
     1141 aatagtagaa cgatgtcacc attttaaata ataaagtgat tagtcatttg actaaatttg
     1201 ccaagtatca aaggaaataa agattatgac taaaaagata actgttgtag cattagaaac
     1261 attgattgcc cagcataata atatccattt gatagacgtt cgtgaagagc atgagtatcg
     1321 tggagggcat attccaggtg cgataaatct tcctttgagt caactcagtc ataagtttga
     1381 acagttagat aaaaataagg aatattatct tgtttgtcaa cgagggggaa gatctattag
     1441 agcatgccaa tttctcgagt tgaaaggcta caaagtaatc aatgtagatg gtggcacaga
     1501 agcgtggaga ggaaatctag aagtgtaaat atgtcctgtg tagtctacca tatttgataa
     1561 taaaaaaatt gacattatct cactattaat gaccttttag taaacataaa gaaaagtatg
     1621 tgttttgaaa aggaggatga ttatcctttt caaaaccata ctttttgtgt cttgtggctt
     1681 ggtcttaatt aatagttgtt aggacaatgt cgtaaatgac aaggagaata aaaggccaac
     1741 tagtaagtaa taattttgtg tgttgtttag agtttattga gtcagcctct cgaacgttag
     1801 agtgcagact tatacgacgg cgagcagtat tcctatgcgt aaaaaggtaa tataatataa
     1861 ataagattgc taaaattagc aatatatttt caaaataagt cccgtataac tcgtttactg
     1921 ttgatagctg ggtgaaaatt tctacgataa agttattgca gatatgaagg agtagggcta
     1981 ccttaaagga ataatggtaa gcgataaaac ccattatgat acccgcaata gatgtagtca
     2041 atagctgaga aatgtcttgg tgcattaaac caaataggat gccagaagtg ataatggcaa
     2101 accatttacc atacttggat aataaattca atactattcc tcggtataaa acttcttcaa
     2161 aaaaaggacc tacaacaaca gaataactaa tcataagtat actgaatgtg gcatcataat
     2221 tttgactttc cgattccata atattgagat gatttagcac agcatcccat attgtggaga
     2281 ctatattatt agtagtatat ataatgctta acaagaataa aatagtggtg aaagtgattg
     2341 tatgatgagt tgccgttaga tgttttttta aatttttaaa acccataaaa aaacaaggaa
     2401 tgagtaaggc gattaaagta cttgctatac ctatccagac atcattttgt aagatttcag
     2461 caaatagtgt agtcgccaaa cttaaaacaa tagcctgagc taaaagtatc agcccgatta
     2521 ggttaagtct ctttttttct tgattcaatt gattacctcc ttattaataa cggtaacgac
     2581 aaagaaatat tataatgtat aatgtatagg tttctaagtc attagtcaat agggacgagc
     2641 acttggatac ttctaataag ataaaataca tagacaagac tatggttttg gcttctctaa
     2701 agattaccta aaaatttata agttctcgag cagaaaggat gagtttggat attgagagga
     2761 tgggcttgac cagctgaaag aaaaatgaac caagggtaat cgcttcgaaa aaagaaggaa
     2821 atttcaatta gaaacttcct ttttttatca atctagtatt ggcgcgtgct agggacttta
     2881 taattcgtta taaaatcttc gatttcagca atatcatctg agaataggac attttctcta
     2941 tcttccagac taagaaaacc ctcactaacc atatgatcaa acatattttt taaatcatta
     3001 aagtagccat taacattata taaaatgcaa ggattatcat tttgaccgat gcgagaccaa
     3061 gagataactt cggaaatttc ttcaagggta ccaggtccac caggtagagc gataaaggca
     3121 tctccaagta gcatcatttt tgccttacgt tcaggcatat tatttactat gataagctct
     3181 gataaatttt catgtgcaat ttcacgatct cttaaaaatg tcggaataac accagtagta
     3241 tagccaccat ttgcaatcac agtatcagcc atgactccca ttaagccaac ttttcctcca
     3301 ccatacacca gagcatgttt gttttgtgcc atccattgtg ccaaggcaac tgttttttca
     3361 ctataaacag gattttcccc agtacttgct ccacaaaaaa tagttattct catgtttcaa
     3421 acctcttttc taatttagtg cgatgtgcgc tgataatctc ttctagtgag atattatact
     3481 gatcagcaag tatgaagata ttatctaaga catctcctag ttcttctttt atatcattta
     3541 agttttccaa atcagtttgt tcggtttcgt caggtctg
//
LOCUS       DATRSG010000104         3442 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRSG010000104 DATRSG010000000
VERSION     DATRSG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.106"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            151..1785
                     /locus_tag="VBD89_001933"
     CDS             151..1785
                     /locus_tag="VBD89_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7136420.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAVMDITGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLGQSEVDALVSDIKMSTVWNDSVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            1804..2076
                     /locus_tag="VBD89_001934"
     CDS             1804..2076
                     /locus_tag="VBD89_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136421.1"
                     /translation="MSKELKLPLNKMLKEQERIAIKAIVATAKSTITDTGTNLKATIE
                     TDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2086..2436
                     /locus_tag="VBD89_001935"
     CDS             2086..2436
                     /locus_tag="VBD89_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136422.1"
                     /translation="MVDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIELRRVYNDRVD
                     ELYQKAKQNEAKKG"
     gene            2884..>3442
                     /locus_tag="VBD89_001936"
     CDS             2884..>3442
                     /locus_tag="VBD89_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7136423.1"
                     /translation="MNKFFLESERLIIRPLEESDYDSWYTSFMNRGPSLTPFDDGILD
                     MSMCDINWFKNLVNLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGY
                     VFHNQFWSNGYAFESILALLNSTYEKLGFHHIEAQITPGNERSEKLVRRLGLTYETTR
                     KDFSFENGKWTDKLIYSINLHNNNLE"
BASE COUNT         1267 a          539 c          638 g          998 t
ORIGIN      
        1 aaaaatgata tcccaaatat caaatcaatg ggaatgctaa ataagccata tatacataaa
       61 aatggtattt ggcacattta tgaatagctt gaatcatcct atataaagta atagaatttt
      121 taccacaagc aaggtagagg agaagaataa ttgagtaatt ggaacttaga taattttgat
      181 aacttacata gctctttagc agaaagtgca tataatagtc gacctaattc atttcctgaa
      241 ttatttgaga ctgactcagt aacagaagta aaattttctc aaccctcaga agataacaaa
      301 ggtcaaatca cacagggtgg cactaacctc cccaatgatg gtattgtcta cctgcaacca
      361 gataagtcct taaaatctat tgatgaaaat gtaaaggtcc tcattccaga tgtcaatgga
      421 ggctatcaca ctgagcatta tgttactcat tcttatcaaa aaggtgtgct tactgacgat
      481 aaggcaggtt ttaatgctta ctacctgtca gatactgaaa aaatagatag cactacaaag
      541 cacacttatc tagccattcg tggtagtgat gggattggtc tagatacact aaacgattgg
      601 gttagtaaca atgcaatgtt tgctgtctct aacaagtata ttccacaagc gaaattggct
      661 aataaggcaa tgaaagaaaa aattgccgag ctaaaaggaa aagcacctgg agctgttatg
      721 gatattactg gtcactcact cggtacaatt gtatcatctc aagcagtagt taatcttagc
      781 tatgcagaat tggagaatgt tggtcaagta gtccttttcg atggccctga tgtatcaaga
      841 agcttggaaa aaatggaagg catttctgct aaaaaaatcc aagaagctgg gaagcatgtt
      901 acctactatg tcaatccctt tgatatagtt agtatgctta atcgtgaaaa gccatggaaa
      961 gaccaattcg ggaaagtaaa tgtcattgtt cctagtaaat atacgggtac ttttgatgag
     1021 caatcttctc acgattttgg tgccttccaa attgatagta gcggaaatcc tttagttgct
     1081 tctgaaagct atcatccaga attattaaca tctggtcaag acttagctag actcgaaaaa
     1141 aaattcataa agaatctttt aggagagcat tatgattcgg ataagctagg accagtactc
     1201 aaaggcgctc tgactgggga cctgactcga ctaattgcaa cattaaaagc ttgtggtatc
     1261 agtgtcactg taaacgaagc taaaaaactg tacgataact tccaaaaaga ctacaaagcc
     1321 ataatttcca aagctaaaaa agatggcctc gagtgggatc gcaaaaacat cgcaagcttc
     1381 catagtagga tcaaatcagc atcaggcagt caacggattt tacttagaac tcaaatgctc
     1441 tatatggcaa cgcaattagc agaagcagat gtttcagaaa aagtctctgc aacaaaaaaa
     1501 catattagtg aagctaagga tacgattgta gaaatatcga catccactat ttcatcagct
     1561 gaaataatgg ctatgcacct tggccaatca gaagttgatg ctctggtttc agatataaaa
     1621 atgtcaacag tttggaatga tagcgtagag acttctgatt atgaagcact tgatcactat
     1681 aaaacaaaaa tgaccacatt tacgaccaat ttagtaacag ttgcacaaaa tttaacagct
     1741 caagatgagc aactagctgg tgatattgtc acaaacttat cctaaaatag agagattaaa
     1801 tgaatgtcca aagaattgaa attgcccttg aataaaatgt tgaaagaaca agaaagaata
     1861 gctattaagg caatagttgc tacagcaaaa agcactatta cggatactgg aacaaattta
     1921 aaagcaacca tagagacaga tgcccaagac ctaaaaaact atgcatccac ctataacgcc
     1981 aatatcacgg atggtcttga aggccagtca gctcaagcag catctgattt cttgacacaa
     2041 cttccaaaac cagagttaga taatccagtc aactaggagg ctagtatggt agatacaaaa
     2101 aaactgcaag aactcgatca agagtatgat caaaatctca gaaacattta tcgtaacaga
     2161 gagcaactag aggatgactt tcacttgttt atggcaagaa cagatagcct aaaagagtct
     2221 gtctatcaag caactttagg tcaaggttgg gaattacccc aagaagctca tgcacaccta
     2281 tataatatgg atgataataa ggatacattc atatcagaat tcaatgaata tatggaaaag
     2341 cttgaagaaa aggaaataga gcttcgaaga gtttataatg atagagtaga tgaattgtat
     2401 cagaaggcta agcaaaacga agcaaaaaaa ggataaaaat tttttcaagt aatatatata
     2461 aatatgaaaa ataaagaatc ttgcagtaaa atgagtattt atttttcacg acgaacagtc
     2521 actacaaaat cactaaaatc tcaaaattac ttggttttta agttaatgtc taatactcaa
     2581 accttgttaa atcaatgttt gttatcttta aaaagtgctc agtagcactc aaaatccagt
     2641 gtcctcacgg acgtgtcggt tcgaccccag ccgccggtat actttaaaag ataaggtttc
     2701 tagaccttgt cttttttgtt ttgttagata atttattgtg aaaagttacc taaaattact
     2761 tatatttgtt ttttattatt gtatttttta taatgtagtt aataactttt caaggaggta
     2821 atattatgga aattttaatt aaaacacact tatttattta ctctggaaga aaggaaataa
     2881 aaattgaata aatttttttt agaatcagag agacttatta taaggccact agaagaaagt
     2941 gattatgata gttggtatac tagttttatg aatcgtggac catctttaac tccatttgat
     3001 gatgggatac ttgatatgtc tatgtgtgac attaattggt ttaaaaattt agtaaattta
     3061 caaaggaaag aatgggaaag tgacatagta tatatatttg gtgtttttct aaaaagcggt
     3121 gagcacgtag gaatgttaaa tattgttacc cttgcaagag cagatatgca atggggagaa
     3181 ttgggatatg tttttcataa tcaattttgg agtaatggct atgcatttga gtcaatcttg
     3241 gcattactta atagtactta tgaaaaattg ggttttcatc atattgaagc acagattact
     3301 cctgggaatg aacgatcgga aaagttggtt agaagattgg gattaaccta tgagacaact
     3361 cgaaaagatt tttcatttga gaatggaaaa tggacagata aactgattta tagtataaat
     3421 ttacataata ataatttaga at
//
LOCUS       DATRSG010000105         3435 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRSG010000105 DATRSG010000000
VERSION     DATRSG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.107"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            313..836
                     /locus_tag="VBD89_001937"
                     /pseudo
     CDS             313..836
                     /locus_tag="VBD89_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            907..2012
                     /locus_tag="VBD89_001938"
     CDS             join(907..1183,1183..2012)
                     /locus_tag="VBD89_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO7136424.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            2236..3368
                     /locus_tag="VBD89_001939"
                     /pseudo
     CDS             2236..3368
                     /locus_tag="VBD89_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1219 a          636 c          616 g          964 t
ORIGIN      
        1 acatcatata agatagagac tttttgtcca atgttgagcg gttaaataac tctttatgag
       61 gaatcattgc aagaactgat ttatcattct ttagtgatct tattaattct tttgaagagt
      121 tggtattatg ctttatcata ttacctaaaa agaataattt tgctgtgaca taacttgctc
      181 gagttttaaa gtcaaatgcc tctgttttaa attcttcaag tctttcagaa agatggatgt
      241 atactataaa agtagacaaa actttgtgtg ttacaatctg ttttaaaaga cacaaaaacg
      301 aaaggacaac ctatatcaac atttaaacgc tatgatgaag aattcaaaca atctcttgtt
      361 aacctttatc aaactggaaa aactcagtct gaactctgta aagactatgg ggtatccact
      421 tctacgcttg caaaatggat caaacagtat tcccaagtca aactcgaaga taattctgta
      481 cttactgcca agcaaataca agagttacaa aaacgtaatg cgcagcttga agaggagaac
      541 cttatcctaa aaaaagcaag tgccatattc atgcaaaact taaagtaaga ctcctcgctg
      601 tctatcggtt acgctttgaa cacgccacaa caaccttgtg tcgtgtttta cgtgtcaatc
      661 gctccactta ctataaattt ctaaaacata agccctcaaa aagagaattg gataatcaaa
      721 tttatagaaa acaaatactt gagatttata ccaaagcgaa caaaagactt ggtgtgaagt
      781 ctatcaaggt cattcttcaa agagactatg acacaaaaat ctctgaagga agaatttgaa
      841 taggttacac taaactagac agaaattata aagtgttcta cacttaagaa aaactggaga
      901 aaaaccatgt ctagaaaagt acgtcgtcac ttcaccgatg attttaagca acaaattgtt
      961 gacctttaca atgttggtag aaagcgtagc agtctcatca aggtatatga gctaacccct
     1021 tccaccttcg ataagtgggt tagacaagcc aaaacaactg gttcattcaa gtctgttgat
     1081 aatctgacag atgaacagcg ggagctaatt gaactcagaa aacacaataa agaactcgaa
     1141 atgcaattag atatcctaaa gcaagcagcg gtgattatgg cacaaaaagg gaaataatca
     1201 ctgctaataa ggataaatac agcatttcaa agatgtgtcg ctggctgaat atgccacact
     1261 caagttatta ctatcaagcc gtggagtcag tatctgaaac ggagtttgaa gaaactatta
     1321 aaagaatttt cctcgatagc gagtccagat acggatccag aaaaatcaaa atatgcttga
     1381 ataacgaagg tatcacactt tcacgtcgtc ggattcgacg cattatgaag cgactcaatt
     1441 tggtttctgt ttatcagaaa gccaccttca aaccacattc tagaggcaag aatgaagccc
     1501 ctattcccaa ccacttagac aggcaattta agcaagaaag accactacag gccttagtca
     1561 ctgacttaac ctatgttcgt gtaggcaatc gttgggctta tgtttgcctc atcattgacc
     1621 tatacaaccg tgaaatcatc ggcctgtctc ttggttggca caagaccgct gaactcgtta
     1681 agcaagccat acaaagcatc ccttacgccc tgaccaaagt caagatgttc cattcagatc
     1741 gtggcaaaga gtttgataat cagttaattg atgaaatatt ggaagccttt ggaatcacac
     1801 gttcgcttag tcaggctggt tgtccttatg acaatgccgt agctgaaagt acgtatcgtg
     1861 ctttcaaaat tgaatttgtt tatcaagaaa cctttcaatt gctggaagaa ctagctctta
     1921 agactaaaga ctatgttcat tggtggaatt accaccgcat tcatggtagt ctcaactacc
     1981 aaacaccaat gactaaaaga ttaattgcct agaaaaacac tttataaatg ttgtacagaa
     2041 aagtgttgcc ttttcactta ataaaagaaa gattgaagga acacttctct ttcattccga
     2101 ccaagggtca caatttaagt ccgggaaatt tagaaaaata attgatgaca acaataggca
     2161 ctgaatccct aaaatgagac aaaagaaaaa cactcctgtt gaaatttagt aaactagaaa
     2221 caggagtgtt ttattatggt taaaaaagca tattcatggg aaactaagct ggcttgcatc
     2281 gatatgaaga aagcaggcaa gtctaacaga gttattatgg agactttagg catcaaaaat
     2341 aatagtcaaa tctatacttg gatgaaatgg tacgaaaacg aggagcttta tcgttttcat
     2401 caaggtgttg gcaagcaata tacctacggt aaaggcttgg aacatctgtc tgaagtggaa
     2461 caattacaat tacaggttga cctcttaaaa aagtatcgag gcttgataag gaaatcgata
     2521 aagtaagtct tatcaagctt gtggaagact ataaaaacaa ctacccgata gttttcattc
     2581 tagactgttt taaggtcaag cggtcgacct attaccgttg gaaaaaggag tatgagaagc
     2641 cgcaaaaaaa gatgacctta ttgaattaat tgaacagctc tgtatggaga atcactttat
     2701 ttatggctat cgtaccatta cacgattgct caaaaaaatc cacggattga ctgttaatac
     2761 aaagaaagtc tatcgtatca tgaaaaataa tggctggctt tgtcggacac gtaccaagaa
     2821 agttcccaat ttgggtaaag cttattattt aacagataat aaattgagtc gggatttcca
     2881 tgccgataag cctaaggaaa agctcgtaac agatattacc tacctatatt tcggtaactg
     2941 taaattgtat ctctcatcaa ttatggatct ctataaccga gaaattatag cttacactat
     3001 ctcagattgt caggacacgg attttgtact agacaccctt aatcagttaa aattgcctaa
     3061 ataaaggagc aatcttacac agtgaccaag gctcagtgta tacttctatg gcttactacc
     3121 aggcttgcac ggaaaaagac attatccgct caatgtctcg aaaaggaaca cctgcagata
     3181 atgcctgtat tgaatggttt cacaccgtcc taaagactga aaccttttat ttccataata
     3241 ggagaaaata caacaaagat agtataacaa atattgttaa aaattacatt acattttata
     3301 atgaaactag aattcaacag agattaaacg accagtcgcc tgtacagtac agaaaactga
     3361 ttgcctaaaa gtgtttttct tgcgtctcac tattagggtg cagtgccaat atcatgcatt
     3421 ctttttctaa tcctg
//
LOCUS       DATRSG010000106         3343 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRSG010000106 DATRSG010000000
VERSION     DATRSG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.108"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..162
                     /locus_tag="VBD89_001940"
     CDS             <1..162
                     /locus_tag="VBD89_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO7136425.1"
                     /translation="KSNFNQKERVTFINYHSKALSYFIKYELINNSKEELFNRKVIEK
                     IIRTSIENY"
     gene            172..1158
                     /gene="dhaQ"
                     /locus_tag="VBD89_001941"
     CDS             172..1158
                     /gene="dhaQ"
                     /locus_tag="VBD89_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO7136426.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
     gene            1413..1688
                     /locus_tag="VBD89_001942"
     CDS             1413..1688
                     /locus_tag="VBD89_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136427.1"
                     /translation="MKKNSLYNQLINSEPIGFIDPLTDLGEFDIFQMKFKEPVRYLKN
                     KYSGKPYSPHWQNKIEKMRSHQESLREEVKEEIPITSRARNQENNLS"
     gene            1830..2108
                     /locus_tag="VBD89_001943"
     CDS             1830..2108
                     /locus_tag="VBD89_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7136428.1"
                     /translation="MTGTQNLNANFEDEKSSMVYYENMVAHYIRLTNHHVRYRVTLLF
                     KNIEMVTRGVGMETQSVEDNQISFDIYIFNVQSGYNINYLTRYSVKEN"
     gene            2118..2318
                     /locus_tag="VBD89_001944"
     CDS             2118..2318
                     /locus_tag="VBD89_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136429.1"
                     /translation="MNSEIRNRKHVYLNDDIIFQLAEISEFLKSSQSTFKQERSPYSQ
                     IIDLRKEEAQAKSVRNSIEKLL"
     gene            2449..>3343
                     /locus_tag="VBD89_001945"
     CDS             2449..>3343
                     /locus_tag="VBD89_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEO7136430.1"
                     /translation="MKICILGLGVIGTTYGYIFQKAGHQVEHLVRESKKQDVPLKLDI
                     TMLDGRYNNKGEEKTDIYTVNLAKPNTNYDFILLSVAGGRIKDAIATLSQNNITGSLL
                     LFCNFWNSREEIEKMVGDFKYIIGFPTAGGNLKQKKLNCVLFDHIMLESEEKATITNY
                     KELLEFLESADIKVENPYDMIEWIWIHMAINAGVTSTAARAGKIDDPKQLAVNLMRDN
                     HGLSIAVKSIRETIKVVKNRGVDLRLYKNELFPYKIPSTIAGIIMKRMFARNELTSRI
                     MTLHNDVKDIMYGCKCIYDAGK"
BASE COUNT         1284 a          421 c          637 g         1001 t
ORIGIN      
        1 aaatctaact ttaatcagaa agaaagagtt acatttatta attatcatag taaagccctc
       61 tcttatttta taaaatatga gttaataaat aactcaaaag aagaattatt taataggaaa
      121 gtcattgaaa aaattatcag aacatcaata gaaaattatt aggacaagga tatgagaaaa
      181 acagaacatc ataaaatgga atatgttttt gaaacaataa aagcaatcac taaaactaat
      241 cctaaagtag agcaaataaa agatttgccc gctattcatt atttagggca atccgagcaa
      301 gtaattccga ttttatcagg aggaggcagc ggacatgagc cggctcactt tggatatgtt
      361 ggtgagggaa tgctatcggc agcgattagt ggtcctattt ttgtaccacc atgtgctagt
      421 gatattctag agactattag atttatcaat cgaggcaaag gtgtctttgt tattatcaaa
      481 aattttgaag ctgatttgga agaattttcc caagcgattg aacaggctcg tcaagaaggc
      541 attccaatca aatacatcgt ttctcatgat gacatctctg ttgaaacatc aaattttaaa
      601 attcgtcatc gaggcgttgc tggtactgtg ttgctacata aaattattgg acaagctgct
      661 ttagaaggag caagcttaga tgaattagaa cagctaggac tatctttgac aacttcaatg
      721 gctactttag gtgtagcaag taagtcagcc actattttag gtcagcatca acctgttttt
      781 gatttagaag aaggttacat ctcatttgga atcggtattc atggtgagcc aggataccgg
      841 acaatgcctt tcgtgtccat ggaacatttg gcaaacgaac tagttaacaa attgaaaatg
      901 aaattacgtt ggcaagatgg agaagcattc attctattga taaataatct tggaggaagt
      961 tctaaaatgg aggaattact ttttacaaat gctgtcatgg aatttctggc tttagacgat
     1021 ttacaattac cttttattaa aacaggccac ctaattacaa gtttagatat ggctggttta
     1081 tcagttacct tgtgtcgtgt aaaggatagt agatggattg attacttaaa acataagaca
     1141 gatgctagag cttggtgata gaagtaagtg gtaagagttt ttagaataga aaatacttta
     1201 aggagaacag agaaatctgt tctctttttg ttcgcttaaa gatcatagaa aggtgaagag
     1261 aacatggaag gtattgaaat aatattagat atagaaacgt taggaatttt tcaagtcata
     1321 gatcatggta tctctccaga tgatagagaa acatatgaac aattaacttt gttttaaata
     1381 acttaacttt attatcttag aggatgttac atatgaaaaa gaatagttta tataatcagt
     1441 taataaatag tgaaccgata ggctttattg atccactaac cgacttgggg gaatttgata
     1501 tttttcaaat gaagttcaaa gaacccgtta gatacttgaa aaataaatat tcaggaaagc
     1561 cttattcacc acattggcag aataaaattg aaaaaatgag gtctcatcaa gaaagcctaa
     1621 gagaagaagt taaagaagag attccgatta ctagtagggc aagaaatcaa gaaaataatt
     1681 tgagttgatc ttaaattgat aaaaagttcg acttttttcc taaaaaatag tgtgctataa
     1741 gtcaattaaa agatgtgcta tatttattta atagatatcg tctcattgaa tatcaactaa
     1801 ccggagaaaa ttcgaatgca aaaaatttaa tgacaggcac tcaaaattta aatgctaatt
     1861 ttgaagatga aaaatcgtcg atggtatatt atgaaaatat ggtggctcat tatataagac
     1921 taacaaacca tcatgtgaga taccgtgtta cactcttatt taaaaatatt gagatggtta
     1981 caagaggggt tggtatggaa acccaaagtg tggaagataa tcagatatct tttgatattt
     2041 atatttttaa tgtgcagtca ggatacaaca ttaattattt aacaagatat tccgttaagg
     2101 aaaattaaag gagaaaaatg aattcagaaa ttcgaaatag aaaacatgtt tatttgaatg
     2161 atgatattat ctttcagtta gctgaaatct cagaattttt aaagtcaagt cagtcaacat
     2221 ttaaacagga gcgtagtcca tattctcaaa taatagacct acgaaaagaa gaagcacaag
     2281 ctaaaagtgt taggaatagc atagaaaaat tattgtaaga aaacagtgat ctaatacagg
     2341 taaggaagta tgagttaggc tataaaacta agtaaaaaaa tatgatataa tataaaaaat
     2401 tatcgagtaa aataaactgg aagcgaaact taaggagatg attattaaat gaaaatatgt
     2461 attttaggat taggcgtaat aggcacaact tatggatata tctttcaaaa agcaggacat
     2521 caggttgaac atttagttag agaaagtaaa aagcaagatg ttccattaaa gctggatata
     2581 accatgttag atgggcgata taacaataag ggagaagaaa aaacagatat atatactgtg
     2641 aatcttgcaa aaccaaatac aaattatgac tttatccttt taagtgttgc aggcgggagg
     2701 ataaaagatg ccatagcaac actttcacaa aataatatta caggaagttt gttattattt
     2761 tgtaactttt ggaacagccg tgaagaaatc gaaaaaatgg ttggtgattt taaatatatc
     2821 attggatttc cgacagcagg tgggaatttg aagcagaaaa agttgaattg tgttctgttt
     2881 gaccatataa tgcttgaaag tgaagaaaaa gcaactatca caaactataa agagttgtta
     2941 gaatttctcg aatctgccga cataaaagta gaaaatcctt atgatatgat agagtggata
     3001 tggattcata tggcaataaa tgcaggtgtt acttctactg cagcaagggc aggaaaaatt
     3061 gatgatccaa aacaacttgc ggttaatctt atgagagata atcatggatt gtctatcgcg
     3121 gtaaaatcaa taagagaaac aattaaagta gtaaaaaata gaggggtcga tttaagatta
     3181 tataaaaatg aactttttcc ttataaaata ccttctacta ttgcaggaat aattatgaaa
     3241 cgaatgttcg caagaaatga acttacgagt agaattatga cgttgcacaa tgatgtaaaa
     3301 gatattatgt atggttgtaa atgtatctat gatgctggaa aac
//
LOCUS       DATRSG010000107         3246 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRSG010000107 DATRSG010000000
VERSION     DATRSG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.109"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            212..1288
                     /locus_tag="VBD89_001946"
     CDS             212..1288
                     /locus_tag="VBD89_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7136431.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQIIQKLNDLKKDY"
     gene            1290..1496
                     /locus_tag="VBD89_001947"
     CDS             1290..1496
                     /locus_tag="VBD89_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO7136432.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGPIAFLILEGNNE"
     gene            1493..2395
                     /locus_tag="VBD89_001948"
     CDS             1493..2395
                     /locus_tag="VBD89_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136433.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            2392..3153
                     /locus_tag="VBD89_001949"
     CDS             2392..3153
                     /locus_tag="VBD89_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136434.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPETTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGILLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
BASE COUNT         1128 a          451 c          552 g         1115 t
ORIGIN      
        1 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
       61 atttaaccat tgttattatt aatgctgttt tgccacttat taatcaacga aaaagtttct
      121 tctatggaaa cttttttata ttatatttcc tgataggaac ttttattttt gatttagaaa
      181 ctctataatg gacgtgaaag gagattgacc gatgcttgga gagaatatct atttacaacg
      241 tactcagatt ggtatgacgc aagagaattt gagtgattat ttgcatttaa caaaaactac
      301 aatttcaaag tgggaaaata atcaagctaa accggatatt gactatctta ttttgatggc
      361 taatcttttt gatattagct tggatgactt agtcggttat caaaaaacat tgtcagatga
      421 tcaaagaaat cagctcatta aggatctaaa aataaaagca aatgtattgt ctgagagaga
      481 tttctttcag gaagttaagg aattatcaaa acagttccct aacgatttta agactctgtt
      541 gataatgatt aatatggtct tgagcaacct aaccaacctt aatgattcag aaatgaaaga
      601 gtggtcacta agtacactaa ataaaattat tagtaaaaca gtagttgagt ctgacctcca
      661 gacggcgata atgttcaaaa cggtcgttct ttttcacaac cttgaatatg atgaagtgat
      721 aaacctttac cagaatagtc cctataaatt aggtgaagaa ttacttttag ctaatagctt
      781 ttttgcaaaa ggcgatgttt accgagctaa gcaagtatta caggttgaaa tttatcagca
      841 gattctactt gtatgcgagt atctattgag tttatcaact tttgaagttg aagaaaacaa
      901 aggcgatatt tttgaaaggc ttgaacatat gatagaagcg tttcaattaa actctttgca
      961 tcccaacaca gtcatcaagt gttattatag tttagcttgt cattatagta aaagtgatga
     1021 tgataaggca ttaggatatt taaaacaatg ttttaaatct ttaaaacagt tgctccaaag
     1081 atttgaatta cacggtgata ctttctttta taccatcgat gattggttag agacaatacc
     1141 tactggtatt gcaccaccga cgagtcaatt acaggtaata gaacgagttg aggacttatt
     1201 tcaaaattca caatttaaag aactatcagg gcgtaaagaa tttcaacaaa ttattcaaaa
     1261 acttaacgat ttaaaaaagg attactaata tgaatacaga aataccaaat caattattac
     1321 cattcctagt tccgttgatt atgttgcaag gagctttaat tatcatttct ctagtcaaac
     1381 taagtaaact taagatgact aaacaccttt caaagccagt ttggtttttg gtcattatat
     1441 ttcttaatat aattgggcca attgcatttc ttattttgga agggaataat gaatgaaagc
     1501 tatctcattt caaaacgtta caaaatcttt tggtcctaaa aaaatattaa ataatgtttc
     1561 ttttgattta gaagaaaata tgatttatgg attcgtagga ccaaatggag caggtaaaac
     1621 aacgacaata aaaatgattt taggattgtt aaaatttgat acgggtttca taacaatttt
     1681 tggtaaaaaa gttaattttg gaagaacaga taccaatcaa ctgattggct atctcccaga
     1741 tgttcccgag tactacgatt atatgacagc tctagaatat cttgatttat gttctggttt
     1801 agcgagatca aagcataagc tttctaacaa agaactactt aggtcagttg gacttgacga
     1861 taatcaccaa aagattgcta cttattctcg aggaatgaaa caacgtcttg gattagccca
     1921 agcactggtc catgatccta aaattattat ttgcgatgaa ccaacctctg cattagaccc
     1981 gaaaggtcgc caagatattt tagatattat ttcaaatctt agaggagaaa aaactgtgat
     2041 tttttctacc catattctct ctgatgtcga aaaaatatgt gatcacgttc tggtgttaac
     2101 taagtgtggg atttattctc ttgaagagtt aaaaggaaaa aaatcagagg aaaattattc
     2161 ggttcgtatt ttaataaaag tgacaaagtc tgaagctaag gtactatctc ataactatca
     2221 gatagaaaag aaagataatg agtatgcact gactctcaaa ggctctaaaa tggataacaa
     2281 agcagattta ctagctggtt tttaccaaga tttagtttct ttaaagatta gcccatcagc
     2341 tattgaagtc attgacaata gtttagagga gctttatttg gaggttacca aatgaaatct
     2401 ttagcagtaa tgttaaaaaa agaatggatg gagaatgtta ggacatacaa agttatatct
     2461 attctgatta cttgttcaat atttggtata ttaggtccct taactgcact tatgatgcct
     2521 gatattatgg caggaatttt acctaaaaaa ctacagggag cgataccaga gactacctat
     2581 attgattctt atattcagta ttttaaaaat atgaatcagc ttggtttagt tattttagta
     2641 tttttattta gtagtacact gacgcaagaa ttttcaaaag gaaccttaat taatctagtt
     2701 acgaagggat tagccaaaaa agttataatc cttgctaaat ttatagtaat tacccttcta
     2761 tggacagtgt catacttatt aagtgttgtc atccatttta gttatacgtt atattacttt
     2821 agtaacgaag gaagtcataa attaatggtt tacggtgcaa catggtttat cggtattctt
     2881 ttcataagct taatactctt tttttcagtt ctttttcgta aaactttagg gattttgtta
     2941 ggttgtcttt ttatggtggt gttatgtgta gtatctggat tttttaaaat cagtaaaagc
     3001 tataatccta tgcttcttat ccaaaaacaa ggagaaatgt taagtggaaa gtttaaagtt
     3061 caagacttaa tggaaccgag catattagtg tttttgctta ttataatgac gttattactt
     3121 agcatgtaca tttttgaaaa aagtgaagtt taattttgat aagatatcaa taaggcgata
     3181 gctacttttt gaaaaaataa aaccgaatta agagctttgc tgctatttga cttatctaga
     3241 tcaata
//
LOCUS       DATRSG010000108         3213 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRSG010000108 DATRSG010000000
VERSION     DATRSG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.110"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1256)
                     /locus_tag="VBD89_001950"
     CDS             complement(<1..1256)
                     /locus_tag="VBD89_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO7136435.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNG"
     gene            complement(1256..1837)
                     /locus_tag="VBD89_001951"
     CDS             complement(1256..1837)
                     /locus_tag="VBD89_001951"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO7136436.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAEAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(1941..2036)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2092..3075)
                     /gene="guaC"
                     /locus_tag="VBD89_001952"
     CDS             complement(2092..3075)
                     /gene="guaC"
                     /locus_tag="VBD89_001952"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO7136437.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
BASE COUNT         1106 a          659 c          475 g          973 t
ORIGIN      
        1 aatccattca aaatgatatt taaaacaaca gctgtcaggg ttgagataac aataccattt
       61 gtcaaaaaca tttgtaaagt attaggaaga ctaatgaaga gatttgtctc attaaaacct
      121 actcctgctg caattgatac ggctgcaatg ataaagttat gctcattatg ctcaaaatca
      181 acctgattaa gcattttcat cccttgaagt gctaccatac caaataaaac taacatagca
      241 ccaccaagaa ctggacttgg aatcatttga gccattgcgc caaatttagg taacaagcca
      301 agtataacta agaagagagc tgtaaaatat atcggcttac gcgtacgtat cccagatatc
      361 tgtaccagtc ccacattttg agaaaaacca gtatatggaa aggtattaaa taagccgcta
      421 agtaatactg ccaatccttc tgaacggtaa ccattacgaa gtctcttact gtctaattta
      481 tcgtttgtaa tatctgaaag cgcaaggtaa acacctgttg attctaccat agaaactgtt
      541 gcaataatac acatcattaa aatagaagta aattcaaatc taggagctcc aaaataaaat
      601 ggcttcggaa tatgtacaag tggtgcttct gctaccacag aagcatcaac taagcccata
      661 aatgctgcaa gaattgtacc tgaaatgagt ccaataagga ttgaaattga cttcaaaaag
      721 ccttttgcga aaatattaat taataaaaca acaccaatcg ttaccagtga taaagttaaa
      781 gattgtaagc ttggttcttt tgcattatca cccatatttc ccatcgcaac tggtattaat
      841 gtcaaaccaa ttgtagtaat aactgatcct gttacaattg gaggaaagaa atttgcaact
      901 ttagaaaaaa tgccagcaac taaaacgaca tatattcctg aagcaattaa agctccaaac
      961 atatagccag aaccttgttg tgcaccaata attgataaag gagctacaga ctgaaaggca
     1021 caccctaata caactggaag acccactcca aagtgcttac ttaatcgtaa ttgtaataag
     1081 gtggcaatcc cacacataaa aatatctgtc gcaataagat aagttagttg cttagcatta
     1141 taaccaagag cactagcaat catgataggt acaagaatgg atcctgcata catagctaac
     1201 aggtgttgca agcctagaag tgctgcttgc gaattacttt gctcattaaa tttcattaag
     1261 catcagcctc cgcaaaaaca actcgaccat tttcaaacgc tttaatacga gcaagagaag
     1321 taaccggaac acctgttttt tctaacaaat cacgcccatc ttggaaagat ttttcaataa
     1381 cgattccgat accagcaacc ttagcttcag cttgaccaat aatttcaagt aatcctttag
     1441 ccgcttgacc gtttgctaaa aagtcatcaa tgatgagtac agtatcatcg ttagataaaa
     1501 agcgactcac aatagaaact tgactcgtaa cttgctttgt aaaagaatac acttcagcag
     1561 ttaagatacc ttcagtcata gtaatgttct tagctttttt agcaaatatc attggtacgc
     1621 ccaatgcttg agctgcgtac actgctggcg caattccaga tgcttcaatt gtaacaacct
     1681 tcgtaatgcc ggcttcttta tatttatcag caaaaacttt acctatttcc tgcattaact
     1741 caaaatttac ctgatgagtc aaaaaagaat caacttttaa aatgttctca cctaaaacat
     1801 caccatcttt aagaatacgt tcttccaata acttcataat aaccccttca ctctttagaa
     1861 aataaaaaaa taacttacct caatcagagg acacaaatct cctctaattt atcgcttcat
     1921 taacatgaac caacaggcaa gctatttatt agcttactta ttgttagaca ttacggtgtc
     1981 ttgtagagac gctaagccaa tcctcagcat aaataagtaa atcatatatt taattgttat
     2041 gtatgataga taactattgt aacattttta attaagattt caagatttaa tttaaatgga
     2101 atcaccattc caaatagaat ttttaacaat aacgtaatca acgtgtctca agctatccaa
     2161 ttcttttcca cctgcgtaag aaatagaact ttgcaaatct tgttgcattt ctgtcaaagt
     2221 atcctctagt cgccctttaa caggtaatag aattttctta ccttcaacat ttttatgttc
     2281 tcccttttga tattcagaag ctgagccata gtattcttta aattgctgtc cttctacttc
     2341 cacaagtttt ccaggacttt caaggtgacc agcaaacagc gaaccaatca ttaccatact
     2401 agcaccaaag cggatagatt ttgcaatatc cccatgtgtt cgaataccac catcagcaat
     2461 aattggcttg cgagcagctt tagaacacca acgtaaggca gctaattgcc aacctccagt
     2521 accaaaacct gtcttgactt tagtaataca aactttacca ggtccaatac caactttagt
     2581 tgcatctgca cctgcatttt ccaattctct aacagcttca ggcgttccta catttccagc
     2641 aataacgaaa gttcctggaa gctcttgttt aatgtgttgg atcatctcta taacactgtt
     2701 agaatggcca tgtgcaatat caatagtgat aaactcagga gcatcttctt tcagacttgt
     2761 cacaaaatca tattcatagt ctttgacccc aaccgaaatc gaagcaatta agcctttatc
     2821 atgcatacgc ttgataaaag gtcttcttgc ttcttcatta aaacggtgca taatataaaa
     2881 ataaccttca cacgctaatg tttcagctac ctcttcatca ataatagtct gcatattagc
     2941 agggattact ggaagtttaa aggtatagtt tccaagtttg acacttgtgt cagcttgtga
     3001 gcggctagat ataatacact tgtttggtat taattgaata tcttcataat caaaaacagg
     3061 aatatcgtta aacataatgg ttctcctaat gagtaaaatg accttcctat aactttgtat
     3121 aacaaagcat cgctaggtaa aaacatacct ttccttttta aattatatac tttatttatt
     3181 tttagtcaat ttttgatcaa tttatttgaa taa
//
LOCUS       DATRSG010000109         3088 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRSG010000109 DATRSG010000000
VERSION     DATRSG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.111"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..124
                     /locus_tag="VBD89_001953"
     CDS             <1..124
                     /locus_tag="VBD89_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7136438.1"
                     /translation="SNNTLDIWIRKGMPHIRIGKTIRFDKDSIRRWMIQLENHF"
     gene            179..1402
                     /locus_tag="VBD89_001954"
     CDS             179..1402
                     /locus_tag="VBD89_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7136439.1"
                     /translation="MTITKTSNGSYRLKLYVPTEARPSLGISGNYYDKRFKTKKEAKE
                     AELQILIDINTIQKGGALPHQAINGDILFKDFYHNVWWEAYKAGQTTSTNKPPTLPTV
                     NNTETVFRLHILPMFGNYSIKFLNQNKTVVLNLMTVKANEYANFKVIRSYVNSIFDWA
                     EELEYIEMNRLSKTISRIKATKKIKLQEAKRDEDLYLTLSELREWFEMFQEDLENKKL
                     SLKDYVLFFTTFILNDRKSESYALQWKHISLDTSEIGLIQALDKYKKPKSTKGEKKTI
                     FAIPQELTVLLYQWKIQQKSELAKFGIIQTPEQYVFTYIDTKGNVNSPLHADYLNNKM
                     KSVERRHKNLRHATPHMLRHTGATLAKKAGMSLESISEALTHSDLSTTKIYVNTSNVV
                     PLSAGETAYRELKNK"
     gene            complement(1508..1900)
                     /gene="rpsI"
                     /locus_tag="VBD89_001955"
     CDS             complement(1508..1900)
                     /gene="rpsI"
                     /locus_tag="VBD89_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO7136440.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1921..2367)
                     /gene="rplM"
                     /locus_tag="VBD89_001956"
     CDS             complement(1921..2367)
                     /gene="rplM"
                     /locus_tag="VBD89_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO7136441.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(2589..2711)
                     /locus_tag="VBD89_001957"
     CDS             complement(2589..2711)
                     /locus_tag="VBD89_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7136442.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
BASE COUNT         1042 a          555 c          528 g          963 t
ORIGIN      
        1 ttcaaataac acgctggata tctggataag aaagggaatg ccacatattc ggattggaaa
       61 aacaattcga tttgataaag acagtatccg tagatggatg atacaactgg aaaatcactt
      121 ttgatttcat tataataaag tcagggtaat tttctagctc agtaaaaagg agattactat
      181 gacaattaca aaaacctcaa atggttctta tcgtttaaaa ctctatgttc caacagaagc
      241 tagaccttct ttgggaattt caggaaacta ctacgataag cgtttcaaaa caaagaagga
      301 ggctaaagaa gctgaactac aaattttaat agatattaac actatccaaa aagggggtgc
      361 attaccacat caagcaatca atggtgatat tctcttcaaa gatttttatc ataatgtctg
      421 gtgggaagcc tataaagcag gtcaaacgac ttcaacaaat aaaccaccca ctcttccaac
      481 agttaacaat acagaaactg tttttagatt acatatttta ccaatgtttg gaaattattc
      541 cattaaattt ttaaatcaga ataaaacggt tgttttaaat ttgatgactg ttaaagcaaa
      601 tgaatatgcc aatttcaagg tcatacgtag ttatgttaat tctatttttg actgggcaga
      661 ggaattagaa tatattgaaa tgaatcgcct atctaaaaca atcagtcgta ttaaagcgac
      721 taaaaaaatc aaattacaag aagctaagcg tgatgaagat ttatacctta ctttaagtga
      781 gttacgggaa tggtttgaaa tgttccaaga agacctagaa aacaaaaaat tgtcgcttaa
      841 agactatgtt ttatttttta caacttttat cttaaacgac cgtaaatcgg aaagctatgc
      901 tttacagtgg aaacatatct ccttagatac atctgaaatt ggtttaattc aagccttgga
      961 taaatataag aagcctaagt ctactaaagg agaaaagaaa acaatttttg ctattcctca
     1021 agaactaact gttctcttat atcaatggaa aattcaacaa aaatcagaat tagcaaaatt
     1081 tggtatcatt caaactccag aacaatatgt atttacttat attgacacta aaggcaatgt
     1141 gaatagccct ctgcatgcag attaccttaa taacaagatg aaatctgtag aacgtcgcca
     1201 taaaaatctt aggcatgcca ctcctcacat gttaaggcat actggtgcta ctttagctaa
     1261 gaaggcagga atgtctctgg aatccatttc agaagcttta acacatagtg atttgagtac
     1321 aacaaagatt tatgttaata cttcaaatgt tgttccactt agtgctggcg aaactgctta
     1381 cagagagctc aaaaataaat aaggtaaaaa taaggtgaat tttgaggtga gtttttcaaa
     1441 aagtgcacga aaaaacaccc aatgaggtaa acattgagtg tctgtaatcg ttgctgtaaa
     1501 gctgttctta acgttttgag aactggctag ctttacgtgc tttcttaaga cctggttttt
     1561 tacgttcaac catacgtgcg tcacgtgtaa gaagtccagc acgtttcaat gaatcgcgga
     1621 aatctgggtc aacttctaaa agtgcacgtg agataccatg acggatcgca cctgattgac
     1681 ctgcatatcc accacctaca acgttaacaa aaacatcgta tgaaccttga gttgaagtaa
     1741 ctgcgaaagg ttggttgata acaagacgaa ggtctgcgtg tgggatgtac tcttctacat
     1801 cttttttatt gattgtaatt ttaccagtac ctggaaccaa acgaacgcgt gcaacagcgt
     1861 ttttacggcg accagtacct gcatattgtg cttgtgccat tatataatgc tcctttcctc
     1921 ttagataagt cctgagatat caagtacttc tggttgttgt gcagcatgtg tatgctcacc
     1981 gccaacaaat actttcaatt tcataccttg tgcacgtcca agagtgttat gtggaagcat
     2041 gcctttaact gatttttcaa tcaaacgaac agcattttta gaacgaagtt cacctgctga
     2101 gatttgtttc aaaccacctg gatacattga gtgagtgtag tagattttat ctgatgcttt
     2161 tttaccagtt aatttaactt tttcagcatt gataacaatc acaaagtcac ctgtatcagt
     2221 gtgtggtgtg aaagttggtt tgttttttcc gcgaagtacg ctagcaacta ctgcagaaag
     2281 acgtccaaga ggtacatctg ttgcgtcaac aacgtaccat ttgcgttcaa cttggcctgg
     2341 tttagccatg aaagttgttt tgttcatgat ttctcctata cgaattcgtt tttgtttaca
     2401 ggtgtggatg ttccggtcca cgagagtatt tgaaaggttc cggggcctta caaatggggt
     2461 aaacaatacc gcttataatc atatcaaaat aaggtagcat tcgtcaagtt atttctttta
     2521 ttttatcaat tttcatccca aaataaatca tctacagttt tattcaaagc ttgacacaat
     2581 ttaatcgctt aattatagcc tcctttttca atggcggcta ttgtctgaca tgatacaccg
     2641 accttttcag ctagagactg ttgtaataaa tccatactag ctctagctgt ctttaagcgt
     2701 aaatttttca tattactatc tgtccttata cttaaggtag tatactgaag ttcccatagc
     2761 tatcaatgat attcctataa ctgctaaaat tgcatcattt ccccttgaaa aataagtagc
     2821 aatatcaact tcctagtaaa agcatcaata aaatagttat tcctatgagg gcaaatgcgc
     2881 cagcaacaaa aacattttcc aaaatctttc atcaacaaac ggagagactc tttttagatt
     2941 agaagtcgta acaaaataga aataccacta aataaagtta atattattat aaaatcaggt
     3001 gacacgcttc cctttattag ctttccaaaa acactttcaa gccataagat aaaaaacatc
     3061 gtctaaaaac atttttgata actttgta
//
LOCUS       DATRSG010000110         3043 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRSG010000110 DATRSG010000000
VERSION     DATRSG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.112"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(257..865)
                     /locus_tag="VBD89_001958"
     CDS             complement(257..865)
                     /locus_tag="VBD89_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7136443.1"
                     /translation="MKNNIIFSIEQYINDQEMPKGKQKVILSAIELFASQGFHGTSTA
                     QLAKNAEVSQATIYKYFETKDKLLVFILELIVQTIGRPFFTELSTFSTKEELIHFFVQ
                     DRFKFIEKNNDLIKILMQELLINSETSTIFTKLINSTDPNITKIFNCLSEGNSLNKME
                     ILRAVIGQFITFFIQLYILNIKPENLEEELKQIEKQILKLLS"
     gene            complement(889..1986)
                     /locus_tag="VBD89_001959"
     CDS             complement(889..1986)
                     /locus_tag="VBD89_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136444.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTIVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSSNLLVLLLFLIILTIANIFGLKRYRKV"
     gene            complement(1989..2705)
                     /locus_tag="VBD89_001960"
     CDS             complement(1989..2705)
                     /locus_tag="VBD89_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7136445.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALHESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT         1027 a          436 c          494 g         1086 t
ORIGIN      
        1 ttgggatgtt actagtaggc tgtcaaatga ataggggacc taaaagtcag tcaaacgaag
       61 taaaaaatag caagcaatca gaagtgaaga aaggtaaaaa tatgataaaa aagaacagtt
      121 agcctactta aagaacatga acaagaaata atggtgaatt atttaaatgt atcttttgat
      181 agagtagagt atacgatggt atttttaatg agatgtagag tagattgaat gctttcttta
      241 aatttagaag tattatctag ctcagtaatt ttaatatttg tttttctatt tgttttagtt
      301 cttcctctag attttcaggc tttatattaa ggatatataa ttgaataaag aaagtaatga
      361 actgtccgat tactgctcga agtatttcca ttttattcaa cgaatttcct tcacttaaac
      421 agttgaaaat tttagtaatg tttggatcag ttgaatttat tagcttagta aaaatagttg
      481 atgtttcact attgattaat aattcttgca tcaatatttt aatgagatca ttattttttt
      541 caataaattt aaaacggtcc tgtacaaaga aatgaattag ttcctcttta gtagaaaatg
      601 tagataattc tgtaaagaag gggcgtccaa tagtttgtac gattaactcg agaataaaga
      661 ccaaaagctt gtctttagtt tcaaaatatt tatatatagt tgcttggcta acttctgcat
      721 ttttagctag ttgagcagtg gaagttccat ggaagccttg tgaggcaaag agctctatag
      781 cggataaaat aactttttgt tttccttttg gcatctcttg gtcattaata tactgttcaa
      841 tagaaaaaat aatattattt ttcatactta gttccttttc aaatggtatt atactttacg
      901 gtatcgtttt agtccaaaga tatttgcgat ggttaaaata attaaaaata gaagaagtac
      961 cagaagattt gaagaaacgt taggtaatcc ttgaccgtac ataattattt ttgtaagtgc
     1021 atcaccagag taggatagcg gtagaatttt accgactgtc tgagcccaac ttgccatatt
     1081 ttctagaggg attattcctg aaaagaataa ttgaggcatt ataattaagg gaataaattg
     1141 catcatttga aattctgatt ttgctagagt agacattaag attcctaatg ataaggcaac
     1201 cagagccaaa ataaaattga caatgatgac actaaaaata ctacctacaa cttgaatatc
     1261 tagtagccaa atagttgata aaacgataac tatcgtttga ataattgcta gaatgccgta
     1321 tgataacata tatccaaata caatatcact acgtttaaca ggagttgcaa ggagtcgatc
     1381 aagtgtacca gaagtacgct cttttaataa agccattcca gaaattaaaa aaacaaagaa
     1441 gaagaccata aatcccatta gtattggtat catttttgca aaaaagcctg tatttttatt
     1501 tccgtaatta tatttttctt tgatatattt ggagcgagtt tgtaaggatt gtgctagttt
     1561 cggattctta ttagctaaaa ttttaacttg cgaaatcagt tccttactgt tcattgtatt
     1621 aacagcggtt ttaaaagctt gtcttgttaa agtcgtcttt gaagaatctg tattcgcata
     1681 gaagacagta taagatttat tgtcctccga aataagagca tcaattttat ttgatttgag
     1741 tgctttttta gcagatgagt taaatttaaa tgatctcact tgaatatgct taatattatc
     1801 taaatttgaa acgaccttcg tgttaacgtt aatagttcca atcttaactt ttgtattact
     1861 attcgcagaa aacataacat tcatcaaaaa cataattaaa ataggtgcta aaaacatcat
     1921 agcaagtgtt cttttatcac gaaacagttc ttttataacc ttttcagtta ttgcaataat
     1981 tctcatgatt attctccttc agcttttaag aaaacttcct caatagtact cacattaaat
     2041 tgttttttta aatgtaatgg agtatcaaag gcaataatgt ttccacgtaa tagtagtgca
     2101 accttacttg ttaattctgc ttcatccata acgtgggttg taataaagat agaacgtcct
     2161 tcatccttaa tattaattag ctcttgccag atttttctcc tcaaggatgg atcaattcca
     2221 acggtaggtt catctaggat taaaactgtg gggtttccaa gtagggcgat ggctagagaa
     2281 agccgtcttt tcatacctcc tgagtaacct gagacaaatt tatcaagttg gttttctaga
     2341 tctactactt tagaaatatg agttatctgc tgttttaatt cagttttttg aatacctttc
     2401 atttttccaa agaataataa attttctaag ccagttaaag actcgtgtaa ggcatcagat
     2461 tgagccatat agccaatttg atttaaaata ttacgatctg gcatttgagt atcaagaaca
     2521 agagctgttc ccttatctgc tttttccatg ccaagcatag ttttaatcaa ggtagatttc
     2581 cctgctccag agggtcctat taatccaatt atttctcctt taaacacctc caaattaata
     2641 ttatttaaaa cagtttctga ggcgtatgct ttttgtagtt tttttaaatc gatgactttt
     2701 ttcatgataa ctttttgctc ctctctcagt gagtgagtgt ttactcacca atttgatatc
     2761 atatattata gtgtgtaaag acaggcttgt caataaaaga attgacagga agttaatata
     2821 ctataatagt caataaaatg gcttatttga gataactaat tttttatctt aaaaatcagt
     2881 tgcatggaga gcattcgtga aaatgggcac agaataaaac gactaagaat atccttgtcg
     2941 ttttattttt tatatagtta tacctctttt aaaattcaat ataataatgt ttattcaggt
     3001 attttttaac aatgatatga agagagaagc taagtcccag tat
//
LOCUS       DATRSG010000111         2806 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRSG010000111 DATRSG010000000
VERSION     DATRSG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.113"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            93..728
                     /locus_tag="VBD89_001961"
     CDS             93..728
                     /locus_tag="VBD89_001961"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO7136446.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            748..1611
                     /locus_tag="VBD89_001962"
     CDS             748..1611
                     /locus_tag="VBD89_001962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO7136447.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKIETIKELTKDFSRSGFE
                     GKSQVFIIKYCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            1641..1967
                     /gene="yabA"
                     /locus_tag="VBD89_001963"
     CDS             1641..1967
                     /gene="yabA"
                     /locus_tag="VBD89_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO7136448.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            1967..>2806
                     /gene="rsmI"
                     /locus_tag="VBD89_001964"
     CDS             1967..>2806
                     /gene="rsmI"
                     /locus_tag="VBD89_001964"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO7136449.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQ"
BASE COUNT          976 a          446 c          553 g          831 t
ORIGIN      
        1 ttaaaaatat cagacatgca caatcaagtt gtgggtgtct ttttttgata aattttgata
       61 gaatagtagt aattataaat aaaggatgta ccatgaaaaa aggactaatg atttcttttg
      121 agggcccaga tggtgcgggt aaaacaaccg ttttagaagc tgttttaccc ttgctcagag
      181 aaaaattatc acaagatatt ctcaccacca gagagcctgg tggcgtaact atttcagaag
      241 aaattcgtca tattatttta gatgttaaac acacgcaaat ggataaaaaa acagagctat
      301 tgctttacat ggctgctcga cgccaacatc tagtggaaaa ggtactccca gctctagaag
      361 aagggaaaat agtcctaatg gatcgtttta ttgatagttc tgttgcctat cagggatcag
      421 gaagaggatt agataagtca catataaaat ggcttaatga ctatgcgact gatagtcata
      481 aacctgattt aactttatat tttgatgttc cttctgaagt aggtttagag cgtattcaaa
      541 aaagtgttca aagggaggta aatcgtctag atctcgagca attagatatg catcaacgcg
      601 tccgtcaagg ttaccttgaa ttggcggatt cggagccaaa ccgcattgtg acgattgatg
      661 cctcccaaca gctagatgaa gtaatagctg agacattctc tattatttta gatcgtatca
      721 accaatgaat caggaagtac aaaggtcatg gatttaaagc gaacacaacc caaattgctg
      781 gaaaagttta atactatttt acagtcagat aggatgagtc atgcctatct tttttcggga
      841 aattttgcaa gtctagatat ggcactttat ttagcacaaa gtcagttctg tgaaaagcgc
      901 cagagtggtt taccatgtca agagtgtaga gcctgtcgtt taatcgctaa tggggaattt
      961 tcagatgtta aaatcataga accacagggg caacttatta agatagagac tataaaagag
     1021 ctgactaaag atttttcaag atcaggtttc gaagggaaat cacaggtctt tatcatcaaa
     1081 tattgcgaaa aaatgcatgt caatgcagcc aacagtcttt tgaaatttat tgaagaacct
     1141 caaagttcat cttatgtcat cttattgact aatgatgaaa acaatgtttt gccaacaatt
     1201 aagagtagaa cgcaaatttt tcgttttcca aaacaattgg acatgttggt tcatcaagct
     1261 gaacaagcag gattgctaaa atcacaagct agcttattag ctcaagtagc agacgatcct
     1321 aaacatttag aaatattgtt gactaacaaa aagttattag attacttgaa tttaagtcaa
     1381 cagtttgtga cgactttggc aaaagatagg caaactgctt atttagaagt atctcgcttg
     1441 acatcgcaag ttgtagataa aaatgatcag gcatttgttt ttcaatggct gacaataatg
     1501 ctggctaaag aaggccaact ttatgattta gaaaatactt atagagctca gcaaatgtgg
     1561 aagagtaatg ttagttttca aaatagttta gagtatatgg tgctttctta gaaaataata
     1621 cgcgcagaga ggaagagtga atggataaaa aagacttgtt tgatgccttt gatgattttt
     1681 cacaaaattt attagttggg ttgtctgaaa ttgagactat gaaaaaacaa atccaaaaat
     1741 tattggagga gaatactgtt ctacgcattg aaaatggtaa acttcgtgag cgtctgagcg
     1801 ttattgaagc agaaacagaa actgcagtta aaaattctaa acaaggaaga gaattactcg
     1861 aaggcattta caatgatggc tttcacattt gtaatacttt ctatggtcaa cgtcgtgaaa
     1921 atgacgaaga atgtgctttt tgtattgaat tattatatag agattgatgg aaatgcaagt
     1981 tcaaaaaagt tttaaatcaa atatacatta cggaacactc tatctagtcc caactccaat
     2041 tggtaatcta gatgatatga cttttcgtgc cattaggatt ttaagagaag ttgattttat
     2101 ttgtgcagag gatacacgaa atacgggact tttactcaag cactttgata ttactactaa
     2161 acaaattagt tttcacgaac acaatgctta cgataaaatc tctgggttaa ttgatttgtt
     2221 aaaagaaggg aaatctttag cccaagtatc tgatgcagga atgccctcta tttctgaccc
     2281 aggacatgac cttgtcaagg ctgctattga aggggatatc ccagttgtat ctataccagg
     2341 agctagcgct ggtattactg ctctcatcgc ttcaggttta gctccacaac ctcatatttt
     2401 ttatggcttc ttaccacgta agaaaggtca acaaataact ttctttgaaa caaagcaaga
     2461 ttaccctgaa acacaaatct tttatgagtc accgtttcga gtctctgata cgctaaaaca
     2521 catgaaagag atttacggag atcgccaagt tgttttagta cgcgaattga cgaaactcta
     2581 tgaagagtat caaagaggaa ccattagtca acttttagag catattgaaa aggtccctct
     2641 caaaggtgaa tgcttaatta ttgttgatgg taagagagat accgagcgag tgaaagacag
     2701 tagccaacaa gatccactag tattagtaaa agaatatatc gctaatggtg ataaaactaa
     2761 tcaagcgata aaaaaagtag caaaagaatt taatctcaat agacaa
//
LOCUS       DATRSG010000112         2764 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.114, whole genome shotgun sequence.
ACCESSION   DATRSG010000112 DATRSG010000000
VERSION     DATRSG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.114"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            282..1127
                     /locus_tag="VBD89_001965"
     CDS             282..1127
                     /locus_tag="VBD89_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO7136450.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPEYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKYAKSVMPELN
                     SYLMK"
     gene            complement(1170..2417)
                     /locus_tag="VBD89_001966"
     CDS             complement(1170..2417)
                     /locus_tag="VBD89_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7136451.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATIFGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
BASE COUNT          941 a          436 c          544 g          843 t
ORIGIN      
        1 agagatttta atgtggtttc aatttgtcta atgtaggaat aatcaccagc caaaacaact
       61 gttttttgat aagtcataaa aaatccttta tttatattag ttagatatag taaatataaa
      121 tttaaaaact attagtatta ttctatcata ggtgagtgtg attcagaaat tattattaaa
      181 ttaattatgg agtttatata gtacaacata ttctttttca gaaaaaacag ctatattttg
      241 ctatacttta atcaaatatc aatattagaa agtgattgcc tatgttgtct gttggtttag
      301 ttttagaggg tggcggaatg agaggtcttt atactgctgg agttttagat gcttttctag
      361 atgcaggaat aaaaatagat ggtatcgtat ctgtctctgc gggtgcattg tttggtgtta
      421 attttgtatc tagacaacga gagagggctt tgcgatacaa taaaaagtat ttatcccacc
      481 ctgaatatat gagtctaaga tcatggcttc gaacagggaa ttttgttaat aaagatttca
      541 cctattatga agttcctatg aaattggatg tatttgacga tgaagcattt aaaaaatcaa
      601 gtattgattt ttacgtagtt gctacagaga tgacatctgg taaacctgaa tattttaaaa
      661 ttgatagtgt ttttgaacaa atggaaattt tacgtgctag ttcagcatta ccagtagtct
      721 caaagatggt tgattggcag gggaaaaagt acttagatgg tggtttatct gatagtattc
      781 ccgttgattt tgcccgtggt ttaggatttg acaagttgat tgttgtgatg actaggccgc
      841 tcaattatca gaaaaagcct tcaagtggac gattgtataa aactctgtat aggaaatatc
      901 ctaattttgt aaagacagcc tcgaatcggt accaacagta taataatagt cttgaaaagg
      961 tcatgagcct tgaaaaaaca ggcgatctat ttgcaattag accgagtaag agcttggtta
     1021 ttggccgctt agagaagaat ccggataaac ttgatagtat ttatcagctt ggtatgaaat
     1081 atgctaaaag tgtgatgcct gagctgaata gttatctaat gaaataaaga aaacagtatt
     1141 tctcaattga ggaatactgt tttgtaatat cagtcagttg gtttgacatc taattgtgtt
     1201 aaattagtgt gatatttttg acgttttaat tgcttaatgc ttagcattga taagaaacta
     1261 acgatataga gacttgctaa gaaaagcatg accatagaga catttgaaac gtctagaatc
     1321 attccgatga taacagacga aaagccacct attgcacgac caacatttaa gataagattg
     1381 ttagctgttg aacgaatatg atgtggatag agacgtgtaa tcatggcacc atagcctgca
     1441 aacataccat taacaaagaa gccgacaact gccccgccta taatcataga aggcattgtt
     1501 gttgcaaatt gaaagagata gacacaaatt gcagatgata gaaggaaaca cccgtatact
     1561 aaacgcggtc caaatttatc taacaattga ccaaatacta gcatgccaag acacattcca
     1621 aaaatggttg caatcatcca taatgatgaa ttcttgacag aaacgtttaa gttggtttgg
     1681 ataatagttg gtaaccagtt catcatgcca aagtatccgg caatctgaac agttgtcata
     1741 accattagtg ctaggctctg tgtggctaat acaggagtat caaataaagc ttttatactg
     1801 atatctagag gttcatcatc tgcgtctgta ttatattgat ctaagatatc tttatcatca
     1861 atagcaaatt gcatccatag gacgagaact ataggaagaa ggccaaagag aaaaagtccc
     1921 cgccaaccga gtgctggtgc tagccatcct gctaagagtg ctgagctaat agatccgact
     1981 tgaccagcga taccatttag tgaagaaatc cggcccattt tattagctgg gacaattcct
     2041 gccataatgg caatagcgat accatattcg ccaccaacgc caattcctgc aatgaagcgc
     2101 atgatataga gatatgataa attagtagtg aagaaaacta aaccagttgc gattgaaaag
     2161 attagaatcg tccatttgaa aactttgaat ttattgtagc gatcagctaa taaaccaaag
     2221 attagtcctc ccacgagcat tcccaagttg gtaattgtcc caatccaacc agcctgtgcc
     2281 gcagataaat gaagatcagt aatgatagtt gacatagaaa atgcaaggaa catgatattt
     2341 aaatcatcgg ttccggatgc gacaatggca gcgattaggg cgcgccaatt attcttattg
     2401 atatttaatg gtgacataag tctcctcctt taatgatggc tcacagacca ccgttattaa
     2461 atactgtcaa tagaaaaaat tctaaagcag agatgtcata gctgcttcaa ctcattataa
     2521 tgtaaaagaa aaaactcaag ctgctaccct tgagttctat aaacgctcgc aaaaatagta
     2581 ggatttctgt atagaatcct attttggtcg cttatatgtc tcacaggaca cggttaaagc
     2641 attatgtcat ttggaaatag tatagagtaa gtttagttta ttgtcaagga taagtgggga
     2701 gagaacgcta aattctcaaa gtaagttcca cctctagtag aaatggaatt tagtctgata
     2761 aaaa
//
LOCUS       DATRSG010000113         2718 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRSG010000113 DATRSG010000000
VERSION     DATRSG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.115"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(77..1459)
                     /locus_tag="VBD89_001967"
     CDS             complement(77..1459)
                     /locus_tag="VBD89_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7136452.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            complement(1467..2681)
                     /locus_tag="VBD89_001968"
     CDS             complement(1467..2681)
                     /locus_tag="VBD89_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7136453.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
BASE COUNT          927 a          574 c          420 g          797 t
ORIGIN      
        1 tattttaaat ttctttccca attcctttta cattattaca acaatacttg agattttgaa
       61 tctcaagtat tgttatttat ttatcctcaa ttttcttagg gataacaaaa agaattgata
      121 agatcgatag actacaaaaa actaggttta aaattaatcc acacaaagct gctttgtgaa
      181 aatctgcatt accagaaaaa gcatgataaa tcgcaattga agttgctaca ccaattgcac
      241 cacctagtga acttgccatt ttgtaaattc cagatgcaga acctactttt tcattaggaa
      301 tactagaaat agctgtatct gttgatggtg tcgcatagat ccccaagcct gttccaaata
      361 aagcataacc aacaaagact aacacaagat acaaaggtcc ttgaatattg accaaagtca
      421 ttaaaaatat cccaacaaat gtactcatag ctcccaaaag cattggctta cgggcaccaa
      481 aacgttgtaa aattttctca ccaactcgga tagcaattag tacacaaacc aaatagccta
      541 aagacatttc acctgcaacc tttggtgtta gctggcgacc ttgttgcatg taagtattaa
      601 taacaatcag agttcctgca actgcattca gtaagaaatt tgatattgtc gcacctaagt
      661 aaaatcgatt ttcaaacaga tgaaagtcaa taaaactatt agatttacgt gtttcaacat
      721 aataaaacag aacaaatcca ataagcatca ctgtaaaaag actcaatgga ataacgttca
      781 tcaatccatg ttcctgagcc atagatatcc caatatttaa cgataacatt gaaataataa
      841 aaataattaa accaaggtaa tcaaagtgag ttttttgtcc aacattttta ctttctgggg
      901 ttcctaaaat taatagaaaa ctaactactg atgcgatgat tgaaaagata aagacataac
      961 gccaacctag agtacttgcc accgctccac caaaataaga acatagacct gagccacccc
     1021 acgatccgat tgaccagaaa ctaactgcac gttgtctatc tttaccatca tagtacgttt
     1081 taaccaaggc cattgttgat ggcataataa aagctgctgc caatccttga aaaatacgtc
     1141 ccataataaa tagcactgct ccattagcta aaacaattag tagagaacca ataatattta
     1201 agcaaagacc aataaaagta aatttaactc gaccaagttt atctgctaaa ccaccagtta
     1261 caacaataaa taaacctgaa aacaatgccg tacttgagac accaatatcc attgcaccag
     1321 atgaaatacc taatgatgac tgaacatctg gtcccatgtt taaaatagac tgtgcaaata
     1381 accaaaatga tagtacagcg agtacaattc ccaccaataa tttgttattt cctttgaaat
     1441 tttcctttgt cgttaacata tgttatctat tctccttcta caagcatagc aagcgctgct
     1501 gttgccattg atttagcaga taccatcaat gatgattctg caatttggaa ttttggatga
     1561 tgatgtgggt aatatggttg cccctcttca tgtgctccaa catagaagaa gcaagctggg
     1621 attgtttgag cataataggc aaaatcttca gacggcgttt gagggtcgca atcaatgaca
     1681 tctaaaattt cttttatagc aacagatttt aaactatttg caactttctg tgtcacttca
     1741 ctgttattaa ctaaaacagg ataatcattc tgatagtcta actgataact aacaccataa
     1801 gtttgtgcta ttccatctag aatacgctta aattcctcct caacaacacc tcgtgtctct
     1861 tcagacatca cgcgcacatc accttctaag gttacactat ctttgatcac attagcagaa
     1921 cctttaccat caaaagatcc gatagtaact actgctgtgt caaatggatt aactcgacga
     1981 ctgacaatcg tttgcgctgc cataacaaaa ctagaagctg caacaattgt atcatttgca
     2041 cgatgtggca tggaaccatg tccaccctta ccttgcaaaa taactttaaa agtggcacgc
     2101 cctgtttgaa tgggacctgc gtgatattgt accgttccct cttccatcgt tgacataaca
     2161 tggataccaa ggacagcatc aatgccatct aaacatcctg cttcaatcat tgcttttgct
     2221 ccgcctggtg gcacctcttc agccggttga tgtaagatac ggatatgacc tgaaaattct
     2281 gattttaatt caatcaagct ctcagccaaa atcaataaat aagctgtatg ggcatcatgt
     2341 ccacaagcat gcatgacgcc cgctgttttt gaagcaaaag aaagccctgt ctcttcctga
     2401 attggaagag catcaaagtc ggctctcaaa gcaatgtgtt tgatgggctt atctgtcgct
     2461 ttatcaccat agatatcaac tacgacgcca ttcataccac caaattgtgt ctgaacatga
     2521 cagtccttac ctttataaaa atctgaaata taagctgctg tttttgtctc tttaaatgat
     2581 aattcgggat tttcatgtaa atagcgacga atagcaacca tttgatcaca tttttggtca
     2641 agtttttcaa ataattgatg tcgcaaagat gcattggcca tttgctttcc tccttaaaat
     2701 atgaacagtt aaaataag
//
LOCUS       DATRSG010000114         2676 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRSG010000114 DATRSG010000000
VERSION     DATRSG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.116"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..952
                     /locus_tag="VBD89_001969"
     CDS             <1..952
                     /locus_tag="VBD89_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7136454.1"
                     /translation="SKMTFRNKYDHNYIAVCILDSSEKDCYDLKHNSLRIINKDALTS
                     ELTCLTVDQAFINIPIELFGKYQIQDIINDIEAMGVIVNVNVEALSFDNIGEKRIQTF
                     EGYSVITYSMKFYKYSHLIAKRFLDITGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQ
                     NRVGRNGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFKLEDDPRITKIGKFIRKT
                     SIDELPQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNI
                     TDFDEIVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            976..1425
                     /locus_tag="VBD89_001970"
     CDS             976..1425
                     /locus_tag="VBD89_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO7136455.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1425..1898
                     /locus_tag="VBD89_001971"
     CDS             1425..1898
                     /locus_tag="VBD89_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO7136456.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLLVVVPRRKQFGEHIND
                     HQIQFLKKIAHLYPLAWIEDVDGLAEALKRNIATEKYQGNNDMFCHKLEKIIGEI"
     gene            1895..>2676
                     /locus_tag="VBD89_001972"
     CDS             1895..>2676
                     /locus_tag="VBD89_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein CapK"
                     /protein_id="HEO7136457.1"
                     /translation="MRKYLDLDYSLFYALWVLILVPNQWYQFLIITIIVLLLLWKSEF
                     RISISNSSILFLLWLFIYLFAILIRGTQEDITFQRFIAELLKLISTGYALFFYNYYRK
                     ADFNSSVVRNVVKVNYFVLFLITVLYLFFPMLKPTLFGRELFSIEWFPHMRIRLAAYF
                     EYATLIGQFILFSYPILFLKPQKHMENILISLLLTICSYFSGARILLVCMLVLLASLL
                     LDYILFKTNLKLTKKNTFILGMTFLFITACFSYNIWSIIEKI"
BASE COUNT          896 a          354 c          490 g          936 t
ORIGIN      
        1 atcaaaaatg acctttagga ataaatacga ccataattat atcgctgtct gtatcttgga
       61 ctcctctgaa aaggattgtt atgatttgaa acataactcg ttaaggataa taaacaaaga
      121 tgctcttact tcagagttaa cctgcttaac tgttgatcaa gcttttatta acatacccat
      181 tgaattattt ggtaaatacc aaatacaaga tattattaat gacattgaag caatgggagt
      241 gattgtcaat gttaatgtag aggcacttag ctttgataat ataggagaaa agcgaatcca
      301 aacttttgaa ggatatagtg ttattacata ttctatgaaa ttctataaat atagtcacct
      361 tatagcaaaa cgatttttgg atatcacggg tgctattata ggtttgctca tatgtggcat
      421 tgtggcaatt tttctagttc cgcaaatcag aaaagatggt ggaccggcta tcttttctca
      481 aaatagagta ggtcgtaatg gtaggatttt tagattctat aaattcagat caatgcgagt
      541 agatgcagaa caaattaaga aagatttatt agttcacaat caaatgacag ggctaatgtt
      601 taagttagaa gatgatccta gaattactaa aataggaaaa tttattcgaa aaacaagcat
      661 agatgagttg cctcaattct ataatgtttt aaaaggtgat atgagtttag taggaacacg
      721 ccctcccaca gttgatgaat atgaaaagta taattcaacg cagaagcgac gccttagttt
      781 taagccagga atcactggtt tgtggcaaat atctggtaga aataatatta ctgattttga
      841 tgaaatcgta aagttagatg ttcaatatat caatgaatgg tctatttggt cagatattaa
      901 gattattctc ctaacactaa aggtagtttt actcgggaca ggagctaagt aaaggtaagg
      961 tttgaaagga atataatgaa aatttgtctg gttggttcaa gtggtggtca tctagcacac
     1021 ttgaaccttt tgaaacccat ttgggaaaaa gaagataggt tttgggtaac ctttgataaa
     1081 gaagatgcta ggagtattct aagagaagag attgtatatc attgcttctt tccaacaaac
     1141 cgtaatgtca aaaacttggt aaaaaatact attctagctt ttaaggtcct tagaaaagaa
     1201 agaccagatg ttatcatatc atctggtgcc gctgtagcag taccattctt ttatattggt
     1261 aagttatttg gttgtaagac cgtttatata gaggttttcg acaggataga taaaccaact
     1321 ttgacaggaa aattagtgta tcctgtaaca gataaattta ttgttcagtg ggaagaaatg
     1381 aaaaaagttt atcctaaggc aattaattta ggaggaattt tttaatgatt tttgtcacag
     1441 tggggacaca tgaacagcag ttcaaccgtc ttattaaaga agttgataga ttaaaaggga
     1501 caggtgctat tgatcaagaa gtgttcattc aaacgggtta ctcagacttc gaacctcaga
     1561 attgtcagtg gtcaaaattt ctctcatatg atgatatgaa ctcttacatg aaagaagctg
     1621 agattgttat cacacatggc ggcccagcga cgtttatgtc agttatttct ttagggaaat
     1681 tactagttgt tgttcctagg agaaagcagt ttggtgaaca tatcaatgat catcaaatac
     1741 aatttttaaa aaaaattgcc cacctgtatc ccttggcttg gattgaagat gtagatggac
     1801 ttgcggaagc gttgaaaagg aatatagcta cagaaaaata tcagggaaat aatgatatgt
     1861 tttgtcataa attagaaaaa attataggtg aaatatgagg aaatatctag atttagatta
     1921 ttctttattt tatgctcttt gggtacttat tttagtacca aaccaatggt atcagttttt
     1981 aattattacc attatagttc tattattact ttggaagagt gagtttagaa tatctataag
     2041 caattcttca atactatttc tgctttggtt atttatttat ttatttgcaa tactcattag
     2101 aggtactcaa gaggatataa cgtttcagcg atttattgct gagctattaa aactaattag
     2161 tacaggatat gctttatttt tttataatta ttatagaaaa gctgatttta atagttcagt
     2221 tgtaaggaat gtggtaaagg ttaactattt tgtgttgttt cttataacag ttttatattt
     2281 attttttcct atgctgaagc caactttatt tggaagagaa ttgttttcaa tagagtggtt
     2341 tccacatatg agaataagac ttgcggcata ttttgaatat gctacactaa ttggtcagtt
     2401 tattttattt tcttatccca tacttttttt gaaaccccaa aaacatatgg aaaatatttt
     2461 aatatcctta ctgttgacta tatgttcata cttttctggc gctagaatac tattggtctg
     2521 tatgttggtt ttattagctt cgcttctttt agattatatc ctttttaaaa ctaatttgaa
     2581 attgaccaag aaaaacactt ttatacttgg tatgactttc ttatttatca ccgcttgttt
     2641 ttcttataac atatggtcaa taattgaaaa aataat
//
LOCUS       DATRSG010000115         2353 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRSG010000115 DATRSG010000000
VERSION     DATRSG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.117"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..97)
                     /locus_tag="VBD89_001973"
     CDS             complement(<1..97)
                     /locus_tag="VBD89_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7136458.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNL"
     gene            300..2339
                     /locus_tag="VBD89_001974"
     CDS             300..2339
                     /locus_tag="VBD89_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7136459.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSSYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
BASE COUNT          814 a          413 c          438 g          688 t
ORIGIN      
        1 gtagattaag cccgatggct ttaaaggttt ctcctctatc caaaccagat tggatgtcat
       61 tacggtctga gagagttagg tgtttgtgtt ttgtcatgat ggataaactc atttctaacc
      121 caaacgtctc agacttaatt ccaccataaa aatccttttc acactaactt ccggttttgg
      181 agggcagacg gaacttactt tgagaaatta cttaatataa aataacagtt aatattttag
      241 caatttgata acacttagat tataatgtaa acgtatactt ttttagattg gaagatgaaa
      301 tgaaacgtaa atactttgtt cttaatacgg tgacggtttt aacgttagct actgcaatga
      361 atactagcag tagctatgct aatagtactg agacaagtgc ttcagtagct cctactacaa
      421 atactatcgt tcaaactaat gacagtaatc ctaccgcaaa atttgcatca gaatcaggac
      481 aatctgtaat aggtcaagta aaaccagcta attctgcggc gcttacaaca gttgacacgc
      541 ctcatatttc agctccagat gctttaaaaa caactcaatc aagtcctgtc gttgagagtc
      601 cttctactaa gttaactgaa gagacataca aacaaaaaga tggtcaagat ttagccaaca
      661 tggtgagaag tggtcaagtt actagtgagg aactcgtcaa tatggcatac gatattatcg
      721 ctaaagaaaa cccatcttta aatgcagtca ttactactag acgccaagaa gccattgaag
      781 aggctagaaa acttaaagat actaatcagc cgtttttagg tgttcccttg ttagtcaagg
      841 ggttagggca cagtattaaa ggtggtgaaa ccaataatgg cttgatctat gcagatggaa
      901 aaattagcac atttgacagt agctatgtca aaaaatataa agatttagga tttattattt
      961 taggacaaac gaattttcca gagtatgggt ggcgtaatat aacagactct aaattatacg
     1021 gtccaacgca taatccttgg aatcttgctc ataacgctgg tggctcttct ggtggaagtg
     1081 cagcagctat tgctagcgga atgacgccaa ttgctagcgg cagtgatgct ggtggttcta
     1141 tccgtattcc atcttcttgg acgggcttag taggtttaaa accaacaaga ggattggtga
     1201 gtaatgaaaa gccagattcg tatagtacag cagttcattt tccattaact aagtcatcta
     1261 gagacgcaga aacattgtta acttacctaa agaaaagcga tcaaacgcta gtatcagtta
     1321 atgatttaaa atctttacca attgcttata ctttgaaatc accaatggga acagaagtta
     1381 gtcaagatgc taaaaatgct attatggaca acgtcacatt cttaagaaaa caaggattca
     1441 aagtgacaga gatagattta ccaattgatg gtagagcatt aatgcgtgat tattcaacct
     1501 tggctattgg catgggagga gctttttcaa caattgaaaa agacttaaaa aaacatggtt
     1561 ttactaaaga agacgttgat cccattactt gggcagttca tgttatttat caaaattcag
     1621 ataaggctga acttaagaaa tctattgtgg aagcccaaaa acatatggat gattatcgta
     1681 aggcaatgga gaagcttcac aagcaatttc ctattttctt atcgccaacg accgcaagtt
     1741 tagcccctct aaatacagat ccatatgtaa cagagaaaga taaaagagcg atttataata
     1801 tggaaaactt gagccaagaa gaaagaattg ctctctttaa tcgccagtgg gagcctatgt
     1861 tgcgtagaac accttttaca ccaattgcta atatgacagg actcccagct atcagtatcc
     1921 cgacttactt atctgagtct ggtttaccca tagggacgat gttaatggca ggtgcaaact
     1981 atgatatggt attaattaaa tttgcaactt tctttgaaaa acatcatggt tttaatgtta
     2041 aatggcaaag aataatagat aaagaagtga aaccatctgc tgacctaata cagcctacta
     2101 actccctctt taaagctcat tcatcattag taaatttaga agaaaattca caagttactc
     2161 aagtatctat ctctaaaaaa tggatgaaat cgtctgttaa aaataaacca tccgtaatgg
     2221 catatcaaaa agcacttcct aaaacaggtg atacagaatc aagtctatct ccagttttag
     2281 tagtaaccct tttattagct tgttttagct ttgtaacaaa aaagaatcag aaaagttgat
     2341 tgtaatgtaa aat
//
LOCUS       DATRSG010000116         2195 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRSG010000116 DATRSG010000000
VERSION     DATRSG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.118"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            195..1535
                     /locus_tag="VBD89_001975"
     CDS             195..1535
                     /locus_tag="VBD89_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO7136460.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
     gene            complement(1637..>2195)
                     /locus_tag="VBD89_001976"
     CDS             complement(1637..>2195)
                     /locus_tag="VBD89_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136461.1"
                     /translation="NFASIFPYFLGLLLVNNIWTVFFTSNLIGLSTIIIFAYCILLVI
                     IIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVYLVKIDFNYPLPKVYIAIIALIF
                     ITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGNFQHLYFSFQILSLIAAIILGVS
                     FFIIGYYNVKERYKQKRTSPYSRVL"
BASE COUNT          700 a          395 c          338 g          762 t
ORIGIN      
        1 ttaggtaaat ctacggaact aaaaataatc gatagaaatc tgttattttg ttatttcaaa
       61 tgtaactgtt aagaaaatag ttctctaata ctaaacagca cctcaatcga ttattaagta
      121 gcattaattt ttaattgttt ttctattttc tatttgttat aatggcttga gattactaga
      181 aagtatagta ttacatgaat caaaatcatt ccgtcaaaag atccttatcc gaagttaatc
      241 agagcgttga ggttcctcac aatagctctt tttggaacac tttaagagct tttcttggac
      301 ctggagcttt ggttgcagtt ggttatatgg atccaggtaa ctggattact agcgttattg
      361 gtggagctac ctatcgttac ttactattgt ttgttgtttt agtatcttct ttgatggcga
      421 tgcaattaca acaaatggct ggcaagcttg gtattgttac acgacaggac ttagcacaag
      481 caacagcttc tcgactccca aaaccgcttc gttacctgct atttattatc attgagttag
      541 cccttatcgc tactgattta gcagaagtca ttggctctgc cattgctctt cacttattat
      601 ttggctggcc cctgttactg tctatcatga ttactatttt ggatgtattc ttacttttac
      661 tcttaatgaa gttaggcgtc caaaaaattg aagcctttgt ttctgttctc atattgacaa
      721 ttttgataat ctttacatat ttagttgttt tatcacaacc tgatttagat gctatgttca
      781 aaggattttt accacaccat gagcttttta acatatcaca tgaagggaaa aatagtcctt
      841 taaccctcgc tcttggcatt atcggcgcaa cagtcatgcc acataatctt tatttacatt
      901 cttctttatc tcagactaga cgcgtggatt atcataataa atcttcaata aaaaaagctg
      961 tacgtttcat gaccttagat tctaacattc agctatcact tgcatttgtc gtgaattcgc
     1021 tactattagt tttaggagct agtctatttt atgggcatgc taatgatatc agtgcctttt
     1081 ctcaaatgta tcttgcctta tctgataaaa ctattactgg tgcagtagcc agttctttcc
     1141 tttctaccct ttttgcagtt gccctactag caagtggtca aaattcaaca attacaggta
     1201 ctttaacagg acaaatcgtt atggaaggtt tccttcactt caaattacct caatggttaa
     1261 taaggctttg tactagatta ttaactcttc ttccaatttt tgctatcgcc ctactagttg
     1321 gaggcgaaga aaatacgtta gatcaactaa tcgtctattc acaagttttt ctttccttag
     1381 ctttaccatt ttcaatcttt ccattgattt atttcacatc gcaaaaatca attatgggag
     1441 agcatgctaa tactaaatgg aatacctatt tagcatacct tatcgctatc attttaacat
     1501 tactcaattt gaaattaatc atggatttac tttaatacaa tacaaaaaag ccccactaat
     1561 gaactgaacc tctatcatga gacatacatc taatgataga ggaaagtaca taatgatgga
     1621 gctttttgta tttcttttaa agaacccgtg agtaaggact agtacgtttt tgcttgtacc
     1681 tctccttgac attataataa ccgatgatga aaaacgatac gccaaggata attgctgcaa
     1741 tcaaagataa aatttggaaa ctaaaatata ggtgttgaaa atttccttct aaatgtgctt
     1801 taaaaaccat caaccaagcc caaaaaacgg ataagattaa gtaagcattt tgtaataccc
     1861 tagccaggta aagacttaaa acagttataa aaatcaaggc aatgatagct atatatacct
     1921 ttggtaatgg gtaattaaaa tcaattttaa ctaagtagac cgctaaattg accaatgacg
     1981 caactagcaa ccatcctgca tgaattccaa atgttattct aagcagtaat tttgatttat
     2041 ttttacttag tatcttgata atgataacca gaagaataca gtaagcaaaa attattattg
     2101 ttgataggcc aatcaaattg ctcgtaaaaa atacagtcca tatattatta actaatagaa
     2161 gacccaaaaa gtatggaaaa atgctagcaa agtta
//
LOCUS       DATRSG010000117         2085 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRSG010000117 DATRSG010000000
VERSION     DATRSG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.119"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(254..625)
                     /locus_tag="VBD89_001977"
     CDS             complement(254..625)
                     /locus_tag="VBD89_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO7136462.1"
                     /translation="MKKKLLGLMILAISTVFLVACSTNSLNVKYYKVYDGEKMLVLEI
                     NDESGIYHNEGNFVVTNIDTKSKSFVLKGGSAKYTVLYDVKSDGKLNVDLGNFYYNAS
                     GKETYYKEGSKALEKALRDKE"
     gene            complement(778..966)
                     /locus_tag="VBD89_001978"
     CDS             complement(778..966)
                     /locus_tag="VBD89_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO7136463.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            complement(963..1256)
                     /locus_tag="VBD89_001979"
     CDS             complement(963..1256)
                     /locus_tag="VBD89_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO7136464.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            1313..1462
                     /locus_tag="VBD89_001980"
                     /pseudo
     CDS             1313..1462
                     /locus_tag="VBD89_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1968..>2085)
                     /locus_tag="VBD89_001981"
     CDS             complement(1968..>2085)
                     /locus_tag="VBD89_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7136465.1"
                     /translation="KGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED"
BASE COUNT          749 a          378 c          221 g          737 t
ORIGIN      
        1 tcatcaatta aataaaagct atctcatttc ctcgcttgat ttcattgaat taacagataa
       61 cactttccaa gaaaaactaa gacagagtaa ccccatcttc tttttggttc ttactttgat
      121 ttcggtaaaa atacttttta ttgctgggat acaaattatt taactaaaaa ttaggtgcta
      181 agtaaaatta agactttgca ttttgaagtt cattttttaa ataaaaaaat aagttaggaa
      241 aatagtccta actttattct ttatctctta atgctttttc taaagccttg ctaccttctt
      301 tataatatgt ctccttacca gaagcattat aataaaagtt ccctaaatca acattcaatt
      361 taccatcact tttaacatca tataaaacag tatacttagc agacccacct ttaagtacaa
      421 aacttttact tttagtatca atattagtta ctacaaagtt tccttcatta tgataaattc
      481 ctgattcatc attaatttct aaaactaaca ttttttcacc atcatatact ttataatact
      541 ttacatttaa cgaattagtc gaacaagcaa ctaaaaatac tgtagatata gctaaaatca
      601 tcaatcctaa taatttcttt ttcataacta aacttccttt gctatgagaa aatcttttaa
      661 cctctcaaca tctctatcgc ttaacttacc tataatttta atattgaacc ctttattctc
      721 aacatcataa tattgaatag tatctatcca agaacgccta tttaaatttg cttttgccta
      781 atcaataact tctaaatact gacttcttat attgtcagac ttattttcat atttagtagt
      841 aaatcttaaa gtttttatta cttcatcatt aaatttgact actacagcag gacgaacctt
      901 tgaacctgta ccatcagaat attcaatacg agaaactaat acagaatata cctcaatatc
      961 actcatttaa aatattcctc atattctgaa aaatcttcat ctccaaaaaa cgcatttaca
     1021 ctttctggat cactagcctt aactcgtatt ccctttaatt ttgtttctac caaattttga
     1081 aattcttcat cagaaacaga tgatttattt tttatttcaa aaggaatact ttgagtttta
     1141 acagattgaa tcaaaaacat ttttactgct gtgctcatat ccaaccctaa ggattgaaaa
     1201 agttctgtag catcatcttt taattgatca tcaactctta ctgctactgt tgacataata
     1261 tcacctcaaa tcatttttgt ataactattg tatcactaaa tgcaatacaa cgcaaacaaa
     1321 aaaggaaatc gtctttccca atgaagaggc tctagaatac tatctagtca cactatttaa
     1381 tgattacaac ttcaagcaag aacaacgaat ccacaaaggt tttggccaat gtacagacac
     1441 acttgaaagc ctatttgatt aacaatccca gctttgccag tgtgtttaca caacattatt
     1501 gacacactcc ttttattttt ataaattacc cttaaataca tttaattact tgaaaagaca
     1561 aacagtcatt ttagaaaatt ttgcatctaa ccttacattt gattacacct aaaaatttta
     1621 aatcatcaaa agagagccta ggattaacat cttaggctct aatgatacac ttcatttaca
     1681 aatttcaact aaataggatc atttcttcag ttttatagta agcgtccaat aacaaggtat
     1741 attggatgct tacggaatag ggaagaaaat ggaagatacc tcgttattgg acgcttacac
     1801 aaaatcaaaa tcctatcgaa attaataatc tcgataggat ttttcaagaa ttaaactcga
     1861 aaaattgaaa cactttaatt aagtttgttg aagagagata cataacttta ttaatgtgtt
     1921 cacaccaata aatggtgaaa agtttagtga cattttaggt caaaagatta atcctctttt
     1981 ttcttagaaa cagataataa accaactgat gacataattg tcaaagctac aacattaaag
     2041 aatggagttg cattctcacc tgttgctggt agtttatttc cttta
//
LOCUS       DATRSG010000118         2056 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRSG010000118 DATRSG010000000
VERSION     DATRSG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.120"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            2..72
                     /locus_tag="VBD89_001982"
     tRNA            2..72
                     /locus_tag="VBD89_001982"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Gly,seq:tcc)
     gene            109..182
                     /locus_tag="VBD89_001983"
     tRNA            109..182
                     /locus_tag="VBD89_001983"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:143..145,aa:Ile,seq:gat)
     gene            193..264
                     /locus_tag="VBD89_001984"
     tRNA            193..264
                     /locus_tag="VBD89_001984"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:226..228,aa:Glu,seq:ttc)
     gene            278..367
                     /locus_tag="VBD89_001985"
     tRNA            278..367
                     /locus_tag="VBD89_001985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:314..316,aa:Ser,seq:tga)
     gene            378..451
                     /locus_tag="VBD89_001986"
     tRNA            378..451
                     /locus_tag="VBD89_001986"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:412..414,aa:Met,seq:cat)
     gene            454..526
                     /locus_tag="VBD89_001987"
     tRNA            454..526
                     /locus_tag="VBD89_001987"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:487..489,aa:Phe,seq:gaa)
     gene            538..618
                     /locus_tag="VBD89_001988"
     tRNA            538..618
                     /locus_tag="VBD89_001988"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:572..574,aa:Tyr,seq:gta)
     gene            625..695
                     /locus_tag="VBD89_001989"
     tRNA            625..695
                     /locus_tag="VBD89_001989"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Trp,seq:cca)
     gene            710..782
                     /locus_tag="VBD89_001990"
     tRNA            710..782
                     /locus_tag="VBD89_001990"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:743..745,aa:His,seq:gtg)
     gene            789..860
                     /locus_tag="VBD89_001991"
     tRNA            789..860
                     /locus_tag="VBD89_001991"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:821..823,aa:Gln,seq:ttg)
     gene            871..954
                     /locus_tag="VBD89_001992"
     tRNA            871..954
                     /locus_tag="VBD89_001992"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:905..907,aa:Leu,seq:caa)
     gene            1366..1630
                     /locus_tag="VBD89_001993"
                     /pseudo
     CDS             1366..1630
                     /locus_tag="VBD89_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1870..>2056
                     /locus_tag="VBD89_001994"
     CDS             1870..>2056
                     /locus_tag="VBD89_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136466.1"
                     /translation="MAESANTVRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKGN
                     QITQGGSNLPNDGSVYLQ"
BASE COUNT          645 a          347 c          467 g          597 t
ORIGIN      
        1 agcgggtgta gtttagtggt aaaactacag ccttccaagc tgttgtcgcg agttcgattc
       61 tcgtcacccg ctttacttta taagaaagta aaaaccaagc atttgcttgg gcgcgtagct
      121 caggtggtta gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc cactcgtgcc
      181 catcaagaat atggtccgtt ggtcaagggg ttaagacacc gccttttcac ggcggtaaca
      241 cgggttcgaa tcccgtacgg actatttttt gttatatgga ggattaccca agtccggctg
      301 aagggaacgg tcttgaaaac cgtcaggcgt gtaaaagcgt gcgtgggttc gaatcccaca
      361 tcctccttta aagatatcgc gggatggagc agctaggtag ctcgtcgggc tcataacccg
      421 aaggtcgtag gttcaaatcc tgctcccgca ataggctcgg tagctcagtt ggtagagcaa
      481 tggattgaag ctccatgtgt cggcggttcg attccgtctc gcgccattta taccttagga
      541 gagatagcga agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt
      601 cgaatccctc tctctccata gtaaacgggc atagtttaaa ggtagaacta aggtctccaa
      661 aaccttcagt gtgggttcga ttcctactgc ccgtgttttt tctaatatgg cgggtgtggt
      721 gaagtggtta acacatcaga ttgtggctct gacattcgtg ggttcgattc ccatcactcg
      781 ccttttcatg gggtatagcc aagcggtaag gcaagggact ttgactccct catgcgttgg
      841 ttcgaatcca gctaccccag ttttaagata gccggcgtgg cggaattggc agacgcgctg
      901 gactcaaaat ccagtgtcct cacggacgtg ccggttcgac cccggccgcc ggtatacttt
      961 aaaagacaag gtttctaaac cttgtctttt tttgttttgt actggtaatt ttaagaaaaa
     1021 gttactgaaa attacttata tttgtttttc attattgcaa ttgttattga agttttaaaa
     1081 caagcagaaa taataaggaa atttttcagg agttagtgga gactcaacat ttaaatgaaa
     1141 ttcaaacagg actcctaaat gtcataggtt ctgaagtaat aaaagtcttt gtgaatgata
     1201 gataggtagt ataaaataat aagatttttc agtgtatatg ttacgactaa aaaattaaac
     1261 tcgtgaaaat ttgttatact aatattagtt ttaaaatact ggtaaataat aattattttt
     1321 attagatact tgacatggga agtttatttt ctaagaaaag agagcatgat gaaaaaatat
     1381 gtaaaatggt taattcccat atctattatt gggatgttac tagcaggttg tcaaatgaat
     1441 agtgaatcta aaagtcagtc aaacgaagta aaaaatagca aacagtcagc agtaaagaaa
     1501 ggtaaaaaag taataaaaaa agaacaagta gcttatctca aaaagtatga aaaagaatta
     1561 actgaatatg tcaaaaccca aaatccagat attagcgaaa ttaagtatga ttggaatagt
     1621 gtgcgtatag tggaagctag taatggaacc cctcaaggca gagagcagct attattaatt
     1681 tggggttatg caaatggcag tgaattaacc aattttactc taaatttcac attgggtgat
     1741 aatcaaatgc ctaatattga ctcagttggc tcagataatt tatcaactat tgaagattaa
     1801 taataacagt aagaaaggta tataatggga tgttggaatc ttaaggaatt tgaagatata
     1861 ttttgaagta tggcagaaag tgctaataca gttcgatcga attcttttgt tttagaagaa
     1921 ttaactgaat ttcaaagaga aaagttaaaa aatggggagt cagttaagtt taatttttct
     1981 aaagatgtta agaaaggaaa tcaaatcacc caaggtggct caaatctccc aaatgatggg
     2041 agtgtctatc ttcagc
//
LOCUS       DATRSG010000119         2041 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRSG010000119 DATRSG010000000
VERSION     DATRSG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.121"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..292
                     /locus_tag="VBD89_001995"
     CDS             <1..292
                     /locus_tag="VBD89_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136467.1"
                     /translation="NFIDDFLDIKWEKVSNIHLRFWTTIIAYLVIFILSISTVILNLV
                     LLFQGFLTQNPIIYLLFFITLVCAFYFAYKFITYTPTIVKNALQYIKKLKNV"
     gene            complement(385..543)
                     /locus_tag="VBD89_001996"
     CDS             complement(385..543)
                     /locus_tag="VBD89_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136468.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
     gene            complement(569..1882)
                     /gene="obgE"
                     /locus_tag="VBD89_001997"
     CDS             complement(569..1882)
                     /gene="obgE"
                     /locus_tag="VBD89_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO7136469.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            complement(1943..>2041)
                     /locus_tag="VBD89_001998"
     CDS             complement(1943..>2041)
                     /locus_tag="VBD89_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO7136470.1"
                     /translation="KTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
BASE COUNT          627 a          465 c          287 g          662 t
ORIGIN      
        1 caactttata gatgactttt tagatattaa atgggaaaag gtgagtaaca tccatttaag
       61 gttttggaca actattattg cttatctagt tatttttatc ttaagtatct ctaccgtaat
      121 tcttaacctt gtacttcttt ttcaaggatt cttaacgcaa aaccctatta tttacttact
      181 attttttata actttagttt gtgcttttta ttttgcttat aaatttatta cttatactcc
      241 aaccattgta aaaaatgctc ttcagtatat caaaaagctc aaaaacgttt aaaataataa
      301 agtaatctgt accttaacac tctatcaatt atgttttaga gtcttttttc atacccaaaa
      361 taactaaaaa ggatagttac ttaactatcc tttttctttt gctaattttt cgcgttctaa
      421 acgaagttta cgattaggat ttaatgtgtt aaagagttct tctaattttt cagcattcca
      481 gacatcggcc gctgaaacaa agttcccatc tttatcacga aatgatatcg gtttatctcc
      541 cataaaaaac ctccaatcaa gtactgcatt aatctacaaa ttcaaattcg aagttaccta
      601 tacgaacaat atctccatcc ttagctccgc gctctcgaag tgcttcatca acgcccatac
      661 cacgtaactg acgagcaaat ttcattattg attcatcgcg ttccatattt gtcataacaa
      721 agagtttttc tagcttatca ccatacaata cccacgttgc atcatcatca cgtgtaatct
      781 caaatggacg ttcatcttcg ttaaatccat agtatgcttc atcttcttgc atgtcagttt
      841 cgtcataaag aagaaactct tctgtattgg ctaataattt agccgtcgca tccataaggt
      901 tctctagtcc ttgatgtgct aaacttgaaa ttggaaaaat cataggcatg tcatcaaact
      961 catcatagtt tgcagctaat ttttctttga atgctgccaa attctcttct gaatcaggca
     1021 tatccatttt attggcaaca ataatttgcg gacgttccat caatcgaaga ttgtaagttt
     1081 ccaactcatt attaattgaa atatagtcat cgtaagggtc tcgtccttca cttgccgaca
     1141 tatcaataac atgtagaata acacgtgtac gctcaatatg tctaaggaac tgagttccta
     1201 atccgacacc ttgactagcc ccttcaatca aaccagggag atcagccatt gcaaagctat
     1261 cacctgattt tgtacgaacc atccctaaat tagggacaat tgtcgtaaaa tgataggcac
     1321 caattttagg ctttgctgct gaaacaacac taagtagcgt cgacttccca actgatggga
     1381 aaccaacgag accaacatca gccaaaattt ttaattctaa ttgtaattca cgttcttcgc
     1441 caggctcccc attttcagca atttcaggag ctggattacg tggggttgca aagcgaatat
     1501 ttccacgacc accgcggcca cctctggcta caacgaattc ctgatcatgc tccacaaggt
     1561 cggtaataac cttacctgta ttagcatcac gaacagttgt tccaggtggt aatgacacaa
     1621 taaggtcttc tgcacctcga ccgtgcatac ctttagtcat ccctttttca ccagcttttg
     1681 ctttaaaatt acggttatat cgaaaatcca tcaaagttcg taaaccttcg tttactttaa
     1741 aaatgaccga tccgccttta ccgccatcac cgccccacgg gcctccattg gggacatatt
     1801 tctcacggcg aaaagcaacc atgccatcac caccgcgacc tgccttaaca ctaattttgg
     1861 cagtatctaa aaacatactc atttctaaaa ttcctattct ttattctatt cagtataaaa
     1921 aaacgcttta cagcgtttct cattacatga ttgcactaat agcaccaaat accaaaccac
     1981 caactgttac tagtaccatc aaaataacaa caaaaagagt tagtttttca aacccagttt
     2041 t
//
LOCUS       DATRSG010000120         2035 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRSG010000120 DATRSG010000000
VERSION     DATRSG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.122"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            176..1417
                     /locus_tag="VBD89_001999"
     CDS             176..1417
                     /locus_tag="VBD89_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO7136471.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            complement(1451..2029)
                     /locus_tag="VBD89_002000"
     CDS             complement(1451..2029)
                     /locus_tag="VBD89_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7136472.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
BASE COUNT          624 a          371 c          371 g          669 t
ORIGIN      
        1 atgtcacctc atttactgaa tatatagtta agtctatatt aacacgaaac gtcttatttc
       61 gcaaactaca aaccgttagc ttaaatatat ctttattaaa tcgcttccat atttccttgt
      121 gctttataag agtttatgat aaattataaa aaatagtaaa tttaaaggag ctagtatggt
      181 tttacaagat ttcgacaacc ttttaaaaaa atatgcccaa ttaattattt ctaaaggttt
      241 aaatgtccaa aaagggcaca ctctcgcttt aacaatcgat gtggaacaag tccacttagc
      301 aaggctttta actgaagtcg cttatgaaaa gggagcaagt gaagttattg ttgattatac
      361 agatgatttt atcacgcgcc agcgactact tcatgcttca gacgaagttc tcacgaatgt
      421 tccacagtat accgttgata aatctttagc gctattaaat aagaaggcta gtcgattagt
      481 tgtgaaatct tctaacccta acgctttcgc tactgttgat cctaaacgtt tatctaaaac
      541 aactagagca accgctattg ccttagagga acaaagtaga gcaatacaag ctaataaagt
      601 atcttggaac gtggctgcag ctgctggtag agaatgggct gcacttgtct tcccagaatt
      661 aaaaacaagc gaccaacaag ttgatgctct ttgggatacc attttcaaat taaatcgtat
      721 ttatgaagat aatcctattg ctgcttggga tgcacatgaa gctaaattat tagaaaaagc
      781 tactagacta aatcaagaac aatttgatgc tcttcattat accgcaccag gtacagattt
      841 aacgcttggt atgcctaaaa atcacatttg ggaggcagcc ggtagtctca acgctcaggg
      901 agagactttt atcgctaata tgcctactga agaaatcttt tcagcacctg attaccgtcg
      961 tgcagatggg tatgtgacaa gtacaaaacc tctcagttat gctggcgtta ttatcgaaaa
     1021 tatgacgttt acttttaaag acggtaaaat tatcaatgtc actgcagaaa aagggcaaga
     1081 aacaatccaa cgcttgatcg aggaaaatga tggggcaaga tcgcttgggg aagttgcact
     1141 tgtcccacat aaaacaccaa tttcactgtc tggactaatt ttctttaata ctttattcga
     1201 tgaaaatgcc tctaatcacc tcgctattgg atctgcatat gccttcaatg tagaaggagg
     1261 aacagaaatg acaagtcaag aattggatga agctggttta aatcgttctt caacacatgt
     1321 tgattttatg attggttcag aacaaatgga tattgatggt attcgtgcag atggaactgc
     1381 tgtcccaatc tttagaaatg gcgaatgggc tatttaaata taaaaaggag gttagtcctc
     1441 ctttttatat ttaataaagt ttcattttct tattgtaaag acttaatgat aacagaataa
     1501 tccctcccgt agatgctatt acagtatctg agtggctatc tcctgtttta ggaagttcct
     1561 ttttaggctt actgtggcct tttttctcat catttgcttt tgaagatgat gattcatcgc
     1621 gatccacttc ttcttgatca ctacttgctt tactaaaatt cttaatacta tcttctgaaa
     1681 ttaatacttt tgttccacta gaaatatcct tattattgcc aatttctgtc ttcgtgctcc
     1741 catcaggctt gttctcttca ctaggtgagg gttgcgatgg ttctgttgta ggtggattag
     1801 ttgagggatt tgtttctggt tcacttgatg aacttgattc attttcagaa gaagaaccag
     1861 tactagactg gtctagttca tctgatcttt tctcctcaga taaggtcgta gtcacgacac
     1921 tcgtgtcagt attcttatca cttgtatcag cataaccgat tgatgaataa gtagcacatg
     1981 ttaagacagt aagtgctacc atcgttaaaa tcttattttt cactttcata ttatt
//
LOCUS       DATRSG010000121         1983 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRSG010000121 DATRSG010000000
VERSION     DATRSG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.123"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            46..531
                     /locus_tag="VBD89_002001"
     CDS             46..531
                     /locus_tag="VBD89_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136473.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
     gene            630..1283
                     /locus_tag="VBD89_002002"
     CDS             630..1283
                     /locus_tag="VBD89_002002"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO7136474.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(1349..1972)
                     /gene="plsY"
                     /locus_tag="VBD89_002003"
     CDS             complement(1349..1972)
                     /gene="plsY"
                     /locus_tag="VBD89_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO7136475.1"
                     /translation="MIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNTFRILG
                     VKAGIVTLTIDILKGTLATLIPIILGVTTVSPFFIGFFAIIGHTFPIFAQFKGGKAVA
                     TSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVGFILTD
                     YDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
BASE COUNT          694 a          328 c          360 g          601 t
ORIGIN      
        1 gctacgtata gttttaccag tcagaaatag taaggatggt tgagtatgag agaaactgaa
       61 acacacgatc accaagcgct aattcaaaaa ttgttagtca gtattcatta tttaaccttg
      121 tttcgtgatg aaattatatt agtagaaaaa accccatctt tactggggaa acatttttct
      181 attgctattg tgcagaatga actaggtgag atactttcta aaatagaagc tttgagtaag
      241 caaaaaaaac tgattcgttc tatttattgg tatgatgaat cgtcatttaa agtcatgaat
      301 aaggctctgg ctattgtaga agaatggatt aagggcttag ataatctttt agaattctgt
      361 caatcacaga cggtttttca agcaatttta ggtgatgaac gtgcacatgt atttggaatc
      421 cttattgatg tttatacttc tttaaacatt ataaatactt ctttgaaaga agaaaattct
      481 agaccagtgt ccttatctga tttggctcta ggtcttaagg acgaagatta ataaaaaaac
      541 gttactgctt tgaatgaata aagcagtaac gttttttata aaatattgct atactagtaa
      601 tacagatatc taaaagtaag gagttataaa tgaaacattc cagttggcat gatttaataa
      661 aaagagaact acccaatcat tactacaata aaattaatac atttatggat gcagtttacg
      721 aaagcggaat cgtttatcct ccaagagata aggtgttcaa tgccatacag ataacaccac
      781 tagaaaacgt aaaagtagtg attattggcc aagatcctta ccatggccct caacaagctc
      841 aaggactctc attttctgtt ccagacaatt taccagctcc tccttcactt caaaatattc
      901 taaaagaatt agcagaagat attgggtcac gttcacatca tgacttaaca tcatgggcgc
      961 aacaaggagt gctgctattg aatgcttgtt taacagttcc agaacaccaa gctaatggtc
     1021 atgctgggct tatatgggaa ccttttactg atgctgtcat caaagtggtt aaccaaaagg
     1081 aaaccccagt agtctttatt ctgtggggtg gctatgctcg aaagaaaaag tcgctaattg
     1141 ataatcctat tcatcatatt attgagagtc ctcatccgag tcccctatct gcttatcgtg
     1201 gtttctttgg tagcagacct ttttctagaa caaatcattt cttagaagaa gaaggaataa
     1261 atgagattga ttggttaaat tagaagggaa atctactctc gaaacattgt tagtcttata
     1321 ataaaaaacc gctagtagcg gttttttatt atttattttt ttgtttactt aggtttaagc
     1381 caaacggaac caaattttct tgcctttttc gaatacgttt gatattatcc tgatgtcgga
     1441 taataatcgt gagggccatt aagataacca cagtggtaaa tatccagtca tagtccgtta
     1501 atataaaacc tactaatgga aaaattaaga cactaaggat acctacgaca gctactgtaa
     1561 tactggatag cgaaatcatg ctaaaaagat aaagcgttaa taaaaagatc actaataagt
     1621 aaaggaaaaa agaaggtgca aatcctagca aaaccccagc gcttgtagca acggctttcc
     1681 cacctttgaa ttgtgcaaaa atagggaaag tgtgacctat aatagcaaaa aaaccgataa
     1741 aaaatggaga tactgtcgtt acacctaata tgataggaat aagtgttgct agagtccctt
     1801 ttaaaatgtc aatagtcaaa gtgacaatgc cagcttttac acctaaaata cggaaggtgt
     1861 tcgtggtgcc agtattacca ctaccgtgtt gacgaagatt gacttggtaa aaatatttac
     1921 caatccaaag cccagtttgg atagagccta acagataggc aatgataatc ataattataa
     1981 tat
//
LOCUS       DATRSG010000122         1907 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.124, whole genome shotgun sequence.
ACCESSION   DATRSG010000122 DATRSG010000000
VERSION     DATRSG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.124"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            316..933
                     /locus_tag="VBD89_002004"
     CDS             316..933
                     /locus_tag="VBD89_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO7136476.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTSLRKKPTQKILSIEELNNK"
     gene            complement(966..1814)
                     /locus_tag="VBD89_002005"
     CDS             complement(966..1814)
                     /locus_tag="VBD89_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO7136477.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
BASE COUNT          650 a          292 c          321 g          644 t
ORIGIN      
        1 tgattttatt ctttttactc ataatgaccc ttaactaata agtttgtttc atatttatta
       61 taacatttaa agatgattat agtaaatttc tactgagtct tagaaataag cttgaatacc
      121 atttcaggga ataatgataa taaatatcgc aacagtatat ttggaatgtt aaattagaag
      181 ttttataaat agctaaatat gaagtgtcct aatgtaatga cttgaaatca tattctggtc
      241 aggtattaaa cgattacttt gacaaggcat ataaaaattt ctatactaaa tttacaaaat
      301 gaaggagagg gaactatgga aatactgatt gcaggtggta gtggttttct agggaagcag
      361 ataataaaag cagcgcttac aaaagggcat aaggtggctt acttatcaag gcatgaaggt
      421 aaaggtgata tatttaagga tcctagatta acctacatta agggagatat tacagaagct
      481 gataagattc atttagaaca tagaaatttt gatatattaa ttgactgtat tggagcgatt
      541 aagcccaatc aactagatga gcttaacgtt aaagcaaccc aaaaagcagt agcactctgt
      601 cacaaaaatc aaataccaaa gttagtttac atttcagcca atagcggcta ttcagcttac
      661 attaaaagta aaaggaaggc agagcagata atcaaagcaa gcggtctgga ttatcttttt
      721 gtaagaccag gtttgatgta tggtgaagag cgacctctct cgattttcca agccaagtgt
      781 ataaaattat ttagtcattt gcctttctta ggtattgttg tacaaaaagt ctttccaact
      841 aaggttgtga tagtggcaga agcaatcgtt acttcgctta ggaaaaaacc aactcaaaaa
      901 atcctttcta ttgaagaatt aaataataaa tagtttatca aaaaagctac tgttaacagt
      961 agcttttaat cttttacttt tccaaaaagt ttctcataag ttttcttctt gtctttatta
     1021 actgcaattt gatttaaatc aagcggttga ttgaatcctg gtagataacc ttgagaggtg
     1081 tattggtgta aatcgtattt aagttcagtt tgtggagctg cttcatagta tccagaatcg
     1141 ctaccataag ttggaatcca aacagcgtca aatcctttta cagagatacc ttgctcagtc
     1201 ataaagtaag taccgatgta gataccaaca tttttagcac caagtctttt taactctttt
     1261 cggaatgctt ggacaccttt attcatgtta gacattgtct cctcttctac gtcaatccaa
     1321 taaaaagttg gtttgtaagg agctgcattc ttataaaata tctgagcctc ttctttcatt
     1381 tctttaacac ttgaaccaag tgcataactg tagacagcta ctgggatatt tcgcttttga
     1441 aattctttga tatgggtctt aaacgattta tcgattccag ttgtataagc agcgttatta
     1501 gtcttagata tctttgatcc accaaagata cgaataacaa cacctgaaat attttttgaa
     1561 agcgtatcgt aatcaatctc cttaggaagt tgccaaccag agacatcgat aatcggttta
     1621 agaacaaact ctttatcttt cgatgatgtt gatttttgcg gtacttttga agcttttacg
     1681 gatggaattg tctttttagc ttcaactaat tcttttttca aagtatttgt cgaatgtaaa
     1741 tgaccaataa ttaataaaag tccaaataaa ctgaaaaaaa ccgcaacaac aataggttta
     1801 attcttcttc tcattattga tattgtaact taaaaggttt aaaaaatcaa agtttacagg
     1861 cttatttacg ttcttttaaa aaaagaggtc gtttatgtaa cattaga
//
LOCUS       DATRSG010000123         1884 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.125, whole genome shotgun sequence.
ACCESSION   DATRSG010000123 DATRSG010000000
VERSION     DATRSG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.125"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            132..938
                     /locus_tag="VBD89_002006"
     CDS             132..938
                     /locus_tag="VBD89_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7136478.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            1021..1524
                     /locus_tag="VBD89_002007"
     CDS             1021..1524
                     /locus_tag="VBD89_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO7136479.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1628..1804
                     /locus_tag="VBD89_002008"
     CDS             1628..1804
                     /locus_tag="VBD89_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO7136480.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT          687 a          261 c          365 g          571 t
ORIGIN      
        1 ttgttattaa aaatcgtcta acagcaagca tagttatcgg tgtaatttac tgaattttta
       61 ttcaaaaaaa cttgacttat gtataaagat gatttatact tgttcctatc ataataaaag
      121 gaagatagtt tatgtcaaaa aaaataatat taggaatttt atctctttta tctgtcgtta
      181 ctttggtggc gtgtggttca tcagacaaac agctacaaga taaagttgag aaaaaaggga
      241 agttagtttt agcggtgagt ccagattatg ctccctttga gtttaaagcg cttgttaatg
      301 ggaaagataa agttgtcggc gcagatattg agttagctca agctattgct gatgagcttc
      361 atgtcaaact agaggtatct cctatgagtt ttgacaatgt tttatcaagt ttacaaacag
      421 gtaaggctga tatggctatt tctggattat catatactaa agagcgtgct aaagtctatg
      481 atttttcaaa accttattac actacagaaa atgcggttct tgttaggaag tctgatttag
      541 gaaaatatac ttcaatcgaa agccttaaag gtaaaaaaat agcagtacaa aaaggaagta
      601 tcgaagaggg agtttctaag aaatcattca aaaattctca cgtcgtgtca ctgacagcga
      661 tgggagaagc gattagtgag ttaaaatcag gcaaggtaga agctgttgat ttagaaaaac
      721 ccgtagctga aggttacttg gcccagaatc cagaccttgt tcttgctaag tttgccctta
      781 aaacaggaga aggggatgct aaggctgtag ctttacctaa agacagtgga aaaatgttga
      841 agactgttaa caaagtgata aagagacttg aaaaagaaga taaatataaa gtttatattt
      901 cagatgcggc taaattaact ggcaatcaag ttgaataata ttaaaaagcc tagtcatagg
      961 cttttttgga tgttataata tggcttgact aagaataata gcataaaggc agaggaatat
     1021 atggttcagg gttcaattaa tattgaaaaa tataaagatt tggcgctcca aaaacaaggc
     1081 gagcatcgta aatttttagc aagtttaaaa aagaaagctc ctaaaaactt agataaaata
     1141 gtccaacaga tacatcaaga ggtttttcaa gaaattgatt gtacagcatg tgctaattgt
     1201 tgtaaaactt tgggtcctct atttacagaa gctgatattt ctaggatttc taaacatttc
     1261 cggatgaagt tatcggcttt tgaagattta tttttagaag tagatgaaga tggagataaa
     1321 gtatttaaag cgatgccttg tccgttttta ggcgatgata acttatgtag tatatacgat
     1381 gtcagaccta aagcttgtcg tgaatttcca catactgatc gtaagaaaat ctatcaaatt
     1441 aataacttaa cgataaaaaa tactcttact tgtccagcgg catacctttt tgtggaaaaa
     1501 ttgaaatcac gtatagggaa ataataaccc agagaaagta tcataaaaat attgacaatc
     1561 tgcttttatt tgggtatcct agtaagtgaa cgtgaaaatg ttacaatatc ttgaggaggt
     1621 gaaacatatg tcaaaaacag tagtacgtaa aaacgaatca cttgacgatg cacttcgtcg
     1681 tttcaaacgt tctgtgacta aagctggtac tcttcaagaa tcacgtaaac gtgaattcta
     1741 cgaaaaacct tctgtaaaac gtaaacgtaa atcagaagca gctcgcaaac gtaaaaaatt
     1801 ctaattagat agaatatgaa aaaacagttc agaattgagc tgttttttca tatattataa
     1861 ctataagtta ataaaaaaac tcgg
//
LOCUS       DATRSG010000124         1842 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRSG010000124 DATRSG010000000
VERSION     DATRSG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.126"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..204
                     /locus_tag="VBD89_002009"
     CDS             <1..204
                     /locus_tag="VBD89_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO7136481.1"
                     /translation="HKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIICRD
                     FGKERANQLFIENAQSILKNHYI"
     gene            213..905
                     /locus_tag="VBD89_002010"
     CDS             213..905
                     /locus_tag="VBD89_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEO7136482.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     gene            916..1605
                     /locus_tag="VBD89_002011"
     CDS             916..1605
                     /locus_tag="VBD89_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO7136483.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
     gene            1618..>1842
                     /locus_tag="VBD89_002012"
     CDS             1618..>1842
                     /locus_tag="VBD89_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136484.1"
                     /translation="MKEKENIQKIIIAMIQTVVVYFSASLTLTLITPNFKSNKDLLFV
                     LLIHYIVFYLSDFYRDFWSRGYLEEFKMVLK"
BASE COUNT          657 a          259 c          323 g          603 t
ORIGIN      
        1 cataaacgct ttaagaaaag agcccgttat tttttagagg aaaatttagt gcattttgta
       61 gcgagtgata tgcataacct tgatgttaga ccgccatttt tagcagaagc ttataagatt
      121 atctgtagag atttcggtaa agaacgtgct aaccaacttt ttattgaaaa tgctcaatct
      181 atattaaaaa accattacat ttaggagatt tcatgaataa aatagctaat acagaagttg
      241 aaataaatat attcaacctt ttaaaaaaat tgtggaaaaa gaaattttta atcacatttg
      301 ttgccattgc gtttgcaaca gctggacttt tctatagtct ttttattgtt acgccacaat
      361 atacttcttc aacaaggata tatgtcatta accctaatac ccctaataat agtattactg
      421 cacaagatct acaagcgggg agttttcttg caaatgacta taaggagatt attacgtcta
      481 ctgacgttct agaaaaagtt atttcttctg aaaaattgaa ttatccttcg tctcagttgc
      541 tacaaaaaat aacagtttct attttaaaag atacacgtgt tatttcaata tcggtcgaag
      601 atgctaatcc aaaaatgtct caaaaattag caaattcagt tagagaagca gcagtttcaa
      661 aaatcaaggc agttactcaa gtagaggata tcactactct tgagaaggga aatttaccta
      721 aagcaccatc ttctcctaat attaaaaaga atgtactaat cgggtttatt gttggtgcag
      781 gattatcaac tattgtttta gttattatgg ggattttgga tgatcgtgtt aatactgaag
      841 aagatattga aaaggtctta ggacttactt ctttaggtat agtaccagat ttgaataaac
      901 tttaaggaga atataatgac tcgtttagaa atagttgata gcaagttaag acaagcaaaa
      961 aaaacagaag aatacttcaa tgcgatccgt acaaacatac agtttagtgg aaaggaaaat
     1021 aaaattcttg caattgcctc tgttagggaa ggggaaggaa aatccactac ttcaacaagt
     1081 ttagctttat ctttagctca agcaggattt aaaacattat taattgatgc ggatactagg
     1141 aactctgtta tgtctggaac ctttaaagca actggaacta ttaaaggctt gacgaattat
     1201 ttatcaggta atgcagatct tggagatatt atctgtgaaa ccaatgttcc tagactgatg
     1261 gtcgttcctt caggaaaagt accaccaaat ccaacagcat tacttcagaa cgcttatttt
     1321 aataagatga ttgaagctat taaaaatata tttgattata ttatcatcga tactccacct
     1381 attggtttag ttgttgatgc cgcaataatc gctaatgctt gcgatggttt tattttagta
     1441 acccaagcag gcagaataaa acgtaattat gttgaaaaag caaaagaaca gatggaacaa
     1501 agtggttcaa agttcttagg tattattctt aataaagtta atgaatctgt tgctacttac
     1561 ggcgattatg gaaattacgg aaaaagggat agaaaaagga agtaagggac tctggtattg
     1621 aaagaaaaag aaaatataca aaagattatt atagcgatga ttcaaacagt tgtagtttat
     1681 ttttctgcaa gtttgacatt aacattaatt actcccaatt ttaaaagcaa taaagattta
     1741 ttgtttgttc tattgataca ttatattgtt ttttatcttt ctgattttta cagagacttt
     1801 tggagtcgtg gctatcttga agagtttaaa atggtattga aa
//
LOCUS       DATRSG010000125         1710 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRSG010000125 DATRSG010000000
VERSION     DATRSG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.127"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            500..>1710
                     /locus_tag="VBD89_002013"
     CDS             500..>1710
                     /locus_tag="VBD89_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136485.1"
                     /translation="MERLKSSGLSMGKPVDYMGVNGIPIYTKTLSIDNKFAFENNSKD
                     SSYSSNINISEDKIKENDQKILDLIVKSGANNQNLTDEEKVIAFTKYIGEITNYDNEA
                     YRARNVDTEYYRASDLFSVTERKLAMCVGYSVTAARAFNIMGIPSYVVSGKSPQGISH
                     AAVRAYYNRSWHIIDITASTYWKNGNYKTTYSDFIKEYCIDGYDVYDPAKTNNRFKVK
                     YMESNEAFENWIHNNGSKSMLFINESAALKDKKPKDDFVPVTEKEKNELIDKYKKLLS
                     QIPENTQNPGEKNIRDYLKNEYEEILKKDNLFEHEHAEFKESLNLNESFYLQLKKEEK
                     KPSDNLKKEEKPRENSVKERETPAENNDFVSVTEKNNLIDKYKELLSKIPENTQNPGE
                     KNIRNYLEKEY"
BASE COUNT          759 a          202 c          268 g          481 t
ORIGIN      
        1 tttaaaaaat taagaaaaaa gattaaaatt aacttgtgtt ttatcatttg ctgtagcttc
       61 aacattattc aacctatctg atttaaatat atcatatgct aatcaaaata atattcaaga
      121 aactaatcta gttgaaaaaa acagtgaaga taagtttatt caagagttaa ataggtataa
      181 aacagagata ccaaatttca aaggttttaa tgtttggatt ttaggtgata aaggttatta
      241 caaaaacctt atcaatttag aagaaattaa aaatatccaa gctacattaa aaaaagaaag
      301 aaatgaagaa tatgtctttg tcaaattaaa tgggaaaatc gcacatgata caacagtatt
      361 cttgatgaat aaaaaacata aattacttaa aaatatagag gaatttaaga cgataaccca
      421 aaaaaggcta actgagagag gaaagttccc atatgatacc gttcattcta cgtttgaaat
      481 taaggatgaa aactttatca tggaaagatt gaaatcatca gggttatcta tgggtaaacc
      541 agttgattat atgggcgtaa atggtatccc tatttacact aaaacattat ctatagataa
      601 taagtttgcc tttgaaaata attcaaaaga ttcttcatat tcttcaaaca ttaatattag
      661 tgaagataaa ataaaagaaa atgaccaaaa aattctagat ttaatagtta aatctggagc
      721 taataatcaa aacttaacag atgaagaaaa agtaattgct ttcactaaat atataggtga
      781 aataactaat tatgacaatg aagcttacag agctagaaat gtcgacacag aatattacag
      841 agcttcagat ttattttctg taacagaaag aaaacttgct atgtgcgttg gctatagtgt
      901 aacagccgct agagcattta atattatggg gatacctagt tatgtcgttt ctggtaaaag
      961 tcctcaagga atatctcatg cagctgtgag agcgtattat aatagaagtt ggcatatcat
     1021 agatatcaca gccagtacat attggaaaaa tggtaattac aaaacaacgt attccgattt
     1081 tattaaagaa tactgtatag atggttatga cgtttatgat cccgcaaaaa caaataatag
     1141 atttaaagtt aagtatatgg aatcaaatga agcgtttgaa aattggatac ataataatgg
     1201 aagcaaatct atgttattta ttaatgaatc tgcagctcta aaagataaaa aaccaaaaga
     1261 tgattttgta ccagtaacag aaaaagaaaa aaacgagctg atagataagt ataaaaaatt
     1321 attgtcacag attcctgaaa acacacaaaa tccaggagaa aaaaacataa gagattattt
     1381 gaaaaatgaa tatgaagaaa tattaaagaa agataattta tttgagcatg aacatgcaga
     1441 atttaaagaa agtctaaatt taaatgaatc attctaccta caattaaaga aagaagaaaa
     1501 gaaaccgagt gataatctca aaaaagaaga aaaaccaaga gaaaattcag taaaagagag
     1561 agagactcca gctgaaaaca atgattttgt ttcagtaaca gaaaaaaaca acttgattga
     1621 taaatataaa gaattattat caaaaatccc agagaataca caaaatcctg gggaaaagaa
     1681 tatacgaaat tatctagaga aagaatatga
//
LOCUS       DATRSG010000126         1705 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRSG010000126 DATRSG010000000
VERSION     DATRSG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.128"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            152..1507
                     /gene="rlmD"
                     /locus_tag="VBD89_002014"
     CDS             152..1507
                     /gene="rlmD"
                     /locus_tag="VBD89_002014"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7136486.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDIIRQ
                     ALMKFKPEGYENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDRLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     V"
BASE COUNT          575 a          225 c          347 g          558 t
ORIGIN      
        1 tctagctcta gagtgtaaag tctttttttc ttttcgattt tggttatttt catagttttt
       61 attatagcaa aaatgataaa atagtaaaaa tgagtctttt tttaaatgtt atggatttat
      121 agtattttct tagttaggga gtaatgaact gatgaatgtg gttttaaaac agcgaattcc
      181 tttaaaaatt aaacgaatgg gaattaatgg tgaaggaata ggtttttata aaaaaacact
      241 aatctttgtt ccaggggctc tcaagggaga agaggtgttt tgtcagattt cttctgttag
      301 acgtaatttt gcagaagcta aactgttaaa gataaataaa aagtctaaaa atagggtaga
      361 tcccgcttgt tctatttata aagaatgtgg gggatgccaa ataatgcacc ttcaatatga
      421 taagcagcta gagtttaaaa cggatattat tcgacaggct ctaatgaaat ttaaaccaga
      481 gggatatgag aattatgaaa tccgtaaaac gataggtatg tcggaacctg aacactatcg
      541 tgctaaatta caatttcaag tgcgttcgtt tggagggaat gtaaaggcag gtttatatgc
      601 acaagggaca catcgattga ttgatatcaa agattgttta gtgcaagata ggcttactca
      661 ggaaatgata aatcgagttg cagaactttt aggaaaatat aaattgccta tttataatga
      721 acgtaaaata gctggtgttc gtactgttat gattcgacga gcacaagcga gtggagaggt
      781 tcaactgatt tttatcacaa gtaaaagact tgattttgat gatgttgtta ttgaattagt
      841 tcgagagttt cctgagttaa agacagttgc tgtaaatatc aacgctagta aaacaagtga
      901 tatctatggt caaataacag aagttatttg gggtcaagaa tccatcaatg aagaagtatt
      961 agattatggt ttttcgttat caccacgtgc tttttatcaa cttaatccta aacaaacaca
     1021 aattttatat agtgaggcag tcaaggcctt agatgtgaag gaagatgatg atttaattga
     1081 cgcttattgt ggagtaggaa cgattggact tgcttttgct ggaaaagtta aaagtgttcg
     1141 tggaatggat attatccctg aggctattca ggatgctaaa gagaacgcac ttcatatggg
     1201 ttttactaat acacattacg aagctggaaa ggcagaggat attatcccta ggtggtatag
     1261 tgagggattt cgcgctaatg cgttaatcgt ggatcctcct cgcacaggct tagatgataa
     1321 attgctaaat actatactaa aaatgcctcc agaaaaaatg gtttacgttt cttgtaatac
     1381 atctacgctt gctagagact tagtaacatt aacaaaagta taccatgttc actacatcca
     1441 atctgttgac atgtttccac atacagcaag gaccgaggct gtagtgaaac tacaacgtaa
     1501 ggtataataa agttttctaa tctaaaaata agagtatttt tgggtattct tatttttaga
     1561 tagtaatggt gagatattaa tatagcatag tataaaacac atggtttaag ctatttgata
     1621 cggaaattta ccttagaaat ggtaaataat tccattaagt atattcatct ttgcaaattt
     1681 gaccttgcct tttaagtttc attgg
//
LOCUS       DATRSG010000127         1704 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRSG010000127 DATRSG010000000
VERSION     DATRSG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.129"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            59..1411
                     /locus_tag="VBD89_002015"
     CDS             59..1411
                     /locus_tag="VBD89_002015"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO7136487.1"
                     /translation="MKVVKFGGSSLASSQQLYKVLNIIKSDYTRRFIVVSAPGKRYEE
                     DLKMTDALIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLAS
                     LPIENNQFLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARII
                     PGSYDKIENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENF
                     TDVDGIFAAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPQQPGTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDL
                     NISFEHMPTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGE
                     NMKSQIGVTATATQALSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK
                     "
     gene            complement(1627..1700)
                     /locus_tag="VBD89_002016"
     tRNA            complement(1627..1700)
                     /locus_tag="VBD89_002016"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1664..1666),aa:Asn,seq:gtt)
BASE COUNT          603 a          300 c          289 g          512 t
ORIGIN      
        1 atgacaaatt ttctgacttt tgttataatt tatctaaaat tcattcttga ggtaacctat
       61 gaaagtagtc aaatttggag gaagctcttt agcatcttcc caacaattat acaaagtact
      121 aaatattatc aaatcagact acactagacg gtttattgtc gtttcagctc cagggaaacg
      181 ctacgaagaa gacttaaaaa tgacagatgc tttgatacaa tattatcaaa attatattaa
      241 cggtaaagat attgttaaag accaaacttg gattatcaat cgttatcaag aaattattag
      301 tgatttgtct ttaggaagca ctattgcaga agaaattact cgctctatag agcagctagc
      361 ttcacttccc atagaaaata atcagttttt atatgattgt tttttagcag ccggtgaaga
      421 taacaacgca aagttagttg caacgttttt taatcaaaat gacattcctg caagatatgt
      481 tcatccaaac gaagcaggaa ttattgtaac taaagaacca tgtaatgcac gaattattcc
      541 aggaagttat gataagattg agaacttatg tctatacaat gaggttcttg ttatccctgg
      601 attttttgga gtcacagaag ataaccaaat ttgtaccttt tcaagagggg gatctgacat
      661 tactggatcc ctaattgcag caggcataaa agcagacctt tatgagaact tcacagatgt
      721 tgatggtata tttgcagcgc atccaggtgt agttaagaac cctcacgcta tccctgagct
      781 tacttataaa gaaatgcgtg aattagccta tgcgggtttt tcggttttac atgatgaagc
      841 tttacttcct gcctatcgtg gcagaatccc tcttgttatt aaaaatacaa ataatcccca
      901 acagcctggt acaaaaatag ttttaaagca tactcgtagt aacatagcag taactgggat
      961 cgcttctgat agccgttttg ctagcataaa cgtatctaaa tacttaatga atagagaagt
     1021 aggtttcggc cgaaaagtac tacaaatttt agaggattta aatattagtt ttgaacatat
     1081 gccaactggc atagatgatc tatccattgt cttacgtgaa aaagaattga caccaatcaa
     1141 agaacaagaa atcttaaatt acctaactcg taaactagaa gtagattacg ttgacatcca
     1201 acacaattta tctacaatcg taattgtagg tgaaaatatg aaaagtcaga ttggagtcac
     1261 tgcaacagcg acacaagcct tatcaagaga aaaaatcaat atcaccatga tatcacaagg
     1321 ttcaagcgaa gtctccatta tgttcgttat agacagtaag gatgaaaaaa gagctattaa
     1381 agcactatat gaaacattct tccaaaaata gtacctatta cactacttac actattagat
     1441 agataacaaa tcgtcctaag taagtttact taggacgatt ttatttagaa cataggatag
     1501 ttttttcaat tttaatcgta actacttggt atcactgaca aattcggaca attaagatgc
     1561 tagccgacct taaggaataa taattccaat aaaaaaaggc taaccaaagt tagtctcctt
     1621 tatctactcc gccagtagga ctcgaaccta cgacatcatg attaacagtc atgcgctact
     1681 accaactgag ctatggcgga ttat
//
LOCUS       DATRSG010000128         1603 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRSG010000128 DATRSG010000000
VERSION     DATRSG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.130"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..325
                     /locus_tag="VBD89_002017"
     CDS             <1..325
                     /locus_tag="VBD89_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136488.1"
                     /translation="PIPSELTFIKFYGHGLGEADYSYFQSIFDSINLYSSDTVLIFYW
                     SCYNENIREQIKSQQVHAVHKLIEKYGDTFNNKNHGRNLFTKLQLENRLIIQEILYND
                     ILNPY"
     gene            complement(460..825)
                     /gene="rplL"
                     /locus_tag="VBD89_002018"
     CDS             complement(460..825)
                     /gene="rplL"
                     /locus_tag="VBD89_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO7136489.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(889..1389)
                     /locus_tag="VBD89_002019"
     CDS             complement(889..1389)
                     /locus_tag="VBD89_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO7136490.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1419..1552)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          494 a          302 c          250 g          557 t
ORIGIN      
        1 tccaattccc tctgaactaa cttttataaa attttatggt catggtctgg gtgaagcaga
       61 ctattcttat tttcaatcaa tttttgactc aattaatctt tattccagtg acactgtact
      121 aattttctat tggagttgtt ataacgagaa tattagggaa caaattaaat ctcaacaagt
      181 tcatgcagta cataaattaa ttgaaaaata tggagatact tttaataata agaatcatgg
      241 cagaaattta ttcacaaaac ttcaattaga aaatcgttta attattcaag aaatacttta
      301 taatgatatt ctaaatccat attaaacaaa acacccccac aaaatattgt gggggtgttt
      361 gtggggaaga ttcaattgaa tctttccttt tttaatcgcc ttaactttaa cgaaaagact
      421 ttaaaactag gctttcacct ttaaacaacc aatagatgat tatttaagag taactgaagc
      481 acctgcagct tcaagttttt ctttgatttc gttagcttct gcttctgaag caccttcttt
      541 gataactgat ggtgcgttat caacgatagc tttagcttct ttaagacctt cgcctgtgat
      601 ttcacgaaca actttgataa cgccaacttt tttgtctcca gcagctgtta attcaacgtc
      661 aaatgagtct ttagcagcag cagcttcacc accagcagcg gcagcagcta caggagcagc
      721 agcagttaca ccaaattctt cttcgatagc ttttacaaga tcgttaagtt caagaattgt
      781 agcttcttta atttcagcaa taatattttc aatgtttaat gccattgtta tttcctccaa
      841 aaatatgttt taatataaaa ttatgtagct aggctacttg aagagtgctt atgccacttc
      901 ttcgtctttt tctgcaactg ctttgacagc atatgcaatg ttgcgtactg gcgcttgaag
      961 tactgaaagg agcattgaaa gcataccttc tttgtttggt aatttagcaa gagcgttaat
     1021 ttcttcaact gaagtgaatt taccgtctac tgaaccacct ttgatttcaa gagcttctgc
     1081 atctttagcg aaatcgctaa taactttagc tggtgcaata acatcttcgt tagaaaacgc
     1141 aactgctgat ggaccaacaa aaagttcttt aaggtcttca agacctgctt tttcagctgc
     1201 acgagttaaa attgagtttt tgataacttt aaactcaaca tcgctttcac gaagtgaacg
     1261 acgtaagtta gtatcttgct caacagtaag accacgtgaa tcaacaacaa cgatacttgc
     1321 tgctgctttc attttctcag caataagttc aacttgttca gctttttttg caataattgc
     1381 ttcactcatt agttttacct ccgtttttat tttttgggat aggcacaaaa aaatcgcacc
     1441 taaacctaga cacgaaagta caatacgtta ttttataatg acgttttgtg cctcggtagg
     1501 aatattatga gcactgctcc cctactgtct taggtcagtt tcatcagaaa ctttaaatag
     1561 tataacttat aattctagca ttgtcaataa tttttattat ttt
//
LOCUS       DATRSG010000129         1588 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.131, whole genome shotgun sequence.
ACCESSION   DATRSG010000129 DATRSG010000000
VERSION     DATRSG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.131"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            184..593
                     /locus_tag="VBD89_002020"
                     /pseudo
     CDS             184..593
                     /locus_tag="VBD89_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            668..1348
                     /locus_tag="VBD89_002021"
     CDS             668..1348
                     /locus_tag="VBD89_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7136491.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYMSFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFERWLDDSNFDVNEYQIRSL
                     SSIEQKTK"
BASE COUNT          607 a          178 c          291 g          512 t
ORIGIN      
        1 aagtggttta tagataatta aggaaggaat attgattaga aatgaataat ttggatacac
       61 tatattgtaa tttagcaatg tgcaatcata gtactaatgg aaaggtagcg acttcagctt
      121 gttgatttat ctaatgtaaa tgagttttta gaattttcat ctgattttga aactgtattt
      181 catatgcaac gatataaagc caataccaca gaagaagcac attgattttg gcgaatgtct
      241 gtatgaaatc attattagga atctatgcga tgattgaaac agaatcaaat aaaatgattg
      301 gtattataga attagaaatt agagacgagt ttccagcaga attcggttat atattaaata
      361 aaaattatag tggaaaaggt tatatgataa aagcttgttc aaaattgatg tcaattggat
      421 ttgaacaact agatttggaa agaatttatg ctcgatttga actaataata aaaaatctga
      481 aaatgttatg gaatgcctaa gaatgcaaaa agaaggtgaa cgtcgtcata ttgtaaaaaa
      541 atccaaaagg tgagtggaaa accagagcat attattctat tttaaaggaa taatatttta
      601 aatagacgac aataaaaatt tgtgaaagca gaaaaattaa atgagataca ataggtttaa
      661 tcaactagtg ggtgatgtcg ttgaaaattt tacggaaggt aaaaatccga agattgatac
      721 acttaatggg aaaacagtta gaatagaaaa aataaatcca gatcattttg aagacttatt
      781 tcaagtttat ggagaattgt ctactgaaga tagtttaact tatatgtctt ttagcaaatt
      841 taatagtaag aatgaatttg atgtcttttt ccaaactcta ttaaaatcag aagatcctta
      901 ttatttagct attgttgata ataatactgg taaagcatta gggacttttt cattaatgcg
      961 tattgataca aaaaatagag ttgttgaaat gggatgggtg gtttattcat caaaactaaa
     1021 acaaacaagg atagcaacag aagctcaata tttggttatg aaatatgttt ttgaagaact
     1081 ctgttaccgc cgttatgagt ggaaatgtga tagtttgaat gcaccatcta ataattctgc
     1141 aaaacgactt ggttttactt ttgaaggaac ttttagacaa gctgttgtat ataagggtcg
     1201 taacagagat acaaattggt attctatttt agataaagaa tggccagaga aaaaaacacg
     1261 gtttgaaaga tggctagatg attcaaactt tgatgtaaat gagtatcaaa tcagatcact
     1321 atcaagcatt gagcagaaga caaaatagag tcattcatta atcactaaaa tttcaaaatt
     1381 acatgttttt ttagctaaca tcttaaactt tgttaaataa atgtttatta acttttaaag
     1441 gtgcatggta ggactcaaaa tcgggtgtcc tcacggacgt gctagctcga ctttggtcgc
     1501 gggcatcaaa gaaaaaagtc aagattatta tcttgtcttt ttttctatga gttattagct
     1561 gtaaaagtta cgtaaaatta ccaaacaa
//
LOCUS       DATRSG010000130         1530 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRSG010000130 DATRSG010000000
VERSION     DATRSG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.132"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            7..237
                     /locus_tag="VBD89_002022"
     CDS             7..237
                     /locus_tag="VBD89_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136492.1"
                     /translation="MAFEPKNSELTKVLKESLDEEKKEIFSSEMNIRDFERTKQYQFT
                     LQPSVRKKIDRLSKEKGYRSASSFINDFFKNL"
     gene            334..882
                     /locus_tag="VBD89_002023"
     CDS             334..882
                     /locus_tag="VBD89_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136493.1"
                     /translation="MIIIVSSVINLFTGSESSNTSKKNKTNPSGATSAKVVKHKKTDE
                     ERAEDYIKTLGLNPSDYIKEMDYSKVDFRGDLSSVSGNYNNFTVENINQTFEKGDKSL
                     KDIIEIFGKPDTIWVNYVDNQYNTDLYWKKDDTDDSNYIHAEWSEPKDEYDVQSLSGT
                     ASNSGTGKFQSFIGLDGFEPAQ"
     gene            990..>1530
                     /locus_tag="VBD89_002024"
     CDS             990..>1530
                     /locus_tag="VBD89_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HEO7136494.1"
                     /translation="MEVKKFSEKDFVNEINKIKQKQFLSQIEQYESYIAPQMRTKGYK
                     RINQSERTVVFSFGEITFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLAT
                     MMPYRQVCKVIDSTLQTIITKDCVLKAVKFVEKLLKEKERYRFYLEEPPERKKVKKLY
                     VEGDGVMIKSTDSREERRYL"
BASE COUNT          616 a          172 c          282 g          460 t
ORIGIN      
        1 atgaatatgg catttgaacc aaaaaattcg gaactaacaa aagttctaaa agaaagttta
       61 gatgaagaaa agaaagaaat tttctcatct gaaatgaata ttagggactt tgaacgtaca
      121 aaacaatatc aatttacact tcaaccttca gtccgaaaaa aaattgatag gctatcaaaa
      181 gaaaaaggct atcgttcagc atcatctttt ataaatgact tttttaaaaa tttgtaaaca
      241 aagtttgaat tatatttatt atggaaatca ttattagatg ggaaaaatgg ttaagaaaaa
      301 agtggttgtg gtggatgttt aagtttaata atagtgataa ttattgttag tagtgttata
      361 aatttattta caggttctga aagcagtaat acttctaaaa agaataagac aaatcctagt
      421 ggtgcgacaa gtgcaaaagt cgttaaacac aaaaaaactg atgaagaacg agcagaagat
      481 tatataaaaa cgttaggatt aaacccgagt gattatataa aagaaatgga ttattcaaag
      541 gttgatttta ggggggattt atcatcagta tcaggtaatt ataataattt tactgttgaa
      601 aacatcaatc aaacttttga aaaaggtgat aaatctttaa aagatattat tgaaattttt
      661 ggaaagcctg atacaatttg ggttaattac gttgacaatc agtacaatac tgatttgtac
      721 tggaaaaaag atgacacaga tgattctaat tatattcatg cagaatggtc tgagccaaaa
      781 gatgaatatg atgttcaaag tttaagtggc acggcttcaa attcaggtac tggcaagttt
      841 cagtcattca ttggattaga tggatttgag cctgcacagt aggataaata gaaagatcac
      901 gttattaaat gtggtctttt actttaactt ttcgaataaa aaatagcaat aatattctaa
      961 tcctgtaaca aaaattgtaa aatattatca tggaagttaa aaaattctcg gaaaaagatt
     1021 ttgtaaatga aataaataaa ataaaacaga aacaattttt aagtcaaatt gaacagtatg
     1081 aaagctatat cgctcctcaa atgagaacga aaggctataa gaggatcaat cagtctgaac
     1141 gtacggttgt tttctctttt ggagaaataa catttagtag gagtcgctgg acaaatggct
     1201 ttgaaactag aataccagta gatgagtggt taggtcttga aaaatataag agatattcaa
     1261 tagaattctt atatcatgtt gcaaaattgg ctacaatgat gccttatcgt caagtttgca
     1321 aagtaataga tagcactttg caaacaatca taacaaaaga ctgtgtttta aaagcagtaa
     1381 aatttgtaga aaaattgtta aaagaaaaag aacgctatcg tttttatttg gaagagccac
     1441 ccgaacgtaa aaaagtgaaa aaactgtatg ttgagggtga tggagtcatg attaaaagca
     1501 cagattctag agaggaaaga aggtatttag
//
LOCUS       DATRSG010000131         1521 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRSG010000131 DATRSG010000000
VERSION     DATRSG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.133"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            11..451
                     /locus_tag="VBD89_002025"
     CDS             11..451
                     /locus_tag="VBD89_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein BUG3"
                     /protein_id="HEO7136495.1"
                     /translation="MKKHIKSIIPIVLIGMILGGCQMNSEHKSQYNETKSSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDFVKSQNKKIESVQIDWNDVRWSKGGNGTPQGGGEGI
                     LLFGEINNDSESSWRVDIDIEKGRLDLKNMYLGQPIRIGGKLFE"
     gene            444..788
                     /locus_tag="VBD89_002026"
     CDS             444..788
                     /locus_tag="VBD89_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136496.1"
                     /translation="MSNWNLESFDNLFATLAESTYTKRPSNFTYNELNPKLLRQLNSG
                     SSVELDFSKSVKKGKKVIEGGQNLPNDGIVYLQPDKSLKSIDEKVEVRVPDVNGGYHS
                     EHCVTNSYQKGY"
BASE COUNT          545 a          189 c          263 g          524 t
ORIGIN      
        1 ggtgcaaata atgaaaaaac atataaaaag tatcatacca atagttctta ttggtatgat
       61 actaggaggc tgtcaaatga atagtgaaca taaaagtcag tataatgaaa caaaaagtag
      121 caagcaatca gaagtgaaga aagataaaaa aatgacaaaa aaagaacaat tagcttatct
      181 caaagagcat gaacaagaaa taattgattt tgtaaaatct cagaataaaa agatagaatc
      241 tgtacaaatt gattggaatg atgttcgatg gagtaaaggg ggaaatggta cacctcaagg
      301 aggaggagag gggattttac tttttgggga gattaataat gattctgaat caagttggag
      361 agttgatatt gatatagaaa aaggacggct agacctaaaa aatatgtatt taggacaacc
      421 tatacgaatt ggaggtaaat tatttgagta attggaactt agaaagtttt gataacctat
      481 ttgctacttt agcagaatca acatacacaa aaagaccaag taactttaca tataatgaat
      541 tgaatcctaa gttgctacga caactgaact caggtagttc tgttgaattg gatttctcaa
      601 aatctgttaa gaaaggaaaa aaagttatcg aaggcggtca gaacctcccc aatgatggta
      661 ttgtctacct gcaaccagat aagtccttaa aatctattga tgaaaaagta gaggttcgcg
      721 ttcctgatgt caatggcggt tatcactctg agcattgtgt tactaattcc tatcagaaag
      781 gttactgaaa gatgatgaag cagtggctct ataatcacta aaagctcatc attgctcagt
      841 gtttaagtta acttctaaag ctcgaacctt gttaaatcaa ggtttgttta cttttaaaag
      901 tgacatttta tttttctaaa aataaggtta tcgggattcc tcattctaag ttactttgtt
      961 tatatgtacc tttacttaag gtttattttc attattttta atttaatatt ttggagtatg
     1021 tacgctccat tatagtattt tcgaagacat ctttaaaaga actgtagaag ttaaaatatt
     1081 ttaacctagt atctaacgtc atatattggt acatagtttt aaatatcctt ttgataacct
     1141 tgtacatatg aaataggcta aatattttta gtgaactaaa tgtaagttta ttgtttgtat
     1201 taggcgagat aacaatattg ttgtttttat tctttctgag taatggttta ttaattatgt
     1261 aattattctg gtatcaaatg atacttttaa tattccagtt tgtcaataaa tcatcaatgt
     1321 aattcttttc atcaaaagcg atccctttat ctggttgatt taatatgtta acttgatgat
     1381 tattcttctt gcttaatata tgggataata attatcttaa attgacactt ggagcattca
     1441 atcacgtgat tgtaataact aaaaattgtt taaaatttaa ggatgtattt cttgttgatc
     1501 ttactatctg atctaataat g
//
LOCUS       DATRSG010000132         1490 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRSG010000132 DATRSG010000000
VERSION     DATRSG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.134"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            157..1440
                     /locus_tag="VBD89_002027"
     CDS             157..1440
                     /locus_tag="VBD89_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7136497.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
BASE COUNT          534 a          266 c          243 g          447 t
ORIGIN      
        1 agtataactt agtgtcgtag agattaattc ctatcattca ctgctttatt ttaggataac
       61 taaacaattt attgtttaaa atgaaaggat ttatgaatgt ctaggaaaac atttaaacgt
      121 atactatcga ttggagtttg cacgctcgta ctatcgatga gtctttatta cactgaaaaa
      181 gcccacgcta ttgctggtcc tagtgaccgc caatacgtag aaaacccaaa tcctcacatt
      241 attgtaaatg ttacaggtac tgatcaaaac ggaaatagca ttttaccgca ttatatcgaa
      301 gtcaatgtaa agatgggaca aactttaagt aaagaagaaa ttctagatta tattgctcga
      361 aatttaaact ctagtgttgg aggagaaagt aaaaacgttc aatacagcaa catcgagttt
      421 aaggaaagtg cttatctgaa gcgtcaatta gatgatggca agacagaaga aatagcaatt
      481 gataacgacg gtgttactgt acctaaagac ggtccaaaca aattttggat tgacgttcca
      541 gtaacttgta ctgttactcc tatcgtaaca gaaacacatg aagttcgatg ggggactcca
      601 gtcgctatat cacaccgtat ttactttgtt gaagaatctt ctggaaaagt tttagatgaa
      661 tacacaaatc tacacactgc tgattcggaa cttaacggtt atcgtgttgg agattatatc
      721 acagactatg cactttctaa gtctgcttac gaagcttttt taaactctcg tttagataaa
      781 gaaggttaca aacttcaaca tcgtattagc acgaatgtac gacaaaacct tcaaattaat
      841 aaattgattt tcaattatga ctttaatgag gaaaatattt actaccaaat cgggaatatc
      901 cgtccactat taagtcgctc atcagctgaa gtagaatctg acatcattac agaacgctac
      961 tatgtttcta aaaatgctaa aagtttagca cgtacagaat caaccatttc gattaaaatg
     1021 gttgatgcca aaactgaaca accgctattt aaccacacat taactggtta tcaattggta
     1081 actgtctccc atgtctataa cagactcttt gaagaaaatc ttatcccaac tacaaaatca
     1141 ggagaaagat attttattca aaatatgaaa aaaacagctg aacaagaata tactgtttat
     1201 ctttcagaaa caccttattc taaagagaac gctcccgtaa tttcttatga tgcaagacct
     1261 gttgattggg attatcactc aggcgcttca ggatcacttg aaaatcagcc taacatctat
     1321 actgaagaag attcaactga atttttgggt aataaaccac aagcagcttg ttatccaaac
     1381 aaacaatttg cttgcgaaaa tactgactct aaatacaact atagctattt agaaaaatag
     1441 ttataaccga tagggttaat ctctcattat cttatactga ttaaaatcat
//
LOCUS       DATRSG010000133         1453 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRSG010000133 DATRSG010000000
VERSION     DATRSG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.135"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1338)
                     /locus_tag="VBD89_002028"
     CDS             complement(<1..1338)
                     /locus_tag="VBD89_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO7136498.1"
                     /translation="MKKESISIKDILWFIIPSLFGVLLLMTPFKYNGMTTVAVSVISK
                     TINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIR
                     LIGLALGLMTVLHIGPEMVCGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEF
                     VGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTT
                     FSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPK
                     KETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGT
                     IALIISVSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSE
                     MTRFIVATVSIVQLIYMSETGAVILGSKIPINILELFIIFIERTIISLPIIVLM"
BASE COUNT          558 a          265 c          227 g          403 t
ORIGIN      
        1 catcaaaaca ataataggta atgaaataat tgttctctct ataaaaatga taaataattc
       61 tagaatgttg atagggatct tggaacctaa tatcaccgca ccagtctcag acatataaat
      121 taattgcaca atagaaactg ttgcgacaat aaaccttgtc atctcactgt gaatttcagc
      181 cgccattatc gaaggaacaa ccatatctga aaaaccaaca atcatggttt gactcgcaat
      241 atctgcttct ggaatcccta aaagttcaag gaacggaagg aatggtgtac ctaaaatttt
      301 aaaaataggt gttgatactg aaataatcaa agctattgtt ccgacagtca taattgttgg
      361 cataacgcca aaccataaac taagtactgt cttagaggca ttattgacaa aagtcttcat
      421 gtttccattt gcttttgatt tatcgatggc aagcttaagt ccccactcta tcttgttata
      481 accatccgga attacctcag atgtttcttt ctttggttta gtgtaataac tatttgattg
      541 acgagataag ggagggatat aagggataat gaatgcacag acaacactgc taatgatgac
      601 tgttagataa aaatggccaa aataatcagt taagccaaca ttctgcaaaa caacaagaca
      661 aaaagtgatt gatactgctg aaaaagtagt ggaaatcgtt gcagcttctc tactggtata
      721 ataaccctca acatactgct tatcggtcaa agcaattcca atagtaccat cacctacaaa
      781 tgaggctaca caattaactg ctgaccgacc aggtagctga aagaatggac gcataatggg
      841 agttaggaag acaccaacaa actccagtag accaaattct gtcaaaaatg gaagaataaa
      901 acccgcagca aggaaaatgg taaacaaacc accaatcaaa tcaaaaagga taaggccgcc
      961 tgtctctttc ccacatacca tttcaggacc aatatgtaaa accgtcatca gaccaagtgc
     1021 caaaccgatc aaacgaataa tcagccagaa aatggtaatg tcagaaattt cttttagcaa
     1081 gtcatttttt tcaataaagc taggtctaaa cagacgataa caaagtgcta acacacatga
     1141 aataaaaatg attagtaaaa taatataatg tataggaaaa acagcgttaa tccattgatt
     1201 aattgtctta gagattacag agacagctac tgttgtcata ccattatatt taaatggagt
     1261 cattaataat aatactccaa ataacgacgg aataataaac cacaagatat ctttaataga
     1321 tatactttct tttttcatac aatcctcctg gataatatgg aattgtttct aaatatactc
     1381 ataacttcca ttgtaaaatt ttataataaa aaaaacaacc ttgaattaaa gaaaatttta
     1441 cttagtaata ctt
//
LOCUS       DATRSG010000134         1398 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRSG010000134 DATRSG010000000
VERSION     DATRSG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.136"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            387..779
                     /locus_tag="VBD89_002029"
     CDS             387..779
                     /locus_tag="VBD89_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEO7136499.1"
                     /translation="MQSQKLNILQRVLRDKFLLQGLGVPNSNTVLNRSFTALLLALLL
                     EDSNNKSWISEEDQIKIMNQAFSWLIDELDFRGFDEELGWIHVFAHGADLLTEIVKMK
                     CFSKDDSSKILTIIKRVMITDDILTLNG"
     gene            958..>1398
                     /locus_tag="VBD89_002030"
     CDS             958..>1398
                     /locus_tag="VBD89_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEO7136500.1"
                     /translation="MRDEQEIYNLVLNIANQDKRIEAVLLNGSRANPNVPKDDFQDYD
                     IVFVTNFIEDIISDTNYHKKFGDILIMQKPNEFRNKTEYNCFAYLMQFQDLTRIDLRL
                     IKPEFLEDYLDDAFSKVLLDKKNKYLDYNFERSSLYETKQLSEDE"
BASE COUNT          540 a          156 c          253 g          449 t
ORIGIN      
        1 tagttctatc gtatatttat ggtatgcctc aattttttcc taatagaggt tacaaggtag
       61 gccttcgcta tatttctaga gtcaaagaat aagtactaaa aatttctgat ataaaagtga
      121 gtatccaagt aaaaaattaa agttactata atcattttaa agtgaaaacg aaaactctaa
      181 tgttacactt tggaaaatat aaaatataaa gtcatgcaag gagaaacatg gaagcaagtg
      241 atgtaatcaa aaagatagaa aataattatc caattgatga gaatgtttta tctttttgat
      301 tacatcatat aggagattgg aacccgagag ttcgagatga aatagtatat gggtcttggg
      361 ttaaattagt aatagaacat aaactgatgc aaagccagaa attaaatatc cttcagagag
      421 ttttaaggga taaatttctt ttacaaggat taggagtacc caacagtaat actgtgttaa
      481 acagatcttt tactgctcta ttactagctt tattgttgga ggactcaaat aataaaagtt
      541 ggatatctga agaagatcag ataaaaatta tgaatcaggc attctcctgg cttatagatg
      601 aacttgattt tagaggcttt gacgaagagc ttggatggat acatgtcttt gctcatggag
      661 ctgatttatt aacagaaata gtcaagatga aatgtttttc aaaagatgac agttcaaaaa
      721 tcttaactat cataaaaaga gtgatgataa ctgatgacat actaacatta aacggttgaa
      781 aatattttga atcataatca gttgttcaga aaaatttaaa attgaatcct tggaggtcgt
      841 aaaggtattt ccatgctaat ttaagtttat gattgcatgc ttagagaaaa atacaagggt
      901 aatataaagg tattagaaaa aatatggtaa tataaagtga agtaggaggt agccattatg
      961 agagatgagc aagaaatata taatttggtt ttaaacatag caaatcaaga taagagaatc
     1021 gaagctgtac tattgaatgg atctcgggct aacccaaatg taccgaaaga tgattttcaa
     1081 gattatgata ttgtttttgt tactaatttt atagaagata ttatttctga tactaattat
     1141 cataaaaaat tcggtgatat tcttataatg cagaaaccaa atgagtttag aaataagacg
     1201 gaatacaatt gctttgctta cttaatgcag tttcaagatc tgacgaggat tgatttaaga
     1261 cttattaaac cagaattctt ggaggattat ttagatgatg ctttttcaaa agtactttta
     1321 gataaaaaga ataaatatct tgattataat tttgaaagaa gttctctgta cgaaacaaag
     1381 caattgtcag aagatgaa
//
LOCUS       DATRSG010000135         1396 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRSG010000135 DATRSG010000000
VERSION     DATRSG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.137"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..668
                     /gene="recX"
                     /locus_tag="VBD89_002031"
     CDS             <1..668
                     /gene="recX"
                     /locus_tag="VBD89_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO7136501.1"
                     /translation="GMIINAEKLENIKKFAQLSYGKNLGLYYISFKQRTEKEVIKYLQ
                     EHDIDSKIIPQIIDNLKSENWINDKNYVQSFIQQNLNNGDKGPYVIKQKLLQKGIKSK
                     IIESELKAINFQDLASKISQKLYKKYQNKLPLKALKDKLMQSLTTKGFDYQIGHTVIQ
                     NLEIEKDQELEDDLIYKELDKQYQKLSKKHDQYELKQRIINVLMRKGYQYEDIKSALR
                     EYL"
     gene            752..1285
                     /locus_tag="VBD89_002032"
     CDS             752..1285
                     /locus_tag="VBD89_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO7136502.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          584 a          203 c          216 g          393 t
ORIGIN      
        1 aagggatgat aattaatgcc gagaaacttg aaaatattaa aaaatttgcc caactatcat
       61 acggtaaaaa tctaggtctt tattatattt ctttcaagca gagaaccgag aaagaagtta
      121 ttaaatatct tcaagaacat gatattgatt ctaaaattat tccacaaatt attgataatc
      181 taaaaagtga gaattggata aacgataaaa actacgtgca atctttcata cagcaaaatc
      241 taaataatgg cgataagggg ccatacgtga taaaacaaaa attattgcaa aaaggtatca
      301 aaagtaaaat aattgaatct gaacttaaag ctataaactt tcaagactta gcatccaaaa
      361 taagccaaaa actctataaa aaatatcaaa acaaacttcc actaaaagca ttaaaagata
      421 aattgatgca gagcttaact acaaaaggat ttgattatca gatagggcat accgtaattc
      481 aaaaccttga aattgaaaaa gatcaagaac ttgaagatga cctcatttat aaagaattag
      541 ataaacaata ccaaaaatta tcaaaaaaac atgaccagta tgaattaaag caacgtataa
      601 tcaatgtctt aatgcgtaaa ggataccagt acgaagatat caaaagtgct ttaagagaat
      661 atttataaga taataactct caaacgatgt attttacaga aaattatgat aaactataac
      721 agacgtataa attgtagaaa gttggtaggc tatgagatta cctaaagaag gcgactttat
      781 tacaattcaa agttacaaac atgatggtag tttacaccga acttggcgtg acaccatggt
      841 attaaaaaca accgaaaatg ccctcattgg tgttaatgat catactttag taacagaaaa
      901 tgatggtcga cgctgggtga cacgagagcc tgcaatagta tactttcata aaaaatattg
      961 gtttaacatt atcgctatga tacgtgaaac tggtgtctcc tactattgta atctagcaag
     1021 tccgtatatc ttggatcctg aagcactcaa gtacatcgac tacgaccttg atgtcaaagt
     1081 atttgcagat ggtgaaaaaa gactactgga tgtggacgaa tatgaacagc ataaagctca
     1141 gatgaactat cctaccgata ttgattatat attaaaggaa aatgtaaaaa tattggtaga
     1201 atggataaat gagaataaag gccccttttc atcatcatat atcaatatct ggtataaacg
     1261 gtaccttgaa ttgaaaaagc gctaactaac acactaaata gtgtgttttt attaatatca
     1321 aatttaatta caatactatt gcaaaaatat atacttaaaa taaaaaaagt agaaagtgat
     1381 ctttctactt ttttaa
//
LOCUS       DATRSG010000136         1389 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRSG010000136 DATRSG010000000
VERSION     DATRSG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.138"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            212..1117
                     /locus_tag="VBD89_002033"
     CDS             212..1117
                     /locus_tag="VBD89_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7136503.1"
                     /translation="MPENIESLDIQIWVNKLAKQSVKSVASGVKKSKKGHSKDFGAFV
                     HKLDDIFDFGVINFGLQSNPVKGIKIPPKPKSLKKRIRVLHDNELTIWLEYLEILENN
                     RANRRFKIICNTLLASALRINELLALTIDDLDFMTNEIIVSKTLVWKAGNKNKGIKGE
                     MICKQSPKSDSGNRRVLVPKEILNGLKDFHQEMNNYFEDHRLPTTKLIFPTIYGNYMC
                     DRNERLTLKKRLSSLNLPDYGFHIFRHTHASMMLNAGANWKELQVRMGHKSISTTMDT
                     YAELAPTKKQEAVEIFLNKISELTH"
BASE COUNT          499 a          187 c          252 g          451 t
ORIGIN      
        1 attgtctcca cttccaaagt tattagaagg cgtattaggt gtagacggta tttgtgagct
       61 tggagaaggt gcctgatgtt gaggtgttgt cggtgcttgt ggacttggtg aaggtgcctg
      121 atattgaggt gttgtcggtg cttgtggact tggtgaaggt gcctgatatt gaggtgttgt
      181 cggtacttga gagctagaat tcggagaata tatgcctgaa aatatcgagt cattagacat
      241 ccaaatatgg gttaataaat tagctaaaca gtctgttaaa tcagttgcat caggtgttaa
      301 aaagtcaaaa aaaggacatt ccaaggattt tggagctttc gtccataaac tcgatgatat
      361 tttcgatttt ggagtaataa attttggatt gcaaagcaac cctgtaaagg gaattaaaat
      421 tccgccaaaa ccaaaatcat taaaaaaaag aattagggta ttacatgaca atgaactaac
      481 tatttggtta gaatatttag aaattttaga aaataatcgg gctaatagaa gatttaaaat
      541 aatatgtaat actcttctag catcagcact tagaattaac gagctattag ctttaacaat
      601 tgatgattta gattttatga caaatgaaat tatagtcagc aaaactttag tgtggaaagc
      661 tggtaataag aacaaaggta ttaagggtga gatgatttgt aaacaaagtc caaagtcaga
      721 ctcgggaaat cgtcgagttc tagttccaaa ggaaattttg aatggattaa aagactttca
      781 tcaagaaatg aataattatt ttgaagacca taggttacca actacaaaat taatttttcc
      841 aacaatctat ggaaattata tgtgtgatag aaatgaacgt cttacattaa agaaaagatt
      901 gtcatcactt aatttacctg attatggctt tcatattttt cgtcatactc atgcttccat
      961 gatgctaaat gcaggcgcaa actggaaaga gcttcaagtt agaatggggc ataaatccat
     1021 ttctaccact atggatacgt acgcagaatt agcacctaca aaaaaacaag aagccgtaga
     1081 aatttttctg aataaaattt ctgaattaac acattaacag ctctacattt ttctctacat
     1141 tttcttttaa atttagataa aaactgttat attagggttt aaaggcaatg aaatagacaa
     1201 taatacactt ttactgaagt taaataattg attataaaaa agcctatgaa atcaatattt
     1261 ctgggctttt tatttttaaa aataaaatct gacaaaaaag tataaattta cctcttacct
     1321 tcaggtagtt agtcattctt ttaaaactca cttttttata actgaaaata ttgttcttaa
     1381 ttttaaaaa
//
LOCUS       DATRSG010000137         1358 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRSG010000137 DATRSG010000000
VERSION     DATRSG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.139"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..857
                     /locus_tag="VBD89_002034"
     CDS             <1..857
                     /locus_tag="VBD89_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7136504.1"
                     /translation="FKSQIKTIYTYKIKKKINRKNTNHKEGVFNIYISGIDTFGSIST
                     VSRSDVNIIMTVNTNTHKVLLTTTPRDAYVKIPDGGGNQYDKLTHAGLYGVETSMKTL
                     ENLYDINLDYYARINFSSFLKLIDLLGGVTVYNDQAFTSKHGNFDFPVGQVTLNSEQA
                     LGFVRERYSLQGGDNDRGRNQEKVIAAIINKLASSQSVTKLNSITSQLQTSVQTNMTI
                     DNINDLINNQLSTGQRFTVESQALTGHGSTGELPSYAMPGAQLYMMSIDQSSLSNAKS
                     KIKNTMEE"
     gene            863..>1358
                     /locus_tag="VBD89_002035"
     CDS             863..>1358
                     /locus_tag="VBD89_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO7136505.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSF"
BASE COUNT          504 a          202 c          234 g          418 t
ORIGIN      
        1 attttaaatc gcagataaaa acgatttata cttacaaaat taagaaaaaa attaatcgta
       61 aaaatactaa tcataaagaa ggggtattta atatctatat tagcggtatt gatacttttg
      121 gctctatatc aacagtatca agatctgatg taaatattat tatgacggtt aataccaata
      181 cccacaaagt attgttaacg acaacaccac gagatgccta tgtaaaaatt ccagatggtg
      241 ggggcaatca atatgataaa ttaacccatg caggtttgta tggtgttgag acatcaatga
      301 aaacacttga aaacctttac gacatcaacc ttgattatta tgctagaatt aatttttcat
      361 catttttaaa attaatagac ctcttgggag gagtgacagt ttataacgat caagctttta
      421 caagtaaaca tggtaatttt gactttcctg ttggtcaagt aacattgaat tctgagcagg
      481 ctttgggctt tgttagagaa cgttattctc tacaaggagg cgataacgat agaggtagaa
      541 atcaagaaaa agtgattgca gctattataa ataagttagc ttctagtcag tcagtaacaa
      601 aattaaatag cattacctca cagctccaaa cgtccgttca aactaatatg actattgata
      661 atattaatga tttgattaac aatcaattgt caactggaca acgcttcact gtcgagtcac
      721 aagcattaac tggtcatggt tcaacgggtg aactcccttc atatgcaatg ccaggagctc
      781 aactttatat gatgtcaatt gatcaatcta gcttatctaa tgcaaaatca aaaattaaga
      841 acacaatgga ggaataacaa gaatgattga tattcattct catatcgttt ttgatgtaga
      901 tgatgggccc aaaacgcttg aagagagttt atctttaata gaagaaagtt atcgacaagg
      961 tgttagaata attgtttcga catcacatag aagaaaaggt atgtttgaaa cacctgaaga
     1021 tattatcttc aaaaactttt ctatagttaa acacgaagcg gaaaaaagat ttgaacatct
     1081 tcaaatctta tatggtggag aattatacta tacaagtgat atgttagaaa aactgaagtt
     1141 aaagcaaatt ccaactttaa acaatacgaa atttgcttta attgaatttt ctatgcaaac
     1201 ttcttggaaa gatattcata cagctttgtc aaatgtttta atgcttggta ttacaccagt
     1261 cgttgcgcat atagagaggt ataacgcttt agagaatcaa aaagaacggg tgaaggaaat
     1321 tattaatatg gggtgttaca cacaaataaa tagtttcc
//
LOCUS       DATRSG010000138         1347 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.140, whole genome shotgun sequence.
ACCESSION   DATRSG010000138 DATRSG010000000
VERSION     DATRSG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.140"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(1..544)
                     /locus_tag="VBD89_002036"
                     /pseudo
     CDS             complement(1..544)
                     /locus_tag="VBD89_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            722..922
                     /locus_tag="VBD89_002037"
     CDS             722..922
                     /locus_tag="VBD89_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7136506.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            964..1152
                     /locus_tag="VBD89_002038"
     CDS             964..1152
                     /locus_tag="VBD89_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7136507.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          467 a          175 c          264 g          441 t
ORIGIN      
        1 agcagtctaa ttgaacctgt ttgatatttt gataattttt ttggttgatt tgtctatgct
       61 ttaaatactt gaaaaatgct tcagttacgg cattatcata aggatatcta ggtttagaaa
      121 aagaatgcat gatattgttg tcatcaatta tttttctaaa ttccctggcc ttaaattgtg
      181 acccttggtc ggaatgaaag agaagtgttc cttcaatctt tcttttatta agagctaatt
      241 ctagagtgtc acatgctaac tttgcatcca tacgatgact caatttccaa gcaatatatt
      301 ttctagaata gagatcaatt attgcgcaga gatagacata tttcttatat ccaatagaaa
      361 tataggtgaa gtcagtagac catacttgat taggtttatc aggattaaat ttctggttga
      421 gtaagttttg aggatacgtt ttttgagtct ttttaagagc tgtttttgtc ttaacggtag
      481 ccattttagg gagcgccata ttcttcatca gacggtaaat tcttccttca gagatttttg
      541 tgtcttttaa aacaggttat aacacacaaa gtgttgtcta tttttatagt atacatccat
      601 actcgcataa ccatgagata atcactaaaa caagagtcgc ctgacactag cgactcttgt
      661 ttttatttgc taaagaaaga tatagatgtt atgattaata aaaataataa ggaggtgcag
      721 tatgtcacaa gaaaaactaa aatcaaaatt agatcaagca aaaggtggtg ctaaagaagg
      781 ctttggcaaa ataaccggtg ataaagagtt agaagcaaaa ggatttatcg aaaaaacaat
      841 tgctaaaggc aaagaactag cagatgatgc taaagatgct gttgaagggg cagtagatgc
      901 tgtcaaagaa aaactgaagt aacaaataac cgctcattta acgagcggtt atttgttatc
      961 tttttgtcta tcagaacaga tatagatgag tttaaagaag cgattgataa aggctatatt
     1021 tcagggaaca cagtagcgat agtgcgtaaa aacggaaaga tatttgatta tgtgttacta
     1081 cacgaagaag tgagagaaga agaggttgtt acagttgaga gagtgcttga tgtactgagg
     1141 aagttatcat aataacggac caaaattcgc cccaaaaagt attttaaaat agaataaaat
     1201 aaaaaaatat gctttttatt atggatgtat cgtgtatttt aacctttaat atatcctatt
     1261 ttaatggtag agaagttggc aggggacata ttaaaacctt ttttgagatg ttaggaagga
     1321 ttttattctt tagactagtg ttgagaa
//
LOCUS       DATRSG010000139         1315 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRSG010000139 DATRSG010000000
VERSION     DATRSG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.141"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            17..1144
                     /locus_tag="VBD89_002039"
     CDS             17..1144
                     /locus_tag="VBD89_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO7136508.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIAPFILKYSFKD"
     gene            complement(1207..>1315)
                     /locus_tag="VBD89_002040"
     CDS             complement(1207..>1315)
                     /locus_tag="VBD89_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO7136509.1"
                     /translation="NINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
BASE COUNT          371 a          221 c          212 g          511 t
ORIGIN      
        1 atattaggag gaaaaaatgc atattatcat tcaaattaca atcattttgt tggcaagcgt
       61 tcttgcaact cttatttcaa aaagaattgg aataccggct gttgttggac aactacttgt
      121 aggtatcatc atcggacctg caatgctagg acttgttcat caaaatcagg tattacatgt
      181 cctgtcagaa attggtgtta tcttactcat gtttttagcg ggtcttgaag ctaattttga
      241 tcttttaaag aagtatctta agcccagtct actggtcgca attactggtg ttattgttcc
      301 tatggctctc ttctacttct tgacacgctt gtttgggttc caaatcaata ctgctatctt
      361 ttacgggctc gtatttgctg caactagtat ttctatcaca gttgaagttt tacaagaata
      421 caatcgtgta aaaacagata ctggtgctat tattttaggt gctgctgttg ctgatgatgt
      481 cttagctgtt ttgcttctga gtgtctttat tgcaacaaat ggaagctctt ctaatatcgg
      541 attacaaatt atcattcaat tgttattctt tgttttctta tttatttgta tgaaatacct
      601 tgtacctgct cttttcaaat taatcgaaaa ggttcatttc tttgagaaat atactattct
      661 tgctatcctt atctgttttt ctctatcaat tttagccgat aaagttggaa tgagttctat
      721 cattggttcg ttttttgcag gattagctat tggacaaact tcttttgttg ataaggtaga
      781 acataaaatt tcacttctta gttacacctt ctttatccca attttcttcg cttcaattgc
      841 actacctctt aaattcgatg gaatgatgtc tcaccttcac acgatattaa tctttactgc
      901 tttggctgtt ttatcaaaat taattccagg gtactttgtc ggtcgaggct ttaatttttc
      961 aaaattagaa tctttaacaa ttggtggtgg aatggtcagt cgtggcgaga tggctctcat
     1021 tatagttcaa gttggattag ctgctaaaat tatttcaagc actacttatt ccgagcttgt
     1081 tattgttgtt atactgtcaa ctattattgc gccatttatt cttaaatatt catttaaaga
     1141 ttagtaaaaa aacacaaaca tttatttagg gtaaaaaaag tctagcttat aagctagact
     1201 tttttattaa ctaccaaact gctggaaaaa attaacaatt tgttgccaaa tattagctaa
     1261 aaatccctta cctgattcaa cagctttatt agcattaaaa ttgacattga tattt
//
LOCUS       DATRSG010000140         1293 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRSG010000140 DATRSG010000000
VERSION     DATRSG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.142"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            100..432
                     /locus_tag="VBD89_002041"
     CDS             100..432
                     /locus_tag="VBD89_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136510.1"
                     /translation="MKNLFATTEASSRKQEQDRIVNYIKQHVELTNGNQIKKIEFIDF
                     QKNEMTGTWGISTKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNVNDI
                     EVIYMKKE"
     gene            434..943
                     /locus_tag="VBD89_002042"
     CDS             434..943
                     /locus_tag="VBD89_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136511.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRSDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAEPDTSEIVIVYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGPDIHNMI"
     gene            1114..>1293
                     /locus_tag="VBD89_002043"
     CDS             1114..>1293
                     /locus_tag="VBD89_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEO7136512.1"
                     /translation="MKKKYWILLILLLTVLSGGGYMVFKQHTEKEKMIAIAHSAEAMQ
                     IYEKHMKANDSRAFSE"
BASE COUNT          516 a          143 c          252 g          382 t
ORIGIN      
        1 tatttgaatg gtatagaaaa aacagaggtt tatttgctta tgaaaaaacg tatatggtat
       61 ttgataataa taatcacagt aattttagga ggactagcca tgaaaaactt atttgcaaca
      121 acagaagcat catcaaggaa acaggaacaa gatagaattg tcaattacat aaaacaacat
      181 gttgagttaa caaatggtaa tcaaataaaa aaaattgagt ttatcgactt tcaaaaaaat
      241 gagatgacag gtacatgggg aatttctact aaaattaatg aacaattttc gattagtttt
      301 tctgaagata gaattggtgg taaacttaga gcattaggat atcaaccgaa tgaaataggt
      361 ttttcaaagg acatcaatag taataatcaa aatgttaatg atattgaagt gatttatatg
      421 aagaaagaat agaatgattt cagaagaaag ttacagatat ttagttgagg atgcttatcg
      481 agttgattca aaaaaagtaa aaatacctct gaaaagaagt gatattgtag ggaacagtga
      541 ctatgttatt attgaaccac ctatagataa tacttcaaat ggtatgcaag ctatggtagt
      601 tgctccgata aaagaaggaa tgacagctga accagatact agtgaaatag tgattgttta
      661 tgcaggtact aatttaggtg agcgtttaga catagctaca gatgttgaaa tggttgctgg
      721 aggggataca tatttacttg ctgatcctaa aaccaagact tttaggaaat ctcagggaaa
      781 atcagcacta gagtacgcag aaaaaatatc gagtaaatat cctaattcag aaattactac
      841 aaccggtcac tccttagggg aatcagaggc actatatgtc gctttaaaaa tgggttggat
      901 gaatgttggt tataatggtc cagatattca taacatgata taaaatgacg aaatagatta
      961 tatgaaatct catccagaat aatttagaaa aaaatcgaaa aatatgagtt gtttgagtta
     1021 aaattttaac tatggagata gagtcctgta ttatttgatt ttgtagtaaa ctagttagga
     1081 atttattcat aggatattat aaaggagtta atgatgaaga aaaaatattg gatattacta
     1141 atattactat taacagtttt atcaggaggc ggatatatgg tttttaaaca acatacagaa
     1201 aaagaaaaaa tgattgcaat tgcacacagt gcagaagcga tgcaaattta tgagaagcat
     1261 atgaaagcaa atgattcgcg agctttttca gag
//
LOCUS       DATRSG010000141         1212 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRSG010000141 DATRSG010000000
VERSION     DATRSG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.143"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            358..>1212
                     /gene="ltrA"
                     /locus_tag="VBD89_002044"
     CDS             358..>1212
                     /gene="ltrA"
                     /locus_tag="VBD89_002044"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO7136513.1"
                     /translation="MSELLDKISSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKKLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     HFSEYSYGFRPNRSCETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     QDGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKGLEKRGLR
                     FVRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFW
                     KSPKGWKCR"
BASE COUNT          390 a          238 c          288 g          296 t
ORIGIN      
        1 gcgtatatag cggatgaggg ggctataaac tctaaagtcc aaataggtag tcgtaaccta
       61 tatacgtaaa tcacgagagt aaacgaggaa agatgtccga cttatcccgt gaggtcctgt
      121 caagggtaaa acctagtaac aacgaacgac aagaagtcag ccgaacccat agtagtgatg
      181 aagtttctgt aatggaaatg gaacgaaggg gtgaacaact aatcgaagta atcctacttc
      241 acttgtgtct gtaaaacaaa cggtctgata gaactagatg cggttacgta ttgactagag
      301 gaaggttcac caatgtaaga tatcatgcag acaacgaaaa aagaaaggaa tacgcacatg
      361 tcagaattgc tagataagat atcatctcgg aacaatatgc tcgaagctta caagcaagtg
      421 aaatcaaaca aaggttctgc tggtatcgat ggggtcacta tcgagcagat ggatgactat
      481 cttcaccaaa attggcgaga aaccaagaaa ctcatcaaag agaggagcta taaacctcaa
      541 ccggttctca gggttgaaat cccaaaacca aacggaggag ttcgtaacct aggtatcccg
      601 acggctatgg atagaatgat tcagcaggcc atcgttcaag ttttgagtcc actctgcgaa
      661 aaacattttt cagagtatag ctatgggttc agacccaatc gctcctgcga aacagccatt
      721 gttcagctac ttgagtattt aaacgatggc tacgagtgga ttgtggacat tgacttggaa
      781 aagttcttcg atactgttcc gcaagacaga ttgatgtccc tggttcataa tatcattcaa
      841 gatggcgata cggagtcact gattcgtaag tacttccatt cgggagttgt tattaacgga
      901 cagcgacata agactttagt cgggacacct caaggcggga atctatcacc cctcctatct
      961 aatattatgc ttaatgagtt agacaaaggg ttggaaaagc gaggtcttcg ctttgtccgt
     1021 tacgccgatg actgtgtcat cactgtcgga agcgaagcag ctgctaagcg ggtcatgcat
     1081 tcggtcagta gctatattga gaagcgatta gggttgaaag tcaacatgac taagaccaag
     1141 attgtcagac cgaacaaact caaatacctc ggatttggtt tctggaaatc tccaaaaggt
     1201 tggaagtgtc gt
//
LOCUS       DATRSG010000142         1118 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRSG010000142 DATRSG010000000
VERSION     DATRSG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.144"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(108..>1118)
                     /locus_tag="VBD89_002045"
     CDS             complement(108..>1118)
                     /locus_tag="VBD89_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEO7136514.1"
                     /translation="FANQDLADYLKELIGDSYLTDATQLEKLLTYADSNEVHDKLAAI
                     KFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYVIHKYLEIKRGHF
                     PSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNVHLVENYNVTVAE
                     KLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAELAGKENIYTFG
                     KDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAERLKRLHDELVGK
                     DWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSDRTIEQYNQDIWH
                     SN"
BASE COUNT          358 a          193 c          163 g          404 t
ORIGIN      
        1 taagttctgc tcagttctat ttttaaagtc tacggacaaa aataaatact tatagtttta
       61 ttagacaaga aaaaagtaag gttgaaaacc ttacttttag agacttttta atttgaatgc
      121 caaatatctt gattatattg ttcgattgta cgatctgatg agaagaaccc tgctttagca
      181 atattgtgta taactttctt attccatgat tcataatctt cataatcagc aagcacttgt
      241 tctttaacag caatgtattc tttcaaatca atcaatgtca tgaaccaatc tttacctact
      301 aactcatcat gcaatctctt tagtcgttct gcattaccga gtgacactat atcatcacta
      361 atgataaaat caacagcttc acgaattact ttgtctttat catagtaatc ttttgaacga
      421 taaccagatg tttcataaag attaatgata gtatcagagt ctttaccaaa tgtgtagatg
      481 ttctctttgc cagctaattc agctatttca acatttgccc catccatagt accaagagta
      541 agagcaccat tcaacataaa tttcatgtta cctgtaccag atgcttcttt ggaagccaat
      601 gaaatttgtt ccgaaatatc tgttgcaggg attaactttt ccgctactgt cacattgtaa
      661 ttttctacta aatggacatt aaggtattta ttaacttctg gatcattatt aattaattct
      721 gataagcaga gaattaagtg aatgatatcc tgtgcaatag tataggctgg agcagcttta
      781 ccaccaaaaa taactgttaa tttacgtgat gggaaatgcc cacgtttaat ttctaagtat
      841 ttatgaataa catataaagc attcatttgt tggcgcttat attcgtggaa tcgtttgatt
      901 tgtgtatcaa taattgagta ttcatccaat tcaatacctt tattttcctt aaggtaacgt
      961 ttaagagcca gtttgttttt aaatttaata gcagctaatt tatcatgaac ttcattgcta
     1021 tcagcataag ttaacaattt ttcgagctga gtcgcatcag taagatagct atccccaatt
     1081 agttctttca agtaatctgc taaatcttgg ttagcaaa
//
LOCUS       DATRSG010000143         1076 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.145, whole genome shotgun sequence.
ACCESSION   DATRSG010000143 DATRSG010000000
VERSION     DATRSG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.145"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            51..974
                     /locus_tag="VBD89_002046"
     CDS             51..974
                     /locus_tag="VBD89_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7136515.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
BASE COUNT          351 a          182 c          184 g          359 t
ORIGIN      
        1 atttagctga tctaattgaa tttatttaca ttctagttta aactagaatt atgagaattc
       61 aacaattaca atatgttatt aagattgtag aaactggtag catgaatgaa gccgctaaac
      121 aactctacat tacccaacct tcactttcaa acgctgttag gaaccttgag actgagatgg
      181 gtatccaaat ttttattcga aatccaaaag gaataacgtt aacaaaagat ggtatggaat
      241 ttctgtcata tgcgcgacaa attctagaac aaacagcact tcttgaagag cgttataagg
      301 gtgataatac aagtcgtgag ctgttcagcg tttcttcaca acactatgca tttgtggtta
      361 acgcttttgt agctctcttt aatggcacag acatgaccca atacgaactt tttttacgtg
      421 aaactaggac ttgggaaatt attgatgatg ttaaaaattt ccgttcagag attggtgttc
      481 tgtttcttaa tagctataat agagatgtat taaccaaatt attcgacgat aattccttaa
      541 ttgccacaac tctttttact actacacctc atattttcgt gagcaaatcc aatcccttag
      601 ctaatagaaa aaaattaagt atgaaagatt tagaagatta tccttatctg agttatgatc
      661 aaggcctgca taattctttc tacttttctg aggaaatgat gtctcaaatt cctcacccaa
      721 aatccattgt tgtcagtgat agagcaactc tgtttaattt aatgattgga ttagatggtt
      781 atacagtggc aactggtatc ctcaactcaa aattgaacgg tgacgaaatt gtcgcaatcc
      841 ctcttgacgt ggatgatgtt attgatattg tctatatccg acacgacaaa gcaaaccttt
      901 caaaaatggg acaaaaattt attgactatt tattagagga agtttcgttt aatcataaaa
      961 ataaacagtc ctgagattgt tgtactgacc cccaaaagtt ggacaattta ttttaagtga
     1021 aggatttagt tctgtattgc acaggactaa gtccttttag ttttgctttg attcgc
//
LOCUS       DATRSG010000144         1013 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRSG010000144 DATRSG010000000
VERSION     DATRSG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.146"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            85..990
                     /locus_tag="VBD89_002047"
     CDS             85..990
                     /locus_tag="VBD89_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7136516.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
BASE COUNT          353 a          197 c          153 g          310 t
ORIGIN      
        1 ttagctcaaa atgatagtaa acttatagcc ataacgtatc ataacttatt attaatctaa
       61 ggatatcaaa ctggttataa agtcttgaaa aatcaacaaa aattaatgta tctagagagt
      121 atcgagctat actcaaacat cactaaagcc gctgcacacc tttttatttc tcaaccttac
      181 ctaagcaaag tgatcaagca attagaaaat gagttagaaa taaaactcat ccagagccaa
      241 ggacaccaaa cctttttaac ttatgctgga cagagatatc tattctacct aaaagaaatt
      301 gatatgattg agcgtcaaat ggccaaagaa ctatatctca tccgttctga taaaaaagga
      361 gaaatcacac ttgggattaa ctctggctta gcaagttcta ttctggcaaa tgtacttccc
      421 aaatttaact tggagcaccc agaaatttct gtcaaattac tcgaaaacaa tcaaaatatt
      481 tctgaacaat tggtagcgag tggtgatatt gacctagctg ttgggatggc tcctatcctt
      541 tataaagatg gaattgcatc aaccactatt taccgcgacg aattgttttt aatgatacct
      601 accaccagcc aactctacaa cgctgaaaaa cgtggacaga ttattccttt tgaatatcct
      661 atttcagttc tagacaatga acctcttatc ctaacaccat tagagtacgg aattggcaaa
      721 acaattgcac aattctatga gcttcatcac atgtcattaa atcagatgat tacaactagt
      781 accgttccta cagcagctag cctatctttg tcagggatgg gagcaacatt tgttccacaa
      841 actctcattc atcgctattt ggataaagag tgcaatgtct accattttca taagaataag
      901 ttattttcag agtacattat gatttataaa aaagatgttg aattatctgg aattgctctc
      961 ctactttata aagctttttt aaccaagtag tttcgctaat tctattgaaa atc
//
LOCUS       DATRSG010000145         1011 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRSG010000145 DATRSG010000000
VERSION     DATRSG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.147"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..907
                     /locus_tag="VBD89_002048"
     CDS             <1..907
                     /locus_tag="VBD89_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7136517.1"
                     /translation="VNLVWKRKEYKVEIPQTESFNQLKKSILYMELSKAAQKSIAIHL
                     GIELNEGLLDYLYLVFCISDGPLFSNRDDININEEFIFASYKISKSKAFKNFCGRLLG
                     REFIDSQHFKDINAYFLRHFICYCYYFIPDFYFLNTSRLSYSKDLYHLLDKGLADIFN
                     LKGGNLTFSKHETVLLTMQLSNLIETFLAPLSVYVISSSNIRLQTYQVMLNQYFTSKI
                     AEFFFVNYQTTQIDEKLLKKADIIIAERRYISSLKNDSGVAIQDILEIGMNNKQYHEL
                     LLQAIIEKRDQKVFEYISKMKHKIL"
BASE COUNT          370 a          127 c          151 g          363 t
ORIGIN      
        1 ggtgaattta gtttggaaaa gaaaagaata taaagtagaa attcctcaaa cagaatcctt
       61 taatcaacta aaaaaatcta ttctctacat ggagttatcc aaagcagctc aaaagagtat
      121 tgctatacat cttggtatag agttgaatga aggtttactt gattatctat atttagtgtt
      181 ttgtattagt gatggtcctc tatttagcaa tcgagatgat atcaatatca acgaagaatt
      241 tatttttgct tcttataaga tatcaaagtc taaagccttt aagaattttt gtggtcgatt
      301 attgggtaga gaatttattg actcgcagca ttttaaagat attaatgcct attttttgag
      361 acattttatt tgttattgtt attattttat tcctgatttt tattttttaa atacatcaag
      421 gctgagctat tctaaggatt tatatcacct attagataaa ggtctggctg atatttttaa
      481 ccttaaagga gggaatttaa ctttttcaaa acatgagaca gtgctactaa caatgcaatt
      541 atctaactta atagagactt tcttagctcc tctttctgta tatgtaattt catctagtaa
      601 tattagatta caaacctatc aagtaatgtt aaaccaatat tttacatcta agatagcgga
      661 attctttttt gtaaactatc aaacgactca gatagatgaa aaattactaa aaaaagcaga
      721 tatcattatt gctgagagac gctatatatc ttctttaaaa aatgactcag gtgtggctat
      781 acaagatata ttagaaattg ggatgaataa taagcagtac catgaactac ttttacaagc
      841 tattatcgaa aaaagggacc aaaaagtttt tgaatatatt tcaaaaatga aacataaaat
      901 actttgatag actctatatg agctttatta aaagggataa aatcgaaatt taaaatttaa
      961 ttcgaaaaga tattgacagt aatctagaat ttcgatatac tagattttgt c
//
LOCUS       DATRSG010000146          927 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.148, whole genome shotgun sequence.
ACCESSION   DATRSG010000146 DATRSG010000000
VERSION     DATRSG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.148"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(150..761)
                     /gene="rpsD"
                     /locus_tag="VBD89_002049"
     CDS             complement(150..761)
                     /gene="rpsD"
                     /locus_tag="VBD89_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO7136518.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
BASE COUNT          300 a          164 c          173 g          290 t
ORIGIN      
        1 aaaaaattac tcattacaaa ctgacagtgt tgatttttat tctaatgtta gtttaaaatc
       61 tttttggttc attaaatata atgactcact aaaaagtata tagcaaaaaa agcctataaa
      121 ataggctttt aaagtatgta atgtaataat taaagcattt tgttgtagaa ttcaacgaca
      181 agtgcttcat tgatttctgg gttaatttca tcgcgttctg gaaggcgagt caatgaacct
      241 tcaagttttt cagcatcaaa tgatacgaaa gctggacgtc ctaaagtagc ttcaacagct
      301 tcaaggattg caggtacttt cattgatttt tcacgaactg aaataacttg tcctggagta
      361 acgcggtatg atgggatatc aacacgttta ccatcaacaa ggatatgacc atggttaaca
      421 aattgacgag cttggcgacg agttgttgca agtcctaaac gataaacaac gttatcaaga
      481 cgacgctcta aaagaaccat aaagttgaaa ccaagagttc cttcttttgc tttagtagct
      541 tgtacgaaca agttacggaa ttgtttttca ccaagtccgt atgaaaaacg aagtttttgt
      601 ttctcagcta actgcaagcc atattctgaa agcttgctac ggttattagg accgtgttga
      661 ccaggtacgt agttacgacg tgccaattct ttacctgtgc ctgtaagtga taaaccaagg
      721 cgacgtgatt gtttccatga tggaccagta taacgtgaca tctaaatgtc ctcctctaaa
      781 ataattgagg aaataacgat ttaaaaaatc ctgattcgtg caggggattt tcgcctaaac
      841 agcaaagggt actagattag ttaaccacct gttgacgagc ttcatatttt tctgctgtta
      901 tttcacacaa gctatattat atcatga
//
LOCUS       DATRSG010000147          925 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.149, whole genome shotgun sequence.
ACCESSION   DATRSG010000147 DATRSG010000000
VERSION     DATRSG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.149"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..>925
                     /locus_tag="VBD89_002050"
     CDS             <1..>925
                     /locus_tag="VBD89_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7136519.1"
                     /translation="LSIISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGG
                     NVKGEDIKKISQTNSIDSYVKRINSVADLVDHDIIETQDTLANQSPERAKNFKRTVML
                     TGVNDSAKETKFVSEAYKLVEGKHLENKDKNKILMHKDLAKKNNLKVGDKIKIKSNLF
                     DADNEKVANETVEVEIKGLFDGHNSGGVSAAQELYENTLITDVHSAAKVYGNTEDTAV
                     YQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLF
                     VGSLIFAGVVVSLLLFLWMNARKKEIAVLLSL"
BASE COUNT          385 a          116 c          163 g          261 t
ORIGIN      
        1 tttaagcatc attagtttat ctattaaaga tgccacagat agagcttcta aggaaacatt
       61 tgctaatata acaaacagtt tttctatgga aataaataga caagtaaatc caggaacacc
      121 taggggtgga ggaaatgtaa aaggtgaaga tattaaaaag atatctcaaa caaacagtat
      181 agactcctat gtaaaaagaa taaacagcgt cgcagaccta gttgatcatg atataattga
      241 aactcaagat acacttgcaa atcaatcacc tgaaagagca aagaatttta agagaacagt
      301 tatgttaaca ggggttaatg actcagcaaa agaaacaaaa tttgtatcag aagcatataa
      361 gttagtagaa ggaaagcatt tagaaaataa agataaaaat aaaattttaa tgcataaaga
      421 tttggcaaaa aaaaacaatc tcaaagttgg agataaaata aaaataaaat ctaatttgtt
      481 tgatgctgac aatgaaaaag ttgcaaatga aactgtagaa gtagaaatta aaggtttatt
      541 tgatggtcat aatagcggtg gggtaagtgc agcacaagaa ctatacgaaa atacactaat
      601 cactgacgtt cattcagctg ctaaagttta tggtaataca gaagatacag ccgtatacca
      661 agatgcaaca ttctttgtaa aaggtgataa gaatcttgat agtgtaataa aagatcttgg
      721 gaaactagat ataaattgga gagaatataa tttgattaaa agttcatcaa attatcctgc
      781 gctgcaacaa tctatatccg gtatctattc tatttcaaac aaattatttg ttggctcatt
      841 aatatttgca ggagttgtag tttcattatt acttttctta tggatgaatg ccagaaagaa
      901 agaaatagca gtattactat catta
//
LOCUS       DATRSG010000148          888 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRSG010000148 DATRSG010000000
VERSION     DATRSG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.150"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            28..216
                     /locus_tag="VBD89_002051"
     CDS             28..216
                     /locus_tag="VBD89_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEO7136520.1"
                     /translation="MITHSDIPDLQKQLETAYQVPYSFALGIYISTIESIILNWIDHD
                     FTEEPEEIARYITSVVRI"
     gene            complement(288..806)
                     /locus_tag="VBD89_002052"
     CDS             complement(288..806)
                     /locus_tag="VBD89_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO7136521.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKRIDEVDRTP
                     AENLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
BASE COUNT          292 a          158 c          104 g          334 t
ORIGIN      
        1 aacataatta aaaactatat caaagagatg attacacatt ccgatatacc agacttacaa
       61 aaacaactgg aaacagcata tcaagttcca tattctttcg ctcttggtat ttatatctca
      121 acaattgaaa gtattatttt aaattggata gatcatgact ttactgaaga gcctgaagaa
      181 atagcaagat atattacatc tgtcgtgaga atttaatatt tgtattagaa aaaactatcg
      241 tctttggtaa ccatttttat gaaatttttt aaagttaaaa atataattta taatactatc
      301 ttcttccatt ttctgatttt agttctgaaa gttccaaaag gtgcaaccgt atttacatga
      361 ataaacttat acacttccca agtcgctgtt ttagtcgctt catcagccca ctttctcata
      421 tgcgcttcaa ataattcttc ctcactcaac aaatcaatca ttgcatagat agaattaata
      481 ttttcattta attttgcttt caactcttgc agagataaat gagcgtaggt atctgtgaac
      541 cactgatata attcaccaag ttgattccat ttaaatttat ccgatggtgt ttttacttga
      601 agtccctttc tttcatcttc ttcccattta agaaccaagt tggtccagcc aacctgataa
      661 gaaaggtttt ctgctggagt tctatcaact tcatcaattc ttttatcttt tagattctct
      721 ggaatattat taaattccat aatgtatttc tcaaatgttt tacttatttc ttttttcaac
      781 tcttctttat tttcataaac tctcaaaaaa catctccttt ctgatacata ttattaataa
      841 tgatacggaa acaaccccaa agtattgtat tatagaatta gatgactg
//
LOCUS       DATRSG010000149          859 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRSG010000149 DATRSG010000000
VERSION     DATRSG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.151"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..690
                     /locus_tag="VBD89_002053"
     rRNA            <1..690
                     /locus_tag="VBD89_002053"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            765..>859
                     /gene="rrf"
                     /locus_tag="VBD89_002054"
     rRNA            765..>859
                     /gene="rrf"
                     /locus_tag="VBD89_002054"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          223 a          182 c          251 g          203 t
ORIGIN      
        1 aatccctatc ggagacagtg tctgacgggc agtttgactg gggcggtcgc ctcctaaaag
       61 gtaacggagg cgcccaaagg ttccctcaga ctggttggaa atcagtcgta gagtgtaaag
      121 gtataaggga gcttgactgc gagagctaca actcgagcag ggacgaaagt cgggcttagt
      181 gatccggtgg ttccgtatgg aagggccatc gctcaacgga taaaagctac cctggggata
      241 acaggcttat ctcccccaag agttcacatc gacggggagg tttggcacct cgatgtcggc
      301 tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc attaaagcgg
      361 cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc gcgggcgtag
      421 gaaatttgag aggatctgct cctagtacga gaggaccaga gtggacttac cgctggtgta
      481 ccagttgtct tgccaaaggc atcgctgggt agctatgtag ggaagggata agcgctgaaa
      541 gcatctaagt gcgaagccca cctcaagatg agatttccca tgattttata tcagtaagag
      601 ccctgagaga tgatcaggta gataggttag gagtgtaatt gtggtgacac atttagcgga
      661 ctaatactaa tagctcgagg acttatccaa aaagagatat tgacaacgtt acggtttctt
      721 gttagactat agatattcaa ttttgagtga ataagactca atagttaagt gataattgcc
      781 taggagatac acctgttctc atgccgaaca cagaagttaa gccctagaac gccggaagta
      841 gttgggggtt gccccctgt
//
LOCUS       DATRSG010000150          790 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.152, whole genome shotgun sequence.
ACCESSION   DATRSG010000150 DATRSG010000000
VERSION     DATRSG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.152"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            60..782
                     /locus_tag="VBD89_002055"
     CDS             60..782
                     /locus_tag="VBD89_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7136522.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNSNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT          309 a           98 c          158 g          225 t
ORIGIN      
        1 gatgagtatg gtaaaatcct ttacaatata tcattatcga aagaggttat aacatctaaa
       61 tgagacttga taaattttta gtagaatgcg gacttggttc acgtactcaa gtcaaattaa
      121 tattgaaaaa aaagcagatt agtgttaata gtaatagcga gacatctcca aaagtacagg
      181 tagatgaata tagagatgag attaaatata atggaactct agtgtcatat gaaaagtttg
      241 tttattatat gcttcataaa cccaaaggtg ttatatcagc aacggatgat ccttcgcata
      301 aaaccgtatt agatttgcta gacaaaactg ctcgagataa agctgttttt ccagtggggc
      361 gtttagatat tgatacaaca ggccttttgt taataactaa taatggggaa ttagcgcata
      421 aaatgttatc acctaaaaaa cacgttgata aatgctatga ggcaaaaatc tcagggataa
      481 tgacagaaga cgatattttg gcatttgata aagggattat tctgaaagat tttacttgtt
      541 taccagcatt attagaaatt gtggaagtaa atcaggtgaa gaagcagagt cttgttaaaa
      601 tcactattaa ggaaggaaaa ttccatcaag ttaaacgtat ggttgctgcg tgtggcaagg
      661 aagtcttaga gctaaagcgt ctaagtatgg gaaatctaca attagataaa cagctagaaa
      721 gtggacaatg gagacggcta acaataaaag aaatagaaaa attagaaaaa catatgcaat
      781 aatataaaat
//
LOCUS       DATRSG010000151          779 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRSG010000151 DATRSG010000000
VERSION     DATRSG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.153"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..129
                     /locus_tag="VBD89_002056"
     CDS             <1..129
                     /locus_tag="VBD89_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7136523.1"
                     /translation="SDTVMVVRKNGKIFDYVLPHEKVREEEVVTVERVEDVMRELE"
     gene            535..732
                     /locus_tag="VBD89_002057"
     CDS             535..732
                     /locus_tag="VBD89_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136524.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
BASE COUNT          294 a          102 c          148 g          235 t
ORIGIN      
        1 agtgatacag tgatggttgt gcgtaaaaac ggaaagatat ttgattatgt gttaccacac
       61 gaaaaagtga gagaagaaga ggttgtgaca gttgagagag tggaagatgt tatgagagaa
      121 ttggagtaat gttcgtcgtg acataagcag ctatcaatag cctgccccac aaaaaagcat
      181 attatagttg gggcgaattt ggggcataag ttaaaaacta atctatgtta acaatgatac
      241 gacttttacg tttactactc ataacgactt atatagatat gttttagacc ttataatgcc
      301 aaccgaaaaa actaactctt aaagacgctg ttaaataatt cgtctagaaa aaccttgtca
      361 tatcaatgtt tattgatagc gacaaggttc ttttttatat ccaatactca ataaacgact
      421 agagagctca aaggaattta gagtttcaaa tgtgttagag catacccttg aattatctta
      481 actgtgatgt tataattcct aaaagataag ttaaaagaga ggttgcgaaa gagaatgtca
      541 gaaaaagcta tactattaat tatccttatt gcttttgtta ttatcgctat tataaagata
      601 gctctgttaa agttatatat gaatacgata aaacctaaag tagataatga aggaaacatc
      661 ataacaaagc ctgtaaagga tgatttaaca cccgaggaac gtgaagaacg tgaatttgat
      721 aaagattggt aaaatcaata taaaagagca aacaaggttt gctcttttat tatttttaa
//
LOCUS       DATRSG010000152          743 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.154, whole genome shotgun sequence.
ACCESSION   DATRSG010000152 DATRSG010000000
VERSION     DATRSG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.154"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(37..651)
                     /locus_tag="VBD89_002058"
     CDS             complement(37..651)
                     /locus_tag="VBD89_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7136525.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNSDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
BASE COUNT          292 a          125 c          103 g          223 t
ORIGIN      
        1 aaaggctctt ttataattca ttggtaacta ctatttttat tgtaactctt tcaaagatac
       61 aatagcagac aaaccacaat atgacgatgc tcctagtaac aacgacccta aaatatccgt
      121 taaaaaatga ttttctaaat aaagtcttga aaaaataact gatgcaagga ttactaatgc
      181 aaatattacg aaaactactt gtgacaatac tcttctcaat ctctgtcttg caaccactaa
      241 aacaagaatc attaataaaa acgttgatat cgtatgtcca ctgggaaaac tataacctga
      301 gtcaaaaata agttgattca taggtctatg acgtgcgatg atatctttta aaagcaatgc
      361 caaagcattg gtaccaaaaa ataacataaa tagccacaca gctatccttt ggtttaaaaa
      421 atattgatac ccaaggataa caagtgctat taatgacgtt gtcaaagggc tgacaactgt
      481 tgatattatc gtcattaagt tagttaaatc ggagtttcgt atgctttgaa aaaaagctga
      541 agtgatatta tccaaataac ttatcatatc actttgagat ttaactgcta atgttaacac
      601 taaaaaagaa acaagtagta taacacactg gaaaaaggat ttcttgttca ctacataacc
      661 tcactttaat aaagagataa atcttcatac attatggttt aaaatattta aaaaatgctt
      721 aaagcacgcg ctaacctctt aag
//
LOCUS       DATRSG010000153          711 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRSG010000153 DATRSG010000000
VERSION     DATRSG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.155"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
BASE COUNT          258 a          100 c           96 g          257 t
ORIGIN      
        1 gctaatactc atcaatttac atccaaaaag tgtactatgt attgcaatat attcgcgaag
       61 gggttatcag gttatcaaaa gtaaaattga caataaaaaa acaccttgca attgcttgca
      121 aagtgctttg aaacgttgat ataacagtat attaacgttt tgaaaattgt gatgctttac
      181 gtgctttttt aagacctggt tggtggcacc tacatctctt taaatactaa aagtctttat
      241 taccgcactt ttgtcactat gtacttttca ctttatgtta tataatttca aataatttct
      301 tttaaataag gtaaatttct caacacttcc aaaaaaatag aatactgagc tagaggttat
      361 cttattagac aaggagcctt ttttatctca attaaatgaa aaaatataaa attgctcaaa
      421 acaaggaaaa aggtcttcaa atcaatgttt gaagaccttg aactataccg gtaccggcgg
      481 ccgagatttt aatactttag ttaaaattac tttattgatt ttaaagcatt tgggcaatct
      541 agactactta aaaagtagct tattttttta ttttttggga gaaaaattga gaaactttta
      601 taagctctga tttaatcaat ctaaatatgt accaaagatt tacaataaat ctttgataca
      661 agatattcta taaaatttca actaatttga tttaaataaa gtaattcttt t
//
LOCUS       DATRSG010000154          693 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.157, whole genome shotgun sequence.
ACCESSION   DATRSG010000154 DATRSG010000000
VERSION     DATRSG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.157"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..684)
                     /locus_tag="VBD89_002059"
     CDS             complement(<1..684)
                     /locus_tag="VBD89_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO7136526.1"
                     /translation="MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQG
                     QFNISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSP
                     DLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPHTKPDGQVDIINVSLTIY
                     NSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVT
                     NLEKILVPRI"
BASE COUNT          213 a          132 c           91 g          257 t
ORIGIN      
        1 gattcgaggt actagtattt tttctaaatt agtaacttca gttattttat tggcaatgtc
       61 tgattgcgtc ttaggattat tatcaatatc tgttttgata tcatctaaac ttatcaaaac
      121 tttatcgcga cttccctcgt cccatttagg gtcttccgct ttctttttaa cttcatctat
      181 tttatctctc aaagctgaag aattgtaaat agtcaatgaa acgtttataa tatccacttg
      241 tccatcaggc ttcgtatgag gacttccatc tgtcttcaat actgtataat taatactagc
      301 tgcaccaaca gtagtcaatt cagaaggttg tgtgaaataa ttgccgcctt gcctaatgac
      361 atactgagct tttaaatcag gtgaatttaa cgtaattaat tgtaatggat ctattttccc
      421 atttttcaca tcatataaat ctatatatgc tctaccattc tgaacatttt tagtcatatt
      481 tgtgtttaac gtaacagcac ttcctgaaat tacttcagct gcaaacacag tatctgtaga
      541 aatattaaat tgcccttgag taaaacctcc taaaaaacta ataccaatta gtacagaagc
      601 tgcaccaaac ttaaacttct taattgaaaa ccgttgtttc gtctgtaaag tatcataact
      661 gttattttta gaccttctaa acataatatt ctc
//
LOCUS       DATRSG010000155          689 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.158, whole genome shotgun sequence.
ACCESSION   DATRSG010000155 DATRSG010000000
VERSION     DATRSG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.158"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..>689
                     /locus_tag="VBD89_002060"
     CDS             <1..>689
                     /locus_tag="VBD89_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HEO7136527.1"
                     /translation="YYQPSGQFTGKVHADRFKDIDGVQIPYSSFEENYSISGTFGTNA
                     DEKSEKNGLYDRGTRQKVPQFFNKNVKYKKGEAKTTPTNDLKYVNKLNNDLIEIAITF
                     DKSYSYKEIKTMIPNNIKQNWLWIGTSTELDTSYWPTKYQFGTDPETLNTPQIFIDKI
                     KENLKGNSKVYFNNINIYSDLEKYAKKYDKVNNSDKLKFSGIILTGKAENFKQLKEKE
                     WIHASSIGANI"
BASE COUNT          309 a           91 c          105 g          184 t
ORIGIN      
        1 ctattaccag ccaagcggtc agtttactgg taaagtacat gctgatagat ttaaagatat
       61 cgatggggtt caaattccat attctagttt tgaagaaaat tatagtatct caggtacttt
      121 tggcacaaat gctgatgaaa aatcagaaaa gaatggcttg tatgatcgag gtactagaca
      181 aaaagttcca caatttttca ataaaaatgt taagtataaa aaaggagaag ccaaaacaac
      241 tccaacaaat gatttaaaat atgtaaataa attgaataat gatcttattg aaattgcaat
      301 aacttttgat aagtcctata gttacaaaga aataaaaaca atgataccaa ataatataaa
      361 acaaaattgg ttatggatag gtacaagcac agaattagat acatcatatt ggccaacaaa
      421 gtatcaattc ggtacagatc ctgaaacttt gaatacacca caaatattca tagataaaat
      481 aaaagaaaac ttaaaaggta actcaaaagt atactttaat aatataaata tctactcaga
      541 tttagaaaag tatgccaaaa aatacgacaa ggtaaataat agcgacaaat taaaattttc
      601 gggaataata ttaacaggta aagccgaaaa ctttaaacaa cttaaagaaa aagaatggat
      661 acatgcttca agtatagggg caaacatag
//
LOCUS       DATRSG010000156          680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.159, whole genome shotgun sequence.
ACCESSION   DATRSG010000156 DATRSG010000000
VERSION     DATRSG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.159"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(78..151)
                     /locus_tag="VBD89_002061"
     tRNA            complement(78..151)
                     /locus_tag="VBD89_002061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Arg,seq:acg)
     gene            complement(163..247)
                     /locus_tag="VBD89_002062"
     tRNA            complement(163..247)
                     /locus_tag="VBD89_002062"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Leu,seq:taa)
     gene            complement(256..327)
                     /locus_tag="VBD89_002063"
     tRNA            complement(256..327)
                     /locus_tag="VBD89_002063"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Gly,seq:gcc)
     gene            complement(361..433)
                     /locus_tag="VBD89_002064"
     tRNA            complement(361..433)
                     /locus_tag="VBD89_002064"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Thr,seq:tgt)
     gene            complement(444..525)
                     /locus_tag="VBD89_002065"
     tRNA            complement(444..525)
                     /locus_tag="VBD89_002065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Leu,seq:tag)
     gene            complement(535..607)
                     /locus_tag="VBD89_002066"
     tRNA            complement(535..607)
                     /locus_tag="VBD89_002066"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Lys,seq:ttt)
     gene            complement(610..680)
                     /locus_tag="VBD89_002067"
     tRNA            complement(610..680)
                     /locus_tag="VBD89_002067"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Asp,seq:gtc)
BASE COUNT          183 a          199 c          150 g          148 t
ORIGIN      
        1 gaagacagga ttcgaacctg cgacaccttg gtcccaaacc aagtactcta ccaagctgag
       61 ctacttcccg aaaaatatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
      121 caggtactct atccagttga gctaagggtg ctaaatatta tatgccgagg accggaatcg
      181 aaccggtacg atgtttacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      241 ccccggcctc taacaagcga acgacggggt tcgaacccgc gaccctcacc ttggcaaggt
      301 gatgttctac cactgaacta cgttcgcact aaagacacta tttatcctat aaaattgtaa
      361 tgccggctac atgacttgaa cacgcgaccc tctgattaca aatcagatgc tctaccaact
      421 gagctaagcc ggcaatctac taatgcgggt gaagggactt gaacccccac gccgttaagc
      481 gccagatcct aaatctggtg cgtctgccaa ttccgccaca cccgcatttc taaatgaccc
      541 gtactgggct cgaaccagtg acccattgat taaaagtcaa ttgctctacc aactgagcta
      601 acgagtctac ggtcccgacg ggaatcgaac ccgcgatctt cgccgtgaca gggcgacgtg
      661 ataaccgcta cactacggga
//
LOCUS       DATRSG010000157          660 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRSG010000157 DATRSG010000000
VERSION     DATRSG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.160"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            75..>660
                     /locus_tag="VBD89_002068"
     CDS             75..>660
                     /locus_tag="VBD89_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7136528.1"
                     /translation="MKEKVTVIIPIYNSEAYLKECVQSVLQQTHPLIEVILIDDGSTD
                     NSGEICDNLSQEDNRILVFHKKNGGVSSARNLGLDKSTGEFITFVDSDDFVAPNMIEI
                     MLKNLITENADIAEVDFDISNERDYRKKKRRNFYKVFKNNNSLKEFLSGNRVENIVCT
                     KLYKKSIIGNLRFDENLKIGEDLLFNCKLLCQEHR"
BASE COUNT          256 a           73 c          126 g          205 t
ORIGIN      
        1 gaggaaatag atcctaatca ttggagtatt gtattattat tctcaacttt tggtatagtg
       61 ggaagggcta aaaaatgaaa gaaaaagtaa cagtcattat acctatatac aactcagaag
      121 cataccttaa agaatgtgtg caatccgtac tacaacagac tcatccattg atagaagtta
      181 tactaattga tgatggatcc actgataata gtggagaaat ttgtgataat ttatctcagg
      241 aagataatcg catacttgta tttcataaaa aaaatggagg ggtctcttcg gcaaggaacc
      301 taggtctaga taaatccaca ggagaattca taacatttgt ggatagtgat gattttgtag
      361 caccgaatat gattgaaata atgttaaaaa atttaatcac tgagaatgct gatatagcag
      421 aagtagattt tgatatttcg aatgagagag attatagaaa gaagaaaaga cgaaactttt
      481 ataaagtttt taagaataat aactctttga aagaattttt atcaggtaat agagtggaaa
      541 atattgtttg tacaaaatta tataaaaaaa gtataattgg taacttgagg tttgatgaga
      601 acttaaaaat tggtgaggat ttacttttta attgcaaact cttatgtcaa gagcaccgta
//
LOCUS       DATRSG010000158          620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.161, whole genome shotgun sequence.
ACCESSION   DATRSG010000158 DATRSG010000000
VERSION     DATRSG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.161"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            34..620
                     /locus_tag="VBD89_002069"
                     /pseudo
     CDS             34..620
                     /locus_tag="VBD89_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_088186184.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
BASE COUNT          218 a           95 c          140 g          167 t
ORIGIN      
        1 gacaaaaaat ctaacgattg gggtgctttt ctgatgaaat taagttatga agataaacta
       61 gaaatatacg agttaagaaa gatcgggatg tcctggtctc agattagtca acgatatgat
      121 gttcgtatct cgaatcttaa atacatgata aaactcatgg atcggtatgg tgtagaaatc
      181 gtcgaaaaag gtagaaatga gtattatcca cctgaattaa agcaggaaat gatagataaa
      241 gtcttgattc atggttgttc tcaactctct gtttctcttg attatgctct ctctaattgt
      301 tctatactca caaattggct ttcccaattt aagaaaaatg gttatactat tgttgagaaa
      361 acaagaggga gaccgagtaa aatgggacgt aagcgtaaga aaacttggga agaaatgaca
      421 gagctcgagc gacttcagga agaaaatgag cgcttgagaa cggagaatgc ctttctaaaa
      481 aagttgaggg atttgcgctt gagggacgaa gccttacaga gcgaacggca gaaacaatta
      541 gagaaatggt cacaggagga ttccgactag acctcttact tgagataact aagatagctc
      601 gtgcaactta ctattatcaa
//
LOCUS       DATRSG010000159          555 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRSG010000159 DATRSG010000000
VERSION     DATRSG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.165"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..>555
                     /locus_tag="VBD89_002070"
     CDS             <1..>555
                     /locus_tag="VBD89_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO7136529.1"
                     /translation="KTFYLAKQAQRNYEKLLVESREGIPLNKETFWKIDRVLSNGVKK
                     GQRIYHILKTNDLEVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIK
                     EGRRYEDFIEHMNQSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSKTAIE
                     TAKHIQVIKRTLYDNKRDFFELFPV"
BASE COUNT          187 a          124 c          107 g          137 t
ORIGIN      
        1 aagaccttct atcttgctaa gcaagctcaa agaaactatg aaaaactttt agttgaatct
       61 agagaaggaa tccctttgaa caaagagacc ttctggaaaa tagatagagt gctttctaat
      121 ggggtcaaga agggccaacg tatctatcat atcctcaaaa ccaacgatct agaagtgagt
      181 tcttcaaccg tttatcgaca catcaagaaa ggctacctat ccatcacacc aatcgaccta
      241 cccagagctg tgaagttcaa aaaaaggcga aagagcacac tccctcctat tccaaaagcg
      301 attaaagaag ggcgacggta cgaggatttt atagaacaca tgaaccaatc agagctgaac
      361 tcctggcttg aaatggacac ggttattgga cggattggtg gaaaggtcct tctcaccttc
      421 aatgtcgcct tctgtaactt catctttgcc aaactgatgg attctaagac agctatcgaa
      481 actgctaaac acatacaggt cattaagagg acactctatg ataacaagag agacttcttc
      541 gaactctttc ctgtt
//
LOCUS       DATRSG010000160          555 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.166, whole genome shotgun sequence.
ACCESSION   DATRSG010000160 DATRSG010000000
VERSION     DATRSG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.166"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            216..>555
                     /locus_tag="VBD89_002071"
     CDS             216..>555
                     /locus_tag="VBD89_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136530.1"
                     /translation="MRKYIKWLIPISIFGMILGGCQMNSEPKSQSNEVKNSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDYVKLHNNQIESVQFDWSSVKVEQSGNGTPQGGDYNL
                     SLRGKFNHLQN"
BASE COUNT          245 a           57 c           87 g          166 t
ORIGIN      
        1 gtagaagaat ttatataata ataaatatta attattaatt taattagggc atttaaaata
       61 tacaaatgat tttctgttta catgttaaga ctaaaactat aaaaatacgt gaaaattttg
      121 ttatactaaa attaatttta taatgttggt gaataatagt taatttcatt atatacttga
      181 tatataaaat ttatattcta agaaaagaga acatgatgag aaaatatata aaatggttaa
      241 ttcccatatc aatttttggg atgatacttg gaggctgtca aatgaatagt gaacctaaaa
      301 gtcagtcaaa cgaagtaaaa aatagcaagc aatcagaagt gaagaaagat aaaaaaatga
      361 caaaaaaaga acaattagcc tatctcaaag agcatgagca agaaatcata gattatgtaa
      421 aattacataa caaccaaatt gagtccgttc aattcgattg gtcaagtgta aaagtagaac
      481 aaagcgggaa tggaactcca caagggggtg attataatct ttcactgaga ggaaagttta
      541 atcatctaca aaatt
//
LOCUS       DATRSG010000161          543 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.167, whole genome shotgun sequence.
ACCESSION   DATRSG010000161 DATRSG010000000
VERSION     DATRSG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.167"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..>543
                     /locus_tag="VBD89_002072"
     CDS             <1..>543
                     /locus_tag="VBD89_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7136531.1"
                     /translation="ILTVEDDKLIREGISEYLSEFGYTVIQAKDGREALSKFNSDINL
                     VILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFSLP
                     VLKARIDSLIKKNFGHLEKFEYKNLSVNFNSYTAKINDEKIDVNAKELEILKCLLDND
                     GQVLTRMQIIDYVWKDSEEI"
BASE COUNT          217 a           47 c          102 g          177 t
ORIGIN      
        1 aaatattaac tgttgaagat gataagttga taagagaggg aataagtgaa tacctttcag
       61 aatttggata tactgttatt caggctaaag atggaaggga agccttgtca aaatttaata
      121 gtgatataaa cttggttatc ttagacattc agataccttt tataaatggt ttagaagtgt
      181 tgaaagaaat tagaaagaaa agcaatttac caattctaat tttgactgca tttagtgatg
      241 aagaatataa aattgatgca tttactaatt tagtagatgg atatgtagaa aaaccatttt
      301 cattgcctgt tttaaaggct agaatagatt ctttaattaa gaagaatttt ggacatttag
      361 agaagtttga atataagaat ctttcggtaa attttaatag ttatacagct aaaataaatg
      421 atgaaaaaat agatgtaaat gcaaaagaac tcgaaatact aaaatgttta ttggataatg
      481 atggacaagt tttaacaaga atgcaaatta ttgattatgt ctggaaggat agcgaagaga
      541 tcc
//
LOCUS       DATRSG010000162          515 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.169, whole genome shotgun sequence.
ACCESSION   DATRSG010000162 DATRSG010000000
VERSION     DATRSG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.169"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..171)
                     /locus_tag="VBD89_002073"
     CDS             complement(<1..171)
                     /locus_tag="VBD89_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEO7136532.1"
                     /translation="MIKFKLFFKSTEKEEEWIDKVQKSGYTFTGINSFLPAFYYFKRD
                     KEKDKMSIKMDFK"
     gene            complement(158..493)
                     /locus_tag="VBD89_002074"
     CDS             complement(158..493)
                     /locus_tag="VBD89_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7136533.1"
                     /translation="MKRNKHLPLTETTYYILLALFEEAHGYAIMKKVEEMSGGDVRIA
                     AGTMYGAIENLLKQKWIKSISSDDRRRKVYIITETGKEIVELETNRLRKLLNTANQLG
                     FGGDGYDKV"
BASE COUNT          149 a          103 c           55 g          208 t
ORIGIN      
        1 tttaaaatcc atttttattg acatcttgtc tttttcttta tctctcttaa aataatagaa
       61 ggctggaaga aatgagttaa tgcctgtaaa agtatagcca cttttttgaa ccttatcgat
      121 ccattcttcc tctttttctg tggatttaaa aaataattta aactttatca taaccatctc
      181 ctccaaaacc caactgatta gcagtattaa gtaactttct taatcgattc gtttcaagtt
      241 ctactatttc ttttcctgtc tcagtaataa tataaacttt tcttcttcta tcgtcacttg
      301 agatagactt tatccatttt tgtttaagta aattttcaat ggcaccgtac attgtccctg
      361 cggctattct aacatcaccg ccactcattt cttcaacttt tttcataata gcatagccat
      421 gcgcttcctc aaacaaagct aataaaatat aataggtagt ttctgttaac ggtaaatgtt
      481 tattcctttt catcaatttt ctttctatac agtta
//
LOCUS       DATRSG010000163          489 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.172, whole genome shotgun sequence.
ACCESSION   DATRSG010000163 DATRSG010000000
VERSION     DATRSG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.172"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..201
                     /locus_tag="VBD89_002075"
     CDS             <1..201
                     /locus_tag="VBD89_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7136534.1"
                     /translation="DINSPIKGFPKTVITCFARETFNRILEELPHREIARTSVANLEI
                     PIYELEFKGQKIVLIKRLVMKI"
     gene            198..>489
                     /locus_tag="VBD89_002076"
     CDS             198..>489
                     /locus_tag="VBD89_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7136535.1"
                     /translation="MICLHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLG
                     NQLVEYEIKFSEANYPNKPIYAQAQAYLQDFYQSFGFQPISDIYLEDNIPHLD"
BASE COUNT          162 a           69 c           96 g          162 t
ORIGIN      
        1 gatattaatt caccaataaa aggttttcca aagactgtta ttacctgttt tgcgcgggag
       61 actttcaatc gcattttaga agaattgcct catcgtgaaa ttgctagaac tagtgtagca
      121 aacttagaaa ttcccattta tgaattagaa tttaaaggac aaaagattgt ccttatcaag
      181 aggttggtga tgaagatttg atttgtttgc atgggatgaa ttgggtagat ggtcaattgg
      241 cagcttatta tagattgata ccagaagatg ataaggtgca tttgggacgt gtgattgtta
      301 atccagactt tagaaaaaag ggattaggaa atcaattagt ggaatatgag attaaattta
      361 gcgaggcaaa ttaccctaat aaacctattt atgctcaggc ccaagcttat ttacaagatt
      421 tttatcaatc tttcggattt caacctatct cagatatcta tctagaagat aatattcctc
      481 atcttgata
//
LOCUS       DATRSG010000164          476 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.173, whole genome shotgun sequence.
ACCESSION   DATRSG010000164 DATRSG010000000
VERSION     DATRSG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.173"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
BASE COUNT          180 a           69 c           70 g          157 t
ORIGIN      
        1 tttttgataa ccttttgata acccctccgc gaacatattg taatacatag tgcacttttg
       61 ggatataaat tgttgatttt tagctagttt ataccatata acaccatata ttacagttgt
      121 cagagaaaac caggtttaaa aaaagcccgt aaagcatcac aatcctcaaa acgttaatat
      181 atacctatat atcagcgatt ggaagcactt catggttcac accatggggt gttttttgtg
      241 taatattcaa aaattgtcct aaagtcattt ttaaaacttc taaatacttg agaaaccatt
      301 gtaaatttaa ataactactt taaatagcac aaaggcaatg acaaagttag atgagatgat
      361 tctattttaa tttataattg aaaaaaagag tatatgatgg aggaattttg tatggtaagt
      421 actcaatata tgaaagtaac cttacgagaa aaattcccag ataaattaac atatgt
//
LOCUS       DATRSG010000165          475 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.174, whole genome shotgun sequence.
ACCESSION   DATRSG010000165 DATRSG010000000
VERSION     DATRSG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.174"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBD89_002077"
     CDS             complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBD89_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO7136536.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
BASE COUNT          166 a           81 c           77 g          151 t
ORIGIN      
        1 taatccgagt cttttattat ttgttagcga gtaagtcgcg gatttcagca agtaattgct
       61 cttgtgttgg ttcaacaact tcaataattt cttcttcagc aggtttctta gccataacct
      121 tgttagcagc tttaacgatg aagaataaag tagtaccaac gattaagaaa ttaataactg
      181 cacttaagaa attaccgtaa gcaacaccat tccatgaaag ctgtgcgata tttgaaacgc
      241 cagcagcttt taatacagga ttaaggataa gcggtgtaat aacgtcttta acaagtgaag
      301 tgatgatagc attaaaagct gcacctaaaa taacagcaac agcaaggtct aaaacattac
      361 ccttaaataa aaattctttt agttctttaa tcatgtgaac aagctcctta atattattta
      421 attcacgttc attatagcag atttcttttg agaaatccaa gtaataggtt tcaaa
//
LOCUS       DATRSG010000166          469 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.175, whole genome shotgun sequence.
ACCESSION   DATRSG010000166 DATRSG010000000
VERSION     DATRSG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.175"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..391)
                     /locus_tag="VBD89_002078"
     CDS             complement(<1..391)
                     /locus_tag="VBD89_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEO7136537.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPS"
BASE COUNT          162 a           68 c           86 g          153 t
ORIGIN      
        1 tactaggttt gatacattga ataaacttaa aagagctagt ctgcaacggt tgtaaataac
       61 gaaggctaac ttggtctaca atagaatatc ctattaaatc taagtgaaat ttaatgctat
      121 ctgtgatatg ctctttattt tcaatcggat gaatagaatg cataccattt ggacggatca
      181 agataatatc accagattgg ctattaaaaa aatcataatc gatgtgataa cgtgcggtcc
      241 cctcgtggac atatgaaatt tcaagttccg gatgccagtg aaataaaatg tcagggtaac
      301 catttctaac ctcagtatgc atgtgttcat agggtaataa atgccggtta tcttctaaat
      361 tatgtgatag taaattatca agtgagttca tcgtttttct ttctaaaatc gtaggatagt
      421 attattataa cagaaaacta tcaaaaaatg ggtttcgtag gatagtatt
//
LOCUS       DATRSG010000167          468 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.176, whole genome shotgun sequence.
ACCESSION   DATRSG010000167 DATRSG010000000
VERSION     DATRSG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.176"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..236)
                     /locus_tag="VBD89_002079"
     CDS             complement(<1..236)
                     /locus_tag="VBD89_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO7136538.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKV"
BASE COUNT          154 a           69 c           67 g          178 t
ORIGIN      
        1 actttcaata attgttgatt tatatcttct aataattgtg caaattgtgt gatttttgga
       61 gataattcat tgaattgggc ttcaaagcta tcaaatgctg taccatccca attttcatca
      121 ataacagctt gttcttgagt caaaactgtt aacacatctg taattgattg tgatcctgtt
      181 gtatattttt gtgctgaaat acggagttct tcaggtgtta gtttaatttg tgacatcatt
      241 tgctccctat ttttttaact gcagtatagc atatttagat gttccttaca cagataaaac
      301 tcataatagg ttaatctatt aaaaatacaa acagcgattt atattacatc tactttactt
      361 tcaaaacgga aaggttatca aaagatttta aaaaaacacc ctacaatatc ttgcaaagtg
      421 ctttgaaacg ttgatataac agtatattaa cattttgaga attgtgat
//
LOCUS       DATRSG010000168          442 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.177, whole genome shotgun sequence.
ACCESSION   DATRSG010000168 DATRSG010000000
VERSION     DATRSG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.177"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
BASE COUNT          137 a           96 c           45 g          164 t
ORIGIN      
        1 gagatttaat ttcccttttt gtgtattatt tctattctac aatagtctta cccttagaaa
       61 tatttttcca tttttcaatg atacgagcta ctctttctta ctcctcgtca tggtacctcc
      121 caaaattact tcatcaatat cttctcattg aaataatata cttgttctaa attgacctct
      181 ctggaaaaat cctgccagat aatcaaaata taatcttgat caataatatt tgctcatgaa
      241 acggctccac aaaatttctt acatctttaa cattttcata gagtgctttt actccacaat
      301 ggccaaagta cctataacaa ctgctactac tggtacaaat tggagttgtt atgattacta
      361 caaaaacact ctcagcaagc gtagagacta ttctacccac ttcattttct ctagatattt
      421 attgctccta ccttaaatta at
//
LOCUS       DATRSG010000169          425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.178, whole genome shotgun sequence.
ACCESSION   DATRSG010000169 DATRSG010000000
VERSION     DATRSG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.178"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            <1..380
                     /locus_tag="VBD89_002080"
     CDS             <1..380
                     /locus_tag="VBD89_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO7136539.1"
                     /translation="LTMRKWSIDLITRIERLNWVIRGWINYFSLGNMKSIMTQIDERL
                     RTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQKSVLKRAISKP
                     ALAKRGLVSCLDYYLERHALKVS"
BASE COUNT          129 a           79 c          116 g          101 t
ORIGIN      
        1 aactgacgat gaggaaatgg agcattgacc tgataactcg cattgaacga ttgaactggg
       61 tcattcgagg atggataaac tatttctcgc ttggcaatat gaagagtatc atgacacaaa
      121 tagatgagcg tctgcgaacc cgtattcgag tgattatctg gaagcaatgg aagaagaaag
      181 caaagcgcct atggggactc ttaaaactag gagttgctag atggatagcc gataaagttt
      241 ctggatgggg tgaccactat cagttggtag ctcagaagtc ggtactcaaa cgtgctatat
      301 caaaaccagc cctcgcaaag cgaggactgg tcagttgctt agattactat cttgaacgac
      361 atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg gtgtgagagg
      421 ggcta
//
LOCUS       DATRSG010000170          375 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.180, whole genome shotgun sequence.
ACCESSION   DATRSG010000170 DATRSG010000000
VERSION     DATRSG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.180"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            10..>375
                     /locus_tag="VBD89_002081"
     CDS             10..>375
                     /locus_tag="VBD89_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEO7136540.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
BASE COUNT          142 a           45 c           69 g          119 t
ORIGIN      
        1 caaggtaaaa tgataaattt ttctataaag gaattaaacg catttgcagt tattgggcag
       61 gaagtagaac tgactaatta tcaaaaaaag aatattcaga ttagtacaca gttttggaga
      121 aagtttaaca gtagtttgaa aaaagcgtat ctttcacaat caggaaattg ggtaaaatat
      181 gcttttatgg aaagaagaaa tggaaaactt tattatttct gttctattcc ccaaagaacc
      241 attaccccag agggctttct gtataaagaa ataccgtctt ataaatattt ggttgtggag
      301 catattggtg ctatggataa aatatatgaa acttatggca agatttatga agaaattatt
      361 cctagtacat cgtat
//
LOCUS       DATRSG010000171          363 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.denovo.181, whole genome shotgun sequence.
ACCESSION   DATRSG010000171 DATRSG010000000
VERSION     DATRSG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.denovo.181"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..>363)
                     /locus_tag="VBD89_002082"
     CDS             complement(<1..>363)
                     /locus_tag="VBD89_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO7136541.1"
                     /translation="MIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAHGLFVIL
                     TFPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHEVLFPGD
                     STWQFNPLTDPVIWILPE"
BASE COUNT          154 a           63 c           58 g           88 t
ORIGIN      
        1 tttcaggaag tatccaaatt actggatccg ttagaggatt aaattgccaa gtagaatctc
       61 ctggaaaaag cacctcatga aatagagtga aaaactgatc aaaacccaac agaaaagcac
      121 atactacaat aaaaataggc aacataatag ctatcttgaa gaagccttca tatagaaaga
      181 tacttttttg cttccatcca cgtcttaaaa aataaataac tggaaaagtt agtataacaa
      241 aaagcccatg cgctagagta aaaagatatt ttacatccgc aaaatgcttt aaaccatcct
      301 ctgaagaagg aaagctaggc atatttaaat cagagataaa aggatgagtc agatatatca
      361 tca
//
LOCUS       DATRSG010000172         6462 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.guided.080, whole genome shotgun sequence.
ACCESSION   DATRSG010000172 DATRSG010000000
VERSION     DATRSG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.guided.080"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            163..279
                     /locus_tag="VBD89_002083"
     CDS             163..279
                     /locus_tag="VBD89_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7136542.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            295..2214
                     /gene="tet(M)"
                     /locus_tag="VBD89_002084"
     CDS             295..2214
                     /gene="tet(M)"
                     /locus_tag="VBD89_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7136543.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTSFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2333..2500
                     /locus_tag="VBD89_002085"
     CDS             2333..2500
                     /locus_tag="VBD89_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7136544.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2560..2913)
                     /locus_tag="VBD89_002086"
     CDS             complement(2560..2913)
                     /locus_tag="VBD89_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7136545.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3418..3840
                     /locus_tag="VBD89_002087"
     CDS             3418..3840
                     /locus_tag="VBD89_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7136546.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3837..4067
                     /locus_tag="VBD89_002088"
     CDS             3837..4067
                     /locus_tag="VBD89_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7136547.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            4528..4731
                     /locus_tag="VBD89_002089"
     CDS             4528..4731
                     /locus_tag="VBD89_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7136548.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            4813..6030
                     /locus_tag="VBD89_002090"
     CDS             4813..6030
                     /locus_tag="VBD89_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7136549.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         2204 a         1049 c         1269 g         1940 t
ORIGIN      
        1 tcttacgtgc tattatttaa gtatctattt aaaaggagtt aataaatatg cggcaaggta
       61 ttcttaaata aactgtcaat ttgatagtgg gaacaaataa ttggatgtcc ttttttagga
      121 gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatccg
      181 gagaatatct gtatgctttg tatgcctatg gttatgcata aaaatcccag tgataagagt
      241 atttatcact gggattttta tgcccttttg ggcttttgaa tggaggaaaa tcacatgaaa
      301 attattaata ttggagtttt agctcatgtt gatgcgggaa aaactacctt aacagaaagc
      361 ttattatata acagtggagc gattacagaa ttaggaagcg tggacagagg tacaacgaaa
      421 acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacggc gataacctct
      481 tttcagtgga aaaatactaa ggtgaacatc atagacacgc caggacatat ggatttttta
      541 gcagaagtat atcgttcatt atcagtatta gatggggcaa ttctactgat ttctgcaaaa
      601 gatggcgtac aagcacaaac tcgtatattg tttcatgcac ttaggaaaat aggtattccc
      661 acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag
      721 gatattaaag agaaactttc tgcggaaatt gtaatcaaac agaaggtaga actgcatcct
      781 aatatgcgtg taatgaactt taccgaatct gaacaatggg atatggtaat agaaggaaat
      841 gattaccttt tggagaaata tacgtctggg aaattattgg aagcattaga actcgaacaa
      901 gaggaaagca taagatttca taattgttcc ctgttccctg tttatcacgg aagtgcaaaa
      961 aacaatatag ggattgataa ccttatagaa gtgattacga ataaatttta ttcatcaaca
     1021 catcgaggtc agtctgaact ttgcggaaaa gttttcaaaa ttgagtattc ggaaaaaaga
     1081 cagcgtcttg catatatacg tctttatagt ggcgtactgc atttgcgaga ttcggttaga
     1141 atatcggaaa aggaaaaaat aaaaattaca gaaatgtata cttcaataaa tggtgaatta
     1201 tgtaaaatcg ataaggctta ttccggggaa attgttattt tgcagaatga gtttttgaag
     1261 ttaaatagtg ttcttggaga tacaaagcta ttgccacaga gagagagaat tgaaaatccc
     1321 ctccctctgc tgcaaacgac tgttgaaccg agcaaacctc aacaaaggga aatgttactt
     1381 gatgcacttt tagaaatctc cgacagtgac ccgcttctgc gatattatgt ggattctgcg
     1441 acacatgaaa tcatactttc tttcttaggg aaagtacaaa tggaagtgac ttgtgctctg
     1501 ctgcaagaaa agtatcatgt ggagatagaa ataaaagagc ctacagtcat ttatatggaa
     1561 agaccgttaa aaaaagcaga gtataccatt cacatcgaag ttccaccgaa tcctttctgg
     1621 gcttccattg gtctatctgt agcacagctt ccattaggga gcggagtaca gtatgagagc
     1681 tcggtttctc ttggatactt aaatcaatcg tttcaaaatg cagttatgga ggggatacgc
     1741 tatggctgtg aacaaggatt gtatggttgg aatgtgacgg actgtaaaat ctgttttaag
     1801 tatggcttat actatagccc tgttagtacc ccagcagatt ttcggatgct tgctcctatt
     1861 gtattggaac aagtcttaaa aaaagctgga acagaattgt tagagccata tcttagtttt
     1921 aaaatttatg cgccacagga atatctttca cgagcataca acgatgctcc taaatattgt
     1981 gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg agaaatccct
     2041 gctcggtgta ttcaagaata tcgtagtgat ttaacttcct ttacaaatgg acgtagtgtt
     2101 tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg ccagccccgt
     2161 cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac ttagtgtatt
     2221 ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt ttaataaaga
     2281 tttgaattaa agtgtaaagg aggagatagt tattataaac tacaagtgga tattgtgtcc
     2341 tgtatgtgga aataaaacac gattaaagat aagggaagat actgaattaa aaaaattccc
     2401 cctctattgt ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac
     2461 tgtgattaca gagccagacg caaagacgca gagccgataa aatgagatta atacaatctc
     2521 attttatcgg ctctttccgt tatgtatgga ttcttttaat tagtcttcga tgtttcttgc
     2581 ttcgttgata ccgctggcta aagattccat taaggatagt tctttgtctg taaagctatc
     2641 catgtatttc tctatctgta atcgtcgggt gctttttacc aagttattag caggtaagaa
     2701 aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt gtatgctggg
     2761 gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc
     2821 cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc
     2881 tctaaaatca tatttatctt cttttttacg catagtagac cacctctata cattttattg
     2941 ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata ggtttatatt
     3001 taataaaaag cactactaaa cgccaataaa aaaaaccgtt atatggtagt gctatttacg
     3061 ctgttaaaat attgtatatt acttccaaat ggcggtttgt tggaggtcaa cgtcgccatg
     3121 aagtacatca tatacaataa atttccttac attgggttct tgtcaaaaaa agtcgtctat
     3181 ctgcaataga taagtacgtc caccaatgtg gttttataaa tcatatagat agaataacag
     3241 aagcatgtaa acagagaaat aaatctgttt atatgctttt ttggctattc agaacttttt
     3301 tacaaagttt atttatcagt aatgcaacaa atcccccttt cacattggga ctaagagtga
     3361 aaggagataa acgagcaagg ctcacttcct ttcctagaca gaaagggggt gagaaacatg
     3421 aaaccatctt cttttcagac cacaatagaa aatcagtttg actatatctg taaacgtgct
     3481 atggaagacg agcgaaagaa ttatatgctt tatctttcaa ggattgcaaa gcgtgaggtg
     3541 tccttttcgg atgttggcga ttatcttgtt agccagtttg cgacaacaga taactattca
     3601 actgactttc agatttttac actcaatggg ttatcagtag gcgttgaaaa tgatttgttg
     3661 agtgaagcat tacgtgagtt gccagacaag aaacgtgaaa ttctactgct gttttacttt
     3721 atggacatga gcgattcaga aattgcagac ctgttgaaat tgaaccgttc tactgtctat
     3781 cggcatagaa ccagtggact agccttaatt aaaaagttta tggaggaatt tgaagaatga
     3841 aaacacaata tcctatgatt ccctttcctc tcattgtaaa ggcaacagat ggcgataccg
     3901 aagcgattaa ccagattcta catcattaca gagggtacat aacgaagcgt tccctacgac
     3961 ttatgaaaga tgaatatggc aatcaaagta tggtcgttga tgaagtctta cgtggaagaa
     4021 tggaaaccag actgattaca aagattttgt catttgaaat taagtaatat cctctctcct
     4081 ttcgtggaag cgtgctaaac cattccacgc ttcccgaaca gggaggtttg ttattccacc
     4141 aaagcatatt gagctttcaa tgtgttttga taggctaacg agccattgtt ctttgaaaac
     4201 tgaataaaag taatcgaata cgtttcgata agaaaagagc caacggaact aaccgccatg
     4261 acctatctta taaagatagc gagcgattca tgttagtgat ccgagaagca atctttagca
     4321 ggattgcctg caacgacatt cttatcgtga taatgatact cccatacagt caatagtccg
     4381 agcgtgataa aaccgtcgca ggcaatgagt atggctacat gagaaccatg caggggtgga
     4441 actcccgtga gctttgctaa agctgttcga ttgctggtaa aacaactttt atgaaataca
     4501 aataagtgat ttggaaagga ggattttatg aagcagactg acattcctat ttgggaacgt
     4561 tataccctaa ccattgaaga agcgtcaaaa tattttcgta ttggcgaaaa caagctacga
     4621 cgcttggcag aggaaaataa aaatgcaaat tggctgatta tgaatggcaa tcgtattcag
     4681 attaaacgaa aacaatttga aaaaattata gatacattgg acgcaatcta gcgtcgccaa
     4741 agggtcttgt atatgataaa atagtattaa gtcgtatcaa ggctctttcc ataaaggaaa
     4801 ggagcaaatg ccatgtcaga aaaaagacgt gacaataaag gtcgaatctt aaagactgga
     4861 gagagccaac gaaaagacgg aagatactta tacaaatata tagattcatt tggagaaccg
     4921 caatttgttt actcgtggaa acttgtggct acagaccgag taccagcagg aaagcgtgat
     4981 tgtatctcac ttagagagaa aatcgcagag ttacagaaag acattcatga tggtattgat
     5041 gttgtaggaa agaaaatgac actctgccag ctttacgcaa aacagaacgc tcaaagacca
     5101 aaggttagaa aaaacactga aactggacgc aaatatctta tggatatttt gaagaaagac
     5161 aagttaggtg taagaagtat tgacagtatt aagccatcag acgctaaaga atgggctatt
     5221 agaatgagtg aaaatggtta tgcttatcaa accatcaata actacaaacg ttctttaaag
     5281 gcttcattct atattgctat acaagatgat tgtgttcgga agaatccatt tgactttcaa
     5341 ctgaaagcag ttcttgatga tgatactgtc cctaagaccg tactaacaga agaacaggaa
     5401 gaaaaactgt tagcctttgc aaaagctgat aaaacctaca gcaaaaatta tgatgaaatt
     5461 ctgatactct taaaaacagg tcttcgtatt tcagagtttg gtggtttgac acttccagat
     5521 ttagattttg agaatcgtct tgtcaatata gaccatcagc tattgagaga tactgaaatt
     5581 gggtactaca ttgaaacacc aaagaccaaa agtggcgaac gtcaagttcc tatggttgaa
     5641 gaagcctatc aagcatttaa gcgagtgtta gcgaatcgaa agaatgataa gcgtgttgag
     5701 attgatggat atagtgattt cctctttctt aatagaaaga actatccaaa agtggcaagt
     5761 gattacaacg gcatgatgaa aggtcttgtt aagaaataca ataagtataa cgaggataaa
     5821 ttgccacaca tcactccaca tagtttgcga catacattct gtaccaacta tgcaaatgca
     5881 ggaatgaatc caaaggcatt acagtacatt atgggacatg ctaatatagc catgacgctg
     5941 aactattacg cacatgcaac attcgattct gcaatggcag aaatgaaacg cttgaataaa
     6001 gagaagcaac aggagcgtct tgttgcttag tagtacaaat gaatttacta cttatttacc
     6061 acttctgaca gctaagacat gaggaaatat tcaaagaaac gtgaagtatc ttcctacagt
     6121 aaaaatactc gaaagcacat agaataaggc tttacgagca tttaagaaaa tataaaaaga
     6181 taattagaaa tttatacttt gttttactta aaaataaatg ttctttttca tttattgatc
     6241 cactgcgatt aatttcatca tctttattat gggttataca gtaagtgatt tcatcattat
     6301 ctgaaaaaat aattttcaca gtactacgcc aatcacctgg cattgttgtt gttattggat
     6361 tggaaatttg aatctcctta atatctgaat atgaattttt caatgctttg actagactaa
     6421 cctcatattc tcgattccgt tttagttctt cttttctttg tt
//
LOCUS       DATRSG010000173         4780 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699598-rid18550253.guided.095, whole genome shotgun sequence.
ACCESSION   DATRSG010000173 DATRSG010000000
VERSION     DATRSG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699598
            Sequence Read Archive: SRR7788385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789892.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789892.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:56:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,096
            CDSs (total)                      :: 2,053
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 45 of 63
            Pseudo Genes (incomplete)         :: 6 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699598-rid18550253.guided.095"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2011-03-19"
                     /collected_by="RMPRU"
     gene            17..1153
                     /gene="dnaN"
                     /locus_tag="VBD89_002091"
     CDS             17..1153
                     /gene="dnaN"
                     /locus_tag="VBD89_002091"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO7136550.1"
                     /translation="MIHFSINKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAII
                     LTGSNGQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQH
                     QIVLTSGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRP
                     ILTGVHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDD
                     IETVEVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAME
                     RAYLISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYL
                     IESLKAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            1211..2104
                     /locus_tag="VBD89_002092"
     CDS             1211..2104
                     /locus_tag="VBD89_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO7136551.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGKIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYR
                     LGKITYIVIAIKSLLHSSKVQVLVEGETGQRIKLNDLYFFALANNTYFGGGITIWPKA
                     SALTAELDMVYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEI
                     DGEIVELDQISLKCQKRYLYM"
     gene            2114..2311
                     /locus_tag="VBD89_002093"
     CDS             2114..2311
                     /locus_tag="VBD89_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO7136552.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            2917..3390
                     /gene="dfrF"
                     /locus_tag="VBD89_002094"
     CDS             2917..3390
                     /gene="dfrF"
                     /locus_tag="VBD89_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HEO7136553.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            3497..4027
                     /locus_tag="VBD89_002095"
     CDS             3497..4027
                     /locus_tag="VBD89_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7136554.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            4573..>4780
                     /locus_tag="VBD89_002096"
     CDS             4573..>4780
                     /locus_tag="VBD89_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HEO7136555.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAY"
BASE COUNT         1700 a          672 c          906 g         1502 t
ORIGIN      
        1 atataaagga tatactatga ttcatttttc aataaataaa aatttcttct tgcatgctct
       61 aacagtaacc aaacgagcta ttagtcataa aaatgcgatt ccaatccttt caactgttaa
      121 aatagaagtg actagagatg ctatcatttt aacggggtca aatggacaaa tttcaattga
      181 aaatactatt cctgcttcaa atgaaaatgc aggtttacta gtaacgaatc caggctctat
      241 tttgttagaa gctggtttct ttattaatat tatttcaagt ttaccagatg taactttaga
      301 atttacagag attgaacaac atcaaattgt tcttactagt ggaaaatcag agattacttt
      361 gaaaggtaag gatgtcgatc aataccctcg tctgcaggaa atgacaacag atactccatt
      421 aacattagaa actaaactgt taaaatcaat tattaatgaa actgcttttg ctgctagcca
      481 acaagaaagc cgtccaatct taacaggtgt tcatttggtt atcagtcaaa ataaatactt
      541 taaggctgtt gcgacagatt cacaccgtat gagtcaacgc actttccaat tagagaaatc
      601 ggctaataat tttgatttgg ttgttccaag taaatccctt cgagaatttt cggctgtttt
      661 tacagatgat attgaaactg tagaggtttt cttctcagat agtcaaatgt tatttagaag
      721 tgaaaatatc agcttctata cacgtttgct tgaaggaaac taccctgata ctgatcgcct
      781 cctaactaat cagtttgaaa ccgaaattat ctttaataca aatgctttac gccatgctat
      841 ggaacgtgct tatttaattt cgaatgcaac tcagaacggt actgttcgtt tagaaattca
      901 aaatgaaaca gtctcagctc atgtaaactc tccagaagtt ggtaaagtta atgaggaatt
      961 ggatactgtt agccttaaag gtgatagttt aaatattagt tttaatccaa cttacctaat
     1021 tgaatcttta aaagcagtaa aaagcgaaac agttacgatt cgatttattt ctccagtacg
     1081 tccatttact ttgacccctg gtgaagatac tgaagatttc atccaattaa taactcctgt
     1141 tcgtactaac taaataagaa aactacccaa gttattcttg ggtagttttc ttattctaag
     1201 atatgctata atgatgagta atatgactct atatataata gctaaccccc atgctggtaa
     1261 taaaaatgcc tccactattg ttggtaaaat tcaggagctt tatcatactg aagatatttc
     1321 tgtgttctat acagaacaga aagatgatga aaaaaaacaa gtcattaata tactaaggtc
     1381 ttttaaagaa agtgatcatc taatgattat aggaggagat ggtaccttat caaaagtaat
     1441 gacttatctc ccccaacata ttccgtgcgc ttattatcct gttggttcag gaaatgattt
     1501 tgccagggct ttgaaaattc ctaacctcaa agaaaccctt accgctattc agacagaaag
     1561 attaaaggaa attaattgct ttatttatga taaagggctg attttgaata gtttagattt
     1621 aggttttgca gcctatgtcg tttggaaagc atctaactct aaaataaaaa atattttgaa
     1681 tagatatcgt ctaggaaaaa taacttacat tgttatagct atcaaatcat tattacattc
     1741 ttctaaagta caagtattag tggaaggaga gacaggacag cggataaaac taaatgacct
     1801 ttattttttc gcattagcaa ataacactta ttttggaggt ggcattacta tttggcctaa
     1861 agcaagtgct ttaactgcag agttagatat ggtttacgct aaagggcata cttttctaaa
     1921 acgattaagt attttattat ctttggtttt taaaaggcat actacctcta aaagtataaa
     1981 acaccagact ttcaaagcta tgaccgtata ttttcctaaa aatagtttga ttgaaataga
     2041 tggcgaaata gttgagttag atcagattag tttaaaatgt caaaaaaggt atttatatat
     2101 gtaaggagaa aagatgtatc aagttggatc acttattgaa atgaaaaaac ctcatgcttg
     2161 tgtaattaaa gaaactggta aaaaggctaa tcaatggaag gtactgagag taggagctga
     2221 tattaaaatt cagtgtacta actgtcagca tgtgattatg atgagtcgtt atgattttga
     2281 gcgaaaatta aaaaaagtac tgcaaccatg aaagtccttt taaacaaata attatagcta
     2341 tatttgcaac caatactttc ctaaataatt gctagtatgc cgttggaaaa ttagctattc
     2401 taatgttatc gaaagaagaa aggtggccat tgacaatgac gaaagttgca gaacagttgt
     2461 ttatttttcg gtaagtcata tcaaaatggg aaaatggtgt ttcccaaaca aaaagatatt
     2521 aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat attgctacta gactaagaga
     2581 tactacataa gtgtgttggt atctcttttt tgttgccgtg aaacagcaga ctatttcatt
     2641 tctgcttgat taccttaaaa cttattagtc tatggtttgt caagggcttg ttgcgtgaga
     2701 aatgctgaat atcacccttt acaaaacata ggctgataag taacttttca aaagcagaaa
     2761 agaaatggca atgcttataa caatatgata taatgcagat gaagacaaaa aaggaatttg
     2821 tcgagctggt tagttcgaga taattttgag tttgttaaga tgtggtgcac ttgatggaat
     2881 cacaggtacg acatcatatt atggaggatg aaatagatga taggtttgat tgttgcgagg
     2941 tcaaagaata atgttatagg caagaatggt aatataccat ggaaaataaa gggagaacaa
     3001 aagcaattta gagagttaac aacgggtaat gtggttatta tggggcgaaa gtcttatgaa
     3061 gaaatcggtc atccgttgcc taatagaatg aatattgttg tttccaccac aacagagtat
     3121 caaggagata atttagtttc agttaaatca ttagaagatg cattattatt ggctaaagga
     3181 cgagatgtat acatatctgg tggatatgga ctatttaagg aagctttgca aatagtagat
     3241 aaaatgtata tcacagaagt agatttaaat attgaagatg gagatacatt ctttccagaa
     3301 tttgatatca atgattttga agttttgata ggggaaacac ttggtgagga agtgaaatat
     3361 acgagaacat tttatgtaag gaaaaaatga attgagtaaa ttttggattt agggtgtttt
     3421 cataaatata ttgcttttta tgcatatata aattgttgta agacaaattc ctgtttgtat
     3481 ttataaaaga gaaaaaatga gaaaagtagt tttatttatt gccatgagcc ttgacggata
     3541 tattgcggac ggtaatggtg gagtagcttg gctaaatggt catggaaatg acaatgaaaa
     3601 catcgacact tatacagaat ttaccaaaga tatagatact gtcttaatgg ggtggaatac
     3661 ttctcatcag gttgttactg aactttctcc gcaagaatgg gtatataata aatttacaac
     3721 ttatgtatta acacataaag agtgcaattc ttcccaacaa attcatttta caagtgaaaa
     3781 tcctgtttta ttattggaac ggctaaaaca agaagcagga aaggatattt ggatttgtgg
     3841 cggagcaaat cttgttcagc agttggtaag caaaaatatg attgataaat attacatttc
     3901 tgttattcct actctgttag gaaatggggt tcgtcttttg ggcaacatag acagggaaat
     3961 aaagttaagg ctttgtaaaa cacagactta taacggaata accgatttga tttatatgcg
     4021 tagataacta gagagaaaca gaaaagctgt taaaccgacg gagcgatccg accacgttgg
     4081 cgggaaaatc cttaagattt ttccgccatg cttgcaaggg ggcggttcgc ttcggcggtt
     4141 cggcgagctt gcgagccgtt caaagccccc gttatctaac aaggggggca caaaaaacaa
     4201 gaaacactat acactaacaa gcggaaaacg ctgtatttat aaggcaaaac cccattttta
     4261 cagtagtgat ataaattgta cctatatcac tatgaaaatt gaatagtaaa tatgagaaag
     4321 acagttgatt tcaaaaaata agaaattgat tgtcgttttc ttttgcagaa atttaagtgc
     4381 aggcaattat ggtagttgtc gtaattgctg tcagctcagt gtttatcact gatggtgtga
     4441 aagatttacg gttggagatg ttcaataaga ttattggtgc ttaattattt ttattgtagt
     4501 gctgatatga gaatgttata attggggtat aaaacataca aataaatttg ctgaaaaatg
     4561 aggtatcaaa atatggaaaa tgaattactg gaaaacttag gaagacttca cacaactgaa
     4621 cttggcgtta ttagaattaa aaagaactta tctttgaatg tagaaaatgt aattgaatgg
     4681 tgcaaagaaa aaattagcct ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat
     4741 ataacgattg ataattgtat tataacggta aatgcgtaca
//
